LOCUS       DAJVPP010000001        94813 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVPP010000001 DAJVPP010000000
VERSION     DAJVPP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 94813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.001"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1145)
                     /gene="icd"
                     /locus_tag="OQM34_000001"
     CDS             complement(<1..1145)
                     /gene="icd"
                     /locus_tag="OQM34_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1450601.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEG"
     gene            1317..1970
                     /gene="rluE"
                     /locus_tag="OQM34_000002"
     CDS             1317..1970
                     /gene="rluE"
                     /locus_tag="OQM34_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1450602.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGTELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            1980..2441
                     /gene="nudJ"
                     /locus_tag="OQM34_000003"
     CDS             1980..2441
                     /gene="nudJ"
                     /locus_tag="OQM34_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1450603.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2495..3601
                     /gene="mnmA"
                     /locus_tag="OQM34_000004"
     CDS             2495..3601
                     /gene="mnmA"
                     /locus_tag="OQM34_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1450604.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3637..4278
                     /gene="hflD"
                     /locus_tag="OQM34_000005"
     CDS             3637..4278
                     /gene="hflD"
                     /locus_tag="OQM34_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1450605.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4282..5652
                     /gene="purB"
                     /locus_tag="OQM34_000006"
     CDS             4282..5652
                     /gene="purB"
                     /locus_tag="OQM34_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1450606.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5821..6492
                     /gene="phoP"
                     /locus_tag="OQM34_000007"
     CDS             5821..6492
                     /gene="phoP"
                     /locus_tag="OQM34_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1450607.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6492..7952
                     /gene="phoQ"
                     /locus_tag="OQM34_000008"
     CDS             6492..7952
                     /gene="phoQ"
                     /locus_tag="OQM34_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1450608.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8749..9030)
                     /locus_tag="OQM34_000009"
     CDS             complement(8749..9030)
                     /locus_tag="OQM34_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450609.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9044..10705)
                     /locus_tag="OQM34_000010"
     CDS             complement(9044..10705)
                     /locus_tag="OQM34_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1450610.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10689..11015)
                     /locus_tag="OQM34_000011"
     CDS             complement(10689..11015)
                     /locus_tag="OQM34_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1450611.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11071..11253)
                     /locus_tag="OQM34_000012"
     CDS             complement(11071..11253)
                     /locus_tag="OQM34_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450612.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11237..11677)
                     /locus_tag="OQM34_000013"
     CDS             complement(11237..11677)
                     /locus_tag="OQM34_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1450613.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11677..11973)
                     /locus_tag="OQM34_000014"
     CDS             complement(11677..11973)
                     /locus_tag="OQM34_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1450614.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(11970..12308)
                     /locus_tag="OQM34_000015"
     CDS             complement(11970..12308)
                     /locus_tag="OQM34_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1450615.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12305..13516)
                     /locus_tag="OQM34_000016"
     CDS             complement(12305..13516)
                     /locus_tag="OQM34_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1450616.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVDQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13518..14090)
                     /locus_tag="OQM34_000017"
     CDS             complement(13518..14090)
                     /locus_tag="OQM34_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1450617.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDPSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14130..15287)
                     /locus_tag="OQM34_000018"
                     /pseudo
     CDS             complement(14130..15287)
                     /locus_tag="OQM34_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15588..15812)
                     /locus_tag="OQM34_000019"
     CDS             complement(15588..15812)
                     /locus_tag="OQM34_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450618.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(15954..16115)
                     /locus_tag="OQM34_000020"
     CDS             complement(15954..16115)
                     /locus_tag="OQM34_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1450619.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16125..16430)
                     /locus_tag="OQM34_000021"
     CDS             complement(16125..16430)
                     /locus_tag="OQM34_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450620.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16427..16705)
                     /locus_tag="OQM34_000022"
     CDS             complement(16427..16705)
                     /locus_tag="OQM34_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450621.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16994..18742)
                     /locus_tag="OQM34_000023"
     CDS             complement(16994..18742)
                     /locus_tag="OQM34_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1450622.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIAILLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18739..19038)
                     /locus_tag="OQM34_000024"
     CDS             complement(18739..19038)
                     /locus_tag="OQM34_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1450623.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19044..19277)
                     /locus_tag="OQM34_000025"
     CDS             complement(19044..19277)
                     /locus_tag="OQM34_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450624.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19279..19499)
                     /locus_tag="OQM34_000026"
                     /pseudo
     CDS             complement(19279..19499)
                     /locus_tag="OQM34_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19492..19713)
                     /locus_tag="OQM34_000027"
     CDS             complement(19492..19713)
                     /locus_tag="OQM34_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450625.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19703..19903)
                     /locus_tag="OQM34_000028"
     CDS             complement(19703..19903)
                     /locus_tag="OQM34_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1450626.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20033..20212
                     /locus_tag="OQM34_000029"
     CDS             20033..20212
                     /locus_tag="OQM34_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450627.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20337..20666
                     /locus_tag="OQM34_000030"
     CDS             20337..20666
                     /locus_tag="OQM34_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450628.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20716..20904)
                     /locus_tag="OQM34_000031"
     CDS             complement(20716..20904)
                     /locus_tag="OQM34_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1450629.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21279..22508)
                     /locus_tag="OQM34_000032"
     CDS             complement(21279..22508)
                     /locus_tag="OQM34_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1450630.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLNLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGVQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22757..23878
                     /gene="roxA"
                     /locus_tag="OQM34_000033"
                     /gene_synonym="ycfD"
     CDS             22757..23878
                     /gene="roxA"
                     /locus_tag="OQM34_000033"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1450631.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23927..25153)
                     /gene="pepT"
                     /locus_tag="OQM34_000034"
     CDS             complement(23927..25153)
                     /gene="pepT"
                     /locus_tag="OQM34_000034"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1450632.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25403..26539
                     /gene="potA"
                     /locus_tag="OQM34_000035"
     CDS             25403..26539
                     /gene="potA"
                     /locus_tag="OQM34_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1450633.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26523..27380
                     /gene="potB"
                     /locus_tag="OQM34_000036"
     CDS             26523..27380
                     /gene="potB"
                     /locus_tag="OQM34_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1450634.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27377..28171
                     /gene="potC"
                     /locus_tag="OQM34_000037"
     CDS             27377..28171
                     /gene="potC"
                     /locus_tag="OQM34_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1450635.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28168..29214
                     /gene="potD"
                     /locus_tag="OQM34_000038"
     CDS             28168..29214
                     /gene="potD"
                     /locus_tag="OQM34_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1450636.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29370..30191)
                     /gene="cobB"
                     /locus_tag="OQM34_000039"
     CDS             complement(29370..30191)
                     /gene="cobB"
                     /locus_tag="OQM34_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1450637.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30207..31118)
                     /gene="nagK"
                     /locus_tag="OQM34_000040"
     CDS             complement(30207..31118)
                     /gene="nagK"
                     /locus_tag="OQM34_000040"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1450638.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31147..32391)
                     /gene="lolE"
                     /locus_tag="OQM34_000041"
     CDS             complement(31147..32391)
                     /gene="lolE"
                     /locus_tag="OQM34_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1450639.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32391..33092)
                     /gene="lolD"
                     /locus_tag="OQM34_000042"
     CDS             complement(32391..33092)
                     /gene="lolD"
                     /locus_tag="OQM34_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1450640.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33085..34284)
                     /gene="lolC"
                     /locus_tag="OQM34_000043"
     CDS             complement(33085..34284)
                     /gene="lolC"
                     /locus_tag="OQM34_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1450641.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLMPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34546..35619
                     /locus_tag="OQM34_000044"
     CDS             34546..35619
                     /locus_tag="OQM34_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1450642.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFIYTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35747..39193
                     /gene="mfd"
                     /locus_tag="OQM34_000045"
     CDS             35747..39193
                     /gene="mfd"
                     /locus_tag="OQM34_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1450643.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39336..40298
                     /gene="ldtC"
                     /locus_tag="OQM34_000046"
     CDS             39336..40298
                     /gene="ldtC"
                     /locus_tag="OQM34_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCS1450644.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40381..40593)
                     /locus_tag="OQM34_000047"
     CDS             complement(40381..40593)
                     /locus_tag="OQM34_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1450645.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40878..41510
                     /gene="comR"
                     /locus_tag="OQM34_000048"
     CDS             40878..41510
                     /gene="comR"
                     /locus_tag="OQM34_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1450646.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41572..42110)
                     /locus_tag="OQM34_000049"
                     /pseudo
     CDS             complement(41572..42110)
                     /locus_tag="OQM34_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
     gene            complement(42337..43641)
                     /gene="ndh"
                     /locus_tag="OQM34_000050"
     CDS             complement(42337..43641)
                     /gene="ndh"
                     /locus_tag="OQM34_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1450647.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44049..44591)
                     /locus_tag="OQM34_000051"
     CDS             complement(44049..44591)
                     /locus_tag="OQM34_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1450648.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44614..45639)
                     /gene="nagZ"
                     /locus_tag="OQM34_000052"
     CDS             complement(44614..45639)
                     /gene="nagZ"
                     /locus_tag="OQM34_000052"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1450649.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(45650..46474)
                     /gene="thiK"
                     /locus_tag="OQM34_000053"
     CDS             complement(45650..46474)
                     /gene="thiK"
                     /locus_tag="OQM34_000053"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1450650.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46455..47096)
                     /gene="lpoB"
                     /locus_tag="OQM34_000054"
     CDS             complement(46455..47096)
                     /gene="lpoB"
                     /locus_tag="OQM34_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1450651.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47111..47488)
                     /locus_tag="OQM34_000055"
     CDS             complement(47111..47488)
                     /locus_tag="OQM34_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1450652.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47491..47850)
                     /gene="hinT"
                     /locus_tag="OQM34_000056"
     CDS             complement(47491..47850)
                     /gene="hinT"
                     /locus_tag="OQM34_000056"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1450653.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            47981..48168
                     /locus_tag="OQM34_000057"
                     /pseudo
     CDS             47981..48168
                     /locus_tag="OQM34_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            48201..50386
                     /gene="fhuE"
                     /locus_tag="OQM34_000058"
                     /pseudo
     CDS             48201..50386
                     /gene="fhuE"
                     /locus_tag="OQM34_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50446..51879)
                     /gene="ptsG"
                     /locus_tag="OQM34_000059"
     CDS             complement(50446..51879)
                     /gene="ptsG"
                     /locus_tag="OQM34_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1450654.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52174..52971)
                     /locus_tag="OQM34_000060"
     CDS             complement(52174..52971)
                     /locus_tag="OQM34_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1450655.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52982..53986)
                     /gene="holB"
                     /locus_tag="OQM34_000061"
     CDS             complement(52982..53986)
                     /gene="holB"
                     /locus_tag="OQM34_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1450656.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLLREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53983..54624)
                     /locus_tag="OQM34_000062"
     CDS             complement(53983..54624)
                     /locus_tag="OQM34_000062"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1450657.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54614..55636)
                     /gene="yceG"
                     /locus_tag="OQM34_000063"
     CDS             complement(54614..55636)
                     /gene="yceG"
                     /locus_tag="OQM34_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1450658.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55639..56448)
                     /gene="pabC"
                     /locus_tag="OQM34_000064"
     CDS             complement(55639..56448)
                     /gene="pabC"
                     /locus_tag="OQM34_000064"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1450659.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56568..57809)
                     /gene="fabF"
                     /locus_tag="OQM34_000065"
     CDS             complement(56568..57809)
                     /gene="fabF"
                     /locus_tag="OQM34_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1450660.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57896..58132)
                     /gene="acpP"
                     /locus_tag="OQM34_000066"
     CDS             complement(57896..58132)
                     /gene="acpP"
                     /locus_tag="OQM34_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1450661.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58343..59077)
                     /gene="fabG"
                     /locus_tag="OQM34_000067"
     CDS             complement(58343..59077)
                     /gene="fabG"
                     /locus_tag="OQM34_000067"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1450662.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59090..60019)
                     /gene="fabD"
                     /locus_tag="OQM34_000068"
     CDS             complement(59090..60019)
                     /gene="fabD"
                     /locus_tag="OQM34_000068"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1450663.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60035..60988)
                     /gene="fabH"
                     /locus_tag="OQM34_000069"
     CDS             complement(60035..60988)
                     /gene="fabH"
                     /locus_tag="OQM34_000069"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1450664.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61056..62126)
                     /gene="plsX"
                     /locus_tag="OQM34_000070"
     CDS             complement(61056..62126)
                     /gene="plsX"
                     /locus_tag="OQM34_000070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1450665.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62207..62380)
                     /gene="rpmF"
                     /locus_tag="OQM34_000071"
     CDS             complement(62207..62380)
                     /gene="rpmF"
                     /locus_tag="OQM34_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1450666.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62432..62953)
                     /gene="yceD"
                     /locus_tag="OQM34_000072"
     CDS             complement(62432..62953)
                     /gene="yceD"
                     /locus_tag="OQM34_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1450667.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63152..63736
                     /gene="yceF"
                     /locus_tag="OQM34_000073"
     CDS             63152..63736
                     /gene="yceF"
                     /locus_tag="OQM34_000073"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1450668.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63848..64807)
                     /gene="rluC"
                     /locus_tag="OQM34_000074"
     CDS             complement(63848..64807)
                     /gene="rluC"
                     /locus_tag="OQM34_000074"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1450669.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65380..68565
                     /gene="rne"
                     /locus_tag="OQM34_000075"
     CDS             65380..68565
                     /gene="rne"
                     /locus_tag="OQM34_000075"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1450670.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68762..69715)
                     /gene="flgL"
                     /locus_tag="OQM34_000076"
     CDS             complement(68762..69715)
                     /gene="flgL"
                     /locus_tag="OQM34_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS1450671.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(69727..71369)
                     /gene="flgK"
                     /locus_tag="OQM34_000077"
                     /pseudo
     CDS             complement(69727..71369)
                     /gene="flgK"
                     /locus_tag="OQM34_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71435..72376)
                     /gene="flgJ"
                     /locus_tag="OQM34_000078"
     CDS             complement(71435..72376)
                     /gene="flgJ"
                     /locus_tag="OQM34_000078"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCS1450672.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72376..73473)
                     /gene="flgI"
                     /locus_tag="OQM34_000079"
     CDS             complement(72376..73473)
                     /gene="flgI"
                     /locus_tag="OQM34_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1450673.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73485..74183)
                     /gene="flgH"
                     /locus_tag="OQM34_000080"
     CDS             complement(73485..74183)
                     /gene="flgH"
                     /locus_tag="OQM34_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1450674.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74236..75018)
                     /gene="flgG"
                     /locus_tag="OQM34_000081"
                     /pseudo
     CDS             complement(74236..75018)
                     /gene="flgG"
                     /locus_tag="OQM34_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(75156..75907)
                     /gene="flgF"
                     /locus_tag="OQM34_000082"
                     /pseudo
     CDS             complement(75156..75907)
                     /gene="flgF"
                     /locus_tag="OQM34_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(75927..77135)
                     /gene="flgE"
                     /locus_tag="OQM34_000083"
     CDS             complement(75927..77135)
                     /gene="flgE"
                     /locus_tag="OQM34_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1450675.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77160..77855)
                     /gene="flgD"
                     /locus_tag="OQM34_000084"
     CDS             complement(77160..77855)
                     /gene="flgD"
                     /locus_tag="OQM34_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS1450676.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77867..78270)
                     /gene="flgC"
                     /locus_tag="OQM34_000085"
                     /pseudo
     CDS             complement(77867..78270)
                     /gene="flgC"
                     /locus_tag="OQM34_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78274..78690)
                     /gene="flgB"
                     /locus_tag="OQM34_000086"
     CDS             complement(78274..78690)
                     /gene="flgB"
                     /locus_tag="OQM34_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS1450677.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSFDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78845..79504
                     /gene="flgA"
                     /locus_tag="OQM34_000087"
     CDS             78845..79504
                     /gene="flgA"
                     /locus_tag="OQM34_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1450678.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            79580..79873
                     /gene="flgM"
                     /locus_tag="OQM34_000088"
     CDS             79580..79873
                     /gene="flgM"
                     /locus_tag="OQM34_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS1450679.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79878..80294
                     /gene="flgN"
                     /locus_tag="OQM34_000089"
     CDS             79878..80294
                     /gene="flgN"
                     /locus_tag="OQM34_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1450680.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80334..81869)
                     /gene="murJ"
                     /locus_tag="OQM34_000090"
     CDS             complement(80334..81869)
                     /gene="murJ"
                     /locus_tag="OQM34_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1450681.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82036..82902)
                     /locus_tag="OQM34_000091"
     CDS             complement(82036..82902)
                     /locus_tag="OQM34_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1450682.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(82904..83551)
                     /locus_tag="OQM34_000092"
     CDS             complement(82904..83551)
                     /locus_tag="OQM34_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS1450683.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLYSLLAHLGD"
     gene            complement(83562..84146)
                     /gene="rimJ"
                     /locus_tag="OQM34_000093"
     CDS             complement(83562..84146)
                     /gene="rimJ"
                     /locus_tag="OQM34_000093"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1450684.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84382..85590
                     /gene="mdtH"
                     /locus_tag="OQM34_000094"
     CDS             84382..85590
                     /gene="mdtH"
                     /locus_tag="OQM34_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1450685.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85654..86301
                     /gene="grxB"
                     /locus_tag="OQM34_000095"
     CDS             85654..86301
                     /gene="grxB"
                     /locus_tag="OQM34_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1450686.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86435..86995
                     /locus_tag="OQM34_000096"
     CDS             86435..86995
                     /locus_tag="OQM34_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1450687.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87101..88147
                     /gene="pyrC"
                     /locus_tag="OQM34_000097"
     CDS             87101..88147
                     /gene="pyrC"
                     /locus_tag="OQM34_000097"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1450688.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88221..88466
                     /gene="dinI"
                     /locus_tag="OQM34_000098"
     CDS             88221..88466
                     /gene="dinI"
                     /locus_tag="OQM34_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1450689.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88756..89010
                     /gene="bssS"
                     /locus_tag="OQM34_000099"
     CDS             88756..89010
                     /gene="bssS"
                     /locus_tag="OQM34_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1450690.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89125..90243
                     /gene="solA"
                     /locus_tag="OQM34_000100"
     CDS             89125..90243
                     /gene="solA"
                     /locus_tag="OQM34_000100"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1450691.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90291..90404
                     /locus_tag="OQM34_000101"
     CDS             90291..90404
                     /locus_tag="OQM34_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCS1450692.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90665..91231
                     /locus_tag="OQM34_000102"
     CDS             90665..91231
                     /locus_tag="OQM34_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS1450693.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91235..91810
                     /locus_tag="OQM34_000103"
     CDS             91235..91810
                     /locus_tag="OQM34_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1450694.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91852..92904)
                     /locus_tag="OQM34_000104"
     CDS             complement(91852..92904)
                     /locus_tag="OQM34_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS1450695.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93129..94049
                     /gene="lpxL"
                     /locus_tag="OQM34_000105"
     CDS             93129..94049
                     /gene="lpxL"
                     /locus_tag="OQM34_000105"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1450696.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94221..94418
                     /locus_tag="OQM34_000106"
     CDS             94221..94418
                     /locus_tag="OQM34_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS1450697.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94442..>94813
                     /locus_tag="OQM34_000107"
     CDS             94442..>94813
                     /locus_tag="OQM34_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1450698.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRES"
BASE COUNT        22764 a        25163 c        23418 g        23468 t
ORIGIN      
        1 ccttccatac ctttaacaat caggtctgcg gcttcagtcc agcccatatg gcgtaacatc
       61 atctcagcgg agagaataat agagccaggg tttactttgt cctgaccggc gtatttcggc
      121 gcagtaccgt gggtggcttc aaacagggcg cattcgtcac cgatgtttgc tccaggggcg
      181 ataccgatac cgccaacctg cgctgccaga gcgtcagaaa tgtagtcacc gttcaggttc
      241 atacaggcga taacatcata ttcagccgga cgcagcagga tctgttgcag gaatgcatca
      301 gcaatcacgt ctttaatgac gatctctttg ccggtattcg ggtttttaac tttcagccac
      361 gggccgccgt cgatcagttc accgccaaat tcttcacgcg ccagctggta gccccagtct
      421 ttaaacgcgc cttcggtgaa cttcatgatg ttgcctttgt gcaccagggt cacagagtca
      481 cgatcgttag caattgcgta ttcgatcgct gcacgaacca gacgtttggt gccttcttca
      541 gaacacggct taataccgat accgcaatgt tccgggaagc gaattttctt cacgcccatc
      601 tcttcacgca ggaatttaat cactttctcg gcgtcggcag agtcagcttt ccattcgata
      661 cccgcataaa tgtcttccga gttttcacgg aagataacca tatcggtcag ttcagggtgt
      721 ttaaccgggc ttggagtgcc ctgatagtaa cgtaccggac gcaggcagat gtagagatcc
      781 agttcctgac gcagggcaac gttgagagaa cgaataccgc caccaaccgg agtggtcagc
      841 gggcctttaa tggcaacgcg atattcacga atcagatcaa gggtttcagc aggcagccag
      901 acatcctgac cataaacctg tgtggatttt tcaccggtgt aaatttccat ccaggagatt
      961 ttacgctcgc ctttataggc tttctcgact gcagcgtcga ccactttcag catggctggg
     1021 gttacatcta caccgattcc atcaccttca atgtaaggga taatcggatt ttcaggaacg
     1081 ttgagtttgc cgttttgcag ggtgatcttc ttgccttgtg ccggaacaac tactttactt
     1141 tccattcacc tctccttcga gcgctactgg tttgctcgtc tgccaccacg ttgcatatgc
     1201 gtttgcgtcc tgcgatacgg atgctttaga gcaatttttt gttaatgatt tgtaattgac
     1261 ttgtcaatac taccctattg tttgccgtca tgaaagatgc gcgttattag gctataatgc
     1321 ggcaattcat aatctctgaa aataccatgc aaaaaacttc ttttagaaat caccaggtta
     1381 agcgattcag ctcgcaacgt tctaccaggc gtaaacatga aaaccagccc acgcgtgtga
     1441 tcctcttcaa taaaccctac gatgttcttc cgcagttcac cgatgaaacc ggacgcaaaa
     1501 cattaaaaga attcatcccg gttcagggcg tttatgcagc aggtcgcctt gaccgcgata
     1561 gcgaagggtt gctggtgctg accaataacg gcgcgttgca ggcgcgttta acccagccgg
     1621 gtaaacgcac cggaaaaatc tattatgtgc aggtggaagg tattcccaca caagacgcac
     1681 ttgaagcctt gcgcaatggc gtaaccttaa atgatggccc taccctgccc gcaggcacgg
     1741 agctggttga cgaacccgcg tggttatggc cgcggaatcc accaattcgt gaacgcaaaa
     1801 gtattcccac cagctggctg aagatcacct tatatgaagg acgtaatcgc caggtgcgcc
     1861 gcatgaccgc ccatgttggt ttccccacgc tgcgactgat tcgctatgcg atgggagatt
     1921 actctttgga taatcttgcc aatggcgaat ggcgagaagt gacagattaa ggatgcaaaa
     1981 tgtttaaacc gcacgttacc gttgcttgcg tggtgcacgc agaaggcaaa tttttagtcg
     2041 ttgaagagac gattaatggt aaagcgttat ggaaccaacc tgccgggcat ctggaagccg
     2101 atgaaacctt agtggaagcc gccgcccgtg aactgtggga agaaaccggc atcagcgcgc
     2161 agccgcaaca ctttattcgt atgcatcagt ggattgcgcc agataaaacg ccgtttttgc
     2221 gtttcctctt tgccattgag cttgagcaaa tatgcccgac ccagcctcat gacagcgata
     2281 tcgactgctg ccgttgggtc agcgccgaag aaattttaca ggcgtcaaat cttcgttcgc
     2341 cgctggtggc ggaaagtatt cgttgttatc aaagcgggca acgttatccg ctggagatga
     2401 ttggcgattt taactggcct tttacaaagg gtgtcatcta aaaggctgcg tgatagaata
     2461 cgccgccttg aagttcaatg tcgtgagtga tccaatgtct gaaaccgcaa aaaaagtaat
     2521 cgtcggcatg tccggcggtg tcgattcctc cgtttctgcc tggctgttgc aacaacaggg
     2581 atatcaggtc gaaggcctgt ttatgaagaa ctgggaagaa gacgacggtg aggaatattg
     2641 cacagcggca gcggatctgg ctgatgccca ggcggtctgc gacaagctcg gcattgaact
     2701 gcacaccgtt aactttgctg ccgagtactg ggacaacgtc ttcgaactgt tccttgccga
     2761 atataaagcc ggtcgcacgc cgaatccgga tattctgtgc aacaaggaga tcaaatttaa
     2821 agccttcctc gaatttgccg ccgaagattt aggtgccgat tatatcgcta ccggtcatta
     2881 cgtacgtcgg gccgatgtcg atggcaagag ccgcctgctg cgtggtctgg acagcaataa
     2941 agaccagagc tacttccttt atacgctcag ccatgagcag attgcgcaaa gcctgttccc
     3001 ggtaggcgaa ctggaaaaac cgcaggtgcg taagattgct gaagatcttg gtctggtcac
     3061 cgcgaagaaa aaagactcta ccggcatttg cttcattggc gaacgtaaat tccgcgagtt
     3121 cctgggccgt tatctcccgg cgcaaccggg caaaatcatt accgtcgatg gcgatgaaat
     3181 tggtgagcac caggggctga tgtatcacac tctcggtcag cgtaaaggtc tgggtatcgg
     3241 tggcaccaaa gaaggcaccg aagaaccgtg gtatgtggtg gacaaagacg tcgaaaacaa
     3301 cattctggtt gtcgctcagg gccatgaaca cccgcggctg atgtctgtcg ggttgattgc
     3361 ccagcagctg cactgggtcg atcgcgaacc attcaccggc actatgcgtt gcacggtaaa
     3421 aacccgctat cgccagaccg acattccgtg caccgtcaag gcgctggacg acgatcgcat
     3481 taaagtgatt ttcgatgagc cggttgccgc cgtgacgccg ggccagtctg ccgtcttcta
     3541 taacggtgaa gtgtgcctcg gtggcggtat tattgagcag cgtctgccgc tgccggtctg
     3601 attattatct ttacttaaaa caaggaagca gtgaacgtgg caaagaatta ctatgacatc
     3661 accctcgccc tggccggtat ttgtcagtcg gcacgcctgg tgcaacaact cgctcaccag
     3721 gggcattgtg atgccgatgc gctacacgtc tcactcaaca gtattattga tatgaacccc
     3781 agctcgacgc tggcggtttt tggcggtagc gaagccaacc tgcgcgtcgg gctggaaacc
     3841 ctgctcggcg tgctcaatgc cagcagttgc cagggcttaa acgccgaatt aacccgctac
     3901 acactcagct tgatggtgct tgagcgcaaa ctctcctcag cgaaaggcgc gctcgacact
     3961 ctgggcaacc ggatcaacgg cctgcaacgc cagctcgaac acttcgattt acagtccgaa
     4021 acgctgatga gcgcgatggc tgctatctat gttgatgtga tcagcccgct tggcccgcgc
     4081 attcaggtca ccggttcccc tgctgtactg caaagcccac aagtgcaggc gaaagttcgc
     4141 gcaaccctgc tggcaggcat tcgcgccgcc gtgctctggc accaggtcgg cggcggacgt
     4201 ctacaactga tgttttctcg taatcgcctg accactcagg caaaacaaat tcttgctcat
     4261 ttaaccccgg agttgtgatc tatggaatta tcctcactga ccgccgtttc ccctgtcgat
     4321 ggacgctacg gcgataaagt cagcgcgctg cgcgggattt tcagcgaata tggtttgctg
     4381 aaattccgtg tacaagttga agtacgttgg ctgcaaaaac tggccgcgca cgcagcgatc
     4441 aaggaagttc ctgcttttgc tgccgacgca atcggttacc ttgatgcaat tgtcgccaat
     4501 ttcagcgaag aagatgccgc acgcatcaaa accatcgagc gtaccactaa ccacgacgtt
     4561 aaagcggttg agtatttcct gaaagaaaaa gtggcggaga tcccggaact gcacgcggtt
     4621 tctgaattca tccactttgc ctgtacttcg gaagatatca ataacctctc ccacgcatta
     4681 atgctgaaaa ccgcgcgtga tgaagtgatc ctgccgtact ggcgtcaact gattgatggc
     4741 attaaagatc tcgccgttca gtaccgcgat atcccgctgc tgtctcgtac ccacggtcag
     4801 ccagccacgc cgtcaaccat cggtaaagag atggcaaacg tcgcctaccg tatggagcgc
     4861 cagcaccgcc agcttaacca ggtggagatc ctcggcaaaa tcaacggcgc ggtcggtaac
     4921 tataacgccc acatcgccgc ttacccggaa gttgactggc atcagttcag cgaagagttc
     4981 gtcacctcgc tgggtattca atggaacccg tacactaccc agattgaacc gcacgactac
     5041 attgccgaac tgtttgattg cgttgcgcgc ttcaacacca ttctgatcga ctttgaccgt
     5101 gacgtctggg gttatatcgc ccttaaccac ttcaaacaga aaaccattgc tggtgagatt
     5161 ggttcttcca ccatgccgca taaagttaac ccgatcgact tcgaaaactc cgaagggaat
     5221 ctgggccttt ccaacgcggt attgcagcat ctggcaagca aactgccggt ttcccgctgg
     5281 cagcgtgacc tgaccgactc caccgtgctg cgtaacctcg gcgtgggtat cggttatgcg
     5341 ctgattgcgt atcaatccac cctgaaaggc gtgagcaaac tggaagtgaa ccgtgaccat
     5401 ctgctggatg agctggatca caactgggaa gtgctggcag aaccaatcca gacagttatg
     5461 cgtcgctatg gcatcgaaaa accgtacgag aagctgaaag agcttactcg cggtaagcgc
     5521 gttgacgccg aaggcatgaa gcagtttatc gatggtctgg cgttgccaga agaagagaaa
     5581 gcccgcctga aagcgatgac gccggctaac tatattggtc gagctatcac gatggttgat
     5641 gagctgaaat aagcctcaaa tcagtgccgg atggcgatgc tgtccggcct gcttattaag
     5701 attatccact ttttcttttt tcactttacc tcccctcccc gctggtttat ttaatgttta
     5761 cccccataaa cacataatcg cgttacacta ttttaataat taagacaggg agaaataaaa
     5821 atgcgcgtac tggttgttga agacaatgcg ttgttacgtc accaccttaa agttcagatt
     5881 caggatgctg gtcatcaggt cgatgacgca gaagatgcca aagaagccga ttattatctc
     5941 aatgaacatt taccggatat tgcgattgtc gatctcggat tgcctgacga ggacggtctg
     6001 tcactgattc gccgctggcg tagcaacgat gtttcactgc cgattctggt attaaccgcc
     6061 cgtgaaagct ggcaggacaa agtcgaagta ttaagtgccg gtgctgatga ttacgtgact
     6121 aaaccgtttc atattgaaga ggtgatggcg cgaatgcagg cattaatgcg gcgtaatagc
     6181 ggtctggctt cacaggtcat ttcgctcccc ccgtttcagg ttgatctctc tcgccgtgaa
     6241 ttatctatta atgacgaagt gatcaaactg accgcgttcg aatacaccat tatggaaacg
     6301 ttgatacgca ataatggcaa agtggtcagc aaagattcgt taatgctaca actctatccg
     6361 gatgcggagc tgcgggaaag ccataccatt gatgtactga tgggacgtct gcgcaaaaaa
     6421 attcaggcac aatatccgca agaagtcatt accaccgttc gcggccaggg ctacctgttc
     6481 gaattgcgct gatgaaaaaa ttactgcgtc tttttttccc gctctcgctg cgggtacgtt
     6541 ttctgttggc aacggcagcg gtagtactgg tgctttcgct tgcctacgga atggtcgcgc
     6601 tgatcggtta tagcgtcagt ttcgataaaa ctacgtttcg gctgttacgt ggcgagagca
     6661 atctgttcta tacccttgcg cagtgggaaa acaataagtt gcatgtcgag ttacccgaaa
     6721 atatcgacaa gcaaagcccc accatgacgc taatttatga tgagaacggg cagcttttat
     6781 gggcgcaacg tgacgtgccc tggctgatga aaatgatcca gcctgactgg ctgaaatcga
     6841 atggttttca tgaaattgaa gcggatgtta acgataccag cctcttgctg agtggagatc
     6901 attcgataca gcaacagttg caggaagtgc gggaagatga tgacgacgcg gagatgaccc
     6961 actcggtggc ggtaaacgtc tacccggcaa catcgcggat gccaaaatta accattgtgg
     7021 tggtggatac cattccggtg gagctaaaaa gttcctatat ggtctggagc tggtttatct
     7081 atgtgctctc agccaatctg ctgttagtga tcccgctgct gtgggtcgcc gcctggtgga
     7141 gtttacgccc catcgaagcc ctggcaaaag aagtccgcga actggaagaa cataaccgcg
     7201 aattgctcaa tccagccaca acgcgagaac tgaccagtct ggtacgaaac ctgaaccgat
     7261 tgttaaaaag tgaacgcgaa cgttacgaca aataccgtac gacgctcacc gacctgaccc
     7321 atagtctgaa aacgccactg gcggtgctgc aaagtacgct gcgttctctg cgtagtgaaa
     7381 agatgagcgt cagtgatgct gagccggtaa tgctggagca aatcagccgc atttcacagc
     7441 aaattggcta ctacctgcat cgtgccagta tgcgcggcgg gacattgctc agccgcgagc
     7501 tgcatccggt cgccccactg ctggacaatc tcacctcagc gctgaacaaa gtgtatcaac
     7561 gcaaaggggt caatatctct ctcgatattt cgccagagat cagctttgtc ggtgagcaga
     7621 acgattttgt cgaggtgatg ggcaacgtgc tggataatgc ctgtaaatat tgcctcgagt
     7681 ttgtcgaaat ttctgcaagg caaaccgacg agcatctcta tattgtggtc gaggatgatg
     7741 gccccggtat tccattaagc aagcgagagg tcattttcga ccgtggtcaa cgggttgata
     7801 ctttacgccc tgggcaaggt gtagggctgg cggtagcccg cgaaatcacc gagcaatatg
     7861 agggtaaaat cgtcgccgga gagagcatgc tgggcggtgc gcggatggag gtgatttttg
     7921 gtcgccagca ttctgcgccg aaagatgaat aaatatgtcc ttttaccact acatcaagtc
     7981 attgattcga tcaaacaatt ttcattgaaa aagtgccaaa aatcgcaaat tgactacacc
     8041 attaactaca ccgttcggtg cactgtatga aacaacgtgg aacaaataga cacaagaaat
     8101 atacaggcgg gtaatctttc cagggggaag cgcaccaatt catgaggggc gttaatgtcg
     8161 atatggggat ccccataacg gggctaccgg attttttttc cggttaacct ttaatcaggc
     8221 tggtgggttt tacataccct tgatcaccgt aatgatgatc cagatgtgca ccttttccac
     8281 caatggcgga aaagctccaa ccgtaacgat gatcgctatg gttgcctcga tggtctgcgt
     8341 ggtcattatg accgttcagc cagtgacctt aatcagaatt tttcgctaaa aaatgacgtt
     8401 ggccacgtca tccggaaagc acaaaatcac gtcgattttt aatgaacggt taaaccgtct
     8461 ttttgtcacg gtgtgtcact ggttgtcact ctccgtcacg attgaggtgg tggtcattct
     8521 gcccgaccgc gcatttttgc gctatcggga atatcaaaca gttacctcgc cgacttcctc
     8581 aaattgaggt ttccgaaaat atcagagcgg gttagcgatt aagctgacga gtaattaaac
     8641 tgcgaaaatt tcgtagtttc tgaatatatt ccggcctgct ggttaacagt cctcagatgt
     8701 gagggatgta gccagcgcaa ttttgcgctt tgctttaatc tcagtgagtt accgccgtaa
     8761 ccgctccggc ttcttccagt ggtacgtgat tttctccttc tcccgataca gttcaacgcg
     8821 gcgattgtag gccagcattt ccagaacgcg gatccgtatg tcgcgcatat ccgcatcatt
     8881 aagctggata ccatcacggc gcatcacctc agcaacaaca cgcgcataat tttcggctgt
     8941 cacgctgtcc ggctgcgtgg tctgttcgtc agcctgctgg ctgattccag agacgcggcg
     9001 gattaatcgc agtatttcgg cttctgtcat gctgctgacc tcattacacc ccgctaaatt
     9061 cttccagttt ctggcggtgg ctgtcgctta tatcaaaagc aaaatcctca tgctctgcct
     9121 ggaatgtacc aaacgccatc agcgccgcta cgctcgggtc tatcttgttc ggtgattttt
     9181 tcttgttcgg cttgatattg gcgttcgcgt cactctgcat cacgacgtta ctcatcgacc
     9241 aggacaacac cggatcgcca cgatgcacaa tcaccctgcg gttaacaaaa acttcgaacg
     9301 atttcgccgc cggactgaat ctgaggtagg tttgcgggaa cggctccacc tcaaaaccag
     9361 ccccctgtaa ttgcgtcctg agatgcgtgg cgttccacgt atcaaagccc accagcctga
     9421 tattaaattt ctccgcatcc tgcatgatgt catctctgat ccggtcgtaa tcaatgcagt
     9481 cacctggcgt tgtgcgtatc cagcccgctt tagcccactg gcgatagaca gcgcggtttt
     9541 tattggcggg gttctgtagc tggaactccg gcagataatg acgggaaatc agcataatgg
     9601 ttttaccgac cggaaaggca tagcatacgc tggaaatatc gctggttgat gataagtcca
     9661 gccccgcgta acactcctga ccgtgtaaat cttcctccgt gaacgttccg gcacactcag
     9721 cccatgcacc attacccatc cacggggtag tcccctgaca ccagatatta aatcgctttg
     9781 tcatcatctc cacccactgc gacggaatac cccgcgcttt ctggatggtt gaggccagtt
     9841 tttcacgatc cacggaaaca tcaatattag ggttcgcctt tatccacatc gccggatcat
     9901 caacctcgtt ttcgtcgtcc agttcgtaaa tcagtacaaa aattgaatcg ttgacctctt
     9961 cgccgtccag gatctggcag caataatcat agtgctgttt acaggctgaa acgacgttac
    10021 tgcccgatgt ggtaatggca aataacagcc cttcgggacg tgcgcccatc cccagctcaa
    10081 gcgcggaata aacgccgtta tcggggtgta ggtggtattc gtccacgatg gaaagactgg
    10141 ggtttgtccc ctcgatggtt gccgcttttg ctgccagcgg ctttaacagg ctgttgcttt
    10201 tcgggtgtat caccttatgc gcctgaatat tcacccgcct gcgtaacggt cgggataaaa
    10261 ggcacatctg acgcgcatta tcaaacacga tccgcgcctg gtcacggctc accgctgcgg
    10321 tgtagatatc ctgctgaccg ttctccataa tcagaaacca gttagccaga atcgcggcgg
    10381 tcgtggattt cgcatttttg cgcggcactt cgataaaggc gctcgtgtat ttgcgccgtc
    10441 cggtggcctt aaccttaaag ccgaggatgc acgcaaaggc gaactgctgc cacggctcca
    10501 gctcaatggg tctgccacgc atcggtcctt ttacgtgcgg gcacaccctg gaaaaggcaa
    10561 taaaccgctc cacaacctca cgatcgaacg tgtaaagggg gcttttaagg tccgaaaagt
    10621 accgtttaac agcctgtttt aaccgtttac aggccggaat tttgcccgtt tttacgtctt
    10681 ctgcgtactt attccaggcg gtcaagctcg tcctcttctt ctgtttccgg tggattttta
    10741 cggcggctta tcgggtcaaa accgagcaag gaggccattt ttatcatcac tctttcagcg
    10801 tcggattttg cgcttaatgc ggggtttctg ctctcgccgc cctgactgtt aacaatgctg
    10861 aacccgcgcg ccgcaaggtc tgcaacggct ttccggtaaa tggagtagtt gacgcaatac
    10921 agttccagat tgctccagtc ggcgggggtc aggtctcccc gttccgcaag ctgccgcgat
    10981 ttttcccgcc actgcttcac ggcgatatca tccaggtagg cggggtcggg tttgtcctga
    11041 aaacgccccc caccccgtca tacagcctca tcagcgattg cggaaacatt ccataacctc
    11101 gcggtcacgg tcggttaatc gcttcgctgt gatgcgttct gcgcgtcctg acgctttatc
    11161 tttatgccct gtttcctgtg tcttccatgc gtcacgctgc cttataagtc cacggataag
    11221 gcggttttgt tcccgctcat tcatcagcgt catacatcca gttattgcgg ttagcagccc
    11281 gttcttcctc ttcacgaaat ccacctgcgg cacgcttgct ttttgttgcc ggatcaagcc
    11341 atttcgtttt ctggttatga cacgcctggc acaatggttg atggttccat tcgggccaga
    11401 agagaacatc atcaccgcca ttaatcggga taatgtgatc caccaccacg gcgggcgtat
    11461 atatcccctt ctcaaggcat cgcacgcata acgggttttt actcagatac atggcgcggt
    11521 atttgtccca ctgtctggaa tatccacgcg cgcggcggtg tcctcgtctg gcatcctctg
    11581 cacgccatgc cgcccgcctg tgctcctcac acttgccgga tttaacgcgc ctgttacagc
    11641 ctggttctgt gcatcgtctt aatggttgcc acggcatcag taccccccca catcacggta
    11701 agccgtccag agtgcgccta tcgtcatggg tacgcgtgtt tttgcgttat ccgcgacaat
    11761 ctggcgattt tcatacaggt gagcgataaa catcatgcag ccaatcttta tggctggcgt
    11821 gaacgccagc ccgtcatcaa agcgcctgcc tatgtgtatc tggcacgcct caagcgatgc
    11881 ggcaatgtat ccgctgatta actggtcttc atcgtcgcca tcgatgcggc aatggagttt
    11941 cacttcttcc agggtgataa gttcttctgt catttttccg cgccctcacg acaaagaatt
    12001 tcaaggcgtg tcctggcggc atccggcagc ggctgcccga tgatattcag tacacgcccc
    12061 gccagcggcc ccgtactgac ctttatccgg ctggtggcgt tgatgtcctt ccggtaccgt
    12121 atccagatcc ttacggtacc aatcgccagc tctgcgcccg atgaaatggc ctccttgctg
    12181 ctgaccatgt tcacgcttgc ccagagtgtg tgaccgtcct cccacgtttc gagtatttcg
    12241 ccggttatat ccctggtctg tttcagggtc tgaatcgtta tcctgtcacg cagtcgccct
    12301 atgttcattc cggtttttct ccctcgctga tttttacttc ctgtttccat gcctggctga
    12361 actcatcacc accatcacgc ggtgacagcc cttcacgttc gcgggcttcg ttcggacaca
    12421 taacgtcgga ttttatgccg cgctcatagg tggcaaagcg ttcgccaggt gtggcccgta
    12481 acaggtccgc gctgtcaaac tccacctgat accggatacc aggtacaggc gaggccacca
    12541 gcaaggcgtt ttttatctgc tgctcaaagt tcgccagcca cgggcgcatc gtcatggtga
    12601 gaaatgcgcg gcttgcctcg ctgaagttgc tgtaggtgct gttgctgtat tcctgcaaaa
    12661 aaatcggcga gacgttgaac attcgggcga tgtcttcaat ggtgaagcga cgggaggcca
    12721 gccattcggc atcctgatta ctcatgccca gctgctgata gctcatcccc ccttcaagga
    12781 tgggcgtttt tccggcgttt ctggcccctt tgtagcgttc cagtgcggct aatgcctgtt
    12841 tgcccttcac gccgtccagc cattcgcctg acgtgataac ccctgccgcc atcatgccat
    12901 cacgcatcac gctcgcgccg tggcgttgtt gggccagccc aagccccagc gattcgcggc
    12961 agatggttac aggtgagcgc cccataaaac cgtcatccgt ggagtaacga aggtggagaa
    13021 cctcccacgg taaatagttg cgggtgtttc cggtgtaggc gtcagtgatg cagtagcgcc
    13081 agttatgatc acctgtctga tccacgttca ccgactgcgg cgggtaagga tgtaaagccg
    13141 ccggaaaacc atcacgcccc cactgaataa ccgcataagc attaccactt aacaggcagt
    13201 agcggatcat cattcgctta aactggtagg gtgtctgcca cgcgttcggg cgctcgttga
    13261 ggatgtgatc gaccggatga gaatcaagcc attcgcgggc ctccttcccc ttctcattgc
    13321 gtaccaggta caggtaacac ggcatggtag ccaccgcgtc agagatgacc gtgacggcgt
    13381 tcatgacggc ggggagtgat tccgctgtcc cagatgatac gtactcaccc gcccccgtgt
    13441 tcgatgtgcc ggccagcgcc ataaattcat caagcgtcat gctgcgctgc tctttttttc
    13501 gtctgaaagg ccacatatca cacccccgct aaatccgccc accagcggcg attatccgca
    13561 cgcggcattt tttcggggtg ctgctcatac agggaacggc gggccagctc cacgccggaa
    13621 tcggggtaag ccggtaccga tgtaacggta atttcgtaca gttccgccac cagcacggtg
    13681 cgcacgcatg gatctgttgt ggtatcccat acatccttac gggagcgaaa gccaaagctc
    13741 atccccgaga tgtccccacg cttaaccagt tcgataacgt cgcgccctgt actgggatcc
    13801 ggtggggtca gttcaaagcg taaccctgtt tcgtcctctt ccagtttcag cgtgccggaa
    13861 cgggtgcgcc ccagtaacat gctgtggtca tgctcataca ggccgcgaac gtcattaccc
    13921 gccgcaagcc attcagtaaa cgccccccgc tggaattttt cgtaaaactc accccataac
    13981 agttctgaaa gcttatccca gcgaacaacg tagcccgtca gcgtaccggc tccgctggtg
    14041 gtgatttccg atgaccggat ttccatactc ttcataattt tttcacccat aaaacactaa
    14101 aggggctttt tagccccttc tgtatgctgt taatcgtcgt cttccggcag tgccagaatc
    14161 ttgatcgcgt tcgaatccac cacgccaccg cctaaatatt tttgcgtgaa aattttgatg
    14221 aatcctggtt ctgtcaggtt gtccggtctg gtgcgaacac ctgtttcgtg atcaacaatg
    14281 tagtaaccgc gtttgaagtc acccagggca ataacgttat caggcataaa ctccagatat
    14341 tcgaccggaa ggcccagcaa cgtatcagga tcacccgcct gtaaacggtc gcgccagata
    14401 taatcaccgt tcgcgttctt caccttctga agttttgccg ccgtcgtgga gttaaccact
    14461 cagaccgcgt ttttgcggta ttttttacgt aatgcaaatt tcaggtcgat cagcgggtcc
    14521 gcagatgtcc acgccagaga ttcggaaggt ttaattacct gcaacgtacc aaaatcacgc
    14581 tctttgtcgt tcttctctgc acggggtacg gataaaaaac cttttgcttt tttgtcaccg
    14641 tcgcccacaa ccagatcgct ttcttcggtt tccgtgaagg tgtcgccaat ctcacccgtc
    14701 agccatgaaa ggatgtccac atcggaaaaa tccacgattt cctgtgtggt gcgcgggtac
    14761 gcatagaccg gatacagctt aatgctcacc tcgttaatct gcggggtgct ggtctgttcg
    14821 cgtgccttac cctcttcacc gtggttaacg gtcgcaccgc cagcggaaac aagctgctta
    14881 aactcgttgc tgctgatttt cttcacggta cagatgcgac gcatggtgga ttcatccgcc
    14941 agcattcgca tgatttcggt gttcagttca gggataacgg tataaccacc atcagcggga
    15001 acgcctgtac ttaatgcgcg ggtttcaccg gtcagaatgt agttgcgtag ttctgcgggg
    15061 tcagtggtct ggctgctttt acctggcttg ctgcgctctt cgtctgcaat ggcttcaagg
    15121 cgggaaatgt ctttatcgag ggattcagct ttagcgcgta attcgtcaaa ttttgtgccc
    15181 tcagcatcgt taagactgcg gttttctttt tcagcgttct ccagcatgtc gcgcatctga
    15241 tttttaatgg cggttttctg ctggcgtaat tcgattattc tctgcataaa aaaagtcctg
    15301 gggttaagta aggaactcca ggacgcggca aacactcaac cgtttttcat aaggaaatca
    15361 gtaatcgcac cgatcgtttt cccgcctggt aatgaatatt ggcgagcaca ttaacaggcg
    15421 ggaaagtggc cccagcgtcc tggcaccacg ggcgagaata atcatgattc agttcgggta
    15481 aaatatgccg atccgctcag tgaacaacgt ggaacaacca cgaacaaata atttacaaaa
    15541 aataacaaaa agccggattg ctccggctgt atgtgctgta tgggtgctca gtctctgatc
    15601 cctttcagaa attctatcaa tgcatctgtc tgttcaggat ttactgccag catttcaccg
    15661 gatagcgcgc aacgaacaaa accatgatga tccttttcaa tcagcgcccc cgtttccaga
    15721 aatgcgcggt aatcagtgat attcatcgtc tccatgttgt cagcatggta tttatcacgc
    15781 tgtttaagta tctcatcaaa tttcatcggc attgcgtttt cctctgttgt gtctgtttgt
    15841 ttcagatagt aactatgccg gaccgtgacg aaaagccagt agtgctccat accgtttcaa
    15901 atgacgcaaa aaaagccgga ttgctccggc tgcgtggtgt ggttctgata ttcctactct
    15961 gctaatttgc tcagccccat gtctgcgtaa gtgcgattta ctgcatttct caggtctgcg
    16021 tagttctccg ttggcggctc cggtgtgccc ttgctgctct gttttaccag cgggtcgccc
    16081 accagccgcc cgtaagctgc aatttgtgct gtcatattgc cacctcagtg aagcgatccg
    16141 gcattgttct ttttcatctc ttccatcatt ttttcagtga gcattacgca ctgaaacccc
    16201 atgtttttga tgtctacggg tttcatgcct ttgtcagtgg atatcacagg cagaatatcc
    16261 tgatacacat cgcggtcagg gtaactaagc acacaaagcg cccatgatgc cacgggtgag
    16321 gatccttcgt ctgtttcaac ccaccagcct ggtgtagctg cgcttatttg cagaatcgtg
    16381 atccgtccgt tgtgcttgta aaatttttca ccaaattttg gttcgctcat gattccacct
    16441 ctccggtttt aacgttgatt gttgttacct gttccgcttc ggcaatctcc cgctctgtca
    16501 gcgtggcaaa atttgccgcc gtcgtggtca tgaatgcgct tatcagttcg ggatgtgctt
    16561 tcgcgtatcc ttcccccgcg tggcggtcta tcgttctgat tgccaccttt aaagcgtgct
    16621 ctgtcatgtc taacgcgcga tatttcggtt ctgttctgtc tctgcgtttt ttgaatgatt
    16681 tattaaattt ccctgaagtg tgcatattta tttttacccc ctcgtttaaa aagttttgag
    16741 ttgtgcctcc ccttgtctac cttatctacc ttagtggccc tcatgccagt aatggcgctg
    16801 ctttcagcgg ggtagagtgc ttttatccac tatctacccc gtgtctacct ccctgtctga
    16861 ttcaggtaaa atcaggtaga gagggtagat agtgggtaga cagtaaaaaa aggctatcta
    16921 cctaacttaa tgcactgaat taaatgtatt tttctttact caggtagaca gggtagacag
    16981 caattacaaa aaattataaa aacgcgtcgc actcgtctgt tgttattgcg ttagtctgcg
    17041 ttactccctt aactttccgc gtaatatatt catgtccgta aactttcgcg gctggcttca
    17101 tagccttgcc aaagtcattt acgtttagcg gtttgctcct gcctgcgtac gccataaacg
    17161 ccagatagac gcggtaaagg ctgtttctgg tcgtgtactt cactgaatcg ccaccgccgc
    17221 ccatcatcag gccgcgtgct tcctccagaa aattcaggaa ctggcaaaac tcaataaccg
    17281 gatccgtctg ttgctttatt gccagagctt catcaccgtc acgctgttcc agtagtaaag
    17341 cccgtgcttt ctcagagtcg gtaaagttcg ccagcaatcg gcggataatg acagggattt
    17401 cagccgcaat cttttccggt agctccctgt ctttttcggc ctcactaacg atattgtcga
    17461 aacggaaaat cacgcgacga cgtgccacac ctccggcccg ttcggtgaat atcatcgggt
    17521 tattgttggt cgccagcacc accgccctga ttacagccgt gaaacgcttt tcgtatttcg
    17581 ggttaatttc cacggggtca ccgcccgtga ttttcttgat gcccgttcct tcgcctgtat
    17641 atttcggctg gtcagccagg acgataagac gactcccgac aacctgcgca cgtccaccag
    17701 catcatcaag cgatgtcatt tcagcgctta ccgtgttctg tttccctgcc agaaggatgg
    17761 ctatgtgtgt gaatgtactt ttaccgctcc cgccgtctcc ggtggcctca ataaacatct
    17821 gccagtcgta ccggttcgcc ataatcatgt acagcgcggc acatatacgc atcatcttgc
    17881 gctggtcttt tccggctgcg tgctcaagcc atttatgaaa gtttagcgcg ttatcgcgga
    17941 tgttctcccc tggtgctggt ggcgtgtact caatgccgtt gtgcgtggtg atccagttct
    18001 ccggcgtgtg cggggaaaat tcccccgttt tcaggtcaag cgcaccattg gcgaacggca
    18061 gcaaatcgcc ggacggctcg cccattggtt cggcaataac ttttaacgct tccacggcgt
    18121 tattgattac gcgcttgctg aaagtggccc tgtgctctga atagatcgcc accatttcgc
    18181 ggctaagttc cattgtgctg accggacacc ataccccgcc gcgccatacg tgaacgattt
    18241 cactttcctg atgcacacaa acgccatcaa agcgctcggc aagcagctgc gcgcgctcac
    18301 tgtctgccat ttgtgccagt tgcgtatttt tctgctccgg catggtaagc cctgcggcaa
    18361 tattttcacg ctcagcattc agatagcgcc gccagttttc acacttctgg acgtgcattc
    18421 cttcggggta aaaatttgcg tcctgaatat ctgccgccgc cagcttctga ccaatctttt
    18481 tggtctccac caaatccagt tctccggcct ggtacagcct tacgcgtttt tttccatccg
    18541 gaacaatttt cagtgcatca agttcggcaa gctgatttgg cccaagccac acaggcggca
    18601 cattatcgcc ggatgcgggg ccatcctgct cctgccattg tttcgcgtgc gaccaggcat
    18661 cactacccgc aaagataatt acctctgtgt ctttgtgttt tattccgcgt ggctgttttt
    18721 ttacgttcgg tgccagtttc attttttacc cctgaatacg ttaagcatct tttttatttc
    18781 ctgaatattg gcgcgtgctt tctccctgct ggttggttta ctgcggggcg ctgcctgtac
    18841 cagcgtgaaa tcacgccgga attgataaac aggcatcacg caatcatatt cgtaaccttc
    18901 acggcggtag gtgatgcgcc gttctgccac gcctttaatc gttaccatgc cgccgtactg
    18961 gtcgcggtaa atatcaccgc gcgtaaattt agggtgagtg ttgccactgg cagttaagcc
    19021 agaatattta agtttcatta tttttattct ccggtgtgtt gttctttata tctgtcgtgc
    19081 aatagatcta tttcttgcag ttccattatt acaggctcaa gaagcgttat taatgccgtg
    19141 gcaattcttg atttttgttt gtcgcgttca ttgtcgccaa gtgtttcaag ccatatgcgc
    19201 aatatttcca gcatgttttc actgtgagaa agtgcaagaa atgcgcggtc tattgtttcg
    19261 tggtaaatat cacgcatatt aatccccgtc cgtcgttttt cttaaaataa cctctgtcac
    19321 gaaatcagca taatcggcgg cgatattcag tacatcaagc ccgttgattt atattctctc
    19381 gtggaaagta agaaaaaagc cgctctaata agttctggca ttgacgaaag cgcatcagcc
    19441 gcatcatcag gaacgccgga aaattcctgt ttcagggaat taaaacgatc atcacgcata
    19501 acccccccca ttttcacaat cggcaacaag aatattttta gcgtcattca gtgaccgcgt
    19561 tgcgatgtat cggatacatt ccagggcgaa ctgtgtccgg tctttctgtt cttctccctg
    19621 cgcaagctct gccgtgcatt caatatcaat aagcgcgtgc atcagcgtag tgagtgcggc
    19681 ggctgctgcg ttcggtgtgg ttttattgtg cataggtttc cccctgacga atacgggcgg
    19741 cgaatacagc aacacaaccg gacgggcaac ggctacgcgc ttcgcgttcc gtccaagcgg
    19801 ttacgtggat gatttgggat tctccggcac tcagtgccag aaaacgccac acaaaggccg
    19861 tttgtgtgtg tacaaggtgt ggtatatgat ttacggcaac cataacggct cctagtttac
    19921 gttgttggtt agaagccccg ttactgctcc tacagtgcgg ggctttgctt tttgtgttga
    19981 ttgtgtgtaa tgtgtcacta cacataaata cattacctga ggtgtaatta acgtgtcaac
    20041 acacaaaaac gaaaggcgtg gtaatcctcc gttccagttc cgtttagatc ctgacttacg
    20101 ggaaaagatg gaaaaggcac aacaacagga cggcgatgat tctcttgctg cctggataaa
    20161 gcgaataatc cgcaaagaat tacagcaacg cggcatagaa cccaaaggat aaaaactatc
    20221 agcgccgtgg tgtgaggtac tacggcgcat tgctttacag gcgcacacaa tgaccaacga
    20281 agaatcaacc gaaaaaacat cacctgcacg aaaaagacga cgcagaaaga aagagcatga
    20341 atcagaacga ttcgcgccgt gttcttttgc ccttgagaag taccttaaag aatactcatc
    20401 aaaggagaga acttcgcagt tatggcaacg cactgaacca gactgattgc attgcccacc
    20461 agcctgatag cggctatcat ccccgtgctt atgtttggga tcacacacac aaacggcgca
    20521 gcgggttatc tgttcagaaa ggcggctcca tttcggggcc gcttttttta tgcctgaaaa
    20581 accccaattt tgtggttttc cagtttcacc agggcgaacg aatccccgcc cacgttctgg
    20641 cgtatattca atcttcatgg ttatagctct gtgttcagat gattgatgtg tggcggctgc
    20701 gtgccgccag cgtgattaat gaactgcctt gcagctatcc ttccaggcca gaacctcgga
    20761 taaagaccag ccaacggaac gaccgccaag tttacgacgt gatgggaatt gtccggcctt
    20821 ttccaggcgg tagcggcatg agcggctaag gcctgttagc ttttcgcatt ctttttcacg
    20881 tataaaccga tcagtgctta acactattgc cccctttcgt ttcttaaaga gttatttcgt
    20941 gttctattgc gttgggatgt gtctgattgt gtcaggatga ttcagagttg gcaaatgttg
    21001 aggccgtatg gtttacagaa agaggaataa tcaggataaa atcctttaaa ttcatgttaa
    21061 tacaaaggca taaaatattg tttcatgcct tttttctcgc tatttaaaga gtgattcgct
    21121 agtgtataaa aaatcagtaa cacattaaaa atcagctact tataaatctg cacacttttt
    21181 cacctcttgt tcgtagttgt tccgctttgt tgctcattgt tgcacgttgt atctgttcgc
    21241 atatccagta tgcgcatact gaaaaaacac gaaaaaaatt attttcttct ggctactggt
    21301 agcgtggtta cgttttcatg tgttcccgcc agtatcccta accgctccgt ccacatatcc
    21361 agcgcattgc gtttagcatc cagataacgg gaatgattat aaactcgctg cactcctggc
    21421 atctggtggc ctgtaagctg ctccacgacg tgaggatcca cgcctaaatc gttcagcatc
    21481 gttgtaaagg tgcgccggat gtcatgtagt gaccagtgag ggtgatttag cctcctgtgc
    21541 gctaatctgc cgtactgcga cacgcttgtt tcctgtttca cttcccccag caataagccc
    21601 gtgtgcctgt tctgctccac cagctgcgtg acgaacggca gtattgcttc cggtatgggc
    21661 cggaatattg ccaccttcgt tttgctgtgt tctttcggca cggtccagag catttcggta
    21721 aaatcccact cgctgatctc cgataacctc agttctaccg tccggcatcc gaacacaatc
    21781 aggaggcgga ttaacgcgat gtagtagggg gagaatattt ttttgtccag tgcctgcaat
    21841 aattcgccca gttctttggt gcttaagaca cgctcgctta tatccggttt tttcccaacg
    21901 tccgccacac tcatatcatc aagaacgttg ctgattgcat agcgccgcct ccggcagaac
    21961 ttaagcgcct gtttgcacgt ctgtagcaag aatccggcag taacaggcgt tcgctttgcc
    22021 acctggtcaa aacaggccag ccagtgccgt agctcgcatt tatccagcgg catagcacca
    22081 atgtgctgta ttacgtgatt attaaggcgc tttttcaggg cggcataatc cacgcggttt
    22141 tcctttgcgt acgactcaag ccagtaggtg agcgcatcgc caaccgttac cggctttaac
    22201 gcttcctgta cggtgtaatt aagctcatga cgtggatttt tcccctctgc cagccatgcg
    22261 cgacactggg cggctttttc cctggctgat ttcaggttca gatcaggata atttcccagc
    22321 ttaatgcgtt ccggtggtgc cccccttccc gttccggccc tgtaagtgaa ataccaggtt
    22381 aaaaggccac tggttgaatg cctgacgctc aggtttccac cgtcattaag aaaggctgtt
    22441 ttttgggcgg gtgtgccgtt gattttcctc agctgtgtat cgctcagttt gttaagtgct
    22501 ctgctcataa ttttgattcc ggtcatcaca attaactaca ccattaacta caccgatcgt
    22561 tgcacaaagg gctacaacgt gaaacaagct ggaacaagga aaatcacaaa tctgtttata
    22621 atcaaaaaca taataaacaa tctgaaacat tatgaaacgg caaaaaacac taattgataa
    22681 aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg cagataccag
    22741 cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt ttcttgaacg
    22801 tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta ttgacccgat
    22861 ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc gactggtcag
    22921 tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg atcatctcgg
    22981 tgaaaccaac tggtcattac tggtacaggc agtaaaccac tggcatgagc cgaccgccgc
    23041 gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga tgatttcttt
    23101 ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt ttatcattca
    23161 gggtaccgga cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac agcactgccc
    23221 acatccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag agctggagcc
    23281 tggtgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc tggaaaatgc
    23341 gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta gcggatttgc
    23401 cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg atgttccacc
    23461 tcgtgctcat cctgcggatg ttctgccgca agagatggat aaactgcgtg agatgatgct
    23521 cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta tatcccagtc
    23581 acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg aaatctacga
    23641 tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat tgcgcattgg
    23701 cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg cactggatgc
    23761 actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg aagatccgtc
    23821 attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg aagggtaagt
    23881 ttggatttaa gccggatgca gcatcacatg ccgcatccct tttcgcttac ttccgttgcg
    23941 ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct tccagaatca
    24001 caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg caaggtaatc
    24061 ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt tccggttcaa
    24121 tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc tcaaccactt
    24181 tctcgcgcat attgtagtaa ctgtcttcaa tcaccaattc aatgtagcaa tcaggatgta
    24241 accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct tcaaactgtt
    24301 tacggtcgaa atcacggatg atgtagtgca tatcggcccg ttcaacggtg cctttcatgc
    24361 tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca tccgccggaa
    24421 cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct ttcgccgtgc
    24481 ccggatgaac attgttaccg acaattttta tattgaccga tgcggcgttg aagttttcaa
    24541 actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca tcgaaggcat
    24601 caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag gcgacgcgaa
    24661 tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg atttctgcaa
    24721 tacctgcttt gtcatcagca cctaacaagg ttttaccatc ggtggtaatc aacgtctgac
    24781 ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg ccgataccca
    24841 gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attaacattt ttgccgctgc
    24901 aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg ccagggacgt
    24961 tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg ataagcccca
    25021 tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc gtgctgggaa
    25081 cctgtctcac ccctgctttt gattgggtat ccagagacac gtagttcaaa aatcgctcaa
    25141 gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga agcaagaaaa
    25201 gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat cagcattacc
    25261 ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc aagcggtttg
    25321 ccgtagaatt accgccctca ttaagagtca ccaaggtggt taaccacaaa ccccgcatcg
    25381 gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac aaccgagttc
    25441 gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta aagaggtcat
    25501 tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg gcccttctgg
    25561 ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg attccggacg
    25621 catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct atgtgaacac
    25681 tgttttccaa agctatgcac ttttccccca catgaccgtg ttcgaaaatg tggcctttgg
    25741 gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg aagccctgcg
    25801 gatggtgcag ctggaaacct tcgctcaacg taaaccgcat cagctctctg gtggtcaaca
    25861 gcaacgcgtc gccattgctc gcgcggtggt taacaagcct cgtctgttgt tgctggatga
    25921 gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc tgaaagcgtt
    25981 acagcgtaag cttggcatta cattcgtttt cgtgactcac gaccaggaag aagcactcac
    26041 catgtcggac aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg gcacgccgcg
    26101 tgaaatctac gaagagccga aaaacctgtt tgttgccggg ttcattggcg aaattaatat
    26161 gtttaacgcc acggtgatcg agcgactcga cgagcagcgc gtacgcgcca acgttgaagg
    26221 ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac tgcatgttct
    26281 gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg ctgaagggct
    26341 gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg ttgttgaact
    26401 ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgatc ctgactttga
    26461 ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg tcgtactggc
    26521 tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc ggttggcttg
    26581 tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg acccgcgacg
    26641 acgccagttt cgtcaaaatg gtctttacgc tggataacta cacgcgtctg ctcgatccgc
    26701 tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc ctcgcctgcc
    26761 tggtgctggg ctatccattt gcctggtttc tggcgaagct gccacacaag gtgcgtccgc
    26821 tgctgctgtt tctgctgatt gttccgttct ggaccaactc attgattcgt atctacgggc
    26881 tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg ctgggcgtta
    26941 tcgacacacc aatccgtatc atgttcacgc ccagtgcggt gattatcggt ctggtttaca
    27001 ttctgctgcc gtttatggtg atgccgctgt actccagtat cgaaaaactg gataagccgt
    27061 tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc cgcatcatta
    27121 ttccactgac gatgccgggg attattgccg gatgtctgct ggtgatgctg ccagcgatgg
    27181 gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc ggtaacgtca
    27241 tcaaggtaca gttcctcaat attcgtgact ggccgtttgg tgcagccacc agcattacgc
    27301 tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt ttgctgaata
    27361 agaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga ccgctatcta
    27421 cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca gctcgcgctt
    27481 tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga acaacgacag
    27541 cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga cgtttgctac
    27601 gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgcg gtaagccgtt
    27661 cgttagcgga atgctgtttg tggtaatgat gtcaccagat atcgtgatgg caatttcgct
    27721 gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc tgttctcgca
    27781 tatcaccttc tgcctgccat ttgtggtggt gacggtgtat tcgcgcctga aaggttttga
    27841 cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc gaatttacca ttctgcgaaa
    27901 aatcattctg ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa gctttaccct
    27961 gtcgatggac gatgtggtag tttcttcatt cgtcaccgga ccaagttatg aaattctgcc
    28021 gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg cgctggcaac
    28081 catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg ctcgtgataa
    28141 aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct gctcgcggcg
    28201 ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac gctgtatttc
    28261 tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa agaaaccggt
    28321 attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtacgccaa gctgaaaacg
    28381 tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga taaaatgcgt
    28441 aaagagggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag caatctcgat
    28501 ccagacatgc tcaacaagcc ttttgacccg aataacgact actccattcc gtatatctgg
    28561 ggtgcgacgg cgattggtgt taacggtgat gcggtggatc cgaaatctgt caccagctgg
    28621 gccgatctgt ggaagccaga gtacaaaggc agcctgctgt tgaccgacga tgcccgtgaa
    28681 gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga tccgaaagag
    28741 attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtcgcagc gtttaactcc
    28801 gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat ctggaacggt
    28861 tctgctttcg ttgcacgcca ggcgggtacg cctattgacg tggtgtggcc gaaagaaggc
    28921 ggcatattct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa agaaggcgcg
    28981 ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc tgaaactatc
    29041 ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gtccagaagt ggcaaacgat
    29101 aagacgctct acccggatgc tgaaaccatt aaaaatggcg aatggcagaa tgacgttggc
    29161 gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg ttaattcagc
    29221 aagcaggcgg tggtattacc gcccgttttt tagttagcaa ggatgtggct aatgagtcag
    29281 gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg tccgcttgta ggcctgataa
    29341 gcgtagcgca tcaggcaatg cttcctgctt tataatccct ccagcaactt ttcaacaaat
    29401 tctggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt accaacctga
    29461 ctcggttcaa gattcagttc aacggtgtga gcaccatgta gtttggcttc gtgtacaaac
    29521 ccagccgccg gatagacatg cccggaagta ccaatggcaa taaagacatc cgccatcgac
    29581 aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac gacgtgcggg
    29641 cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac gtctccggtc
    29701 cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc atgcatatga
    29761 atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt taccagcaaa
    29821 aagcgatcgc cgagagcgtc ttgcagttta gccagcgcaa gatgcgcggc gttaggctga
    29881 atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac cagttcagga
    29941 tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc ccacaggcca
    30001 tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc tgtcagtacg
    30061 agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa aatacgctga
    30121 cgcaaacgct cgcgcaggcg acgtttattt ttacgaaaac gacttaaccg atgaccccga
    30181 cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc
    30241 attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag
    30301 agatgacaag gcaacctgtc cgccagttgc gttgtgattg ccgggaaatt cgataagcca
    30361 ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag agaaaccgct
    30421 aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg atcataaagc
    30481 gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag ccacgcaaaa
    30541 ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca gcggcgtaac
    30601 gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg gccaaactcg
    30661 ccggtaatgt agcttttacc agtaatcggt ttgccgttga aaaccagccc gccgccaacg
    30721 ccggtgccga gaattaaccc catcaccagt ggatattgcg taaattcatc atcccaggct
    30781 tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg
    30841 ctcaggtcgg cacgcagcgg tttgccgctg gcagcgggga cattagcggc atacagctta
    30901 ccatcttctg tttccggcat tcccggaata ccgatgccga cagaaccttt acagccaaaa
    30961 cgttggtcag cttcagctac cagctcacac actgcatcta aaaatgcgtc atagctgtca
    31021 cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa cacgccaagc
    31081 gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg ttttaatgta
    31141 ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc gccgcgccgg
    31201 ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga agacgtccag
    31261 ccagtgcaat tccgatggca ggaagtcaat aaaataaata tcgctggaga ggaactgatg
    31321 accgatcagc ttttcaatcc actcaataat cggggtaagt tgcagtgaaa caactacgcc
    31381 gataaccacc ccacacaggc tgccgaacag ccctgccagc aatccatacc agacaaagat
    31441 ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat cgccactctt
    31501 gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc caatcaccag
    31561 taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca tatagccgta
    31621 agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg catcgcgtac
    31681 cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca cgctggaacc
    31741 catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt gatcgagttg
    31801 accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct gcatcagttt
    31861 atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct tcactttcag
    31921 cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa aattgcgcca
    31981 cgcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt gtgggttaac
    32041 gcccttcacc tggattgcgc gcagattcgc tccactttcc accagaccgg tgaaattgat
    32101 atacggcgca gcggcggcaa tacccggcac tttctgcacg ttatccagtg cttcctgcca
    32161 gttagtccat ggctgattta ccgcttcgat ttcgccatgc ggcaccaccg ccagaatgcg
    32221 gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca ataccgccac
    32281 gccgagggca atgccaatgg tagaaatgac agagattagc gacaccatgc cgccgcgtcg
    32341 ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca ttactccgcc
    32401 cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccagttg gcggctcata
    32461 cgtttcgcca gttgcaggtc gtgagtgacc accaggaaag cggtgccctg caagcgattc
    32521 aactccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt accggtaggt
    32581 tcatccgcca gtaccagacg cggattattg accagcgcac gggcaatcgc tacacgctgg
    32641 cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag ccccaccgct
    32701 tttaacatct ccagtgcaca gctgttgatt tcagcgggct ttttcttgcc aatcagcagt
    32761 ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa ctgataaata
    32821 aagcccagct tctggttgcg cagttctgct ttcgccgctg aagacagttt gctcatcggc
    32881 tgaccgttaa agatcacatc gccggaggtt ggcgtatcca gcccgcctaa caggtgcagc
    32941 aaggtacttt taccggaacc agagctaccg acaattgcca tcatttcacc ttcgcccacg
    33001 ctgaaactga cattgagcag cacatcggtt tgcacactgc cttcctgata gcgtttgcac
    33061 aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg gttgtgtggc
    33121 agcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc
    33181 aataacaatg acctgtaaag gttcgatagc caccggcagc gccgcgccat caagcaggac
    33241 gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa gcgccgctcc
    33301 gaggattgca ccgataatcc cggcgctagc cccttgcacc ataaagacca tcatgatttg
    33361 tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct ccatcaccat
    33421 cagccccagc gaggtaataa tgttaaacgc cgcaacagcg acaatcaggc tcagcagtaa
    33481 gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac ggtcacgcca
    33541 gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa ctctcagcgg
    33601 ctcatccagc cacaaacgcc agccggtaat attggctgcc ggataacgca tcagacgcga
    33661 ggcatcctca atattcacca gcatttcata gccatcgact tcactgttgg cggcgaaagt
    33721 gccaatcaca ttgaacaggc gctgacttgg aatacgcccc atcggcgtga actggctggc
    33781 agatggcacc atcacgcgga tttgatcacc gcgattgacg cccaactgtg aggcaagttg
    33841 ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca cattgaccag
    33901 atacggcatt agtggatctt tttgcgccgg gtcgataccg agcatcaccc cgaccgccac
    33961 gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc gattaacgcc
    34021 gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat gctcagaaga
    34081 gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct caaagccgtt
    34141 catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga tggtagaaag
    34201 ccaggagacg aaacgaccga agcgatccac tgcacgccca cgcatgtaac gcaggccaat
    34261 aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa gacacgagta
    34321 tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg
    34381 aaaagccccc gtaaatcaat cggctaaatt gccatgtttt atcatcaatc ttgccgcctg
    34441 atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg ggtcataacc
    34501 agcaggatca accgcatgta aattaaaaaa gagcgtgttg ttctgatgaa acaaaaagag
    34561 ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta tcactcggtc
    34621 attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt cctgagcaaa
    34681 tgctggatct atttcaatct ttaccttgcc ccctttcgta tgccggtttt tttctttata
    34741 tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg tctcgataaa
    34801 cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg gctggcgtta
    34861 agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc caatgccgct
    34921 tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac cagcttgtgg
    34981 tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg tctggcgctg
    35041 ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc cacgccgtgg
    35101 tggggaatga acagtgtgat acgcaatttg ccttattaca gccttggcgc atggtttggc
    35161 gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct gatggcttct
    35221 ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg
    35281 gtatcgattg tggtgatcat gaagctgttt tatcagtacg agcaacgatt cggtatgcgc
    35341 tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac cacccatcgc
    35401 attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg
    35461 ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt catctatact
    35521 gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct gttgttctcc
    35581 ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcacccc agccggggcg
    35641 attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta tcagagatat
    35701 gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc ctgaacaata
    35761 tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc
    35821 agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg tggtactcat
    35881 tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt tcaccgatca
    35941 gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt cgcctcatca
    36001 ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc agcgtggcat
    36061 actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt ttctccacgg
    36121 tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac gaacccaact
    36181 ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat acgccacgcg
    36241 cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc ttgatttctt
    36301 tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca cgctggagga
    36361 agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag cggcaattga
    36421 actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg aacatattta
    36481 ccagcaagtg agtaaaggca cattacctgc tgggatcgag tactggcagc cattgttctt
    36541 cagcgaacca ctgccgccgc tgttcagtta tttccctgcc aataccttgc tcgtgaatac
    36601 tggcgatctg gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc gttttgagaa
    36661 tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc tgcgggtgga
    36721 cgagctcttc tcagagctga aaaactggcc ccgagtgcag ctaaaaactg aacatttacc
    36781 gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg ccgttcaggc
    36841 gcaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg acggtccggt
    36901 ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc tcgcacgaat
    36961 taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct
    37021 gatgattggc gctgccgaac atggttttgt cgataaggcg cgtaatctgg cgctgatctg
    37081 cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc gccgcaccat
    37141 caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc cggtggtcca
    37201 tctggagcac ggcgtcggtc gctatgccgg aatgaccacg ctggaagctg gcggcattac
    37261 tggcgagtac ctgatgctca cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc
    37321 actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc tgcataaact
    37381 tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc
    37441 ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca
    37501 cgatcgtgag cagtatcagt tattctgcga cagcttcccg tttgaaacca cgccggatca
    37561 ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa tggatcgtct
    37621 ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg ctttcctggc
    37681 agtagataac cacaagcagg tagcggtgtt ggtgcctacc acccttcttg cgcagcagca
    37741 ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa tgatctcccg
    37801 tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag ggaaaatcga
    37861 tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag atttaggcct
    37921 gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca ttaaagcgat
    37981 gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta cgctgaatat
    38041 ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc gtcgtctggc
    38101 agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga tcctgcgtga
    38161 aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca ttcagaaagc
    38221 cgccgaacgg ctggcagaac tggtgcctga agcacggatt gccatcggtc acgggcagat
    38281 gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt tcaacgtgct
    38341 ggtttgtacc accattatcg aaaccgggat cgacatcccg acagccaaca ccattatcat
    38401 tgaacgtgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc gcgtcggacg
    38461 ttcacatcat caggcatatg catggctgct gacgccgcat ccaaaagcga tgactaccga
    38521 tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggggcag gttttgcgct
    38581 ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag aacaaagcgg
    38641 ctcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc
    38701 actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa cagaagtcga
    38761 gctgcggatg ccgtcgctat tgccagatga tttcatccct gacgtgaaca cgcgtctgtc
    38821 gttctacaaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga
    38881 gcttatcgat cgcttcggcc tgctgccaga tccggcgcgt accctgctgg atattgcccg
    38941 tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg agaaaggcgg
    39001 ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg gtttgctgca
    39061 aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta ttcaggattt
    39121 gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac tggaagagaa
    39181 cgcgatcgct taatgtaaat cgggggcgtt tctgcgcccc catctgcaac atttacaaat
    39241 tctttgcact ttcctgcact atccgacact gtcaccatcc ataattcagg cttatctgtt
    39301 tattacaata accttatatt tattatgtat ttttggtgat gatcaaaacg cgtttttctc
    39361 gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg gcaaaagcca
    39421 acacctggcc gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg
    39481 tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg
    39541 ctctgttaca agctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa
    39601 cgatcccgtt gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg
    39661 cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag
    39721 gtattggtca gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca
    39781 aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg
    39841 gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc catcatgcga
    39901 ttcgtctggc ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca
    39961 ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct
    40021 ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg
    40081 ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg
    40141 acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag
    40201 cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg
    40261 atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc
    40321 gcgacacggc atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc
    40381 ttatttataa attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg
    40441 gaaagatttt gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag
    40501 atttgtcccc gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg
    40561 aacttcgaca gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag
    40621 gtttttacgt ttttcataat agtggcctta tgcagatgaa tgacgtcgca agaaattact
    40681 taacgatcga taactaaatg atagatgtga tctggatcac atacaagata ttttttataa
    40741 caatcattaa ttaatttaaa aatctttaaa tttcagtcaa atagaaagca attattttta
    40801 tgggatctgg actggtgaac ggaggtgatt atttttataa taacctttca ttcacagtca
    40861 ccaggtacaa cggcatcatg gcaactgact caacacaatg tgtaaaaaaa agccgtggcc
    40921 gcccaaaagt gttcgacagg gatgccgcgc ttgataaggc catgaaattg ttctggcaac
    40981 acggttatga agcgacttct cttgcggacc tcgtcgaagc aaccggagcc aaagcgccca
    41041 cgctatacgc ggaatttacc aacaaagagg ggttatttcg tgccgttctc gaccgctata
    41101 tcgatcgttt tgccgctaag catgaagcac agctgttttg tgaagagaaa agcgtggagt
    41161 ctgcgctggc tgactatttt gctgccatcg ccaactgctt taccagcaaa gacaccccgg
    41221 caggctgctt catgatcaac aactgcacca ccctctcccc agattcagga gatatagcca
    41281 atacgttgaa atcacgccat gcgatgcaag agcgcacttt gcagcagttt ttatgtcaac
    41341 gacaagcgcg cggggaaatc ccgacccact gtgacgtgac acatctggca gaattcctta
    41401 attgtattat tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg gaaaaactga
    41461 tgcagattgc cggaacgact ttgcgtttat ggcccgaact ggtgaaataa gtcgcaaaaa
    41521 aggcccctca gcctgagcga atgaggggcc aaattgcaga gtacagcctt gttatacttt
    41581 gttattcaaa atcagttgcc cgttactatc tagcgggatc tgcgtacccg gatcgcggtc
    41641 catgcggatt ttgccctgct gatcgccaat cttataggtc acatcataac cgagcatttt
    41701 ttctgacttg tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag tatcgctttc
    41761 ctggagatag ccctggatct ggttaccggc atatccacca cccagcgccc cacaacagtg
    41821 gcgacatctt taccgcgacc accaccaaac tgatgcccta tcacgccacc agcaacagcg
    41881 ccgagcaccg acccggtaat gcgattttca tcctgcaccg gtcgacgatg ggtcactgtg
    41941 acgttgcgac actcctgacg cggtgtttta accgtttcct tgattggggt tgcagaaaca
    42001 acctgagcgt attgcgggcc ccgttcaaac acgttcagac tggccactgc cgctacgccc
    42061 aacgcagctg cgacaccaat cccgataccc gccaacattg atttattcac gggaacacct
    42121 ccttctttgc agttatgcca atcatgcaag cgaaggagaa aagcagctat cagacagaag
    42181 atgaaaagcg cgggaatgac tggaaatact taaggattcg gaggagtctg aaggaatgcc
    42241 tgatgcgacg cttaacgcgt cttatcaggc ctaccgctcg taccatttgt ccagggcgga
    42301 taaggcgttt ataccacatc cgccagtgta cgtcgattaa tgcaacttca aacgcggacg
    42361 gataacgcgg ttaatactcc ccaccagcat cattaatccg gttttaaagt aaccatgcag
    42421 cgcaatctga tgcattcggt atagcgagat atatacaaag cgcgcaattc gtccttcaat
    42481 catcattgag ccgcgcgtca ggttacccat caggctacca acggtggaga agttcgacag
    42541 cgataccagc gaaccatgat ctttatactg ataatttttc agcggcttac cgttcatctg
    42601 cgccagaatg ttgttcattg cgcaagtcgc catctggtgt gcagcctgag cacgcggcgg
    42661 aacaaagccc ccttccggac gcgggcatga cgcgcagtcg ccaatagcgt aaatgtctgg
    42721 atcgcgagtg gtttgcagcg tcggttccac caccagctgg ttgatacggt tagtttcaag
    42781 accaccgata tctttcagga agtctggcgc tttgatcccg gctgcccata ccatcagatc
    42841 agcctcaata tattcgccat ctttagtgtg caggccgcct tcatcagcac tggtgaccat
    42901 ggtttgcgtc agcacgcgaa cgccaagttt cgttagctcg ttgtgggccg cagcagagat
    42961 acgtggcggt aatgcaggca aaatacgttc tcccgcttct accagcgtta cgttcagggc
    43021 ttcgttggtc aggcctttgt aaccgtagct gtgcagttgc ttgatcgcgt tgtgcaattc
    43081 agcggagagt tctacacccg tcgcgccgcc gccgacaatc gcaatgttca ctttgccatt
    43141 tgcgcccagg ttggcagagt atttcaggaa cagattcagc atctcctggt ggaagcgacg
    43201 cgcctggtgc gggttatcga ggaaaatgca gttctctttg acgcctggcg tattgaaatc
    43261 gttagaggtg ctacccagcg ccatgaccag ggtgtcatag gcgattttac gttccggaac
    43321 caacagttca cctttctcgt cgcgtagttc tgcaatagtg attgttttcg cttcacgatc
    43381 aatatcaatg acggaaccca actggaactg gaaaccatga ttgcgcgcat gggccagata
    43441 gctcaacgca tcgacgcctt catcaagcga gccagtcgcc acttcgtgca gcagtggttt
    43501 ccacaggtgg ctgtggttac gatcgaccag cgtaattttg gcttttttct tacgtcccag
    43561 cttatgcccc agctgtgttg ccatttccag cccaccagca ccgccgccga caatcacaat
    43621 ctttttcaat ggcgtagtca acgtgatccc cttaaattta ttaaccaatt gttaattaat
    43681 ggttattaac atagctctta attaacaaca ggttacagca atgaaaatgt tcttcgctat
    43741 tgagaataac atgaatggtg cattggtcat accaaaattg atgttaatca agttttgttg
    43801 cttaaaagat aggcaacttt accgggggag atcaaaaaat agagtttatt gtttattatt
    43861 tgagcaaatt cggaagggta atatagggtg aagcgtatat cgagggcgtt tggtcgtcct
    43921 gataagatgc gcagcatcgc atcaggcatt gatgctcgac tgtcggatgc ggcgtggacg
    43981 ccttatccga cttacagaat tcggagcgca tcaggtaccg gcaaggttac ctgatgcgac
    44041 gagggcattt acccgagggt tttgaacgct ttaatgcgct gtaaatgcgg ggagatattc
    44101 ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt aataatgatg caactcttca
    44161 gatgtccgct ggctgttaag tgcttcatca ttacgcgaca aaataaccag acaacgatcg
    44221 cgattcttct cgcggaagtt ggtcacacac ttagtggcaa tatcggcata ctcttccggg
    44281 cgatcaatct tgccttccat gttctcataa gggaacaaat tagggttgaa gatcacctgg
    44341 cggatatcgc aaagaaaacc aatccgttcc gcccagtatc cgcccaggcc aacgccgcaa
    44401 attagcggac gctcgtcaac gttcagttgc aacattttgt ccacttcttt aagcagatgc
    44461 tgcatatcat gtttcggatg ccgcgtactg tagcttatca agcgtacatc cgggtcaata
    44521 aactgcaatt gtaagacttt ctcgtggtta cccggactgt tagagtcaaa accgtgtaaa
    44581 tagataatca tcgcttcctc acataagcca gggttagtga cctgctttct cttcctgcca
    44641 gcgttcatgt aactgattca gacgggtgct gatcgctttc cagcgagccg agtccatcag
    44701 ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta acacgttctg ccttgatcgg
    44761 tgacagatta tctaacacgc tgacggcccc tttacgatta ttgcagacca ggatcatatc
    44821 gcaacccgca tccagtgatg cctgcccgcg ttcggcataa ctgcccataa tcgcggcacc
    44881 ttccatcgat aaatcgtcag agaaaatcac gccgtcaaaa cccagttcct gacgcaaaac
    44941 ggttttcagc cagtagggag aaccgctcgc cggacgcgga tcaacatcac tgtagatcac
    45001 atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt aaggaactga agaccgacat
    45061 atctttagcg cgaatctccg cttgtggacg tggatcgcac ggtgtttctt tgtgtgagtc
    45121 tgccgttact gcaccgtgtc ctgggaagtg tttcccggtc gttttcattc cggcttcatg
    45181 cataccatca ataaaccggc tggcaattgc cagggctttt tgtggatcgg catgataaga
    45241 acgctcgcca atcgccgcgc tgatatgccc gacatccagc actggcgcaa agctgatatc
    45301 aatatccata gcgatcattt cgctggccat cagccaaccc gcctcttgcg ccagtttgcc
    45361 gccctcttcc attcccaaca gcgcagcaaa ggattgtgct gccggtaagc gggtaaaacc
    45421 ttcgcgaaaa cgctgcacgc gtccaccttc ctgatctacc gccaccacca gatgattgcg
    45481 cgatgctgcg cggatctggc gcaccagttc acgtaactgg gcaggatcat gatagttacg
    45541 cgtaaagaga atcagccctc ccaccagcgg atgcgccagt atttcacgct cttccgcgtc
    45601 cagttcgtaa ccttcgacat ccaacattac tggacccaca ctgctctcct tattgtttta
    45661 ttaatagctg ccgccaggtg tcatcggcca gcctgataaa ttgttgatcg ccggtttgtc
    45721 gccagcggta ctcaaaccac cctcctttga gcatcagcag ccagggaaac catcgcctga
    45781 cctgacgcca taattgcgcc ggataaatct tcgcgcgagt ggcatagtca ttgaccaatt
    45841 gccggtgctg ttcagtattt tccacccaca ccgccgccag ttccagcgcg atatcaccat
    45901 ctccagcata ctcccagtcg atgagtttta accctgatgc gctatgcact aaatttccgg
    45961 cgtggacatc catatgcaat ggacttaagc gtaaaggtcg tggttcccgc gctttgcgca
    46021 gacgttttaa cattcgcagc caacccacag tccgccgcgc cggatcgctt tgctgccagt
    46081 acagttccag taagggcaac agcgttattc gccagccaaa acgtggttgt tgatgtagat
    46141 aatacagcaa gcctgccagt tcgttggtat ccggcaaata cgtttttacc gcgccgggca
    46201 gatagtcgac taccatccag tcacggagat ataaatgcgg cttcggtgca atgcttgcgg
    46261 gtagttgtga taaagcccga tactggcgca agaacgcgga ctgcggcgca tcaggatcgt
    46321 gcggctgacg cacaacaaaa cgctggccct gatgttcaat gagaaaacta ccgccactaa
    46381 gcccactatt aaacgtcgtg acgggatgaa actgcgggaa aaagcgcgac agcaattcgt
    46441 cgcgcgtgat gggattattg ctgcgaaacg gcacctttac ctgaccagat aatttcgccc
    46501 gtctgcacca gcatcagctg catttgtagg gtcggagcgt taacgttgcc agaggcgctg
    46561 gagtacagca cgtaatgagc gccgacattg cgggcaatgc ctatggcttt actacgggtg
    46621 cctaaactgt cctgcggcga caaacctaac tgttgcttag ccatcgacag ttgctgggcg
    46681 gaaaccaggg taaatttccc gttattagcc agcgcatttc gcagcgtttc ggtcgcttct
    46741 gcggcattca gcgaaccgtt agtacggttg ttaacgctat caaccagcag gacgctaccc
    46801 gcagtcaccc cgtcagcccc aagcatctta ctgaccatcg gctgcattgc gccattccag
    46861 tcataatggc gaatatgcgg cgcaggcggt gccgttcgat cttcgtgctc aattgggcct
    46921 ggctgctgcg ggatcgtcgg caccgagggc actgtgggga caggctgttg tggctcggct
    46981 ggttgttccg gcgctggttt cacttcttct accggtgcag gttcacgttg ccccacacac
    47041 ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc gactcatttt tgtcatcaag
    47101 attcacccct ttacaaatat agataaagtc tgactttgtg cgcccccaga aaattggcgc
    47161 tgccgtacag cgttaccgcc gaatgtgcgg gaatggtaac gctgcgtggc ctttccagag
    47221 gatgcatctc cagccctctg gcgtcatacc agtaaaaacg ataatgaacg gtaacgggtt
    47281 cttgcctttc gttataaagc gttgaggatg ctgaaggttg aatatcagac gtcgaaagga
    47341 cgggcttttc tgcactgatg cccgcagcca ataaagatga ctccatcacc agcgattgct
    47401 catcattcac cggaatttcc ggatgtgaac gacagcccac cagcagtaac aacaccagag
    47461 acaccagccc aaagcatcct tttctcatcg ttacagacct ttatgcgcca gcattggtcc
    47521 cagcggacgg ccacccaaca agtgcatatg gatgtggtaa acctcttgtc cgccatggcg
    47581 gttggtattc atgatcagac gatagccatc ttcggcaata ccttcttgct cagcaatttt
    47641 tgccgctacg gtgatcatgc gtcccagcgc ctgctcatgc tcagctgaca cgtcgttcac
    47701 agtcggaatg agaatattcg gaatgatcag aatatgcgtt ggcgcttgcg gcgaaatatc
    47761 gcgaaacgcc gttaccagat catcctggta gacgatatcg gaggggatct cacgacgaat
    47821 aattttgctg aatatagttt cttctgccac gacattttcc tttttcaaat agccatgcgt
    47881 tgcgccatgc tacgccgcgc cacactgcga gtatagagta tggtcgagtt aaacctgctc
    47941 tttcaatctt aactcacgat ttcttaagca aaaaatcgtt ttgctgccgt atatatcgcc
    48001 attattccca tttctgcctg cgaatgcgct gaaaagacat tctctgtttg gtttacacga
    48061 caaatatgaa tgcgtatatt tctcatttgc atttgcattt gcatttgcat ttgcagcaaa
    48121 attattcgca cactaaatat aaccgctcat caccctcctc cggcatgagc ctgacgacaa
    48181 cataaaccaa gagatttcaa atgctttcaa cacaatttaa cagggataat caatatcaag
    48241 tcatcaccaa accgtcacta cttgccggtt gcatagcact ggcactatta ccttctgccg
    48301 cttttgctgc accagccact gaagaaacgg tgattgttga gggttcagcc acagctccag
    48361 atgatggcga aaatgattac agcgtaacgt ctacctctgc gggtaccaaa atgcagatga
    48421 ctcaacgtga tattcctcag tcggtcacta ttgttagcca gcagcggatg gaagatcagc
    48481 agttacaaac gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa agtcaggcgg
    48541 attccgatcg tgctctttat tattcccgcg gattccagat cgataactat atggttgatg
    48601 gtatccccac ctattttgaa tcgcgctgga atctgggcga cgcactttct gatatggcac
    48661 tgtttgaacg cgtagaagta gtgcgtggcg cgacaggact catgaccggg acgggtaatc
    48721 catctgcggc aattaatatg gttcgaaaac acgcgacctg tcgtgaattt aaaggcgatg
    48781 tctcggcgga atacggtagc tggaacaaag aacggtatgt ggcggattta caaagcccac
    48841 tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat aacgactcat
    48901 ggctggaccg ctacaatagt gaaaagacct tcttctcggg cattgtcgat gctgatttag
    48961 gcgatcttac gacgctttca gccggttacg aatatcagcg cattgatgtt aatagcccaa
    49021 cctggggcgg tttaccgcgc tggaatactg atggcagcag caacagttac gatcgcgcac
    49081 gcagtaccgc acctgactgg gcgtacaacg ataaagagat caacaaggtc tttatgaccc
    49141 tgaagcagcg gtttgctgat acctggcaag cgacactgaa tgccacccac tctgaagtcg
    49201 aatttgacag caaaatgatg tatgtcgatg cctatgtaaa caaagcggat ggtatgctgg
    49261 ttgggccata cagtaattat ggacctggct ttgatcatgt cggcggcacc ggttggaaca
    49321 gtggcaaacg taaagttgat gcgctggatt tgttcgctga cggtagttat gaattgtttg
    49381 gtcgtcagca caatctaatg tttggtggca gttacagcaa acaaaacaat cgttcagttc
    49441 atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg gcaatttccc
    49501 acaagccgac tggtcatcac agagcctggc gcaggacgat accacacata tgaaatcgtt
    49561 atatgctgcc actcgtgtca cccttgccga tccgctgcat ctgatcctcg gcgcacgtta
    49621 taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca ccacgcctta
    49681 cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct atacctctat
    49741 tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa tcaccggtaa
    49801 caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca ccacgttagc
    49861 catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta tccccggcag
    49921 caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaagggg tggaatttga
    49981 actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc gctatcttgc
    50041 agaggataac gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg ttaaaatgtt
    50101 caccagctat cggttgcctg tcatgccaga gttgacagtc ggcggtggtg ttaactggca
    50161 aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag
    50221 ctacgcgctg gtggatctct tcacccgcta ccaggtgacg aaaaacttct cgttacaggg
    50281 gaacgtcaat aacctgttcg acaaaaccta cgataccaac atggaaggtt ctatcgtcta
    50341 cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa cacctgtaaa
    50401 aaaggcagcc atctggctgc cttagtctcc ccaacgtctt acggattagt ggttacggat
    50461 gtactcatcc atctcggttt tcaggttatc ggatttagta ccgaaaatcg cctgaacacc
    50521 agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccagcct gatccacttt
    50581 agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt
    50641 ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac ctgttgcttt
    50701 tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt taatcagcac
    50761 gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggta acagccacag
    50821 tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaga acgacgtacc
    50881 gtcacgcatc cccagaagaa tacagattgg gaatgccagg cctgccagaa tcgcgtggat
    50941 gatgtacagg atcggcgcaa cgaacatgaa gaagaactcg atcggctcgg tgataccggt
    51001 caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcac ggttttctgg
    51061 tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt tgaacaggaa
    51121 gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa tgtcgccgtg
    51181 gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag gtacgttcca
    51241 gatgtggtgc agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa tgccaaacgc
    51301 aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag aaccaatcgg
    51361 cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag aaatgatcgg
    51421 cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac ggtagaaacg
    51481 gttaaacatg tacgctgcaa tcgcaccgga gataatccct ccgagtacgc cagtatccgc
    51541 caagtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg caaccacggc
    51601 catggtttta accatgatgc cataggcaac aactgcggcc agcgcggata cgccatcgtt
    51661 attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg caaagacgga
    51721 accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga aattcgcgga
    51781 accgacgccc agcagaatac ctgcgatagg tagtacggat accggcagca tcagcgattt
    51841 accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg ctcctgagta
    51901 tgggtgcttt ttttacgttc tcacgcgtgg caagggggga gagcctcgcc gtgtacaggg
    51961 catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccaa tttgtttgac
    52021 ggctatcacg tttcaacttc agatcattgg agaagttgca tattttgaca aaacatatgt
    52081 cacaatattt caaacgcggt tattcaccgc ccaattttcg gcggtgaact ttactcgttt
    52141 tagccattaa ttacgagctt taaaaaaact cattcaacgg atggattgaa ggcgggaagc
    52201 gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt cttcaacggc
    52261 aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag gttgattctc
    52321 ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca gtttccacca gtaatcgatc
    52381 cagggggaca taacgcgcag catcgcgcag ttgctccgca ttacggaagg tcacaatgcc
    52441 ggaaaaggag atgtaaaatc cgagatccag taatttaccc gccgtttctc tgtcctctgt
    52501 aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt
    52561 atcggcacgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac gaccaatctg
    52621 gatatgatgg atgaaggact cttgctgacg tactttagtt tccggtgtgt aataataatc
    52681 cagcccggtt tcacccagcg ctacaacacc ctcttctgcc gccagacggc gtaaatcttc
    52741 tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata cgacgttgtc
    52801 acgttcgcct accagatccc gcatatgtaa ataacccggt aatgttgtgg cgactgccag
    52861 acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt ccttatgcaa
    52921 agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca tgatgtctct
    52981 cttaaagatg aggaaccggt agcacaacgc ccggttgcag gtaatgctca atacgcagta
    53041 aaagatcggt gatgagaagc tcgcggttga tgcctgtgac agacattaac tgttcacgaa
    53101 tgtggcaaac atcccccagt atagcctgca ggcgcgaggg agaaagatgg tttgccagtt
    53161 cggcgaccag gcccggcaca tcaacattgg tcacctgcgc agcgccatga tggcgtttta
    53221 gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc cggagcttgt tcatgattaa
    53281 gggccgccag cagcgaatac cagtcgcccg atggcacgct atatgccaac gcctgacaca
    53341 atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg ccaggcgaac
    53401 cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga cattgtcact tcgcgtaaaa
    53461 gccaagtcac ggcgtactgt tctggaggcg gcgcaaggta atgtaaccga caacgactac
    53521 gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag gtttctgctg
    53581 gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt aaggcagcat
    53641 cggttaccca gacaactttc gcaccaccta ggcgtgcgtg ctcattcagc ttttcggtga
    53701 cctcacgtac cgcatcaatg cccagcgtat ttttcccttt ttcgggagcc agggtgtagt
    53761 aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg caacttttgt
    53821 ggccctgcgg ttgttggcag agtaaataac ggctcagggc gtagattaaa gcatcatcgc
    53881 ccatgcccgg taacgcctga atgagtagcg catggtgacc tcttccggcc tgatagctgg
    53941 ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt ccaactcctt
    54001 cacccagtgg gtcacggtag tgcggattgc atccatcacg gcctccagcg gctgggtggc
    54061 atcaatggta tgaatacttt tatcttgtgc agccagttcc agatagcggg cgcgggtgcg
    54121 attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg cacgcgcgcg
    54181 ttttaagcca acttccgggg taacatcgag atagagcgtt aagtctgggc gaaaatcccc
    54241 gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc ctccgccctg
    54301 atacgcctga gtggaaagat cgtggcgatc gccaatcacc caggtgccgt tagccagcgc
    54361 aggtttgatg accgtttcta ccagttgaac gcgcgcggca taaaacatca gaacttcggc
    54421 tttatcggta atgacttcat cgcctaccga tttgatatcc agcaccaggc ttcttaactt
    54481 ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc atgtcgcgga tacccagttg
    54541 ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt ccagcccctc
    54601 aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa tcctgcacag
    54661 acttgttatg actggcaaga ttggtattaa acgtgtgacc acctttacca tcggccacaa
    54721 aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc gcccccggcg
    54781 tagctatcgc acccggcggc aaaccggtaa tggtataggt gttatacgct gtcggcgttt
    54841 ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa atcacggttg
    54901 ggtcggtctg caggcgcata ccaatgcgta aacggttgat aaataccgag gcaacctgat
    54961 cgcgttcact ggcaacggcg gtttcttttt cgataattga tgccatcgtc accagctgat
    55021 ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg accgctttca
    55081 ccattttctt gtgaactcgc ttgagtaacg cgacatcggt ggtattggcg gtatacatca
    55141 aggtgtctgg ccagaaccaa ccttcaatcc actccggatt ttccagttca agtgcctgcg
    55201 ctacggtggc gtacttatcg tcactcagcg tgtgcttgat atacggagcc tcacgcaatt
    55261 gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagcgggaac tgtgcttctt
    55321 taccgctttc caacaatttc agcatctcgc gcacggtcat ctgcggtgta aagcggtaag
    55381 tcccggcttt aaagtgagaa agatccggtt cgatacgcag cagccattga aaaacccgtg
    55441 ggcgattgat gatcttatca gcataaagct gttcaccgag cgccagacgt ccggtccctg
    55501 gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca agatggcgaa
    55561 ccttccagac gcccacacca gcggcgatac ccagtaccac cagcaataac aagataatca
    55621 ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat attcatataa
    55681 cgttgctgac gaaaaggtga catcgccaca ggcacgtacg ggcatcactg gcattaacgc
    55741 attacaaata accatctcat ctgcctgcaa cgcctcctcc agcgaggctt gcacttcgac
    55801 aagctgataa gaggattgtg ccagtaaacg gatacagaat tgtcgcataa tgccgttaac
    55861 acctgcctga tccagtcgcg gcgtataaac tacattgccc ttccgccaga acaaattagc
    55921 cgcacagcat tccgtaaccc acccttcgct gtcaaggacc agcgcctcat cagcgtttgt
    55981 ctgctcaaga tgagagcgaa tcaatacttg ctcaagccga ttaagatgtt taatacctgc
    56041 aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc aacgtcatcc cttcgttacg
    56101 caaacggtcg taatgtgcag gataagccgt aacggagaga atccgcgttg ctggtccgct
    56161 gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt tcagcacacc
    56221 attttgctgt tctgctgcca gcgttttcat ctcttgttca agctgaggcc agaagtcaca
    56281 ggaaatcatc aaccgctgac aagcatcctg tagtcgcagg atatgcgccg ataacaaact
    56341 gactttaccg tcgataactc tggcggtggt aaaacaacca tcaccaaact gcgttgcccg
    56401 atcgcttact gccagcgatt cctgcttata accgttaatt aagaacatac cggctcctta
    56461 tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat aaaagctaag
    56521 aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa tgacaactta gatcttttta
    56581 aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt gtattccatt
    56641 ccgctgacct gacgtgcttc atgcggtacg aaatccagat cgcaaccttc atccgggtta
    56701 tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga gtagatagat
    56761 tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga gcttaccaac
    56821 acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc tttatcgcca
    56881 gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc aatgcctgca
    56941 tcacgcagag catttgccat tgccagagct gcgcctgcgc cattttctgg cggtgacgtc
    57001 atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg
    57061 cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc gcccagtacg
    57121 aagccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc attgcgggta
    57181 gataatgcac gtgccgcgcc aaaaccacca acgcccagcg gcgtactggc tttctctgcg
    57241 ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc atggccaatg
    57301 ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg caggccatac
    57361 atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa gaatgggctg
    57421 atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag tccgaggccg
    57481 ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg
    57541 ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa ggcatccatc
    57601 ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac taagccagca
    57661 aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc actctgaccg
    57721 gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa catgcccagt
    57781 ccggtcacaa ctacacgacg cttagacacg tttgtcctcc aaggagggaa aaatgattct
    57841 agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg cctggagatg ttcacttacg
    57901 cctggtggcc gttgatgtaa tcaatggcag cctgaacggt ggtgattttc tcagcttctt
    57961 cgtccggaat ctcagtatca aactcttctt ccagagccat taccagctca acggtgtcaa
    58021 gagaatccgc gcccaggtct tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa
    58081 cgcccagctg ttcgccgata attttcttaa cgcgttcttc gatagtgctc atactcttaa
    58141 atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt
    58201 tgcaactaaa tcccggcagg tcttaccacg attttacgtt attttgaggc cttacccccc
    58261 aataacgcaa atcattttgc actaattatt actcataacc acgcagactg cgcgcaacat
    58321 gagcattttg tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca tgcaaagttt
    58381 cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct
    58441 gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat acccgcacgc tggtcatcgc
    58501 tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc aacaacgttt acagtaatac
    58561 cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc gatcaagccc gctttcgccg
    58621 cagcgtagtt ggcctgaccg ccatttccca tggtaccaac cacagaaccg atagtgataa
    58681 tacgaccatg acgctttttc atcatagcgc gcattaccgc ttttgacaga cggaaaacag
    58741 atgaaaggtt agtttcgata atatcgttcc actcttcatc tttcattcgc attaacaggt
    58801 tatcacgagt gataccggca ttattgacca ggatatccac ttcaccaaat tctgcgcgaa
    58861 ttttttccag aacagattcg atagatgccg ggtcggtcac attcaacatc agacctttgc
    58921 cgttggcacc taaataatca ctgatcgcct gagcgccatt ttcactggtc gcagtgccaa
    58981 taactttcgc gccacgggct gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg
    59041 caccggttac cagtgcgatt tttccttcaa aattcatgat tttcctcttt taaagctcga
    59101 gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc
    59161 gtttcgtcag gccagtaagc accttgcccg ggccgacttc atagagatgt tctacgcctt
    59221 gcgctgccat gtactcaaca gacttcgtcc actgaaccgg gttatacaac tgacgtacca
    59281 gtgcgtcacg gatggcatca ccattggttt cgcatttcac atcaacgtta ttcacaacag
    59341 aaactgttgg tgcgttaaag gtgattttcg ctaattctac tgccagtttg tcggctgctg
    59401 gtttcatcag cgcacagtga gacggtacgc tcactggtaa cggcagcgcg cgttttgcgc
    59461 cagcagcttt acaggcagcg ccagcacgct caaccgcttc tttatgaccg gcaataacca
    59521 cctgtcccgg agagttaaag tttaccggag aaacgacctg accttctgca gcttcttcac
    59581 acgctttcgc aataggcgca tcatccagac cgatgattgc cgccatagcg cccgtgcctt
    59641 ccggtacggc ttcttgcatg aacttaccgc gcatctcaac cagacgcacc gcatcagcga
    59701 aatcaatcac gccagcgcaa accagcgcgg agtattcccc caggctgtga ccggccatca
    59761 ttgccggtgc tttaccgccc tgctgctgcc atacgcgata cagcgcaaca gatgcagtca
    59821 acagcgctgg ctgggtttgc caggttttat tcagttcttc agctggcccc tgctgggtca
    59881 gcgcccacag gtcgtagccc agcgccgcag aagcttcagc aaacgtttct tcgacaattg
    59941 gatagctcgc cgccatatca gccagcattc caacggtttg agaaccctgt ccagggaaca
    60001 caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc gcagagcccc
    60061 aagtgaatcc accgccaaag gcttcaagca gaaccaactg ccccggctta atgcgcccgt
    60121 cgcgtacagc ttcatccagc gcgcacggga cagaggccgc agaggtatta ccgtggcgat
    60181 ccagcgtcac cacgacattg tccatcgaca taccgagttt tttcgccgtt gcactgataa
    60241 tacgcaggtt agcctgatgc ggaaccagcc agtccagttg agaacggtca agattattcg
    60301 ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg aagacttcgt
    60361 tgcccgccat cgtcagatga attgaattct ctggattcac gcggtcggcg tttggcagcg
    60421 tcagcaattc accataacta ccgtcggcat gcagatgggt ggaaatgatt cccggctctt
    60481 cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa aataataata gtcccacgat
    60541 ctgttggatc gcaggtgcgc gccagtacat cggaaccgac gaccagagca tacttcaccg
    60601 ccccagattt cacgtattga tcggctacgc ttaatgcata ggtgaaacct gcacaggctg
    60661 ctgcaacgtc aaatgccggg caacctttaa tgtccaacat gctttgaatc tgacaagctg
    60721 cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca atctggtctt
    60781 tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc aaagcccatg gttgaaacgg
    60841 tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt acgagtgaca atccactcgt
    60901 cagaggtgtc caccattttt tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc
    60961 tgccagtacc aataatcttc gtatacatgt acgctcagtc acttttcggt tatataccgt
    61021 cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc gcagagttcc gcttttgcca
    61081 ccgtccagca gctcaaaacc agctgggtat acagattcca ggcgagcggc aattcgctga
    61141 ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga
    61201 ttggctgcac catgactttt tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta
    61261 tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt
    61321 aataacagta gccaccacga ccgttttttc ccttcaccct gagatttcag cagagaaagg
    61381 aacatcctga caacaccttc catcgtcttt aacgtgacat ttcctgtaaa gccgtcacaa
    61441 accagcacat ctgtcttgcc agttaacaac tcattggctt caagatagcc gatataattg
    61501 atagaaggga ttgttttaag caccgctgag gcatcccgaa tactgtcaag accctttact
    61561 tcttcttcac caatattgag caacgccacg cgaggattgg gaatttccac cacctcttca
    61621 gccagaactg agcccataat ggcaaattgc accaacattg tgctgtcaca atcgacgttg
    61681 gcccctaaat cgaggaccac cgttttgccc ttttgctgat gtggtaatac cgtcaccagc
    61741 gccggacgct caatcccctc caggggcttg agtaataatt ttgccagccc catcagtgcc
    61801 ccggtattac cggcactgac acaggcttgc gctcgacctt ctttcaccag ctccagggcc
    61861 atacgcattg aactcccacg actggcgcgg atagcttgcg aaggccgggc atcactggcg
    61921 ataactgact gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc agctttagca
    61981 agtaatggcg tgatggcgtc gggattgccg actaaaagaa gagtgagttg cgaattagaa
    62041 ttcagtgcct gcaatgctgc aggcactgtc acggaagggc caaaatcccc tcccatgaca
    62101 tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt ctttccccag
    62161 ttgcctgggg aaaatcctca ctaagcttca tcacgcagac gcgtgattac ttagcgatga
    62221 ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt ttttcaccag
    62281 aagttttgtc tacagacagg ctggtgactg cggtcagcgc gtcatgggaa cgacgcatgc
    62341 cacgtttgga acgggttggt ttattctgtt gtacggccat ggaccttact cctcaattac
    62401 ggtttatccc cgatccaacg gggagcacca attacttacg ctttaagctg gctaatacgg
    62461 caaatgggtt tggcttttgc acttcttcag gcagttcacc aaagaccatg tccgcttcgg
    62521 acacttcaca gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg atgatttcat
    62581 cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc ggttcatacg
    62641 cttccggcag tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa tacgttgtgt
    62701 agacctgatg agtaaacggc ttcccgcaac gctgacactc gagcgttacc gtcaccttcg
    62761 catcgccgtt taacactgcg agacgttggt tatcgatagc gaacgacatg gagcattcca
    62821 catcactgtc cacactgact acggattcgg cgacgcgctc aacctgatca ggggtataga
    62881 taccctggta atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc aggggtaatt
    62941 ttaccttttg catagggcgc gcatattaac tttgtaacgt catagagtca aagaaaaagg
    63001 cagcctgtgg ttgccttttg ccaataattc gcacacattg cgggttactg ctttattttc
    63061 gttcagtgct gataacgaac atcaacctgc aaaacgaagc gcatagttta aaatggctat
    63121 catcaatacg ctatatctga tggaaaaaaa tatgcctaaa cttattttag cctccacctc
    63181 gccatggcgt cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg cagcacctga
    63241 ggtcgacgaa accccacgca gcgacgaatc accgcgacag ttggtgcttc gactggcaca
    63301 agaaaaagcg caatctctgg cgtcacgtta tccggatcat ttaattattg gttcagacca
    63361 ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta acggaagaaa atgcccgtct
    63421 gcaattacgc aaagccagcg gcaatatcgt caccttctat actggactgg cgttgtttaa
    63481 ttcggcgaat gggcatctac aaacagaagt ggagcctttt gacgtccatt tccgtcacct
    63541 aagcgaggcg gagattgata attacgtgcg taaggagcat cccctgcact gcgcgggtag
    63601 ctttaagagt gaaggatttg gcattacgct gtttgagcgt ttagagggac gtgaccctaa
    63661 cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg aagggaaaaa
    63721 cccgctgatg ggatagttgt ttgcgccgga tgcggttacg tcgcatccgg caatcggtgc
    63781 agacggcctg atgcggcgta aacgccttat caggccaacc gcccattatc aggcttatat
    63841 gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacct tcatccatcg
    63901 gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt
    63961 gcaggaacag acgatttaat cccgtgcctg cttcagtgag ctgtctgtca aattcacggt
    64021 caccgtagcg atcgtcaaag gcaatcggat gacccgcata ctgtgtatgc acacggatct
    64081 gatgagtacg tccggttacc gggctacaac gcaccagggt ggcaaatgca tagcgttctt
    64141 ccactttaaa gcgtgtttcc gacggtttgc cttcctgact gacacgcacg atacgttcgc
    64201 cgctttgcag aatatttttc aataacggcg cttgaacgct cttcacatgc gactgccact
    64261 gaccgcgcac cagcgccagg taatcttttt gcattccttt ttcacgtaat tgctcatgca
    64321 gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaagtg tcccggtcga
    64381 gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcgataa
    64441 cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta ttcagcacca
    64501 ggatatgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg
    64561 gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tgggatacgc acctcatcac
    64621 ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact tcacctttac
    64681 gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc aaaaagttat
    64741 cgatacgttg ccctgcttcg tcagcggtga tagcaacaat ttttacggat ggagtctctg
    64801 ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt tttctatgct
    64861 tatatttagt tttgcacctt attacttcac tacgtgatca ctttattgat ggttattaaa
    64921 ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca
    64981 tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc atccttgtta
    65041 aaacaagaaa ttttacggaa taacccattt tgcccgaccg atcatccacg cagcaatggc
    65101 gtaagacgta ttgatctttc aggcagttag cgggctgcgg gttgcagtcc ttaccggtag
    65161 atggaaatat ttctggagag taatacccag tctgtttctt tgataattgc gctgtttttc
    65221 cgcatgaaaa acgggcaacc gacactctgc gcctctttga gctgacgata accgtgaggt
    65281 tggcgacgcg actagacacg aggccatcgg ttcacacccg gaaaggcgtt actttgcccg
    65341 cagcttagtc gtcaatgtaa gaataatgag taagttacga tgaaaagaat gttaatcaac
    65401 gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct gtatgacctg
    65461 gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc
    65521 cgcattgaac cgagtctgga agctgctttt gttgattacg gcgctgaacg tcacggtttc
    65581 ctcccactaa aagaaattgc ccgcgaatat ttccctgcta actacagtgc tcatggtcgt
    65641 cccaacatta aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat cgataaagaa
    65701 gagcgcggca acaaaggcgc ggcattaacc acctttatca gtctggcggg tagctatctg
    65761 gttctgatgc caaataaccc gcgcgcaggt ggcatttctc gccgtatcga aggcgatgac
    65821 cgtaccgaat taaaagaagc actggcaagc cttgaactgc cggaaggcat ggggcttatc
    65881 gtgcgcaccg ctggcgtagg caaatctgct gaggcgctgc aatgggattt aagcttccgc
    65941 ctgaaacact gggaagccat caaaaaagcc gctgaaagcc gcccggctcc gttcctgatt
    66001 catcaggaga gcaacgtaat cgttcgcgcc ttccgcgatt atttacgtca ggatatcggc
    66061 gaaatcctta tcgataaccc gaaagtcctc gaactggcac gtcagcatat cgctgcatta
    66121 ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg gcgagatccc gctgttcagc
    66181 cactaccaga tcgagtcgca gatcgagtcc gccttccagc gtgaagtccg tctgccatcc
    66241 ggtggttcca ttgttatcga cagcaccgaa gcgttaacag ccatcgacat caactccgca
    66301 cgcgcgaccc gcggcggcga tatcgaagaa actgcgttta acaccaacct cgaagccgcc
    66361 gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc
    66421 atcgacatga cgccagtacg ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg
    66481 cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc gctttggcct gctggaaatg
    66541 tcccgccagc gcctgagccc atcactgggt gaatccagcc atcacgtctg cccgcgctgc
    66601 tccggtaccg gtaccgtgcg tgacaacgaa tcgctgtcgc tctctattct gcgtttgatc
    66661 gaagaagaag cgctgaaaga gaacacccag gaagttcacg ccattgttcc tgtgccaatc
    66721 gcttcttatc tgctgaatga aaaacgttct gcggtgaatg cgattgaaac gcgtcaggac
    66781 ggtgttcgct gcgtgattgt gccaaacgat cagatggaaa ccccgcacta ccacgtgttg
    66841 cgcgtgcgca aaggggaaga aacgccgacc ttaagctaca tgctgccgaa gctgcatgaa
    66901 gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc tgaacaacct
    66961 gcactggcaa cctttgccat gccggatgta ccgccagcgc caaccccagc tgaacctgcc
    67021 gcgactgtcg tagccccagc accgaaagct gcaacggcaa caccagcagc tcctgcacaa
    67081 cctgggctgt taagccgctt cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa
    67141 gccaaaccga ccgagcaacc aacaccgaaa gcagaagcga aaccggaacg tcaacaggat
    67201 cgtcgcaaac ctcgtcagag caaccgccgt gaccgtaatg agcgccgcga cacccgtagt
    67261 gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg ccaggcacag
    67321 cagcaaactg ccgagacgcg tgagagccgt cagcaggctg aggtaacgga aaaagcacgt
    67381 accaccgacg agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa
    67441 cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc
    67501 gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca gctcaatcag
    67561 aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt ggttgaagaa
    67621 actgccgctg ccgaaccaat tgttcaggaa gctccagctc cacgcacaga actggtgaaa
    67681 gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc aagaagagaa caatgctgat
    67741 aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgtg
    67801 agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc gccaatgccg
    67861 ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca aagtctggat ccgctatcca
    67921 attgtacgtc cgcaagatgt acaagttgaa gagcagcgcg aacaggaaga agtacaagtg
    67981 cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg aacctgttgt tagcgcgcca
    68041 gttgttgaag aaatggctga agtcgtagaa gcccccgtac cggttgccga accgcaaccg
    68101 gaagtggttg aaacgacgca tcctgaagtg attgccgccg cggtaactga acagccgcag
    68161 gtgattaccg agtctgatgt tgccgtagcc caggaagtcg cagaacacgc agaaccggtg
    68221 gttgaaccgc aggaagagac ggcagatatt gaagaagttg ccgaaactgc tgaggttgtg
    68281 gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga agtcgcagca
    68341 gaagttgaaa cggtggccgc ggttgaacca gagatcaccg ttgagcataa ccacgctacc
    68401 gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg tcacagtgac
    68461 tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca tacggcaacg
    68521 catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt agctcaaagt
    68581 aatcaagccc tggtaactgc cagggctttt ttatttcatg tttgaatcaa catattaaaa
    68641 ttcatcaaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa atgcccgccc
    68701 cggcgatccc agagcgggca gacaaaacat acccagtttc atgatatgtt ttaaagcgag
    68761 cttatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct ttatacgatg
    68821 cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag tccacatcaa
    68881 ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaag gaatccagcg
    68941 actccagttt gttcagctgc gtgcctaatt ccgcgcgaac ggtaaggaca ttattcagag
    69001 agtttttcag tccacggttg gttttatcca atgccgcagc ggcgatttct ttatccgctt
    69061 cgctatccgc gaccggcgtt ttcagggctg cgatggcgct atccagcatg gcaaaaagat
    69121 tggtttcaga agcgctaccg tctggttccg ctaccgcgtt actggtaata ctgtcgaaaa
    69181 ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc atcgacctgt tgcttaatac
    69241 tttctgttcc gccaacataa tcaccgttag cctcactaaa cggcgcagtc tctgttttat
    69301 aaccggcaaa aatgtagcgc ccattaccgt cagtggtgtt cgccagattc agcaactggt
    69361 cacgaagccc ctgaatatcc gtagccagcg aggcccggtc atcgtcactc aaggtgccat
    69421 tgctggcgta gacaattttt tcctgagcat tctggatagc agtggtgacc tggctaagta
    69481 cactctcttc cagtgacact ttttgagtgg cgaaagtacg tgccagcgtg tactggctgt
    69541 tttgcgcctg tgcctgggag agaacaacag cttgtgatgc agcaatggga tcgtctgaag
    69601 ggttaacgac tcgcttaccc gtcgacatct gctcgccgta cttcatccat tctgcctgag
    69661 aattggtgat gccgcgcatg ttttgctggt acatcatctg ggtactgaaa cgcattttat
    69721 ctccccttag cgaatgttaa tcagcgcatc aaaaatcgcg tttgccgtct gcagaacctg
    69781 cgcattcgcc aggtaatact gctgaaaacg ttgcagattt ccgtactcct catcgagatt
    69841 gacaccggaa atcgactgct gctgattgga aagctgcgtc accacattac cttgcgtggt
    69901 gctactggtt ttcaacgtcg cggttttatt accgatatca ctcactaacg aggcataagc
    69961 gtcgttaaag gatttctcac cgcccaccgt tttactgttg ctttgcagat ccagcagggc
    70021 ctgaccgttg cggttgtcgc tatcacccgc atcttcttcg ctcgccatcg cgattttcgc
    70081 ttcgtcggtg attaatacat ccatgttgac gatggcgtca cttactggtt tcagcgtgaa
    70141 gctgtcgtta acggcaggcg ttcctgtaaa cgtcaactcc agaccatcaa atgccacttt
    70201 accgttggca tctggtgtcg ccgtaaaagt ggtattgctg gcaaggcggg tgacctgcca
    70261 ctgattatta tcgaacgaga ttttgtaatc tgtcgccagt accacggagg catcagttac
    70321 cgtggcaccg atcgcaacgt cacctttgtt tttcgtgttt tgcagaaccg cgggcttacc
    70381 gatagtaaag aaatcttcac cagcatcgcc gttagcatca aatccggctt tgtgttggct
    70441 gttgaaagcc tcggcaaatg ccagcgccag ttgtccaagc gtattacgcg tctggtccag
    70501 atcctgagaa cggaatgtca gaatgccgcc cagcgacccg gtattcagta atttctccgg
    70561 gatctcaata ttgcctgccg tcccatcaac gtaagcgaca gtcgtacgag aagggtcggc
    70621 gctggaagga actgccgcca gttgccgcgc cgtacttccc tgaaccagtg agtaaccatt
    70681 ggcatcgtga tgttataagt accgccatcc tgaacgctga cttctacgcc aacaatctgg
    70741 tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga cgcccctgcc
    70801 cccacgcctg tcaggcgcga gatttgatcg ttcaggctgg caatttgttt agcgtaattg
    70861 ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg gtcgcgcaga
    70921 tattgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat cagcgcctgg
    70981 cgcgccgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa gaaatcctgc
    71041 atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt cgacatctgc
    71101 tcatagcggg cggtcaggcc actactttgc gtctgcgcag cacgtaactg gttggtaata
    71161 aacgtatcat actcacgctg cacaccagaa acgtagacgc cattgccaac ccagccgcca
    71221 gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata tccggcaacg
    71281 ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc gttcagtccg
    71341 ctcatggcgt tattgatcaa gctggacatg gaggttcctt ataagccttc aattacagag
    71401 tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca atgttcatgc
    71461 tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctggatcatg ttggtgagtt
    71521 tacgggcata gtgaggatcg gtagcatagc ccgcgtcctg taggacctgc gccccctgtt
    71581 ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt acgtgttaac agcccaacgt
    71641 aatccgacaa tgcttccaga tacgagctgt agacccgaaa cttcgctttt actttcttcg
    71701 cttcgccgtt ttcatattca gtcgtggtga tttcagtgac ttgccctttc cagttgccag
    71761 aggctttgac accaaacagg ttatagcttg gctcgccgtt ttcgcggcgg atttgccgtt
    71821 gcccccaacc agattcaagc gcagcctgag cgaggatcaa atgatgtggc acaccgcttt
    71881 gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgctcta ctgtcacccg
    71941 gcagcgaatc atcgtagtta cgtggtacgg ccttttgcac cagctgcgaa agcgtctggt
    72001 tttgataacg caccacggtt tcgagcggga atttcatcgg tgctgctggc gtggactcct
    72061 ctggcaatgg ttgttctggc gtcatctgtt taaccatcat ctctgcaagc cccagacctt
    72121 tgcccgccgt catctgttgg gcaatttgct ggtcatacat actggtatac agacgagtgt
    72181 gctcgctgct gaacaggcca tcttttggta aagcgtcgcg catgcttttc aacatcatct
    72241 gcacgaacat cccttccacc tgacgggcca ccggacggat atttgccgcc ggatcttcgc
    72301 tcgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt gccagtagtt
    72361 tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg cactttgcat
    72421 tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga gcgcacgcac
    72481 cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc tctggcgtaa
    72541 atcgatctgc gtttgtggag taacaacagt ctgtccacca ccaaacggtg tatctggctg
    72601 gctgacattg gcctgacgat taactgttac tgagagattt ccctgcgcta ccgcgcagct
    72661 gtcgagggtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa tcactacttt
    72721 agcgtcctgc ggggtgacat taacctgcat attctggata tcggcaagga aacggacctg
    72781 ggaactgttg ccacttggta cgcgcacctg aatagtccgc gcatctaacg cggtggcgct
    72841 gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc tgaaatcttc
    72901 gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggca attcacgttc
    72961 aataaccgca ccattggtga tccgtccacc gttcagttgg ttaacctgaa cactgctacc
    73021 gcctgcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg catacacctg
    73081 actgtcaacg cccttaagcg gtgtcatcaa caacgtacct ccacgcaagc ttttggcatt
    73141 acccatggaa gaaaccacca catcgatggt ttgcccctga cgtccaaacg gaggaagtga
    73201 cgctgtcacc attaccgcag cgacgttttt tagctgcata ttggtgcccg tcggaacggt
    73261 aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg tctgggttgt
    73321 ctggtcacca gtgccatcca gccccactac caagccatag ccaatcagtg agttttgcct
    73381 taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg tcgtaaccag
    73441 tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat tggcgacagg
    73501 ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta gccattgcct
    73561 acgtattcaa tacgcgcatc cgccacctga gtagacggta cggtattgct gccgctgata
    73621 gtgcgtggat taaccacgcc cgagaagcga ataaattcgg taccctgatt aatggcaatc
    73681 tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac cgtcaccgtc
    73741 aacgtgccgc taaaggtatt gctggcattg gccccgccct ttccgttgaa cgtgttacca
    73801 ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata gcgcggcaca
    73861 gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga gcttttgctg
    73921 gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg tggtcgacga
    73981 tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa aatagaaccg
    74041 ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg caccagcggc
    74101 gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga aatggcataa
    74161 gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcagtaaac cggtgaacct
    74221 gccaccggtt atgccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac
    74281 cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc
    74341 cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc
    74401 cggcgtgctt tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt tttcgccaat
    74461 gctctccagt ccggtatcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg
    74521 agctgcctgg ccttattggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa
    74581 cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg tcaccagctg
    74641 cccgttctga tccacctgga aagagccgtc acggatatag gctgatgagc catctggcaa
    74701 cattacctgg aaaaagccct gccctttaat cgcgacatct ttgctgttat tggtctgcga
    74761 cagattgccc tggctgtgta agcgttcagt ggcgaccggg cgtacccccg tgccgatttg
    74821 taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg
    74881 ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac tgacgtttgc
    74941 caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat
    75001 ccataatgaa ctgatcatat aatgtcctgt agtggattgc tgacaagcag agcagaagca
    75061 cgcgacagtg tgaatttccc ctcaccctaa ccctctcccc aaaggggcga gggaatagtc
    75121 cgcgcgcttt tattgcaatg tcatcaatcg ttcaattaac tcatcgacag cagttggttg
    75181 gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc aaaacgccgc
    75241 gcgctggcaa tcatgtcgct cattgccgca acggcattga cgttactgcc ttccagaacc
    75301 cccgacatca cacgcaaggt tggatctgcc tgcagtaccg gccctcgcgt ggcctgggtt
    75361 tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc cgtggctttc
    75421 accagtttca gacgccctac tggcgcaact gtatttgccg gatcgcccgg attgagcgcc
    75481 gagattgtgc catcggcagc aatcgtgatt tccgcccctt ccggcacagc aattggcccc
    75541 gcctcgccta tcaccggatg cccctgaatt gtcagttgcc cggtgggatc aacctgaatg
    75601 ctgccattac gcgtataccc ttcgctgccg tcagcggtct gcacggccag ccatcctgct
    75661 gcaacgcaac gtccagcggg cgcgaggtgt aatccatctt gccgggcgtc atgtctgcac
    75721 ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc accggcactg
    75781 cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc agattactgg
    75841 cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcagta taaattgcgt
    75901 gatccattga gctatcccgt cagcgattag cgtaagttaa cccgcgtgtt gaggatctgg
    75961 tcctgggttt tgatggtctg ggcgttagac ttatagttac gctgggcaac gatcatattg
    76021 accagttctt tactgagatc gacgttggac gcttccagcg caccgttggt cagggtgcca
    76081 aagtttcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt cgcagaccag
    76141 acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag tacaatctgc
    76201 cccagcagtt gggtttgttc gttggaattg ttgccgacaa ccgtaccgtc atcattgatt
    76261 tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac aatgttgtta
    76321 gcgccggtat tttgctgcat ggagttgagg aagctcagac taaacgtggc gggttctgca
    76381 ccgttaattg cgccggttgc gatattattc gccatcgcac catccactaa tgtgccatta
    76441 gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact gctatcctgg
    76501 gtgtagacct gccagttatt atccccggtc ttcacaaaat agacgctcat gtcatgagca
    76561 ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg
    76621 gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat ctgcatcgac
    76681 gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt ggtcggattc
    76741 gccccttgct gaatagtcgg cggcgtaccg gttaccgggt aacccgtcag ctgtaaacct
    76801 tgcgtattca acaggttacg gttttcatcc agcttaaatt gtccgttacg gctgtagaac
    76861 accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga
    76921 cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt
    76981 acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta
    77041 aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca
    77101 gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct
    77161 taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta
    77221 ttaccgctgt tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc
    77281 agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg
    77341 ctgccatcag tcaacgaacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca
    77401 atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct
    77461 gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg
    77521 ccggttcccg caagaacagt tgtgccgggg atcatcacgc cgtgaccgat caggttactg
    77581 gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt
    77641 ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc
    77701 attggattgg tcgggtcctg gtttttcagc tgcgccacca gcaaagtcag aaaactgctt
    77761 tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta
    77821 tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg
    77881 taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc
    77941 tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc ggcattttta
    78001 cgtagccctt tgcatctgcc agcggattac ctggttcata aaccagtttg tccggggcct
    78061 gactttctta acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac
    78121 ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca cgctatcagc
    78181 atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc
    78241 ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat
    78301 catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca ggctgttatc
    78361 ggcaaactgg gtgcgttcgc gatccatatc gacggtatta ccgtcaaacg aaggctggtc
    78421 cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc gcctgcgccg ggatgtgttg
    78481 cgttgaggtc atcgtcagtg caaccacact ggttgcatcc cgtccacgtt gcatgacttt
    78541 tttaagttca ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat cggcattggc
    78601 gatgtttgct gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct cttgttgaaa
    78661 acgtaaggcg gcgtcgagct tatcgagcat atctcctccg caggtatcaa aattctgcca
    78721 tcagcttaaa tgcctttacg cgcgcgttat cggcggaata aacgcaaaat gggtcgctat
    78781 ttatgccgtt gatggtcatt gcggacaggt acaattcacg ttgtagaaat ggctgggggc
    78841 gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg atactgttca gtccgttaag
    78901 tacggcgagc aatctcacat cgcaattgca caactttttt agcgcccaac tcgcgggggt
    78961 aagtgatgag gttcgtgttt ctattcgtac agcgcccaat ctactaccgc catgcgagca
    79021 gccattgctt tcgatgagca ataattcccg cctgtggggc aatgtgaatg tgttggcacg
    79081 ctgcggtaac gacaaacgat atttacaggt taatgtgcag gccacaggaa attatgtggt
    79141 tgccgcgatg cctattgtgc ggggaggaaa gctggaagct ggcaatgtca aactgaaacg
    79201 tggacgcctg gataccctgc caccgcgtac ggtgctggat atcaatcaac ttgttgatgc
    79261 cgttagcctg cgcgatctat cacccgatca acctatccag ttaacccagt ttcgccaggc
    79321 atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc agcggtgatg ggtttagcgc
    79381 caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca cagaatgcgc gggtgcgcat
    79441 ggtatcggga caggtagtca gcggcgttgt tgatgcagat gggaatattc ttataaacct
    79501 gtaagctgtt aaagattacc cgtcccttgc cgataaataa gcaacacatg ataaaagcgc
    79561 cctcaatgag gaataaacca tgagtattga tcgcacttcg cctctgaagc ctgtaagcac
    79621 cgttcaaccg cgcgaaacca ccgacgcgcc ggtaacgaac agccgggcgg caaaaacaac
    79681 cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg
    79741 cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc gtaacggtga
    79801 actaaaaatg gacaccggca aaattgccga tgcgctgatc aacgaagcgc agcaagactt
    79861 gcagagtaac tgatcgtatg acacgtcttg cagagatcct cgaccagatg tccgctgtgc
    79921 ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg gggcagatca
    79981 acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg acgctggatt
    80041 acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat attagtcaac
    80101 gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa cataacggct
    80161 ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc
    80221 aggaaccgac gctatatggg gcgaacggtc agacctcaac accccatcgc ggcagtaaaa
    80281 agatttcgat ctgaatatta tctccggcct gcactgcagg ccggaatgca ttgttacacc
    80341 gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc gaagtacgcg
    80401 gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat ggtacccaat
    80461 gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac cagtaccgcc
    80521 accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat tttctgctta
    80581 cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat agaaagtgac
    80641 agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt taaaatcagc
    80701 gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa gccaggagcc
    80761 aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg
    80821 gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac ggttaacgga
    80881 cctgaaagaa tgcccaacgc aaccgcactc ggcagtgcca acaggaaaca aagacgcaac
    80941 ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact tttcgacagc
    81001 gacggcagca aaatggtgcc aagcgccacg cccagcacac cggagggaaa ctccattaag
    81061 cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg
    81121 ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc catctgtttc
    81181 accacgcgca ttgctccagc atcgtggaag ttaatgcgcg gcaggaccag catgccgatc
    81241 ttcttcaggt gcggtagctg atacaccagc tgcaggatgc cgccgaccgt aacagcccac
    81301 gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg
    81361 ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga
    81421 atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt aatctttagt
    81481 agctggctgg tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg
    81541 atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc aagtgtcagc
    81601 aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt acttttatat
    81661 tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat acggcgtaac
    81721 aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt
    81781 ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt catcgagctg
    81841 acggcggcca gcgattttaa taaattcatc ggtgttctaa tccggaccca cgggcaaaac
    81901 gcctgcaagg caggcgctga aagaggcgaa tagtctacct agattatggt gaattgctac
    81961 cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt caacgatacg
    82021 ttgcgccagc actgcctatt cgccggcggt ttgcggaact gtctgatttt gcacgcattc
    82081 aatgaagtgc cgcgcacagc cgacaaatcc acgttgctca agagtgctct gccaaccagg
    82141 aatcggttta ttcactacac cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat
    82201 gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc cggcacggcg
    82261 atgcatacaa gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat
    82321 ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc cgcccgacaa
    82381 ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat aaagatcgtg
    82441 tggcccgacg ctattgctac gatgtttatc cattcttagc gaggctgcgg tggcgagttg
    82501 cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag
    82561 ttttttccgc gccgccagtt ccaccagcct ttcagcatcg cgcagatttt ctgccagcgg
    82621 tttatcgaca cagacatgta cccccgcatt gagtaacgta ctgaccacgt caaagtggct
    82681 ggcggtgctg gagtgcacaa aaaccgcatc acaactggcg gcaaggctgg ataacgaatc
    82741 ggcataagga atgcgccagc tgtcacaaat tggcagggct ttggcgcgcg taggcgacca
    82801 ggctccttgt aacgtccagt cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc
    82861 aatgccgcct aatcccacta cgccgatacg taattttttc actttaatct cccagatggg
    82921 ccagcaacga ataaagacgt tgtttcagtt ctgccacttc gatttccagg gcttcgacgc
    82981 gggtctgtaa atcaccgtca accatgttcg acatagccgt taccgccggc tgatcttcaa
    83041 cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg cgggccagac
    83101 gcaccacaaa aggaccatct tcgcgattcg ccagttgttc cagcgtcgac tccacttccg
    83161 ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg
    83221 caccacgcaa taacaacgtg gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc
    83281 caaattctga attacaaaaa cgttgctcat atttggtgac ccgattacca aaaccgctca
    83341 ctgtgcgtag ataatgacgt ttgaccagat tatccagctg ttcctgcact tcggattcac
    83401 tcagattcat gaccggttca cggttcgttt tctgattaca ggccgtgact acaccattga
    83461 ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa ccaattacgc
    83521 gagcttccag ggcagtaagt tgatatttca tgagattctc cttagcggcc gggcgtccag
    83581 tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat caacagatag
    83641 tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta
    83701 ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg catataacga
    83761 atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg cccaatcgaa
    83821 taaccgagat agcaggcatg aaaagagccg cgaacaacat tggaaaaatt ggcaacgcca
    83881 ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc ttgtttatga
    83941 aattcgttaa tcatccctag cctggcctgc cagcctgatg gataacagtg gctttcgtcg
    84001 cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc cgccagacgc
    84061 caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa gcgcactttt
    84121 ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac gtctttaata
    84181 cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt
    84241 tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa
    84301 tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa agtgctcatc
    84361 ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat
    84421 ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt cccgctgatc
    84481 tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat tgctctcggt
    84541 ctacgccagt ttattcagca aggtctgggt attttcggcg gcgcaattgc cgaccgcttt
    84601 ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt cgccacaatg
    84661 ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg actcggcggc
    84721 acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc acaacaacgt
    84781 ggtcgttttt tctcgctgtt gatgatgcag gacagtgcca gtgcggtcat tggcgcattg
    84841 ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg ggccgttttg
    84901 tttgtgctat gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact ctccaccgta
    84961 cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt tgtcacctat
    85021 gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgat gctgccaatt
    85081 atggtcaacg acgtggctgg cgcgccctct gccgttaaat ggatgtatgc cattgaagcg
    85141 tgtctgtcgt taacgttgct ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg
    85201 gaacaccggt tgatggctgg gctgttgata atgtcattaa gcatgatgcc ggtgggcatg
    85261 gtcagcggcc tacaacaact tttcaccctg atttgtctgt tttatatcgg gtcgatcatt
    85321 gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc
    85381 tatatggggt ttagccgtct gggtctggcg attggcggcg ctattggtta tatcggtggt
    85441 ggctggctgt ttgacctggg caaatcggcg caccagccag agcttccgtg gatgatgctg
    85501 ggaattattg gcatcttcac tttccttgcg ctgggttggc agtttagtca gaaacgcgcc
    85561 gcgcgtcgtt tgctggaacg cgacgcctga tttgatactc ccccctgact cctccatact
    85621 gaaagtgaac tgacagccaa atggaggagt cacgtgaagc tatacattta cgatcactgc
    85681 ccttactgcc tcaaagcccg catgattttc ggcctgaaaa atatccccgt cgaattacat
    85741 gttctgctca acgacgacgc agaaacaccc acccggatgg tcggtcaaaa acaggttccc
    85801 attctgcaaa aagatgacag ccgctatatg ccagaaagca tggatatcgt tcactatgtc
    85861 gataaactcg acggcaaacc gttactgacc ggcaaacgtt cccctgccat tgaagagtgg
    85921 ctgcgcaagg tcaatggcta cgccaacaaa ctgctgttgc cgcgttttgc caaatcggca
    85981 tttgatgagt tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc
    86041 gcgggtaatt ttgccgacct gctggcccac tctgacggtc tgattaagaa tatcagcgat
    86101 gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg
    86161 gaagatgata ttcagctatt tccgctactg cgtaatctga cgctggtagc cggaatcaac
    86221 tggccaagcc gcgttgctaa ttaccgcgat aatatggcga aacagacaca aatcaatttg
    86281 ttatcatcaa tggcgattta atcctgaccc gccgcggcag cctgtattcg cgggttgccg
    86341 ccgttttcag ttttttctgc tggcgagttt atcgctggtg acacatgctg cgttgtgtca
    86401 ggatattgac gcagatttca tgtcgaggac taccatgaac aagtttttat ttgccgctgc
    86461 attgatcgtc agtggcctgc tcgttggctg taatcaactc acccaataca ccatcaccga
    86521 acaagaaatt aaccagtcgc ttgcgaaaca taataatttc tcaaaagata tcggtttacc
    86581 cggcgtggct gacgcccata ttgttctgac aaacttgacc agccaaattg gtcgcgaaga
    86641 gccgaataag gttaccctaa ccggagacgc caatctggac atgaactccc tgttcggtag
    86701 tcagaaagcg accatgaaac tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg
    86761 tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca
    86821 aacggtgatg caaacgttgc ttccctattt gaaccaggca ttacgcaatt actttaacca
    86881 gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact
    86941 ggcgaaaggc attgaagtga agccaggcga aattgtcatt ccgtttacgg attaatgacg
    87001 agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct tatcctttgt
    87061 gtccggcaaa aacatccctt cagccggagc atagagatta atgactgcac catcccaggt
    87121 attaaagatc cgccgcccag acgactggca ccttcacctc cgcgatggcg acatgttaaa
    87181 aactgtcgtg ccgtatacca gcgaaattta tggacgggct atcgtaatgc ccaatctggc
    87241 tccgcccgtg accacagttg aggctgccgt ggcgtatcgc caacgtattc ttgacgccgt
    87301 acctgccggg cacgatttca ccccattgat gacctgttat ttaacagatt cgctggatcc
    87361 taatgagctg gagcgcggat ttaatgaagc cgtgttcacc gctgcaaaac tttacccggc
    87421 aaacgcaacc actaactcca gccacggcgt gacgtcaatt gacgcaatca tgccggtact
    87481 tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat ggtgaagtga cacatgcaga
    87541 tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca
    87601 gcgcctgact gcgctgaaag tcgtttttga gcacatcacc accaaagatg ctgccgacta
    87661 tgtccgtgac ggaaatgaac ggctggctgc caccatcact ccgcagcatc tgatgtttaa
    87721 ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa
    87781 acgcaatatt caccaacagg cattgcgtga actggtcgcc agcggtttta atcgagtatt
    87841 cctcggtacg gattctgcgc cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc
    87901 gggctgcttc aacgccccaa ccgcgctggg cagttacgct accgtctttg aagagatgaa
    87961 tgctctgcag cactttgaag cattctgttc tgtaaacggc ccgcagttct atggcttgcc
    88021 ggtcaacgac acattcatcg aactggtacg tgaagagcaa caggttgctg aaagcatcgc
    88081 actgactgat gacaccctgg tgccattcct cgccggggaa acggtacgct ggtccgttaa
    88141 acaataaaaa tcgtagcttc ctgttgtcat taggttattt tatctgtata aataaccagt
    88201 atattcaaca gggggctatt atgcgaattg aagtcaccat agcgaaaact tctccattgc
    88261 cagctggggc tattgacgcc ctggctggcg aactttcccg ccgtattcag tatgcgtttc
    88321 ctgataatga aggccacgta tcggtacgtt atgccgcagc gaataattta tcggttattg
    88381 gcgcaacaaa agaagataaa cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg
    88441 ccgatgactg gtttgtcagc gaataatatg cagtgatttt tgtttttgcc ggatcgcccc
    88501 ggctttttta ttttacttac gaattttgag tacaaaattt ctacacttcc tcaaaataat
    88561 tgcacaatat gctaaaaaaa tatgaatgac atgctgtttt attgttcata taaatcttaa
    88621 aagcagaaat ttatctgttc ccccctttaa aaattcggtg aataccctta cttattggta
    88681 tactgaagtt gcttcagata aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg
    88741 aattaggggg ccatgatgga aaaaaataat gaagtcattc agactcatcc gctcgtaggg
    88801 tgggacatca gcaccgttga tagctatgat gcgctgatgt tgcgtttgca ctaccagacc
    88861 ccaaataagt ccgagcagga agggactgaa gttggtcaga cgctctggtt aaccactgat
    88921 gttgccagac aatttatttc gatattagaa gcaggaatcg ccaaaattga atccggtgat
    88981 ttcccggtaa acgagtatcg gcgtcattaa catgacatga ccatccaccc aaaaggcacc
    89041 tcaccggtgc ctttaccatt tctgggttgt atatctgcat tcagttaatt actctgctta
    89101 aaccatgaga gcagagagaa aaaaatgaaa tacgatctca tcattattgg cagcggttcc
    89161 gtaggcgctg ccgccgggta ttatgcaacc cgcgccggtt taaacgtgct aatgaccgac
    89221 gcccatatgc caccgcatca acacggcagc caccacggcg atacgcgatt aattcgccat
    89281 gcttatggtg aaggcgaaaa gtatgtcccg ttggtcctcc gcgcgcaaac gctgtgggat
    89341 gaactttccc gccacaacga agatgatccc atttttgtac gctctggtgt cattaacctc
    89401 ggcccggctg actccgcatt tctcgccaac gtcgcccaca gcgccgaaca gtggcaactc
    89461 aacgttgaaa agctcgatgc gcaagggatt atggcccgct ggccagaaat acgcgtcccg
    89521 gacaactaca tcggcttatt tgagactgat tccggttttt tgcgcagcga actggcgatt
    89581 aaaacctgga tccaactggc gaaggaagcg ggctgtgcgc aactgttcaa ctgcccggtc
    89641 accgcaattc gtcatgacga tgatggcgta actattgaaa cggttgacgg tgagtatcag
    89701 gcgaaaaaag cgattgtctg cgcgggaaca tgggtaaaag acctgctccc ggagctgcct
    89761 gtccagcccg tacgtaaagt atttgcctgg tatcaggccg atggccgcta tagcgtgaag
    89821 aataaattcc cggcgtttac cggtgaactg cccaatggcg atcaatatta tggttttccg
    89881 gcagaaaacg acgcgttgaa gattggcaaa cataacggag gccaggttat ccattcagcg
    89941 gatgaacgtg ttccgtttgc ggaagtggcc agtgatgggt cagaagcctt cccgttcttg
    90001 cgcaatgtat tgccgggtat cggttgctgc ctgtacggcg ctgcctgcac ctatgataat
    90061 tcgcctgacg aagattttat tatcgatacc ctacccggcc acgataatac actgctcatt
    90121 accggcctga gtgggcacgg ttttaaattt gcgtcagttt taggggaaat agctgccgat
    90181 tttgcgcaag accaaaaaag cgattttgat ttgacgccat tcaggctttc ccgcttccaa
    90241 taatcatcat tacggcctcc agaatgaggc cgtttttaca ggagtattta atgcgtcggc
    90301 tgttgcacta tctcatcaat aatattcgcg agcatctgat gctttatctt tttctctggg
    90361 gattgttggc aattatggat ctgatctatg tgttttattt ttagttttcc gcactgacat
    90421 aaactgacaa atatttaatc tccctttcat ttagcgtgaa aaattgaatg agattattcc
    90481 tgcttctaca gcactcacca gacatctctg atgctgaacc cactaaactt atgaagattc
    90541 tttcattatt aattcaaaat tattgaaata aaaataaata tcattcaatt tgttattttt
    90601 gtgttgcaat tcattctccg acacgccatt ttctttctct cgcaaattcg tattggatgc
    90661 acttatgtca ttcacaaata cccctgaacg ctatggtgtt atatctgcgg ccttccattg
    90721 gctaagcgca attattgtct acggcatgtt tgctttaggt ctgtggatgg tcacgctcag
    90781 ttattacgat ggctggtatc acaaagcacc cgaactgcat aaaagcatcg gtattttgtt
    90841 aatgatgggg ctggttattc gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct
    90901 gccgagttat tcgcccatga ctcgtcttgc cgcaaaagct ggacatctcg cattgtacct
    90961 attgctgttc gccatcggca tcagcggcta tctgatctca actgccgatg gtaaaccgat
    91021 cagcgttttt ggctggtttg acgtccccgc gacccttgcc gacgctggcg cacaggcaga
    91081 ctttgccggt gccctgcatt tttggctcgc gtggagcgtc gtagtactgt ctgttatgca
    91141 cggatttatg gccctgaagc atcatttcat cgataaagac gacactctga agcgcatgct
    91201 gggaaagtcg tcatctgact atggagtata aaaaatgaaa aaaagcctgc ttggtttaac
    91261 cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc gccgattaca aaattgacaa
    91321 agaaggtcag cacgcctttg ttaacttccg catacagcat cttggctata gctggttata
    91381 tggcaccttt aaagatttcg acggtacttt tacctttgat gaaaaaaatc cggctgccga
    91441 taaagtgaat gtgacaatta acaccaccag cgtcgatact aaccacgccg aacgcgataa
    91501 acatcttcgc agtgcggatt ttctcaatac cacaaaatat ccacaggcaa cattcacctc
    91561 caccagcgtg aagaaagacg gtgacgaact ggatattacc ggcgatctga cgctgaatgg
    91621 cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt cagggcgacg acccatgggg
    91681 cggtaaacgt gcaggctttg aggccgaagg caaaattaag ctcaaagact tcaatatcaa
    91741 gacagattta ggtccagctt ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca
    91801 gcagaagtaa gagtgaaatg ttgccggatg gcacgcatcc ggcaatgata tttattctgt
    91861 tggatcagga atacccagtg ttgtattcag acgtccacga gacttattaa agatcttatt
    91921 gccattttca cgtcctgccc gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc
    91981 gcagcaaatc tcactacaac aacctttgta tttttccgcg catactgggc actgaataaa
    92041 cagcaggtgg cagccatcat ttttacagtt ggtatggctg tcgcacggcg cgccgcactg
    92101 gtggcaatgc gcgataatct catccgatat acgttcgccc atccgctcgt caaaaacaaa
    92161 atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat
    92221 aattccgccc tcgatatgcc agactttgtt gaatccgtta tgtttcatcc aggcactggc
    92281 cttttcacaa cgaatgccgc cggtgcagta catgacgatt tttttatctt tatgtgcctg
    92341 catcatctcg actgcttttg gcagctgctc acggaaggta tctgccggaa tttcgagcgc
    92401 gttttcaaag tgccccactt catactcata gtggttacgc atgtcgataa atagtgcatc
    92461 gggatcgtca agcatggcgt tcacttccgc cgcttgcaga tactcaccaa cattgctggc
    92521 atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga tcgcgtacct tcatgcgcag
    92581 tacccagaag gatttcccgt catcatccaa cgcgatattc aggcgtaaac cctctaaagc
    92641 cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat
    92701 ttgcgcgtta atgccctcat gcgccagata cactcgccca aaaacattca gcgcggtaaa
    92761 cagctgatat aaagcgtcac gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa
    92821 cgaaatggtg gttcgcggtt cgctctcagc caacattttg gcttttaacg cgtcgttgga
    92881 aatgcggttg tgtaacactg gcatggtgta cggttcctgc gagatgggaa agtaaaaatc
    92941 cgcggcatga tatagcaatt atcgataatt aacatccaca cattttacgc tacatttgcg
    93001 cattaaaaat tatttgttat ttacaagcgc ggcaatttcg cccagtcttc agccacaaat
    93061 tttggttgcg ggcgaaaaaa tgcgacaata catacaattg cccgtatagg ttgaaaaaca
    93121 ggattgatat gacgaatcta cccaagttct ccaccgcact gcttcatccg cgttattggt
    93181 taacctggtt gggtattggc gtactttggt tggtcgtgca attgccctac ccgattatct
    93241 accgcctcgg ttgtggatta ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa
    93301 ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa
    93361 tggtggtgaa gaatttcgaa tccgttggca tgggcctgat ggaaaccggt atggcgtggt
    93421 tctggccgga ccgccgaatc gcccgctgga cggaagtgat cggcatggaa cacattcgtg
    93481 acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat ccattttctg acactggagc
    93541 tgggcgcgcg gcagtttggt atgcaggaac cggggattgg cgtttaccgc ccgaacgata
    93601 atccactgat tgactggcta caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg
    93661 accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg
    93721 caccagatca tgattacggc ccgcgctcaa gcgttttcgt cccgttattt gccgttgagc
    93781 aggctgcgac cacgaccgga acctggatgc tggcacggat gtccggcgca tgtctggtgc
    93841 ccttcgttcc acgccgtaag ccagatggca aagggtatca attgattatg ctgccaccag
    93901 agtgttctcc gccactggat gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg
    93961 aaaaatgcat catgatggca ccagagcagt atatgtggtt acaccgtcgc tttaaaacac
    94021 gcccggaagg cgttccttca cgctattaaa tctcccatgc cggatgcttc agaatggcat
    94081 ccggcattac cacagcaaat ccccctgatt tagcgataaa agctctctgg attgcgcccc
    94141 ctggaagtcg ggcgcataat tagtgtgttt atcttttctt cttatgttca ccgcgcctgg
    94201 cgcaccaaca gcggattgct atgtcaccct gtgaaaatga cacccctata aactggaaac
    94261 gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg ggccgccttc agtctggtaa
    94321 tgcccttctt acccctctac gttgagcagc ttggtgttac cggccactcc gccctgaata
    94381 tgtggtccgg tattgtcttc agtataatgg gttcctaaac acatcatttt aggtattgaa
    94441 tatgagacgg gcaatcagct acatcagatt ttcaagtgaa cgacaactga aaggggactc
    94501 tgtacgccga caatctaagc tggttactga ctggcttgat aagaaccctg agttttactt
    94561 ggattcatcc ttgagcttta aagatttggg gaaaagtgct tttagtggta agcacctgaa
    94621 aggtggtcta ggtgactttt taaccgctat cgagaaaggg cttgttaaag ctggtgatac
    94681 gctactaatc gagagtttag accgtctgtc acgtcaggat attgatattg cttctgagct
    94741 attacgccgt atcttaaggg ctggtgtaga tgtagtcaca ctgagcgatg gagaacatta
    94801 cacacgagaa agt
//
LOCUS       DAJVPP010000002        92328 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVPP010000002 DAJVPP010000000
VERSION     DAJVPP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 92328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.002"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            239..580
                     /locus_tag="OQM34_000108"
     CDS             239..580
                     /locus_tag="OQM34_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1450699.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(571..1497)
                     /locus_tag="OQM34_000109"
     CDS             complement(571..1497)
                     /locus_tag="OQM34_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1450700.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1587..2585
                     /locus_tag="OQM34_000110"
     CDS             1587..2585
                     /locus_tag="OQM34_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1450701.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2582..2800)
                     /locus_tag="OQM34_000111"
     CDS             complement(2582..2800)
                     /locus_tag="OQM34_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1450702.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2802..4817)
                     /gene="ligA"
                     /locus_tag="OQM34_000112"
     CDS             complement(2802..4817)
                     /gene="ligA"
                     /locus_tag="OQM34_000112"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1450703.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFVGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4888..5874)
                     /gene="zipA"
                     /locus_tag="OQM34_000113"
     CDS             complement(4888..5874)
                     /gene="zipA"
                     /locus_tag="OQM34_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1450704.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6104..6865
                     /gene="cysZ"
                     /locus_tag="OQM34_000114"
     CDS             6104..6865
                     /gene="cysZ"
                     /locus_tag="OQM34_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1450705.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPAFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7050..8021
                     /gene="cysK"
                     /locus_tag="OQM34_000115"
     CDS             7050..8021
                     /gene="cysK"
                     /locus_tag="OQM34_000115"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1450706.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8405..8662
                     /gene="ptsH"
                     /locus_tag="OQM34_000116"
     CDS             8405..8662
                     /gene="ptsH"
                     /locus_tag="OQM34_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1450707.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8707..10434
                     /gene="ptsI"
                     /locus_tag="OQM34_000117"
     CDS             8707..10434
                     /gene="ptsI"
                     /locus_tag="OQM34_000117"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1450708.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10475..10984
                     /gene="crr"
                     /locus_tag="OQM34_000118"
     CDS             10475..10984
                     /gene="crr"
                     /locus_tag="OQM34_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1450709.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11026..11877)
                     /gene="pdxK"
                     /locus_tag="OQM34_000119"
                     /gene_synonym="thiJ"
     CDS             complement(11026..11877)
                     /gene="pdxK"
                     /locus_tag="OQM34_000119"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1450710.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11982..12356
                     /locus_tag="OQM34_000120"
     CDS             11982..12356
                     /locus_tag="OQM34_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1450711.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12389..13112
                     /locus_tag="OQM34_000121"
                     /pseudo
     CDS             12389..13112
                     /locus_tag="OQM34_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13316..14227)
                     /gene="cysM"
                     /locus_tag="OQM34_000122"
     CDS             complement(13316..14227)
                     /gene="cysM"
                     /locus_tag="OQM34_000122"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS1450712.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSLGAGI"
     gene            complement(14361..15458)
                     /gene="cysA"
                     /locus_tag="OQM34_000123"
     CDS             complement(14361..15458)
                     /gene="cysA"
                     /locus_tag="OQM34_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1450713.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15448..16323)
                     /gene="cysW"
                     /locus_tag="OQM34_000124"
     CDS             complement(15448..16323)
                     /gene="cysW"
                     /locus_tag="OQM34_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1450714.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16323..17156)
                     /gene="cysT"
                     /locus_tag="OQM34_000125"
     CDS             complement(16323..17156)
                     /gene="cysT"
                     /locus_tag="OQM34_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1450715.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVTLSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17156..18172)
                     /gene="cysP"
                     /locus_tag="OQM34_000126"
     CDS             complement(17156..18172)
                     /gene="cysP"
                     /locus_tag="OQM34_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1450716.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18476..19267)
                     /gene="ucpA"
                     /locus_tag="OQM34_000127"
     CDS             complement(18476..19267)
                     /gene="ucpA"
                     /locus_tag="OQM34_000127"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1450717.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19454..20254)
                     /gene="murR"
                     /locus_tag="OQM34_000128"
     CDS             complement(19454..20254)
                     /gene="murR"
                     /locus_tag="OQM34_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1450718.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20418..21314
                     /gene="murQ"
                     /locus_tag="OQM34_000129"
     CDS             20418..21314
                     /gene="murQ"
                     /locus_tag="OQM34_000129"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1450719.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21318..22742
                     /gene="murP"
                     /locus_tag="OQM34_000130"
                     /pseudo
     CDS             21318..22742
                     /gene="murP"
                     /locus_tag="OQM34_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(22922..23821)
                     /gene="yfeX"
                     /locus_tag="OQM34_000131"
     CDS             complement(22922..23821)
                     /gene="yfeX"
                     /locus_tag="OQM34_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1450720.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(23917..24492)
                     /locus_tag="OQM34_000132"
     CDS             complement(23917..24492)
                     /locus_tag="OQM34_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1450721.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24553..25002)
                     /locus_tag="OQM34_000133"
     CDS             complement(24553..25002)
                     /locus_tag="OQM34_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1450722.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(24989..25414)
                     /locus_tag="OQM34_000134"
     CDS             complement(24989..25414)
                     /locus_tag="OQM34_000134"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1450723.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25628..26497
                     /gene="amiA"
                     /locus_tag="OQM34_000135"
     CDS             25628..26497
                     /gene="amiA"
                     /locus_tag="OQM34_000135"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1450724.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26501..27400
                     /gene="hemF"
                     /locus_tag="OQM34_000136"
     CDS             26501..27400
                     /gene="hemF"
                     /locus_tag="OQM34_000136"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1450725.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27406..28458)
                     /gene="eutR"
                     /locus_tag="OQM34_000137"
                     /pseudo
     CDS             complement(27406..28458)
                     /gene="eutR"
                     /locus_tag="OQM34_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28504..29004)
                     /gene="eutK"
                     /locus_tag="OQM34_000138"
     CDS             complement(28504..29004)
                     /gene="eutK"
                     /locus_tag="OQM34_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1450726.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29017..29676)
                     /gene="eutL"
                     /locus_tag="OQM34_000139"
     CDS             complement(29017..29676)
                     /gene="eutL"
                     /locus_tag="OQM34_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1450727.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            30858..31649
                     /locus_tag="OQM34_000140"
     CDS             30858..31649
                     /locus_tag="OQM34_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450728.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32368..32763
                     /locus_tag="OQM34_000141"
     CDS             32368..32763
                     /locus_tag="OQM34_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1450729.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32781..33044
                     /locus_tag="OQM34_000142"
     CDS             32781..33044
                     /locus_tag="OQM34_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450730.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            33067..33609
                     /locus_tag="OQM34_000143"
     CDS             33067..33609
                     /locus_tag="OQM34_000143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450731.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33748..34035
                     /locus_tag="OQM34_000144"
     CDS             33748..34035
                     /locus_tag="OQM34_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450732.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            34074..34280
                     /locus_tag="OQM34_000145"
     CDS             34074..34280
                     /locus_tag="OQM34_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450733.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34592..35077
                     /locus_tag="OQM34_000146"
     CDS             34592..35077
                     /locus_tag="OQM34_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1450734.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            35058..35336
                     /locus_tag="OQM34_000147"
     CDS             35058..35336
                     /locus_tag="OQM34_000147"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450735.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            35329..36789
                     /locus_tag="OQM34_000148"
     CDS             35329..36789
                     /locus_tag="OQM34_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1450736.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36823..37632
                     /gene="murI"
                     /locus_tag="OQM34_000149"
     CDS             36823..37632
                     /gene="murI"
                     /locus_tag="OQM34_000149"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1450737.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37751..38445)
                     /locus_tag="OQM34_000150"
     CDS             complement(join(37751..38199,38199..38445))
                     /locus_tag="OQM34_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1450738.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(38964..40337)
                     /gene="eutA"
                     /locus_tag="OQM34_000151"
     CDS             complement(38964..40337)
                     /gene="eutA"
                     /locus_tag="OQM34_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCS1450739.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40312..40791)
                     /gene="eutP"
                     /locus_tag="OQM34_000152"
     CDS             complement(40312..40791)
                     /gene="eutP"
                     /locus_tag="OQM34_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS1450740.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40804..41139)
                     /gene="eutS"
                     /locus_tag="OQM34_000153"
     CDS             complement(40804..41139)
                     /gene="eutS"
                     /locus_tag="OQM34_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCS1450741.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(41432..43711)
                     /gene="maeB"
                     /locus_tag="OQM34_000154"
     CDS             complement(41432..43711)
                     /gene="maeB"
                     /locus_tag="OQM34_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1450742.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            44000..44936
                     /gene="tal"
                     /locus_tag="OQM34_000155"
                     /pseudo
     CDS             44000..44936
                     /gene="tal"
                     /locus_tag="OQM34_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
     gene            44956..46959
                     /gene="tkt"
                     /locus_tag="OQM34_000156"
     CDS             44956..46959
                     /gene="tkt"
                     /locus_tag="OQM34_000156"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1450743.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(47062..48105)
                     /locus_tag="OQM34_000157"
     CDS             complement(47062..48105)
                     /locus_tag="OQM34_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1450744.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(48231..48806)
                     /gene="nudK"
                     /locus_tag="OQM34_000158"
     CDS             complement(48231..48806)
                     /gene="nudK"
                     /locus_tag="OQM34_000158"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1450745.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(48874..50853)
                     /gene="aegA"
                     /locus_tag="OQM34_000159"
     CDS             complement(48874..50853)
                     /gene="aegA"
                     /locus_tag="OQM34_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS1450746.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            51059..52776
                     /gene="narQ"
                     /locus_tag="OQM34_000160"
                     /pseudo
     CDS             51059..52776
                     /gene="narQ"
                     /locus_tag="OQM34_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            52978..56091
                     /gene="acrD"
                     /locus_tag="OQM34_000161"
     CDS             52978..56091
                     /gene="acrD"
                     /locus_tag="OQM34_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCS1450747.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(56190..56249)
                     /gene="ypfM"
                     /locus_tag="OQM34_000162"
     CDS             complement(56190..56249)
                     /gene="ypfM"
                     /locus_tag="OQM34_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1450748.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            56630..56986
                     /locus_tag="OQM34_000163"
     CDS             56630..56986
                     /locus_tag="OQM34_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1450749.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            56990..58117
                     /gene="dapE"
                     /locus_tag="OQM34_000164"
     CDS             56990..58117
                     /gene="dapE"
                     /locus_tag="OQM34_000164"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1450750.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            58145..58345
                     /locus_tag="OQM34_000165"
     CDS             58145..58345
                     /locus_tag="OQM34_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1450751.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(58455..59153)
                     /gene="ypfH"
                     /locus_tag="OQM34_000166"
     CDS             complement(58455..59153)
                     /gene="ypfH"
                     /locus_tag="OQM34_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1450752.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(59227..61242)
                     /gene="tmcA"
                     /locus_tag="OQM34_000167"
     CDS             complement(59227..61242)
                     /gene="tmcA"
                     /locus_tag="OQM34_000167"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1450753.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(61257..62120)
                     /locus_tag="OQM34_000168"
     CDS             complement(61257..62120)
                     /locus_tag="OQM34_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1450754.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(62288..63001)
                     /gene="purC"
                     /locus_tag="OQM34_000169"
     CDS             complement(62288..63001)
                     /gene="purC"
                     /locus_tag="OQM34_000169"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1450755.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(63214..64248)
                     /gene="bamC"
                     /locus_tag="OQM34_000170"
     CDS             complement(63214..64248)
                     /gene="bamC"
                     /locus_tag="OQM34_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1450756.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(64265..65143)
                     /gene="dapA"
                     /locus_tag="OQM34_000171"
     CDS             complement(64265..65143)
                     /gene="dapA"
                     /locus_tag="OQM34_000171"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1450757.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            65289..65861
                     /locus_tag="OQM34_000172"
     CDS             65289..65861
                     /locus_tag="OQM34_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1450758.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            65861..66331
                     /gene="bcp"
                     /locus_tag="OQM34_000173"
     CDS             65861..66331
                     /gene="bcp"
                     /locus_tag="OQM34_000173"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1450759.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            66584..67201
                     /gene="hyfA"
                     /locus_tag="OQM34_000174"
     CDS             66584..67201
                     /gene="hyfA"
                     /locus_tag="OQM34_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1450760.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            67201..69218
                     /gene="hyfB"
                     /locus_tag="OQM34_000175"
                     /pseudo
     CDS             67201..69218
                     /gene="hyfB"
                     /locus_tag="OQM34_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            69229..70176
                     /gene="hyfC"
                     /locus_tag="OQM34_000176"
     CDS             69229..70176
                     /gene="hyfC"
                     /locus_tag="OQM34_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1450761.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            70193..71632
                     /gene="hyfD"
                     /locus_tag="OQM34_000177"
     CDS             70193..71632
                     /gene="hyfD"
                     /locus_tag="OQM34_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCS1450762.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            71644..72294
                     /gene="hyfE"
                     /locus_tag="OQM34_000178"
     CDS             71644..72294
                     /gene="hyfE"
                     /locus_tag="OQM34_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1450763.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            72299..73885
                     /gene="hyfF"
                     /locus_tag="OQM34_000179"
     CDS             72299..73885
                     /gene="hyfF"
                     /locus_tag="OQM34_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1450764.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAISWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            73875..75589
                     /gene="hyfG"
                     /locus_tag="OQM34_000180"
                     /pseudo
     CDS             73875..75589
                     /gene="hyfG"
                     /locus_tag="OQM34_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            75599..76144
                     /gene="hyfH"
                     /locus_tag="OQM34_000181"
     CDS             75599..76144
                     /gene="hyfH"
                     /locus_tag="OQM34_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1450765.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            76141..76899
                     /gene="hyfI"
                     /locus_tag="OQM34_000182"
     CDS             76141..76899
                     /gene="hyfI"
                     /locus_tag="OQM34_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1450766.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDLRLNSIVQQLFRVLRGLHD"
     gene            76892..77305
                     /gene="hyfJ"
                     /locus_tag="OQM34_000183"
     CDS             76892..77305
                     /gene="hyfJ"
                     /locus_tag="OQM34_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1450767.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            77335..79347
                     /gene="hyfR"
                     /locus_tag="OQM34_000184"
     CDS             77335..79347
                     /gene="hyfR"
                     /locus_tag="OQM34_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCS1450768.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SKQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            79369..80217
                     /locus_tag="OQM34_000185"
                     /pseudo
     CDS             79369..80217
                     /locus_tag="OQM34_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(80255..81316)
                     /locus_tag="OQM34_000186"
     CDS             complement(80255..81316)
                     /locus_tag="OQM34_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1450769.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            81529..82992
                     /gene="bepA"
                     /locus_tag="OQM34_000187"
     CDS             81529..82992
                     /gene="bepA"
                     /locus_tag="OQM34_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1450770.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            83013..83372
                     /gene="arsC"
                     /locus_tag="OQM34_000188"
     CDS             83013..83372
                     /gene="arsC"
                     /locus_tag="OQM34_000188"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1450771.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(83505..84251)
                     /gene="hda"
                     /locus_tag="OQM34_000189"
     CDS             complement(83505..84251)
                     /gene="hda"
                     /locus_tag="OQM34_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1450772.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(84301..85590)
                     /gene="uraA"
                     /locus_tag="OQM34_000190"
     CDS             complement(84301..85590)
                     /gene="uraA"
                     /locus_tag="OQM34_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1450773.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(85676..86302)
                     /gene="upp"
                     /locus_tag="OQM34_000191"
     CDS             complement(85676..86302)
                     /gene="upp"
                     /locus_tag="OQM34_000191"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1450774.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            86627..87664
                     /gene="purM"
                     /locus_tag="OQM34_000192"
     CDS             86627..87664
                     /gene="purM"
                     /locus_tag="OQM34_000192"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1450775.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            87664..88302
                     /gene="purN"
                     /locus_tag="OQM34_000193"
     CDS             87664..88302
                     /gene="purN"
                     /locus_tag="OQM34_000193"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1450776.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREASDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            88474..90540
                     /gene="ppk1"
                     /locus_tag="OQM34_000194"
     CDS             88474..90540
                     /gene="ppk1"
                     /locus_tag="OQM34_000194"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1450777.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            90545..92086
                     /gene="ppx"
                     /locus_tag="OQM34_000195"
     CDS             90545..92086
                     /gene="ppx"
                     /locus_tag="OQM34_000195"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1450778.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(92125..>92328)
                     /locus_tag="OQM34_000196"
     CDS             complement(92125..>92328)
                     /locus_tag="OQM34_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1450779.1"
                     /translation="LDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQG
                     YLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        22327 a        24115 c        23297 g        22589 t
ORIGIN      
        1 tgcgatactc aaagattcga acctgcagca ggtttgagtt gagcgcagcg aaacaacgga
       61 gccgctcgcg gcgacggccc gaagggcgag cgaagcgagt catcctgcac gacccaccaa
      121 tgcaaaaaag cgccctaaag gcgctttttt gctatctgcg atttgtaaga ttgatttttc
      181 ttctcaagtt ccttgttctt ctaccgatat tacctcgtac agtgacgagg actgaacaat
      241 ggccactatc tcttcaactt caattccttc catacaaaca cagagtagta acagggcatc
      301 acaaggtagc gatgttgctt cacaaatcgc gcggataagt cagcaaatca tcaaactcac
      361 tcaacaaatt aaagagattg tagacactag cggcagtgct gaagacaagc aaaaacaggc
      421 cgaattgatt caacagcaaa tcaccctact ggaaacacaa ctggcgcagt tgcagaaaca
      481 gcaggcagaa aaggcgcagg agaaagagca gcgtttgtct ctgaatgtat cccttctaaa
      541 tcctgttgaa aaaacgactc atatcgatat ctatatctga tacaacggat ccccttcccg
      601 accagccatt agcgcattgc cctggtctcg taccacgtcc cataacgctt cagcggcggt
      661 ggaaagtgaa cggttcttcc tgcgcaccag catcaactgg cgttcaacca ccggcgtaat
      721 gcgcttaacc accagtgggc taccttcagg caatggtagc gccagtgcag gaagaatact
      781 gatgccgatg cctgcggcta ccatcgggaa cagcgtcgcc ggatgtccaa tctcctgcac
      841 aatattcgcc tgaataccat tgcgcgccag cgctgcgtca atcagcggtc ggctgcctga
      901 cgcgtaatcc tgcaacacca atttcgctcc ctgtaatgcc tgccagggta cataatcttc
      961 cacggccaaa gcgctatcgc gatggcaaag cagaaagaaa ggctcggaaa gaatcgcttc
     1021 acattgcaga tcgcccactg ggcctggatc gatgacgatg ccaaagtcga catctccctg
     1081 gcgaatactt tccatcaccc actgctgcgg acgatcatgc aggacaaact gaatatctgg
     1141 atagcggcga tggctttcag caatgcattg cggaataaga tgcgcggaaa tggtctggct
     1201 ggcagcgacc cgaacttttc cgctcagttg ttgccccata cgcccggtat cgcgcaacgt
     1261 gctgttaagt tcatccagca gtcgctcaag acgcaaagcc aactgctgcc ctgcatctgt
     1321 aagcaccact tcacgcgtgg ttctgtccag caggcgaaca ccagtatgat tttccagctc
     1381 cttcacactg tgacttactg ccgactggct caagccgata cgctctcctg cacgactaaa
     1441 acttttctcc tgcgctactg tgacgaaaac ctttaattgt tttaaagaat aattcatctg
     1501 ttttcttcat gaatggatgc aataaatcaa ttttatttct caaactggaa gaagcacaat
     1561 agaaccatcg atcatctgga gtctttatga aactttttcg tatcctcgat cctttcacct
     1621 taaccctgat cacggtggtg ttgctggcct ctttctttcc ggccagaggc gatttcgtcc
     1681 ccttctttga aaatctgacc accgcagcta ttgccctgct gttctttatg cacggcgcga
     1741 agttgtcgcg tgaggcgatt attgctggcg gtggtcactg gcgactgcat ttgtgggtaa
     1801 tgtgcagcac cttcgtgctg tttccgattc tgggtgtact gtttgcctgg tggaaaccgg
     1861 taaatgtcga cccgatgctc tactccggtt ttctctactt gtgcattctc ccggctaccg
     1921 tgcagtctgc aatcgccttc acgtcaatgg cgggcggtaa cgtcgcggcg gcggtttgtt
     1981 ctgcgtcagc atccagcctg ctggggattt tcctttcacc attgctggtt ggtctggtga
     2041 tgaatgttca cggtgcaagg ggcagccttg agcaggtcgg taaaattatg ctgcaactgc
     2101 tgctgccgtt tgtgttgggg catctttccc ggccgtggat tggtgactgg gtgtcgcgca
     2161 ataaaaaatg gattgcgaaa actgaccaga cgtccattct gttggtggtt tatacagcgt
     2221 tcagcgaagc cgtcgttaat ggtatctggc ataaagttgg ctggggatca ttgctgttta
     2281 tcgtggtggt cagctgcgtt cttctggcta tcgtgattgt agttaacgtc tttatggcac
     2341 gccgactgag cttcaataag gcagatgaaa ttactatcgt cttttgtggt tcgaaaaaga
     2401 gtctggcaaa tggcatcccg atggcaaaca ttctgttccc cacatcggtg atcggtatga
     2461 tggtgctgcc cctgatgatt ttccatcaga tccaattgat ggtctgtgcg gtgctggcgc
     2521 gtcgatacaa acgccagacc gaacagttac aggcgcagca ggaaagcagc gccgataaag
     2581 cttaaagcgg acgcttcagg ggctggacca gttgcgtcag cccctcggtt ttaatcagca
     2641 gcgtgatttg cattagctca ccgagcttcc ctgccgggaa ctcatctttg cgggcaaacc
     2701 acagcagata ctcctccggc aggtcaatta agcgacgccc tttgtatttg ccaaacggca
     2761 ttatcgtatt ggctatttca atcagctgct ctttttccat ctcagctacc cagcaaacgc
     2821 agcatttccg cttcgtcgat gacttcaatg cccagttcct gcgccttcgc cagtttagat
     2881 cctgcagctt cacccgctat caccagatcg gttttcttcg acacgctgcc cgcgactttc
     2941 gcgcccagtt cgaccagtcg agctttagcg tcatcacgcg acatctggct taagctgccc
     3001 gtaagcacca cggttttacc aacaaacggg ctgtcaatct cttccgcgtt gataacgatc
     3061 ggcgcaggcc agtgaacacc ttccgccaac agctcgctga tgacattgcg gttgctttct
     3121 tcggcaaaga agttgtgaac gtgggatgca acgacaatgc caacatcagg caccttttgc
     3181 agctcttcaa tcgaagcggc ttccagcgct tccagcgtgc cgaaatatgc cgccagacct
     3241 gctgcggtgg cctcgccgac ttcacggatg ccaagtgcat agaggaagcg agcaaaggtg
     3301 gtttctttcg ctttttccag cgcgttaacc acgttttgtg ccaattttgg ccccatacgc
     3361 tccagtccgg tcagtttgcc tgcggtgagt ttgaacagat ctgccggagt atggacatat
     3421 tctttttcaa ccagctgatc gatgattttg tcgcccattc cgtcaacatc catcgcacgg
     3481 cgggaaacaa agtgtttcag cgactcttta cgctgcgcac cgcaaatcag gccaccggta
     3541 cagcgggcaa ccgcttcacc ttccacacgc tcaacgtcag aaccacatac cggacaatgc
     3601 gtcgggaata caacctcacg ggtatcttcc gggcgttcag aaagcacgac gttaaccacc
     3661 tgcgggatca cgtcgccagc gcggcgaatc accactttat cgccaatgcg taaaccaaga
     3721 cgttcgattt catccgcatt gtgtaaggtt gcgttactca ccagcacgcc agcaacatgg
     3781 acaggttcca gacgcgcaac aggcgtaatc gcgccagtac gcccaacctg aaactcgacg
     3841 tcacgcacaa aggtcatctg ctcctgcgcc gggaatttaa acgcaaccgc ccagcgcggg
     3901 gcacgcgcga caaagccaag ctgctcctgc tgttccagtg agttgacctt aatcaccacg
     3961 ccgtcgatat caaagcccag cgtcgggcgg tcttcttcca ctttgtgata gaacgccagc
     4021 acttcttccg ccgattcaca aagcgttacc cgatcgctga ccggcacccc ccacttttta
     4081 aattgcagta aacggccaag atgagtatcc ggcagctcgc caccttccag aacaccaacg
     4141 ccatagcaga aaaaagtgag cggtcgcttc gctgtaatac gcggatcaag ctgacgcagt
     4201 gaaccagctg ccgcattacg tgggttagca aaaactttcc cgcccgtgcg tcgcgcatct
     4261 tcgttaatct tttcgaaccc cgcctgcggc aggaacactt caccacgcac ttccagacgc
     4321 gccgggatat tctctccgtg cagcttcagc ggaatggcgc gaatagtacg cacattagac
     4381 gtgatatctt ccccggtggt gccatcgcca cgggtcgcgg cactgactaa aacgccattt
     4441 tcatacagaa tactgacggc aaggccatcc agcttcagct cacagcacca ggtgactttc
     4501 tcgttgtttt tcagacggtc ctgcacacgt ttgttgaaag caagaaagct ttcttcatca
     4561 aaaacgttat ccagtgacag cattggtact tcatggcgta tctggctgaa agctgccagc
     4621 ggcgcagcac ctacacgttg ggtaggcgaa tcaggcgtaa tcagttctgg atgtttggtt
     4681 tccagctcgc gcagttcgcg catcagcctg tcgtattcag cgtcgggaat ttccggcgca
     4741 tccatcacat gataaagata ttcatgatgg caaagcgtcg ttcgcagttc tgtcagttgt
     4801 tgttcgattg attccatatc gcaccatcaa tgctaaaaac ccccgacaag cgggggttcg
     4861 aagaggagtt aatttgcctt aagtgtatca ggcgttggcg tctttgactt cgcggatgat
     4921 gtcctggtac tcgcgcaatt tctgcggagt catcatacgg cgctgatcgt caagcacgac
     4981 accgcccact tcatcggcaa tatgctgcgc agattgcagc atcagcttga agttctgcag
     5041 ctcgtcaccg taagacggta cctgcataaa gatagtgaca cccggagtag tgaaatcctt
     5101 catttcagga tcaaaggttc ccggtttcac catattcgcc aggctgaata acgccgggcc
     5161 gctgccatcc gggctaagat gacgatggta aatattcata tcgccaaaaa tgaagcccgc
     5221 ttgttgaatg ctgttaagaa gcagttcacc gtttagctcg ctaccgtgat gcgccgcgac
     5281 gttcatgata atcaccgctt ctttgcgctt cggtttatcc ataactggag ccggttccgc
     5341 tacaggctca ggctgtggtg ccgctacggg ttctgcaggc tggaaagcct gttgtgccgg
     5401 ttgcggtgct gaatgcacag gctgcggcgc tggcgcgacc tgtggcgaaa ctggctgctg
     5461 caacggctgt tcaggctgcg gctgataggc aggctgctgc accggctgcg ctggacgcgg
     5521 agcatgttgc ggcggtacct gcgcttcagg cggctgctgg accggttgac gcggctgcgc
     5581 agacgcataa ggcggtaggt actggtgttg cggcgacgga cgagcagcct catgctcctg
     5641 agcgttagcc ggggcatgat tcacgcggtg aacacgaacc tcaccaacgc cctcatcatc
     5701 ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac ttcattcgtt ttaatggccg
     5761 atcgcggaac atagaagatc gttctttacg gctggtccag aaaccatgta ccagtaaagc
     5821 gattatggcg atcgcgccaa caatgattaa tatcagacgc aaatcctgca tcattatatt
     5881 ctctgttgtt ctaacacctt gccaccacgg caaacattta ctcactaaga gtatttgccg
     5941 attacctcaa gtgcaagtgc actattaact ttcacagcac aaagatagat gaaatcgtgc
     6001 tttttgctgt tttttcgaac atatcctaac tgtccattgc gcaattaccc ggtaaaatac
     6061 gcagaatttt cctgggattg gtcaaaagga gctcatcctg attatggttt catcattcac
     6121 atctgcccca cgcagcggtt tttactattt tgcgcaaggc tggaagctcg tctcgcaacc
     6181 tgggattcgg cgtttcgtta ttttaccgct gctggtcaat attttgttga tggggggcgc
     6241 attctggtgg ctctttacac agctcgatgt ctggatcccg gctttcatga gttacgttcc
     6301 ggactggcta caatggctga gttatctgtt gtggcctctg gctgtcatct ctgtgctgtt
     6361 agtgtttggc tatttcttct ccacgattgc taactggatt gccgctccgt ttaacggttt
     6421 attggctgaa caactggaag cacgattgac tggcgctaca ccgccagata ccgggatttt
     6481 cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa tggcaaaaat ttgtctggta
     6541 tctgccgcgc gcaattgtat tactaattct ttacttcatc cctggtattg ggcaaaccgt
     6601 cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta gccatccagt attgcgatta
     6661 ccccttcgat aaccacaaag tgccgtttaa agagatgcgc accgccctgc gcacacgcaa
     6721 aatcaccaat atgcagtttg gtgctttaac cagcctgttt acgatgatcc cgctgcttaa
     6781 tctgttcatt atgcccgttg ccgtttgtgg cgcgacggcg atgtgggtcg attgctatcg
     6841 cgataaacac gcgatgtggc ggtaacaatc taccggttat tttgtaaacc gtttgtgtga
     6901 aacaggggtg gcttatgccg ccccttattc catcttgcat gtcattattt cccttctgta
     6961 tatagatatg ctaaatcctt acttccgcat attctctgag cgggtatgct acctgttgta
     7021 tcccaatttc atacagttaa ggacaggcca tgagtaagat ttttgaagat aactcgctga
     7081 ctatcggtca cacgccgctg gttcgcctga atcgcatcgg taacggacgc attctggcga
     7141 aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg tatcggtgcc aacatgattt
     7201 gggatgccga aaagcgcggc gtgctgaaac caggcgttga actggttgaa ccgaccagcg
     7261 gtaataccgg gattgcactg gcctatgtag ctgccgctcg cggttacaaa ctcaccctga
     7321 ccatgccaga aaccatgagt attgaacgcc gcaagctgct gaaagcgtta ggtgcaaacc
     7381 tggtgctgac ggaaggtgct aaaggcatga aaggcgcaat ccaaaaagca gaagaaattg
     7441 tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt cagcaatccg gcaaaccctg
     7501 aaattcacga aaaaaccacc ggtccggaaa tctgggaaga taccgacggt caggttgatg
     7561 tatttattgc tggcgttggg actggcggta cgctgactgg cgtcagccgc tacattaaag
     7621 gcaccaaagg caagaccgat cttatctctg tcgccgttga gccaaccgat tctccagtta
     7681 tcgcccaggt gctggcaggt gaagagatta aacctggccc gcataaaatt cagggtattg
     7741 gcgctggttt tatcccggct aacctcgatc tcaagctggt cgataaagtc attggcatca
     7801 ccaatgaaga agccatttct accgcccgtc gtctaatgga agaagaaggt attcttgcag
     7861 gtatctcttc tggagcagct gttgccgcag cgttgaaact acaagaagat gaaagcttta
     7921 ccaacaagaa tattgtggtt attctaccat catcgggtga gcgttattta agcaccgcat
     7981 tgtttgccga tctcttcact gagaaagaat tgcaacagta atgccagctt gttaaaaatg
     8041 cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc ttcaaacttt cgcccctcct
     8101 ggcattgatt cagcctgtcg gaactggtat ttaaccagac taattatttt gatgcgcgaa
     8161 attaatcgtt acaggaaaag ccaaagctga atcgatttta tgatttggtt caattcttcc
     8221 tttagcggca taatgcttaa tgacgtacga aacgtcagcg gtcaacaccc gccagcaatg
     8281 gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac tggcgctaac aatacaggct
     8341 aaagtcgaac cgccaggcta gactttagtt ccacaacact aaacctataa gttggggaaa
     8401 tacaatgttc cagcaagaag ttaccattac cgctccgaac ggtctgcaca cccgccctgc
     8461 tgcccagttt gtaaaagaag ctaagggctt cacttctgaa attactgtga cttccaacgg
     8521 caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact ctgggcctga ctcaaggtac
     8581 cgttgtgact atctccgcag aaggcgaaga cgagcagaaa gcggttgaac atctggttaa
     8641 actgatggcg gaactcgagt aatttcccgg gttcttttaa aaatcagtca caagtaaggt
     8701 agggttatga tttcaggcat tttagcatcc ccgggtatcg ctttcggtaa agctctgctt
     8761 ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt ctgccgacca ggttgatcag
     8821 gaagttgaac gttttctgag cggtcgtgcc aaggcatcag cccagctgga aacgatcaaa
     8881 acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca tctttgaagg gcatattatg
     8941 ctgctcgaag atgaggagct ggagcaggaa atcatagccc tgattaaaga taaacacatg
     9001 acagctgacg cagctgctca tgaagttatc gaaggtcagg cttctgccct ggaagagctg
     9061 gatgatgaat acctgaaaga acgtgcggct gacgtacgtg atatcggtaa gcgcctgctg
     9121 cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca ttcaggatga agtcattctg
     9181 gttgccgctg acctgacgcc gtccgaaacc gcacagctga acctgaagaa ggtgctgggt
     9241 ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta tcatggcgcg ttctctggaa
     9301 ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg tgaaaaatga cgactatctg
     9361 attctggatg ccgtaaataa tcaggtttac gtcaatccaa ccaacgaagt tattgataaa
     9421 atgcgcgctg ttcaggagca agtggcttct gaaaaagcag agcttgctaa actgaaagat
     9481 ctgccagcta ttacgctgga cggtcaccag gtagaagtat gcgctaacat tggtacggtt
     9541 cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg ttggtctgta tcgtactgag
     9601 ttcctgttca tggaccgcga cgcgctgccc actgaagaag aacagtttgc tgcttacaaa
     9661 gcagtggctg aagcgtgtgg ctcgcaggcg gttatcgttc gtaccatgga catcggcggc
     9721 gacaaagagc tgccatacat gaacttcccg aaagaagaga acccgttcct cggctggcgc
     9781 gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg atcagctccg cgctatcctg
     9841 cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga tgatcatctc tgttgaagaa
     9901 gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg aactgcgcga cgaaggtaaa
     9961 gcgtttgacg agtcaattga aatcggcgta atggtggaaa caccggctgc cgcaacaatt
    10021 gcacgtcatt tagccaaaga agttgatttc tttagtatcg gcaccaatga tttaacgcag
    10081 tacactctgg cagttgaccg tggtaatgat atgatttcac acctttacca gccaatgtca
    10141 ccgtccgtgc tgaacttgat caagcaagtt attgatgctt ctcatgctga aggcaaatgg
    10201 actggcatgt gtggtgagct tgctggcgat gaacgtgcta cacttctgtt gctggggatg
    10261 ggtctggacg aattctctat gagcgccatt tctatcccgc gcattaagaa gattatccgt
    10321 aacacgaact tcgaagatgc gaaggtgtta gcagagcagg ctcttgctca accgacaacg
    10381 gacgagttaa tgacgctggt taacaagttc attgaagaaa aaacaatctg ctaatccacg
    10441 agatgcggcc caatttactg cttaggagaa gatcatgggt ttgttcgata aactgaaatc
    10501 tctggtttcc gacgacaaaa aggataccgg aactattgag atcattgctc cactctctgg
    10561 cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt gcggaaaaaa tcgttggtga
    10621 tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg cctgtagacg gtaccattgg
    10681 taaaatcttt gaaaccaatc acgcgttctc tatcgaatct gatagcggcg ttgaactgtt
    10741 cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa ggcttcaagc gtattgctga
    10801 agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa tttgatctgc cgctgctgga
    10861 agagaaagcc aagtctaccc tgactccggt tgttatctcc aacatggacg aaatcaaaga
    10921 actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc ccggttatcc gcatcaagaa
    10981 gtaattcttg ccgcagtgaa aaatggcgcc catcggcgcc attttttatg cttccgccag
    11041 cggcggcaaa atcaattcat cgctctcatg ctgctgggtg tagcgcatca cttccagtac
    11101 gcgcaacccc gcacggtgca ccgcatcgtt taacgccttc cctttcagca aaccactgat
    11161 aagctgagca caaaacaggt cgccagtccc tttcaggtcg gtttttaccc gtgaatggga
    11221 aatgacattc acgctgtcgg cactgaccac caccacctgc atctcctgat tttcttcatt
    11281 accggaggcg ctggtaatca ccacccattt taatgtgtct gaaagcagac tttttgcggc
    11341 agcaatggca ctgtcgagat cgcggcaatt tttaccggtc aggatttcca gctcaaagat
    11401 attgggggta attccctgcg ccagcggcag taaatattgt cgatacgcct cgggaaggtc
    11461 aggtttgaca taaattccgc tatcaatatc gccaatcacc ggatcgacca tgatcaatag
    11521 gtcaggatgg tctttgcgta gcgcagtcag ccactcggca aggattttga tttgcgatgc
    11581 cgttcccata tagcccgtgg ttacagcacg aagttggcgc agcgcatcac gctcctgaag
    11641 cgcacgcaaa tagccgctaa accattcgtc cggaatcgca ccaccgtaga aagtgtcata
    11701 atgcggcgta ttgctcagca ataccgtcgg cacggcaaag acattcaagc cgttctgttt
    11761 tatagcaggc accgcaatgc tgttgcctac gcttccgtac actacctgcg actgcacggc
    11821 gacgatatcc gcctgcagcg ccctactctt atcgttaaac aacaacaaac tactcattta
    11881 attttttctc cttgccgatg atcctcatcg tattccaacc gaaactttac ctgattctgg
    11941 cagtcaaatc ggctattaca aaacaaagat aaggtaattc agtgaagaaa atcatttgtc
    12001 tggccattac gctgctaatg acactccccg cttacgcgaa gttaactgtt catgaagaag
    12061 cccgcatcaa cgccatgctg gagggattag cacagaaaaa ggatttgata tttgtgcgca
    12121 acggtgatga acatacctgc gatgaagcgg tttcccacct gcgtctgaag ctcggcaata
    12181 cccgtaaccg cattgacact gccgagcagt ttattgataa ggtagcttcg tcgtcatcga
    12241 ttaccgggaa gccgtatatc gtgaagatcc ccggtaagag cgatgagaac gcacagcctt
    12301 ttttacatgc gttaattgcg cagacggata aaacggtgcc tgcggaagga aattaatccg
    12361 ctttggtaag gcatttacag gaggtaacat gaaaaaatgc tttatttatc acgatgaaaa
    12421 atcgaataaa ttttggtgga tagattacga aggggatagt ttagctgtca actatggcaa
    12481 ggtaggtagt attggtaaat tccatacaaa agaattcgat aatgaagaac agtgtctgaa
    12541 agaagccagt aaattgattg ccgcaaaaat gaaaaaagga tatcaagaaa ctccagactt
    12601 taacttcatg gatctctact attttgatga tgaagagatt gggttacatc ttaaaacgtc
    12661 acacccaaac ttccagtgcc attttactga tccactttat atgtgttgct gggatgaaga
    12721 atctcctttt ggtagcgatg aaggtgctga tgctctaaac gaacttgaaa atagcctccg
    12781 ttctggactg tgctgatttc cctcaaatgt taattgaaac tatgtggggt atgaaataca
    12841 tcgctatgga cagtattctt gaagaggatg tgcgtgcgca attactagcc aatgaaatga
    12901 gctctatcca gagcaatatg attacctacg cgactgcatt cggtcagatc aaagtcatgg
    12961 gtaaaatctc ccataaactt aaaaagatgg gactcaatgc actagcgcgt catcagctta
    13021 ccgcaaaaat tcttcaatgg ggtgacggtc aggactcacc aatacttcaa aaaatgattg
    13081 atgaccttac tgcttttcca cacgaaaatt aaaaactgca tttgtcggca gcaacaactg
    13141 ccgacaaatc agattattga caaagtgcac tttgtttatg ccggatgcgg cgtaaacgcc
    13201 tgatctggca taaaaatatt gctaattcaa tatattgcag aaacattgta ggcctgataa
    13261 gcggagcgca tcaggcagtt ttgcgtttgt catcagtctc caatgctatt aatccttaaa
    13321 tccctgcccc cagactaaaa tgctcttccc caaacactcc ggtggagagg tagcgatcgc
    13381 cacgatcgca gatgatcgct accaccaccg caccagggtt agcttttgcc acccgcagtg
    13441 ctccagcaac cgcgccgcct gagctgacgc cacagaatat tccttcccgc accgccagtt
    13501 cgcgcatggt gttttccgca tcgcgctgat gaatatccag cacctcatcc accagcgcag
    13561 cgttgaaaat ccccggcaga tattccgcag gccagcggcg aatgcccgga atactgctgc
    13621 cttcttccgg ttgcaggcca acaatggtca ccggtttgga ttgttcgcgc ataaagcatg
    13681 aaacgccggt gatagtgccg gtcgtcccca tgctggagac aaaatgagtg atgcgcccgc
    13741 cggtttgctg ccagatttcc ggcccggtgg tggtgtaatg cgcataaggg ttatcgggat
    13801 tattgaactg atcgagcagc tttccttcgc cacgattcgc catctccagc gccagatcgc
    13861 gcgcaccttc catgccctgc tctttggtga caagaatcag ttccgcacca taagcacgca
    13921 tcgccgcacg gcgttcctgg ctcatgttgt cgggcatcag caatttcatg cgatagcctt
    13981 tcagcgcggc aatcattgcc agcgcaatgc cggtgttacc actggtggct tcgattaaga
    14041 catcacccgg tttaatttcc ccgcgctttt ccgcctcgac gatcatcgaa agtgccgcac
    14101 gatctttcac cgaacctgcc gggttattgc cttccagttt taaccacact tcactgccgt
    14161 tatccggccc cattcgctgc aacttcacca gaggcgtatt gcctattgtt tgttctaatg
    14221 tactcacaat ctctatccat acgtggtgtt gcctgatgcg acgcttgcgc gtcttatcag
    14281 gcctacaggt tacaaacctt gccattaaaa agcccggatc gcgggaagcg cctccgggcg
    14341 tttaacattc actcaaccta tcaggcgctt tgtgcgagag caagttcctc atcgcgggtt
    14401 tcaatacgct cgtcgccgtt atacagccgc gcatgttgca gaccaacgaa taaacgctcg
    14461 ccatgctgcg gggcatcgtc gccatgcatc acgaccgtca gcggttcgtt gtaccacccc
    14521 agcggctgca ccactaattg ggtgtagtga cctttcgggc tggcttccag tacctgtacc
    14581 ggcagcggcg aatcgagact ggtacggcgg ctgatatcca cttcccacgg gcgcaggaag
    14641 agatccaccg gcccctgata cgcaggtgtg tagcccagcg gccagcgatg cgcgccaaca
    14701 tggaactgcc cgccgcgaat ggttccctgc aggcggttca cttcgcccat aaattcgagt
    14761 acaaaacggg tcgctggttc gcgccatacc tgattcggcg catcagcctg ttcaatattc
    14821 ccctgactca tcaccactac acgatcggct acttcggtcg cctcttcctg gtcgtgggtc
    14881 acaaacacgc tggtgaattt cagctcttca tgcagttgac gcagccagcg acgcagctct
    14941 ttacgcacct gcgcatccag cgcgccgaac ggttcatcaa gcagcagaat ttgcggttct
    15001 acagccagcg cgcgcgccag cgccacacgc tgtttctggc cgccggaaag ctgcgccgga
    15061 taacgatccg ccagatgcgc aagctggacc atttccagca attttgtcac tttcgctttg
    15121 atggctgcgg cattcgggcg ctcgcgacgc ggcagcaccg tcaggccaaa agtgatattg
    15181 tcgaataccg tcatatggcg gaacagcgcg taatgctgga acacgaaacc gactttacga
    15241 tcgcgcgcgt gcaggcgact cacgtcggtg ccgtggaagc gaatatgccc gctggtttga
    15301 tgctccagcc cggcgataat ccgcagcagc gtggttttcc cggaaccgga cggccccagc
    15361 aacgcgacca tctgaccaga aggaatatcc agtgagatat cgttcagcac ctgggtgcga
    15421 ccaaaagact tcttgatatt ggcaatctca atgctcatga tgctcctcct gctgcgcgcg
    15481 tttttcctga ttctccaggc gccactgcaa catactcttt aaaaacaggg tgataatcgc
    15541 catcaacgtc aacagcgcgg cggcggtaaa ggagccgacg gtgttgtagt cctgctccaa
    15601 caattcaatc tgtaacggca gcgaaagtgt ttcgccgcga atcgagccgg aaaccaccga
    15661 cactgcacca aactcgccaa tcgcgcgggc gttggtcagc accacgccgt aaagcagcgc
    15721 ccagcgaata ttcggcaatg tgacgcgacg gaacatctgc cagccggacg cgccaagcaa
    15781 aatcgccgct tcgtcttcct ggctgccctg gcttaacatc accggcacca gctcgcgcac
    15841 cacaaacgga cacgtcacaa agatggtgac cagcaccatt ccgggccagg agaacataat
    15901 ttgcagatta tgttcatcca gccagccgcc gagcgggccg ttggagccgt agaacagcag
    15961 atacaccaga ccggcaacca ccggcgatac agcaaacgga atgtccagta gcgtcagcag
    16021 taactggcgt cccgggaagt taaagcgcgt caccagccag gccagcagaa tgccgaacac
    16081 caggtttacc ggtacggcaa tcagcgcgat catcaccgtc agccagatgg cgtgcagcat
    16141 gtccggatcg gccagattct gtaaaaccgg catcagcccc ttgctgaatg cctgcacgaa
    16201 gatgtaaatc atcggcacca gcaggatgaa cgccgaaacc agcatcccga tgccaatcag
    16261 aaaccatttg ccccagttaa tcgggcgcgc gtcataacgc ttcaattggg taacttccgc
    16321 cattaatgac ctaccacacg ccgaccaaag cgactttgca gagtgttaat tgagaacagc
    16381 agcagcagag atgccgcgag gatcaccgaa gcaatcgcgc tcgctgccgg gtaatcaaac
    16441 tcctgtaagc gcacaaaaat catcagcgac gtcacttccg tcttccacgc gatatttccg
    16501 gcgataaaaa tcaccgcgcc aaattcacca agactacggg taaacgacag cgtcacgccc
    16561 gccaccagcg ccggagaaag ctccggcagc accactttgc agaaactctg ccagcgcgtt
    16621 gcaccaagcg tttccgccgc ttcttcatat tccgggccta actcttccag caccggctgc
    16681 acggtacgca ccacaaacgg aatgctggta aaggccatcg ccaccgcaat accaagccag
    16741 gtataggtga ctttgatatc aaacttcgcc agccattcac cgtaaaaacc gtttacggaa
    16801 aagagcgagg ccagcgttaa accggcgact gccgttggca gcgcaaaggg taaatccatc
    16861 agcgcatcaa gcagcgtgcg gcccggaaag cgataacgtg ttaaaatcca cgccatcagc
    16921 agaccgaaaa cgccgttaaa aatcgatgcc acaaacgccg acagcagcgt cactttgtag
    16981 gccgcgacca cctgcgggtt ggtgatcacc tcccagtact gcgcccagct catctcagaa
    17041 agctgcatca ccagcgcaga gagcggcagc agcaatatca ggcacacaaa cagcagactg
    17101 gtgccaaggc ttaaagtaaa gcccggcagc acgcgtcttg aagaaactgc aaacattact
    17161 tacgccccgc cgctaacagc ttgtctaact cgccgccgct ggtgaagtgg gttttcatca
    17221 cttccggcca tgagccaaat ttgtcttcca cgcggaacag ttcggtctgc gggaatttat
    17281 ctttcagttt gtccatgact tccgggttat tcacacggta gtaatagtcg gtgatgatgg
    17341 tctgcgcctg tgggctgtag agccagttca ggtaggcttt tgcggctttt tccgtaccgt
    17401 tggcctgcac atttttatcg acccacgcca ccgggaattc cgccagaata ttggttttcg
    17461 ggatcaccac ttcaaagccc tgtgcttcat actgtttacg gatgttgttc acttccgatt
    17521 caaagctaat cagcacatcg cccaagccac gctcggcaaa agtggtggtt gcgccacgac
    17581 cgccggtatc gaacacttca acgtttttca ggaactgggt cataaactgt tcggttttgg
    17641 ctttatcgcc accgtcagct ttatccgctg cgccccatgc cgccagatag gtataacgcg
    17701 cgttacccga cgttttcggg ttcgggaaaa tcagcttcac gtcggagcgc accaggtcgt
    17761 tccagtcgtg gatattcttc gggttgccct tacgcaccag gaagcccatg gtggagtaga
    17821 acggcgagct attattcggc aggcgcgact gccagtcagc cgggatcagc ttgcctttgt
    17881 cgtgcaggat ttgtacgtcg gtcacctggt tataagtgac aacgtctgct tttaagccct
    17941 gcaaaatcgc cagcgcctgt tttgatgacc cggcatgaga ttgttttatc gtcagtttgt
    18001 cgccgccgtt atcttttgcc cattgctgtt caaacggcgg attcagggcg gcaaacagct
    18061 cgcgggagac gtcataagaa ctgttcagca gttccgttgc ctgtacatgg cccgccagca
    18121 gcagagaagc gaccagcgcg agtgagttct ttttcagtaa gttaacggcc attgcgcacc
    18181 cttataaatt taatgacttt ctaatagcca tcatatttat aacggacgtg aaaggagtaa
    18241 cggttttata taccgtttgg tgatttggaa gttaaaaagg gaataagaac ttggttcaac
    18301 aggcgtaatt gttgcaggca gtttggacac ggacagcgcg gagaaaccgg agcgtacaca
    18361 tagtacgtga ggatttcgag cactgcccgg ggccaaaatg acaaatgaaa tagcctggtg
    18421 aacttagttc aagacgaaat cctccccaca catgcaggga ggaaacagag gtgaatcaga
    18481 taccgacgct aaccgtctcc ggcagtgtgc tgccgccatc aatcacattc tgtgtaccgg
    18541 ttaaatagct ggattcatcc gatgcgagga aggccgccag ttcgccgact tccagcggat
    18601 cggcgaggcg acacagcggg attgcttttg ccatttcagt cagcaccgat tctggatctt
    18661 ccgggttcga ctggcgggca atgctttccg ccattggcgt gcggacgtat cccgggcaga
    18721 tggcgttaac gcgaataccg gattgcgcgt actccaccgc cagcgattta gtcaggccaa
    18781 caatcgccgc tttcgttaag gcatacgccg tttcgccagg atcggccacc atatcaccag
    18841 tgactgaaga catcatcaca atgcgaccat ctttgcgcgc aatcatctcc ggcagcaccg
    18901 ccttcgtgac gttccatacg cctttaatat tgatatcaat atggaaatcg cgatcttcat
    18961 cgctcatatc gaggaaactg cccagacgac aaacgcctgc gttattcacc aggatatcaa
    19021 tgcgcccttc tttttccttc gcgcgtttga tagctgcggc taccgacgcc gggtcacgca
    19081 catcggcgac aaccgccgta cagcgatgac cacgaccaca cagttcgtcc gctagctttt
    19141 cgatctcagg ggagatatcc agcaagatta ggttcgcgcc atgacgtgca aaagttctgg
    19201 caattccttc gccaattccc tgcaatgcgc ccgtaatcag tgctgtcttg cccgtgagtt
    19261 tacccatttt aatgctctcc ttgttaatac agagcgttac actccattcc ctctgaaaat
    19321 acaggataaa caaaatattg ctgcggatca gctcactaat tcaggaaaat ctcaatccag
    19381 tcgctgcttt atgcatcatt tcaggcgttg tgttagctca ctgctgcgct gaatcatttt
    19441 caatgacgcc acatcattga tgttgcacaa gaccaacaaa cagtaaatcg gtaacagaat
    19501 tttgcgccgt gcgggtagac atcgacgagc tacgccattc ggtttcaccg gaaacggtat
    19561 cgagcgtgaa atgcgccagc cgccgcagcg gagagtcagc cagagaagta atggcaatca
    19621 ccgttgcccc ctgctttcgt gccgcttctg cgcacaagac aatctctttt ttactgccgc
    19681 tgtaagagat agcgatttgt acatcgcctt ttttcaacgc ctgggaaacg gtggcttgta
    19741 cgtgcgtgtc ggcttcgcag gccacgcgat agccaatttt catcagtttg aacgacagat
    19801 cgcgccccac cagcgccgat ccgcccaggc ccgttatctg gataaacggt gctttactga
    19861 tgacctcaat gattttttgc agccgcgcgt agtcgaacaa cgcacaggtt tgctccagcg
    19921 ccagctcttt ttcgcgattc agtttgcggg caatcacttc cagcgaatca tcactggtaa
    19981 tcgaactgtg caggtgcagc gccgtggcat tcgttttctc ccggctggca ctgtattcgc
    20041 caattaacgc catacgcaat tcagtaaatc cttgcgcgcc gagcttctgg gcaaatttca
    20101 caatgctcga ctggctgata ccgagctgtt tcgccatctg ccgtgaagag acggatttca
    20161 gctcgctcac tctggcccgc agaaagtcag caattttttg ctcgttcccg gtaaattcac
    20221 tttccgcatt gcgtattttc gtcaggtaca acatcttcca ctctcctggg ctggcagcaa
    20281 aaattccgct taagttctta tgacgctctt cacactctgc gagtgtagac tcaatgattc
    20341 ctttaagtct gtcaattcgg gatatatgat tccatacatc tgcaattatt tgatcgtaaa
    20401 tagtaaggtc accaccgatg caactagaaa agatgattac tgaaggctcg aacaccgcct
    20461 cggctgaaat tgaccgcgta tcgacgctgg aaatgtgccg gattatcaac gatgaagata
    20521 aaaccgtacc gcctgccgtt gatcgcgtcc tgccggatat cgccgcggcg atcgatgtta
    20581 tccacgccca ggtcagcggc ggcggtcgtc tgatttatct cggtgcggga acatccggtc
    20641 gtctggggat tctggatgcc agcgaatgtc cgcccaccta cggcgtgaaa ccgggtctgg
    20701 tggttggttt gattgctgga ggcgaatatg ccattcagca cgcggtggaa ggagctgaag
    20761 atagccggga aggtggtgtt aacgatctga aaaatattaa tttaacggca caggatgtgg
    20821 tggttggcat tgctgccagc ggtcgcacgc cgtatgtgat tgccggactg gaatacgcac
    20881 gccagctcgg ctgccgcaca gtgggaattt cctgtaatcc ggggagcgcc gtttcaacca
    20941 ccgctgagtt tgccattaca ccgattgtag gtgccgaagt tgttaccggt tcttcgcgga
    21001 tgaaagcagg tacagcgcag aaactggtgc tcaatatgct ttccaccggg ctgatgatta
    21061 aatccggcaa agtgttcggc aacctgatgg tcgatgtggt cgccaccaac gaaaaactgc
    21121 atgtgcgcca ggtcaatatc gttaaaaacg ccaccggatg taatgccgaa caagcggaag
    21181 cggcgttaat tgcctgcgag cgcaactgta aaacggccat tgtgatggtg ctgaaaaatc
    21241 tcgatgccgc agaagctaaa aaacgcctgg atcaacacgg cggctttatt cgtcaggttt
    21301 tagacaagga ataacccatg gccaaagaga tcagcagtga acttctgaac accattctta
    21361 cccgtgtcgg cggaccagga aatatcgcca gttgtggtaa ctgtatgacg cgcctgtgtc
    21421 tgggtgtaca tgacagttca ctggttgatc ccaatatcaa aacgctggaa ggcgtgaagg
    21481 gcgtcatttt gaccagcgac caggtgcagg tcgtttttgg gcctggtaaa gcgcatcgtg
    21541 ccgcaaaagc gatgagcgag ctgctgggcg atgcaccggt acaggatgcc gcagaaatcg
    21601 ccgcccagaa caaacgtcag ttaaaagcca aacaaacctc cggcgtgcaa caatttctcg
    21661 ccaaattcgc caccatcttc acgccgctga tccccggttt tattgccgcc ggtctgctgc
    21721 tggggatagc gacgttaatt gccacggtga tgcacgttcc ggcagatgct cagggcacgc
    21781 tacccgacgc gctgaatttt atgaaggtgt tcagcaaagg tttgttcacc ttcctagtga
    21841 ttctggtggg ctataacgcc gcccaggcat ttggcggcac gggcgtcaat ggcgcaatta
    21901 ttgccgcact ctttttgctc ggttataacc ccgctgcaac caccggttac tacgccggtt
    21961 ttcacgattt ctttggtctg cccatcgatc cgcgcggcaa tattatcggc gtgctgattg
    22021 ccgcgtgggc ctgcgcccgc atttaaggca tggtgcgccg ctttatgccg gacgatctcg
    22081 acatgctgct gacctcgtta atcaccctgc tgatcaccgc tacgcttgcg tacctgatca
    22141 ttatgccgct gggtggctgg ttattcgaag gtatgtcatg gctgtttatg cacctgaaca
    22201 gtaatccgtt cggttgtgcg gttttagccg ggctgttcct gatcgccgtg gtatttggcg
    22261 tgcatcaggg ctttattcct gtttacctcg cgttaatgga cagccaggga ttcaacagct
    22321 tatttcccat cctttcaatg gcaggcgcgg gccaggtggg cgcggcgctg gcactctact
    22381 ggcgggcgca accgcacagt gcgctacgca gtcaggtacg cggggcgatt attcccggcc
    22441 tgctgggcgt tggtgaaccg ctgatttacg gtgtcaccct gccccgcatg aagccgtttg
    22501 ttaccgcctg tttaggcggc gcggcgggcg gtttgtttat cggattgata gcctggtggg
    22561 gtctgccgat gggcttaaac agcgcctttg ggccgtctgg tctggtggcg ctgccgctga
    22621 tgacttccgc acaaggcatc cttccggcaa tggcggttta tgctggcggg attctggtgg
    22681 catgggtttg cgggtttatt ttcaccacgc tctttggctg ccgtaacgtc aatctggact
    22741 gatattatga aacggacaat gctctatctt tctctgctgg ctgttagttg tagcgtcagc
    22801 gccgcaaaat accctgttct gacagaaggt agttgatcaa gtatatgcgg cgttaaagca
    22861 gtaataaaaa ggccagtcag taatgttact gcctggcctt taatcaatga atcagaaacg
    22921 cttacagcgc catcaacttg tccagcgacg gtgcgaaata atagccgccg gttaccggtt
    22981 tggtgaaacg caacatcgca tcacgtttgc catcggtatc gccaaacatg ctcagcagtt
    23041 gctgctcaat gttatgcaga cgcgcgcagt aggcgcagaa gtacagaccg tgagtgccac
    23101 tggcggtgcc gtacggcagg ctctggcgaa caatcttcag ccctttgcca tcttctttca
    23161 gatcaacgcg ggtgaggtga gaggtttccg gacgttcgtc gccgtcaatc tcttcgttgg
    23221 cctctttggt gcgcccgatc atcatctcct gatcgtgaac gctcatccgg ttgagctgct
    23281 tcaggttgtg ctcccaacgc tgcacaaaca catagctgcc gccggaatcc acgccgtctt
    23341 tgataaccgc cacttcgcgg cgcgtctctt cacccgccgg gttttctgta ccgtcaacaa
    23401 agccgctcag gtcacgctct tccacccaac ggaagccgtg gatctcttct ttcacttcaa
    23461 tgcagtcacc aaaggcttcc atcgccgcct gagcgacaga gaagtttacg tcgtgacgca
    23521 gagaaagaat gtggatcaac acatcgaact gggtcgtcgg cgcaaggcct ttaccgtagc
    23581 ccggaaaatc tttcagctct tctgccccaa cgccgccgct cagagcgcgc caggtgttgt
    23641 taccaaaggc aaccaccgca ccaagatgcg cgtccgggaa tttcgcttca aaagttgcca
    23701 gtttgtcggc aaatgtttta ctggccgcac gcagggcgtc aacttcccct ttcacgttgg
    23761 cttcgatcca aatcgccgcg cggcaatgtt ctggcaaaat gccactctga acctgagaca
    23821 ttgttcctcc tgaaaataag aatgccacgc aatccgtggc attgttgagc gctattttac
    23881 gtgttttttc gcgacggcgg tttgttcaga cgcaaattaa cgccgccaga taattttact
    23941 gactttccag tttttcaggg tatcgtccga aggcattaac ccttcaggac cacgccattc
    24001 cccgctgaac tggtagctaa tatgttgact gccttcggct ttacattcga cggcacgatt
    24061 atcatcacca tcagcttttt ggcaattgcc aaatgctttg ctgtaaaggt cgctaaacgg
    24121 tgtaccgatt ttaacaccgg tgtcagcagg aatatcgcta tccagcacgt caatgcgact
    24181 gatcgtgcct tgatcgccgt taatcaccat cgccacgttg tcgcctttca tcacttcaaa
    24241 aaagcgcacc acgttgccgt tcgcggtttt cattccgctg cgcaggcgat aatcgccatc
    24301 aagcgcatca gcaatggctt gttcttgcag tggtgtggac gccgttaatt cacccacgcc
    24361 ctgctcgctc actttggtgg acgatccaaa ccagttccac gggttagcgg cagaccagtt
    24421 aactgacgac atcgtggaac agccggtcag catcaacggc atagcgcata acattaaacg
    24481 cagcgatttc atgtcacttc ctttggttat tcaataacgt tgcttggagt gcaaattcac
    24541 caaaaagtgc cgttattctt tttcttcatt aaaacaagcg cgtaaacgtc gattggtcag
    24601 cagccagatt aacgccacaa tatccgccac taccagcgcc agaccgatac cgctaacgga
    24661 ttcaccgttc agccacagcc acggttgcca gcaaagtaaa accacctgcg ccaacagcaa
    24721 cagaaaatag agcacatgcc aggtgcgagg aaacgaagcc cgccgaccgc tcaacaaaaa
    24781 cgccagcacc gccggaatac cgggaatcag ccccagccag aaattatcgt gatcgggata
    24841 aaacagattt agcagcgcag taccctgctc acgcgacgca ccggcaatga caaacagcac
    24901 ccaggttcgc gcctgaagca atagcacaag ccagaagagc aagggtaaac gcaggcgtcc
    24961 gtgcgcatca taatggacag gatggaactc agtactcttc atcttcaatc aaacgcttac
    25021 ccagactcag cacgtcggcg tgttcatatc ccaggcgttc atacattccg agcaccatgt
    25081 cgttatcttc cggcacgttg atctgaattt tcgggcagcc gcgagcaatc agctttttct
    25141 ccagccggtt aagcaacgca ttggcaattc cacgcccacg aaactctgga tgcacgccaa
    25201 gataataagc agacccgcga tgcccgtcat aaccgcccat caccgttccg accacttcac
    25261 cgtttacctc agcgaccaaa aacaaactga cgtcatggtt catcttacgc tcgatgtcca
    25321 tttccggatc gttccacgga cgcagcaagt cgcaacgctc ccaaagggtg atgacctctt
    25381 cgaaatcttc ctggcgaaat acgcgtatct ccatggtatt cgttaccttt ttgcgggtta
    25441 aaaggctgat tatggcgtga acggtcgaat tagccaatat ctgacgaaaa tcggttgaaa
    25501 aagtggcata atggggagtt gtcaactatt gaaatgaaaa gtaaaacaat tctcaacagc
    25561 aaaccgtcgt aacggattac gcgatacgat ataacatctg gaactttatt attacaactc
    25621 aggccgtatg agcactttta aaccactaaa aacactcact tcgcgccgcc aggtgctgaa
    25681 agccggttta gctgccctga cgttgtcagg aatgtcgcaa gccattgcca aagacgaacc
    25741 tttaaaaacc agcaacggac acagcaagcc gaaagccaaa aaatctggcg gcaaacgtgt
    25801 cgttgttctc gatccaggcc acggcggaat tgataccgga gcgatcggac gcaacggttc
    25861 gaaagaaaaa catgtggtgc tggcgattgc taaaaacgtc cgttccattt tgcgtaatca
    25921 tgggattgat gcgcgtttaa cgcgttctgg cgatacgttt atcccacttt acgatcgcgt
    25981 tgaaatcgcc cataaacatg gcgcagatct gtttatgtca attcatgccg atggctttac
    26041 caacccgaaa gctgccggtg cttcggtatt tgccctctct aaccgtgggg caagtagcgc
    26101 aatggcgaaa tacctgtctg aacgcgaaaa ccgcgccgat gaagttgccg gtaaaaaggc
    26161 gactgacaag gatcacctat tgcagcaagt actgtttgat ctggtgcaaa ccgatactat
    26221 taaaaacagc ctgacgcttg gctcgcatat tctgaaaaag attaagccgg tgcataaact
    26281 gcacagccgc aacaccgaac aagccgcgtt tgtggtgttg aaatcgccgt cggttccttc
    26341 ggtgctggtg gaaacctcgt ttatcaccaa cccggaagaa gagcggctgt taggcacggc
    26401 ggcgtttcgt cagaaaatcg ccacagcgat tgctgaaggc gtgatcagtt atttccactg
    26461 gttcgacaac cagaaagcac attcgaaaaa gcgataagtt atgaaacccg acgcacacca
    26521 ggttaaacag tttctgctca accttcagga tacgatttgt cagcagctga ccgccgtcga
    26581 tggcgcagaa tttgtcgaag atagttggca gcgcgaagct ggcggcggcg ggcgcagtcg
    26641 ggtgttgcgt aatggtggtg ttttcgaaca ggcgggcgtc aacttttctc acgtccacgg
    26701 tgaggcgatg cctgcttccg ccaccgctca tcgcccggaa cttgccgggc gcagtttcga
    26761 ggcgatgggc gtttcactgg tagtgcatcc gcataacccg tatgttccca ccagccacgc
    26821 gaatgtgcgt ttttttattg ccgaaaaacc gggtgcagag cccgtttggt ggtttggcgg
    26881 cggcttcgat ttaacccctt tctatggttt tgaagaagac gccattcact ggcaccgcac
    26941 cgcccgtgac ctgtgccagc catttggtga agacgtttat ccccgttaca aaaagtggtg
    27001 cgacgattac ttctacctca aacatcgcaa cgaacagcgc ggcattggcg ggctgttctt
    27061 tgatgatctg aacacgccag atttcgacca ctgttttgcc tttatgcagg cggtaggcaa
    27121 aggctacacc gacgcttatt taccaattgt agagcgacgt aaagcgatgg cctacggcga
    27181 gcgcgagcgc aattttcagc tctaccgtcg cggtcgttat gtcgagttca atctggtctg
    27241 ggatcgcggc acgctgtttg gcctgcaaac tggcgggcgc accgagtcga tcctgatgtc
    27301 aatgccgcca ctggtacgct gggagtatga ttatcagcca aaagatggca gcccagaagc
    27361 ggcgttaagt gagtttatta aggtcaggga ttgggtgtaa ctccctcacc cccattcccg
    27421 catccgctga tgcaacgtca acgacggctt ctcggcaaac agctgctggt aatccgtggc
    27481 aaattgcccc agatgccaga atccccactg catggcggcg tcttttaccg ttgtgctttg
    27541 cgaccacggg cttatcagtt cgcggcgtac ggcgttcagg cgaatgcgtt tcagccacgc
    27601 gtttgggcca atgcctaaaa tagcgtgaaa cgcgttttgt agcgtgcggc gactgacatg
    27661 cagttgatta cacaaatcca gcaccgtcac cggttcggac atgttttcca gcacatattc
    27721 acgggcgcgg gaaagcaacc gacggtaact ctgatgactg atgctttccg ccgtcaccat
    27781 tggttgcgct tcttccagca tcgcccccat cgccattagc aaattatccc ccagcacttt
    27841 tcgcactgca ggctgatgga gattttccgg gttctcgcaa aacgtcgcca gcgcctgttg
    27901 gacaaagccc cacagcgcgg ctttatgctg ctctttcact tccagcgccg actggctacg
    27961 cagcatatgt aatacccgat ccggattgtg caaaaagtta gcctgacagg tgatgacatc
    28021 ttcagacagc actacgccca ggatcgtata atcgtccggc gtacttaatt caaactcggt
    28081 tccaccgggg cgcgtcgcta tttctgcgct tcccagacat tgcgaaccga taaatccctg
    28141 ctcaccgcgc gtcgccggaa tgccaaacca gaacgagttc ggccagacca ggcacgactg
    28201 acgcagcgcc agaccggtgt attcacgaaa aacctgaata tcatcgagta gaatttccgt
    28261 aaattcacca tgaaacttgc ccggatgcag ctgatcgtaa atctgctgct aggcggtaat
    28321 cgtcaaagca tgttcataga catccgttgt ctgtcgttga tgaacattat ccacttcgac
    28381 cttcggcgtg agcttcaggt tttcgggtaa gggttcatga taaagatggt gcaaattggc
    28441 tgtacgggtc tttttcatga tgttaatgcc gggtgttgta ggacacccgg cacctccgac
    28501 aggttaatgg ggcttgagac gataacgact actgcgttta cgtaacgtcc cggcagaaaa
    28561 gagctgttcg agcgcatttc tggctttttc cagcggccag ccaaagtggg cggccacttc
    28621 tcctgccgtc attccctgac atactgatgt taacagcgcc aacaactcgt cagcagattc
    28681 cgcgacgata actggcgcgt cgggttcctt tacgggttgc ttcggctggc ggttaaagcc
    28741 agtaaccagc cactgggtgt catcgtctgg ccgaccgatc tccttacggc tgatgacacg
    28801 cccggtacgc atcgcggcag cacaacctgc gtccagcgcc gcacgacacg ccgccagatc
    28861 gccttccacc accagcgtta agcgaccagg gtcaagcact tcgtgactga gcagacgaac
    28921 gttagctgct ttgagcatgg catccgctgc atctatcgcg gcgaccatgc cgtccacttc
    28981 cagcaatccc agtgcattga tcatcggcaa cccccgttac gcacgctgga ttggattacg
    29041 cgcgatttcc agcactgcat cggtaaaggc gttacaggct gctttacacg cggcctggct
    29101 accggttaaa aatgctgccg agtagttggt ttcagacggt ggcgggacgt aggtcaccag
    29161 ctgtacgtcg gcagatttca acgcggcatc aatgccgtag gtcgcttcca gcggcggtgc
    29221 caccagatac gccatcggat cgccaagcgt gatcccggcg gttgatgaaa gataagaacc
    29281 ggtacgcgaa accacatgcg ccaggaaggc ggtgttttct gcgtcattgg cccactggaa
    29341 agctgcgcca ttttcaatat gtgcaaccat cgcatccaga ccggcacgca cttccgccgg
    29401 gtttggccca ccaagcataa tcagcacttc accggcagtc ggtgacgggc cgtgtgccgc
    29461 tcccgcatac agcgagcgac catataccac ttcgaccatc gcttgtttgg tcgcttcgtc
    29521 agccgcaatg taggttacgt cgtcagaatc ggcagaaatc agcccgaggc tacgaatatg
    29581 cggcggcaat tttagttcac gcgcaaattc agcgttaacg gaggcaatca cccgcatggc
    29641 ggttaccgac ggtcgaatca aatctaaagc tggcatgatg cctccttatc gggtcatgtt
    29701 gatgccggat gctttctgct ccagcatacg tttcgccaaa tccacaatga cggcacagca
    29761 gcacgaaaga gaagcaaaag tcagctacct ggtacactag gcagcgggtc agcaaagggg
    29821 ggcaaatgcc ccttttttca tgtcagataa tcagatcatt acctcaataa actaataatc
    29881 acatcgcggt tacaaccagt ttgatagtga tagctcaaac ccagacgtgg cgctggctgc
    29941 aagcaaaata agccgcaaaa atgcgacccc atgtttacta gccgcaaaaa tgcggtccta
    30001 tttgccagaa ccttttatta ttcaattggt tagtgcaaga aaaagcgtga ttgctgttca
    30061 agaagtatcc agaaaggtca tgattttgac gctgaaaaaa aataggtggg catttttgcc
    30121 cactcactag ccgcaaattt gcccactcac tagccgcatt tttgcggccc aaagtaataa
    30181 atacagagaa ataataaata cagatcagcg ccaagatttt tcgtcttttc ggcggaatca
    30241 tgagactagt gcttacgtcc tgataacctc cagagcagac cttaacttct gtgcagcagg
    30301 actacgctca ttgccgctcg gctaggtcaa atcctcacga taaagctgtt cggattgtga
    30361 ccataatgtt aaaggactct ttaaatcatt ccttgacgaa ttaactagct ggcttaagcg
    30421 gaatagcatg caggataaaa acaaaaccag cttagcagtg gggagagctt cgcttcgctc
    30481 gtctcctggg gccaatgctc aatgaaacga cgtaaggcac ggagcgctca ccataggtag
    30541 acacataaac atctgagagc gcaccttcta tcgcgctacg cgctcaataa aaaccaacga
    30601 caccgccaca ctgcaagacc aatggttttt atcgcgtata cagaccagac aggaaacagc
    30661 aacaaagcag cgtatagcca ggtgcagaca gcacactcac acgacgcaca gcaggcttaa
    30721 gtgatcactc aggaaacttt atccccgccg ccgatcaccg cgaactatca tgaccgcgcc
    30781 aaattcgaaa ggaatggccc atagaacgcg atgaacacac ataagcgcta cacttactta
    30841 acgtcgccac agagcgattg agagcgctta aaatcgatgc gatttcatta aatttttctc
    30901 actcgcctga atggtggcga tcaggactga tcgcaacatt gccccgcctc gatattgagc
    30961 gcataataga tacatacatt aagcggaaaa gagataagtt tattgtgaaa atcagggggt
    31021 tacatatcat gcgcatatca gaacaacaac ggcagatcct cgactatctg gcaaaaaacg
    31081 aatcaatcac cgcacgacaa gccgcagatc tcgtttatgg tggcaatgtt acccgcgtcc
    31141 aggttgacgc cgcccgccgc tcactcatga cgatggttag taatgggcta atcaggaaac
    31201 aaggccgcgc tttcgtagct gaaagacata tcaatcacgc ttatagccag gacgttgaag
    31261 aattgcgccg ccagatcctt tgggtattca gcaaacaggg tcaggagtat ttcctttctg
    31321 aacacccaaa tttcgccgcc aaagagttaa cagtaagcgg attgtcgtgc gcactggatt
    31381 atcttgtcgg tgaaggttgc gttacttaca agcagctatg gcacgccctg aatacgcttg
    31441 tagaagatgg tttactcatt gccacgcgtg agaacatcga atactggata atgccgcgtt
    31501 caggtcgccg caatcatgca cggtatattt caggcaacag atacagaacg ccggacttta
    31561 atccgacaca ggaagatcgg gaccgtgccg aaatgatgtg cagggaacag gagaaaagaa
    31621 ttatggataa gttattttcg atgcagtgaa cacaagcggg cgcaatgccc gtttttattg
    31681 ccttattcca cgcctgacga atcaatttga tcacacctga gaaaatgcat agccagaagt
    31741 taaggccgtt atattgcgat tgggagcgtt ttaaacggta tcaatttaat taattctgat
    31801 gtggtgattt gcaggcaaaa aaaacccgcc ggagaagcgg gaaatagaga catatcgttg
    31861 tagttattaa cagatcactt tgaggtcgag ggcggctaac cctcgctaat attatcccgc
    31921 tattaccttg cgggtgcaaa taatctttta ttgctttgat gatttaataa atagcacttt
    31981 ttgctaacaa aaactgctgt caatatatgg ccaaaaatgt caacagaaaa aataaggttt
    32041 ataatattaa gtatcacatg ggtaatgggt tcagggaatc ggaatccacc gcctcacgga
    32101 aattcaaaat ttgcttcttc tttcctccgt gggcggcttc tttttcgtat cgaggtattt
    32161 atgaaagcaa taacattaga acagttcgca gcaatggcga atgtatcgac cagaacgctg
    32221 aggcgctggc taaaaaacca tcaaccggag tccctttgcc atttacccca cgcggtactc
    32281 gccaattatt ctgggagcac gaatgcaaag aatggttgta cagaacagaa cgataataat
    32341 cacgcatcac attttgaggt aaacatcatg accaatatcg aaagattaga acgctgtgcc
    32401 gatattatgc ggcgcagatg gatttatgac cctgaagcgg gtttattgtt tagccgtgaa
    32461 acaaaagacg ttatcaaagg cagcatgacc aataaagggc atttggttgt aacggtacat
    32521 gaaggtaaat tcaaagtgac actcacctat cagaaagcct gctatgtata cacatacggc
    32581 gcttatgacg agacattata tgaagtgcac cacgttaatt tgaacaaaca ggataaccgc
    32641 atcaagaata ttcgcctgat gccgaaagaa aagcaccgcc ggatacataa aaacgtccgc
    32701 aaacttattc ggctgggcca cacacaatgg cttcaaccgc aattaattga acaatcacta
    32761 taaacacagg agtaaatacc atgcattcat tagacagtta ttttcagcgc acgacagccc
    32821 ctaaatcagc cgcccaggaa cgccgtgagg aattccatga gaaagtgatg cgttctgctg
    32881 attatattgc ggacaagttc gtggaaacgg ttcgtccact tgttgatgaa gtagctgata
    32941 aacttcagtc tgaaatgccg gaagatatgg aaggcactgc aaagcggcga ctcatttgcg
    33001 aattaagtcg tcgttttggc gtgtcaataa gcgcttttaa ataatcgaaa aaagggggga
    33061 aagataatgg acgcatttga ggtactggca aataaaggcg ctgaactggt agcacagcgt
    33121 gataaaaccg ccaacgaagg ggaacgcgca ctgctaaata aacagattaa agcgatccgt
    33181 atggcccagt ttaaattaat cagtaatgaa gttatcgaaa caattaaacc gcagatccca
    33241 caaatcgttg ctgacgctat taaagccgcc ggactggaga aacgcattgc cgggctaaaa
    33301 acgaacgcag agaaaatgga gatgcacaaa cagagcggtg ctccgaatcc cgatgagtac
    33361 ttctcaccgg atgttgagtt tatggcgcag gttgaggaac gtctgggtag ctgtctcacc
    33421 gaggaacagc gtcgctattt tgatggagtg gatagttcgg caggtattga cctgaactcg
    33481 tatttcggtc gtgaaattga gcattttgat gccgccccgt ctatgccgga gccagaacca
    33541 gaaatggcca ccgatcctga agctgagcaa cggaaacgcg tattcaatgc gatagggatt
    33601 cggtactgat cggataaaag aaagccggga attaccccgg ctgacttaac aatgtgacca
    33661 atgaacaaat cggacaagac aaacaggata agcagtctcg aaacaatgtt cgggaactat
    33721 attagcacaa gtccggagaa aatcatcatg ttggcactac gcatcatgca gggcatcgct
    33781 aagaccctcg cagaacatgt cttagacctg aaacattcgc cactgagcaa acaggccatg
    33841 aagcgccaaa cgcttcgtct gtgggctgaa tattcgcttg gaactatcaa taaaatcatc
    33901 gacatgaaat cggggccgag caaccagagc gcggaggaaa tggagttcat caggcgttta
    33961 attctcattc gcagagacat tcatagccag ttacacagcg tgggaatcga tattaatgat
    34021 gggacgggcg attaattttc ggacaagtac tatgcgcgcg cgaggagctg aacatgaagc
    34081 aagatattgc agacaggctg gagatactcg aaggccagcg ggcagaagcg aaacaactac
    34141 gtaagcaggc cagaagagcg cacaggaaca acgaagcaga gttactaacc aaatacatca
    34201 gtttcaccaa ttactgcatc tacgagtgtt acaaggaaga tgcagaggac tggctggatt
    34261 cattgccaga acagtactga gcagaataac accacgtcat gttgttctga ggtgagcata
    34321 tcacctctgt tcaggtggcc aaatttagtg tgccacttca gcatcaccgt tgaaatcaca
    34381 ataacgattt ttttgtaaat atgctctcac atatgtagga atagaccagg ttggcattga
    34441 aacacatcta ccatgaccat aataaagtaa ttttttgata tgtttatggt tttatattaa
    34501 ttaaaatata tctaaaattg atagttttaa aattgcaaaa tccagattgt gtttgtaatg
    34561 tttaatctga ttgtattgag tggcgtttat tatgaaaaat aaatcaggta attcttgtta
    34621 tagagaagca gtcgatattc atagctcact tgagctttca tttatattgc ataaatatca
    34681 tttgcgtgta gatgagaaga aatctcgaaa tgtattcagc gccatacgtg atgagctcat
    34741 ccttgatggg gattcacgac aaaacttagc tactttctgt caaacatggc ttgatgatga
    34801 aatccgtgag ttgcttaatt tagctattga taaaaacatg atttataaag atgaatatcc
    34861 acaaatggcg gaacctgaag gtcgctgtgt gcgtatgctt tccgattcgt ggagtttccc
    34921 cgattcgcga cacaccttag gttgctcaac cattggttca tctgaagctg ctatgctcgg
    34981 ccgcttggcg ttaaaatggc agtggtgtaa aaaacgcgaa gtacaaggta aatcaaccga
    35041 acctgatttg cgatcctgtg caaatatgct ggcataaatt ctgccgttac tgtgatgtga
    35101 aattgtgtga aattccactt gatgatcatc acctgataat gaatgctaaa gaggttttga
    35161 agcatgtcga tgaaaacact attggtgtcg ttacgacttt aggtattact tatttttgta
    35221 aagcagattg cgttagtgtt tttaagataa gtgttaaagt taatattagt ttttatattt
    35281 acatcgtaat tatatgtttc tattttaaaa atgttcgaga gtataaaaat gtctaatgct
    35341 aatatcgcac ccaaaagtaa gaaaatgaca gtggggacac tcgctatcat gaatataact
    35401 gctgtagtaa gtctacgatt tataccttct gaagcagaat acggcttagg tgccattttt
    35461 tactatacct ttgctgcaat tatgttttta gtacctgttg cctttgttgc ggctgagcta
    35521 gctacaacat atccacaaaa agggggggca tttcgatgga taggagaagc atttggggct
    35581 cgctggggtt ttgtaggaat gcttatgacc tggctacaag tcatccccta ctttccaaca
    35641 gtactgacat ttggtgcagt ctcggttgca tttattgatg ttgatatggg ggtggcagaa
    35701 tcaattgcca gtaataaatg gtatatttta tttttcgtac tatttgttta ctggggagct
    35761 atttgtgttg cattacgtgg cgttggtatt ttctcgcagg tatctaagtg gtgcggtatt
    35821 atagggacaa ttattccagc tattatcctt gttattcttg gtttctccta tctgtggttc
    35881 agcggtaaac cagcggtgat cccattaggt tggggtgatc ttttccctaa tatagctaac
    35941 ttccagaata ttgttcttgc cgccagcata tttcttgctt atgcagggat ggaaatgaat
    36001 gcggtccatg ttaacgattt agataatcca actaaaaaat acccaattgc aattaccatc
    36061 gcttctatag gtactattgc aatattcctg ctcagtactt taggggtcgc ctttattatt
    36121 cctacagata aaattagtct aactcaaagt ttgttggttg cctacgatat gctgttcgaa
    36181 tgggctggag tgccatggct tggctctgtg atggcattta tgctagcaat tggcgtactg
    36241 ggtggggtgg ttacttggat tgcagggcct aatacaggtg tattagcgat cgcgaaagct
    36301 ggttatttac ctaagttttt ccaaaaaacc aatcgtcatg gaatggggca ccatctaatg
    36361 tttgtacaag gtataattgt ctcagttctt tctgttactt ttgtgataat gccttcagtt
    36421 caagcagctt ttcagatttt atcacagtta acagttatgc tctatttagt tatgtatatg
    36481 ctcatgttcg ctagtgctat ctatctacga cattctcaac ctgatacaaa acgcccatat
    36541 cgagcccctg ctttattttt ctgggcaaca cttggattct tagggtcttt tctggctttt
    36601 gtattaagtt ttattccgcc cgagcaaatc ccaactggta gtccaaccag ctatgttctt
    36661 tatttgttag tattagttgc cctattttta ttaattccaa tattaattta cgccttccga
    36721 aaatctgact ggaaagatcc aaactctgat ttcgaaccat tttcttggga aaaaagaact
    36781 agtaattaaa ttttccataa gctacaaaag atgaggttaa atatgtttag tgctgaatct
    36841 ccaattggtg taatagactc aggtgtaggt ggattgacat cggtaaagga aataattaat
    36901 ttgcttcctg gtgaagatat tatctattgt ggagataata tgaatgcacc ttatggcaac
    36961 cgttctgccg atgatatcat cagtttgact aaaaaaatgc tgactttttt acagtcaagg
    37021 aatgtcaaac tagttgctgt cgcatgtaat actatatcct caactttgga atccgaagaa
    37081 tacagcggat atgctaagag ctttccgttt cctatcttat ccatcattga acctgcagta
    37141 gaagatgtga ttcgccagca atataaaaat gttggtatta tcgctaccga gtttactatt
    37201 aaaacggggt gtcacaaaga gctaataaaa aaattgaatt caacaataaa tgtatttgga
    37261 gaaccaagca aaaatcttgc aatgttaatt gaggaaggta atcttaatgc tcctgcaatc
    37321 ttgaatgaca tcaaaaaaca tgttaaccat ttaatttccc tccatccagt aaatgaaatt
    37381 attctcggct gtactcacta tccaattgtt cagaattttt ttgaagcagt tgcaccggat
    37441 ataaaattta ttaatcctgc acatgatcag gctatctcta ttaaaaatca cttgggacag
    37501 ctaaatttat taaactcctc aaatatagga actttgcaaa taaatactag tggaagtatg
    37561 gagatctaca aaacggtact aggggaattg agcattacga aacctcatac attcagtatc
    37621 aggcagtttt aaatttatcc ggtgcaccgt aaaatcccgt ccttctgaac tgcaccccaa
    37681 aagttggaca ccaactgagt aaggtgcatt ttatggacaa acccagttac atactgggta
    37741 gtgcatccaa ttagtagaac atgtgttttt cgataaacgc tccgatcact ttttcgtgga
    37801 tctcaactga gcgtgagaag cagattgttt tacgacccaa ccgcttaatg cgggtgcgta
    37861 gcgtcagatt attacgctca atgcgttggg tgaatatttt gccggtcaga tgcttattct
    37921 tcggcacctc ccggccatag ctgccccagt catcgctggt cagcatgccg atgttgaagg
    37981 gtgtaagcag tgccagtagc tcccggcacg tttgatcggt tcggggacca aaagtgtagg
    38041 ccagtacacc gcctgttttg gtgttgtacg cgtacccgag ccaatgttgc cgggctttac
    38101 tgccaacgta gctccattgc tcatcaagct cgcagataaa cgccacatca gcatgggcaa
    38161 caggcgaaga agttattcgc tttggcgcga gttttttaaa gtccggatga cggtgttact
    38221 accaattttc agtgtcctgg cggtatcgcg aaccccggca ccattaaagg ccatttcagt
    38281 tatcagctct ttcatacccg gcttacgtgc ttgataagta taagtgaact gaaacacacg
    38341 gtggcagtca cggcagcgaa gtctgtcacg gcctttaggg ttctgaccat ggcggtaaac
    38401 ctgagctgac tgacaacggg gacaatgaat attaacgctg gtcatgagat aacctcaaaa
    38461 gcccgtatta tacatcagat tcaactaatt agaggcatca cccatccagc attataaaac
    38521 atactaacaa taactaaatt ataataatca caacttacaa tcaaataata aatgcataag
    38581 attaacaatg attttttcgg gttatattta tctaaaaaag cagaatatta tgtaacttat
    38641 tattttctaa gcgaaaattt attgtgcggt cagactaaca aacttgcgta ccacccgaag
    38701 aacatgcgaa gatttaagta ttctttcttt tttggttttt atttttcatt ttctcttttc
    38761 ttctctttcc ggttctgttc tggttttggt tttttccttt ttatttttat tctttcttca
    38821 gggttatgct aaaacaccaa aacatttttc accacaaaag catccaatca tcttgccaga
    38881 ccaatcacct taaccggata cactgccgga tataatccca ctaacaccca gacaccaagc
    38941 accatacctg ataactaccg tattcaggaa ggaaatgcga gtgatttcac cgtcaccggc
    39001 acaaccgatc cgccaaaaag aggcgtacca atgtcgatat agtcccccgc gcggacaatc
    39061 acttcgtcaa tgactgccaa cgggagttgt tgtagctgtg ggcgcaacaa catgcccaga
    39121 gctttaccaa agtcctgccc ggccaccacc agcaggggat gcggattcgg aaaacgcgcg
    39181 acgaaatcga ccagcgcgtt gatgaccgtc agaaccgcgg cgtaacgcac aggcagcgag
    39241 gcgggaagcg ccagcacgta cacgtcagtt ttgggatcaa gatccagctg aagcagcgcc
    39301 tgttgccagg cactcaccag atccgtttca tcaatcggga tcgccaccgg caaattgcgc
    39361 agcggcagtt gtacgccctc cagccagatt gtgctgccag agagcgaaag ggtatgtgca
    39421 cccgcgccaa tcaccgtgga gcgtacggtt tgcgccggaa actgcacatt catctcacgc
    39481 aggcgcggat ggtcatgcag cgccgttgcc agaagtgggc caatatcggc aaaacagaac
    39541 gggttggcgg gctggtggcg ataacattcg cccacgcctc cagaaagcgt aatgatttcg
    39601 ggcgtaacac ctgcgggcag caaaccggtt tgcatcaatg cctgcgcgag cggcgaaagc
    39661 gttccgtcaa tcacttcgac aatcagcgcc gccatccgcc gggtaacctg caccagctgc
    39721 gcgccggtca gcgaacgggc gtcagtgcct gcaccgaagc actcatccac aatcatctgc
    39781 cccggtttat gagcgtaaac cacgcgcccc tggctgtcgg tttccagcag gcgaccaccg
    39841 acgttgagac aggcagtgcc gctgattttt ccggcatcga acagggcgta gttcgcggtg
    39901 ccaccgccga tatcgatatt cagtacccga cacagccgtt gttcagaaag ggtttgcgcc
    39961 ccggctccgt gaccggcgat cacggattcg aggtgcggcc cggcgctggc gacgacaaaa
    40021 tcgcccagcg actgtttttc cttcatcact ttttccatca gctgtgcgac caggctttcg
    40081 gtaaactggc cttccggcag ctgggcaatg atggtttcgc ggatgcgctg gctttcggtt
    40141 ttgtcggctg gcacgctggc gggcacggac gccgttaccg ggatcgattc atcacattcg
    40201 gtgatggtaa aacccagtag atccgccact tcacgggctt ccggggtaat aatgctggcg
    40261 cgcagaacca cggacattgc ctgttcgccg cgcgcgtgtg cttcacgaat atcattcgct
    40321 gtgatgagtt ttttcacctg cttcctcctg tttggtgaga gatgccagat aatccaccag
    40381 ttgctgcacg ctttgcgggt cgtggctgtt tagctcgaaa atcggctctt cgaaacccgt
    40441 ctcaagcaat aactttcgcg ttgcggcgac gtcagcatct ggcatgtccg ttttactgat
    40501 gacggcgatt tgccgtttgc tgacgccaat atccagcaac ccggcaggca accgactttc
    40561 cgggtcattt gcgccatgga catagatcag catgtctaca tcctgtagcg tggtaattaa
    40621 ggcgtgatac cagcggggat ggctaaaata ttcacccggc gtatcaatat cgcccttatc
    40681 attaaattcc acggccagtg tttttctggc gagggtataa ttcccctgca acgcattaaa
    40741 taacgttgtt tttcctgccc cgacgctacc cacaaacgca attcgtttca tgaatgcctc
    40801 gtattaactt ttggtcattt cacaaagcgt ataatttaat aagcgaccta aaccgctgac
    40861 ggtttgtgat aacgcttctt ctactgcgcc caccgagcca taaatcacca atgcaccgct
    40921 gaagcggtcg agaaaaccga tatgcacatc agcggctttc agcgctaaat caccggcgat
    40981 catcgccgtt tcgccgggag ttagcgtcat aatgccaatt gcgcccgcat ccggaacgcc
    41041 gattttcttc gccagttcct caccgggatg cgcgatgaga tgcgccagtg tgacctgttt
    41101 gccgggcaca aattcctgaa tgatgcgttc tttatccatg agtcgccgcc tgtgaaaaac
    41161 tttcgtgtat cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta
    41221 aaaggtgagg tagatcaata agaatggaga gatatttact gttatgaata aactttcata
    41281 tgcgacgcat gaatataaat aaaataatat cgagatattt tatatactga tttttttatt
    41341 attcgcaaac tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat
    41401 attcagggta agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca
    41461 cggccagcgc caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa
    41521 ccggtttcgc cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc
    41581 gcagtaagtt gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag
    41641 aacctttcaa agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat
    41701 cgccgtgcat ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg
    41761 cctgacgcat tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca
    41821 gagcaacgcg cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct
    41881 ccgccagctc ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac
    41941 cactcggcag cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa
    42001 agacattttt caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg
    42061 catcggcttc cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg
    42121 cacgctgcgc ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc
    42181 agtactcttt aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt
    42241 tgatctgcaa gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga
    42301 tcggtttcgc cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt
    42361 ccccttccgg cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca
    42421 taaacaggtt ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag
    42481 caatcggacg cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct
    42541 taacgatcaa gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat
    42601 cctgaacgcc atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa
    42661 tcgcgcgtac cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg
    42721 cgccacggaa gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac
    42781 ggccggtaca gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg
    42841 gttccgggtt cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct
    42901 gggtcagcac tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat
    42961 cgagggtacg tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg
    43021 gctcacggcc ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca
    43081 gccccagcgc taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca
    43141 ccatccgcac gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag
    43201 tgctgatgat tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc
    43261 gcagtttctg ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc
    43321 cgaaggtcgg ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa
    43381 cttcaatgtc aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca
    43441 tcaccggttt gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag
    43501 agatcaccgc aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt
    43561 cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg
    43621 ttgccagagg cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat
    43681 caagtgcact ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca
    43741 aataaagtgt gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg
    43801 agtctacaga ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat
    43861 cctataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt
    43921 ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac
    43981 ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt
    44041 ggcagacagc tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa
    44101 tccttcgctg ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc
    44161 tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga
    44221 caaactggcg gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac
    44281 agaagttgat gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct
    44341 ggtggacttg tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc
    44401 gacctgggaa ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct
    44461 gacgctgcca ggcgcgggcc tgtgcggaag caggcgtttt tctgatttcg ccgtttgtag
    44521 ggcgtattta tgactggtat caggcacgca agccgatgga cccgtatgtg gtggaagaag
    44581 atccgggcgt taaatcggtg cgcaatatct acgactacta taagcaacac cactatgaaa
    44641 ccattgtgat gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta accggctgcg
    44701 atcgactgac tatcgcaccg aatttgctga aggagctgca ggaaaaagtt tctccagtgg
    44761 tacgtaaatt aatcccgcct tctcagacgt tcccacgccc agcacccatg agcgaagcgg
    44821 agttccgttg ggagcacaat caggatgcga tggcggtaga aaaactgtct gaaggcattc
    44881 gtctgttcgc tgttgatcaa cgcaaactgg aagatcttct tgccgccaaa ctataaacca
    44941 gccacggagt gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc actcagtatg
    45001 gatgcggtac aaaaagccaa ttctggtcat cccggcgcgc cgatgggcat ggctgatatt
    45061 gccgaagtgc tgtggaacga ttttcttaaa cataatccta ccgacccaac ctggtatgat
    45121 cgcgaccgct ttattctttc caacggtcac gcgtcgatgc tgctctacag tttgctacat
    45181 ctgaccggtt acgacctgcc gctggaagaa ctgaagaact tccgtcagtt gcattcgaaa
    45241 accccaggcc acccggagat tggctatacg ccaggcgttg aaaccaccac cggcccgctt
    45301 ggacaaggtt tggcgaacgc cgtcgggctg gcgatagcag agcgtacact ggcggcgcag
    45361 tttaaccagc cagaccatga gatcgtcgat cacttcacct atgtgtttat gggcgacggc
    45421 tgcctgatgg aaggtatttc ccacgaagtc tgttcgctgg caggcacgct gggactaggc
    45481 aagctgattg gtttttacga tcacaacggt atttccatcg acggtgaaac agaaggctgg
    45541 tttaccgacg atacggcaaa acgttttgaa gcctatcact ggcatgtgat ccatgaaatc
    45601 gacggtcacg atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat
    45661 aagccgtcgc tgattatctg ccgtacggtg attggctttg gttcgccgaa taaagcaggt
    45721 aaggaagagg cgcacggcgc accactgggg gaagaagaag tggcgctggc acggcaaaaa
    45781 ctgggctggc accatccgcc atttgagatc cctaaagaga tttatcacgc ctgggatgcc
    45841 cgtgaaaaag gcgaaaaagc gcagcagagc tggaatgaga agtttgccgc ctataaaaag
    45901 gctcatccgc aactggcaga agagtttccc cgacggatga gcggtggttt accgaaggac
    45961 tgggagaaaa cgactcagaa atatatcaat gagttacagg caaatccggc gaaaatcgct
    46021 acccgtaagg cttcgcaaaa tacgcttaac gcttacgggc cgatgctgcc tgagttgctc
    46081 ggcggttcgg cggatctggc tcccagcaac ctgaccatct ggaaaggttc tgtttcgctg
    46141 aaggaagatc cggcgggcaa ctacattcac tacggggtgc gtgaatttgg catgaccgct
    46201 atcgccaacg gcatcgcgca ccacggcggc tttgtgccgt ataccgcgac gttcctgatg
    46261 tttgttgaat acgcccgtaa cgccgcgcgg atggcggcac tgatgaaagc gcggcagatt
    46321 atggtttata cccacgactc aattggcctg ggcgaagatg gtccgacgca ccaggctgtt
    46381 gagcaactgg ccagcctgcg cttaacgcca aatttcagca cctggcgacc gtgcgatcag
    46441 atggaagcgg cggtgggctg gaagctggcg gttgagcgcc acaacggacc gacggcactg
    46501 atcctctcaa ggcagaatct ggcccaggtg gaacgtacgc cggatcaggt taaagagatt
    46561 gctcgtggcg gttatgtgct gaaagacagc ggcggtaagc cagatattat tctgattgcc
    46621 accggttcag agatggaaat taccctgcaa gcggcagaga aattagcagg agaaggtcgc
    46681 aatgtacgcg tagtttccct gccctcgacc gatattttcg acgcccagga tgaggaatat
    46741 cgggagtcgg tgttgccttc taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc
    46801 gattactggt acaagtatgt tggtctgaaa ggggcaattg tcgggatgac gggttacggg
    46861 gaatctgctc cggcggataa gctgttcccg ttctttggct ttaccgccga gaatattgtg
    46921 gcaaaagcgc ataaggtgct gggagtgaaa ggtgcctgat ggtgatcgcc ggatgctgta
    46981 tgttcccctc accctaaccc tctccccaga agggcgaggg gacggtccgt gcgcttttta
    47041 gggccgcatc tgcgattttg tttaacgggt gatccacaac gtgggcctgg cgtctggccc
    47101 atgccagtta tcgcaggtag gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc
    47161 aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac
    47221 cagttcacgc gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg
    47281 gccgttgttg aacggcaaac gcaaccgaac cgggcgcgag gctagcggct ttttacgcga
    47341 cacaatccat gccaaatcaa tggtgttata ggcccctgcc tcacagctaa tcatcatcag
    47401 tgctttatca tcggtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca
    47461 gcgcagacca ttcatccgcc agtttccata atccagcaaa tcgttacgtt cttcgagtga
    47521 gagtggcgtc ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac
    47581 gctgagaggc ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg
    47641 ctgagcatca ataaacaaca acgccgcttt taagccgctc agagaaatgg tctgattgcc
    47701 gtcgcgtaag gtgatcgcct tcccttcctg aattatctgc aaaaacgcgg tgatggttgc
    47761 cgtatcatcg gttactaata accatggtga aatccgccac ttgtcaccac ttaatgccaa
    47821 cggctcacca tctaatagca gccgtggcgc tatctcccct tcagacgcat ccggcgactt
    47881 caacccaccg cgctcaatac gtaaaacggc atcggtatgc gccccagcgc tgcggctcag
    47941 ggtcatcacc agtccattat gatcgcccgt attgcgcgcc acacaaaaat tttgattatt
    48001 acaggtgacc tgccagtcgg aaaacgcccg ttgcgctggt gccgcccaca ccaaagacgt
    48061 cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc
    48121 agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatcg
    48181 gatttgtcgg attgagccaa taataaacaa cgaaatatta ttgctatttt tcagtccatt
    48241 aaatgtgaca tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg
    48301 gttttgatca tctccagcgc ctggctgaac ggcagctcga gcacttcaat atcttcatct
    48361 tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg
    48421 attagctcag tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc
    48481 acttcatagc ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta
    48541 tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta
    48601 gcgacacgga actgacgaat cagaaccacg ctctttttct tcgcgttgta caggaggatc
    48661 gtcgcaccat taccgcgatc gtaaacttca cgtttatggc ggataacttc gccatctttg
    48721 cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct
    48781 ttaatgaggg tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt
    48841 acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc
    48901 cagccagtca ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag
    48961 atccgcacca cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg
    49021 gtactggctt tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga
    49081 ctccagccac ggcatcccgt gtggattgaa gccaaacgcc ataatcaccg catcggctgg
    49141 catgacaaat tcactgccct caatcggtac aggtcgccga cgcccctggg catccggctc
    49201 gccaagacgc gtgcgcagga aacgaattcc gcaaacgtga ccttgctcat tcagctcaag
    49261 cgccaccggc tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacctc
    49321 tttcttcgag cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc
    49381 gccgtggcgc agcgcagtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac
    49441 gacgttaagt ccggcggtat tgataaacgg ctcttccggt agctcttcga gccccatcac
    49501 ctgtttagtg ttggcaatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt
    49561 gggtaaaccc gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg
    49621 ttccaaaagc aaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat
    49681 ccccatcgcg ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga
    49741 aggaatgccg aaagtgagca agccaccgat ttctggatgg cgatcgtaca ccgtcaccgc
    49801 cacgccattg cgggtcagaa catccgcaca ggccagccct gccggacctg ccccgataat
    49861 cgccacccgc ttgtccactt tggtgacatg acttaagtca ggacgccaac ctttcgccaa
    49921 cgcctgatct gaaatatagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg
    49981 aatagtacag gcaccttcac acaaacggtc ttgcggacaa acgcgtccgg taatttccgg
    50041 cagggtgttg gtctggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag
    50101 ttcaatccac tgcggaatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc
    50161 gccgaactta aggcagcgcg aggcttcccg ttgtgcctgg tcggcgcgaa atggcagata
    50221 aatttcatca aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc
    50281 gggcgttgcc tgcatttgtt cgactttact cattaccggc atttcttgtg cagcggtact
    50341 ggcatgccac ggttgatgct cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc
    50401 ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc gggcaattct cgacacaggc
    50461 aggaccgttt tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc
    50521 tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga
    50581 tttacagcca atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc
    50641 gccattaggg cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact
    50701 acgttgctgc tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac
    50761 atgttgctca tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca
    50821 ttgctgactg ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa
    50881 ccttattctt tatatgagtg ttgttattac ccgacttaca ggggaaccgg caatgttcat
    50941 gtgcccagaa taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact
    51001 gactaaattg cgtttcagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt
    51061 gattgttaaa cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct
    51121 gtcgattctt tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga
    51181 ggctatcaat attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca
    51241 aagtggcagt ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcacc
    51301 ggtattaacc aacctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca
    51361 tctgaatgcc aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta
    51421 tcaggccaat attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca
    51481 ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat
    51541 tttcacgctg gtctttttta ctttgcgccg catacgccat caggtggttg ccccgctgaa
    51601 tcagctggtt accgccagtc agcgtattga acacgggcag ttcgactcgc cgccgctgga
    51661 taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaaa tgtcgagcga
    51721 gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca
    51781 cgagaccaag cgtcgtctgg aggtgttgta tcagtgcagt gttcccaggc gctgaacacc
    51841 agccagattg atgtgcattg tttccgccat attttgcaga ttgttcgcga caatgaagca
    51901 gctgaatatc tggagctgaa cgttggggat aactggcgga ttagcgaagg gaagccaaac
    51961 cctgagctgc caatgcagat tttaccggtg acaatgcaag agacggttta cggcgaactg
    52021 cactggcaaa atagtcacgt ttcatcatca gaaccgctgc ttaacagtgt ttcatcgatg
    52081 ctgggacgcg gtttgtactt taatcaggcg cagaagcatt ttcagcagtt attgttgatg
    52141 gaagaacgtg cgaccatcgc ccgcgaattg cacgactcgc tggctcaggt gctttcttac
    52201 ttacgtatcc agttgacgtt actgaagcgt tcgataccgg aagataacgc caccgcacaa
    52261 agtatcatgg ccgatttttc ccaggcgttg aatgatgctt atcggcagtt acgcgagctg
    52321 ttgactactt tccgcctgac gctgcagcag gcggatctcc cctccgcgtt gagggaaatg
    52381 ctggatacgt tacaaaatca aaccagcgct aaactgaccc tcgactgccg tctgccaacc
    52441 ctggcactgg atgcgcaaat gcaggtgcat ttgttgcaaa ttattcgcga agcggtgctg
    52501 aatgcgatga agcacgccaa cgccagcgaa atcgccgtca gttgcgtcac cgcgccggac
    52561 ggcaatcaca cggtttatat ccgtgataac gggattggta tcggtgaacc gaaagaaccc
    52621 gaaggtcatt atggactgaa tatcatgcgc gaacgcgcgg aacggctggg cgggacgctg
    52681 actttttcac aaccttccgg cggcggcacg ttagtgagta ttagctttcg ctccgcagag
    52741 ggtgagggga aggggaacac gccggctgag gtgtaaataa ttaccctcac accggccctc
    52801 tccctttaaa ggcgagaggg aaaaacgtgc ccatattgac ctgtaagcga gtgtgtcagc
    52861 aatctcatga taatttacat taactccttt ttttctccac gattggctcg taccttgccg
    52921 ctacagtgaa gcaagtcaag cctacaacaa tacgcagaaa cacgaggtcc tcttttaatg
    52981 gcgaatttct ttattgatcg ccccattttt gcctgggtgc tggcaatcct gttgtgtctg
    53041 acaggtaccc tggcgatttt ttcattgccc gttgaacaat accccgatct cgcgccaccg
    53101 aatgtgcgag tgaccgctaa ctatcccggc gcatcggccc agacgctgga aaacaccgtg
    53161 acccaggtta tcgagcaaaa tatgaccggc ctcgataatc tgatgtatat gtcatctcag
    53221 agcagtggca ccggtcaggc atctgtcact ttaagtttta aagcaggcac cgatccggac
    53281 gaagccgtgc agcaagtaca aaaccagctg caatcagcca tgcgaaagtt accgcaggcg
    53341 gtgcaaaatc agggcgtgac ggtgcgtaaa accggcgata ccaacattct gaccattgcc
    53401 ttcgtctcta ccgatggctc gatggataaa caggatattg ctgattatgt tgccagtaat
    53461 attcaggacc cgttaagccg cgtgaatggc gtcggggata tcgatgccta tggttcgcaa
    53521 tattccatgc gtatctggct ggacccggcg aaactcaaca gtttccagat gacggctaaa
    53581 gatgtcactg atgccattga gtcacagaac gcgcagattg cggttgggca acttggtggt
    53641 acaccttccg tcgataagca ggcgctcaac gccaccatta acgcccagtc actgctgcaa
    53701 acaccagaac agttccgcga tatcaccttg cgggtgaatc aggacggctc agaggtaagg
    53761 ctgggcgatg tcgccaccgt cgaaatgggg gcggagaaat acgattatct tagccgcttc
    53821 aatggtaagc cagcctccgg gttgggggta aaactggcct ccagcgcaaa cgaaatggcg
    53881 acagcggagc tggtgctcaa tcgtctcgac gagctggcgc agtatttccc gcacggactg
    53941 gaatacaagg tggcgtatga aaccacctcg tttgttaaag cctccattga agacgtggtg
    54001 aaaacgctgc tggaagctat cgctctggtt ttcctcgtta tgtatctgtt cctgcaaaac
    54061 ttccgcgcca cgctgatacc cactatcgcc gtgccggtgg tgttgatggg aaccttctcc
    54121 gtactttacg ccttcggtta cagcgtcaac accttaacca tgttcgcgat ggtgctggcg
    54181 atcggtctgc tggtggatga cgccatcgtg gtggtggaaa acgtcgaacg tattatgagt
    54241 gaggaaggac tcactcctcg cgaagccaca cgtaaatcga tggggcagat ccagggggca
    54301 ctggtcggga ttgcgatggt gctttcggcg gtatttgtac caatggcctt cttcggcggc
    54361 accaccggtg ccatctatcg ccagttctct attaccattg ttgcggcgat ggtgctgtca
    54421 gtactggtag cgatgctcct cactccggct ctgtgtgcca cgctacttaa gccactgaaa
    54481 aaaggtgagc atcacgggca aaaaggtttt tttgcctggt ttaaccagat gtttaaccgc
    54541 aacgccgaac gctacgaaaa aggggtggcg aaaattctcc accgtagcct gcgctggatt
    54601 gtgatttatg tcctgctgct tggcggcatg gtgttcctgt tcctgcgttt gccgacgtcg
    54661 ttcttaccgc tggaagaccg gggcatgttt actacctcgg tacaattgcc cagcggttca
    54721 acccaacaac agaccctgaa agtcgttgag caaatcgaga aatattactt cacccatgaa
    54781 aaagacaaca tcatgtcggt gtttgccacc gttggttctg gccctggggg taacgggcaa
    54841 aacgtggcga gaatgtttat ccgcctgaaa gactggagcg aacgcgacag taagaccggc
    54901 acctcgtttg ccattatcga gcgtgcaacg aaggcgttta accaaattaa agaagctcgc
    54961 gttatcgcca gcagcccacc agcaattagc ggtcttggta gttctgcagg ttttgatatg
    55021 gagttgcagg accacgctgg agcgggtcac gatgcactga tggcagcacg taatcagttg
    55081 ctggcgctgg cggcggaaaa cccggagcta acccgtgtgc gccataacgg cctcgatgac
    55141 agtccgcagt tgcagattga tatcgaccag cgtaaagctc aggcgctggg cgttgatatc
    55201 gacgatatta acgacacact gcaaaccgcc tggggttcga gctatgtgaa tgactttatg
    55261 gatcgcggtc gcgtgaagaa agtctatgtg caggcagctg cgccgtatcg catgctgcca
    55321 gatgacatca atctctggta tgtccgaaat aaagatggcg gcatggtgcc cttctctgct
    55381 ttcgcgacct cacgctggga aacaggctcg ccgcgtctgg aacgctataa cggttattct
    55441 gcggttgaga ttgttgggga agccgcaccg ggggtcagta ccggtacggc gatggatatt
    55501 atggaatcgt tagtgaagca gctgccaaac ggctttggtc tggagtggac ggcgatgtcg
    55561 tatcaggagc ggctttccgg cgcgcaggct ccggcgctgt acgccatttc cttgctggtg
    55621 gtattcctgt gtctggcggc attgtatgaa agctggtcgg taccgttctc ggtaatgctg
    55681 gtcgtgccgc tgggggtaat cggcgcgctg ctggcaacct ggatgcgcgg gctggaaaac
    55741 gacgtttact tccaggtggg cctgttaacg gtcattggtt tatcggcgaa aaacgccatc
    55801 ctgatcgtcg agtttgctaa cgagatgaac caaaaaggcc atgacctgtt tgaagcgacg
    55861 ctccacgcct gccgtcagcg tttacgcccg attctgatga cctcgctggc atttatcttc
    55921 ggcgtattgc caatggcaac cagcacgggt gccggttccg gtggtcagca tgcggtgggt
    55981 actggcgtaa tgggcgggat gatttcagct actattctgg ctatttactt cgtgccgctg
    56041 ttctttgtgc tggtgcgccg ccgcttcccg ctgaagccgc gcccggaata agcaataaaa
    56101 aaggcgacat gccaatgtgt cgcctttttc aactttccga ttaagaacct gctcagcggg
    56161 ttcttgctgt ttatactttg tctcaggaat tacttacgaa gcataacttc gataaagtct
    56221 ttccagttcc ccagttcacg ttcaatcata acaacctctc ttataattat gggtattcta
    56281 cggaaacaat ataccgtggt gaagctaatt tactcgattg ctgcgatgac tacctccggg
    56341 ggacaaacct tatgtaaata ctatggtcct gcggtgataa tttgtatgtg atacacagca
    56401 acatttcgag atattcatac ggcatctaat actgatttaa ttctggttaa aatacagaca
    56461 gataacaaga tgaatattct taatgtttac gttaaaaatg tttaatatta tttaatagtt
    56521 gttaatttga atacttcgat aatgttatat tttctgataa tcatttgcag gcaaaatgtt
    56581 ttcaccctta aatgagtatt tattctcata aatcgaaaaa ggattcatta tggttacact
    56641 ttacggcatc aaaaattgtg acaccattaa aaaggctcgc cgttggctgg aagccaataa
    56701 catcgactat cgttttcatg attaccgcgt cgatgggctg gacagcgaat tattgaacgg
    56761 ttttatcaac gaattaggct gggaagcgtt actcaacacc cgtggtacaa cctggcgtaa
    56821 actggacgaa actacccgca ataaaatcac cgatgcggcc tctgcggcgg cattaatgac
    56881 tgaaatgcct gcaattatca aacgtccatt gctctgcgcg cctggtaagc ctatgctgct
    56941 gggtttcagt gattccagtt atcagcaatt tttccatgag gtgtagtcta tgtcgtgccc
    57001 ggttattgag ctgacacaac agcttattcg ccgcccttcc ctgagtcctg atgacgcagg
    57061 atgtcaggct ttgttgattg aacgtttgca ggcgatcggt tttaccgttg aacgcatgga
    57121 ttttgccgat acgcagaatt tttgggcatg gcgtgggcaa ggtgaaacgt tagcctttgc
    57181 cgggcatacc gacgtggtgc cgcctggcga cgccgatcgt tggatcaatc cgccgtttga
    57241 acccaccatt cgtgacggca tgttattcgg gcgcggtgcg gcagatatga aaggctcgct
    57301 ggcggcgatg gtggtagcag cagaacgttt tgtcgcacaa catcccaacc atacggggcg
    57361 actggcattt ctgatcactt ctgatgaaga agccagtgcc cacaacggta cggtaaaagt
    57421 cgtcgaggcg ttaatggcac gtaatgagcg tctcgattac tgcctggtcg gcgaaccgtc
    57481 gagtatcgaa gtggtaggtg atgtggtgaa aaatggtcgt cgcggatcgt taacctgcaa
    57541 cctcaccatt catggcgttc aggggcatgt tgcctaccca catctggctg acaatccggt
    57601 acatcgcgca gcacctttcc ttaatgaatt agtggctatt gagtgggatc agggcaatga
    57661 attcttcccg gcgaccagta tgcagattgc caatattcag gcgggaacgg gcagtaacaa
    57721 tgttattccg ggtgaactgt ttgtgcagtt taacttccgc ttcagcaccg aactgactga
    57781 tgagatgatc aaagcgcagg tacttgccct gcttgaaaaa catcaactgc gctatacggt
    57841 ggattggtgg ctttccgggc agccattttt gaccgcgcgc ggtaaactgg tggatgcggt
    57901 cgttaacgcg gttgagcact ataatgaaat taaaccgcag ctactgacca caggcggaac
    57961 gtccgacggg cgctttattg ctcgcatggg ggcgcaggtg gtggaactcg ggccggtcaa
    58021 tgccactatt cataaaatta atgaatgtgt gaacgctgcc gacctgcagc tacttgcccg
    58081 tatgtatcaa cgtatcatgg aacagctcgt cgcgtgatga gtggttctgc aagaggaaat
    58141 aagcatggac tggctggcta aatattggtg gattctggtg attgtctttt tggtaggcgt
    58201 cctgctgaac gtgattaaag atctcaagcg cgtcgaccat aagaaatttc tcgccaacaa
    58261 gccggagctt cccccgcatc gtgatttcaa cgataagtgg gacgatgacg acgactggcc
    58321 gaaaaaggat caaccgaaga agtaagctga gtgctcaacc gtaggccgga taaggcgtta
    58381 acgccgcatc cggcaatggt gaacgatccc tgatgcgacg ctcacgcgtc ttatcaggcc
    58441 aggtaatctg tcttttacat catttcaatc acatcatcat cgccaggctt accgccactt
    58501 agcgcttggt cgaaataatg tttcggaatg gtataacgca agtgatccag ggcaaattgc
    58561 atactgcgat tgtcgatcgc atggcctaaa tcctccacga tatccagcgt cacgtcacca
    58621 ccggcactga ttaacgcttc ctgcgccgca actgcatgcg ccagatcgat aaccggatct
    58681 tcaccaccat gaatcaagtg aattgtcgtg gctgtagacg cggtctccgg caggctcgca
    58741 tagcgtccgt taaaggcaat gacgcgcgaa gcaaggcccg gttcggcttt aatgctctcc
    58801 agcgccataa tcgcgccttg tgagaaacca attagcgccg tggcgttagc ccctactccg
    58861 ctctgtttct gccagtagcg taccgtttca ataaacgtcg gcatgatggc atccacacgc
    58921 gcctggcgat tatcttccgt aataccctgc actgaaaacc actgacgccc cgccggatta
    58981 ccgctcggct ccgcgccacc gacactcacc accagcgcat caggaaacag cggtgcaaac
    59041 cagctgccta tttcccccat tgccaccggg ttatccccga caccgtggaa aagcagtaac
    59101 aactgttgtg caggtttatc cgggctttga acaacaaaat ggtcatgttt catggcgaac
    59161 tccttaactg atgtcattaa ttttacgccg ctgactcaca atgaccatgg gagaaaattg
    59221 aaggagtcag tgaaaaaatt gccattgcgt tatgcgatcg cgcagacgct cagtgcgaac
    59281 ctcatttagt gcgaacagcg cctgcgcggc ctcttcccgc tggcgaacca gtaacatctt
    59341 gcggcctgaa agtttaagtg tggtacataa ctgcgcgtca ctggcgtttt tctgtaaacg
    59401 cccacgcagc gccggtaatg ccagttcact ggtttgtagc agacgcaata agcaacctaa
    59461 cgatgttaat agcggacgat gagcgaaagc aaaaccggcc agttcaagcc agtcgtcgtc
    59521 agaaaggatg gcatcgttta gtggatcaac aggaagcatt tcgccattcc actgcgcgag
    59581 agcttgcgca tcgcgacgta aacggtaatg ctcacgttca gccagctgtt tacccgcatc
    59641 actcatcggt aacagcgcca tcgccgtata gcaaccgctg ctggcttccc gatgattacc
    59701 catccgcacc agcacaaaac cgcagcgttg ccagaaacgc cataactccc cagtgtaacc
    59761 aaaactcacc gaaagatagt cgaggtcatg cgtatattgc aaagctccag caataagttg
    59821 tcgccctgtg ccttcccgct gacgagccgg atgaactgct atccggctaa cccgccttcc
    59881 gcgcaatgtc gccgccagtg gattgctgcc gtgcgccgcc agcgactggg ccaccagatt
    59941 accccgcggg cgacgaaaac ctgcccatac cgcctgactg agttcttgag ataatccgcc
    60001 ctcatccacc agccacaacg ccccggcaat ctcgttttcg ccagccgcct gtaaaaaatg
    60061 ttgccctggt gcatccatca tccggcgtaa atccagcggc gaggtccggt agtgcgcacc
    60121 agacaataac tgataaacct ttaacggcgt ttctggctcg cttcgccata acgtttgttc
    60181 aaatgcggaa atgacgatat tgccttgtgg tgtatgggtg aagttttcat cgttaaaaac
    60241 cagtgcctca ctaaccattt tttccagcgg gcatccctgt gcccagcgga tcggctgttg
    60301 cagttcaaaa cggtgtaaat gcggaaagcg agcgcaaaat ttcagcaaaa aaccacgtcc
    60361 ggtaccttcg tagccctgca ccgtagtggt taacaacgtt cgaggaaaac gcgataccag
    60421 ttgatgcaac aacggcgcag gtatggctgc ggcttcgtcg accaccagcc agtcggcttg
    60481 ctcatcgctg gctaacaagg catccggtgc aataaagcga aacttctcgc ccgcaaattg
    60541 tgccagtaca tccgttgccg cttttgcggg cgcggtgaca atcgcactgc ccgcaatacg
    60601 agaaatgagt tgccctgcca acgccgactt accgcgccca cgcgcagccg ttaccgctgc
    60661 cacgcccggc ggcatggtca gtagctgctg taagagttgc tgttgttctg gttgtggtgc
    60721 gccggtcgcg gggtgccagt cagtacgggg agtaaaatgc gccaacgaga acggctggtt
    60781 ttgccgccag aggatagcgt cgttatccgc cgtaagtacg cgtttgagat gctggacaaa
    60841 atgcggcgtc gcaatagggt cagggcaatc actccagcgc agcgagtcgg catcaggttg
    60901 gttttcccac tcttcccata cagggagtaa caaaaccagc cagcttcccg ctttcaacgt
    60961 tccgctaagt gctgcaaagg cagcggcatc aaagccgtgg cgggcgtcga ataccgcatg
    61021 ccggaactcg cgcccaagta aagtttgtag cgccgagggt gaacagtggt tttcagcatc
    61081 tagctgcggc gaaatccaca gccagtcgcc aggtaaggca tcacgcaact taagagcatg
    61141 atcaaaacac caaccctctt ccccgctcaa caccagcaag cggcagatcc cttcacgttt
    61201 catttgcgct gttaatgtgt aaagcgcagt cagttcagcc atccctgccc cggaaattaa
    61261 atgcttttac caaaagtatt gcattgtgcc ggatcgccgc tgtcgaaacc acgtttaaac
    61321 cagctgtagc gttgctgaga agtgccatgt gtgaaactgt ctggtactac tcgcccctga
    61381 ctttgctgtt gtaaacggtc atcgccgatg gcctgcgccg cattcagcgc ctcttccaga
    61441 tcgccagttt ccagaacgcc ttgctgctgc atactatgcc cccagacacc ggcaaaacag
    61501 tcggcctgga gttccatacg cacagataag cggtttactt ccgcctgcgt cgcgttttgt
    61561 tgcagttgac gaactttcgg ctcgatgcct aacagtttct gcacatgatg accgacttca
    61621 tgggcgataa cgtacccctg ggcaaaatcg ccatccgcgc caagtttgtc tttcatgtca
    61681 tcatagaagg agagatcgat ataaactgtg ccatccgccg ggcaatagaa cggccccata
    61741 atggactggc ccgccccgca gccggtgcgc gtcattccac ggtacatgac cagcttcggt
    61801 tgctgatagg tcttacccat cttttcgaac tgttgtcccc aggtgtcttc cgtggttgcc
    61861 agaatcaccg aggtgaattt tgcggcttcg tcttcattag ggctaattga ccgcgttgat
    61921 tgttgttgag aaaccggctg cccggtcatc aacccggtta aatcaacgcc atagtagcct
    61981 gcaaccagca cgactatcag taaaatcagc ccgcctttac cgctcggcag acggaaaccg
    62041 ggaccgccca ttgacggacc gccagagctg ttgcgcctgt cttcaacatt gtcactttca
    62101 cgtcgccctt gccaacgcat agatacctca acaatatatt cattatagaa atgatcgtag
    62161 gtggttcaga ggaagattac cacaggaaag cgggatgaaa tggcggagga atgagcgttc
    62221 aatgcatata ttccgagagc ggttgtcttg ccgtgccagc tgcacggcaa gatgatgaac
    62281 agaaaaatca gtccagctgt acacccaggc ggcgggcgac ggcttcatag gcttcgatca
    62341 ggccaccgag gctctggcgg aaacggtctt tgtccatttt ctccagtgtt tctttgtccc
    62401 acaggcggct gccgtccggg gagaactcat cgcccagtac cacttcacct ttgtacagac
    62461 caaactccag cttgaagtcg accagaatca gaccagcatc atcgaacagt tttttcagca
    62521 cgtcgttcgc tttgtaggtc agctctttca tacgcgccag gttctctttg ctcacccagc
    62581 caaaggtttc gcagtaagat tcgttgacca tcggatcgtg catggcatcg tttttcagga
    62641 acagatcgaa cagcggcggg ttcagctcaa taccttcttc gattccaaga cgtttcacca
    62701 gagagccagc agcacggtta cgcacgacac actcaaccgg caccatatcc agctttttca
    62761 ccagacattc ggtatcggag agcagacgct ccatttgagt cgggataccc gcttcagcca
    62821 gtttgctcat aatgaagtag ttgaacttgt tgttcaccat acctttgcga tcaaactgct
    62881 caatgcgcgc gccatcccct gctgacgtat cattgcggaa ttcgagcacc aacaggtccg
    62941 ggttttccgt gctgtatacg gttttcgctt taccacgata caactcagct tgcttttgca
    63001 tctttatcac tcctgggtgt gaattaacgt tttaaaatct tttgctgtct ggtgtgccgg
    63061 atgttttgtc ggatgcggcg tgaacgcctt atccgaacta cgtccgacaa acaaatttcg
    63121 tgcgaattac cgctaaaatc gcgttttcct gccgacgcac acgtttgcgt atcatatcag
    63181 aaaaaagggc cggatgattc cagccctgta tttttacttg ctaaacgcag cctggaagac
    63241 agctaccagc gcgtcgttct gactctgagt cagagtatga cctttcggat cgatgaactg
    63301 taggctgctg cggttatcta aatcgccaac ctgcagttta tagtcaccgg atgccaggcc
    63361 tggatcgctc gcgcccagtt cctgccagtc gctgtcagac agcggcttat aagttacggc
    63421 catgttgccc tgcgaacggg tgctgtcggt cactttcatg cccacttttt ccagcgccgc
    63481 tggcagacgt tgccaaacca cattgaacgg cccgcgtacg accagcattg gtaaaccggt
    63541 gtcatcagct gcactttgta cgtccatagt ggtggaggca cgattttgcg cagcgttcgc
    63601 ggcgtcagtg gcagatttat ccagaccggc ggaaataacg ttcatcatct ccgtgctgta
    63661 acgctgcatg gaagccgcgt ctgcaaccgg tttgcccgcc tgttccaggt tcagcagttt
    63721 aaccgtaacc gcctgctgat aaccctgcgg cttaacagag atttgataac gaccacgata
    63781 ctgctcgtct tcgtccagac ggttccattg tacccaatcg gtggtcagtg tctgaccagc
    63841 atcatcacgt tgggtgatgg tgtagttttt cgcctgcagc acgctaacca cctgcggcca
    63901 cagagtattg ccacgaccat tttccaccag caatgaagcg gtatcgcccg tgaactgggt
    63961 acgcgcgcca gaaaccagtg ccaacggctg ggctggtgga cgaatgtcca gcgccttacc
    64021 gacagcacca ctaccgttgg tcaccgggat tgcataatca ccggaggtca ccggcaaaat
    64081 cattccagcc ggggcatgaa gctccgcaag cggtgccgct tccaggtagg cttcatcacc
    64141 actgacctga cgcttatagc gtgagtcaga actacaggca gcgagtaata aaacaagcga
    64201 aacacccgca acctttgcca ggcgcgactt ttgaacagag taagccatca aatctcccta
    64261 aactttacag caaaccggca tgcttaagcg ccgctctgac cgtctcacga ccactgtcgg
    64321 tgattggtgt cattggcagg cgcagcgtat cggtcgccac aagacccagt tccttacatg
    64381 cccatttcac cgggattgga ttgggttcga caaatagttt gttgtgtaat ggcatcagac
    64441 gctgattaat aacgcgtgcc tcggcaaaat gcccttctgc tgccagtttg cacatctggg
    64501 ccatatcacg cgctgcgacg ttagccgtaa cggaaataac tccatgaccg cccaattgca
    64561 tgaagtccag cgcgctcgca tcatcgccgc tcagcagaac aaaatcatct gaaaccagct
    64621 ctttgatctg gtttacacgc gttaagttcc ctgttgcctc tttgattccg ataatatttt
    64681 ttactttcgc cagacggccc accgtttccg ggagcagatc gcagccagta cgggacggca
    64741 cattatacag aatttgcggc aggtcagtat gctcagcgat ggctttgaaa tgctgataca
    64801 aaccttcttg cgacggacga ttgtagtaag gggttaccgt caggcagccg acgataccac
    64861 tgtcattgaa gcgctgcgtc aggctaatgg cttccgcagt agcgttagcg ccggtcccgg
    64921 caattaccgg aatgcgccca tcagccagat ccagcgtcat catcaccaca tcagcatgtt
    64981 cgtcatgatt taaggtagcg gactcgccag tggtgccaac agaaacgatc gccgaagtac
    65041 cgctggcgac atgataatca atcagttttt tcaagctagc ccgacagaca ttaccttttt
    65101 catccatcgg agtaacaatc gcgacaatac ttcccgtgaa catgggccat cctctgtgca
    65161 aacaagtgtc tcaatggtac gtttggtatg gcattaaaag caagcagaca gaaccgttct
    65221 gattgttgta tgcatgattt ttttatgctt tccttaagaa caactcaccc cttaaaggaa
    65281 taaccagttt gacactgtca tcgcaacatt atctggtgat cactgcgttg ggtgccgatc
    65341 gccctggaat tgtgaacacc atcacccgtc atgtcagtag ttgcggctgt aatattgaag
    65401 acagtcgcct ggcgatgctg ggagaagagt tcacgtttat catgctgctt tccggttcat
    65461 ggaatgccat cactctgatt gaatcaacgt taccgttgaa aggtgccgaa ctggatcttt
    65521 taatcgtgat gaagcgcacg acggcgcgtc cgcgtccgcc aatgccagca tctgtctggg
    65581 ttcaggtcga tgttgcagac tccccgcatt taattgaacg cttcacagca cttttcgacg
    65641 cgcatcatat gaacattgcg gagctggtgt cgcgcacaca acctgctgaa aatgaacggg
    65701 ctgcgcagtt gcatattcag ataaccgccc acagccccgc atctgcggac gcagcaaata
    65761 ttgagcaagc gttcaaagcc ctatgtacag aactcaatgc acaaggcagt attaacgtcg
    65821 tcaattattc ccaacatgat gaacaggatg gagttaagta atgaatccac tgaaagccgg
    65881 tgatatcgca ccgaaattta gcttgccgga tcaagacgga gaacaagtta atttgaccga
    65941 cttccaggga cagcgtgttc tggtttattt ctacccgaaa gccatgaccc ccggctgtac
    66001 cgtacaggcc tgcggcttac gcgataacat ggatgagttg aaaaaagcgg gcgttgatgt
    66061 gctgggtatc agcaccgata aacccgaaaa actctcccgt tttgcggaaa aagagctgct
    66121 taactttacg ctcctgtctg atgaggacca ccaggtgtgc gaacaattcg gcgtctgggg
    66181 tgaaaagtcc ttcatgggca aaacctacga tggtattcat cgcatcagct tcctgattga
    66241 cgctgatggc aaaatcgaac atgtctttga cgatttcaaa accagcaatc accacgacgt
    66301 tgtgctgaac tggctgaaag aacacgcctg attactttgc tccattccgt gctggctgcg
    66361 cttgcggcca gcatacctca cttctcgtga tcaagatcac attctcgctt tcccctgcga
    66421 cacgggtgtc gaatccattt tttgctgaac gttaatgacc atcatttttg taccgttcag
    66481 aatccagtta atacataact tattgaatag attgagttaa tcagaatggc atcctttata
    66541 caatatgaaa tgcaatgttt catatcattt tcaaggagcc gacatgaacc gctttgtggt
    66601 ggccgaacca ctgtggtgta caggatgtaa tacctgtctc gctgcctgtt cggacgtgca
    66661 taaaacgcaa ggtttacagc aacacccgcg cctggccctg gcgaagacgt caacaatcac
    66721 tgcccctgtc gtgtgtcatc actgtgagga agccccttgc ctgcaggtct gcccggtcaa
    66781 tgccatctct cagagggatg atgcgatcca actcaacgaa agcctctgta ttggctgcaa
    66841 gctttgcgcc gtggtctgcc catttggcgc aatcagcgct tcaggaagcc gtccggtgaa
    66901 tgcccatgcg caatatgttt ttcaggctga aggctcactc aaagacggcg aagaaaacgt
    66961 gccgccccaa catgcgttgc tgcgctggga accgggtgtc cagaccgtcg cggtgaaatg
    67021 cgacctgtgt gatttcttgc cagaaggtcc ggcctgcgtt cgcgcttgcc cgaatcaggc
    67081 gttacggctg atcaccgacg atagcctgca acgtcagatg aaagaaaaac agcgccttgc
    67141 cgcaagctgg tttgccaatg gcggggagtc tcccctttcc ctcactcagg agcaacacta
    67201 atggatgccc tgcaattatt aacctggtcg ctgatcctct atctgtttgc cagtctggct
    67261 tcgctgtttt tactcggtct ggacagactg gctattaagc tttccggcat cacatcgctg
    67321 gtgggcggcg tgattggcat catcagcgga attacgcaat tacatgctgg tgtaacttta
    67381 gtcgcccatt ttgccacccc ttttgacttt gccgatttaa ccctgcgaat ggatagcctc
    67441 tcggcattta tggtgctagt tatctccttg ctggtggtgg tttgttcgct ctattcattg
    67501 acttatatgc gcgaatacga gggcaaaggc gcggcggcga tgggcttctt tatgaatctt
    67561 ttcatcgcat cgatggttgc cctgctggtg atggacaacg ctttttagtt catcgtgctg
    67621 tttgaaatga tgtcgctgtc ttcctggttt ctggtcattg ccaggcagga taaaacgtcg
    67681 atcaacgctg gcatgctcta cttttttatc gcccacgccg gatcggtgct gattatgatc
    67741 gccttcttgc tgatggggcg cgaaagcggc agcctcgatt ttgccagttt cgcacgcttt
    67801 cactttctcc ggggctggcg tcggcggtgt ttctgctggc ctttttcggt tttggcgcga
    67861 aagccgggat gatgccgttg cacagctggt tgccgcgcgc tcaccctgcc gcaccatcgc
    67921 acgcttcggc gttgatgtct ggcgtaatgg tcaaaatcgg tattttcggc atcctgaaag
    67981 tagcgatgga tctgctggcg caaacgggtt tgcctctgtg gtggggcatt ctggtgatgg
    68041 cgatcggcgc aatctccgcg ctcctgggcg tgctatatgc gctggcggaa caggatatca
    68101 aacggctgct ggcctggagt accgtcgaaa acgtcggcat tattttgctg gcagtcggtg
    68161 tggcgatgga cggtctgtca ctgcacgacc cgctgctcac cgtggttgga ctgctcggcg
    68221 cactgtttca tctgctcaac catgcgctgt tcaaagggct gctatttctc ggcgcggacg
    68281 cgattatttc gcgtttgcat acccacgaca tggaaaaaat gggggcactg gcgaaacgga
    68341 tgccgtggac agccgcagca tgcctgattg gttgcctcgc gatatcagcc attcctccgc
    68401 tgaatggttt tatcagcgaa tggtacacct ggcagtcgct gttctcacta agtcgtgtgg
    68461 aagccgtagc gctacaactt gcgggtccta ttgctatggt aatgctggca gtcactggtg
    68521 ggctggcagt aatgtgcttc gtaaaaatgt acggtattac tttctgtggt gcgccgcgca
    68581 gtacacacgc tgaagaggca caggaagtgc caaatacgat gatcgtcgcc atgctactgc
    68641 tcgcggcact ctgcgtatta attgcgctta gtgccagttg gctggcaccg aagataatgc
    68701 atattgccca tgcgtttacc aatacccctc ccgtcactgt cgccagcgga atagcacttg
    68761 tacccggcac gtttcataca cgggtcactc cttcattact gttgctgtta ctactggcga
    68821 tgcctttgct gccaggcctt tactggctgt ggtgtcgttc gcgccgcgca gcgtttcgtc
    68881 gcacaggaga tgcctgggca tgcggctacg gctgggaaaa cgcgatggct ccgtcaggca
    68941 atggcgtgat gcagccgctg cgtgtggtct tctctgcgtt atttcgtcta cgacaacagc
    69001 tcgaccctac gctgaggcta aataaaggtc ttgcgcacgt caccgccagg gctcagagca
    69061 cagaaccctt ctgggatgag cgggtgatcc gccccatcgt gagcgccacc caacggctgg
    69121 tcaaagaaat acagcatctg caaagcggcg actttcgtct ctattgcctg tatgtggtcg
    69181 ccgcactggt tgtgctgcta atcgctattg ccgtctaagg aaatcaccat gagacaaact
    69241 ctttgcgacg gatatctggt catttttgcg ttagcacagg ccgtgattct gctgatgcta
    69301 accccacttt ttacgggtat ttcccggcag atacgcgcgc gtatgcactc ccgccgcggg
    69361 ccggggatct ggcaggatta tcgcgatatc cacaaactgt ttaaacgcca ggaagttgcg
    69421 ccgacatctt caggtctgat gttccgcctg atgccgtggg tattaatcag cagcatgctg
    69481 gtgctggcga tggccttacc actatttatt accgtttccc cttttgcggg cggcggcgat
    69541 ctgatcaccc ttatctatct tcttgccctg tttcgttttt tctttgctct ttccgggctg
    69601 gataccggaa gtccgtttgc gggagtcggt gccagtcgcg agttgacgct cggcattctg
    69661 gtcgaaccaa tgcttattct ctcactgctg gtattggcgc tgatagcagg ttccacgcat
    69721 atcgagatga tcagcaatac gctggctatg ggctggaact cgccgctaac caccgtactg
    69781 gcgttactgg cctgtggttt tgcctgcttc attgagatgg gaaaaattcc ctttgatgtt
    69841 gctgaagcag aacaggaatt acaggaaggc ccgctgaccg aatattccgg tgccgggctg
    69901 gcgctagcga aatgggggct ggggctgaaa caggtcgtga tggcagcact gtttgtggcc
    69961 ctgtttctgc cctttgggcg cgcgcaagaa ctttctctcg cctgcctgct gacttcactt
    70021 gtcgttacgc tgctcaaggt tttgctgatt tttgtactgg cctcaatcgc agaaaacacg
    70081 ctggcacgcg ggcgtttttt actcattcac catgtgacct ggcttggctt cagccttgct
    70141 gcgcttgcat gggtcttttg gttaaccggt ctgtaaggag cactgacgga atatggaaaa
    70201 tcttgctctg acgacgttaa tgctgccttt tatcggcgca ctggtcgttt cgttttcgcc
    70261 acaacgtcgg gccgccgaat ggggggtttt gttcgccgcg ctgaccacgc tgtgcatgtt
    70321 gtcgctgatc tccgcgtttt atcaggccga taaagttgcc gtcacgttga cgttggtcaa
    70381 cgtgggggat gtggcattgt ttggcctggt cattgatcgc gtgagtacgc tgattctgtt
    70441 tgtggtggtg tttctcggtt tgctggtcac gatctactcc acgggttatc tgacggataa
    70501 aaatcgcgaa cacccgcata acggcacgaa tcgttattac gcatttttgc tggtgtttat
    70561 cggcgcgatg gcgggactgg tagtctcctc gacgctgctc ggtcagttgt tgttttttga
    70621 aattacgggc ggctgctcct gggcgttgat cagttattac cagagcgata aagcgcagcg
    70681 ttcagcacta aaagcgttgc ttatcaccca tatcggttcg ttagggttgt atcttgccgc
    70741 cgccacgctg tttttgcaga ccggaacgtt tgtgcttagc gcgatgagcg agttacatgg
    70801 cgacgcacgt tatctggttt atggcggcat cctgtttgcc gcgtggggga aatcggccca
    70861 gctaccgatg caagcgtggc taccggacgc aatggaagcg ccaacaccga tcagcgccta
    70921 tctccacgcc gcatcgatgg tgaaagtggg cgtttacatt tttgcccgcg ccattatcga
    70981 cggcggcaat atcccgcatg tgattggcgg cgttggcatg gtcatgacac tggtcaccat
    71041 tctttatggc tttctgatgt atttgccaca gcaggatatg aagcggttgc tggcctggtc
    71101 gaccatcact caacttggct ggatgttctt cggcttgtcg ctctccatct tcggctcgcg
    71161 gctggcgctg gagggtagca tcgcctacat cgtcaaccac gcgttcgcta aaagcctgtt
    71221 tttccttgta gcaggtgcgc tgagttacag ctgcggcacg cgcttgttgc cgcgtctgcg
    71281 tggcgtattg cacaccctgc cgttgccagg cgtgggtttc tgcgtggcag cgctggcaat
    71341 taccggtgtg ccgccgttca acggcttctt cagtaaattc ccgctgtttg ccgccggttt
    71401 tgcgttgtca gtggagtact ggatcctgct gcccgccatg attctgctga tgattgaatc
    71461 ggtcgccagt ttcgcctggt ttattcgctg gtttggtcgc gtcgtgccgg gcaaaccgag
    71521 cgaggccgtc gccgatgccg caccgctgcc agggtcaatg cgcctggtgt tgattgtact
    71581 gattgtgatg tcgctgattt ccagcgtaat cgccgcgacc tggttgcagt aaggagatga
    71641 tgaatgaccg gttctatgat cgtaaataat ctggcgggac tgatgatgct gacatcgctg
    71701 tttgtgatta gcgtcaaaag ctatcgcctg tcatgcggat tttacgcctg ccagtcgctg
    71761 gtgctggtgt ctattttcgc cactctctcg tgcctgttcg ccgcagagca actgctgatc
    71821 tggtccgcca gcgcctttat caccaaagtg ctgctggtac cgttaatcat gacttacgct
    71881 gcacgaaata ttccccagaa catcccggaa aaagcgttat tcggtccggc aatgatggca
    71941 ctgctcgcgg cgttaattgt cctgctttgc gcatttgtcg ttcagcccgt gaagctaccg
    72001 atggctaccg ggctgaaacc ggcgctggcg gtagcgttag gtcattttct gcttggcctg
    72061 ctgtgcattg tcagccaacg caatatcctg cggcaaattt ttggttactg cctgatggaa
    72121 aacggctccc atctggtgct ggcgcttctt gcctggcgag caccggaact ggtggaaata
    72181 ggtatcgcta ccgacgccat cttcgccgtc attgtgatgg tgttactggc aagaaaaata
    72241 tggcgtaccc acggcacgct ggacgtgaac aacttgaccg cgctgaaggg ataatgagat
    72301 gagttattct gtgatgttcg ctttactcct gctcacgccg ctgctttttt cgctgctctg
    72361 ttttgcctgc cggaaacggg ggctctctgc gacttgtacg gtgactgtat tacatagctt
    72421 agggatcaca ctgctgctga ttctggcact ctgggtggtc caaactgccg ctgatgcagg
    72481 agaaatattc gctgcgggac tgtggcttca tattgatggt ctgggcggtt tgttcctcgc
    72541 cattcttggt gtgattggct ttctcaccgg tgtttactcg attggctaca tgcgtcatga
    72601 agtggcacac ggcgagcttt cacccgttac actgtgcgat tactacggtt tcttccatct
    72661 gtttttgttc accatgctgc tggttgttac cagcaataac ctgattgtga tgtgggcggc
    72721 gatcgaagcc accaccttaa gctcggcgtt tctggtaggc atttacggtc agcgttcatc
    72781 gctggaatca gcatggaagt acatcattat ttgtactgtt ggtgtcgctt ttggtctgtt
    72841 cggtaccgtg ctggtatacg ccaacgccgc cagcgttatg ccgcaggcag aaatggcgat
    72901 atcctggagc gaggttctta agcaatcgtc cttgcttgac ccaacattaa tgctgttggc
    72961 ctttgtgttt gtgctaattg gctttggtac caaaaccggg ctatttccca tgcacgcctg
    73021 gctgccggat gctcacagtg aagctccgag tccggtcagc gccctgctct ccgccgtatt
    73081 gctgaactgc gcgctgttgg tgctgattcg ctattacatc attatttgcc aggccatcgg
    73141 cagcgatttc cccaaccggt tgttgctcat cttcggcatg ttgtcggttg ccgtggcggc
    73201 atttttcatt ctggtacagc gggacattaa gcgtctgctg gcgtactcca gcgtggagaa
    73261 catggggctg gtcgcggtgg cgctaggcat tggcgggccg ctgggaattt ttgccgcgct
    73321 gctgcacacc ttaaaccaca gtctggcaaa aacgctgctg ttctgcggtt ccggcaatgt
    73381 actgctcaag tacggcacgc gcgatctcaa cgtcgtctgt gggatgctca aaatcatgcc
    73441 atttaccgcc gtgctgtttg gcggcggtgc gctggcgctg gcgctggcag ggatgccgcc
    73501 cttcaacatt tttcttagcg aatttatgac cgttaccgca ggactggcac gtaatcacct
    73561 gctgattatc gtcctgctgt tattgctgtt aacgctggtg ctggcgggcc tggtacggat
    73621 ggctgcgcgg gtgttaatgg cgaaaccgcc gcaggccgtt aaccggggtg atctcggctg
    73681 gttgaccacc tcgccaatgg tgattctgct ggtcatgatg ctggcgatgg gaacgcatat
    73741 tccacaacct gtcatcagga tcctggcggg cgcttccact atagtcctct cagggacgca
    73801 cgacctgcct gcacaacgta gcacctggca tgattttttg ccttcaggca ccgcatctgt
    73861 ttcggagaaa cacagtgaac gttaattcat cgtcaaatcg tggcgaagcg attctcgccg
    73921 ccctgaaaac gcagttcccc ggcgcggtgc tggatgaaga gcgacaaacg cctgaacagg
    73981 tcaccattac ggtgaaaatc aatctgctgc ctgacgttgt acattatctt tattatcaac
    74041 atgatggctg gcttccagtc ctgtttggca acgacgagcg gacacttaac ggtcattacg
    74101 cggtttatta tgccctttca atggaagggg ccgaaaaatg ctggatcgtg gtgaaggcac
    74161 tggtcgatgc cgacagtcgg gagtttccgt cagtcacacc gcgcgtccct gccgcggtct
    74221 ggggcgagcg agaaattcgc gatatgtacg ggctgattcc ggttggcctg ccggatcagc
    74281 gtcgcctggt gttgcccgat gactggccgg aagatatgca tccgctgcgc aaagatgcga
    74341 tggattatcg actgcgccct gaaccgacga cagattccga aacgtatgcg tttatcaatg
    74401 agggcaacag cgatgcgcgg gtgatccctg tcggcccgct gcatatcacc tcctatgaac
    74461 cgggtcactt ccgcttgttt gtggatggcg agcaaattgt cgatgctgat taccgcctgt
    74521 tttatgtcca tcgcggcatg gagaaactgg cagaaacgcg gatgggctac aacgaagtga
    74581 ccttcttatc ggaccgcgtg tgtgggattt gcggttttgc ccacagtgtg gcctatacca
    74641 actcggttga aaatgcactg gggattgagg tgccgcaacg agcacatact attcgctcga
    74701 ttctgctgga agtcgagcgg ctacacagtc atttgctcaa ccttggcctc tcctgccatt
    74761 ttgttggttt tgataccggc tttatgcaat ttttccgcgt gcgggaaaag tcgatgacga
    74821 tggcggaatt gctgaccggg tcgagtaaaa cctacggtct gaatctgatt ggtggtgttc
    74881 gccgcgatat tctcaaagag caacgtctgc aaacgctgaa actggtgcgc gagatgcgcg
    74941 ccgacgtgtc ggagctggta gagatgctgc ttgctacgcc gaatatggaa caacgcactc
    75001 agggcattgg cattctcgac cgacaaatcg cccgtgatta tagccctgta gggccgctga
    75061 tccgcgcagt ggttttgccc gtgatttgcg ctttgatcac ccctacgccg actacggcaa
    75121 tattccaaaa acactgttta cctttaccgg cggcgatgtt ttctcccgcg tgatggtccg
    75181 tgtcaaagag acgtttgatt cgctggcaat gctggaattt gccctcgaca acatgccgga
    75241 taccccactg ctgaccgaag gctttagcta taaacctcac gcattcgcgc tgggctttgt
    75301 tgaagcgcca cgcggtgaag acgtgcactg gagcatgctc ggtgataacc aaaaattgtt
    75361 ccgctggcgc tgtcgtgccg ccacctacgc caactggccg gtgttgcgtt acatgctgcg
    75421 cggcaatacc gtttctgacg caccgctgat tatcggtagc cttgatccct gctactcctg
    75481 taccgaccgt gtgacgctgg tagatgtgcg caagcgccag tcaaaaaccg tgctgtataa
    75541 agagatcgaa cgctacggca ttgatcgtaa ccgttcgccg ctgaagtaag gacagaagat
    75601 gctgaagtta ctgaaaacta ttatgcgcgc cggaaccgcg acggtgaaat atcccttcgc
    75661 gccactggag gtcagcccag gctttcgcgg gaaaccggac ctgatgccca gccaatgtat
    75721 tgcctgcggt gcctgcgcct gtgcttgtcc ggcaaatgcg ctgactatcc agaccgacga
    75781 ccagcaaaat tcgcgcacct ggcagctcta tctggggcgt tgtatttact gcggacgttg
    75841 tgaagaagtg tgcccgacca gagccatcca gcttaccaat aactttgaac tgaccgtcac
    75901 ccataaagcc gatctctata cccacgcgac gttccatcta caacgttgca gccgttgcga
    75961 acgcccgttt gccccgcaaa aaaccgtcgc actggctgct gaattgttgg cacagcaaca
    76021 aaatgctcca caaaaccgcg aaatgttatg ggcgcaagcg agcgtctgcc cggagtgcaa
    76081 acaacgcgcg acgctgatca acgacgatac agatgtaccg ctggtggcta aggagcagct
    76141 atgagtccag tgcttacgca atatgtcagc cagcccatca cgctggacga gcaaacgcaa
    76201 aagatgaagc ggcatttgct acaggatatc cgtcgctcgg cttacgttta tcgcatcgat
    76261 tgcggcggct gcaacgcctg tgaaatcgaa atttttgctg ccatcacccc cgtattcgac
    76321 gcagaacgtt ttggcattaa ggttgtttca tcaccgcgtc acgccgatat tttgttattt
    76381 actggcacag tcacccgggc gatgcgtatg cctgcacttc gggcgtatga gtctgccccc
    76441 gatcataaaa tctgtgtttc ctacggcgcg tgcggtgtcg gcggcggtat tttccacgat
    76501 ctctacagcg tctggggcgg cagcgacacc attgtcccca ttgatgtttg gatccccggc
    76561 tgcccgccaa caccggccgc caccattcac ggtttcgccg tggcgctcgg tttgctgcaa
    76621 cagaagattc acgcggtgga ttatcgcgat cccaccgggg tgactatgca accgttgtgg
    76681 ccgcagatcc cgccatcaca gcgtatcgcc attgagcgag aagcgcggcg gctggcgggc
    76741 tatcgtcagg ggcgagaaat ttgcgatcgg ctcctgcgcc atttaagcga cgatcctaca
    76801 ggaaatcggg ttaacacctg gttgcgcgat gccgacgatc tacgtctcaa tagtatcgtt
    76861 cagcaactct ttcgcgtact ccgggggtta catgactgaa gagtgcgggg aaattgtttt
    76921 ctggacgctg cgaaaaaagt ttgtcgccag tagcgacgag atgccggaac acagctctca
    76981 ggtaatgtat tactcgctgg ctatcggtca tcacgttggc gtgattgatt gtctgaatgt
    77041 cgccttccgc tgcccactga cggaatacga agattggctt gcactggtcg aagaggagca
    77101 agcccgacgt aagatgctgg gggtgatgac ttttggtgag attgttattg acgccagcca
    77161 caccgccctg ttgacccggg cattcgcgcc actggcagat gacgcgacgt ctgtgtggca
    77221 agcgcgtagc attcaattca ttcatctgtt ggatgaaatt gtgctggaac cggctatcta
    77281 tctgatggcc agaaaaattg cgtgagaagg atttctcatt aataaggact gttgatggct
    77341 atgtcagacg aagcgatgtt tgccccgccg caaggaataa caattgaagc ggtaaacgga
    77401 atgctcgcgg agcggttagc gcagaaacac ggcaaggcgt ctttattacg cgccttcatc
    77461 ccgctgccgc cgccgttcag cccggtacaa cttattgaac tgcatgttct caaaagcaac
    77521 ttctattacc gctaccatga tgatggcagc gatgtgacgg caacaacaga gtatcagggc
    77581 gagatggtcg attattcgcg ttacgccgtc cttctcggca gtagtggaat ggcggagcta
    77641 cgctttattc gtacccacgg cagccgtttt actccccagg attgcacact gtttaactgg
    77701 caggcacgca ttatcacccc ggttctgcaa tcatggctca atgatgaagc acagcaggtg
    77761 gcgctgcgtt tgctggagaa agatcgcgat catcatcggg tactggtgga tatcactaat
    77821 gcagtgctgt cacatcttga tctcgacgat ctgatcgctg acgtcgctcg tgagatccat
    77881 cattttttcg gtctggcttc agtcagtatg gtactgggcg atcatcgaaa gaacgagaag
    77941 ttcagcctgt ggtgcagcga tctttctgcc tcacattgtg cgtgtctgcc acacaatatg
    78001 cctggcgaca gtgtattgct gacacaaacg ctacaaaccc gacaaccgac cttgacgcac
    78061 cgtgcagatg atctgtttct ctggcaacgc gacccgttat tactcttact tgcatctaac
    78121 ggctgcgaat ctgcgctcct tataccgctt acctttggca accatacacc gggtgcattg
    78181 ttgctggcgc atacctcttc cactctcttt agtgaggaaa actgccagct actacaacac
    78241 atagccgatc gcatcgctat tgccgttggc aatgccgatg cctgccgtag aatgaccgat
    78301 ttgcaggaaa gtttgcagca agaaaaccac cagcttagca agcagctcct ttcgaatctg
    78361 ggcgtcggtg acattatcta tcaaagccag gcaatggaag acctgctcca gcaggtagat
    78421 attgtggcga agagcgacag tacggtgttg atttgcggtg aaaccggaac cggcaaagag
    78481 gtgatcgcca gagcgatcca tcaacttagc ccgcgacacg acaagccgct ggtcaaaatc
    78541 aactgcgctg ccatccccgc cagtcttctg gaaagtgagt tattcggtca tgacaaaggg
    78601 gcgtttactg gtgcgattaa tacccatcgt ggtcgttttg aaattgccga tggcggcacg
    78661 ttgtttctcg atgaaattgg cgatctgccg ttagaacttc agcctaaact gctgcgcgta
    78721 ttgcaggaac gggagattga gcgtctcggc gggagtagaa cgatcccggt aaatgtcaga
    78781 gtcattgccg ccaccaaccg tgatttgtgg caaatggttg aagatcgcca gtttcgcagc
    78841 gatctctttt atcgcctgaa tgtcttccca ctggaattgc ctccgctgcg cgaccgtccg
    78901 gaagatatcc ctcttttagc aaagcatttc acgcaaaaaa tggcgcgcca tatgaatcgt
    78961 tcaattgacg ccatcccgac cgaggcgcta cgccagttga tgtcgtggga ctggccgggc
    79021 aacgtgcgcg agctggaaaa cgtgattgag cgagcggtac tgctgactcg cggtaacagt
    79081 ctgaatttac atcttaatgt ccgacaaagc cgtttactgc cgacgctaaa tgaagattca
    79141 gcgcttcgca gttcaatggc gcagttgctg cacccgacga cgccagagaa tgacgaagaa
    79201 gaacgtcagc gcattgttca ggtattgcga gaaaccaatg gcattgttgc cgggccccgt
    79261 ggcgcggcga cacgattagg gatgaagcgc accacgctgc tgtcacgaat gcagcgtctg
    79321 ggaatctcgg ttcgcgaggt gttgtaatct gcttttgcag gagtatgcat gagaaacaaa
    79381 ctctctttcg acttgcagtt gagcgccaga aaagcggcaa tcgctgaacg gattgccgcc
    79441 cataaaattg cccgcagtaa agtgtcggtc tttttaatgg cgatgtccgc tggcgtgttt
    79501 atggcgatcg gatttacttt ttacctttcc gttatcgccg atgccccgtc ttcataggca
    79561 ttaacccatc tggtgggcgg cctttgcttt acacttggct ttattttgct ggcggtttgc
    79621 ggcaccagcc tgttcacctc gtcggtaatg acggtaatgg caaaaagtcg gggcgttatt
    79681 agttggcgaa cttggctgat taacgcactt ctggtggcct gcggtaatct ggcaggtatt
    79741 gcctgtttca gtttgttaat ctggttttcc gggctggtga tgagtgaaaa cgcgatgtgg
    79801 ggagtcgcga ttttacactg cgccgagggc aaaatgcatc atacatttac tgaatctgtc
    79861 agcctcggca ttatgtgcaa tctgatggtt tgcctggcgc tgtggatgag ttattgcgga
    79921 cgttcgttat gcgacaaaat cgtcgccatg attttgtcca tcaccctgtt tgtcgccagt
    79981 ggctttgagc actgtatcgc caatttgttt gtgattccgt tcgccattgc cattcgccat
    80041 ttcgccccta cctccttctg gcaactggcg cacagtagcg cagacaattt tccggcactg
    80101 acggtcagcc attttattac cgccaatctg ctcccggtga tgctgggtaa tattatcggc
    80161 ggtgcagtgc tggtgagtat ttgttatcgg gctatttatt tacgtcagga atcctgaaaa
    80221 atcagcccgg cgaaacagtt cgtcgggctg aagattattc ttgcgcgatt tgcccatcgg
    80281 gccaggcgtg gatcacggct ttgatcagcg tcgccaatgg aatggcgaag aatacgcccc
    80341 agaatcccca caaaccaccg aagatcacca ccgataaaat aatcaccagc ggatgcaggt
    80401 taaccgcttc ggagaacaac accggtacta gcaggttgcc gtccagcgcc tgaataatca
    80461 gatacaccgc gaagcagctc cagaattcag tacctgcgcc aaactggaat agcgccacgc
    80521 caaccaccgg aatggtcacc acaaatgcgc caatgtacgg aataagaacc gagaagccga
    80581 ccagcactgc cagcagcagc gaatagttca gcccgaagag caagaacccc agccaggtgg
    80641 cgatccccac cacgatcatc tccagcactt tgccgcggat atagttggtg atttgttgat
    80701 tcatctcctt ccacacctgt cctgccagcc cacggttacg cggcagcacc cggcgaacgg
    80761 cgttcagcat ctgctctttg tctttcagca ggaagaagac catcaatggc accagcacca
    80821 gatagacggc tatggtcagc aaaccgacca gcgaggcgag ggaaattttc accaccgaat
    80881 cgcccatggt cagcatccga ctgcgcatat tttcggccat tgcatcaata atgcccgcgt
    80941 ccattaacac cggatagcgg cgcggcaacg tggcggcaaa gtcagaaagc ttattgagca
    81001 tccccggcat atcgcggatt aagtagatgc cctgttgcca ggcgataggc agtaccacga
    81061 acgccatcag tagcaatata ccgacaaaaa ccaccaatac aatcgacgtc gcccagcggc
    81121 gggagcagcc aatagattgc aggcgcacgg ttggccattc cagcaaataa gccagcacaa
    81181 tagccaccag caacggagca agcaggccac taaagaaaaa gataatgcca aatccggcaa
    81241 ctaaaataac cagcaaggca atcgcttccg ggtcgctaaa acggcggcga taccattgca
    81301 tcaacatttc gagcataaag accttccctg aacctcaaga gcgggattgc gatccgcaat
    81361 tgtatcgaaa tgtcacaaaa aagacttcgc tttttatgac ggattcagga aactgaaaag
    81421 tcatttgagt gggctaatct tcgccgttac actcaaaggc ggcgaggtgg gaacgatatt
    81481 tcacagtatc ggtcaaatga ctatcttcag aaatacagga tagaggttat gttcaggcag
    81541 ttgaaaaaaa acctggttgc aaccctcatt gctgctatga ccattggtca ggtagccccg
    81601 gcgtttgccg acagcgcaga caccttgccg gatatgggaa cctccgcagg aagcacgctt
    81661 tccattggtc aggaaatgca gatgggcgac tattatgtcc gccagctacg cggcagcgcg
    81721 ccgttaatta atgacccgct gttaacgcaa tatattaatt cgctggggat gcgtctggtt
    81781 tcgcatgcca attcggttaa gacaccgttt catttttttc tgatcaacaa cgacgaaatt
    81841 aacgcctttg ctttctttgg cggcaacgtg gtgctgcact ctgccctgtt ccgttattcc
    81901 gataacgaaa gtcaactggc ttcagttatg gcgcacgaaa tctcccacgt cacccaacgt
    81961 cacctggcgc gagcgatgga agatcaacaa cgcagcgcgc cgctgacctg ggtcggcgcg
    82021 ttaggttcta ttttactggc gatggccagt ccgcaggcgg ggatggcggc gctgaccggt
    82081 acactggcgg gaacgcgcca ggggatgatc agtttcaccc agcaaaatga acaggaagcg
    82141 gaccgcattg gtattcaggt gctgcaacgc tcgggattcg atccacaggc gatgccgacc
    82201 ttcctggaaa aattgctcga tcaggcgcgt tactcctcgc gcccgccgga aattttattg
    82261 actcacccgt tgccggaaag tcgtctggca gatgcccgca accgtgctaa tcagatgcgc
    82321 ccgatggtgg tgcagtcgtc ggaagatttc tatctggcga aagcgcgcac actggggatg
    82381 tataattccg gacgtaacca gctcaccagt gatttgctgg atgaatgggc gaaaggaaac
    82441 gttcgtcagc aacgagcggc gcaatatggt cgtgctttac aggcgatgga agccaataaa
    82501 tacgacgagg cgcgtaaaac gctgcaaccg ttactggcgg cagaacctgg caacgcatgg
    82561 tatctcgatc tggctaccga tatcgatctt gggcaaaaca aagccaatga ggcaatcaat
    82621 cgcctgaaaa atgcccgcga tttacgcacc aatccggtgt tgcagctcaa cctggcgaac
    82681 gcttatctgc aaggcggtca accacaagaa gcggccaata ttctgaatcg ctacaccttt
    82741 aataataaag atgacagcaa cggctgggat ttgctggcac aggcggaagc cgcgctaaat
    82801 aaccgcgatc aggagctggc tgcgcgagca gaaggttatg cgctcgccgg acgactcgat
    82861 caggccattt cgctgttgag tagcgccagt tcgcaggtga aattaggcag cctgcaacaa
    82921 gcgcgttacg atgcgcgcat cgaccagttg cgccagctgc aggaacgctt taagccttat
    82981 accaagatgt aattcgcatt aaaggaagaa caatgaccaa acaggtaaaa atctaccata
    83041 acccacgctg ttcaaagagc cgggaaacgc tgaacctgct gaaagaaaac ggcgtggagc
    83101 cagaagtggt gctctacctt gagacacccg ccgatgcggc aacgctgcgc gatttgctga
    83161 aaatgctggg gatgaatagc gcccgtgaat tgatgcgcca gaaagaggat ctttataaag
    83221 aactaaacct ggctgacagt tcgcttagcg aagaggcgct gattcaggca atggtggata
    83281 atccgaagct gatggagcgc ccgattatgg tcgcgaatgg caaagcgcgg attggtcgtc
    83341 caccggagca ggtactggaa atcgtgggtt aagatgtgct ccatcgctag ttgaagcaca
    83401 ttgccggatg cgacgcttgc cacgtcttat ccggcctacg aatactatgg attaacttcg
    83461 cgtaggtcag gcgttcgcgc cgcatccgac aataaacacc ttatctacaa cttcagaatt
    83521 tctttcacaa acggaatggt cagcttacgt tgcgcggtaa tcgacgcacg atccaactga
    83581 tccaacgtca taaatagcgt gcgcatttct ctgtcgagcc gcttcagcaa gaaacgcccc
    83641 acatcttccg gcagttcaaa accacgcaaa cgcgcgcgta actgtagcgc ctgcaactta
    83701 tcttcatcag aaagtggctg caatttgtag atctgccccc agtcgagacg cgacgcgaga
    83761 tccggtaatc ccagattcaa ctgccgcggt ggacgatcgc cggtgatcaa caaccgtgtt
    83821 ttgcccgatt ccagaattcg attgtagaga tcgaaaatcg ccatctccca caactcatcg
    83881 cctgcaatac actcaatgtt gtcgatacag accagcgaca aatgctccat accgtcgagc
    83941 acttccggaa caaaccaggt gcgtttatcc agcgggacat agcccaccgc atcgccacgc
    84001 tgcgacaatt ccgcgcaagc cgcgtgcagc agatggctgc gccccgcgcc ttcgcgtgcc
    84061 cagagataga tgtaaccgct atgttcctga cgcagcacgt tttgcagcgc ggccagtaaa
    84121 gaggagttat cccccggcca gaaacttgca aaggtttcgt cgtcaggaag ataaagtggc
    84181 aaagagagct gtgccggtgt gttcagagat acctcaacca ggatttcaca aaatcgcgag
    84241 aagtttacca cagaatccca tgatgtttga accgggcagc aacactgccc ggtcggtaca
    84301 ttatttgtct gttatatccg cgtcttctgc gtccagcacc acttcttcag ggcgcagcac
    84361 actgatcagt ttgaaaatca aacttaagcc gataccgacg atggtcgcca gcgccatacc
    84421 ttttaactcc gccgcgccga tgtttacctt cgcgccactg acgccgatga tcaaaatcac
    84481 ggaagtcagg atcaggttct gtgctttgtt gtaatccact ttcgattcga tcaaaacacg
    84541 aataccggaa gcaccaatga caccataaag cagcagcgaa acgccgccca tcaccggcaa
    84601 tgggatcatc tggatagcgg cagccagttt accgacgcag gaaagcagga tagcgaaaat
    84661 cgccgccccg ccgataaccc aggtactgta aacacgggtg atcgccatca cgccaatgtt
    84721 ttctccgtaa gtggtatttg gcgtagagcc aaagaagccg gagatcacgg tcgacaggcc
    84781 gttagcaaac atcgaacggt gcagacctgg atcgcgcagc agatcttttt tgacgatatt
    84841 agccgttact accaggtgcc ctacgtgttc ggcaataacc actaacgccg ctggcagaat
    84901 agtcagaatg gcaaaccact cgaagcgcgg cgtatagagg gttggcagtg caaaccagtg
    84961 agcattaata atcggcgtgg tatcgacaat tcccattgcg aaagagagcg cgtaccccac
    85021 cagcacgcca attaaaatcg ggataattgc caggaaacca cgaaacagca cggaacctaa
    85081 aaccgtgacc gccagggtgg taatagagat gatgatggtt ttggagtttg gcgtttgccc
    85141 ttcagccggg agtaaacccg ccatgccggc agctacgccc gccagctcca gaccgatgac
    85201 ggcaacgatt gcgcccattg ccgcaggtgg aaacagcacg tccaaccagc cggtccccgc
    85261 tttcttcacg ataaaagaaa ccaggcagaa cagcacgccg cacataataa agccgcccag
    85321 cgcgacttca taccctaacg gcaacagtaa caataccggt gaaataaagg caaagctgga
    85381 accaagataa gccggaattt tccctttaca gatgaagaga tacagcagcg ttccaatacc
    85441 gttaaataac agtacagtcg ccgggttaat atgaaataag acgggcacca ggacggttgc
    85501 accaaacatg gcgaacaaat gttgcaaact aagcgggatt gtctgtaaaa gtggcggtct
    85561 ttcactcacc ccgatagcac ggcgcgtcat agtattatcc tctgtattat gtgttatagg
    85621 cgctttactc aaaaaaaagc cgactcttaa agtcggcttt aattattttt attctttatt
    85681 tcgtaccaaa gattttgtca ccggcatcgc cgaggcccgg aataatgtat ccgtgctcgt
    85741 tcagtccctg gtcaatcgat gcagtataca gttcgacgtc cgggtgcgct ttttccagcg
    85801 cagcgatacc ttctggcgca gcgaccagca ctagaacttt gatgctgctg cagcccgctt
    85861 ttttcagcag gtcgatggtc gcgataacgg aaccaccggt tgccagcatt gggtcaacga
    85921 tcagcgccat acgctcatcg atgttagaaa ccagtttctg gaagtacggt accggctcca
    85981 gcgtttcttc attacggtac ataccgacaa cgctgatgcg cgcgctcgga acgttttcca
    86041 gcacaccgtc catcatacca agacccgcac gcagaattgg cacaacggta attttcttac
    86101 ctttgatctg gtcgatttct accgggccgt tccagccttc gatagttact ttttccgttt
    86161 cgaggtcggc ggtcgcttcg taagtcagca ggctacccac ttcggaagcg agttcgcgaa
    86221 agcgcttggt gctgatatct tgctcacgca tcagtcccag cttgtgtttg acgagtgggt
    86281 gtttgacttc cacgatcttc atactctttc tcctttgagg ggcagccaca aaaaaaatcg
    86341 acggattata cctcctttct tcaaggcggc aatattattt tcgttgactt tagtcaaaat
    86401 gataacagtt tgagataaag ttattttata ttcaaatggt tatgaaagaa gattattcca
    86461 tccgaaaact aacctttgcc ctggcacaag tcttctttcg ccgcgcgccc ggggaaaaga
    86521 cgtgcaaaaa ggttgtgtaa aacagtctcg caaacgtttg ctttccctgt tagaattgcg
    86581 ccgaatttta tttttctacc gcaagtaacg cgtggggacc caagcagtga ccgataaaac
    86641 ctctcttagc tacaaagatg ccggtgttga tattgacgcg ggtaatgctc tggttggaag
    86701 aatcaaaggc gtagtgaaga aaacgcgtcg tccggaagtg atgggcggtc tgggcggctt
    86761 cggtgcgctg tgtgcattgc cgcaaaaata tcgtgaaccc gtgctggttt ccggcactga
    86821 cggcgtaggt accaagctgc gtctggcaat ggacttaaaa cgtcacgaca ccattggtat
    86881 tgatctggtc gccatgtgcg ttaatgacct ggtggtgcaa ggtgcggaac cgctgttttt
    86941 cctcgactat tacgcaaccg gaaaactgga tgttgatacc gcttcagcgg tgatcagcgg
    87001 catcgcggaa ggttgtctgc aatcgggctg ttctctggtg ggtggcgaaa cggcagaaat
    87061 gccggggatg tatcacggtg aagattacga tgtcgcgggt ttctgcgtgg gcgtggtaga
    87121 aaaatcagaa atcatcgacg gctctaaagt cagcgacggc gatgtgctga ttgcactcgg
    87181 ttccagcggt ccgcactcga acggctattc gctggtgcgc aaaattcttg aagtcagcgg
    87241 ttgtgatccg caaaccaccg aacttgatgg taagccatta gccgatcatc tgctggcacc
    87301 gacccgcatt tacgtgaagt cagtgctgga gttgattgaa aaggtcgatg tgcatgccat
    87361 tgcgcacctg accggcggcg gcttctggga aaacattccg cgcgtattgc cagataatac
    87421 ccaggcagtg attgatgaat cttcctggca gtggccggaa gtgttcaact ggctgcaaac
    87481 ggcaggtaac gttgagcgcc atgaaatgta tcgcaccttc aactgcggcg tcgggatgat
    87541 tatcgccctg cctgctccgg aagtggacaa agccctcgcc ctgctaaatg ccaacggtga
    87601 aaacgcgtgg aaaatcggta tcatcaaagc ctctgattcc gaacaacgcg tggttatcga
    87661 ataatgaata ttgtggtgct tatttccggc aacggaagta atttacaggc aattattgac
    87721 gcctgtaaaa ccaacaaaat taaaggcacc gtacgggcag ttttcagcaa taaggccgac
    87781 gcgttcggcc ttgaacgcgc ccgccaggcg ggtattgcaa cgcatacgct catcgccagc
    87841 gcgtttgaca gtcgtgaagc ctctgaccgg gagttgattc atgaaatcga catgtacgca
    87901 cccgatgtgg tcgtgctggc tggttttatg cgcattctca gcccggcgtt tgtctcccac
    87961 tatgccgggc gtttgctgaa cattcaccct tctctgctgc cgaaatatcc cggattacac
    88021 acccatcgtc aggcgctgga aaatggcgat gaagagcacg gtacatcggt gcatttcgtc
    88081 accgatgaac tggacggtgg cccggttatt ttacaggcga aagtcccggt atttgctggt
    88141 gatacggaag atgacatcac cgcccgcgtg caaacccagg aacacgctat ttatccactg
    88201 gtgattagct ggtttgccga tggtcgtctg aaaatgcacg aaaacgccgc gtggctggat
    88261 ggtcaacgtc tgccgccgca gggctacgct gccgacgagt aatgcccccg tagttaaagc
    88321 gccagctctg ccgctggcgt ttttcaattc acctgtaaat cgcaagctcc agcagttttt
    88381 ttcccccttt tctggcatag ttggacatct gccaatattg ctcgccataa tatccaggca
    88441 gtgtcccgtg aataaaacgg agtaaaagtg gtaatgggtc aggaaaagct atacatcgaa
    88501 aaagagctca gttggttatc gttcaatgaa cgcgtgcttc aggaagcggc ggacaaatct
    88561 aacccgctga ttgaaaggat gcgtttcctg gggatctatt ccaataacct tgatgagttc
    88621 tataaagtcc gcttcgctga actgaagcga cgcatcatta ttagcgaaga acaaggctcc
    88681 aacgctcatt cccgccattt actgggcaaa attcagtccc gggtgctgaa agccgatcag
    88741 gaattcgacg gcctctacaa cgagctattg ctggagatgg cgcgcaacca gatcttcctg
    88801 attaatgaac gccagctctc cgtcaatcaa caaaactggc tgcgtcatta ttttaagcag
    88861 tatctgcgtc agcacattac gccgatttta atcaatcctg acactgactt agtgcagttc
    88921 ctgaaagatg attacaccta tctggcggtg gaaattatcc gtagcgatag catccgttac
    88981 gcgctgctgg agatcccatc agataaagtg ccgcgctttg tgaatttacc gccagaagcg
    89041 ccgcgtcgac gcaagccgat gattcttctg gataacattc tgcgttactg ccttgatgat
    89101 attttcaaag gcttctttga ttatgacgcg ctgaatgcct attcaatgaa gatgacccgc
    89161 gatgccgaat acgatttagt gcatgagatg gaagccagcc tgatggagtt gatgtcttcc
    89221 agtctcaagc agcgtttaac tgctgagccg gtgcgttttg tttatcagcg cgatatgccc
    89281 aatgcgctgg ttgaagtgtt acgcgaaaaa ctgactattt cccgctacga ctccatcgtc
    89341 cccggcggtc gttatcataa ttttaaagac tttattaatt tccccaatgt cggcaaagcc
    89401 aatctggtga acaaaccact gccgcgttta cgccatattt ggtttgataa agcccagttc
    89461 cgcaatggtt ttgatgccat tcgcgaacgc gatgtgttgc tctattatcc ttatcacacc
    89521 tttgagcatg tgctggaact gctgcgtcag gcttcgttcg acccgagcgt actggcgatt
    89581 aaaattaaca tttaccgcgt ggcgaaagat tcacgcatca tcgactcgat gatccacgcc
    89641 gcacataacg gtaagaaagt cactgtggtg gttgagttac aggcgcgttt cgacgaagaa
    89701 gccaacattc actgggcgaa gcgcctgacc gaagcaggcg tgcacgttat cttctctgcg
    89761 ccggggctga aaattcacgc caaactgttc ctgatttcac gtaaagaaaa cggtgaagtg
    89821 gtccgttacg cacacatcgg gaccgggaac tttaacgaaa aaaccgcgcg tctttatact
    89881 gactattcgt tgctgaccgc agatgcgcgt atcaccaacg aagtacggcg ggtatttaac
    89941 tttattgaaa acccataccg cccggtgaca tttgattatt taatggtgtc accgcaaaac
    90001 tctcgccgtc tgttatatga aatggtagac cgcgaaatcg ccaacgcgca gcaagggctg
    90061 cccagtggta tcaccctgaa gctaaataac cttgtcgata aaggcctggt tgatcgtctg
    90121 tatgcggcct ccagctccgg cgtaccggtt aatctgctgg ttcgcggaat gtgttcgctg
    90181 atccccaatc tggaaggcat tagcgacaac attcgtgcca tcagtattgt tgaccgttac
    90241 cttgaacatg accgggttta tatttttgaa aatggcggcg ataaaaaggt ctacctttct
    90301 tccgccgact ggatgacgcg caatattgat tatcgtattg aagtggcgac accgctgctc
    90361 gatccgcgcc tgaagcagcg ggtgctggac atcatcgaca tattgttcag cgatacggtc
    90421 aaagcacgtt atatcgataa agaactcagt aatcgctacg ttccccgcgg caatcgccgc
    90481 aaagtacggg cgcagttggc gatttacgac tacatcaaat cactcgaaca acctgaataa
    90541 ccctatgcca atacacgata aatcccctcg tccgcaggag tttgctgcgg tcgatcttgg
    90601 ttcaaacagt tttcacatgg tcatagcccg tgtggtagat ggtgccatgc agattattgg
    90661 ccgcctgaaa cagcgggtgc atctggcgga cggcctgggg ccagataata tgttgagtga
    90721 agaggcaatg acgcgcggtt tgaactgtct gtcgctgttt gccgaacggc tacaagggtt
    90781 ttctcctgcc agcgtctgta tagtcggcac ccatacgctg cgtcaggcgc tgaacgccac
    90841 tgactttctg aaacgcgcgg aaaaggtcat tccctacccg attgaaatta tttccggtaa
    90901 tgaagaagcc cgtctgattt ttatgggcgt ggaacatacc caaccggaaa aaggtcgcaa
    90961 actggttatt gatattggcg gcggatctac ggaactggtg attggtgaaa atttcgaacc
    91021 tattctcgtt gaaagccgcc ggatgggttg tgtcagcttt gcccagcttt attttcctgg
    91081 cggggtcatc aataaagaga attttcagcg cgctcgcatg gcggcagcac aaaaactgga
    91141 aactttaacc tggcaattcc gtattcaggg ctggaacgtt gcaatgggcg cttccggtac
    91201 cataaaagcc gcccatgaag tgttaatgga aatgggcgag aaagacggga taattacccc
    91261 ggaacgtctg gaaaaactgg taaaagaaat tttacgtcac cgtaatttcg catcgctgag
    91321 tttaccgggt ctttccgaag agcggaaaac agtcttcgtt cctggactgg cgattttatg
    91381 tggtgtgttt gatgctttag ccatccgtga actgcgcctt tctgacgggg cgttacgcga
    91441 aggcgtactg tatgaaatgg aaggacgttt ccgtcatcag gatgtgcgta gtcgcaccgc
    91501 cagcagcctc gccaaccagt atcacataga cagcgaacag gcccgacgag tgctggatac
    91561 cactatgcaa atgtacgaac agtggcggga acagcaaccg aagctggcgc atccgcaact
    91621 ggaggcgcta ctgcgatggg ctgccatgct gcatgaggtc gggttgaata tcaaccacag
    91681 cggtttgcat cgccactccg cttatattct gcaaaacagt gacttgccgg gttttaatca
    91741 ggaacagcag ctgatgatgg cgacactggt gcgctatcac cgtaaagcga ttaagctcga
    91801 cgatctgccg cgctttacct tgtttaagaa gaaacagttc ctgccactga tacagctatt
    91861 gcgccttggc gtattactaa acaatcaacg tcaggcaacc accacaccgc caacattgac
    91921 actgattact gatgacagtc actggacact gcgtttcccg catgactggt ttagtcagaa
    91981 tgcgctggta ctgcttgatc tggaaaagga gcaagaatac tgggaaggcg tggctggctg
    92041 gcggttgaaa attgaagaag aaagtacccc agaaatcgcc gcttaatgct ttgcgggccg
    92101 acgagaaatg tcggcccgca ttattcaggc actttcgcga atgggttcga tttcattcag
    92161 cgtatcaatt aacggttgcg gcttaccaat aagataaccc tgcatataat cgatccccaa
    92221 agagagcacc gcctcgcgga tctcttcgtt ttcaacgtac tctgccacta cccgcatttt
    92281 cttcattcgc gccagatggc aaatcgacgc cacaatctga taatccag
//
LOCUS       DAJVPP010000003        72677 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVPP010000003 DAJVPP010000000
VERSION     DAJVPP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.003"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM34_000197"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM34_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1450780.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQM34_000198"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQM34_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1450781.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQM34_000199"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQM34_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1450782.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQM34_000200"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQM34_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1450783.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQM34_000201"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQM34_000201"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1450784.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQM34_000202"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQM34_000202"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1450785.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQM34_000203"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQM34_000203"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1450786.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OQM34_000204"
     CDS             complement(4598..5320)
                     /locus_tag="OQM34_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1450787.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMAGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6317)
                     /gene="fkpA"
                     /locus_tag="OQM34_000205"
     CDS             complement(5487..6317)
                     /gene="fkpA"
                     /locus_tag="OQM34_000205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1450788.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPKADAKPEADAKAADSAKK
                     "
     gene            6538..6756
                     /locus_tag="OQM34_000206"
     CDS             6538..6756
                     /locus_tag="OQM34_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1450789.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6805..7395)
                     /gene="slyD"
                     /locus_tag="OQM34_000207"
     CDS             complement(6805..7395)
                     /gene="slyD"
                     /locus_tag="OQM34_000207"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1450790.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7490..7690)
                     /locus_tag="OQM34_000208"
     CDS             complement(7490..7690)
                     /locus_tag="OQM34_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1450791.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7700..9437)
                     /gene="kefB"
                     /locus_tag="OQM34_000209"
                     /pseudo
     CDS             complement(7700..9437)
                     /gene="kefB"
                     /locus_tag="OQM34_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9437..9988)
                     /gene="kefG"
                     /locus_tag="OQM34_000210"
     CDS             complement(9437..9988)
                     /gene="kefG"
                     /locus_tag="OQM34_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1450792.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10119..12032
                     /locus_tag="OQM34_000211"
     CDS             10119..12032
                     /locus_tag="OQM34_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1450793.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12032..13054
                     /locus_tag="OQM34_000212"
     CDS             12032..13054
                     /locus_tag="OQM34_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1450794.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLANEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13048..13266
                     /locus_tag="OQM34_000213"
     CDS             13048..13266
                     /locus_tag="OQM34_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1450795.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13320..14189
                     /locus_tag="OQM34_000214"
     CDS             13320..14189
                     /locus_tag="OQM34_000214"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1450796.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSRETVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14244..14648)
                     /locus_tag="OQM34_000215"
     CDS             complement(14244..14648)
                     /locus_tag="OQM34_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS1450797.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14950..15582
                     /gene="crp"
                     /locus_tag="OQM34_000216"
     CDS             14950..15582
                     /gene="crp"
                     /locus_tag="OQM34_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1450798.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15633..17723
                     /locus_tag="OQM34_000217"
     CDS             15633..17723
                     /locus_tag="OQM34_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1450799.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLRLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17790..19010)
                     /gene="argD/dapC"
                     /locus_tag="OQM34_000218"
     CDS             complement(17790..19010)
                     /gene="argD/dapC"
                     /locus_tag="OQM34_000218"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1450800.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19096..19659)
                     /gene="pabA"
                     /locus_tag="OQM34_000219"
     CDS             complement(19096..19659)
                     /gene="pabA"
                     /locus_tag="OQM34_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1450801.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19691..20293)
                     /locus_tag="OQM34_000220"
     CDS             complement(19691..20293)
                     /locus_tag="OQM34_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1450802.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20283..20450)
                     /locus_tag="OQM34_000221"
     CDS             complement(20283..20450)
                     /locus_tag="OQM34_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1450803.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20555..21145)
                     /gene="ppiA"
                     /locus_tag="OQM34_000222"
                     /gene_synonym="rotA"
     CDS             complement(20555..21145)
                     /gene="ppiA"
                     /locus_tag="OQM34_000222"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1450804.1"
                     /translation="MFKSTLAAMAAVFALSALSALSALSPAAMAAKGDPHVLLTTSAG
                     NIELELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPP
                     IKNEADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVK
                     GMDVADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21416..22597
                     /gene="tsgA"
                     /locus_tag="OQM34_000223"
     CDS             21416..22597
                     /gene="tsgA"
                     /locus_tag="OQM34_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1450805.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22859..25402
                     /gene="nirB"
                     /locus_tag="OQM34_000224"
     CDS             22859..25402
                     /gene="nirB"
                     /locus_tag="OQM34_000224"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1450806.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25399..25725
                     /gene="nirD"
                     /locus_tag="OQM34_000225"
     CDS             25399..25725
                     /gene="nirD"
                     /locus_tag="OQM34_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1450807.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25851..26657
                     /gene="nirC"
                     /locus_tag="OQM34_000226"
     CDS             25851..26657
                     /gene="nirC"
                     /locus_tag="OQM34_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1450808.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26676..28049
                     /gene="cobA"
                     /locus_tag="OQM34_000227"
     CDS             26676..28049
                     /gene="cobA"
                     /locus_tag="OQM34_000227"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1450809.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28296..28463
                     /locus_tag="OQM34_000228"
     CDS             28296..28463
                     /locus_tag="OQM34_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS1450810.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28758..30095
                     /gene="frlA"
                     /locus_tag="OQM34_000229"
                     /pseudo
     CDS             28758..30095
                     /gene="frlA"
                     /locus_tag="OQM34_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30116..31138
                     /gene="frlB"
                     /locus_tag="OQM34_000230"
     CDS             30116..31138
                     /gene="frlB"
                     /locus_tag="OQM34_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1450811.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31188..32018
                     /gene="frlC"
                     /locus_tag="OQM34_000231"
     CDS             31188..32018
                     /gene="frlC"
                     /locus_tag="OQM34_000231"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCS1450812.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32015..32800
                     /gene="frlD"
                     /locus_tag="OQM34_000232"
     CDS             32015..32800
                     /gene="frlD"
                     /locus_tag="OQM34_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS1450813.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32900..33631
                     /gene="phoB"
                     /locus_tag="OQM34_000233"
     CDS             32900..33631
                     /gene="phoB"
                     /locus_tag="OQM34_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1450814.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33791..34876)
                     /locus_tag="OQM34_000234"
     CDS             complement(33791..34876)
                     /locus_tag="OQM34_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1450815.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34888..36192)
                     /locus_tag="OQM34_000235"
     CDS             complement(34888..36192)
                     /locus_tag="OQM34_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1450816.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36204..36557)
                     /locus_tag="OQM34_000236"
     CDS             complement(36204..36557)
                     /locus_tag="OQM34_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS1450817.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36568..37446)
                     /locus_tag="OQM34_000237"
     CDS             complement(36568..37446)
                     /locus_tag="OQM34_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1450818.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37443..38669)
                     /locus_tag="OQM34_000238"
     CDS             complement(37443..38669)
                     /locus_tag="OQM34_000238"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1450819.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38670..39833)
                     /locus_tag="OQM34_000239"
     CDS             complement(38670..39833)
                     /locus_tag="OQM34_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCS1450820.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39917..40279)
                     /locus_tag="OQM34_000240"
     CDS             complement(39917..40279)
                     /locus_tag="OQM34_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1450821.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40296..41201)
                     /locus_tag="OQM34_000241"
     CDS             complement(40296..41201)
                     /locus_tag="OQM34_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450822.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41471..42475)
                     /gene="trpS"
                     /locus_tag="OQM34_000242"
     CDS             complement(41471..42475)
                     /gene="trpS"
                     /locus_tag="OQM34_000242"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1450823.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42468..43226)
                     /gene="gph"
                     /locus_tag="OQM34_000243"
     CDS             complement(42468..43226)
                     /gene="gph"
                     /locus_tag="OQM34_000243"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1450824.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43219..43896)
                     /gene="rpe"
                     /locus_tag="OQM34_000244"
     CDS             complement(43219..43896)
                     /gene="rpe"
                     /locus_tag="OQM34_000244"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1450825.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43914..44750)
                     /gene="dam"
                     /locus_tag="OQM34_000245"
     CDS             complement(43914..44750)
                     /gene="dam"
                     /locus_tag="OQM34_000245"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1450826.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44857..46143)
                     /gene="damX"
                     /locus_tag="OQM34_000246"
     CDS             complement(44857..46143)
                     /gene="damX"
                     /locus_tag="OQM34_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1450827.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46235..47323)
                     /gene="aroB"
                     /locus_tag="OQM34_000247"
     CDS             complement(46235..47323)
                     /gene="aroB"
                     /locus_tag="OQM34_000247"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1450828.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47380..47901)
                     /gene="aroK"
                     /locus_tag="OQM34_000248"
     CDS             complement(47380..47901)
                     /gene="aroK"
                     /locus_tag="OQM34_000248"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1450829.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48302..49540)
                     /gene="hofQ"
                     /locus_tag="OQM34_000249"
     CDS             complement(48302..49540)
                     /gene="hofQ"
                     /locus_tag="OQM34_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1450830.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49452..49856)
                     /gene="hofP"
                     /locus_tag="OQM34_000250"
     CDS             complement(49452..49856)
                     /gene="hofP"
                     /locus_tag="OQM34_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS1450831.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49846..50286)
                     /gene="hofO"
                     /locus_tag="OQM34_000251"
     CDS             complement(49846..50286)
                     /gene="hofO"
                     /locus_tag="OQM34_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1450832.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50270..50809)
                     /gene="hofN"
                     /locus_tag="OQM34_000252"
     CDS             complement(50270..50809)
                     /gene="hofN"
                     /locus_tag="OQM34_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCS1450833.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50809..51588)
                     /gene="hofM"
                     /locus_tag="OQM34_000253"
     CDS             complement(50809..51588)
                     /gene="hofM"
                     /locus_tag="OQM34_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1450834.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51708..54260
                     /gene="mrcA"
                     /locus_tag="OQM34_000254"
     CDS             51708..54260
                     /gene="mrcA"
                     /locus_tag="OQM34_000254"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1450835.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54425..54985)
                     /gene="nudE"
                     /locus_tag="OQM34_000255"
     CDS             complement(54425..54985)
                     /gene="nudE"
                     /locus_tag="OQM34_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1450836.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55305..57440
                     /gene="igaA"
                     /locus_tag="OQM34_000256"
     CDS             55305..57440
                     /gene="igaA"
                     /locus_tag="OQM34_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1450837.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57505..58173
                     /gene="yrfG"
                     /locus_tag="OQM34_000257"
     CDS             57505..58173
                     /gene="yrfG"
                     /locus_tag="OQM34_000257"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1450838.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58184..58585
                     /gene="hslR"
                     /locus_tag="OQM34_000258"
     CDS             58184..58585
                     /gene="hslR"
                     /locus_tag="OQM34_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1450839.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58610..59479
                     /gene="hslO"
                     /locus_tag="OQM34_000259"
     CDS             58610..59479
                     /gene="hslO"
                     /locus_tag="OQM34_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1450840.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRECCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59623..61347)
                     /locus_tag="OQM34_000260"
     CDS             complement(59623..61347)
                     /locus_tag="OQM34_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1450841.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61726..63348
                     /gene="pckA"
                     /locus_tag="OQM34_000261"
     CDS             61726..63348
                     /gene="pckA"
                     /locus_tag="OQM34_000261"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1450842.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63424..64776)
                     /gene="envZ"
                     /locus_tag="OQM34_000262"
     CDS             complement(63424..64776)
                     /gene="envZ"
                     /locus_tag="OQM34_000262"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1450843.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64773..65492)
                     /gene="ompR"
                     /locus_tag="OQM34_000263"
     CDS             complement(64773..65492)
                     /gene="ompR"
                     /locus_tag="OQM34_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1450844.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65720..66196
                     /gene="greB"
                     /locus_tag="OQM34_000264"
     CDS             65720..66196
                     /gene="greB"
                     /locus_tag="OQM34_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1450845.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66293..68614
                     /locus_tag="OQM34_000265"
     CDS             66293..68614
                     /locus_tag="OQM34_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1450846.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69052..69279
                     /gene="feoA"
                     /locus_tag="OQM34_000266"
     CDS             69052..69279
                     /gene="feoA"
                     /locus_tag="OQM34_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1450847.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69296..71617
                     /gene="feoB"
                     /locus_tag="OQM34_000267"
     CDS             69296..71617
                     /gene="feoB"
                     /locus_tag="OQM34_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1450848.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71617..71853
                     /gene="feoC"
                     /locus_tag="OQM34_000268"
     CDS             71617..71853
                     /gene="feoC"
                     /locus_tag="OQM34_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1450849.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72056..>72677
                     /locus_tag="OQM34_000269"
     CDS             72056..>72677
                     /locus_tag="OQM34_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS1450850.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDR"
BASE COUNT        17499 a        19709 c        18382 g        17087 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca aaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccaaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgccatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcttt gcatcagcct tcggcgctgg tttcacatcc
     5581 agcagctcta cgtcaaacac tagggtagaa ttcggtggga tccccggaac acccgctttg
     5641 ccgtaagcca gttctggtgg aataaccagt ttgatcttac cgcctttctt gatgttcttc
     5701 agaccttctg tccaacccgg gataacaccg tccagacgga aagaaagcgg ttcaccacgg
     5761 gtgtaagagt tgtcgaactc tttaccgtcg atcagcgtac ctttgtagtt cactacaaca
     5821 gtatcgctgt ctttcggtgc ttcgccttta ccggcttcta ctacctgata aaccagacca
     5881 gttgaagagg ttttcacacc tttctctttg gcaaatttct cgcggtactc tttacctttt
     5941 gcttcgttat cagccgcgtc tttttccatc ttcgcctgag cagaagactt cacgcgagct
     6001 tcgaatgctt gcagagtctg ttcgatctct tggtcggaga gtttgctctt atcagcaaat
     6061 gcatcctgaa caccagcgat cagctgatct ttatccagtt tgatgcccag tttttcttgt
     6121 tctttaagag agttttccat gtaacgaccc agcgaagcac ccagtgcata agctgatttc
     6181 tgatcgtcat ttttgaacga tgctttgctg tctgcagttg tagcaggttt tgcagcttca
     6241 gcagcaaaag tgattggtgc atgcagggca acggccattg tggtcgccag tagcgttact
     6301 ttaaacagtg atttcatcca tatctccagg atcggggcat ctcaccccag ggttaactat
     6361 tatcagaagg gtactataaa gcgttgtcga acaaatctac atacagacac gccctattat
     6421 catctatttt cagactcttt ttgtttaaat tagtttcgat gaccgcgaaa tgagtgctgt
     6481 ctcgggcagc aaagttaagt agaatccgcg gcgaccattc gacaaaagag gtgaatcatg
     6541 caggatttat cattggaagc acgcctggca gagctggaga gccgactggc ttttcaggaa
     6601 atcaccattg aagaactgaa cgtcacggtg accgctcatg aaatggagat ggcgaaactg
     6661 cgcgatcatc tgcgtctgct gaccgagaag ttaaaagcca gccagccgtc gaacatcgcg
     6721 tcgcaggctg aagaaacgcc accgccacat tattgaggcg taaaaaaagc ggggattccc
     6781 cgcttttttg tcaccttttc ggtattagtg gcaaccacaa ccgccgttgc ctttaccgcc
     6841 acagcagcct tcgccaccgt gttcatgacc gtgatcgtgg ccatgaccgc cgcagcaacc
     6901 gtcgtggtcg tgatcgtggt gatgatcgtg cgcgccgtga acgtggccat gagccagttc
     6961 ttcttcggtc gcttcgcgaa tcgccacaac ttcaacgttg aatttcaggt tctgaccggc
     7021 cagcatgtgg ttaccatcaa ccactacgtg atcgtcttca accgcagtga tttcaaccgg
     7081 taccggaccc tggtcagttt cagccaggaa acgcatacct acctgcagtt catcaacgcc
     7141 cataaatacg tctttaggaa cacgttgcac taggttttcg tcgtactgac cgtaagcgtc
     7201 gttcgcgcca acagcgacat caaatttgtc gccaacttca tgaccttcca gcgccgtttc
     7261 caggccagag atcagggaac cgtgaccatg caggtagtcc agcggcgcac tcaccggaga
     7321 ctcatcaacc aacacaccgt cttctgtacg tacctgatag gccaggctga ccaccaggtc
     7381 ttttgctact ttcatgatat ctcctgagca tgggaagaat agtggcgcag attgtagcgg
     7441 aattctgcag ccgtgtactc actagcttaa aaaaactcgg cgcatatcgc tagtccggat
     7501 gaaaaatccc gatcacttgc tcatctttgt gaacgtgatc gcgggcttct ttgtctgctt
     7561 ctcgcatctg atgtccgcac ttaacacatt caacaatatc aatattattt tcgcgccaca
     7621 tcgccattga atcctgcgcc tgacaggccg ggcattttgc gcccgctata aaacgttttc
     7681 ggattgccat ccttaccctc tactcaaatt catcccagcc gtccagctgg cgtcgttctt
     7741 gttgcatttc acgctggaaa atctcttcca gttcgcgtcg ggcttccctg gcgcgagaaa
     7801 tttgtacggt atcagcatgc atcgggatta actcccgcag cattcgcata tccaggcggc
     7861 gaaaatgcag ttgcgcccgc tgtgcctgat gcggatgcat gccaagcgtg accagcgtct
     7921 tgcgccccag ctctaacgca ctggagaatg tttcacggga aaactgcgtc acccctgcct
     7981 gtaataactc atgcgcttcc acacgtccgc gcgctcgcgc aagaatatgc aaatgcggaa
     8041 agtgctgctg gcatatttcc accagcttca tggtgtcttc cggctcgtta caggtaatga
     8101 cgatagactc agcggcctct gcacccgcag aacgtaaaag atcgacctgc gtggcgtcgc
     8161 cgtaataaac tttgtagcca tatttacgca tcaggttaac ggcgctgata tcccgctcca
     8221 gcacggtaat gcgcatttta tttgccatca gcaaacgacc aatcacctga ccaaaacgcc
     8281 cgaagcccac gacaatgacc tggggtttat catcgttgac ccacggtttt tcgtcttctt
     8341 cttccggtcc gttaaactgg cgggatagcc atttatccac cagcttcatc agcaacggcg
     8401 ccatctggtc gccctggaat aaacgttgtg aagaagcggt agaaaagagg acaaaggcaa
     8461 actcaccgcc ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat
     8521 acaatcgcgc cagcagatac agcacgagaa ttttcaccgc caccagcaca accacgctta
     8581 tcactaccca caacagatgg gtataaagca ccccgaggtt gagcgacatg ccgacagaga
     8641 taaagaacaa accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc
     8701 gatattcact ttccgccagc agcacgcccg caataaatgt accgagcgcc atcgacagcc
     8761 ccagcgcatc cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca
     8821 cttcccgcac gccagaagct gcaataaagc ggaataccgg acgcagtaaa tagcgcccac
     8881 caatcagcat gccgacaaac tccagcacct tcatgccgat cttcatccag tcgaaatgtt
     8941 cgtctgccga ccccgccaac aacggcacta acgccagtgc tgggatcact gccagatcct
     9001 gaaacagcag aaccgaaaac ccgagctggc cggattcgct gcgattcatc cctttctcac
     9061 gcatcaattg caacgccatt gcagttgaag acatcgcaaa gccaatgcca ccgaccaccg
     9121 ccgcctgcca ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca
     9181 gcacctgtgc cgcgcctacg ccaaaaatcg aacgccgcag ttgccaaagt ttggagggat
     9241 tcaactcaag gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga
     9301 tctcatcgac gtcgctaata aaccccagcc cccacgggcc aattgcaatc cccgccagca
     9361 aatatcccaa cacagcgcca atccccagcc gcgaagagaa acagcactcc tgcgagtaaa
     9421 aaatcggaac cttccatcag cggcctcctg gagacagcgg atttgccaac cagtcaccgt
     9481 aggctctggc gtggctcgcc agctcctttg cgctttgccg tctcgcccag taaataatga
     9541 tgggacttaa ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat
     9601 cgctcatcgg gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg
     9661 taatcacgtt acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac
     9721 cacgacttaa tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa
     9781 gaggatgctg aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat
     9841 caataaaaaa atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg
     9901 tggccggttt aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca
     9961 gcagcaaaac tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag
    10021 tcgctgtttt gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca
    10081 taatgataag ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac
    10141 aaattcgtcg cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc
    10201 agaaagtcgg cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga
    10261 aaaatgaaat cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt
    10321 gggtgaatca ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg
    10381 accgtgaata tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc
    10441 acgccattgc gaccattcat agcaagctgg atgctattga cgcatggagt attcgctccc
    10501 gtgccgccag cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa
    10561 gtgatttctc cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt
    10621 cagacttgct gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc
    10681 tggaaaaatg gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact
    10741 tcctcgatcc gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca
    10801 ccggcaacta cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga
    10861 tgtacgaaag ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg
    10921 ccaaagccac caaagcgaag caggcccaga gccgcattaa gatgctggaa cgtatggagc
    10981 tgattgcccc cgcacacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc
    11041 tgccaaatcc gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc
    11101 tcgactcgat taaattgaat ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg
    11161 gcgcaggtaa atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg
    11221 aaattggtct ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc
    11281 tgcgcgccga cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac
    11341 aaaaactgcg tgactacctc ggcggctttg gtttcctggg cgataaagta accgaagaaa
    11401 cgcgccgctt ctccggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc
    11461 gtccgaatct gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg
    11521 cactcaccga agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc
    11581 atttgctgcg ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt
    11641 tcgacggcga tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc
    11701 agaccgacga agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc
    11761 gtcgggaagc ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg
    11821 aaaaagagat ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca
    11881 gcgaactgta tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca
    11941 gcgccaaatc cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg
    12001 agcagatgct gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa
    12061 tgaatttagc agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca
    12121 aactatgctg ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct
    12181 ggagttgccc gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa
    12241 acataaaccg cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc
    12301 ccacggtctg gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg
    12361 cggatgcagc ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga
    12421 cgccagttgg tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt
    12481 cggctattcg ctcggcggta atatgctggc ctgtttgctg gcaaatgaag gcaatgatct
    12541 cccggttgat gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca
    12601 tatggaaaag ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa
    12661 tgccgcgcgc aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa
    12721 atcggtacgt cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc
    12781 cgacgctatc gactattatc gtcagtgtag cgccatgcca atgctgaacc ggatcgccaa
    12841 accgacgctg attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccaaa
    12901 accggaaagt ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg
    12961 ctttattggc ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg
    13021 gttaacaacg tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa
    13081 acgctggaaa atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat
    13141 gagcgtacac ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg
    13201 gtgctggtat ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc
    13261 gaataacctg caaccgtcgg cggacgagat aatagtgata gctaagtcat ggagttacta
    13321 tgtctgccaa acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca
    13381 ccagcctcgc gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag
    13441 gcgacagttt tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg
    13501 acgccgggcg gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac
    13561 aaaccttcat tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct
    13621 acgacgaagc cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac
    13681 cagaacccac tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc
    13741 ataacgttgc gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt
    13801 ggattcaaaa actgatccgc gacaccagcg agcgcgggca ctcacgagaa acagtgatgg
    13861 actcggtagt gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca
    13921 cccatcttaa cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag
    13981 gtatcccgtc gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg
    14041 atttcccttg gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag
    14101 tggtacctgg cggcaaaatg gggctggcaa tggaattaat tatgctgccg ctggtgcaac
    14161 gattgatgga aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc
    14221 ggcttcacaa cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc
    14281 accgcttttt ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca
    14341 cgcgcaaccg ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc
    14401 gtaaacaggc gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc
    14461 acatcctgac gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc
    14521 gccatcaaca ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa
    14581 atctggtgac cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc
    14641 gcttgcattt ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa
    14701 atcgcgcaac ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg
    14761 aaagctatgc taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca
    14821 aaggacgtca cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat
    14881 atttcggcaa tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga
    14941 taaccgcgca tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct
    15001 cattgccaca ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg
    15061 gaaacgctgt actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt
    15121 aaagaaatga tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt
    15181 gaagagggcc aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa
    15241 atttcgtaca aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg
    15301 tctgcacaga tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc
    15361 ctcgacgtga cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct
    15421 atgactcacc cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc
    15481 ggctgttctc gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc
    15541 tccgcacacg gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg
    15601 ttacctggta gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg
    15661 atatcaacta tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa
    15721 tgcttggcga attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg
    15781 cgggccttga tacgcctcat aaacgttttt tcaaacactt aatcattggt gcgtcgctgt
    15841 ttgccacctg tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt
    15901 tgctgaccgg attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa
    15961 aattgcttcc tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc
    16021 cggtctggga accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact
    16081 ggttttggtt ctggatctgg cgcgaacaac cgctgcgcga gtcactacgt ctgctgtacc
    16141 gtgaactggc agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg
    16201 aaaaagcgct gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta attactcagt
    16261 gctatcagca aatgcatatg ctttccgcgc aaaacaatac cgattacaag cggatgctgc
    16321 gtattttcca ggaggcgctg gatttgcagg aacatatttc ggtcagtttg catcagccgg
    16381 aagaggtgca aaaactggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc
    16441 aaaccgtcgc cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa
    16501 cgcgttttac gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg
    16561 ataatccggt tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca
    16621 cccaaaaacc gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttgc
    16681 ttccggcact gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac
    16741 tcagtgtgat gttaagcgtt gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt
    16801 actggatcct gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga
    16861 ggattgtgaa tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc
    16921 actttaaaat tcccgaaagt tacaccctga cgttgatgct gattaccacc ctcgccagct
    16981 acctgatatt gcgcaaaaac tacggctggg caacggtcgg ttttactatt accgcagtgt
    17041 ataccctgca actgttgtgg ctgaacggtg agcagtacat ccttccacgt cttatcgata
    17101 ccattattgg ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga
    17161 gcgggttatt gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa gcgattcgct
    17221 tgattctgag cgaggacccg caaccaacgc cactggcctg gcagagaatg cgggtaaatc
    17281 aggcgcataa cactctgtat aactcattga atcaggcgat gcaggaaccg gcattcaaca
    17341 gccattatct ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata
    17401 ttaatgccat gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag
    17461 agtatttaca gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg
    17521 aaccgggtag ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag
    17581 gcgcggcagg tacgctggag cagcatttac agcgggttat tggccatctg aacaccatgc
    17641 acaccatttc gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca
    17701 agttgcggga ttcgaaggcg taatacaggc taaaccatcg gataaggcat tcacgccgca
    17761 tccgacatat tttgcctgat acgtcttatt tacgccccaa ccaccttcgc caccgcgtgg
    17821 gcgaaacgtt gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgctaaa
    17881 cgcatcacat ccggtccggc attcagcacc attacgccaa cctctgcacc cgcatacagg
    17941 aaatcacgcg cccgaccttt gtagtgtggt ttcagctccg cgccaattaa cagccccata
    18001 ccgcgaatat cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa
    18061 cgctggcgtt tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc
    18121 gcgcccgcta ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga
    18181 tgaaacgcag aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct
    18241 aacgctttcg cagaagtcag aatatccggc gtcacaccgt agtacctgta agcaaacaaa
    18301 tcgccagtcc gccccatccc gcactgcact tcatcaaaca ccaataatgc ctgatgttga
    18361 tcgcacagct cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg
    18421 ccctggatcg gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca
    18481 tggagatcgt taaagggaac gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa
    18541 tattttggct gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg
    18601 aaggcaataa ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt
    18661 ttaaaggcgg tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac
    18721 gtcgcctcaa tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg
    18781 atatgccaca gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg
    18841 cccaacgccg taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc
    18901 cagattcggc tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg
    18961 atcacttcat cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca
    19021 ccctgttacg cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcactg
    19081 aatggcaatc ggaaatcaga gatgcaggaa attagccagc agttgatgtc cttgttcgct
    19141 aagaatactt tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc
    19201 cataatctcg cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg
    19261 ttccaccacc agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac
    19321 gccctcaccg ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac
    19381 aactttgccg ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg
    19441 caagcgcccg gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca
    19501 ggggccaggt gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa
    19561 cgcatcgttg cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt
    19621 ccaggtaaaa gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa
    19681 ccgcgctatt ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca
    19741 gtgcggtgag atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga
    19801 taccctgcca gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct
    19861 gtgccagacc acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg
    19921 ccagccgctc gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat
    19981 cctgcatcag ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc
    20041 cctgataaat atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct
    20101 ggcgatggat agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg
    20161 cagcacgcag cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt
    20221 tcagccggtt acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga
    20281 atttatcgct catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta
    20341 agagtgacta aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta
    20401 cgctgaagct cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc
    20461 cctgaacgtg tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca
    20521 gaagcgcggg caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga
    20581 taactaccgg ttttgacggc acattctggt acggaccaac atcatgagtc ggcacctggg
    20641 aaatcttatc ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat
    20701 cacgctgacc atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg
    20761 cgctgtcttt gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt
    20821 tatcggcttc atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac
    20881 cgccgccctg aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac
    20941 cgctgttcac ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct
    21001 ccagttcgat gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca
    21061 ttgctgcggg agaaagagca gaaagagcag aaagagcaga aagagcgaaa acagcagcca
    21121 tcgccgccag ggtcgatttg aacatgagat ttccttaaca aacagagcag aaaaaaagca
    21181 agtgaagaga ttctaaatag ccttcaagaa caaggccatc cctttaccta attttacgta
    21241 tctgaaatat atgtaacctg acgatcactt tttattccgt gatcaaaatc acctcttaaa
    21301 atgcaattta gcaaccgatt gcaataaaac atttaaacag atcataaaat cacctaaaat
    21361 cgcccgctcg cggcatatcc cgctatgcca ttttattttt gaacgcaggc caatcatgac
    21421 taacagcaat cgcatcaagc tcacatggat tagctttctc tcctacgcac tgactggtgc
    21481 gttggttatt gtcaccggga tggtgatggg aaatatcgcc gattatttca atctgcctgt
    21541 ttccagtatg agtaacacct tcaccttcct caacgccggc attttaatct ctatcttcct
    21601 caacgcctgg ctgatggaaa tcgtcccgtt gaaaacgcag ttacgttttg gctttctcct
    21661 gatggtgctg gcggttgccg gtttgatgtt cagccacagc ctggcgctgt tctcgacggc
    21721 gatgttcatt ctcggggtgg tcagcggcat tactatgtcg attggtacat tcctgataac
    21781 acaaatgtat gaagggcgtc agcgcggttc ccgcctgtta tttactgact ccttcttcag
    21841 tatggctggg atgattttcc caatgatcgc cgcgtttcta ctggcgcgca gcattgagtg
    21901 gtactgggtt tatgcctgca tcgggctggt gtatgtcgct atttttattc tgaccttcgg
    21961 ctgtgagttc ccggcactgg gcaaacgtgc accaaaaacg gatgctccgg tagagaaaga
    22021 aaagtggggg atcggcgtac tgtttctctc tgttgcggcg ctgtgctaca tcctcggtca
    22081 gttaggtttt atctcctggg tgcctgagta tgccaaaggc ctgggcatga gcctgaacga
    22141 cgcgggcacg ctggtgagta acttctggat gtcatacatg gtcggcatgt gggcgttcag
    22201 ctttattctt cgcttctttg atttgcaacg cattctgacc gtactggctg gtctggctgc
    22261 gattctgatg tacgtcttta acaccggaac accagtacat atggcgtggt caattctcgc
    22321 tctgggcttc ttctccagcg cgatctatac caccatcatc accctgggtt cacagcagac
    22381 caaagtaccg tcgccaaaac tggttaactt tgtcctgacc tgcggaacca tcggtactat
    22441 gttgaccttt gtggttaccg gcccgattgt tgaacatagc ggtccgcagg cggcactgct
    22501 tacagcaaac ggtctgtacg ctgtcgtctt tgtgatgtgc ttcctgttag gtttcgtcag
    22561 ccgtcaccgt cagcataaca ccctgacctc tcattaattg ctaatgccgg acggcactat
    22621 cgtcgtccgg ccttttcctc tcttcccccg ctacgtgcat ctatttctat aaacccgttc
    22681 attttgtcca ttttttgcac aaacatgaaa tgtcagacaa ttccgtgact taagaaaatt
    22741 tatacaaatc agcaatatac cccttaagga gtatataaag gtgaatttga tttacatcaa
    22801 taagcggggt tgctgaatcg ttaaggtagg cggtaataga aaagaaatcg aggcaaaaat
    22861 gagcaaagtc agactcgcaa ttatcggtaa cggtatggtc ggccatcgct ttatcgaaga
    22921 tcttcttgat aaatctgatg cggccaactt tgatattacc gttttctgtg aagaaccgcg
    22981 catcgcttat gaccgcgtac acctctcgtc ttacttctct caccacaccg ccgaagagct
    23041 gtcgctggtg cgcgaaggct tctacgagaa acacggcatc aaagttctgg tcggcgaacg
    23101 cgctatcacc atcaaccgtc aggagaaggt gattcactcc agcgccggac gtaccgtttt
    23161 ttatgacaag ctgatcatgg caaccggttc ctacccgtgg atcccaccaa tcaaaggttc
    23221 tgatactcag gactgctttg tctatcgcac cattgaagac ctcaacgcca ttgaatcctg
    23281 cgcccgtcgc agcaaacgcg gtgccgttgt tggtggcggc ctgttaggtc tggaagccgc
    23341 tggcgcgctg aaaaacttag gtattgaaac ccacgttatc gaatttgccc ctatgctgat
    23401 ggcggaacag cttgatcaga tgggtggcga gcagctgcgt cgcaaaatcg aaagtatggg
    23461 cgtgcgcgtt cacaccagca aaaacaccct tgagattgtg caggaaggtg tcgaagcgcg
    23521 taaaaccatg cgttttgccg acggcagcga actggaagtc gactttatcg tcttctctac
    23581 cggtatccgt ccgcgcgata agctggcaac ccagtgtggt ctggacgttg ctccgcgtgg
    23641 ggggattgtc attaatgatt cctgccagac ttccgatccg gatatctacg ccatcggtga
    23701 atgcgcaagc tggaacaacc gtgtatttgg tctggtagca ccaggctaca aaatggcgca
    23761 ggtcgccgtt gaccatattc tgggtagcga aaacgccttt gaaggtgctg accttagcgc
    23821 caagctgaaa ctgctgggcg tagacgtagg tggtattggt gatgcgcacg gtcgcacgcc
    23881 tggcgcacgt agctatgttt acctcgacga aagtaaagag atctacaaac gcctgattgt
    23941 cagcgaagac aacaaaaccc tgctcggtgc ggtactggtg ggcgatacca gcgactacgg
    24001 caacctgctg caactggtgc tgaacgctat cgagctgccg gaaaacccgg attctctgat
    24061 tctgccggcg cactcgggta gcggtaagcc gtctatcggt gttgataaac tgccggacag
    24121 cgcgcaaatc tgctcctgct tcgacgtcac taaaggtgat ctgattgctg ccatcaacaa
    24181 aggctgccac acagttgcgg cgctgaaagc tgaaaccaaa gcgggtactg gctgcggtgg
    24241 ctgtatcccg ctggtcactc aggtactgaa cgcggaactg gcgaaacagg gcatcgaagt
    24301 taacaacaac ctgtgcgaac actttgctta ttcgcgtcag gaactgttcc atttgatccg
    24361 cgttgaaggc attaaaacct tcgaagaact gctggcgaaa cacggcaaag gctacggttg
    24421 tgaagtttgt aaaccaaccg tcggttcgct gctggcctcc tgctggaacg aatacattct
    24481 gaagccggaa catactccgc tgcaggattc taacgacaac ttcctcgcta acatccagaa
    24541 agacggcacc tactcggtga tcccgcgttc tccgggcggt gaaatcaccc cggaagggct
    24601 gatggcagtg ggtcgtatcg cgcgtgaatt taatctctac accaaaatca ccggctccca
    24661 gcgcctggcg atgtttggcg cacagaaaga cgatctgccg gaaatctggc gtcagctgat
    24721 tgaagctggc ttcgaaaccg gtcatgccta tgcgaaagca ctgcgtatgg cgaaaacctg
    24781 cgtgggtagc acctggtgcc gctacggtgt tggcgatagc gtcggcctcg gcgtggaact
    24841 ggaaaaccgc tacaaaggca tccgtacgcc gcacaaaatg aagttcggtg tctccggctg
    24901 tacccgtgaa tgttcagaag ctcagggtaa agacgtgggt attatcgcca ctgaaaaagg
    24961 ctggaacctg tatgtttgcg gtaacggcgg catgaaaccg cgtcatgcgg atctgctggc
    25021 agcggatatc gatcgcgaaa cgctgatcaa atatctcgac cgcttcatga tgttctacat
    25081 ccgtaccgcc gacaaactga cgcgtaccgc gccgtggtta gaaaacctcg aaggcggcat
    25141 cgattacctg aaagcggtga tcattgacga caaactgggg ctgaacgctc atctggaaga
    25201 agagatggcg cgcctgcgtg aagcggtagt gtgtgagtgg actgaaacgg tcaatacacc
    25261 gtccgcgcag actcgcttca aacacttcat caacagcgac aagcgtgacc cgaacgtgca
    25321 gatggtgcca gagcgcgaac agcaccgtcc ggcaacgccg tatgaacgta tcccggtaac
    25381 tctggtggag gacaacgcat gagccagtgg aaagacatct gcaaaatcga tgacatcctg
    25441 cctgaaaccg gcgtctgcgc actgttaggt gacaagcagg tagcgatttt ccgcccgtat
    25501 cacagcgacc aggtgtttgc gatcagcaac attgatccgt tcttcgagtc cagcgtgcta
    25561 tcacgcgggc tgattgcgga acaccagggc gagctgtggg tcgccagccc gctgaaaaaa
    25621 cagcgttttc gcttaagtga tggcttgtgc atggaagacg aacagttttc cgtcaaacat
    25681 tacgaagcac gagtgaaaga cggcgtggtg cagctgcgcg gttaatgttt taacgggagg
    25741 cgcaatgcct cccctttttg catggtcctg taatgacctt cggtatattg caggacattt
    25801 tttaaacttt ttgttttatt ttttgttttt attttttaaa ggataatcaa atgtttacag
    25861 acactattaa taagtgtgcg gctaatgctg cgcgcattgc acgcctgtcg gcaaataacc
    25921 cgctcggctt ttgggtcagc tccgccatgg cgggcgcata tgtgggtctt gggatcatcc
    25981 tgattttcac gctcggtaat ttgctcgacc cgtccgtacg tccgctggtg atgggcgcga
    26041 cctttggtat cgccttaacg ctggtgatta tcgccggttc tgaactgttc accggacaca
    26101 ccatgttcct cacctttggg gtaaaagcgg gcagcatcag ccacgggcaa atgtgggcaa
    26161 tcctgccgca aacctggctg ggtaacctgg tcggttccgt cttcgttgcc atgctctata
    26221 gctggggcgg cggtagcctg ctgccggtag ataccagcat cgttcactcc gtcgcgctgg
    26281 ctaaaaccac tgcaccggca atggtactgt tcttcaaagg tgcattgtgt aactggctgg
    26341 tttgcctggc aatctggatg gcgctgcgca ctgaaggggc ggcgaaattt atcgctatct
    26401 ggtggtgtct gctggcattt atcgcgtccg gctacgagca ctctatcgct aacatgacgc
    26461 tgttcgcgct ctcctggttc ggcaaccaca gcgaagccta cacgctggcg ggtattggtc
    26521 ataacctgct gtgggtgacg ctgggtaata ctttatcagg tgccgtattc atgggattgg
    26581 gttattggta tgctacgccg aaagcgaatc gtccggttgc ggacaaattt aatcaaactg
    26641 aaacagctgc cggttaatta ctaaggggtt tttacgtgga tcatttgcct atattttgcc
    26701 aattacgcga tcgcgactgt ctgattgtcg gcggtggtga tgtcgcggaa cgcaaagcaa
    26761 ggttgctgtt agacgcaggc gctcgcttaa cggtgaatgc attagcgttt attccacagt
    26821 tcaccgcatg ggcagatgca ggcatgttaa ccctcgtcga agggccattt gatgaaagcc
    26881 ttctcgacac ctgctggctg gcgattgcag cgacggatga tgacacgctt aaccagcgcg
    26941 tcagcgaagc cgctgaagct cgtcgcatct tctgtaacgt agttgatgcg ccgaaagccg
    27001 ccagctttat tatgccgtcg attattgacc gctcaccgct aatggtagcg gtctcctctg
    27061 gcggcacctc tccggttctg gcgcgcctgt tgcgcgaaaa actcgaatca ctgctgccgt
    27121 tgcatctggg ccaggtggcg aaatatgccg ggcaattacg cgggcgagtg aaacaacagt
    27181 tcgccacgat gagtgagcgt cgccgtttct gggagaaatt gttcgttaac gaccgcctgg
    27241 cgcagtcgct ggcaaacaac gatcagaaag ccattactga aacgaccgaa cagttaatca
    27301 acgaaccgct cgaccatcgc ggtgaagtgg tgctggttgg tgcaggtccg ggcgatgccg
    27361 ggctgctgac gctgaaagga ctgcaacaaa ttcagcaggc agatgtggtg gtctacgacc
    27421 gtctggtttc tgacgatatt atgaatctgg tacgccgcga tgctgatcgc gttttcgtcg
    27481 gcaaacgcgc gggataccac tgtgtaccgc aggaagagat taaccagatc ctgctgcggg
    27541 aagcgcaaaa aggcaaacgc gtggtgcggc tgaaaggtgg cgatccgttt atttttggcc
    27601 gtggtggcga agagctggaa acactgtgca acgcgggtat tccgttctcg gtggttccgg
    27661 gtattaccgc agcttctggt tgctctgcct attcgggtat tccactcacg catcgcgatt
    27721 atgcccagag cgtacgctta attaccggac acttaaaaac cggtggcgag ctggactggg
    27781 aaaacctggc ggcagaaaaa cagacgctgg tgttctatat ggggttgaat caggccgcga
    27841 ctattcagca aaagctgatt gaacacggta tgcctggcga aatgccggtg gcaattgtcg
    27901 aaaacggaac ggcagtcacg cagcgcgtga ttgacggtac gctcacgcaa ctcggtgaac
    27961 ttgctcagca aatgaacagt ccatcgctaa ttattattgg tcgggttgtt ggcctgcgcg
    28021 ataaactgaa ctggttctcc aatcattaat ttaacccggg ccagagaatt ctggccttct
    28081 taatgactct ttttatatat tcagcaaata aaacataacc ccttaataaa tattatctgc
    28141 taatgggtta tattcaccgt tacagcgact tacaaaatta agtcattcgc tcactgacgc
    28201 caattaaata ttcctgtatc gttcttaacg agcagtctgc ttatattcat ataatcaatg
    28261 aatattaatt aatatataat acatagggaa tgtaaatgaa caaatttatt aaagttgcac
    28321 tggtaggtgc agtactggct acgttaactg catgtactgg tcatattgaa aaccgtgata
    28381 agaactgctc ttacgactac ctgctgcacc cggcaatttc tatttctaaa atcattggcg
    28441 gttgcggtcc tactgcacag taagtctctg acagaaatcg gctaacaccg gtttaattgc
    28501 ccgacgccct ctggattttc cgagggcgta tttgtttcta tgtccttctt gcccccgccc
    28561 gtaaaaatat tttcacttta aattcaattt tataactaca tccattagcc acagcgcgat
    28621 cctgtgcgaa attttgtgat cttcctccac attacacaac ataatatgtt gttatattca
    28681 tcatgcattg tcatgttacc ttttaaatga ctgcaaactc tcccctacaa cgtattggac
    28741 aagaaaaagg tatcgctatg ggaagccagg aactccaacg caagctcaga ttttgggccg
    28801 ttcttgcaat cgccgtcggg acaaccgtcg gctccggtat ttttgtatct gtgggtgaag
    28861 tggcaaaagc agcgggcacg ccgtggctta cggtgctcgc gtttgtcatt ggcgggttaa
    28921 ttgtgatccc gcaaatgtgc gtctatgcgg aactatccac cgcttatccg gaaaatggcg
    28981 cagattatgt ttatctgaaa aatgccggaa gccgaccgct ggctttcctc tccggctggg
    29041 ccagcttctg ggccaacgat gcgccgtcat tgtcgattat ggcgctggcg attgtcagca
    29101 atcttggctt tttaacgcct atcgatccgt tgctcggtaa atttatcgcc gccggattaa
    29161 ttatcgcctt tatgttgcta cacctgcgct ccgttgaagg cggcgcagcg tttcagacgc
    29221 taattaccat cgccaaaatt atcccgttca ctatcgtcat tggccttggg atcttctggt
    29281 ttaaagcaga gaattttgcc gcccttacca ccactgcgat tggcgcaacg ggcagcttta
    29341 tggcgctgct ggcggggatc tctgccacca gttggtcgta taccggcatg gcctctatct
    29401 gttatatgac cggcgaaatt aaaaaccccg gaaaaaccat gccacgagcg ctgattggtt
    29461 cctgtctgct ggttctggtg ctctacaccc tgctggcgct ggtgatttcc ggcctgatgc
    29521 ccttcgacaa actcgccaat tctgaaacgc cgatttccga cgccctgacc tagatccccg
    29581 cactcggcag caccgctggg ctctttgttg ccatcacggc gatgatcgtc attcttggtt
    29641 cgctttccag ctgcgtgatg taccagccgc ggctggaata cgcgatggcg aaagacaacc
    29701 tgttctttaa atgcttcggc catgtgcatc cgaaatacaa cacgccggat gtctccatca
    29761 tcctgcaagg ggcgctgggg atcttcttca tcttcgtttc cgatctcacc agcctgctgg
    29821 gttatttcac cctggtgatg tgtttcaaaa ataccctcac cttcggctcc atcatctggt
    29881 gtcgtaaacg cgacgattac aaaccgctgt ggcgtactcc ggctttcggg ctgatgacca
    29941 ccctcgccat tgcgtcaagc ctcattctgg tcgcctcaac ctttgtctgg gcaccgattc
    30001 ccggccttat ctgcgccgtc atcgttattg ctactggtct gcctgcttac gccttctggg
    30061 cgaagcgtag ccgccagctc aacgctttgt cgtaatgtta tctgaagaaa ataaaatgtt
    30121 ggatattgat aaaagcaccg tggactttct ggtcaccgaa aatatggttc aggaagtgga
    30181 aaaagttctc agccatgacg ttccgctggt gcacgccatc gtggaagaga tggtgaagcg
    30241 cgacattgat cgtatttatt tcgttgcctg cggctcgcca ctcaacgcgg cgcaaacggc
    30301 gaaacatctg gcggatcgct tttccgatct tcaggtctac gccatttccg gctgggagtt
    30361 ctgcgataac accccgtatc gcctcgacga tcgttgcgcg gtgattggcg tttctgacta
    30421 cggtaaaacg gaagaggtaa tcaaagcgct ggagctgggc cgggcctgcg gcgcactcac
    30481 tgcggcgttc accaaacgcg cggacagccc gattacctcg gcggcggaat ttagcattga
    30541 ttatcaggcc gactgtatct gggaaattca cctgctgctc tgctacagcg tggtgctgga
    30601 gatgatcacc cgcctcgcgc cgaacgcgga aatcggcaag atcaaaaacg atctcaagca
    30661 gttgccgaat gcgctcggtc atctggtacg cacctgggaa gaaaaaggcc gccagcttgg
    30721 tgaactggcc agccagtggc cgatgattta taccgttgct gcgggtccgc tgcgtccgct
    30781 gggttacaaa gaaggcattg taacgctgat ggaatttacc tggacgcacg gctgcgtgat
    30841 tgagagcgga gagttccgcc atggcccgct ggagattgtc gaaccgggcg ttccgttcct
    30901 gttcctgctc ggcaatgatg aaagtcgcca caccaccgaa cgcgccatta actttgttaa
    30961 acagcgtact gacaacgtga tcgtcatcga ttacgccgaa atttcgcaag ggctgcaccc
    31021 gtggctggca ccgttcctga tgttcgtgcc aatggagtgg ctctgctact acctgtctat
    31081 ttacaaagat cacaacccgg atgaacgccg ctattacggt ggtctggtgg aatattaatc
    31141 cctctcccgg cccggcaacg ggccggattt acgcaaggag ttacccgatg aaaacaggta
    31201 tgtttacctg cggccaccag cggctgccta ttgaacacgc atttcgtgat gcaagcgagc
    31261 tgggttacga cggcatcgaa atttggggcg gtcgcccgca cgcgttcgcg ccggacttaa
    31321 aagcgggcgg catcaaacaa atcaaggcgc tggcgcagac gtatcagatg ccgattatcg
    31381 gttacacgcc agaaaccaac ggctatccgt ataacatgat gctgggcgat gaacatatgc
    31441 gtcgtgaaag cctggacatg atcaagctgg cgatagatat ggcaaaagag atgaacgcgg
    31501 gttatacgct gatttctgcg gcccacgcgg gctatctcac gccgcctaac gttatctggg
    31561 ggcggctggc agagaactta agcgaactgt gtgagtacgc ggaaaacatc ggcatggatc
    31621 tgatcctcga accgttaacg ccgtatgaat cgaacgtcgt gtgtaatgct aatgatgtgc
    31681 ttcatgcgct ggcgctggtg ccttcgccgc gcctgttcag catggtcgac atttgcgcgc
    31741 cgtatgtcca ggcggaaccg gtgatgagtt atttcgacaa actgggcgat aaattacgtc
    31801 atctgcatat tgtcgacagc gacggggcca gcgacacgca ttacattcct ggagaaggca
    31861 aaatgccgct gcgggaactg atgcgcgata ttattgatcg gggctatgag ggttactgta
    31921 cggtggagct ggtgacgatg tatatgaacg agcccagact ctatgcccgc caggcgctgg
    31981 aacgctttcg cgcgctgctg ccggaggatg agagatgaaa accctggcga caatcggcga
    32041 taactgcgtc gatatctacc cgcaactgaa taaagcgttt tctggcggta atgcggtcaa
    32101 tgtggcggtg tactgcactc gctacggcat acagccggga tgcattacct gggtgggtga
    32161 cgatgactac ggcacaaagc tgaagcagga tctcgcccgc atgggcgtcg atatcagcca
    32221 tgtccatacg aaacacggcg ttaccgcaca aactcaggtg gaactgcacg acaatgatcg
    32281 cgtttttggc gactacaccg aaggcgtgat ggccgacttt gccctgagtg aagaggatta
    32341 cgcctggctg gcgcagtatg acattgtgca cgcggcaatc tggggacatg cggaagacgc
    32401 attcccacag ctgcacgctg cgggcaaact taccgctttc gacttctccg acaagtggga
    32461 cagcccgctc tggcagacac tggtgccgca tctcgatttt gcctttgcct ccgcaccgca
    32521 agaagacgaa gcgctgcgtc tgaagatgaa agcgattgtt gcccgtggcg caggaacagt
    32581 gattgtcacg ctgggtgaaa acggcagcat tgcctgggat ggcgcgcagt tctggcgtca
    32641 ggctcctgaa ccggtgacgg ttatcgacac catgggtgcc ggagattcgt tcattgccgg
    32701 attcctttgc ggctggtctg cggggatgac attaccgcag gcgatggcgc agggaacggc
    32761 gtgcgcggcg aaaaccattc agtaccacgg tgcctggtag gtataacgtt ggcgtgagca
    32821 tcttcacgcc aacgtgctgt tacttgccgg aaaacgaccc tataatccga gtaattcatt
    32881 ctttatttca gggtcgatta tgtcagctac ggaccgctac tctcatcaac tcctctacgc
    32941 taccgtccgc cagcggctgc tggatgatat cgcgcagggg gtttaccagg ccgggcaaca
    33001 gatccctacc gaaaacgagc tttgtacaca atataacgtc agccgcatta ccattcgcaa
    33061 agccatcagc gacttagtgg cagacggcgt actgatccgc tggcagggaa aaggcacctt
    33121 tgtacaaagc cagaaagttg aaaacgccct gcttactgtc agtggtttta ccgattttgg
    33181 cgtctcacaa ggcaagtcga cgaaagagaa agtgatcgaa caggaacggg tcagcgccgc
    33241 gccgttttgc gaaaagctga acatccccgg aaacagcgaa gtgttccatc tctgccgggt
    33301 gatgtatctc gataaagagc cgctgtttat tgatagttca tggatcccgc tgtcgcgtta
    33361 tcctgacttt gatgagattt acgtcgaagg aagctccacc tatcagttat ttcaggagcg
    33421 ttttgacacg cgagtggtca gcgacaaaaa gaccatcgat atctttgccg ccacccgccc
    33481 gcaggcaaaa tggctgaaat gcgaactggg cgaaccgttg tttcgcatca gcaaaatcgc
    33541 ctttgatcag aatgacaaac cggtgcacgt ctccgaactc ttctgccgcg ccaatcgcat
    33601 caccttaacg attgataata aaagacatta accgtaggcc ggataagatg cgccagcatc
    33661 gcatccggcg atgctggcgc actgaatttt acatcccgca cgttcccctc accctaaccc
    33721 tctccccaaa ggggcgaggg gaccgttcag tgcggaattt agcttgattg ccgaacggtg
    33781 ctatccagca ttaaatactc gcaatactct cacgcaaaat ccgcagcacc gtctcttcac
    33841 cgctgcgatt cgggttaatg cgaatcgcac aatgttcgag ttgtggattc gcctggcgaa
    33901 acgttcctga aagtcgatag aagagcggcg ggatttcata tttcgactct gcaccgaccg
    33961 ggtaaggcaa cgcgccacgc ttttgcgctt cttccagcac cctggcggca atcagctgat
    34021 gaaactcgac aatcaacacc ttcgactgcg cattggcaat gacggcgctt ttcacttccg
    34081 ccaccgcgcc gccgttaagc aaagccagca accgttcgga tactcccgcc tgcaccgcat
    34141 gcatcactgg cgcgaacacc agaccacgca agacttccag cgcctgtgag ccctggatct
    34201 ggctaccgcc ggagtaaagc gtggcgcgaa tccgactaat aacatcggca tcgccaacca
    34261 ctgcaccaac gccctccggc ccaaacagct tgaagcagga gaatgtcgag acattcgcgc
    34321 cgcattcaca gccgattcgc gctaccttca tcaccgcata gttatcatcg gttaacgctg
    34381 gaacacctgc cgcgcgcaac gttgccagca catctgccag cacatagctg tcctgcggct
    34441 gctggcgcgt atgctgcacc agcgcggcat caggttgttg ctcgtcgacg acctgcttca
    34501 gtgccgacag gtcattgaaa tcagcagtaa taagcgtcag ccccatctgc tcaataataa
    34561 cccgtgtcgt cgggtaaaca ggcgcgtcat gcaccagaag acgctgcccc ggtttgagca
    34621 aagccgccag cccggcgcga atcgcgccag tccccgcgcc ctgcaccagc gccgcggcct
    34681 gtgcgtgaaa tgcatcagcc agcacctgct ccacccgttg ggtaatacgc ggttgattca
    34741 gacttggagt taaccctaaa tcaccgccag caagaaactc gctgcctgga aaatggcggc
    34801 aaatagtgtc caccagcgca aactgctttt gctgcgcttc agcaagagtc aggctttgca
    34861 aaggaaacgt cttcataggt gccttcctta agccggaacg aacaggccaa gccagtaaag
    34921 aacattcagc agaataccgg tgatcattac tgccacgacc ggtgccgcca ttttctgtac
    34981 cggacggccc agggattcgt tgaggaagta aatggcaacc gcgatagaga atccggtata
    35041 acccgccatc ttaattgctg cgaaaatcga accgaccagc agcgccactt ccatcagcat
    35101 attcatggcg ttacggatgt tatccgacgc attacgcacc gacgggtagc gtcccagcca
    35161 tttgccgatc gaacgaagca gcaagacttc cgccgaaata accactgcgc ctaataccgc
    35221 tgcaaccatc ggattcggcg agagatagcc caccgcataa acaaaggtaa agcccgcaac
    35281 tgcatacaca cccgttgcta aggcggtggt ggcaatcaac ggcacaaacc ccagtccgcg
    35341 cataaattct gccagagccg cctgattaat cagcgtttgc gattgttccg gcgtcacgcc
    35401 tgcggaatac gctttctcca gtgtgaagat cgaaacttcg ctaccagcaa aaatcttcat
    35461 actggcaacg gcggcaatca atgctcccac gatggcgata taaggtaagt ttttgataat
    35521 ccgcgacgtg cgctcttcaa acaccgaaag cccgctggca tcgatgtcat tttcatcacg
    35581 atgacgcagg tcgtgagtta tcgcgatccc cagcagcatc accatgccaa taaagatttc
    35641 gatggattca gggttaagat gtgggaaata gcgcacgact accacacggg tcatcagaac
    35701 aaccacggcg gcgaccagac tttgcttcca gccaaactgg tagaaaatcg ccaccagcgg
    35761 gaacagtgca aaagcagaaa ccaccggcga gcttaattcc ccgaggctac ctaatacatc
    35821 caccggcagc gcggtcagca gctggttaac tggcaacagg caagtaagga tcaacacgcc
    35881 ccagatagcg ccaagaccaa acgccatcag gctgtttatc gccagtacgc cgaggatatc
    35941 ggtaggaaga aagagtaacc atgcgttgag cagcccggtt ttcagggtaa aagagatccc
    36001 caccgaggcc acaaaaccaa tgctcagacc aaaagcgata ctccccgcct cgcgacggtt
    36061 catatagcct tcaatcagtt gcggcaggat cgggtggatg ccgtcatgaa aaacagccgc
    36121 cgagcgatgc gccagaagcg atgtcatacc cgtcaggcac gccaccacga taatctgaat
    36181 atacagatcc atagtcgtgc cttttatttc agatggttaa tcaacatcgg aatcgcgtgt
    36241 tcgacgtgct caacggaaag gccaaacgcc actttccctt cagcgatcat tttggcgata
    36301 tgctcgtctt tcgctttaat gcctggtttg gcaatggtgc aacttttgtt atagccaatc
    36361 accgcgatgg caatcgacaa tgccgcgccc gcaccggtat tacacgcgcc aatgtaataa
    36421 tctagttgcc cggatttcac cttcattgcc gcttccatgt cgttgtgaat gaaaacttca
    36481 aaacagccag gagccgtcgc ttcaatggtt tttttaatct gctcacgctg taagcctgca
    36541 acgccaatct ttttcatgag tctgtcctta ttggaaaaat tgagagggat tttcacgtaa
    36601 catcacatcc acatcggcct gactgaatcc tgactggcgc aattgcggaa taaaggtggt
    36661 caataaatag tcatagccat aaccaccatt ggcttttaaa tgggagcggc gcgtaatatc
    36721 catcgacagc atgacgcggt tcagcaaccc acggtcacgt agcgcgtgga gcatcgcaat
    36781 gcgcttttcg tccgggtagt aactgttctt accaatggtg tcgaactgca cgtacgcgcc
    36841 gagatcgatc atcttcaaaa tgttgtcgag gttgtctttc agatcgcagt gaccaacggt
    36901 gacgcgcgaa agatcaactc cgtgggcttg caacagagcc agttgttcca gccccatggt
    36961 gctgaacgac gtatgcgtgg agatcgggcg tccggtctgg ttatgcgcca gcgcagcggc
    37021 aataaatacc ttctcttcca gcggcgtaat ctttccttcg ctggagccga tctccgcgat
    37081 gatcccggct ttcagctccg tgccatcgat accctgttca atttcatcga ccatctcctg
    37141 cgccagttcc tgcacgctgc gggtcaccac atgttccggg aaaaacgcat cctggtaata
    37201 accagtacag gccaccacgt tgatcccggt ttcgcgcatt acatcgagca taaattgcgc
    37261 attgcgcccc atgtaacggt tggtcatctc aatcacatta cgtacacccc gggtcatcag
    37321 gtcgttcatc tcctggcaaa tgaacgcata ctgatcaagg cggcagtcca cgttgttttt
    37381 aaagccggag agatcaatat gcagatgctc atgggccagg gtgtaacccg tcggatcaaa
    37441 actcataggg tgtctcctgc aaaccggagg gaggaaagaa aagagcgacc attttgtggc
    37501 ggtggcgcac ggaaaaattc acacacggta gctcccacat cagaaagcgt ggtgcgcaca
    37561 ccgagctgcg tggcgaccag cccttgctga taaaccagca ctggcaccac ttcacgggta
    37621 tggtggctgt gaccaatggt cggatcgtta ccgtgatccg ccatcacgac caggcaatca
    37681 tctggctgca tcgcctcaac aagccgggca aggttacggt caacgacctg caaacgttcg
    37741 gcataacgtg cgacgtcttc tgcatgacca gcgaggtcgg tttcctggat attgatgcaa
    37801 ataaacgccg tcggatgggt gttaaattcg tcgagggtga tatccataat ccgctggcta
    37861 tccaccaggt tttgccagct cacaccataa ggattgctga cgatatctgc caccttaccc
    37921 accagcacgg ttggcacgcc cgcttcatac agtttttgcg gcacctgcac tttttcatca
    37981 acaccgtagc ccatatgcac gacctggaag ccgttgtcat aagcgccaga acgcggcgca
    38041 ttaataccaa taaagcgccc ttctttgctt tctgcggcat cgagaatacg ctgactgttg
    38101 gttaacaggc cgccaaatgt aatgacccga ccgacctgta cctggtcacg cacgatacga
    38161 ccaattttga ttgcgtcgtc aaaagagatc acagagagat tggcggtaat gttatagact
    38221 tggcctaaat ccgcctcgag attatcgcca atcgcaaccg cctgattgac cgacagaaat
    38281 tgcagatcat cgccacggcg ctccacctgc cagccagcgg aaactaatgc ctgctcaaca
    38341 cggtcaatca catcgcgaaa aggcatccgc agcggcggta acgggcgcgt gcctaaaatt
    38401 tcctgatgcc ccataaaggt atcgccacct tcatgttgca gctctgccac gccccaggtt
    38461 gcggaatctg acggctgcat atcgcctggc gcataaccca atgtgttgat tagccccagc
    38521 ttctccagcg ttggtagctg caaatgcggc aactggctca ggatgtgacc acaggtattc
    38581 gccccggcat cttgcgggcg caccagcgtg acatctttca ttgcccctac gccaaagcta
    38641 tcaatcacta acaccacaaa tcgcgccatt tatgcctcca gagggttgcc gaggctgtca
    38701 taacgaccaa ctatttctgg ttcgccgcgc tgaataccgg acaccagcac cacatcgcta
    38761 cgggtgacaa aaatctgcgt gcgaaaacag agcaccactg cactgctcac cggatactcg
    38821 cctgccagcg gcagggtgta gtcgatactg ctgtcatcca cagttttgag attggtttca
    38881 gtaatctttt gattttctgg cgtaaacacc agcgcatgtt gcgcatgacc acgacggtag
    38941 taaccgccgc cgtagcagta gctgtcgcca cggaaatgat gggagatttc acttaaccag
    39001 agcatcgcga tacgttcagg ctgatcgccc tgctggtttg ccggaatagt accggtcagc
    39061 gcatgaccgg gttcggcatg agtcgcaccg tattccgcca gcaatggcag cgaagtgcag
    39121 ctggtcgctg aaggtacgtt cagttgctca atcgcaatac cagatttcgc cagttgatcc
    39181 cgtgcttgta ccagcgtgtg aagattcggt gttggcaaaa ctttcccagc agcctcatcc
    39241 caaagcaggc aagggaaatg ggttagtccg gctaaatgca gccccggcag tttcttaatt
    39301 tcagcaatca cctcgtgcag cgaatgctgt acaaaaccgc tctcctgccc cggataaaga
    39361 aaatcatcgt cgctataaac tttgagcagc acaggctgga cccgtccggc cttaaccgct
    39421 gccgcagaaa cttcccgcgc tttgtcgaga gtaaacacgg tgatgatgtc ggtgccctgt
    39481 tcaacggcgt cagcaacctg atgacaaggg atttgtacca gatgcccctg atgcgccact
    39541 ggcaaaccag cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac aatgccgctg
    39601 tagcctaatg ccagcaattt ttccgccagc cacggattac gaccaaattg cttggtcatc
    39661 agatacagtt caatgccgta aagccgcgcc gtctcaatga gccgcttacc gttttccagt
    39721 atctgatcca cgtcgatcac ccagctgtcc ggggcgattt tgccctgctg ccacaaactt
    39781 agtgcggcgg aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa catcttgttg
    39841 cctcgtttct gttccttgaa ttaaatattc atttttttga atatttaggt tgtgcgacgt
    39901 ggatttgtcg ggagcttcaa acctcttcgt tagcagccat ccataatcca taaagattgg
    39961 ccagaaaata gccttcttca cacgggttaa cttccagagc gaattccttc aacaactcct
    40021 gatgcaattg cacaacggct tgccagtggc tggattgcgc cagttccgcc agcaactcgt
    40081 tatccagcgg ctctatttct tcaccacggc gactgcgcat cagtgcactc gccatatgtg
    40141 tcatcgccat cgttccttgc tcactgcgca ccggcagatg gcactctttt tccagtacgt
    40201 tgacgacctg catcatgcct ttgcagacgt ccttatcaat aacgcctgcc tcgcaaagca
    40261 ggttgagtct ggtttccata tcagatgcct ttcgtttaat aactgggttc ctgttcccca
    40321 ctcaccaccc gttgttgatg ggcaagcagg gcgtgtttat ctacaacagc acgcagcaat
    40381 tgttgcattg ggtaatcatc gactcgcgtc aggaccacgg cttcggtggc ctgtaaaaat
    40441 cgcggatcgt ctgttagcgg cgtcgcctct aatcccagca tggtcagttc gttttccgcc
    40501 accacgttcc agatcaccgc atcgacatcg cctttaacaa tgcgttgtaa actctcgtga
    40561 taagagagat cgactcgttc cacatcacta tcgccaaaaa agacatcggt catgattttc
    40621 tgatccgccg aacgactatc gagacctact cgcttaacat ttccagactc acctttgcgg
    40681 catatcagtt gatgttcacc aacatacgta tgcgggccaa gctcgagggc gatgcacaaa
    40741 ttgttttggc tgagataact ttccgccgcc aggcgggaaa ctactgccat gtcatagaca
    40801 ccgttaagta aacactccac gcgaatatcc gcgccgcgca tatgcgcata gtaaaaagga
    40861 atgccatcaa actgggcttt caatccgctc gctaaacctt cgtacaagcg ggtatagggc
    40921 aaaggcatcg cacataccac gttgttgata tccacatgac tcagcaatgc tttgttatcc
    40981 atctcgacaa gataactgcc attgcgccca cggcgttcaa tccgtatcgc tccgcttgat
    41041 tccagcgttt tcaacgcggc ctgcgtcagg ccaaccgatg aacggcattc agttgccagc
    41101 tcatctatgg ttttcagtcg attaccgcat ttttcaccca acaaataacg ggccagtgtc
    41161 gtgatgacga cgccttcttt tttgataaac gttcgacgca taatatattt tcaataaaat
    41221 gaatatttat atcttcatta aaatgaagat aagtggcgac gacgagatgt ggaaacggcg
    41281 aggcgcttca cattttttcg ggttgttgcg aaaagaggat aaaaaaaccg ggtttccccg
    41341 gtttcagagt gatgacgaag tgatttattc gacaatgcga gcaaagggta attcccactg
    41401 gcgcttgcct gctcccctca ccctaaccct ctccccaaag gggcgagggg accgtccgag
    41461 cgttttgata ttacggcttc gccacaaaac caatcgcttc gtacaccgct ttcagcgtac
    41521 gggaagcgtg tacactggct ttttctgcgc cgtctttcat cacctgttgc aggaaggctt
    41581 catcgttgcg gaaacggtga tagcgttcct gcaattcagt cagcataccg gaaacggcat
    41641 cagccacttc acctttcaga tgaccataca tcttgccttc gaactgtttt tccagttctg
    41701 ggatgctctg gcccgttacc gcagaaagga tatccagcag gttggaaacg cccgctttgt
    41761 tctgcacatc gtagcgaact accggcggct cgtcggagtc agtgaccgcg cgtttgattt
    41821 tcttcactac cgatttcgga tcttccagca ggccgataac gttattgcgg ttatcgtcag
    41881 acttggacat cttcttggtc ggctccagca gcgacattac gcgcgcgcca gatttcggaa
    41941 taaacggctc cggcacctta aagatctcgc catacagcgc gttgaaacgc tgggcgatat
    42001 cgcgactcag ttccaggtgc tgtttctggt cttcacccac cggtaccaga ttagtttgat
    42061 acagcaggat gtccgcagcc atcagcaccg gatagccaaa cagaccagcg ttgatgttct
    42121 cggcataacg cgcagattta tctttaaact gcgtcatgcg actcagttcg ccgaagtagg
    42181 tatagcagtt cagtgcccag cctaactgcg catgttctgg tacgtgggac tgaacaaaaa
    42241 tggtgctttt ctcaggatcg ataccacaag ccagatacaa ggccagcgta tccaacgtcg
    42301 ctttacgcag cttctgtgca tcctggcgca cggtgatcgc gtgttggtca acgatacagt
    42361 aaacgcaatg gtagtcatcc tgcatgttta cccactgacg cagcgcaccc atgtagttac
    42421 caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac gatgggctta gtcatttttc
    42481 gattcctgat tttcgctatg cggaagccct aatgcgggca gaaggtcatt tatagactga
    42541 taaattacat caggctggct gagatcgata gcctcgccgt agttgtatcc gtaggttaag
    42601 ccaactgatg ggcaacctgc cgcttttgcc gcctgaatat cattccgtga gtcgccgaca
    42661 aacagcatct gttgtggggc aattcccatc cgctcagcca ccagtaacag cgggtccgga
    42721 tgcggttttt tgttttgcac atcatcgccg ccgataacca cgctaaagta tttggcaata
    42781 tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt tgttggtgac caagcctagc
    42841 ggcaggcctt tagcctgcaa cgcgcccaac gtatcggcaa cgtgcgggaa caaaaacgtc
    42901 ccctcttcgg caacctcgcc atagtagcga tcgaacagtt tacgcagaat acgtacctgt
    42961 tcttctgccg gaatgtcgtc atcaacgggc ggtttcccca ttgttttacg cagagtcgca
    43021 cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa catctgcgcc gttaccaatc
    43081 caggtaataa cgcgttcttc acctgcgacg ggcaactcca gcgcatacag cgccatatct
    43141 accgcagcag caagaccagg agcactgtcg accagcgtac catcaagatc aaaagcgacg
    43201 ccgcgaatat cttcaaactt attcatgact tacctttgcc agttcactgc gcatttcatc
    43261 aatgactttt ttatagtctg gctggtcgaa gattgccgaa ccggcgacga acatatccgc
    43321 gcccgccgca gcgatttcgc caatgttgtt caccttcacg ccaccgtcca cttccagacg
    43381 aatgtcaaag ccagactcgt cgatacggcg acgtacttcg cgcagtttat ccagtgtttg
    43441 aggaatgaaa gactgaccgc cgaaaccagg gttgacggac atcagcagga tcacatccag
    43501 cttatccatc acgtaatcca gatagctcag aggtgtcgcc gggttaaata ccagacccgc
    43561 tttacagcca ttttctttaa tcagttgcag cgtgcggtca acatgctcgg aggcttctgg
    43621 atgaaaggta atgatgctgg caccagcggc agcgaaatca ggcacaatgc gatcgacggg
    43681 tttcaccatc agatgtacgt cgataggggc ggtaatgccg tagttacgca aggatttcag
    43741 caccattggc ccaatcgtca gattgggaac atagtggtta tccatgacgt caaaatgcac
    43801 gacatcagcg ccagctgcca gggcttttgc ggtatcttca cccaggcggg caaaatcagc
    43861 cgacagaatt gagggggcaa tcaaatactg tttcatccgc ttctccttga gaattatttt
    43921 ttcgcgggtg aaacgactcc tggtttgtac aaagccagca gttcgtccac ctttttacgt
    43981 gtgccgccgt tgctgcttat actgcgtcga actttgacga catgcaattt tgcgcgctga
    44041 taccactcac gcgttaacat cgtatcgtga ttggagatca gcactggaat atggcgctca
    44101 accagacctt cggcgatctc cgccagatgc gcttgttgtt caagcgtaaa actgtttgtg
    44161 tgatacgccg taaagttggc ggtcgcagac agcggtgcat aaggcggatc gcaatagacg
    44221 acggatgcat catctgcgcg ctccatgcta tcggcgtaag actcacaata gaaaaaggca
    44281 ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg ggaaataggg ttttttgtag
    44341 cggccaaacg gcacgttaaa ctcaccgcgc agattgtaac gacacaggcc gttgtaaccg
    44401 tagcggttca aatataaaaa cagtaccgcc cgacggaacg gatcctggct tttgttgaac
    44461 tcttcgcgga actgatagta aacctcggcg caatttgttt cgggaacaaa cagctcgcgt
    44521 gcggcctgta cgtactcatc agtacgcatc ttcacaatgt tatagagact gatcaggtcg
    44581 ctattgatat cggcaaggat ataacgagaa aagtcggtgt tgagaaacac cgacccggca
    44641 cctacaaaag gctcaaccag acattcgccc ttgggcaaat gccgtttaat atcatcaagc
    44701 aggggatact tgccccctgc ccacttcaaa aaagcgcgat ttttcttcat gccgattaac
    44761 taattacacc ttctccggct gtggagaaag ctccgacagc atccagcgct tcaggcagct
    44821 cccgcaacga taaaccattg cgggagataa ccttgattac ttcagatcgg cctgtacctg
    44881 acgcagcggt ttcgcccacg ggtttttggc ctgaacatct gctggcaatg tagataccgc
    44941 ttttttcgcc tcttctttcg aagcgtacac gccagaaacc aggacatacc acggctgacc
    45001 attacgcgtc gtttcataga caacgtagtt tttcagattc tctttcttcg cccaaccgtt
    45061 caggttgtcg tagttagagg aactgctcag ctgcagagtg taatggctgg acggtgccga
    45121 tttcaacgaa ccaacattac ctgcggtctt ccctccagcg gctggtgttg ccgtggtttg
    45181 cgccggggat gccgtctgta ctggagccgt agtcgccgtc tcttttggtg ctggcgtaga
    45241 agtcgcagcc ggtgccttcg tgctcgccac aggggcagct ggttcagtac gcttcggcgt
    45301 ctgtgctacc ggcttcggct ccgttttcac ggttgcttgc ggttttttcg gttcaatcac
    45361 cgcctgctga cgaactgggc gcgtagtgga cggacgttca gcggtttgcg ttttcgccgt
    45421 gtcacgcgat gcattgccat tgcgaacagg cgcaaccgtt gcgggttcag tcggcaacgt
    45481 ggaattgacc gccacattgt tcagctgttg ctgattttgt ggctgggtca gcgcattgtt
    45541 caggtcaccc tgcacttcaa cacgttgttg accatccgtt gccaccgggg tttgcccttg
    45601 agtcggcgta gaagagatcg gtggcagaga aacatcctgc tgagtatttt ccgcagacgt
    45661 ggttcccggc gctggctgca cgccattcgc ctgatcggtc gcattgccag caagatcaat
    45721 actcttctcg ccagacgcgg tttgatcgct ggaagtggtc gagggggctt ttagcgcaga
    45781 accgataccg atgatcaaca gcagtagaac cagaatgccg acgcccatca tcatatactg
    45841 acgagaagcg ggtttactgg ctgctttttt gcgcttacgc ggacgacgct ctacgcgctc
    45901 ttcatccacg gtttcatctt cggattcgtc aatttcttct tcgatttccg gttcctcatt
    45961 gcgctctttt tgcgcacgag tcggacggcg atcgtcagtg tcatcaagtt caatatcatc
    46021 aaaattgatc tgcggttcgc cacgttcagt acgctcagaa cgttcagaag attgacgaga
    46081 acgaccagta cgacgatcgc tgggatcggg tttcagctcg tcttctggtt tgaattcatc
    46141 catttaacac cccactaaaa ggttaatgct taccacgttg caattaacct gaagctaata
    46201 gaccgcttga taagcggcct gacctttctt gttgttacgc tgattgacaa tcggcaatgg
    46261 cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc actcttacca attgccaacg
    46321 gaagaattaa gcgcatctct cccgcaagga ctttcttgtc acgcagcata tgcggtaaat
    46381 acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa cccagcccgc gtgagcaggg
    46441 ttataatacg ctgcgtttcg gcagaactaa actgcccgag acgttccgac gtccgcgccg
    46501 ccatcaccat acccgcagcg accgcttcac catgtaacca attgccatac cccatttcag
    46561 cttcaatggc atgaccaaag gtgtgtccca gattcagtaa agcacgtaac ccggtttcgc
    46621 gctcgtcggc ggcgacaact tctgccttca gttcacaaca acggcgaata cagtacgcca
    46681 ttgccggacc gtccagacgc aacaacgcat ccagattctc ttccagccag ttaaaaaacg
    46741 caccgtcaag aataatgccg tatttgatga cttctgccag ccccgacgct aactcacgcg
    46801 ggggaagcgt tttcagacag tcgagatcca ccaccactga agcaggctgg tagaacgcgc
    46861 caatcatgtt tttaccgagg ggatggttga ccgcagtttt gccgccaacg gaggaatcga
    46921 cctgcgacag taacgtcgtc gggacttgaa tgaaacgaac accgcgctga taactcgccg
    46981 ccgcgaagcc ggtcagatcg cccactacgc cgccgccaag cgccaccagc gtagtatcgc
    47041 gaccatgcgg cttttgtaac aacgccgtaa agacggtatc gagtacagct aggcttttat
    47101 actgctcgcc gtcagggagg ataacgctat cgacgttaac acccgcctgt tcaagtacgc
    47161 cgcggacctt atcgagatac agaggagcca gggtttcgct ggtgaccaac atgacctgct
    47221 cgcccgattt cagcggtaag aatgaagctg gttcattaaa caaaccagat gcgatggtaa
    47281 ttgggtaact acgttccccg agagtaacga caatcctctc cataacgcga catccacctt
    47341 aattactgta cccgcagacg agtgtatata aagccagaat tagttgcttt ccagcatgtg
    47401 aataatctgg tttgcaacca ctttagcgct ttgatcatca gtacgaatgg tcacgtcggc
    47461 aatctcttca tacagcggat tgcgttcatt ggccaacgct tccagaactt cacgcggcgg
    47521 tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc gtgcgtgcaa gttgcttttc
    47581 gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa agacggttac gcgtttcacg
    47641 ggatttcaca gagccgccgc cagtagccag cacaataccc tgtttctcgg tcaactcatt
    47701 gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct tctaaatcga aaacccagcc
    47761 cacatcagct ccggttcgtt tctcaatctc ttgatcggaa tcgtaaaatt ccatattgag
    47821 ttgttgagct aactggcgcc caatagtgct ttttccggca cccataggcc caaccagaaa
    47881 gatattgcgt ttctctgcca ttttttcggt actactaaga ctattcgtta atgataaacc
    47941 cgcttcgctc agagagcgcc gcaggacatg aactgaaacc tcataagata ttgcgagagt
    48001 cagactgaaa attatctcaa tactccagcg ggtttggcaa ctgaataaat cacccatccc
    48061 tctgcatatc tggtcgctgc aagcgcgctg ccttgctacc accgctctgg cgataaatca
    48121 ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc tgaatgataa atcatcatca
    48181 tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt actccttgta tgctaaatac
    48241 ctgcgcgcgt caaatagatg aaacacgttc agcataaaaa cattaccgct tttacggctg
    48301 tttactcact ggaaaccagt cgtggcgtga taaacaccac taactcgcgt cgttcatctt
    48361 cttttccgtc atgacgaaat aattgcccga accagggaat gtcgccaagc aacggtacgc
    48421 tatcctgacc cgatttattt ttacgggtaa aaatgccgcc cagcgccaac gtttctccgc
    48481 tttttacctc gacctgcgtt tcgatctcct gcttatcaat cgccagcact tcgccatcgg
    48541 cctgctgtag cacctgcccc ggaacgttct ggctgatgtg taatttcagc cggatgcgac
    48601 ctttttgtaa caccgtgggc gtgacctcca tccccaggac ggcctcttta aattccaccg
    48661 acgtcgcgcc actttccccg ctggaaacct gatatggaat ttcgctctcc tgtttaatgc
    48721 tggcaggctg aagatgtgag gccagcagac gcggactggc gataatatcc agctgctgtt
    48781 tttgttcgag cgcggaaagc tcaagatcca gcaagcgtcc gttgatgcgc ccaatgttaa
    48841 aaccgacatg cgttgtcgcc gtcgctacgg agaggtcgct accaagcgtg gtgacttgcc
    48901 caacgccacc agcgtgttgc gcatcggcca gcgtccattt cacgcctaac tcacgcaaac
    48961 ttttttcatt aatagtgaca atatgcgccg acagctcaac ctgcccgacc ggcagatcca
    49021 tttgcgctac ccactgttca agcgcgctta acgccgtttt gttatctcgt agcaaaaggc
    49081 gattggtgcg tttatcgacg gtcatactcc ctttggcact cagtagcttc tcccccgctt
    49141 tcgccagttc tcccgcgtcg gcgtattgca gggttatact gcgattttcc agcggcagat
    49201 ttgcctgcgc ccgcgcctgc tccgcctcct ggcgggcgat attgtcattc tgccaggcaa
    49261 tggaatgcac tgagagaatg ttgccttcct gccgcgttat cagtccggcg cttttcacta
    49321 cagtttgtag tgcctgcttc cagggaacat ctgttagatg taacgacacc gtaccgctga
    49381 cgtctggcga cacgaccagg ttcaacttct cctgttcagc cagcgcctgc aacacctgag
    49441 ctaccggaac gtcatccacc atcagcgtca ctttttgcgg ctttgccgcc tgtacgccgg
    49501 gtatcagcat caacagtagt gcggctatcc attgcttcat ttgtatctcc ttgccgttgc
    49561 cacaaccatt gtggcggttc gcagtttgtc ccggtaccca gcgttagtac gtctggcgtc
    49621 agctgtaaaa ttgtccagcc gttttccagc acatcgtttt gctgcacccg tcgccatttc
    49681 ttttgcccgt cttttattac accgatgatg cgctcgcctc gccctaccat cccctgatag
    49741 cgccactggc taagttcgct aatccggcat agatcttccg gcgatttaaa agggtcacgc
    49801 ataccggtta aaaggcacaa tgcaatacct gccaacaacc agcgtttaac cctcatgcgg
    49861 catctccagt tgtagcgtga acaaaagatc atcaccttcc acgcttaacg aaaaacggct
    49921 cacgctgacg ttgcgctctg ccagccgtgt aaatgccgac ggcactgctt cccacagcgt
    49981 tttcaacgcc aactcgcctc cctgcgcgga tggatgccag gaaaccagtt gcgcgccgga
    50041 taactgaaaa tccagtggcg aaaagggcag cgttttttcc tcgctgaagg gagtggtgtc
    50101 taccaggcga tacagtgcgg cccactgctg gtgatgactt gcccgtagtc gaattaatgc
    50161 gtcgcgctct tcatggtgtg tcgatgatag aaaaatgagc gtaactaaca tcagcaacca
    50221 gaatgcccag caaaactggc ggaggcgggg tgatgtggcg aaccaccagt caaagaacat
    50281 gttcatcgct aaccttcctt gttaactgat actcaaattg ccagcgtccc tgcgcatcct
    50341 gctgcgtggc tccccgctga ttgagatgaa aagacgcatc ctggcggagt gacgtttcta
    50401 gtgcgtttaa cgcggtaatg cttgttgtca gccccttgat ctccagcgtt ccctgctgcc
    50461 agcttatcgt tgtcagccag gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag
    50521 attgccagtc gcgggtaaat tgtcgctggc gctgccgttg cgaacgctgt tcgcgtaact
    50581 gctgttgctc cagcaaacgt ggcttcgtta tctgtaagct gcgggcgagt tgttgttccg
    50641 cctgaagcaa aactgcgtct atgcgcgctt cggcgctgcc tgtcagacgc agtattagcg
    50701 ttatcccgac ggccagcagc agaggcgcaa cgaacatcag caaccagaaa cgcagaaaag
    50761 cggtccggcg ttgctgtcgc cagggcaaaa aattaattgg cgggttcatc agtacgcctt
    50821 cccaagcgcc agccccagcg cgatggcaaa gtctccacca ggcggcggta gcggcggctg
    50881 acgaacagaa acggcctccc aggggtcaaa tccgccttcg ccacatatcg cgacgctgtc
    50941 gggatcaacg gataacgctg ccgccagctc cttcgcgcta gtcatcccca ccgccagttt
    51001 gcgcccccag ctatagcgcg tcgcccacag ccactgttcg ttatcacgcc aggccagaca
    51061 ttgctgatga gaaggtaaaa aaggcaggaa tcgctgtaat gcactggcat ccggggtgat
    51121 cgcactaaca tgaacacgca acctttctgc cagcgtaagc agcgttgcca gctctttgct
    51181 ttgcgcggca gtcacgttat aagcggggct gagtgagtct tcgctgtaat cgaagcgcag
    51241 ggagtccgga tccatatcca gctcgcgggc catcgtccct gacagccagg ccgtttgctc
    51301 ccgctcacca agggacatcg acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg
    51361 aaacgccaac ataatgtgat gacgctgcgg cagttcgcga ctccacggta acaacgtttt
    51421 agccagctgc tgcgcatcaa caatccgccc atctttgata atgtcgtgcg ccagcggcaa
    51481 ccgccaccag cgttgcaaaa agcattcttt tgcgccccgc acgatcgcaa ccgctgccgc
    51541 ttcttgctgt tgtaaatgca aaccaatttg ccagatctta aatgtcattg tgatgatctc
    51601 cttatcaccc gtcactctga cgggtatatc aatgcgtctg gcttgccttt atactaccgc
    51661 gcgtttgttt ataaactgcc caaatgaaac taaatgggaa atttccagtg aagttcgtaa
    51721 agtatttttt gatccttgca gtctgttgca ttctgctggg agcaggctcg atttatggcc
    51781 tataccgcta catcgagcca caactgccgg atgtggtgac attaaaagat gttcgcctgc
    51841 aaattccgat gcagatttac agcgccgatg gcgagctgat tgctcaatac ggtgagaaac
    51901 gtcgtattcc ggttacgttg gatcaaatcc caccggagat ggtgaaagcc tttatcgcga
    51961 cggaagacag ccgcttctac gagcatcacg gagttgaccc ggtggggatc ttccgtgcgg
    52021 caagtgtggc gctgttctcc ggccacgcgt cacaaggggc gagtaccatt acccagcagc
    52081 tggcgagaaa tttcttcctc agtccagaac gcacgatgat gcgtaagatt aaggaagtct
    52141 tcctcgcgat tcgcattgaa cagttgctga cgaaagacga aatcctcgag ctttatctga
    52201 acaagattta ccttggttac cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg
    52261 gtaaaacggt cgaccaactg acgctgaacg aaatggcggt gatagccggg ttgccgaaag
    52321 cgccttccac ctttaatccg ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg
    52381 tactgtcgcg gatgctggat gaagggtata tcacccagca acagttcgat cagacacgca
    52441 ctgaggcgat taacgctaat tatcacgcgc cggagattgc tttctctgcg ccgtacctga
    52501 gcgaaatggt gcgccaggag atgtataacc gttatggcga aagtgcctat gaagacggtt
    52561 atcgcattta caccaccatc acccgcaaag tgcagcaggc cgcgcagcag gcggtacgta
    52621 ataacgtgct ggactacgac atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga
    52681 aagtgggcga gtcggcgtgg gataacaaca agattaccga tacgctgaag gcgctgccga
    52741 cctatggtcc gctgctgcct gccgcagtca ccagcgccaa tcctcaggga gcgacggcga
    52801 tgctggcgga cgggtcgacc gtcgcattga gtatggaagg cgttcgctgg gcgcgtcctt
    52861 accgttcgga tactcagcag ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg
    52921 gtcagcaaat ctgggttcgt caggttggcg atgcatggtg gctggcacaa gtgccggaag
    52981 tgaactcggc gctggtgtcg atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg
    53041 gctttgattt caatcagagc aagtttaacc gcgccaccca ggcactgcgt caggtgggtt
    53101 ccaacatcaa accgttcctc tacaccgcgg cgatggataa aggtctgacg ctggcaagta
    53161 tgttgaacga tgtgccaatt tctcgctggg atgcaggtgc cggttctgac tggcagccga
    53221 agaactcacc accgcagtat gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga
    53281 aaaacgtggt gatggtacgt gcaatgcggg cgatgggcgt cgactacgct gcagaatatc
    53341 tgcaacgctt cggcttcccg gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt
    53401 cagcgtcctt caccccaatg caggtggcgc gcggctacgc ggtcatggcg aacggcggct
    53461 tcctagtgga cccgtggttt atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg
    53521 aagcgaaacc gaaagtagcc tgcccggaat gcgatattcc agtgatttac ggtgatacgc
    53581 agaaatcgaa cgtgctggaa aataacgatg ttgaagatgt cgctatctcc cgcgagcagc
    53641 agaatgtttc tgtaccaatg ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga
    53701 ctggcgcgca ggagtacgca ccgcacgtca tcaacactcc gctggcattc ctgattaaga
    53761 gtgctttgaa caccaatatc tttggtgaac caggctggca gggtaccggc tggcgtgcag
    53821 gtcgtgattt gcagcgtcgc gatatcggcg ggaaaaccgg taccactaac agttcgaaag
    53881 atgcgtggtt ctcgggttac ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg
    53941 atcaccgtcg taatctcggt catacaacgg cttccggagc gattaaagat cagatctcag
    54001 gttacgaagg cggtgccaag agtgcccagc ctgcatggga cgcttatatg aaagccgttc
    54061 ttgaaggtgt gccggagcag ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg
    54121 atcgcagcac cgggcagtta gctaatggtg gcaacagccg cgaagagtat ttcatcgaag
    54181 gtacgcagcc gacacaacag gcggtgcacg aggtgggaac gaccattatc gataatggtg
    54241 aggcacagga attgttctga ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa
    54301 gtgcatttgt ttatgccgga tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa
    54361 atcaatatat tgcaggaacc ccataagcct gataagcgtt gtgcatcagg caaacttcac
    54421 gcatttacac tcgcccctgc cctttcaacc attcgcgcac gaggaacagc gcgctgacat
    54481 tacgcgcttc gttgaagtca gggtcttcca gcaaatccat catatgcgcc agcggccagc
    54541 gcacctgtgg tagcggctct ggctcatcgc cttccagcga ttccgggtag agatcttgcg
    54601 cgaccacgat attcattttg ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa
    54661 aagtcagatc gttcgcacca aatccaacct cttcttttag ctcgcggtta gcggcttcgt
    54721 agacgctttc acccggatca attaatcctt tcgaaaaacc taattcgtag gattcagttc
    54781 ccactgcgta ttcgcggatc aggatcaggt gatcgtccac aatcggcaca atcatcactg
    54841 cttcccggtt ggttggacgc attcgttcat aaacacgccg cacgccattg ctgaactcca
    54901 gatccacgct ctcgacggta aacagtcggg aacgggctac agtttcaaca ttcagaatgg
    54961 tgggtttttg taatgatttg ttcatcgtgg gatctatgct gtgaaatcag cggttattgt
    55021 gcgatatcgg acacgctttc ggcaatgtga attgcatgtt atttacattt atgtaactta
    55081 ataaataatc gtcctcaaat caaattaaaa gtcaataggt tgaaataact ccaggaattt
    55141 gctgatattc cgccttcaga gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg
    55201 atgctcaagt taagctccac gcttgccgat agccaaccgc agaatcatgt attgtgtccg
    55261 gtgcgactga ccacgcctga cagactaagt aagatgggga aagcatgagc accattgtga
    55321 tttttttagc tgctttgctg gcctgctcac tacttgcggg atggctgata aaagtgcgat
    55381 ccagacggcg tcagctgccc tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca
    55441 cacctgaaga acgtagcgcc gttgaaaatt atcttgagag cctgacgcag gtattacagg
    55501 tgcctggccc aacgggagct agcgcggcac cgatctctct ggcgctgaat gccgaaagca
    55561 acaacgtcat gatgctgaca cacgctatca cacgttacgg catctctacc gacgatccga
    55621 ataaatggcg ttactacctc gattcggtag aagtccacct gccccccttc tgggaacagt
    55681 acatcaacga tgagaatacc gttgaactga ttcataccga ttcgctgccg ttggttattt
    55741 cactcaacgg tcatacgctg caggagtaca tgcaggaaac gcgcggctat gccttgcaac
    55801 ctgttccgtc cacgcaggcg tcgattcgcg gggaagagag tgagcaaatc gagctactca
    55861 atattcgcaa agaaacgcat gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag
    55921 cgttgctgat cgtcgcctcc ttcctgatgt tctttttctg cctgattacc ccggatgtat
    55981 ttgttccgtg gctggcaggc ggcgcattac tgctgctggg cgcaggtctg tgggggctat
    56041 tcgcgccccc ggcaaaatcc tccctgcgag aaattcattg tctgcgcggt acaccccgtc
    56101 gttggggatt gtttggcgaa aacgatcagg aacagatcaa caatatttca ctcggtatta
    56161 tcgacctggt ctatcccgca cactggcagc catacattgc tcaggatctc ggtcaacaaa
    56221 ccgatattga tatctatctc gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc
    56281 atgatgaagt aaaaaacttt ccgttacagc actggctgcg cagtacgatt atcgctgcgg
    56341 gctcgctgct ggtgctgttt atgctgttat tctggatccc gctggatatg ccgctgaaat
    56401 tcactctctc atggatgaaa ggcgcgcaga ccattgaagc caccagcgta aaacaactgg
    56461 ctgacgctgg cgtgcgggta ggcgatacat tgcgtattag cggtacggga atgtgtaata
    56521 ttcgcacttc cggaacctgg agcgcgaaaa ccaattcacc ttttttaccg tttgactgct
    56581 cgcagatcat ctggaatgac gcccgctcat taccattacc agaatctgaa ctggtcaaca
    56641 aagcgacggc attgactgaa gcagttaatc gccagctgca ccctaaaccg gaagatgaat
    56701 ctcgcgtcag tgcctcattg cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg
    56761 attttggcga cattgtactg aagacagcgg atttatgttc tgccaaagat gactgtgtgc
    56821 gactgaaaaa tgcgctggtc aatctcggca acagtaaaga ctgggacgcg ctggtaaaac
    56881 gcgccaacgc cgggaagctc gatggcgtga atgtgttatt acgcccggtg agtgcggaat
    56941 cgctggataa cctggtggca acctccaccg cgccgtttat cacgcatgaa acggcgcgag
    57001 cggcacaatc actaaacagt ccggcccccg gcggattcct gattgtcagc gacgaaggca
    57061 gcgattttgt tgatcagccc tggccttcgg catcacttta cgactacccg ccgcaagaac
    57121 agtggaacgc tttccagaaa ctggcacaaa tgctgatgca tacgccgttt aacgccgaag
    57181 gtatcgtcac aaaaatcttc actgacgcca atggtacgca gcatattggc cttcatccga
    57241 tcccggatcg ttccggcctg tggcgctatc tcagcaccac attgctgcta ctgacgatgc
    57301 tgggtagcgc catttacaat ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc
    57361 gcatgatgga gattcaggcc tattatgaaa gctgcctgaa cccgcaactg atcacccctt
    57421 cagaaagcct tatcgaataa cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat
    57481 tgcttcactc tggagatttc cctcatgcat atcaacattg cctggcagga cgtagatacc
    57541 gttctgctgg atatggacgg cacgttgctc gacctcgcct tcgataacta tttctggcaa
    57601 aagctggtgc ctgaaacatg gggcgcgaaa aacggggtta cgccacagga agcgatggaa
    57661 tatatgcgcc agcaatatca cgacgtacag catacgctaa actggtactg tcttgattac
    57721 tggagtgagc aactgggtct ggatatctgt gcgatgacca ccgagatggg accgcgcgcc
    57781 gtactgcgtg aagataccat tccgtttctt gaggcactga aagccagcgg taagcagcga
    57841 attttgctca ccaatgctca tccgcacaac ctggcggtaa aacttgagca tacgggtctg
    57901 gacgcacacc ttgatttatt actttccacc cacacatttg gttatccgaa agaggatcag
    57961 cggttatggc atgcggtggc cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt
    58021 gatgacagcg aagcgattct cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc
    58081 gtgactaatc ctgattccgg gattgccgag aaacagtatc aacgccatcc gtcactgaat
    58141 gactaccgcc gcctgatccc ctcgctaatg tgaaggagac gccatgaaag agaaacctgc
    58201 tgttgaggtt cgactggata aatggctatg ggctgcccgt ttttataaaa cccgcgcgct
    58261 ggcccgtgaa atgattgaag gcggtaaggt gcattacaac gggcagcgca gcaagccgag
    58321 caaaatcgtc gagctgaatg ccacgctcac tctgcgccag ggaaatgacg aacgcacggt
    58381 gattgtaaag gcgattactg aacagcgtcg ccccgccagc gaggcagcct tgctgtatga
    58441 agagactgcg gaaagtgtag agaaacgcga aaaaatggcg atggcacgta aacttaatgc
    58501 cttaaccatg ccgcacccgg accgacgccc ggacaaaaaa gagcgccgcg acctgttacg
    58561 atttaaacac ggcgacagtg aataactgtc acctgcaaga gagatgatta tgccgcaaca
    58621 tgaccaatta catcgctatc tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt
    58681 ttcggaaacc ctgcaacaga tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt
    58741 gctggcagaa ctgctggttg cgaccagcct gttaaccgct acgctgaagt ttgatggtga
    58801 tatcaccgta cagctgcagg gcgacggtcc gatgaatctg gcggttatta acggtaacaa
    58861 taaccagcaa atgcgcggtg tggcgcgcgt gcagggcgaa attccagaaa atgccgacct
    58921 gaaaacgctg gtcggcaatg gttacgtggt gatcaccatt accccgagcg aaggcgaacg
    58981 ctatcagggc gtagttggtc tggaaggtga taccctggcg gcctgcctag aagattactt
    59041 tatgcgttct gaacagctgc cgacgcgcct gtttattcgc accggcgacg tagacggcaa
    59101 accggctgca ggcggtatgt tgttgcaggt aatgcctgcg caaaataccc aacaggacga
    59161 ctttgaccac ctggcgacgc taaccgaaac catcaaaacc gaagaactgc tgaccttacc
    59221 ggcaaacgaa gtgttgtggc gtttgtatca cgaagaagag gtgacggttt acgatccgca
    59281 ggatgtggag ttcaaatgca cctgctcgcg tgaatgttgc gccgatgcgc tgaaaacgct
    59341 gcctgatgaa gaagttgata gcatcctggc ggaagatggc gaaattgaca tgcattgtga
    59401 ttactgcggt aaccactatc tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc
    59461 gtctccggca gatccgtaag ttcattaatg agtatgtccg gcagagaggg ctctgccgga
    59521 ttttacgact taatggcctg atgtgctgcg cttaccagcg cctatgaaat aaatcgcaaa
    59581 ttattttggt cagatacagc gtcgtatccg ataaaagcgt tgtcacttac ttaagtttat
    59641 ttccagcgtt tcaccatcta ccgtcagatc gcgccacgct ttcggcctcg tccccagttt
    59701 tccatccttc gcagctgtga gtaatttttc tttcgttatt tcgttcggaa gtaaactcat
    59761 atcaagcgcg tcccattctt ttctgtctga gtcaaagcca tagacaatga ctctgtcatc
    59821 attaaaagca aacaggatcc gctccgcctg accatcacta ttcagatctt gctcgaccag
    59881 tacgcaggcg tctttttcaa tacaggtcat cacgttatag cgatcctgga ttaaggccga
    59941 ccagaatgtc gcatccggtt taacagaacc aggggcaatt aacacatttt cggcgaatac
    60001 tttttccgat acctgttcct gaagatgttg ctctccatct aacgccatca gcagatcccg
    60061 cgcgcgtttc gggtctttca taaacccggc atcgcttttc agcgactcaa gcgccgcacg
    60121 tccatagcga ccgctctgtt cgagcatgta gatagttacc tggtctggcg tgtttttgcc
    60181 gctctgataa cgcgccatat gactgttcac gctaatacgc atactgtcca gcaccggcga
    60241 attaagaagc accagtatca ccaacaccaa taacgaaacc gcaaggttca ctttgccctg
    60301 aagaacaagg ggattttgcc cattacgcca gacgatactg acaaaatacc ccagcgacca
    60361 caccagtaac accagctccg ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg
    60421 agcgacccgc agccataacc cccaggtggc gacgaacaca tacagcggag caaccagcaa
    60481 agcggtttta atcaggcaac gcaacggccc cgtccaggga agtgacgctt tttgcggatc
    60541 gcggacaata gccattaaga tcaattgcaa aaaggccagc gttaacagca acccggcggc
    60601 ggagatgtgg cgagaaatcg cgctcaggcc cgtaaacggc agggtgatga taaacatcag
    60661 ggttagcaat gatactaacg gcagcaaccc cgtggcgatt agcgtgaaca acttttgaat
    60721 agagtcgatt aaacgtgact gtgtacgcgc gaggatcacc gccagtgcgg taaccagacc
    60781 aaatgtaaga tacataaacc agtcggttgc aaaaaagagc gtattgaaaa acgtgatgcc
    60841 aacaagttta aacaactcac cccacagtaa aagcaccagc cacgtcaggc cattggagag
    60901 aaaaatcacc agtaatataa gtacgttatg ccatactgac tggtaaaagt agctatagcg
    60961 gctactgcca ttgcggatac gaaggctttg ttgtatccac ggtagcaaca acattgccat
    61021 caacagcaga tagcagccaa aatcccaaat cgccttttca gctctccagg ggttcatgcc
    61081 atcagtttgc cacttcagcc aaccgctcat ccctgtcgtg gcaataaaca ccagcgccag
    61141 ccatccccaa aggcgcttct gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa
    61201 cgcgacggtc gcaggcacgc cataaacaat ccaactgtga ttttttccgg caagccaggc
    61261 gataagcagg tagcaaacca gcccctgaag taatccggta gcaatcatcc cccagcgtgt
    61321 tgccggtgaa agttcaacgt tatccatgtt ggttatccag aatcaaaagg tgggttaatt
    61381 atcgcatccg ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg
    61441 gtgaatcgat actttaccgg ttgaatttgc atcaatttca ttcaggaatg cgattccact
    61501 cacaatattc ccaccatata aaccaagatt taaccttttg agaacatttt ccacacctaa
    61561 aatgctattt ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca
    61621 aatatgaatt tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa
    61681 agactttact attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat
    61741 ggtttgcccc cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac
    61801 aacccaagct acgacctgct gtatcaggaa gagctcgatc cgagcctgac aggttatgag
    61861 cgcggggtgt taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt
    61921 tcaccaaaag ataagtatat cgtccgtgac gataccactc gcgatacttt ctggtgggca
    61981 gacaaaggca aaggtaagaa cgacaacaaa cctctctctc cggaaacctg gcagcatctg
    62041 aaaggcctgg tgaccaggca gctttccggc aaacgtctgt tcgttgtcga cgctttctgt
    62101 ggtgcgaacc cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg
    62161 cattttgtca aaaacatgtt tattcgcccg agcgatgaag agctggcagg tttcaaacca
    62221 gactttatcg ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga acagggtctg
    62281 aactccgaaa acttcgtggc gtttaacctg accgagcgca tgcagctgat tggcggcacc
    62341 tggtacggcg gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg
    62401 aaaggtatcg cttctatgca ctgctccgcc aacgttggcg agaaaggcga tgttgcggtg
    62461 ttcttcggcc tttccggcac cggtaaaacc accctttcca ccgacccgaa acgccgcctg
    62521 attggcgatg acgaacacgg ctgggacgat gacggcgtgt ttaatttcga aggcggctgc
    62581 tacgcaaaaa ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt
    62641 cgtgatgcgc tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat
    62701 ggttcaaaaa ccgagaacac ccgcgtttct tatccaatct atcacatcga taacatcgtt
    62761 aagccggttt ccaaagcggg ccacgcgact aaggttatct tcctgactgc tgatgctttc
    62821 ggcgtgttgc cgccggtttc tcgcctgact gccgatcaaa cccagtatca cttcctctct
    62881 ggcttcaccg ccaaactggc cggtactgag cgtggcatca ccgaaccgac gccaaccttc
    62941 tccgcttgct tcggcgcggc gttcctgtcg ctgcacccga ctcagtacgc agaagtgctg
    63001 gtgaaacgta tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg ctggaacggc
    63061 actggcaaac gtatctcgat taaagatacc cgcgccatta tcgacgccat cctcaacggt
    63121 tcgctggata atgcagaaac cttcactctg ccgatgttta acctggcgat cccaaccgaa
    63181 ctgccgggcg tagacacgaa gattctcgat ccgcgtaaca cctacgcttc tccggaacag
    63241 tggcaggaaa aagccgaaac cctggcgaaa ctgtttatcg acaacttcga taaatacacc
    63301 gacacccctg cgggtgccgc gctggtagcg gctggtccga aactgtaatg atttgaagct
    63361 ggagaatatc tatccagtat cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt
    63421 tatttaccct tcttttgtcg tgccctgcgc ccgcgttacc ggcactggca gccaggcgcg
    63481 aatggaaagc ccgccccgct cgctggtgcc aagctccagc atcccgttat ggttatccac
    63541 gatacgctgc acaattgcca gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc
    63601 gcggacaaac ggctggaaca ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc
    63661 ttccacctgg aaccaggcgc gattcggctc cgttccgctg ctgactttga tccagccatt
    63721 gccataacgg gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg
    63781 cattttcact tcaatgctgc cggggtaaag cgcggtttca atttcccgct catagccact
    63841 ttcggcagca atcacctcac cgagcactgc attaagatcc gccatttcca tcggcatctc
    63901 ctgcccggtg cgcaggtagt cgataaactg ctcaatgatg gcgttgcact cttcgatatc
    63961 tttattgatc gattctgcca gatagccatc ctgctcgctc atcatctcag tcgccaggcg
    64021 aatacgcgtc agcggcgtgc gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc
    64081 atccgccagt tgcttaacac cagccgccat atggttaaag gcacgggtaa cggaacgcac
    64141 ctccgaagcg ccatattcac gcagcggcgg cggaataatc cctttaccaa cctgcaaggc
    64201 tgcgtgttcg agatcgacca acggtcggtt ctggatacga ataaacagcc acgccccgcc
    64261 tatcgccaat agcataatcg ccagcgtata gcggaacagc ggagagaaat cgccctgatg
    64321 aatttcggtc agcggcacgc gtacccagat attgggcgac agccaggttt tcagccagac
    64381 gacaggcgaa cttttgttga cctcaacgcg cacttccgtc gggccgccca gttgctgtgc
    64441 catctgatgg cttaagaatt catagtgttg cgcccaacgc agacctgcct cttcggcagc
    64501 ctcgttggag tagagagaga tccccagctc acggtagatc tcccgacgga aagcgggagg
    64561 cacaaccaac tgcgtgccgt cctccagttg cagtttgtcg gtcatcaaca tacgcacttc
    64621 gtacgcgagg actttattaa actgctggag gctcggcaaa atcgcgaagt tcagcaccac
    64681 cagataagtc gtcaccaggc tggcgaacag caaggtgacg atgagcaata acgtacgggc
    64741 aaatgaactt cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc cggtacaaag
    64801 acgtagccca ggccccaaac ggtctgaatg taacgcggat gcgctggatc ttcttctacc
    64861 atgcggcgca gacgcgaaat ctgcacgtcg atggagcgtt ccattgcgga atattcacga
    64921 ccacgggcaa ggttcatcag cttatcgcgg gagagcggct cacgcggatg gctgaccagt
    64981 gccttcagta ccgcaaactc accgctggtg agcggcatcg gctcatcttc gcggaacatt
    65041 tcgcgcgtac cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac
    65101 ggtgcgcctg gcagttcgtt cgcctgacga cgcagcaccg cacgaatacg ggccagcagt
    65161 tcacgcgggt taaacggttt tggaatgtag tcgtcagcgc caatctccag gcctacgata
    65221 cggtccactt cttccccttt cgccgtcacc ataatgatcg gcatcgggtt gctctgacta
    65281 cgaagacgtc ggcaaatcga caagccatct tcaccaggta acattaaatc cagtaccata
    65341 agatggaaag attcacgagt cagcaggcga tccatctgtt ctgcattagc gacgcttcga
    65401 acctggaagc cttgttcggt gagataacgt tccagcagcg cacgcaggcg catgtcgtca
    65461 tcgaccacca gaatcttgta gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa
    65521 tttgtaagcg tgtattctta aaaaagctca cgttcgtcac cagctaaatc tggtatgaat
    65581 ttcagcctaa attgttacaa agcatattaa acagcagctt aagtatacaa tttattcggc
    65641 gaaacattat tgattctgtt gatatgatca cgttataccc aatgtgcgca ttatcaaaca
    65701 gacaaaggga atcaacgaga tgaaaacgcc cctggttacc cgggaagggt atgaaaaact
    65761 caaacaagag cttaattatc tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac
    65821 ctgggccgca agtctgggcg accgcagcga aaatgctgac tatcagtata ataaaaagcg
    65881 tctgcgtgaa atcgaccgtc gcgtgcgcta tctcactaaa tgcctggaaa atctcaaaat
    65941 cgtcgattac tcccctcagc aggaaggcaa agtctttttt ggcgcgtggg tggagattga
    66001 aaacgacgat ggcgtgactc accgtttccg tattgtcggc tacgatgaaa tttttggccg
    66061 taaagattac atctctatcg attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg
    66121 cgatctggcg gtggtgaata cccctgccgg ggaagcgaac tggtatgtta atgctatcga
    66181 gtacgtgaaa ccgtaaggaa gagtcttaac ctcctgccga tggctggcat ttttgccagc
    66241 cagtccgtat aactatcccc tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa
    66301 tgattcgttc tgccgcatta ttgcgggtga aattcaggcg cgcccggaac aggttgacgc
    66361 tgccgttcgc ctgcttgacg aagggaatac cgtgccgttt atcgcacgtt atcgtaagga
    66421 aatcaccggc ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc tgagctatct
    66481 gcgcgagctg gaagagagac gtcaggcgat cctcaaatcc atttccgagc aaggcaaact
    66541 caccgatgat ctggcgaagg ccatcaacgc caccctaagc aaaaccgaac tcgaagacct
    66601 ctacctgccc tacaaaccta aacgccgcac ccgcgggcaa atcgccattg aagcagggct
    66661 tgagccgttg gctgacctgc tgtggagcga tccgtcacac acgccagaag tcgccgctgc
    66721 acaatatgtt gatgccgata aaggcgtggc agataccaaa gccgcgctgg atggcgcgcg
    66781 ctatatcctg atggaacggt ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta
    66841 tctgtggaag aacgcgcatt tggtttctac ggtggtgagc ggtaaagaag aggaaggggc
    66901 gaaattccgc gactatttcg atcatcacga accgttgtcc acggtgcctt ctcaccgcgc
    66961 gctggcgatg ttccgtgggc gtaacgaagg cgtactccag ctttcgctga atgccgatcc
    67021 gcagttcgat gagccgccca aagagagcta ttgcgagcaa atcatcatgg atcaccttgg
    67081 cctgcgcctg aacaatgccc cggcggatag ctggcgcaaa agcgtagtga gctggacctg
    67141 gcgcatcaag gtgctgatgc atctggaaac cgaactgatg ggtaccgtgc gcgaacgcgc
    67201 ggaagatgaa gcaatcaacg tctttgcccg taacctgcac gatctgctga tggcggcccc
    67261 tgccggactg cgtgcaacga tgggcctcga tccgggtctg cgtaccgggg taaaagtggc
    67321 ggtggtcgat gccactggca aactggtggc gaccgatacc atttacccgc acaccggaca
    67381 agccgcaaaa gcagcgatga ccgttgctgc cttgtgtgaa aaacataacg ttgaactggt
    67441 ggcgatcggc aacggtacag cttcccgcga aactgaacgt ttctatctcg acgtgcagaa
    67501 gcagttcccg aaagtgaccg cacagaaagt gatcgtcagc gaagctggcg cgtcggttta
    67561 ctcagcttcc gagctggcag cgcaggagtt cccggatctc gacgtttcgc tgcgtggtgc
    67621 ggtatctatc gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa
    67681 atctatcggc gtaggtcagt atcagcatga cgtcagccag acgcaactgg cccgcaaact
    67741 ggatgcagta gtagaagact gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt
    67801 tccgctatta acccgcgtgg cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg
    67861 gcgcgatgag aacggccagt tccagaaccg tcagcaactg ctgaaagtca gccgtctggg
    67921 gccgaaagcc ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg ataacccgct
    67981 ggacgcctct accgttcatc cggaagccta tccggtggtg gaacgcattc tggcagcaac
    68041 acagcaggca ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc
    68101 tgactttact gatgaaaaat tcggtgtgcc gacagtaact gacatcatca aagagctgga
    68161 aaaaccgggt cgcgatccgc gtccggaatt taaaaccgct cagtttgccg atggcgtcga
    68221 gacaatgaac gacctgcaac cgggtatgat cctcgaaggc gcagtgacca acgtcaccaa
    68281 ctttggcgcg tttgtcgata ttggcgtgca tcaggacggc ctggttcaca tctcttcatt
    68341 atcgaacaag tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt
    68401 gaaagtgctg gaagtggatc ttcagcgtaa acgtatcgcc ctgactatgc gtctggacga
    68461 gcagcctggc gaaaccaact ctcgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg
    68521 cccggcagcc aaaccacgcg gtcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga
    68581 tgcgctggcg gcagcgatgg gtaaaaaacg ttaaacgccc ggactggccc ctaaacctgc
    68641 gcgataaggc gttcacgccg catctggcaa cgaacgcttt gcctgacata aaagtgccgg
    68701 agaatatctc cggcactttt attccacagc caaactcaca atatactccg ttaatattta
    68761 tcacttcatt aacaactgaa accttaatta aacattagcc agtcctggta actcactatt
    68821 cgaattatat tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta
    68881 ttcaaaccaa cattcgcaca cattttaagt attgctgata gaaaccattc tcattatcat
    68941 tgtgttgttg attatttaat ctctcctttg ttggcaaatc atctggtctc atgtcgcggt
    69001 caaacgcccc atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac
    69061 actccagata ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc
    69121 caaaaactgc tttctctagg catgttacct ggctcctctt ttgatgtggt gcgcgtcgct
    69181 ccactcggcg accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa
    69241 gatctggcct tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa
    69301 aaaattaacc attggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca
    69361 gctcactggc gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga
    69421 agggcaattc tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc
    69481 tctgaccacc atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actacatttt
    69541 gagtggcgac gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct
    69601 gtacctgacg ctgcaactgc tggaactcgg cattccctgc atcgtggcgc tgaacatgct
    69661 cgacattgcc gagaagcaaa atattcgtat tgaaattgat gctctatcgg cacgtcttgg
    69721 ctgcccggtg atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc
    69781 gattgatcgc tataaagcta acgagaatgt ggaactggtg cattacgcac agccgctact
    69841 caacgaagca gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg
    69901 ctggctgggc ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc
    69961 gtcgcagcat ctggatgcca ccctcgcccg tctgcgtaat gaaatggatg atccggcgct
    70021 gcacattgcc gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac
    70081 cctgacggca gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt
    70141 cctcggtctg ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat
    70201 cggcggggcg ttacagccgc tgtttaacgt cggctccgtg gcgctatttg tgcatggtat
    70261 tcaatggatt ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg
    70321 cctgggtggc ggtattaaca ccgtgctgcc actggtgccg cagatcggca tgatgtacct
    70381 gttcctctcc ttccttgagg actccggcta tatggcgcgt gcagcgtttg tgatggaccg
    70441 cctgatgcag gcgctgggct tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg
    70501 ttgtaacgta ccgtcggtaa tgggcgcacg tacgcttgat gcaccgcgtg aacgtctgat
    70561 gaccatcatg atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt
    70621 cgcggctgcc ttcttcgggc agaacggtgc cctggcggtt ttctcgctgt atatgctcgg
    70681 tatcgtgatg gcggtgctga ctggcctgat gctcaagtac accatcatgc gcggtgaagc
    70741 gacgccgttt gtcatggagc tgccggtcta ccatgtacca cacgttaaaa gcctgattat
    70801 ccagacctgg cagcgtctga agggcttcgt tctgcgtgct ggtaaagtga ttatcatcgt
    70861 cagcattttc ctgagcgctt tcaacagctt ctcgttgagc gggaaaatcg tcgataacat
    70921 caacgactcg gcgctggctt ccgtcagccg ggtgataacc cctgtcttca agccgattgg
    70981 cgtacatgaa gataactggc aggcaacggt tggcctgttt acgggcgcaa tggcaaaaga
    71041 agtggtggtg ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt
    71101 caatccggcg gaattcaacc tcggtgaaga gctgttcagt gcggtagatg aaacctggca
    71161 gagcctgaaa gacaccttca gccttagcgt actgatgaac cccattgaag ctagcaaagg
    71221 caacggtgaa atgggtaccg gggcgatggg cgtgatggat cagaaattcg gtagcgcagc
    71281 tgccgcatac agctacctga ttttcgtcct gctgtatgta ccatgtatct cggtgatggg
    71341 agccatcgcc cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa
    71401 tatcgcttac tcactggcaa cattgttcta tcaggtcgcc agctacagtc agcacccaac
    71461 ttacagcctg gtgtgcattc tggcggtaat cctgtttaac atcgtagcta tcggtctgct
    71521 gcgccgcgcg cgtagccggg tggatatcga actgctggca acccgcaagt cggtaagcag
    71581 ttgctgcgca gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtacgt
    71641 gatttgctgg cgttacgggg ccgtatggaa gcgacccaga taagccagac attgaacact
    71701 ccgcagccaa tgattaacgc catgctgcaa cagctggaaa gcatgggcaa agccgtgcgg
    71761 attcaggaag aacctgacgg ctgcctctct ggcagttgta aaagctgccc ggaaggcaaa
    71821 gcctgtctgc gcgagtggtg ggcgctgcgt taaccatact ccatcgcctg ttttgaaaag
    71881 cccggtatgc gtttgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaattct
    71941 tgcagcgcaa cgtaagttcc tctgaaagcg tctcgcaggg atgaaaactc gctaatgcac
    72001 agatgtggag tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga acgccatgag
    72061 taaaaagcag agttccaccc cacatgatgc gctgttcaaa ctctttttac gccaaccgga
    72121 gacggcacgc gattttcttg cgtttcattt accggcaccc attcacgcgc tctgtgatat
    72181 gaaaaccctc aagctggagt cgaggagctt tattgatgac gatctgcgtg aaagctattc
    72241 cgatgtgctg tggtcggtga aaacggaaca aggaccagga tacatctatt gtctgattga
    72301 acatcaaagc acctcaaaca aactgatcgc atttcgcatg atgcgttacg ctattgccgc
    72361 aatgcaaaat caccttgatg ctggatacaa aacgttgccg atggtggtgc cattgttgtt
    72421 ttaccacggt attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga
    72481 tcccaaactg gcaaggcagc tttatgcctc cgcatttccg ctgattgatg tcaccgtcat
    72541 gcctgatgat gaaatcatgc agcaccgacg tatggcgctg ctggagttaa ttcaaaaaca
    72601 tattcgtcaa cgcgatctga tggggctggt agagcaaatg gcctgcttat taagtagtgg
    72661 atacgctaat gacagac
//
LOCUS       DAJVPP010000004        70085 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVPP010000004 DAJVPP010000000
VERSION     DAJVPP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.004"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..407
                     /locus_tag="OQM34_000270"
     CDS             <1..407
                     /locus_tag="OQM34_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1450851.1"
                     /translation="VPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARF
                     NANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQSWTVR
                     WGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            418..1509
                     /locus_tag="OQM34_000271"
     CDS             418..1509
                     /locus_tag="OQM34_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1450852.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MHAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1552..2412)
                     /gene="rsmI"
                     /locus_tag="OQM34_000272"
     CDS             complement(1552..2412)
                     /gene="rsmI"
                     /locus_tag="OQM34_000272"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1450853.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2477..4513
                     /gene="lpoA"
                     /locus_tag="OQM34_000273"
     CDS             2477..4513
                     /gene="lpoA"
                     /locus_tag="OQM34_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1450854.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4471..4866
                     /locus_tag="OQM34_000274"
     CDS             4471..4866
                     /locus_tag="OQM34_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1450855.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4886..5476
                     /gene="diaA"
                     /locus_tag="OQM34_000275"
     CDS             4886..5476
                     /gene="diaA"
                     /locus_tag="OQM34_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1450856.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5486..6061
                     /gene="yraP"
                     /locus_tag="OQM34_000276"
     CDS             5486..6061
                     /gene="yraP"
                     /locus_tag="OQM34_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1450857.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6175..7215)
                     /locus_tag="OQM34_000277"
     CDS             complement(6175..7215)
                     /locus_tag="OQM34_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1450858.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7288..7923)
                     /locus_tag="OQM34_000278"
     CDS             complement(7288..7923)
                     /locus_tag="OQM34_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1450859.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8051..8569
                     /gene="yhbO"
                     /locus_tag="OQM34_000279"
     CDS             8051..8569
                     /gene="yhbO"
                     /locus_tag="OQM34_000279"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1450860.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8549..8992)
                     /locus_tag="OQM34_000280"
     CDS             complement(8549..8992)
                     /locus_tag="OQM34_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1450861.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9043..9345
                     /gene="yhbQ"
                     /locus_tag="OQM34_000281"
     CDS             9043..9345
                     /gene="yhbQ"
                     /locus_tag="OQM34_000281"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1450862.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9332..9835)
                     /locus_tag="OQM34_000282"
     CDS             complement(9332..9835)
                     /locus_tag="OQM34_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1450863.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9829..10353)
                     /locus_tag="OQM34_000283"
     CDS             complement(9829..10353)
                     /locus_tag="OQM34_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1450864.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10562..11557
                     /locus_tag="OQM34_000284"
     CDS             10562..11557
                     /locus_tag="OQM34_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1450865.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11566..12444
                     /locus_tag="OQM34_000285"
     CDS             11566..12444
                     /locus_tag="OQM34_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1450866.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12525..13532
                     /locus_tag="OQM34_000286"
     CDS             12525..13532
                     /locus_tag="OQM34_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS1450867.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13650..14894)
                     /gene="mtr"
                     /locus_tag="OQM34_000287"
     CDS             complement(13650..14894)
                     /gene="mtr"
                     /locus_tag="OQM34_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1450868.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15048..16937)
                     /gene="deaD"
                     /locus_tag="OQM34_000288"
     CDS             complement(15048..16937)
                     /gene="deaD"
                     /locus_tag="OQM34_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1450869.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16930..17010)
                     /gene="yrbN"
                     /locus_tag="OQM34_000289"
     CDS             complement(16930..17010)
                     /gene="yrbN"
                     /locus_tag="OQM34_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1450870.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17117..18001)
                     /gene="nlpI"
                     /locus_tag="OQM34_000290"
     CDS             complement(17117..18001)
                     /gene="nlpI"
                     /locus_tag="OQM34_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1450871.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18110..20245)
                     /gene="pnp"
                     /locus_tag="OQM34_000291"
     CDS             complement(18110..20245)
                     /gene="pnp"
                     /locus_tag="OQM34_000291"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1450872.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20492..20761)
                     /gene="rpsO"
                     /locus_tag="OQM34_000292"
     CDS             complement(20492..20761)
                     /gene="rpsO"
                     /locus_tag="OQM34_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1450873.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20910..21854)
                     /gene="truB"
                     /locus_tag="OQM34_000293"
     CDS             complement(20910..21854)
                     /gene="truB"
                     /locus_tag="OQM34_000293"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1450874.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21854..22255)
                     /gene="rbfA"
                     /locus_tag="OQM34_000294"
     CDS             complement(21854..22255)
                     /gene="rbfA"
                     /locus_tag="OQM34_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1450875.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22419..25067)
                     /gene="infB"
                     /locus_tag="OQM34_000295"
     CDS             complement(22419..25067)
                     /gene="infB"
                     /locus_tag="OQM34_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1450876.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25092..26579)
                     /gene="nusA"
                     /locus_tag="OQM34_000296"
     CDS             complement(25092..26579)
                     /gene="nusA"
                     /locus_tag="OQM34_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1450877.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYGQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26607..27059)
                     /gene="rimP"
                     /locus_tag="OQM34_000297"
     CDS             complement(26607..27059)
                     /gene="rimP"
                     /locus_tag="OQM34_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1450878.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27266..27342)
                     /locus_tag="OQM34_000298"
     tRNA            complement(27266..27342)
                     /locus_tag="OQM34_000298"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27306..27308),aa:Met,seq:cat)
     gene            27690..29033
                     /gene="argG"
                     /locus_tag="OQM34_000299"
     CDS             27690..29033
                     /gene="argG"
                     /locus_tag="OQM34_000299"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1450879.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29041..30648)
                     /locus_tag="OQM34_000300"
                     /pseudo
     CDS             complement(29041..30648)
                     /locus_tag="OQM34_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31126..31212)
                     /locus_tag="OQM34_000301"
     tRNA            complement(31126..31212)
                     /locus_tag="OQM34_000301"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31176..31178),aa:Leu,seq:gag)
     gene            complement(31227..31559)
                     /gene="secG"
                     /locus_tag="OQM34_000302"
     CDS             complement(31227..31559)
                     /gene="secG"
                     /locus_tag="OQM34_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1450880.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(31787..33124)
                     /gene="glmM"
                     /locus_tag="OQM34_000303"
     CDS             complement(31787..33124)
                     /gene="glmM"
                     /locus_tag="OQM34_000303"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1450881.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33117..33965)
                     /gene="folP"
                     /locus_tag="OQM34_000304"
     CDS             complement(33117..33965)
                     /gene="folP"
                     /locus_tag="OQM34_000304"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1450882.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34055..35989)
                     /gene="ftsH"
                     /locus_tag="OQM34_000305"
     CDS             complement(34055..35989)
                     /gene="ftsH"
                     /locus_tag="OQM34_000305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1450883.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36089..36718)
                     /gene="rlmE"
                     /locus_tag="OQM34_000306"
     CDS             complement(36089..36718)
                     /gene="rlmE"
                     /locus_tag="OQM34_000306"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1450884.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            36844..37137
                     /gene="yhbY"
                     /locus_tag="OQM34_000307"
     CDS             36844..37137
                     /gene="yhbY"
                     /locus_tag="OQM34_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1450885.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37293..37769)
                     /gene="greA"
                     /locus_tag="OQM34_000308"
     CDS             complement(37293..37769)
                     /gene="greA"
                     /locus_tag="OQM34_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1450886.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38017..39450
                     /gene="dacB"
                     /locus_tag="OQM34_000309"
     CDS             38017..39450
                     /gene="dacB"
                     /locus_tag="OQM34_000309"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1450887.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVVSYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(39636..40808)
                     /gene="cgtA"
                     /locus_tag="OQM34_000310"
     CDS             complement(39636..40808)
                     /gene="cgtA"
                     /locus_tag="OQM34_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1450888.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(40824..41789)
                     /locus_tag="OQM34_000311"
     CDS             complement(40824..41789)
                     /locus_tag="OQM34_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1450889.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(41916..42173)
                     /gene="rpmA"
                     /locus_tag="OQM34_000312"
     CDS             complement(41916..42173)
                     /gene="rpmA"
                     /locus_tag="OQM34_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1450890.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42194..42505)
                     /gene="rplU"
                     /locus_tag="OQM34_000313"
     CDS             complement(42194..42505)
                     /gene="rplU"
                     /locus_tag="OQM34_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1450891.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            42763..43734
                     /gene="ispB"
                     /locus_tag="OQM34_000314"
     CDS             42763..43734
                     /gene="ispB"
                     /locus_tag="OQM34_000314"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1450892.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            43961..44239
                     /gene="sfsB"
                     /locus_tag="OQM34_000315"
     CDS             43961..44239
                     /gene="sfsB"
                     /locus_tag="OQM34_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1450893.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44287..45546)
                     /gene="murA"
                     /locus_tag="OQM34_000316"
     CDS             complement(44287..45546)
                     /gene="murA"
                     /locus_tag="OQM34_000316"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1450894.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45601..45855)
                     /gene="ibaG"
                     /locus_tag="OQM34_000317"
     CDS             complement(45601..45855)
                     /gene="ibaG"
                     /locus_tag="OQM34_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1450895.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46015..46308)
                     /gene="mlaB"
                     /locus_tag="OQM34_000318"
     CDS             complement(46015..46308)
                     /gene="mlaB"
                     /locus_tag="OQM34_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1450896.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46308..46943)
                     /gene="mlaC"
                     /locus_tag="OQM34_000319"
     CDS             complement(46308..46943)
                     /gene="mlaC"
                     /locus_tag="OQM34_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1450897.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(46962..47513)
                     /gene="mlaD"
                     /locus_tag="OQM34_000320"
     CDS             complement(46962..47513)
                     /gene="mlaD"
                     /locus_tag="OQM34_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1450898.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47518..48300)
                     /gene="mlaE"
                     /locus_tag="OQM34_000321"
     CDS             complement(47518..48300)
                     /gene="mlaE"
                     /locus_tag="OQM34_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1450899.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48308..49117)
                     /gene="mlaF"
                     /locus_tag="OQM34_000322"
     CDS             complement(48308..49117)
                     /gene="mlaF"
                     /locus_tag="OQM34_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1450900.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49327..50292
                     /locus_tag="OQM34_000323"
     CDS             49327..50292
                     /locus_tag="OQM34_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1450901.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50306..51292
                     /gene="kdsD"
                     /locus_tag="OQM34_000324"
     CDS             50306..51292
                     /gene="kdsD"
                     /locus_tag="OQM34_000324"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1450902.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51313..51879
                     /gene="kdsC"
                     /locus_tag="OQM34_000325"
     CDS             51313..51879
                     /gene="kdsC"
                     /locus_tag="OQM34_000325"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1450903.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            51876..52451
                     /gene="lptC"
                     /locus_tag="OQM34_000326"
     CDS             51876..52451
                     /gene="lptC"
                     /locus_tag="OQM34_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1450904.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52420..52977
                     /gene="lptA"
                     /locus_tag="OQM34_000327"
     CDS             52420..52977
                     /gene="lptA"
                     /locus_tag="OQM34_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1450905.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            52984..53709
                     /gene="lptB"
                     /locus_tag="OQM34_000328"
     CDS             52984..53709
                     /gene="lptB"
                     /locus_tag="OQM34_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1450906.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            53757..55190
                     /gene="rpoN"
                     /locus_tag="OQM34_000329"
     CDS             53757..55190
                     /gene="rpoN"
                     /locus_tag="OQM34_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1450907.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55213..55500
                     /gene="hpf"
                     /locus_tag="OQM34_000330"
     CDS             55213..55500
                     /gene="hpf"
                     /locus_tag="OQM34_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1450908.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55618..56109
                     /gene="ptsN"
                     /locus_tag="OQM34_000331"
     CDS             55618..56109
                     /gene="ptsN"
                     /locus_tag="OQM34_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1450909.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56155..57009
                     /gene="rapZ"
                     /locus_tag="OQM34_000332"
     CDS             56155..57009
                     /gene="rapZ"
                     /locus_tag="OQM34_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1450910.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57006..57278
                     /gene="npr"
                     /locus_tag="OQM34_000333"
     CDS             57006..57278
                     /gene="npr"
                     /locus_tag="OQM34_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1450911.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57491..58123
                     /gene="yrbL"
                     /locus_tag="OQM34_000334"
     CDS             57491..58123
                     /gene="yrbL"
                     /locus_tag="OQM34_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1450912.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58120..58847)
                     /gene="mtgA"
                     /locus_tag="OQM34_000335"
                     /pseudo
     CDS             complement(58120..58847)
                     /gene="mtgA"
                     /locus_tag="OQM34_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(58844..59497)
                     /gene="elbB"
                     /locus_tag="OQM34_000336"
     CDS             complement(58844..59497)
                     /gene="elbB"
                     /locus_tag="OQM34_000336"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1450913.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(59727..62063)
                     /gene="arcB"
                     /locus_tag="OQM34_000337"
     CDS             complement(59727..62063)
                     /gene="arcB"
                     /locus_tag="OQM34_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1450914.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62159..63088)
                     /locus_tag="OQM34_000338"
     CDS             complement(62159..63088)
                     /locus_tag="OQM34_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1450915.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDVVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            63763..68223
                     /gene="gltB"
                     /locus_tag="OQM34_000339"
     CDS             63763..68223
                     /gene="gltB"
                     /locus_tag="OQM34_000339"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1450916.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLLFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68236..69654
                     /gene="gltD"
                     /locus_tag="OQM34_000340"
     CDS             68236..69654
                     /gene="gltD"
                     /locus_tag="OQM34_000340"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1450917.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            69838..>70085
                     /locus_tag="OQM34_000341"
     CDS             69838..>70085
                     /locus_tag="OQM34_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450918.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVS"
BASE COUNT        17157 a        19024 c        17412 g        16492 t
ORIGIN      
        1 tggttcctta tctcacgact tatcaggaaa accgtctctc ggtagatact acgcagctgc
       61 ccgataacgt cgatcttgaa caaacaacac agtttgtggt gcccaacaga ggtgcaatgg
      121 tagcggcgcg tttcaacgcc aatatcggtt atcgcgtact tgttacagtc agcgatcgca
      181 acggtaaacc gttgcccttt ggcgctcttg ccagcaacga tgatacgggg caacaaagta
      241 tcgtcgatga gggcggcata ctatatctct ctgggatatc gagtaaatca caaagctgga
      301 ctgtacgctg gggaaatcag gcagatcaac aatgtcagtt tgcttttagt acaccggatt
      361 cagaaccaac aacctctgta ttacaaggca cagcgcagtg ccattaagga taaaaaaatg
      421 aaaagagcgc ctcttataac aggacttttg ttgatatcca catcctgcgc ttatgcctcc
      481 tcaggagggt gtggagccga cagcactagc ggtgcgacaa attacagcag tgtggttgat
      541 gatgttacgg tgaaccagac agataacgtg acaggacggg agtttacctc tgcaacgcta
      601 agtagcacta actggcaata cgcctgttcc tgctctgcgg gtaaggcagt taaacttgtc
      661 tatatggtca gccccgtact taccaccact ggacatcaga caggatatta caaactcaat
      721 gacagcctgg atattaaaac cacattacag gcaaacgaca ttccaggact cacaaccgac
      781 caggttgtct ctgttaacac ccgattcaca cagataaaaa gcagcaccgt atattctgct
      841 gcaacccaaa cgggtgtttg ccagggtgac acgtctcgtt atggacccgt taatattggt
      901 gcaaatacca cctttaccct gtatgtcacc aagccatttc tcggctcgat gaccattccg
      961 aaaacggata ttgccgtcat taaaggcgcg tgggtcgatg gaatgggaag cccgtctaca
     1021 ggtgacttcc atgatttagt caagttatcg attcagggaa atctcaccgc cccacagtcg
     1081 tgcaaaatta atcagggcga tgttattaaa gttaattttg gattcatcaa tggtcagaag
     1141 tttaccaccc gcaatgccat gccagacggt tttactccag tagactttga tatcacttat
     1201 gactgtggtg atacttcaaa gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact
     1261 ggggtagtag accagtacaa cctggtcgcc aggcgaagaa gttcagacaa tgtgcccgat
     1321 gtcggtattc gtattgaaaa tctcggcggc ggagttgcaa atattccttt tcagaacggt
     1381 atccttcccg ttgatccttc cgggcatggc accgtcaata tgcacgcctg gccagttaat
     1441 ctggtcggtg gtgagctgga aacaggaaaa tttcagggca cagccaccat taccgtcatg
     1501 gtgcggtaaa agaagacgtt acgttttgat taacgcaacg cttcggcact gttacccctg
     1561 ctgctccagc gcatacttat acagcgcatt tttcttcacg ccatgaattt ctgcggccag
     1621 cgccgccgct tttttcagcg gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc
     1681 ggcgggtaag tcttcttcct gtgctttatg accttcgaca atcagcacca tttcgccttt
     1741 gcgacggttt tcatcttcct ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat
     1801 ggtttcccag gttttggtca gctcacgcgc cagaaccacg tagcgggatt cgcctaatac
     1861 cgcaacgata tcttccaggc tatctaacag acggtgggta gattcataaa aaatcagcgt
     1921 gcgcggctcc gcttcaatgg cttttagcgc atcacggcgg ccttttgatt tggcaggtaa
     1981 aaagccttcg taacagaaac ggtcagatgg taaacccgcg gcgcttaacg cagtgatagc
     2041 agcacacggt ccgggtagcg gcaccacacg aatccccgct tcacggcagg tacgcaccag
     2101 atggtagcca ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg caatgttttg
     2161 cccctcttgc agctttgcca gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag
     2221 cgcaaacaac cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc
     2281 ctcggcggca atcagatcaa cggcctgtaa tacctctaac gtacgctggg tgatatccgc
     2341 cagattgccg attggcgtcg gtacaatgta aagctggccc tgagaattat ccgccgattg
     2401 gtgttgtttc attgtgtcgt ccgtattgcc gatttaatat tgagcattgc gtaaaaaaaa
     2461 tatcactgga tacattatgg taccctcaac attttctcgt ttgaaagccg cgcgttgtct
     2521 gcctgttgtt ctggcagccc tgattttcgc cggttgtggc acccatactc ccgatcagtc
     2581 cactgcttat atgcagggca cggcgcaggc tgattctgcc ttttatcttc agcagatgca
     2641 gcaaagctct gatgatacca ggatcaactg gcaattactc gccattcgtg cactggtgaa
     2701 agaaggtaaa accgggcagg cggttgagtt gtttaaccaa ctaccgcaag aactgaacga
     2761 ttctcagcgt cgcgagaaaa cactgctggc ggcagagatt aaactggcgc agaaagattt
     2821 tgctggcgcg caaaacttgc tggcgaaaat cacacctgcc gatttagaac aaaaccagca
     2881 agcgcgttac tggcaggcaa aaatcgatgc cagccagggg cgtccttcca ttgatttact
     2941 gcgcgcgtta attgctcagg aaccgctgct tggcgcgaaa gaaaaacagc agaatattga
     3001 tgccacctgg caggcgctct cctccatgac tcaggaacag gcgaatacgc tggtgatcaa
     3061 cgccgacgaa aatattctgc aaggctggct ggatctgcag cgcgtctggt ttgataaccg
     3121 taacgatccc gacatgatga aagccgggat cgccgactgg cagaaacgtt atccgaacaa
     3181 tccgggcgcg aaaatgctgc caacgcagtt ggttaacgta aaagcgttta aaccagcctc
     3241 gaccaacaaa atcgccctgc tgttgccgct gaatggccag gcagcggtat ttggtcgcac
     3301 tattcagcaa ggctttgaag ccgcgaaaaa tatcggcact cagccagtgg cggctcaggt
     3361 agctgccgca cctgccgcag acgtagcaga acaacctcag ccgcaaactg cggatagcgt
     3421 tgccagcccg gcacaagcct cggttagcga tctgaccggt gatcagcctg cggcccagcc
     3481 ggtgcctgta agcgccccgg cgacaagcac cgcagcggta agcgcacccg caaatccatc
     3541 cgcagagctg aaaatctacg atacctcatc acaaccactt agccagatct taagccaggt
     3601 tcagcaggat ggcgcgagta ttgtggttgg tccgttgctg aaaaataacg ttgaagagtt
     3661 gctgaagagc aacaccccgc tgaacgtact ggcactgaac cagccggaga atatcgaaaa
     3721 ccgcgtcaat atttgttact tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg
     3781 tcatattcgt gaccagggta aacaagcgcc gctggtgctg atcccacgca gtgcattggg
     3841 cgatcgcgta gccaatgcgt ttgcgcaaga gtggcagaaa ctgggcggcg gcaccgttct
     3901 gcaacaaaaa tttggttcca ccagcgaatt acgcgcgggt gttaacggcg gttctggtat
     3961 tgctttaacg ggtagcccga ttactcccag agcgacaacc gactccggca tgacgaccaa
     4021 caatccaacg ctgcaaacca cgccaaccga tgaccagttc accaataatg gcggtcgtgt
     4081 cgatgcggtc tacattgtgg caacgccggg tgaaatcgct tttatcaaac cgatgatcgc
     4141 catgcgtaac ggtagccaga gcggtgcaac gctgtacgcc agttcccgca gcgcacaagg
     4201 caccgcaggc ccggatttcc gactggagat ggaaggcttg cagtacagcg aaatcccgat
     4261 gctggcaggc ggtaatctgc cgttaatgca gcaggcgctc agcgcggtga acaacgatta
     4321 ttcgctggct cgcatgtatg cgatgggcgt tgatgcctgg tcgttggcaa atcatttctc
     4381 acaaatgcgc caggttcagg gttttgaaat caacggtaat accggaagtc tgacggctaa
     4441 cccggattgc gtgattaaca ggaagttatc atggctacag taccaacaag gtcaggtagt
     4501 ccccgccagt taaccaccaa acagaccggc gatgcgtggg aagcacaagc gcgtcgctgg
     4561 ctggaaggca aaggactgcg gtttatcgcc gctaacgtga acgagcgtgg cggcgagatc
     4621 gatctgataa tgcgtgaagg cctgaccacc gtttttgtcg aggtacgcta tcgccgctct
     4681 gcgctttatg gcggcgcggc agccagtgtg acccgcagca aacaacacaa attattacag
     4741 actgcccgct tgtggctcgc gcgtcataat gggagttttg atactgtgga ttgccggttc
     4801 gatgtggtag ccttcaccgg gaatgaggtt gagtggatta aggatgcctt taatgaccac
     4861 tcataattaa ggtttaagga ttagcgtgca agaaagaatt aaagcttgct tcactgaaag
     4921 cattcaaact caaattgcgg cggcagaggc gcttccggat gccatctccc gtgcagccat
     4981 gacgctggtt cagtctctgc tcaatggcaa caaaatcctc tgttgtggta atggaacttc
     5041 cgctgccaat gcacagcatt ttgctgccag catgatcaac cgtttcgaaa cggagcggcc
     5101 cagcttacct gccattgcac taaatactga taatgttgtc ttaacggcga ttgccaacga
     5161 tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt
     5221 attgttagcc atttccaccc gtggcaacag ccgcgatatt gttaaagcag ttgaagccgc
     5281 cgttacgcgt gatatgacca ttgtggcatt gaccggctat gacggcggcg aacttgcagg
     5341 tttgttaggg ccacaggatg tggagatccg cattccttcg catcgtagtg ctcgcattca
     5401 ggaaatgcat atgctgacgg taaattgcct gtgcgatctg atcgataaca cgcttttccc
     5461 tcaccaggat gattaaggag aatacatgaa ggcattatcg ccaatcgcag tccttatttc
     5521 cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg ggtaccgctg ctgtgggtac
     5581 caaagccgca actgacccac gcagtgtcgg cacccaggtg gacgatggta ccctggaagt
     5641 gcgcgtgaac agcgcattgt cgaaagacga acagattaag aaagaaaccc gtattaatgt
     5701 cacggcctat cagggcaaag tgctgctggt tgggcagtca ccaaatgctg aactttcggc
     5761 tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac gaagtgtata acgagattcg
     5821 tcagggccag ccgattggtc tgggcgaagc atctaacgat acgtggatca ccaccaaagt
     5881 gcgttcgcag ctcttaacca gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga
     5941 aaacggtgaa gtgttcctga tggggctggt gactgaacgt gaagcgaaag cggcggcaga
     6001 tattgccagc cgggtgagcg gcgtaaagcg ggttaccaca gcgtttacgt ttattaaata
     6061 gcagcccctt gtaatgcctg atgcgacgct tgccgcgtct tatcaggcct acaacgatgc
     6121 aaaccgtagg tcggataagg cgtttacgcc gcatccgaca tgttttccct caaatcagaa
     6181 caacagcgcc agcccgccga caatcacacc agacactgcc accatcgccc ctgtcagcca
     6241 taaggctttc gccgggaacg ctttgcgcag cataatcagt gacggcaaac tcactgccgg
     6301 gagcgtcatc aacaatgcca gcgccggagc ggttcccata cctgccagca tcatcgtttg
     6361 tacaatcgga atttctgctg ccgtgggaat gacaaacaag catcctgcta ccgccatcgc
     6421 caccacccac atcaggctgt tatcgacagc accatcggca tgggggaata accagacgcg
     6481 tgcggcaccc aacaccagta ctgcaaggat gtaaaccggg atcgtactcc agaaaagtgt
     6541 ccatagcgcc ctgccccagc ggctaaaaaa accgctctgt gcttccggta tgtcaatttc
     6601 gaccggtgcc tgcgtttgcg gtgtttcacg cacccatttt tgcaccagcg tcgcaatcag
     6661 caacgccatc accagcccgg ccaccagacg aatcgccgca aaaccccagc cgaggacaaa
     6721 ccccataaac accagcgtcg ccgggtttaa caccggattg cccatccaga atgccagcgc
     6781 accgcccatc gatacctgtt ggcgacgcat tcccgccgcg accggagccg cacagcaggt
     6841 acacatcatg cccggcagcg aaaacagcgt tcccagcagc gtgccgcgaa agcgcgattg
     6901 cccaagcgta cgcaacaacc agtcacgcgg gatcagcacc tgaatcaacg aaccgaggat
     6961 cacccccagc accgccgctt tccatactgc gaggaaatag atcatcgcgt aatccaacgc
     7021 cgcttgccac ggattagcat ccgcctgcgc aaggatagat ttaccgatac tgtgggtttc
     7081 ggctgcagta aacgctttgc cgtagtaagg ttgccatttc acataccaga gaccggcaat
     7141 gacaacgaga aagaaaagcg cgggtttcca ccactgaatg ggcgttgccg cctgagatga
     7201 agactgacca gtcatagcat tccccaggaa taagttatga taattgagcg cgtgaatatt
     7261 acgctcacta tcaattcttg ggaataatta ttcagctctt ttgcgtaatt ctgaagagct
     7321 taaaatcgtc acgccttcat gctcaggccg catcgcctct gccagcatga cccgcgccac
     7381 gtctctcgca tcaatggatt tccagttacc tggtaacaaa cggaacagcg gcgcaaaaag
     7441 cgtttcgttc atccgctgtt tgctacgatc gcccagtaac atcgacgggc gagcaatggt
     7501 caatttcggc cagttctggg caattaatgc ttcttccatc tcccctttga cgcggttata
     7561 gaaaaacggc gagtgggcat tggcacccat agcactgacc accagcatat gctgcgcgcc
     7621 cagtcgccgc ccggttaatg cggtatccac taccagcgtg taatcggcat gaataaacgc
     7681 ctctttgctc cccgcttctc gccgcgtggt gccgagacaa caaaacacaa tgtcgatggg
     7741 ttcggtgacc tgcgccaacg cgtcactcag ttgcggatca tggggattaa acaccccggg
     7801 catatcgccc aacggacgtc gcgtcggcgc agcaatggcg ttaactttcg gttcattaat
     7861 caacatccgc agcaggtgac cgcccaccag ccccgttgcg cctgtaatca gtacctgact
     7921 catcttcgct cctttacaga attgtctgcc ttgcgctccc cggctcatgc accagtctta
     7981 atagaccatg aggtaattat ccccgattgt ggaaaattcg cctcatccaa tgcaacaacg
     8041 cggaggaagc atgagtaaga aaattgccgt tttaatcact gatgaatttg aagattcaga
     8101 atttacttca cccgcagacg agttccgtaa agccggacac gaagtgatta ctattgaaaa
     8161 acaagcgggt aaaacggtga aaggcaaaaa aggagaagcc agcgtgacca tcgataaatc
     8221 catcgatgaa gtgacgccag cggagtttga tgccctgttg ctaccgggtg gtcattcacc
     8281 ggattatctg cgtggcgaca accgttttgt cacctttacc cgtgattttg tgaatagcgg
     8341 caaacctgtt ttcgccatct gtcacggtcc gcagctactg atcagcgccg atgtgattcg
     8401 ggggcgcaaa ctgaccgcag ttaaaccgat cattattgat gttaaaaatg cgggcgtgga
     8461 attttacgat caggaagtag tggttgataa agatcagctg gttaccagcc ggacaccgga
     8521 cgatctgcca gcgtttaacc gcgaggcgtt acgcctgctc ggtgcctgag tcgcgtagcc
     8581 aaatcatttt tttaccaaag cccagcgtgt tgtcggtgaa tttgatttca tcgagccgga
     8641 tttcccacac cggggccgac agcattctgg caaccggaaa gcgacgattg tacgccttgc
     8701 gcgcgaggtc gctttcctca ccttccagcc tgcggatctc acctttaaac tgcacaccgc
     8761 gaattaacgc taccgttttc ggctgaccgt ttaccgttcc ggcaaccgca gcctgcggcc
     8821 cactcatctg cgcgtggcgc gttttttctt ccgtcaaaat gtagaaggcg actttctgcg
     8881 catcaaaaag ataaaaggca ttagcgcacc agagttcccc ttcctgctgc acacaccagg
     8941 tgacaacatg ttgttttgcc agccaacggc tgatggcgat gagtgtttcc attgctgttc
     9001 tcttttatac tgtgggcctg accttaacat gcgatgcaga cgatgacacc ctggtttctt
     9061 tacttgatcc gtaccgccga caataagctt tataccggga tcaccacgga tgtcgaacgc
     9121 cgctatcagc agcaccaaag cggcaaaggg gcgaaagcac tgcgcgggaa aggagaactg
     9181 acgctggcgt tttctgcgcc agtgggcgac cgttcgctgg cactacgggc ggaatatcgc
     9241 gttaagcaac tgaccaaacg gcaaaaagag cgtctggtag cggaaggcgc agggtttgca
     9301 gaactgttga gcagcctgca aaccccggag attaaaagcg attgaaatgc tcgtgatact
     9361 caaccagacc ggttacgcca ttcagcgcgt catccgctaa acgatgtacc aggaaggcgc
     9421 tttcagtgcc cggccagcgg caacgcagat cgtgatgcgc cgccagttca aagccgaaac
     9481 gactgtacag cgccggatcg cccagcgtca ccactgcggc atagccgaac tcattaagcg
     9541 aatcgagtcc ttcatagacc agttggcgtg ccagcccctg tccacggtat ttttcatcaa
     9601 ccgccaacgg tgccatgccg acccattgca ggtcttcgcc ctgcacatca accggactaa
     9661 atgccacata gccaatgacc tgaccttcgt catctgtcgc caccagcccc agcgtcagaa
     9721 agccatcttc acgcagatcg tgaaccagct tcgcttccgc atcactttcg aatgagcgac
     9781 gcagcagggc atcaataccc ggcgcatcaa tgggaatttc tactcgaatt agcatggttc
     9841 acctaccgat gtctgtttgg tttcaggcgc ggttttcatt cccgcctcaa caaaatccgc
     9901 cagttgcagc agcatcatgc gtaaggcttt cggcatttgc tccagttcaa tggcgtccat
     9961 caggtttttc acatacaccc ccagctccgt atcgccttca atcaccagcc gacgctggaa
    10021 gaagagcgta tccggatctt gtttacgcgc cgcaatcatc agcagatcgc tggcgtcggc
    10081 actaaaactc acatcagctt gcgtgttctg gctgaccacc agtttgccat tcaccaccga
    10141 ggtaaaccat tgcaggtcaa tatcacgcac atgaatactt aaccagcggc cttcaagaaa
    10201 ctccagctcg ccatcatcca gcgcctgacg gaattgccag cttaagacct gctcaagaac
    10261 ctggcgtttt agcgcaaatg gcgtcagttt taccggtaca ctcaacagag atggccccaa
    10321 atgcacaata cgggaacgca gtttatccaa cacgagtttt actccctgtt tcaacaatca
    10381 tcctattttg ccatatcaga aaaataacat agcggtataa atcaacaatt ccatatgaaa
    10441 ttgctgctac caccaataca actttaactg ccttaaatca aaaattgtcg cagcaaggtt
    10501 aactaaaatc ccagttcgtt aacatttttg cgttttgata gcgcaacctt caggaaaaat
    10561 tatggagctg ctctgccctg ccggaaatct cccggcgctt aaggcggcca tcgaaaacgg
    10621 cgcagatgct gtttatatcg ggctaaaaga tgataccaat gcccgtcact tcgccggcct
    10681 taactttacc gagaaaaaat tgcaggaagc ggtgagtttt gtccatcaac atcgccgcaa
    10741 acttcacatc gcgattaaca cttttgcgca tccggacggt tacgcccgtt ggcagcgtgc
    10801 cgtggatatg gcggcgcagc tgggtgccga cgcgctgatc ctcgccgacc tcgccatgct
    10861 ggagtacgcc gccgagcgtt acccgcatat tgaacgtcat gtgtcagtgc aggcttcggc
    10921 gaccaatgaa gaggcgatta acttttatca tcgccatttt gacgttgccc gcgtggtgct
    10981 gccgcgcgtg ttgtcgattc atcaggtgaa acagctggca cgggttactc ctgtcccgct
    11041 ggaagtgttt gctttcggca gcctgtgcat tatgtcggaa ggccgttgct atctgtcgtc
    11101 gtatctgacg ggtgagtcgc ccaacactgt gggtgcgtgt tctccggccc gtttcgtgcg
    11161 ctggcaacaa acgccgcagg ggctggaatc ccgcctgaac gaagtgctga tcgaccgtta
    11221 tcaggacggc gaaaacgcag gttatccgac gctgtgtaaa gggcgttatc tggtggacgg
    11281 cgagcgctat cacgcgctgg aagaaccaac cagtctcaat accctggaac tgctgccgga
    11341 gttaatggct gcgaatattg cttcggtgaa aattgaaggc cgccagcgta gcccggcgta
    11401 tgtcagccag gtggcgaaag tctggcgtca ggctatcgac cgttgtaagg ccgatccgca
    11461 aaacttcgta ccgcaaagcg cgtggatgga gacgctcggg tcgatgtccg aaggcacgca
    11521 aaccaccctt ggcgcgtatc accgtaaatg gcagtgagaa gagcaatgaa atattcctta
    11581 gggccagtgc tgtggtactg gccaaaagag acgctggaag atttttatca gcaagccgcc
    11641 gccagcagcg ccgacgtgat ttatcttggt gaagcggtat gcagcaagcg ccgggcaacc
    11701 aaagttggcg actgtctgga gatggcaaaa tcgctcgccg gaagtggcaa gcagattgtg
    11761 ctctccacgc tggcgctggt gcaggcatcc tctgaactgg gcgaactgaa acgctatgtt
    11821 gagaacggtg aatttctgat tgaagccagc gatctcggcg tggtgaatat gtgcgctgaa
    11881 cgcaaactgc cgttcgtcgc cgggcacgcg ctgaactgct acaacgcggt gacgctgaaa
    11941 atattgctca aacagggcat gatgcgctgg tgtatgccgg tggagctttc ccgcgactgg
    12001 ctggtgaatc tgcttaatca gtgcgatgag ctgggcattc gcaaccagtt tgaagtggaa
    12061 gtcctgagct acggtcatct accgctggcc tactccgccc gctgctttac cgcgcgttcg
    12121 gaagaccgcc cgaaagatga gtgtgaaacc tgctgcatta agtatccgaa cgggcgcaac
    12181 gtgctgtcgc aggaaaacca acaagtgttt gtactcaatg gcattcagac catgagcggc
    12241 tacgtttaca acctcggtaa cgagctggca tccatgcagg gattggttga tgtagtccgc
    12301 ctgtcaccgc agggcactga cactttcgcg atgctcgacg ccttccgcgc taatgaaaat
    12361 ggcgctgtgc cactgccgtt gacggcaaac agcgactgta acggctactg gcggcggctg
    12421 gcaggactgg agctgcaagc ctaagtaaat agctcacttt gttaacaact ttaactattc
    12481 tttaatgcag tattaaagat taatcggtaa caaagtgagc tgttatgact gataaaacca
    12541 ttgcgttttc gctactcgat ctggccccca tacccgaagg ctcttcagcg cgggaagcgt
    12601 tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg cggctatcat cgctactggc
    12661 tggcagaaca ccacaatatg accggcattg ccagtgctgc aacatcggta ttgattggct
    12721 atctggcggc gaataccacc acgctgcatc tggggtctgg cggcgtgatg ttgcctaacc
    12781 acttaccgtt ggtcattgcc gaacagttcg gcacactcaa tacgctctat ccggggcgaa
    12841 tcgatttggg gctgggtcgt gctccgggta gtgaccaacg gacaatgatg gcgctacgtc
    12901 gtcatatgag cggcgatatt gataatttcc cccgcgatgt cgcggagctg gtggactggt
    12961 ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc aggctatggc gagaaaatcc
    13021 ccgtgtggtt gttaggctcc agcctttaca gtgcacaact ggcggcgcag cttggtctgc
    13081 catttgcgtt tgcctcacac ttcgcgccgg atatgttgtt ccaggcgctg catctttatc
    13141 gcagtaactt caaaccgtcg gctcggctgg aaaaaccata cgcgatggtg tgcatcaata
    13201 ttatcgccgc cgacagcaac cgcgacgctg aatttctgtt tacctcaatg cagcaagcct
    13261 ttgtgaagct gcgccgtggc gaaaccgggc aactgccgcc gccgattcaa aatatggatc
    13321 agttctggtc accgtctgag cagtatgacg tgcagcaggc gctgagtatg tcgttggtag
    13381 gcgataaagc gaaagtgcgt catggtttgc agtcgatcct gcgcgaaacc gacgctgatg
    13441 agattatggt taacgggcag attttcgacc accaggcgcg cctgcattcg tttgagctgg
    13501 cgatggatgt taaggaagag ttgttgggat agtgtgtctt aacgcgggaa gccttatccg
    13561 agctggcaac gctgtcctac gtaggcctga taagcgaagc gcatcaggca ttgtgtaggc
    13621 agcagaaatg tcggataagg caccgctgat tactgataca ccggcagtaa attaaagctc
    13681 gataaaatat gcaccagtgc gttgccgacg ccaaacacca gaatcagcgc aatcatcggc
    13741 ttgccacccc agacgcggaa tttcgggctg ccaaagcgtt tacgcgatgc gcgggccagc
    13801 agtgccggaa caattgccgc ccagatggta gccgctaaac cagcataacc aatggcatac
    13861 aggaatccgt tcgggaacag cagaccaccc acaacaggcg gggcaaaggt cagcaatgcc
    13921 gttttcaagc ggcccacagc cgagtcgtcg aaaccaaaca gatctgccag atagtcaaac
    13981 aaacccagcg ttacgccaag gaacgaactc gctaccgcaa agtttgagaa cacgaccagc
    14041 agaaggtcca ggctacggct gttcagcacg ccgcttaatg cttgtaccaa cacatcaata
    14101 ttaccgccct tctcggcgat gccgataaac tccggacgag ggatgttgcc cattgtcgcc
    14161 agcaaccaga tggtatacag cgccagtgcc atcagcgtac cgtacaccag gcatttcacg
    14221 atggttttcg gatctttgcc gtaatacttc atcaggcttg gcacgttacc gtgataacca
    14281 aacgacgcca ggcagaacgg cagggtcatc agcaaatacg gcgcataaga tgcattgctt
    14341 tcggcaacgt tgaacaatgt cgcaggctgc acatgcccca gcaggctacc gaaggtgagg
    14401 aagaaggtaa tgactttcgc ccccagcaca atcgccgtca tgcggctgac ggctttagta
    14461 ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa aacccgccgc ccgtgccggg
    14521 acgtttagcg acatctctgc gaaggtgtga tgcagaatcg aaccactggc agaaatatag
    14581 gcataggtca ggatatagag cacaaaggca atggaaatgc cgttgaccac gttccagcct
    14641 ttgcccagca aatctttggt gatggtgtca aaactcgaac cgattcggta attcaggtta
    14701 gcttccagaa tcatcaagcc ggaatgcagc atacagaacc aggtaaagat cagcgctgcc
    14761 attgaccaga aaaaccacgc cccggacatg accactggca gagaaaacat ccctgcgcca
    14821 ataatggtgc cgccgataat caccacgccg ccaagcagcg acggtgacgt ttgggtggtg
    14881 gttagtgttg ccatgagggc ttctctccag tgaaaaatag tgcgactgcg ttgttatgca
    14941 ttgcactgta ccagtacacg agtacaaaag acagaaaaaa agccccgatg gttaaaatcg
    15001 gggctgtata tattatttta cagattgtgt tcgctgttca gcgatgatta cgcatcacca
    15061 ccgaaacgac gacgaccggt agagtcatca cgacgcggtg cgcggccttc acgacgttcg
    15121 ccgctaaaac gacgaccatc accacgacca ccttcacggc gttcaccgct gaagttacga
    15181 ccgccttcac gacgctcgcc accgaaacca cgaccaccgc cacgacgctc accgccagta
    15241 tgcggctgtg catcgcccag taactgcatg ttcatcggct tgttgagaat gcgagtgcgc
    15301 gtaaagtgtt gcagcacttc acccggcata cctttcggca gttcgatggt ggagtgagaa
    15361 gcaaacagct tgatgttacc gatgtaacgg ctgctgatgt cgccttcgtt agcaatcgcg
    15421 ccaacgatat gacgaacttc aacaccatca tcgcggccca cttcaatgcg gtacagctgc
    15481 atatcgccaa catcacgacg ttcacgacgc ggacgatctt cacggtcgcc acgcgggcca
    15541 cggtcgttac gatcgcgcgg accacggtca tcacggtcac ggaattcacg tttcggacgc
    15601 atcggcgcat ctggcggaac aatcagagta cgttcaccct gtgccatttt cagcagtgcc
    15661 gcagccagag tttcgagatc cagctcttca ccttctgcag tcggctgaat tttgctcagc
    15721 agtgcgcggt attgatccag atcgctgctt tccagctgct gctgtacttt agcggcgaat
    15781 ttttccagac ggcgtttgcc cagcagttct gcgttcggca gttctacttc cggaatagtc
    15841 agcttcatag tacgttcaat gttgcgcagc agacgacgct cgcggttctc aacgaacagc
    15901 agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga tacggtgaac gtaagactca
    15961 gaatccatcg ggatatcgta gttaaccacc aggctgatac gctcaacgtc caggccacgg
    16021 gccgcaacgt cggtcgcaat caggatgtcc agacgaccgt ctttaaggcg ttccagtgtc
    16081 tgctcacgca gcgcctggtt catgtcaccg ttcagcgcgg cgctattgta gccgttacgc
    16141 tcaagagctt cagccacttc cagagtcgcg tttttggtac gaacgaagat aatcgccgca
    16201 tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt ttttacgcat accccacaca
    16261 gtccagtagc tctggctgat gtcaggacgg gtagtcacgc tggactgaat acgcacttcc
    16321 tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg cttccggcat ggttgcagag
    16381 aacagagcgg tctgatgacc ttccgggatc tgcgccataa tggtttcaac gtcttcgatg
    16441 aagcccatgc gcagcatttc gtcagcttca tccagaacca gaccgctcag tttagagagg
    16501 tccagagtgc cacgtttcag gtggtccagc agacggcccg gagtaccgac aacgatctgc
    16561 ggcccctgac gcagggcgcg taattgcacg tcataacgct ggccgccgta cagggcaacc
    16621 acatttacgc cgcgcatgtg tttagagaaa tccgtcattg cttcagcaac ctgtaccgcc
    16681 agttcgcggg tcggtgccag caccagaatc tgtggtgctt tcagctcagg atcaagattc
    16741 tgcagcagag gtaaagagaa tgctgcagtt tttccgctcc ccgtctgggc catacccaga
    16801 acgtcgcggc cattcagcag atgtggaata cactctgcct gaattggaga tggtttttcg
    16861 taacccagat cgttaagggc ttcaaggata ggagccttca ggcccagatc tgcaaaagtg
    16921 gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta atggcggcca gtctacataa
    16981 ctcatcatga aattgatcag caattttcat tgaaaagtgt gaaccggctc aaagtaggtg
    17041 tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg caatcaaaaa agattacggg
    17101 ctgatgtgta cgtcagctat tgctggtccg attctgccag gtcatcttgg tcctggccca
    17161 ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa gttatgaacg ttgttggcaa
    17221 ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc cagacttagg tagtacttac
    17281 ctaaatagaa gttggtttca ctgagatgct cagcgagcga ggtgttatcc gttgcgtccg
    17341 ccttgagcct ttccattaac gtttgttcgc taatgttgcc caggtagaac tcgacaatgt
    17401 tccatcccca ctgttcctta tccgattttt cgaagtgctg tttcaatact tctttagcct
    17461 gcttctcatc gagcttctgc tcggcgagat aaagccacag actacggaaa ggatcattgg
    17521 gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa cttgtcacga ccgccgtaat
    17581 ataatgcaat cccgcgattc aagtgcgcgt agttgtaagt tggatcaagc tcaagtacag
    17641 aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg cgttaaatat atgcctaagt
    17701 aattgaatac ttcaggcata tccggtcgga ttgccagcgc ttgcgaaaaa tcgttacgcg
    17761 ctaatgccct cagaccgaga ctatcataca acactccgcg ctcatataaa agctgtgcgc
    17821 gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc catacgtgcc agaatcactt
    17881 cctgctgtaa agtcggttgc aatggtaccg cgaggacttc acttttacgc caggaagtat
    17941 tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca ccagcgcaaa aaaggcttca
    18001 tttcccactc ccgaagacca cggttgaatg aacgtcctgt tcccggttgc taacaaggcg
    18061 tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa atggcaacct tactcgccct
    18121 gttcagcagc cggagcttcc ggtgctgcag caggttgaga ctgctcagtc gcttctttaa
    18181 tgctcagacg gatacggccc tggcgatcaa cttccagaac tttcaccggt acttcctgac
    18241 ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc agcgatttga gagatgtgta
    18301 ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc aaagtcaacg atacgggtca
    18361 ctttaccagt gtagacgcgg cccacttcga tttctgcagt gatctcttcg atacgacgaa
    18421 tagcatgttt cgctttctcg ccgtcggtcg ctgcaatctt cacagtaccg tcatcttcga
    18481 tttcgatggt ggtgccagtt tcttcggtca gggcacggat aacagagccg cctttaccga
    18541 taacgtcttt gatcttgtcc gggttgatct tgatggtatg gatacgtggt gcgaactcag
    18601 agatatcgcc acgcggcgcg ttgatcgcct gttccattac gcccaggata tgcagacgcg
    18661 cacctttagc ctggttcagc gcaacctgca tgatctcttt ggtgatacct tcaattttga
    18721 tatccatctg caatgcagag ataccctcgc gggaacccgc aactttgaag tccatatcgc
    18781 ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac gtagttgtcg ccttctttca
    18841 ccagacccat tgcgataccc gcaacggcag ctttgatcgg cacacctgcg tccatcagcg
    18901 ccagagacgc gccgcacacg gaagccatag aagaggaacc gttggattca gtgatttcag
    18961 acacaacacg tacggtgtac gggaatttgt ccatatccgg catgactgcc agcacgccgc
    19021 gcttcgccag acgaccgtga ccaatttcac gacgcttcgg agaaccgacc atgccggttt
    19081 cgcctacgga gtacggaggg aagttgtagt ggaacaggaa ggtatcggta cgttcgccca
    19141 tcagttcatc aagaacctgc gcgtcacgag cagtacccag cgttgcggta accagcgcct
    19201 gcgtttcacc acgggtgaac agcgcagaac cgtgagtacg cggcagcacg ccagtacgca
    19261 catccagacc acggatcata tctttttcac gaccgtcgat acgcggttcg cctgccagta
    19321 cgcggctacg aacaacgttt ttctcgatcg cgtgcagaat ttcacccagt tcgttttcgt
    19381 ccagggtttc gtcttcagca agcagcgtcg cgatggtttc agatttgatg acatcaacct
    19441 gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc gctcaggcga gcttcagcca
    19501 gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc cggctgccag tcccaacgtg
    19561 gtttaccggc ttctttcacc agttcattga tgttctgaat aacaacctgc tgttgttcat
    19621 gaccgaacac tactgcgccc agcatctggt cttcgctcag cagttcagct tcagattcaa
    19681 ccatcagtac agcggcttca gtaccggcaa caaccagatc cagtttgctc tctttcaact
    19741 cgtcctgagt cgggttcagt acgtactggt cattgatgta acctacgcga gcagcaccaa
    19801 tcggaccatt gaacggaata ccagacagag acagtgctgc ggaagcacca atcatcgcga
    19861 cgatatccgg gttaacttgc gggttaacag aaaccacggt ggcgataacc tgaacttcgt
    19921 tgacgaagcc ttccgggaac agcggccgaa tcgggcggtc aatcagacgc gcgatcaggg
    19981 tttcgccttc gcttgggcgg ccttcacgac ggaagaagct acccgggata cgaccagcag
    20041 cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa gtcctgacct ggtttggctt
    20101 ttttctggcc aacaacggta acaaataccg cggtgtcatc catgctaacc ataacagcgg
    20161 cagtagcctg acgagccatc atgccggttt ccagagtcac ggtgtgttgg ccgtactgga
    20221 atttacgaac gatcggatta agcaatgtaa tatcctttct ctttcttaga cagtacctta
    20281 cggcactggt gttaataccc gatcttctgc gcatcctcgc gactaatgac aaccctaacc
    20341 cagctctatg tgggtaaagc ctctcattag ccgcgcgaac ctctgcaacg gaagatcatt
    20401 catagcaaca atacattagt ttccagtgaa ttgctgccgt cagcttgaaa aaaggggcca
    20461 ctcaggcccc cttttctgaa actcgcaaga attagcgacg cagacccagg cgctcgatga
    20521 gctgggtgta acgtgctacg tctttacgtt tcaggtagtc gagcagttta cgacgctgag
    20581 aaaccatgcg cagcagacca cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc
    20641 cctgcaggtg gttgatctgt gcagtcagca gtgctacctg aacttcagta gaaccggtgt
    20701 cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt tgcttcagta cttagagaca
    20761 ttttaaaact ccaaagtata tagaatgaaa ggacgccgat ctctaattca gcgatcccag
    20821 tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta agcggcagta ttctactcgt
    20881 agcgacctgt tatcgcaaga cggttaacat tacgccgggt attcaaccac caggcgacga
    20941 ggcgcaacgc ggccttcatc gtcaatttcg cccataccga taaatttgcc gttctcacct
    21001 tctgtaacgc gaaccagtcc ttccagcggc gcaccggatg tacgaactgg gttaccattt
    21061 ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg ggtagtccga agctggactg
    21121 tccattggca tcagtaatgg atcaagtaac tccgcagccg gaatatcctg ctgttcggct
    21181 tgttcgacca gttcacgcag atgttccagg gtcacaatcc gttcaaccgg atatttactt
    21241 accgccagac gacgcaggta aataacatgc gcgccacaac cgagtttttc acccaggtca
    21301 tcaatgatgg tgcggatata agtgcctttt gagcagtgaa tttccagctc cagctcattg
    21361 ccttcgtggc gaataaacag caattcataa acggtaatcg ggcgcgcttc acgcggtact
    21421 tcaatgccct gacgcgcata ttcgtacagt tttttaccct gatatttcag tgctgaatac
    21481 atcgaaggga tctgttcgat atcgccacgg aaagtatcca gcgccgccgc cagttgctct
    21541 gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt cggcatcaga agtatcggta
    21601 cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg agtccagcag atactgggaa
    21661 aacttcgtcg cttccccgag gcaaattggc aacatgccag ttgccagcgg gtccagcgca
    21721 ccggtatgcc cggcacggtt ggcgttataa atccgtttca ctttttgcag cgcatcattg
    21781 ctggacatac cctgcggttt atccagcaac aaaacgccgt taatgtcgcg accgcgacga
    21841 cgaggacgac tcattagtcc tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat
    21901 gtttgaccac gctggtcacc aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt
    21961 agaagaaggt cagttccggc acgatacgca ggcgcatcgc tttccccagc aggctgcgga
    22021 tgaaaccaga agcttcttgc aacgctttga tgcccgcttt aaccgcgtct tcatctttgt
    22081 cgttgaggaa cgtcacatat actttggcat acgccaggtc gcgagacatt tcgacaccgg
    22141 aaacggtggt catcatgccc aggcgaggat ctttaatttc acgctgcagg atgagagcga
    22201 tctctttttg catttcctgc gctacgcgct gcgggcgacc aaattctttc gccataataa
    22261 attctcctga caaaaaaggg gctgttagcc cctttttaaa attaatttca ggtggaaggg
    22321 ctgttcacgt tggcctgata agacgcgcca gcgtcacatc aggcaatcca tgccggatgc
    22381 ggcgtaaacg ccttatcccg catggaaccc taaaaacctt aagcgatggt acgctggatc
    22441 tcgatgattt cgaatacttc gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg
    22501 ccgataccac attccatacc gttacggact tcgttaacgt catctttgaa gcggcgcagg
    22561 gactccagct cgccttcgta gataaccacg ttgtcacgca gaacgcggat cgggttgtga
    22621 cgtttaacca caccttcggt aaccatacag cctgcgatgg caccaaattt cggcgatttg
    22681 aacacgtcac gaacttccgc cagaccgata atctgctgtt tcagttccgg agacagcata
    22741 ccgctcatcg ccgctttcac ttcgtcaatc aggttataga tgacggagta gtaacgcaga
    22801 tccaggcttt ccgcttcaat cactttacgt gcagaggcat cagcacgtac gttgaagcca
    22861 accaggatgg cgttggacgc cgcagccagg gtggcgtcgg tttcggtgat accacctacg
    22921 ccagaaccga tgatcttcac tttaacttcg tcagtagaca gtttcagcaa ggagtcggag
    22981 atcgcttcca cagaaccctg tacgtctgcc ttcaggacga tgttcacttc gtgaacttcg
    23041 ccttcggtca tgttggcgaa catgttctcg agtttagatt tctgctgacg cgccagttta
    23101 acttcgcgga atttaccctg acgatacagt gcaacttcac gcgctttctt ctcgtcacgt
    23161 acaacggtaa cttcatcacc cgcagccggt acgccggaca ggccgaggat ttccaccgga
    23221 atggacggac ccgcttccag cacttcctga cccagttcgt tacgcatcgc acgaacacga
    23281 ccgtattcga agccacacag aacgatatcg cccttgtgca gagtaccttc acgtaccaga
    23341 acggtagcaa ccggaccacg acctttatcg aggaaggatt cgataaccgc accgctcgcc
    23401 atacctttac gtaccgcttt cagctccaga acttccgcct gcagcaggat agcgtccagc
    23461 agttcatcga taccagtacc cgctttcgca gacacatgta cgaactggct ttcaccgccc
    23521 cactcttccg gcaggatgcc gtactgggag agttcgttct taacgcgatc cggatcggct
    23581 tctggtttat cgatcttgtt cactgcaacg accaccggaa cctgcgccgc tttcgcgtgc
    23641 tggattgctt cgatcgtctg cggcattacg ccgtcgtcgg cagcaacaac caggactacg
    23701 atgtccgttg cctgcgcacc acgagcacgc attgaagtaa acgcggcgtg ccccggggtg
    23761 tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt atgcaccaat gtgctgggta
    23821 atgccgcccg cttcgccaga ggccactttc gttgaacgaa tgtagtccag cagagaggtt
    23881 ttaccgtggt caacgtgacc catgatggtc acaaccggcg cgcgcggttc agccgcagca
    23941 cccgtgtcac ggtcgctcat taccgcctct tccagctcgt tttcacgacg caggataact
    24001 ttatggccca tctcttcagc aaccagctgt gcggtttcct gatcgataac ctggttgatg
    24061 gttgccattg cgcccagttt catcatcgct ttgatgacct gagagccttt aaccgccatc
    24121 ttgttcgcca gttcgccaac ggtgatagtt tcgccgatca caacgtcacg gttaacggcc
    24181 tgagcaggct tctggaagcc ttgctgcagc gaagaacctt tacgttttcc gcctttaccg
    24241 ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg attcagcgtg tttgttgcct
    24301 ttcttcggac gcgctgcttt cgcgttacga ccacggccac ggccgccttc gacttcacga
    24361 tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag tgacgtgata atcgctggaa
    24421 tcttcagtcg gttccgcgtt atcagtccat ttgttttctt ccgccatacg acgtgcttct
    24481 tcagcaacgc gacgtgcttc ttcttcgagt ttacgacgcg cttcttcttc agctttacgc
    24541 ttgagctctg cagcttcctg ctcacggcgg gctttttcag cctgggcgtt tttagtcata
    24601 tcgtcttgtt gattgctcac tttgtctttt tccgcagctt cacgtttcgc ttgttcagca
    24661 gcttcacgct tagcttgttc tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta
    24721 gccgattctt ctgcctcacg acgggcttgc tcttccgctg caaggcgttc agcctcttgc
    24781 ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga tttgtaccga tttgcttttt
    24841 ccaccggtac caggaatgtt aagggtgctg cgtgttttac gttgcagcgt caatttgtcc
    24901 gggcctgaat ttttctgatt caggtggtca atcaaagtct gtttctcttg tgcagacaca
    24961 gagtcgtcag cagacttccg gatacctgca tcagcaaatt gctgtaccag gcgttccacg
    25021 gaggtctgtc gctctgcggc cagcgtttta atcgttacat ctgtcatgct gttccttcct
    25081 gctacagttt attacgcttc gtcaccgaac cagcaaatat tacgggcagc cataatcagt
    25141 gctccggctt tttcgtcggt caacccttcg atatcagcca gatcatcaat gccctgttcg
    25201 gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa atcacgatct
    25261 accccttcaa ggttcagcag atcgtcagcc ggtttgttat caccgaggct ttcttcctgg
    25321 gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc aacggtcggc
    25381 tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccag ttcttccagc
    25441 gtcgagaaac cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg
    25501 gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg
    25561 gtcattacgt tgagttccca accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta
    25621 cggccaatcg cctgcgccag gttaccagct tcaacggcga tatccatggt gtgtttatct
    25681 tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac aaactgcgcc
    25741 gggttatcat cccacaggac gatatcgata cgctcgccgc ccagttcagt agaaaccgcc
    25801 tgaacacgcg cgccacgcat acctacgcaa gcacctaccg gatcgatacg tttatcgttg
    25861 gttttcaccg cgattttcgc acgagaaccc gggtcgcgag ccgctgcttt aatttcaatc
    25921 acttcttcgc cgatttccgg cacttcaata cggaacagtt cgatcagcat ttccggcttg
    25981 gaacgagtga cgaacagttg cgcgccacgc gcttccgggc gaacggaata aagcacgcca
    26041 cgaacgcggt cgccagggcg gaagttttca cgcggcagca tatcttcgcg caggatcacg
    26101 gcttcagcgt tgttgcccag atccagagag atgttgtcgc ggtttacttt tttcaccacg
    26161 ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc acgttcggct
    26221 tcacgcactt tctgcacgat aacctgtttt gccgtctggg tggtgatacg gtcaaaggta
    26281 acagactcaa tctgatcttc aacgtaatcg cccaagttca ggctttcatc ttcataacgt
    26341 gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac taaccagcga
    26401 cggaaggtgt caaaatcacc gcttttgcga tcgatctgta cacggacgtc gatctcttgc
    26461 ccatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg
    26521 cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc tttgttcatc
    26581 gcgggctttt cacctcatcc agactattaa aagtggggaa ccaggttcgc cttctggata
    26641 ttactcagcg cgaacacttc atctttacct tcgacggtaa ctgtgatcat ttcaccgtct
    26701 accgctttga taacgccctg ccatttacga cggttttgta ccgccatacg gagaaccaga
    26761 gtcacctctt ctccgacaaa acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga
    26821 cccggtgagg agacttccag gttataagca acggtgatgg gatcttcaac atccagcaca
    26881 gcacttacct ggtggctcac atcagcacaa tcatcaacat tgatgccatc ttcactatca
    26941 atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt cgatgcctac cagttcaaaa
    27001 cccagggcct caactggcgc agtaatcatc tctgttaatt tttgctctaa tgtggacaag
    27061 cccaccccca agacataaaa aaagggccta aagcccagtt attctgtagt cagataacaa
    27121 aaaaccccga taaatcgggg ctttatataa ctgaacccta taaccgcaac tgcggtctgg
    27181 agcactttcc agaaggattt tttcaaatcc cactacgaag gccgaagtct tcacagtata
    27241 tttgaaaaag gactctaagg gaaagtggtt gcgggggccg gatttgaacc gacgaccttc
    27301 gggttatgag cccgacgagc taccaggctg ctccaccccg cgcctgaaac gtggcaaatt
    27361 ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt tggtgtaccg agacgggacg
    27421 taaaatctgc aggcattata gtgatccatg ccacattttg tcaacgttta ttgctaatca
    27481 cgtgaatgaa tatccagttc actttcattt gttgaatact tttgccttcc cctgctctcc
    27541 cttaagcgca ttattttaca aaaaacacac taaactcttc ctgtctccga taaaagatga
    27601 ttaaatgaaa actcatttat tttgcataaa aattcagtga gagcggaaat ccaggctcat
    27661 catcagttaa ttaagcaggg tgttatttta tgacgacgat tctcaagcat ctcccggtag
    27721 gtcaacgtat tggtatcgct ttttccggcg gtctggacac cagtgccgca ctgctgtgga
    27781 tgcgacaaaa gggagcggtt ccttatgcat atactgcaaa cctgggccag ccagacgaag
    27841 aggattatga tgcgatccct cgtcgtgcca tggaatacgg cgcggagaac gcacgtctga
    27901 tcgactgccg caaacaactg gtggccgaag gtattgccgc tattcagtgt ggcgcatttc
    27961 ataacaccac cggcggcctg acctatttca acacgacgcc gctgggccgc gccgtgactg
    28021 gtaccatgct ggttgctgcg atgaaagaag atggcgtgaa tatctggggt gacggtagca
    28081 cctataaagg aaacgatatc gaacgtttct atcgttatgg tctgctgacc aatgctgaac
    28141 tgcagattta caaaccgtgg cttgatactg actttattga tgaactgggc ggccgtcatg
    28201 agatgtctga atttatgatt gcctgcggtt tcgactacaa aatgtctgtc gaaaaagcct
    28261 actccacaga ctccaacatg cttggtgcaa cgcatgaagc gaaggatctg gaatacctca
    28321 actccagcgt caaaatcgtc aacccgatta tgggcgtgaa attctgggat gagagcgtga
    28381 agatcccggc agaagaagtc acagtacgct ttgagcaagg tcatccggtg gcgctgaacg
    28441 gtaaaacctt tagcgacgac gtagaaatga tgctggaagc taaccgcatc ggcggtcgtc
    28501 acggcctggg catgagcgac cagattgaga accgtatcat cgaagcgaaa agccgtggta
    28561 tttacgaagc tccggggatg gcactgctgc acattgcgta tgaacgtctg ttgaccggta
    28621 ttcacaacga agacaccatt gagcagtatc acgcgcatgg tcgtcagttg ggccgtctgc
    28681 tgtaccaggg gcgttggttt gactcccagg cgctgatgct gcgtgactct ctgcaacgct
    28741 gggttgccag ccagatcact ggtgaagtta ccctggagct gcgccgtggg aacgattatt
    28801 caatcctgaa taccgtctca gagaacctga cctacaagcc agagcgtctg acgatggaaa
    28861 aaggcgactc ggtgttctcg ccagatgatc gtattggtca attgaccatg cgtaatctgg
    28921 atatcactga tacccgagag aaactttttg gttatgccaa aactggcctg ctttcctcct
    28981 ctgccacttc aggcctgccg caggtggaga atatggaaaa caaaggccag taattcgaca
    29041 tcaaccctgc ccttcagggg tggggttacc ttgcttatcc tctgtaaact cctttctgag
    29101 catcacataa tcgaaaacat catggtttgc atccacaacc cgtgagtctc ctttatagtg
    29161 tgcaatcact tcctcaagcg tttgcggctg ttccggcttt gttaccccca tccacgcatt
    29221 aataaggtaa ttattgtaag ctgtcgaaaa gatattgttt tccgttgtgc gatccacgcc
    29281 atccccaaga acggggctat accagataaa catcgggaca tgatatgcct gctggctagc
    29341 ctccctgcct ccatgaaaat agacgttctt cttcgtaggg tcacgttcca ggccgtggtc
    29401 ggcaaaatac atgaccgagg cgcggcgatc ttttaataat tcaaaaacct gacctagcaa
    29461 actatctgtg taatgaatgg agttgtcata gcaggcatcc tgatcgtcct gcggttgaaa
    29521 cacggcgctg gattgcggat aggcgctaca agccggttca tggcttccat ttaaatgaag
    29581 aacaatcagt ttcttttgct gcgtattttg ctgtaatgct tgcgataaat gcggcaacaa
    29641 caattcatca aatcctctga cataaactgt ttccatggcg cgcatggcga tactggtaac
    29701 tgctgtacca ttctgccgaa aagcggattg cgagcttagc cagaaagtct gaaatcctgc
    29761 ttgattagcc atattaataa tgttgtcggg gtaattatga atgtcatgac tcaaaacaga
    29821 atcagcagtt aaagaaaggg gaaccgacag cgcagtgtaa ggtgcgccgc ttattgcttg
    29881 attaaacagt ttgatctgtt ttctttgtgc ttcaacttgc ggtgtcgtag agcgtgtata
    29941 tccatacaaa gacatattgt cgacacgtac agactccccc acaatcaaca cgtaggtatc
    30001 aatacttgta tccctgactg ataattgaaa atacggcacc gtgtttgcaa ttgagagtaa
    30061 tctttgatgc tctttcgctg ctaaagcaaa atagttgaga ttgaaaaacg gcgtataggt
    30121 agcaaatcac gacgctagta tatatggact gaacgctttt ttattttttg catctttata
    30181 agcaaattga caagcggaaa ataaactgcc cgaaatgaca atcagcaata atattcctgt
    30241 cacttttttt gtcggcaagg aaacatcata ttttattatt actgccaaaa aaagaaggga
    30301 taaaaaagca aaagcacata gataagggat atacatcccc agcattttca caacttcatc
    30361 cggatcactc tgcagcacac taatcgcaaa tccatcattg aaagttgtac caaaggtaca
    30421 ccatgaatag aggctaatac tcatgtctgc cgcagtcagt aacacaaatg gtgcggcaac
    30481 aagcaggcga agcggaaggg atgatatacg tttaacaacc aggatggtca ggacgaagaa
    30541 cagcaatcgc tgaaccatat gcgctccgga agaggcgact aagttcgtaa taccaaaaac
    30601 aataacacaa agatacaaca accaatgaga tttaaaatct tctagcaaat ttgttgaata
    30661 ctgtcatcca gaaatccaag ttcacaacca atataaacaa ttatcaacaa tgttcaattt
    30721 tatatcagac atgtttttca actctgatcc atatcagacc gaaacactgt tagttttttc
    30781 tttttaaatg atgcgaccat tgcgttctca gtaattaaat aacattatca atgcgtctct
    30841 aaacattacg cagaacgtag ccatcagtag ttatactgac aggagcagga tggtttatcg
    30901 agaagtaaac atatccgtat tcgtaccgga actggagttg acacaataaa gtgccaatta
    30961 tgtcagcagt agggaaaagt ttataactaa ggcgtaccgg caccatcgtt tcaaggtacc
    31021 agctacgagt aaagcaactg gacgagatac agatacctga caaccattca tcagaccagg
    31081 accaaaacga aaagagacgc ttttcagcgt ctcttttctg gaatttggta ccgaggacgg
    31141 gacttgaacc cgtaagccct attgggcact accacctcaa ggtagcgtgt ctaccaattc
    31201 caccacctcg gcacggatac tactttttag ttcgggatat cgctggtcgg cttagccgga
    31261 gcagctggct gagtttgttc ggttttcgcc ggtgcactca gattttccca ttcgctacct
    31321 ctattggttt tgttgctatt gatgttaccc agcaccagac tgatgatgaa gaataacgtt
    31381 gccagcagcg ccgtcatgcg ggtcatgaag ttaccagaac cacttgaacc aaacagcgta
    31441 gcggaagcgc ctgctccgaa ggaggctccc atatcagcgc ctttaccttg ctgcagcatg
    31501 atcagaccaa caaggccaat tgccacaata aggaaaacta ctaaaagagc ttcgtacata
    31561 atcaacctgt tccttgcgga gttgccgcgt accaatgctt caaccaataa agcgggagtt
    31621 tttaatcttt cccactgaag cgggtgtgaa tactaaccaa aacgaatgac cttcgcaagg
    31681 gcaattttat cgcattgtat caactgcgga aaaaaacagc aaaaccgatg tgttcgccga
    31741 gaaaaaaggc gacaatcgcc gcctttttag ccagttatct aacgctttaa acggctttta
    31801 ctgcatcggc gatgcggtgt gcaaattcag tcacctgcgc ttcgtcttcg ccttccacca
    31861 tcacgcgaat taacggttcg gtgccggatt tacgcagcaa cacgcgtcca cggttgccca
    31921 gcgcagcttc aacctctgcg gtcacggctt taactgactc atgctcaagc ggatcgccgc
    31981 tacctgcggt gtaacgtacg ttaaccagaa tctgcgggaa cattttcatg ccgctgcaaa
    32041 ggtcgtgcag gctcatatgg ttacgtgcca tcgccgccag cacctgcaag ccagcaacga
    32101 tgccgtcacc ggtagtggtt ttatccagca ggatcacatg accggaattc tctgcaccga
    32161 tacgccagcc tttctcctgc attttttcca gtacgtagcg gtcacccact ttcgcgcgcg
    32221 caaatggaat tcctaactgt ttcagcgcca gttcaagccc catgttgctc atcaatgtac
    32281 ccacagcacc accacgcagc tggccctgac gaagaccttc acgcgcgatg atatacatga
    32341 tctgatcgcc atcgacttta ttgccttcat ggtcaaccat aatcacgcga tcgccgtcgc
    32401 cgtcgaaggc aataccgaga tccgcttttt cagccagcac acgagcctgg agcgcgcgaa
    32461 cgtcggtagc ccccacttcg gcattgatgt ttacaccgtt tggctcacaa ccgatagcga
    32521 taacgttcgc ccccagttcg cgcagcacgt tcggcgcgat gtgataagtc gcaccgtttg
    32581 cacaatccac cacaatcttc agttcactga ggctaagttc gttcgggaac gtggctttgc
    32641 aaaactcgat atagcgaccc gcggcatcaa cgatacggct ggctttaccc agttctgccg
    32701 aatcaacgca gctgatctcc ttttccattt ccgcttcgat ggcctcttct accgcatccg
    32761 gcagtttagt gccgtcgata gagaagaatt taatcccatt atcgtagaac gggttatgcg
    32821 atgcagatat cacaattccg gcctctgcgc ggaaggtacg cgtcagataa gccacggccg
    32881 gtgttggcat cgggccagtg aagagtgcgg aaaggcccgc tgccgccaga cccgcttcca
    32941 gtgctgactc cagcatatag ccagaaatac gcgtgtcttt accaataata atcttacggg
    33001 agccgtggcg cgccagcact ttacccgcgg cccaacccag cttaagcaca aaatcaggtg
    33061 tgatcggcgc atcccctaca cgaccacgaa tcccatcggt accgaaatat ttacgattac
    33121 tcatagcgtt tgttttcctt tgcagacaga gtggcttcca ccacccgcat cgcttctacg
    33181 gtttctttga cgtcatgaac acgaatgatg tgcgcgcctt gcattgcggc aatgaccgca
    33241 caggccagac taccgctcag gcgctcggac ggccccacgt tcagcagctg cccaatcatc
    33301 gattttcgtg acatacccac caacagcggc aggttgaaat ggtgaaattc agccaggcgc
    33361 gccagtaatg aatagttatg ggagagattt ttaccgaaac cgaatccggg gtcgagcaac
    33421 aatttctctt ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat aaagtagcga
    33481 ttcacttctg caaagacatc gtcatacttc ggagcttcct gcatggtttt tggatttccc
    33541 tgcatatgca tcagacaaac cggtaaaccg gtttctgcag ccgcctccag agcgccaggt
    33601 tcggaaaggg agcggatatc attaataatg tgagcgccaa ctttcgctga ctcacggatg
    33661 acttctggtt tggatgtatc gaccgagatc cagacttcga agcgttgagc aattgcctca
    33721 accacaggaa taacacgttg caactcttct tcaacgctaa cttccgccgc ccctgggcgc
    33781 gtggactcgc caccaacgtc aatgatcgtc gcgccagcat tgatcatcag attcgcatgt
    33841 ttcaccgcat ctatcagcga gttatgcgtg ccaccatccg aaaaggaatc aggcgtgacg
    33901 ttgaggatcc ccattacgtg aggatggcta aggtccagtg aagtaccctg ggcaaagagt
    33961 ttcattatgt tatccctggt atgaattgat aagaaaaaac cccggagcac gccccggggt
    34021 tttcggtaca aatacagtca tctgatgcgg gaacttactt gtcgcctaac tgctctgaca
    34081 tggtgttacc cgggttcggc gtacgcggtt catcaaccgg acgaggagcc tttggactac
    34141 cattgtcgcc agaattgtta gaagcgcctg gttcttccca gcccgctggc ggacgtacat
    34201 cgcgacgtgc catcaggtca tcaatctgcg gtgcgtcgat agtctcatat ttcatgagag
    34261 catctttcat cgcatgcaga atatccatat tgtcggtcag aagctgacgc gcacgattat
    34321 agttacgctc aatcagtgct ttcacttcct ggtcgatgat acgtgcagtt tcatcggaca
    34381 tatgtttcgc tttcgctacg ctacggccga ggaatacttc accttcttct tccgcgtaca
    34441 gcagtggacc caatttctca gagaagcccc actgagtcac catgttacgt gccaggttgg
    34501 tcgcaacttt aatatcgttg gacgcaccgg tagaaacatg ttccggcccg tagatgatct
    34561 cttctgccag acgaccaccg tacagcgtag aaatctggct ttccagtttc tgacggctgg
    34621 cgctgattgc gtcgccctca ggcaagaaga aagtcacacc cagcgcacga ccgcgtggga
    34681 taatcgtcac tttatgcacc ggatcgtgtt ccggcaccag gcgaccgata atcgcatgac
    34741 ccgcttcgtg gtaagccgtt gattctttct gcgcttccgt catcaccatg gagcgacgtt
    34801 ccgcacccat catgattttg tctttcgctt tctcgaactc aaccatcgac acaacgcgtt
    34861 tgttgccacg agcagcgaac agtgccgctt cgttcaccag gttcgccagg tcagcaccgg
    34921 agaaaccagg agtaccacgg gcaatgattg ccgcgtcgat atcgggtgcc aatggtacgc
    34981 gacgcatgtg aactttcagg atctgctcac gaccgcgaac atctggcaag ccgaccacaa
    35041 cctggcggtc gaaacggcca ggacgcagca gggccgggtc gagaacgtcc ggacggttag
    35101 tcgcggcgat aacgatgata ccttcgttac cttcgaagcc atccatctca accagcatct
    35161 ggttcagagt ctgttcacgt tcatcgtgac caccgcccag accagcgcca cgctggcggc
    35221 ctacggcgtc gatttcatcg ataaagatga tgcacggtgc cgctttcttc gcctgttcga
    35281 acatgtcacg aacacgggat gcacccacac cgacgaacat ttctacgaag tcagaaccgg
    35341 agatagtaaa gaacggaact ttcgcttcgc ctgcaatcgc tttcgccagc aacgttttac
    35401 cggtacccgg aggaccgacc atcaggacgc ctttcgggat cttaccgccg agtttctgga
    35461 agcggctcgg ctcgcgcaga tactctacca gttcagcaac ttcttctttt gcttcgtcgc
    35521 agcccgcaac gtcagcaaag gtcgttttga tctgatcttc cgtcagcatg cgcgctttgc
    35581 tcttaccaaa cgacatggca cctttgccac cgccgccctg catttgacgc atgaagaaga
    35641 tccagacacc aatcagcaac agcatcggga accaggagat gaagatagaa gccagcaggc
    35701 ttggttcttc aggcggttca ccgacaacct tgacgttctt ggtcaacagg ttatccagta
    35761 atttcggatc ctgaaccgga atgtaagtgg tataacggtt actatctttc ttggtaacgt
    35821 tgatttcacg tccgttgata cgcgcttcac gaacctggtc gttattgacc tcttgtagga
    35881 aggtagagta atccacctta cggccattag actcgctggg cccaaagctc tggaatactg
    35941 acatcagcac aacggcaatg accagccaga gtattaggtt tttcgccatg tcactcaagg
    36001 gattaacctc ttattacaac tgtgttaaaa acagcgtcag gatactctat atccagcatc
    36061 tttcaaactt tcgtctgaaa tctcccggtt agggtttacg cccggtcgct acaatataca
    36121 cttctcgcga acgtgcacga gaagagtccg gcttacgaac tttgaccttc gtaaacaggg
    36181 agcgaatttc ccttagatac tcatcgaaac cttcgccctg gaacaccttc actacaaaac
    36241 tgccacctgg cgctaataca tcacgacaca tttctagcgc cagttccacc agatacatgg
    36301 ctcgggggat atccaccgcc ggtgttccgc tcatgtttgg tgccatatcg gacatgacaa
    36361 cctggacttt gctgtcgcca acgcgctcca gcagtgcttt catcaccagt tcatcacgaa
    36421 aatcgccctg aagaaagtcc acaccaacga taggatccat aggtaaaaga tcgcaagcga
    36481 tgatgcggcc tttgccgcca atttgggtga ccacatattg tgaccaacca cccggagcag
    36541 caccaaggtc gacaaccgtc attcccggtt taaagagttt gtcactttgc tgtatttcat
    36601 caagtttaaa ccaggcacgg gaacgtaatc cctttttctg tgcctgttga acatatttat
    36661 cgctaaagtg ttcctgaagc cagcggctgg agctggcaga acgcttctta cctgtcattt
    36721 aactttccca tggggataac tcatcgtaac caattgcgta aatttttacg cgcctatttg
    36781 gtgatatatg ggagatggcg gtagaatgac ccgttttcaa tcccaacgta agcaaaaaat
    36841 acgatgaatc tgagtactaa acaaaaacag cacctgaaag gtctggcaca tccgctcaag
    36901 ccagttgttc tgcttggcag taatggtttg accgaagggg tgctggccga gattgaacaa
    36961 gcgttagagc accatgaact catcaaggtg aaaatcgcca ccgaagatcg cgaaacaaaa
    37021 accttgatcg tggaagctat cgtgcgcgaa accggcgcct gtaatgtaca ggtcatcggt
    37081 aaaacgctgg tgctttatcg cccaactaaa gaacgtaaaa tctcgctgcc acgctaagat
    37141 tatcctaaag ttacacacat ttgctgtgta aaacgagggg ttttccgcag gcaggagagc
    37201 aaaatgccac gctctgttcg ttgataaaag gccgcagagc ggcctttttc ctttctttac
    37261 aatacatcaa catcttgagt attgggtaat tcttacaggt attccacctt aattacttca
    37321 aattctactt cgccgcccgg cgttttgatg accacaacat catcttcttc tttgccgatc
    37381 aggccacgag caataggcga gtttacagaa atcaggtttt gtttaaagtc agcttcgtca
    37441 tcgccaacga tgcgataagt ctgttcttcg tcagaatcca gattcagcac cgttacggta
    37501 gcaccaaaaa taacgcgccc attgttgggc attttggtga catcaatcac ctgcgcgttt
    37561 gacagcttgg cttcgatgtc tttgatacgg ccttcgcaga agccctgctg ttcacgagct
    37621 gcgtggtatt cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc gatagcagca
    37681 atgatttcag gacggcgcac agatttcaga aaatccagct cttcgcgtaa tttttcagcg
    37741 ccgcgtaagg tcatcggaat agcttgcatt tgttatacct cttgaatatt cctgatgggg
    37801 caagtcttca cccaccccgg ctgttcggcc tgcccggcat aactcctcac cgggaccaga
    37861 agcaaaaaaa taccgacccg ggtacaagtc ccaggtcagc tacaattcac attttgatgg
    37921 tcattttacc ctgaagttcc cgaagggtca tcgtttactt tatagggcgt tgcgccgtag
    37981 tatgacggct cgattccagg ttgttagcgc gagattatgc gattttccag atttatcatc
    38041 ggattgacca gctgtatagc gttcagtgtt caggccgcga atgttgatga gtacgttact
    38101 caactccccg caggtgccaa ccttgccctg atggtgcaaa aagtcggtgc gtcggccccc
    38161 gctattgatt accacagtca gcagatggcg ctgcccgcca gtacccagaa agtgattact
    38221 gcgctggcgg cgttgattca actcggcccc gattttcgtt tcaccacgac gcttgaaacc
    38281 aaaggcaatg tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt tggtgccgat
    38341 ccgacgttaa aacgtcagga tattcgcaat atggtcgcga ctttgaaaaa atctggcgtc
    38401 aaccaaatcg atggcaatgt gttgatagat acctccattt tcgccagcca cgataaagcc
    38461 cccggctggc catggaatga catgacacaa tgctttagcg ctccgcctgc tgccgccata
    38521 gttgaccgca actgtttctc cgtctcgctc tacagtgccc caaagcctgg tgatatggct
    38581 tttatacgcg tggtatctta ttaccccgtt acgatgttca gccaggtacg caccctcccc
    38641 cgtggttctg ccgaagcgca atactgcgaa ctggatgtgg tgccaagcga cctgaaccgc
    38701 tttacgctga cgggatgcct gccacaacgt tctgagccgc tcccgttggc ttttgccgtg
    38761 caggatggag ccagctatgc cggtgcaatt ctgaaagatg agttaaaaca ggcgggtatc
    38821 acctggagcg gaacactgct gcgccagact caggttaacg aacctggaac ggtagttgcc
    38881 agtaaacagt cggccccgct gcacgatctg cttaagatta tgctgaaaaa gtcggacaac
    38941 atgatcgccg atacggtttt ccgcatgatc ggccatgcgc gcttcaatgt gcctggaaca
    39001 tggcgggctg ggtcggacgc cgtgcgtcag atcctgcgcc agcaagccgg tgtcgatatt
    39061 ggtaacacca ttattgccga tggttcaggg ctttcgcggc ataacctgat tgcccccgcc
    39121 accatgatgc aggtgctgca atacattgcc caacacgaca atgaacttaa ctttatctcc
    39181 atgctgccgc ttgcgggcta tgacggctct ttgcagtacc gtgcaggtct gcatcaggcg
    39241 ggcgtggatg gcaaagtctc agcgaaaacc ggttcgttgc agggggtata taacctggcg
    39301 ggattcatta ccacagcgag cgggcaacga atggcatttg tacaatatct ttctggctat
    39361 gcagtaaaac ccgcggatca gcgtaatcgc cgtattccgt tagtgcgttt tgaaagccgt
    39421 ttgtacaaag atatttatca gaacaattag tcaaaaagaa accccggcgc atggccgggg
    39481 tttcagatta ttgacaaagt gcgctttgtt tatgccggat gcggcgtgaa cgccttatcc
    39541 ggcctacaaa atcgtgcaaa ttcaatatat tgcaatcatc ttgtaggcct gataagtgta
    39601 gcgcatcagg cttatttggc attggtcatt agtgattaac gcttgtaaat gaactcaacg
    39661 ccttcttcgt cgtcttcgtc ccagtcgtca tcccagtctt catcatcctc ttcagcaatc
    39721 tcttcaagct gctgacggtg ataatcatcc cacatgaatt cgactttctc tggctgtttc
    39781 gcttcttcag cctgcacgac cgggttttca atgataaagg tcatcacatc ccagcagaga
    39841 tctttcacgc ccagtccgct cgccgcagag atcagataat atttatcttc ccagcccagc
    39901 gcttcagcga tcgctttcgc tttctcttcg gcttctgcct tatccagcag atcgatcttg
    39961 ttgaagacta accaacgcgg tttcgccgcc agatcctggc tgtatttttc cagctcgctg
    40021 ataataatac gcgcgttttc aaccggatcg gtgccgtcaa tcggatcgat atcgatgagg
    40081 tgcaacagga cgcggcaacg ttccaggtgc ttcaggaagc gaatgcccag acctgcgcct
    40141 tccgcagcgc cttcaatcag tcctggaata tcggcaacaa cgaagctctt ttcgttgtcc
    40201 attcgtacca cacccagact tggcaccaga gtggtaaacg gataatccgc cactttcggt
    40261 ttagccgccg ataccgcacg aataaaggtc gatttacccg cgtttggcat ccccaacata
    40321 ccgacgtcag ccagcagcat cagctccagc agcagctcac gcttatcgcc cggcgtaccg
    40381 ttagttttct gccgtggtgt acggttaact gacgatttga aacgggtatt gcccagaccg
    40441 tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt tagtcatatc acccatggtt
    40501 tcaccagtac cctggtcgat tacacgcgta cctaccggca ctttaatcgt cacgtcttta
    40561 ccgcgcttac cggtacaatc gcggcttgcg ccgttctgac cgcgctctgc acggaaagat
    40621 ttttcaaaac gataatcgat aagcgtgttc aggttctcgt cggcttccat ccatacgtca
    40681 ccaccatcac cgccgtcgcc gccatccggg ccgcctttcg gaatatactt ttcgcggcgg
    40741 aagctcacgc aaccattacc gccatcacct gcaacgacca gaatcgatgc ttcatcaaca
    40801 aacttcattt tattctccgt aaatcattcg cctgcgcggg gttgcgatac caccgttgta
    40861 tgcttacgta atccgcccca aatacgatga ccaatggcgg aatacatcgc gcccgcaacc
    40921 acgacaaacg caccgagata acctaaaagg tttaacatcg gtctggcgaa gaaatcgggc
    40981 caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca gtggggtgag cgtgatgatc
    41041 gcgctcacct gcgctgcctg ccagcgagcc atcgcttccg ccaaggcgcc atatcctacc
    41101 aaggtattca gtccgcaaaa aattaaacat gcgagctgcc agtggctaag ctgcgctatc
    41161 actccaggct ttgccagagg gaagagcgcg attgtacata aagtgtacag taaaaacagg
    41221 atctgcggtg aggccagccg acgcaataaa accttttgcg ccacgccata actcacccaa
    41281 accgtcgccg caccgacccc aaagataact ccccaggtgt aatcggtgag ctttgtaaat
    41341 atctcgacca gactggtgtt aaaaaacatc accagaccgc tcaggagcat caatgcccct
    41401 acaacctgag tgctgcgcat tttctctttc aggataaata cgctggcaac catcatgcca
    41461 actggcgaga gttgcccaat cacctgcgaa gcggtcggac tcaggtattg caaggatgag
    41521 ctgaacagga tgaagttccc aaacagcccg gcggtcgcca ctgccaacaa aatcaaccag
    41581 cgtggcttac gaaacacgcg taatggcggc aacctcttct tcaccgcaag aatggcacca
    41641 aggccaatac tcgccatcaa gaaacggtaa aacacgattg tcggaggttc catcacctcc
    41701 agcacctgct tcattgcgat tggcaacgcc ccccagcaaa ttgctgtggt aagcgccaaa
    41761 agaatgccaa tgcctgcctg ctgcttcata ccgtattccc tgcaaagcgc atttgcggat
    41821 taacgtcgca ccatgcgcat ttttaccggt tatcgaatgt aaaaagcccc gcaacgtgtt
    41881 gcggggcttt catccgttac cgggacgcga aaaacttatt cagcttcgat gctgataaat
    41941 ttacggtttt tcgggccttt aacttcgaat ttcactttac cgtctgcttt agcaaacaga
    42001 gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga atttggtacc acgttgacga
    42061 acgatgatgc tacccgccag aacggattcg ccaccgaaac gcttaacgcc caagcgttta
    42121 gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct ttttatgtgc catttgaaat
    42181 ctctcctcag gtcttaggcg ctgatgccag taattttcac atcagtgaac cactgacgat
    42241 ggccctgctg cttacgatag tgtttacgac gacgaaactt aacgatttta actttctcgc
    42301 cacgaccgtg agcaacaact tcagctttga ttacgccgcc atcaacgaaa ggaacgccga
    42361 ttttgacttc ttcaccgttt gcgatcatca gcacttcagc gaactcaaca gtttcgccag
    42421 ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc gcttactcgg tgttgtttac
    42481 caccactttg gaaaaccgcg tacatataaa actccgcttc cgcgcacacc ttttcaatga
    42541 ttcagagtgc gctataaata ttcacaatag ggcgcgaata ttacgcaaaa cgcacgcctt
    42601 tgacaagtgc tacagtcaat acacgaagaa aaaaacacaa cttgtacggt aacgtttatc
    42661 tgtgccattt tttcagtaca attaccctat attcctaacc ataaacccta agttgccttt
    42721 gttcacagta aggtaatcgg ggcgaaaagc ccggcttttg cgatgaattt agaaaaaatc
    42781 aatgagttaa ccgcgcaaga tatggcgggt gttaatgcgg caatccttga gcagcttaat
    42841 tccgacgtcc aactgatcaa tcagttaggc tattacatcg tcagcggcgg cggtaaacgt
    42901 attcgtccga tgattgctgt actggctgca cgagctgttg gctatgaggg aaatgcgcat
    42961 gtcaccattg ctgccctgat cgagtttatc cacacggcga ctctgctaca cgacgacgtt
    43021 gtggatgaat cagatatgcg caggggtaaa gctaccgcca acgccgcatt tggcaatgcc
    43081 gccagcgtgc tggtaggcga ttttatttat acccgcgcgt tccagatgat gaccagcctc
    43141 ggttcactca aagtgctgga agtcatgtca gaagctgtaa acgtcatcgc agaaggtgaa
    43201 gttctgcaac tgatgaacgt taacgatccg gacatcactg aagaaaacta catgcgcgtt
    43261 atctatagca aaaccgcgcg tctgtttgag gctgccgcgc agtgttccgg gattctggct
    43321 ggctgtacgc cggaggagga gaaaggcctg caggattatg ggcgctatct cggcactgct
    43381 ttccagttga tcgacgattt actcgattac aatgccgatg gcgaacagtt aggtaaaaac
    43441 gtcggcgacg atctgaacga aggtaaaccg acgctgccgc tgctgcatgc gatgcatcat
    43501 ggcacaccag aacaggcaca gatgatccgt accgccatcg aacaaggtaa cggtcgccat
    43561 cttctggaac cggttctgga agcaatgaac gcttgtggat ctcttgaatg gacgcgtcag
    43621 cgcgccgagg aagaagcaga caaagccatc gcagcgttac aggtgctccc ggacacccct
    43681 tggcgagaag cactcatcgg cctcgcgcac atcgctgttc aacgcgatcg ttaatcccct
    43741 cccttcatcc cgcgcagtgc gcgggatgat tccagtaaat tccataaaaa cactaattca
    43801 gcgctataac acctgaaaaa agggcgatga atctttatat attctgaata ttcacactct
    43861 ttacaggaac tttttagcgc aatagaccat caggagtata gtgatgcccg acagaagaag
    43921 tgttctgaat gaaagcgact aacttaagga gtgaggaaag atggaaagta atttcattga
    43981 ctggcatccc gctgacatca ttgcaggttt gcgcaagaaa ggaacgtcaa tggcggcgga
    44041 atctcgcaga aatggtttga gttcctcaac gctggcaaac gcattatcgc gcccatggcc
    44101 gaaaggagag atgattattg cgaaagccct gggaactgac ccctgggtta tctggccatc
    44161 acgctaccat gatccgcaga cccatgagtt tatcgacaga acgcagttga tgcgtagcta
    44221 cactaaaccg aaaaaatgag tggtctggcg gtagccccgc gaacggggct gccagctctc
    44281 agacgattac tcgcctttca cacgctcaat atttgcacct aaagcgcgca gtttgtcttc
    44341 aatgcgttcg tagccacgat cgatgtgata aatacgatca accaccgtcg tcccttccgc
    44401 aatacagcca gccagcacca ggcttgctga tgcacgcaga tcggttgcca taacctgtgc
    44461 accagaaagt ttttcaacac catgacaaat aacggtattg ctttcgattt cggcgtgcgc
    44521 gcccatacgg ctcagctctg gcacatgcat aaagcggttt tcaaagaccg tttcggtgat
    44581 gaacccggtc ccttcagcca ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg
    44641 gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc catgcatatc
    44701 caggctaatc cagtcttcgc cgacttcgat gtccgctcca gcatcacgca gtttcgccag
    44761 cacggcgtcc agagtatctg gctgcgcgtt acggcagata attttgccac gagagatcgc
    44821 cgccgccacc aggaaagtac cggtttcgat acgatccggc agcacgcgat agacaccgcc
    44881 gcctaaacgt tccacacctt cgatgacgat acgatcggtg ccctgaccgc taattttcgc
    44941 acccagcgta atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg ctgcgttttc
    45001 aataatcgtg gtgccttccg ccagggttgc agcacacatg atggtcaccg ttgcgccaac
    45061 gctgacttta tccatcacga tatgtgcgcc tttcagacga ccatcgacgg aagctttaac
    45121 gtaaccttct tccagtttga tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc
    45181 aaccggacgc gcaccgatcg tacaaccgcc aggtagtgaa acttgcccct gaccaaagcg
    45241 cgctaccagc ggccccagcg cccagataga agcacgcatg gttttaacca gatcgtaagg
    45301 tgcgcagaat acattaacgt cgcgggcatc aatatgcaca gaaccattac gttctacttt
    45361 cgcacccagc tggcttagca gtttcattga tgtatcgacg tctttcagtt tcgggacgtt
    45421 ctggatctct accggttctt ccgccagtag tgcggcaaaa agaataggca gagcagcatt
    45481 tttagcgccg gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt
    45541 atccatttag tttgttctca gttaacaatt catatccgct accggcgaat cgcccatagc
    45601 tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg ctttgatcga
    45661 cacagcatga atgcggttat ccgcaatata ttccatcagc gggccataga ccgtctgctg
    45721 ttttttaacc cgactcatac cgtcaaacaa ctcacccacg gcaataacct gaaagtggct
    45781 gccatcgccg gaaacgtgga cttcctggag ggagagagcg ttcatcaaca cgctctgaat
    45841 ttcattattt tccatgggat cttcaatcat cagttaataa accagcgaaa catcttagag
    45901 caaagttgcg ctggcataaa taagcaaaaa gcctcgctga taaatcagac aaggctcgac
    45961 ttgcaggcag gtttgccgga caggcggtta acgccatatc cggcctgaaa aaatttaacg
    46021 aggcagaaca tcagcaggca aattatacaa tttcgcgagg gtatacactt tgtcgtttac
    46081 cccctgaagc gtcacgttgt tgccctgctt tttcgccaga tcgataagat ggagcagcag
    46141 tgccagtccc cccgtatcca cgcgggacac acggctaaga tcgatgcagg taatcccctt
    46201 caccgcctcc tcacgcattt cccaaagcgg tagcagaacg tcctgatcca gctctccgga
    46261 taacgccagc gtgtcacccg tctgcatcca gctcagtgac tcgctcatta ttttttctct
    46321 tccagagtga ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct
    46381 ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg tgatcatact gacgccttca
    46441 gcaatcatgt cgtaagcttg ccagtttccc gtctgggagt ttttacgcca ctggaagtcc
    46501 agacgcaccg gcggacggcc attcgggtca ataatggtaa cgcgaatagg cacaatggtt
    46561 ttatcgccca gcggctgttc tggcgcaatc tgataggttt gaccgtgata catcgccagc
    46621 gcctgaccgt aagcctgctt caggtactca cggaaagcgg caaagtaggc ttcacgttga
    46681 gcaggggtcg cactcttgta atactggccc agcaccagcg caccggcgta tttcacctgt
    46741 acgtatggca gcagttcctg atcaacaatg gtacgcagat aatccgggtt ggcccgaatt
    46801 tgcggttgct cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc catcagctta
    46861 taaggattgg tctggtctgc cgcggttgcc gcactcagag gtgcaatcac cagcaaagcg
    46921 accatcatta aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt tgtacccaca
    46981 ggttcagtgg tttcattatt acctggcgca gcagctggcg catcgccact attcttattg
    47041 tcatcgcctt tactaccgta aaggaactga ccaatgagat cttccagcac catcgcagac
    47101 ttggtgtcct gaattgtatc gccatccttc aggatagcag tccccagttc cgggtcttca
    47161 aaaccgacgt ttaatgccag atattgttct cccagcaggc cggaagtacg aatgctcagc
    47221 gaactggtat caggaatgtg gttataacgt tgttcaattt ccagcgttac gcgcggcaga
    47281 taggttttcg ggtccagcgt aatatccgcc acccggccca caacaacacc accaatgctg
    47341 accggagagc gcgctttcag gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc
    47401 gattcagtac gtatggacgt cacgttcgcc gccttcaggc aaacaaacag cgccgccagc
    47461 agtgctgcta ataaaaagat acccacccaa atttcatttt ttttcgtttg catgaactca
    47521 attcccaaac atcaatgcgg tcagcacaaa atccagcccc agaacagcca gagacgagtg
    47581 gacaacggtg cgagtggttg cccggctaat cccggcagac gtcgggatgg cgtcgtagcc
    47641 gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt
    47701 gaccagatcc atacgccagt cgacggcatt ttgcattgcc gaccagaaga acccgctatc
    47761 aatgcctttc caactgacgc cgaccagcga tccgccccag atccccacgg cgacgaagat
    47821 aaccgtcaac agtggtaatg aaataacccc agcccagaaa cggggagaaa taacccgacg
    47881 cagcggatcc accgccatca tctccatact ggagagttgc tctgtagcgc gcatcaggcc
    47941 gatttctgcg gttaacgccg aaccagcacg cccggcaaac aacagcgcgg caaccaccgg
    48001 ccccagttca cgcagtagcg ataacgccac cagcataccc agactggttt ccgcgctata
    48061 agtggtcaga accagataac cttgcagccc caacaccatt ccgatgaaca cgccagaaac
    48121 tacaataatc agcatcgaca ggacgccgac attatagagc tggcgcacca gcagcggcgc
    48181 atgtttgcga aattccggtt tgccgaccag cgcattgaat aacattaacc cggcccgccc
    48241 gaacgttctc agggttttaa tccctttatg tccgagcgac gccagcgcat ttaacagcat
    48301 gagtggctta actccctggt aaaagatcag cgtgataatc gccggcagga tagcggaacg
    48361 gaacaggccc gtcagctatc ccgtccagaa actgacgtac gcgcggatca ggattcgtct
    48421 gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc cgccaggatc caggcgtgat
    48481 ccgcaatact taacacttcc ggcacatcgt gagaaaccac cacacaagtc acgcccagcg
    48541 cgctgttcag ctcagaaatc agcttcacca gtacgcccat ggtgatggga tcttgcccaa
    48601 caaaaggttc atcaaacatg atgagatccg gctccagcgc aatcgcacgc gccagcgctg
    48661 cacgccgcgc catcccaccg gaaagttcag aaggcattag tttagccgct ccacgcagcc
    48721 ccacggcctc cagcttcatc atcaccgtac tatgcaacaa tggcgcggga agttgggtat
    48781 gttcgcgcag tggataggcg acgttgtcaa atacgttcat atcagtgaac aacgccccgg
    48841 actgaaataa catgctcatc cgtttacgca ctgtatacag gcgcgaacga gacatcgccg
    48901 gaatattctc accatcgaaa aggatctcac catgatctgg tgcgatttgc ccgccaatca
    48961 gacggagtag cgtcgtttta ccgatgcccg atggccccat gatcgccgtg atcttccctc
    49021 gcggcacggt cagggaaata ttatcgaaga tgcagcgatt gccacgcgta aaactgacat
    49081 cgcgcatatc gactaaattc gccacagact gctccataat tcacccttcg tcttgcgttg
    49141 attttctaag catggcgctc aatttaacct tgaacccaac atatttacag aatattaccc
    49201 gccgtggtta gcgaaagctg gcatttgttt tactttttag ccgcataaag tcaaaattaa
    49261 gaatccgtta cggctttctg aaaatcttca gcggaccggc gagtatacct gaagaaagga
    49321 cgttagatgc ttttagctac ggcactgtta attgttggtt tacttttggt cgtttacagt
    49381 gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa cctttggcat cccgccgctg
    49441 atcatcggca tgacggtggt cagtattggt acatcgttac cagaaatcat cgtctcgctt
    49501 gccgcgtctc tgcacgaaca acgcgattta gccgttggta cagccctcgg ctcaaacatt
    49561 atcaatatat tgctgatcct cggtctggct gcgctggttc gtccttttac cgtccattcc
    49621 gatgttctgc gccgtgaatt acccttaatg ttgttggtca gcgtggtggc cggttccgta
    49681 ctctatgacg gacaacttag tcgaagcgat ggtatctttc tcctctttct ggctgtgcta
    49741 tggctgctgt tcattgttaa acttgcacgt caggggactg acagcctgac cagagagcag
    49801 cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct cggcattgcg
    49861 cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt gctggcgaat
    49921 tactttgcca tcagcgagtt gacgatggct ctgacggcaa ttgctatcgg aaccagtctg
    49981 ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat tgctgtcgga
    50041 aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata
    50101 acgccaggag agattgatcc actggcgtac agtcgtgact acagcgtgat gttgctggtg
    50161 agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg
    50221 gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt atcgccaata
    50281 ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg gttttgactt
    50341 tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca
    50401 atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt
    50461 tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg caacgtttgc
    50521 cagcaccggt acaccttcat ttttcgtcca tcctagtgaa gccgcgcatg gtgatttagg
    50581 catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat ccagcgaaat
    50641 cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg
    50701 cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga
    50761 agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg ttatgggcga
    50821 tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt ttgcgctctc
    50881 acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac
    50941 gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt
    51001 tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga ttgaaggcat
    51061 ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc gtcagttaag
    51121 tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc tggccgttga
    51181 ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg atggcgacca
    51241 tttactcggt gtgttacata tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa
    51301 ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc
    51361 gccgacgtta tggcaaaagc agagaacatt cgtctgctga tcctcgacgt tgatggcgta
    51421 ctgtcagatg gcctgattta tatgggcaat aacggcgaag agctgaaagc gttcaatgtt
    51481 cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc tatcattacc
    51541 gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac tcacttgtat
    51601 caggggcagt caaacaaact gatcgccttt agcgatttgc tggaaaaatt ggcgattgcc
    51661 ccggaaaatg tggcttatgt tggcgatgat ctcatcgact ggccggtaat ggaaaaagtg
    51721 ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc cgattacgtg
    51781 acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt actcctggcg
    51841 cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga cgttgggtta
    51901 tcattgtgct atcactggcg gttctggtga tgatcggcat taatatggcc gaaaaagacg
    51961 ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag catacggaca
    52021 cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa cacgttgaat
    52081 attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg tttgataagg
    52141 ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat gaccggatgc
    52201 tctatttata tggacacgtt gaagtcaacg cacttgtgcc agactctcaa cttcgcagaa
    52261 tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct gaagacctcg
    52321 tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc aacttacgca
    52381 gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa
    52441 ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc ggcatttgcc
    52501 gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc tcttgatatg
    52561 caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac catcaaaatt
    52621 aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga agtaattgac
    52681 ggatacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc cgttgaaggt
    52741 cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac gggtaatgct
    52801 tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct ggtgaaagag
    52861 cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct ggtgccgtcg
    52921 cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg taattaattc
    52981 gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta
    53041 gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg gccaaacggt
    53101 gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac
    53161 atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt
    53221 atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta cgataacctg
    53281 atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga ccgcgcgaac
    53341 gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca gtcactctcc
    53401 gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt
    53461 ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat taaacgcatc
    53521 attgaacacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa
    53581 acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat cgcccacggc
    53641 acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg ggaagacttc
    53701 agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt ctgaacatga
    53761 agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag ctccaacagg
    53821 caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag caggcgctgg
    53881 agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc
    53941 aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg ccggaagagc
    54001 tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc ggcaccagcg
    54061 gtgactacat tgacgacgag ctgccggtct accagggcga aacgacgcag accttgcagg
    54121 attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc gctattgcta
    54181 cctctatcgt cgatgccgtt gatgaaaccg gttatctgac tgtcccgctg gaagatattc
    54241 tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc cttaagcgga
    54301 tccaacggtt tgacccggtc ggtgtagcgg caaaagatct gcgtgactgc ctgctaatcc
    54361 aactctccca attcgataag accacgccgt ggctggaaga ggccagattg atcattagcg
    54421 atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc acgcgtctga
    54481 aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg cgccccgggc
    54541 agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg cgtaagcata
    54601 acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc
    54661 actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt atccgcagca
    54721 atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat acgctactgc
    54781 gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt gaagaatata
    54841 tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa tcgacgatat
    54901 ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa ctgaagtatt
    54961 tcttttccag tcacgtcaat accgagggcg gcggcgaagc ttcctccacg gcgattcgtg
    55021 cactggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc gacagcaagt
    55081 taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt gcgaagtacc
    55141 gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat
    55201 aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac cgaggcactg
    55261 cgcgaatttg ttacagctaa atttgccaaa cttgagcaat attttgaccg aatcaaccaa
    55321 gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac actgcatgta
    55381 aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc
    55441 ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa acaacactaa
    55501 ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta
    55561 cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg tgaaattatg
    55621 acaaataatg atacaactct acagcttagc agtgttctta acagggaatg tacgcgaagc
    55681 cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa
    55741 caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga aaaaatgggc
    55801 agtaccggta tcggcaatgg tattgccatt ccgcatggca aacttgaaga agatactctg
    55861 cgtgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc catcgacaac
    55921 caaccggtgg acttactttt tgccctgctg gtgccggcag accaaactaa aacgcacctg
    55981 catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc
    56041 gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg tactccggat
    56101 gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca gtacatggta
    56161 ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa
    56221 gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga tctggctcga
    56281 actctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa tatgccggag
    56341 tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc accgcaacta
    56401 ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac gcgccgactg
    56461 catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg
    56521 ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat gtccgttcac
    56581 gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga actgaccatg
    56641 gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta cgtctttgac
    56701 gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac aggtcttgat
    56761 aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat ctaccagacg
    56821 cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag ctacctgacg
    56881 gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga gcaactggca
    56941 gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt
    57001 aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg catgcccggc
    57061 ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg
    57121 acgaaggcac cgaggctgaa gccaacagcg ttattgcgct gctgatgttg gattctgcca
    57181 aaggacggca gattgaagtt gaagcgaccg gtccacagga agaggaagca ctggccgccg
    57241 ttatcgccct ctttaattct ggttttgatg aagattaatc ttcatcactt tgacatacaa
    57301 ctcccttcaa gctcccccga caataagaaa atcacgtact gaaatcgttc tcaatcaacg
    57361 tcatttgtac attttgtgcg cttttcactt tcagaagaac cttaagaaaa ccttaagagg
    57421 cattgtttag gttttgttta agttaatcga ccatactgga gatcgtcaga aaatatttcc
    57481 aggagatggc atgattcgct tatctgaaca aagtcccctg ggcaccggaa gacatcggaa
    57541 atgttatgcg catccggaag atgcccaacg ctgtatcaag attgtctacc atcgtggcga
    57601 tggtggcgat aaagagatcc gccgtgagtt aaagtactac gcgcatcttg gtcgccggtt
    57661 aaaagactgg agtggaatac cgcgctatca cggtaccgtt gagacggatt gcggaaccgg
    57721 ttatgtctac gatgtgattg ccgattttga cggcaagcct tccattacgc tgaccgaatt
    57781 tgctgaacaa tgccgttacg aagaagacat tgcacaactg cgccagctac tgaaacagct
    57841 gaagcgttat ttgcaggata accgtatcgt gacgatgtcg ctgaagccgc agaatatcct
    57901 ctgtcaccgc atcagtgagt cagaggttat cccggtcgtc tgcgacaaca ttggtgaaag
    57961 cacattgatt ccactggcga cctggtcgaa gtggtgttgt ctgcgtaagc aagagcggtt
    58021 atggaaacga tttatcgccc aaccggcgct ggctattgcc ctgcagaaag atctgcaacc
    58081 tcgtgagagc aaaacgctgg ccctcacttc gcgcgaagct taatccagct ggtgttgttg
    58141 cataaacggc tcaccgccta actgatacat ctgccgtaaa atccacgcct gacggctacg
    58201 cacgtagccc gatggcgagg agactttgaa acgaagtgga ttaggtaata cagctgccag
    58261 taatgcagct tccgaccggg taagtttgct cgcgggtttg tggaaataac gttgtgccgc
    58321 agcttcgacg ccaaacacac cgtcgccaaa ttcggcgata ttcaggtaaa ccgtcaggat
    58381 acgctttttg ctccagactg tttctatccc cagcgttaat ccagcttcca gcccttttcg
    58441 tacccagcta cgcccatccc ataaaaagag attttggccg tctgctgaga aatcgttgaa
    58501 gcaccgcgaa tacagttttc attgcgctcg ttgtgcgcca gggctttctc aatggaagcg
    58561 acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac cgccagtccc
    58621 atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta acgaaaattg
    58681 ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg aacaggcgca
    58741 acgctaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat taaccgcaaa
    58801 agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca gccagaacca
    58861 gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca
    58921 tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca tcgacaggac
    58981 acggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata tcagtaccga
    59041 tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca atacacataa
    59101 aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa
    59161 cggtgcattc gctaccaaga ctggcaaaat tgcttaaatt cttcgccgcg ccaaaccccc
    59221 ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga cggatttcac
    59281 cgcgcgttat tcgtgccgct tcaatcagca cattgcgcgt ttccgtcatc gcttcgccag
    59341 ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc gcctgcgcac
    59401 cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga
    59461 cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt tcgcaattga
    59521 ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa ctgattcatg
    59581 taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc
    59641 ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg ttggcgcagt
    59701 attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac ccacgctttc
    59761 agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc accgacgtta
    59821 tcttcccagg cgggaaggtc aggagactga atttgctgac ccagctgttg cagatggcgt
    59881 aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat gcctttttta
    59941 tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat cttctcaaac
    60001 accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg ttccagcatg
    60061 ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt actctcctca
    60121 tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac cgaaagcggc
    60181 ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt cagcacgtta
    60241 gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga
    60301 gagatatcca gcccggtcat atctggcaac tgaatatcca gcaacaccag gtcgtattcg
    60361 cccggtttaa acatctccag cgccgccttg ccggtcatgg cgacatcaac gctgttacct
    60421 aatttttcca gcacagaacg cgcgacaatc acgttcagtt caatgtcttc caccagcagc
    60481 acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac ctcttctgcc
    60541 accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc actggtaacc
    60601 gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc aataccggtg
    60661 ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc gaagatttta
    60721 tccagctcat cctgcggaat gccgatacca gaatcttcca cttcaaaatg cagcatatcg
    60781 ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt gacggcgtta
    60841 ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt
    60901 aatggcagcg taggctccag gttaaagcgc aatccttttt gttgcgcctg caatgcggag
    60961 agattttcca gatcggcaag gaagctggtg aaatcaaccg gctgattatc aagctggacc
    61021 ttgcgccgtt ccatcttatc catgtcgata atatcgttaa agatattccc cagcgtgacg
    61081 gccgaaacat ggatagtttt gagatatttt tcctgctcgg cggtgagttc ggtatccagc
    61141 agaatgcggc tcaggccgac gataccgttc agcggtgtac gcaattcgtg actgatggtg
    61201 gaaataaacg tcgttttgtc gcggctggcg cgttcaagcg catcctgata ccgcttacgc
    61261 tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac
    61321 ggcactttac ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag ccactgttca
    61381 taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac ttttgcggcg
    61441 gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc gctttttccg
    61501 gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt acgataaaaa
    61561 accaagtcgg gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg ctcgagctga
    61621 atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt ttcctgcagt
    61681 tctgcttccg ctttttcacg aacggcaatt tcctgattta gctgggcaat attatctttt
    61741 aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg caccagccgt
    61801 gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc
    61861 cacggcgtaa tcagcaaacc aaagaagata gaacgaataa catcaatgct ttcgacctga
    61921 ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac gagggccagc
    61981 gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac ataatactgc
    62041 gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc ccaataatac
    62101 ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc tcaacgacct
    62161 actccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca tgttcattca
    62221 gatagcgatc cagctcgacc attcccgtcc atcgattttc gcaccacagc ggagcaagca
    62281 gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt ggcgtatggc
    62341 gaatcatttc tccggcagtg agcgtataat cctccagttc aataccgttc aaacgccccg
    62401 cttcccaggc tttcgccata atgctgcctt tcacaatatg cagcggatgc agctttatgc
    62461 cgtccacgct ggtttcaacc actcgctcca gcgtttgcaa acattcagcc tgcccttcgc
    62521 caggcaggcc gacaattaag tgggaacaca ccttcagccc gcgctgacgt gccagctggg
    62581 ttgtacgctg ataacaggca aaatcatggc cgcggttgat gcgatgcagt gttttgtcgt
    62641 gggcggtttg tagccccagc tccagccaca cttcgtaacc ctggtcctta tattcgcaaa
    62701 gcagatccag caccacatcc ggcacgcagt ccgggcgggt accaacacac aaaccgacaa
    62761 tattggcctg gctcaccgcc tgctgataca tagaacgcag cacctgaact tccgcaaagg
    62821 tgctggtata cgcctgaaag taggccagat agcgtttagc gcggttaact aaattcgcct
    62881 ggtgcgccag ttgctcggca atggaacgat gctgctgcgc ttcatcggca aacgaggcaa
    62941 cattacagaa tgtgcagcca ccacgcccga tggtaccgtc acggttaggg cagctaaaac
    63001 cgccgtgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga tcaccaccaa
    63061 acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga aaagaggcca
    63121 aagcctgcca tttttagctc aattcggcga tgacctgggt caatcgtccc ggcctgcttt
    63181 tatcaactgc ataatcaatc aaaattaccg aaatttcatg cacaatcaca taaatcactt
    63241 ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa aacagtagca
    63301 tgaaacgtca ttaccaatta aggcagtata aaatgctggt tttctcgtca gttcaaggca
    63361 ggataagggt taacacgcct ttatgacagt caggaattga ctgtttctct aacgactctc
    63421 cttttagcct taaagataaa atctatttta atttcagcta gttaataaag aattttgcgc
    63481 taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt tcccgataag
    63541 ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt atgcagtaat
    63601 tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg gcgaatgcga
    63661 ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt
    63721 tcggaagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta cgataaatcc
    63781 cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga acctagccac
    63841 aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg cgcgattctc
    63901 gccgatggta aaaccggcga cggttgcggc ttgctgttac aaaaaccgga tcgctttttt
    63961 cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa actacgctgt cgggatgctc
    64021 ttcctgaata aagatcctga actcgccgct gccgcacgcc gcatcgttga agaagaactg
    64081 caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg
    64141 ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa tttttgtgaa cgccccggca
    64201 ggctggcgtc cacgcgatat ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag
    64261 cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt cgaatctggt gaacatctat
    64321 aaaggtctgt gtatgccggc ggatctgccg cgcttttatc tggatcttgc ggacctgcgt
    64381 ctggaatcgg ccatttgcct gttccaccag cgcttctcca ctaacaccgt accgcgctgg
    64441 ccgctggcgc aaccgttccg ctatctggcg cataacggtg aaatcaacac catcaccggt
    64501 aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgctgat ccctgacctg
    64561 cacgacgccg cgccattcgt caacgaaacc ggttctgact ccagttccat ggataacatg
    64621 ctggaactgc tgctggcagg cgggatggat atcatccgcg ccatgcgcct gttagtgcca
    64681 cccgcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt tgactttaac
    64741 tccatgcata tggagccgtg ggatggtccg gcgggcatcg tgatgtccga tggtcgcttc
    64801 gctgcctgta acctcgaccg taacggtctg cgtccggcac gctacgtcat taccaaagac
    64861 aagctcatca cctgcgcctc tgaagtcggt atctgggatt accagcctga cgaagtggtc
    64921 gaaaaaggcc gcgtcgggcc aggcgaactg atggtgatcg acactcgcag tgggcgtatt
    64981 ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc acccttataa agagtggatg
    65041 gagaaaaacg tccgccgact ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc
    65101 cgcgaactgg acgacgacac gcttgccagc taccagaaac agtttaacta cagcgcggaa
    65161 gagctggact ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt cggttcgatg
    65221 ggcgatgata ccccattcgc cgtgctctcc agtcagccgc gcattattta cgactacttc
    65281 cgccagcagt ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt
    65341 atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc agagggccag
    65401 gcgcaccgtt taagctttaa atcgccgatt ctgctctact ccgatttcaa acagctcacg
    65461 acgatgaaag aggagcacta ccgcgcagat acgctggata tcacctttga cgtcactaaa
    65521 accacgctcg aagcgacagt caaagagcta tgcgacaaag ccgaaaaaat ggtacgtagc
    65581 ggcaccgtgt tgctggtgct ctccgaccgg aatatcgcta aagatcgcct gccggttccg
    65641 gctccgatgg cggttggcgc gatccagacc cgtctggtcg atcaaagcct gcgttgcgat
    65701 gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc cgtgttgctg
    65761 ggtttcggcg cgacggctat ttatccgtac cttgcctatg aaacgctggg ccgcctggta
    65821 gacacccacg cgattgccaa agattatcgt accgtgatgc tcaactaccg taacggcatc
    65881 aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc ctcttaccgc
    65941 tgctcgaaac tgtttgaagc ggtcggtcta cacgatgatg tagtgggcct gtgcttccag
    66001 ggcgcggtca gccgcattgg tggggcaagc tttgaagact tccagcagga tctgctgaat
    66061 ctgtcgaaac gtgcctggct ggcgcgcaag ccgattagcc agggcggcct gctgaaatac
    66121 gtccacggcg gcgaatacca cgcctacaac ccggacgtgg tgcgcacgct gcaacaggcg
    66181 gtacaaagcg gcgagtacag cgactatcag gaatacgcga agctggttaa tgagcgtccg
    66241 gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt caacattgct
    66301 gatgttgaac cggcaagcga actgtttaaa cgctttgata ccgccgcgat gtctatcggc
    66361 gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaatagcat cggcggtaac
    66421 tcgaactccg gtgaaggcgg cgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc
    66481 atcaagcagg tggcttctgg tcgcttcggc gttactccgg cgtatctggt caatgccgac
    66541 gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg aaggtggtca gttgccgggc
    66601 gacaaagtca ctccttacat cgccaaactg cgctattcgg tgcccggagt gacgctgatc
    66661 tccccgccgc cgcaccacga tatctactct atcgaagact tagcgcagct tattttcgac
    66721 ctcaagcagg ttaacccgaa agcgatgatc tccgttaagc tggtgtccga accaggcgtt
    66781 ggcaccatcg cgactggcgt ggcgaaagct tatgcggact tgatcaccat cgcgggctat
    66841 gacggcggca ccggcgcaag cccgctgtcg tcagtgaaat atgcaggctg cccgtgggaa
    66901 ctcggtctgg tggaaaccca acaggcgctg gttgccaacg gcttgcgtca taaaattcgt
    66961 ttgcaggttg atggcggcct gaaaacgggt gtcgatatca tcaaagcggc gattctcggc
    67021 gcagaaagct tcggcttcgg cactggccca atggtggcgc tcggctgtaa atatctgcgt
    67081 atttgccacc tgaacaactg cgccacgggt gtagcaactc aggatgacaa actgcgtaag
    67141 aatcactatc acggcctgct gttcaaggtg actaattact ttgagtttat cgcccgtgaa
    67201 acccgcgagc tgatggcaca gctgggagta acgcgtctgg tggatctgat tggtcgcacc
    67261 gacctgctga aagagctgga cggtttcacc gccaaacagc agaaactggc gctgtcgaag
    67321 ctgctggaga ctgccgagcc acatgcaggt aagtcactct actgcactga aaacaacccg
    67381 ccgtttgata acggcctact gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat
    67441 gaacgccaga gcaaaacctt ctggttcgat atccgcaaca ccgaccgttc tgtcggcgcg
    67501 tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggcagc cgatcctatc
    67561 aaagcgtact tcaacggcac cgcaggccag agtttcggtg tgtggaacgc gggcggcgtg
    67621 gaactgtacc tgaccgggga tgccaacgac tatgtcggta aaggcatggc gggcggctta
    67681 atcgccattc gtcctccggt tggttccgcc ttccgcagcc atgaagcaag cattatcggc
    67741 aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa
    67801 cgtttcggcg tgcggaactc cggtgctatc accgtggtag aaggcattgg cgacaacggc
    67861 tgtgaatata tgacgggcgg tattgtctgt attctgggta aaaccggcgt taacttcggc
    67921 gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt ccgcaaacgc
    67981 gttaacccgg aactggtcga agtcttaagc gtcgacgatc tggcgatcca tgaagagcat
    68041 ctgcgcggtc ttatcaccga gcatgttcag cataccggtt ctcagcgcgg tgaagagatt
    68101 ctggcgaact ggtcaacctt cgccacgaaa ttcgcgctgg ttaaaccgaa gtccagtgat
    68161 gtaaaagcac tgctgggtca ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag
    68221 taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca gcgcgttgat
    68281 ccgccaaaga aaccgctgaa gatccgtaaa attgagtttg ttgaaattta cgagccgttt
    68341 tccgaaggcc aggccaaagc gcaggctgac cgctgtctgt cgtgcggcaa cccatactgc
    68401 gagtggaaat gcccggtaca caactacatc ccaaactggc tgaagctcgc caacgagggg
    68461 cgtatttttg aagcggcaga actgtcgcat cagaccaaca cactgccgga agtatgcgga
    68521 cgtgtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ccctgaacga tgagttcggt
    68581 gcggtgacca tcggcaacat tgagcgttat atcaatgata aagcgttcga aatgggctgg
    68641 cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat cggtgcaggc
    68701 ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc cgttgtcttc
    68761 gaccgtcatc cagaaattgg cgggctgctg accttcggta ttccggcctt caagctggaa
    68821 aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga attcaaactc
    68881 aataccgaag tgggccgcga cgtacagctg gacgatctgc tgagtgatta cgatgccgtg
    68941 ttccttggcg ttgggactta tcagtcaatg cgcggcgggc tggaaaacga agacgccgat
    69001 ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat gggctttggt
    69061 gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtagt acttggcggt
    69121 ggcgacactg cgatggactg cgtgcgcacg tctgtacgcc agggggcaaa gcacgttacc
    69181 tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg
    69241 cgggaagaag gcgtagagtt caaattcaac gtccagccac tggggattga agtgaacggt
    69301 aacggcaaag tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc ggatgccaaa
    69361 ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata tcgttccggc agatgcggtg
    69421 atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca cagcgtcgag
    69481 ctggattcac aaggccgcat catcgctccg gaaggcaacg acaacgcttt ccagaccagc
    69541 aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt cgttaccgcc
    69601 atcgccgaag gtcgcaaagc ggcagacggc attatgaact ggctggaagt ttaagctctg
    69661 tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat tctcctttct ttctggaaat
    69721 cgcctcgcat aatctgaaga aaaccaatgt aatacgctca tctggattgt taccggtaga
    69781 tgcccggcgt atagctgatt ccattattca acgcagcgcc atataaaggg acagataatg
    69841 gaatcgctct cagagagaac ctcaacaggc taccagcaaa tccacaacgg cattattcat
    69901 ctggtcgata gcgcccgaac ggaaacggta cgtagcgtta acgcgctgat gaccgcgacg
    69961 tactgggaaa ttggccgacg gattgtcgaa tttgaacaag gtggtgaggc cagagcagct
    70021 tatggtgcac agttgattga tcggctatcg aaggatttaa gtcgacggta taaacgtggg
    70081 gtctc
//
LOCUS       DAJVPP010000005        66900 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVPP010000005 DAJVPP010000000
VERSION     DAJVPP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.005"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(66..1070)
                     /gene="cra"
                     /locus_tag="OQM34_000342"
     CDS             complement(66..1070)
                     /gene="cra"
                     /locus_tag="OQM34_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS1450919.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1153..1239)
                     /locus_tag="OQM34_000343"
     CDS             complement(1153..1239)
                     /locus_tag="OQM34_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1450920.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1251..1742)
                     /gene="ilvN"
                     /locus_tag="OQM34_000344"
     CDS             complement(1251..1742)
                     /gene="ilvN"
                     /locus_tag="OQM34_000344"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1450921.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1745..3469)
                     /gene="ilvI"
                     /locus_tag="OQM34_000345"
     CDS             complement(1745..3469)
                     /gene="ilvI"
                     /locus_tag="OQM34_000345"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1450922.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3588..3632
                     /locus_tag="OQM34_000346"
     CDS             3588..3632
                     /locus_tag="OQM34_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1450923.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3787..4731)
                     /gene="leuO"
                     /locus_tag="OQM34_000347"
     CDS             complement(3787..4731)
                     /gene="leuO"
                     /locus_tag="OQM34_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1450924.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5391..5477
                     /gene="leuL"
                     /locus_tag="OQM34_000348"
     CDS             5391..5477
                     /gene="leuL"
                     /locus_tag="OQM34_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1450925.1"
                     /translation="MTHIIRFIGLLLLNASSLRGRRVSGIQH"
     gene            5570..7141
                     /gene="leuA"
                     /locus_tag="OQM34_000349"
     CDS             5570..7141
                     /gene="leuA"
                     /locus_tag="OQM34_000349"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1450926.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7141..8232
                     /gene="leuB"
                     /locus_tag="OQM34_000350"
     CDS             7141..8232
                     /gene="leuB"
                     /locus_tag="OQM34_000350"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1450927.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8235..9635
                     /gene="leuC"
                     /locus_tag="OQM34_000351"
     CDS             8235..9635
                     /gene="leuC"
                     /locus_tag="OQM34_000351"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1450928.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9646..10251
                     /gene="leuD"
                     /locus_tag="OQM34_000352"
     CDS             9646..10251
                     /gene="leuD"
                     /locus_tag="OQM34_000352"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1450929.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10300..11274)
                     /locus_tag="OQM34_000353"
     CDS             complement(10300..11274)
                     /locus_tag="OQM34_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1450930.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALARVELPSENALSMQGDWQDSNVRMLFVASTLMW
                     TCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVI
                     AVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLF
                     TNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV"
     gene            complement(11376..11507)
                     /gene="sgrT"
                     /locus_tag="OQM34_000354"
     CDS             complement(11376..11507)
                     /gene="sgrT"
                     /locus_tag="OQM34_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1450931.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11596..13251
                     /gene="sgrR"
                     /locus_tag="OQM34_000355"
     CDS             11596..13251
                     /gene="sgrR"
                     /locus_tag="OQM34_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1450932.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13285..13384
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13415..14398
                     /gene="thiB"
                     /locus_tag="OQM34_000356"
     CDS             13415..14398
                     /gene="thiB"
                     /locus_tag="OQM34_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1450933.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14374..15984
                     /gene="thiP"
                     /locus_tag="OQM34_000357"
     CDS             14374..15984
                     /gene="thiP"
                     /locus_tag="OQM34_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1450934.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGIRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            15968..16666
                     /gene="thiQ"
                     /locus_tag="OQM34_000358"
     CDS             15968..16666
                     /gene="thiQ"
                     /locus_tag="OQM34_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1450935.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16780..17544)
                     /locus_tag="OQM34_000359"
     CDS             complement(16780..17544)
                     /locus_tag="OQM34_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1450936.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17630..18508)
                     /gene="araC"
                     /locus_tag="OQM34_000360"
     CDS             complement(17630..18508)
                     /gene="araC"
                     /locus_tag="OQM34_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1450937.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            18847..20547
                     /gene="araB"
                     /locus_tag="OQM34_000361"
     CDS             18847..20547
                     /gene="araB"
                     /locus_tag="OQM34_000361"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS1450938.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSMVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20558..22060
                     /gene="araA"
                     /locus_tag="OQM34_000362"
     CDS             20558..22060
                     /gene="araA"
                     /locus_tag="OQM34_000362"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1450939.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22260..22955
                     /gene="araD"
                     /locus_tag="OQM34_000363"
     CDS             22260..22955
                     /gene="araD"
                     /locus_tag="OQM34_000363"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1450940.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23030..25381
                     /gene="polB"
                     /locus_tag="OQM34_000364"
     CDS             23030..25381
                     /gene="polB"
                     /locus_tag="OQM34_000364"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1450941.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25546..28452
                     /gene="rapA"
                     /locus_tag="OQM34_000365"
     CDS             25546..28452
                     /gene="rapA"
                     /locus_tag="OQM34_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1450942.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28464..29123
                     /gene="rluA"
                     /locus_tag="OQM34_000366"
     CDS             28464..29123
                     /gene="rluA"
                     /locus_tag="OQM34_000366"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1450943.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29240..30055)
                     /gene="djlA"
                     /locus_tag="OQM34_000367"
     CDS             complement(29240..30055)
                     /gene="djlA"
                     /locus_tag="OQM34_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1450944.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30310..32664
                     /gene="lptD"
                     /locus_tag="OQM34_000368"
     CDS             30310..32664
                     /gene="lptD"
                     /locus_tag="OQM34_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1450945.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32717..34003
                     /gene="surA"
                     /locus_tag="OQM34_000369"
     CDS             32717..34003
                     /gene="surA"
                     /locus_tag="OQM34_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1450946.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34003..34992
                     /gene="pdxA"
                     /locus_tag="OQM34_000370"
     CDS             34003..34992
                     /gene="pdxA"
                     /locus_tag="OQM34_000370"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1450947.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            34989..35810
                     /gene="rsmA"
                     /locus_tag="OQM34_000371"
     CDS             34989..35810
                     /gene="rsmA"
                     /locus_tag="OQM34_000371"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1450948.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            35813..36190
                     /gene="apaG"
                     /locus_tag="OQM34_000372"
     CDS             35813..36190
                     /gene="apaG"
                     /locus_tag="OQM34_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1450949.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36197..37039
                     /gene="apaH"
                     /locus_tag="OQM34_000373"
     CDS             36197..37039
                     /gene="apaH"
                     /locus_tag="OQM34_000373"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1450950.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37117..37596)
                     /gene="folA"
                     /locus_tag="OQM34_000374"
     CDS             complement(37117..37596)
                     /gene="folA"
                     /locus_tag="OQM34_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1450951.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37787..39649)
                     /gene="kefC"
                     /locus_tag="OQM34_000375"
     CDS             complement(37787..39649)
                     /gene="kefC"
                     /locus_tag="OQM34_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1450952.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39642..40172)
                     /gene="kefF"
                     /locus_tag="OQM34_000376"
     CDS             complement(39642..40172)
                     /gene="kefF"
                     /locus_tag="OQM34_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1450953.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40220..41614)
                     /locus_tag="OQM34_000377"
     CDS             complement(40220..41614)
                     /locus_tag="OQM34_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1450954.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41673..41960)
                     /gene="fixX"
                     /locus_tag="OQM34_000378"
     CDS             complement(41673..41960)
                     /gene="fixX"
                     /locus_tag="OQM34_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCS1450955.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(41957..43243)
                     /locus_tag="OQM34_000379"
     CDS             complement(41957..43243)
                     /locus_tag="OQM34_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1450956.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43294..44235)
                     /locus_tag="OQM34_000380"
                     /pseudo
     CDS             complement(43294..44235)
                     /locus_tag="OQM34_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44250..45020)
                     /locus_tag="OQM34_000381"
     CDS             complement(44250..45020)
                     /locus_tag="OQM34_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCS1450957.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTFTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45492..47006
                     /gene="caiT"
                     /locus_tag="OQM34_000382"
     CDS             45492..47006
                     /gene="caiT"
                     /locus_tag="OQM34_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCS1450958.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47037..48179
                     /gene="caiA"
                     /locus_tag="OQM34_000383"
     CDS             47037..48179
                     /gene="caiA"
                     /locus_tag="OQM34_000383"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCS1450959.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48308..49525
                     /gene="caiB"
                     /locus_tag="OQM34_000384"
     CDS             48308..49525
                     /gene="caiB"
                     /locus_tag="OQM34_000384"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCS1450960.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49599..51152
                     /gene="caiC"
                     /locus_tag="OQM34_000385"
     CDS             49599..51152
                     /gene="caiC"
                     /locus_tag="OQM34_000385"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCS1450961.1"
                     /translation="MDIIGGQHLRQIWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51261..52046
                     /gene="caiD"
                     /locus_tag="OQM34_000386"
     CDS             51261..52046
                     /gene="caiD"
                     /locus_tag="OQM34_000386"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCS1450962.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSTGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52052..52642
                     /gene="caiE"
                     /locus_tag="OQM34_000387"
     CDS             52052..52642
                     /gene="caiE"
                     /locus_tag="OQM34_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCS1450963.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52728..53123)
                     /gene="caiF"
                     /locus_tag="OQM34_000388"
     CDS             complement(52728..53123)
                     /gene="caiF"
                     /locus_tag="OQM34_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCS1450964.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53385..56606)
                     /gene="carB"
                     /locus_tag="OQM34_000389"
     CDS             complement(53385..56606)
                     /gene="carB"
                     /locus_tag="OQM34_000389"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1450965.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLALSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56624..57772)
                     /gene="carA"
                     /locus_tag="OQM34_000390"
     CDS             complement(56624..57772)
                     /gene="carA"
                     /locus_tag="OQM34_000390"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1450966.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58228..59049)
                     /gene="dapB"
                     /locus_tag="OQM34_000391"
     CDS             complement(58228..59049)
                     /gene="dapB"
                     /locus_tag="OQM34_000391"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1450967.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59216..60130)
                     /gene="rihC"
                     /locus_tag="OQM34_000392"
     CDS             complement(59216..60130)
                     /gene="rihC"
                     /locus_tag="OQM34_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1450968.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60196..61146)
                     /gene="ispH"
                     /locus_tag="OQM34_000393"
                     /gene_synonym="lytB"
     CDS             complement(60196..61146)
                     /gene="ispH"
                     /locus_tag="OQM34_000393"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1450969.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61148..61597)
                     /gene="fkpB"
                     /locus_tag="OQM34_000394"
     CDS             complement(61148..61597)
                     /gene="fkpB"
                     /locus_tag="OQM34_000394"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1450970.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61684..62178)
                     /gene="lspA"
                     /locus_tag="OQM34_000395"
     CDS             complement(61684..62178)
                     /gene="lspA"
                     /locus_tag="OQM34_000395"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1450971.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62178..64994)
                     /gene="ileS"
                     /locus_tag="OQM34_000396"
     CDS             complement(62178..64994)
                     /gene="ileS"
                     /locus_tag="OQM34_000396"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1450972.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65037..65978)
                     /gene="ribF"
                     /locus_tag="OQM34_000397"
     CDS             complement(65037..65978)
                     /gene="ribF"
                     /locus_tag="OQM34_000397"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS1450973.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(65986..66204)
                     /locus_tag="OQM34_000398"
     CDS             complement(65986..66204)
                     /locus_tag="OQM34_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1450974.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66307..66570
                     /gene="rpsT"
                     /locus_tag="OQM34_000399"
     CDS             66307..66570
                     /gene="rpsT"
                     /locus_tag="OQM34_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1450975.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16232 a        18199 c        17222 g        15247 t
ORIGIN      
        1 gatgtccagt cccgtactct acgcgccaga gggaaattca cctggcgcgt atttttgttc
       61 gcggattagc tacggctgag cacgccgcgg cgatagagat tacgtttaat gcgcgttaaa
      121 ccaggttttg gcttacgcgg ttcgtccagg cttgccagga caatctccag cacacgctct
      181 gcgacatcgc ggtgacgttg agccactgcc agcaccggac actgtaagaa gtcgagcagt
      241 tcgttatcgc caaaggtggc aattgccagg tcagaaggca gtttgccgtc gcgacgcagc
      301 gtgacatcca tcactccttg caacaacgca aacgacgttg tgaacagcgc ctgcggcatc
      361 ggatgcgttt ccagccattt ttcgaataac tgggcagccg cctcccgctc atagctgttg
      421 gcatacagga aatgcacttc gcgcggatca tctttccaag cagtacggaa accttgttca
      481 cgcaggaagc tgacagaaag ctccggcagc gcgccaagat aaagcaccgt ctcggcggga
      541 aacttacgta actcttccgc cagcatttcg gcatcatcct gatcggcacc aaccacgctg
      601 gtgaaatgtt cacgatcgag ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc
      661 cagcgttgat aaaaaggatg ctcaggtggc aacgacgtcg aaacaataat ggcatcaacc
      721 tgacgctgta aaaggtgctc aatgcagcgc atttcgttgt ctggctgatc ttctgagcag
      781 gcaatcagca gttgataacc ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg
      841 gtatagctgg tgttctccag atcggggatc acaagaccaa tagaacgtgt gcgtccagca
      901 cgaagcccag ctgccacggc gttcgggtgg taattgtgct cacgcaccac agccatgact
      961 ttttcaacgg ttttgtcgct cacacggtat tgcttcgctt tgccgttaat aacatagctt
     1021 gcagtggtcc gcgacactcc cgccagccga gcgatttcat ccagtttcac aattgcccct
     1081 tgcgtaaaat gtaaaaaacc atagccattg tactggtatg ggttaaattc attaacatct
     1141 aagcgcagaa aattaactgc ggcaactgct tttattccag gttcccactg atttcgcaaa
     1201 aaaagcccaa cgtgacatgt tgggctgcaa attgcgcact gagatcattc tcaacgcatt
     1261 attttatcgc cgcgcgaaag tccgaccaca ccagagcgag caacttccac aattttcgcc
     1321 acatcgcgaa tcgatgctaa aaatgcatca agcttatcgc tggtgcctgc taattgaacg
     1381 gtataaagcg agggggtgac atcgataatt tgcccacgga atatttccgt attacgtttc
     1441 acttcgtcac gcccgtaacc gctggcctga attttcacca gcatgatttc ccgctcaaca
     1501 tgcgcgccct gccccaactc actcacgcgc aagacatcga ccagcttgtg taattgcttt
     1561 tcgatctgct caagtacttt ttcatcgccc acggtctgga tggtcatacg cgataatgtc
     1621 ggatcgtcgg ttggcgcaac ggtcaggctt tcaatgttgt agcctcgctg ggaaaaaagg
     1681 ccaatcacgc gggataacgc gcctgattca ttttcgagta agactgataa tatccggcgc
     1741 ataatcaggt tctctccgtt ttgcttaacc acatttcatc cattccgccc ccgcgaatct
     1801 gcatcgggta gacgtgctca ctgccatcga cggtaacatc aacaaacacc aggcgattat
     1861 tgcgcacctg ttccagcgcc tcgctaagtt tgctttccag ctcatgcgga tgagagatct
     1921 ggatcccgac gtgaccatag gcttccgcca gacggacgaa atcgggtagt gattgcatat
     1981 aagattgtga atgacggccg gaatagatca tgtcctgcca ctgtttcacc atccccagat
     2041 agcggttatt gagattcacc accagtacgg gcaactcgta ttgcaacgcg gtagacagtt
     2101 cctggatgtt catctgaata ctgccgtcgc cagtgacgca aaccacggtt tcttctggca
     2161 acgccatttt gacgcccagt gccgcaggta aaccaaaacc catcgtgccg agaccgccgg
     2221 aattgatcca gcgacgcggt ttgtcgaatg gataataaag cgcagcaaac atctggtgct
     2281 gcccgacatc ggacgtcacg taagcgtccc cattagtcaa ccgccaaaga gtctcgatca
     2341 ccgcctgcgg tttaatcttt tcactgtgag tgtcatattt caggcactga agagcgcgcc
     2401 actgttcaat ctgctgccac cagtcgcgga tctcatccag cggttgatgg gcggattctt
     2461 gcgacaggag ttcaagcatt tgttcgagga cctggcgagc atcccccaca atcgggatat
     2521 ccgcagtcac ggttttagaa atggaagtag gatcaatatc gatatgcaga acagtggcat
     2581 ttgggcagta ctttgccaga ttgttcgtcg ttcggtcatc aaatcgtacc ccgacggcga
     2641 aaatcacatc cgcgttatgc atcgtcatat tggcttcgta ggtaccgtgc attcccagca
     2701 tgcccagtgc ctgacgatgc gttgccggaa acgcccccag ccccatcaat gaggaaacaa
     2761 cgggcagatt caacgcctcc accgtttctt tcaactgctg atggcagccc gccatgattg
     2821 ccccaccgcc tacgtagaca accggttttt ttgccgctac cagcgtttgc agagcacgct
     2881 taatttgccc tttatgtccg gtagtagtgg gattgtaaga acgcatactg accgactcag
     2941 ggcagacata gggtaatttg ttcgccggat taagaatatc tttcggtaaa tcaacgacca
     3001 ctggtcctgg gcgaccactt gccgccagcc agaaagcctt tttcagcacc tgcggaatgt
     3061 cttccgtttg cttaaccaga aaactgtgtt taaccaccgg tcgcgaaatc cccaccatgt
     3121 cgcactcctg aaaggcatcg taacctatca acgaggtcgc tacctgcccg gaaaggacaa
     3181 ctaatggaat ggaatccata taagcggtgg cgatgccggt aatcgcattg gtcgcccctg
     3241 gacccgacgt taccagcacg acgccgactt ccccggtcgc acgcgccagg ccatcggcca
     3301 tatgcaccgc cgcctgctca tgacgaacta atacatgatc aataccaccc acggtatgca
     3361 atgcatcgta aatatcaagg actgcgcctc cgggataacc gaatacttgt ttaacgccct
     3421 gatcgataag cgatcggacg accatctcgg ctccagacaa catctccatg gcctgcctca
     3481 ctgtttgacg gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt aagcaattgg
     3541 cagccagaga cataaacgca gggtaataag aataaacagg aggaaaggtg aaaagaaacg
     3601 ataaaatcct ccattacgaa ggattaaatt gataataaat cggctgaatc ccacaactta
     3661 cagcaatttc tctatttttt taattaatcc taaaatagag agtccacaca ttcagaataa
     3721 aattctacat tcaataaaaa atgcagaata aaccagacat tcatgtctgg cctattctgc
     3781 attcagttag cgtttgcaaa ttgagactaa ttgttcttcc atccactgat ggcctttatc
     3841 gcgcccggca gcttcatgcc aagagagata acaggttctg ctgttttgtt ttaacggcag
     3901 cggtaatacc tgtaattcta aggattcagc gaactcttca gccagccaac gcggcgcaat
     3961 agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata
     4021 cgcgatactg gcttgcttat ctaccgtgtc ataccaaggt tgactaaatg acgcgaaacg
     4081 atcgagcgaa accgccgcat gttgttcgtt ataaacatca tgtttcagta acgggccttt
     4141 aattgttgga tgatttttgc tggctaccag caccatttca tctttaaata atggcacgct
     4201 ggtaaattca ggacgatgga agtcttcata actaatcaca aactccgttt cctgataacg
     4261 cagctgatgt tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc
     4321 aatctgctca atgtgattat aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac
     4381 acaaagatga aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt
     4441 ttgtactagt tgcaatgcct ggcgaactga accaaaaagt tgaaatgcgc gagcagtcgg
     4501 ttgaatacca cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc
     4561 aacagcgtta ctgaccgcag gttgcgacat acccagaaca tgagcggcac gggtaatatt
     4621 ttgctcctgc atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg
     4681 tggcttgctt aactccgccg tctctgaatg atctgtttgt acctctggca tacttaactc
     4741 cactgtcaca cttaactccc tttcccttat tggaatgcag agaaataatc ctgaagaata
     4801 tgatttatca tgcatataaa ataagaaaaa gcaaaatgag taaaattcgg aaaacataaa
     4861 gatacgggtt ctgataaaaa cagaatccat tgccgtggaa tgagtcattt accacagcat
     4921 aataatccat aatgtttttg ttgcgaaaac aatctaatca taactaccgc gaatactcaa
     4981 tcatctacaa aatggattaa atgtgcttat ttaataatta atttatatat ttaatgcatt
     5041 aattcttaac attaattgat caataatatt caccaaatca atatcaaaaa aaatcgcaaa
     5101 acatataatt caatacaaat catcaggata ggttttgcaa cgcgtgcatt ttgtcccctt
     5161 tttcctcgtt gattagatgc aaaaatttat gctgaaatat gtcaaccgat gaaaagcgtc
     5221 ggtagttaag cagaaattaa tatcgcttac tttaaccacc gtagcacaat tagctaattt
     5281 tacggatgca gaactcacgc tggcgggacg tttttattgc gtcagggttg acatccgttt
     5341 ttgtatccag taactctaaa agcatatcgc attcatctgg agctgattta atgactcaca
     5401 tcattcgctt tatcggtcta ctactactaa acgcatcttc tttgcgcggt agacgagtga
     5461 gcggcatcca gcattaagcc agcacgcagt caaacaaaaa acccgcgcca ttgcgcgggt
     5521 ttttttatgc ccgaagcgag gcgctctaaa agagacaagg acccaaacca tgagccagca
     5581 agtcattatt ttcgatacca cattgcgcga cggtgaacag gcgttacagg caagcttgag
     5641 tgtgaaagaa aaactgcaaa ttgcgctggc ccttgagcgt atgggtgttg acgtgatgga
     5701 agtcggtttc cccgtctctt cgccgggcga ttttgaatcg gtgcaaacca tcgcccgcca
     5761 ggttaaaaac agccgcgtat gtgcgttagc tcgctgcgtg gaaaaagata tcgacgtggc
     5821 ggccgaatcc ctgaaagtcg ccgaagcctt ccgtattcat acctttattg ctacctcccc
     5881 aatgcacatc gccaccaagc tgcgcagcac cctagacgag gtgatcgaac gcgctatcta
     5941 tatggtgaaa cgcgcccgta attacaccga tgatgttgaa ttttcttgcg aagatgccgg
     6001 gcgtacaccc attgccgatc tggcgcgagt ggtcgaagcg gcgattaatg ccggtgccac
     6061 caccatcaac attccggaca ccgtgggcta caccatgccg tttgagttcg ccggaatcat
     6121 cagcggcctg tatgaacgcg tacctaacat cgacaaagcc attatttccg tacataccca
     6181 cgacgattta ggcctggcgg tcggcaactc actggcggcg gtacatgccg gtgcacgcca
     6241 ggtggaaggt gcaatgaacg ggatcggcga gcgtgccggt aactgttcgc tggaagaagt
     6301 catcatggcg attaaagttc gtaaggatat tctcaacgtc cacaccgcca ttaatcacca
     6361 agagatatgg cgcaccagcc agttagttag ccagatttgt aatatgccga tcccggcaaa
     6421 caaagccatt gttggcagcg gcgcattcgc tcactcctcc ggtatccacc aggatggcgt
     6481 gctgaaaaac cgcgaaaact acgaaatcat gacaccagaa tctattggtc tgaaccaaat
     6541 ccagctgaat ctgacctctc gttcggggcg tgcggcggtg aaacatcgca tggatgagat
     6601 ggggtataaa gaaagtgaat ataatttaga caatttgtac gatgctttcc tgaagctggc
     6661 ggacaaaaaa ggtcaggtgt ttgattacga tctggaggcg ctggccttca tcggtaagca
     6721 gcaagaagag ccggagcatt tccgtctgga ttacttcagc gtgcagtctg gctctaacga
     6781 tatcgccacc gccgccgtca aactggcctg tggcgaagaa gtcaaagcag aagccgccaa
     6841 cggtaacggt ccggtcgatg ccgtctatca ggcaattaac cgcatcactg actataacgt
     6901 cgaactggtg aaatacagcc tgaccgccaa aggtcacggt aaagatgcgc tgggtcaggt
     6961 ggatattgtc accaactaca acggtcgccg cttccacggc gtcggcctgg ccaccgatat
     7021 tgtcgagtcc tccgccaaag ccatggtgca cgtactgaac aatatctggc gtgccgcaga
     7081 agtcgaaaaa gagttgcaac gcaaaactca acacaacgaa aacaacaagg aaaccgtgtg
     7141 atgtcgaaga attaccatat tgccgtattg ccgggagacg gtattggtcc ggaagtgatg
     7201 acccaggcgc tgaaagtgct gaatgccgtg cgcaaccgct ttgcgatgcg catcaccacc
     7261 agccattacg atgtaggcgg cgcagccatt gataaccacg ggcaaccact gccgcctgca
     7321 acggttgaag gttgtgagca agccgatgcc gtgctgtttg gctcggtagg cggcccgaag
     7381 tgggaacatt taccaccaga ccagcaacca gaacgcggcg cgctgctgcc gctgcgtaag
     7441 cacttcaaat tattcagcaa cctgcgcccg gcaaaactgt atcaggggct ggaagcattc
     7501 tgtccgctgc gtgcagacat tgccgctaac ggcttcgaca tccagtgcgt gcgcgaactg
     7561 accggcggca tctatttcgg tcagccaaaa ggccgcgaag gtagcggaca atatgaaaaa
     7621 gcgtttgata ccgaggtgta tcaccgtttt gagatcgagc gtattgcccg catcgcgttt
     7681 gaatctgctc gtaaacgtcg ccacaaagtc acctcaatcg acaaagctaa cgtgctgcaa
     7741 tcctctattt tatggcgtga aatcgtcaac gagatcgcca cggaataccc ggatgtcgaa
     7801 ctggcgcata tgtacatcga caacgccacc atgcagctga ttaaagatcc atcacagttt
     7861 gacgttctgc tgtgctccaa cctgtttggc gacattctgt ctgacgagtg cgcaatgatc
     7921 actggctcga tgggcatgtt gccttccgcc agcctgaacg agcaaggttt tggactgtat
     7981 gaaccggcgg gcggctcggc accagatatc gcaggcaaaa acatcgccaa cccgattgca
     8041 caaatcctgt cgctggcact gctgctgcgt tacagcctgg atgccgatga tgcggcttcc
     8101 gccattgaac gcgccattaa ccgtgcatta gaagaaggca ttcgcaccgg ggatttagcc
     8161 cgtggcgctg ccgccgttag taccgatgaa atgggcgata tcattgcccg ctatgtggca
     8221 gaaggggtgt aatcatggct aaaacgttat acgaaaaatt attcgacgct cacgtagtgt
     8281 acgaagccga aaacgaaacc ccgctgttat atatcgaccg ccacctggtg catgaagtga
     8341 cctcaccgca ggcgttcgat ggcctgcgcg cccacggtcg cccggtacgt cagccgggca
     8401 aaaccttcgc cactatggat cacaacgtct ctacccagac caaagacatt aatgcctgtg
     8461 gtgaaatggc gcgcatccag atgcaggagc tgattaaaaa ctgcaaagaa tttggcgtcg
     8521 agctgtatga cctgaatcac ccgtatcagg ggatcgtcca cgtaatgggg ccggaacagg
     8581 gcgtcacctt gccggggatg accattgtct gcggcgactc gcataccgcc acccacggcg
     8641 cgtttggcgc actggccttt ggtatcggca cttccgaagt tgaacacgta ctggcaacac
     8701 aaaccctgaa acagggccgt gcgaagacca tgaaaattga agtccagggc aaagccgcgc
     8761 cgggcattac cgcaaaagat atcgtgctgg caattatcgg taaaactggc agcgcaggcg
     8821 gcaccgggca tgtggtggag ttttgcggcg aagcaattcg tgatttaagc atggaaggtc
     8881 gtatgaccct gtgcaatatg gcgatcgaaa tgggcgcaaa agccggtctg gttgctcctg
     8941 acgaaaccac ctttaactat gtcaaaggcc gtctgcatgc gccgaaaggc aaagatttcg
     9001 acgacgccgt tgcctactgg aaaaccctgc aaaccgacga aggcgcaact ttcgataccg
     9061 ttgtcactct gcaagcagaa gaaatttcac cgcaggtcac ctggggcacc aatcccggcc
     9121 aggtgatttc tgtgaacgac aatattcccg atccggcttc gtttgccgat ccggttgaac
     9181 gtgcgtcggc agaaaaagcg ctggcctata tggggctgaa accgggtatt ccgctgaccg
     9241 aagtggctat cgacaaagtg tttatcggtt cctgtaccaa ctcgcgcatt gaagatttac
     9301 gcgcggcagc ggagatcgcc aaagggcgaa aagtcgcgcc aggcgtgcag gcactggtgg
     9361 ttcccggctc tggcccggta aaagcccagg cggaagcgga aggtctggat aaaatattta
     9421 ttgaagccgg ttttgaatgg cgcttgcctg gctgctcaat gtgtctggcg atgaacaacg
     9481 accgtctgaa tccgggcgaa cgttgtgcat ccaccagcaa ccgtaacttt gaaggccgcc
     9541 aggggcgcgg cgggcgcacg catctggtca gcccggcaat ggctgccgct gctgctgtga
     9601 ccggacattt cgccgacatt cgcaacatta aataaggagc acaccatggc agagaaattt
     9661 atcaaacaca caggcctggt ggttccgctg gatgccgcca atgtcgatac cgatgcaatc
     9721 atcccgaaac agtttttgca gaaagtgacc cgtacgggtt ttggcgcgca tctgtttaac
     9781 gactggcgtt ttctcgatga aaaaggccaa cagccaaacc cggacttcgt gctgaacttc
     9841 ccgcagtatc agggcgcttc cattttgctg gcacgagaaa acttcggctg tggctcctcg
     9901 cgtgagcacg cgccctgggc attgaccgac tacggtttta aagtggtgat tgcgccgagt
     9961 tttgctgaca tcttctacgg caatagcttt aacaaccagc tgctgccggt gaaattaagc
    10021 gatgcagaag tggacgaact gtttgcgctg gtgaaagcca atccggggat ccatttcgac
    10081 gtggatctgg aagcgcaaga ggtgaaagcg ggagagaaaa cctatcgctt taccatcgat
    10141 gccttccgcc gccactgcat gatgaatggt ctggacagca tcggcctgac cctgcagcac
    10201 gacgacgcta ttgcgtctta tgaagcaaaa caacctgcgt ttatgaatta gaccccttgc
    10261 ccggtcaaat gaccgggctt tccgctatcg tccacgtcat caaacgtctt taacctttgc
    10321 ggttaaaaat aatgcgacaa cagaaataat cgcaattacc cagtagacag caaagtgccc
    10381 ccaactttgt gcaattgctc cctgaataac accagccaga attaccccgg tagagatagt
    10441 gttggtaaat aaggtggtag ctgcccccgc tcttccaggc attaaatcct gaaaccatag
    10501 catcccaata cccgcaacaa tgccgataaa tacagcgtta aaaagttgca gcgtcatcaa
    10561 cgccatacgg ctatgaaaga aaatcaatcc agtgtaaaac agcactcctg ccaccactgc
    10621 aatgaccatc attcgccgct taccataacg tttgacatag tagccagcca gaatcattgc
    10681 tggtatttcc agtccagctg ccgtccccat caggaaaccc gccagtttgt ctggcaatcc
    10741 taaatcgcta ctgatccaca atggcatatc aataatgtac atagtgttgc aggtccacat
    10801 taacgtcgag gcgacaaata acatccgtac gttactatcc tgccagtcac cttgcattga
    10861 taaagcattt tccgacggca gttctacccg cgccagagca aataactgtg gcattgccgt
    10921 attggccaga gatgccagaa gcacaccaca ggtgataagc gtcagataat gacgattaaa
    10981 tgcaaacagt agcgcattgc caatagccat caaacagcaa aatataatca gttttcgccg
    11041 atcgccctga ctgtcagaac gttttgccaa ccagaggctt acgccgatcc cagcaatagc
    11101 attcaccgta taaaagagtc cgatccagaa aggttgcgcg ccaacctcac gactcagaaa
    11161 taagctcaat gtaggagcct gtagcgcccc ggccaccccc atcataaaag cgaccagcat
    11221 aaatgccgcg taaacaccgt tcatacggcg agccatcgtc attatccaga tcatacgttc
    11281 cctttttagc gcggcgagaa taaaaaaaac cagcaggtat aatctgctgg cgggtgattt
    11341 tacaccaata ctcagtcaca catgatgcag gcaagtcaac tttcagaatt gcggtcatcc
    11401 cactgcatca gtccttcaag catggttaat cgttgtggga cgcttaacca acgcaaccag
    11461 cacaacttcg ctgtcgcggt aaaatagtgc tgataaaact gacgcatggg gcacctcctt
    11521 gcttcatcgt taggaattat tgacttaata tagggaaaat aaaattgctg tcttttgcgc
    11581 aggagttccc cttttatgcc atctgctcgt ctgcaacaac agttcatccg cctgtggcaa
    11641 tgctgcgagg gtaaatcgca ggacacaacg ctcaacgaac tggcagcgtt attgagctgc
    11701 tcgcgtcgtc atatgcgtac cctgctcaac accatgcagg atcgcggctg gctgacgtgg
    11761 gaagcggaag tcgggcgcgg taaacgctcg cgtctgacat tcctctatac cgggctggcg
    11821 cttcagcaac agcgggcgga agacctgctg gagcaggatc gtatcgacca actagtgcag
    11881 ttggttggcg acaaagcgac tgtgcggcaa atgctggttt ctcatctggg ccgcagcttc
    11941 cgccaggggc ggcacatcct gcgcgtgctc tactatcgtc cgttgcgtaa tctgctacct
    12001 ggcagcgcat tgcgccgttc cgaaacccat attgcccggc aaatcttcag ttcgctaacg
    12061 cgcataaatg aggaaaatgg ggaactggaa gcagacatcg cccaccactg gcagcaaata
    12121 tcaccgcttc actggcgttt ctttttgcgt ccaggagtcc attttcatca tggtcgtgaa
    12181 ctggaaatgg acgacgtgat cgcctcttta aaacgaatca atacgctgcc gctctattcg
    12241 catattacgg acattgtgtc gccgacgccc tggacgctgg atatccatct cacgcagccg
    12301 gaccgctggt tgccgttact gctggggcaa gttccggcga tgatcctgcc gtgcgaatgg
    12361 gaaaccctca gtaactttgc cagccatcct attggcaccg gtccgtatgc ggtgattcgc
    12421 aacagcacca atcaactgaa aattcaggca ttcgatgact tcttcggtta ccgggcatta
    12481 atcgacgaag ttaacgtctg ggttctgccg gaaattgccg acgagccagc cggtggtctg
    12541 atgctaaaag gtccacaggg cgagaaaaaa gagattgaaa gccgcctgga ggaaggttgc
    12601 tactatttac tgttcgacag ccgcacccat cgcggggcga atcagcaagt cagggactgg
    12661 gtaagctatg tgctttctcc aactaatctg gtctatttcg ctgaggaaca gtaccagcaa
    12721 ctgtggttcc cggcttatgg actgctcccc cgttggcacc atgcccgcac cataaagagc
    12781 gaaaaaccgg ctggcctgga aagcctcacc ctgacctttt atcaggatca cagtgagcat
    12841 cgggtgattg ccgggatcat gcagcagatt ctggcaagtc accaggtcac gctggaaatc
    12901 aaagagatca gctacgatca gtggcatgaa ggagagatcg agagcgatat ctggcttaat
    12961 agcgccaact ttacgctgcc gctggatttt tcgctgttcg cgcacttgtg cgaggtgccg
    13021 ctgctccaac actgtattcc gatcgactgg caagccgacg ccgcccgctg gcgcaatggc
    13081 gaaatgaacc tggcgaactg gtgccagcaa ctggtcgcca gtaaagcaat ggtgccgctt
    13141 atccaccact ggctgatcat tcagggacaa cgcagtatgc gcggcctgcg tatgaatacc
    13201 ctcggctggt tcgattttaa atcagcgtgg tttgcgccac cggatccatg attgctggta
    13261 gataacaaaa tcactacact aacgccgttc tcaacggggt gccacgcaaa cgcgtgcgct
    13321 gagaaaatac ccgtcgaacc tgatccggat aacgccggcg aagggatttg aggctccttc
    13381 tcaagtcctt tgccactctt ttttgaggtg caaagtgtta aaaaaatgtc ttcccctgct
    13441 gttgctgtgc acagcgcccg ttttcgctaa acccgttctg actgtttata cctacgattc
    13501 cttcgccgcc gactgggggc ctggtccaaa gatcaaaaaa gcttttgaag ccgactgtaa
    13561 ctgcgaactg aaactggtgg cgctggaaga tggcgtttcg cttctcaacc gtctacggat
    13621 ggaaggcaaa aatagtaaag ccgatgtggt gctggggctg gataacaatc tgttagacgc
    13681 cgccagtaaa accggactat ttgccaaaag cggtgtggca gcggatgccg ttaacgttcc
    13741 cggcggctgg aataatgaca ctttcgtccc gtttgattac ggctatttcg ccttcgtcta
    13801 tgacaagaac aaactgaaaa acccgccgca aagcctgaaa gaattggttg agagcgatca
    13861 gaactggcgg gtgatttatc aggatccgcg taccagcacg ccggggctgg gtctgctgtt
    13921 gtggatgcaa aaagtctatg gcgatgacgc cccacaagcc tggcaaaaac tggcgaagaa
    13981 aaccgtcacg gtcaccaaag gctggagcga agcctacggc ctgtttttaa aaggtgaaag
    14041 cgatctggta ctgagttaca ccacctctcc ggcttatcac attctcgaag agaaaaaaga
    14101 taactacgcc gccgcgaact ttagcgaagg tcactatctg caggtggaag tcgccgcccg
    14161 caccgctgcc agcaagcagc cggagctggc gcaaaaattc ctccagttta tggtttctcc
    14221 ggcttttcag aatgcgatcc caaccggtaa ctggatgtat ccggtggcaa atgtcacgct
    14281 gcctgccggg tttgaacaat tgaccaaacc ggccaccacg ctggagttca caccagctga
    14341 agtggcggca caacgtcagg catggattag cgaatggcaa cgcgccgtca gccgttaatt
    14401 cccggctggt taattccagg tgtaagcgcc gccacgctgg tggtagcggt tgcgctcgcg
    14461 gcgtttctcg ccctgtggtg gaacgcgccg cagggtaact gggtggcagt ctggcaggac
    14521 agctacctgt ggcatgtggt gcgcttctcc ttctggcagg cgtttctctc ggcgctactc
    14581 tctgtcgtac ccgcgatatt cctcgcccgc gccctctatc gcaggcgctt tccgggtcgg
    14641 ctggcgctgt tgcgtctgtg cgcaatgacc ttgatcctcc cggtgctagt tgctgttttc
    14701 ggcattctta gcgtctatgg tcgtcagggc tggctggcat cgctctgcca atcgctcggt
    14761 ctggagtgga ccttttcgcc ctacggcctg caaggcattt tgctggcgca cgtatttttt
    14821 aatctgccga tggcgagccg cttattactc caggcactgg aaaacattcc cggcgaacaa
    14881 cgtcagcttg ccgcccagct tgggatacgc ggctggcatt ttttccgctt cgtcgaatgg
    14941 ccgtggctac ggcgacaaat cccgccggtt gccgcgctta tctttatgct ctgtttcgcc
    15001 agcttcgcca ccgtgctatc gctgggcggc ggtccgcagg caaccactat cgagctggct
    15061 atttatcagg cgctgagtta cgactacgat cctgcccgcg cggcgatgct ggcgctgctc
    15121 cagatggtgt gttgccttgg gctggtgctg ttgagtcagc gactaagtaa ggccattgcg
    15181 cccggcacca cgctgctgca aggctggcgc gatccggacg atcgtctgca tagccgcatt
    15241 tgcgacacgg tgttaatagt gctggcgctg ctgctgttgc tgccaccgtt gctggcggtg
    15301 atcgtcgatg gggtaaatcg ccagttgccg gaagtgctgg cacaaccggt gctgtggcag
    15361 gcgctgtgga cctcgttgcg tattgcgctg gcggcaggtg tattgtgcgt agtgctgacc
    15421 atgatgctgc tatggagcag tcgcgaactg cgggcgcggc agaaaatgct ggcgggtcag
    15481 gcgctggaga tgagcggcat gttgatcctc gccatgccgg ggattgtgct ggctaccggc
    15541 ttctttttac tgctcaacaa cactatcggc ctgccacaat ctgctgacgg cattgtgatt
    15601 ttcaccaatg cgttaatggc gatcccttat gcgctgaaag tactggaaaa cccgatgcgc
    15661 gatatcaccg cccgctacag tatgttgtgt cagtcgctgg gcattgaagg ctggtcgcgc
    15721 ttaaaagtgg tcgagctgcg cgccctgaaa cgtccactgg cgcaggcgct ggcttttgcc
    15781 tgcgtgctgt cgattggtga ttttggcgta gtggcgttgt tcggtaacga tgatttccgt
    15841 accctgccgt tttatctcta ccagcaaatt ggctcctatc gcagccagga cggcgcggtc
    15901 accgcgttaa ttctgctact actctgtttt ctgctgttta ccgtgattga aaaactaccg
    15961 gggcgaaatg ttaaaactga ctgatatcac ctggctttat caccatttgc cgatgcgttt
    16021 tagcttaacg gtagaacgcg gcgagcaggt ggcaatcctc gggccaagcg gcgcgggtaa
    16081 aagtaccctg ctgaatttga ttgccggttt tctgacgcca gccagcggtt cgctgactat
    16141 cgatggtgtt gatcacacca cgacgccgcc gtcacgccgt ccggtgtcga tgctgtttca
    16201 ggagaacaac ctgttcagcc atctgacggt cgcgcagaac atcgggctgg ggctaaatcc
    16261 ggggttgaaa ctgaacgcgg cacagcagga gaaaatgcac gcgattgccc gtcagatggg
    16321 gattgataat ttaatggcgc ggttaccggg cgagctttcc ggcggtcagc gacagcgcgt
    16381 ggcgttagcg cgttgtctgg tgcgcgaaca gccgatttta ttgctcgatg aaccgttctc
    16441 tgcgctcgat ccggcgttac gtcaggagat gctgacgctg gtgagcacga gctgccagca
    16501 gcaaaaaatg acgctattga tggtgtcgca cagtgtggaa gatgcggcgc ggatcgccac
    16561 acgctcggtg gtggtcgccg acgggcgcat cgcctggcag ggtaagactg aagaattgtt
    16621 gagcggtaag gcgagtgctt cggcaatatt ggggattacg ggttagtatg cactgtcaga
    16681 tgcggcgtga acaccttatc cgacctacgg acagtgcgtt ttgtaggcct gataagacgc
    16741 ggtcagcgtc gcatgaggca acccgcacaa gacaatcccc taaaccccaa ccactttacg
    16801 caaaatatcg atatacaccg gcatcaacgg gtggcgaatt aacaccacca gcgccaccac
    16861 gccgatggca gaaatcaacg gcgtcagcca caacaaacgg ccgcgcgaca aataatgact
    16921 taaacgatcg gttgctttac cgctgcgcca taaccgccag cacagccagc caccaaccca
    16981 caaaaatacc gccgtcgcca gcagcaacca tttaaactcg ccgctctgca ttccggctgg
    17041 aatatcgatc gccgcgcccg ccagaatccc aggcaggaag taaaacggtg gccacagcag
    17101 gcagccgata atattcggcg taatgaattt tgccaccggc agatccagca ttcccgccac
    17161 cattggcacc agcggacgcg tcgggccaac aaagcgaccg accagaatgg tgaacatgct
    17221 gtgttgatgc aacgcatgtt cagttttatc gagtaatgct ttgtttttct tcagaaatga
    17281 ccagcgatgc aacggctttt taaaacgcca gcccagccag aaggagatcc agtcgcccag
    17341 cagacaaccg acaatccctg ccagccaagc gtgccagaaa cttaactcgc cgctgccaat
    17401 cagcgctccc agccccgcca tcagcaccgt accgggtaga atcaaaccga ccagcgccag
    17461 cgactccaga aaggccacca acaccaccgc catcaatgaa tacacggtag attgggtaat
    17521 aaagtgttcc agcaatgctt gcataatgtg cctgtcaaat ggacgaagca gggattctgc
    17581 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact
    17641 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
    17701 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
    17761 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
    17821 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
    17881 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
    17941 ggtgatcgct gatgtactga caagcctcgc gtacacgatt atccatcggt ggatggagcg
    18001 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
    18061 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
    18121 aatacggctg gtgcgcttca tccgggcgaa agaaacccgt attggcaaat attgacggcc
    18181 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
    18241 cgcgagcctc cggatgacaa ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
    18301 cacccggtcg gcagacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
    18361 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
    18421 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
    18481 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
    18541 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
    18601 taacccaacc ggtaaccccg cttattaaaa gcattctgta acaaagcagg accaaagcca
    18661 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
    18721 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
    18781 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt tggatggagt
    18841 gaaacgatgg cgattgcaat tggcctcgat tttggcagtg attctgtgcg agctttggcg
    18901 gtggactgcg ccagcggtga agagatcgcc accagcgtag agtggtatcc ccgttggcaa
    18961 aaagggcaat tttgtgatgc cccgaataac cagttccgtc atcatccgcg tgactacatt
    19021 gagtcaatgg aagcggcact gaaaaccgtg cttgcagagc ttagcgtcga acagcgcgca
    19081 gctgtggtcg ggattggcgt tgacagtacc ggctcgacgc ccgcaccgat tgatgccgac
    19141 ggtaacgtgc tggcgctgcg cccggagttt gccgaaaacc cgaacgcgat gttcgtattg
    19201 tggaaagacc acactgcggt tgaagaagcg gaagagatta cccgtttgtg ccacgcgccg
    19261 ggcaacgttg actactcccg ctatattggc ggtatttatt ccagcgaatg gttctgggca
    19321 aaaatcctgc atgtgactcg ccaagacagc gccgtggcgc aatctgccgc atcgtggatt
    19381 gagctgtgcg actgggtgcc agctctgctt tccggtacca cccgcccgca ggatattcgt
    19441 cgcggacgtt gcagcgccgg gcataaatct ctgtggcacg aaagctgggg cggcttgccg
    19501 ccagccagtt tctttgatga gctggacccg atcctcaatc gccatttgcc ttccccgctg
    19561 ttcactgaca cctggactgc cgatattccg gtgggcacct tatgcccgga atgggcgcag
    19621 cgtctcggcc tgcctgaaag cgtggtgatt tccggcggcg cgtttgactg ccatatgggc
    19681 gcagttggcg caggcgcaca gcctaacgca ctggtaaaag ttatcggtac ttccacctgc
    19741 gacattctga ttgccgacaa acagagcgtt ggcgagcggg cagttaaagg tatttgcggt
    19801 caggttgatg gcagcatggt gcctggattt atcggtctgg aagcaggcca atcggcgttt
    19861 ggtgatatct acgcctggtt tggtcgcgta ctcggctggc cgctggaaca gcttgccgcc
    19921 cagcatccgg aactgaaagc gcaaatcaac gccagccaga aacaactgct tccggcgctg
    19981 accgaagcat gggccaaaaa tccgtctctg gatcacctgc cggtggtgct cgactggttt
    20041 aacggtcgtc gcacgccaaa cgctaaccaa cgcctgaaag gggtgattac cgatcttaac
    20101 ctcgctaccg acactccgct gctgttcggc ggtttgattg ctgccaccgc ctttggcgca
    20161 cgcgcaatca tggagtgctt taccgatcag gggatcgccg tcaataacgt gatggcgctg
    20221 ggcggcatcg cgcggaaaaa ccaggtcatt atgcaggcct gctgcgacgt gctgaatcgc
    20281 ccgctgcaaa ttgttgcctc tgaccagtgc tgtgcgctcg gtgcggcgat ttttgctgcc
    20341 gtcgccgcga aagtgcacgc agacatccca tcagcccagc aaaaaatggc cagtgcggta
    20401 gagaaaaccc tgcaaccgcg cagcgaacag gcacaacgct ttgaacagct ttatcgccgc
    20461 tatcagcaat gggcgatgag cgccgaacaa cactatcttc caacttccgc cccggcacag
    20521 gctgcccagg ccgttgcgac tctataagga cacgataatg gcgatttttg ataattatga
    20581 agtgtggttt gtcattggca gccagcatct gtatggcccg gaaaccctgc gtcaggtcac
    20641 ccaacatgcc gagcacgttg ttaatgcgct gaatacggaa gcgaaactgc cctacaaact
    20701 ggtgttgaaa ccgctgggca ccacgccgga tgaaatcacc gctatttgcc gcgacgcgaa
    20761 ttacgacgat cgttgcgctg gtctggtggt gtggctgcac accttctccc cggccaaaat
    20821 gtggatcaac ggcctgacca tgctcaacaa accgttgctg caattccaca cccagttcaa
    20881 cgcggcgctg ccgtgggaca gtatcgatat ggactttatg aacctgaacc agactgcaca
    20941 tggcggtcgc gagttcggct tcattggcgc gcgtatgcgt cagcaacatg ccgtcgttac
    21001 cggtcactgg caggataaac aagcccatga gcgtatcggc tcctggatgc gtcaggcggt
    21061 ctctaaacag gatacccgtc atctgaaagt ctgccgattt ggcgataaca tgcgtgaagt
    21121 ggcggtcacc gatggcgata aagttgccgc acagatcaag ttcggtttct ccgtcaatac
    21181 ctgggcggtt ggcgatctgg tgcaggtggt gaactccatc agcgacggcg atgttaacgc
    21241 gctggtcgat gagtacgaaa gctgctacac catgacgcct gccacacaaa tccacggcga
    21301 aaaacgacag aacgtgctgg aagcggcgcg tattgagctg gggatgaagc gtttcctgga
    21361 acaaggtggc ttccacgcgt tcaccaccac ctttgaagat ttgcacggtc tgaaacagct
    21421 tccaggtctg cccgtacagc gtctgatgca gcagggttac ggctttgcgg gcgaaggcga
    21481 ctggaaaacc gccgccctgc ttcgcatcat gaaggtgatg tcaaccggtc tgcagggcgg
    21541 cacctccttt atggaggact acacctatca cttcgagaaa ggtaatgacc tggtgctcgg
    21601 ctcccatatg ctggaagtct gcccgtcgat tgccgtagaa gagaaaccga tcctcgacgt
    21661 tcagcatctc ggtattggtg gtaaggacga tcctgcccga ctgatcttca atacccaaac
    21721 cggtccagcg attgtcgcca gcctgattga tctcggcgat cgttaccgtc tgctggttaa
    21781 ctgtatcgac acggtgaaaa caccgcactc cctgccgaaa ctgccggtgg cgaatgcact
    21841 gtggaaagcg caaccggatc tgccaactgc ttccgaagcg tggatcctcg ctggtggcgc
    21901 gcaccatacc gtcttcagcc atgcgctgaa cctcaacgat atgcgccagt tcgccgagat
    21961 gcacgacatt gaaatcacgg tgattgataa cgacacccgc ctgccagcgt ttaaagacgc
    22021 actgcgctgg aacgaagtgt attacggatt tcgtcgctaa gtagccacat caggtatgta
    22081 acgcctgatg cgacgctgac gcgtcttatc tggcctacac gctgcgattt tgtaggccgg
    22141 ataagcaaag cgcatccggc attcaacgcc tgatgcgacg ctggcgcgtc ttatcaggcc
    22201 tacgcgctgc gattttgtag accggataag cgaagcgcat ccggcacgaa ggagtcaaca
    22261 tgttaaaaga tctcaaacgc caggtattag aagccaacct ggcgctgcca aaacacaacc
    22321 tggtcacgct cacatggggc aacgtcagcg ccgttgatcg cgagcgcggc gtctttgtga
    22381 tcaaaccttc cggcgtcgat tacagcgtca tgaccgctga cgatatggtc gtggttagca
    22441 tcgaaaccgg tgaagtggtt gaaggtacga aaaagccctc ctccgacacg ccaactcacc
    22501 ggctgctcta tcaggcattc ccctccattg gcggcattgt gcatacgcac tcgcgccacg
    22561 ccaccatctg ggcgcaggcg ggtcagtcga ttccagcaac cggcaccacc cacgccgact
    22621 atttctacgg caccattccc tgcacccgca aaatgaccga cgcagaaatc aacggcgaat
    22681 atgagtggga aaccggtaac gtcatcgtag aaacctttga aaaacagggt atcgatgcag
    22741 cgcaaatgcc cggcgttctg gtccattccc acggcccgtt tgcatggggc aaaaatgccg
    22801 aagatgcggt gcataacgcc atcgtgctgg aagaggtcgc ttatatgggg atattctgcc
    22861 gtcagttagc gccgcagtta ccggatatgc agcaaacgct gctggataaa cactatctgc
    22921 gtaagcatgg cgcgaaggca tattacgggc agtaatgact gtataaaacc acagccaatc
    22981 aaacgaaacc aggctatact caagcctggt tttttgatgg attttcagcg tggcgcaggc
    23041 aggttttatc ttaacccgac actggcggga caccccgcaa gggacagaag tctccttctg
    23101 gctggcgacg gacaacgggc cgttgcaggt tacgcttgca ccgcaagagt ccgtggcgtt
    23161 tattcacgcc gatcaggttc cccgcgctca gcatattttg cagggtgaac aaggctttcg
    23221 tctgacaccg ctggcgttaa aggattttca ccgccagccg gtgtatggcc tttactgtcg
    23281 cgcccatcgc caattgatga attacgaaaa gcgtctgcgt gaaggtggcg ttaccgtcta
    23341 cgaggccgat gtgcgcccgc cagaacgcta tctgatggag cggtttatca cctcaccggt
    23401 gtgggtcgag ggtgatatgc acaatggcac tatcgttaat gcccgtctga aaccgcatcc
    23461 cgactatcgt ccgccgctca agtgggtttc tatagatatt gaaaccaccc gccacggtga
    23521 gctgtactgc atcggcctgg aaggctgcgg gcagcgcatc gtttatatgc tggggccgga
    23581 gaatggcgac gcctcctcgc ttgatttcga actggaatac gtcgccagcc gcccgctgct
    23641 gctggaaaaa ctcaacgcct ggtttgcaaa ccacgatccc gatgtgatca tcggctggaa
    23701 cgtggtgcag ttcgatctgc gaatgctgca aaaacatgcc gagcgttacc gtcttccgct
    23761 gcgtcttggg cgcgataata gcgagctgga gtggcgcgag cacggcttta aaaacggcgt
    23821 cttttttgcc caggctaaag gtcggctaat tatcgacggt atcgaggcgc tgaaatccgc
    23881 gttctggaat ttctcttcat tctcgctgga aactgtcgct caggagctat taggcgaagg
    23941 aaaatctatc gataacccgt gggatcgaat ggacgaaatt gaccgccgtt tcgccgaaga
    24001 taaacctgcg ctggcaactt ataacctgaa agattgcgag ctggtgacgc agatcttcca
    24061 caaaactgaa atcatgccat ttttactcga acgagcaacg gtgaacggcc tgccggtgga
    24121 tcggcacggc ggttcggtgg cagcgtttgg tcatctctat tttccgcgaa tgcatcgcgc
    24181 tggttatgtc gcgcctaatc tcggcgaagt gccgccgcac gccagccctg gcggctacgt
    24241 gatggattca cggccagggc tttatgattc agtgctggtg ctggactata aaagcctgta
    24301 cccgtcgatc atccgcacct ttctgattga tcccgtcggg ctggttgaag gcatggcgca
    24361 acctgatcca gagcacagta ccgaaggttt tctcgatgcc tggttctcac gggagaaaca
    24421 ttgcctgccg gagattgtga ctaacatctg gcacgggcgc gatgaagcca aacgtcaggg
    24481 taataagccg ctttcgcagg cgctgaagat cattatgaat gccttttatg gcgtgctcgg
    24541 caccaccgcc tgccgcttct tcgatccgcg cctggcatcg tcgatcacca tgcgtggtca
    24601 tcagatcatg cggcagacca aagcattgat tgaagcgcag ggctacgaca ttatctacgg
    24661 agataccgac tcgacttttg tctggctaaa aggtgcacat tcggaagaag aggcggcgaa
    24721 aatcggtcgt gcactggtgc agcacgttaa cgcctggtgg gcggaaacgc tgcaaaaaca
    24781 acggctgacc agcgcattag aactggagta tgaaactcat ttctgccgtt ttcttatgcc
    24841 aaccattcgc ggagctgata ccggcagtaa aaagcgctac gccgggctga ttcaggaggg
    24901 cgacaagcag cggatggtgt ttaaagggct ggaaaccgtg cgcaccgact ggacgccgct
    24961 ggcccagcag tttcagcagg agctgtacct gcgtatcttc cgcaacgagc catatcagga
    25021 gtacgtccgc caaaccatcg acaaactgat ggcgggtgaa ctggatgcgc ggctggttta
    25081 ccgtaaacgc cttcgccgtc cgctgagcga atatcagcgt aatgttccgc ctcatgtacg
    25141 cgccgctcgc cttgccgatg aagaaaatca aaagcgcggt cgccccttgc aatatcagaa
    25201 tcgcggcacc attaagtacg tatggaccac caacggcccg gagccgctgg actaccaacg
    25261 ttcaccactg gattacgaac actatctgac ccgccagcta caacccgtgg cggagggaat
    25321 actccctttt attgaggata attttgctac acttatgacc gggcaacttg ggctattttg
    25381 agcaaaaaaa agagttcgcc agataccatt ttgatgcgtg acgaatgctt tgccatccag
    25441 taccatagcg ccctttccat tcctggacct gaataacacc actacctcat aagcacggta
    25501 gcgggtggtt attgcctgca attaaagata tagagccgaa cacatatgcc ttttacactt
    25561 ggtcaacgct ggatcagcga tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg
    25621 gatgcgcgaa ctgtcacttt acttttccca tctactggtg aaaaccgtct gtacgcacgc
    25681 agtgattccc ccgtgacccg cgtgatgttc aaccctggtg ataccattac cagccatgac
    25741 ggctggcaga tgcaagtcga agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt
    25801 actcgcctgg atactgaaga gtccggcgta gccctgcgtg aagttttcct tgatagcaaa
    25861 ctggtgttca gcaaaccgca ggaccgtctg tttgccgggc agattgaccg tatggaccgc
    25921 tttgcgctgc gttatcgcgc gcgtaagtat tccagcgaac agttccgtat gccgtacagc
    25981 ggcctgcgcg gtcagcgtac cagcctgatc ccgcatcagc tcaacatcgc tcatgatgtt
    26041 ggtcgccgcc acgcgccgcg cgtcctgctg gctgacgaag tgggtttagg gaaaaccatt
    26101 gaagccggga tgatcctgca tcagcaactg ctctctggcg ctgctgaacg tgtgctgatt
    26161 atcgtcccgg aaaccttaca gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg
    26221 cgctttgctc tgtttgatga tgagcgttat gccgaagctc agcacgatgc ttacaacccg
    26281 tttgacaccg aacagctggt gatttgctcg ctggattttg cccgtcgtag caaacagcgc
    26341 ttggaacatc tctgtgaagc cgaatgggac ctgctggtgg tcgatgaagc gcatcacctg
    26401 gtgtggagcg aagatgcgcc aagccgtgaa tatcaggcca ttgaacaact ggcagagcac
    26461 gtgccgggcg ttctgctgct gaccgcgacc ccggaacagc tggggatgga aagccacttc
    26521 gcccgtctgc gtctgctgga cccgaaccgt ttccacgatt ttgcgcagtt cgttgaagag
    26581 cagaaaaatt atcgtccggt tgcggacgcc gttgccatgc tgctggcagg taacaaactg
    26641 agcaatgacg aactgaacat gctcggcgag atgatcggcg agcaggatat cgagccgctg
    26701 ttgcaggcag caaacagcga cagcgaagat gcccagagcg cccgtcagga gctggtttcg
    26761 atgctgatgg atcgccacgg caccagccgc gtgctgttcc gtaacacgcg taacggtgtg
    26821 aaaggattcc cgaaacgcga gctgcacacc attaagctgc cgctaccgac gcagtatcag
    26881 acggctatta aagtctccgg cattatgggc gcacgtaaaa gtgcggaaga tcgtgctcgc
    26941 gatatgctct acccggagcg tatttatcag gaatttgaag gtgataacgc cacctggtgg
    27001 aacttcgatc cgcgcgttga gtggctgatg ggctacctga ccagccatcg ctctcagaaa
    27061 gtgctggtga tctgcgccaa agctgccact gcgctgcaac tggagcaggt actgcgcgaa
    27121 cgtgaaggta ttcgcgctgc ggtgttccac gaaggtatgt cgattatcga acgtgaccgc
    27181 gctgccgcct ggtttgccga agaagacacc ggcgcacagg tactgctgtg ctcagaaatc
    27241 ggttctgaag gacgtaactt tcagttcgcc agccacatgg tgatgtttga cctgccattc
    27301 aacccggatc tactggagca gcgtattggt cgtctggatc gtatcggcca ggcgcacgat
    27361 attcagatcc atgtgcctta tctggagaaa accgctcagt cggtgctggt gcgctggtat
    27421 cacgaaggtc tggatgcatt tgagcacacc tgcccgaccg gacgcactat ttacgatagc
    27481 gtatacaacg atctgattaa ctatctggct tcaccggatc aaaccgaagg ctttgacgat
    27541 ctgatcaaaa gctgccgcga gcaacatgaa gcgctgaaag cacagctgga acagggtcgt
    27601 gaccgcctgc tggaaatcca ctccaacggt ggcgaaaaag cccaggaact ggcagaaagc
    27661 attgaagagc aggatgacga taccaacctg atcgccttcg ccatgaacct gttcgatatt
    27721 atcggtatca atcaggacga tcgcggcgac aacatgatcg tgctgacgcc gtccgatcat
    27781 atgctggtgc cggacttccc tggcctgtcg gaagatggca tcaccatcac ctttgatcgt
    27841 gaagtggcgc tggcgcgtga agatgcacag tttattacct gggagcatcc gctgatccgc
    27901 aacggtctgg atctgatcct ttctggcgat accggtagca gcacgatttc actgttaaaa
    27961 aacaaagcgt tgccggtagg tacgctgttg gtggaactga tttatgtggt tgaagcccag
    28021 gctccgaagc agttgcagct caaccgcttc ctgccaccga cgccggtacg tatgctgctg
    28081 gataaaaacg gcaacaacct ggcggcgcag gtagagtttg aaacctttaa ccgccagctt
    28141 aacgcggtta accgtcacac cggcagcaaa ctggttaacg ccgtgcagca ggatgttcac
    28201 gctatccttc aactgggtga agcgcagatc gagaaatctg cccgtgcatt gattgatgca
    28261 gcgcgtaacg aagccgacga aaaactgtct gccgagctgt ctcgtctgga agctctgcgt
    28321 gcagtgaacc cgaacattcg tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag
    28381 gtaatggaaa gcctggatca ggcaggttgg cgtctggatg ccctgcgttt gatcgttgta
    28441 acgcatcagt aacggagccg aaaatgggga tggaaaacta caatccaccg caggaaccct
    28501 ggttggttat cctgtatcag gatgaccata ttatggtggt caacaagccg agcggtttgt
    28561 tgtcagtgcc gggtcgtctg gaagagcaca aagacagcgt gatgacgcgc attcagcgtg
    28621 attatccgca ggcagaatcg gtgcatcgtc tggatatggc taccagcggc gtgattgtag
    28681 tggcgctgac caaagccgcg gagcgggagt taaaacgcca gttccgcgag cgcgagccga
    28741 aaaagcagta tgtggcccgc gtctgggggc atccatcccc cgcagaaggt ctggtggatc
    28801 tgccgctgat ttgcgactgg ccaaaccgcc cgaaacagaa agtctgttac gaaacgggta
    28861 aacctgcgca gacggaatat gaagtggtgg agtatgcggc ggataacacg gcaagagtgg
    28921 tgttaaaacc gattaccggg cgttcgcatc aattgcgtgt gcatatgctg gcgctggggc
    28981 atccgattct cggcgatcgt ttttatgcat caccagaagc gagagcgatg gcaccgcgtt
    29041 tgttgctaca tgcagagatg ttgacgatta cccatccggc gtatggcaat agtatgacgt
    29101 ttaaagcgcc agcggatttt taatcgtcac gctggcgcag ttcggtaggc ggataaggcg
    29161 ttcacgccgc atccggcaat tgtgcgccga ttgcctgatg cgacgcgggc cgcgtcttat
    29221 caggcctaca gtttaaaact tatttaaacc ctttctgctg ctttatcagc tcatatgcct
    29281 gctgaatttc ctgcgctttc tgcttcgcca tctccatcat ctcaggcggc aaacctttcg
    29341 ccaccagctt atcgggatgg tgttcactca tcagcttacg gtaggcacgt ttgatggtgg
    29401 tcgcatcatc cgtcggcttc acgcccagca cattacaggc atcttccagc gtcgggccac
    29461 gctgcgcttg ctgccagtta ccaccgccag attgctgctg ataaccgccg ccaaactgtg
    29521 caccgccctg catcatgcgc aaaaactggt caaactgggc gcgggagatc cctaattctt
    29581 cagcaatgac atacagcacc gcccgttcat tcgggtgcag tgaaccatca gcaaacgccg
    29641 cctgaatctg gatctccaga aacatacgaa ttaagtcaaa acgaccaaag cagacactgc
    29701 gaaactggcg catcttttcg cgcagcgggt aattatctga ttttcccacc cggaacgcgt
    29761 tttgcgccgc agtacgggaa gcgccatgaa ggttcattcg gtccatcaac tggctggcga
    29821 tatgaatatc agcctccgtg acgcgacctt tggatttggt taaatgcccc atcacttcaa
    29881 aagtggtggc aaaaaacagc gcctgacgct cacgctggtt ggcgaaccac gccattttac
    29941 ggctacgggc tttatcaaac atatggccaa ttaacaggcc taacactacg ccccaaaagc
    30001 cgccgcccat cagtaaggcc acggccacgc caatgatttt tccccaatac tgcatatatt
    30061 ccccaaatcg acacacggat atcagggcta tctcccacaa tataaaggtg cttttaccgt
    30121 tttccggctg cggtcaattg tgggacatcg cctataattt gcattatcat acctgtcatt
    30181 caatgccgtg cctaacacca ctgacgctat tcggacagga ttaacactag cgtagagatg
    30241 acgagtacgt tagtctctga ccgtttgtca cgcgcaacgt taccgatgat ggaacaataa
    30301 aatcaacgta tgaaaaaacg tatccccact ctcctggcca ccatgattgc caccgccctt
    30361 tatagtcaac agggactggc agccgacctc gcctcacagt gcatgttggg cgtgccaagc
    30421 tatgaccgtc ctctggtaca gggcgatacc aatgacttac ccgtgactat caatgctgac
    30481 cacgcgaaag gggactaccc ggatgacgcc gtgtttactg gcagcgtgga tatcatgcag
    30541 ggtaacagcc gtctgcaggc cgacgaagtg cagctccatc aaaaagaggc accaggacaa
    30601 ccggagccgg tacgtaccgt tgatgcgctc ggtaatgtcc attacgacga taaccaggtg
    30661 atcctcaaag ggccgaaagg ctgggcgaat ctgaacacca aagataccaa cgtctgggaa
    30721 ggtgattacc agatggtggg tcgccagggt cgcggtaaag cggacctgat gaaacaacgt
    30781 ggcgaaaacc gctataccat tctggataac ggtagcttta cctcctgtct gccgggttct
    30841 gacacctgga gcgtggtagg tagcgaaatt attcatgacc gcgaagaaca agttgcggag
    30901 atctggaacg cccgctttaa ggtgggtccg gtaccgatct tttatagccc ctatttgcag
    30961 ttgccggtgg gtgacaaacg tcgctctggt ttcttgatcc cgaacgccaa gtacaccacc
    31021 accaactact ttgagttcta cctgccatat tactggaaca tcgcgccaaa tatggatgcc
    31081 accatcacgc cgcattatat gcatcgtcgt ggcaacatca tgtgggagaa cgaattccgc
    31141 tacctctccc aggcgggcgc tggcttgatg gaactggact atctgccttc agataaagtc
    31201 tatgaagatg aacacccgaa cgatgacagt tcacgtcgtt ggttattcta ctggaaccac
    31261 tccggggtca tggatcaggt gtggcgtttc aacgtcgact acaccaaggt cagcgatcct
    31321 agctacttca atgatttcga taacaagtac ggttccagta ctgacggcta cgcaacgcaa
    31381 aaattcagcg ttggctatgc ggtgcaaaac ttcaatgcca ccgtttcaac caagcagttc
    31441 caggttttca gcgaacagaa caccagtagc tactcggcag agccgcagtt agacgttaat
    31501 tactaccaga atgatgttgg tccgtttgat acgcgtattt acggccaggc agtgcacttt
    31561 gttaacacca gagacgacat gcctgaagca acccgtgttc acctggaacc gaccatcaat
    31621 ttgccgctct ctaataactg gggcagcatc aataccgaag cgaagtttct ggcaacccat
    31681 tatcagcaaa ccaatcttga ctggtataac tccagaaaca cgaccaagct ggacgaatcc
    31741 gttaaccgcg taatgccgca attcaaagtt gacggcaaaa tggtctttga acgcgatatg
    31801 gaaatgctgg ctccgggtta tacccaaacg ctggaaccgc gcgcgcagta tttgtacgtg
    31861 ccgtatcgcg atcagagcga catctataac tacgactcgt ctctgctgca atctgactac
    31921 tctggcctgt tccgggaccg gacttacggc ggtcttgacc gtattgcctc cgctaaccag
    31981 gtgacgaccg gtgtcacatc tcgcatatat gatgatgctg ccgttgaacg ttttaatatt
    32041 tccgttggtc aaatctacta tttcacggag tctcgcactg gcgatgacaa cataacatgg
    32101 gagaatgacg acaaaacggg ctcactggtg tgggcaggcg acacctactg gcgtatctcc
    32161 gagcgttggg gattgcgtgg cgggattcag tacgatacac gtctggataa cgtagcgacc
    32221 agtaactcca gcattgaata ccgtcgggat gaagaccgtc tggtacagct gaattaccat
    32281 tacgccagcc cggaatatat tcaggctacg ctgcctaagt actattccac tgctgagcaa
    32341 tataagaatg gtatttcgca ggtaggtgct gtcgccagca ggccaattgc cgatcgttgg
    32401 tccattgttg gggcctacta ctacgacacc aatgcgaaca agcaagccga ctctatgtta
    32461 ggtgtgcaat acagctcctg ctgctatgca attcgcgtcg gttacgagcg gaagctgaac
    32521 ggttgggata acgataaaca acatgcggta tatgacaacg caatcggctt taacatcgaa
    32581 cttcgcggcc tgagctccaa ctacggtctg ggtacgcaag agatgctgcg ttcgaacatt
    32641 ctgccgtatc aaaacacttt gtgatttgtt gatttaccac gtaatccgca gtgcggttaa
    32701 ttgaaatgga aaaagtatga agaactggaa aacgctgctt ctcggtatcg ccatgatcgc
    32761 gaataccagt ttcgctgccc cccaggtagt cgataaagtc gcagccgtcg tcaataacgg
    32821 cgtcgtgctg gaaagcgacg ttgatggatt aatgcagtcg gtaaaactga acgctgcgca
    32881 agcaaggcag cagcttcctg atgacgcgac gctgcgccac caaatcatgg aacgtttgat
    32941 catggatcaa atcatcctgc agatggggca gaaaatggga gtgaaaatct ccgatgagca
    33001 gctggatcag gcgattgcta acatcgcgaa acagaacaac atgacgctgg atcagatgcg
    33061 cagccgtctg gcttacgatg gtctgaacta caacacttat cgtaaccaga tccgcaaaga
    33121 gatgattatc tctgaagtgc gtaacaacga ggtgcgtcgt cgcatcacca tcctgccaca
    33181 ggaagtcgaa tccctggcgc agcaggtggg taaccaaaac gacgccagca ctgagctgaa
    33241 cctgagccac atcctgatcc cgctgccgga aaacccgacc tctgatcagg tgaacgaagc
    33301 ggaaagccag gcgcgcgcca ttgtcgatca ggcgcgtaac ggcgctgatt tcggtaagct
    33361 ggcgattgct cattctgccg accagcaggc gctgaacggc ggccagatgg ggtggggccg
    33421 tattcaggag ttgccgggga tcttcgccca ggcattaagc accgcgaaga aaggcgacat
    33481 tgttggcccg attcgttccg gcgttggctt ccatattctg aaagttaacg acctgcgcgg
    33541 cgaaagcaaa aatatctcgg tgaccgaagt tcatgctcgc catattctgc tgaaaccgtc
    33601 gccgatcatg actgacgaac aggcccgcgt gaaactggag caaattgctg ctgatatcaa
    33661 gagtggtaaa acgacttttg ctgccgcagc gaaagagttc tcgcaggatc caggttctgc
    33721 taaccagggc ggtgatctcg gctgggctac accagatatt ttcgatccag ccttccgtga
    33781 cgccctgacc cgcctgaaca aaggtcaaat gagtgcaccg gttcactctt cattcggctg
    33841 gcatttaatc gaactgctgg atacccgtaa tgtcgataaa accgacgctg cgcagaaaga
    33901 tcgtgcatac cgcatgctga tgaaccgtaa gttctcggaa gaagcagcaa gctggatgca
    33961 ggaacaacgt gccagcgcct acgttaaaat cctgagcaac taatggttaa aacccaacgt
    34021 gttgtgatca ctcccggcga gcccgccggg attggcccgg acttagttgt ccagcttgca
    34081 cagcgtgagt ggccggtcga actggttgtt tgtgccgatg ccactctcct taccgaccgg
    34141 gcagcgatgc tcggtttgcc gctcaccctc cgcccttatt cccccaactc ccctgcacaa
    34201 ccgcaaacta cgggcacatt aacgctactt cctgtcgcgc tacgtgaatc tgtcactgcg
    34261 gggcagttag cgattgaaaa tgggcattac gtggtggaga cgctggcgcg agcgtgtgat
    34321 ggctgtctga acggtgaatt tgctgcgctg atcacaggcc ccgtgcataa aggcgtcatt
    34381 aacgacgcaa gcattccgtt taccggtcat accgagtttt tcgaagagcg ttcgcaggcg
    34441 aaaaaagtgg tgatgatgct ggcgaccgaa gaacttcgcg tggcgctggc aacgacgcat
    34501 ttaccgctgc gcgatatcgc agatgctatc acccctgcac ttttgcacga agtgattgct
    34561 attttgcatc acgatttgcg tacaaaattt ggtattgccg aaccgcgcat tctggtctgc
    34621 gggctgaatc cgcacgcggg cgaaggcggt catatgggta cggaagagat agacaccatt
    34681 attccggtgc tcgacgagct gcgggcgcag gggatgaaac tcaacgggcc gctgcctgcc
    34741 gataccctgt ttcagccgaa atatctcgat aacgccgacg ccgtgctggc gatgtaccac
    34801 gatcagggtc ttcccgtgct aaaataccag ggcttcgggc gcggtgtgaa cattacgctg
    34861 ggcctgccct ttattcgcac atcagtggac cacggcaccg cgcttgaact ggcgggacgt
    34921 ggcgaagccg atgtcggcag ttttattacg gcgcttaatc tcgccatcaa aatgattgtt
    34981 aacacccaat gaataatcga gtccaccagg gccacttagc ccgtaaacgc ttcgggcaaa
    35041 actttctcaa cgatcagttc gtgatcgaca gcattgtttc tgccattaac ccgcaaaagg
    35101 gccaggcaat ggtcgaaatc ggccccggtc tggcggcatt gaccgagccg gtcggcgaac
    35161 gtctggacca gctgacggtc atcgaacttg accgcgatct ggcggcacgt ctgcaaacgc
    35221 atccattctt aggcccgaaa ctgacgattt atcagcagga tgcgatgacc tttaactttg
    35281 gcgaactggc cgagaaaatg ggtcagccgc tgcgtgtttt cggcaacctg ccttataaca
    35341 tctccacgcc gttgatgttc catctgttta gctatactga tgccattgcc gacatgcact
    35401 ttatgttgca aaaagaggtg gtgaatcgtc tggttgcagg accgaacagc aaagcgtatg
    35461 gtcgattaag cgtcatggcg caatactatt gcaacgtgat cccggtactg gaagttccgc
    35521 cgtcagcctt tacaccacca cccaaagtgg attccgccgt cgtgcgcctg gtccctcatg
    35581 caacgatgcc tcacccggtt aaagatgttc gcgtgttgag ccgcatcacc accgaagcct
    35641 ttaaccagcg tcgtaaaacc attcgtaaca gcctcggcaa cctgtttagc gtcgaggtgt
    35701 taaccggaat ggggatcgac ccggcgatgc gagcggaaaa tatctctgtc gcgcaatatt
    35761 gccagatggc gaactatctg gcggagaacg cgcctttgca ggagagttaa cgatgatcaa
    35821 ttcgccccga gtgtgtattc aggttcaaag cgtctacatt gaggcacaat cttcacctga
    35881 taatgaacgt tacgtttttg cttataccgt aaccatacgc aatctggggc gagcgccagt
    35941 gcagttgttg gggcgttact ggctgatcac caatggcaat ggccgtgaaa ccgaagtcca
    36001 gggcgaagga gtggttggcg tccagccact tatcgcgcct ggcgaagagt accagtacac
    36061 cagcggtgca atcattgaaa ccccgctggg caccatgcag ggtcactacg aaatgatcga
    36121 tgaaaatggc gtccctttca gcatcgacat tcccgtattc cgactcgccg ttcccacact
    36181 cattcattaa aagaatatgg cgacatacct tattggcgac gttcatggtt gttacgatga
    36241 actgatcgca ttgctgcata aagtagaatt tacccccggg aaagataccc tctggctgac
    36301 gggcgatctg gtcgcgcgcg gcccgggttc gctggatgtt ctgcgctatg tgaaatcctt
    36361 aggcgacagc gtacgtctgg tgctgggcaa tcacgatctg catctgctgg cggtatttgc
    36421 cgggatcagc cgcaataaac cgaaagatcg cctgacaccg ctgctggaag cgccggatgc
    36481 cgacgagctg cttaactggc tgcgtcgcca gcctctgctg caaatcgacg aagagaaaaa
    36541 gctggtgatg gcccacgcag ggatcacgcc gcagtgggat ctgcagaccg ccaaagagtg
    36601 cgcgcgcgat gtagaagcgg tgctgtcgag tgactcctat cccttctttc ttgacgctat
    36661 gtacggcgat atgccaaata actggtcacc ggaattgcgg gggctgggaa gactgcgttt
    36721 tatcaccaac gcctttaccc gtatgcgttt ttgcttcccg aacggtcaac tggatatgta
    36781 cagcaaagaa tcgccggaag aagcccctgc cccactgaaa ccgtggtttg cgattcctgg
    36841 ccctgtcgct gaagaataca gcatcgcctt tggtcactgg gcatcgctgg aaggcaaagg
    36901 tacgccggaa ggtatttacg cgctggatac cggctgctgc tggggtgggt cattaacctg
    36961 cctgcgctgg gaagataaac agtattttgt ccagccgtcg aaccggcata aggatttggg
    37021 cgaagcggcg gcgtcttaaa cacagcctga tacaggaagg ccggataaga cgcgaccggc
    37081 gtcgcatccg gcactagccg taaattctat acaaacttac cgccgctcca gaatctcaaa
    37141 gcaatagctg tgagagttct gcgcatcggc gtcgtggaat tcgctgaata ccgattccca
    37201 gtcatccggc tcgtaatccg ggaaatgggt gtcgccttcc acttctgcgt cgatatgcgt
    37261 cagatacagt ttttgcgctt ttggcaggaa ctgctcataa acgcgaccgc cgccaatcac
    37321 catgatttct ggtacgtcac cacacgccgc aatggcttca tccaccgatt tcacccacgt
    37381 tacgcgatcg tccgtacccg gttgactgct gaggataata tttttgcgtc ctggcaacgg
    37441 acgaccgatt gattcccaag tatggcggcc cataatcacg ggtttattta aggtgttgcg
    37501 tttaaaccag gcgagatcgg caggcaggtt ccacggcatg gcgttttcca tgccgataac
    37561 gcgatctacc gctaacgccg caatcagact gatcattgag atttcccgat aaaaaaattg
    37621 tcgccactat acgtaaagcg taaaccgtcg tcgactggtg cgaggatgat gttgaggaaa
    37681 attttatatt ctgctggcga gtccacgctc tccccctgga ctcgccgcat tacaatgaaa
    37741 caaaaacaaa cagttagctg taaagtgtga tttacgtcac tctttattaa gatgagggtt
    37801 tcgtttccgg ttcatccgcc atgttgccgg tatgtttacc ttcttctgtt ccctgccagc
    37861 catgtcgttg aattaatgac agatgttcgc ggtcctcggt gatgatctca cttaacatcg
    37921 cgctggtgcg tttatagacc gccgcgcggg ctttggtgtc attctcaacc attgccatct
    37981 cttccaccat ctgaatatta aagcggcgga acacatcggc acgttctcgc gcttcatacg
    38041 gccccagacc taaactttcc agcgccagac gcccggtttt cagcgcacct tcaaaggttt
    38101 cacgctccgg cttttcaacg cctgcctgac gcaaacgaat gtagtgatcg acatcgcggg
    38161 cgcgggcaat aatctgcaaa tgcgggaaat gttctttcac catctccgtc agttgcaggt
    38221 tggtttgcgg atcgtcaata gcgttaatca gcacttccgc tttcgccgct ccggcagatt
    38281 ccagtaaatc catccgcgtg gcatcgccat aaaacacttt cataccaaat ttacgcagcg
    38341 tttcgatatg gtccggatcg tgatcgagca ccaccatttt caccccgctg gagagcagta
    38401 aacgtccggt aatctgtcca aaacgaccga atccggcgat aatcacgcgc ggctgttctt
    38461 catcgatctc atcggcttca cgcgcctcct ccgtagaaga ttgctcaagg cgattgagga
    38521 tcaccagcag aatcggcgtt gctgccatcg acagcgccac cgccagggtc agcgatttcg
    38581 cccactccgg ctccagcaca ttcgccatct gcgccgcacc aaataccaca aaggcaaact
    38641 cactgccctg ccctaacaac accgcaaacc agcgacgctg tttatttggc acctgcaacg
    38701 gtcgggcaat cagccacagc atggcgattt tgatgatgag gaaaccgagc agcaaaatga
    38761 caatgcgcaa tgggttttca atcagcgtgc caaagtctat cgacatgcca acaccgatga
    38821 aaaacagccc caacagcaaa cctttaaacg gttcgatatc gctttccaac gcatgacggt
    38881 attccgagct tgccagcagt acgcccgcca gaaacgcgcc catcgccatc gacaagccga
    38941 cctcttccag cagcaaacca aagccaaaca caaggaataa cgccacggca ctaaacactt
    39001 cccgcaagcc agagcgggca acaaaacgca gcgccggacg cgtaacatag cgccccagca
    39061 ataccaccag taccagcgca cccgccactt ttaacgccga gagagcaaat gcgcccatcg
    39121 tcgtcgaggc actgctcgcc gccagtagcg ggatcatcgc caccagcggg attgccgcga
    39181 tatcctggaa cagcagcact gcaaaggcac tgcggcccat ttgcgtcacc atcagattgc
    39241 gttcattcat cgcctgcatg gcaatcgccg ttgatgagag cgccagcgtc ataccgatca
    39301 attccgcgac ctgccagcgc agcccaagta acatacagaa cagccccagc aggccaccgc
    39361 aaatcaccat ctgcaatgcg ccaccgccga acactgccgc acgcagcttc cacagccttt
    39421 gtggatcgag ttcgaggccg ataataaaca gcatcagcac caccccaatc tcggcaaagt
    39481 gcagaataga ttcggcatcg gtcaccagtc gcagccccca cgggccaata atgcagccgg
    39541 cgatcaggta gccaagtacc gatcccagac caagacgtac cgcaatgggt acaatcagcg
    39601 ccgccgatcc gaggtagatc agcgcctgaa ccagcgtatg actatccatg atgggcctcc
    39661 tgccattcca gcagacgttg cttatagtgg cgcgcctgcc cttcgagggt ttcgtcgtca
    39721 caaataaagg tgcagtgcat ggcaaacggt ggcagccagt tcagcccgca gtagattgcc
    39781 gtcgcctgta gcggctgcga cagcacatca aagcccggat gcgcaccaat ttcaaaatgg
    39841 ctttccccgc cgccggtcgt caccgcccac agcaaatgtt tgccatgcag cgccgtgccg
    39901 ccgtgaccgt aagcccagcc gtgcgacaaa actttatcga tccaaagttt aagaagcgga
    39961 ggaatgctgt accactgcat cgggtgctgc cagacgatca gatcggcgcg agagagcgcc
    40021 tcctgctcgg cggcaatatc gatattgaag tcaggataga gttgataaag agagcgaatt
    40081 tcgacgcctt ccagcgtcct tgcctgttca agcatccgtt tattcgcatg ggaatgatgc
    40141 ggatacggat gcgcataaat gatgaggatc atagttatta gcccgcgtgt ttacgccttt
    40201 gttttcccac aaagtgtagt catatattca cgcggcttat agtcaataat attgattcag
    40261 aaaagagaga aatctgaaca aataatccca ttctctgccc gcggatcgtc atattgctgg
    40321 tctgcgccag tgacattcct cgagtctccg gggcaaacgc tacggaaatc aacaagccaa
    40381 acagcgagat acccgccccc attagcatcg tgttactgat accgtaatta ttgataaata
    40441 tcggcagtgc ccaggtcgaa acaatggtgc caatacgact taaggacata atcacgccca
    40501 cggcagaggc gcggatatcc gtcgggaaga gttcattagg atagagccac tgcaaattac
    40561 ccggcccgcc agagaaaaag gcatacaccg caaaggccat cactaccagc cagatcccca
    40621 tatccgggat tagccccaaa accgccagcg ccagcgtcat catggcaaag ctgccaatca
    40681 acaatggacg ccgtccggca gtgtttagcc acagcatcgg cggaatacag ccgagcataa
    40741 agaacaggct aatcaccaca ttccctagtg ccgcgttttt gccaaccccc aatcccaacg
    40801 aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat tgggatcacc tggcaggtcc
    40861 agatggcggc aacaaacaga acaaaaggaa aatggcggcg attaaacaga tcgcgaaaac
    40921 gggtttgctg cggctgctct tcatcgaaag ccaccggttc gccaaacagt tttatcatca
    40981 tctcttcgca ctcttttact cgccctttgc gtaataacca gcggggagat tctggcagtt
    41041 cgaatcgacc aatcaaaatc aacaaacaag ggatcgccgc gctacccagc atccagcgcc
    41101 aaccaccttc cacatcataa agccagtagc cgaccagatc ggcgcaggtc gcgccgacat
    41161 accacatcgc ggcaataaag ctgatggaaa acgcccgctg acgggtactg gagaactcgg
    41221 tgatcatcga agtggcgatg ggataatctg caccgatgac aatgccgatg agtacccgca
    41281 tcaccaacag ttcgacgggg gatgaaacac acatcgtcgc caccgatatc acgccgatgg
    41341 cgatgatatc aatgaggaac attttgcgcc gtccgacttt atcggagata tagccaaaca
    41401 gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc cagcaagcca atccagtcag
    41461 cgtccagttt cagcgccgga gtcagttgct ccagcgccac gccaatcatt accagtacat
    41521 aaccatccag aaacggacca ccgcttcccc acagcaaaat gcggcggtga atagaggaga
    41581 atttgagatc gtcaaagttt ctggacggtt gcatgggtta ttcctgtttt tttgcaatga
    41641 gagtcagtac ggcgttacgt atcaaaccaa catcagccgt aacggaactc cacaccaaag
    41701 gtgccgcgcg ggtattccca ctgttccagc gccgacccca gccccagaat gcgacaggtg
    41761 ccgcactcca gacatccggc gtaatcgaag cgcacactgc cgtcatcctg cttcttgtac
    41821 agacctgcgg ggcacgcttt caccagcagc tccagcacct gtttatcagc atcggccttc
    41881 acaacaatgt gcggatgctc ttcatcgaca ttgaatttat tgacgcccag tttgacgtcc
    41941 acattgacgg gagaagtcat aaaacggtca ctcctttcat gccatccttg atcagattga
    42001 tgaagcccac tttcttgccg tggcggagga ttttcttgcg catcagttcc ggcgcactgc
    42061 cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag ttccgggttg ccgctaaaca
    42121 tgcgtgggtt atcaaggaac gccggtagtt tctggtacat gcgcatatcg cgcagcgggc
    42181 cactctccag atgctgacga tattccgcga gtttttgctt actgaaatcg tcgcttttca
    42241 tcgctgaaag cacggttttt gctgcagctt ccccggcggc aatcgccaga tccataccgc
    42301 gaatggtaaa accgaggttc atacacattc cggcggcatc accggcaatc aatacgccgt
    42361 caccgaccag ctccggcagc atgttgatgc ctgcttccgg cactacgtga gcggaatatt
    42421 ccaccagctt gccgcccgcg atcagcggtg caacggccgg atgctgtttg aaatcttcca
    42481 gcatttgcgg caccgatttt ttcgcgtcat gcagatgatg cagaccacaa accagcccca
    42541 gcgacagggt attttcattg gtataaagga agccgccgcc catcaggcca tcggtgggtg
    42601 atcccgcaaa cagacaagcc gcgccctgat taccctgcaa ctgaaaacgg tcttcgatta
    42661 ccgacttcgg taactcgatc agttccttca cgccaaccgc cacatccgtc ggtttaacgc
    42721 gttttgccat ccccaacttt tcggcaagga tggagttcac cccatcagca aggagcaccg
    42781 ttttcgcttc aatcacatcg ccatcggctt ctacaccgac gactttgcca tcgcgctgta
    42841 cgaggttatc gacgcggatc ccggtaatta actgcgcgcc cgcttcttcg gcctgctcca
    42901 tcaaccaggc atcaaattta ctgcgcaaaa cggagtaaga acgctgggat ggcgaggttt
    42961 cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc cgtcataaac gcgagttttt
    43021 catgggtgat caggcgttct acgggggcgg aatcggcgaa acctggaata atgtgttcca
    43081 gactgtgggc atagagacgc ccgccagtaa cgttcttggc acctgcggaa ttgccacgct
    43141 cgataactaa cacttgcgcc ccttcgcggg cgagcaccag tgcggcaacc gaaccggcaa
    43201 gccctgcacc gacgatgatg gcgtcaaaga tatcttcgga cataagagct ccctgtcagc
    43261 ggcagagccg taatgcagcc ctgccagagt ggatcaacgc gctaaagctg cggtcagtgc
    43321 cggaaggatc ttcacggcat cgccaacaat gccgtaatcc gcgtactgga agatcggcgc
    43381 atttttatct ttattgatgg cgaaaatggt ttgcgacgcg ttagcgccaa ccatgtgctg
    43441 gatctacccg gagatcccca ccgccaggta cagttcaggt ttcagcatca ggttggagat
    43501 accgacatag cgttcgtgct ccatccattt ttcgttttct gccaccggac gagaacaggc
    43561 taactccgca cctatcgcct tgcaaagctg ttctgccagc gcaatgttct ctttgctgcc
    43621 aatgccgcga ccgacgctga ccaccagacg ggctttgtcg agatcgacgc tgttgctctg
    43681 gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga gcctgccact ccacggtgtg
    43741 cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg aacgtgccgc tgctgatggt
    43801 cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc gccagaccgc cgtaaaccat
    43861 gtgtttcact gttgctttac cgtcctgtac gctgacggtg ctggcatcgt tagacaccgc
    43921 cgctttaagg cgataaccca gttttgccgc cagtaatttg ccgcgacggg tgtttggcaa
    43981 cagcaccaga ccgtcagcgc cgtgctggcg aatagtgtca gccatgacac cggcgtaatc
    44041 ttcgatcatc cgatcgtccg gtttgccgtt taatttccag acatgattag cgccgagctg
    44101 gattgcctgt gcgccgtcgg catcattgag gacaaaggca ttgatttgat tagctaaagc
    44161 ctgcgcaccg ttcatcagtt ccggcagacg agagggggta tcgctgaata cccagacttg
    44221 agaaaacgtg ttcatagcat cccctgtaat taaatgactt tgcgaagatt ttcagcaaat
    44281 gcggcgatct gttcttcgcc gtcgccttca atcacgatgc gctgacgctc gcgctgtttc
    44341 ggcgcggcaa cctgttgttc agaccaggcg tccacggcgt taaaaccaat atccgccgcc
    44401 gaccatacct ggactggctt tttcgccgcg ccgagaatgg ctttcatcga aggaatttgt
    44461 ggggagttga tatcagtgga aacagcaaca accgcaggca gcggaatgct taaggtttcg
    44521 gtttcatctt ccagttcgcg ctcaacggtg aaggtatctg ccgtcaggga gataattttg
    44581 ctgacgccgt taactgccgg aatattgagg atttcgccca ccagcagacc aacctgctgg
    44641 gcataaaggt cggaagaacc atcgccacag aggatcagat caaagcctgc tttctgggcg
    44701 gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct gctcgaactg gtcatcaatc
    44761 accacaatca gttcatccgg gccgcgcgat agcacatctt tacgcccttt ggcgttggtc
    44821 agggctttac cgcccacact taaggctgtc acctgcgcct ctgctgcctg ttgctttagc
    44881 tggcaagccg cttcaatagc gttgagatcg tattggctta ttttggcatc ggctttgctg
    44941 aagtctaatg aaccatcagc attattgacc gcaatatcct gttcatcagg cacgcactta
    45001 tagcaagtaa taatcttcat tgcatctcca gaaatcatga aggaaacgtc gttcatatcg
    45061 gtggtgttaa ttacagatat cacgcccttt aatttggaaa taaaacttta atcaccaata
    45121 ttgaaaatgt cacacgcaga taaatccaac tttcaatatt gttaagttcc tcaccaatat
    45181 tgaaaacatg gcgtagcaaa aagaaatttt caatattgtt ttatagatca ccaatattga
    45241 aagtcagatg ctattttttt acccacaaaa gaatataaca aactgattaa taaccaaaat
    45301 taacgaaaaa cgccctctgt tcactcgaaa caaaaatgtg acaccaatca cagaatacag
    45361 cttattgaat acccattatg agttagccat taacgcgtcc acgaggttaa taataattat
    45421 attaaatgtt aacaaaaata aaacaaacgg gaacgcaaaa taaatattcg ttttcacagt
    45481 ggaattaact catgaagaat gaaaagagaa aaacgggaat agaaccgaag gttttctttc
    45541 cgccattaat aatcgtcggc atactttgtt ggcttacagt cagagatctg gatgcggcga
    45601 atgtcgttat taatgctgta ttcagttacg tcaccaatgt atggggatgg gcatttgaat
    45661 ggtatatggt ggtgatgctt ttcggttggt tctggctggt gtttggcccg tatgccaaaa
    45721 agcgtttagg taacgaaccg ccagaattta gcaccgccag ttggatcttt atgatgttcg
    45781 cctcctgtac gtctgctgcc gtactgttct ggggatcgat tgagatctac tactacatct
    45841 ccaccccgcc gtttggctta gaaccgaact cgacaggagc gaaagagttg gggctggctt
    45901 acagcttgtt ccactgggga cctctgccgt gggccactta cagcttcctt tcagtcgcct
    45961 tcgcttactt cttctttgtc cgcaaaatgg aagtgattcg ccccagctcg acactggtgc
    46021 cgctggtagg tgaaaaacac gccaaagggt tgttcggtac catcgtcgac aacttctatc
    46081 tcgtcgcctt gatcttcgcg atgggtacca gtctgggcct tgccacgccg ctggtgaccg
    46141 agtgtatgca atggttgttt ggcattccgc ataccctgca actggacgct atcatcatta
    46201 cctgctggat tatcctcaac gccatttgcg tcgcttgcgg tctgcaaaaa ggggtacgta
    46261 tcgccagtga cgtgcgtagt tacctgagct tcctgatgct gggttgggtg ttcatcgtca
    46321 gcggtgccag cttcatcatg aactacttca ccgattcggt ggggatgttg ctgatgtatc
    46381 tgccgcgcat gttgttctat accgatccca tcgctaaagg cggcttcccg cagggctgga
    46441 ccgtgttcta ctgggcatgg tgggtgattt acgccatcca gatgagtatc ttcctcgccc
    46501 gcatctcccg tggtcgtact gtgcgtgaac tgtgcttcgg catggtgatg gggctgacag
    46561 catccacctg gatcctgtgg accgtgctcg gtagtaacac tctgctgttg atggataaaa
    46621 acatcatcaa cattccaaat ctgatcgaac agtacggtgt ggcgcgcgcc atcatcgaaa
    46681 cctgggccgc tctgccactc agcaccgcca ccatgtgggg cttcttcatc ctctgcttta
    46741 ttgccaccgt tacgctggtt aacgcctgct cttataccct ggcgatgtct acttgccgcg
    46801 aagtacgcga tggtgaagaa ccaccgctgc tggtgcgtat cggttggtca attctggttg
    46861 gcattatcgg tattgttctg ctggcgctcg gcggcctgaa accgattcaa accgccatta
    46921 tcgccggagg atgcccgctg ttcttcgtca acattatggt gacgctctcc tttattaaag
    46981 acgcgaaaca gaactggaaa gattaattaa cccccaaaat atcaagaggt tgaaagatgg
    47041 attttaattt aaatgatgag caggaactgt ttgtcgccgg tatccgcgaa ctgatggcca
    47101 gcgaaaactg ggaggcctat tttgccgagt gcgaccgtga cagcgtctac ccggaacgtt
    47161 ttgtcaaagc actggcggat atgggtatcg acagtctgct gatccctgaa gagcacggtg
    47221 gtctggacgc ggggtttgtt actctcgccg ccgtgtggat ggagctggga cgtctggggg
    47281 caccaaccta tgtactgtac cagctgccgg gcgggttcaa taccttcctg cgcgaaggca
    47341 cacaagagca gatcgacaag attatggctt tccgcggcac cggtaagcag atgtggaact
    47401 cagcgattac cgaaccgggc gcgggctccg acgtgggtag cctgaaaacg acttataccc
    47461 gtagaaatgg taagatttat cttaatggta gtaagtgttt tattaccagt agcgcctaca
    47521 ccccgtacat cgtggtgatg gcgcgcgacg gggcttctcc ggacaaacct gtctacaccg
    47581 aatggtttgt tgatatgagc aaaccgggca tcaaagtgac caaacttgag aagctcggtc
    47641 tgcgtatgga tagctgctgt gaaatcacct ttgacgatgt ggaactggac gagaaagaca
    47701 tgttcggtcg ggaaggtaac ggctttaacc gcgtcaaaga agagttcgac catgaacgtt
    47761 tcctggtagc cctgaccaac tacggtacgg cgatgtgcgc ctttgaagat gcggcgcgct
    47821 acgccaacca gcgcgtgcag tttggcgagg ctattggtcg tttccagttg attcaggaaa
    47881 aattcgccca catggcgatc aaattaaact ccatgaaaaa catgctgtat gaagcagcgt
    47941 ggaaagcaga caacggcacc atcacctctg gcgatgcagc gatgtgcaaa tacttctgcg
    48001 ccaatgcggc atttgaagtg gtggatagcg caatgcaggt gctgggcggt gtcgggattg
    48061 cgggcaacca ccgcatcagc cgcttctggc gtgacctgcg tgtagaccgc gtttccggag
    48121 gatctgacga aatgcagatc ctgacgctgg gtcgtgcggt gctgaagcaa taccgctaag
    48181 tccgtgcgct gcctgatgcg acgctgacgc gtcttatcag gcctacggga gcacgaatgt
    48241 aagccggata aggcgtttac gccgcatccg gcagtcatgc gctacataaa tttttcagga
    48301 gctaataatg gatcatctac ccatgccgaa attcggaccg ctggccggat tgcgcgttgt
    48361 cttctccggt atcgaaatcg ccggaccgtt tgccggacaa atgttcgcag aatggggcgc
    48421 ggaagttatt tggatcgaga acgtcgcctg ggccgacacc attcgcgttc aaccgaacta
    48481 cccgcagctc tcccgccgca atttgcacgc gctgtcgtta aatattttca aagatgaagg
    48541 ccgcgaagcg tttctgaaat taatggaaac caccgatatc ttcatcgaag ccagtaaagg
    48601 tccggccttt gcccgtcgtg gcattaccga tgaagtactg tggcagcaca acccgaaact
    48661 ggttatcgct cacctgtccg gttttggtca gtacggcacc gaggagtaca ccaatcttcc
    48721 ggcctataac accatcgccc aggccttcag tggttacctg attcagaacg gtgatgttga
    48781 ccagccaatg cctgccttcc cgtataccgc cgattacttt tctggcctga ccgccaccac
    48841 ggcggcgctg gcagcactgc ataaagtgcg tgaaaccggt aaaggcgaaa gtatcgacat
    48901 cgccatgtat gaagtgatgc tgcgtatggg ccagtacttc atgatggatt acttcaacgg
    48961 cggcgaaatg tgcccgcgca tgagcaaagg taaagatccc tactacgccg gttgcggtct
    49021 gtataaatgt gccgacggct acatcgtgat ggaactggtg ggcattaccc aaattgaaga
    49081 gtgctttaaa gatattggcc tcgcacatct actcggtacg ccggaaatcc cggaaggcac
    49141 tcagcttatc caccgtatcg aatgccctta cggcccactg gtagaagaaa aactcgatgc
    49201 ctggctggcg gcacatacca tcgcagaagt taaagaacgc tttgccgaac tgaatatcgc
    49261 ctgtgccaaa gtactgaccg taccggaact ggaaagcaat ccacagtatg ttgcccgtga
    49321 atcaatcact cagtggcaaa cgatggatgg tcgcacctgc aaagggccga acatcatgcc
    49381 gaaattcaaa aataaccccg gacaaatctg gcgcggaatg ccctcacatg gcatggacac
    49441 ggctgccatt ttgaaaaata tcggctacag cgaaaacgac attcaggagt tggtcagcaa
    49501 aggtctggcc aaagttgagg actaaacgcg tggcgtcagt cggcttgcct gactagcgcc
    49561 cgcccaataa caaacgaaca aaaatggata gaggtgcaat ggatatcatt ggcggacaac
    49621 atctacgtca aatatgggac gatctggcgg acgtttacgg tcataaaacg gcgctgattt
    49681 gtgaatccag cggcggagtc gttaaccggt atagttatct tgagttaaat caggagatta
    49741 accgcacggc aaacctgttt tatacgctgg ggattcgcaa aggcaacaag gttgcactac
    49801 atctcgacaa ctgcccggaa tttatctttt gctggttcgg gctggcaaaa attggcgcga
    49861 ttatggtgcc gattaacgcc cgcctgttac gcgaggaaag cgagtggatc ttgcaaaata
    49921 gccaggcgtg cctgctggtg accagtgcgc aattttatcc tatgtatcaa cagattcagc
    49981 aggaagatgc cactcaattg cggcacattt gcctgacaga tgtggcactt cccgctgatg
    50041 atggagtgag ttcatttact caactgaaaa atcaacaacc tgccaccttg tgctatgcac
    50101 cgccgctatc gactgacgat acggcggaaa ttctcttcac ctccggcacc acctcccgac
    50161 cgaaaggtgt ggtgattacc cattacaacc tgcgcttcgc tggatattac tccgcctggc
    50221 agtgtgcact gcgtgacgat gacgtctaca tgacggtaat gcctgcgttt catatcgatt
    50281 gccagtgtac tgcggcgatg gcggcgtttt ctgccggggc cacctttgtg ctggtcgaga
    50341 aatacagcgc ccgcgccttc tggggacagg cgcagaagta ccgcgccacc attaccgaat
    50401 gtattccgat gatgatccgt acgttgatgg tgcagccgcc ttcagcgaac gatcggcaac
    50461 accgcctgcg ggaagtgatg ttttatctca acttgtcgga gcaggaaaaa gacgcatttt
    50521 gtgaacgctt cggcgttcgc ttgctgacgt cttatgggat gacggaaacc attgtgggca
    50581 ttatcggcga tcgccctagc gataaacgac gctggccgtc gattggtcgg gcggggtttt
    50641 gctacgaagc ggagatccgc gacgatcaca atcgcccgct cccggcaggt gagatcggtg
    50701 aaatctgtat taaaggcgta ccagggaaaa ccatcttcaa agagtatttt ctcaacccga
    50761 aagccactgc gaaagtgctg gaagccgacg gctggctaca taccggcgat accggatacc
    50821 gcgacgaaga aggctttttt tatttcgtcg atcgccgctg caatatgatc aaacgcggtg
    50881 gcgagaatgt ctcctgcgtg gagctggaaa atatcatcgc cacccatccg aaaattcagg
    50941 acatcgtggt tgtgggtatt aaagattcga ttcgcgatga agccatcaaa gcatttgtgg
    51001 tgctgaatga aggtgaaaca ttgagcgaag aggaattttt tcgcttctgc gaacaaaata
    51061 tggcgaaatt taaagtgccc tcttatctgg agatcagaaa agatctgcca cgtaattgct
    51121 cggggaaaat aattagaaag aatctgaaat aaatgaaacg tcagggaact accctgccag
    51181 ctaataatca cgccctaaaa caatatgcat tttttgcagg gatgttgtcg tccctaaaaa
    51241 agcaaaaatg gagaaaagga atgagtgaat cattacatct gacccgcaat ggatcaattc
    51301 tggaaattac ccttgatcgc ccaaaagcga atgctattga tgcaaaaacc agctttgaaa
    51361 tgggcgaagt atttctaaat ttccgtgacg atccgcaatt acgtgtcgcc attattaccg
    51421 gtgccggaga gaaattcttt tccacgggct gggatttaaa agcggcagca gaaggcgaag
    51481 caccggatgc tgactttggc ccgggtggtt ttgcgggatt aaccgaaatt ttcaatctcg
    51541 acaaaccagt tatcgcagct gtgaacggct acgcctttgg cggcggtttc gaactggcgc
    51601 tggcggcaga ttttattgtt tgtgccgata acgccagctt cgccctgccg gaagccaaac
    51661 tgggcatcgt tcctgacagc ggcggtgtgc tgcgtctgcc gaagatcctg ccgcctacca
    51721 tcgtcaatga aatggtgatg accggcagac gaatgggcgc agaagaggcg ctgcgttggg
    51781 ggatagtcaa ccgcgtggtt agccaggcgg aactgatgga taacgcccgc gaactggctc
    51841 agcagctggt taacagcgcc ccgctggcga ttgcggcgct gaaagagatc taccgcacca
    51901 ccagcgaaat gccggtagaa gaagcgtatc gctatattcg cagcggcgtg ttgaaacact
    51961 atccatcggt tctgcattcg gaagatgcca ttgaagggcc gctggcgttt gccgagaagc
    52021 gcgatccggt gtggaaagga cgttaacaac cgtgagctat tacgcctttg aggggttaat
    52081 tccggtggtt cacccgacgg cgtttgtcca tcccagtgcc gtcttgattg gcgatgtgat
    52141 tgtgggagcc ggtgtctaca tcggcccact cgcctcactg cgtggtgact acgggcggtt
    52201 gatcgtgcaa gcgggagcca atattcagga tggctgcatt atgcatggct actgcgacac
    52261 tgacaccatc gttggggaaa acggccatat cgggcatgga gcgatcctgc atggttgtgt
    52321 gattggtcgc gatgcattgg tcgggatgaa cagcgtaatt atggatggcg cggtcattgg
    52381 cgaagagagc attgttgccg caatgagctt tgtcaaagca ggctttagtg gcgagaaacg
    52441 ccagttgttg atgggtacgc cagcccgcgc tgtacgcagt gttagtgacg acgagttaca
    52501 ctggaaacgg ttgaatacca aagagtatca ggatcttgtc gggcgctgtc atgcatcgtt
    52561 acatgaaacg cagccactga ggcaaatgga ggaaaatcgc ccccgtttgc aggggacgac
    52621 ggatgtgacg cctaaacggt gatcttgccg gatgcgacct ggttgctgga taagatgcta
    52681 caacatcgta tctggcaagc aggacgcccg gtatcaacga gataaaatta acgacgcata
    52741 ctctttgaca acatcatctg ccacttctgc tcgcggttaa gttccgtgag cggaacggca
    52801 ggaatacgcg gtgtctttct tacgccgact aatttttccc gactgttatt gcgcaggcgc
    52861 gcgtaaattt gctcatcgat atcgaccact ttaaccagtc gctgacactg gcatccccgc
    52921 ccttccagct tattagggat catcttaact tcgcagctta tttctgtgac ttccgacaga
    52981 atataagtca gggtattaac cgccttgctg tgttcaatat cgaaatgaat agagatctct
    53041 cttgctatca cccaccgatt ttcagccatc atccattcgg cgattaacaa gtagagtggt
    53101 ttttcaacat atccttcaca catacgaact ccagtttcac aaattaaaag tctggctatg
    53161 actcgttttg aagcaattaa acaaggccat attaaaattg tggatatatc gatagtatgc
    53221 aacttcaccg tcgataagtg tgagctaaat cctgcttatg tcatcaaaaa tatcgattcc
    53281 ccaaaagatt tacttaatgg gcaaaaaaaa acggtcctca tcagagaacc gtattagtta
    53341 ttcgatcaaa ttagcggatg aaaaatatcc gccacgacac gctattattt gatctgtgcg
    53401 tgcatttcct gcaccgaaat tactttttca gtcgcatcgg cattcagcgc catcgcggta
    53461 gcgaaaccac cgttcagggt ggtgtcataa tgcactttat attgcagcgc actgcgacgg
    53521 atcacgcggg agtcttcaat cgcacgacgg cctgaggtgg tgttgatgat gtaggtatat
    53581 tcgccattct tgatacggtc ctgaatgtgc ggacggcctt catgcacctt gtttaccaga
    53641 cgcggattga tacccgcttc gcccagcaca atcgccgtgc cgtgggtcgc atccagctcg
    53701 aagccctgtt tcagcagttt tgccgccagg tccaccacgc gttctttatc gccttcgcgc
    53761 acggaaagca gcgcacgacc gtgtttcttc atggtggagt tgctgcccag ctgcgctttg
    53821 gcgaacgctt cagcgaaggt gcggcccacg cccatgactt ccccggtaga gcgcatttct
    53881 ggccctaaca gcgggtcaac gcctgggaat ttattgaacg gcagcaccac ttctttcacc
    53941 gagtagtacg gcggaataac ttctttggtt acgccctgct cagccagcga tttgccagcc
    54001 atcacacgcg ccgccacttt tgccagcggt acgccggtgg ctttggagac gaacggaaca
    54061 gtacgcgccg cacgcgggtt tacttcaatc aggtaaactt cgttgttttt caccgcaaac
    54121 tgcacgttca tcagaccgcg cacctgcaat tcgaaggcca gtttctgcac ctgctggcgc
    54181 atcacatcct gaatttcctg acttaaggtg tacgctggca gcgaacatgc ggagtcaccg
    54241 gagtgcacac ccgcctgctc gatgtgctcc atgatgccgc caatcagcac catttcgccg
    54301 tcgcagatgg catccacgtc aacttctact gcgtcatcaa ggaaatgatc cagcaacact
    54361 ggcgcatcgt tagacacgct aaccgccgtc tggaagtagc gacgcaggtc agcttcgtca
    54421 tagacgattt ccatcgcccg accgccgaga acgtaagacg gacgcaccac cagcgggtag
    54481 ccaatctctt ttgccttctc aactgccatt tcaatagcag taacggtggc gttcgccggt
    54541 tgtttcagtt tcagacggtc aaccgcatgc tggaagcgtt cacggtcttc cgcacggtcg
    54601 atagcatccg ggctggtgcc gataaccggt acgccagcgg cttccagtgc acgcgccagt
    54661 ttcagcgggg tctgaccgcc gtactgaacg ataacgcctt tcggcttctc aatacgcacg
    54721 atttccagca catcttccag agttaccggc tcgaagtaga ggcgatcgga agtgtcgtag
    54781 tcggtagaga cggtttccgg gttacagtta accataatgg tttcgtaacc gtcttcacgc
    54841 agcgccagcg aggcgtgtac gcagcagtag tcgaattcga taccctgacc gatacggttt
    54901 ggaccgccgc caagcaccat gattttttca cggtcggtag acggattcgc ttcgcactct
    54961 tcttcataag tggagtacat gtaagcggtg tcggtggcaa actctgccgc acaggtatcc
    55021 acgcgcttgt agaccgggtg caggtcatat tggtcacgca gcttacggat ttccgcttcg
    55081 cgtacgcccg ccagttttgc caagcgcgca tcggcaaagc ctttgcgttt cagctggcgc
    55141 aggaattcag cgttcaggcc agtgatgccc acttccgcca ctttctcttc cagacgcacc
    55201 agttcttcaa tctgtaccag gaaccagcgg tcaatgttag tcaggttgaa gacgccgtcc
    55261 acagacaggc ccgcgcggaa agcatcggcg atgtaccaga tacgctctgc gcctgcgtct
    55321 ttcagttcgc gacggatttt ggttaacgct tccgggtcat ccaggctcac tttcgggtcg
    55381 aagccagttg cgcccacttc caggccgcgc agcgcttttt gcagggattc ctgctgcgtg
    55441 cgaccaatcg ccatcacttc gccaaccgat ttcatctgag tggtcagacg gtcgttagca
    55501 ccggcgaatt tttcgaagtt gaagcgagga attttagtaa ccacatagtc gatggacggc
    55561 tcgaaggagg ccggagtacg tccgccagtg atgtcgttca tcagttcgtc tagggtgtaa
    55621 cccaccgcca gtttcgccgc cactttagca atcgggaaac cggtcgcttt cgacgccagc
    55681 gccgaagaac gggacacgcg tgggttcatt tcgataacaa tcaggcgacc gtttttcggg
    55741 ttcaccgcaa actggacatt ggaaccaccg gtttcaacgc cgatttcacg cagcaccgcc
    55801 atcgaggcgt tacgcatgat ttgatattct ttgtcggtca gcgtttgggc tggcgcgaca
    55861 gtgatggagt caccggtgtg gatgcccatc gcatcaaagt tttcgataga gcagacgatg
    55921 atgcagttgt cgtttttatc acgcaccact tccatctcgt actctttcca gccgatcagc
    55981 gactcatcaa tcagcaactc tttggtcgga gagagagcca gaccgcgggc gcaaatttct
    56041 tcaaactctt cacggttata agcgataccg ccgccgctac cgcccatggt aaaggatggg
    56101 cgaataatgc acgggaagcc cacgtcagcg gcaacagcca gcgcttcttc catcgtgtgt
    56161 gcgataccgg aacgcgcggt ttccagacca attttcttca tcgctacgtc gaaacgacgg
    56221 cggtcttctg ctttatcaat cgcatcggca gtggcaccaa tcatggtgac accgaactct
    56281 tccaacacgc cctgacgttc cagctccagc gcgcagttca gcgccgtctg accgcccatg
    56341 gttggcagca ccgcgtccgg gcgctctttt tcaataatct tgcgtaccac ttcccagtga
    56401 atcggctcga tgtaggtcgc atcggccatt tccgggtcgg tcatgatggt cgccgggttg
    56461 gagttcacca gaatgacgcg gtaaccctct tcgcgcaggg ctttacatgc ttgcgcgcca
    56521 gagtagtcaa actcacacgc ctgaccgata acaatcgggc ccgcacccag aatcaggata
    56581 ctttttatat ctgtacgttt tggcatggct cttttactcc tgattactta gcggttttac
    56641 ggtactgctc aattaacttg ataaagtggt cgaacaacgg cgcggcgtcg tgtggaccag
    56701 ggctggcttc agggtgaccc tggaagctga acgccggctt atcgatgcga tgaatgccct
    56761 gtaacgtacc gtcgaacagg gatttatgcg tgacacgcag gtttgcaggt aatgttgctt
    56821 catccaccgc aaaaccgtgg ttctgggcgg tgatcatcac cacgtttttc tccacatctt
    56881 taaccggatg gttgccgccg tggtgaccaa atttcatttt gacagtcttc gcaccgctcg
    56941 ccagcgccag cagctgatga ccgagacaga tgccgaatac cggaatatcg gtttcgagga
    57001 acttctggat ggcggtaatg gcgtaatcgc acggggccgg gtcgccagga ccgttggaga
    57061 ggaagatgcc gtctggattc attttcagca catcttccgc agaagtttgc gccggaacga
    57121 tggtcaggcg acagcctcta tccaccagca tccgcaggat gttgcgcttg gcaccaaaat
    57181 cataagccac gacgtggaac ggcagctcgt cttctttttt cgcttctggc aggccgccgg
    57241 tcaacgtcca gctcccttgt gtccagctat aggcttctgc ggtggtcact tcttttgcca
    57301 gatccatgcc gttcagacct gggaacgcgc gggctttttc taacgccagc gccgcatccg
    57361 ggttatcgcc tgcgataatg cagccattct gtgcgccttt ctcgcgcagt aaacgcgtca
    57421 gcttacgggt atcgatatcg gcaatcgcca cgatgttatg gcgcttcagg taagaagaga
    57481 ggtcttcggt attacggaag ttgctggcaa tcagcggcag gtcgcgaatc accagacctt
    57541 gtgcatgtac ctgagaagat tcttcatcgg cgtcattagt gccgacattg ccaatatggg
    57601 gataagtaag agtaacgatt tgacgagaat aggaaggatc agtgaggatt tcttgataac
    57661 cggtcattga agtattgaaa acgacttccc caaccgccga acctgttgcc cctatggccc
    57721 gaccgtgaaa ctgggttccg tcttccagaa ccaatagcgc tgacttaatc aaaacaccct
    57781 ccagagaata ctcactcact ttatttgcat attaattcac agtgatgacg taaatcaatg
    57841 caaatctgct taccgacgaa tttctggcaa acggcggcat tctggagata tgggcgctaa
    57901 aagtcaactt aatggcagga ttttttatat tattttgtgg ctcatgcatg ttttacaggc
    57961 attaagcgac aaaaacatca tctaacctgt tctggaaaac gcttgcgcag caagaagatt
    58021 gcatgaaata aaaacgagca ggaaaaagtg gaccaaatgg tcaaaattta cattaaaaca
    58081 acaaaaatag aagccgaaac atgtaatttt tggcagttat agaaaattta aaagcataac
    58141 accaaaaata aaagggctaa aaattaaagc cctttataat taataaatta aatgtgttac
    58201 ttttgcatca taataaacat tttgtggtta caaattattg agatcaagca catctcgcat
    58261 atcaaaaaga ccactttcct taccactcaa ccacaaagcc gatcttaccg caccgttagc
    58321 aaatgtcatg cggctggacg ccttatgggt gatctccaga cgctcgccaa tatcggcaaa
    58381 catcgcggta tgttcgccaa cgatgtcacc tgcacgcacg gtagcaaaac caatggtacc
    58441 tggcacacgt tcaccggtat ggccttcacg actgtagacc gcgcaatctt tcagatcttt
    58501 atcaagggcg tgggcgatcg cctctcccat tgccagtgcg gtgcctgacg gcgcatcaac
    58561 tttatgtcta tgatgtgctt caataatttc gatatcggtg tagtcaccca tcactttggc
    58621 tgctttctcc agcagcttaa gcatgacgtt aacgccaacg ctaaaattgg cggcaaagac
    58681 aatcgcaata tcggcggcgg cgtcacgaat tgcctgttta ccggcttcgt caaaccccgt
    58741 agtgccgatc accatccctt tgccatgctg ccgacaaaaa gcgagatggt tcagcgtacc
    58801 ttccggacgg gtaaaatcga taaacacatc aaaatcatct tttatcgcat caagactgct
    58861 ttgcacggta acgcctgttt tccctgcacc agccagctca ccggcgtcgc tgcccagtaa
    58921 agaagatcct tcgcgctcca gcgccgcgcc cagctgcacg ccttctaatg ccagtgccgc
    58981 ctgaatcaac tggcggccca tacgcccccc ggctcccgcg atggcaacgc ggatgtttgc
    59041 atcatgcata gctattctct tttgttaatt tgcatagacc gttttcagag taaccagcgc
    59101 atcgctacgc cgccagccaa aaacgctgat aattagaaat taatgataaa cagggaggga
    59161 tatcagtgaa aggcatgagt cgatgaatga ctgcatgccg ataacatgtg acaggttacg
    59221 gcaccagagc cagcacctca gccacccact gttggaagcc ttttacattc agatccaatg
    59281 ccacctgtac attggctggt ttgcccaggc aaccgtcgat atcaaccacc gtcgtgcctg
    59341 aggtaaattc gccttgagtt tctaccgcga caaaacacgg cttgagggtg aataagtccg
    59401 ggcgcaccag ccaggcgatg gcgcagagat cgtgcattcg cagaccgctt tgcatactgc
    59461 cgctacggta gtggctaaac agggcgtgga gcatttttcc ggtgcgattc aactccggta
    59521 aagtagcgag atagtcaggg gttaatattg cctgattggt gacatccaga ccgcacatga
    59581 caatttcgat gccgctacgg aagacacagg cagccgcttc tggatcggca gcgatattaa
    59641 actcggcatt tggtgtacag ttgccgcgac cagcagaacc gcccatgatc accagacgac
    59701 gaatatacgg tttacattcc gggcattgta aaagtaacag cgcaatattg gttaacgggc
    59761 cgatggccac cagggtaacg ggctctggtg cacgcatcag ggcatcccga atcgccagaa
    59821 acgccggtat cccgagtggc tttcggttgt gctcaacaaa gtcgtagcca gccattcccg
    59881 attcgccgtg cacagatgct gcatcacgcg gtgcgcgtac cagcggcaca gcggctccct
    59941 gggcgagcgg aatctccgca ttccagaaat gcagcagttg cagggcattg cgggtagttt
    60001 tctcaaccga gacattaccc gcgacggtgg tcatcagttg caggtcgagt tcgggggcaa
    60061 aaatcgcggc ggcaatggcg acggcatcgt caatgccggg gtcagtatcg aggaagatag
    60121 gtaaacgcat gttttcttca taaaaaatgc cggtaacaag accggcattt tcgcataact
    60181 taggctgcta atgacttaat cgacttcacg aatatcgaca cgcagctctt tcggcacttc
    60241 gaaaacaata ttttcttcac ggccttccag cggaatggct tcaccaccac ccaactgctg
    60301 caaacgtgcc accacattct gcaccagaat atccggagcc gatgcgcccg cagtcacgcc
    60361 gacgcattta acctctttca cccactcttc ctggatatct ttcgcatcgt caatcaaaaa
    60421 cgcgtgtttg cccatacgct gggccagctc cgccagacgg ttggagttgg aggagttttt
    60481 cgaaccgacc accaacacaa cttccgcctg ttctgccagg gcgcgtaccg cttcctgacg
    60541 gttagtcgtg gcgtagcaga tgtcatcttt gcgcggaccg acaattttcg ggaagcgttt
    60601 acgcagcgcg tcgatcacat cagacgtgtc atccaccgac agcgtggttt gggtcataaa
    60661 ggagagcttc tcttcgtttt tgaccgtcag tttccacaca tcgtctggcg attcgaccag
    60721 atacattccc ccttcggggt tgctgtactg ccccatcgtc ccttccactt ccgggtgccc
    60781 ggcgtggccg atgagaatag attcttcgcc acggcgactg gcgcgggcaa cttccatatg
    60841 cactttggtc accagcggac aggtggcatc aaacaccgtc aaatcgcggc ttttcgcttc
    60901 gttacgaacc gcctgagaaa caccgtgtgc ggagaaaatc aggatcgcgc cgtccggcac
    60961 ttcgctaatc tgctcaataa agatagcccc acgctcgcgc aagctatcga ccacgtagcg
    61021 gttatgcacc acttcgtgac ggacatatat cggtgcgccg taaatggcca gcgcgttttc
    61081 aacaatgctg atagcgcggt ctaccccggc acaaaaacca cgcgggttgg ccaacaggat
    61141 ctgcatgtta cgcctccagt gccggatcga tttccagcac ttcaatatca aaatgaacgg
    61201 tctgcccggc cagcggatgg ttgaaatcaa cggtaatgga gtcgccgtta atttcgcgga
    61261 tcacaccagg catctcactg ccatccattg cggtaaaaag catgattgcg ccaatttctg
    61321 gctcgcctgc atccataaat tcacggcggg agaagtactg aatcaggtcc ggtgacggca
    61381 cgccaaacgc cgcatctggc tccagcgaga aggtggtttt gtcgcccact ttcagcccca
    61441 gcaggtgttg ctccagccct tcagaaagag aagcatcgcc caggcggaac agcgccggtt
    61501 taccgttgtt gcgagtagac tcggcggtgg tgccatcgtc gagttttagc gtgaagtgca
    61561 ccaggacggc gctattgctc tgtacagatt cagacatgca ggtttgctct tatttttgag
    61621 gatttttgcc ggatgcggcc tacgatttcg tagcaatctg taggccgcat ccggcagggt
    61681 ttattattgt tttttcgctt tggaaggtaa aaacccttcc agcacgatca gtgccgcacc
    61741 gacacaaatg gcagtatcgg caaggttgaa ggtggcgaag tgccagtcgc cgacgtagaa
    61801 gtcgatcata tcgacaacga agccgtgcca caggcggtcg aacaggttgc ccagcgcgcc
    61861 gccaataatc agcgcgtaag cgatattgtt tagcttctgc gtggccttcg agcgatacat
    61921 catcactgcc aggagcacgc taataccaat cgcaataccg gcaaagaacc aacgctgcca
    61981 gccgccgcta tcggcaagga aactaaacgc cgcgccatag ttacgcgcat aatgcagatt
    62041 aagcgacggg aacagcggga ccgtatcccc cagagcaaag ttctggagga tcaggtattt
    62101 gctgcccaga tcgataatca gcacgactac caccagccac agccagcgta gccctgttga
    62161 acagatcgat tgactcatca ggcaaactta cgtttttcac cgtcaccggc gacgttgctg
    62221 acacagcggc cgcagatttc tgcgtgttcc gccaccttgc cgacatcctg ggtgtagtgc
    62281 cagcagcgtg ggcacttctc accttcggct ttactcaacg cgactttcag ccctttgagt
    62341 acttcgctct gctgagcatc agcaggtgcg tcgttatagt ctgcaacggt agcgccggag
    62401 gtcaacagga caaatcgtaa ttcatcgccc agcgcggtca gtttcgccgc cagttccggt
    62461 tctgcataca aggttactgc cgcttccagc gagccaccca ctttcttgtc ggcacgcgct
    62521 tgctcaatga ctttgttcac ttcgccacgc actttcaaca gctcgtccca gaacgcatcg
    62581 ttcattgctt cactgtctgc cagaccaaac aggccttcgt accactcacc ggtgaagacg
    62641 tatttttcac gttcgcccgg cagatagccc cacacttcat cagcggtgaa ggagaggatt
    62701 ggtgccatcc agcgcaccag cgcttcagcg atgtgataca gcgcagtctg gcagctacga
    62761 cgcgccacgc tatccgcttt ggcggtgtac tgacggtctt tgatgatgtc gaggtagaag
    62821 gaacccatct caacggagca gaagcgcatc agacgctgta ccacttcgtg gaaatcgtat
    62881 gcttcgtacg ccttgaggat gtcttcctgt gccgctttcg cacaacctac ggcccagcga
    62941 tccagtacca ccatctcttc cggtttcacc atatcttttg ctggatcaaa accattcagg
    63001 tttgccagca ggaagcgcgc ggtgttacgg atacgacgat agctatcggc agcacgtttc
    63061 aggatctcgt cagaaacggc catttcaccg gtgtagtcgg ttgatgccac ccacagacgc
    63121 agaatatccg cgcccagttt gttcatcaca tcctgcggcg aaacggtgtt gccgatggat
    63181 ttagacatct tgcgaccctg accatccacg gtaaagccgt gggtcagtac ctgacgatac
    63241 ggcgctttgc ctttcatcgc ggtggagatc attagggaag acatgaacca accacggtgc
    63301 tggtcagaac cttccagata catgtccgct gcgtgaccgg caaattccgg acgcacgtca
    63361 acaacagaag agtgggtaga tccggagtca aaccatacgt cgagagtatc cggcactttc
    63421 acatactggt cagcttcgtc gccgaggatc tctttcgcat cgagatccca ccacgcctgg
    63481 atgccgtcaa cttcaacgcg tttagccact tcttccatca gttcgagggt acgcggatgc
    63541 agctcttccg tgtctttgtg cacgaacagt gacatcggta cgccccaggt gcgctgacgg
    63601 gagatacacc agtcaggacg gttagcaacc atcgactcga tacgcgcctg gccccagtcc
    63661 gggagccact gcacgccttt gatctctttc agtgactgcg cacgcagacc tttctgatcc
    63721 atgctgacga accactgcgg tgtcgcgcgg aagatgatcg gcgttttgtg acgccagcag
    63781 cacggatagc tgtgctgcat tttctcaacg tgcagcagcg cgcctttttc ctgcagcagc
    63841 gcaacgacga tgtcgttcgc tttgaagacg ttcacgccat ccagcgtcgg ataagtgccc
    63901 ggcagataag tgccgtccgg gccaaccggg ttagcggttt ccaggccgta tttctgaccg
    63961 atcacatagt cgtccgggcc gtggccaggc gcggtgtgaa cggcaccggt accggcatcc
    64021 agggtaacgt gatcgccgag gattgccgga acgtcgaagc ccataaacgg atgggtaaag
    64081 cgcagcagct caagctctgc accttttacc gtgccgagaa tggtgtaatc ggtcacgccg
    64141 atacgctgca ttacgctttc aaccagatct ttcgccagaa tcacggcctg accgtcgatc
    64201 tgcaccagcg catagtcgaa atcaggtgca atagagattg cgcggttcgc aggcagagtc
    64261 cacggcgtag tggtccagat caccagcgag attgggccgt taacgttgct tacgccaaat
    64321 ttcgctttca gcgcatcctg atcgaccgcc tggaaagcga cgtcaatgga cggagaagtt
    64381 ttgtcgtaat actcaacttc cgcttctgcc agtgcagaac ggcagtctac gcaccagtgc
    64441 accggcttcg cgcctttgtg caggtgacca ttgccgatga ttttgcccag cgcgcggatg
    64501 atgttggctt cagttttgaa gtccatagtc agatacgggt gcgaccagtc gcccagcacg
    64561 cccagacgga taaagtcttt gcgttgaccg tcaacctggg tcgccgcgta ttcgcggcac
    64621 ttggcgcgga actcggcggc ggtgaatttc tcacccggct taccgtattc ttgctctact
    64681 ttcagctcga tcggcagacc gtggcagtcc cagccaggca catacggcga gtcataaccg
    64741 gaaagccctt tggacttcac gataatgtct ttcagaatct tgttaaccga gtgaccaata
    64801 tgaatgctgc cattcgcata aggagggcca tcatgcagaa tgaaggtttt tttgcctttt
    64861 ttagccgcac ggatgatgcc gtacagatca tcatcagtcc aacgcgccag cattcccggt
    64921 tcgcgcttgg cgagatcgcc acgcatcggg aaccctgttt ccggcaaatt cagggttgat
    64981 ttatagtcac tcatcagatt ctcggttccg tgtttcggtt tgattacata acaggcttaa
    65041 gccggttttg ttagcccaaa aaattcgcgg gcggttaatt catcacgcgc aatctgcgct
    65101 ttcagttcgt ccagcgacgc aaatcgctgc tcattgcgta tttttttacg cagcactact
    65161 tgtatatggc gaccgtaaag gtccattgca acatctaaca aatgcacttc cagctgctgg
    65221 cgaataccgg caaccgttgg gcgtgttccg atgtttgcca cgccgggtaa cggcttttca
    65281 ccgaggccca gcacttctac cgcataaacc cctttcaccg gggaaacctg acggcgtagc
    65341 ggtacattcg ccgtcgggaa acctatagtg cgccctaatt catcaccgtg gactacacgc
    65401 ccggagatgg caaacgggtg ccccagtaaa ctttctgcca gagccagatt gtcatcagcc
    65461 agcgcctgac gcacggcggt gctgctgata cgcacaccac cttcgcaaaa agtttgcgta
    65521 ctggtgatat cgaagccgta ttccatgcca gctttctgta ataacaagaa atcgccttca
    65581 cgaccagcgc caaagcggaa atcatcacct acggcaagaa attttacgcg caaatgcttc
    65641 accaaaagat cgctgatgaa attttgcgcg gttaacgccg caaaacgcct gtcgaaacgc
    65701 acgcacagca cgtaatcaac gccacactct gcaaggtaac gcagtttttc ccgcagccgg
    65761 gtcagtcttg ccggggcttt atcggtagca aacagttcca gtggttgagg ttcaaaaagc
    65821 atcaccatca ccggtaagtt gcgcttgcgc ccttcttcct gcaagccctg taacagcgcg
    65881 cgatgaccgc gatgcacgcc gtcgaaatta ccaatagtca gcacacaccc ttcttgcggg
    65941 gcctggctga gattatgtat gccgcgtatc agcttcatgt ctggctcaaa acagtgaaaa
    66001 tcgtccgagt ataccttgta cagcggtcaa ggttaaccgg cgattgagta ccgaaatcag
    66061 aaagaagtgg cttttcattg ccatggagca aatcacggga agaaactgac cgcctgctgc
    66121 aatttttatc gcggaaaagc tgtattcaca ccccgcaagc tggtagaatc ctgcgccatc
    66181 actacgtaac gagtgccggc acattaacgg cgcttatttg cacaaatcca ttgacaaaag
    66241 aaggctaaaa gggcatattc ctcggccttt gaattgtcca tatagaacac atttgggagt
    66301 tggaccttgg ctaatatcaa atcagctaag aagcgcgcca ttcagtctga aaaggctcgt
    66361 aagcacaacg caagccgtcg ctctatgatg cgtactttca tcaagaaagt atacgcagct
    66421 atcgaagctg gcgacaaagc tgctgcacag aaagcattta acgaaatgca accgatcgtg
    66481 gaccgtcagg ctgctaaagg tctgatccac aaaaacaaag ctgcacgtca taaggctaac
    66541 ctgactgcac agatcaacaa actggcttaa tcgccaattt gctgaagctt tgtgaaaaag
    66601 cccgcgcaag cgggtttttt tatgcctgct gcttttgtga tgcgtgagag aacacactgc
    66661 cggttgccag atagtcaaaa gagacatgcc gttgtactat ctgaactctt cgtggcttaa
    66721 gaatcgtaga accatcggct gatacattct gaacaagaat atctactcaa tctccagact
    66781 cccccacaaa gaatatggat attgtgatac acattgaggt attaaactta agaccactct
    66841 atttttgaat taaattccta tatactttaa ccattcataa atatctcctc attagacctt
//
LOCUS       DAJVPP010000006        65547 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVPP010000006 DAJVPP010000000
VERSION     DAJVPP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.006"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            135..750
                     /locus_tag="OQM34_000400"
                     /pseudo
     CDS             135..750
                     /locus_tag="OQM34_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            853..1176
                     /locus_tag="OQM34_000401"
     CDS             853..1176
                     /locus_tag="OQM34_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCS1450976.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1243..2700)
                     /gene="nuoN"
                     /locus_tag="OQM34_000402"
     CDS             complement(1243..2700)
                     /gene="nuoN"
                     /locus_tag="OQM34_000402"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1450977.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2707..4236)
                     /gene="nuoM"
                     /locus_tag="OQM34_000403"
     CDS             complement(2707..4236)
                     /gene="nuoM"
                     /locus_tag="OQM34_000403"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1450978.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4400..6241)
                     /gene="nuoL"
                     /locus_tag="OQM34_000404"
     CDS             complement(4400..6241)
                     /gene="nuoL"
                     /locus_tag="OQM34_000404"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1450979.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6238..6540)
                     /gene="nuoK"
                     /locus_tag="OQM34_000405"
     CDS             complement(6238..6540)
                     /gene="nuoK"
                     /locus_tag="OQM34_000405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1450980.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6537..7091)
                     /gene="nuoJ"
                     /locus_tag="OQM34_000406"
     CDS             complement(6537..7091)
                     /gene="nuoJ"
                     /locus_tag="OQM34_000406"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1450981.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7103..7645)
                     /gene="nuoI"
                     /locus_tag="OQM34_000407"
     CDS             complement(7103..7645)
                     /gene="nuoI"
                     /locus_tag="OQM34_000407"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1450982.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7660..8637)
                     /gene="nuoH"
                     /locus_tag="OQM34_000408"
     CDS             complement(7660..8637)
                     /gene="nuoH"
                     /locus_tag="OQM34_000408"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1450983.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8634..11360)
                     /gene="nuoG"
                     /locus_tag="OQM34_000409"
     CDS             complement(8634..11360)
                     /gene="nuoG"
                     /locus_tag="OQM34_000409"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1450984.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11413..12750)
                     /gene="nuoF"
                     /locus_tag="OQM34_000410"
     CDS             complement(11413..12750)
                     /gene="nuoF"
                     /locus_tag="OQM34_000410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1450985.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12747..13247)
                     /gene="nuoE"
                     /locus_tag="OQM34_000411"
     CDS             complement(12747..13247)
                     /gene="nuoE"
                     /locus_tag="OQM34_000411"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1450986.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13250..15052)
                     /gene="nuoC"
                     /locus_tag="OQM34_000412"
     CDS             complement(13250..15052)
                     /gene="nuoC"
                     /locus_tag="OQM34_000412"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1450987.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15146..15808)
                     /gene="nuoB"
                     /locus_tag="OQM34_000413"
     CDS             complement(15146..15808)
                     /gene="nuoB"
                     /locus_tag="OQM34_000413"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1450988.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15824..16267)
                     /gene="nuoA"
                     /locus_tag="OQM34_000414"
     CDS             complement(15824..16267)
                     /gene="nuoA"
                     /locus_tag="OQM34_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1450989.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16898..17836)
                     /gene="lrhA"
                     /locus_tag="OQM34_000415"
     CDS             complement(16898..17836)
                     /gene="lrhA"
                     /locus_tag="OQM34_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1450990.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18756..19973
                     /gene="alaA"
                     /locus_tag="OQM34_000416"
     CDS             18756..19973
                     /gene="alaA"
                     /locus_tag="OQM34_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1450991.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20057..20656
                     /gene="yfbR"
                     /locus_tag="OQM34_000417"
     CDS             20057..20656
                     /gene="yfbR"
                     /locus_tag="OQM34_000417"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1450992.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20715..22547)
                     /locus_tag="OQM34_000418"
     CDS             complement(20715..22547)
                     /locus_tag="OQM34_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1450993.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22634..23284)
                     /gene="hxpA"
                     /locus_tag="OQM34_000419"
     CDS             complement(22634..23284)
                     /gene="hxpA"
                     /locus_tag="OQM34_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCS1450994.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23295..23789)
                     /locus_tag="OQM34_000420"
     CDS             complement(23295..23789)
                     /locus_tag="OQM34_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1450995.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23872..24327)
                     /locus_tag="OQM34_000421"
     CDS             complement(23872..24327)
                     /locus_tag="OQM34_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1450996.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24665..25867
                     /gene="ackA"
                     /locus_tag="OQM34_000422"
     CDS             24665..25867
                     /gene="ackA"
                     /locus_tag="OQM34_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1450997.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25942..28086
                     /gene="pta"
                     /locus_tag="OQM34_000423"
     CDS             25942..28086
                     /gene="pta"
                     /locus_tag="OQM34_000423"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1450998.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDTPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28276..29796
                     /gene="yfcC"
                     /locus_tag="OQM34_000424"
     CDS             28276..29796
                     /gene="yfcC"
                     /locus_tag="OQM34_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1450999.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29829..30371)
                     /gene="yfcD"
                     /locus_tag="OQM34_000425"
     CDS             complement(29829..30371)
                     /gene="yfcD"
                     /locus_tag="OQM34_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1451000.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30429..30980)
                     /gene="yfcE"
                     /locus_tag="OQM34_000426"
     CDS             complement(30429..30980)
                     /gene="yfcE"
                     /locus_tag="OQM34_000426"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1451001.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31036..31680)
                     /gene="yfcF"
                     /locus_tag="OQM34_000427"
     CDS             complement(31036..31680)
                     /gene="yfcF"
                     /locus_tag="OQM34_000427"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1451002.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31816..32463
                     /gene="yfcG"
                     /locus_tag="OQM34_000428"
     CDS             31816..32463
                     /gene="yfcG"
                     /locus_tag="OQM34_000428"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1451003.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPPDGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32520..32882
                     /gene="folX"
                     /locus_tag="OQM34_000429"
     CDS             32520..32882
                     /gene="folX"
                     /locus_tag="OQM34_000429"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1451004.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32903..33796
                     /locus_tag="OQM34_000430"
     CDS             32903..33796
                     /locus_tag="OQM34_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1451005.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33844..34734)
                     /gene="rpnB"
                     /locus_tag="OQM34_000431"
     CDS             complement(33844..34734)
                     /gene="rpnB"
                     /locus_tag="OQM34_000431"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1451006.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34931..35704)
                     /gene="hisP"
                     /locus_tag="OQM34_000432"
     CDS             complement(34931..35704)
                     /gene="hisP"
                     /locus_tag="OQM34_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS1451007.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTCLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35712..36428)
                     /locus_tag="OQM34_000433"
     CDS             complement(35712..36428)
                     /locus_tag="OQM34_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1451008.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTVLAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36425..37111)
                     /gene="hisQ"
                     /locus_tag="OQM34_000434"
     CDS             complement(36425..37111)
                     /gene="hisQ"
                     /locus_tag="OQM34_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1451009.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37201..37983)
                     /gene="hisJ"
                     /locus_tag="OQM34_000435"
     CDS             complement(37201..37983)
                     /gene="hisJ"
                     /locus_tag="OQM34_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1451010.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38204..38986)
                     /gene="argT"
                     /locus_tag="OQM34_000436"
                     /pseudo
     CDS             complement(38204..38986)
                     /gene="argT"
                     /locus_tag="OQM34_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
     gene            complement(39252..39821)
                     /gene="ubiX"
                     /locus_tag="OQM34_000437"
     CDS             complement(39252..39821)
                     /gene="ubiX"
                     /locus_tag="OQM34_000437"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1451011.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39916..41433)
                     /gene="purF"
                     /locus_tag="OQM34_000438"
     CDS             complement(39916..41433)
                     /gene="purF"
                     /locus_tag="OQM34_000438"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1451012.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41470..41958)
                     /gene="cvpA"
                     /locus_tag="OQM34_000439"
     CDS             complement(41470..41958)
                     /gene="cvpA"
                     /locus_tag="OQM34_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1451013.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42217..42879)
                     /gene="dedD"
                     /locus_tag="OQM34_000440"
     CDS             complement(42217..42879)
                     /gene="dedD"
                     /locus_tag="OQM34_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1451014.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42869..44137)
                     /gene="folC"
                     /locus_tag="OQM34_000441"
     CDS             complement(42869..44137)
                     /gene="folC"
                     /locus_tag="OQM34_000441"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1451015.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44207..45121)
                     /gene="accD"
                     /locus_tag="OQM34_000442"
     CDS             complement(44207..45121)
                     /gene="accD"
                     /locus_tag="OQM34_000442"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1451016.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45277..45936)
                     /locus_tag="OQM34_000443"
     CDS             complement(45277..45936)
                     /locus_tag="OQM34_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1451017.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46019..46831)
                     /gene="truA"
                     /locus_tag="OQM34_000444"
     CDS             complement(46019..46831)
                     /gene="truA"
                     /locus_tag="OQM34_000444"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1451018.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46831..47844)
                     /locus_tag="OQM34_000445"
     CDS             complement(46831..47844)
                     /locus_tag="OQM34_000445"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1451019.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47910..49046)
                     /gene="pdxB"
                     /locus_tag="OQM34_000446"
     CDS             complement(47910..49046)
                     /gene="pdxB"
                     /locus_tag="OQM34_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1451020.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49145..50140
                     /gene="flk"
                     /locus_tag="OQM34_000447"
     CDS             49145..50140
                     /gene="flk"
                     /locus_tag="OQM34_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1451021.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50137..51315)
                     /locus_tag="OQM34_000448"
     CDS             complement(50137..51315)
                     /locus_tag="OQM34_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1451022.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51570..52811)
                     /gene="fabB"
                     /locus_tag="OQM34_000449"
     CDS             complement(51570..52811)
                     /gene="fabB"
                     /locus_tag="OQM34_000449"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1451023.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            52970..54976
                     /gene="mnmC"
                     /locus_tag="OQM34_000450"
     CDS             52970..54976
                     /gene="mnmC"
                     /locus_tag="OQM34_000450"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1451024.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55097..55375)
                     /locus_tag="OQM34_000451"
     CDS             complement(55097..55375)
                     /locus_tag="OQM34_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1451025.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55409..55957)
                     /gene="epmC"
                     /locus_tag="OQM34_000452"
     CDS             complement(55409..55957)
                     /gene="epmC"
                     /locus_tag="OQM34_000452"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1451026.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55957..56766)
                     /locus_tag="OQM34_000453"
     CDS             complement(55957..56766)
                     /locus_tag="OQM34_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1451027.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56766..57590)
                     /gene="mepA"
                     /locus_tag="OQM34_000454"
     CDS             complement(56766..57590)
                     /gene="mepA"
                     /locus_tag="OQM34_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1451028.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57594..58679)
                     /gene="aroC"
                     /locus_tag="OQM34_000455"
     CDS             complement(57594..58679)
                     /gene="aroC"
                     /locus_tag="OQM34_000455"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1451029.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58714..59646)
                     /gene="prmB"
                     /locus_tag="OQM34_000456"
     CDS             complement(58714..59646)
                     /gene="prmB"
                     /locus_tag="OQM34_000456"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1451030.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59812..60363
                     /gene="smrB"
                     /locus_tag="OQM34_000457"
     CDS             59812..60363
                     /gene="smrB"
                     /locus_tag="OQM34_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1451031.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMYGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60467..61357)
                     /locus_tag="OQM34_000458"
     CDS             complement(60467..61357)
                     /locus_tag="OQM34_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1451032.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61344..61868)
                     /locus_tag="OQM34_000459"
     CDS             complement(61344..61868)
                     /locus_tag="OQM34_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1451033.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61865..62335)
                     /locus_tag="OQM34_000460"
     CDS             complement(61865..62335)
                     /locus_tag="OQM34_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1451034.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62332..62880)
                     /locus_tag="OQM34_000461"
     CDS             complement(62332..62880)
                     /locus_tag="OQM34_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1451035.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62885..63607)
                     /locus_tag="OQM34_000462"
                     /pseudo
     CDS             complement(62885..63607)
                     /locus_tag="OQM34_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63627..64349)
                     /locus_tag="OQM34_000463"
     CDS             complement(63627..64349)
                     /locus_tag="OQM34_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1451036.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64409..>65547)
                     /gene="yfcU"
                     /locus_tag="OQM34_000464"
     CDS             complement(64409..>65547)
                     /gene="yfcU"
                     /locus_tag="OQM34_000464"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCS1451037.1"
                     /translation="RYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGGSVSTDDQML
                     PPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSVSGTLHIRIE
                     EQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGAEASWGIANG
                     WSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFR
                     VSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEMYTATYNQNF
                     RDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYRYEYDNQADK
                     GMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
BASE COUNT        16326 a        17806 c        16359 g        15056 t
ORIGIN      
        1 cccactgtaa aaaacacttt ttgtatacct ctaccaagcg taataaaata aacgctcata
       61 attcattata aataataatg aaataacgcc ttttattgat aatttacata tgagaaaaat
      121 tttccttttg ttatattatt tctgttttca ctccctctga ttatttttac caaccctggg
      181 tcactgcatt atcgccaacg ccccgacacg taagtcctga gcaattagaa aaaacagttc
      241 gttatcttac acaaactgtg catccacgta atgccggcaa tattgataat ctgaataggt
      301 ccgcagagta tataaaagag ttctttgtca gtagcggtgc cagagttacc gcgcagggcg
      361 tccccgattg gctattacga ctctgcgcct ggaagccaga attaccctta cccggcaatg
      421 tcctggcttt atcccgatcg gggagacttt attgctgtgg tcggcagaat gcaggatatc
      481 aacgccgttc gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct
      541 atgaatgccc cagggtttat tcccggtatt gatttttctg atcacctcaa ttattggcaa
      601 cacgatattc ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac
      661 ttgcccggtg ataccgcaga cagattgaat tatcagaaaa tggctcaggt ggtagatggt
      721 gttataacct tgttatacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca
      781 gcgacatgta cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg
      841 gaggaaaaat aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac
      901 caactgcttg agtctactga aaaacctttg atggataata tacatgacac gctttcagga
      961 ttacgccgac tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga
     1021 ctcaatgaaa tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc
     1081 tttgcctcga tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga
     1141 caagggaatc atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac
     1201 cgccgaacat gtcggcggct ttctgactta caaactaaca gtttacatca gcggcattgc
     1261 taaccgcaca atactaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc
     1321 agaaatcagc accacgatac cgcccgcgct gtactgccag tttgacggtg catcgcgacc
     1381 cggttgttcc ggtgcgtgca ggtacaggct caccgccacg cgcaggtagt agtagaggcc
     1441 gattgccgaa ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac
     1501 cgccagcacg tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag
     1561 catcatcacc gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga
     1621 gaacagggaa tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa
     1681 cgcgccgagg ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat
     1741 ctcgccggtt tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga
     1801 gtaaccgagc agacgtttga tgttggtctg gctcaacgcc atcaggttac cgaagatgat
     1861 ggaggcaaag gcgataatcg ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc
     1921 gtagaggaac agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa
     1981 agtggaaacc ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac
     2041 cagagagagt ttgaagccga ggccaacaat catcatgccg aaacctgcca gcaacagcgg
     2101 ctcgttgagc ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga
     2161 ctgcgcatac accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat
     2221 ggtgtatttg atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc
     2281 aaacagcggc aaagagatca gttcaatacc gaggaacaga gacgccagat ggtttgcatt
     2341 cgccagcagg atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt
     2401 gtcgttatag ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa
     2461 taccagcccg gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat
     2521 agcgcccgcc tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga
     2581 gagcgtagcg ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac
     2641 cgtcaagccg acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat
     2701 ggcgatttac ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat
     2761 cgcggagtgc gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac
     2821 caccagcaac aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc
     2881 aatctggctt ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg aataaacaga
     2941 tgcaaagacc agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc
     3001 gaacagaatc ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac
     3061 cgcaaagaac agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat
     3121 gcgcatgtcg cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc
     3181 cgccgacaag ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc
     3241 ggtgtagatt gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg
     3301 tttgatatcg gtctgagcga aggtcatcca cgcaccgtag aagatgccga taacacccag
     3361 ccacatagcg attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg
     3421 caacaaaccg taagcggcgg ttttcagcaa gatccccgcg aggtccacgg aaccggcggt
     3481 cggagcctgg gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttgac
     3541 tgcgaaggcg atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt
     3601 attcagcagc tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac
     3661 cagcgccagg atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt
     3721 ggttgccgcc gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag
     3781 gaagtacatc ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc
     3841 aaggaacacg ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg
     3901 atatttttcg atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag
     3961 cacgaccatc agcagcgaca gaccgtcaat ggcgagatga atagagatac caaaacgcgg
     4021 gatccacggc atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga
     4081 ataaccgccc tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag
     4141 cgcgatccag cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc
     4201 aataaaggga attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag
     4261 tcccgtcagg accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg
     4321 gtagtgtcgg atgcgccgcg aaggcagctt atccgaccta cgttcttata tgcccagggg
     4381 caatcccaca atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac
     4441 cgatgctcat ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt
     4501 tacctgcaaa gcgggaaaga accgccggga tgttcatcat tgagttcagc ggatcgcgtt
     4561 tcagcaacca ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa
     4621 agccccaagc gttgtaccac caggtactca gcaggcggcc cggcgcactg ttggcgatgg
     4681 aggtcaccag agaacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg
     4741 cgaccacgcc agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt
     4801 gcggaagcac gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca
     4861 gcagcacaat cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt
     4921 cttttccgtg gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac
     4981 cgaccagacc tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct
     5041 catccttact gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca
     5101 ccaggaagca gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt
     5161 tctgttcgtg atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa
     5221 agaacgcgtg agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga
     5281 ggaacatgta gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct
     5341 gtaccagcgc ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat
     5401 gcagaacttc cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg
     5461 cggttaccat cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt
     5521 cagcaagcca tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca
     5581 tcagcgtcgc ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca
     5641 ccatttcgcg gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga
     5701 ggaaaacgtc acccacacgg gtcacgacga acgctttcat tgctgctgcg ccattcttcg
     5761 gatcggtgta atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga
     5821 ggtacatcag cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt
     5881 tggtgtaggc gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca
     5941 tgtgaataag gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca
     6001 gcaccaggtt aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc
     6061 tgtatgcctg ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt accagcgccg
     6121 ccaggcccac agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg
     6181 agaacgccag caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca
     6241 tccgcgcatc tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag
     6301 cagcagcgca aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat
     6361 cacctgaccg tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc
     6421 gttaatcatg atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc
     6481 ggttaagcca agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat
     6541 gcgtgctcct ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc
     6601 gcacgctctt cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa
     6661 gccagttcca ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg
     6721 attggcgtac cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac aatcaccacc
     6781 agcatgatgg ccgacaaaat tgccgggcca atccacacct gcggtttcag ccactggcgt
     6841 tcctgttcga tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc
     6901 ataatggcac ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcctagtgag
     6961 aagaacaccc cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga
     7021 ttggtatggg tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa
     7081 gcgaactcca ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt
     7141 cgttctctgc ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt
     7201 tatattccgg gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat
     7261 cctggcgctt gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac
     7321 aggcttcttc gcacagacca cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg
     7381 gataccagcg accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg
     7441 ctaccgcaca gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa
     7501 cgatacgacc acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt
     7561 cgcgtttggc gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac
     7621 ctactaacaa ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat
     7681 gacagccgcc gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa
     7741 ggacattacc cggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat
     7801 cataaagaac gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca
     7861 gccaccgaag aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc
     7921 cacgaagaac agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc
     7981 ctgctcggct tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc
     8041 aaaggtaata aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac
     8101 gatgtcggtc atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag
     8161 gaacacttcg tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt
     8221 gttgttactt gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag
     8281 gaagaacaaa atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat
     8341 tgcaaaggcc agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg
     8401 atccgagaat ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag
     8461 ctggagcgaa ccgccccagc caacacggtt aggtccataa cggttctgga acagacccag
     8521 caggcgacgt tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatcac
     8581 caccgctttg aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt
     8641 tgtgcctcct tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata
     8701 cccatcggca agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg
     8761 accgtgttgc catcgtaact aaaggagacg cgtgtacctg cgttcacacc caacttcgcg
     8821 gcatccgctg ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca
     8881 cgctgtgaca attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg
     8941 tcctgcggct ggaagcgtgc cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt
     9001 tcaaacagac gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat
     9061 ttgttccacg cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg
     9121 tgcgcggtcg gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc
     9181 ggctgacgcg gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg
     9241 tgcggttcac gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct
     9301 ttgatacccg ccagttccgg gattttcgcc acaacagcgt caattacatg gtcgagctgc
     9361 gtccagtcca cttcacggct cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt
     9421 tccagcatga cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg
     9481 gcgcgacctt cattattaat taccgtaccg tcgctttcag caaagctggc agaggaaagc
     9541 accagatggg cgttttccat gatcgctgtg cgttgatgat caaccaccat caccagcggc
     9601 gctttagcca gcgcagcatt cacgcgggta gcagaagcgt aacgatgcag atcgttttcc
     9661 aacaccacca ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa
     9721 ccgccaccca taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca
     9781 acgtcagcgc cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct
     9841 atgctaccgg cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc
     9901 acgatgacgt cgattttgtt ttgcaactca ggttcgatac cgtcaaccgc tggtgcggag
     9961 ttatccagcg catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg
    10021 taagtccacg ccgcgatatc atccagacga gtgtcatcaa cgttggtaac aaacagcgga
    10081 tgcttcgcac gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt
    10141 gctgcagcca tttcgcgcgc tttacctttc accgcctgac gcactgccag tgcgacgcgc
    10201 gcgccggtct gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg
    10261 cgcagagccg gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt
    10321 tcctgctcac cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc
    10381 gcaaagttgc tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga
    10441 cgcagaatat ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg
    10501 ccacgacgct gtactggctg acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa
    10561 cgaccacggt cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga
    10621 cgcagttcgc cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag
    10681 atgctcggcg caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg
    10741 gtaaatacgc cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg
    10801 ccgtcttccg ggcgaccgaa gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta
    10861 ccgtcagcgt aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt
    10921 tcgtgagaga tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag
    10981 cgacggaagc tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct
    11041 tcacataccg gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg
    11101 aactgtttcg cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata
    11161 caggacatca ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac
    11221 tggcggcaag caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg
    11281 cccagagaca gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg
    11341 tctacatgaa ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta
    11401 atcgaaattc ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg
    11461 ggtattgctg aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc
    11521 gctctgtaac ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa
    11581 gaatcgacac agttgctcaa gtgtttcgat atcgcccggc tggccttcgc cacgctccag
    11641 cgcacgcaga attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca
    11701 ggactcacgg gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg
    11761 gtcaaccgcc atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa
    11821 ttccatcggc agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg
    11881 ccaggcttta aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg
    11941 tgcggtggtg ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc
    12001 catcagcttg gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt
    12061 agcgaggatc gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc
    12121 ccatacgccg gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc
    12181 cagggagttg attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac
    12241 gaacagttcg aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt
    12301 agcttcggca atggcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa
    12361 gatgtagcca cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag
    12421 gtgcggcagt tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc
    12481 gttacacagc aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag
    12541 gccagtcgaa aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac
    12601 gatttcgtcc ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct
    12661 gcggtattcg tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg
    12721 cgtttcggga gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct
    12781 tccggggtca gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag
    12841 ttccccaggc agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt
    12901 ttgatgttca gctttttctc gagcgccgcc tgaataccct gatacccgtt gatatgacag
    12961 accacgctgt cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctgactg
    13021 tagaacgttg ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg
    13081 tggatcgcac catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg
    13141 gacgccgcac gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc
    13201 gcactcagct caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca
    13261 catctgacat aacaaaatcg atactgccca gataaacaat caggtcagac accaggctgc
    13321 cgcggatcgc cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc
    13381 gataactcat ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct
    13441 caatcatctg gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt
    13501 gggtgatcag ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg
    13561 ggtgatccgc tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca
    13621 gactctggcg cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac
    13681 caccaaccgg gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca
    13741 cgtcgaagtc gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct
    13801 ctttcgcgcc ataggcggca acgccctggg aacgaccttt cagaatggta ttttgcagcg
    13861 ccgctttctc gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcagac
    13921 gatcccagcc gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca
    13981 tacggaaacc cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga
    14041 agaacacggg cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc
    14101 tgttgatacg gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg
    14161 tgatcccggc cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga
    14221 ggtattcaat acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt
    14281 tctccgcacc acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt
    14341 cgagttgtaa aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga
    14401 ggaacatgaa gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg
    14461 tcagggcttc catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg
    14521 tagcgcgcgc cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga
    14581 tgcggcgcag gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct
    14641 cataccagtt agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg
    14701 ccagcgccac cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa
    14761 cggaaaaatc cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt
    14821 gtaagtcaaa cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca
    14881 gtaattgttc acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa
    14941 aggcatccgg cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc
    15001 gggtctgcca ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg
    15061 gtccgtggtg actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa
    15121 aaatcccttt gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg
    15181 caatgcgttc accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct
    15241 gatcgccaac cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca
    15301 tcagtgcctg catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg
    15361 ggatgaattt atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg
    15421 cacaggcacc cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa
    15481 taaccggtgc cattttggta aagcaggttc ctgcaaccac catcaggtca gcctgacgcg
    15541 gcgaagcacg caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag
    15601 tcaccatctc aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac
    15661 gaccccagtt aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct
    15721 ccagagggtc ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg
    15781 gatctatgcg ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag
    15841 cgatactgtt cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca
    15901 gcgcgccaat acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag
    15961 cttccacaaa gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca
    16021 gcgcttcaac gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc
    16081 gtaagcgggc ggagccgacc gagtcgatac cggattcaaa cggcacgttt ttcgacctcg
    16141 cgcgtgcgcg accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg
    16201 caacgataag aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca
    16261 tactcattgc ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac
    16321 gccacatcga ttcatgggga ggaataaaaa aaaccttaca ataactgtag aaattctttt
    16381 atacagctaa ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa
    16441 agcttcttca cattagttta cataatatca acaccattag catttaatgc cctttcaccc
    16501 cagatccttg acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa
    16561 tggaaaaaat acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga
    16621 gtattttctt gatctgtgac acgcttttgt tattccataa caaaaacgca gcaacaaatt
    16681 tacgtatttt ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcattc
    16741 gggacgtgaa tctctggtga ttgaaaaata aacagttttg tacgttctgc actatgcgag
    16801 gaaggatttt tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt
    16861 tacactattg tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca
    16921 acaatgaatc atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc
    16981 tctccatcgc ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca
    17041 gcaaatattc ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt
    17101 ccgggctcat catctcaacc ggccttgccg tcacgccaag accggctttc actgccgcac
    17161 gaacggccgg aagcgtcgag gcgacataag ccagtcgcca tggaatatct actttattaa
    17221 gcgtcgccag caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg
    17281 gctcgccttt ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca
    17341 ggttcaatgc cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg
    17401 attccagcat ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg
    17461 gataaaccga actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg
    17521 aagcaccgat agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat
    17581 cattaaaacg caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca
    17641 gtttgttgcg accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca
    17701 tttgctgact tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt
    17761 tcagatcggc aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac
    17821 gatttgcact tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg
    17881 tgcacacaca aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac
    17941 ccggacgaaa gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt
    18001 ataatgcctg aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact
    18061 cactcttttc tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc
    18121 cactctcgtg cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg
    18181 catcaagagc agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat
    18241 aagggtcatt gcgaattatc tgatgcaaag ttatgttatg ttaggcaaag taatcttcta
    18301 ttattaataa gcacatcaaa acctttttga atattaaata ataattaatt agcatcatcc
    18361 tcattcatta attccgctta acaatagttt cacaccttac ccccattgcg acacctcgga
    18421 aatcatcacg ctgttaaact ctccgtaatg cggaaatatc atcatcgcgt ttcattagtg
    18481 aattcttaaa tgaggcattt tcacacaatt atcttacaga taaaaaaacc agacttgcaa
    18541 ttaagaatta gaacaattca ctatataaca ttgcatgtaa agcatataca cctcattatt
    18601 ttgtcattat taagttatta acagcacaat cgagccttcc cctctggcaa aatcttattc
    18661 tgcagacctt caaaacaccg tcctggggga gtacattgtt ctaagctgac ttccacggca
    18721 gggagtggcg ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa aaatccagca
    18781 aattagagaa tgtctgttat gacatccgtg gtccggtgct gaaagaagca aaacgcctgg
    18841 aagaagaagg taacaaggta ctgaaactga acatcggcaa cccagcccca ttcggttttg
    18901 acgcgccaga tgaaatcctc gttgacgtga tacgcaacct gcctaccgct caagggtatt
    18961 gcgattccaa agggctttac tccgcgcgta aagccatcat gcagcactac caggctcgtg
    19021 gcatgcgtga tgttaccgtg gaagatattt acatcggcaa tggtgtatcg gagcttatcg
    19081 ttcaggcaat gcaggcattg ctgaacagcg gggacgaaat gttggttcct gcaccagatt
    19141 acccactctg gaccgcggcg gtttcgcttt ccagcggtaa agcggtgcat tatctttgcg
    19201 atgaatcctc tgactggttc ccggacctcg atgatattcg cgctaaaatt acgcctcgta
    19261 cgcgtgggat tgttattatc aacccaaata acccaaccgg cgcggtatat tccaaagagc
    19321 ttttaatgga gattgtggag attgcacgtc agcataatct cattatcttc gccgatgaaa
    19381 tttatgacaa aattctctac gacgatgctg agcatcattc aattgcgccg ctggcacctg
    19441 acctgctgac cattaccttt aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc
    19501 aggggtggat ggtgttgaac gggccgaaaa aacacgccaa aggctacatc gaaggtctgg
    19561 aaatgctggc ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg
    19621 cgctgggtgg ttatcagagc atcagtgaat ttattacccc tggcggtcgt ctttatgagc
    19681 agcgtaaccg cgcgtgggaa ctgatcaacg atattccggg cgtttcctgc gtgaaacctc
    19741 gtggtgcgct gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt catgacgatc
    19801 agaaaatggt gctggatttc ctgttgcagg aaaaagttct gttggtgcaa gggacggcat
    19861 tcaactggcc gtggccggat cacttccgca ttgtcacgct accgcgtgtc gatgatatcg
    19921 agctgtcttt gagcaagttc gcgcgtttcc tttctggtta tcatcagctg taatcttaat
    19981 ttcactgccg gagattgcat ccggcagtgt tatcccgcca caatgacctg atgatgtcat
    20041 catacgtaag gtcactatga aacagagcca tttttttgcc catctctccc gcctgaaact
    20101 cattaaccgc tggccgctaa tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt
    20161 gcaggtagcg atggtcgccc atgcgctggc agctatcaaa aatcggaaat ttggcggtaa
    20221 tgtcaacgcc gaacgtatcg ctttactggc gatgtaccac gatgcctcag aagtgctcac
    20281 cggcgatctc cctactccgg tgaaatactt caatgcgcaa atcgctcagg agtacaaggc
    20341 tattgaaaaa atcgctcagc agaaactggt cgatatggtt ccggaagaac tgcgggatat
    20401 ctttgcgccg ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc tggtgaaaca
    20461 ggcagatgca ctgtgtgcat atctgaaatg tctggaagaa ctcgcggccg gaaataatga
    20521 attcttgctg gcaaaaacgc gactggaagc gacgcttgaa gcgcgtcgca gccaggagat
    20581 ggactacttc atggaagtat ttgttcccag cttccatctt tcgctcgatg agattagcca
    20641 ggattcaccg ctgtaagcag ccggagtctg cgtcgcatca gtcaataagc gccggatgcg
    20701 acatcaggct cttgtcaaaa cggaaacagc atcgggatca tcaccacaca aaccgccatc
    20761 acgataatgg tgaacggtac ccccaacttc acaaagtcac tgaagctgta atttcccgga
    20821 cctaaaacca gggtgttaac aggtgaagac accggtgtca taaaggcggc ggatgctgcc
    20881 atcgccacga ccatcgcaaa tggataaggc gacactccca tcgttttggc agcagccagc
    20941 gcaatcggag ccatcaacac cgccgtcgcg gtattagaga taaacagccc aataaccgcc
    21001 gacaagacaa acaaacaccc cagcatcata tgtggcccat aaccgccgcc aatatccatc
    21061 agccctttca ccgccagcgc gacacctccc gttttctgta atgccacagc aaacggcatc
    21121 atcccaacga tcaaaataat gctcggccag tgaatggatt tataggcgct ttcagcatct
    21181 atacagcgga atttccccat cagcaggcag gcgatgatag cggcaacagg attaggaatt
    21241 tcatttgtca gcattaacgc caccattagc accagacaga aaatggcatg gggtgcctgg
    21301 ctgtgcgcgg gcgatgcttc actcacctct tccggtaagt tcagcgctac gaagtcgcgg
    21361 ccctgtttgg ccagcatacc gatcagtttc cagttaccca caaccaggat gatatcgccc
    21421 agcagcagag gctcatccgc cagcgaacct tccagcgcca cgccattgcg cttgagcccc
    21481 accacattca gtccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt accaatcagt
    21541 tctgactcag gaattaatga aatctctgcc atgcccacat caagggcctg gtcagaaaaa
    21601 tactcgccgc gcagtaccat cggctccagc aattgctcac tacaaaattg ccggagatcg
    21661 acatcagccg cagacatatc aataagcaaa acgtcacgcg cgcgaaattc agaaacccca
    21721 ttaacgttca cgataacgcg acgaaaacgc cgccagcgtt caacaccgat gacgttagcg
    21781 ccataacgct cacgtaattt gagatcatcc agccgttgac ctatcattgg cgatccgggg
    21841 cgaatagcca gacgtcgcgc acgcccggtc agtcgatatt cacggataag atcgcgaaag
    21901 gttcgacgcg tccagccttc gcgctgcggg gtctgggtat cccctttcag catgaaacgc
    21961 atcactaaca tatacaaaat acccagcacc agcacgacca ggccaatagg tgttacgcta
    22021 aagaaactga agccgtgata gccttcacgc agcaattcac tgttgactac caggttcggc
    22081 ggcgtcgcca ccagcgtcat catgccgcta atcagcccgg caaaacttaa cggcatcatc
    22141 agacgtgacg gcgaagtttg catacgcatg gcaacgctta acaccacggg aataaagata
    22201 gcgacaacgc tggttgaact cataaacgcg ccaagccccg cgacggtcag catcaacaaa
    22261 accaacattt tgatttcact gttgcccgcc actttgacca gccatgttcc cattacggtg
    22321 gcaacaccgg tacggaccaa accatcgcca ataataaaca aggcggcaat caggacaacg
    22381 ttaggatcag aaaagccgga aaatacttcg gggaccgtca gcgttccgct taatgcaaac
    22441 gcgacaataa caaacaaagc gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg
    22501 atggcaacgg ccagtaatga aagaacccaa atcaattcac cgttcacaac ttatccttgt
    22561 taattgaggg ggatgacttg attctgccat aaaaaagccc cgacgagacg gggctaaatc
    22621 atgatcaggt gtttcactga ataataacat cgccatttgg ctgtttggtc acagtaattt
    22681 gctccagact gtggaggacc aaatcgacct cattcaggcg cggggtatct gccggagcgt
    22741 taaccgcaat gacatggcaa cccgccgcca ggccagaaag cacgccagcg ggagcatctt
    22801 ccaccaccac acactcctgc ggcgcaagcc ccagcagctg cgcacctaac agatacgcat
    22861 caggttctgg ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa
    22921 gccccgctat tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc
    22981 acggaatacc tgctttattc aaatgattga gtaaggcgat tgccccagga agcgcggtaa
    23041 taccttcggt ttccgtggcc tcgatttgct ccagacgcgt aaactcggcg gcaatatcag
    23101 cctcggattt gcccgccata aaatggcgca aagaggtgat cgcctgttta ccgtgaatga
    23161 aagccagcac ctcttccggc gctaacccat gacgtctggc ccagttgctc cacgcccgtt
    23221 ctaccgcagg cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc
    23281 gcacgcgggc ctcctcaggc gttgataatt tgattaattt cgttggcgct caaatggtac
    23341 tgacgcgggc aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc
    23401 tgggcgttaa aaccatgagt cccagcgtca aaatgggtat agcgcccttc cacattaacc
    23461 ataaagcgga cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg
    23521 acacgacgtt catcgatgga ttgctggtcc tgcaaattag accaggaaac atacaacgca
    23581 tgatacatct ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac
    23641 tcgcgatcca attcacgcat ctgtaatccg taaccacgct caataattgt ttgcaggcga
    23701 cggtaacgtt cagcatttgc cggatcgagc atagtcatca tcttgtactg attagacaaa
    23761 ataagacgtt gcgcgttggt catttccatt gttgactcct gtatcactct actacggtga
    23821 aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc
    23881 atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg
    23941 gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg
    24001 aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac gcttgcccag
    24061 ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc
    24121 aagcagaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa
    24181 acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg
    24241 tttttccagt ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt
    24301 aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa
    24361 aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc
    24421 actatcaggt atcctttagc agcctgaagg cctaagtagt acatattcat tgagtcgtca
    24481 aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat
    24541 ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca
    24601 tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac
    24661 ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc
    24721 catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct
    24781 gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc
    24841 aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc
    24901 agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta
    24961 taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt
    25021 tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc
    25081 acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga
    25141 agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta
    25201 cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa
    25261 accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc
    25321 tatccgcaac ggtaaatgcg ttgacacctc tatgggtctg accccgctgg aaggtctggt
    25381 catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct
    25441 gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct
    25501 gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc
    25561 gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc
    25621 gctgatggat ggtcgtctgg acgctgttgc attcactggt ggtatcggtg aaaatgccgc
    25681 gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga
    25741 acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc
    25801 tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac
    25861 tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc
    25921 ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc
    25981 gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt
    26041 ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatacgcc cgatcagact
    26101 acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc
    26161 tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca
    26221 aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca
    26281 cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa
    26341 atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa
    26401 ctgacccgca acagcttcgg tggtgccaaa aacaccaaca tcaccggcgt tatcgttaac
    26461 aaactgaacg caccggttga tgaacagggt cgtactcgcc cggatctgtc cgagattttc
    26521 gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc
    26581 ccgctgccgg ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc
    26641 gatatggctc gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc
    26701 gttaaatccg tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc
    26761 ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca
    26821 gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg
    26881 cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac
    26941 accaacacct ggcagacctc tctgagcctg cagagcttca acctggaagt tccggttgac
    27001 gatcacgaac gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg
    27061 atcgaatctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt
    27121 tatcagctga ctgaactggc gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac
    27181 gaaccgcgta ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta
    27241 ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactaggt
    27301 gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc
    27361 gagctgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac
    27421 gtggtgctcg gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct
    27481 gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc
    27541 agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt
    27601 gactgtgcga tcaacccgga tccgaccgct gaacagctgg cagaaatcgc gattcagtcc
    27661 gctgattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc
    27721 ggtacttctg gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag
    27781 gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct
    27841 gacgttgcga aatccaaagc gccgaactct ccggttgcag gtcgcgctac cgtgttcatc
    27901 ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgccgacctg
    27961 atctccatcg ggccgatgct gcagggtatg cgcaaaccgg ttaacgacct gtcccgtggc
    28021 gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag
    28081 cagtaatctc gtcatcatcc acagctttgc gctgcggata tctgaaccgg aaataatcac
    28141 tatttccggt tttttattct cttaatttgc attaatcctt tctgattatc ttgcttaact
    28201 gcgctgcatc aatgaattgc gccatctcac tttgcatact taccactttg ttttgtgcaa
    28261 gggaatattt gcgctatgtc cgcaatcact gaatccaaac caacaagaag atgggcaatg
    28321 cccgatacgt tggtgattat cttttttgtt gctattttaa ccagtcttgc cacctgggta
    28381 gttccggtgg ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa
    28441 acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct
    28501 gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc
    28561 ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat catcatgttt
    28621 atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt
    28681 atcctggcgc ttattcgcca tacccgcggg aatgaaattc tctttattcc tgcgctgttt
    28741 attctgtttt cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc
    28801 attatcatcg caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg
    28861 acttatattg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt
    28921 gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg
    28981 tgggttatcg ccactctgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa
    29041 aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg
    29101 gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt cctgacagcc
    29161 gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc
    29221 agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac
    29281 ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc
    29341 cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggttggtaa tggtgaagca
    29401 ggtaatgcca gcgtgttaaa tactatcctc aacagcattg ccaatgccat tagcggtctg
    29461 gacaacgcgg tcgcggcctg gtttatgttg ctcttccagg ctgtatttaa tttcttcgtg
    29521 acgtccggtt ctggtcaggc ggcattaact atgccgttac tggcaccgct tggcgatctg
    29581 gtcggtgtta accgtcaggt taccgtgctg gcgttccagt ttggtgatgg cttcagccac
    29641 atcatttacc caacctcagc ttcgttaatg gcaacgctcg gtgtgtgccg ggtggacttc
    29701 cgtaactggc tgaaggtggg cgcgaccctg cttggactgc tgtttattat gtccagcgtc
    29761 gtagtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg
    29821 ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc
    29881 cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct
    29941 tccggcgtca gccagcaaac tccactgact tcatcttcct gtagggcgaa gggaccgtga
    30001 gagacgcaac tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc
    30061 ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg
    30121 gattccagca gttgctcatc ggcctgaact acaccgcctg cggtcgcatc taacatgccg
    30181 ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc
    30241 acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct
    30301 tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga
    30361 cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg
    30421 tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt
    30481 acactaagta cgtcattatc cagcatgcca taacccgccg gattaccgcc tttcggaata
    30541 ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg
    30601 gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtagattttc cgggccaaaa
    30661 agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg
    30721 gttatcggga aatgcagcat catttgatcc acttcgctgt cgcagttgcc acgcacagcg
    30781 ataaccttat gtgccacttc attaagccgt tcagcgactt tggctggcgc gtaaccctcc
    30841 ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca
    30901 ccgctttgga caaacaactc cacaacacgt tccgtcgccg gtaacgaccc atgaatgtcc
    30961 gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg
    31021 attctgtcgt tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga
    31081 cgctcgctgc cactggaatg tcgcataatc caccaggcgt tctggcacct catcgccatg
    31141 tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa
    31201 taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact
    31261 ggcttttccc tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg
    31321 acgctcttcg cggatgggca tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg
    31381 cgcacgattt tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc
    31441 ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa
    31501 cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc
    31561 gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga
    31621 taacacataa ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg gtttactcat
    31681 tatgtcctcg gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca
    31741 cgtgacgaat actcacgtaa gtcgccgcgc tttctatact tgtggtgttc agatgattaa
    31801 acaaccggag ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt
    31861 acgctgtttc tcgaagaagc agagcttgat tatcgcttga ttaaggtaga cctggggaaa
    31921 ggcggtcagt ttcgtccgga atttttgcgc atttcgccca acaacaaaat tccggcaatt
    31981 gttgatcatt ctcctcccga tggcggcgaa ccgttaagcc tctttgagtc tggtgccatt
    32041 ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc
    32101 gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat
    32161 catcatttta atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt
    32221 gaaactcagc gtctttacca tgtactaaac aagcggctgg aaaactcgcc ctggctggga
    32281 ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc
    32341 ctgcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg
    32401 cgccctgcca ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg ttcggatagt
    32461 taacagaaac aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa
    32521 tggcacaacc tgccgctatt attcgtataa agaaccttcg tttgcgtacg tttatcggaa
    32581 ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc
    32641 ccgccgataa agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa
    32701 cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa aaattaactc
    32761 aggatgtgct cgatatcgca cgtgaacatc actgggtgac gtatgctgaa gtggagatcg
    32821 ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt
    32881 aatcgccata tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg
    32941 tcgccatttg attccgcgtt tgctggagct gggccatcaa attacggtag tgacgcgtaa
    33001 cccgcagaaa gccagttccg ttctcggccc tcgggtgaca ctatggcaag ggcttgccga
    33061 tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga
    33121 taaacgctgg actcacaagc aaaaagagcg tctctgccaa agccgctgga atatcacgca
    33181 aaaactggtc gatttgatta atgctagcga cacgccgccg tcggtactca tttccggctc
    33241 ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca
    33301 taacgaattt actcataaac tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag
    33361 tgacaaaacg cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg acggcggtat
    33421 tctcggtaaa atgctgccac cgtttcgtct tggcctgggc gggccgattg gttccggtcg
    33481 gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga
    33541 taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt
    33601 tgcccatgcg ctcggtcatg cgctgcatcg cccggctatt ttgcgcgtcc ccacaaccgc
    33661 cattcggctg ttaatgggcg aatcttcagt actggtatta ggtggacaac gcgcgctgcc
    33721 taaacggctg gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc
    33781 ggatgtcgtt cgctgatgtg gtttacagca aacatccgcc agttaactcc ctgtgttaca
    33841 ggattagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa
    33901 ctctctgtct aaacctctat ccagcatctc ctgagcaata cgcagggctt cttcgtgttt
    33961 gccctgcatt gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt tctccttttc
    34021 ttgtggtgcg cgttctgtta tctcaccaat aaatgcacgg aaacgctggg catcccctgt
    34081 ttgtaatacg taattaaaca gggcttttag ctgtctgtca ttagtgttcc ctgtaactag
    34141 cagcgaaaca atttggtcga ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat
    34201 taactccaac agcgccattt tgcggtgttg cataatctcg tcatccggca ccacggtaat
    34261 atccaccaac ggaaacgctg atgaatatat tttgcgggct atagcaggct cggcaaattc
    34321 atcaagccag cagagtgaat aaggataagg acttctgcaa ccatgataaa acagcatcgg
    34381 gatcaccaat ggaagctctt tatagcccgc atcaagatgg ttttgcattg ccgcaatgga
    34441 ataacgcatc atgcgaaaag ccattaattc ttccggctta ctttggtgct ctatcactac
    34501 ataaatataa cccactccct cctgcgtttt cacagaccac aagaggtcgg aataatattg
    34561 ccgcaggtct tcatcaataa aactgtttgg ttctagttta agcgtcgtta aatcacacag
    34621 tttgcgcagc ggcgcgggaa gatgaatatt aataaaattc cgcgcggtgt ctggatggcg
    34681 taaaaaagat ttaaataccg catcatgcgg cgtggaagtt gtcgatattg tcatggcgtt
    34741 ccgtcacccc tcaaatttaa gatgacgcga caataaccac gattaacacc caatgcagca
    34801 ggcagttttt atctttggac agcgaattca gaagtatttt tagcggactg caacttttac
    34861 atttcagcgg aagctgactc gcaaattccc cttgaaaata ctccggcagc gttagccacc
    34921 ggagaggaaa ttatttcagc gatcccttaa ggaactgttg cagacgaggg ctttgcgggt
    34981 tgccaaataa ctgctccggc gcgccctctt cttctatttt cccctgatgt aggaaaatga
    35041 cgtgagtaga aacatggcga gcaaagccca tttcgtgagt cactaccacc atggttttcc
    35101 cctcttctgc cagttgctgc ataatacgta acacttcgcc taccagttca ggatcgagcg
    35161 ccgaggtagg ttcatcaaac agtaaaactt ctggttccat cgccagcgcc cgcgcgatag
    35221 aaacacgctg ttgctgaccg ccggaaagat gcaccggata tttcccctgc gcacgttcgt
    35281 ctatcccgac tttcgccaga tacttcaccg cccgctcgcg cgcttcctgc ttgctcaggc
    35341 ccaacacctg aatcggcgct tccatgacgt tttccaacac cgtcatatgg ctccagagat
    35401 tgaagtgctg gaataccatc gtcaggcatg tgcgcagtaa gcgcagttga tttttatcgg
    35461 cgactttgag ttgaccgtct ttgtcgcgca ccagattgat cgtctggcca ttgaccacga
    35521 tcgatccttc actcggtttt tcgaggaagt taatgcagcg cagaaaggta cttttccccg
    35581 atcccgacga tccgatgatg cttattacat ctccggcatt cgcttgcagt gagacccctt
    35641 tcagcacttc gtgttcgcca tagcgtttgt gcaaatcgat aacgtttaat ttattctcgg
    35701 acatcgtatt ctcagtgcgt tgaagaaggt ttcacatgct gcaaccagcg tttttccgct
    35761 ctgcgaaaga ggctgatcag gacataagag atgattaaat agagcaccgc ggcaatgccg
    35821 aaggcggtaa aaggttgata cgtggcggcg ttaatatcgc gggctatttt cagcagatcc
    35881 ggcacagtgg ccgtaaacgc cagcacagta gagtgcagca tcaggatcac ttcgttgctg
    35941 tatgccggta acgcaatacg cagcgcagaa ggcaaaataa tgcagcgata cattttaaag
    36001 gtcgagaagc cataggctct ggcggcttca atttccccat gcggaaccga acggattgcc
    36061 ccagcaaaaa tctcggtagt gtaagcgcag gtgttaagcg tcagcgccag cacggtacag
    36121 ttcaggccac tgcggaagaa agcgttaagg aattcggttc ccttaacaat ctcaagcgtg
    36181 tacatgccgg aatagaacac cagcaactga acatacagcg gcgtaccgcg aaaaatatag
    36241 gtaaataacc agattggaaa ctggatgtat ttattactgg agacacgacc aatcgccaga
    36301 aacagcgcca ggactccgcc tatcactacc gacaaaataa gcagccacag agtgatcgcc
    36361 acaccagtaa agcgataacc gtcggtccac agcagcggtt tccagtattc atgtaagatt
    36421 tcgatcacag gtcagccctc ttcacaccca cggagtagcg gcgctcaagg aacagcagca
    36481 caccattgga aacggtggtg aaaaccaggt aaatcacgcc acagacgatg gcgaaataga
    36541 acggttccca ggtacttttg cctgccagct gcgtggcttt gaccacatct tccaggccga
    36601 gtaacgaaac cagtgcggta gatttgagga tcacctgcca gttgttgcca atgcctggca
    36661 gggcgtaacg catcatcgcc ggaaacatga tccgccgaaa cacttgcccg cgagtaaaac
    36721 cgaacgccgt cgccgcctct atatgccctt tcggcactgc cataaacgcg ccacggaacg
    36781 tttcggtaaa ataagcaccg tagataaaac cgagagtgat aataccagcg accatcggat
    36841 cgatatcaat ctgcccgacg cccatcgcct ccgtcaccat gtttagcgca atctgcaaac
    36901 catagaaaat cagcagcatc aacaccaagt ccggcacgcc tcgaatcagc gtggtgtacc
    36961 cttcgaaaat cagccccgaa agccgatttt gcgagagctt accgccagcg ccaattaaac
    37021 cgatgattac agcgagcact acagagctga tagccagctc cagcgtgacg agcgcaccct
    37081 gtaaaataac acctgaaaac ccatacaaca tgctgcctgt cctgtcgtgt gtggtgaatt
    37141 accgcctctt gtctccctcc ggttacccga agggagcggg ctttctttca ccgatggcga
    37201 ttaaccacca taaacatcaa aatcgaagta ctttttcgct aatttctcgt aagtaccgtc
    37261 agcgcgcatt tcggcaaagg ctttgttcag tgcctcgcgc agttcgttat cttctttacg
    37321 caggcccatg ccggtcccta cgccaaacag tttttcatct ttaacagacg gaccaccgaa
    37381 tttgtaatct ttaccgacgg gttgtttgag gaaaccttcg ctggcagcga cctcatcctg
    37441 gaacgcggca tcaatacgtc cggcagtcag gtcagaataa atgttgtcct gcccctgata
    37501 cgagacgatt tcaatgcctt ttggtgccca gtgttcatta ccgaacgtct cctgggtggt
    37561 gccctgcaat acgcctaccc gtttgccttt cagcgactcg actgtcggct gaatgtcaga
    37621 atttttcgcc accaccagac gagaatcggc agcgtacagt ttgtcggtga aggcaatttc
    37681 ttgctgacgt ttttccgtaa tggaaagcga tgacatgatg gcgtcaatct tcttcgcttt
    37741 taaggacggg attaacgcat ccagcgggtt ttcgacaaac gtacattgcg tattgatgcg
    37801 cttgcacaac tccttcgcca gctcgatatc gaaaccaacc agttcgcctt gtgaattttt
    37861 tgattcaaat ggcgcatagg tcgggtcggt accgatgcgg atgttttgcg gaattgcagc
    37921 aagtgccgca gttgcgctgg agaaggccag aaccagagag agcgatagca ccagtttttt
    37981 cataatgtcc tcaactgaca gtctttttat agggaatatt tacaggtttg ttacacttat
    38041 cgtgccataa aattgaccat caaggcaaaa tatcccgccc cgttagacgt attatttttc
    38101 attaaaaatg cttaagtatg catttcaatg caccacgata gtgcaacaaa cctggcgtgc
    38161 accatgatga tcattccatc aggtacagct tcccagcgac gtatcagtca ccgtagacat
    38221 taaagtcgaa atactttttc gccatcttgt cgtaggtgcc gtcctgacgc agctcgccaa
    38281 gcgccttatt gaaggcagcc gtcagttcag catcatcttt acgtagccct acaccggtgc
    38341 catcaccgaa gtattttttg tcttttactg atgagccagc aaaagcgaaa tctttaccag
    38401 caggttgctt gaggaatcct tcgctggcag caacttcatc ttgtaacgca gcatccagac
    38461 gtcctgcagc cagatcggaa tagaccaaat cctggttggc ataggccacc acatccacgc
    38521 ctttactacg ccaggtctcg ttagcgtaag cttcctgggt tgatccctgc agcacaccaa
    38581 catgtttacc tttcagtgaa tccagcgttg gctgaatcgg tgaacctttg gcagcaatca
    38641 aacgagaatc tgcggcgtac agcttgtcgg agaaggcaat ctcctgctga cgtttatcgg
    38701 taatggaaag cgacgaaata atagcgtcga tttttttcgc tttcagtgag gggatcagcg
    38761 cgtcaaagtc actggcaacc taggtacatt tcacctgcat ccgtttgcac atctcgttac
    38821 cgagatcgat atcaaagcca acaaaatcac ctttagcatc tttcgacgag aacagcgcat
    38881 aggtggtatc ggttccgata cgtaccgtct ccggtagcgc cgcatagctg gaagccgctg
    38941 cggagagacc gactaataaa gacagagcga gaatcgactt cttcatacat aaccctcaag
    39001 tgaaaatggc ttttttatgt tgtgtattgt gttgtgtgtt tgcaggctct ttcatgcagg
    39061 tcttatgcca tcttgacgac aacagtaaca ttcaacgtta aatatgttaa taagacgttg
    39121 cattattgtc ctgaagttga agatagcagg tatagcggtt gaatcgcagc gtttcgattg
    39181 aatggcagaa acaaaatgtc gaggatttga tcgcggttgc aaaattgccc tgaaacaggg
    39241 caacagcgga gttatgcgcc ctgccagcgg gcaaagagat cttcaggaag ggttattgca
    39301 aactggtcaa gaacacgatt aaccgtctga tttatcacat catcaaggga ttgcgggcga
    39361 tgataaaacg ccggaacggg aggcataatc accgcaccga tttctgccgc ctgagtcatt
    39421 aaacgcagat ggcctaagtg caatggtgtt tcacgcacgc agagcaccaa cgggcgacgc
    39481 tctttcagca ccacatctgc cgcacgggtc agtaagccat cagtatagct atggacaatg
    39541 ccggacaggg ttttgattga acagggtaaa atcaccatcc ccagcgtctg gaaagaaccg
    39601 gaagagatgc tggcggaaat atcgcgcgca tcgagcgtga catcagctaa tgcctgcact
    39661 tcgcgcaggg aaaaatccgt ttcgagggat aaggtctggc gcgccgcctg gctcatcacc
    39721 agatgcgttt cgatatctgt gacatcgcgc agaacctgta ataagcgcac gccataaatc
    39781 gcgccgctgg caccgctgat gcctacaatg agtcgtttca taaatagttg ccctgtcaga
    39841 cttgcaggca gactttgcag tatttcggcg ggagttgcaa gtcagggtgc cagaccggca
    39901 ccctcagcga aggcatcatc cttcgttatg catttcgaga ttttccactt cgttctgacg
    39961 ttgcactgct ttggcgtcat cattacgtaa cgtatcgagg aaatcgaggt agccctgatc
    40021 aacatctttg gtgacgtaga cgccgttgaa caccgagcat tcaaactgct ggatatccgg
    40081 attttcagcg cgaacggcgt cgatcagatc gttcagatcc tggaaaatca acccgtcagc
    40141 accgatgatc tggcgaattt catcaacttc gcgaccgtga gcgatcagtt ccgtggcgct
    40201 cggcatatca ataccataaa cgttcgggaa gcgaatttcc ggtgccgcag aagcgaggta
    40261 cactttcttc gctccggctt cgcgtgccat ctcgataatc tgctcagaag tggtgccacg
    40321 gacgatggag tcgtcgacca gcaggacgtt tttatcgcgg aactcggcgc ggttggcatt
    40381 cagtttacgg cgcacggact tacgacgcag ctgctggccc ggcatgataa aggtgcggcc
    40441 aacatagcgg tttttaacga agccctggcg gtacggtttg cccagaatac gagcaatttc
    40501 cagcgcgata tcacacgagg tttctgggat cgggatcacc acgtcgatat ccagatcttc
    40561 ccattcgcgg gcaattttct cgcccagttt cgtgcccata ttcacacgcg cgctgtaaac
    40621 ggaaattttg tcgataaacg agtccgggcg ggcaaagtat acatactcaa acaggcacgg
    40681 attgctgacc ggattgtcag cacattgacg ggtaaacaac tgcccttctt cagtgatgta
    40741 aatcgcttcg cccggcgcga cgtcacgcag gaaatcaaag cccagcgtat cgagcgctac
    40801 gctttcggaa gcgaccatat attctgtacg gttctcgtca atatcacgtt ttcccagtac
    40861 cagcggacga atcccgtttg gatcgcggaa agcaaccata ccgtggccga taatcatcgc
    40921 cacacaggca tacgcgccgc ggattaagcg gtttgtggca gcaatggcag cgaaaatatt
    40981 gtcggcttcc agcgggtagt ggcggaagtt gtccagctcg ctggcgaaga tattaagcag
    41041 aatttccgag tcggaagtgg tgttgatgtg gcggcgtttt tcttcaaaca gttttttacg
    41101 caactcgtga gcgttggtca gattgccgtt gtgggcaagc gtaatgccat acggggagtt
    41161 aacgtaaaac ggctgcgctt cagaggcgct ggagctgcca gccgtagggt aacgcacatg
    41221 accaatgccc atattgccct gcaaacgctg catatggcga gcttcaaata catcgctcac
    41281 cagcccgttc gctttacgca aacggaagca gttattggca tctatggtga tgatgccggc
    41341 ggcatcctga ccgcgatgct gaagcaccgt taaggcatca taaatcgact ggttaaccgg
    41401 cataacaccg gcgataccga caataccgca catacgtctt tttcctcgtt aagccacatc
    41461 tcagagcact tacgctctag gcaagaaact tgacgagctt tgcagataat caaaaaacca
    41521 tctgatgata aaactgaatt gcgggatcag ctgtgatttg ctccagtctt cacttttcga
    41581 caccccggta aaggagtcga gaaagaagag aatggcggca acaatcaaca caccgcgcaa
    41641 cgcaccgaaa cagacgccca gcacccgatc ggtgcctgac aaccccgttt tctccaccaa
    41701 ctggcctatc acgaagttca cgatagcacc aacgatcagg gtagcgataa acagtaccgc
    41761 gatggcaatc ccatttcgaa ccagttcgtc ttcaaagccc gtaaaccaga ctgacaggta
    41821 agtgtagtaa tgactggcaa caaagaaagc acaaccccat gtcaccagcg ataacgcttc
    41881 acgaacaaag ccgtggatca ggctaaccag agaggaaaaa gcaatcaccg cgattatggc
    41941 gtaatcaatc cagaccatat gtgtcccacg attttacgcc ctgtcatcct gttcggggcg
    42001 cattctaaca gaaaaagaaa acgtttgcgt agggatttcc ttcccgcgca tcaataaaaa
    42061 tggcgctgaa aaaatattca acgccatcga ctttttatgc ctttgcggca tcgtgcaatg
    42121 cgtgtcggat gcggcgtaaa cgccttatcc gacctacggt tctaacactg cccaagcctg
    42181 ataagacgcg ccagcgtcgc atcaggcaag actgtattaa ttcggcgtat agcccattac
    42241 cacgccactt aagccagaaa gttgcttcaa ctcacccagc gaacctttca gcttatcttt
    42301 cgaggcatcc ggcccaacca gaatacgggt aattttaccc tgcactggcg tggatggcga
    42361 cgtataaacc cgataaccgg caccgcgcag cttaccgaca atctcattca ctttatcggc
    42421 atttttcagc gcacccagtt gcacaacata ggctttaccc gttggcgctg ctttttcttc
    42481 cacgaccggc ttcggttctg gcttcggcgc aggtggtgct tcaacctttg gtttaggcgg
    42541 ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa actcggtgtt
    42601 attggctgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg cccgcacctc
    42661 ttccgctgcg ccttccggcg gctgcgtcgg caacgcctgg gtggcggctg gcatcatatc
    42721 aggctcatca cgatcgcccg ctttcggcac cagcgggata gccgcgaact catcctgata
    42781 atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg ccaccagcac
    42841 gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc gcgtcaatca
    42901 cttccatgac atgtgcgacc gtgtggaaag aaccacacac cagcacggtg tcttccgctt
    42961 tagcgtccgc cattgcggca tcccatgcct gcgcaacgct atcaaatgat ttgccgttac
    43021 ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat
    43081 accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct ttatcatgta
    43141 gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata cgcccggtga
    43201 gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc gactcgctca
    43261 caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac
    43321 tgacttccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga
    43381 caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat
    43441 cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcacct ttttcctgcg
    43501 ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt ttttcgctgc
    43561 ggaagatgcc tgctttctcg cgaccaatac tttcgcgatc tggacccagc cagtcggtat
    43621 gatccagcgc aatactggtt actaccgcga catcggcatc gacaatattg gttgcgtcca
    43681 gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgc ttgaacagcc
    43741 acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg cgtgccgatt
    43801 caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcctggccc tgcacacgta
    43861 cgcgctcggt ataacgcacc agatgaggcg aactgtagac gcccactttg taccctgccg
    43921 ccatcagaat cgactccagc gtacggcagg tggtgccttt gccattcgta cccgcaacgg
    43981 taaacacaaa tggcgctggt ttcaggacgc caagacgcgc cgcgacctgg ctcacgcgct
    44041 caaggccgag atcgatagtt ttactgtgca ggttttccag ataagaaagc cacgaagcca
    44101 gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat tcgttacggt
    44161 gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag gcctcaggtt
    44221 cctgatccgg taccgggggt accactacgc cttcacgcgg cgcttcagga ttcggcgctg
    44281 gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc ggacgacgga
    44341 cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat ctaggcggca
    44401 gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt aacgctttag
    44461 gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg cccatcgtcg
    44521 ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc agtgccgcag
    44581 aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg ccaccagagg
    44641 cggagaagca gatcagcggg cagttatctt cctgcgcctg ctcaacggca cgcacgaaac
    44701 gtgcacccac aacagacccc attgaaccgc ccataaaggc gaactcgaat gccgcagcga
    44761 caaccggcat tccatacaga gtgcctttca tcaccaccag cgcatctttt tcgccggttt
    44821 ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac ttcagctcat
    44881 ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac aggctatgca
    44941 ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc tcaagattac
    45001 gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc cacacccctt
    45061 caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca atccagctca
    45121 ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg gacgcataat
    45181 gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca gcccgaaact
    45241 ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc gtgccgcagc
    45301 gcgtttgtga cggattattt cgatgacgcc cggcaaaata gaaaccacaa taatcccgac
    45361 gatcagcagc ttaaggttat cctgaaccat cgggattgta ccgaagaaat agcctgcgta
    45421 ggtaaaaagc agtacccaca acagtgcgcc gatcacgtta taagcagcga aatgacggta
    45481 cgacatgtgg cccattcccg caacaaacgg ggcgaacgtt ctgacgatcg gcacaaaacg
    45541 ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgggttt tgtcgagata
    45601 actgcgacgg aaaattttcg aattgggatt actgaataac ttttcaccga acaaccgccc
    45661 gatagtgtaa ttgaccgcgt cacccacaat cgcggcgatc agcatcagca ctaccatcat
    45721 atggacgttg agatcgttgg tttccagtga tgccaacgct ccggcaacaa acagcaatga
    45781 atcacccggt aaaaacggca ttaccaccag accggtttca cagaacaaaa ttaagaacaa
    45841 aatggcataa acccagacgc cgtactccgc gactagctct gccagatgca cgtcaatgtg
    45901 caggataaaa tcgatgagga aataaatcag gtccatattt gcctatgcct tgtactcgtc
    45961 atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat tattctcgtt
    46021 agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg tcagggtaat
    46081 ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc gttctgtcct
    46141 ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg acttccatca
    46201 ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg atatcaacca
    46261 ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac ggggttcggg
    46321 actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag cattgcgcag
    46381 cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct ttactcagta
    46441 ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc gtggcgctaa
    46501 atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg tcaccaggta
    46561 aattcgcatt tacgcccaag gtccacgccg cgtctttgcg ctgcgcggtt gtttcgaaat
    46621 gcaccacttg cccggtaccg tgtacccctg cgtcagtacg cccggcgcag aagacggtga
    46681 tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca ctgcggactt
    46741 cattctgccg ttgccagccg taatacttac tgccgtcgta ctcaatgccc agcgcaattt
    46801 tataaactgg cagttgttgc tggtcggaca ttagtacaga tactcctgca ccagtttctc
    46861 ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagaa
    46921 ctggacttgc tccggcatac cgtagtcatt acgcacgcag ccaacagaaa gatgcggcgt
    46981 acccgaagca tcccctacct gagtcgggaa ttcgttctct tcagagagca caatatcttc
    47041 gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt
    47101 gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg aaatcatcag
    47161 cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt cacgcacgct
    47221 accttcgcta tccggcaaca acggcagcat attaaacgcc agttgacgac cgaagaaatc
    47281 ttcttcgtca atcggaatgc cgttgagcaa tttcgcactc tgccccgcta acgcatcgac
    47341 cgcttttttg ccctgggcgg aggctgaaat caggctggta acactgatac gcgacaaacc
    47401 gccctgatcg attaacggtt tcagtgccgc cagcagctgg ctggtcagac tgtctggtac
    47461 ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt ccggcaccac
    47521 cagcggtacg tcgggttcga gagcaaacag tccgctgctg tcgatcacca ggcaacctga
    47581 gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa aaaatgccag
    47641 ctgcgcctgc gtccagtcga attcagcggc atcctgcacg gtgattgttt taccaccaaa
    47701 gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc caaccgggaa
    47761 ctgacgttca gccagcgttt caagcagggc ttcgcccata gcgccagttg cgcccaggac
    47821 ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag ctccctgcca
    47881 atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac ggcgttaaaa
    47941 cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata cagagatgac
    48001 cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg tatcccggcg
    48061 actttacgca gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac cagccttttc
    48121 agcgtcggtt gatcgagcgg accatgcagc gtaatgcggc caaactcagg cgcgggcagc
    48181 aacgtatcca gcgcaacgtg ttgttcatgc ccaataaact tgctataggc ttcaaacact
    48241 tgcgtcgtgc cacgcgcttt accctccagg gtatagcctg cgatatgcgg cgtgccgata
    48301 tccacttttg tcagcaattc cacattaagt tccggctcgc cttcccagac atccagcacc
    48361 acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc gacgactgcg
    48421 ccacggcagg cgttaatcag aatcgctccg ggcttcaggc tacggatcag tttttcatcc
    48481 gccagatgta gcgttttgta cggaccatct ttaaagagtg gcgtatggaa agtcagaata
    48541 tccgcgcgct ggactaactc atccagcgag cggaaatcac cctcatcccc acggtcggcg
    48601 cgaggcggat cgcaaagtaa ggttttaatc cctaacgctt ccagtcgcgc ctgcaatcga
    48661 cgtccaacgt taccaacgcc cacgatcccc accgtacggt cgtgcagtga aaatccatcg
    48721 cgttcggcaa gcatcagcag ggaggagaaa acatattcca ccaccgcaat cgcattacag
    48781 ccaggtgcag cggaaaaacc aattcccgcc tgcttcaacc atgcttcatc gacatggtcg
    48841 gtccccgcag tggcagttcc aacaaattta atgggttttc ctgccagcaa agattcattc
    48901 actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc gacggggatt
    48961 ggacgcccgg gaaccgcgat cacctcaccc aaacggctaa ataagtcgcg ggcataaggc
    49021 atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga gagttaaccg
    49081 gacaagtatg ccataatctc gcggccaggc atacttgcga agatttcagg tataaggata
    49141 cgtaatgata caacctattt ccggccctcc tcctgggcag ccaccaggtc agggagataa
    49201 cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt cgctggaaag
    49261 cttaatgacg aaagtgacct cactgacgca acaacaaaga gcagaactgt gggcgggtat
    49321 caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact tccctgccgc
    49381 tgagcataat ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg cccgccagct
    49441 tttagcgcaa ttaggggagt atttacgtct ggggaatcat cgtcaggcgg tcacggatta
    49501 tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc aattaaaaac
    49561 cattctcacc ctgttgcagg aagggaagat ggttattccg caaccacagc agcgcgaggc
    49621 gaccgaccgt cctttattac cggcggagca caatgcgcta aaacagctgg tgaccaaact
    49681 tgcggcggca acgggggaac ccagcaaaca gatctggcaa tcgatgctgg aactttccgg
    49741 ggtgaaagat ggcgagttaa ttccagcgaa actgtttaac catctggtga cctggctaca
    49801 ggcgcgtcag acgctaagcc agcaaaatac gccgacgcta gaatcactac agatggcgct
    49861 aaaacaacct ttagatgcca gtgaactggc ggcgttatcg gcatatatcc agcaaaaata
    49921 tggcctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc ttaatcagct
    49981 ttatcaacgg cgggttaaag ggattgatcc gcgtgatatg caaccgctgc ttaatccttt
    50041 tccaccgatg atggacacgt tgcaaaatat ggcaacgcgt cccgcgctgt ggatactgtt
    50101 agtcgcgatt atcctgatgc tggtctggct ggttcgttaa ccccgacgaa atgacagtat
    50161 cgtgacaata atacccagca ccgcagagat cgccccggca agaaataccg aagagtaacc
    50221 aaacgtggtc gccagcattc ccgccagcgg cccggagacg ccgagggcga tatcctgaaa
    50281 cgcggcgtaa ccgcccagtg cggtgccgcg aacttgtgag gggacgcgtt taaccacctc
    50341 cacgcccagc gcaggaaaga taagcgaaca tccggctccg gttaacgccg cgcccgctaa
    50401 tgcgacccat gcacctgggg cttgccagag cagcaacaag cccaccgttt ctacaagcag
    50461 agagacaatc gccactttca cgccgccaaa acggtccggc atccagccaa acatgacgcg
    50521 catcacgaca aatgcgccgc caaacgcggt aagagtaaag cccgccatcg cccatccttt
    50581 gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc
    50641 cagccctaac cctggtttcc agataagccc gacaacgctc cacagcgatg gacgttctcc
    50701 cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta agggtaatac
    50761 cattgtggtg atcgccagtg cggcaaaacc gtaatggcta tgaatcaaca ggccaagcgg
    50821 cgcaccaaca gcgagggcac cgtaaatcgc cattccgttc catgacatca ctttgccaga
    50881 gtgttttggc cctacgatgc ctaaccccca ggtcagagcg cctgtcagta actggctttc
    50941 accaaaccca agaatcaaac gcccgataac caacagggca aatttgaacg gtgcggagac
    51001 aggcaaaatc gccgccagca gcaacgcgcc gccagccaga ccacaagcta acatcccctg
    51061 aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcgtaac cacgcgtcag
    51121 caccgtagcc agaaactgaa tcccgacggc aatgccgacc atggtattgc catagcccag
    51181 atcatgatga acaaacagcg ggataaccgg caacggcaac cctacggtca tgtaggtgag
    51241 aaaaaccgca aaagagatgc ggaagagcga aaaactggca gaagatcgtg tttcggtttg
    51301 gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta tccccgtctc
    51361 atctctctgg tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg tcttttcaga
    51421 aggaaatcga caaagcggga agtttgccta gaactggtgg cgattgtcaa tgatgtgaaa
    51481 aagggaacca tcaggttccc ttttgcgtta gtgccggagg ccgcattggc gcgtaacgtc
    51541 ggatgcgacg ctggcgcgtc ttatccgacc tactccggcc tactgcgaat taatcttttc
    51601 agcttgcgca ttaccagcgt ggcgttggtg ccgccgaagc cgaagctgtt agacataaca
    51661 gtggtcagtt cgcgatcggt cgtttcggtc acgatgttca gacccgcagc ctgctcgtcc
    51721 agctcttcaa tgttgatgct cggggcgata aagccgtgtt ccagcatcag cagagagtag
    51781 atagcttcct gcacacccgc agcgcccagt gagtgaccgg tcatggcttt ggttgcagaa
    51841 atcgccgggc tcttatcgcc gaacacttca cggatagctg ccagctcttt cacgtcgcca
    51901 accggagtcg aagtaccgtg agagttcagg taatcgattg gggtatcaac gccatgcatc
    51961 gccatctgca tacaacgtac tgcgccttcg ccagacggag caaccatgtc tgcaccatca
    52021 gaggttgcgc cgtagccaac gatttcagca tagatgtaag caccacgcgc cagcgcgtgt
    52081 tccagctctt caaccactac cataccgccg ccgccagcga taacgaaacc gtcacggtga
    52141 gcgtcgtaag tacgggaggc tttttccggg gtgtcgttgt atttagtaga tagcgcaccc
    52201 atcgcgtcga attcgcaggc catttcccag cacagctctt cgccgccgcc agcaaacacg
    52261 atgtcctgtt tgcccagttg gatctgctct actgcgttac cgatacagtg tgcggaagtc
    52321 gcacacgcgg agctgatgga gtagttaacg ccatgaattt taaacggcgt ggcgagacag
    52381 gcagaaacgc cggatgccat cgctttagtg accacatacg ggccaaccgc tttcaggccg
    52441 cgcgggccgc gcattgcgtc agcgccgaac acctggaaac gcggggaacc gccgccggaa
    52501 cctgcaatca ggccaacgcg cgggttattc tggtaagctt ccggagagag gcccgcatca
    52561 gcgattgcct gctccataga aaggaatgca taaatggatg cgtcgctcat aaagcgcaca
    52621 actttgcggt caatgaggcc agtggtatcc agttttatgt tgccccagac gtggctacgc
    52681 atgccggaat ccttcagctc ctgagagaaa gtgatccctg aacgtccttc acgcagagat
    52741 gccaggactt cctgctggtt attaccgatg ctggaaacaa tgcccaggcc agtaatcact
    52801 gcacgtttca ttcaatacct ctgtaagtcg cacatagagt aagtttcgaa tgcacaatag
    52861 cgtacacttg tacgccgaac aagtccgatc agccatttaa tagagatatt tgcgcagcct
    52921 tacacacatc gctaagatcg agccaccgcc tgtaagacga gtaacttacg tgaaacacta
    52981 ctccatacaa cctgccaacc tcgaatttaa tgctgagggt acacctgttt cccgagattt
    53041 tgacgatgtc tatttttcca acgataacgg gctggaagag acgcgttatg tttttctggg
    53101 aggtaaccaa ttagaggtac gctttcctga gcatccacat cctctgtttg tgatagcaga
    53161 gagcggcttc ggcaccggat taaacttcct gacgctatgg aaggcatttg atcagtttcg
    53221 cgaagcgcat ccgcaagcgc aattacaacg cttacatttc attagttttg agaaatttcc
    53281 cctcacccgt gcggatttag ccttagcgca tcaacactgg ccggaactgg ctccgtgggc
    53341 agaacaactt caggcgcagt ggccaatgcc cttgcccggt tgccatcgtt tattgctcga
    53401 tgaaggccgc gtgacgctgg atttatggtt tggcgatatt aacgaactga ccagccaact
    53461 ggacgattcg ctaaatcaaa aagtagatgc ctggtttctg gacggctttg cgccagctaa
    53521 aaacccggat atgtggacgc aaaatctgtt taacgccatg gcaaggttgg cgcgtccagg
    53581 cggcacgctg gcgacattta cgtctgccgg ttttgtccgc cgcggtttgc aggaagccgg
    53641 attcacgatg caaaaacgta agggctttgg gcgcaaacgg gaaatgcttt gtggggtgat
    53701 ggaacagaca ttaccactcc cctgctccac gccgtggttt aatcgtacgg gcagcagcaa
    53761 acgggaagta gcgattatcg gcggtggtat tgccagcgcg ttgttgtcgc tggcgttatt
    53821 acggcgcggc tggcaggtag cgctttattg cgcggatgaa gcccccgcgc tgggtgcttc
    53881 cggcaatcgc cagggggcgc tgtatccgtt attaagcaaa cacgatgagg cgctaaaccg
    53941 ctttttctct aatgcgttta cttttgctcg tcggttttac gaccaattac ccgttaaatt
    54001 tgatcatgac tggtgcggcg tcacgcagtt aggctgggat gagaaaagcc agcataaaat
    54061 cgcacagatg ttgtcaatgg atttacccgc agaactggct gtagccgttg aggcaaatgc
    54121 ggttgaacaa attacgggcg ttgcgacaaa ttgcagcggc attacttatc cgcaaggtgg
    54181 ttggctgtgc ccagcagaac tgacccgtaa tgtgctggaa ctggcgcaac agcagggttt
    54241 gcagatttat tatcaatatc agttacagaa tttatcccgt aaggatgact gttggttgtt
    54301 gaattttgca ggagatcagc aagcaacaca cagcgtagtg gtactggcga acgggcatca
    54361 aatcagccgt ttcagccaaa cgtcgactct cccggtgtat tcggttgccg ggcaggtcag
    54421 ccatattccg acaacgccgg aactggcaga gctgaagcag gtgctgtgct atgacggtta
    54481 tctcacgcca caaaatccgg cgaatcaaca tcattgtatt ggtgccagtt atcatcgcgg
    54541 cagcgaagat acggcgtaca gtgaggacga tcagcagcag aatcgccagc ggttgattga
    54601 ttgtttcccg caggcacagt gggcaaaaga ggttgatgtc agtgataaag aggcgcgctg
    54661 cggtgtgcgt tgtgccaccc gcgatcatct gccaatggta ggcaatgttc ccgattatga
    54721 ggcaacactc gtggaatatg cgtcgttggc ggagcaaaaa gatgaggcgg taagcgcgcc
    54781 ggtttttgac gatctcttta tgtttgcggc tttaggttct cgcggtttgt gttctgcccc
    54841 gctgtgtgcc gagattctgg cggcgcagat gagcgacgaa ccgattccga tggatgccag
    54901 tacgctggcg gcgttaaacc cgaatcggtt atgggtgcgg aaattgttga agggtaaagc
    54961 ggttaaggcg gggtaatctg ctctcgcagt gtttgccgga tgcggcgtga acgccttatc
    55021 caacctacgt gtgacgtgta ggcctgataa gacgcgataa gcgtcgcatc aggcatggtg
    55081 ctccagacgc cgcaacttac agctgtgacg cctgctgaaa caggttttcc cacatatcgg
    55141 tcaccagcgc ctggtcacgc ggcgacaatt caccggcacc aatggctttt tccagactct
    55201 ggctaacggt cgtatgcacc gcctgagcgg agtggtcatc accactttcc agttctgcga
    55261 tggctaatgt caggtggcca cgcaaatacc cactggcaaa caactcatca tcactggcat
    55321 ggtcaaccat gccgtcgatt aatgccagaa tgcgtgattc aaactccgcg atcatcttct
    55381 ttcctcattg taaaacgtgg cgctgacttc agttgagcgc ttccggccac gggaactgtt
    55441 ccgccgtaag ttccggcgtg tgataataat tttgtagtgc tttaatgaag cgtgccgggc
    55501 gttccggaat gccatttgtc agataatcca tcacctgcgc atgtacccgc cgttgaaaca
    55561 ccacacgatc cggttcgaaa tccccttcca gattgtcgca actgacgtta aacggatatc
    55621 cagccgccac gcagaataac cagtcgaacg cctgcggctt cacttcaaca tcttcaaact
    55681 ggctttgcgt ctgggcatcg cgtccgtccg ggcaatacca gtagccgaaa tcaaccagct
    55741 cacggcgcgc ttttccggca atacaccagt gcgaaatctc atgaattgcg ctggcataaa
    55801 agccatgagc aaagacgatt cggttatacg gtacttccgc atcagcagga agatagatcg
    55861 gttcgtcgtc gcctttaatc agacgggtat taaattcatc ggcaaagcag ctattaaaaa
    55921 tttcaatcaa ctgctcgtag tggtgtgtac tgttcattag ttcatcccca accagtggag
    55981 gatctcctgt ccgtggctat cgtaaagtaa tttggcactc atcaccgccg agacgataac
    56041 aatcatcggg cggatcagct tttgtccttt gctcaacacc aaccgtgaac ccatgcgcgc
    56101 gccgaggaac tgccccacca gcatgacaaa gcccgtcgcc caaatcactt tgccgccgag
    56161 aataaacagc agcagaccgc caatgtttga cgttgcgttg agtaatttgg cgtgagccgt
    56221 ggctttggcg aggttgaatc cgcacagcat aacgaaggcc agcgcgtaaa acgatccggc
    56281 agccgggcca aagaatccgt cataaaaacc gacgcagcca ccggcaatta acgcgaaggg
    56341 taaaccgtac atccggcgct ggcgatcttc ttcacccagc tttggcatca gcaagaaata
    56401 gaggccgata caaatcacca gaatgggcaa aatctgccgt aagacatcag cctgaacgta
    56461 ctgtaccaac agtgcgccgc tcattgagcc gacaaaggtc atggcaatat tgagtttctg
    56521 atcgcttaaa ctaaccactt tgcggcgaat aaagtagata gtggcggaaa tagagccgcc
    56581 gcaggcttgc agtttattgg ttgccagcgc gttagcggga gacattcccg cagccatcaa
    56641 tgccggaatg gtgagtaacc caccgccacc ggcaatcgag tcgataaatc ccgccagcat
    56701 ggcgacaaaa aagagaactc ccagcaacag cggagaaacc ataaacaggc tattaaacgt
    56761 ttccattaga tcacgtgctc atccagtagc gcctggcagg aaggcggcaa cggaggcggt
    56821 gtcttcttct caggctttgt tgttcccggt tttggaggtt caaaccagct ttgcagttct
    56881 gccccgcaac catcgcctga tggcggtaaa ggttgatctt cacactccag actatcggca
    56941 gggcaacgta atcgtacatg catatgcgcg cgatgctgga accagggtcg cactttgcgc
    57001 aaccagtcgc gatcggtgcc cgcatcaagg caaagttgtt gtttaatcgc cggattaaca
    57061 aaaatgcgcg tgacgtcttt gtcctgggcg gcgagtttga tcaagctgaa aatttctggc
    57121 ttccacagcg tggagacaac gtgtttaccg tcgcgggaaa ctaagtccag tgcttgcggg
    57181 cgcaagagct gcgcggaggt ccagcgagtt ttcggcagtt gcagaaagat atcgacatcc
    57241 agtccggtct ggtggctggc atgaccgccg ttgaaacgcc caccagcggg catccccata
    57301 tcgccaatca gcaccgtacc catgcccaga ttgctcacct ggctactcag acgctggata
    57361 aacatcacca gatccgggtg accgaaatag cgacgctgat cggtacgcat gacctgataa
    57421 tgttcggact gtatcggcaa cgtgtcagcg ccgacaatac agccattaga aaaactgcct
    57481 atcgattgtg cgctacccgg cacaggttgg gttatttttt gccacggcgt cgctgccagg
    57541 ctagcgctac tggcaagcag agccagcagc gcaatcgcgg ttttgttcat tttttaccag
    57601 cgtggaatat cagtcttcac atcggcattt tgcgcccgct gccgtaacag gtgatccatt
    57661 aaaacgatcg ccagcatcgc ttcagcgatc ggcactgcac ggatcccgac acagggatcg
    57721 tgacggcctt tggtgatcat ctcaacttct tcgccaaagc ggttaatagt gcgccccggc
    57781 acggtaatgc tggaggtcgg tttcagcgcc atatgagcaa tgatttgctg cccgctgctg
    57841 ataccgccga aaatgccgcc cgcatggttg ctctggaaac cgtctttggt gatttcgtcg
    57901 cggttctggc tgccgcgcag cgccactacg tcaaaaccgt cgccaatttc cacgcctttc
    57961 accgcgttga tgctcatcag cgcatgggcg atgtcggcat ccaggcggtc aaagaccggc
    58021 tcgccaagtc cggcggggac accactggca acaacggtga ctttcgcgcc gatggagtcg
    58081 ccctcttttt tcagcgcgcg catcagttca tctaacgcgt cgattttgtc ggggtccggg
    58141 cagaagaacg gattttgttc aaccagcgac cagtctttga tttccagcgg aatgtcgccc
    58201 atctgggtca ggcagccgcg gatttcaata ccaaatttct cggcgagata ttttttggca
    58261 atcgcccctg ccgccacgcg catggcggtt tcgcgggcgg aagaacgtcc accgccgcga
    58321 taatcgcgca gaccgtattt ttgttcgtag gtgtaatcag catggcctgg acggaagacg
    58381 tctttaattg cgctgtaatc ctgagaacgc tggtcggtat tttcgatcaa caagccaatg
    58441 ctggtgccag tagtaacgcc ttcaaaaaca ccggagagta ttttgacctg atccggctcg
    58501 cggcgctggg tggtatagcg cgatgtccca gggcgacgac gatcaaggtc atgttgcagg
    58561 tccgcttccg tcagcggaat gcctggcgga acaccatcga cgatgcagcc gagcgccagc
    58621 ccgtgcgatt cgccgaaggt ggttacgcga aagagttgtc caattgtgtt tccagccatc
    58681 acggctccgt tattgttgtg tttgcgtgtt tacttaatct ttataaatcg cgaaatgttc
    58741 tcgtgcggca ataagctgct ctttagtgag cataaacaca ccatcgccgc cgttatcaaa
    58801 ctccagccag gtgaacggaa catcgggata ttgttccata agatgtacca tgctgttgcc
    58861 cacttcacaa atcaacacgc catcatcagc aaggtaatct gccgcgttac cgagaatgcg
    58921 acgcgtcagt ttcaggccgt cggtgccaga tgccaggccc agttccggct cgtgacggta
    58981 ttcgtttggc aggtcggaca tatcttccgc gtcgacatac ggcgggttag tgacaatcag
    59041 atcgtattgc actttcggca aatcacggaa cagatcggaa cgaatcggaa tgacgttgtg
    59101 gatcagaccg tgttcttcga tgttctgttc ggcaaccgcc agcgcgtcgg gagagatatc
    59161 taccgcgtcg acttccgctt ccgggaaggc ataagcacag gcaatggcga tgcagccgct
    59221 accggtacac atatctaaaa tatgctgcgg ttgcttgctg atgagtccgg caaatttatt
    59281 gttgatcagt tcaccaatcg gcgagcgcgg caccagcacg cgttcatcga cgtaaaattc
    59341 atgaccgcag aaccaggctt tgttggtcag gtaagccacc ggaatgcgct cgttgacgcg
    59401 gcggatcacg cgttcaacaa tacggtgttt ttcgctggag gtcagacgcg cggtgcgcat
    59461 atcttccggg atatccagcg gcaggtagag cgatggtaac accagctgta cggcttcatc
    59521 ccacgggtta tcggtgccgt gaccgtacca gatatttgcc gcgctgaagc ggcttaccga
    59581 ccagcgcaac atgtcctgaa tggtttgcag ctcatttact gcttcatcaa cgaaaatttt
    59641 atccacgtat tcctccaggg catgatcgca ataatttcgg cggctagttt gccatgaaga
    59701 tgacgataaa tcagcattca cgcgcggtga gtgaggaaaa atacgtttaa aacgatcgat
    59761 tgtgctacga gtcgggtaaa ctgtaggaaa attagaaata gagacagata aatgaaaaag
    59821 aaaacaacac ttagcgagga ggaccaggct ctgtttcgcc agttgatggc ggggactcgc
    59881 aagattaagc aggacacgat tgtccaccga ccgcagcgta aaaaaatcag cgaagtgccg
    59941 gtgaaacgct tgatccagga gcaggctgat gccagccatt atttctccga tgagtttcag
    60001 ccgttattaa ataccgaagg tccggtgaaa tatgttcgcc cggatgtcag ccattttgag
    60061 gcgaagaaac tgcgccgcgg cgattattca ccggaattgt ttttggattt acacggtctg
    60121 acgcagctgc aggccaagca ggaactgggc gcgttgattg ccgcctgtcg ccgcgaacat
    60181 gtgttttgtg cctgcgtgat gtatgggcac gggaagcata ttttgaagca acaaacaccg
    60241 ctgtggctgg cacaacatcc gcatgtgatg gcatttcacc aggcaccgaa agagtacggc
    60301 ggtgatgcgg ctttgttggt gttgattgaa gtggaagagt ggttgccgcc ggagttgccc
    60361 tgaatgctgg cagcaaatgc cggagcagcg taaacgcctt atccggccta caaatatgtg
    60421 ataattcaat aaattgcaat tcacttcgta ggcctgataa gacgcatcag agcaatacta
    60481 cgaacttcag ttcattcaat acatactgaa aacgacacgt cgaatcaatc ggttgcccac
    60541 tactggtatt cacattcacc acaatatcgt gtagtacttt atccgttacc ggatgacgag
    60601 ttggcgtaac cgttaccgtt gatttatcac aaccgtaaga ggtaaaggtt aacaattctg
    60661 ataccgcgcg gcagtatgct cctctgccat cattatcaga aaaattactg ccatcacaga
    60721 gtctgccgcc ttccatcagg taggtcgaaa gcgtggtggt cactgttgac cccgtggact
    60781 gaattcgtaa cgccttatta tggctgacat aattttcact ccaggagata tcgtatgaag
    60841 cgaccggtat cgttgggcat attgtgttat gcactgtagg ggccgtatag cttccaaatc
    60901 cgggtaaatc tgtataggaa taaaaggatg cgatccaagg gcaaccataa tcatcagatg
    60961 caacggtaat tttaaacgtc atatatgcac aacgaaaaat tttcgtatct atgccagggc
    61021 aatttgagtt attaattgta tcaaagtcag tcagtaccaa agttggagct gaacccgacc
    61081 cagaccagga aataacccgc aacgtatcgc ctttatacgc aaaagaatca tatttcccat
    61141 atgcaacctc ctttggcgtt atcaccgtaa cattgaaaac actgctcgct acagcaccgg
    61201 ttaccttgtc catggcataa taaactctca cactgtcaaa actcgcactc ataagtttat
    61261 ctccagcata actcttagat gcgccgacaa cactcagaac gagcagcatt aggctcgcga
    61321 aaattatggt ccattttttc atttcagaaa tactcaaagg ttgccgtcac tgtggcacta
    61381 aagtctccgg acgttacatc tcgcccgctc tttgcctgta accaggtatt aaaatttagg
    61441 ctgttatttc cctgattcag cgggagcgtg acgcctgttg tattgttgat tggcaccgat
    61501 gtgcctgccg cagtttcaat accaatccca gcgccggatg ctgatgacga ggagtcaaag
    61561 gccagaaagc cgggtagagc actatcttct gttcctgtca acgttacttt gacattaaac
    61621 aatgtagaac tatgacaatc tttcaattga aacaccaatg gcactcgctc tgactgccca
    61681 aggttcatta aatcactggc ggcaatggca ggaaaatgta cttctgccaa ctggctacca
    61741 ttaatcactg gcgtacacga tttactgatt aaattgccgg taaagtgcaa attatctcct
    61801 gctattgtgg ttccactgcc cagcaacatc aggctactaa taaaaaacaa cgtacgcttt
    61861 ttcttcattg gtaatctacc tgtaacgtgg cccaggcctc aaagtccgcc tctggcagaa
    61921 tgacaccact cttttttact ggcacagccg ttaacaccgg taaatcggtt tcattcacca
    61981 caagtggcgt attgatcgta aaactctgcc ctgcctgttt gagctgaatg cctaaaccag
    62041 tgatattgct gcttactgca gcattatcaa aatcgcttgg tgtgccactc cagctcaagg
    62101 tcatttccat taatgggtcg cgaaccgccg tatcgcaggt aatggaatac ggcacggttt
    62161 gctcataatt gctgccatta atgtcatcaa taagcacatc ctgaaaagtt atttcaatgg
    62221 tttgagcgtt gctaatcgtg cagttaggcg gctgcactaa ataaccgtga aaagagacgt
    62281 ttgagagtgc catcgagcaa aagccccaca gcaaaataag cgagatccgt ttcattggta
    62341 atccaccaca attgtggcac tggcgttaaa ttcagctgcc gctaacgttg taccactctg
    62401 tttcaccggc acagcttcca gaacgggaac tcccgaggaa aaattaaatg gcaaccatga
    62461 gccagaggtt aagtcgagta ttgtgtgatc actacttttc tgaacccgaa taccaaaccc
    62521 aggaataccc gtgctgataa ctgtttcacc gttaatcacg accgttgtcg cctgcatctg
    62581 tagacgcagg tcatccattg ccagattgtt acaaacaagc gagtagtcaa tgggacgttt
    62641 ataatcaacc ctattgatct tattaatgaa tacattacca aactcaactg ccgcaccatt
    62701 taccgtacaa ggaggagggg catcgacaag cacccgtaaa ttcatactta ctgacttact
    62761 cgctgcaact gcaaagccgc taccaacaca acatatcagt aacccagtta ccgcccagac
    62821 agaacaagat cctttcatac tggctctccc ggcctcagcc tttgcggctc tgctcttcat
    62881 cgacctagca actgttaccg ttgcaagtga aaaagagcgg catccgagcg ccatagtcgt
    62941 tgacataggt cagtaccggc acgctaccca tcttcacatt cagcggtgca gaggttttcg
    63001 gcgacatcac tattggcgag aaaccggctg ccggattgcc atttttctgt gtgctggcat
    63061 tactgaaaat gacgtaatac gacgtcgggt tattgacggt aaattcctga ccgttgcggg
    63121 ttaaagtaat cttatattgc taggggtttt tcatgctgac gttctccagc gccttcggtc
    63181 gccagaacaa cttaatacgc gtttgtagtg ctatttgcag cgtgttggct ttgtttgatt
    63241 ttggcgggat ctcacgcacg ttgaagtaga acaggctttc acgatcggca ggcaatttgt
    63301 tgatatccgg cattccctgc acttttacct gcccgttcat cattgaatcg atgcgttgca
    63361 ccggtggcag aacagttaac ggtgaactga tcttgttacc cttttcatct tcgatccagc
    63421 tttgggccag ataaggcagt ttcggatcgt tattgcgcaa cgtcacgctg attgatttgt
    63481 cgctttcatt aaatatcaaa cgggtacgat ctggcgttac agaagcctgg gcagacaaac
    63541 tggcagcgct aagcagtaac gctagcgtcg tagcacccaa tttggcagag caaagtaaat
    63601 cagacatgga tcattcctta atcacgttat tggttagctg aaacagaaac agggggattg
    63661 gtgggtgtca cctgttgcgt ctgctcctga atgacaggct ttatgtcatc aggaacaacc
    63721 ggcgcgacgt tgcctttatg ctggcatggc agtaacaagc cgttaaacag atcggcaggc
    63781 agcgggtccg gcaggttgat atcgcaatgc gcaacaccac tccagaacac actcatgtgt
    63841 tcgccaggtt tcaccccagc cagataaaca ttgccgtcat catcgacaag gcccactgtc
    63901 tgctcgttat cattttttac ttctgcgcca aacggaggat ggctgccgtc ttgtaaacgc
    63961 agcaccgcca tcgctttttg accactgatg acggcaaatt tgcggtagcc aatcgcccct
    64021 tcagttagcg tggcttgtac caccgactgg gttgcttcgg cgttttccgg cagtttgttg
    64081 aggtcgatat acgcctgatt gcggtaatag ttattgacgt cagaaacgac ggctttacca
    64141 aacatattgg tataaacagc agcaccgtta ccttctaccg gaacatcggc aacgccatcg
    64201 gcatcaatca acaagcgtgt cccgcccata ttctgggtac ggtgaagtgc gccaccgtgg
    64261 gcagtaagcg ttgcgccgcc ctgtaacgac aagcccgcag aggtgtattg cccttcatgg
    64321 tagttcgcac tgaggtcaac ctgcgccagc gaaccatcat ggctgtaata gccgtcaacg
    64381 ctggtgtgtt tgtcactggt gccgacgttc aactgatagt gagtcgcgtc atcgacacgg
    64441 ctgaaataac cgacctgact gctgtccgtc ccactgccat agtaccgtta tagctaacgg
    64501 tactgttgtc gccccacggc atactgagcg aaatgtacat gcctttgtcg gcctggttgt
    64561 catactcgta acggtagcca gtcatcgaga tgctgacatt acgaatactg cccatattga
    64621 aatagtgcga gagcatgatg ttgtagtttg tctgctcctc gcgatcccag taggtatggc
    64681 gggtatagtt gagataaacc gaaacacccg catcgcggaa gttctggtta taagtggcgg
    64741 tgtacatctc tttgtcgttg cccgtgcgta ccattccgct gtcactggca tccagatact
    64801 cgctcatggt cataaagttc tcttccgaga agcgatatcc agcaaaagta acgcggctgt
    64861 tgagctggtc aaagtctttg gaataactca cacggaagga gttaccgtcc agcgaacctt
    64921 tgccataagc ggtgtcttta tccagtttgg tgtgcgagtg agtaacatca aacgcgaccg
    64981 cgccgaatgt agacaaatcg cgaccgacac caagcgccgc agactgatag ttttcatctc
    65041 ccagtgcgcc gccatataac gaccagccgt tagcgatccc ccaggaagct tcagcaccag
    65101 aaaaaaattc accctcgaca tggtgtcccc actcttgcgg acggcccatc atgatcttat
    65161 agcgaacctg acctggacga gtgaggtatg gcatcgaggc ggtgctgatg tcatattcct
    65221 gcacctggcc gttctgttct tcaatgcgaa tatgcaacgt accggagaca gaatcaccaa
    65281 gatcctgaat acgaaacggt ccagccggaa cctgcgtttc gtaaatcaca cgccccatct
    65341 ggctgacggt cacttttgct gtggtgtgtg ccacgccgga aatgtctggc gcgtagccgc
    65401 gcagattggg aggcaacatt tgatcgtcag tactgacact gccaccaaca tagttaaaac
    65461 catcaaaaat atcggatctg aggtaatcct cgcccagcgc cagtttggct tttaatgatg
    65521 gtaacgcccg cctggcatag tagcgac
//
LOCUS       DAJVPP010000007        64930 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVPP010000007 DAJVPP010000000
VERSION     DAJVPP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.007"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1264)
                     /gene="pcnB"
                     /locus_tag="OQM34_000465"
     CDS             complement(<1..1264)
                     /gene="pcnB"
                     /locus_tag="OQM34_000465"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1451038.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNA"
     gene            complement(1303..2229)
                     /gene="gluQRS"
                     /locus_tag="OQM34_000466"
     CDS             complement(1303..2229)
                     /gene="gluQRS"
                     /locus_tag="OQM34_000466"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1451039.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2266..2721)
                     /gene="dksA"
                     /locus_tag="OQM34_000467"
     CDS             complement(2266..2721)
                     /gene="dksA"
                     /locus_tag="OQM34_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1451040.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(2899..3603)
                     /gene="sfsA"
                     /locus_tag="OQM34_000468"
     CDS             complement(2899..3603)
                     /gene="sfsA"
                     /locus_tag="OQM34_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1451041.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(3618..4148)
                     /gene="thpR"
                     /locus_tag="OQM34_000469"
     CDS             complement(3618..4148)
                     /gene="thpR"
                     /locus_tag="OQM34_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1451042.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4222..6651
                     /gene="hrpB"
                     /locus_tag="OQM34_000470"
     CDS             4222..6651
                     /gene="hrpB"
                     /locus_tag="OQM34_000470"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1451043.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            6847..9381
                     /gene="mrcB"
                     /locus_tag="OQM34_000471"
     CDS             6847..9381
                     /gene="mrcB"
                     /locus_tag="OQM34_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1451044.1"
                     /translation="MAGNDREPIGRKGKLTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            9601..11844
                     /gene="fhuA"
                     /locus_tag="OQM34_000472"
     CDS             9601..11844
                     /gene="fhuA"
                     /locus_tag="OQM34_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1451045.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            11895..12692
                     /gene="fhuC"
                     /locus_tag="OQM34_000473"
     CDS             11895..12692
                     /gene="fhuC"
                     /locus_tag="OQM34_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1451046.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            12692..13582
                     /gene="fhuD"
                     /locus_tag="OQM34_000474"
     CDS             12692..13582
                     /gene="fhuD"
                     /locus_tag="OQM34_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1451047.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELMTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            13579..15561
                     /gene="fhuB"
                     /locus_tag="OQM34_000475"
     CDS             13579..15561
                     /gene="fhuB"
                     /locus_tag="OQM34_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1451048.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(15596..16876)
                     /gene="hemL"
                     /locus_tag="OQM34_000476"
     CDS             complement(15596..16876)
                     /gene="hemL"
                     /locus_tag="OQM34_000476"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1451049.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17101..18522
                     /gene="clcA"
                     /locus_tag="OQM34_000477"
     CDS             17101..18522
                     /gene="clcA"
                     /locus_tag="OQM34_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1451050.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            18546..18611
                     /locus_tag="OQM34_000478"
     CDS             18546..18611
                     /locus_tag="OQM34_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451051.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            18604..18948
                     /gene="erpA"
                     /locus_tag="OQM34_000479"
     CDS             18604..18948
                     /gene="erpA"
                     /locus_tag="OQM34_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1451052.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(18995..19618)
                     /locus_tag="OQM34_000480"
     CDS             complement(18995..19618)
                     /locus_tag="OQM34_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1451053.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGEIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(19656..20456)
                     /gene="btuF"
                     /locus_tag="OQM34_000481"
     CDS             complement(19656..20456)
                     /gene="btuF"
                     /locus_tag="OQM34_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCS1451054.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20449..21147)
                     /gene="mtnN"
                     /locus_tag="OQM34_000482"
     CDS             complement(20449..21147)
                     /gene="mtnN"
                     /locus_tag="OQM34_000482"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1451055.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21231..22748
                     /gene="dgt"
                     /locus_tag="OQM34_000483"
     CDS             21231..22748
                     /gene="dgt"
                     /locus_tag="OQM34_000483"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1451056.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            22878..24302
                     /gene="degP"
                     /locus_tag="OQM34_000484"
     CDS             22878..24302
                     /gene="degP"
                     /locus_tag="OQM34_000484"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1451057.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24457..25613
                     /gene="cdaR"
                     /locus_tag="OQM34_000485"
                     /pseudo
     CDS             24457..25613
                     /gene="cdaR"
                     /locus_tag="OQM34_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(25702..26088)
                     /locus_tag="OQM34_000486"
     CDS             complement(25702..26088)
                     /locus_tag="OQM34_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1451058.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26402..27226)
                     /gene="dapD"
                     /locus_tag="OQM34_000487"
     CDS             complement(26402..27226)
                     /gene="dapD"
                     /locus_tag="OQM34_000487"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1451059.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27257..29929)
                     /gene="glnD"
                     /locus_tag="OQM34_000488"
     CDS             complement(27257..29929)
                     /gene="glnD"
                     /locus_tag="OQM34_000488"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1451060.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVHQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(29991..30785)
                     /gene="map"
                     /locus_tag="OQM34_000489"
     CDS             complement(29991..30785)
                     /gene="map"
                     /locus_tag="OQM34_000489"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1451061.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31153..31878
                     /gene="rpsB"
                     /locus_tag="OQM34_000490"
     CDS             31153..31878
                     /gene="rpsB"
                     /locus_tag="OQM34_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1451062.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32136..32987
                     /gene="tsf"
                     /locus_tag="OQM34_000491"
     CDS             32136..32987
                     /gene="tsf"
                     /locus_tag="OQM34_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1451063.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33134..33859
                     /gene="pyrH"
                     /locus_tag="OQM34_000492"
     CDS             33134..33859
                     /gene="pyrH"
                     /locus_tag="OQM34_000492"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1451064.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34151..34708
                     /gene="frr"
                     /locus_tag="OQM34_000493"
     CDS             34151..34708
                     /gene="frr"
                     /locus_tag="OQM34_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1451065.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            34800..35996
                     /gene="ispC"
                     /locus_tag="OQM34_000494"
     CDS             34800..35996
                     /gene="ispC"
                     /locus_tag="OQM34_000494"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1451066.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36185..36943
                     /gene="ispU"
                     /locus_tag="OQM34_000495"
                     /gene_synonym="uppS"
     CDS             36185..36943
                     /gene="ispU"
                     /locus_tag="OQM34_000495"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1451067.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            36956..37813
                     /gene="cdsA"
                     /locus_tag="OQM34_000496"
     CDS             36956..37813
                     /gene="cdsA"
                     /locus_tag="OQM34_000496"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1451068.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            37825..39177
                     /gene="rseP"
                     /locus_tag="OQM34_000497"
     CDS             37825..39177
                     /gene="rseP"
                     /locus_tag="OQM34_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1451069.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39207..41639
                     /gene="bamA"
                     /locus_tag="OQM34_000498"
     CDS             39207..41639
                     /gene="bamA"
                     /locus_tag="OQM34_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1451070.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            41761..42246
                     /gene="skp"
                     /locus_tag="OQM34_000499"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             41761..42246
                     /gene="skp"
                     /locus_tag="OQM34_000499"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1451071.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42250..43275
                     /gene="lpxD"
                     /locus_tag="OQM34_000500"
     CDS             42250..43275
                     /gene="lpxD"
                     /locus_tag="OQM34_000500"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1451072.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQSNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43380..43835
                     /gene="fabZ"
                     /locus_tag="OQM34_000501"
     CDS             43380..43835
                     /gene="fabZ"
                     /locus_tag="OQM34_000501"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1451073.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            43839..44627
                     /gene="lpxA"
                     /locus_tag="OQM34_000502"
     CDS             43839..44627
                     /gene="lpxA"
                     /locus_tag="OQM34_000502"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1451074.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44627..45775
                     /gene="lpxB"
                     /locus_tag="OQM34_000503"
     CDS             44627..45775
                     /gene="lpxB"
                     /locus_tag="OQM34_000503"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1451075.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            45772..46368
                     /gene="rnhB"
                     /locus_tag="OQM34_000504"
     CDS             45772..46368
                     /gene="rnhB"
                     /locus_tag="OQM34_000504"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1451076.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46405..49887
                     /gene="dnaE"
                     /locus_tag="OQM34_000505"
     CDS             46405..49887
                     /gene="dnaE"
                     /locus_tag="OQM34_000505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1451077.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            49900..50859
                     /gene="accA"
                     /locus_tag="OQM34_000506"
     CDS             49900..50859
                     /gene="accA"
                     /locus_tag="OQM34_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1451078.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            50958..53099
                     /gene="ldcC"
                     /locus_tag="OQM34_000507"
     CDS             50958..53099
                     /gene="ldcC"
                     /locus_tag="OQM34_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1451079.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAASSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYHVRVLKMAG"
     gene            53156..53545
                     /locus_tag="OQM34_000508"
     CDS             53156..53545
                     /locus_tag="OQM34_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1451080.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53610..54908
                     /gene="tilS"
                     /locus_tag="OQM34_000509"
     CDS             53610..54908
                     /gene="tilS"
                     /locus_tag="OQM34_000509"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1451081.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(54957..55217)
                     /gene="rof"
                     /locus_tag="OQM34_000510"
     CDS             complement(54957..55217)
                     /gene="rof"
                     /locus_tag="OQM34_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1451082.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55204..55404)
                     /locus_tag="OQM34_000511"
     CDS             complement(55204..55404)
                     /locus_tag="OQM34_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1451083.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55570..56115
                     /locus_tag="OQM34_000512"
     CDS             55570..56115
                     /locus_tag="OQM34_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1451084.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56112..56534
                     /gene="arfB"
                     /locus_tag="OQM34_000513"
     CDS             56112..56534
                     /gene="arfB"
                     /locus_tag="OQM34_000513"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1451085.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56548..57258
                     /gene="nlpE"
                     /locus_tag="OQM34_000514"
                     /gene_synonym="cutF"
     CDS             56548..57258
                     /gene="nlpE"
                     /locus_tag="OQM34_000514"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1451086.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(57413..58237)
                     /locus_tag="OQM34_000515"
     CDS             complement(57413..58237)
                     /locus_tag="OQM34_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCS1451087.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58290..60008)
                     /gene="proS"
                     /locus_tag="OQM34_000516"
     CDS             complement(58290..60008)
                     /gene="proS"
                     /locus_tag="OQM34_000516"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1451088.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLIKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60119..60826)
                     /gene="tsaA"
                     /locus_tag="OQM34_000517"
     CDS             complement(60119..60826)
                     /gene="tsaA"
                     /locus_tag="OQM34_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1451089.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(60823..61227)
                     /gene="rcsF"
                     /locus_tag="OQM34_000518"
     CDS             complement(60823..61227)
                     /gene="rcsF"
                     /locus_tag="OQM34_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1451090.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61345..62160)
                     /gene="metQ"
                     /locus_tag="OQM34_000519"
     CDS             complement(61345..62160)
                     /gene="metQ"
                     /locus_tag="OQM34_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1451091.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LIAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62200..62853)
                     /gene="metI"
                     /locus_tag="OQM34_000520"
     CDS             complement(62200..62853)
                     /gene="metI"
                     /locus_tag="OQM34_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1451092.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(62846..63877)
                     /gene="metN"
                     /locus_tag="OQM34_000521"
     CDS             complement(62846..63877)
                     /gene="metN"
                     /locus_tag="OQM34_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1451093.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64065..64640
                     /gene="gmhB"
                     /locus_tag="OQM34_000522"
     CDS             64065..64640
                     /gene="gmhB"
                     /locus_tag="OQM34_000522"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1451094.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15339 a        16097 c        17914 g        15580 t
ORIGIN      
        1 cagcattacg ttcaacttca gctcgcaagg ccaacaggtc ataagccgca cggaacttag
       61 gatgctccag cagtttccat gcgcgtttac cctgacgacg ggacatacgc aactgcaact
      121 gccagatatc gcgggttaac gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt
      181 cgtccagcac gtcgttcatc gccagcgcga aagcgtcgtg ataggtcagg ccgctttcct
      241 gggcgatctt ctgtgccgtc tccagcagtg ggtaccagaa catggcggca aacaagaacg
      301 ccgggttcac gcgcatatcg ttatggatac gcgtatcggt attcttcagc acctgttcaa
      361 tgatccgctc catcgggctg tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca
      421 gcggctggaa cagatgatat tcacacaaca gcttataggt ttcgtaaccg tagcccgctt
      481 gtagcagttt aagcgattct tcaaacaggc gtgccggtgg gatatcgttc agcagggtag
      541 cgaggcgagg gatcggttct gcggtttccg ggctgatgcg catacccaat ttggcggcaa
      601 aacgtaccgc gcgcagcata cgtaccggat cttcacggta gcgcgtttcc gggttaccaa
      661 tcagacggat aacgccgtcc ttcagatcct tcataccacc aacgtaatca cggacggtaa
      721 aatccgctac gctgtaatac aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt
      781 cgatggagcc gaaaatgttg tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg
      841 tgcggtcgct gacgttacct tcgtggtgtc cacggaaggt cgcaacttcg ataatctccg
      901 ggccaaacat tacatgagcc agacggaaac ggcgacccac caggcggcag ttacggaaca
      961 gtttgcgcac ctgctcaggc gtggcgttag tggttacgtc aaaatctttc ggctttttgc
     1021 caagtaacag gtcgcgcacg ccgccgccaa ccagccaggc ttcgtatccc gctttgttga
     1081 gcctgtacat taccttcagg gcattttcac tgatatcttt gcgggaaata gcatgctgct
     1141 cacgcgggat caccgtcacc tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc
     1201 ggcttagcac cttgcggcaa aaattagcga ctcgggtaaa aatagtacac ctcggtagtg
     1261 tcaaacatca ttcaggacaa aaaaatagcg gctaatcata gctcagcatg acgcatttga
     1321 gaatgttgaa tttacaattg ccgactcggg cacggcggtc agcatccagt ttttgacggc
     1381 tgactgaagg atttgctcga cgctgaaatc ctgccagtgt gcttctgcct gctgccccag
     1441 aaattgaagt gccgcgatta gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg
     1501 attctgcttg gaaagtttag cgccttgtgg attaagcgcc agcggcagat gaatgtaatc
     1561 tggcactttc cagccaaaaa gctggtacag cgggatttgc cttactgttg gttcaatcag
     1621 atcagcccca cgcactattt ctgtaacgcc ctggaaatga tcatcaacca caacagccag
     1681 gttgtaggcg aacaacccat cacggcgatg aatgataaaa tcttcccgtg ccagtttttc
     1741 gtcggcgtga ataatgcctc gcagcaggtc agtaaattgc gtgaccggat gctgctggcg
     1801 gatacgcact gcggcgttgt ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat
     1861 accgccaatg ctttgaatac gcgcacgcgt acaggtgcag taataactta gtccttgttc
     1921 atgtaaccag gcgagtgctt cacgataggc gtcgtgacgt tgcgattgcc agagaacatc
     1981 gccgtcccag tgcagaccgt aatgttccag ctggcgcagg atagtttctg cggcaccggg
     2041 aacttcacga ggcgggtcga tatcttctat gcgtaccagc cagcgacctt gccgggcgcg
     2101 agcctgcaaa tagctgccga gcgcggcgat cagagagcca aaatgaagct cgccggaagg
     2161 agagggggcg aagcggccaa tatactgtgt gtctgtcatc tctttgaaca aaaaataagg
     2221 cgggagcatt tcccgcctgt ggtaaacgtg atggaacggc tgtaattagc cagccatctg
     2281 tttttcgcga atttcagcca gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc
     2341 ttccagacgg cgaataccaa tttcaacacc gcaggattcg cagtagccga aatcttcgtc
     2401 ttccactttt ttcagcgtct tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt
     2461 acgcagttcg aggctgaact cttcttcctg ggctgcacgg tctaccgggt ccgggaagtt
     2521 ggctgcttca tcctgcatat gtgtaacggt gcgatcgact tcatccctga gttgattacg
     2581 ccatgcttcc agaatacgac ggaagtgcgc cagctgggct tcattcatat actcttcgcc
     2641 cggcttctcc tgatatggtt ccaccccagc gatggcgaga atactcaggg acgatgtttt
     2701 acggttttgc ccttcttgca tgttgcttct ccttaacacg cactatcgat ccccatgttc
     2761 gggggaaaaa tgaggccgct ataaatagca gatgcttttc cggatagcaa ttatctaaac
     2821 gtaacacttg acaactgtgt gaggaaaagc gtatttgcgc acgcgaccag aatgtaaatt
     2881 aaccagttac ttactttact acaatgtaac cggcagtgat tttttaagag ccatgccttc
     2941 agcagaaatt tccgctttgt aagccagaat ttctaccccc ctctgttgag cttctgacaa
     3001 tagttgcgcg tatttctcat cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg
     3061 cagcacggcg aaaaaaataa ccgcacgctg gccttcagcc gctacgctca tcaactcccg
     3121 aaggtgtttc tgacctcgtt cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc
     3181 taacgtaacc gatttcactt caatatagca gtctggacgc gaatccgcct gcaacataaa
     3241 gtcaatacgg ctgcgttcgg agccgtattt tacttcgctt ttcagcgagc tatagcctga
     3301 cagttctgaa attgattcat taaggatagc ctctttcgtc aacctgttag cccaaagcgt
     3361 attgacgcaa ataaatgcgc cgctctggct ttgagttaat tcccaggtgt gtgggtattt
     3421 ccgtttggtg ttgtctgaag tcgaatacca gacggtatcg ccaggcgttg cacaaccggt
     3481 catcgcaccc gtattcgggc agtgtagcgt taattcgcga ccatcgggtg tgatcacatc
     3541 ggctaaaaaa cgtttgtaac gctgaattag cgtcgcgcgc tgtagagggg gagaaaattc
     3601 cattgcgaca atccttgtta ttgcgttagc gcccagcgtt ttagcggcgt gtagcgtgtg
     3661 cgtccacggg caaacgagga ggcgtaaagg gtgaactccg tcaccgcata agaccagtta
     3721 aaacccggcg gcgggaatgt caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga
     3781 tgaaacggac gattgctttg aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc
     3841 gccagctgga ttaagccgcg tggaggctga cgcatcccta accacaccac acgcgaacgc
     3901 agccattgtc cggcgtcatc aagcgtgagt gtgaaaccag gttgacgaat ccgtccggct
     3961 aaaagagaaa gcgccttctc tttctctgcg ctcacttcgc ctaaaaatgc cagagtcagg
     4021 tgcaaattat cggcggcgac cggacgtccc gcctcaggtg ggaagtgtgt ggcgcgccaa
     4081 tggataatct gttcgcggat ttctgcaggt aagtcgatag caaagaacag acgttgcggt
     4141 tcagacatgt gaggcactcg gttataaatt accgcgatgc tacaatgtgg cgcgaagaat
     4201 gttaaccctc tggagcgttt tgtgtcgtcg ttgcccgttg ctgccgtctt acctgaatta
     4261 cttaccgctc tcgattgtgc gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa
     4321 tcaacctggc tgccgctgca actgctggcg catcccggca ttaacgggaa aattatcctg
     4381 ctggagccgc gtcgtctggc ggcgcgtaac gtcgcgcaac ggctggcgga gctgcttaac
     4441 gaaaagccag gcgataccgt tggctaccgg atgcgtgcgc aaaactgcgt cgggctgaat
     4501 acccgcctgg aagtggttac cgaaggcgtg ctgacgcgga tgatccagcg tgacccggaa
     4561 ctgagcggtg ttggattggt gatccttgat gagtttcatg agcgcagttt gcaggcggat
     4621 ttggcgttgg cgctgttact cgatgtgcaa caaggtctgc gtgatgacct taaactgctg
     4681 attatgtcgg ctacgctgga caatgaccgc ttgcagcaaa tgctgccgga agcgcccgtc
     4741 gtcatctcag aagggcgctc gtttccggtt gaacgccgtt atttaccgct gcccgcgcat
     4801 cagcgttttg acgaagccgt tgcggtagcc accgccgaaa tggtgcgtca ggaaagtgga
     4861 tcattactgt tatttttacc tggcgtcgga gaaattcagc gcgtgcagga acaactggct
     4921 tcgcgcatcg gcagtgatgt ggtgctctgc ccgctgtatg gcgtgttgtc actgaacgat
     4981 cagcgaaaag cgatcctccc ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat
     5041 attgctgaaa ccagtttaac catcgaaggt attcgtctgg tggtggattg tgcccaggag
     5101 cgtgtggcgc gctttgatcc gcgcacgggg cttacgcggt tgattactca acgcgttagt
     5161 caggcatcca tgacgcagcg tgccgggcgc gccgggcgtc tggagccggg tatcagcctg
     5221 catttaatcg ccaaagaaca agcagaacgc gccgcggcgc aaagtgagcc ggagatctta
     5281 caaagcgatc tttccggttt gctgatggaa ttactgcaat ggggatgcag cgatccggcg
     5341 cagatgagct ggctggatca accgccaacg gtgaatctac tggccgcgaa acgcctgtta
     5401 caaatgctgg gggcgctgga gggtgaacgg cttagtgcgc aagggcaaaa aatggcagcg
     5461 ctgggtaacg atccgcgttt agcggcaatg ctggttaacg caaagagcga tgacgaagct
     5521 gctaccgcgg caaaaattgc cgccattctc gaagagccgc cacggatggg caatagtgac
     5581 ctgggcgtgg cgttttcgcg caatcaacca gcctggcagc aacgtagtca gcaactgtta
     5641 aaacgcttaa acgtacgcgg cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc
     5701 ggggcgtttg ccgatcgcat tgctcatcgc cgtgggcaag atggacgcta tcaactggca
     5761 aacagcatgg gagcgatgct cgatgccgac gacgcgctaa gccgccacga atggttgatc
     5821 gcaccgttat tattgcaggg cagcgcctcg ccggatgcgc ggattttact ggcgctaccg
     5881 gtcgatattg atgaattagt acaacgctgt ccgcagctgg tgcagcaatc tgacaccgtg
     5941 gaatgggatg acgcgcaagg tacgctgaaa gcctggcgtc ggctacaaat cggtcagttg
     6001 acggtgaaag tgcagccgct ggcgaaaccg tcagaagacg agttgcatca ggcgatgctt
     6061 aatggcatcc gtgataaagg tttaagcgtg ctcaactgga cggcggaagc ggaacagcta
     6121 cgcttgcgtt tgttatgcgc cgcaaagtgg ttgccggaat atgactggcc agcggttgat
     6181 gatgaaagtt tgttggcgac gctggaaacg tggctgctgc cacatatgac cggcgtacat
     6241 tcactacgcg gcctgaaatc actcgacatt tatcaggcac tacgcggatt acttgattgg
     6301 ggaatgcagc aacgtctgga tagtgaattg cctgcgcatt acactgtgcc gacgggaagc
     6361 cggatcgcca ttcgttatca tgaagataac ccgcccgcgc tggcggtgag aatgcaggag
     6421 atgtttggtg aggccactaa tccgacgatc gcccaggggc gcgtgccgct ggtgctgaag
     6481 ttgctttccc ctgcccaaag gccgctgcaa atcacgcgtg atttgggggc cttctggaaa
     6541 ggggcgtatc gtgaggtgca aaaggagatg aaagggcgtt atcccaaaca cgtctggccg
     6601 gacgacccgg caaacacggc accgacgcga cggacgaaaa agtattcgta aggttggttt
     6661 tctccctctc cctgtgggag agggccgggg tgagggcatc agcgcgcacg ttcaccctca
     6721 ccctaaccct ctccctcaag ggagagggga ccgatcgagc gcaaattttg agagatatct
     6781 tcttctgtct tgtaacagaa gaacagaaaa tcgggctttt gcgcctgaat attgcggaga
     6841 aaaagcatgg ccgggaatga ccgcgagcca attggacgca aagggaaact gacgcgtccg
     6901 gtcaaacaaa aggtaagccg tcgtcgttac gaagatgacg atgattacga cgattatgat
     6961 gactatgagg atgaagaacc gatgccgcgc aaaggtaagg gcaaaggcaa agggcgtaag
     7021 cctcgtggca aacgcggctg gctatggcta ctgctaaaac tggctatcgt ttttgccgtg
     7081 ctgatcgcca tttacggcgt ttatctcgat caaaaaattc gtagccgtat tgatggcaag
     7141 gtctggcaac tgcccgcggc agtttatggc cgaatggtca accttgaacc agatatgacc
     7201 atcagcaaga acgaaatggt gaagctgctg gaggcaacac agtatcgtca ggtgtcgaaa
     7261 atgacccgtc ctggcgaatt taccgtgcag gccaacagca ttgagatgat ccgccgtccg
     7321 tttgatttcc cggacagtaa agaagggcag gtgcgtgcgc gtctgacctt tgatggtgat
     7381 catctggcga ccatcgtcaa tatggagaac aaccgtcagt tcggtttctt ccgtcttgat
     7441 ccgcgtctga tcaccatgat ctcctcgcca aacggtgagc agcgtttgtt tgtgccgcgc
     7501 agtggtttcc ctgatttgct ggtggatact ttgctggcga cagaagaccg tcatttttac
     7561 gagcatgatg gaatcagtct ctactccatt gggcgtgcgg tgctggcaaa cctgaccgca
     7621 ggtcgcacgg tgcagggtgc gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc
     7681 tccagcgagc gttcttactg gcgtaaagcg aacgaagctt acatggcgct gatcatggac
     7741 gcgcgttaca gcaaagaccg tattcttgag ctgtatatga acgaggtgta tctcggtcag
     7801 agcggcgaca acgaaatccg cggcttcccg ctggcaagct tgtattactt tggtcgcccg
     7861 gtagaagagc taagcctcga ccagcaggcg ctgttagtcg gtatggtgaa aggggcgtcc
     7921 atctacaacc cgtggcgtaa cccgaaactg gcgctggagc gacgtaatct ggtgctgcgt
     7981 ctgctgcaac agcaacagat tattgatcaa gaactctatg acatgttgag tgcccgtccg
     8041 ctgggggttc agccgcgtgg tggggtgatc tctcctcagc cagcctttat gcagctggtg
     8101 cgtcaggagt tgcaggcaaa actgggcgat aaggtaaaag atctctccgg cgtgaagatc
     8161 ttcactacct ttgactcggt ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt
     8221 ccggcactga agaaacagcg taagttgagc gatcttgaaa ctgcgattgt ggtcgtcgac
     8281 cgctttagtg gtgaagttcg tgcgatggtc ggaggttctg agccgcagtt tgcgggctac
     8341 aaccgtgcga tgcaggcgcg tcgttcgatt ggttcccttg caaaaccagc gacttatctg
     8401 acggctttaa gccagccgaa aatctatcgc ctgaacacgt ggattgccga tgcgccaatc
     8461 gcgctgcgtc agccgaatgg ccaggtctgg tcaccgcaga acgatgaccg tcgttatagc
     8521 gaaagcggca gagtgatgct ggtggatgcg ttgacacgtt cgatgaacgt gccgacggta
     8581 aatctgggga tggcgctggg gctgcctgcg gttacggaga cctggatcaa actgggcgta
     8641 ccgaaagatc agttgcatcc ggttccggca atgctgctgg gggcgttgaa cttaacgcca
     8701 atcgaagtgg cgcaggcatt ccagaccatc gccagcggtg gtaaccgtgc accgctttct
     8761 gcactgcgtt cggtaatcgc ggaagatggt aaggtgctgt atcagagctt cccgcaggca
     8821 gaacgcgctg ttccggcgca ggcggcgtat ctgacactat ggaccatgca gcaggtggta
     8881 caacgtggta cgggtcgtca gcttggggcg aaatacccga acctgcatct ggcagggaaa
     8941 acagggacta ccaacaataa cgtagatacc tggtttgcag gcattgacgg cagcacggtg
     9001 accatcacct gggtaggccg tgataacaac cagccgacca aactgtatgg agccagcggg
     9061 gcaatgtcga tttatcagcg ttatctggct aaccagacgc caacgccgct gaatcttgtt
     9121 ccgccagaag atattgcaga tatgggcgtg gactacgacg gcaactttgt ttgcagcggt
     9181 ggcatgcgtg tcttgccggt ctggaccagc gatccgcaat cgctgtgcca gcagagcgag
     9241 atgcagcagc agccgtcagg caatccgttt gatcagtctt ctcagccgca gcaacagccg
     9301 caacagcaac ctgctcagca agagcagaaa gacagcgacg gtgtagccgg ttggatcaag
     9361 gatatgtttg gtagtaatta acatctaagc gtgaaatacc ggatggcgag ttgccatccg
     9421 gtaaaataac atcccatcta agatattaac cctttctttt catctggttg tttattaacc
     9481 cttcaggaac gctcagattg cgtaccgctt gcgaacccgc cagcgtttcg aatattatct
     9541 tatctttata ataatcattc tcgtttacgt tattattcac tttacatcag agatatacca
     9601 atggcgcgtt ccaaaactgc tcagccaaaa cactcactgc gtaaaatcgc agttgtagta
     9661 gccacagcgg ttagcggcat gtctgtttat gcacaggcag cggttgaacc gaaagaagac
     9721 actatcaccg ttaccgctgc acctgcgccg caagaaagcg catgggggcc tgctgcaact
     9781 attgcggcgc ggcagtcagc taccggcact aaaaccgata cgccgattca aaaagtgcca
     9841 cagtctattt ctgttgtgac cgccgaagag atggcgctgc atcagccgaa gtcggtaaaa
     9901 gaagcgctta gctacacgcc gggtgtctct gttggtacgc gtggcgcatc caacacctat
     9961 gaccacctga tcattcgcgg ttttgcggca gaaggccaaa gccaaaataa ctatctgaat
    10021 ggcctgaagt tgcagggcaa cttctataac gatgcggtca ttgacccgta tatgctggaa
    10081 cgcgctgaaa ttatgcgtgg cccggtttcc gtgctttacg gtaaaagcag tcctggcggt
    10141 ctgttgaata tggtcagcaa gcgtccgacc accgaaccgc tgaaagaagt tcagtttaaa
    10201 gccggtactg acagcctgtt ccagactggt tttgacttta gcgatgcgtt ggatgatgac
    10261 ggtgtttact cttatcgcct gaccggtctt gcgcgttctg ccaatgccca gcagaaaggg
    10321 tcagaagagc agcgttatgc tattgcaccg gcgttcacct ggcgtccgga tgataaaacc
    10381 aatttcacct tcctttctta cttccagaac gagccggaaa ccggttatta cggctggttg
    10441 ccgaaagagg gaaccgttga gccgctgccg aacggtaagc gtctgccgac agactttaat
    10501 gaaggggcga agaacaacac ctattctcgt aatgagaaga tggtcggcta cagcttcgat
    10561 cacgaattta acgacacctt tactgtgcgt cagaacctgc gctttgctga aaacaaaacc
    10621 tcgcaaaaca gcgtttatgg ttacggcgtc tgctccgatc cggcgaatgc ttacagcaaa
    10681 cagtgtgcgg cattagcgcc agcggataaa ggccattatc tggcacgtaa atacgtcgtt
    10741 gatgatgaga agctgcaaaa cttctccgtt gatacccagt tgcagagcaa gtttgccact
    10801 ggcgatatcg accacaccct gctgaccggt gtcgacttta tgcgtatgcg taatgacatc
    10861 aacgcctggt ttggttacga cgactctgtg ccactgctca atctgtacaa tccggtgaat
    10921 cccgatttcg acttcaatgc caaagatccg gcaaactccg gcccttaccg cattctgaat
    10981 aaacagaaac aaacgggcgt ttatgttcag gatcaggcgc agtgggataa agtgctggtc
    11041 accctaggcg gtcgttatga ctgggcagat caagaatctc ttaaccgcgt tgctgggacg
    11101 accgataaac gtgatgacaa acagtttacc tggcgtggtg gtgttaacta cctgtttgat
    11161 aatggtgtaa caccttactt cagctatagc gaatcgtttg aaccttcttc gcaagttggg
    11221 aaggatggta atattttcgc accgtctaaa ggtaagcagt atgaagtcgg cgtgaaatat
    11281 gtaccggaag atcgtccgat tgtagttact ggtgccgtgt ataatctcac taaaaccaac
    11341 aacctgatgg cggaccctga gggttccttc ttctcggttg aaggtggcga gatccgcgca
    11401 cgtggcgtag aaatcgaagc gaaagcggcg ctgtcggcga gtgttaacgt agtcggttct
    11461 tatacttaca ccgatgcgga atacaccacc gatactacct ataaaggcaa tacgcctgca
    11521 caggtgccaa aacacatggc ttcgctgtgg gctgactata ccttctttga cggtccgctt
    11581 tcaggtctga cgctgggcac cggtggtcgt tatactggct ccagctatgg tgatccggct
    11641 aactccttta aagtgggaag ttatacggtc gtggatgcgt tagtgcgtta tgatctggcg
    11701 cgagtcggca tggcgggctc caacgtggcg ctgcatgtta acaacctgtt cgatcgtgaa
    11761 tacgtcgcca gctgctttaa cacttatggc tgcttctggg gcgcagaacg tcaggtcgtt
    11821 gcaaccgcaa cctttcgttt ctaatttctc ttttggggca cggatttccg tgcccatttc
    11881 acaagttggc tgttatgcag gaatacacga atcattccga taccactttt gcactgcgta
    11941 atatctcctt tcgtgtgccc gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg
    12001 ccgggaaagt gaccggtctg attggtcaca acggttctgg taaatccact ctgctcaaaa
    12061 tgcttggtcg tcatcagcct ccgtcagaag gggagattct tcttgatgcc cagccgctgg
    12121 aaagctggag cagcaaagcg tttgcccgca aagtggctta tttgccgcag cagcttcctc
    12181 cggcagaagg gatgaccgtg cgtgaactgg tggcgattgg tcgttacccg tggcatggcg
    12241 cgctggggcg ctttggtgcg gcagatcgcg aaaaggtcga ggaagctatc tcgctggttg
    12301 gcttaaaacc gctggcgcat cggctggtcg atagtctctc tggcggcgaa cgtcagcggg
    12361 cgtggatcgc catgctggtg gcgcaggata gccgttgtct gttgctcgac gaaccgactt
    12421 cagcgctgga tatcgcccac caggttgatg tgctggcgct ggtgcaccgt ttaagtcagg
    12481 aacgtggcct gacggtcatt gccgtgttgc acgatatcaa tatggcggca cgctactgtg
    12541 attatctggt cgccctgcgc agtggtgaaa tgattgctca gggaacgcct gcggaaatta
    12601 tgcgcggcga aaccctcgaa atgatttatg gcatcccgat gggtattttg ccgcatccgg
    12661 cgggtgctgc acctgtgagt tttgtttatt gatgagcggc ttacctctta tttcgcgccg
    12721 tcgactgtta acggcgatgg cgctttctcc gttgttatgg cagatgaata ccgcccacgc
    12781 ggcggctatt gaccccaatc gtattgtggc gctggagtgg ttgccggtgg aattactgct
    12841 ggcgctcggc atcgtgcctt acggcgtggc ggataccatc aactatcgcc tgtgggtcag
    12901 cgaaccacca ttgccggact cagtgattga cgtcggtttg cgtacagaac ctaaccttga
    12961 actgatgacc gaaatgaaac cgtcgtttat ggtctggtcg gcaggatatg gcccttcatc
    13021 agaaatgctg gcacgtattg cgccgggtcg cggatttaac ttcagtgacg gcaaacatcc
    13081 gttggcgatg gcgcgtaaat cgctgacgga aatggcagat ttacttaacc tgcaaagcgc
    13141 agcggaaacg catttagcgc aatacgaaga ctttatccgc agcatgaaac cccgctttgt
    13201 gaagcgtggt gcacgcccgt tactgctgac gacgcttatc gatccgcgcc atatgctggt
    13261 cttcggtcca aacagcttgt tccaggaaat tctcgatgag tacggcatcc caaatgcctg
    13321 gcaaggggaa accaacttct ggggcagtac cgccgtcagt atcgatcgtc tggcagcgta
    13381 taaagacgtt gatgtgctct gttttgatca cgacaacagc aaagacatgg atgcgctaat
    13441 ggcaaccccg ctgtggcagg ccatgccgtt tgtccgtaca gggcgctttc agcgcgtacc
    13501 cgcagtctgg ttttatggtg caacgctttc ggcaatgcac tttgtgcgtg ttctggataa
    13561 cgccatcgga ggtaaagcgt gagtaaacga attgcgcttt tcccggcgtt attgctggcg
    13621 ctgttagtga ttgtcgctac ggcgctcacc tggatgaact tctcacaggc gctgccgcgc
    13681 agtcagtggg cgcaggccgc ctggtcgccg gatattgacg tcatcgagca gatgattttt
    13741 cactacagct tgttgccgcg tctggcgatt tcgctgctgg tgggcgcggg cctggggctg
    13801 gtgggcgtgc tgtttcagca agtactgcgt aacccgctgg cggaaccgac gacgttgggc
    13861 gttgcaacag gcgcgcaact ggggattacc gtcaccacgc tctgggcgat ccctagcgcg
    13921 atggcgagcc agtttgctgc gctggcaggg gcttgcgttg ttggcttaat tgtctttggc
    13981 gtcgcgtggg ggaaacggct gtcgccggta acgctgattt tcgcggggtt ggtagtgagc
    14041 ctttattgcg gcgcaatcaa tcagttactg gttatcttcc atcatgacca gttgcaaagc
    14101 atgtttctgt ggagcactgg aacgctgacg caaaccgact ggggcggtgt tgagcgttta
    14161 tggccgcagt tgctgggcgg tgtgatgctg acgttgctgc tacttcgtcc gttaaccctg
    14221 atggggcttg atgatggcgt ggcgcgcaat ctcgggctgg ccttgtcgct tgcgcgtctg
    14281 gcggcgctgt cgctggcgat tgtcatcagt gcgctgctgg tgaacgctgt ggggattatc
    14341 ggctttatcg ggttgttcgc gccgctgctg gcaaaaatgc tgggggcgcg gcgtctgctg
    14401 ccacgactga tgctggcgtc gttgattggt gcgctgatcc tctggctttc cgatcaaatc
    14461 atcctctggc tgactcgcgt gtggatggaa gtgtccaccg gttcggtcac tgcgttgatc
    14521 ggtgcgccgc tgctactgtg gctgctgccg cgtttacgca gcattagcgc gccggatatg
    14581 aaggttaacg atcgtgtcgc gactgaacgc caacatgtgc tggcgtttgc cctcgcgggc
    14641 ggcgtgctgt tgttgatggc tgtggtggtg gcgctgtcgt ttggtcgtga tgcgcacggc
    14701 tggacgtggg cgagcggggc gttgctcgag gatttaatgc cctggcgctg gccgcgaatt
    14761 atggcggcgc tgtttgcggg cgtcatgctg gcggtagcgg gctgtattat tcagcgactg
    14821 accggcaacc cgatggcaag cccggaagtg ctggggatta gctccggcgc ggcgtttggt
    14881 gtggtgttaa tgctgtttct ggtaccgggt aatgcctttg gctggttgtt acccgcaggc
    14941 agtctcgggg cggcggtgac gctgttgatc attatgatcg ccgccggacg cggtggattt
    15001 tccccacacc gcatgttact ggcggggatg gcgttaagta ccgcgttcac catgcttttg
    15061 atgatgttgc aggcaagtgg tgacccgcga atggcgcaag tgctgacctg gatttccggt
    15121 tcgacctaca acgcgaccga tgctcaggtc tggcgcaccg gaattgtgat ggtgattttg
    15181 ctggcgatta ctccgctgtg ccgccgctgg ctgaccattt taccgctggg tggtgatacc
    15241 gcgcgggcgg tagggatggc gctgacgccg acgcgaattg cgctgctgct gttagcggct
    15301 tgcctgacgg cgaccgcgac gatgactatt ggaccgttga gttttgttgg tttaatggca
    15361 ccgcatattg cgcggatgat gggctttcga cggacgatgc cacacatcgt aatttcggcg
    15421 ctggtgggtg gtttactgct ggtgttcgct gactggtgtg ggcggatggt gctgtttcca
    15481 ttccagatcc cggcggggct gctgtcgacc tttatcggtg cgccatattt tatctatttg
    15541 ctgagaaagc agagccgtta atagaatgcc tcgcccggtg ggcgaggcaa aattatcaca
    15601 acttcgcgaa cacccgacgt gcagcatcga tggtgttatt gatatcttcc atgctgtgcg
    15661 ccacagacat aaagcccgct tcaaacgctg acggtgccag gtaaacacct tcatccagca
    15721 tcatatggaa gaaacgctta aaacgctcga cgtcacaggc catcacatcc tgatagcacg
    15781 tcacggactc ggcgtcggta aagaagatac cgaacatacc accgacgtgg ttaacgacca
    15841 gcggaattcc cgcttcttct gccgcttcca gcagaccttc tgccagacgt gttgtcagct
    15901 catccagcgt ttcgtgaatg cccggctgcg cgacttcatt cagacaggcg aaacccgctg
    15961 ccatcgcaat cgggttaccg gaaagcgtac ccgcctgata gaccggacct gtcggggcca
    16021 gcgcatccat tacatcacga cgaccaccga atgcgcctac cggcattcca ccgccgatga
    16081 tttttccgag gcaggttaaa tctggcacta cggcgaaata atcctgtgcg ccagctagcg
    16141 ctacgcggaa accggtcatc acttcatcga tgatcagcaa cgcgccaaat tcgtcgcaca
    16201 gcgcacgcag acctggcagg aactctggca gcggcggaac acagttcata ttgcctgcca
    16261 ccggctcgac gataatacag gcaatctctt gcgggtattg ctcaaacgcg gcgcgtacag
    16321 aggccagatc gttataagta caggttaagg tatgtttggc gaaatctgcc ggaacgcccg
    16381 gcgagtttgg ctggcctaac gtgagtgcgc cagaaccggc tttcaccagc aggcagtcag
    16441 cgtgaccgtg gtaacaacct tcaaatttaa tgattttgtc gcgaccggta aaaccacggg
    16501 ccaggcggat ggcgctcatc gtcgcctcgg tgccggagtt caccatgcgc accatatcca
    16561 tggtcgggac cagttcggtc accagttgcg ccattttcac ttccatttcg gtcggtgcac
    16621 caaagcttaa accacgctcg gcggcttcaa tcacggcatt gcggatcgcc ggatggttat
    16681 ggcccagcac catcggcccc caggaaccga cataatcgat ataggctttg ccatcaacat
    16741 cgtacagata agcgccgtcc gctttttcga taaacagtgg agtgccgccc acgccagtaa
    16801 aggcgcgaac aggggagttc acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat
    16861 tttcagactt actcatggag ggttcctgat tcgtagaaaa agtgaatggc tgctattcca
    16921 tgttattcat aacaagttaa atactcgtca aacatcaggc tgcttgtact ggtgcaatcc
    16981 tgatttctta gagtataaaa gttttgtgca tttgaaacat tacgctttgc aaaggatttt
    17041 catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat gaccaataag tgatcattgg
    17101 atgaaaactg atactccctc tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa
    17161 ctgattcgcc aacttcttga gcgcgataaa accccgttag ccattttgtt tatggcggca
    17221 gtcgtcggca cgcttgtcgg gctggctgcg gttgcttttg acaaaggtgt cgcttggttg
    17281 cagaaccaac gtatgggggc gctggtacat actgctgata attatccgct actgttaacc
    17341 gtcgcttttc tctgttcggc ggtgctggcg atgttcggct actttttggt gcgcaaatac
    17401 gcgcccgaag cagggggatc ggggatcccg gaaattgaag gggcgctgga agatcaacgt
    17461 cctgttcgct ggtggcgtgt attgccggtg aaattctttg gcgggctggg gacactcggc
    17521 ggaggcatgg ggctagggcg cgaaggacca accgtgcaga tcggcggcaa tatcagtcgc
    17581 atggtgcttg atattttccg cctgaaaggt gatgaagctc gccatacgct gctggcaacc
    17641 ggtgctgctg cggggctggc tgcggccttt aacgcgccgc tggcgggtat tttgtttatt
    17701 atcgaagaga tgcgtccgca gtttcgctat acgttaattt cgattaaagc ggtatttatt
    17761 ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc atgaagttgc gttgattgac
    17821 gtcggtaaac tttctgatgc gccgcttaat acgctgtggc tttatctgat cctcggtatt
    17881 atttttggca ttttcggccc tatttttaat aaatgggtgc tggggatgca ggatttgctg
    17941 caccgtgtgc acggcggcaa tattaccaaa tgggtactga tggggggtgc gattggcggt
    18001 ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg gcggcggttt taacctgatt
    18061 cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg tgtttatctt cgtcgctcgg
    18121 gtcattacca ccttactctg cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg
    18181 ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg ttgccgttga gctgtttccg
    18241 caatatcacc tggaggcggg gacgtttgct attgccggaa tgggggcatt actggcggca
    18301 tctattcgcg cgccgttaac ggggatcatt ctggttctgg agatgaccga taactaccag
    18361 ctcattttgc caatgattat taccggtctt ggcgcaacac tattagcgca atttaccggc
    18421 gggaaacctc tatactcggc gattcttgcg cgcacgctgg caaaacagga agctgagcaa
    18481 ctggcgcgaa gcaaggccgc atcagccagc gagaatactt gaacgaaata ccagggtatt
    18541 agataatggc gattattatt gggttagaat ttgcccgatt gccgatgtcg tttggagcaa
    18601 aatatgagtg atgacgtagc actgccgctg gagtttaccg acgcagcagc caacaaagtt
    18661 aaaagcctga tcgctgacga agataacccg aatctgaaat tacgcgtgta tatcaccggt
    18721 ggcggttgca gcggcttcca gtatggtttc acctttgatg atcaggtgaa cgaaggcgat
    18781 atgaccatcg aaaaacaggg cgttggcctg gtggttgatc cgatgagcct gcaatatctg
    18841 gtcggcggtt ccgttgacta caccgaagga ctggaaggtt ctcgtttcat cgtgaccaac
    18901 ccgaacgcga aaagcacctg cggttgcggt tcttccttta gcatctaatc tgttgttcat
    18961 gtgccggata aactatccgg cacatcttct ctcttcaacg cccattctca tccagcgcaa
    19021 acgtcggtag cttaagatgc caacgaatag ccgccagccg aatcaatagc gtcacgacca
    19081 tgcccatcat actggctgtt tccagtggta cggaaaatgt gtaataagcc gtagcgtgga
    19141 caataccgcc gataatacag gcagttgcgt agatttctgt acgtaaaatc atggggattt
    19201 cgcggaccag tacatcacga atgatctcgc cgccaacgcc agtaatgacg cccatacaaa
    19261 ccgcgattaa cggaccggct tccgcattaa aggctttatt cacgccaatg ccgacaaaca
    19321 ccgccagacc aacggcgtcc agcaccggca acatccattt tggtaagcgt cttggctggc
    19381 gcaccagcac gatggtcagc atgctggtga ccatggcaac gaccagatcg gtgggatctt
    19441 tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg aattgttccg tcgcctactg
    19501 cggtaaccac gcccagtacc agaacaccaa aagggtccat acgcaatttt ccggctaaca
    19561 aaacgccgta gatggcaaat accgctgtgc cgactatatc cagccaatag acgagcattg
    19621 ttcaatcccc actgagcacc tgaaaaggtc aggcactaat ctacctgtga aagcgcatta
    19681 cagagctgtt gtgctgcgag gataatacgt gggcttgcac gttcaaacca gtcactcgtg
    19741 agaggaataa cgggaatttt gagctgttca ccccagtatt gtttgatttt aggaatttgg
    19801 tccggtccgc ctgtaatgac aatagcctgt ggcgagcgtg ctaacacctg ttcgcggcta
    19861 acttgcggcc agggaacccg gctgtcttta aagatgtttt ctccgccaca aacttcgagt
    19921 acctggttct gaatcgactc ttttccactg gtaaatggcg gattaatgcc gaattgcaga
    19981 aaaacacgtt ttttaggttt atcagcatat tgcgctttca attgcacgta ctgatccagc
    20041 agggattgcg cggcttgttc ggccttgtct ggttgcggac tccagggggc cagttgacgt
    20101 aacgcattgg caatttgttc aatgcttgtc gcatcgaccc acatcacttt tattcccagc
    20161 gaagccagct ggtcaacctg ccgctcggca ttacctccac gccaggcaat caccagatcg
    20221 ggtttcagcg cgacaatgcg ttccagattc atcccctgcc aggtggaaac ctgctcaatc
    20281 ttttgcgctt gtggaggata gtcggaatag ctgctgaccc caaccggcgt gatcccggcg
    20341 gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc
    20401 cacagtggcg cgagaaaaga cagggcgacc agcgccctga acagtgattt agccatgtgc
    20461 cagtttctgc accagtgact caaccatcag gctggactgt ttagcggcaa cagccaggaa
    20521 ctcatcgaag ctaagatgag actgttgatc ggccacgtcg gagatggcgc gtacgacaac
    20581 aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca tctctacagc
    20641 aatggcctgt gggaagttgt ggcggatttt cgccagacca acagaaccgt tgatgaaagc
    20701 gtcgccgcta acaatcaggc cacgtacagc gttaagattc agttcggcaa tgcaggcctc
    20761 agcggcagcg atcagtttat cgtcagcttt aaagcctgcc ggacagcctg gtaactgacc
    20821 gtattcataa ccaaatgccg tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac
    20881 gatatcgccc actttcagcg ttggtgccag gccaccggca gaaccggtgt taataatcac
    20941 atctggcttg cagtgttcca acagcagagt ggcacccagc gccgcagcga ctttaccgat
    21001 gcccgatttc agaagcgcaa cctcggttcc attcagttgg ccggtataga tttcgcaacc
    21061 gccgagactg atagtttgac ggttttcgat tttgtcacgc agcagcgtaa cttcttcttc
    21121 cattgcacca atgatgccga ttttcataga tttactcgcg ataagcccga tttgaaggca
    21181 tagtttacca tgcgcttacg gggaagcgta tttctcacgc gggagaggac atggcacaga
    21241 ttgatttccg aaaaaaaata aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa
    21301 ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg
    21361 caattcgtcg tctgcaacaa aagacccagg tttttccact ggagcgcaat gccgccgtgc
    21421 gcacgcgtct tacccactcg atggaagtcc agcaggtggg acgctacatc gccaaagaaa
    21481 ttttaagccg tctgaaagag cttaaattgc tggaagcata cggcctggat gaactgaccg
    21541 gcccctttga aagcattgtt gagatgtcat gcctgatgca cgatatcggc aatccgccgt
    21601 ttggtcattt tggcgaagca gcgataaatg actggtttcg ccaacgtttg cacccggaag
    21661 atgccgaaag ccagcctctg actgacgatc gctgcagcgt ggcggcacta cgtttacggg
    21721 acggggaaga accgcttaac gagctgcggc gcaagattcg tcaggactta tgtcattttg
    21781 aggggaatgc acaaggcatt cgtctggtgc atacattgat gcggatgaat ctcacttggg
    21841 cacaggttgg cggtatttta aaatataccc gtccggcgtg gtggcgtggc gaaacgcctg
    21901 agacacatca ctatttaatg aaaaagccgg gttattatct ttctgaagaa gcctatattg
    21961 cccgcttgcg taaagaactt aatttggcgc tttacagtcg ttttccatta acgtggatta
    22021 tggaagctgc cgacgacatc tcctattgtg tggcagacct tgaagatgcg gtagagaaaa
    22081 gaatatttac cgttgagcag ctttatcatc atttgcacga agcgtggggc cagcatgaga
    22141 aaggttcgct cttttcgctg gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt
    22201 taagccgcag tacggaagat cagtttttta tgtatttacg ggtaaacacc ctaaataaac
    22261 tggtacccta cgcggcacaa cgatttattg ataatctgcc tgcgattttc gccggaacgt
    22321 ttaatcatgc attattggaa gatgccagcg aatgcagcga tcttcttaag ctatataaaa
    22381 atgtcgctgt aaaacatgtg tttagccatc cggatgtcga gcagcttgaa ttgcagggct
    22441 atcgggtcat tagcggatta ttagagattt atcgtccttt attaagcctg tcgttatcag
    22501 actttactga actggtagaa aaagaacggg tgaaacgttt ccctattgag tcgcgcttat
    22561 tccacaaact ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt aaattaccgt
    22621 cagattctcc tgagtttccg ctatgggaat attattaccg ttgccgcctg ctgcaggatt
    22681 atatcagcgg tatgaccgac ctctatgcgt gggatgaata ccgacgtctg atggccgtag
    22741 aacaataacc aggcttttgt aaagacgaac aataaatttt taccttttgc agaaacttta
    22801 gttcggaact tcgggctata aaacgaatct gaagaacaca gcaattttgc gttatctgtt
    22861 aatcgagact gaaatacatg aaaaaaacca cattagcact gagtgcactg gctctgagtt
    22921 taggtttggc gttatctccg ctctctgcaa cggcggctga gacttcttca gcaacgacag
    22981 cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca
    23041 ttaacgtaga aggtagcaca accgttaata cgccgcgtat gccgcgtaat ttccagcagt
    23101 tcttcggtga tgattctccg ttctgccagg aaggttctcc gttccagagc tctccgttct
    23161 gccagggggg ccagggcggt aatggcggcg gccagcaaca gaaattcatg gcgctgggtt
    23221 ccggcgtcat cattgatgcc gataaaggct atgtcgtcac caacaaccac gttgttgata
    23281 acgcgacggt gattaaagtc cagctgagcg atggtcgtaa gttcgacgcg aagatggttg
    23341 gcaaagatcc gcgctctgat atcgcgctga tccaaatcca gaacccgaaa aaactgaccg
    23401 caattaagat ggcggattct gatgcactgc gcgtgggtga ttacaccgta gcgattggta
    23461 acccgtttgg tctgggcgag acggtaactt ccgggattgt ctctgcgctg gggcgtagcg
    23521 gcctgaatgc tgaaaactac gaaaacttca tccagaccga tgcagcgatc aaccgtggta
    23581 actccggtgg tgcgctggtt aacctgaacg gcgaactgat cggtatcaac accgcgatcc
    23641 tcgcaccgga cggcggcaac atcggtatcg gttttgctat cccgagtaac atggtgaaaa
    23701 acctgacctc gcagatggtg gaatacggcc aggtgaaacg cggtgagctg ggtattatgg
    23761 ggactgagct gaattccgaa ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt
    23821 tcgtaagcca ggttctgcct aattcctccg ctgcaaaagc gggcattaaa gcgggtgatg
    23881 tgatcacctc actgaacggt aagccgatca gcagctttgc cgcactgcgt gctcaggtgg
    23941 gtactatgcc ggtgggcagc aaactgaccc tgggcttact gcgcgacggt aagcaggtta
    24001 acgtgaacct ggaactgcag cagagcagcc agaatcaggt tgattccagc tccatcttca
    24061 acggcattga aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca
    24121 acgtgaagac gggcactccg gctgcgcaga tcggcctgaa gaaaggtgat gtgattattg
    24181 gtgcgaacca gcaggcagtg aaaaacatcg ctgaactgcg taaagttctc gacagcaaac
    24241 cgtctgtgct ggcactgaac attcagcgcg gcgacagcac catctatctg ttaatgcagt
    24301 aatctccctc aaccccttcc tgaatacggg aaggggttct ccttacaatc tgtgaacttc
    24361 gccacaactc catacatctt catcatcctt tgggcatttg cacaatgccg tacgttacgt
    24421 acttccttat gttaagccgt gcataacgga ggacttatgg ctggctggca tcttgatacc
    24481 aaaatggcgc aggatatcgt ggcacgtacc atgcgcatca tcgataccaa tatcaacgta
    24541 atggatgccc gcgggcgaat tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac
    24601 gaaggtgcat tgctggtact ttcacaggga cgagtcgtcg atatcgatga cgcggtagca
    24661 cgtcatctgc acggtgtgcg gcaggggatt aatctaccgt tacggctgga aggtgaaatt
    24721 gtcggcgtaa ttggcctgac aggtgaacca gagaatctgc gtaaatatgg cgaactggtc
    24781 tgcatgacgg ctgaaatgat gctggaacag tcgcggttga tgcacttgtt ggcgcaggat
    24841 agccgtttgc gggaagaact ggtgatgaac ctgattcagg cagaggagaa tactcccgca
    24901 cttctgaatg ggcgcaacgg ctggggatcg atctcaatca accgcgagtg gtggctattg
    24961 ttgaggtcga cagcggtcag cttggcgtgg acagcgcaat ggcggagtta caacaactgc
    25021 aaaacgcgct gactacgccc gagcgtaata atctggtggc gattgtctcg ctaaccgaaa
    25081 tggtggtgtt gaaaccggcg ttgaactctt ttgggcgctg ggatgcagaa gatcatcgta
    25141 agcgagttga acaactgatt acccgcatga aagagtacgg ccagctgcgt tttcgcgttt
    25201 cactgggcaa ctattttacc ggtcctggca gtattgcccg atcctatcgt acggcgaaaa
    25261 cgacgatggt ggtgggtaaa cagcggatgc cagaaagtcg ctgctatttt tatcaggatc
    25321 tgatgttacc tgtgttactc gacagtttgc gtggcgactg gcaggccaac gaactggcgc
    25381 gaccgctggc gcggctgaaa gcgatggaca ataacggctt gctgcgacga acgctggcgg
    25441 cgtggtttcg ccacaatgtg caaccgctgg caacgtcaaa ggcgttgttt attcatcgta
    25501 ataccctgga gtatcggctt aatcgtatat cggaactgac cgggcttgat ttgggcaatt
    25561 ttgatgacag gttgctgctg tatgtggcgt tacaactgga tgaagagcgg tagattatgc
    25621 gttaaggttg aggcggcgct ggctcattcc cctcagcccg aaggggagag gggaatgccc
    25681 gagttaaccg tcaattcggg attatttacg cgttagtttt tccagatcgg cttcaatctc
    25741 gctgatctta ttggttacga ctgactccag gtgacgtaag tcgtcgagga tcttacgctt
    25801 aagatcaact tcgctgcggt cacgctggca gatttgatca agctcatcaa tgatataccg
    25861 tagattcggg ctgatttcct ggacttcttt ataaccctga cccacaccat cagcgacgac
    25921 cgttttacgc tgacgcggat atttaaactt aacgctcttg gcgaaaaact cgcctttgtc
    25981 tttctggaaa tagattttca gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc
    26041 aatttcttca ggattggtaa tacccagact tttcagattg tcgtacatag cgttacctca
    26101 aaatgagtca gtaaattgtg cttattttag catttggctt ggcccgcccg gctcgactgt
    26161 gatcgcagaa agcctttttc ttccagactt ctatcatggc gtaaaaatca aaaaattacc
    26221 tgctttattg tggcgatatt ccatgggatg gattagcaca tttatcttcg ttttgcgaat
    26281 tacatgacag tccgaaacaa gatgaaaaag tatttaaaaa agggggagga agaatgagta
    26341 ggacgaatta ttctgagcca gaaagcatac gggcagcatc atgctgcccg tatatgtact
    26401 cttaatcgat ggtacgcagc agttcgttaa tgccgacttt gccgcgagtt ttcgcgtcaa
    26461 ctttcttaac gataaccgca cagtagaggc tgtatttgcc atcttttgac ggcagattac
    26521 ctgaaacaac cacagacccc gccggaacgc gaccgtagtg gatttcgccg gtttcacggt
    26581 cgtaaatacg ggtgctctga ccaatgtata cgcccataga gatgacggaa ccttcttcga
    26641 caatcacccc ttcaaccact tcagagcgtg cgccaatgaa gcagttatct tcaataatgg
    26701 tcgggttagc ctgcagcggc tccagcacgc cgccgatgcc cacgccaccg gaaaggtgga
    26761 cgtttttacc aatctgcgca caagaaccga cggtcgccca ggtatcaacc atggtgcctt
    26821 catcaacata tgcgccgatg ttaacgtaag acggcatcag cacggtgtta cgggcaataa
    26881 acgcaccctg acgtaccgcc gctggtggca caacgcggaa gccttctttc tggaaacgtg
    26941 cttcgtcgta gtcggcgaat ttcatcggca ctttgtcgaa gtagcggctt tctgcccctt
    27001 cgatcacctg attatcatta atacggaaag agagcagcac cgcttttttc aaccactgat
    27061 gcgtcaccca ctgaccgtca attttttccg ctacacgcag tgcgccggaa tccagcaggg
    27121 cgatcacctg atttaccgct tcgcgggtaa cggtgtctgc attggctggc gtgatctcgg
    27181 cacggcgttc aaaagcggtt tcaataatgt tctgtaactg ctgcattgtt aaactctttt
    27241 catatcagta aacacatcac cctttatcgt ttggattgag ggcctctgtc aaccgctgat
    27301 gcacttcctg ctgcaactcg ttattaagcg cacgccggtc agcggtggca attatgaata
    27361 aatcttctac tcgctcgcca atggttgtaa ttcgggcacc atgaagcgaa attcccagat
    27421 cggcaaaaat tttcccgact cgcgccagca gtccaggttg gtcgagggcg atcagttcga
    27481 ggaacgattt gcggtcggta tgggtcggca aaaacgttac ttcggtttca acagtaaaat
    27541 ggcgtaattt ggcgggttgg cgacggggct gcggtggctg ccagctactt tgcgtcagca
    27601 cctgctccag accaaaccga ataacctcat ggcgatctgc ggacagcggg ctgccatcgg
    27661 gttccagcac gataaaggta tccatcgcca taccgtcgcg agtggtgaaa atttgtgcgt
    27721 cgtgaacact taaattgcgg cggtctaatt cggcacagac ggcggcaaac agataggggc
    27781 ggtccgggct ccagataaaa atctcggtgc ctccacgcgt agcctgcggg ctaagcaata
    27841 ccagcggttt gcttaaatca tgctgtaata aatggcgggc atgccaggcc agttgatttg
    27901 ggctatggcg gacaaaatag ttagcacgac agcgtgacca aatttggtgc agcgcctctt
    27961 cgtcgatgtt atccatgcgc agtagtgcca gtgcctggag ttggtgatgg cgaacccgtt
    28021 cgcgcatatc cggcgtgttt tgcattccgc gtcgtagctg cttttcggtg gcaaaataga
    28081 gttcgcgcaa cagactttgc ttccagctat tccacagtgt ttcgttggtg gcgcaaatgt
    28141 cagccactgt caggcatacc agatagcgca gacgattttc cgtttgaact tcttcggcaa
    28201 actgcttgat gacttcaggg tcctgaatat cgcggcgttg tgctgtcacc gacatcagca
    28261 agtgctggtg aaccagccag gcaaccagct gtgtttcgcg tgagttcagc ccgtggagtt
    28321 ctgcaaaatg cactacatcc tgagcaccga gaatggagtg gtcgccgccg cgtcctttgg
    28381 cgatatcgtg aaacagcgcg gcgatgaaaa tcagctcagt tgacggcagg cgcggccaga
    28441 cgtccacaca caacggatgg cgctggcgcg tttcttcact ggcaaaactc tccagtttca
    28501 gcatcacgcg gatagtatgt tcatccaccg tgtaggcgtg gaacagatca aactgcatct
    28561 gcccgacgat atgcgaccat tgcggcatat acgcgccgag cacgctatgg cgatgcattg
    28621 gcaatagtcc gcgccgcacc gctccggggt gacgcagaat gctcaaaaac agttttcgtg
    28681 cttgcggaat attacacagc ggttgttgca gatggcgacg ggcatggcgt aactggcgca
    28741 gcgtggtgga gtaaatgccg gtgatcgcac tgttgcgcac catggtgtag aacatacgca
    28801 agatggcttc cggctggcgc ataaatagtg tttcatcacg caggtcgatt agcgtaccgc
    28861 gtagctgaaa ctcatcgtcg agtggacgtg gtttttcgtc ggcgggaagg gcgaggatgg
    28921 cttcatcgaa cagttgcagc agcatctggt tgagttcact gacacggcgt gtaacgcgga
    28981 agtaatcctt catcatccgc tcgaccggtt cgttgccttc accactgtaa ttcagacgct
    29041 gagcgacgct aagttggcga tcgaataaca gacgattatc gtaacggctg acgaccagat
    29101 gcagggcaaa gcgaatacgc cacagtatat gcagacattc gtttaattcc gcccgctccg
    29161 ctgaggttaa gaagccaaac ccgaccattt catccagcga tgttgcgcca aaatgacggc
    29221 gggccaccca ttgcagagtg tgaatatcgc gcaagccgcc agggctactt ttgatgtctg
    29281 gttcaaggtt gtagctggtg ccatggtaac gctgatggcg ctggttctgt tcttcaactt
    29341 tcgctgcgta gaacttgtcg gaaggccaga atccttcgct aaaaatatgt ttttgcagtt
    29401 cgaggaagag cgcaacatcg ccaattaata agcgggattc gattaaattg gtggcgacgg
    29461 ttaaatccga taacccttcc aacatgcact cttcaagcgt gcgcacacta tgaccgactt
    29521 ccagctttac atcccagagc agcgttaaca gctcgcccac tttttgcgcc tgatcgtccg
    29581 ggagcttttt acggcttaaa atcagtaaat cgatgtcaga aagtggatgc agctcgccac
    29641 gaccgtagcc accgacggca accaatgcca ggtcggcaat ctggctgaat cccgcttcaa
    29701 tccataatcg ttgcaggagc tggtctatga actcggtacg tgcctcaatc aactgttctg
    29761 cagagatccc attgtcaaag gcatcaccca gccaacgctg gaaagtatcg atatgggctt
    29821 ttatcccaca gacggttaat tcatcacggg gccaggcgca tggattttgc ggttgaccgg
    29881 gcagggtgga gagagcggtg tttgcgtact gttctggaag ggtattcatt gtgcgccacc
    29941 cataagatta aattatcgca ttaaaaaagc cggcattcgc cggcttcatc ttattcgtcg
    30001 tgcgagatta tcgccgggat ggtgtcatcc ttgcgtagcg tcaaaatttc gcagccgtta
    30061 tcagtcacca caatagtatg ctcatattgt gcagacaagc tgcgatcttt ggtttttacc
    30121 gtccagccat ctttcatggt gcggatctct tttttacccg cgttgaccat tggctcaatg
    30181 gtgaacgtca tccccggttt cagcacgacg ttggtttcac gggagtcata gtgcagcacc
    30241 tgcggttctt catggaagcc gcgaccaata ccgtgtccgc aatattcacg aacgacggag
    30301 aagccttctg cttcgacaaa tttctgaatc gccgcaccga tttcgcgcag attaatgcct
    30361 ggttttacca tgcgtagcgc caggtacagg ctttcttgcg tgatgcggca caggcgttcg
    30421 cccatgatgg tcggcttacc gacgataaac attttcgagg tatcgccgtg gaaaccatct
    30481 ttgattacgg tgacatcaat gttaacgata tcgccatctt tcagcagctt agcatcgtcc
    30541 gggataccgt ggcacaccac ttcattaata gagatgcaaa cggatttcgg atagccgtga
    30601 tagccgaggc aggcagaaac cgcgtgttgt tcattaacaa tgtaatcatt acagatgcga
    30661 tccagctcgc cggtgctgac gcccggttta acatacggtt cgatcatctc cagcacttcg
    30721 gcagccagtc ggccagcgac gcgcattttt tcgatatctt ctggggtctt gattgagata
    30781 gccattaatt ctgtccatca gcgtcggtga taccgacaat atatatgtaa gtgccgtcaa
    30841 tggtatcaca cccgggcaaa ttgagaatca ttctgaattt cgccaaacgt gccactgaag
    30901 gttttctata atagaaaatt cgacgtctga tgctgtacac agcgccaaca attattggtg
    30961 tccacgacgt atttgtggta taaagcgcgc cggacttccg atccatttcg tatacacaga
    31021 ctggacggaa gcgacaatct cactttgtgt aacaacacac acgtatcggc acatattccg
    31081 gggtgccctt tggggtcggt aatatgggat acgtggaggc ataaccccaa cttttatata
    31141 gaggttttaa tcatggcaac tgtttccatg cgcgacatgc tcaaggctgg tgttcacttc
    31201 ggtcaccaga cccgttactg gaacccgaaa atgaagccgt tcatcttcgg tgcgcgtaac
    31261 aaagttcaca tcatcaacct tgagaaaact gtaccgatgt tcaacgaagc tctggctgaa
    31321 ctgaacaaga ttgtttctcg caaaggtaaa atccttttcg ttggtactaa acgcgctgca
    31381 agcgaagcgg tgaaagacgc tgctctgagc tgcgaccagt tcttcgtgaa ccatcgctgg
    31441 ctgggcggta tgctgactaa ctggaaaacc gttcgtcagt ccatcaaacg tctgaaagac
    31501 ctggaaactc agtctcagga cggtactttc gacaagctga ccaagaaaga agcgctgatg
    31561 cgcactcgtg agctggagaa actggaaaac agcctgggcg gtatcaaaga catgggcggt
    31621 ctgccggacg ctctgtttgt aatcgatgct gaccacgaac acattgctat caaagaagca
    31681 aacaacttgg gtattccggt atttgctatc gttgatacca actctgatcc ggacggtgtt
    31741 gacttcgtta tcccgggtaa cgacgacgca atccgtgctg tgaccctgta cctgggcgct
    31801 gttgctgcaa ccgtacgtga aggccgttct caggatctgg cttcccaggc ggaagaaagc
    31861 ttcgtagaag ctgagtaata aggcttgata actcccccaa aatagttcga gttgcagaaa
    31921 ggcggcaagc tcgagaattc ccgggagctt acatcagtaa gtgaccggga tgagcgagcg
    31981 aagacaacgc atctgcggcg cgaaatatga agggggagag cccttataga ccaggtagta
    32041 cacgtttggt tagggggcct gcatatggcc ccctttttca cttttatatc tgtgcggttt
    32101 aatgccgggc agatcacatc tccgaggatt ttagaatggc tgaaattacc gcatccctgg
    32161 taaaagagct gcgtgagcgt actggcgcag gcatgatgga ttgcaaaaaa gcactgactg
    32221 aagctaacgg cgacatcgag ctggcaatcg aaaacatgcg taagtccggt gctattaaag
    32281 cagcgaaaaa agcaggcaac gttgctgctg acggcgtgat caaaaccaaa atcgacggca
    32341 actacggcat cattctggaa gttaactgcc agactgactt cgttgcaaaa gacgctggtt
    32401 tccaggcgtt cgcagacaaa gttctggacg cagctgttgc tggcaaaatc actgacgttg
    32461 aagttctgaa agcacagttc gaagaagaac gtgttgcgct ggtagcgaaa attggtgaaa
    32521 acatcaacat tcgccgcgtt gctgcgctgg aaggcgacgt tctgggttct tatcagcacg
    32581 gtgcgcgtat cggcgttctg gttgctgcta aaggcgctga cgaagagctg gttaaacaca
    32641 tcgctatgca cgttgctgca agcaagccag aattcatcaa accagaagac gtatccgctg
    32701 aagtggtaga aaaagaatac caggtacagc tggatatcgc gatgcagtct ggtaagccga
    32761 aagaaatcgc agagaaaatg gttgaaggcc gcatgaagaa attcaccggc gaagtttctc
    32821 tgaccggtca gccgttcgtt atggaaccaa gcaaaactgt tggtcagctg ctgaaagagc
    32881 ataacgctga agtgactggc ttcatccgtt tcgaagtggg tgaaggcatc gagaaagttg
    32941 agactgactt tgcagcagaa gttgctgcga tgtccaagca gtcttaatta tcaaaaagga
    33001 gccgcctgag ggcggcttct ttttgtgccc atcttgtaaa ttcagctaac ccttgtgggg
    33061 ctgcgctgaa aagcgacgta caatgtcgct agtattaatt catttcaatc gttgacagtc
    33121 tcaggaaaga aacatggcta ccaatgcaaa acccgtctat aaacgcattc tgcttaagtt
    33181 gagtggcgaa gctctgcagg gcactgaagg cttcggtatt gatgcaagca tactggatcg
    33241 tatggctcag gaaatcaaag aactggttga actgggtatt caggttggtg tggtgattgg
    33301 tgggggtaac ctgttccgtg gcgctggtct ggcgaaagcg ggtatgaacc gcgttgtggg
    33361 cgaccacatg gggatgctgg cgaccgtaat gaacggcttg gcaatgcgtg atgcactgca
    33421 ccgcgcctat gtgaacgctc gtctgatgtc cgctattcca ttgaatggcg tgtgcgatag
    33481 ctacagctgg gcagaagcta tcagcctgtt gcgcaacaac cgtgtggtga tcctctccgc
    33541 cggtacaggt aacccgttct ttaccaccga ctcagcagct tgcctgcgtg gtatcgaaat
    33601 tgaagccgat gtggtgctga aagcaaccaa agttgacggc gtgtttaccg ctgatccggc
    33661 gaaagatcca accgcaacca tgtacgagca actgacttac agcgaagtgc tggaaaaaga
    33721 gctgaaagtc atggacctgg cggccttcac gctggctcgt gaccataaat taccgattcg
    33781 tgttttcaat atgaacaaac cgggtgcgct gcgccgtgtg gtaatgggtg aaaaagaagg
    33841 gactttaatc acggaataat tcccgtgatg gataaataag ggtaagattc cgcgtaagta
    33901 tcgcgggggc gtaagtctgg ttataaggcg ttattgttgc aggcagtttg gtcacggcca
    33961 gcgcgcagca accggagcgt acaaaagtac gtgaggatgg cgagcactgc tcggggccaa
    34021 aatggcaaat aaaatagcct aataatccag acgattaccc gtaatatgtt taatcagggc
    34081 tatacttagc acacttccac tgtgtgtgac tgtctggtct gactgagaca agttttcaag
    34141 gattcgtaac gtgattagcg atatcagaaa agatgctgaa gtacgcatgg acaaatgcgt
    34201 agaagcgttc aaaacccaaa tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct
    34261 ggatggcatt gtcgtggaat attacggcac gccgacgccg ctgcgtcagc tggcaagcgt
    34321 aacggtagaa gattcccgta ctctgaaaat caacgtgttt gatcgttcaa tgtctccggc
    34381 cgttgaaaaa gcgattatgg cctccgatct cggcctgaac ccgaactctg cgggtagcga
    34441 catccgtgtt ccgctgccgc cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt
    34501 tcgtggtgaa gcagaacaag cgcgtgttgc agtacgtaat gtgcgtcgtg acgcgaacga
    34561 caaagtgaaa gcactgttga aagataaaga gatcagcgaa gacgacgatc gccgttctca
    34621 ggacgatgta cagaaactga ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga
    34681 caaagaagca gaactgatgc agttctgatt tcttgaacga cgaaaacgcc gctcagtaga
    34741 tccttgcgga tcggctggcg gcgttttgct ttttattctg tctcaactct ggatgtttca
    34801 tgaagcaact caccattctg ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg
    34861 tgcgccataa tcccgaacac ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc
    34921 gcatggtaga acagtgcctg gaattctctc cccgctatgc cgtaatggac gatgaagcga
    34981 gtgcgaaact tcttaaaacg atgctacagc aacaggggag ccgcaccgaa gtcttaagtg
    35041 ggcaacaagc cgcttgcgat atggcagcgc ttgaggaggt tgatcaggtg atggcagcca
    35101 ttgttggtgc tgctgggctg ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt
    35161 tgctggccaa taaagaatca ctggttacct gcggacgtct gtttatggac gccgtaaagc
    35221 agagcaaagc gcaattgtta ccggtcgata gcgaacataa cgctattttt cagagtttac
    35281 cgcaacctat ccagcataat ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca
    35341 ttttacttac cgggtctggt ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa
    35401 tgacgcccga tcaagcttgc cgtcatccga actggtcgat gggacgtaaa atttctgtcg
    35461 attcggctac catgatgaac aaaggtctgg aatacattga agcgcgttgg ctgtttaacg
    35521 ccagcgccag ccagatggaa gtgctgattc acccgcagtc agtgattcac tcaatggtgc
    35581 gctatcagga cggcagtgtt ctggcgcagc tgggggaacc ggatatgcgt acgccaattg
    35641 cccacaccat ggcatggccg aatcgcgtga actctggcgt gaagccgctc gatttttgca
    35701 aactaagtgc gttgacattt gccgcaccgg attatgagcg ttatccatgc ctgaaactgg
    35761 cgatggaggc gttcgaacaa ggccaggcag cgacgacagc attgaatgcc gcaaacgaaa
    35821 tcaccgttgc tgcttttctt gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt
    35881 tatccgtact ggaaaaaatg gatatgcgcg aaccacaatg tgtggacgat gtgttatctg
    35941 ttgatgcgaa cgcgagtgaa gtcgccagaa aagaggtgat gcgtctcgca agctgaggat
    36001 aatccggcta cagagagtcg cgctatttgt tagcgtaggg cttcagtgat atagtctgcg
    36061 ccatctgatc gtaagtagtt ggctttataa ggtcaggtat gccgtggttt tacacggctt
    36121 ttttttgtat aggcttcagt attcctgagt accgtaaacc ctgtcaggga ataaaaaacg
    36181 cgtgatgttg tctgctactc aaccacttag cgaaaaattg ccagcgcatg gctgccgtca
    36241 tgttgcgatc attatggacg gcaatggtcg ctgggcaaaa aagcaaggga agattcgtgc
    36301 ctttgggcat aaagccgggg caaaatccgt ccgccgggct gtctcttttg cggccaacaa
    36361 cggtattgag gcgttaacgc tgtatgcctt tagtagtgaa aactggaacc gaccagcgca
    36421 ggaagtcagt gcgttaatgg aactgtttgt gtgggcgctc gatagcgaag taaaaagtct
    36481 gcaccgacat aacgtgcgtc tgcgtattat tggcgatacc agtcgtttta actcgcgttt
    36541 gcaagaacgt attcgtaaat ctgaagcgct aacagccgga aataccggtc tgacgctgaa
    36601 tattgcggcg aactacggtg gacgttggga tatagtccag ggagtcaggc aactggctga
    36661 aaaggtgcag caaggaaacc tgcaaccaga tcagatagat gaagagatgt taaaccagca
    36721 tgtctgtatg catgaactgg cccctgtaga tttagtaatt aggactgggg gagagcatcg
    36781 cattagtaac tttttgcttt ggcaaattgc ctatgccgaa ctttacttta cagatgttct
    36841 ctggcccgat ttcgatgaac aagactttga aggggcgtta aatgcctttg ctaatcgaga
    36901 gcgtcgtttc ggcggcaccg agcccggtga tgaaacagcc tgatgggggt cgcttttgct
    36961 gaagtatcgc ctgatatctg cttttgtgtt aatacccgtc gtcatcgcgg cgttgtttct
    37021 gttgccgccg gtggggttcg ccattgtaac gctggtggtc tgcatgctgg cagcgtggga
    37081 atggggacag cttagcggtt ttaccactcg ttcgcagcga gtatggttgg cggtgttatg
    37141 cgggttattg ttggcgctga tgctttttct gttgccggaa tatcaccgaa atattcatca
    37201 accgctggtt gaaatctcac tttgggcttc gttgggttgg tggattgtcg cgctattgct
    37261 ggtgctgttt tacccaggtt ccgcagcaat ctggcgtaac tctaaaacat tgcgccttat
    37321 ttttggcgtg ctaaccattg ttcccttctt ctggggcatg ctggcgttac gggcctggca
    37381 ctatgacgag aatcattaca gtggcgcaat atggctgctc tatgtcatga tcctggtatg
    37441 gggcgctgac tccggcgcat atatgtttgg caaattgttt ggtaaacata agctggcacc
    37501 gaaggtttct ccgggtaaaa cctggcaagg ctttataggt ggactcgcta ctgcagcggt
    37561 aatctcatgg ggttatggca tgtgggcgaa tctcgacgtc gctcccgtca ccttactcat
    37621 ttgctctatt gtcgcagcgt tagcctcagt gctcggcgat ctgaccgaga gtatgtttaa
    37681 gcgtgaagca ggaattaagg acagcggtca tttaattcca ggacacggtg gtattttaga
    37741 tcgtattgat agcctgacgg ctgcggtacc ggtctttgct tgcttgttgt tactggtatt
    37801 caggacgctt taacggaagg taatatgctg agttttctct gggatttggc ttcgttcatc
    37861 gttgcactgg gtgtacttat caccgtgcat gaatttggtc atttctgggt tgcccggcgt
    37921 tgtggtgttc gcgttgagcg tttctcaata gggtttggta aggcgctctg gcggcgaact
    37981 gataagctcg gcaccgaata tgttatcgcc ctgatcccgt tgggcggtta tgtcaaaatg
    38041 ctggatgagc gcgcagaacc ggtcgttccg gaactccgcc accatgcctt caataataaa
    38101 tctgtcggcc aacgagcggc gattattgcc gcaggtccgg ttgcaaactt catttttgct
    38161 atctttgcct actggctggt ttttattatt ggtgtgcctg gcgtacgtcc ggtggttggc
    38221 gaaatagcag ccaattcgat agctgcggaa gcacaaattg caccaggtac ggaactaaaa
    38281 gccgtagatg gtatcgaaac gcctgattgg gatgccgtgc gtttgcagtt ggtcgataaa
    38341 attggcgatg aaagcaccac cattacagta gcgccatttg gcagcgacca acggcgggat
    38401 gtaaagctcg atttacgtca ctgggcgttt gagcctgata aagaagatcc ggtatcttcg
    38461 ctggggattc gtcctcgtgg gccgcaaatt gaacctgtac tggaaaatgt gcagccaaac
    38521 tcggcggcaa gcaaggcagg tttgcaagca ggcgacagga tcgttaaagt cgatggtcag
    38581 cccttaacgc agtgggtgac ctttgtgatg cttgtccggg ataacccggg taaatcctta
    38641 gcgttagaaa tcgaaaggca ggggagtccc ttgtctttga cattaatccc ggggagtaaa
    38701 ccgggtaatg gtaaagcgat tggttttgtc ggtattgagc cgaaagtcat tcctttgcca
    38761 gatgagtata aagttgtacg ccagtatggg ccgttcaacg ccatcgtcga agccacggac
    38821 aaaacgtggc agctgatgaa gctgacggtc agtatgctgg gaaaattgat caccggtgat
    38881 gtgaaactga acaacctcag tgggccgatc tctatcgcca agggggctgg gatgacagcg
    38941 gaactcgggg tagtttatta cctgccgttt cttgcgctta ttagcgtgaa cttagggata
    39001 attaacctgt ttccgttgcc cgtacttgac ggggggcatc tgctgttcct tgcgatcgaa
    39061 aagatcaagg gcggaccggt atccgagcgg gttcaagact tttgttatcg cattggctcg
    39121 attctgctgg tgctgttaat ggggcttgca cttttcaatg atttctctcg gttatgagag
    39181 ttagttagga agaacgcata ataacgatgg cgatgaaaaa gttgctcata gcgtcgctgc
    39241 tgtttagcag cgccaccgta tacggtgctg aagggttcgt agtgaaagat attcatttcg
    39301 aaggccttca gcgtgtcgcc gttggtgcgg ccctcctcag tatgccggtg cgtacaggcg
    39361 acacggttaa tgatgaagat atcagtaata ccattcgcgc tctgtttgct accggcaact
    39421 ttgaggatgt tcgcgtcctt cgtgatggtg atacccttct ggttcaggta aaagaacgtc
    39481 cgaccattgc cagcattact ttctccggta acaaatcggt gaaagatgac atgctgaagc
    39541 aaaacctcga ggcttctggt gtgcgtgtgg gcgaatccct cgatcgcacc accattgccg
    39601 atatcgagaa aggtctggaa gacttctact acagcgtcgg taaatatagc gccagcgtta
    39661 aagctgtcgt gaccccgctg ccgcgcaacc gtgttgacct aaaactggtg ttccaggaag
    39721 gtgtgtcagc tgaaatccag caaattaaca ttgttggtaa ccatgctttc accaccgacg
    39781 aactgatctc tcatttccaa ctgcgtgacg aagtgccgtg gtggaatgtg gtaggcgatc
    39841 gtaaatacca gaaacagaaa ctggcgggcg atcttgaaac cctgcgcagc tactatctgg
    39901 accgcggtta tgcccgtttc aacatcgact ctacccaggt cagtctgacg ccagataaaa
    39961 aaggtattta cgtcacggtg aacatcaccg aaggcgatca gtacaagctt tctggcgttg
    40021 aagtgagcgg caaccttgcc gggcactccg ctgaaattga gcagctgact aagatcgagc
    40081 cgggcgagct gtataacggc actaaagtga ccaaaatgga agatgacatt aaaaagcttc
    40141 tcggtcgcta tggttatgcc tatccgcgcg tacagtcgat gcctgaaatt aacgatgccg
    40201 acaaaaccgt taaattacgc gtgaacgttg atgtgggtaa ccgtttctac gtgcgtaaga
    40261 tccgttttga aggtaacgat acctcgaaag atgccgtcct gcgtcgcgaa atgcgtcaga
    40321 tggaaggtgc atggttgggg agcgatctgg tcgatcaggg taaggagcgt ctgaatcgtc
    40381 tgggcttctt tgaaactgtc gataccgata cccaacgtgt tccgggtagc ccggaccagg
    40441 ttgatgtagt ctacaaggtg aaagaacgta acactggtag cttcaacttc gggattggtt
    40501 acggtactga aagtggcgtg agcttccagg ctggcgtgca acaggataac tggttaggta
    40561 caggttatgc tgttggtatc aacgggacca aaaacgatta ccagacctat gctgaactgt
    40621 cggtaaccaa cccgtacttc accgtagatg gcgtaagcct cggtggtcgt ctcttctata
    40681 atgacttcca ggcagatgac gccgacctgt ccgactatac caacaagagt tatggtacag
    40741 acgtgacgtt gggcttcccg attaacgaat ataactcgct gcgtgcaggt ctgggttatg
    40801 tacataactc cctgtccaac atgcagcctc aggttgcgat gtggcgttat ctgtactcta
    40861 tgggtgaaca tccgagcacc tctgatcagg ataacagctt caaaacggac gacttcacgt
    40921 tcaactatgg ttggacctat aacaagcttg accgtggtta cttcccgaca gatggttcac
    40981 gtgtcaacct gaccggtaaa gtgaccattc ctggatcgga taacgaatac tacaaagtga
    41041 cgttagacac ggcgacttat gtgccgatcg atgacgatca caaatgggtt gttctgggtc
    41101 gtacccgctg gggttatggt gatggtttag gcggcaaaga gatgccgttc tacgagaact
    41161 tctatgccgg tggttccagc accgtgcgtg gcttccagtc caataccatt ggtccgaaag
    41221 cagtttactt cccgcatcag gccagtaatt atgatccgga ctatgattac gaatgtgcga
    41281 ctcaggacgg cgcgaaagac ctgtgtaaat cggatgatgc tgtaggcggt aacgccatgg
    41341 cggttgccag cctcgagttc atcaccccga cgccgtttat tagcgataag tatgctaact
    41401 cggttcgtac ttcattcttc tgggatatgg gtaccgtttg ggatacaaac tgggattcca
    41461 gccaatattc tggatatccg gactatagtg atccaagcaa tatccgtatg tctgcgggta
    41521 tcgcattaca atggatgtcc ccattggggc cgttggtgtt ctcctacgcc cagccgttca
    41581 aaaagtacga tggagacaag gcagaacagt tccagtttaa catcggtaaa acctggtaag
    41641 tgttctccac aaaggaatgt agtggtagtg tagcgatgac tttaggcgat caatataaga
    41701 tcgccgggcc acgcaaagaa ctgcaccctc cggtgcaaat gggatggtaa ggagtttatt
    41761 gtgaaaaagt ggttattagc tgcaggtctt ggtttagcac tggcaacttc tgctcaggcg
    41821 gctgacaaaa ttgcaatcgt caatatgggc agcctgttcc agcaggtagc gcagaaaacc
    41881 ggtgtttcta acacgctgga aaatgagttc aaaggccgtg ccagcgaact gcagcgtatg
    41941 gaaaccgatc tgcaggctaa aatgaaaaaa ctgcagtcca tgaaagcggg cagcgatcgc
    42001 accaaactgg aaaaagacgt gatggctcag cgccagactt ttgctcagaa agcgcaggct
    42061 tttgagcagg atcgcgcacg tcgttccaac gaagaacgcg gcaaactggt tactcgtatc
    42121 cagactgctg tgaaatccgt tgccaacagc caggatatcg atctggttgt tgatgcaaac
    42181 gccgttgctt acaacagcag cgatgtaaaa gacatcactg ccgacgtact gaaacaggtt
    42241 aaataagtaa tgccttcaat tcgactggct gatttagcgc agcagttgga tgcagaacta
    42301 cacggtgatg gcgatatcgt catcaccggc gttgcgtcca tgcaatctgc acaaacaggt
    42361 cacattacgt tcatggttaa cccaaaatac cgtgagcatt taggcttgtg ccaggcgtcc
    42421 gcggttgtca tgacccagga cgatcttcct ttcgcgaaaa gtgccgcgct ggtagtgaag
    42481 aatccctacc tgacttacgc gcgcatggcg caaattttag ataccacgcc gcagcccgcg
    42541 cagaacattg cacccagtgc ggtgatcgac gcgacggcga agctgggtaa caacgtatcg
    42601 attggcgcta acgcggtgat tgagtccggc gttgaactgg gcgataacgt gattatcggt
    42661 gccggttgct tcgtaggtaa aaacagcaaa atcggtgcag gttcgcgtct ctgggcgaac
    42721 gtaaccattt accatgagat ccagatcggt cagaattgcc tgatccagtc cggaacagtg
    42781 gtaggcgcag acggctttgg ttatgccaac gatcgtggta actgggtgaa gatcccacag
    42841 attggtcgcg taattattgg cgatcgcgtg gagatcggtg cctgcacaac catcgatcgc
    42901 ggcgcgctgg atgacactgt tattggcaat ggcgtgatca ttgataacca gtgccagatt
    42961 gcacataacg tcgtgattgg cgacaatacg gcggttgccg gtggcgtcat tatggcgggc
    43021 agcctgaaaa ttggtcgtta ctgcatgatc ggcggagcca gcgtaatcaa cgggcatatg
    43081 gaaatatgcg acaaagtgac ggttacgggc atgggtatgg tgatgcgtcc catcactgaa
    43141 ccaggcgtct attcctcagg cattccgctg caatccaaca aagtctggcg caaaaccgct
    43201 gcactggtga tgaacattga tgacatgagc aagcgtctga aatcgcttga gcgcaaggtt
    43261 aatcaacaag actaacgttc catcttttgt tcgccaaact ttacggcctg tctcattctt
    43321 acgattgcgg caggccgtgt tattattgtc gtttcttata ttttgacagg aagagtatct
    43381 tgactactaa cactcatact ctgcagattg aagagatttt agaacttctg ccgcaccgtt
    43441 tcccgttctt actggtggat cgcgtgctgg attttgaaga aggtcgtttt ctgcgcgcag
    43501 taaaaaatgt ctctgtcaat gagccattct tccagggcca tttccctgga aaaccgattt
    43561 tcccgggtgt gctgattctg gaagcaatgg cacaggcaac aggtattctg gcgtttaaaa
    43621 gcgtaggaaa actggaaccg ggtgagctgt actacttcgc tggtattgac gaagcgcgct
    43681 tcaagcgccc ggtcgtgcct ggcgatcaaa tgatcatgga agtcactttc gaaaaaacgc
    43741 gccgcggcct gacccgtttt aaaggggttg ctctggtcga tggtaaagta gtttgcgaag
    43801 caacgatgat gtgtgctcgt agccgggagg cctgatacgt gattgataaa tccgcctttg
    43861 tgcatccaac cgccattgtg gaagagggcg cgtcaattgg cgcgaacgca cacattggtc
    43921 ctttttgtat cgttggaccc catgtcgaaa ttggtgaggg taccgtactg aaatctcacg
    43981 ttgtcgtgaa tggtcatact aaaattggcc gcgataatga gatttatcag tttgcctcca
    44041 tcggcgaagt taaccaggat ttgaaatatg ctggcgaacc gacccgtgtg gaaatcggcg
    44101 atcgtaaccg cattcgcgaa agcgtcacca ttcatcgtgg cacagtccag ggcggtggat
    44161 tgacgaaggt gggcagcgac aacttactga tgatcaacgc gcacattgcg cacgattgta
    44221 cggtaggtaa ccgctgtatt ctcgccaaca acgcaacgct ggcgggtcac gtatcggttg
    44281 acgacttcgc gatcatcggc ggcatgaccg cagtccatca gttctgcatc attggtgcgc
    44341 atgtgatggt tggcggctgc tccggtgtgg cgcaggacgt ccctccttat gtcattgcgc
    44401 agggtaacca cgcaacgccg ttcggtgtca atatcgaagg gctgaagcgc cgcggattca
    44461 gccgtgaggc gattaccgct atccgcaatg cgtataagct aatttatcgt agcggtaaaa
    44521 cgctcgatga agtgaaaccg gaaattgctg aactggcgga aacatatccg gaagtgaaag
    44581 cctttaccga tttctttgca cgctcaacgc gcggtctgat tcgttaatga ctgaccagcg
    44641 tccattaacg attgccctgg tcgccggaga aacctccggc gatatcctgg gggccggttt
    44701 aatccgcgct ctgaaagaac gtgtgcccaa cgcccgcttt gttggtgttg ccgggccacg
    44761 aatgcaggct gaaggctgcg aagcctggta cgaaatggaa gaactggcgg tgatgggcat
    44821 tgttgaagtg ctcggtcgtc tgcgtcgctt actgcatatt cgtgccgatc tgacaaagcg
    44881 ttttggcgaa ctgaagccag atgtttttgt tggtattgat gcgcccgact tcaatattac
    44941 tcttgaaggt aacctcaaaa agcagggtat caaaaccatt cattacgtca gtccatccgt
    45001 ctgggcgtgg cgacagaaac gcgttttcaa aataggcaga gccaccgatc tggtgctcgc
    45061 atttctgcct ttcgaaaaag cgttttatga caaatacaat gtaccgtgcc gctttatcgg
    45121 tcataccatg gctgatgcca tgccgttaga tccagataaa aatggtgccc gtgatgtgct
    45181 ggggatccct tacgatgccc actgtctggc attgttgccg ggcagccgtg gtgcagaagt
    45241 cgaaatgctt agtgccgatt tcttgaaaac tgcccagctt ttgcgccaga catatccgga
    45301 tctcgaaatc gtggtgccgc tggtgaatgc caaacgccgc gagcagtttg aacgcatcaa
    45361 agctgaagtc gcgccagacc tgtcggttca tttgctggat ggaatgggcc gtgaggcgat
    45421 ggtcgccagc gatgcggcac tactggcgtc ggggacggca gccctggagt gtatgctggc
    45481 gaaatgcccg atggtggtgg gatatcgcat gaagcctttt accttctggt tggcgaagcg
    45541 gctggtgaaa actgattatg tctcgctgcc aaatctgctg gcgggcagag agttagtcaa
    45601 agagttattg caggaagagt gtgagccgca aaaactggct gcggcgctgt taccgctgtt
    45661 ggcgaacggg aaaaccagcc acgcgatgca cgataccttc cgtgaactgc atcagcagat
    45721 ccgctgcaat gccgatgagc aggcggcaca agccgttctg gagttagcac aatgatcgaa
    45781 tttgtttatc cgcacacgca gctggttgcg ggtgtggatg aagtcggacg cgggccgtta
    45841 gttggcgcgg tcgtcaccgc tgcggtgatc cttgacccgg cgcgcccgat tgccgggctg
    45901 aatgattcca aaaagctgag cgaaaaacgc cgtctggcgc tctatgaaga gatcaaagag
    45961 aaagcgttga gctggagtct gggccgcgcg gaaccccacg aaatcgacga gctgaacatt
    46021 cttcatgcga ccatgctggc gatgcagcgt gccgtcgctg ggctgcatat tgcgccggaa
    46081 tatgtgttga ttgatggtaa ccgctgcccg aaattaccga tgcctgcgat ggctgtggtg
    46141 aaaggggata gccgcgtacc agaaatcagt gccgcgtcta tcctggcgaa agtgacgcgt
    46201 gacgccgaaa tggcggcgct ggatattgtt ttcccgcaat atggttttgc ccaacataaa
    46261 gggtatccaa ccgcttttca tctggaaaaa ctggctgaat atggcgcgac tgaacatcat
    46321 cgtcgcagct ttgggcctgt caaacgcgca ctgggacttg cgtcctgatt cttgtgtcga
    46381 gattaagtaa accggaatct gaagatgtct gaaccacgtt tcgtacacct gcgggtgcac
    46441 agcgactact cgatgatcga tggcttggcc aaaaccgcac cgctggtaaa aaaggcggcg
    46501 gcgttgggta tgccagcact ggcgatcacc gatttcacca acctttgcgg tctggtgaag
    46561 ttctacggag cgggacatgg cgcagggatt aagcctatcg tcggggcaga ttttaatgtc
    46621 cagtgcgacc tgctgggtga tgagttaacc cacctgacgg tactggcggc gaacaatacc
    46681 ggctatcaga atctggcgtt gctgatctca aaagcgtatc agcgcgggta cggtgccgcc
    46741 gggccgatca tcgatcgcga ctggcttatc gaattaaacg aagggttgat ccttctttcc
    46801 ggcgggcgca tgggcgacgt cggacgcagt cttttgcgtg gtaacagcgc gctggtagat
    46861 gagtgtgtcg cgttttatga agaacacttc ccggatcgct attttctcga gctgatccgc
    46921 accggcaggc cggatgaaga aagctatctg cacgcggcgg tggaactggc ggaagcgcgc
    46981 ggtttgcccg tcgtggcgac taacgatgtg cgctttatcg acagcagcga ctttgacgca
    47041 cacgaaatcc gcgtcgcgat ccacgacggc tttaccctcg acgatcctaa acgcccgcgt
    47101 aactattcgc cgcagcaata tatgcgtagc gaagaggaga tgtgtgagct gtttgccgac
    47161 atcccagaag cccttgccaa caccgttgag atcgccaaac gctgtaacgt aactatgcgt
    47221 cttggtgaat acttcctgcc gcagttcccg accggggaca tgagcaccga agattatctg
    47281 gtcaagcgtg caaaagaagg cctggaagag cgtctggcat ttttattccc tgacgaggaa
    47341 gaacgtctta aacgccgccc ggaatatgac gaacgtctgg agactgaact tcaggttatc
    47401 aaccagatgg gcttcccggg ctacttcctc atcgttatgg aatttatcca gtggtcgaaa
    47461 gataacggcg taccggtagg gccaggccgt ggctccggtg cgggttcact ggtggcctac
    47521 gcgctgaaaa tcaccgacct cgatccgctg gaatttgacc tgctgttcga acgtttcctt
    47581 aacccggaac gtgtctccat gcctgacttc gacgttgact tctgtatgga gaaacgcgat
    47641 caggttatcg aacacgtggc ggacatgtac ggtcgtgatg cggtatcgca gattatcacc
    47701 ttcggtacga tggcggcgaa agcggtgatc cgcgacgtag gccgcgtgct ggggcatccg
    47761 tacggctttg tcgaccgtat ctcgaaactg atcccgcccg atccggggat gacgctggcg
    47821 aaagcgtttg aagccgagcc gcagctgccg gaaatctacg aagcggatga agaagttaag
    47881 gcgctgatcg acatggcgcg caaactggaa ggggtcaccc gtaacgccgg taagcacgcc
    47941 ggtggggtgg ttatcgcgcc gaccaaaatt accgattttg cgccgcttta ttgcgatgaa
    48001 gagggcaaac atccggtcac ccagtttgat aaaagcgacg ttgaatacgc cggactggtg
    48061 aagttcgact tccttggttt gcgtacgctc accatcatca actgggcgct ggagatgatc
    48121 aacaagcggc gggcgaagaa tggcgagcca ccgctggata tcgccgcgat cccgctggat
    48181 gacaagaaaa gcttcgacat gctgcaacgc tcggaaacca cggcggtatt ccagcttgaa
    48241 tcgcgcggca tgaaggacct gatcaagcgt ctgcaacctg actgcttcga agatatgatc
    48301 gcactggtgg cactgttccg ccctggtccg ttgcagtcag ggatggtgga taactttatc
    48361 gaccgtaaac atggtcgtga agagatctcc tatccggacg tgcagtggca gcatgaaagc
    48421 ctgaaatcgg tactggagcc aacctacggc attatcttgt atcaggaaca ggtcatgcag
    48481 attgcccagg tgctttctgg ttataccctc ggtggcgcgg atatgctgcg ccgtgcgatg
    48541 ggtaagaaaa agccggaaga gatggctaag cagcgttctg tatttgctga aggtgcagaa
    48601 aagaacggaa tcaacgccga actggcgatg aaaatcttcg acctggtgga gaaattcgcg
    48661 ggttacggat ttaacaaatc gcactctgcg gcctatgctt tggtgtcata tcaaacgtta
    48721 tggctgaaag cgcactatcc ggcggagttt atggcggcgg taatgaccgc cgatatggac
    48781 aacaccgaga aggtggtggg cctggtggat gagtgctggc ggatggggct gaaaatcctg
    48841 ccaccagata taaactccgg tctttaccat ttccacgtca acgacgacgg cgaaatcgtg
    48901 tatggtattg gcgccatcaa aggggtaggt gaaggtccga ttgaggccat catcgaagcc
    48961 cgtaataaag gcggctactt ccgcgaactg tttgatctct gcgcccgtac cgacaccaaa
    49021 aagttaaacc ggcgggtgct ggaaaaactg atcatgtccg gggcgtttga ccgtcttggg
    49081 ccacaccgcg cggcgctgat gaactcgctg ggtgatgcgt taaaagcggc agatcaacac
    49141 gcgaaagcgg aagctatcgg tcaggccgat atgttcggcg tgctggccga agagccggaa
    49201 caaattgaac aatcctacgc cagctgccaa ccgtggccgg agcaggtggt attagatggg
    49261 gaacgtgaaa cgttaggcct gtacctgacc ggacacccta tcaaccagta tttaaaagag
    49321 attgagcgtt atgtcggagg cgtaaggctg aaagacatgc acccgacaga acgtggtaaa
    49381 gtcatcacgg ctgcggggct cgttgttgcc gcgcgggtta tggtcaccaa gcgcggcaat
    49441 cgtatcggta tctgcacgct ggatgaccgt tccgggcggc tggaagtgat gttgtttact
    49501 gacgccctgg ataaatacca gcaattgctg gaaaaagacc gcatacttat cgtcagcgga
    49561 caggtcagct ttgatgactt cagcggtggg cttaaaatga ccgctcgcga agtgatggat
    49621 attgacgaag cccgggaaaa atatgctcgc gggcttgcta tctcgctgac ggacaggcaa
    49681 attgatgacc agcttttaaa ccgactccgt cagtctctgg aaccccaccg ctctgggaca
    49741 attccagtac atctctacta tcagagggcg gatgcacgcg cgcggttgcg ttttggcgcg
    49801 acgtggcgtg tctctccgag cgatcgttta ttaaacgatc tccgtggcct cattggttcg
    49861 gagcaggtgg aactggagtt tgactaatac aggaatacta tgagtctgaa tttccttgat
    49921 tttgaacagc cgattgcaga gctggaagcg aaaatcgatt ctctgactgc ggttagccgt
    49981 caggatgaga aactggatat taacatcgat gaagaagtgc atcgtctgcg tgaaaaaagc
    50041 gtagaactga cgcgtaaaat cttcgccgat ctcggtgcat ggcagattgc gcaactggca
    50101 cgccatccgc agcgtcctta taccctggat tacgttcgcc tggcatttga tgaatttgac
    50161 gaactggctg gcgaccgtgc gtatgcagac gataaagcta tcgtcggtgg tatcgcccgt
    50221 ctcgatggtc gtccggtgat gatcattggt catcaaaaag gtcgtgaaac caaagaaaaa
    50281 attcgccgta actttggtat gccagcgcca gaaggttacc gcaaagcact gcgtctgatg
    50341 caaatggctg aacgctttaa gatgcctatc atcaccttta tcgacacccc gggggcttat
    50401 cctggcgtgg gcgcagaaga gcgtggtcag tctgaagcca ttgcacgcaa cctgcgtgaa
    50461 atgtctcgcc ttggcgtacc ggtagtttgt acggttatcg gtgaaggtgg ttctggcggt
    50521 gcgctggcga ttggcgtggg cgataaagtg aatatgctgc aatacagcac ctattccgtt
    50581 atctcgccgg aaggttgtgc gtccattctg tggaagagcg ccgacaaagc gccgctggcg
    50641 gctgaagcga tgggtatcat cgctccgcgt ctgaaagagc tgaaactgat cgactccatc
    50701 atcccggaac cgctgggcgg cgctcaccgt aacccggaag cgatggcggc atcgttgaaa
    50761 gcgcaactgc tggcggatct ggccgatctc gatgtgttaa gcactgaaga tttaaaaaat
    50821 cgtcgttatc agcgcctgat gagctacggt tacgcgtaat tcgcaaaagt tctgaaaaag
    50881 ggtcacttcg gtggcccttt tttatcgcca cggtttgagc aggctatgat taaggaagga
    50941 ttttccagga ggaacacatg aacatcattg ccattatggg accgcatggc gtcttttata
    51001 aagatgagcc catcaaagaa ctggagtcgg cgctggtggc gcaaggcttt cagattatct
    51061 ggccacaaaa cagcgttgat ttgctgaaat ttatcgaaca taaccctcga atttgcggcg
    51121 tgatttttga ctgggatgag tacagtctcg atttatgcag cgatatcaat caacttaatg
    51181 aatatctccc gctttatgcc ttcatcaaca cccactcgac gatggatgtc agcgtgcagg
    51241 atatgcggat ggcgctctgg ttttttgaat atgcgctggg gcaggcggaa gatatcgcca
    51301 ttcgtatgcg tcagtacacc aacgaatatc ttgataacat tacgccgccg ttcacgaaag
    51361 ccttgtttac ctacgtcaaa gagcggaagt acaccttttg tacgccgggg catatgggcg
    51421 gcaccgcata tcaaaaaagc ccggttggct gtctgtttta tgattttttc ggcgggaata
    51481 cccttaaggc tgatgtctct atttcggtca ccgagcttgg ttcgttgctt gaccacaccg
    51541 ggccacacct ggaagcggaa gagtacatcg cgcggacttt tggcgcggaa cagagttata
    51601 tcgttaccaa cggaacatcg acgtcgaaca aaattgtggg tatgtacgcc gcgccatccg
    51661 gcagtacgct gttgatcgac cgcaactgcc ataaatcact ggcgcatctg ttgatgatga
    51721 acgatgtagt gccagtctgg ctgaaaccga cgcgtaatgc gttggggatt ctggggggga
    51781 tcccgcgccg tgaatttact cgcgacagca tcgaagagaa agtcgctgcc actacgcaag
    51841 cacaatggcc ggttcatgcg gtgatcacca actccaccta tgatggtttg ctctacaaca
    51901 ccgactggat caaacagacg ctggatgtcc cgtcgatcca cttcgattcc gcctgggtgc
    51961 cgtatactca ttttcatcca atctaccagg gtaaaagtgg tatgagcggc gagcgtgttg
    52021 cgggaaaagt tttcttcgaa acgcaatcga cccacaaaat gctggcggcg tcatcgcagg
    52081 cttcactgat ccacattaaa ggcgagtatg acgaagaggc gtttaacgaa gcctttatga
    52141 tgcataccac cacctcgccc agttatccca ttgttgcttc ggttgagacg gcggcggcga
    52201 tgctgcgtgg taatccgggc aaacggctga ttaaccgttc ggtggagcgg gcgctgcatt
    52261 ttcgcaaaga ggtccagcgg ctgcgggaag agtctgacgg ttggtttttc gatatctggc
    52321 aaccgccgca ggtggatgaa gccgaatgca ggcccgttgc gcctggcgaa caatggcacg
    52381 gctttaacga tgcggatgcc gatcatatgt ttctcgatcc ggttaaagtc actattttga
    52441 caccggggat ggacgaacag ggcaatatga gtgaggaggg gatcccggcg gcgctggtag
    52501 caaaattcct cgacgaacgt gggatcgtag tagagaaaac cggcccttat aacctgctgt
    52561 ttctctttag tattggcatc gataaaacca aagcaatggg attattgcgt gggttgacgg
    52621 aatttaagcg ctcttacgat ctcaacctgc ggatcaaaaa tatgctgccc gatctctatg
    52681 ctgaagatcc cgatttctac cgcaatatgc gtattcagga tctggcgcaa gggatccata
    52741 agctgattcg taaacacgat ctttccggtt tgatgttgcg ggcattcgat actttgccgg
    52801 aaatgatcat gacgccacat caggcatggc agagacagat taaaggtgaa gtagaaacca
    52861 ttgcgctgga acaactggtc ggtagagtat cggcaaatat gatcctgcct tatccaccgg
    52921 gcgtaccgct gttgatgcca ggagaaatgc tgaccaaaga gagccgcacg gtactcgatt
    52981 ttctactgat gctttgttcc gtagggcaac attaccccgg ttttgaaacg gatattcacg
    53041 gcgcgaaaca ggacgaagac ggcgtttacc acgtacgagt cctaaaaatg gcgggataac
    53101 ttgccagagc ggcttccggg cgagtaacgt gctgttaaca aataaaggag acgttatgct
    53161 gggtttaaaa caggttcacc atattgcgat tattgcgacg gattatgcgg tgagcaaagc
    53221 tttctactgc gatattcttg gtttcacgct gcaaagcgaa gtctatcgcg aagcgcgcga
    53281 ctcgtggaaa ggggatttgg cgcttaatgg gcaatatgtg attgagcttt tctcttttcc
    53341 gttcccgccg gagcgcccca gccgaccgga agcttgcggc ctgcgtcatc tggcttttag
    53401 cgttgatgac atcgatgcgg cagtggcgca ccttgaaagc cataacgtga agtgtgaagc
    53461 catccgtgtc gatccataca cgcaaaaacg cttcaccttc tttaacgatc cggacgggct
    53521 gccgttggaa ctgtatgagc agtaaggctt gtcatcgccg catttgcccg gtaacgtgcc
    53581 gggcattgct actgtaaaat cgcaccatca tgacactcac gctcaataga caacttctca
    53641 cctcacgcca gattctggtg gcctttagcg gcgggcttga ctccaccgtt ctgctgcatc
    53701 agttggtgca gtggcggacg gaaaatccgg gcgtcactct gcgcgctatt catgtgcatc
    53761 acggtttaag tgccaatgcc gatgcctggg ttacgcattg cgaaaacgtc tgccaacagt
    53821 ggcaggtgcc gctggtggtc gaacgcgtac aacttgcgca agaggggctg gggattgagg
    53881 cccaggcgcg gcaggcacgt tatcaggcat ttgcccgtac cctattgccc ggcgaagtgc
    53941 tggtcaccgc gcaacatctc gacgatcaat gtgaaacctt tctgctggcg ctaaaacgcg
    54001 gtagcggccc tgccgggctt tcggctatgg cggaagtttc ggagtttgct ggaacacggc
    54061 ttatccgccc gttgcttgcc cgcacgcggg gggaactgga gcagtgggca ttggcgcatg
    54121 gtttacgctg gattgaagac gaaagtaatc aggacgactc atacgatcgt aactttctgc
    54181 gcctgcgcgt agtgccgtta ttgcagcagc gttggccgca ttttgcggaa gcaacggccc
    54241 gcagcgccac actttgtgct gaacaagaga gtctgttgga tgaactgctg gcggatgatt
    54301 tagcacactg ccaaacgtcg caggggacgc tgcagattgc gccaatgctg gcgatgagtg
    54361 atgcccgtcg cgcggcgatt attcgccgct ggctggcagg gcagaatgca ccgatgcctt
    54421 cccgcgacgc gctggtgcgc atctggcagg aagtggcgct ggcgcgggaa gatgcttccc
    54481 cctgtttacg tttgggcgcc tttgaaatcc gccgctatca gtcgcaactg tggtggatta
    54541 aatccgtcac cgggcaaagc gaaaccattg tgctgtggca gacgtggcta caaccgctgg
    54601 aattaccggc ggggctggga accgtacagc ttactgcggg aggcgatatt cgccctccgc
    54661 gtgcagacga agcggtcagc gtgcgtttca aagcgccagg attgctgcat attgtcgggc
    54721 gcaacggcgg acgtaagctg aagaaaatct ggcaagagtt aggcgtgccg ccgtggctac
    54781 gtgacaccac gccactgctg ttttatggag aaacgctgat tgcggcggca ggggtatttg
    54841 tgacgcaaga aggtgtggct gaaggtgaga atggcgtaag ttttgtctgg cagaaaacgc
    54901 ttagttaagt gaaagccgga taagacgcat caaacgtcgc atccggcgaa agtcaatcag
    54961 gattcgctta ccaccaccgt accgatttca gggtggctaa agctggtaat tttatccaga
    55021 cgcagctcgc gggtttcgcc agcggcctcg acgaccagat actccacatt tttgcgggag
    55081 actaaatcac tggcttttgc ctgcaatttt tcgccatctt tcagctcaag tgtcagcatt
    55141 aaatgatgct ggcaggcgag ctcaagatta tcgtaatcat cacaattaat tggttgatac
    55201 gtatcattca ttgacataat cgctcaccag taagtttgcc gcagcgtatg ctgctttttc
    55261 cctgacagcc tcagaaaggg cgtcgtcggc agccatttca ttcagcactt tcaaaacgca
    55321 gcccagcgcg tccggaacgt atcctaagtc tccgctggcg atttccgcgt accgcttgcg
    55381 tattaactca caatattttt ccacatgccc tcctgtcagc actctgactt aaccgtggat
    55441 gcaagtctaa gcctacgaag ataaactctg tttcgcaagg tgactatacc acactcattt
    55501 ctgcaatatc agcgccgcaa ctgcacgtat tccgttacaa tggcctcctg attcgaaagg
    55561 agttttctta tggcgcttaa agcgacaatt tataaagcga cggttaatgt ggccgatctc
    55621 gaccgcaacc agtttctcga tgcctctctg acgctggcgc gccacccttc agaaacccag
    55681 gagcgtatga tgctgcgctt gctggcgtgg ctgaaatatg ctgatgaacg tctgcaattt
    55741 acccgtggtt tgtgtgccga tgatgagccg gaagcgtggc tgcgtaacga tcacctgggc
    55801 attgatttgt ggattgagct ggggctgccg gatgagcgac gaattaagaa agcctgcacc
    55861 caggccgcag aagtggcgct gtttacctat aatagtcggg cggcgcaaat ctggtggcag
    55921 caaaatcaga gcaaatgtgt gcagtttgcc aatctttccg tctggtatct ggacgatgaa
    55981 caactggcga aagtaagcgc ctttgccgat cgtaccatga cgctgcaggc aacgattcag
    56041 gatggtgtta tctggttatc ggatgataag aataatctgg aagttaactt aaccgcctgg
    56101 caacaacctt catgattgtg atttcccgac atgttgctat tcccgatggt gagcttgaga
    56161 tcaccgccat tcgtgcgcag ggcgctggcg ggcagcatgt taataagacc tcaacggcta
    56221 ttcatctgcg ttttgacatt cgggcgtcca gcctgccaga gtattacaaa gagcgtctgc
    56281 tcgccgccag ccatcatttg atcagcagtg atggcgtgat tgtcattaag gcacaggaat
    56341 accgcagtca ggaactgaac cgcgaagccg ctctggcccg gctggtggca gtgattaaag
    56401 atttaacaaa agaacaaaaa gcccgacgac ccacgcggcc cacccgtgca tcgaaagagc
    56461 gcaggctggc atcgaaagca caaaaatcaa gcgtgaaggc gatgcgcggc aaagtacgca
    56521 gcggtcggga ataaaaagaa ggaatggatg gtgaaaaaag cgatagtgac agcgatggct
    56581 gtaatcagcc tctttactct gatgggatgt aataatcggg ccgaagtcga tacgctttct
    56641 ccggcgcagg ctgccgaact gaaaccgatg ccgcaaagtt ggcgcggcgt gctaccgtgt
    56701 gccgattgcg aaggaatcga aatctctctg ttcctcgaaa aagacggaac atgggtgatg
    56761 aatgagcgtt atctcggtgc tcgtgaagaa ccttcctcct tcgcttccta cggtacatgg
    56821 gcgcgaaccg ctgacaagct ggtattaacc gacagcaaag gtgaaaagtc atattatcgg
    56881 gcgaaaggcg atgcgctgga gatgctcgat cgtgaaggca atccaattga atcgcagttc
    56941 aactatacgc tggaaccggc acaatccagt ttacctatga cgccgatgac cctgcgaggc
    57001 atgtattttt atatggctga tgcggcgacc ttcactgatt gcgcgaccgg aaaacgtttc
    57061 atggtagcga ataacgcaga gctggagcgt agctacctgg ctgcgcgcgg tcacagtgaa
    57121 aaaccggtgt tactgtcagt agaaggtcac tttacgcttg aggctaatcc ggatactggt
    57181 gcgccaacca aagtattagc gcccgatacg gcgggtaaat tttacccgaa taaggattgc
    57241 agtagtttgg ggcagtaaac ctgcttaagg cagaaacaaa cgcaaaattg cctgatgcgc
    57301 tgcgcttatc aggcctacag catataatgc aacattttga attagcacga ttttgtaggc
    57361 cggataaggc gtttacgccg catccggcat tttgtgcacg aggctggctt tatcactgag
    57421 tcagcccaac aaagcgaccc gccttgatat aaatcccagg cttcaggtgc ccaacatact
    57481 gtaactgcgt ttctggctta aacgcagaga catcaccaat acgcatatgc atcagatcgg
    57541 cagggctaat ccagcccgat tgcgccagtg taagtggatg cccggatttg gcaaatgcat
    57601 cagtgacaaa ttccgaacaa aaccacgact ttttgtctcc ttcgcacaca ctgcttaact
    57661 gcgctttcgc caggtcgctg acgcactgtt ggcgaaaatc ctcggagaac ggattcagtg
    57721 agcacatctg gcgagtcacc ataaagggaa taaattcgac aatgccgcga tagttataac
    57781 cgctatcttt gattttattg gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat
    57841 ccgggactcg taagacgaaa agcttatcac tatgcttcat cgctttttta agggaaacaa
    57901 tctggacgcc agcgcctgtc gcttctgcaa cgttattatc acccagaaag attgcaacgt
    57961 gactcacaga ggaagtgctg aagacgcgga ttccaaatga ggttacccca aggcttgagg
    58021 agaacagcaa atcgccgggt ttgagatcag gtgccgttat ttcttttatt gattgttcgg
    58081 tgaaagagct ttgatgctgg aatttgacag cccatgtttt tgcctcggca tctactgctg
    58141 tggctgatgg gtctggctgg ctgatatcaa cggtacaagc cgaaagtaaa agaaagctgg
    58201 ggagaagcag gcggcagtac gcctttggtt tatccatttt atacaatcca tgtaaaaaag
    58261 ggccctgaaa ttcaggaccc tttctggcat cagcctttaa tctgtttcac cagatattcg
    58321 acgatgtcac cagtcttaat taactgtttc tcgccgttgc gacgatattt atattcgata
    58381 tcgtcgttgt cgaggttacg gtcgcccagc acaatagtgt gcggaatacc gatcagttcc
    58441 atatcagcaa acatcacgcc cggacgctct ttgcggtcat ccagcagcac ttcgatacct
    58501 tgtgcacgca gttcgctgta cagtttctca gcaagctctt gtacgcggaa ggatttgtgc
    58561 atgttcatcg gcagaatcgc cacctggaac ggcgcgatag cgtcaggcca tacgatgccg
    58621 cgttcgtcgt agttctgctc aatcgccgca gctaccacac gcgttacccc gataccgtag
    58681 caacccatcg tcaggatttg gttacggcca tcttcaccct gtacggaggc tttcagtgct
    58741 tcggagtact tggtacccag ctggaagatg tgaccaactt cgataccacg tttgatcagc
    58801 agcgtaccct ggccatccgg gcttggatcg ccagccacca cgttacggat atctgcaact
    58861 tccggggtag cgacatcgcg atcccagttg ataccgaagt agtgtttacc atcgatgtta
    58921 gcaccagcag cgaaatcact catcgccgca acggtacggt caatcaccac cggaatcggc
    58981 atgtttaccg gacccagtga acccggaccg gctttaacca cagcacgaat ttcttcttcg
    59041 gtcgcgaaag tcagcgggct tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc
    59101 tcgtgatcac cgcgcaccag cagcgcaacc agcgggaagc tactgccttc aaccgcttta
    59161 atcagcagag tcttaaccgt tttctcaatc ggcagattga actgttcaac cagttccgcg
    59221 atggttttcg cgttcggcgt atcaaccagc gtcatttcct gggtagcagc agcgcgcggt
    59281 tctttcggcg cgatagcttc tgccagttca atgttcgctg catagtcaga ggtgtcggag
    59341 aagaccacat cgtcttcacc gctctgcgcc agcacctgga attcgtgaga ggcgctgccg
    59401 ccgatagaac cggtgtcggc ctgtacggcg cggaaatcca gccccatgcg gctgaagatt
    59461 ttgctgtagg ccgcatacat tgcatcgtag gtttcctgca gggattcctg agaagtatgg
    59521 aaagagtaag catctttcat caggaattcg cgggaacgca tgacgccgaa acgcggacgc
    59581 acttcgtcgc ggaacttggt ctggatctga tagaagttca gcggcagctg tttgtaagag
    59641 ctaagctcgt tacgaatcag gtcagtgata acttcttcat gagttgggcc gagtacgaac
    59701 ggacgctcgc cacggtcaac aaaacgcagc agttccggac cgtactgttc ccaacgacca
    59761 ctctcttgcc acaaatcggc tggctgaacc accggcatcg acacctcgat cgcaccggcg
    59821 ttgttcatct cttcacgcac gatgttttcg acttttttca gaacgcgcac gccggtcggc
    59881 agccaggtat ataacccgga ggccagcttg cggatcatcc cggcgcgcag catcagctga
    59941 tggctgatca cctcggcgtc ggcaggtgtc tccttgagag tggagagcag gtattggcta
    60001 gtacgcatgt tgttacggtt ccagttggaa ggtagaacag gctcaaggcg agcctgggac
    60061 aaaaaaagtg atttagttta ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt
    60121 aacgcggttc cagcgcaaag acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa
    60181 aatcatgtag ccagacggca taggttttgc ccgtttcctc acctttacga taggccgggc
    60241 gcgggtcctg cgccagtact tcgcggataa acagcgttaa ctgcggataa cgcttctcca
    60301 gcgtcaaaag ctgcttttcg acctctgcgg taaaactcac cgccatctct gcagctggcg
    60361 cactttgcgc atagctggca ctggcatcgg gaagcgattc ggcaaagggg agatacggtt
    60421 tgatatccac taccggcgta ccatcgacca gatccagact gccgagcttc agaatcacgc
    60481 tgcctttatg gcaaacaacc tctttcagct ctaccagcga catgccaatt gggttagggc
    60541 ggaaagtaga gcgtgttgcg aaaaccccca ttctggcgtt accgccgagg cgcggtggac
    60601 gcacagtcgg acgccagccg ccttccatcg tttgatgaaa gacgaaaagg atccataaat
    60661 ggctgaatgc ttccaggccg cgaacggcgt cggcctggtt gtaggaagca atgagatgca
    60721 gttcgccgtt ggcgcttttt accagacctg gctggcgcgg aacggcgaat ttttctttat
    60781 agggcgagcg aataacgcct atttgctcga actggaaact gctcatttcg ccgtaatgtt
    60841 aagcgcagag ccgatacata cagcctgacg atagcagccc ggcgtaccgc tggtgacttc
    60901 gcagctatgc agtaatacag cattggcttt cattttagag gcattgattt gcatctgctt
    60961 acgtgcggtt ggaatgctcg gcggagagtc ctgattagag gcctggcaag agtcgccact
    61021 gacttcaccg agatcgcgga acggtttgcc gactaattct tctgcattgg tataaattcg
    61081 gaccggcgtg gcgcgcggcg ctttcggttt tgcaggctcc gctttcggct ggggtgcagt
    61141 gctttgaacg ggttcgacag gggatctgct taacatggaa cagccgctta gcatgagtgc
    61201 tactaaacag atcggtaaag cacgcatagt atttcctcaa tgtatgatca aaacgtcaat
    61261 attgaatcag gagcttgtaa aaatgacaag acgggcaagc gcccgtcctg aatgatatta
    61321 caaattgtgg aaacagccta aaaattacca gcctttaaca gcgccgccgt taaacacttt
    61381 gtttgctgct tcgtaaactt catcagactg ataagcctgg acgaatttct tcacgttctc
    61441 ggcgtctttg ttatcttcac gcgttacgat caggtttacg tatggggagt ctttatcttc
    61501 gacaaagata ccgtctttcg ccggagtcag gccaatctgg ctggcatagg tggtattgat
    61561 aactgccaga gcgatttgcg cgtcgtccag agaacgcggc agttgcggtg cttccagttc
    61621 aacaattttc agattttttg ggttctcaac aacatcaaga acggtcggca gcaggccaac
    61681 gccatctttc agtttgatca agcccacttt ttgcagcagc agcagtgaac gaccaagatt
    61741 agttggatcg tttggcacgg caacctgcga accatcctgc agttcatcca gtgatttgat
    61801 tttcttggag taaccagcaa tcggataaac aaaagtgttg cctactgcga tcagtttgta
    61861 gccacgatct ttcagttgct gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat
    61921 atcgcctttg ctcaatgctt cgtttggcag aacatagtcg ttgaaggtta ccagctcaac
    61981 gtccaggcca tatttgtctt tcgcaacttt ctgcgcgact tctgctacct gctgttcggc
    62041 accaacaatc acgccgactt taatgtggtt tggatctttt tcatcctgac cgcagcctac
    62101 cagtgccagt gatccaatca gggctcccac tgccgcaaag gttttgaatt tgaacgccat
    62161 accttattcc ttcttcaatt atttatgttg tgttgaacgt tacttgcgag tgacagcccg
    62221 gacgatgcgg tcgcctgcga actgaattaa ataaaccaga atgaccagca ataccagtac
    62281 cgtattcatc accgtcgcgt tgtagccgat gtagccatac tgatagccaa tctgacctaa
    62341 accaccggca ccgactgcac cacccatcgc ggaataaccg accagggtaa tcagggtgat
    62401 agttgccgca ttcaccagac ccggcaacgc ttccggtaac agcactttac agacgatctg
    62461 cattggcgtg gcacccattg cgcgggaagc ttcaattaac ccggttggga tctccagcag
    62521 agcgttctcg accatacggg caataaacgg tgctgcacca acggttaacg gaacaattgc
    62581 tgcctgcaaa ccaatcgatg taccgacaat aacgcgggta aacggaatca tccataccag
    62641 caagataatg aacgggatgg aacggaaaat gttcacaatc gcagaaacgg tacgatacag
    62701 cttcgcgtta gcaataattt gccccggacg cgtgacataa agcagaacgc caaccggcag
    62761 accaatcaca aacccaaaaa atccggatac gaaggtcatt gccagcgttt cccatacgcc
    62821 acgaaccagc agccacatca tcggctcaga cataacccag tacctctact tttacatggt
    62881 gttcctgcag ccaggcaatg gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca
    62941 gcatgatgcc gaatttaacg ccaccggcgt agtccatctg cgcgctaata atgttgttgt
    63001 tgacgttgaa acgacgcgcg gtttcagaaa gcagtggggc atcgaccgat tgaccggtaa
    63061 actccagacg cagcatcggc acgcagtcag taaatggctc cgtttgcaga cgttcctggt
    63121 aatcttccgg gatatccaga tgcagggtcg actgaataaa cttctgcgcc agcggcgttt
    63181 tcggatgcga gaacacttca cttaccgtgt cctgctcgat cagttcgcca ttgctgatga
    63241 ccgccacgca atcacaaata cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg
    63301 tcaaacccag acggcggtta atgtctttca gcagttcgag aatagaacgt gtcgttgccg
    63361 ggtccagcgc gctggtggct tcatcacaca gcaatacttt gggattgctg gctaatgcac
    63421 gggcaatcgc cacacgttgt ttctgcccac cggaaagatt cgacgggtag ctatcatgct
    63481 tatcgccaag accaaccaat gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg
    63541 gtgtgttgtc cagctccagc ggcagagcca cgttgccaaa aacagtacgc gaagagagca
    63601 ggttaaaatg ctggaaaatc ataccaatct ggcggcgagc tttggtcaac tcggattctg
    63661 acagcgtggt caattcctgg ccatcgacca gcacgctacc ctcggttggg cgctccagca
    63721 ggtttacaca acgtataagc gtactcttac ccgcgcctga ggcaccgata acgccataaa
    63781 tttgtccagc tggcacatgc aggctgacgt tgttcaacgc ctggatggtg cgggtgccct
    63841 ggtggaacac tttggtgata ttcgaaagtt ttatcattga ttatttatta tcgtcattaa
    63901 gttagtcgta gcatctcgaa tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa
    63961 ggcatccaga cgtctaaatc aatcaggttt atgcgaagag cactttcttg caggtcgaaa
    64021 catgcgatac tagcgtccca tgccttatta aggagctata aaaggtggcg aagagcgtac
    64081 ccgcaatttt tcttgaccgt gatggcacca ttaatgtcga tcacggctat gtccatgaga
    64141 tcgacaactt tgaatttatc gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg
    64201 gctttgcgct ggtggtagta accaaccagt ctggcattgc tcgcggtaaa tttaccgaag
    64261 cacagtttga aacgctgacc gagtggatgg actggtcgct ggcggaccga gatgtcgatc
    64321 tggatggtat ctattattgc ccgcatcatc cgcaggggag tgttgaagag tttcgccagg
    64381 tctgcgattg ccgcaaacca catccgggga tgcttttgtc agcacgcgat tatttgcata
    64441 ttgatatggc cgcttcttat atggtgggcg ataaattaga agatatgcag gcagcggttg
    64501 cggcgaacgt gggaacaaaa gtgctggtgc gtacgggtaa acctattaca cctgaagcag
    64561 aaaacgcggc agattgggtg ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc
    64621 agcaaaaacc ggcgcaatga ttaaaagatg aacggttgaa ataaaaatgc atttttccgc
    64681 ttgtcttcct gagccgactc cctataatgc gcctccatcg acacggcgga tgtgaatcac
    64741 ttcacacaaa cagccggttc ggttgaagag aaaaaatcct gaaattcagg gttgactctg
    64801 aaagaggaaa gtgtgatata cgccacctcg cgacagtgag ctgaaagctg cgtcgcaact
    64861 gctctttaac aatttatcag acaatctgtg tgggcactcg aagatacgga ttcttaacgt
    64921 cgcaagacga
//
LOCUS       DAJVPP010000008        63250 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVPP010000008 DAJVPP010000000
VERSION     DAJVPP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.008"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..2353)
                     /gene="glgP"
                     /locus_tag="OQM34_000523"
     CDS             complement(<1..2353)
                     /gene="glgP"
                     /locus_tag="OQM34_000523"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1451095.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKA"
     gene            complement(2372..3805)
                     /gene="glgA"
                     /locus_tag="OQM34_000524"
     CDS             complement(2372..3805)
                     /gene="glgA"
                     /locus_tag="OQM34_000524"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1451096.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3805..5100)
                     /gene="glgC"
                     /locus_tag="OQM34_000525"
     CDS             complement(3805..5100)
                     /gene="glgC"
                     /locus_tag="OQM34_000525"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1451097.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5118..7091)
                     /gene="glgX"
                     /locus_tag="OQM34_000526"
     CDS             complement(5118..7091)
                     /gene="glgX"
                     /locus_tag="OQM34_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1451098.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPV
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7088..9274)
                     /gene="glgB"
                     /locus_tag="OQM34_000527"
     CDS             complement(7088..9274)
                     /gene="glgB"
                     /locus_tag="OQM34_000527"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1451099.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQEWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9547..10650)
                     /gene="asd"
                     /locus_tag="OQM34_000528"
     CDS             complement(9547..10650)
                     /gene="asd"
                     /locus_tag="OQM34_000528"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1451100.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10842..11435
                     /gene="yhgN"
                     /locus_tag="OQM34_000529"
     CDS             10842..11435
                     /gene="yhgN"
                     /locus_tag="OQM34_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1451101.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11492..12832)
                     /gene="gntU"
                     /locus_tag="OQM34_000530"
     CDS             complement(11492..12832)
                     /gene="gntU"
                     /locus_tag="OQM34_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1451102.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12836..13363)
                     /gene="gntK"
                     /locus_tag="OQM34_000531"
     CDS             complement(12836..13363)
                     /gene="gntK"
                     /locus_tag="OQM34_000531"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1451103.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13502..14497)
                     /gene="gntR"
                     /locus_tag="OQM34_000532"
     CDS             complement(13502..14497)
                     /gene="gntR"
                     /locus_tag="OQM34_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1451104.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14721..15416)
                     /gene="yhhW"
                     /locus_tag="OQM34_000533"
     CDS             complement(14721..15416)
                     /gene="yhhW"
                     /locus_tag="OQM34_000533"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1451105.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15539..16576)
                     /locus_tag="OQM34_000534"
     CDS             complement(15539..16576)
                     /locus_tag="OQM34_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1451106.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            16909..17397
                     /gene="yhhY"
                     /locus_tag="OQM34_000535"
     CDS             16909..17397
                     /gene="yhhY"
                     /locus_tag="OQM34_000535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1451107.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17456..19198)
                     /gene="ggt"
                     /locus_tag="OQM34_000536"
     CDS             complement(17456..19198)
                     /gene="ggt"
                     /locus_tag="OQM34_000536"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS1451108.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19318..19758
                     /locus_tag="OQM34_000537"
     CDS             19318..19758
                     /locus_tag="OQM34_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS1451109.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19745..20488)
                     /gene="ugpQ"
                     /locus_tag="OQM34_000538"
     CDS             complement(19745..20488)
                     /gene="ugpQ"
                     /locus_tag="OQM34_000538"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1451110.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAG
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20485..21475)
                     /locus_tag="OQM34_000539"
                     /pseudo
     CDS             complement(20485..21475)
                     /locus_tag="OQM34_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21477..22322)
                     /gene="ugpE"
                     /locus_tag="OQM34_000540"
     CDS             complement(21477..22322)
                     /gene="ugpE"
                     /locus_tag="OQM34_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS1451111.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22319..23206)
                     /gene="ugpA"
                     /locus_tag="OQM34_000541"
     CDS             complement(22319..23206)
                     /gene="ugpA"
                     /locus_tag="OQM34_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCS1451112.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23304..24620)
                     /gene="ugpB"
                     /locus_tag="OQM34_000542"
     CDS             complement(23304..24620)
                     /gene="ugpB"
                     /locus_tag="OQM34_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCS1451113.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25017..25730)
                     /gene="livF"
                     /locus_tag="OQM34_000543"
     CDS             complement(25017..25730)
                     /gene="livF"
                     /locus_tag="OQM34_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS1451114.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25732..26499)
                     /gene="livG"
                     /locus_tag="OQM34_000544"
     CDS             complement(25732..26499)
                     /gene="livG"
                     /locus_tag="OQM34_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS1451115.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26496..27773)
                     /gene="livM"
                     /locus_tag="OQM34_000545"
     CDS             complement(26496..27773)
                     /gene="livM"
                     /locus_tag="OQM34_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS1451116.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27770..28696)
                     /gene="livH"
                     /locus_tag="OQM34_000546"
     CDS             complement(27770..28696)
                     /gene="livH"
                     /locus_tag="OQM34_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS1451117.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28744..29853)
                     /gene="livK"
                     /locus_tag="OQM34_000547"
     CDS             complement(28744..29853)
                     /gene="livK"
                     /locus_tag="OQM34_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS1451118.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30277..30660
                     /gene="panM"
                     /locus_tag="OQM34_000548"
     CDS             30277..30660
                     /gene="panM"
                     /locus_tag="OQM34_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1451119.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30859..31962)
                     /gene="livJ"
                     /locus_tag="OQM34_000549"
     CDS             complement(30859..31962)
                     /gene="livJ"
                     /locus_tag="OQM34_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1451120.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32233..33087)
                     /gene="rpoH"
                     /locus_tag="OQM34_000550"
     CDS             complement(32233..33087)
                     /gene="rpoH"
                     /locus_tag="OQM34_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1451121.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33332..34390)
                     /gene="ftsX"
                     /locus_tag="OQM34_000551"
     CDS             complement(33332..34390)
                     /gene="ftsX"
                     /locus_tag="OQM34_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1451122.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQVA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34383..35051)
                     /gene="ftsE"
                     /locus_tag="OQM34_000552"
     CDS             complement(34383..35051)
                     /gene="ftsE"
                     /locus_tag="OQM34_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1451123.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35054..36550)
                     /gene="ftsY"
                     /locus_tag="OQM34_000553"
     CDS             complement(35054..36550)
                     /gene="ftsY"
                     /locus_tag="OQM34_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1451124.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36700..37296
                     /gene="rsmD"
                     /locus_tag="OQM34_000554"
     CDS             36700..37296
                     /gene="rsmD"
                     /locus_tag="OQM34_000554"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1451125.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37286..37555
                     /locus_tag="OQM34_000555"
     CDS             37286..37555
                     /locus_tag="OQM34_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1451126.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37558..37917)
                     /locus_tag="OQM34_000556"
     CDS             complement(37558..37917)
                     /locus_tag="OQM34_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1451127.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38058..38684
                     /locus_tag="OQM34_000557"
     CDS             38058..38684
                     /locus_tag="OQM34_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1451128.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38758..40956
                     /gene="zntA"
                     /locus_tag="OQM34_000558"
     CDS             38758..40956
                     /gene="zntA"
                     /locus_tag="OQM34_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1451129.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSPPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41058..41303)
                     /gene="tusA"
                     /locus_tag="OQM34_000559"
     CDS             complement(41058..41303)
                     /gene="tusA"
                     /locus_tag="OQM34_000559"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1451130.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41523..42188
                     /locus_tag="OQM34_000560"
     CDS             41523..42188
                     /locus_tag="OQM34_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1451131.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42261..42818
                     /gene="dcrB"
                     /locus_tag="OQM34_000561"
     CDS             42261..42818
                     /gene="dcrB"
                     /locus_tag="OQM34_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1451132.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42822..44039)
                     /locus_tag="OQM34_000562"
     CDS             complement(42822..44039)
                     /locus_tag="OQM34_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1451133.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44173..45222
                     /locus_tag="OQM34_000563"
     CDS             44173..45222
                     /locus_tag="OQM34_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1451134.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45277..45864
                     /gene="acpT"
                     /locus_tag="OQM34_000564"
     CDS             45277..45864
                     /gene="acpT"
                     /locus_tag="OQM34_000564"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1451135.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            45975..47549
                     /gene="nikA"
                     /locus_tag="OQM34_000565"
     CDS             45975..47549
                     /gene="nikA"
                     /locus_tag="OQM34_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1451136.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVPATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47549..48493
                     /gene="nikB"
                     /locus_tag="OQM34_000566"
     CDS             47549..48493
                     /gene="nikB"
                     /locus_tag="OQM34_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1451137.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48490..49323
                     /gene="nikC"
                     /locus_tag="OQM34_000567"
     CDS             48490..49323
                     /gene="nikC"
                     /locus_tag="OQM34_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1451138.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49323..50087
                     /gene="nikD"
                     /locus_tag="OQM34_000568"
     CDS             49323..50087
                     /gene="nikD"
                     /locus_tag="OQM34_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1451139.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50084..50890
                     /gene="nikE"
                     /locus_tag="OQM34_000569"
     CDS             50084..50890
                     /gene="nikE"
                     /locus_tag="OQM34_000569"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1451140.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            50896..51297
                     /gene="nikR"
                     /locus_tag="OQM34_000570"
     CDS             50896..51297
                     /gene="nikR"
                     /locus_tag="OQM34_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1451141.1"
                     /translation="MQRVTITLDDDLLKTLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51417..51776)
                     /locus_tag="OQM34_000571"
     CDS             complement(51417..51776)
                     /locus_tag="OQM34_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1451142.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYVRTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51773..52048)
                     /locus_tag="OQM34_000572"
     CDS             complement(51773..52048)
                     /locus_tag="OQM34_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCS1451143.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52107..53231)
                     /locus_tag="OQM34_000573"
     CDS             complement(52107..53231)
                     /locus_tag="OQM34_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1451144.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53231..55966)
                     /gene="rbbA"
                     /locus_tag="OQM34_000574"
     CDS             complement(53231..55966)
                     /gene="rbbA"
                     /locus_tag="OQM34_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1451145.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(55963..57030)
                     /locus_tag="OQM34_000575"
     CDS             complement(55963..57030)
                     /locus_tag="OQM34_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1451146.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57396..59018)
                     /locus_tag="OQM34_000576"
     CDS             complement(57396..59018)
                     /locus_tag="OQM34_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1451147.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59280..60869)
                     /locus_tag="OQM34_000577"
     CDS             complement(59280..60869)
                     /locus_tag="OQM34_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1451148.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61253..62284
                     /locus_tag="OQM34_000578"
                     /pseudo
     CDS             61253..62284
                     /locus_tag="OQM34_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62371..62847)
                     /locus_tag="OQM34_000579"
     CDS             complement(62371..62847)
                     /locus_tag="OQM34_000579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451149.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62840..>63250)
                     /locus_tag="OQM34_000580"
     CDS             complement(62840..>63250)
                     /locus_tag="OQM34_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1451150.1"
                     /translation="SHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKDTY
                     SITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDVNS
                     MHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT        15173 a        17141 c        15739 g        15197 t
ORIGIN      
        1 tcgcttttgc ggtccactct tcctgaagct cgtagagttc gtcgacttta tcctgacaat
       61 cgacatagct gcgataatcc gccagtacct ggtagtgatc gccgaagttg atcagcgaat
      121 caaccagatc gcgatagcga cccggatctt ccggactgaa tacaccgctg ccgatttgcg
      181 tcagcacctg atgcagctcc tcatctttct cgtagtattc acgcggtttg tagccctgac
      241 gacgcagttc ttccacttct tccgctgtgt taccaaaaat aaagatattg tcagcaccga
      301 catgatccag catctcgaca ttcgcaccgt ccaacgtacc gatagtcagc gcaccgttaa
      361 gcgcaaactt catgttactg gtgccggaag cttccgtccc tgccagcgaa atctgttcag
      421 acagatctgc cgccggaatg atcaactgcg ccaggctaac gctgtagttc gggatgaaca
      481 cgactttcag cttatcgcca atctgcggat cgttgttgat cactttcgct acgtcattga
      541 tcaaatgaat aatgtgcttc gccatgtaat aggccgaagc cgccttaccg ccaaaaatat
      601 tcacgcgcgg tacccacttc gcatccgggt cggccttgat gcggttatag cgggtaatca
      661 catgcaacac attcatcaat tgacgtttgt attcgtgaat acgtttgatt tgtacatcga
      721 acaacgcctt tggattcacc accacattca gctgctgggc gatatactct gccagacgct
      781 ttttgttctc cagcttcgcc tgatgcacag cgtgattaac cattgggaaa tcacagtgtt
      841 gttgcagctc attaagcagg ctaaggtcgg tgcgccagtt acggcccagg tgttcgtcca
      901 gcacggctga aagcgatggg ttcgctaccg ccagccagcg acgcggcgtc acaccgttgg
      961 tgacgttggt gaaacgaccc gggaagattt tcgcaaagtc ggcaaacaac gattgcacca
     1021 tcagattaga gtgcagttcc gatacaccgt taactttgtg gctcacaaca accgccagcc
     1081 aggccatacg cacacgacga ccgttggatt catcaatgat cgacgcccgt cccagcagat
     1141 cggtatcgtt cggatactgt tcctgcaagg ttttcaggaa atagtcgttg atttcaaaga
     1201 tgatctgcag gtgacgcggc agaattttac ccagcatatc aaccggccag gtttccagcg
     1261 cctcgctcat cagcgtgtgg ttagtgtagg agaagacctg acaacacacc tcaaacgcgt
     1321 cgtcccagct aaattggtgc tcatcgatca gcagacgcat catctcagga atcgacagta
     1381 ccggatgggt atcattgaga tgaatcgcga ttttatccgc caggttatcg taggttttat
     1441 gcaactgata atgacggctt aaaatgtcct gaatggtcga ggaaaccaag aagtattcct
     1501 gacgcaggcg cagctcacgc ccggagtagg tggagtcatc cggatacagt acgcgagata
     1561 cgttctcgga gtggttttta tcttccactg ccgcgaagta gtcaccctgg ttgaatttac
     1621 cgaggttaat ttcgctactg gcttgcgcac tccacaaacg cagcgtgttg gtcgcgtcgg
     1681 tgtcgtaacc agggattatc tgatcgtaag cgactcccag aatctcttcg gtttcaatcc
     1741 agcgcgtttt tttaccttcc tgctgaatgc gaccaccaaa acggacttta tagcgcgtgt
     1801 tgtggcgttt gaattcccac gggttaccgt attccagcca gtagtctggc gactctttct
     1861 ggctaccgtt aacgatgttc tgcttgaaca taccgtagtc atagcggatg ccgtaaccgc
     1921 gccccggcaa ccctaacgtc gccagagaat caaggaagca agccgccaga cgtcccaggc
     1981 caccgttacc gaggcctggg tcattttctt catcaatcag ctcttcgaga tttaacccca
     2041 tcgcttccag tgcgccctgt acatcttcgt aaattcctag cgacaacatg gcgttggaga
     2101 gcgtacggcc aatcaaaaac tccatcgaca ggtagtaaac ctgacgagtt tcttgcgaca
     2161 actgggcacg gtttgaacgt aaccagcgct ccacgagacg atcgcgcaca gcaaataacg
     2221 ttgcgttcag ccattcatgt ttattggcga cgaccgggtc ctttccaatc gtaaacatca
     2281 gcttgtaagc gatagagtgc ttaagagctt ctacgctaag cgtgggcgat gaatatgtaa
     2341 acggagcatt catataggcg tttcctgaaa actatttcaa gcgatagtaa agctcacggt
     2401 acgacttcgc cgcgacctgc cagctaaaat ccattgccat agcctgacgt tgcacaaacc
     2461 gccacagtga aggacgggac cacagtacaa aagcacgccg aatagcccgt aacagcgacc
     2521 aggcattact atcttcaaag acaaacccac tggcgacgcc atctgcaagg ttctcaagag
     2581 aacagtcaga aaccgtatca gcaagcccac cggtgcgccg cactaacggc agcgtaccgt
     2641 acttcaatcc ataaagttgc gttaagccgc acggttcaaa acggctgggc accagaatga
     2701 cgtccgcgcc gcccataatg cgatgcgaaa atgcttcgtg atagccaatc tgaacgccca
     2761 cctgaccggg gtattccgct gccgccgcaa ggaaaccttc ctgcagcacc ggatcgcccg
     2821 cgccgagtag cgccagctgc ccgccctgct ccagaagccc cggtaaggct tccagcacca
     2881 ggtcgagacc tttctggctg gtcagacggc tcaccactgc aaaaagcggc actttatcgt
     2941 caaccttaag ccccattgcg atttgtaact ggctcttatt ttccgcttta tcttccaacg
     3001 tatcgcgggt gtaacgcgag gccaacagta agtccgtctc tggactccag attttctcgt
     3061 ccacgccgtt cagtacgcct gaaagacgcc cttcacggtg acgctgttgc aacagacctt
     3121 ccataccgta ggcaaactgc ggttcggtga tctcgcgagc gtaggttgga ctgaccgccg
     3181 taatgtgatc ggcatagtac agaccggcct tcaggaaaga gatttgtccg ttgaattcca
     3241 gcccatgaat attaaagaat gaccatggca attggatgtc attcatgtga tgtgcataaa
     3301 acatgccttg ataggccagg ttgtgcacag taaacaccga cttcgcagga cgcccgcgcg
     3361 ccgccagata cgcaggcgca agacctgcat gccagtcgtg cgcatgcacc acatcaggac
     3421 gccagaatgg gtcaagcccg ctggccattt ctgccccaac ccaccccagc agcgcaaaac
     3481 gcaatacgtt gtcggtatag gcaaataagt tggtatcgtg atacgggctt cccggacgat
     3541 catagagatg cggcgcgtca atcaggtata tgccaacccc gttgtaatga ccgaacaaca
     3601 gcgtgatatg cccggcgaaa gtatcacgac gggatactac ctgcgcatcg gtcacgccac
     3661 ggcgaatatc gggaaatgca ggcaacagta cgcgagcgtc aacgccgtct gcgatttgtg
     3721 ctgcgggtaa tgccccaata acatcagcca gaccgccggt tttaagcagc gggaacatct
     3781 ctgaacatac atgtaaaacc tgcattatcg ctcctgttta tgccctaact tccgtagcat
     3841 ttcgcgcgtt accagcacga tgccttcttc tgaacgatag aaacgacgtg catcttcctc
     3901 tgcgttttca ccaatcacca tgccttccgg gataacacaa gcacgatcga tgacgcagcg
     3961 gcgcagacgg cacgagcgac ctacccatac ttccggtaac aatacggcgg aatcaatgtt
     4021 gcagaatgaa ttcacgcgaa cgcgcgagaa cagaacggac tgcaccacca ccgaaccgga
     4081 gatcacacaa ccgccggata ccagtgagtt aagggtcatc ccgtggctac cggagcgatc
     4141 ctgcacgaat ttcgctggcg gtaatgattc attgtaggtg cgaattggcc aattgcgatc
     4201 gtacatatcc agttccggca ccacagaggc cagatcgaga ttcgctttcc agtaagcttc
     4261 cagcgtaccc acatcgcgcc agtacggctc ggcatccggg tcggattgta cgcaagagag
     4321 cgggaacggg tgcgcatagg ccagaccggc ttcggtgatc ttgggaatca aatctttgcc
     4381 aaagtcgtgg ctggagttct catcacgatc gtcttcttcc agcagttcat acagatagtc
     4441 ggcgtcaaag acgtagatac ccatactcgc cagagatttg ctcggatcgt tcggcattga
     4501 cggtgggtta gcaggttttt caacgaattc gataatttta tcgttctcat caaccgccat
     4561 aacgccaaat gcggaggctt cttcaatcgg tactggcata caagcaacgg tgcaacgtgc
     4621 gcctttttcg acgtgatcga taagcatacg cgagtagtct tgcttgtaga tatggtcgcc
     4681 cgccaggatc accacgtatt ccgctttata acggcggata atgtcgaggt tttgggtgac
     4741 cgcatctgcg gtgccgcgat accagttttc ccctttcatt ctctgctgtg ctggcagcag
     4801 atcgacaaac tcgttcattt cttcattgaa gaatgaccag ccgcgctgaa tgtgctgcac
     4861 cagagtgtgg gactggtact gggtgatcac gcccatacga cggatcccag agttgatgca
     4921 gttagacagc gcaaagtcaa taatgcggaa cttaccgccg aagtgtacgg ccggttttgc
     4981 tcgcttattg gttaaatcct tcaggcgggt accacgtcct cccgccagta tcagggcaac
     5041 agatttcaat ggcagctggc gcgccaacat taagtgatcg ttcttctcta aactaaccat
     5101 gactaactcc ttttttatca tctctggaac acacacaatc cgtgtgcagg tccctgccag
     5161 acaaccgtaa tcactgggtt atcctctcca gcgaatgggg gaatggcgtg ccactcccca
     5221 acaggtaaaa caatctctgt tacctcaagc gtggcgttaa ttgcgatcaa aaagcgatcc
     5281 gagagcagaa tttgcagctg tttcggcccg ttttgccact catccgtgct taaaggttga
     5341 gcatatcgat ttagccaacg gacattgccg tcaccttctt cccaccagcg attctccacc
     5401 aaagcgggaa tgcgcttgcg cagatggatt aacgcggcgg taaatgcggt taaaccactg
     5461 cttgcctgcg accagtccaa ccagtttaat tggttatcct gacagtaggc attgttattg
     5521 ccatgctggc tgtgaccatg ttcgtcaccg gccagtaaca tcggcgtgcc ctgggagagc
     5581 aacaacgttg ttaacagggc atgaatgctg tcgcgccgcc gttcaaccag gtcaagagta
     5641 ccgcctaacc cttctttacc atgattgtta ctgtaattgt tgttggtccc gtcgcgattt
     5701 tcttctccgt ttgcttcatt gtgtttatgg ttgaagcaaa cgcagtcgcg aagcgtaaaa
     5761 ccgtcatgcg cggtgacgag attaatcgcg gcactcggca gacgaccatt acgtttaaaa
     5821 acatcgctgg aggcagcaaa acgcccggca aacgccccca gaggcaaatc ataatgtagc
     5881 cagaaacgac gggcagcatc gcggaaatga tcgttccact cggcaaacag cggcgggaaa
     5941 tttcccacct gataaccact aggagcgata tcccacggtt cagcaattaa cttcacctgc
     6001 gagagcaccg ggcagttctg gatagcggta aacaacggcg catcctgacg gaactctggc
     6061 gtacggccca tgactgccgc cagatcaaag cggaaaccat cgacgtggca ggtttctacc
     6121 caataacgca ggcaggcgct ggcataatcc accaccgccg gatgactcaa attgagcgtg
     6181 ttgccgcaac cggtccagtt gtgataatcg ccgtcttctc ttatccaata atagctacgg
     6241 ttatcgatcc cacgcagcga gaataacggg ccgtcgaggt ccagttccgc actatggttg
     6301 agcacgatat caagaatgac ttcgataccc gctttatgca gtgctttgat tgcatcgcga
     6361 aactcatcca gcgccgtttc tggcgagcag gcatacgccg gatgcagcgc aaacatcgcc
     6421 accgggttgt aaccccagta gttacttagc cccatgcgtt gcagacgtgg ttcactggca
     6481 aactgcgcca ctggcagcag ttccagcgcg gtaatgccca attgtttcaa atagttgatc
     6541 atcaccggat gcccgagggc tttataagtg ccacggatct cgaccgggat ctccgggtgc
     6601 aagtacgtta atcctttgac atgggcttca taaatgatgg tgctgcccca cggcgtgcgc
     6661 ggcggggcgt catcttccca gtcatagtga tcaaccacca ctacgcattt cggcgcaatg
     6721 gcggcgttgt cgcgatagtc aggttcatta tgaccggcgt gcagcagcgg gttatcttta
     6781 aactccccgt caatttgccg cgcgcaagga tcaatcaaca acttcgccgg gttaaagcga
     6841 tgcccctcgg cgggttgcca ggggccatga acgcgataac cataacgcaa acccgggcgc
     6901 gcatccggca gataaccgtg ccaaatgtcg ccactgttcc ctggcaagtc atagcgatgt
     6961 tcctggccat tggcgtcaaa gacacacagt tctacccgct cggcatgagc ggagaaaagt
     7021 gtgaagttga cgccctgacc gtcgtaatgc gcgccgaggg gagcgggttt gccaatggcg
     7081 agttgtgtca ttctgcctcc cgaaccagcc agatagtggc cagcggtggt agcgtcaggc
     7141 ttagtgaatg ctgacgaccg tggctggcaa tctcatcgct gtgtaccgtg ccgccattgc
     7201 ctgcattact gccgtgatag tgtatggaat cggtattgag gatttcacgc catttacctg
     7261 gctgattaat gcagaagcga taatcatgac gcggcaccgg cgtaaagtta ctggcaacga
     7321 taatttcgtt accctcttta tcgcgacgca caaagatcag caccgagcgt tctttgtcat
     7381 ccaccaccag ccattcaaag ccgtacgggt caaaatccag ttcatgcatt gctttatggt
     7441 ggcggtaggt gaggttcaga tcgcgcacca gacgctggac accgtggtgc cagttatcgc
     7501 cgccttccaa cagatgccag tcgaggctgg cgtcatggtt ccactcgcgg ccctgggcaa
     7561 attcgttccc catgaacagc agtttcttgc ccggaaatgc ccacatccag ccatagtaag
     7621 cgcgcaggtt agcgaatttc tgccatgcgt cgcccggcat gcggtcgaga atcgattttt
     7681 taccgtggac cacttcatca tgcgacaacg gcaggacgaa gttttcagtg tagttgtaga
     7741 gcatcccgaa ggtcagttta tcgtgatgat actgacgata aatcgggtcg agcttcatgt
     7801 agtccagggt gtcatgcatc cagccgaggt tccacttgta ccagaagccc agaccgccca
     7861 tatcctgcgg acgagaaacg ccagggaaat cggtagactc ctcagccatt gtcaccgcac
     7921 cggaaacctg ctcaccaaga atacggttgg tattacgcaa gaattcaatc gcttcaagat
     7981 tctcgcgccc gccaaattcg ttcgggatcc actccccctc tttacggctg tagtcacgat
     8041 aaatcatcga cgccaccgca tcgacgcgca gcgcatcaat accaaaacgt tcaatccagt
     8101 aaagcgcgtt accgacgagg aagttactga cttcacggcg accatagttg tagatcagcg
     8161 tgttccattc ctgatgatag ccttcacgcg gatcgctgtg ttcatacaag ttcgtgccat
     8221 caaattcggc aagcgcaaag tcatcagtcg ggaagtggcc tggcacccag tcgagaatca
     8281 cgttcagacc agctgcgtgt gcggcatcaa tgaaataacg gaagtcgtcg cgagtaccaa
     8341 aacggcgggt tggtgcatac aggccggttg gctgataacc ccaactgcca tcgaagggat
     8401 gctcgttaat gggcagtagt tcgaggtggg taaagcccat ccatttagca taaggcacca
     8461 gttgatcggc cagctcgcgg tagctcaacc agaaattgtt gtcggtgtgg cgacgccagg
     8521 aacccaggtg aacttcataa atagagattg gcgcatcaaa ctgattcgct tttttgcgct
     8581 cttcagtctg tacaaccttt tccggcagcc cgcaaataag agacgcggtt tccgggcgca
     8641 tttgcgcttc gaaggcataa gggtcggact tcagacgcaa gttgccattg gcatcaatca
     8701 tctcgtattt atagagctga ccgttatgcg ccccagggat aaacagttcc cagatgccgc
     8761 tctctttacg caggcgcatc gggtgacggc gaccgtccca gtagttgaat tgcccaacca
     8821 ccgagacccg acgggcgttt ggagcccaga cagagaaacg cgtacctgtg acgccatcca
     8881 tagtatctgc atgcgcgcct aaggtttcat acgggcgcag atgagtacct tcagataata
     8941 gccaggcatc catttcctgg attagcggac caaaacggta aggatcatca atcaggtttt
     9001 gctgaccatg ccagacaaca gccaactgat agcggaaaaa attcttacgt cgcggaatga
     9061 cgccgctaaa gaatccccgt gagtcgagac actccagttt tgcgagtttg cgcccggttt
     9121 tcggttcaat cacccacaca tcggtagcgt cgggtaaaag ggcacggact tccagtcccg
     9181 cggtggtttt atgcattccc agtacggaaa aaggatccgc aaaatggcct gcaattagcg
     9241 cgttaatcac gtctctatcg atacgatcgg acatgcttgt cttcctgttt tattgtgtca
     9301 ccccatccaa ttggattttt gacttctggt tgtgacattt ttttgacctg aacggcgcag
     9361 cactctgtgc atcctctctg cgtcgtcctc acttcaggta aggctgtgaa tactcatgta
     9421 ttcagccacc cttaaagaat agccaatgct ctatttaact cccggtaaat catgaaacat
     9481 ctgcgcttac tcctgtatta cgcactaaca ggggcggcat cgcgccccag atttaatgaa
     9541 taaagattac gccagttgac gaagcatccg acgcagcggc tccgcggccc cccacagcag
     9601 ctggtcgccc acggtaaagg ctgacaggaa ctctggtccc atattcagct tacgcaggcg
     9661 gcctaccggc gtggtcagcg tgccggtaac ggcagctggg gttagctcac gcatagtgat
     9721 ttcccgatcg ttcggaacga ctttcgccca cggattgtgc gcagccagca gttcttcgac
     9781 ggtcggaata gacacatctt ttttcaattt aatagtgaat gcctggctgt ggcagcgcaa
     9841 tgccccgaca cgcacacata aaccatctac cggaattacg gaagatgtgt tgaggatctt
     9901 gttggtttcc gcctgccctt tccactcttc gcggctctga ccgttatcga gctgtttgtc
     9961 gatccacgga atcaggctac ccgccagcgg cacgccaaag ttatccaccg gcagctcacc
    10021 gctacgggtt aaggttgtga ctttgcgttc gatatcgaga atagcagagg acggggtcgc
    10081 gagttcatct gccacatggc catacagatg gcccatctgg gttaataact cacgcatatg
    10141 tcgcgcacca ccgccggaag cggcctggta ggttgcaacg gacacccaat caacaagatc
    10201 attggcgaat aaaccaccca acgacatcaa catcaggctt acggtacagt taccgccaac
    10261 aaaagtcctg atgccattat ttaatccgtc ggtaatgacg tcctgattga cggggtcaag
    10321 aatgatgatg gcgtcatctt tcatgcgcag agacgatgct gcgtcaatcc agtaaccttg
    10381 ccatccgctt tcacgaagct ttggatagat ttcgttggta taatcgccgc cctgacaggt
    10441 cacaatgata tcgagggcct ttagcgcctc cagatcaaag gcatcctgaa gtgtgccagt
    10501 ggttccgcca aaagacggcg cagcctggcc aagctgagaa gtagaaaaga agacagggcg
    10561 aatggcgtcg aagtcgcgct cttcaaccat gcgttgcatg agaacggagc cgaccatacc
    10621 gcgccagccg ataaaaccaa catttttcat aagcgttttt tcctgcaaag atgtgtgctg
    10681 tataaatgtg ccggtctcct cttggcacat ctttcaccat acaaaaagca gccaaagtcg
    10741 caagtgaaat taatcaatga tagcgaagcc atcagtaatg cgacttatcc tgctttgtta
    10801 gcacgcagaa agtccgcggc aattatcagg gaatttgagt tatgaatgaa atcatttctg
    10861 cagcagtttt attgatcctg attatggatc cgctcggaaa cctgcctatt ttcatgtccg
    10921 tactgaaaca tactgaaccg aaaagacggc gggcaatcat ggtgcgagag ttgcttattg
    10981 ctctcctggt gatgctggtg ttcctgtttg cgggtgagaa aattctggca tttctcagcc
    11041 tgcgagcaga aaccgtctcc atttctggcg gcatcattct gtttctaatc gccattaaga
    11101 tgattttccc cagcgcttca ggaaatagca gcgggcttcc ggcaggtgaa gagccattta
    11161 tcgtgccgtt ggcaattccg ttagtcgccg ggccgactat tctcgccacg ctgatgttgt
    11221 tgtctcatca gtacccgaat cagatgggac atctggtgat tgctctgctg ctggcctggg
    11281 gcggcacctt tgtcatcctg ctacagtctt cgctattttt acgtctgctg ggcgagaaag
    11341 gggtgaacgc acttgaacgc ctgatgggat tgattctggt gatgatggca actcagatgt
    11401 tcctcgacgg cattcgaatg tggatgaagg ggtaacgagg agagtttttc gaaaagagcc
    11461 cggtagcgtc acaactaccg ggcgaacaaa cttaactcaa cagctggaac gcaatcatcc
    11521 caacgatggc accaacagtg ccgaggatgg tttccatcat ggtccaggtt ttcagcgttt
    11581 cggcttcggt cgcgccggta aatttaccga acaaccagaa accagcgtcg ttaacgtggc
    11641 tgacaacaat cgaaccacca gcgatacaaa tcgacagtgc cgccatttgc gcaccagagt
    11701 agttcagttg ttcaatgact ggcatcacca gtcccaccgc cgttaaacag gctacggtgg
    11761 cagccccctg aatgatgcgc actgcagcag ccagcacgaa gcaggtgata gcaatcggca
    11821 ggcccatgcc ggttaacgct tcgcccagtg ccggacctac gccagagtca accagcacct
    11881 gtttgaacac gccgcctgca ccaatcacca gcagaatgat ccccgccggt tgcagcgcgt
    11941 gaccgcaaat ctccatcact ttgtcttttg gcatgccctg acgcattgcc aggccataaa
    12001 tcgctaccag acaagcaacc agaatcgcgg taaacggatg accaataaac tcgaaccatt
    12061 cgtaagcggt agagccttct ggcacaaaac gcgcggcaat ggttttcagc cccaccagca
    12121 ccagcggcaa caggatcagc gacaggctga atccgaaaga tggcatttta ccttcgccga
    12181 gatgcggttc gctgatgtcg tcaggaatat gcagctcaac gtaacggctg atgaagttac
    12241 cccacagcgg cccggcaata atcattcccg gaattgccgc acacaggcca atcaggatca
    12301 tccagccaaa gtcggcattc atctgcgatg ccagcagcat tggcgctggc ccaggcacca
    12361 ggaacgctgc cgcggccgcc acgcctgcga ataatgggat taccagcttc accaggttcg
    12421 taccggtgtg gcgcgccatt gagaaagcaa cgctaatcag cagaacaatc gccacttcaa
    12481 agaacagcgg tagcgcacag accagccccg caaggccgat ggcataatgc gcgcggctgt
    12541 gaccgaagga tttgagcatt ttgacggcaa tctgatcgac tgcgccggtt tcatataaga
    12601 tcttgccaaa catagctccc agggcgacaa ccaccgccag gaagccgagg gtgcctccca
    12661 tccctttttc catcgtcgct gcgattttat cgagcggcat accggaaaaa aggccagccc
    12721 ccatggacac caccatcaaa gccaggaaag cgtgcatacg cgccttcatg actaaaaaca
    12781 gcagcagtaa aacagaccct actgctgtta aaacaagcgt taatgtagtc actacttatt
    12841 tgcctttttt aataacctca atggtgcttg ccacaacacc ttccagcggt tgatcgatat
    12901 ccaccaccag tacatcggtt tcgtccgcac ccggctcctg cagcgtttca aactgcgtca
    12961 ccaacatttg ggttttaaag aaatggcctt tgcgcgcttt caggcgactt tcaatcacat
    13021 caaaatcgcc tttcaggtag atgaaagaga gattcggatt accttcacgc agcaagtcgc
    13081 gatagtgctt tttcagtgca gaacaggcga tcagcgacac tttattggtg cgctgcatag
    13141 caaacgcgga gtcgttcagc gcctgcaacc agggtttgcg atcgtcgtca ttcagcggtt
    13201 cgccagacgc cattttttcg atattgcgcc gtggatggag gaaatcgcca tcaagaaacg
    13261 cggcatgaag ttgatgcgcc acttcactgg cgaccgcaga tttgccgctg cccgatacgc
    13321 ccatcaagac gtaaatgtgg tgatcatggt tagtcgtgct caaagtggtg cccccacaat
    13381 acaagaatta aaaatgttac gggtaactgt tatcggcaac attgtccagc cggacaatat
    13441 cagaagcaat atccatccgt gccttaagtg tataagtgtg agctacttca aatttgtggg
    13501 cttaaataga tccgcccggt gacaaggtga aacctaaatc taacattttc ggtgtcacag
    13561 attcgccacg aatacgcgcc agcaggcgtt cagcgccaat actgcccatc cgctcacgcg
    13621 gcgtcagcac gctcgcaagt cgtggctcca tcacctgacc aatgtcatga ccgtggaaac
    13681 cggcaatcgc catatcgtca ggaactttta accccagacg ctgacattca aacgccgcgc
    13741 cgaccgccag atcatcatta gtacagaaca cgccatccag ctgcggatat tcccgccgcg
    13801 cctggcgaat cagttcaata ccggaagagt aagaagaaga ttgctcaacc atcacgctat
    13861 atggcaccag gcctgcatcc agcatcgcct gttcgtatcc cttctgtttg atgatagtac
    13921 gttcgtcgag acgtgcgccg agataggcaa tgtggcgatg cccacgagca ataatggcgg
    13981 tagtcatctg gcgtgccgct tcaaagttat caaaaccgac ggcgatatcg aggcacggcg
    14041 actggctgtc catcaactcc accaccggga tccccgccac ttcaatcatc tttaaggtgc
    14101 gcggcgtgtg ggtacgttcg gtgaggatca ggccgtcgat attccaggag agcatggatt
    14161 cgagacgttc ttgctccatt tccggtttat aaccgtagtg cgccagcatg gtctgataac
    14221 cgtgcgcgtc ggtgacgctt tcgattccgc gtaatacttc cgcgaaaacc tggttggtga
    14281 gagaaggtaa caggacgcca atcgcccggc tggtagcgtt agagaggata tcgggcgcac
    14341 gattgggaat atagcccagt tcatcaagag cggcggcaat cttgccgcgt agagcgacgg
    14401 aaacctgctc cgggttgcgt aaaaaacggc tgaccgtcat tttggtcacg cctacacggt
    14461 cagccacatc ctgaagtacg ggtcttttct ttttcatcgt cctgaaggta caaaagagat
    14521 agattctctt agtttaacac ggacgcatca gaaccttccc ggggaaaaca gtgcttgttt
    14581 acaattatta gatatacact aaatcaattg catcgcattg tgctaacgac gcggcaaaac
    14641 cccggaagcg tgcatcagca cgcgaccgga gcgagcgagg aagcctgcaa aaaggcaggc
    14701 ttcaaagatg ccgttgagtt ttaaaccggc ggcagatcga acagtaacac ttcgctgtcg
    14761 ctatccgcat ggatggagat tgcctgctca tcccagattg ccagaccatc gctggtcgag
    14821 gctttcacgc cgttaatggt aacattgcct tttaccacct gaatccagac gcggcgttca
    14881 gcggcaatct gatgcaccga ctgctcatct ttcagcaacg cccaacgata cagttccata
    14941 tcctgatgca ctttcagcga accatctcgc gcatccggcg agagcaccag ctgtttgccc
    15001 tgcacggcat cgaagcgacg ctgttcataa cgcggcgtaa tgccattttc ttcaggcatg
    15061 atccagatct gatacagatg cagacgctcg gtgctgcttg ggttgtactc tgagtgacga
    15121 atacccgtac ccgcactcat aatctggaac tcacccgccg gaacctgctc tttattgccc
    15181 atgctgtcct gatgctcaac agtaccttcc agcacgtagg tcaaaatttc catatcttta
    15241 tgcgggtgag tgccgaagcc ctgccctgct tcaatcacgt cgtcgttaat cacgcgcagc
    15301 gcggagaagc ccataaagtt cggatcgtaa tagttggcac aagagaaagt atgccaggag
    15361 tccagccagc catgatttgc atgaccacgt tcatttgctt tgcgtaagta gatcatttgt
    15421 attcgccccc tgaatgattt cgatgggctt agtgtggacc cgatctgcct gggatgatag
    15481 agggtgaaaa ttgacccctc tgttcaaaaa aattgaacta ttcgaggggc catcaaactt
    15541 aattcgcgag agtcactgtg gagggagagg cttgctcaaa tccgcgttca aggatttcca
    15601 gattggtaag aacttcagat tccttgacgt aatttggcgc accgtgggtg atggtttgat
    15661 acaacgcatc ataaacacgc ccgtaatcgc ccatctccgg cttcatctct tctctgaccg
    15721 tcacaccctc gtcattgaca tactccagca caccgaccga atcatccgct gcgaatcccg
    15781 gttcgcccgg cataatatta gccttcaggc tggtttcctg ctggtcgata ccgtatttaa
    15841 taaacgaacc tttcttaccg tgaacgataa atttcggata atcgattttc accagatggc
    15901 tggttttgac gatggctttc aggtcgccat aaaacagttg cgcttcaaag gtgtcgtcag
    15961 gattggcttt attacgcagg ctgcggatgt cataagcgac gtgatccggg cgaccgaaca
    16021 gagaaataat ctggtccatc gtatgcacac caaggccata gaacgcgcca tcctgcggca
    16081 gtccaggttt ggtttctgcc accgggcggt aatagtcaaa atggctttcc acttcgacaa
    16141 tctctcccag cttgccgctt tcaatcgctt ttttcgccgt caggaagcag gaatcaaagc
    16201 gacgattctg atatggcgtg acggtcagcc ctttgctttt cgccagtgca aacagctctt
    16261 tcgcctgcgc aagtgtcgga gtgaacgttt tttcgaccag cacatttttc ccggcttcca
    16321 gcgcgcgctt cgcgtactcg aagtggctgt ccgcatgggt gcagacaaca accaacttaa
    16381 catcgggatc gtttagcact tcgtcgagat cgctggtgaa atggatatga gagtaaatgg
    16441 gagcctgttc ttccggcttc gcatggcgac gaaaaatatg cgcgacatgc cagctatcct
    16501 tgcggttaag tacatacggc agatggtaac gggtggtgct tttgccgaag ccaataaagg
    16561 cgcagttgat gaccatgatt tcgtcctttt taaggtggtt atttacacct tagcgcaaag
    16621 cggacgtggt tcctactgga gtgcgcataa cgaacacaag cactcccgtg gataaattga
    16681 gaacgaaaga tcaaaaaaaa agccagcacc cggctggcta agtaatactg gaagcaatgt
    16741 gagcaatgtc gtgctttcag gttctccgcg agggtcttcc tgatcgcgat acaataataa
    16801 tcattctcat tcgcacttgt ccaacacttt ttgcaaaaaa atgcatttga ctcgcatttg
    16861 aaagtcaatg atgttgaaag ggacatttac cccaaagagg acaaaggaat gagtgagata
    16921 gtaatacgcc acgcagaaac acgggattac gaggccatca ggcagattca cgcccagccg
    16981 gaggtgtatt gcaacacact acaggtgcct catccttccg atcatatgtg gcaggagcga
    17041 ctcgccgatc gtcccggcat caagcaactc gtcgcctgta ttgatggaga cgtcgtgggc
    17101 catctcacca ttgacgtgca acagcgccca cgccgcagtc atgttgccga ttttggtatc
    17161 tgtgtcgact ctcgttggaa gaaccgcggc gtcgccagcg ccctgatgcg agagatgatt
    17221 gaaatgtgcg acaactggtt acgggtagat cgcattgaac taaccgtgtt tgtcgataac
    17281 gcgccagcaa ttaaggtcta taaaaaattc ggctttgaga ttgaagggac cggtaagaag
    17341 tacgcattgc gtaatggtga atatgtcgat gcgtattata tggcgcgggt gaaatagaga
    17401 atttgcaatc taagtttgtc atttaagccc tcacctaccg ggagggctta agatattaat
    17461 accccgccgt taaatcatcc accgagcgcg ggtcggatgc gccgtacaac tcaccgtccg
    17521 gcccaaccat aatgctttgt gtgctgccca tcgcctcttt cagcgccact ttctgacctt
    17581 ttgcttccag cagcttgagc gtatccgggc taaacccttt ttcgacacgc agctcgtccg
    17641 gcaaccactg atggtggaaa cgcggcgcat tggtcgcttc ggcgacgttc atgccataat
    17701 cgatgctatt caccaccatt tgcagcaccg tggtgataat ccggctaccg cctgggctac
    17761 cggtaaccag ccaggtttta ccgtctttca ccacaatggt cggcgacatc gacgacagcg
    17821 ggcgtttgtt cggcccgacg gcgttggcat caccgcccac cagcccgtaa acgttcggta
    17881 cgcccggttt ggcggagaaa tcatccatct ggttattaag cagaataccg ctctcacccg
    17941 cgacaatacc cgtaccgaag gtggtgttca gcgtataggt caccgccacc gcgttaccgt
    18001 ctttatccac tacagagtaa tgggtagttt gattactctc ataaggcgca agcttgccag
    18061 ggcgaatctc actggacggc ttcgcttttt tgatatcaat ttgatcggca atggatttgg
    18121 cataggcttt attggtcagt gcctgccacg ggactttgac aaaatccggg tcgccaagat
    18181 attccgagcg gtcggcgtag gcgtatttct ccgcttctgc catgatttgc atcgcatcgg
    18241 cgctgccaaa gccgtatttc ttcatatcga agttttccag aatattgagg atttgtacga
    18301 tatggatccc gccggaggat ggcggtggca tggagtaaac ctgatacccg cgataatcgc
    18361 cgcttatcgg agtgcgttcg accgctttat aggccgctaa atcttcttta gtgatcaagc
    18421 caccgttttt ctgcatctcc tgggcgatct gttccgcaat cgtgccttta tagaattcgt
    18481 ccgggccgtt ttcagcaatc atctccaggc tctttgccag gttcgcctgc accagcgtgt
    18541 cgcccttttt cagcggctcg ccctctttcc agaagatagc tttactgttt tcgtgattcg
    18601 gcaacacttc gctaccgtag gttttgagat cgtcagccag cgcgtcgtta acgataaaac
    18661 catcgcgtgc cagtttaaac gcgggctgca cgactttgtt cagcggcatg gtgccgtatt
    18721 tatccagcgc cagcgagaaa cctgctaccg tacccggtgt gccggaagcc agatgcgaag
    18781 tgagtgattt tttgctgtcc gggttgccct gatcatcgag gaacatatcg cgggtcgctt
    18841 tggcgggtgc catttcgcgg aaatcgatag ccgtggtatt gccatttttc gagcggatta
    18901 acataaaacc accaccgccc agattccctg cctgcggatg cgttaccgcc agcgcgtagc
    18961 ccaccgccac ggcggcatca acggcattcc cgccctcctt gaggatatcc acccccacct
    19021 gagtggcagt ggcgtccaca gacgctacca ttccctgttt cgcgcgtacc ggatggaaga
    19081 catcttcctc cacaccatac gagacgggcg gcgcaggagg cgcggcggcg gcgctaaaac
    19141 aacttcctga gagcagagca gcaatggcca cccggcgtaa aaacgtcggt tttatcatcg
    19201 ttattctcca gagattaagg ggcaacccca gctaagcctg gtatataact ctgaattaat
    19261 catcgttttg tcgggaagcg agtaaactta aaggatatct tcagaggagg atacgcgatg
    19321 aaacgacttc tgattcttac ggcactcctg ccgtttgtcg gctttgcaca gcccattaat
    19381 actctgaaca accctaacca gccggggtat cagataccca gccagcagcg gatgcaaacc
    19441 cagatgcaga ctcagcaaat ccagcaaaaa gggatgctga atcagcaact gaaaacgcaa
    19501 actcagttgc aacagcagca tttagaaaac cagataaaca ataattctca gcgggtgttg
    19561 cagtcacagc cgggggagcg aaatcccgcc cggcagcaaa tgctgcccaa caccaacggc
    19621 gggatgttaa acagcaaccg taatccggat agttcgttga atcagcagca tatgctgccg
    19681 gagaggagaa acggcgacat gctgaatcag cccagcacgc cgcagcctga cattccgttg
    19741 aaaactattg ggccgtaaag ttcggaccaa tcacgtcaat cgcatcggta cagatgcaat
    19801 ccacgcccca gcgcagcaac tctgccgcgt gctggggttt attgacggta taaaccagaa
    19861 tccgtaatcc ggcgtctttc aactgcatca ctcgcgcttt atcgagtaac ttatgattga
    19921 gatgaataga gacgcagccc agccgcgcgg tcagttcgcg ccagtcgtcg cgccactcat
    19981 caagcaataa accacgcggc agttccggtc ccgcctgttg tgcagcttct aaagcatcaa
    20041 tctcaaacga tgacagcagc ggcggcgtca taccggccca cagttggcgt gccgccagcg
    20101 ccaccatttt gcccgttaat ggtccagtgc cagtggtggg tttgatttcg atattcgcca
    20161 tcatcccgtg ttcgcgacag cgttccgcca cctgcgaaag caacggcagc ggctcacctt
    20221 taaacgcttt gctgtaccaa ctgcccgcat ccacgcgcag taaatcctgc cagttcagtt
    20281 cacccgcgac gccccagccg ttgctggtgc gttcgagatt gtcgtcatgg agcaggaaga
    20341 tctcgccatc tttcgataac ttcgcgtcaa attcgatcat cttatgaccg tattttgccc
    20401 cgacgtcgat tgccgccagg gtgttttccg gggccagctt accgccgcca cgatgagcga
    20461 cgatgcgggg ataaggccag ttactcatac tcgttgtcct gtttcaccat caaaaagatg
    20521 cagctgattt tccgccagat gcagccacag cgtgctgcct gccgtcgggc gctcctgatg
    20581 cgccagtcgc accaccagct tctgcttgcg agcttagcgc aatatgttcc gggcgaatgc
    20641 cgagggtcat tttacgcccg gcatactgac ggtagccacc gtttagcggt agcacgatac
    20701 cgccgtccag ttcgaaatgc gtgccttcat tattcatgcg gcctgtcagc aggttcatcg
    20761 ccgggctgcc gataaaactc gccacaaaca ggctggcggg cttttcgtag acttccaccg
    20821 gcgtgccaat ctgttcggca acgccgccgt tcatcaccat tactcgctgg gcgagcgtca
    20881 tcgcctcaac ctgatcgtga gtaacgtaga gtgaagtcgt tttcaggcga cggtgcagct
    20941 gttgcagttc aagacgcatc tgtacgcgca gcttggcatc gaggttagag agtggctcat
    21001 caaacaagaa caccgccgga tcgcgcacaa tcgcgcgtcc catcgccaca cgctggcgct
    21061 gaccgccgga aagctcgcgc gggcgacgtt tgagcagacc gtccagctcc agaatgcgcg
    21121 ccgcttcttt aacgcgctcg gctatttgct gcttgcccat gccgcgaatt ttcagccccc
    21181 acgccatgtt ttcttcgacg ctcatatgcg gataaagcgc gtaattctgg aacaccatcg
    21241 caatcccgcg atctttcggc tccatttcgg tcacgcgctg gtcgttgatc cagatatcgc
    21301 cttcggtcac cctctccagc ccggcaacca tacgcagcag cgtcgatttc ccgcagccag
    21361 acggcccgac catcacgata aattcgccat ccgccacatc aagggtcagc ggtttaatga
    21421 cctaggtttt gccatcccag cttttggtta ctgcctgtaa tttcagtcct gccatcttat
    21481 ttctcactat cgaccaggcc gcgcacgaag gcacgctgca tcactaaaac aatcactact
    21541 ggtgggatca gggttaacaa catcgccgcc atcactgagt tccattccgt ggtgccttcg
    21601 ccagtggcga tcatcccttt gatccctgcc acggtggtgc cgagatccac gtcggtaata
    21661 atcaacaacg gccacaaata ctggttccag ccgtaaataa aggtgatcac aaacagcgcc
    21721 gccagattag ttttggagag cggaaaaacg atgtcgcaaa agaagcgcat tggtgacgca
    21781 ccgtcgatcc gcgccgcttc caccagttca tccggcagcg tcataaagaa ctggcgaaac
    21841 agaaacgtgg cggtcgccga ggccatcagc ggcagcgtta aaccggcgta gctgtcgagc
    21901 atcttcaggt tggcgatgac ttccaccgtc gggaagatac gtacttcaac cggcagcatc
    21961 agggtgataa aaatcatcca gaagaagagg ttacgtagcg gaaaacgaaa ccagacaatg
    22021 gcaaatgccg agagcatcga gacagtaatt ttgccgagcg taatgctgaa cgccatcaca
    22081 aagctgttaa gcaacatccg ccagaacggc gcgctatttg tgcctacccc gttcacccag
    22141 atgttgtgga tgttttccag cagatgcgtg ccggggatga gcgtcatcgg cgcggcgtag
    22201 acggcctgtt tatccagcgt cgccgcgaca aacgccacgt acagcgggaa gaggatcacc
    22261 gcgatcccga ggatcagcat ggtatggctg aatatcgtca gccacggacg gttctcaatc
    22321 attggtaacg caccttgctt tcaacatagc ggaactgcac caccgtcagc acgatgacga
    22381 ggaacatcaa caccatcgac tgtgcggcag acgaagccag atccagtccg gtaaaacctt
    22441 cgcggtagat cttataaatc agcgtcgtgg tggcctgaac cggcccgccg gacgtggcgg
    22501 cgtcgatcac cgggaaggtg tcgaagaagg catacaccag gttcactacc agcaggaaga
    22561 aactcaccgg ggcgataagc ggcagcgcaa tcttaaagaa gcggcgaatc ggccctgcac
    22621 cgtcgatggc tgcggcttcg atcaacgaac ggggaatgga ttgcagcgcg gcatagaaga
    22681 acagaaagtt gtagctgatt tgcttccaca ccgaggcaaa caccaccaga aacatcgcct
    22741 gaccgctatt ttgcgcgtgg ttccagtcgt agccgaactc agcgagaaaa tgggtgatca
    22801 gcccgcgacc ggggttaaac aggaagatcc acaatacggc ggcaacggcg ggagccacgg
    22861 catacggcag caacattaag gtttggtaga aacgactgcc gcgcacgatg tactccacca
    22921 gcgccgcaaa gaacagcgat accagcaaac cgctgacggt gacaaaggtg ctgaatttta
    22981 tcgtcgtcca gaaggagtcg agataatagc tgtcatgaaa cagcgtgacg aagttatcca
    23041 gaccgacaaa ctggctggag aaaccaaacg gatcgacgct ttgtagcgag taccacaacg
    23101 cttcgcccgc aggccagata aaaaagataa cggtgatgat gagctgcggc gcgaccagca
    23161 gataaggcag ccagcgcgag cggaacaccg gacgggatga tgacattacg gttaattcct
    23221 gaactgtgcc ggatgcgctt cgcttatccg gcctacacgc gatgccaggt cagataagcg
    23281 aaacgcatcc ggcattacac tgattaagac ttcgtcgatt tctcaaagcg gcgcagtaac
    23341 tggttcccac gctcaactgc ggtatccagc gcctgctgtg gcgtcttctt accggtccac
    23401 acgctctcaa gctcttcatc cacaatcacg cggatctgcg gcatgttgcc cagacgaagc
    23461 cctttggtga acggcaacgg cggcttgttc agcatctgac gcgtcgcggt atccgcacct
    23521 gggtttttct cgtaaaagcc ctgctcacgg gtcaggtcat acgccgcttt ggtaattggc
    23581 agataaccgg ttttctgatg ccactcagca gcgttttctg gcttcgcgag gaagtcgaga
    23641 aacttcgcca cgccggtata cgtttcttta tctttgccct gcatcaccca caggctggct
    23701 ccgccgataa tggcgttttg tggcgcatct ttcgcatcgg cgtcgtaagg catcatacct
    23761 acgccgtagt taaatttggc gtactcgcga atgttggcaa gagaaccgga agaggcggtg
    23821 gtcatcgcgc aatcaccgtt atagaacttc tcggtggatt catccttacg accgacgtag
    23881 ctgaagtcgc ccttcttgtt catctcctcg agcatggcga tgtgtttcac ctgctccggc
    23941 ttgttgaact ccagcaccgc gtccgtaccg tcaaagccgt tgtttttgct ggcaaacggc
    24001 agaccgttcc aggcgctaaa gttttccagt tggatccagc cctgccagcc gctggcgtag
    24061 ccgcacttaa taccggaggc tttcagtttt gcggaatagt ccgccagatc ctgccaggtt
    24121 ttcggcggct gttccgggtc taaccccgct ttcttgaagg cgtctttgtt gtaatagaga
    24181 acgggagtcg agctgttgaa cggctgggag agtaagtggc cggttttgct gtcggagtag
    24241 taaccggaaa ccgtcggcac aaactgcgac tcatcgaact gaatccctgc ctctttaaac
    24301 acgtcataca ccggtttaat ggctttcgac gccatcatgg tggcggtgcc aacttcataa
    24361 acctgcaaaa tagccggagc gttgccggta cgaaatacgg caatccccgc gcttaaattc
    24421 tgttcgtagt tgcctttata ggtcggtaca attttgtaat ccggattttc ggcgttaaaa
    24481 cgttgggcca gagaatccac ctctttaccc agttcccctt ccatagaatg ccagaacgga
    24541 atggtcgtca ctgcctgtgc attccccatt aacgccagtc cgagcgccag tgctgaagct
    24601 gtataatgta acggtttcat cgtttatctc tcttgttgta ccgaatgcgc gaattcacgc
    24661 gttttatgct cgcggggtaa catgacatgc tcgaattaca gaaaaataac ttttttgtta
    24721 catttgtaag atagtaaggt gtcagaaaga tgacaaggcg gtgacggcgt gggtgaggga
    24781 aaatgggaga tggggcacgg ataagcggaa aaatagaagg tctgaatcaa actctacaga
    24841 ttactcatcg tttcatgccg gatgcggcgt aaacgcctta tccggcctac aagatcgtgc
    24901 aaattcaata tattgcaact cacccagtag gcctgataag cgcagcgcat caggcaattt
    24961 tgcatttgtc acctgtctca aaggagtctt ttgactcctt atcaatcaac gtgttattac
    25021 ccgcctaaat acgcacttct cactgcttcg ttcgccagca gcgcgtcgcc ggtatcggag
    25081 agcaccacat ggccgttttc cagcacgtag ccgcgatccg ccagctttag cgcctggttg
    25141 gcgttctgct cgacgaggaa gatagtcatc ccctgctcgc gcagctgctc gatggtgtcg
    25201 aaaatttgct ggatgataat cggcgcaaga ccgagcgatg gctcatcaag taacaacaaa
    25261 cgcggattgc tcatcagcgc gcgaccaatc gccagcatct gctgttcacc gccggacatg
    25321 gtgcccgccc gctgaacacg gcgctcatgc agacgtggaa acagttcata cacccacttt
    25381 atgcgctcct gaaactggtc acgttcggca aagaagccgc ccatcgccag gttctcttcc
    25441 accgtcatcc gcgagaagac gcgacgccct tccgggacaa tcgctaccgc ttcgcgcatg
    25501 attttcgccg tctgccagtc ggtaatgtct ttatcatcaa acacaattcg cccgctggtg
    25561 gcgcgcggat cgccgcataa cgtgccgagc aaggtggttt tccccgcccc gttcgcgcca
    25621 atgagagtga caatctcgcc ctgattgata tgcaggctca cctcatgcag cgcctggatt
    25681 ttgccgtagt gggcgctgac tttgtcaaag gacaacatga ctttttccat cttatgcctc
    25741 acctaaatag gcacggatca cgtccgggtt attacggatt tgctccggcg taccgtttgc
    25801 cagtggcgtc ccctgattga ccacataaat tcggtctgaa atccccatca ccagcttcat
    25861 atcgtgttca atcaacaaga tagtggtgtt gtgatgattg cgcagttcgg caatcagctc
    25921 atccagctct ttggtttctt ttgggttaag acctgccgcc ggttcgtcga gcattaaaat
    25981 ctccggctgc gtcaccatgc agcgggcaat ctccagacgg cgttggtcac cataggccag
    26041 gttactcgcc tgacggttgg cgtgttccag caaaccaatg cgctcaagcc aggtggcggc
    26101 gcggtcgagc gcttcgctct gggcgcgacg gaaagaaggc gttttcaaca ggccagagaa
    26161 cagcccggtt ttcagttgct gatgctgcgc caccagcagg ttttcaatta ccgtcatttc
    26221 acggaacaga cgcacatgct ggaaggtgcg caccacgccc atgcgagcaa tttgctgccc
    26281 cggtaaacct tcaagatgct gatcgcgcag taaaatggtg ccgccggtgg gtttgtagaa
    26341 accggtcagg cagttaaaaa ccgtggtttt tccggcaccg ttagggccga ttaacgagac
    26401 gatttcctgc gggtacagtt caagattgac gttgttcacc gccagcaggc cgccgaagcg
    26461 catcatcaga ccgttaacag ataataatgg ctgactcatg cctgctctcc tttcgctgcg
    26521 ccgtttttca gcttcagttg cgggcgcgtc atgggcagca aaccctgcgg acgccagatc
    26581 atcatcagca ccatcaaacc accgagcatt aacatgctgt attcgttgaa atcacgcatc
    26641 aattcgcgcg acaccaccag cagaacagcc gccagaatca ctgcaaattg cgagcccata
    26701 ccgccgagca ccactatcgc cagcacaaag gccgattcag caaaggtgaa ggattccggg
    26761 ctgacaaagc cctgacgcgc cgcaaacagc gttccggcaa aaccggcaaa tgcggcgctg
    26821 atggtaaaag cagtcagctt gatacggcgc gggcttaagc ccagcgaacg acaggcgatc
    26881 tcgtcttcac gcaacgcttc ccacgcgcgg cccagcggca tccgcagcag gcggttaatg
    26941 acaaacaggc ttaacaccac cagcagcaac gccaccaggt agaggaagat aacgcgatcg
    27001 gacggatcgt atttcaggcc aaagaaatta ctgaacgtgt cccagccgcc ttcacgagcg
    27061 gtacggctga actcgagtcc gaagagtgtc ggtttcggga tctgactgat tccgttcggg
    27121 ccgccggtga tttcagtgtt attgagcagc aatatgcgca caatttcgcc gaaaccaagg
    27181 gtaacgatcg ccagatagtc accgcgcaga cgcagcaccg gaaaaccgag caggaaaccc
    27241 gccgccgctg ccatcagtcc ggcaatcggc aaacaggtcc agaaccccag accgtaatag
    27301 tgattgagca gcgcaaaagt gtaagcgccg atggcgtaaa aaccgccgta ccccagcacc
    27361 agcagaccag aaagaccaac aaccacattc agcccgagac cgaggataat gtagatcatg
    27421 gtcagagtgg cgatatccac cgtcccacgt gacaccataa acggccacgc caccacaagc
    27481 accagcagcg ccacgaggaa cagcttttgc ttcaccgtgg acccatcaat cgcgggcaga
    27541 ataaacttcg gcccggaaac gcttttcaac cctttctgga aagccggtcg caaaagctgg
    27601 aagaaaaaga ccaccgccgt gccgataaac atccactgcc aacggatgtc tgaagccgtg
    27661 tcgaccacca gtttggtgcc atccagctcc agttgtacgc ccataaagac gcccgccagg
    27721 acaaagaaca tcgcggcaga gagcagtgcc attgcaatat gcatcggttt catactttct
    27781 ctacctccgg gcgacccaga ataccggtcg gcatcaccag cagcaccaga atcagcaggg
    27841 cgaatgagac cacatcttta tattccgtac tcagataggc agaagagagc gcctccgcaa
    27901 tccccaggat cagcccgcca atcatcgccc ctggaatgct gccaatccca ccgagcaccg
    27961 cagcggtaaa ggctttcatc ccggccataa agccgatata ggggttaatg acgcagtaga
    28021 actgaccgag cagtacgcca gccactgccg ccatcgccgc gccaataaca aaggtcaaag
    28081 caatcacccg gtcggtgttg atgccaagca ggctcgccat tttcagatct tccgcgcagg
    28141 cacgacaggc gcgccccata cgggaatagc gaatgaaaat cgtcagcgcc agcatggcga
    28201 ggaaggtgac aatccagatc accgcctgca tggtggtaat agaggcagag aagttttcgc
    28261 tatggcccac cacccactga ccgttaaaca ggctcggcag cgccacgtcg cgcgaacctt
    28321 cggtcaggct gacgtagttt tgcaggaaga tggacatacc gattgcggaa ataagcgcaa
    28381 tcaggcgctt agagttacgc accgggcggt aagctacccg ttcgatactc cagccgtagg
    28441 cgctggcgat gacgattgcg ccgacgaatc ccgccgccac cagcagccag ccggtatcaa
    28501 tgcccatcat catcagcgcg gcgatgatca taaatgagac gtagctgcca atcatataaa
    28561 cctcgccgtg ggcgaagttg atcatgccga taatgccgta aaccatggtg tagccgatgg
    28621 ctatcagcgc gtaggtactg cccagcgtga cgccgttaaa catctgctgc aagaaataca
    28681 aaaactgctc agacataagg taacctttct aaacccgccc gcattttacg ggcggtggga
    28741 tgatcacttg gctgccgtgg atgaaccgtc ggcgtgccac tggaagacac caaaatcaaa
    28801 tcccttaaga tcgccttttt catcccagtt cagcggccca atcacggtgt ttgcaccgtt
    28861 agcttttaaa tctttcacca gcgccagcgg ctcatcgctg ccggtacgct caagggcagt
    28921 tgccagagat tgcactgccg cgtaagtgat ccagacataa ggcccggacg gatctttctt
    28981 gtctgctttc agcgcatcaa cgatgccctg gtttgccgga tcctggtcat agcgttttgg
    29041 catagtgacc aacatgcctt cggcggcatc accggcaatg ttcgacagcg atgcgttacc
    29101 gacaccttcc ggccccataa actgggtttt caggccaacg gaacgggctt ggcgcagcat
    29161 ctgccccatt tccgggtagt aaccgccgta gtaaacgaag tcgatatttt cttttttcag
    29221 gcgggcgatc agcgcggaga aatctttctc cccggcggta ataccgtcga agaagacgac
    29281 gttggcgtta gccgctttca gcccgtcctg caccgaacgc gccagccctt cgccatactg
    29341 ttgtttgtcg tgaatgatgg cgatgcgctg gggcttcacc gtctcaagaa tgtattttgc
    29401 cgccgtaggc ccctgggaag agtccagccc ggcagtacgc ataatgtgtt gataaccgcg
    29461 ttgggtcagc tccgggttgg tcgctcccgg cgagatcatc agaatacctt cgtcttcgta
    29521 gatatccgac gctggctggg tagaggaaga acacagatga ccaataacgt atttaatgcc
    29581 gtcgttaacg attttgttgg cgaccgcaac ggcttgtttc gggtcgcagg cgtcgtcata
    29641 ttccacgcca accagcttgt cgcccttaat tccccctttg acattaatgt ctttaatcgc
    29701 ctgacgcgcg ccgttaaatt ccatatcgcc ccactgggca atcgggccgg acatcgcgcc
    29761 gacaacggcg actttaatat cgtcagccat agcggtgtgt gaaattgcca gtgcaatcat
    29821 ccctgcgatg atagttttcg cattccgttt catagtcaaa aatccccatt cgtgatgtta
    29881 tgttgctttg tttttatgtg ttaacaaatc agactgttct ttttttatac tgcactgttt
    29941 ttgcctgtct gattaagggg ttagcgcagt attttgtgat gatagcgatt aaaatcccta
    30001 tttttcagtc gattaagaac agataatatt ctgaatttat tgatagataa acagaaaaaa
    30061 gtgcctttgt cagcataaaa taacggcaca aagggcggaa taattcacta tcattcaggg
    30121 gattatgctg gacatttttc attctctaat gttttaactt tgtaattatt gctgttaaaa
    30181 aattaatcac ctgccaaaag aaataaaaaa gagaaagcct ccgattaaat tatttcgcta
    30241 cactggttcc acttttgtga tttacacggg ttacccatga agctgaccat cattcgatta
    30301 gaaaacttta gcgaccagga ccggattgac ctgcaaaaga tctggccgga gtattcccct
    30361 tcctctttac aggttgacga taaccaccgt atctacgccg cgcgttttaa cgagcgtctg
    30421 cttgcggccg tgcgggtaac cttaagcggc accgagggag cactggattc tctacgcgtg
    30481 cgggaagtca cccgccgtcg cggtgtgggg caatatctgc tggaagaggt tttgcgtaac
    30541 aatcctggcg tttcatgctg gtggatggcg gatgcaggcg tggaagatcg cggtgtgatg
    30601 acggcgttta tgcaggcact gggatttacg gcacagcagg gcggctggga gaagcgttaa
    30661 tcgtcaagtt gatttcaaaa gtgatgacca acgagaaatt gcctgatgcg ctacgcttat
    30721 caggcctata atgtgtgctg caatttactg atttctttgg ttcttgtagg ccggataagg
    30781 cgtttacgcc gcatccggca tgaagcaacg tattcgatat tagcaatttg gcggcaaccc
    30841 aaagttgccg attaacaatt acttcgcatc ggtcgccgtg ccgttggcgt gccagtcaaa
    30901 tacgccgaac tcaaagccct ttagatcgcc tttctcatcc caggtcagtg gccccatcac
    30961 ggtatccacg gagttcgctt ttaggtattt ggcgatttca gccggatcgt cagactgatt
    31021 caggcccgcc tgcaaagatt gcagcgcggc gtaggtggtc cagacgaatg cgccgcttgg
    31081 gtcctgtttt ttcgctttga tcgcatcaac aatgggtttg ttcgccggaa cctgatcgta
    31141 gttcttcggc ttggtcacca gcagcccttc cgctgattcg cccgcaatgt tagacagcga
    31201 aacgttagcc acaccttccg gccccataaa ctgagttttc agccctgccg cgcgtgcctg
    31261 acgcaggatt tgccccattt ccgggtgata accgccgtag taaacgaagt cgatattctc
    31321 ttttttcaga cgcgccacca gcgttgagaa atctttttcc ccggcggtga taccatcaaa
    31381 gaacaccacg ttagcattgc ctttcttcag gccgtcctgc accgctcgtg ccagaccttc
    31441 gccgtattgc tgtttgtcgt gaacgatagc aatacgctgc ggtttcactt tctcaagaat
    31501 atatttcgcc gccgtcggcc cctggtcgga gtccaggccg gtggtgcgca ggatcagctg
    31561 atagccacgg gcggtcagct ccggcgcggt tgccgctggg gtgatcatta aaatgccttc
    31621 gtcttcgtag atgtcagacg caggctgcgt tgatgaagaa cagaggtgac caatcacata
    31681 tttaatgccg tcgttaacga ctttgttcgc caccgcaacc gcctgtttcg ggtcacaggc
    31741 atcgtcatat tttacgattt gcagtttgtt gcctttaatg ccgcctttag cgttgatatc
    31801 cgcaaccgcc tgctctgcgc cggtaaactc ctggtcaccg tactgcgcaa ccggaccgga
    31861 cattgcaccc acgaccgcga ctttaatatc ttctgccaga gccatattgc tgaatgccag
    31921 cgcgatacat cctgccagta acgctttacc ctttatgttc atcctgagaa tccccattct
    31981 tctggttatt acgtgtgttg tgatgttgtt gttcagcact ttatttcgtt ttatgcatga
    32041 ctacccgtgc tttagcagca tactctgcta aaacataccc gatttttatg atattggaat
    32101 agctattttg acagtttatt aacaatctgc gtggggattg gcgttttgcc ggagggggaa
    32161 ttgattattg tagaggccca aaaaacaaaa accccggact ctcatccagg gttctctgct
    32221 taatagcgga aattacgctt caatagcggc gcgaagtttt ttcatcgcgt tcttttccag
    32281 ttggcgcaca cgttcagcgg aaacaccgta acggtcagcc agttcctgca acgtggactt
    32341 gttgtcttcg tccagccagc gcgcacggat gatgtcctgg ctgcgttcgt ccagaccatg
    32401 catcgcgtcg gtcagacggt ttgccgcctg ctcttcccag ttatcatctt cgatgccgtc
    32461 ggcaaagtta gatgatttat cctgcagata gagcaccggt gccatcggct ggctgtcgga
    32521 atcgtcgtcg gaagacaggt caaaggtcat gtcctgtgcc gccatacgtg attccatctc
    32581 acgtacgtct ttgctggtta cgcccagttc acgggccacc atttcgactt catcctggtt
    32641 aaaccagccc agacgctgct tggttttacg caggttgaag aacagtttgc gctgcgcttt
    32701 ggtggtcgca actttgacga tacgccagtt acgcagaacg tattcgtgga tctctgcttt
    32761 gatccagtgg acggcgaagg agaccaggcg cacacccact tccgggttaa aacggcgcac
    32821 tgctttcatc aggccgatgt taccttcctg aatcaaatcc gcctgtggca ggccatagcc
    32881 cgcataatta cgagcaatat gaacaacaaa ccgcaggtga gacaggatca gcgttttagc
    32941 tgcttccaga tcgccatggt aatgcagctt ttcagccagc gcccgctcct cgtcagccga
    33001 caacatcggc cacgcgttag ctgcccggat gtaggaatcc aggttgccaa ctggggctaa
    33061 agctaaactt tgcattttgt cagtcattca aatcctctca atcgatatct tctggcgctt
    33121 cagtggtagc aacaactgtg ccaaagctta agagcaacga ggttatcatt cactgtttta
    33181 tcagaccgtg attttatcca caagttcaat gcaagcttgt gaataaatta cgcacaaaat
    33241 gtgacataga gatgaaatac cgggaagaga caacggggtc tctttccctg ctacggaacc
    33301 cattgcaggg aaagagtata acacgctttt attattcagg cgtaaagtgg cgtaaatgtt
    33361 gtaccgtggc aagccacgct gccacccagc caatcatcga gcataccagc agcaatagca
    33421 ggcattcatc gaacgataaa ccattgatat caaacttcgt tccgaaaacc tgtgccactt
    33481 ccgccaccgc tgacgacaaa cgcagtacca gaatttctga caaaattaat gacaacaatg
    33541 cgccagaaaa tcccagcagc gcaccgccgt acaggaacgg gcgaaggatg aaaccatccg
    33601 tcgcaccaat cagtttctgc acgttgatgg agtcacggcg agcaaagatg ctcagacgta
    33661 cgctgttgcc gatgacgagg aacactgccg ccaccatcaa cacgccgatc atcgccgaaa
    33721 cgcgcccaac cagcccggtc aacgccgcca gacgggcaaa ccagctgtca tccatccgca
    33781 cttcgtcaat accgttaatc tgcgtgatac gatcacgcag cgtattgagt gattccgtcc
    33841 cctgaaaatc gagtttcggg atcaccaccg ccactgccgg aagcgggttt tcttccagca
    33901 tatccagcgc accaccaaaa ccagaccagt tacggaactc gcccagtgcg tcttcacgag
    33961 aaagatagtt cactttctcc acgccttgct cggcctgcaa ctgtgccacc acgcccgcag
    34021 cggcgtcatc gtccagcgtt ttttgcaagt aaacagtgat ttgcggtgac ggataatact
    34081 gcgtcgccac ctgattaacg tttttgtaca ccatataaca gacgctgggc agcgtcaggg
    34141 agatggcgat aaccatcacc gttaaaaacg tggcgaacgg tttgcttttc agatcctgca
    34201 atgcgccgtg gaaggcatag cgcacctgtt cgttgaaaac gttggttttg cgatttaccg
    34261 gttttggcga ggatttcgcg cgtttcggtg cgttacgacc gccgtcgcct gagccgccga
    34321 ccgatttacg gaagcgatcc agacgcccac caaactgccg aatatgattg attgcatcgc
    34381 gcttattcat ggcccacgcc tccatgcaag tgaccatcgc tcagggtgag catgcgatag
    34441 gaacgccgtg aaatcaggtt aatgtcgtgc gttgccatca atacggttac cccaacgcgg
    34501 ttaaactctt caaacagacg tagaatccct tccgacaacg cgtcgtccag gttaccggtc
    34561 ggttcgtccg ccagcagtac cgcgggctta ttcaccaccg cgcgggcaat gccaacacgc
    34621 tgttgttcac cgccggaaag ctgaataggg aagttcttcg ctttgtccag tagcccgact
    34681 ttatccagtg ccgccgacac ccggcgacga atatcgtcac cgctggcacc agcgataatc
    34741 agcgggatcg ccacgttatc gtagacagta cggtccatca gtagatggtg atcctggaaa
    34801 atcatgccaa tctggcggcg cagaaacgga acttcacggt ttttcagacg cgtgatgtca
    34861 tggccgctaa accagatttt cccggcgctg ggccgctcaa tcccacagat cagcttcagg
    34921 agggtacttt tccctgcgcc ggaatgaccg gtcagaaacg ccatctcacc cggctgcata
    34981 tggaacgtaa cgccctgcag cgcctgtctc ccaccgagat aagccttgct gacatgttca
    35041 aagcgaatca ttgttaatcc tctcgggcaa aaagtgcctc tataaagtcg tccgccttaa
    35101 acggacgcaa atcctcaata cgttcgccga caccaatgta gcggataggg ataccaaact
    35161 ggtcagccac cgagaaaatt accccgcctt tcgccgtgcc gtccagtttc gttagcgtga
    35221 tgccggttaa gccaacagct tcgtggaaca gtttggcctg gcttaccgcg ttctgcccgg
    35281 tgctggcatc aatagtcagc ataacttcat gcggcgcttc aacgtcgagt ttcttcatca
    35341 cgcggacgat tttcttcaac tcttccatca gatgcgattt gttctgcaga cgtccggcgg
    35401 tatcggcaat caggacgtcg atattacgcg ctttagctgc ctgaatggcg tcgaagataa
    35461 cagaggcgga atccgcaccg gtatgctggg caatcaccgg aatattgttg cgctgacccc
    35521 agacctgaag ctgttcaacc gcagctgcac ggaaagtatc gcccgccgcc agcatcaccg
    35581 atttaccctg ctgctcaaac tgacgcgcca gcttaccaat cgtcgtggtt ttacccacac
    35641 cgttgacgcc caccatcagg atcacgaacg gcgttttatc ttcaacattc agcggctcat
    35701 cgactttcgc cagaatctcg cccatctctt ctttcagcag gccatagagc gcctcggcat
    35761 cacgaagctg cttgcgggat gcgccttccg tcagattggt gataatttta cgtgtggttt
    35821 ccacgcccac atcggcgatc aacagctgtt cttccagctc ttcaaacaga tcatcgtcga
    35881 tttttttacc gcggaacagg ctgataaatc cggaaccgag attttctttg gtttttaaca
    35941 ggctgcgttt caggcgcgcg aagaagcctt ctttggtcgg tttttcttgc tcctgggcaa
    36001 tttctgcaac cggctgctct tcttctgccg gaggaactac catcaccgcc tcttctgccg
    36061 cttcggcagc cagcgcctgt gcttccagct cttcgtcggt gatttcttct ttagccgctt
    36121 cttcttctgc cgcttcgaca atctctacgg tttccgcttc agcctgccac tcttctggcg
    36181 aaacctcttc ggcgttgacg tcttccggca acggcagctc ttcacgttcg atagccactg
    36241 gctccggcgt ttcttctacg accggttccg gctgtgcaac aacttccgct tcaggctgcg
    36301 ctttttcact ttcaacaacc tgttcagtga cttccacaac gtcggcagca aaagtttccg
    36361 cctcggcttc agtatgcgcc tgcggctgct cttcaacggc ttgttcagaa gccttcgcag
    36421 gctcttgcgc ctgaacgatt tcttctacaa ccggttgttc attctgaact tctgtctctt
    36481 tttccggggt ctgctctttt tgaccaaagc ccagccagga aaaaaagcca cgtctttttt
    36541 ctttcgccat ttgcgactac actcctcgct gttgcttcat ggcacagcgt taacgctatg
    36601 tacatagcag ctaaaaaaat gatgaaatag tctatcactt aacttaattc acatcaccgc
    36661 ctgcaagtat gtgttatctg gcggattgag caatttatca tgaaaaaacc gaatcattcc
    36721 ggcagcggcc aaatccgcat tattggcggg cagtggcgag gccgtaaact cccggttcct
    36781 gatagcccag gtctgcgccc caccaccgac cgcgtacgcg aaacgttgtt taactggctg
    36841 gctccggtca ttgttgacgc ccaatgtctg gattgcttcg ccgggagcgg cgcgctgggg
    36901 ctggaagcgt tatcgcgcta cgctgcgggg gcaacgttga ttgagatgga tcgcgtggtt
    36961 tctcagcagt taattaagaa tctggcgaca ctaaaagcag gcaatgcacg cgtggtgaac
    37021 agcaacgcaa tgtcattcct ggcgcaaaaa ggcacaccgc ataatatcgt gtttgtcgat
    37081 ccaccgttcc gccgtggctt gttagaagag acgataaatt tactggaaga taacggctgg
    37141 ctggctgacg aagccctgat ttatgtcgaa agcgaagtcg aaaacggtct gcccactgtt
    37201 ccggcaaact ggtcattaca tcgggaaaaa gtggcgggtc aggtggctta tcggctgtat
    37261 caacacgaag cacaaggaga aagtgatgct gattaatatt ggtcgtttgt taatgctctg
    37321 cgtttgggga tttttaatcc tcaatctggt gcatcccttc ccgcgcccgc taaatatttt
    37381 cgttaacgtg gcgctgattt ttaccgtgct gatgcatggt atgcagctgg cgctattgaa
    37441 atccacttta ccgaaagatg gcccgcagat gaccaccgcc gaaaaggtgc ggattttcct
    37501 tttcggcgtg tttgaactgc tggtctggca gaagaaattt aaagttaaaa aataacctta
    37561 ttgttcgcca acaaagctaa caaagcgcgt tcctttatag ctcagcgtac ctttatcgcc
    37621 cactgtcagg gcgtggtact gctgggcgtc gaggcgaaac gtctgctcca ttcctccgct
    37681 ttgcggtttg aagcttgcct catagcgcat actggtgcct gccggagtca cttcctgctg
    37741 gcgcgaacgg cgatcgttaa tcggtttttc ccgcttgttg ctcaccacca ccagcttttg
    37801 ctggagcgga gccatatcat tatcagcttt ttcccgtcgc tgttgcataa aacgaaacga
    37861 tgcggcgacg acaattaagc caatgataac aataaagaaa agaggtggtt tactcatctt
    37921 tatccctcat cggaaaatgc ggaattaagc ataccctgcc agttatggtg ttgtcatccg
    37981 tccaccctcg ccactaaact ggaagcaaga ccgtaggcat tctgcttacg aaaaaataac
    38041 gaattcaagg aactaagatg ctttggtcgt ttatcgctgt ctgtctttcc gcatggctat
    38101 ctgtggatgc atcgtatcgt gggccaacct ggcaacgctg ggtgtttaaa ccgttaaccc
    38161 ttcttctcct gctgttactg gcctggcaag caccgatgtt cgacgccatt agctatctgg
    38221 tgctggcggg actgtgcgcc tcactgctgg gcgatgcgct aaccctgttg ccacgtcaac
    38281 gtctgatgta cgccatcggc gcgtttttcc tctcgcacct gctgtacacc atctatttcg
    38341 ccagtcagat gacgctctct ttcttctggc ctctgccact ggtgttgctg gttctgggtg
    38401 cgctgctact ggcgattatc tggacgcggc tggaagagta ccgttggcct atctgcacgt
    38461 ttatcggcat gacgctggtg atggtgtggc tggcaggtga actgtggttc ttccgtccga
    38521 ccgctccggc gctctctgcg tttgttggcg cttcgttgct gtttatcagt aactttgtct
    38581 ggctggggag ccactatcgc cgacgcttcc gtgcggataa cgcgattgct gcggcctgct
    38641 actttgccgg tcacttcctc atcgtccgct cactgtatct ctgataaaac ttgactctgg
    38701 agtcgactcc agagtgtatc cttcggttaa tgagaaaaaa cttaaccgga ggatgccatg
    38761 tcgacacctg acaatcacgg caagaaagcc cctcaatttg ctgcgttcaa accgctaacc
    38821 acggtacaga acgccaacga ctgttgctgc gacggcgcat gctccagccc gccaacgctc
    38881 tctgaaaacg tctccggcac ccgctatagc tggaaagtca gcggcatgga ctgcgccgcc
    38941 tgtgcgcgca aggtagaaaa tgccgtgcgc cagcttgcag gcgtgaatca ggtgcaggtg
    39001 ttgttcgcca ccgaaaaact ggtggttgat gccgacaatg acatccgtgc ccaggttgaa
    39061 tctgcggtgc aaaaagcggg ctattccctg cgcgatgagc aggccgccga cgagccacaa
    39121 gcatcgcgcc tgaaagagaa tctgccgctg attacgctta tcgtgatgat ggcaatcagc
    39181 tggggtctgg agcagttcaa tcatccgttc gggcaactgg cgtttatcgc gaccacgcta
    39241 gttgggctgt acccgattgc tcgtcaggca ttacggctga tcaaatccgg cagctacttc
    39301 gccattgaaa ccttaatgag cgtagccgct attggtgcac tgtttattgg cgcaacggct
    39361 gaagctgcga tggtgttgct gctgtttttg attggtgaac gactggaagg ctgggccgcc
    39421 agccgcgcgc gtcagggcgt tagcgcgtta atggcgctga aaccagaaac cgccacgcgc
    39481 ctgcgtaacg gtgagcggga agaggtggcg attaacagcc tgcggcctgg cgatgtgatt
    39541 gaagtcgccg caggtgggcg tttgcctgcc gacggtaaac tgctctcacc gtttgccagt
    39601 tttgatgaaa gcgccctaac cggagaatcc attccggtgg agcgcgcgac gggcgataaa
    39661 gttcctgcag gagccaccag cgtagaccgt ctggtaacgc tggaagtgct gtcagaaccg
    39721 ggtgccagcg ccattgaccg gattctgaaa ctgatcgaag aagccgaaga gcgccgcgcc
    39781 cccattgagc ggtttatcga ccgtttcagc cgtatttata cgccagcgat tatggccgtc
    39841 gccctgctgg taacgctagt gccgccgctg ctgtttgccg ccagctggca ggagtggatt
    39901 tataaaggtc tgacgctgct gctgattggt tgcccgtgtg cgttagttat ctcaacgcct
    39961 gcggcgataa cctccgggct ggcagcagca gcgcgtcgtg gggcgttaat taaaggcggt
    40021 gcggcgctgg aacaactagg tcgtgttacc caagtggcgt ttgataaaac cggtacgctg
    40081 accgtcggta aaccgcgcgt taccgcgatt catccggcaa cgggtattag tgaatctgaa
    40141 ctgctgacac tggcggcggc ggtcgagcaa ggcgcgacgc atccactggc gcaagccatc
    40201 gtacgcgaag cacaggttgc tgaactcgcc attcccaccg ccgaatcaca gcgggcactg
    40261 gtcgggtctg gcattgaagc gcaggttaat ggtgagcgcg tgttgatatg cgctgccggg
    40321 aaacatcccg ctgatgcatt tgctggtttg attaatgaac tggaaagcgc cgggcaaacg
    40381 gttgtgctgg tagtacgtaa tgatgacgtg ctgggtatca ttgcattgca ggataccctg
    40441 cgcgccgacg ctacaactgc catcagtgaa ctgaacgcgc tgggggttaa aggagtgatc
    40501 ctcaccggcg ataatccacg cgcagcggcg gcaattgccg gggagctggg gctggagttt
    40561 aaagcgggcc tattgccgga agataaagtc aaagcggtga ccaagctgaa tcaacatgcg
    40621 cccctggcga tggtcggtga tggtattaac gacgcgccag cgatgaaagc tgccgccatc
    40681 gggattgcta tgggtagcgg cacagacgtg gcgctggaaa ccgccgacgc agcattaacc
    40741 cataaccacc tgcgcggcct ggtgcaaatg attgaactgg cacgcgccac tcacgccaat
    40801 atccgccaga acatcaccat tgcgctggga ctgaaaggga tcttcctcgt caccacgctt
    40861 ttagggatga ccgggttgtg gctggcagtg ctggcagata cgggtgcgac ggtgctggtg
    40921 acggcgaatg cgttaaggtt gttgcgcagg agataaggca aaccgatcgc aacattgagc
    40981 gcgatcggtc ccctcgcccc tctggggaga gggttagggg gtgaggaaaa ggcggcatcg
    41041 aagccaatca gcccctatca accgccttta cgaatcaaat aacgataagg cagtccatcc
    41101 gtctctttcg caaccagttc gtgttccata aaggtacaaa acccaggaat atcgcgggta
    41161 gtggccggat cgtcggcgat aatcagcaac gtttcgccag gctgcatatt gcgcacggtt
    41221 ttgcgcacca tcatcaccgg ttccgggcag cgcaggccaa gcgcgtcgag tgtgtggtca
    41281 gggctggaaa agagatcggt cattttcttc tcatcactta aaaaacggcg ctagtttacg
    41341 ccctgtgagt ccgtaagcaa accaggttaa cgattgcgtg aaaattaacc attgcattgt
    41401 caacgtaaag cagtatcatg cggcggctcg aaaaaagggt aagcacgtta ttatgttaag
    41461 gtaacagacg tgtcgtacgt attgggttcc ctcaccccaa tggttaatca aaaaggtaca
    41521 atatgaacgt tttctcgcaa actcaacgct ataaggcgtt gttctggtta tcgttatttc
    41581 atcttctggt gatcacctcc agtaactatc tggttcagct tcccgtctcc atttttggtt
    41641 tccataccac ctggggcgcg tttagctttc cgtttatttt tcttgctacc gacctgaccg
    41701 tgcgtatttt tggcgcaccg ctggcccgac gcattatctt cgcggtaatg atccctgcgt
    41761 tattaatctc ctacgtcatc tcgtcgctat tctatatggg ttcctggcag ggattcggcg
    41821 cactcgccca cttcaacctg tttgtcgccc gtatcgccac cgccagtttc atggcctacg
    41881 cgctggggca aatcctcgac gtgcacgttt ttaaccgcct gcgtcagagt cgccgctggt
    41941 ggctggcacc gacagcgtcc acactgttcg gtaacgtcag cgacacgctg gcctttttct
    42001 tcattgcctt ctggcgtagc ccggatgcct ttatggctga acactggatg gaaatcgcgc
    42061 tggtcgatta ctgtttcaaa gtgttaatca gtatcgtttt cttcctgcca atgtatggcg
    42121 tattactcaa tatgctgttg aaaagactgg cagataaatc cgaaatcaac gctttgcagg
    42181 cgagttaaag gttcgttatc agagttgtga taagatggat gaatgagccg ttatggccgt
    42241 ttatcgaaag gaagaagtca atgcgcaatc tggttaaata tgtcggaatt ggcctgctgg
    42301 ttatggggct tgcggcctgt gatgataaag acactaacgc tacggcgcag ggttcggttg
    42361 cggaaagtaa cgctaccggg aatcccgtca acctgcttga tggcaagtta agtttctcgc
    42421 tgccagcgga tatgaccgac cagagcggta agctgggaac gcaggccaat aacatgcatg
    42481 tctggtccga cgccaccggg cagaaagcag tcatcgtcat catgggcgat gatccgaaag
    42541 aagatctggc ggtgttggcg aagcgtctgg aagatcagca acgtagccgc gatccgcagc
    42601 tgcaagtggt aaccaataaa gccattgagc tgaaaggtca caaaatgcag cagttagaca
    42661 gtattatctc cgcgaaaggc cagacggcgt actcttccgt tattctgggt aacgtgggta
    42721 atcaactgct gaccatgcaa attacgctgc cagctgacga tcagcaaaaa gcgcaaacca
    42781 ccgcagaaaa catcattaat acgctggtta ttcagtaagt tttaagatga tgaggcggcc
    42841 tcagggacgt gttccggagg ccgttttttt aatcgccacg tcagtaataa cgcgattgcg
    42901 accagtcccg ccgccgccag ataaatcacc ggtacgcccg cccagctcat caccagccca
    42961 gccagtggtc cagtcacgcc aagcgataaa tccataaata cggtgtaagt tgccagcgcc
    43021 gccccctgat tttgctgcgg aaccgctttt accgccacta cacccaatgc cgggaacacc
    43081 agcgaaaacc cggcccccgc cagtaagacg ccgattttcg ccatccacgg catagtcgcc
    43141 acgccaacca gtagcaggcc gattatctca acgctaaagc aaatcatcgc tacgtttaag
    43201 ccaccgatac ggttaatgcc gttagggaat aacaaacgcg taccgacaaa cgcacagcta
    43261 aacagcgtca gcgcgaaagc cgcaccgtcc caacctttag cgtcataaaa cagcgtgata
    43321 aaggtggcga tgacgccaaa tccggcggaa gccagtgcca gcgccatacc gtacagccag
    43381 acgcgcccaa gcaccgcgcg aaacggcagc ggtttgcctt tactggcttt taccgtcgga
    43441 cgcgggatcg ccaacaaaat ggccaccagc gccacgccca taatgattaa cgctaacgcc
    43501 tgcaagccgc cccagtgata aaacacgacg cctaacggcg cacccatcgc catcgccccg
    43561 taagtgacaa tgccgttcca cgaaatcacc cgcccgatat gcagcgagcc aaccactcca
    43621 acgccccata gggtcgatcc cgttccggca aaactttgcc caatcccaag gatgacgcgc
    43681 cccaggcaaa gtaataacag gctgatgaca ggcagactgg cggttaatcc tgccgtcaga
    43741 taccccagac cgctcaaaaa gcagccgcat aaaccgaaga cgacaatctt tttgggtccc
    43801 agcaaatcgg cgtaacgtcc ggcatgaggg cggctcagca aggtggcgaa atattgcagg
    43861 ctgataacca atcctgccca gaaggcgcta aagcccatca catcatggac atagcccggt
    43921 aatacagcga gcggcaaccc gatggtgagg tagctggcga agttaaacat gacaatggag
    43981 acaatgcgca aattcaggcg caatccgttt agcgcgggtt cggctacggg ttcgggcatg
    44041 aggatcacca catttttaca acagtgtttc atttttacca tgtgctgacg tgaaaatcag
    44101 cagtaagaat cagaatattg ctggcgtgac tcccgcgcta cacttaacac aaaaagtcac
    44161 aaggaagccc caatggaaac ccctcaaccc gataaaacgg gcatgcacat tctgctcaag
    44221 ttggcctcgc tggtagtgat cctcgcgggc attcacgcag cggcagatat cattgtgcag
    44281 ctgttactgg cgctgttttt tgccatcgtc ctcaacccgc tcgttacctg gtttattcgt
    44341 cggggagtac aacgccccgt tgccattacg attgtagtgg tggtgatgct gatcgcacta
    44401 accgcgctgg tcggcgtact ggcggcatcg tttaacgaat ttatctctat gctgccgaag
    44461 tttaataagg agctgacgcg caaacttttt aaattgcagg agatgttgcc ttttcttaat
    44521 ttgcatatgt cgccggagcg aatgctgcag cggatggact cggaaaaagt ggttaccttc
    44581 accacagcgc taatgaccgg gctttccggg gcaatggcga gcgtgctttt gctggtgatg
    44641 accgtagttt ttatgctgtt tgaagtgcgc cacgtccctt acaaaatgcg ttttgcgctg
    44701 aataacccac agattcacat cgcaggatta caccgcgcac ttaaaggcgt ttcgcattat
    44761 ctggcgttaa agacgctact cagtttatgg acaggtgtca tcgtctggct ggggctggcg
    44821 ctaatgggcg tacagtttgc gctgatgtgg gcagtactgg cgtttttgct caactacgtg
    44881 cccaatatcg gcgcggtaat ttccgccgta ccgccaatga ttcaggtgct gctgtttaat
    44941 ggtgtttacg aatgtattct ggtcggcgca ttgtttttag tggtccatat ggtcatcggc
    45001 aatattttag aaccacggat gatgggccat cgcctgggga tgtccaccat ggtggtattt
    45061 ctttcattgt taatctgggg atggctgctc ggcccggtag ggatgctact ttcggtgcca
    45121 ttaaccagcg tgtgtaaaat ctggatggaa acgacgaaag gtggcagcaa actggcgatt
    45181 ttactggggc cgggcagacc gaaaagtcgg ttaccgggat gaggcgacaa gtgatacgat
    45241 acgcactttc attttccatt aaacgttggc cctgatatgt atcggatagt tctggggaaa
    45301 gtttcgacct taagcgcagc tccactgcca ccgggtttac gcgagcaagc accgcaaggt
    45361 ccacgacgcg aacgctggct ggcggggcgt gcattgcttt cgcacacgct ttccccgcta
    45421 ccggagatca tctatggcga acaaggcaaa cctgcattcg cgccggaaac gccgctatgg
    45481 ttcaacttaa gccatagcgg tgacgatatc gctctgctgt tgagcgatga aggcgaagtc
    45541 ggctgcgata tcgaagtgat tcgcccgcgc gccaactggc gctggctggc gaacgccgtg
    45601 ttcagcctcg gggaacacgc tgaaatggac gccgtgcatc ctgatcagca actggaaatg
    45661 ttctggcgca tctggacgcg caaagaagcc atcgttaaac agcgtggcgg cagcgcctgg
    45721 caaatcgtca gcgtagatag cacctatcac tcctcgctgt cagtcagcca ttgtcagcta
    45781 gaaaatttaa gccttgcgat ctgcaccccc acccccttta cgctcaccgc cgacagtgtg
    45841 caatggatcg attcagttaa ctgatccgcc cacccgactg cccatctatt gatccagaac
    45901 aggtaatcag tatgacgaat acttaaaatc gtcatactta tttccgccat ctattttaat
    45961 ccattggggt taccatgctc tccacactcc gccgcactct atttgcgctg ctggcttgtg
    46021 cgtcttttat cgtccatgcc gctgcaccag atgaaatcac caccgcctgg ccggtgaatg
    46081 tcgggccact aaacccgcac ctttacacgc ctaaccagat gttcgcccag agcatggttt
    46141 atgaaccatt ggtgaaatat caggcagacg gttcggtgat cccgtggctg gcaaaaagct
    46201 ggactcattc agaagatggt aaaacctgga ccttcaccct gcgtgatgac gtgaaattct
    46261 ccaacggtga accgttcgat gccgaggcgg cggcagaaaa cttccgcgca gtgctcgata
    46321 accgtcaacg tcacgcctgg ctggagctgg caaaccagat tgttgatgtt aaagcactca
    46381 ataaaacaga gctgcaaatt accctgaaaa gcgcctacta tcctttcctg caagaactgg
    46441 ccctgccccg tcctttccgt tttatcgctc cctcgcagtt taaaaaccat gaaaccatga
    46501 acggaattaa agcgccgatt ggcaccggac cgtggatttt gcaggaatcg aaactgaatc
    46561 agtacgatgt cttcgtccgt aacgaaaact actggggcga aaagccagcg attaaaaaga
    46621 tcacctttaa cgtcatcccg gacccgacta cccgcgcggt ggcgtttgaa actggcgata
    46681 tcgacctgct gtacggaaac gaagggttat taccgctcga taccttcgcc cgctttagcc
    46741 agaatccggc ttaccacacc caactgtcac agccgatcga aaccgtgatg ctggcgctca
    46801 ataccgccaa agcccccacc aacgagctgg cagtacgtga agctcttaat tacgcggtaa
    46861 acaaaaaatc gctgattgat aacgcgttat atggcaccca gcaggtcgcc gacaccctgt
    46921 ttgccccttc tgtgccttac gctaatctcg gcctgaaacc gcgccagtac gatccgcaga
    46981 aagcgaaaga gttgctggaa aaagccggtt ggacgctgcc tgcgggcaaa gacatccgcg
    47041 agaaaaatgg tcagccgctg cgcattgaac tgtcgttcat cggcaccgat gcgttaagca
    47101 aatcgatggc ggaaatcatt caggctgata tgcgccagat tggcgcagat gtctcgctga
    47161 ttggcgaaga agagagcagt atctatgctc gtcagcgcga cggtcgtttt ggcatgattt
    47221 tccaccgcac ctggggcgcg ccatacgatc cacacgcctt cctgagttca atgcgcgtac
    47281 cgtcgcacgc tgacttccag gcacagcaag gattagccga caagccgctg attgataaag
    47341 agatcggcga agtgccggcg acccatgacg aaacgcaacg tcaggcgctg tatcgcgaca
    47401 ttctgacccg tctgcatgac gaggcggttt atctgcctat cagctacatc tcgatgatgg
    47461 tggtatcaaa accggagctg ggtaatatcc cctacgcgcc gatcgccacc gaaattccgt
    47521 tcgaacagat taaaccggtg aaaccttaat gttgcgttac gtattacgcc gctttctgct
    47581 gctgatcccg atggtgctcg ccgcctcggt gatcattttt ctgatgctgc gcctcggtac
    47641 cggcgacccg gcgctcgatt atctgcgttt atctaacctg ccgccgacgc cggagatgct
    47701 ggcctctacc cgcaccatgc tcgggctgga tcagccgctg tacgtccagt acggtacctg
    47761 gttgtggaag gcgctgcatc ttgactttgg tatctcattc gccagccaac gcccggtact
    47821 ggacgatatg ctgaacttcc tgcccgccac gctggaactt gcaggtgcgg cgctggtatt
    47881 aattctgctc acttccgtac cgctcggtat ctgggcggcg cgccaccgcg atcgtttgcc
    47941 ggatttcgcc gtgcgcttta tcgcgtttct tggcgtgtcg atgcccaact tctggctggc
    48001 atttttgcta gtgatggcgt tttcggtgta tctgcaatgg ctacccgcga tgggctacgg
    48061 cagctggcag cacatcattt tacctgcggt ttccatcgcc tttatgtcgc tggcgattaa
    48121 cgcgcgactg ctgcgcgcca gtatgctgga cgtcgccggt cagcgtcacg tcacctgggc
    48181 gcggctacgc ggcctgaatg acaaacagac cgaacgccgc catattctgc gcaatgcctc
    48241 gctgccgatg atcaccgccg tggggatgca cattggcgaa ttgattggcg ggacgatgat
    48301 tatcgaaaac atctttgcct ggcctggcgt cgggcgctat gcggtgtcgg cgatttttaa
    48361 ccgtgactat ccggtgattc agtgctttac gctgatgatg gtggtggttt ttgtggtctg
    48421 taatttgatt gtcgatttgc tcaacgccgc gctggacccg cgcattcgtc gtcatgaagg
    48481 agcgcacgcg tgaacttttt cctctcttcc cgctggtcgg tacgcctagc gctgatcatt
    48541 atcgccctgc tggcgctgat tgcgctcacc agccagtggt ggcagccgta cgatccacag
    48601 gcaattgatt tgccgtcgtg cctgctttcg ccggatgcgc agcactggct gggcaccgat
    48661 cacttaggtc gcgatatttt ctcgcggctg atggcagcga cccgcgtgtc gctcggttcg
    48721 gtaatggcct gcctgctgct ggtgctgaca ttagggctgg ttattggcgg cagcgccggg
    48781 ttgattggcg ggcgcgttga tcaggccacc atgcgcgtcg ccgatatgtt tatgaccttc
    48841 ccgacctcga ttctgtcgtt ctttatggtt ggcgtgctcg gcaccgggct gaccaacgta
    48901 attatcgcca tcgccctgtc gcactgggcg tggtatgcac gcatggtgcg cagcttggtg
    48961 atttcactac gccaacgcga gtttgtgctg gcgtcacggc tttccggtgc gggccatgtg
    49021 cgggtgtttg tcgatcatct ggcaggcgcg gtgatccctt cgctgctggt gctggcaacg
    49081 ctggatatcg gccatatgat gctgcacgtc gcggggatgt ctttccttgg cctcggtgtg
    49141 accgcgccga ccgccgaatg gggcgtgatg attaacgacg cgcgccagta tatctggacc
    49201 cagccgctgc aaatgttctg gccggggctg gcgctgttta tcagcgtgat ggcctttaac
    49261 ctggtgggtg acgcactgcg cgatcatctg gatcctcatc tggtgacgga gcacgcacac
    49321 taatgccgca acagattgaa ctacgtaata tcgcgctaca ggccgcgcag ccgctggtac
    49381 acggtgtatc gttaaccctg caacgcgggc gcgtgctggc gttagtcggc ggtagcggca
    49441 gcgggaaatc attaacctgc gccgcgacgc tgggcatttt gcccgctggc gttcgccaga
    49501 cggcggggga aattttagcc gatggcaaac cggtttcgcc ttacgccctg cgcggcatca
    49561 aaattgccac catcatgcag aacccgcgca gcgcctttaa tccactgcac accatgcaca
    49621 cccacgcgcg ggaaacctgc ctggcgttag ggaaacccgc cgatgacgct acgcttaccg
    49681 ctgccataga agcggtgggg ctggaaaacg ccgcgcgcgt gctgaagctg tacccgttcg
    49741 agatgagcgg cggcatgttg cagcgcatga tgattgcgat ggcggtgctg tgtgaatcac
    49801 cgtttatcat cgccgatgaa ccgaccaccg acctcgacgt ggtagcacag gcgcgcatcc
    49861 tcgatctgct ggaaagcatt atgcaaaaac aagcgccggg aatgctgctg gtcacccatg
    49921 atatgggcgt tgtggcgcgt ctggcggatg acgtggcggt gatgtcacaa ggtaaaattg
    49981 tcgaacaggg cgatgtagaa acgctgttta acgcccccaa acatacggtg acgcgcagcc
    50041 tggtttccgc tcatctcgcc ctctacggta tggagctggc atcatgacct tacttaacgt
    50101 ctgcggcctt tcccatcact atgcccacgg tggatttagc ggaaaacatc aacatcaggc
    50161 ggtgctgaat aacgtttccc tgaccctgaa aagcggcgaa accgtcgccc tgctgggacg
    50221 cagcggctgc ggaaaaagca ccctcgcgcg gctgctggta ggcttagagt ctccctcaca
    50281 agggaatatt agctggcacg gcgaatcgct ggcgaaactt aatcgcgccc aacgtaaagc
    50341 cttccgccgt gatattcaga tggtgtttca ggactccatc agtgccgtga atccgcgcaa
    50401 aaccgtgcgc gagatcctgc gtgaaccgat gcgccacctg ctatcactga aaaaatccga
    50461 acaactggcg cgcgccagcg aaatgctgaa ggcggtcgat ctcgatgaca gcgttctcga
    50521 caaacgccca ccgcagttaa gcggcggcca gctccagctc gtctgcctgg cacgcgcgct
    50581 ggcggtcgaa ccgaaattgc tgattctgga tgaagccgtt tctaaccttg atctcgtgtt
    50641 acaggcgggc gtcattcgcc tgctgaaaaa gctacaacaa cagtttggca ctgcctgcct
    50701 gttcatcact catgacttac gcctggtgga acgcttttgc cagcgagtga tggtgatgga
    50761 caacggacaa atcgccgaaa cccaggcggt gggagataaa ttaacctttt cctctgacgc
    50821 cggacgtgtg ctacaaaacg cggtattacc cgcattcccc gtgcgccgtc gcacctcaga
    50881 aaaggtttaa cgccaatgca acgagtcacc atcacgcttg atgacgattt actgaagacg
    50941 ctggacagcc tgagccagcg tcgtggttat aacaaccgtt ccgaagctat ccgcgatatt
    51001 ctgcgtagtg ccctggcgca ggaagctacc cagcagcacg gcacacaagg tttcgcggtg
    51061 ctgtcgtatg tgtatgaaca cgaaaaacgc gacttagcca gccgtattgt ttccacccag
    51121 catcatcacc acgacctctc cgtcgccacg ctacatgtgc acatcaacca cgacgactgt
    51181 ctggaaatcg ccgtgttgaa aggtgacatg ggtgacgtgc agcattttgc cgatgacgtt
    51241 atcgcccagc gcggcgtgcg gcacgggcat ttgcagtgcc tgccgaagga agattgagtt
    51301 atttctaaaa caaggaaaga aaagatatta actgaactta atttataaaa ttaagttcag
    51361 ttgcatagat agatcctgta aaggcagata ttctgcgtaa tgtacgtcgc taaaatttat
    51421 aaatgattca aacgctcatt cagtgtttca ataatatagg tattaacaga gacttgttgc
    51481 tgcgcagccg cattattaag gcgttcactc agcgattctg gatagcgtaa cgtaaaggtc
    51541 ttaatctttt ctgtacgcac atacggctca atacccgcag ctttacaatc ctcaagatac
    51601 tctcgcaggg aaagctctcc ttctttttgc agcccctgaa tactgtcaga gacaaaatca
    51661 caataacctg acaagcccag gaatttgcca cgaaatgcat tcagttctgg cacgtaggta
    51721 ataacagctg gttgcccggc aatctccata gagtttggcg tcttcagttt gatcatggtt
    51781 ttacccctat acttagtaac cagtcacaca cgctttctac agcgccttta tcggtatctg
    51841 gcgacggatg aggccgatgg aaacacgcaa cgctcatatt tagtatgaac ttgcaacgcg
    51901 aacctcttcc ttccttaatt tctccgccta atgctttaac cagtgactct atatcagacc
    51961 acttgatccc ttgaggaaca ggagttttaa atatttgatc caatgtattt ttttgtttct
    52021 tccgtaaagc ctgtaccttg gttctcatgg catgtcctta tgacttcact ttatgacatc
    52081 acagtatgaa gtcaagtttc ccttccttac gccattgtcc caatcgtctt cctgaatcgc
    52141 agcagtgcga tggtgaaaaa cgcgccgcca attgccatca gcgtcaggaa ctgcggccag
    52201 acgatttcga atccggcacc ccggtagagg atggcctgcg cgaggctaac aaagtgtgtc
    52261 gtcggcatgg tcagcataat gtcctgcacc atctgcggca tactttcgcg cggcgtggat
    52321 ccaccggaaa gcatttgcag cggcagcagc accagaatca ccagcagccc cagttgcggc
    52381 attgaacgcg ctatcgtccc cataaaaatg ccgattgacg tggtggcaaa cagactgagc
    52441 gccacgccca gcataaacag cgggatcgag ccttcaatcg gtacgcccag tacacctttc
    52501 accatcagca ccagcgataa tcccgatacc accagcacca ccagccccat cgaccagatc
    52561 ttcgccatca tgatctcaaa cggcgttatc ggcatcacca gtaaatgttc caccgtgccg
    52621 tgttcacgct cgcggatcag cgccgatccg gtcaatacaa tcgccagcat ggtaatgttg
    52681 ttgatgatcg ccatcacccc gccaaaccac gcgggatcaa ggttcgggtt aaagcgcatc
    52741 cgggtttcca gcgataccaa cggttcgctg ttatcacggt agcgcgcgac aaagctgttc
    52801 acttcaccgt tgataatatt ctggatatac ccattgccgg taaatgcctg gctcatgcgc
    52861 gtggcatcga cgttcacctg aatatccggc tggcgtccgg cgaggacatc acgctgaaaa
    52921 ttaggcggaa tatttatcgc gaaggtatag cgtccggcgt ccagtccggc atccatctca
    52981 tcggcggtga tcatctccgg tggcaaaaac cacggacgat agaagctgtt aacgatccgg
    53041 ttcgataact gcgattgatc catatcggca atggcgatcg gcgcgaggtt caacgatcct
    53101 ggcgtaacgg tcgctgacga atacaccgac accgtaaacg agaagacaat cagcgtcagc
    53161 atcgctttat caccgagcag actgcgcaac tctttgatac ccagattaaa aatattgcgt
    53221 aaatggcgca tcatccctcc tgttttttca gcagcaggat gcttaagccc atcaccagcg
    53281 ggatggctat cagcaacggg ataaaaagtt gccacaaatc agtcagatcc agcgctttcg
    53341 agaacgttcc gcgggcgata gtcagaaaat gactggtcgg gtaaacctcg ccgatccaac
    53401 gtccaggccc ttccagcgaa gctaccggat cgatcatccc ggaaaactgt gtcgccggga
    53461 tcaatgtgat aatcgccgtt ccgaaaatgg cggcaatctg gcttttcata aaggtggaga
    53521 tcagcagccc cattccagtg gcaatgatga tatacagcag cgccgccaag gtgagcgtca
    53581 ggaaactgcc tttatgcggt acgccaaaca caaacaccga caggccgcag agcaggaaaa
    53641 agttcagcat ccccagcgcg atgtacggca gctgtttgcc aagcaaaaat tcgctacggg
    53701 tggtgggggt cacgtaaagg ttgataatcg accctagctc tttttcccgc accacgctaa
    53761 gggcgcttag cattgacggg atcatcatca gcagaagcgg gatcaccgcc ggaacaatcg
    53821 ctggcaggct ttttacgtcc gggttatagc gataacgcgt ctcaatgttc atcagcccgc
    53881 tttggctggc gggtgtcgat tgtcggctcg ccacatcctg aagccaactc tggtgcattg
    53941 cctgcacgta accttttacc gtttcggcac gactgggcat cgctccgtcg atccagacgc
    54001 cgagttccac aggcgtacca cgcgcgatat cgcgcccgaa attgggcggg atctcaatgg
    54061 ccaccgtgat atcgcccgca cgcatccgac gatcaagctc gtcataactg gtgagcggcg
    54121 gctgttcgat aaagtaacgg gaaccggaga ggttgagtgt ccacgcctgg ctactgacgg
    54181 tctggtcgcg gtcgagcacc gcaaagcgca ggttttccac atccatactg atgccgtaac
    54241 ccattatcag catcaggatc accgttccca tcagcgccag cgtcgaacgt actggatcgc
    54301 gtcgcagttc cagcgcttcg cggcggctgt agctaaacag acggcgcagg ctaaatccct
    54361 gacgcggcgc gtgggtggtg tcgtgtacca cgggcggcgc ttcggcttcg ttgctctgcc
    54421 ctgccgcttc ctgcaaatag gcgataaatg cctcttccag actggcggct ccgcgtttct
    54481 caaccagttc ctgcggtgta ccgctggcaa gcacttttcc ggcgtgcatc agtgagatgc
    54541 ggtcgcaacg ttccgcttcg ttcataaagt gggtggagat gaagatagtc actttgtcct
    54601 ggcgcgagag atcgaccatc aactgccaga acatatccct cgccaccgga tcgacaccag
    54661 aagtaggctc atcgaggatt aacatctccg ggcgatgaat caccgccacc gccagcgaaa
    54721 gccgctggcg aatgccgagc ggcaatgact ccggcagaac atcttcaacg tcgttgagct
    54781 taaaacgctc gctcatttca gccactcgcg cgggaatttc cgcttccggg atgtgaaaca
    54841 aacgggcatg taactccagg ttttgccgca cggtgagttc gttatagagc gaaaacgcct
    54901 gcgacatata gcccacacga cggcgggtat cgatatcttt tggatcaacc ggttgcccaa
    54961 atagccacgc ctcaccttcg ctggcgggca gcagtccggt aagcattttc atggtggtgg
    55021 atttaccgca gccgttcgaa ccaaggaatc caaaaatctc cccgcgtgga atgcggaaat
    55081 tgacgtgatc aacggcaacg aaggaaccaa aacgcatggt cagatcgcgc gcttcgatgg
    55141 caatctctgc gttttcaggt tgatacggtg ggatcactac cgcctgatgc gcctggcgtt
    55201 gcgcttgcgg taacagatta ataaatgctt cttccagcgt ggcgctttgc gtttgctgcc
    55261 gcagctcttc ggcgctgccg gttgccagca cttctccggc attcatcgct accagccagt
    55321 cgaagcgttc ggcctcttcc atataggcgg tggcgaccag cacgctcata ttgctctgcc
    55381 gctggcgaat actgtcgatc agatcccaga actgggcgcg ggagagcggg tcaacccccg
    55441 ttgttggctc atcaaggatc aacagttccg ggtcgtggat taacgcgcag cacagcccaa
    55501 gtttttgctt catcccgccg gagagtttac ctgccggacg atcgcgaaac ggtgctaacc
    55561 cggtgctggt cagcagctca ttaattcgca cttcccgctc cgctttatcg tgaccgaaca
    55621 ggcgagcgaa aaaatcgaca ttttcataca ccgacaaggt gtggtagagg tttttgccca
    55681 gcccctgcgg catccaggcg atgcgcgggc agacgtctcg gcgatgcttc gggtcgtgca
    55741 tatcgccacc cagcaccacc acattgccct gctcaatgac gcgggcaccg gaaatcagtg
    55801 acaataaact cgacttcccg acgccatccg gaccaatcag cccgaccatg caacgggccg
    55861 gaatatcgag agtgatattg ttcagcgcaa cggtttttcc ataatgctgg ctcacgcccg
    55921 ccagttgcgc gacaggcggg acgggaacca gttccagatg cgtcattgcg gcaacctcac
    55981 cacgaggtcg tcaggccacg gaagttcttc attcacccgc acccacgcta cgcccggtaa
    56041 accggttttg acatattcca gatgctgctg gagtaattcc ggtgggatac gcgctttgac
    56101 gcggaacatc agtttcagcc gttcatcgct ggtttcgacg gtttttggcg tgaactgggc
    56161 gacactggcg acaaaactga tggttgcagg aatacgcaga tctggcgcgg catcgaggat
    56221 cagccgggct tcaccgccca gtttcagcgt gcccgcctgt tcggttggca ggaagaaggt
    56281 catatagacg tcgctgagat cgaccatatt cagcacccga ccgcctgccg ccagcacttc
    56341 ccctggctcg gcaacccgat actgcacgcg tccgtcacgc ggggctttca gttcactgtc
    56401 atcgatatct gcggcaatgc gccgttcagt ggcttgcgct gcttcgaccc gagtttgcgc
    56461 ctgaatgata ttggtgcgtg ctgcttctat agccgcttta gaagccgaaa cctgggcttt
    56521 cgctgattcc agcgcagctc gggcgctctc agcggcggcg cggtcgtcat ccagctgttg
    56581 cgcagaaata gcccctcgtt gggcgagcga gcgggaacgc gtatgacgtt tggcaacaga
    56641 gtccagttcg gcctggcgtt gattgaccag cgactgtgcg gcacgagttt cactttgtcg
    56701 ttgctccagc aaagcctgcg cggcggcaac ggcgctttgt gcctctttga tttgcgcgat
    56761 ggcttccagt cgctgttcct gcaacacgcg agtatccatc ttcgccagca cttcgccttc
    56821 gcgaacaaac tggccttctt tcaccagaat ggtgtcgata cgcccggcaa ttttgctggc
    56881 gatatccact tccgtcgctt caatgcgccc attgctgaca gcaaagcctt ccggcacacc
    56941 cgccgggcgc aacagccacc acgccacgac ggcagccacc gccagtgccc cgacaaccca
    57001 ccacgccaga tggcgcttac tcttatccat aatcgacccg ccataatccc tgtaagcaaa
    57061 cgacgcgtgt tacaactgcc gccgaacgac gttcagcgga ttcttccatc cgggataaga
    57121 aggaaacgac aaatacgacc agcgcgcaga gcattgagcg ccaacgggga aggcttagtg
    57181 aaaacgggta gtaatgttgt catcatcagc attcctggcc gtaaatgaaa cacgcatact
    57241 cataagtgtg atctataaaa ctggctgaca aaacggtatc catcctgttg gatcgcattt
    57301 cacgcctgtt cagttgcagg aacagagcat tcctttttac gttttatcaa cagtctcaac
    57361 cagatagtaa cattaatatt catgttgtct atggttcaga gctgtaacat tgtgcgcctt
    57421 ctaatcaaag gcataagtcc atttctgttt ttttcatgct ggtaagactt ccagggagtg
    57481 gttatctctg gccctgcacc agggttaatt tggcaattat gcatgctgaa cattcttcca
    57541 gcatgtttac tgtcacgggc gttaagacct aaaactgtcc ccgtttcacc tatgcgttct
    57601 ctcccaccat ttccgttatg tgcaacaact tgatttatac ctaacatttg tgaacatgct
    57661 aatagtgctt caagtttagg tctgtaatct gtcgaaacag aaaaataacg attaaaggat
    57721 cgacaagaga tagcccggct agttttaaac tttttgccat taattatagc atgatgcttt
    57781 ttattaataa tttccgctaa ttcgaccgga tcgagagcat tggtcatttc atcaatagag
    57841 acagttatcg gtcctataat gtaacgtttt cgctgatcat caaaagttat cccatgatga
    57901 tttgccatta ttttgtattt ggggtcatag aaacatacag gataatcttt tatcccgtcg
    57961 taggtatcct catcaagtgg tttcatgcgc gtgaagtttt gcatatagtt accattgcaa
    58021 ttcgtttcat gatttccagc taatacatga acattttcat tgatactcat tatgcgtttt
    58081 aacaaatcga tgataaattg atcgccacag atattactaa atcgatcgcc ggtttggtcg
    58141 ccaagaaacg tacatggcgt gactgcgtct ttattaataa ccaaataagg ctgaagactt
    58201 tctaaaacat tatctcttct gttcagtata ctgattattg tattaacatt agtgtcaagt
    58261 tgctcattaa agttctccct tagcaatacg ttgagaactt catcttctat attaagcaac
    58321 tggctaagga aattttctcc ttcagccatg attcccaaat gtcctgttgc aaaaagcgcg
    58381 tacaaaactg caccgactga gccatcggta tcgccaaaat aggtcggcct ggtacttgtg
    58441 ggatcaacag gtgaacaatt ggagaaatag gttctgtcag cgaactgggg ttttataatt
    58501 gtttcgaatt taatatcaaa tacattgtat tgcgatattt cccagttcca gatgggtttg
    58561 tacgactcag taactgcaac atcattagca ttatcattta ctgtcatatt attaagtaaa
    58621 taaacaatgc tgcaccaatt aatatcggta ttatcaagag ggcaaccgta atttttacta
    58681 ttaattgtca caataaactg ataacagtta ttcctacgta gaatatgagc gcgcatgccc
    58741 gatagcatac tatatttttc tgaatgattc gtttgtatgg taaatgaaat tttgtcatct
    58801 tgaatagatt gttggaaacg ttctatttgc caatcatcaa ttttttccac aacttcaccg
    58861 agcattgtcc tggcaatact tgatatatca tcacctatac catttgcaat atacccaaac
    58921 agttctttag tgaagttggt aacatgctct tgcaccatat catttgttgc gattgtcgtt
    58981 gtactgccat tagtgcctgc cacagttcca attttcatct tttacctcat attattcacc
    59041 gcgaggaata catagttaac attttatctt atgacataca cctcatattc gatggggaaa
    59101 taattatatt ttgcacaaat cattcagcct taaagcataa tatgaaattt cttatctcat
    59161 agcagagaaa gtccctaaag attgtagagg cgtcatcagt acatacctct acaatcgatt
    59221 aagaaaacct atgaaatact ggcaaaatta aaaagagaca ccacagcact catgaagttt
    59281 tattgaggtt gaaaacaact aagccctggg acaccttttt tatcaccacg ttcgttaggt
    59341 gctatcttcc aagaatttag ccccatggta taattatcaa ttttttgatg ctggtctacc
    59401 cctaatccat aatggccaaa tttctgttta gtttttttga gcgtctgtcc acatttgaag
    59461 atatcttcag gttttggtct gaaagcagta gtgttattaa aataattttc atatacaggt
    59521 gtgggtgcta tagcattgct tctaattaga tggagaagat gatcatccat gtgttgttta
    59581 tgttttttat tacatatatt tgctaattcc tcaggatcaa ttgggtcttt catctgatta
    59641 aatggaacct gcaaagcacc gagacaataa cattgatttt cttcatttct tattattccg
    59701 tgatgactgg ttaaaacttt agtttctgag ttataattac aaacatcgag cgttttaatt
    59761 aagttatacg tatcaccggc agagagttta taattttcta gtcgtgcagt atagttacca
    59821 ttaaaattga tttcatgatt tccagccaga acgatgacat ttttattaat acggctatct
    59881 ttattaccct ccatatttcg catagaattt agcaaagtaa ggatatattt atcaccaaaa
    59941 atagtactaa atctatcgcc tgtatgatcc ccaaggaaga tacaaggggt tatcgcatcg
    60001 tcctttaaaa aaacatgttg atgcaactca ttcaatatca tgtctctcgt atttaataaa
    60061 ttgtagagca atttattatt tttatgtatg tatgcagatg aagccatgtc ttcgtgtttt
    60121 aataattcac ataataaact ccaacccttt tccctgatgc ctatatgccc tgaaacgagc
    60181 aatgaataaa gtacagcgcc tactcttcca tctgtatcgc caaaataagc cgtgttggca
    60241 ttttgtgggt taatcacacc acattgttta taaaagtcta ttttagaaaa cacagattta
    60301 acatagctat caaacttatc cttaggaaac gtctctttag agacatccca acgccagtca
    60361 gctttaaaat taccgctaag aagagtaaag aaattttcag gtatgataga aagcccatta
    60421 actttataaa ttatgtctga tttatctccg gagaaattaa taaaataata accatcctta
    60481 cactcaatct taactgtaat gggagacgat aattttatca catggttatt gtctggtata
    60541 attgtaaaga taaacgtgtt agtagtatta tcggcaaaac tattatattc ttgaatatct
    60601 atagatggct cactattgta aaaccatatt ttctccatct gaagactatg ttcatttacc
    60661 ctccagtgac catgttgcag gaaatagatg atatgcatgc caaaatcgtt atctatttgc
    60721 gaatcattat ccagcttatc aatttcaact atctttgaac gatgtgtttt atcaaccttg
    60781 ctatggtgtt gatgattatt aactttatct gaatgcttat ctttatggtt tgaagggatg
    60841 tacttaaata tattgtcaat tgctttcata aataacccgc gatatttttg acctgttagt
    60901 aaaaaataaa tgatacatgt ctgaaagaca tatttacgac gatataaaac aataatactt
    60961 atatgttcgc tgattttaag caccgagact atccggtctt ttactaaata atggaaaaca
    61021 atccatccct ggaagacaat tgtaaaaaaa acaacagaat aagaattaat cttaggataa
    61081 atatttattt atcatggcct tttgaccgct aaaaaatggc actcggcaca gtaaaccccg
    61141 ctatagttat gctatgcatt tgtctttaat gatatctcgt cgttatatta acaggctgaa
    61201 attatcatta atgcacattt caatattagc agggatacct gaggagttta aagtgaacat
    61261 atatatcggg tagcttttca aattaatccc tttgattata ggcttaattt gcatcgcgtt
    61321 aggtggcttt gtgctggaaa gttcagggca acgtcacggg tcatgtgctg atttctctgg
    61381 cggccatatg cctggcatta ttcactaccg catttattat catttcgcaa ctcacgcgcg
    61441 gcgttaatac gttttacaat acattgttcc ccattattgg ctatgcgggg tcaattatca
    61501 ccatgatatg gggttgggca ctgttagcag gtaatgatgt gatggcagac gagtttgttg
    61561 ccggccatgt tatttttggc gttggtatga ttgccgcatg tgtatcgacg gtggcagcgt
    61621 cgtccggtca ctttctgctc attcccaaaa atgcagcggg gagcaagagc gacggaacac
    61681 cggtacaggc ttattcttca ttaatcggta attgcctcat tgccgttccc gttttactca
    61741 ccctgctcgg tttcatttgg tctattacgc tgttacatag tgccgacata actccgcatt
    61801 atgtcgcctt gggttaaccg caatctgtgc ctgtctaatt ggcctagtgg ccacaatcgt
    61861 ccatcaaaca cgtaatacgt tctcatccaa agagcactgg ctgtggtgtt attgggttat
    61921 ttttctcggc tcagtcacgg tgctgcaggg gatatacgtc ttagtcagtt ccgatgcaag
    61981 cgcccgactg gctcccggca ttattctcat ttgcctcgga atgatctgtt acagcatatt
    62041 ctcaaaagtc tggctactgg cactggtctg gagacgtacc tgttcgttag ccaacagaat
    62101 accgatgata ccggtattta cttgtctgtt ttgcttgttt ttgtcatcat tcctcgcgga
    62161 aatcgcacaa actgagctgg actactttat tccatctcgg gttctggttg gattaggcgc
    62221 agtatgtttt acattgttct ctattgtttc aatattagag gccggttctg cgaaaaaaaa
    62281 ttaaaattta cataatccca gcgttattca tatcttaacg ctggtttttt acgaacttag
    62341 tgaaataatg tagaatataa aattataatt ttatattgga gcacatgtgt taatgatcaa
    62401 ccccgggata ctaaaaatat agacattatt aaacttgctt agcctcgcat tgcctttacc
    62461 tgttaaagat gcatcgagaa aattgagtct gaagttctca tcctttatct gattttccag
    62521 tcccgaaact ttggccttag cattacctga gatccgtccg ttactatcta ttttgtaggt
    62581 aaaaaaatac gaatgggaaa atttcctcgg tgtatcttca tcagttaagc caacaatcgt
    62641 aaaactaccg ctaccatctt tttttaaaat gactgaaagg ttagcagagt agtataaaga
    62701 atgatctgtt ttagcatttc ttgttaacgt cgacgtacat tcaagagacg ttgtgctacc
    62761 atcacgagcc ttccagagag caaaaaaaca cacctgtagc aatgtgaaaa caagtattaa
    62821 tataaaatac attttcttat tagtcatata atagcacctc tgaacaaatt gcataagttt
    62881 cgtcattaga ggattttata catcttgata aataccgctg cccttcccca agcaaataat
    62941 gcatactatt tacatcatat ataaaatttg cacctggcac acagggtaaa tgctttttcg
    63001 aaatttcagc ggcctctatt tgagatttag cgaggccaat ctcgttaaga ttctctgaaa
    63061 gatataaatc acaggtgtca atcttacctg atttaaccaa tggataatcc gttatactgt
    63121 aggtatcttt aaaagccacg gcaaacattt caataataac aattactacc acaagcgaaa
    63181 tcaaaatgcg aaaaagagga atactccttt tgcgctctac tttttcaaca acatcttctt
    63241 gtaaatgaga
//
LOCUS       DAJVPP010000009        57456 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVPP010000009 DAJVPP010000000
VERSION     DAJVPP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.009"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..340
                     /gene="yeiP"
                     /locus_tag="OQM34_000581"
     CDS             <1..340
                     /gene="yeiP"
                     /locus_tag="OQM34_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1451151.1"
                     /translation="GNEYVFMDKEDYTPYTFTKDQIEEELLFMPEGGMPDMQVLTWDG
                     QLLALELPQTVDLEIVETAPGIKGASASARNKPATLSTGLVIQVPEYLSPGEKIRIHI
                     EERRYMGRAD"
     gene            563..2029
                     /locus_tag="OQM34_000582"
     CDS             563..2029
                     /locus_tag="OQM34_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1451152.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2147..3133
                     /gene="yeiR"
                     /locus_tag="OQM34_000583"
     CDS             2147..3133
                     /gene="yeiR"
                     /locus_tag="OQM34_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1451153.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3172..3885
                     /gene="lpxT"
                     /locus_tag="OQM34_000584"
     CDS             3172..3885
                     /gene="lpxT"
                     /locus_tag="OQM34_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1451154.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4297..4863
                     /gene="mepS"
                     /locus_tag="OQM34_000585"
     CDS             4297..4863
                     /gene="mepS"
                     /locus_tag="OQM34_000585"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1451155.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5044..6600
                     /locus_tag="OQM34_000586"
     CDS             5044..6600
                     /locus_tag="OQM34_000586"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1451156.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            6682..8496
                     /locus_tag="OQM34_000587"
     CDS             6682..8496
                     /locus_tag="OQM34_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1451157.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGHITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HILQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            8497..9591
                     /locus_tag="OQM34_000588"
     CDS             8497..9591
                     /locus_tag="OQM34_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1451158.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKISDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            9591..10616
                     /locus_tag="OQM34_000589"
     CDS             9591..10616
                     /locus_tag="OQM34_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1451159.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            10618..12207
                     /gene="yejF"
                     /locus_tag="OQM34_000590"
     CDS             10618..12207
                     /gene="yejF"
                     /locus_tag="OQM34_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1451160.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGHCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHIVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12211..12555)
                     /locus_tag="OQM34_000591"
     CDS             complement(12211..12555)
                     /locus_tag="OQM34_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451161.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(12888..14078)
                     /gene="bcr"
                     /locus_tag="OQM34_000592"
     CDS             complement(12888..14078)
                     /gene="bcr"
                     /locus_tag="OQM34_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCS1451162.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14106..14801)
                     /gene="rsuA"
                     /locus_tag="OQM34_000593"
     CDS             complement(14106..14801)
                     /gene="rsuA"
                     /locus_tag="OQM34_000593"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1451163.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            14951..16711
                     /locus_tag="OQM34_000594"
     CDS             14951..16711
                     /locus_tag="OQM34_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1451164.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCHE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            16836..17120
                     /gene="rplY"
                     /locus_tag="OQM34_000595"
     CDS             16836..17120
                     /gene="rplY"
                     /locus_tag="OQM34_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1451165.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17259..18266)
                     /gene="yejK"
                     /locus_tag="OQM34_000596"
     CDS             complement(17259..18266)
                     /gene="yejK"
                     /locus_tag="OQM34_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1451166.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18448..18675
                     /locus_tag="OQM34_000597"
     CDS             18448..18675
                     /locus_tag="OQM34_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1451167.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            18695..20455
                     /gene="pbgA"
                     /locus_tag="OQM34_000598"
     CDS             18695..20455
                     /gene="pbgA"
                     /locus_tag="OQM34_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1451168.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            20530..20606
                     /locus_tag="OQM34_000599"
     tRNA            20530..20606
                     /locus_tag="OQM34_000599"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20564..20566,aa:Pro,seq:ggg)
     gene            complement(20709..23300)
                     /gene="yejO"
                     /locus_tag="OQM34_000600"
     CDS             complement(20709..23300)
                     /gene="yejO"
                     /locus_tag="OQM34_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCS1451169.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            23620..24267
                     /gene="narP"
                     /locus_tag="OQM34_000601"
     CDS             23620..24267
                     /gene="narP"
                     /locus_tag="OQM34_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1451170.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(24478..25530)
                     /gene="ccmH"
                     /locus_tag="OQM34_000602"
     CDS             complement(24478..25530)
                     /gene="ccmH"
                     /locus_tag="OQM34_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1451171.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(25527..26084)
                     /gene="dsbE"
                     /locus_tag="OQM34_000603"
     CDS             complement(25527..26084)
                     /gene="dsbE"
                     /locus_tag="OQM34_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1451172.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26081..28024)
                     /gene="ccmF"
                     /locus_tag="OQM34_000604"
     CDS             complement(26081..28024)
                     /gene="ccmF"
                     /locus_tag="OQM34_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1451173.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28021..28500)
                     /gene="ccmE"
                     /locus_tag="OQM34_000605"
     CDS             complement(28021..28500)
                     /gene="ccmE"
                     /locus_tag="OQM34_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1451174.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(28497..28706)
                     /gene="ccmD"
                     /locus_tag="OQM34_000606"
     CDS             complement(28497..28706)
                     /gene="ccmD"
                     /locus_tag="OQM34_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1451175.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(28703..29440)
                     /gene="ccmC"
                     /locus_tag="OQM34_000607"
     CDS             complement(28703..29440)
                     /gene="ccmC"
                     /locus_tag="OQM34_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1451176.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(29482..30144)
                     /gene="ccmB"
                     /locus_tag="OQM34_000608"
     CDS             complement(29482..30144)
                     /gene="ccmB"
                     /locus_tag="OQM34_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1451177.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30141..30758)
                     /gene="ccmA"
                     /locus_tag="OQM34_000609"
     CDS             complement(30141..30758)
                     /gene="ccmA"
                     /locus_tag="OQM34_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1451178.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(30777..31379)
                     /gene="napC"
                     /locus_tag="OQM34_000610"
     CDS             complement(30777..31379)
                     /gene="napC"
                     /locus_tag="OQM34_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1451179.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31389..31838)
                     /gene="napB"
                     /locus_tag="OQM34_000611"
     CDS             complement(31389..31838)
                     /gene="napB"
                     /locus_tag="OQM34_000611"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1451180.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(31835..32698)
                     /gene="napH"
                     /locus_tag="OQM34_000612"
     CDS             complement(31835..32698)
                     /gene="napH"
                     /locus_tag="OQM34_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1451181.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(32685..33380)
                     /gene="napG"
                     /locus_tag="OQM34_000613"
     CDS             complement(32685..33380)
                     /gene="napG"
                     /locus_tag="OQM34_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1451182.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33387..35873)
                     /gene="napA"
                     /locus_tag="OQM34_000614"
     CDS             complement(33387..35873)
                     /gene="napA"
                     /locus_tag="OQM34_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1451183.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(35870..36133)
                     /gene="napD"
                     /locus_tag="OQM34_000615"
     CDS             complement(35870..36133)
                     /gene="napD"
                     /locus_tag="OQM34_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1451184.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36123..36617)
                     /gene="napF"
                     /locus_tag="OQM34_000616"
     CDS             complement(36123..36617)
                     /gene="napF"
                     /locus_tag="OQM34_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1451185.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37025..37513
                     /gene="eco"
                     /locus_tag="OQM34_000617"
     CDS             37025..37513
                     /gene="eco"
                     /locus_tag="OQM34_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1451186.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(37663..38694)
                     /locus_tag="OQM34_000618"
     CDS             complement(37663..38694)
                     /locus_tag="OQM34_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCS1451187.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(38928..39287)
                     /locus_tag="OQM34_000619"
                     /pseudo
     CDS             complement(38928..39287)
                     /locus_tag="OQM34_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(39505..41148)
                     /gene="yojI"
                     /locus_tag="OQM34_000620"
     CDS             complement(39505..41148)
                     /gene="yojI"
                     /locus_tag="OQM34_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1451188.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41224..41874)
                     /gene="alkB"
                     /locus_tag="OQM34_000621"
     CDS             complement(41224..41874)
                     /gene="alkB"
                     /locus_tag="OQM34_000621"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS1451189.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(41874..42938)
                     /gene="ada"
                     /locus_tag="OQM34_000622"
     CDS             complement(41874..42938)
                     /gene="ada"
                     /locus_tag="OQM34_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1451190.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43012..44067)
                     /gene="apbE"
                     /locus_tag="OQM34_000623"
     CDS             complement(43012..44067)
                     /gene="apbE"
                     /locus_tag="OQM34_000623"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1451191.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44179..45300)
                     /gene="ompC"
                     /locus_tag="OQM34_000624"
     CDS             complement(44179..45300)
                     /gene="ompC"
                     /locus_tag="OQM34_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1451192.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46038..48710
                     /gene="rcsD"
                     /locus_tag="OQM34_000625"
     CDS             46038..48710
                     /gene="rcsD"
                     /locus_tag="OQM34_000625"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1451193.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTTVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            48727..49377
                     /gene="rcsB"
                     /locus_tag="OQM34_000626"
     CDS             48727..49377
                     /gene="rcsB"
                     /locus_tag="OQM34_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1451194.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(49577..52426)
                     /gene="rcsC"
                     /locus_tag="OQM34_000627"
     CDS             complement(49577..52426)
                     /gene="rcsC"
                     /locus_tag="OQM34_000627"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1451195.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(52701..53477)
                     /locus_tag="OQM34_000628"
     CDS             complement(52701..53477)
                     /locus_tag="OQM34_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCS1451196.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53482..55131)
                     /locus_tag="OQM34_000629"
     CDS             complement(53482..55131)
                     /locus_tag="OQM34_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1451197.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55132..55691)
                     /locus_tag="OQM34_000630"
                     /pseudo
     CDS             complement(55132..55691)
                     /locus_tag="OQM34_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(55850..57409)
                     /locus_tag="OQM34_000631"
     CDS             complement(55850..57409)
                     /locus_tag="OQM34_000631"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451198.1"
                     /translation="MTTSELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFY
                     PLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPS
                     LTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVY
                     RVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLS
                     AELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETP
                     QEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRL
                     MQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQA
                     DNMPLLHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILD
                     TPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGG
                     DATQTAAILMV"
BASE COUNT        14055 a        15515 c        14105 g        13781 t
ORIGIN      
        1 tggcaacgaa tatgtcttta tggataaaga agactatacc ccgtatacct tcaccaaaga
       61 tcagattgaa gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct
      121 gacctgggac ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt
      181 tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt
      241 gagcactggt ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat
      301 ccatatcgaa gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa
      361 gggatagctc aggctgtccc ttttttaatt tattatacca atcttctatt ttgcgcttcc
      421 tgaacacctt acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat
      481 taacaatcca gaccaatttc aattgctgtc atataacttt acactgtcat tgttaattta
      541 tcgttactaa gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt
      601 acacgctcca cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg
      661 cgcctttcac cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg
      721 cgactggggg atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg
      781 cgcacagaac catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat
      841 tgtcggtgcc gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga
      901 gaaattttgc gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg
      961 tattgacccg gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca
     1021 aacccctgaa gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg
     1081 tgagcgcagc cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca
     1141 tgtggtgaaa aacgcggtgc tgagaatggc agaaaaacgt tcgccagaac tcgccgggtg
     1201 gataaaagaa cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgcaac
     1261 cgacgaatca ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag
     1321 ctgcgaaccg tttatccagt gggtggttga agataacttc atcgctgggc gtcctgcctg
     1381 ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg
     1441 tatgcttaat ggcagccact cttttctcgc ttatctgggt tacctctcag gattcgccta
     1501 tatcagtgat tgcatgcagg atcgcgtatt tcgctatgct gccagaacat taatgctgga
     1561 tgagcaagcg ccgacactgc aaattaaaga tgtcgattta acacaatatg cggataagtt
     1621 aattgcacgt tttgctaatc cgtcgctgaa acataagacc tggcaaatcg cgatggatgg
     1681 cagccagaaa ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgtgaaac
     1741 ggactggtcg ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga
     1801 tgatgccgga aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt
     1861 tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg
     1921 tgatgatctg ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat
     1981 cgtacaattc ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg
     2041 gttaaagcgg atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag
     2101 ccaggattaa cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa
     2161 cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca
     2221 taaagatccc aacgaaaaat gggcggtact ggttaatgaa tttggggaag tcggaattga
     2281 tggtgctttg ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg
     2341 ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc
     2401 agaccgcttg ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt
     2461 aaccgcacca gtctatgaac cgtggataga tctgcgcgcc accttgtgca ttctcgatcc
     2521 acgcctgctg ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc
     2581 cgcagacatc attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct
     2641 acagcgttgg tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa
     2701 agttgacggt catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc
     2761 cgcgcattct catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca
     2821 tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt
     2881 cgacgctgat acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt
     2941 ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg
     3001 cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat
     3061 ttccagcagc gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc ttcgtttagc
     3121 gactaccgcg taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa
     3181 aatttgccgc aaatagtgtt gttgaatatt gtcggccccg cgctgtttct ttcctggtat
     3241 attcccgtta atcatggatt ctggttgccg attgatgcgg atatttttta tttctttaat
     3301 cagaaactgg tcgaaagtaa ggccttttta tggctggttg cattgactaa caatcgcgcc
     3361 ttcgacggtt gttcactgct ggcgatgggt atgttgatgc tgagtttctg gctgaaagaa
     3421 aacgcccctg gcagacgacg tatcgtgatt atgggtctgg tcatgctatt aactgcagtg
     3481 gtattaaacc agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgact
     3541 tttaccgata ttaaccgcgt cagcgaactg ctctctgttc ccacgaaaga tgcctcacga
     3601 gatagctttc ccggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt
     3661 tatttcggca aagttgcagg ccttatcgcc cttattattt ttgtggtttt tgcatttccc
     3721 agagtaatga ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata
     3781 ttgatcggtt tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt
     3841 gacaaatcac taccaggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac
     3901 atcatcaggg ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatct
     3961 atcgaacgca aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaattg
     4021 tactttaatt gaccaacccc gcttattaac tttctgtatc actttttctt ataaaaaatc
     4081 atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt tttgcctcgg
     4141 cggtagatta tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg
     4201 aattgtttca acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat
     4261 atttgtcgtt aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg
     4321 agatatatct tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc tgcatgtagt
     4381 gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca
     4441 tcactgcaag cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg
     4501 cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact
     4561 aaaaaaggta tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta
     4621 gaacttccgc gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat
     4681 ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt
     4741 tatatcggca acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc
     4801 atgaatgaac cgtactggaa gaagcgttac aacgaagcac gccgggttct cagccgcagc
     4861 taataaaccg tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct
     4921 taggcagtgc ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca
     4981 ggctatagtc cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga
     5041 atcatgttca tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca
     5101 ggcgtgatga ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac
     5161 gaagtcaaat acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc
     5221 gacctgaaat ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct
     5281 aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg
     5341 aaagataaaa aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa
     5401 ttgattccgg cgctcgacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg
     5461 atggtgccga acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc
     5521 ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag
     5581 gaagattacg atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca
     5641 cgtttgatga acgttaatga attaaccgac atgccggtcc gtgaaactaa aattgcgggc
     5701 attcctctga ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca
     5761 tttttactgg gcggcatgag tggaactgtc gttggcctgc tttgctatta cctgatgagc
     5821 gtacgtatgc gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg
     5881 gcgtatcaac cggtggtgga tacacaagct ttgcgggtaa cgggcctgga agtactgcta
     5941 cgctggcggc atcctgtagc gggagaaatt cccccggatg ccttcattaa ctttgccgaa
     6001 tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc
     6061 gcagaattag aaaaagtact gccggtaggc gtcaaatttg gcattaacat tgcgccggac
     6121 catctgcaca gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca
     6181 caccatttcc agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc
     6241 acacaactct tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc
     6301 accgggcaca gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac
     6361 cgtggattta tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg
     6421 ctgacgctgg cgaaacgtct caatatgctg acagttactg aaggggtcga aacgccggaa
     6481 caagcgcgat ggctaagcga acgcggcgtt aatttcatgc aaggctactg gattagtcac
     6541 ccgttaccgc tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc gcagtggtaa
     6601 ggtgtgctta cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga
     6661 gtaaaaggaa atccgttgca gatgattgtg cgcatactgc tgctgtttat cgctctgttc
     6721 accttcggtg cgcaggcgca ggctatcaag gaaagctata cctttgccgt gctgggcgaa
     6781 ccccggtacg cgtttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt
     6841 gggcatataa cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc
     6901 ggcaacccgg gcgcacgcac cgagcagctg tacgacacgt tatttacgac ttccgatgac
     6961 gaaccaggca gttattaccc gctgattgct gaaagtgcac gctatgctga cgattattcc
     7021 tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg atggttctcc cattactgcc
     7081 cgcgatgtag agtttacttt tcaaaaattt ctgaccgaag gcgtgccgca atttcgtctg
     7141 gtctacaaag gcaccaccgt caaagccatt gcgccgttaa ccgtgcgcat tgagttagct
     7201 aaacccggca aagaagatat gttgagtctg ttttcgctgc cggtatttcc agaaaagtac
     7261 tggaaggatc acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac
     7321 cacgttacgt cctggaaaat ggggcaaaat attgtctatt cccgcgtgaa agattactgg
     7381 gcagcaaact taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat
     7441 tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg
     7501 gaaaacgacg ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac
     7561 atcatcaaag atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat
     7621 atccaacgtg cggtattcag cgatcgccgg gtgcgggaag caatcactct cgcctttgac
     7681 tttgaatgga tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt
     7741 caaaataccg aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca
     7801 ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct
     7861 aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg acaaacttct caacgaagcg
     7921 ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa cgggtcagcc gctcagcttt
     7981 gaattattgc ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacatcctg
     8041 caacggctgg gtatcaatat ggacattcgc aaggtggata actcgcaaat cactaaccgc
     8101 atgcgcaatc gcgactatga catgatgccg cgcctatggc gggcgatgcc gtggcccagt
     8161 tccgatttac agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc
     8221 gtgcaaagcc cggttatcga ctcactgatc aaccaaatta ttgccgcgca gggaaataaa
     8281 gaaaaattac tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg
     8341 ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccaaccc
     8401 gctgtacgcc ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg
     8461 gccaaactgc cgtcagccag acaacaggga gagtagatgg gcgcttacct gattcgccgt
     8521 ctgttgctgg tgatcccaac gttatgggct attatcacta tcaacttttt catcgtgcaa
     8581 attgcgcctg gcggtccggt cgatcaggcc atcgccgcca ttgagtttgg taatgccgga
     8641 gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc
     8701 aatatcagcg acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag
     8761 atcactcatc gctacggttt cgataagccg atccacgaac gttacttcaa aatgctctgg
     8821 gactacatcc gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg
     8881 attaaagaca gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat
     8941 ctggtgtcga ttccgttagg cattcgcaaa gctgtttata atgggagtcg ctttgacgtc
     9001 tggagtagcg catttatcat catcggctac gccattccgg cctttttgtt tgccatcctg
     9061 ctgattgtct tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt
     9121 tccgctaact ttgattcgct gccgtggtat cagaaaatca gcgattatct gtggcatatc
     9181 acgctaccgg tgctggcgac agtgattggt ggctttgcgg cgctgaccat gctgaccaaa
     9241 aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta
     9301 agtgagaaaa acattctctg gaaacatatg ttccgcaacg ccatgctgct ggtgattgcc
     9361 ggttttccgg cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg
     9421 ttttcactca atggtcttgg cttactgggc tacgaagcga ccgtctcgcg cgattatcct
     9481 gtaatgtttg gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt
     9541 gatatcagct atacgctggt tgatccgcgt atagattttg agggacgtta atgtcgcgac
     9601 tcagccccgt caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt
     9661 cgttatggat tttcctcgtt ttgtttggtt tgagtttgtg ttctgaactt atcgctaacg
     9721 ataaaccgtt gctggtgcgt tatgacggca gttggtattt cccgttgttg aaaaactaca
     9781 gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga
     9841 aacaacggct ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca
     9901 gtatcaactt tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg
     9961 gaacggatgc aaacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg
    10021 ttctgtttgg cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc
    10081 tacaaggcta ttacggcggt aaagtcgatc tctgggggca acgctttatt gaagtatggt
    10141 cggggatgcc gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct
    10201 ggtggctgct ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg
    10261 cggagttttt acgtactcgt aatttcgact acattcgtgc ggcacaggcg cttggcgtca
    10321 gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct
    10381 ttttaccgtt tattttatgt agttcgataa ccaccctgac ctcgctcgat ttcctcggct
    10441 tcggtctacc gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc
    10501 ttcaggcccc gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc
    10561 tgatctttat tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg
    10621 acgcaaactc tgttagcgat tgaaaatttg tcggtgggtt ttcgccatca gcaaaccgta
    10681 cgtacagtag tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg
    10741 ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc
    10801 ccgccggttg aatatctctc cggcgatatt cgttttcatg gcgaatcgct gcttcatgcc
    10861 agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaggaaccg
    10921 atggtgtcgt taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc
    10981 caccgcggga tgcgtcggga agcggctcgt ggcgaaattc ttaactgcct tgatcgcgtc
    11041 ggtatccgcc aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa
    11101 cgccagcggg tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat
    11161 gaaccgacca ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa
    11221 ctgcaaggcg agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga
    11281 aaactggccc accgcgtggc ggtaatgcaa aacggtcact gtgtcgagca aaataacgcc
    11341 gctacgctat ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg
    11401 tcaggcgatc cggtaccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt
    11461 caggttgcct tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg
    11521 gtgaaaaaca tcagttttac gctacgggcg ggtgaaacac tgggtttagt gggcgagtct
    11581 ggttccggga aaagcacgac gggactggcg ctgctgcgac tgattacctc tcagggcagc
    11641 atcgtctttg acggtcagcc actgcaagat ttaaatcgcc gccagctgtt acctactcgt
    11701 catcgtattc aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt
    11761 ttgcagatta ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc
    11821 gaacaacaag tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt
    11881 tatccggcgg agttctctgg tggtcagcga caacgtattg cgattgccag ggcattaatt
    11941 cttaagccct cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag
    12001 gcgcaaatat tgacgctttt gaaatcattg caacaaaagc atcaactggc ctatttgttt
    12061 atcagccacg atttgcacat tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa
    12121 ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg ccacaccgca gcaggagtat
    12181 acgcgtcagt tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc
    12241 ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt
    12301 tacaaacagg cacggctcac cttcacactc caacaccgaa catggaactt cgatgtcgct
    12361 taacgcctgg cttagtttat acatcaccga ccatgcatta tcaccatccg ggctaagcaa
    12421 tttaagcgcc accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt
    12481 cgaacctgcg aaaccagcag accagcgata gttctgcggc agtcgatgga cgattgagag
    12541 ttgtaatgaa gtcacttatt ttccccggaa gtacattcac ttcacaattt gtttacatca
    12601 attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct
    12661 gcgtggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc
    12721 tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt
    12781 acataaattg attttacata aaataaacat atatcgggga aatgatagat ttgtggttga
    12841 catgcatcaa caaaggaagc cttttacctt cctcgttgtg caatagatca ccgttttttc
    12901 ggccgactgg cgtacagaca gaagagaatg gagctggttg cgcagaatgc aattgaccaa
    12961 atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct
    13021 atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca
    13081 tcaagaatga ccgccatcgc attggatgac accatcgaca cgcagcccac aaacgccgca
    13141 acgccaacca ccagcgacca gaatcccagc cccagcggcg cactgatgac catccacgca
    13201 gccataataa attgtatcca caaccccgag cggaacatat ttaacgcgcc aatgcggcgg
    13261 acgaagcggc tgttaaagat ggtcatcacg aacagaaaaa caatgtttag cgcaaagtaa
    13321 taaccaaagt tttccggcgc gacgtggtta atttcaatat aaacaaacgg tccggcgctt
    13381 aagaatgaga acatcccggc aaagctgaaa ccactggcaa gcatgtagct caggacacgt
    13441 ttatggcgga acagcgccgc aaagttacca atagtggtac gaatgtgaaa tggctgacga
    13501 cgctccggtg gtaaggtttc tttaatcagg aagaaaatca ttgccgaagc cagaatcgcc
    13561 gctaatgcca ggatccagaa gatgtaatgc cagctcagcc acaccagcac ccagccgcca
    13621 actatcggtg ccatcagcag tgcaatggtc gtcaccagca tgacaaacga catcatccgc
    13681 gagaactctt ctttcgggta aatatcacgc atcaaagcgt tgataaccac gctggccgca
    13741 gccgcagcca gcccgtggaa gaaacgcatc acaatcagct gatcgatggt ttgtgccaac
    13801 gcacacgcca ccgcggcggc ggcaaacacc agcgtcccgc caagaaccac cggcttacgc
    13861 ccgaagctgt ctgccatcgg cccgtagatt aactgcccca acgcaaagcc cagaatataa
    13921 gtactgaggg tcatctgcgt actgcccgcc gatacgccaa actgcgctga aattaccggt
    13981 agcgcgggca gatacatatc aatcgacagc ggcatcaaca tggccagcag gccaaggata
    14041 aaaacaatag caaacgacga atgctgtcgg gtggtcacaa cgggctcctg aaagtcattg
    14101 aaaagttaga cgacgctggc aatttcttct tcagttaacg gacgatattc accgggggct
    14161 aaatcagcat ccagcgtaat accgccaata cgttcacgat gcagctcaac cacgtggtta
    14221 cccacggcgg cgaacatgcg tttcacctga tgataacgcc cttcgctgat ggtcagacga
    14281 acctgcgttg gggtaatcac ttcaagcacc gccggcttag tgagatcttt ttcgttatgc
    14341 agctgcacgc ctttagcaaa ttgctctgcc gtatcgtcag ctacaggtga ttccagtgtc
    14401 accagatagg tcttctcgca atgatggcgc ggagaagtaa tgcggtgcga ccactgacca
    14461 tcatcagtca tcagcaccag accggtggta tcgatatcca gacgtcctgc ggcgtgcagt
    14521 ttccacgcta ccggttcatc cagaaaatag agcaccgtcg ggtgatccgg gtcgtccgtg
    14581 gagcaaacat agccctgagg cttattgagc atgaagtaac gtggaccgtg ttgctgcgcc
    14641 agcgggttgc catcgtaagc gacatcatgt tcagggagca gtttgaacgc tgcattacga
    14701 acgatttcgc catcgacggt gacacgattg ccgcggattt cacgcccggc aatagcacgg
    14761 ctaacgccga gttgctgtgc gataaattta tcaagtcgca tgtgtgtgat tttgcctgta
    14821 aaaaacggag gtcgggtaat gacccgaaaa tctgaactgt tgtctgccca gtatagcggt
    14881 ctaataactt ccctcaaggg aaaaagaacc gtggcatact attagcagaa taatctacct
    14941 acgcgaaacc atgattttta cactccgccc atatcagcaa gaagccgtgg atgccacgct
    15001 caaccatttt cgtcgtcata aaacccctgc cgttatcgtg ctgcccaccg gcgcaggtaa
    15061 aagcctggtg atagcggaac tggcacggct ggcgcgtggt cgcgtactgg tgctggcaca
    15121 cgttaaagaa ctggtggcgc aaaaccatgc aaagtatcag gcgctggggc tggaagccga
    15181 tatttttgcc gccgggctaa agcgcaaaga gagccacggt aaagtggtat ttggcagcgt
    15241 tcagtcggtc gcccgtaatc ttgatgcctt tcagggtgaa ttttcgctgt tgattgtcga
    15301 tgaatgccac cgtattggtg acgatgaaga gagccagtat cagcaaatcc tcactcacct
    15361 gactaaagtg aatccccact tacgcctgct ggggctgact gccacgcctt ttcgattggg
    15421 caaaggctgg atctaccagt tccattatca cggcatggta cgcggcgatg agaaagccct
    15481 tttccgtgac tgcatttatg agctgccgct gcgttatatg attaaacacg gctatctgac
    15541 gccgccagaa cgactggata tgccagtagt gcaatacgat ttcagccgct tgcaggcaca
    15601 gagtaacggg ctgttcagcg aagctgatct caaccgtgag ctgaaaaaac agcaacgcat
    15661 tacgccacat atcatcagcc agatcatgga gttcgccgaa aagcgcaaag gggtaatgat
    15721 tttcgccgcc accgttgaac acgcaaaaga gattgtggga ttactgcctg ccgaagatgc
    15781 tgcactgatt actggcgaca accccggcgc tgagcgcgat gtgttaattg aagattttaa
    15841 agcccagcgt tttcgctatc tggtcaacgt cgcagtactg accaccggat ttgacgcccc
    15901 gcacgtcgat cttatcgcca ttctgcgccc taccgaatca gtgagtcttt accaacaaat
    15961 tgtcgggcgc ggtctgcgtc tcgctccggg caagactgat tgtttaattc ttgattatgc
    16021 gggtaatcct cacgatctct acgcgccgga agttggtaca ccgaaaggca aaagtgacaa
    16081 cgttccggta caggttttct gccctgcctg cggttttgcc aacacctttt gggggaaaac
    16141 gaccgccgac gggacattga ttgaacactt tggtcgccgc tgtcagggat ggtttgaaga
    16201 tgacgacggt catcgcgaac agtgtgactt ccgtttccgt tttaaaaatt gcccgcaatg
    16261 taacgcagaa aatgatattg ccgctcgccg ctgccacgaa tgtgacacgg tactggttga
    16321 tccggatgat atgttaaaag cggcgctacg actgaaagat gcgctggtat tacgctgtag
    16381 cggcatgtct ttgcagcatg ggcacgacga gaaaggcgaa tggttgaaaa tcacctatta
    16441 cgatgaagac ggcgcggatg tgagtgagcg tttccgtctg caaacgcccg cccagcgtac
    16501 cgccttcgag cagcttttta tccgcccgca tacgcgcaca ccgggcatcc ctctgcgctg
    16561 gatcaccgcc gccgatatcc tcgcccagca agccttactg cgacatccgg attttgtcgt
    16621 cgcccgcatg aaaggtcagt actggcaagt gcgtgaaaaa gtgttcgatt acgaaggtcg
    16681 ttttcgtcgg gcgcacgaat tacgcggtta atggtagttt tgattgatgt ataaggcgat
    16741 tgagtataga atctcgcccg cttttgcata cgcaaagcag atcacttacc tgttgctggg
    16801 tcgcctgtag caggattaat ttaagagaga aagaaatgtt tactatcaac gcagaagtac
    16861 gtaaagagca gggtaagggt gcgagccgcc gcctgcgtgc cgctaacaag ttcccggcaa
    16921 tcatctacgg tggcaaagaa gcaccgctgg ctatcgagct ggatcacgac aaagtcatga
    16981 acatgcaagc taaagctgaa ttctacagcg aagttctaac catcgttgtt gacggtaaag
    17041 aaatcaaagt taaagctcag gacgtacagc gtcacccgta caaaccgaag ctgcagcaca
    17101 tcgacttcgt tcgcgcttaa ttgctgaata agttgtaaaa aaccccgctc cggcggggtt
    17161 ttttgtatct gcagattatg cctgattacg gtattgctat tttttgcagg ccagataagg
    17221 cgttcacgcc gcatctggca ttcagaacac aaaaccgatt aattgccgcc agacgtccgg
    17281 cgctgcaatt ggtcgcgcaa attcggcggc gtgcctttaa tggtcaaggt atcggttgct
    17341 ggatcccaga agatgcgttc acctaacagc atcgcatcaa agttaatagt caagccaccg
    17401 ccgctaccgg caaacttcgt caactgacgc agtgtgctac ggtctgccgg gaagctctct
    17461 tccagttcgt agcctttttc agccgcaaac tccgtgaagc tcacttcgct tacgcctgcc
    17521 agctctttcg acagcgattc cagttcgatc tcttcgcctg cctgcagctg ctcattacag
    17581 tagctataaa cctgctggcg cacgttctgt cgctctgctt tatccagttg tgcttcggca
    17641 gtgaaatcat caaccgcttg caacagccca cggttttgcg ctttcgcgtt caatccttcg
    17701 ctggccccga ggaaatccat aaagaaatcc gccactttgc gccctactcg ccctttcagg
    17761 aaagtgagat agcgggtgga ctctggattg gtttcccatt cggttaaatc gatacgcgca
    17821 acaatatccg catgattgat atcaagataa tgggttgggt tgatgtcgag attttcgtta
    17881 acgcgcatac tgctcaggtt gctcagaact gccaccagca aatactccac cgccagataa
    17941 cggtagtggc aaaacagcac gaatccaccg tcagcaaaag gatatttcgc taattcgtca
    18001 cgcaggcgac cggttgccgc gcggctaaat gccagaaagt cctcttcgcc ctgacgctgt
    18061 aagcgcagcg tctgcgccag ttcactctct tcgctaaaca agccgtaagc tttatttttg
    18121 gcgctataga cccgatgcag ttctgccacc atctcaacga cggtttctgt cggttccagc
    18181 aatgaatcgc gcaacaccag ctcaaggttc tgctcatcac gcttgataag ctggtgcaga
    18241 gcaatctggt tgatatccag actcatgata aactctcctt taagaccggg cggtattcaa
    18301 ccaccgccag ttaagcgacg caataaaaga tggcggtcat tttacagaag gcagcgtaac
    18361 gagaatgatt taacgcataa aaaagcagaa aaaaaaccgt tgctacggta atatgttgcc
    18421 ctttcatcaa ctaactgatt tcgatttatg ccacaaattt cccgctacag taatgaacag
    18481 gttgaacaat tgctcgcaga gctgctcaac gtactggaaa aacataaggc tccgaccgac
    18541 ctttccctga tggtgttagg caacatggtc actaacctta tcaacaccag cattgccccg
    18601 gcccaacgcc aggcaattgc caactctttt gccagcgcct tacagtcctc tatcaacgaa
    18661 gacaaagcgc actaagggaa acagataaca ggttatggta actcatcgtc agcgctaccg
    18721 tgaaaaagtc tcccagatgg tcagttgggg gcactggttt gcactgttca atattctgct
    18781 ttcgctcgtc attggcagcc gttacctgtt tatcgccgac tggccgacaa cgcttgctgg
    18841 tcgcatttat tcctacgtaa gcattatcgg ccatttcagc ttcctagtgt tcgccaccta
    18901 cttgctgatc ctcttcccgc tgacctttat cgtcggctcc cagaggctga tgaggttttt
    18961 gtccgtcatt ctggcgacgg cgggaatgac gctattactg atcgatagcg aagtctttac
    19021 tcgtttccat ctccatctta atcccatcgt ctggcagttg gttatcaacc cagacgaaaa
    19081 tgagatggcg cgcgactggc agctgatgtt catcagcgtg ccggttattt tattgcttga
    19141 actggtgttt gcgacgtgga gctggcaaaa gctgcgcagc ctgacgcgtc gtcgacgctt
    19201 cgcgcgcccg ctggccgcat tcttatttat cgcctttatc gcctcgcatg tggtgtatat
    19261 ctgggccgat gccaacttct atcgcccgat caccatgcag cgcgctaacc tgccgctttc
    19321 gtacccgatg acggcgcgac gtttccttga gaagcatggc ctgcttgatg cgcaggagta
    19381 tcaacgccgt cttattgagc aaggtaatcc agacgccgtt tccgtacagt atccgttaag
    19441 cgaactgcgc tatcgcgata tgggcaccgg gcagaatgtg ctgttgatta ctgtcgatgg
    19501 cctgaactac tcacgcttcg agaagcagat gcctgcgctg gcaggttttg ctgagcaaaa
    19561 tatttcgttc acgcgccata tgagctccgg caacactaca gacaacggca tctttggcct
    19621 gttctatggc atctcgccga gctatatgga cggcattctg tcgacccgta cgcctgcggc
    19681 attaattact gcgcttaatc agcaaggcta tcagctgggg ttattctcat cagatggctt
    19741 taccagcccg ctgtatcgcc aggcattgtt gtcagatttc tcgatgccga gcgtacgcac
    19801 ccaatctgac gatcagaccg ccacgcagtg gatcaactgg ctgggccgct acgcacaaga
    19861 agataaccgc tggttctcgt gggtttcttt caatggcact aacattgacg acagcaatca
    19921 gcaggcattt gcacggaaat atagccgggc ggcaggcaat gtcgatgacc agatcaaacg
    19981 cgtgctcaat gcactgcgtg attctggcaa actggacaat acggtggtga ttatcactgc
    20041 cggtcggggt attccactga gcgaagagga agaaaccttt gactggtccc acggtcatct
    20101 gcaggtgcca ttagtgattc actggccagg cacgccggcg cagcgtatta atgctctgac
    20161 tgatcatacc gatctgatga cgacgctgat gcaacgcctg ctgcatgtca gcacacctgc
    20221 cagcgaatat tcgcaaggtc aggatttgtt caaccctcaa cgccgtcatt actgggttac
    20281 tgctgcggat aacgatacgc tggcaattac caccccgaaa aagacgctgg tgctgaacaa
    20341 taacggtaaa taccgcactt acaacttacg tggtgaaaga gtgaaagatg aaaaaccaca
    20401 gttaagtttg ttattgcaag tgctgacaga cgagaagcgt tttatcgcta actgattaat
    20461 tataaatcag ttagcgaaat atcttacttg caatcggtgt ggaaaacggt agtattagca
    20521 gccacgagtc ggcacgtagc gcagcctggt agcgcaccgt catggggtgt cgggggtcgg
    20581 aggttcaaat cctctcgtgc cgaccaaaaa tcccaagaaa aaaccaaccc ttacggttgg
    20641 tttttttata tctgcaatta attcgataaa cagaccgtga cacatcacag cctgtttatt
    20701 ttctgttatc agaacgtcca gaccacaccc gcctgagtat tccacggcga ttctacccct
    20761 gcgccattgc catagctgac tgacaaatga ccgcttaacg tcggggtaaa cgatgacctt
    20821 atcccagcct gataaacgcc actggttcca gccacatcat tattaaattt gccatcgtca
    20881 ttaactttca cctggttaga atcggcgtat tcctgacgca cggcggcttt cagccagggt
    20941 tccagcgtcg taccgttttg caggtccatg tgatagctta ccgccgttcc cgcttcagcg
    21001 cgtaatatcc gggtatttcc cacatccgcg cgcatgccgt ttgataacgt gtagtcctga
    21061 ccatctgtgg taaagccggt aaaggccaga tagggtctaa cactccacaa tccgtcaacc
    21121 caacggaacc cgctctcaac atgagcaccc gcgccgttac tattgtaatc gccaaacgct
    21181 gttgccccat tactcatctt gccatggatg gtgttggcaa aacggtcaac tttcaccacc
    21241 ccatcaacat aggcaccgtt ctgatgctcc caaccggcat aagcccccag ggtatagcta
    21301 tcgatattac cttttccgcc gcgatcaaaa ccaatatcag aatgagagta accaaagaac
    21361 aagccgcgaa ttgtactgct ttcttcacgg gagaaacggc tatcgatacc gagcgtcagg
    21421 cccgtcaatg tttgctcaaa accagctccc gcatcagtgg tcacgttgtt gcgggtgtta
    21481 attgccgaac tccacatcac cgtatcgtaa ctaacgcctt ttacgctacc aagacgctca
    21541 cgcacggtgt ccagttctgc atcaaatacc agcggttgtg cggctgccat gttcagcaca
    21601 tcagtggttg aaggggtaat ttgcgcgcga ttctctgcga ggctccagct atggttgcca
    21661 ttatccagca aggtatattc atacgtaccg atatcaacaa cgcctccggc attgcccaac
    21721 gtaaatgcag catcaccgcc gcccgttgtt accagtgtaa ggctatctcc tgctgccggg
    21781 ctggcaccgg tgtccgtcac gaatattttg aaatcacctg ttgcctgacc ggtgacgttg
    21841 agctgatcgc tctgatgatt ggccatatcg gtacgcatat aaaaattgcc gtttcccgac
    21901 agggtattgg tggtcaacgt aatgaaattt tccgcacttg ccgtcacaga tgaacgagtc
    21961 actggctcag ccatcataat cacatttgcg ccggacatcg caaggttggc taagttgagg
    22021 cgagtgtttt ggttcgtcgt agtgatatcg gcaatgatgg tccatgcgct attttcgagc
    22081 gaaacgtccg ctttgctggt atccacggca ccatgcgttc taaaagaagc gccctcagaa
    22141 accactacct tacccaccaa catagcgggt gcataattta tttgcggctt catgacctca
    22201 agaatgccat cattccctct gactgacacg ttaaccattg tgccagccca gacattagcg
    22261 cggccattgt tgattaccat attttcagag atggcttttg aggaatacgt gatacttcca
    22321 ttctggtctg atcgaccgag atcatatacc gcatttgcct gcactttcgt accagtatcc
    22381 tttcccagcg attgcatagt ggcatgctca tcaaggatag tgtcaatggc ctgcgtgtcc
    22441 tccataacaa tgagcccgga accatcatcc agttcataat tttttgacac accatccttt
    22501 atactaaatt ggcctttact gtttgtacca ctcacttcca gcgcattcgt tgagacaatt
    22561 aatttcccgc ctgctttttt atcaacgcca gttgcagtcc cgccatccat aacctccagt
    22621 aagccgccac tatccacagt agtattataa gcgaagtcat tttcttcaac tcgcaaactt
    22681 ccgccgtttt ccaacaacat attattagca ataccattct tgatatcgaa ctgaccgaga
    22741 cggtttgttc cgaatacctc catggcccgc gtggttgttt taatagcacc gcctgctttc
    22801 tgatctaccg caagcgcaaa tcccccctct ttaacctcca gtagtcctcc cttttcgata
    22861 gcggcaaaaa cggtcccccc atctttttca acgattaagc gcccgccttt aatcgtggta
    22921 tcattcgctg ggccataaac ctgtagcaca ccattattga gggtagtctg gtttgccgcg
    22981 ccacccattt gaacaatttg ataaccatca tttaatactg agtattctgc ggtaccattc
    23041 atttcaatgt attgataccc tccgttaatt tcagtattac tacttacacc aaattctttt
    23101 atatgctgtt ctccgccagg attgattttc gtattattgg cggtcccata aaccagctgg
    23161 atatcttttt ccaggataac accatcaact gtctcaccat actcaactgt tgatgccaat
    23221 gctatgggtg aatataacgc tgtagccacc agaacagata ttgcggaacg acaaagagaa
    23281 acagaaccag attgatgcat tgagctttca tcctatgaaa ttaattgcag ttaaaagcat
    23341 tgggtacaga aaatacccat agctccatac ccggagtcag tttttaaaaa ctgtttaaag
    23401 aaatgcacaa gtattgtgat tgatttttta gttgtttttc ttgatgagaa gctgatgcaa
    23461 aattccgtct ttataataaa aatgatgcca aagcgaacga caaggttgta gttttcacta
    23521 catgtccata cataaaatgg ggtaacattc acgcgcctgg tagcgttacc aacgctacgc
    23581 tcaaacataa tgattctaat aaaacctcag gagactacta tgcctgaagc aacgcctttt
    23641 caggtgatga ttgtggatga tcatccactt atgcgacgcg gtgttcgtca gttactggag
    23701 cttgatcctg gctttgaagt ggtcgccgaa gcgggcgaag gcgcgagcgc gatcgatctg
    23761 gcgaatagac tggatatcga cgtgatcttg ctggatctca atatgaaagg tatgagtggc
    23821 ctggataccc tcaatgcctt gcgcagggat ggcgttaccg cgcaaattat tatcctgacc
    23881 gtatccgatg cctccagcga tgtctttgcg ctgatagacg caggcgcaga cggttatctg
    23941 ttgaaagaca gcgacccgga agtattgctg gaagcgattc gtaccggagc gaaaggcagc
    24001 aaagtcttta gcgaacgcgt caatcagtac ttacgtgaac gtgaaatgtt tggcgcggaa
    24061 gaagatccct tcagcgtgct gacggagcgc gagctggatg ttctgcacga gctggcacag
    24121 gggctgtcaa ataaacagat tgcatcggtg ttgaatattt ccgagcagac agtaaaagta
    24181 catattcgca atctgctgcg taaactcaat gtccgctcac gcgtggcggc caccattctg
    24241 ttcctgcaac aacgcggggc acaataaaaa tagcccgatg gatttatcat cgggctgaga
    24301 tttatgacaa acgcaaaact gcctgatgcg ctacgcttat caggcctacg tggatcgatc
    24361 aatttattga atttacacaa ttttgtaggc cggataaggc gttcacgccg catccggcat
    24421 aaactaagcg cactttgtca tcagtctagc ccgatggcat caccatcggg cctcttttta
    24481 tttactctcc tgcggcgaca aatgttgcat cgcctgcgcg atactacgtt caatcaccgc
    24541 acggcgagta tcgttggcag gtaagagttt caacatcatc tcccacgcgg caacggcttc
    24601 gccaaatcgc tgctgctcaa aggcattaaa cgcatacatg cttagcacac ggatattgct
    24661 atggtccgtt ctcaccagct gacgtagcag ttcaccgccg aggcggttgt cgttgggatc
    24721 agatgaacga gtcaacgctt cagcgtatcc cagtgcggca tcgctgttct tcggatcgag
    24781 gcgatacgca gtagcgtatg catcggtggc gatactggcg ttacccagcg ccatgccaac
    24841 gcggcccaac ataatccagc cttctatatc tcccggattt ttttgcagtt gagtacgcat
    24901 ccccagcgca aggcgcgaca tctcttcttc gttgagcgga tcggctttcg ggtccagcgc
    24961 cctgtccagc aacgccggag cctgtgccgt ggcctgctgc cagattttca cctgctgata
    25021 attgccagtc tggtagtagc tgacgccagc cacaattaac gccaccacaa tacccggcag
    25081 ataaacaaca tatccggcac gcttaccttc cggcacgctt tgttcaggaa acgcttccgg
    25141 caccacgcgt acccgacgcc gcgagcgggc gtatatgacc caaccgccaa tgccaatagc
    25201 cactactggc agcacccaca gcagcacggt cagcggcgtt aacggcggat cgtaagtgac
    25261 gaagttgccg taacgcgcca ccatataatc gacaatctct tttttacttt taccttcctg
    25321 catcagttca tacactttct gacgcaggtc ggtggcaatc atcgagttgg aatcggcaat
    25381 gctgttgttc tggcatttcg ggcagcgcag ttcttcagtg agctgacgga actgttgttc
    25441 ctgtgcttca tctttaaact gcaacacgtc gatggtcgcc agcgctgagc cggagatcat
    25501 cagcatcagc acgcccaata aaaacctcat tgtgcggcct ccttactgta tttctcccac
    25561 agcggcttga tctcttcttc ccagacgcga ggattgagat cgcccgcatg gcgatagcga
    25621 atgataccgt tgccgtcaat aaggaaggtt tctggcgcgc catagacacc gagatccagc
    25681 cctaacatgc cgtcgccatc aaacaggctt agcgcgtaag gattgcccag ctctttcagc
    25741 cagctgattg ccttctggcg atcgtcttta tagttcatgc cgaccacgcg aatgccctgc
    25801 gctgaaagct gattcagata ttgatgttcc gcacggcagg tcggacacca agtcgcccag
    25861 acgttaagca gtactggttt gccctgagtc agcacatccg cctgataaaa ctgccccgga
    25921 ttgtccagtg attcgagacg aaacttcggc acaggcttgc caatgagcgc cgattccaga
    25981 ttggtcggat catccccttc ggcattacgc gccagctgcc acagcagcgc cgcggcaatc
    26041 gccaggaaga taatcaacgg aattaacaat actttgcgct tcatacggcc tccggcgcag
    26101 ttttttgcgg actcacgcgc ttacgatagc gaggatcaaa cagacacagc agtccgccca
    26161 acgccatcat cagcccgccc gcccaaatcc agcgaacaaa tggtttgtag taaagacgca
    26221 cggcccacgc gccgttttcc agctcttcac cgagcgcggc gtacaggtca cgcgtgatgc
    26281 cgccgtcaat tgccgcttcg gtcatcatcg acccggcagt gttgtaataa cgtttttccg
    26341 catacagcac cgtttccggt ttaccctctc gcgttacgcc aatagtcgcc acaccgccgc
    26401 gccagttcgg gccagtcacc tctttgacat cacggaaggt gaagcgatat tcatgaatat
    26461 cgacgctatc gccggacttc atgcgcacat cacgctcaac gctataattc tggctaaagg
    26521 caatgccaac aattgtcact gccagcccca ggtgagccgc caccatcccc caataactga
    26581 aggtggtttt cgtgccgcgt gaaatacgca gcgcagcttc cgcagttgcc agcaccgcaa
    26641 tccagcaggc cattgccagg ccgagcaccg tcatcgccac aactttgctt tcgaacagcc
    26701 acggcaacag cagagacagc accagcgtag agatgaaggc gataatcaat aaattgcgga
    26761 ttttacgtgg gcgatcgcgc ccccagcgca ccagaggacc gacaccaagc agtagcgcaa
    26821 acggcaccat cagccaggta aacatggtgt tgaagaacgg ttcgccaatc gaaatactgc
    26881 ccagtccaag ttgcttgtgc accagcggca gcagcgtccc cagcaacacc accagcatcg
    26941 cggcgaccag caaaacgttg ttcgctaaca gcaaagattc ccgcgaccac agcgcattgt
    27001 ttacgcgtga gcgaactttg tgtccacgcg cggcaaacag cagcagcgaa ccgccaatca
    27061 ccagcaccat aaaggcgagg ataaacatac cgcgcgccgg atcagacgca aacgcgtgta
    27121 ccgataccag cacgccggaa cgcacgagga aagtccccaa cagacacaac gagaaggcac
    27181 tgattgccag cagtaatgtc cacgctttga agctggcgcg ttgttcagtg accgccagtg
    27241 agtgcatcag tgcagtcccc accagccacg gcataaacga ggcgttttct accggatccc
    27301 agaaccacca gccaccccag ccgagttcgt aataggccca tgcggaaccg agcacaatgc
    27361 cgagcgtcag gaaaatccac gccgccagcg tccacgggcg agtaaaacgc gcataagtgc
    27421 tgtccagacg cccgctcagc aaagaagcaa tggcaaaagc aaacgccacc gagaaaccca
    27481 cgtaccccat ataaagcaga ggcggatgga agatcagccc cggatcctgt aacagcgggt
    27541 taagatcgcg cccttcaatc gggaagttcg gcagcgtgcg tgagaacggg ttagaggtaa
    27601 agagaatgaa cagcaaaaag ccgacgctga ccatccccat tatcgccagt acacgggcca
    27661 caatatccag cggaatacgc tgactaaaaa tcgccaccgc aaaggtccag ccgctcatca
    27721 gcagcaccca cagcagaagc gaaccttcat gcgcgcccca agtagccgcc acgcgatacc
    27781 acaccggaag ctgggtattg gagttgctgg caacataggt gacggtaaag tcgttgacca
    27841 cgaaagcatt gaccagcacc agaaatgcgc cagccacaga cataaacagc agccaggcaa
    27901 acaagcggga agacgccatc atgcgcgcat ctccgcgcgc cacgccccat agcggataca
    27961 cggacagcag cagcgcaatc cctaacgcca agcacagcag cccgtttcca atttccggca
    28021 tcatgatgct gggtccttat aaacactcgc cgggcgacgg tggttagctt ccatcgcttt
    28081 ctcaacttct ggcggcgtgt agttttcgtc gtgtttcgcc agcacttctt tcgcgaggat
    28141 atgattgcct ttttccagct cgccctgcac cacaacgccc tgcccttcac ggaacagatc
    28201 cggcaaaatg ccttcgtaag agacatccac tgagccttca gcatcgtaaa tggtgaaggt
    28261 cactttcagc gaattgggat cgcgctgcac gctacccggc atcaccatcc cgccaacgcg
    28321 cagacgctga ccgacttccg gcatctgctg agtttcacgc ttgccgtaga gaatttcccc
    28381 cggcgtataa aagagatcga tattcgagcg cagcgcatat agcaccagac cgatagtcag
    28441 cgctagccct gccaacacgg cacaggcaat ccacaagcgg tttttacggc gaatattcat
    28501 gcagcctcct gctgttgcgc agcacgtaaa cgcgcctcac gcgcccgctg ttgcgccaca
    28561 ccacgcagaa ttgcgcgatg ttgcatcacc gagtgcacga ccaaaaccac cagcggaata
    28621 acggtcatca ccaccgccag ccagacaaaa aaggcgtaac cgcccattgc gaaaaattca
    28681 ttccaggaag caaatgcagg ggtcatttac ggcctctttt cagtatcagt tcactcaccc
    28741 acggacggcg tttttccatc agcaaaatca aattacgcat ccgcatcagc gtcagcgtgg
    28801 cagacaggag caggaagcca aaaatcgacc agcgcagcgg cgaacgcatc gccggatcga
    28861 tactttgctg catccgcgtt gatccctgat gcagggtgtt ccaccactcc acggagtaat
    28921 gaataatcgg cagattcacc acgccaatca gcaccaggat acctgccgca cggcccgcca
    28981 gacggcggtc gtcgaaggcg tgccacaggg caatcacacc cacatacaaa aacagcagca
    29041 ccagttcaga agtcagacgt gcatcccata cccaccaggt gccccacatc ggttttcccc
    29101 atgcagagcc ggtaaccagg gcaataaagg taaacacggc accaatgggg gccatcgccg
    29161 ccaccgccag gttggccatt ttcatctgcc agacaaggcc aataaacgcc gccaccgcca
    29221 ttgatgcata aatgcccatc gaccagatcg ccgcaggcac atgcaggtag ataatgcggt
    29281 agctatttcc ctgctgataa tcagccggag caaagccgaa tccccagatc cagccgacgg
    29341 taagcacgac cacactggca attgccagcc acggtataaa ccagccacag atttgataca
    29401 gccgtggtgg gatcgccagt tgatgcagtg ttttccacat agtttcgata ccagactcga
    29461 acaaaaatca gtaatccagc gttattgaat gctgattcgt aacgctgccg ccgtcgcaaa
    29521 aggacttaat gtcgcggtgc ctgccagcaa cgcgcctaaa attgccagat acccatcaac
    29581 gggcaaatgc atagaagccg cgtccatcgc ggcggtggca aagatgagta atgggatagt
    29641 cagcggtaac accagtatgc tgagcagcac accaccgcgc ttaagtccca ctgtcagcgc
    29701 cacgcccggt gcaccgagaa agccaagcgt aggcgttccc agcagcagcg tcagcgccat
    29761 cacttgccag ccataaacat ccattcccag tagcattgct accagtggcg aaaggatgag
    29821 taacggcaga ccggttacca tccagtgcgc catcaccttc gccagcacaa cggcgggcaa
    29881 gggtaacggc aacaacatca attgttcaag actgccgtcc tgcaaatcgt cacggaacag
    29941 tcgttccagc gccagcaagg atgaaagcag cgcagcaacc cagataatgc ccggtgcaat
    30001 acgcgccagc agttgcggct ccggaccgat actgagcgga aaaagggtaa ttacaatcag
    30061 gaagaaccac agcgggttgg cgatttcggc gctatggcga aacgctacgc gcagttcaag
    30121 acggaaaata cgccagaaca tcatgcggcc ctcgtttgcg tcagtgaaat gcggcgaatt
    30181 ttactttcag caacgttgag cggctggtgg gtagtcagaa tcacaatccc cccctgctcc
    30241 gtatgctgcg ccatacgctg ggtcagacga tcgacgccgt taacgtcaat cgcggtaaaa
    30301 ggctcgtcga ggatccataa tgtggcacgg gtcaaccaca gacgcgctaa agcgacgcgg
    30361 cgttgttgcc cggcagaaag ttgatttacc ggaatatctt cgaatccggc aagcccggcc
    30421 tgcgccaagg cttccagaca ttgtgcggca tcgccatcgc gatgataaaa gtgcagattt
    30481 tctaacgccg tcagccgggt tttgatcccc ggctgatggc ctatccataa cagggtttga
    30541 tggtagctgt cgcgtacctg atgcaagggc tgcccttgcc agagaacctc gccagcgtca
    30601 gggcgagaca accccgtcag caaacggaga agcgttgtct tccccgcgcc gttgctaccg
    30661 gtgatttgta cccactctcc tgcgttcagc gtaaatgaca agccactaaa taaggttcgt
    30721 tcatcccgct cacaaagtaa ctctctggct tcaagcatac ccacgcaata accctgttaa
    30781 aaacctggct cgacttcacg catatcgggc agcttgtgcg cgatcccttt atggcaatca
    30841 atacaggttt gcccatcttt caccgcctgg tcatgcatct tcgcggcaac cgatttctgg
    30901 gcggttgtat ccatatactc gaagttgtga cagttacggc actcctgcga gttattgtcc
    30961 ttcatgcgcc gccactcatt ctgtgccatc gtcagacgat gagcttcaaa tttctgcggc
    31021 gtgtcaataa cgccaaaaat tttaccatac agctctttac ttgctttgag cttgcgtatc
    31081 atcttcggca caaactcgtg cggaacgtga caatccggac aggtcgcacg gacgccgcta
    31141 cggttgttgt agtgcacgga atccatgtat tcctgataca ccgtgttgcg catttcgtgg
    31201 cagctaatgc agaactcttc ggtattggct ttttccatcc cggtgttaaa gcccccccag
    31261 aagacgatgc cgccaacaaa accgatcaac agcagcgtcc ccagcgccag atggctgggg
    31321 gtacgccacc atttccacag gcgcttaatc agaccaggct tacggtcaga atttcccata
    31381 ataacctctt atttcccgta accttttgat ggggtaaagg tattccccac gattggcgcg
    31441 gtatcggcct gcggtacgtg acattgcaga cagaaataac gacgcggagc cacttccgcg
    31501 cccactttgc cgtcgctgtc cataaagtga gtaggactga tacgcggcgc gccagtggtg
    31561 cgatagcttt cgacaccgtg gcactgcaag cagcgattgg tattggtcgt tacctgataa
    31621 ccttcaacgc tatgcgggat cattggcggc tgattcacat agttcagcgg catccgatcc
    31681 tgctcttttg gcatccgaat ggccccttcc tgcgtcccgg atacttccgg cgactggcta
    31741 aaatcgacgc cattagccgc ccaaaccgcg ccgcttacca ccagggccag catcgccgtc
    31801 cattgacaca gcgctttctt caggtcatgg cttttcatga tttcgctccc gaactccatc
    31861 gtgtagttat tgtaaataca tcctcagaac agacatccac gcagcgaccg cacgtcatgc
    31921 aatcgcggct ggtgacctgc accgggcttt gttcatccag caccggggca cgtagcacat
    31981 gcggttccgg gcaaacatga aaacaatcca tacagcggtt acatttctgc cgatcgctgg
    32041 ccgcaacggt aatcacacct ttgctaccca gcacgccata cagcgcgcca accggacaaa
    32101 tgtgcccgca ccagccgtgt tcaacgacca gtagatcaaa taaaaacagc gcgagaataa
    32161 gcagcgcgcc gctgccgaag cccattacca ggctacgccc catcagagaa acggggttga
    32221 tccattccca aatgagcgtg ccagttaagg ctgatcccac cagaatgacc accaacagca
    32281 cgtagcgaat gtggcgaggg atcgtcgcag actgattgag gtcaaagcgc ctgcgtaacc
    32341 agttcgctaa gtcggtaatc gggttcagcg ggcagaccca gctgcaaaat aatcgctttc
    32401 ccgccagggc gtagagcacg gtgataatca ccgcaccggt caacgccacc gtggcaggca
    32461 gatgaccact ggcaagactt tgcagcgtca tcagcggatc ggtcagcgga acggtgtcga
    32521 ataacaggct actgctgtag ttgccgtgca agatccacac accaaaccat ggaccgctca
    32581 aaaacatccc cagcacgaag aactgacaaa ggcgacgtaa caccagccaa cggtgactgc
    32641 gccaccagcc ttttttctcc agcgcctcgc gcccggcgtc acgtttacga tttgccattg
    32701 ttcccctcca gccagccgaa gcggtaatgg tgacctaact cccctttcgc cagtgacagc
    32761 ggtaacacct tgattgccgg ttgttccagc acgcatactt tttcgcactt accgcaaccg
    32821 gtacaggcgt cgctgtgaac cgtcggcaga aagcgggcgt gcttaccggt acgcgtgttg
    32881 cgctccagct ccaaggtgat ggcctcatca attttcgggc attcgcgata acaaacatcg
    32941 cagcgcagcc cctgaaagtt gagacagttt tcctggtcca ccagtaccgc cagccccatc
    33001 cgcgcgtcgt cgatcgattc aatctcacga tccagcgcac cgcttgggca cactttggcg
    33061 cacggaatgt cctcacacat ttcgcaagga atatcccgtg cgacaaaata tggcgtgccc
    33121 gccgacagac cagaggccag cgtcgccagt tttaaggtgt cgtaagggca agcctgaaca
    33181 cactgaccgc aacgcacaca ggcactggca aaggcgttct cgtttatggc tccgggcggg
    33241 cgcaaccgca cgccagatgc gcgtgcggtt tgctgttgta accccagcgc cacacccacg
    33301 gcagccagcc cgcctgctgt gcgaacgaca tcgcgcagaa agcggcggcg accattttga
    33361 ggtttcgctg accgggacat aatggcttac accttctcca gtttgaccgc gcacttcttg
    33421 aagtccgtct ctttcgagag cggatcggtc gcatccagcg tcagtttgtt aaccagctgt
    33481 gcggcgtcga agaacggcat gtacaccagg ccctgtggcg gacggttacg accgcgcgtt
    33541 tcaacaatcg agatcacttc gccacggcga gaaaccactt tcactttgtc gccacggcgc
    33601 agatcgcgcg ctttcgcatc cagcgggtga ataaacaaga ccgcttccgg gaaggcgcgg
    33661 tgcagttccg gcacacggcg agtcatactg ccggtgtgcc agtgctccag aacgcgtccg
    33721 gtagagagcc acaggtcgta ctcttcatcc ggtgcttccg ccgccggttc gaacggcagt
    33781 gcgaagatca ccgctttgcc atccggttta ccgtagaact tatagccttc gcccgctttc
    33841 acgtacgggt cgttaccttc gctgtaacgc cactgtgttt ctttaccatt caccaccggc
    33901 cagcgcagac cgcgcgcttt gtggtagtca tcgaacggtg ccagatcgtg accgtgaccg
    33961 cgaccaaacc atgcgtactc ttcgaacagc cctttttgca gatagaagcc cagctcgcgg
    34021 gattcatcgt tcagctgatc ttccgccagt tcggataccg ggaatttgct cacttcgggg
    34081 gtggcataca gaacttcgta cagcgttttg ccacgcagtt ccggtttctt cgccagcagc
    34141 tcttccggcc atacttcttc agttttgaag cggcgggaga actggactaa ctgccagaga
    34201 tccgatttcg cttcgcctgg cgcctgtacc tgctgacgcc agaactgagt acggcgttcg
    34261 gcgttaccgt aagcgccctc tttctctacc cacattgcgg tcggcaggat caagtcggcg
    34321 gccagcgcac tgactgtcgg atacggatcg gagacgatga tgaagttgcg cggatcgcgc
    34381 cagcccggca tacgctcttc attgatgttc ggcccggcct gcatgttgtt ggtacacatg
    34441 gtccagtaaa cattcagctt gccgtctttc agcgcacggt cttgtgccac cgcatgaaga
    34501 ccgattttcg ccggaatggt gccgctcggg atattccact tcttctcaca gatatcacga
    34561 tgtttctcgt tagttaccac catgtccgca ggcagacggt gagcaaaggt gcccacttca
    34621 cgcgcagtac cacacgcaga aggttgcccg gtcagggaga acggaccgca acccggctgg
    34681 gaaattttgc cggtcagcag gtgcaggttg tagaccaggt tgttagccca cacaccacgg
    34741 gtatgctggt tgaagcccat cgtccagtag gagatgactt tcttgttcgg atcggcatac
    34801 agctgcgcca gttgttctaa ctggtctttc ggcacgccgg tcatttcagc ggttttttcc
    34861 agcgtatact cagcaacgaa ggctttgtaa tcttcaaagc tcatcggttc ggaggcgtca
    34921 gaacccggat tcttcgctgc tttttccagc ggatgggtcg gacgtaaacc gtagccgatg
    34981 tccgtcgccc ctttgcgcag gttaacgtgc ttgctgaaga agtcctgatt tatcgcattg
    35041 ttttgaatga tatagttggc gatgtagttc aggatcacca ggtcagattg cggcgtaaag
    35101 atgatgccgt tatccgccag ctcgaagcta cgatgctggt aggtagaaag caccgccacg
    35161 gtgacgttct ggttagagag acgactgtta gtgatgcgtg accagaggat cgggtgcatc
    35221 tccgccatgt tagcgcccca cagcacaaac gcgtcagcct gctcgatgtc gtcatagcag
    35281 cccatcggct catccatacc aaaggtacgc ataaagccaa ctactgccga cgccatacag
    35341 tgacgtgcgt tcgggtcgat gttgttcgaa cggaagcccg ctttgaacag cttggacgcg
    35401 gcataacctt cccagatagt ccactgacca gaaccgaaca taccgatcga ttccggccct
    35461 ttttctttca gggcggtttt gaatttctct tccatcacat cgaaggcctg atcccaagtg
    35521 attggggtaa attcgccttc tttgtcatat ttaccgtttt tcatacgcag cagcggctgc
    35581 gtcaaacggt ctttaccgta catgatcttg ggcaggaaat agcccttaat gcagttcagg
    35641 ccacggttaa ccggtgcgtc cgggtcgccc tgacaggcca ccacacgccc ctgctgcgtt
    35701 ccgaccagaa cgccgcaacc agtaccgcag aaacggcacg gcgctttatc ccatttaatg
    35761 gcttcctgct gaccaacaac ggcgcgggca acgcccggca cgctgagacc ggcagccgcc
    35821 gcagcggccg caacggcgtt agctttcata aagctacgac gactgagttt catggtgttt
    35881 cctcaccttg ctcttcctgc tggtgataaa ccagcgacac cgccagcacg ccctctacgt
    35941 tgcgtactga ctcaatggtt tggatcagcg tttcgctgtc ttctgcttcc accaccacaa
    36001 tcaactgacc gctcggcgcg tcgctgacag caacttcaca gccgggaaag gcgttcagtt
    36061 gggtgctgat gtctgaaatt cgttcgcttt tggcctgcac gaccaggctg caaacttgcc
    36121 agttagtgtg catggagata ctccgcagtt atggctgata ccggacagct ggctgcacat
    36181 gctccacatc cgttgcaaag ttggctatta agttgcgcct ggtagatccc ggagagcgtc
    36241 gggcgaaaga taattgccat tggctcacag ctatcctgac agcggcggca ttcaactgac
    36301 tgatacgcca ggcacgcgtc cccgatggtg aactgcaaat cccaggccct ggtgtggcgc
    36361 ggagaaaata gcgattcggg gcaagcctgc gcgcaggcgt aacagaagct gcactcgtta
    36421 tttttgaaat taacgctcgg atagccaccc gtgccgcgtt gcagaatgtt gttttcacaa
    36481 gcattaatac aggcgtcgca gcgggtgcaa tgggtcagaa aatgagattc atcaccggac
    36541 cagggcggac ggataccgtt actggctttg cgccagcaac cagtgagtat gccccgacgg
    36601 gatgcatcaa tcttcacatt gaccttccat cattaacgcg ctctgaaaat tgagagcgac
    36661 caaatagacc gcataattaa taagccattt ttatagccgc taagatatta aaggatgtgt
    36721 caaagatgca taccccgatc ggggtaaaac ctgtagcagg atcaaaagag tggcgggaag
    36781 cgtggcaaaa acgggctttt gctcacattt caaatggtta taaacatatt tatatagcga
    36841 ttaattcacc agagatattt ctgctggttt gctccctcat cagaatttaa cactaaaaga
    36901 gcaggtaaaa ttgtctgaat gttctttaag ttattcataa agcaaattaa taaatctgat
    36961 gaatatgtta accttcagcg acatcatcgg tgaaaaccta taaatgaaga aggaaagcaa
    37021 aaaaatgaag accattctac ctgaagtatt gtttgccgct ttcgctacca cttccgcctg
    37081 ggcggcagaa agcgtccagc cactggaaaa aatcgcgcct tatccacaag ctgaaacagg
    37141 catgaagcgt caggtgattc agttaacccc gcaagaagat gaatctaccc tgaaagtaga
    37201 actgttaatc ggtcagacgc tggaagtcga ttgcaatttg catcgtctcg gcgggaagct
    37261 ggaaagcaaa acgctggaag gctggggcta tgattattat gtctttgata aagttagctc
    37321 gccggtttcc acgatgatgg cctgcccgga tggcaagaaa gagaagaaat ttgtcaccgc
    37381 gtatctgggc gatgctggaa tgctgcgtta caacagcaag ctgccgatcg tggtgtatac
    37441 gccagacaat gtagatgtga aataccgcgt ctggaaggcg gaagagaaaa ttgacaacgc
    37501 ggaagttcgc taaactgttg tgaagtacgg caccccgttg gttggataag gcggttacgc
    37561 cgcatccaac atctaacgcc cgagccggtt gcctgatgcg acgctggcgc gtcttatcag
    37621 tatacgccac atccggcata ccatgccgga tgtggcacat cattacaacg caatatccgc
    37681 cacttctttt tgaacgggtt gcggtttcaa ctgcggtttc ggcgtactat ctgctgcttg
    37741 cggtttgtcg tatttcagcc ccagcacttc gctggtgtac tgcaactcgc gttctgttgc
    37801 cgctacatca ccgttcagct tgcgtccata agacggaacg atcgctttca acgtagcctg
    37861 ccattgcggg ctggaaacgc gatcgccaaa tactttttcc agtagattca acataatcgg
    37921 cgcggcggtt gacgcccctg gcgatgcccc cagcagcgcc gcaatggtcc cttgctggtc
    37981 actgaccact tcggtaccca gacgcagtac gccacctttc tcggcatcac gcttgataat
    38041 ctgcacacgc tgccccgctt gccacaaacg ccagtcctct tttttcgctt gcggatagta
    38101 ctctttcaac gcttcaaaac gatcctcttc actcaacatc acctgactca ccagatattt
    38161 caccagatcg aaattatcca gcccgacgtg catcatcggc atcacgttag aggtggtggt
    38221 ggaactcatc agatcccaca atgaaccgtt tttgaggaat ttggttgaga aggtggcaaa
    38281 tggcccaaac agcactacgc gtttaccgtc cagaacacgg gtatcgatat gcggaaccga
    38341 catcggtggt gcgccaacgg atgctttacc gtaaactttc gccagatggc gattaaccac
    38401 gtctggattt tccgaaacaa ggaactgtcc gcccaccggg aaaccggcgt agtctttcgc
    38461 ttccggaatc cccgattcct gtaacagctt cagcgccgca ccgcccgcgc cgataaagac
    38521 aaatttggcg cggatgttct gtgcagtgcc gtttttcaga tcggcaacgg taacggtcca
    38581 ggtgttatcg tcattacgct ttagggcgcg gacttcgctg ctgagttgca gcgagaagtt
    38641 agatttcttc tgtaaggaag caattaactg gcgggtgatc tcaccatagt tcacatcagt
    38701 gccaatttct gttgcggatc gcgcccttcc atcactaacg gtgcccactc tttgatctgc
    38761 gcgtgatctt cagagtaacg cataccgcga aacagcgagc tttgttgcaa cgcggcgtaa
    38821 cgggcgcgca ggaaattaac gttatcctcg ccccagacaa agctcatatg cggaacggta
    38881 ttgataaatg aacgcggagt acgcagcacg ccgcgctcga cctggtgcgc ccagaactgg
    38941 cgggaaatct gaaatgcttc gttaatggcg actgcttttt caatactgat gctgccatcg
    39001 gcgttttgcg gggtgtagtt cagttccatc agtgcagaat gcccggttcc ggcgttgttc
    39061 tagccgttcg aactctcctg cgcgcccccc tccaggcgct ccaccatggt catcgaccat
    39121 tcaggctcca gctcgcgtca ataggtcccc aacgtggcgc tcataatgcc gccgccaatc
    39181 aacagtacat cagtttcctg ctcctcggac gctttcgctt ttgccgccat cgaaacggca
    39241 ttaagcccca cggccatcga gaagagcatg gcagtcactt ttttcatctt gttaatgcct
    39301 tacttttagt cgctttattg caggtgagat ttgcgcggca tcaacggtaa cacttaagta
    39361 acaacattta aataatgttt ataaattata ttctaatttt agaaacatta taatttcgtt
    39421 gattaattat agggttatta gaaatgaagg gatttttagc atattgacct gacggcagca
    39481 gtccgccagg tcagcgtcgt aatattatgc cgtccgggca acggcatcac gcgaagcggc
    39541 atcgcgctct tcgcccgtca gctcgctaag ttgcccattg cgcatttcca gcaggcggtc
    39601 ggcgtggata aagtaatgat catcatgact gatagcgaaa atagttttac ccatctcctg
    39661 catcagcggc agcaacacct gataaaactc acgacggaag tgtggatcct gatccaccgc
    39721 ccattcatcc agcaggataa tatcgcgttc ttctgccagc gccagcaaca gcgccacgcg
    39781 ttttttctgc ccttttgata acttcaggtt aacaatacgc ccgttgctta actcaagctt
    39841 atgagccatt ttcagctgcg ccagccactt ctcaaccagt tgcgggttag cgggttgccc
    39901 ctccggcccc agcagttgat caaacagcca gacatcggta aacactgccg aaaacagttt
    39961 gcgataatct tccggttgtt cgccgctgac aggtttgcca tccagcaaga tttcgccgct
    40021 ttgtggctga tacaagcccg tcaacaacat cgccagcgtc gattttccgc taccgttacc
    40081 gccaatcaga aacagcagct cgccacgttt gatggtgaga ttaatcggac caacggaaaa
    40141 cgcgttatcc tgataagcaa acgtcacgtt acgcagctcc agcgtttgcc agttgggaaa
    40201 cgcctgcggg cgcggaaact cagctttgaa aggcgcgagc gcgaatttgt tcagcttgtt
    40261 aaacgccacc tgcgccgtca gcagcgtcgg caatgcgcca accgccgaaa gcagcggcgt
    40321 acgcaggaat aacagcgtca acgaataggt cgcagcaacg ttggtatcag cccaaccgag
    40381 actgttcgcc atccagaaca ccaggccgat agcgcccagc atcatgatgt ttgaccagtt
    40441 cacggcacta agatggaagg tgtctgcgcg aataatatga tggcgatact cttgcgcatc
    40501 ggggatgtag aggttgttaa acacatactc ggcgcgttcc cggttcagag tcagctcttt
    40561 gcgcccttca agtacagttt gaaaatccgt gtacagcttg tcttcggttt cacgtagggt
    40621 cgccatatgt ttgtacaccc gcgccaccag cacaaaaccg ccccagatgg tgatcgccat
    40681 ccagatagcc gttaccagca acattttgcc cgacagcatc cacagatacg ccgatgaacc
    40741 gatagtgaga atgatcccct gccccagttc cggcagacgc acaaaagcaa tggtgatatt
    40801 gcgcacatcg ctggttaatc ccgccagcaa cgaggcgcta ccgagttgtt caatgcgctc
    40861 gacgtgggta tccagaatcc gcttgataaa ttcactacgc agtcggtaaa cgaagtgatg
    40921 ccccaaagtg gtgagcgcca gttgcgatcc gagagtgact gccatcaaca gcagcaataa
    40981 tcccagaaac tccggtaaca ccagcagact ggtatccgcc gtttcgataa ggcgctgatt
    41041 gataaaagca attaagccaa tgcctaatgc cgcactggcg aggcttagcg ccatcacact
    41101 gataaatggc cagcgatact gccgccagac aagtacaaga agttccatgc agaaaacccg
    41161 gacaatgaat tacagcccgc agtttaaaca tcttcgcgcg cacagcaata ataattctta
    41221 tttttattct tttttacctg cctgacggaa tgtcaggttg tagcggcagt cggtggtgag
    41281 tggatgaaac cccgctttca acggttgaat accgtgataa aacagccgcg attcaccgcc
    41341 ccataccacc acatcgccat gttccaacaa caaacgtttg agcggatcat tacgtttcag
    41401 gccaccaaat tgaaaaatcg cgggtaagcc cagagaaaca gaaacaattg gcgcgcgcag
    41461 atccggttcg tctttatcct gatgcagcga cagtttcgcg ccaggagcgt agcggttgat
    41521 aagacaagca tctggctgga aatctggata gcccgccgcc gtagccgcac gttgacataa
    41581 atcatgaaag ctttgtggca tggcgggcca cggtttattt gtttgcggat caatgggcga
    41641 atagagataa ccttgccgat gggtcgtcca gcccagatgc ccacagttgg tcatcgccac
    41701 cgacatggta tatccgcctg gagcgaccat ctggcgaaac ggcgactggc tggcaacgtc
    41761 attaatatcg cggattagtt gctccgcagc attaaaagca aaacgccgta aaattaccgc
    41821 accagccgcc agtggctctt gccacggttc agcatcggca aacagatcca acattacctc
    41881 tcctcatttt cagcttcgcg gcgcagcagt tgcgctttac gcgacacgcc ccagcggtaa
    41941 ccggaaagtg tgccatcacc acggaccact cgatgacagg gtattacgat agccagcttg
    42001 ttggcggcac aggcgctggc aacggcccgt accgctttcg gtttgccgat ggcgttagcc
    42061 agttgctgat aactgacggt ttcaccacaa ggtatcgtgc gcagtgcctg ccagacttgt
    42121 tgctgaaaag cagtgccgcg aatatccagc ggtaacgtca gcggcgtatc gcgttgattg
    42181 aggctggcga tcacttcacg cacatgttgc tgaaacgtca gatcggcagg cgcgttgtcg
    42241 gcagcgggaa acatctgctg caactcgctg attaatgtcg cgtcatcatc gcccagcaat
    42301 atcgcgcaaa tcccccgctc gctttctgcc accaggcaac gacccagctc acaatcagcc
    42361 agcgcgtaac gcaccgccag attttcgcca ccgtgacgga attgtttagc cgtcatgccc
    42421 agcgtttcat cggctttgcg gtagtaactg ctgctgtcgg ggaatccggc gttaagaata
    42481 gacgtcgtca cgctctcccc tttcgccagc gattcgcgca aacggcgagc gcgccaggcc
    42541 tgttgccagg ctttaggcgt cattccggta gtagctttaa acaaccgatg tagatgaaat
    42601 ggactcatcg ccacctggtc ggctaaggct tccagcgtta caggcgtttc ctgttccagc
    42661 agtcgacacg cgtgggtgat tttatccaac cgatgttgcc ggggattggc tttgtctggc
    42721 tgacaacgtt tgcaggggcg aaatccggcg gcgagtgcct cgctggcatt tgcgtagaag
    42781 gagacatttt cccgcaaggc atgtctggcg cggcaagaag gacggcaaaa gatacccgtg
    42841 gtgcgcacgg cgaaaacgaa ttcgccgtcg gcattcgggt cgcgggctaa gacagattgc
    42901 cagcgttgat cgtcagttaa gcatgtggcg tttttcataa tcagctccct ggttaaggat
    42961 agcctttagt ctgcccggtc accatcacgc aaaaaccaac aatcttgcgc tttaattttt
    43021 ttcgctgaca aggaagcttt taaactgtgg tgacatccag gttttaaagc tatcgccttc
    43081 tttggtgatc atataaaccg ccagcccttc ccggcgaaca acctctttgg ctttctccgg
    43141 cccgagtacc atcaagccag tatcccaggc atcggcttcc agcgccgtcg gagcaatcac
    43201 cgtcacggat accagattgt gttcgatggg acgcccggtt tgcggatcga taacatggga
    43261 aagacgtttg ccgtccagtt cgtaatagtt acggtagctg ccagaggtgc taatcccatg
    43321 accgttgata tccaccacag cctgaaccgc gttttcttta tcggttggtt tttgaatcgc
    43381 tacccgccac ggctggcctt caccgttcat accacggctg ttcagcgcgc cgcccaccga
    43441 caccagatag cgggaaatcc cttcctgctc catcaagcgt gccaggtgat ccgccgcata
    43501 accttcaccg acggtggaga gatcgacata taaatccggc aggtcttttt gcagatattg
    43561 ctgatgcgac tgattgatga ccgtcaggtg ctgtaagccg gttttggctt tcatcgcatc
    43621 gatctgttcc tggctcggaa tttgaaccgg ctgttgttcc gggccaaagc cccacagatt
    43681 caccagcggc ccgacggtaa tatccatcgc accatcggtc cttgccccaa tgcgcagcga
    43741 ggtggtgacg atatcggcca tcgcttcact taccggccac ggcgacaaac tttgcgagtc
    43801 gttaaagcgc atcagcgcgg aatctttttt ataggtcgaa agcagctgat cgtcggcgtc
    43861 cagctgggtc tgaatctttt ctttaagttc ggcactgcgt ttggcatcaa tgcccgggat
    43921 gctggcacgc cagaaggtac ccatggtttt gccttcaaga acggtaactt cagtagcgtg
    43981 ggttttggcg ggttgtggtt tttgatcgca accaacaaag aagagcgcgg caaccagcag
    44041 tgccacgcgg gtaaagctta tttccatacg tgattatcct catgcgaacg gtcgcaagag
    44101 tacaccaaaa aactgtgttt gtacgctgaa aacaatgaaa aaagggcccg caggcccttt
    44161 gttcgatatc catcgagatt agaactggta aaccagaccc agagctacga tgttatcagt
    44221 gttgatgcca gcgtcacgag tgaactggtt gtcgtccagc aggttgattt tgtagtcaac
    44281 gtaggtggac atgtttttgt tgaagtagta ggtcgcacca acatcaacat atttcaggat
    44341 atcttcgtcg tcgtagttac gaccattgat gacacccagg tttttacctt tagactgcag
    44401 gtaagccagg gacggacgca gaccgaagtc gaactggtac tgagcaacag cttcgaagtt
    44461 ctgtgctttg ttcgcccaac ccaagttgcc tacgcgagtt gcgttgtagg tctgggtgta
    44521 ctgagcagct aggtagatgt tgttcgcgtc gtatttcaga ccaccggtgt aggtttcagc
    44581 gcggtcgcca ttaccgatgt aacgctcatc ataacggttc agaccgaagt tctggtcatc
    44641 agtacgtttg gagctggaaa ctgcagcacc gataccgaag ccttcgtaat cataagtaat
    44701 agaaccgcca acgccgtcac cgttctgacg cagagcttca cgaccgttgt tggtcatgcc
    44761 ttcaccttcc gggctaccgt ttttgccctg atactgaaca gcgaagttca gaccgtcaac
    44821 cagaccaaag aagtcggtgc tacggtaggt tgcgaagccg ttaccacgct gctgcatgaa
    44881 gttgtcagaa ccgtaagtgt caccaccgaa ttctggcagt acgtcggtcc aggaagttac
    44941 gtcgtaaact acgccgtagt tacgaccgta gtcgaaagaa cctacatcct ggaatttcag
    45001 acctgcgaat gccacacggg tccaggagtt gttttcgttt tcagcgctgt tgccctggat
    45061 ctgatattcc cactggccgt aaccggtcag ctggtcagta acctgagttt cacctttgaa
    45121 gccaagacgc atgtaggtct ggtcgccatc tacagacttg tcgtcagaga aatagtgcag
    45181 gccgtctact ttaccgtaca gatctaattt gttgccgtct ttgttgtaaa cttcagcagc
    45241 gtttgctgcg cctgctacca gcagagctgg gaccaggagg gacagtactt taactttcat
    45301 gttattaacc ctctgttata tgcctttatt tgctttttta tgccactgca tactgattaa
    45361 ccctcattaa tcagtcggca agtccattct ccccaaaaat gcagaataat ccaacacgaa
    45421 tatgatacta aaacttttaa gatgtttcat ttatcgctat agatgtttca aaatgtaaat
    45481 acaagggaac tttttaagat tattgcggaa tggcgaaata agcacctaac atcaagcaat
    45541 aataattcaa ggttaaaatc aataacttat tcttaagtat ttgacagcac tgaatgtcaa
    45601 aacaaaacct tcactcgcaa ctagaataac tcccgctatc atcattaact ttatttatta
    45661 ccgtcattca tttctgaatg tctgtttacc cctatttcaa ccggatgcct cgcattcggt
    45721 ttttttaccc ttctttacac acttttcatt attctgtgct accacagaaa aactataacg
    45781 cttgttaact atttcacaaa taattaacat cagcataatt tccagcaatc tttgtttatt
    45841 tgcaattaat ttcgttgggc tttttgtagg ttatttgtac agcaaaatgg cgcttgtaca
    45901 tctatttccc cccatacagg atgataaata tcacgggaga atagagaatc atcaatcagg
    45961 taagagtctg gaatttcaca ctgtaccctt tatactgccc tatcacttcg cgaagtttta
    46021 acaggtcata aacacgaatg cgtcagaaag agacaacggc cacgacccgc ttttcactcc
    46081 taccggggag cattacccgc ttctttttac tgttgatcat tgtgttactg gtgacgatgg
    46141 gggtaatggt acaaagtgcc gttaacgctt ggctgaaaga taaaagttac cagattgtcg
    46201 acattaccca cgcaatccaa aagcgcgtcg atacctggcg ttacgtgacc aggcagatct
    46261 acgacaacat tgccgcgacg acctccccct cctccggcga aggtttacaa gagacgcgcc
    46321 tgaaacagga tgtctactat ctggagaaac cacgccgcaa aacggaagcg ttaatctttg
    46381 gctctcacga caactcaacg cttgagatga ctcagcggat gtccacttat ctggatacat
    46441 tgtggggtgc agaaaatgta ccgtggtcga tgtattacct gaatggtcag gataacagtc
    46501 tggtgctgat ctcaacccta ccccttaaag atctcacctc cggatttaaa gaatcgaccg
    46561 tcagtgacat tgttgattca cgtcgtgcag aaatgttaca acaggccaac gccctcgatg
    46621 aacgcgaaag tttttctaac atgcgccgcc tagcctggca gaacggtcat tactttacct
    46681 tgcgtaccac gttcaaccag ccgggacatc tggcaacggt cgtggctttt gatctgccga
    46741 ttaatgattt gatcccaccg ggtatgccgc tggacagttt ccgccttgag ccagacgcga
    46801 cggcaacggg aaacaatgat aatgagaaag aagggacgga tagcgtcagt atccacttta
    46861 acagtacgaa gattgaaatc tcctcggcac tcaactctac cgatatgcgc ctggtctggc
    46921 aggttcctta tggcacctta ttgctggata cgttgcaaaa cattctgctg ccactgctgc
    46981 tgaacatcgg tttgctggcg ctggcgttat ttggctatac cacattccgc catttctcca
    47041 gccgcagtac agaaagcgtc cccagcacga cggtcaataa cgaattgcgc attttacggg
    47101 caatcaatga agagatagtc tcactgttgc cgctcggcct gctggttcac gatcaggaat
    47161 cgaaccgcac tgtcataagt aacaaaattg ccgatcattt gctgccgcat ctgaatctgc
    47221 aaaacatcac caccatggcg gaacagcatc aggggattat tcaggcgacg atcaataacg
    47281 agctgtatga gatccgcatg ttccgcagcc aggtcgcgcc gcgcacacaa attttcatta
    47341 ttcgcgatca ggatcgcgaa gtgctggtaa acaagaaact caagcaggcg cagcgtctgt
    47401 atgagaaaaa ccagcagggg cggatgacct ttatgaaaaa cattggcgat gcgctgaaag
    47461 aacccgcaca gtccctggcg gagagtgcgg ctaaactcaa cgccccggaa agcaaacaac
    47521 tggcgaatca ggcagatgtg ctggtgcggc tggtcgaaga aatacagtta gcgaacatgc
    47581 ttgcggatga tagctggaaa agtgagacgg tgctgttctc cgtgcaggat ttaattgatg
    47641 aagttgtgcc ttcagtgttg cctgccatca agcgtaaagg tctgcaactg ctgattaaca
    47701 atcatctgaa agcacacgat atgcgccgcg gcgatcgcga tgccttacga cgtattttgc
    47761 tgctactgat gcaatatgcc gtgacctcaa cgcaattggg aaaaatcacc cttgaggttg
    47821 atcaggatga gtcctctgaa gaccgcctga cattccgcat tctggacacg ggagaaggcg
    47881 taagtattca tgaaatggat aatttgcact tcccgtttat caaccagacc caaaacgatc
    47941 gttatggaaa ggcggacccg ctggcattct ggctgagcga tcaactggca cgtaaactgg
    48001 gcggtcattt aaacatcaaa acgcgggatg ggcttggtac acgctactct gtgcatatca
    48061 aaatgctcgc cgctgacccg gaagttgaag aggaagaaga gcgtttactg gatgatgtct
    48121 gcgtaatggt ggatgttact tcggcagaaa ttcggaatat tgtcactcgc ctgttagaaa
    48181 attggggtgc aacctgtatc acacccgatg aaagattaat tagtcaagat tatgatatct
    48241 ttttaacgga taatccgtct aatcttactg cctctggctt gcttttaagc gatgatgagt
    48301 ctggcgtacg agaaattggg cctggtcaat tgtgcgtcaa cttcaatatg agcaacgcta
    48361 tgcaggaagc ggtcttacaa ttaattgaag tgcaactggc acaggaagag gtgacagaat
    48421 cgcctctggg cggagatgaa aatgcgcaac tccatgccag cggctattat gcgctctttg
    48481 tagacacagt accggatgat gttaagaggc tgtatactga agcagcaacc agtgactttg
    48541 ctgcgttagc ccaaacggct catcgtctta aaggcgtatt tgccatgcta aatctggtac
    48601 ccggcaagca gttatgtgaa acgctggaac atctgattcg tgagaaggat gttccaggaa
    48661 tagaaaaata catcagcgac attgacagtt atgtcaagag cttgctgtag caaggtagcc
    48721 tattacatga acaatatgaa cgtaattatt gccgatgacc atccgatagt cttgttcggt
    48781 attcgcaaat cacttgagca aattgagtgg gtgaatgttg tcggcgaatt tgaagactct
    48841 acagcactga tcaacaacct gccgaaactg gatgcgcatg tgttgattac cgatctctcc
    48901 atgcctggcg ataagtacgg cgatggcatt accttaatca agtacatcaa gcgccatttc
    48961 ccaagcctgt cgatcattgt tctgactatg aacaacaacc cggcgattct tagtgcggta
    49021 ttggatctgg atatcgaagg gatcgtgctg aaacaaggtg caccgaccga tctgccgaaa
    49081 gctctcgccg cgctgcagaa agggaagaaa tttaccccgg aaagcgtttc tcgcctgttg
    49141 gaaaaaatca gtgctggtgg ttacggtgac aagcgtctct cgccaaaaga gagtgaagtt
    49201 ctgcgcctgt ttgcggaagg cttcctagtg accgagatcg ctaaaaagct gaaccgcagt
    49261 attaaaacca tcagtagcca gaagaaatct gcgatgatga agctgggcgt cgagaacgat
    49321 atcgccctgc tgaattatct ctcttctgtg accttaagtc cggcagataa agactaatca
    49381 tctgtaggcc agataagacg cgttagtgtc ttatctggca tttgcaccga ttgccggatg
    49441 cggcgtaaac gccttatccg gcctacgatt cccattattt caacagatta cattaaagta
    49501 ggccagataa gacgcgtcag tgtcttatct ggcatttgca ctgaatgccg gatgcggcgt
    49561 aaacgcctta tccgtcctac gaatcccgcg atttcctgac cctctcggca tataccgtca
    49621 gcgtctgttt tatcacatcc agcgttaccg gcttcgagag gcagctgtcc ataccggact
    49681 ccagacaccg ctgcttctct tcagccaacg cattagcagt tactccgatt accggcaacg
    49741 tcagtcccaa ctgacgaatg cgttgcgtca agcggtaacc atccatattt ggcatgttga
    49801 cgtcgctaag cacgatatca atatgattct tactaagtac attaagcgca tcgacgccat
    49861 cattcgcggt tttacattga tagcccaacg atcccaactg atctgccagc agacgtcggt
    49921 taatcggatg atcatccacg accagaatca tcatatcgtc gttatcgctg accgctttgt
    49981 ccgtcgacgg cagagcgtta gcaggatcgt cgctctccat ctcgatcaaa taaatacgcg
    50041 ccaacaatgc cggtagctca tgcggagcag ccacactgtg tacccactcc cctggcgctt
    50101 tctccagcgg aataccaata tggcgacgac agaaggtcac taccgctctg ccctgccatt
    50161 ttttactcac tacctcgtca gtgattaaca catcttcggg agtcggttcc tgcccttcgt
    50221 atgttgtaac gacgatgccg ctgcgctgca aactggtttc caggaactga cagagcgacg
    50281 cattgcggac cgccagccag cagcgtttac cactcaaccc ttccacgcct tttttctgcg
    50341 ggtactgagc gccgtacaac ggaatacgca cggtaaactg gctgcccatt cccggttctg
    50401 aatctaccga gatatcgccg tccatcatgc tgatcagttt ttcacaaatc gccagaccca
    50461 gaccggtccc ctggaagttt cgctgtacgc ccgttccaac ctggaagaag ggatcaaaca
    50521 agcgcaccac ttctttcgcc ggaatcccca cgccggtatc gcgaacacgg atagagagat
    50581 aatcgccatc agcgcgaaca tgcaaaacta tacagccggt atcggtgaat tttatggcgt
    50641 tactcaacag gttggagatg acctgctgta aacgcatcgg gtcgccattt aaggccactg
    50701 gcacatccgg ttcaataaag cagtacaagc ctaactgctt gcgtaccacc agcggtaaat
    50761 agttggcggt gatgtggttc atcacttcac gcggtgaaaa ctcacgcggt tcgatcttca
    50821 actgttccga ttcaatcttc gagaaatcga gaatatcgct gataattttc aacaacaggc
    50881 tggaagagtt gttcattgcc gtcaccagac gatcgacgcc tttcggtaac tctttggttt
    50941 gcaacagatc caggttaccg ataatgccat acagcggcgt tcgcagctca tgactgacgg
    51001 tggcaaggaa catcgatttt gactggctcg cctgttccgc tgcttgtgcc atctcctgca
    51061 acgactcttc catcttcacg cgcgaagaaa catccaccag cacacaaatg gccacgtttt
    51121 cattacgata gcgcgaatgg acgaagctga tttgcagatt ggtattgttg ctggtcagga
    51181 catcaacaaa attgacctgc tgcccacaga taatttgcgt cagtcgttgg cggtcctcat
    51241 gcgtaagcat attgagatag gtatgcgcca gttcgttact taaaatattg acgccatcag
    51301 cggtacgcaa aatgcagata cccactggcg cggaggcgac aatcttgcga ttgaactgct
    51361 catgttcttc cagtcgcagg gcgtcgcttt ccgccggaat gaaaatacgt cgctcgtaca
    51421 tccgcgcgag agtaaacaat gcagctccgg caagcacatt cagcaaaatt gcgttaagga
    51481 tcaacatgcg aatgcgttcc agcaccttat caaccggcac cgaatacacg atgcttagcg
    51541 atgagggtgg cagatttttc ttcagcacca gctcccggaa cccttccgta tagccaaacc
    51601 aggagcgttc ctgcatccag cgaggatcgc ccttaatttt actttctggt ccggtaagcg
    51661 aaatcagggt atgaccattt tcatcaagaa tggtaacccc catcggcaac gtacccggta
    51721 agaaaaagtt ctccatccgg atggtctgct cgacacccaa aagcgcctgc aaccggttcg
    51781 ccagataaac tggcgtcaac gcgtaaaaat acccgacgca agggcgcgga ccttcgctga
    51841 tccagtagag gttactgccg ctatcatctt gtggtgcatt tcgatattta ttgatgcgtt
    51901 catgcaacgc tttcaacgcg gtatcgcgtt ccactggcat atcacgcaga ccgaaattgg
    51961 ccatgcagag gttatcactg ccgattaaaa atacccggtt gagatcgtaa gccgcagaaa
    52021 aattatcgcg ccagtagcgc ataaaccacg ccaatgactc cagagaacct cgccaggtgt
    52081 tactcattgc ggaacaatcg gagtcggcaa acagcggttc aaacgcaggc acatccgcct
    52141 gcgtttctcg tccacgcggg gaaagcacac cgttttctgc cgataagcga ttttcggcga
    52201 tgtacttaag ctctttcatc acatcagagg tgcgttgaat aaagcgctga gcctgatcgg
    52261 aactcagatt aaattcctga cgaatttccg attctcgctg atgtaacgca ttaacgatgt
    52321 aaaaaacgga tgaaaaagca atcaacagcc agagcactaa cgccaatgct ctgaacatgt
    52381 agcgcgaggc tttcagggtt gtacgaaaag aagcaaggta tttcaaaggg gagaagctcc
    52441 gcctcaggtg accgatggag tgtggttaag gtagcggtaa aagcgtgtta ccgcaatgtt
    52501 ctgtcaccat tgcaataatg cgtatcgctt agaaaccatt gccttaacca ggaaagccgg
    52561 agtagatgga cagttctcag tacgaaaaat aagaaatgac caatctgccg acgcttgtta
    52621 atggttatat cctggcgtcg gcagatcgcc cagcagtctt atctgaaaaa agagagagcc
    52681 aggttgtgtg aagggatgga tcaccagtca aaacttttca ccagcgtcag ctcgccagca
    52741 ttacgcatcg gtacaataaa tgtttcctgt ttctcattga ccgatccttc atcggtgatc
    52801 agcgtcagtt gggcggtcgt caattccgtt tcgctgcgcc caccatagta gttgatatac
    52861 acctgatagc gcccgtgaac tggtgctggc atggcgaaaa tctcgggtcc gtaccccgtc
    52921 gtgacatcca tatccagtgc accactgttt ttcagcacgg tgttaccgta ccaggcgtgt
    52981 tcgccatcgg gcgtgacaac atggagatcg agatcggtat tgtccgtatc ccacgagaga
    53041 accagccgta aacgtgcacg aatagttcct gtacccggcg ttgagtaaaa ctgcattttt
    53101 tgccggcttt gcccatccgg gctgatgacc tgcacgctgt tgctgccttc agtgaaaata
    53161 taagggcgtg caaatgaacc atcggattca attctctgcg gcatactggc accgttgacc
    53221 accagccggc cctgctgaac tttacccgcc gcagcataat tctttatttt gccgcggatc
    53281 tgcgctgaga tattttgatc atctgccatg ttgacggacg acgccgggta attgatcgat
    53341 tgtgaaaaac tggcatcttc gccctctgcc ggatgccagc cagagagcgg tgcgtcaatc
    53401 tcgacttcct gcacaccttc actgtgagcg acaggcgaaa gtgccaccag taacaacgga
    53461 agaaaaatct ttcgcatagc attagtccat taataattgt cgggtcagcg tttcgatata
    53521 gttctcatca agcccggtgg gataaccatc aaaggccaag tgcagatact catgggttaa
    53581 atccagccga tcctgaagcg taaagaagtt acgaataaac agccgtttct gctgccgatc
    53641 ggtatagggg aaacctgaga ctaaacgaca gaccgcaaac acgtctggtt cgttgtaccc
    53701 cgtctcgcct tgtaatatac gccgccactg cggcattttt ttcgccagcc aggctttcgc
    53761 tttgggtaat aactggcagg ttgagcgtgg agctccccag cggctaaggc tgttgtcggg
    53821 ataagcaaac gcgagtatct ggtcgtaacg ctccccctgc cccgcctggg ccgtggcttg
    53881 ccgccaggaa agtgtccctt cggtagcgcg actgccatga taatgaaccg gatcgcctgc
    53941 atagatgagc tcctgcgtcc aggcggtcat cgttcgcgcc cccgtggtcg ctggcgaagc
    54001 agaaacacgc tgcgtggcgc tgctgtcggg gatggtcagg caatcacctt cccgattggc
    54061 gttttgctgt aaaaaagtac gtatcgccac agtcatggct ttcgctgcct caggtggcgt
    54121 acttttcgcc tcgcgatcca gaactctggt aacgtactct tcacgatcga gatgcgactg
    54181 caatttcagt ttgccctttt cgctcagtaa ggtcgtctca ccgtggctga caaacgtaat
    54241 gtggttacca ttagtaaacg tcacacgata gcgcccatta agtacgctgg gttttactgc
    54301 cgtcgtactc ttttccgctg tgattttctt cagcggatag cgggcaaaca gctccacctc
    54361 tacacattgc ccactggcaa cctgcgtcgg cactggcaaa acccgattaa gcaccgtcgc
    54421 ataacgggtt agtaccgttt tgctggtgcc actgccagta acccacagcg gtgtaccatc
    54481 ggttaaccag ccagcaaaac caccctgacg gatttcctgt ttatcatcgg caaaccagct
    54541 ccaggttttc acccgtaccc gactaccgag catggatgcg acaccgattt tcgcctcgtc
    54601 cagcaccaca tccagtaaga cttcctgagc tttgttttgc gcaggtaacg tcgctaatga
    54661 atcaagcagc gatttaaccg ttaccgaggt ttctggcttc agcgttgtaa gagacgctaa
    54721 ccacgcaggt gcctgacgct gttgccagta ttgcccccac acatctgcgc caatatgcaa
    54781 ccgctgcggc gcaaaataga gcccacagga acgtaccagc gcggtatcgc gcgttatgct
    54841 ctccccagcc tgacaacaat aaacctcttc cggggagttt ccgcgacact ggtagggctg
    54901 ctccggttga tgggtatcct ccagccaggc gtaaacatac agtttccaca aactccctaa
    54961 cggcgtggtg agcgagtcag gtaatgctga gactttggtc acgcctgatg acgacagctg
    55021 atacagttga tcgtgctgcg caccgcgcag caccagttgt aacggcgtct cctccgcaag
    55081 cgttggcaga gtaaccaacg ccaatagcca gacgattctg cgccagttca cttatttcac
    55141 ctgcatcccg gtccattcac tccctgccgc gacactttgc tgcgcaggtg cataggaacg
    55201 cacataacga gcaggaggca gaacgaattg ccctttttgc gagaagcgca gcaaatggcg
    55261 gaaagtgacc gttcccgtca gttctttcac cggcaccata taggccagtt cgcccatttc
    55321 attacgcgct ttttccagca attgcccctg ctgtttcgcg gcgttgggtt tattgactga
    55381 aatgccccat gttgtgcgct caacgtcggc tcccggcggg agcggtactt ccacctgacc
    55441 atagcgcaga actgcatcct gctcgctggt aagcgtaatt tcatcgagat acagcgcatc
    55501 gctgtcaatc tcattgctgg tcaccggctg cagagtaaag ctcatctctt cttcaccggg
    55561 gataagccga tacaactggc gttcaacggt caccggaatg ttactttgtt gagccgtttt
    55621 tgccggttca cgccagcgga cctgcacatt tgcggcgata attcgtcacc aaaagagaga
    55681 atgtccggca caccctaacc aacccaacgc cagtcttcgc cccctccagt taacttatgt
    55741 ttagcccatg cgcccgcagg cgcaggcaaa acaactggag gcattgtcgc catatatttc
    55801 gccagccagt tcatggccag cgcgcgctca atagtggatt gctcagcggt caaaccatta
    55861 aaatagcggc cgtctgggta gcgtcaccac cgctattgag cagcacaacc gtgtgcgcca
    55921 gcggctgatt gctgtttgcc gctttatccc acgcatattg ctgaacctgt ggcatcaccg
    55981 tggacttcaa ctgcgctttt ttcagcaacg tcatggttaa gacgtttgcc accgcatctg
    56041 ccagtggaga ttccggtgta tcgaggatca ggctgtcatt gatatcgcgg atgtcttcct
    56101 cagagaaatc ttcatcttta gttccgcgcc gggcgatagc ttcatccccc cctttcaaca
    56161 acgttttgca cggcagattc atctcctgtg cccacgccag caccagcgcc cgatgcaata
    56221 acggcatgtt atccgcctgc tcggcataac tgtcgagcat atgctgccag tattccggtt
    56281 gttgcgttac gccgagcgcc tggctggcct gccagtcggc gtaccatgcc catgccgtaa
    56341 ggaaggcgtc accattgcca tcttcacccc accaggtaaa gcgcgctccg ggccccgcca
    56401 gttgcatcag ccgcagacgg ttatcctgaa tcatctgacg aatgtcgtta gcggcggcac
    56461 tttgatgatc ggcaagcgaa cgccaggcga gactgagcgg gatcagacgg ctaccggtgt
    56521 tgatcacccc accccacggt tcatcgacta acgcatcaag attgttgcgg aaaatctcct
    56581 gcggcgtttc actactttgt agccggatat tactcgcctg ctcgggcaac atcagcgcgt
    56641 tctcgccacc gccgagcatg acatttttct gctgttcaac gggccagctg ttgtccacaa
    56701 aagacagttt tgtgctaatg ctgtcctgca cttgcccatt ttgttgcagt tctgcactta
    56761 acaagccaga ttgctgaata ttctgcgtca gcgaaatata attcgcccct ttgtgcagcg
    56821 tcagcgtctg gcgcatctca gcgcctgcaa atttagtcac cagcgccacc ggttcgttat
    56881 cctgctgact gaagataaac agtcctgccg ccggtttgtc gcccacgcga tacaccgttg
    56941 gcatactcca cttcatgtag agattttttt ccgaacgcag ataagcacgc ccctgcccga
    57001 ccagcccgtc gccgttcatc ccacgcgcgg tgatacgcca gcgggttaac gaatcaggca
    57061 tcaggaacgt gaaatacgct ttgccttgtt tatcggttgt gagtgacggc atccatgccg
    57121 cggtatccac ctcttcacgc cgtggacgtt caagcatttt tactcgccgc tcgctgcggt
    57181 tagtcgcgcc aggcgcaacc ggctcgctgg agagcgcctg gtcatagctg ataaacgaca
    57241 gactggagct ggtacgcaca ttgttacgcc ccagcggata gaaaaatttg ccgatattcg
    57301 gcgcgatttc tggttgcagc gcgtagatca tttcatcgac cacgcctacc gttagctgcg
    57361 cagaaacagg tttacctttc agcgacgagg ttaattctga agtggtcaac aaaaactggc
    57421 caccgagtta gagtttttcc agtatcgatt ttccga
//
LOCUS       DAJVPP010000010        55420 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVPP010000010 DAJVPP010000000
VERSION     DAJVPP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.010"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(103..1476)
                     /gene="argH"
                     /locus_tag="OQM34_000632"
     CDS             complement(103..1476)
                     /gene="argH"
                     /locus_tag="OQM34_000632"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1451199.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1537..2313)
                     /gene="argB"
                     /locus_tag="OQM34_000633"
     CDS             complement(1537..2313)
                     /gene="argB"
                     /locus_tag="OQM34_000633"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1451200.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2321..3325)
                     /gene="argC"
                     /locus_tag="OQM34_000634"
     CDS             complement(2321..3325)
                     /gene="argC"
                     /locus_tag="OQM34_000634"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1451201.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3479..4630
                     /gene="argE"
                     /locus_tag="OQM34_000635"
     CDS             3479..4630
                     /gene="argE"
                     /locus_tag="OQM34_000635"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1451202.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4982..7633
                     /gene="ppc"
                     /locus_tag="OQM34_000636"
     CDS             4982..7633
                     /gene="ppc"
                     /locus_tag="OQM34_000636"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1451203.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7816..9549
                     /locus_tag="OQM34_000637"
     CDS             7816..9549
                     /locus_tag="OQM34_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1451204.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9698..10549
                     /locus_tag="OQM34_000638"
     CDS             9698..10549
                     /locus_tag="OQM34_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1451205.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10536..10877)
                     /locus_tag="OQM34_000639"
     CDS             complement(10536..10877)
                     /locus_tag="OQM34_000639"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1451206.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10879..11757)
                     /locus_tag="OQM34_000640"
     CDS             complement(10879..11757)
                     /locus_tag="OQM34_000640"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1451207.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11723..14020)
                     /locus_tag="OQM34_000641"
     CDS             complement(11723..14020)
                     /locus_tag="OQM34_000641"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1451208.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14071..14391)
                     /locus_tag="OQM34_000642"
     CDS             complement(14071..14391)
                     /locus_tag="OQM34_000642"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1451209.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14406..15485)
                     /locus_tag="OQM34_000643"
     CDS             complement(14406..15485)
                     /locus_tag="OQM34_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1451210.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15794..16897
                     /locus_tag="OQM34_000644"
     CDS             15794..16897
                     /locus_tag="OQM34_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS1451211.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16898..18295
                     /gene="ptsP"
                     /locus_tag="OQM34_000645"
     CDS             16898..18295
                     /gene="ptsP"
                     /locus_tag="OQM34_000645"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1451212.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18307..18969
                     /gene="fsa"
                     /locus_tag="OQM34_000646"
                     /pseudo
     CDS             18307..18969
                     /gene="fsa"
                     /locus_tag="OQM34_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18980..20081
                     /gene="gldA"
                     /locus_tag="OQM34_000647"
                     /pseudo
     CDS             18980..20081
                     /gene="gldA"
                     /locus_tag="OQM34_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20356..20973
                     /locus_tag="OQM34_000648"
     CDS             20356..20973
                     /locus_tag="OQM34_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS1451213.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSSHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OQM34_000649"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OQM34_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1451214.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OQM34_000650"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OQM34_000650"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1451215.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPPSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OQM34_000651"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OQM34_000651"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1451216.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OQM34_000652"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OQM34_000652"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1451217.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OQM34_000653"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OQM34_000653"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1451218.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OQM34_000654"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OQM34_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1451219.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OQM34_000655"
     CDS             30120..30728
                     /locus_tag="OQM34_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1451220.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OQM34_000656"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OQM34_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1451221.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OQM34_000657"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OQM34_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1451222.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OQM34_000658"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OQM34_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1451223.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OQM34_000659"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OQM34_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1451224.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OQM34_000660"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OQM34_000660"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1451225.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OQM34_000661"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OQM34_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1451226.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OQM34_000662"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OQM34_000662"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1451227.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OQM34_000663"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OQM34_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1451228.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OQM34_000664"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OQM34_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1451229.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OQM34_000665"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OQM34_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OQM34_000666"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OQM34_000666"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1451230.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OQM34_000667"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OQM34_000667"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1451231.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OQM34_000668"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OQM34_000668"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1451232.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OQM34_000669"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OQM34_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1451233.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OQM34_000670"
     CDS             complement(44843..45142)
                     /locus_tag="OQM34_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1451234.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OQM34_000671"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OQM34_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OQM34_000672"
     CDS             45895..46494
                     /locus_tag="OQM34_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1451235.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OQM34_000673"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OQM34_000673"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1451236.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OQM34_000674"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OQM34_000674"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1451237.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OQM34_000675"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OQM34_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1451238.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OQM34_000676"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OQM34_000676"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1451239.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OQM34_000677"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OQM34_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1451240.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OQM34_000678"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OQM34_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1451241.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OQM34_000679"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OQM34_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1451242.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OQM34_000680"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OQM34_000680"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1451243.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OQM34_000681"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OQM34_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1451244.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13496 a        15019 c        14164 g        12741 t
ORIGIN      
        1 aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg cactttgtag
       61 gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta accgagcctg
      121 cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg ccgcacgctt
      181 ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca cctgactgaa
      241 tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa tggcttccac
      301 caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg ccaccaggta
      361 atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac aacgcggacg
      421 tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca gccaggtatc
      481 gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag ccagcggcaa
      541 acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc atttaccgcg
      601 aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac cggaagtcac
      661 gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca gatcttcagc
      721 aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca acacatggtc
      781 acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca gccagcctgc
      841 taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc ctaatgggct
      901 gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca gcatctcaac
      961 ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt gagtgtaacc
     1021 tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt gcagctgccg
     1081 gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt cagtcgctac
     1141 ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca ctttgtcgat
     1201 cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga tttgttgtgg
     1261 cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg cctgctcttc
     1321 tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc caacaatatc
     1381 ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga accgttgatc
     1441 tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt attttgaaat
     1501 tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc gcgtacccat
     1561 cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag cgatatctac
     1621 cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta tcatgccgtc
     1681 agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt cggcaatgcg
     1741 ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat ccgcgcccag
     1801 cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt gcccttcgtc
     1861 tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg agttgataag
     1921 cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa gctgggtcac
     1981 tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat gtttcttcgc
     2041 ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa tgtctatctg
     2101 atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca gccctttcat
     2161 cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct gatgtgactc
     2221 acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat ccagcagtac
     2281 gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga gactgcgttt
     2341 ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg cctttcagta
     2401 agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac ccgatatcgc
     2461 aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac agccgcacca
     2521 gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg gtcacacccg
     2581 atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc agatgtgggg
     2641 tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga tgagtaaaga
     2701 cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc ttacgtcctg
     2761 caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca agaagatcgg
     2821 catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct ggcaccgcaa
     2881 tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag gctgcctgtt
     2941 ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag gtggcgtcgt
     3001 taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga aactgcggcg
     3061 ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc cctgggctaa
     3121 actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt agctgcggat
     3181 gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc aaagcggtta
     3241 tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg tagccgctgg
     3301 cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct ggttgccagg
     3361 ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac gttagtgtat
     3421 ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg tgtcaacaat
     3481 gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca caccttcaat
     3541 aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc tgctggcgga
     3601 ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa ctcgcaacaa
     3661 atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg cggggcatac
     3721 cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac tgacggagca
     3781 tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg cgtttatcct
     3841 tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca ttctggcgac
     3901 tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta ccgccctgcg
     3961 cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg cacataaagg
     4021 tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg atccagcacg
     4081 cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc aattgcgcga
     4141 taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta cgctcaacct
     4201 cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg agttgcatat
     4261 ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc tcaacgatgc
     4321 attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc tgcatccgcc
     4381 gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg agaaattgct
     4441 cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc aaacgttatg
     4501 cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg atgaatatct
     4561 ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc accatttttg
     4621 ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc gttgccaaac
     4681 tccagtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc tacacctttg
     4741 gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa agcgtcgtga
     4801 atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg cagcatttga
     4861 cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg agcatattcg
     4921 cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg tctggggtaa
     4981 tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca aagtgctggg
     5041 agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa ctatccgtaa
     5101 gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgt caggagttgc tcaccacctt
     5161 acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc agttcctgaa
     5221 cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag ctgccagcaa
     5281 cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac tgagcgaaga
     5341 caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg ctcacccaac
     5401 cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct gtttaaaaca
     5461 gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc gcctgcgcca
     5521 gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa gcccggtaga
     5581 tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg taccgaatta
     5641 cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc ccgtcgaatt
     5701 tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc cgaacgtcac
     5761 tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg atctgttcct
     5821 gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc ctgaactgct
     5881 ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga aaaacctgcg
     5941 ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg aagaactgcc
     6001 aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct acgcttgcta
     6061 ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc tcgacaccct
     6121 gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc aggagagcac
     6181 ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg actacgaaag
     6241 ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca aacgtccgct
     6301 tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata cctgccaggt
     6361 gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg cgaaaacgcc
     6421 gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt ttgcgatgcc
     6481 ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg tcatgaccca
     6541 gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg tgatgattgg
     6601 ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc aatatcaggc
     6661 acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt tgttccacgg
     6721 tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc tgtcacaacc
     6781 gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga tccgctttaa
     6841 atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg cgattctgga
     6901 agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta tggatgaact
     6961 gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag attttgtgcc
     7021 ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg gttcacgtcc
     7081 ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt ggatttttgc
     7141 ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg cgctgcaaaa
     7201 agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt ggccattctt
     7261 ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt ggttggcgga
     7321 atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag agttacgcaa
     7381 cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc atctgatggc
     7441 cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg acccgctgaa
     7501 cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag gccaggaacc
     7561 ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg caggtatgcg
     7621 taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag ggttttctga
     7681 aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt tattcaaatt
     7741 ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat atttttttca
     7801 agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc tagctggaaa
     7861 gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca ggccattatt
     7921 tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt cagctcgctg
     7981 tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc agtgatcggc
     8041 gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta cgggcaggag
     8101 ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc cagcgagtat
     8161 ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac ggcggtggca
     8221 gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg ttatgttgtc
     8281 tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac gtttatcaaa
     8341 aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc tgccgcaccg
     8401 tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct aacaaagtta
     8461 ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc gggtaacgaa
     8521 ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat gagtctgtac
     8581 ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga tccgaatctg
     8641 accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct gcaacaggcg
     8701 ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc gctgatgaac
     8761 atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac gatgaccgcc
     8821 cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta catgaaccag
     8881 caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt ccaggatgtg
     8941 ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac gcatatcaaa
     9001 tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga tcatgttccg
     9061 ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc taacctgtat
     9121 aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc gaacggattc
     9181 ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca caaaactcag
     9241 gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct gctgtggacg
     9301 tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga tcgtaaatac
     9361 agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta cgacggttac
     9421 gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg gattggtaac
     9481 ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga tcaggtgggt
     9541 aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga tactgccgca
     9601 atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt aataatgcca
     9661 gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg tcagctatct
     9721 gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg ccagcagtaa
     9781 cggcccagtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa ttgtgctgga
     9841 aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg tgctgtacgt
     9901 tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct ttagcgtgct
     9961 gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac aatatcaaaa
    10021 tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc tgctacaaac
    10081 gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta tcgtagccag
    10141 cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct cacgcagcca
    10201 ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg cgcttacccg
    10261 cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc tgtttcaaaa
    10321 aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc acgctaagac
    10381 gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg gctttgtcga
    10441 cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt cggaatatcg
    10501 ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag atgggtttgc
    10561 tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc acgcaggaat
    10621 gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc ggcaagctcg
    10681 atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc taaacgattc
    10741 tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa ctggcacaac
    10801 ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca ggcggttact
    10861 gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc gttctgccat
    10921 ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca tcgaccatgt
    10981 tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca gatggatctg
    11041 cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc ggctgagcgt
    11101 gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca ccagcaaacg
    11161 cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct gagtcgcgtc
    11221 cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta acagcttgct
    11281 tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc gcagtcgctg
    11341 taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa gcgtgacgcc
    11401 gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca gagcgtcaag
    11461 gctgatatcg cgaccaatcc gttcaaacgc cccagagggg cattcatccg catcacgcaa
    11521 acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt tgccggagat
    11581 cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa agaccaccgt
    11641 acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc gcgccacatc
    11701 atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg atgtcatcct
    11761 ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc accaccagcc
    11821 cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc accacgttaa
    11881 actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc agtttgcgta
    11941 atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac gtaccgttag
    12001 acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc ccttgtgcgt
    12061 cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca aaacgaccgt
    12121 ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta tacgatcccg
    12181 gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag tagtggcgca
    12241 acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc tcaaagcggt
    12301 taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct tttaataccg
    12361 acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc aacgcatgga
    12421 gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta taacgcgcgc
    12481 cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac gagagcagcg
    12541 gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca accatcaggg
    12601 tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa gtcagcgcag
    12661 cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta aacatcgcga
    12721 tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc actacggaat
    12781 agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc accacttcat
    12841 cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt ttcatcagga
    12901 acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt aattgcacgc
    12961 tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt ccgttctcgg
    13021 ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa cgcgcgctac
    13081 tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg agcagttctt
    13141 tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc aacatatact
    13201 ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga atgatgttca
    13261 tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc gcgttatggc
    13321 gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg cagtttgccg
    13381 ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct tccagcagta
    13441 acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc tgcatctgtg
    13501 ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata atgtgcccct
    13561 ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct tttacttcat
    13621 ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac gggaacaact
    13681 cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc ggacgcgtcg
    13741 ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac ataatcccgg
    13801 cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc gaaatttcaa
    13861 catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt tcgccttcgg
    13921 tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca cgggtattgg
    13981 caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc tgtgggaaag
    14041 cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt gagcttcaat
    14101 tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc gcacgatggt
    14161 cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa tcaccatatc
    14221 agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc cctgggtttc
    14281 tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg ctgccatata
    14341 ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca tggctcgttc
    14401 ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc agtcgaagac
    14461 ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac cactgctccg
    14521 acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac aatcagacca
    14581 ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc acaaactgaa
    14641 ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg aatcgcccct
    14701 tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg ctcttctgcg
    14761 gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg gatacaaata
    14821 cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat cagcatgaag
    14881 gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag ccccatgatc
    14941 accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt caggctgttg
    15001 gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt aatcagtgtg
    15061 ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg caccggaatt
    15121 ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc accaaagaag
    15181 cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag cgcagaacgc
    15241 tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt caaacctgca
    15301 acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg cactgcgcct
    15361 ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac gaagggaatc
    15421 atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg caccaactca
    15481 ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg gaggaaacaa
    15541 taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga cttttgtaat
    15601 atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca tgccgcaaaa
    15661 cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc agcttttggc
    15721 agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag aaaaccgtac
    15781 cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta acggcgtaca
    15841 tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc aaattgagtg
    15901 gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc tgattggcac
    15961 cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg aacaggaagg
    16021 gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg cgccgctggc
    16081 ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc tgaatccgca
    16141 aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga cgccgatctc
    16201 ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg acgccgagca
    16261 atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc gtctgctgga
    16321 tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg gcgatacttc
    16381 cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg aagcgattgt
    16441 cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat acctgcaaga
    16501 acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct acggtgagca
    16561 acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg atgaactgac
    16621 ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac tgaaaagcgg
    16681 cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga cgctggttgg
    16741 tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg acggcaacgc
    16801 cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc aagaagcccg
    16861 cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag cccgtaccgc
    16921 tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag cgcaggccgc
    16981 attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct atatggatcg
    17041 caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc tggagtctgc
    17101 caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac ccgttgatta
    17161 tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc gtatttatga
    17221 agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct ccgctcacgg
    17281 cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat gggtgaaaga
    17341 aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg atgagaagat
    17401 ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc aatgctgcga
    17461 agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc tggcagtgga
    17521 tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat tcttgcgggc
    17581 gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc tgtgcggtga
    17641 gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg atgaactcag
    17701 catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg atagccgtga
    17761 gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag tggaacacct
    17821 gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg agtgcatcac
    17881 gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg ataacctgct
    17941 gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg cgcgcgaggc
    18001 cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat cagaacacat
    18061 tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg gcgatgatga
    18121 agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc agcacatgcg
    18181 cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg cgctggttaa
    18241 cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac tgtaaaagga
    18301 aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg tctggcacgc
    18361 atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag caaggagtcc
    18421 atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat tctgtttgct
    18481 cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct gcgcgacgct
    18541 attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc aattaaaata
    18601 ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc agaagggtta
    18661 ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt agatgcccag
    18721 ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat gcacgcgcca
    18781 gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga ctgtttactg
    18841 gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct caacacccct
    18901 gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt tggcactact
    18961 catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat acatccaggg
    19021 cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac gctggttagt
    19081 ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa gctttaaaga
    19141 tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa atgagatcga
    19201 ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta tcggtggcgg
    19261 aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg tagcgatcgc
    19321 accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct acaccgatga
    19381 gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca ttgtcgacac
    19441 caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg atgcgctggc
    19501 aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg cgggcggcaa
    19561 gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc tggaagaagg
    19621 cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg agcgcgtgat
    19681 tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc ggcggctcac
    19741 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaag
    19801 tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag gaaatcgaaa
    19861 ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa ctggttatta
    19921 aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca gaaggtgaaa
    19981 ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca ctgctggtag
    20041 ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca aactcccggc
    20101 ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg cctacgcaat
    20161 ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc actccgcatc
    20221 cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt tatccgcttc
    20281 tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg actcttattt
    20341 tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac ggcatttacc
    20401 agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc
    20461 aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt ttacgatccg
    20521 gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagcagcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg ggtcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaggcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattagcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccggaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAJVPP010000011        54313 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVPP010000011 DAJVPP010000000
VERSION     DAJVPP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.011"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..238)
                     /locus_tag="OQM34_000682"
     CDS             complement(<1..238)
                     /locus_tag="OQM34_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1451245.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANV"
     gene            complement(235..1161)
                     /locus_tag="OQM34_000683"
     CDS             complement(235..1161)
                     /locus_tag="OQM34_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1451246.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            1270..1932
                     /gene="can"
                     /locus_tag="OQM34_000684"
     CDS             1270..1932
                     /gene="can"
                     /locus_tag="OQM34_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1451247.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(1973..2509)
                     /gene="hpt"
                     /locus_tag="OQM34_000685"
     CDS             complement(1973..2509)
                     /gene="hpt"
                     /locus_tag="OQM34_000685"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1451248.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            2715..5105
                     /gene="gcd"
                     /locus_tag="OQM34_000686"
     CDS             2715..5105
                     /gene="gcd"
                     /locus_tag="OQM34_000686"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1451249.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(5152..6702)
                     /gene="cueO"
                     /locus_tag="OQM34_000687"
     CDS             complement(5152..6702)
                     /gene="cueO"
                     /locus_tag="OQM34_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS1451250.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKRPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            6868..7215
                     /locus_tag="OQM34_000688"
     CDS             6868..7215
                     /locus_tag="OQM34_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1451251.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            7321..8187
                     /gene="speE"
                     /locus_tag="OQM34_000689"
     CDS             7321..8187
                     /gene="speE"
                     /locus_tag="OQM34_000689"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1451252.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            8203..8997
                     /gene="speD"
                     /locus_tag="OQM34_000690"
     CDS             8203..8997
                     /gene="speD"
                     /locus_tag="OQM34_000690"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1451253.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(9035..9397)
                     /locus_tag="OQM34_000691"
     CDS             complement(9035..9397)
                     /locus_tag="OQM34_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1451254.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(9572..12169)
                     /gene="acnB"
                     /locus_tag="OQM34_000692"
     CDS             complement(9572..12169)
                     /gene="acnB"
                     /locus_tag="OQM34_000692"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1451255.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            12524..14377
                     /locus_tag="OQM34_000693"
                     /pseudo
     CDS             12524..14377
                     /locus_tag="OQM34_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(14448..15872)
                     /gene="lpdA"
                     /locus_tag="OQM34_000694"
     CDS             complement(14448..15872)
                     /gene="lpdA"
                     /locus_tag="OQM34_000694"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1451256.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(16197..18077)
                     /gene="aceF"
                     /locus_tag="OQM34_000695"
     CDS             complement(16197..18077)
                     /gene="aceF"
                     /locus_tag="OQM34_000695"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1451257.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(18092..20755)
                     /gene="aceE"
                     /locus_tag="OQM34_000696"
     CDS             complement(18092..20755)
                     /gene="aceE"
                     /locus_tag="OQM34_000696"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1451258.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(20916..21680)
                     /gene="pdhR"
                     /locus_tag="OQM34_000697"
     CDS             complement(20916..21680)
                     /gene="pdhR"
                     /locus_tag="OQM34_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1451259.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            22221..23594
                     /gene="aroP"
                     /locus_tag="OQM34_000698"
     CDS             22221..23594
                     /gene="aroP"
                     /locus_tag="OQM34_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1451260.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(23637..24491)
                     /gene="ampE"
                     /locus_tag="OQM34_000699"
     CDS             complement(23637..24491)
                     /gene="ampE"
                     /locus_tag="OQM34_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1451261.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(24488..25039)
                     /gene="ampD"
                     /locus_tag="OQM34_000700"
     CDS             complement(24488..25039)
                     /gene="ampD"
                     /locus_tag="OQM34_000700"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1451262.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            25127..26020
                     /gene="nadC"
                     /locus_tag="OQM34_000701"
     CDS             25127..26020
                     /gene="nadC"
                     /locus_tag="OQM34_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1451263.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            26223..26663
                     /gene="ppdD"
                     /locus_tag="OQM34_000702"
     CDS             26223..26663
                     /gene="ppdD"
                     /locus_tag="OQM34_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS1451264.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            26673..27820
                     /gene="tadA"
                     /locus_tag="OQM34_000703"
                     /pseudo
     CDS             26673..27820
                     /gene="tadA"
                     /locus_tag="OQM34_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            27810..29012
                     /gene="hofC"
                     /locus_tag="OQM34_000704"
     CDS             27810..29012
                     /gene="hofC"
                     /locus_tag="OQM34_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCS1451265.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIIGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(29047..30090)
                     /gene="guaC"
                     /locus_tag="OQM34_000705"
     CDS             complement(29047..30090)
                     /gene="guaC"
                     /locus_tag="OQM34_000705"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1451266.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(30252..30290)
                     /locus_tag="OQM34_000706"
     CDS             complement(30252..30290)
                     /locus_tag="OQM34_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451267.1"
                     /translation="MIIKTLKMSARK"
     gene            30315..30935
                     /gene="coaE"
                     /locus_tag="OQM34_000707"
     CDS             30315..30935
                     /gene="coaE"
                     /locus_tag="OQM34_000707"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1451268.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            30935..31678
                     /gene="zapD"
                     /locus_tag="OQM34_000708"
     CDS             30935..31678
                     /gene="zapD"
                     /locus_tag="OQM34_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1451269.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            31688..31885
                     /gene="yacG"
                     /locus_tag="OQM34_000709"
     CDS             31688..31885
                     /gene="yacG"
                     /locus_tag="OQM34_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1451270.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(31985..32374)
                     /gene="mutT"
                     /locus_tag="OQM34_000710"
     CDS             complement(31985..32374)
                     /gene="mutT"
                     /locus_tag="OQM34_000710"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1451271.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRNITLWFWLVESWEGVPWGKEGQPG
                     EWMSLIGLNADDFPPANEPVIAKLKRL"
     gene            complement(32434..35139)
                     /gene="secA"
                     /locus_tag="OQM34_000711"
     CDS             complement(32434..35139)
                     /gene="secA"
                     /locus_tag="OQM34_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1451272.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(35201..35713)
                     /gene="secM"
                     /locus_tag="OQM34_000712"
     CDS             complement(35201..35713)
                     /gene="secM"
                     /locus_tag="OQM34_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1451273.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(35944..36861)
                     /gene="lpxC"
                     /locus_tag="OQM34_000713"
     CDS             complement(35944..36861)
                     /gene="lpxC"
                     /locus_tag="OQM34_000713"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1451274.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(36962..38113)
                     /gene="ftsZ"
                     /locus_tag="OQM34_000714"
     CDS             complement(36962..38113)
                     /gene="ftsZ"
                     /locus_tag="OQM34_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1451275.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(38174..39436)
                     /gene="ftsA"
                     /locus_tag="OQM34_000715"
     CDS             complement(38174..39436)
                     /gene="ftsA"
                     /locus_tag="OQM34_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1451276.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(39433..40263)
                     /gene="ftsQ"
                     /locus_tag="OQM34_000716"
     CDS             complement(39433..40263)
                     /gene="ftsQ"
                     /locus_tag="OQM34_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1451277.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(40265..41185)
                     /gene="ddlB"
                     /locus_tag="OQM34_000717"
     CDS             complement(40265..41185)
                     /gene="ddlB"
                     /locus_tag="OQM34_000717"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1451278.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRSEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(41178..42653)
                     /gene="murC"
                     /locus_tag="OQM34_000718"
     CDS             complement(41178..42653)
                     /gene="murC"
                     /locus_tag="OQM34_000718"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1451279.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(42707..43774)
                     /gene="murG"
                     /locus_tag="OQM34_000719"
     CDS             complement(42707..43774)
                     /gene="murG"
                     /locus_tag="OQM34_000719"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1451280.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(43771..45015)
                     /gene="ftsW"
                     /locus_tag="OQM34_000720"
     CDS             complement(43771..45015)
                     /gene="ftsW"
                     /locus_tag="OQM34_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1451281.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(45015..46331)
                     /gene="murD"
                     /locus_tag="OQM34_000721"
     CDS             complement(45015..46331)
                     /gene="murD"
                     /locus_tag="OQM34_000721"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1451282.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(46334..47416)
                     /gene="mraY"
                     /locus_tag="OQM34_000722"
     CDS             complement(46334..47416)
                     /gene="mraY"
                     /locus_tag="OQM34_000722"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1451283.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(47410..48768)
                     /gene="murF"
                     /locus_tag="OQM34_000723"
     CDS             complement(47410..48768)
                     /gene="murF"
                     /locus_tag="OQM34_000723"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1451284.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(48765..50252)
                     /gene="murE"
                     /locus_tag="OQM34_000724"
     CDS             complement(48765..50252)
                     /gene="murE"
                     /locus_tag="OQM34_000724"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1451285.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(50239..52005)
                     /gene="ftsI"
                     /locus_tag="OQM34_000725"
     CDS             complement(50239..52005)
                     /gene="ftsI"
                     /locus_tag="OQM34_000725"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1451286.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(52021..52386)
                     /gene="ftsL"
                     /locus_tag="OQM34_000726"
     CDS             complement(52021..52386)
                     /gene="ftsL"
                     /locus_tag="OQM34_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1451287.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(52383..53324)
                     /gene="rsmH"
                     /locus_tag="OQM34_000727"
     CDS             complement(52383..53324)
                     /gene="rsmH"
                     /locus_tag="OQM34_000727"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1451288.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(53326..53784)
                     /gene="mraZ"
                     /locus_tag="OQM34_000728"
                     /pseudo
     CDS             complement(53326..53784)
                     /gene="mraZ"
                     /locus_tag="OQM34_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
BASE COUNT        12863 a        15141 c        13380 g        12929 t
ORIGIN      
        1 cgacgttagc gtaggcgttg gtgatcaccg acatcatgat cagccccggt acgatgaact
       61 gcatatagct gaagccatgc atatcgccaa tacgcgaacc tatcaggtta ccgaagataa
      121 taaagtaaag ggtcatggtg atgactggcg gcaccagcgt ctgcacccag atacgcataa
      181 agcgatggat ctctttcgcc cagatgcttt ttagcgccac ccagtaaaga tgcatcatgc
      241 gcgatctcct tgtttttcat taaccagtga aacaaacagc tcttccagac ggttagcttt
      301 gttacgcata cttaatacct gaatgccctg ctcacttaac tgcgtaaata cgctgttgat
      361 cccctgctca cgtagcactt caacttccag cgtcgcggta tcgaccagtc gatactgata
      421 gccatcgagc ttcggtaacg gacttttcgg tgcgagatcg agaataaagg tttccgattt
      481 cagcttcgcc agcagcgcct tcatcgaggt attttccacc agctcaccat gttgaataat
      541 gccgatattg cggcacagca tttctgcttc ttccaggtag tgtgtggtaa gaatgatggt
      601 ggtgcctttg tcgtttaaat ccttcaaaaa gccccacatt gagcggcgaa gttcaatatc
      661 cacgcctgcg gtcggttcgt cgagaatcag tagtttaggt tcatgcatta acgcacgggc
      721 aatcattaaa cggcgcttca tcccgccaga taacatacgc gcacgttcgt tgcgttttcc
      781 ccataaatcg agctgcttaa gatatttttc gctgcggatg tacgcctctt tgcgctccac
      841 accgtagtac cctgcctgat tcaccacaat ttgctgcacg gtttcaaacg ggttgaagtt
      901 aaattcctgc ggcaccagtc ccaactgacg tttagcgttc acgacatctt tctcgagatc
      961 gtaaccaaat acgctgaccc gcccggaggt tttatttacc agagagctga taataccgat
     1021 agtggtcgat ttcccggccc cgttcggccc gagaagcgca taaaaatcac ccgcttcgac
     1081 ctgcaaatct attccacgaa gcgcctgaac gccgcctgga tagttttttt taagctgttg
     1141 aagttccagt gcaatggtca taaattttta cttaccttac gttcttacac tttatctgtg
     1201 gtttaaatcg tcccggagtt gccctatatt agccaaacgt aattatttgg ttacaggtcg
     1261 ttaacctcca tgaaagacat agatacactc atcagcaaca atgcactatg gtcaaaaatg
     1321 ctggtggaag aggatcccgg gttttttgag aaactggcac aagcgcaaaa accgcgcttt
     1381 ctatggattg gatgttccga cagtcgcgtt cctgcagaac gtttaaccgg tcttgagccg
     1441 ggcgaactct ttgttcaccg taatgttgct aacctagtca ttcacaccga cctgaactgc
     1501 ctttccgtgg ttcagtatgc agtggatgta ctcgaagttg aacacattat tatctgtggc
     1561 cactacggtt gcggcggcgt acaagccgcc gttgaaaacc cggaactggg gcttatcaac
     1621 aactggctgc tgcatatccg cgatatctgg ttcaaacata gctcattgct cggcgaaatg
     1681 ccgcaagagc gccgtctgga taccttgtgt gaactgaacg tcatggaaca ggtgtataac
     1741 ctgggccact ccaccattat gcaatcagcg tggaaacgcg ggcagaaagt taccattcac
     1801 ggctgggcct acggcattca cgacggcttg ctgcgtgatc tggatgttac cgccaccaac
     1861 cgcgaaaccc ttgagcaacg ttaccgtcac gggatttcca acctcaagct gaaacacgcc
     1921 aaccacaaat aaaaatgcca tgccggatgc aacacatccg gcaacttcac acttactcgt
     1981 ccagcagaat cactttgcca atatacggca gatgacggta acgctgtgcg taatcaatgc
     2041 cgtaacccac cacaaactca tccgggatcg agaaaccgat aaattcaacc gggacgttca
     2101 cttcacgacg ggacggttta tccagcagcg tacaaatcgc caacgacttc ggttcgcgca
     2161 ggcttaagat ctcacgcact ttcgacagtg tattccccga gtcgatgata tcttcaacaa
     2221 tcagcacgtc cttgccacgg atatcttcat ccagatcttt gaggattttc acatcacggg
     2281 tggtggacat gccgctaccg tagctggagg cggtcataaa gtcgacttca tgagatacct
     2341 gaacttcacg gcacaggtcc gccataaaca taaatgagcc acgcagcaga cccaccagca
     2401 ccatatcgct gccgctgtct ttgtaacgct cagtaatctg acgacccagt tcggcgatac
     2461 gcgctttaat ctccgcttcg gggatcatta cttctacagt atgtttcata tctctaacca
     2521 tatgatttaa aaacaaatca cttcgcacgg gcaggttttg gcaccgcaac aaagcgcaag
     2581 acgcgcagta taccagtaaa actattcatt cgcggaatct gttaataaag ctcgaattgt
     2641 gatgacggtc acacatgtta aaacctataa ttaattgcta ctaaaatatt aatgaattga
     2701 aatggtgtct ctttatggca attaacaata cagcctcgcg acgattactc gtcacgctaa
     2761 cagccctttt tgcagcgctt tgcgggctgt atctactcat tggcggaggc tggctggtcg
     2821 cgattggcgg ctcctggtac taccctatcg ctggccttgt gatgctcggc gtcgcctgga
     2881 tgctgtggcg cagtaaacgc gccgcgcttt ggctatacgc agccctgctg ctcggcacca
     2941 tgatttgggg cgtctgggaa gttggtttcg acttctgggc gctgactccg cgcagcgata
     3001 ttctggtctt cttcggcatc tggctgatcc tgccgtttgt ctggcgtcgc ctggtcattc
     3061 ctgccagcgg cgcagttgcc gcactggtgg tcgcactgct gattagcggt ggtatcctga
     3121 cctgggccgg atttaacgat ccgcaggaga tcaacggcac cttaagcgcc gatgccacac
     3181 ctgccgaagc tatctccccc gttgccgatc aggactggcc tgcgtatggc cgcaatcagg
     3241 aaggtcaacg cttttcacca ctgaagcaaa ttcacgccga taacgtccac aagctgaaag
     3301 aagcctgggt gttccgtact ggcgatgtga agcagccgaa cgatccgggt gaaatcacca
     3361 atgaagtgac gccgattaaa gtgggcgata ccctttacct gtgtaccgct caccagcgcc
     3421 tgtttgcgct tgatgccgcc agcggcaaag agaaatggca ttacgatcct gagctgaaaa
     3481 ccaacgagtc tttccagcac gtaacctgcc gtggcgtctc ttatcatgaa gccaaagcag
     3541 aaacggcttc gccggaagtg atggcggatt gcccgcgtcg tatcattctt ccagtgaatg
     3601 atggtcgcct gattgcgatt aacgctgaaa acggcaaact gtgcgaaacc ttcgccaata
     3661 aaggcgtgct caatctgcaa agcaatatgc cagacaccaa accgggtctg tatgagccga
     3721 cttctccgcc aattatcacc gataaaacca tcgtgatggc cggttcagtc accgataact
     3781 tctcaacccg cgaaacgtct ggcgtgatcc gtggttttga tgtcaacacc ggggagctgc
     3841 tgtgggcttt tgatccgggc gcgaaagatc cgaacgcaat cccgtcagac gaacacacct
     3901 ttacctttaa ctcaccaaac tcgtgggcac cagcggccta tgacgcgaag ctggatctgg
     3961 tctatctgcc gatgggcgtg accacgccgg atatctgggg cggtaaccgc acaccggaac
     4021 aggaacgtta tgccagctcg attctggcgc tgaatgccac taccgggaaa ctggcgtgga
     4081 gctaccagac cgttcaccac gacctgtggg acatggatct tccggcacag ccgacgctgg
     4141 cggacatcac cgttaatggt cagaaagtgc cgattattta cgctccggcg aaaaccggta
     4201 acatttttgt gctcgatcgt cgtaatggcg aactggtggt tccggccccg gaaaaaccgg
     4261 ttccccaagg tgctgccaaa ggcgattacg ttacccctac tcaaccattc tcggaactga
     4321 gcttccgtcc gacgaaagat ttgagcggtg cggatatgtg gggagccacc atgtttgacc
     4381 aactggtgtg ccgcgtgatg ttccaccaga tgcgctatga aggcattttc acgccgccat
     4441 ctgaacaggg tacgctggtc ttcccgggta acctggggat gttcgaatgg ggcgggattt
     4501 ccgttgatcc aaatcgtgaa gtggcaattg ccaacccaat ggcactgccg tttgtttcga
     4561 aactgctccc gcgtggtcct ggcaacccga tggagcagcc gaaagatgcc aaaggcacgg
     4621 gtacggaatc cggcattcag ccacagtacg gtgtaccgta tggtgtcacg ctcaacccgt
     4681 tcctctcacc atttggtctg ccatgtaaac agccagcatg gggttatatc tcggcgctgg
     4741 atctgaaaac taatgaagtg gtgtggaaga aacgtattgg tacgccgcag gacagtatgc
     4801 cgttcccgat gccggttccg gtgccgttca atatgggtat gccgatgctg ggcgggccaa
     4861 tctccacggc gggtaacgtg ctgtttatcg ccgctacggc agataactac ctgcgcgctt
     4921 acaacatgag caacggtgaa aaactgtggc agggtcgttt accggcgggt ggtcaggcaa
     4981 caccgatgac ctatgaagtg aatggcaagc agtacgtggt gatctccgca ggcggtcacg
     5041 gttcatttgg tacgaagatg ggcgactata ttgtggctta tgccctgccg gatgatgtga
     5101 aataaccata atgcccggag agatccgggc atatttccga atacggtctt tttataccgt
     5161 aaaccctaac atcatccccg tatcttcatg ctccagcaga tggcagtgcg ccatataagc
     5221 atgttctttc ggtgcgttgt gattaaactt caccagcact tcactgacat tgccttctac
     5281 cttaacggta tctttccagc ccgcgcgatg agccgctggc cgtttgccat tttctgacaa
     5341 gatacggaac tgcgtgccgt gaatatggaa cggatgcagc atcatgtcgc ccacgccaga
     5401 gataacccaa cgttcgtact gccctttcgc cgccgcaaac atcggcttat tcatatcaaa
     5461 cgcctgaccg ttgattttat tggcatggtg gaaatcgaac ttcccgccgt ggttcatatg
     5521 attcatattg ccgtgcccca tatggcccat catctggctg tgatccattc cggccatcgc
     5581 ctgatcgcca tatttctcca ttagcatctg catccccatc atatcgagca tcgggtccat
     5641 agaaagttgc agcttgcgta ccgtcagccc ttccagcgaa ggtaacgcag gcaggctact
     5701 taatgtgtca ggcaaagcac cggaggcact aatagcaatc ggctgaatcc gcattaccgg
     5761 atgcggcttg tcaaacggtg caatcgccat ccccatctgg ctgaccggca gcgtcaccag
     5821 gtcaaagggt ttgttgtcgt taacctccac cagcacttca aaacgctcgc ccatcagcac
     5881 cggcagctcg ctcaccttca ccggttcagg tagcagacca ccgtcgctgg caatcacata
     5941 cagcgggcga ttgtcgctgg tggcgaaatt gagcgagcgg gcattacagc cattgagcaa
     6001 acgcaggcgc agccaaccac gcggggcagc gtgttgtggg tagattgcac cgttggtcag
     6061 cagcgtatcg ccaaaccagc ccacggcggc ggtcatcaca tccagttgat aatcaatctg
     6121 cccgtcggcg ctaaatttct tatcctgaac gatcaccgga acatcatcga taccccactg
     6181 ttttggcagc attaatttca ggatctcgtc atcttcaatc accaccagac cagccagccc
     6241 catcgccacc tgtcgcccgg tcttgccatg ttgatgtgga tggaaccagc aggtagcggc
     6301 aggttgatca acgttcaacg tcaccgagcg cttgccacct ggcggaataa ttccctgtgg
     6361 gccgccgtcg acgtcacccg gtacttccag cccgtgccag tgcaacgtcg tctcttccgc
     6421 cagttggttg tagatatcaa ccgttaccgc tttgccgcgc tgtaatttca ccgccggccc
     6481 cagcagattg ccgttatagc cccaggtagt tgcagttttc tcgccaaagg tggactgacc
     6541 tgcgccaata gttaactgaa tgcgattacg ggcatcggtc gtgagcaaat cagggatcgg
     6601 taacgttggg cgttccgccg caaagactgc acggctccac agtggcaagg ctgaagccac
     6661 acccagtgcg acggaatatt tcaagaaatc acgacgttgc atagttattt ccttattctt
     6721 aagcaggcga aacaaaatca aacgttgagc atagtccttc cccttacggg aaggtcaagc
     6781 cgcagacaca atttaatcga caataatggt cgtcgcctcg ctcgcagtgt gctaacgttt
     6841 atcttcttta agcccctggt agaagcaatg aagacgtttt tcagaacagt gttattcggc
     6901 agcctgatcg ccgtttgcgc aaacagttac gcgctcagcg agtctgaagc cgaagatatg
     6961 gccgatttaa cggcagtttt tgtctttctg aagaacgatt gtggttacca gaacttatct
     7021 aacgggcaaa ttcgtcgcgc actggtcttt ttcgcccagc aaaaccagtg ggatctcagt
     7081 aattacgaca ccttcgacat gaaagctctc ggtgaagaca gctaccgcga tctcagcggc
     7141 attggcattc ccgtcgctaa aaaatgcaaa gccctggccc gcgattcctt aagcctgctt
     7201 gcctacgtca aataatccct gatacctttt tgtagaaata ttgaccgtgc atccgcggtc
     7261 aatgttagct attatgttgc gccctttttt tacgggtgtt aacaaaggag gtatcaaccc
     7321 atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc agtttgggca gtactttgcg
     7381 gtagataacg ttctgtatca tgaaaagacc gatcaccagg atctgatcat ttttgagaac
     7441 gctgcatttg gtcgcgtaat ggcgctggat ggcgtagtac aaaccaccga gcgcgacgag
     7501 tttatctatc atgagatgat gacccatgtt ccgctactgg cccacggtca cgcgaaacat
     7561 gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg aagtaacccg acataaaaac
     7621 gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg tgtcgttctg ccgtcagtat
     7681 ctgcccaacc ataacgccgg tagctacgac gatccgcgct ttcaactagt gatcgacgat
     7741 ggcgtcaatt tcgttaatca aaccagccag acctttgatg tcattatctc cgactgcacc
     7801 gatcctatcg gtcccggcga aagcctgttc acctcagcat tttatgaagg ctgcaaacgt
     7861 tgcctgaatc ctggcggtat cttcgtcgca caaaacggcg tctgcttttt acagcaggaa
     7921 gaagccatcg acagccatcg caaactcagc cattacttca gcgacgttgg cttttatcag
     7981 gcggcgatcc cgacctatta cggcggtatc atgacttttg catgggcgac agataacgac
     8041 gccttacgcc atctctcaac cgaaattatt caggcgcgtt ttctcgcttc tggcctgaaa
     8101 tgccgttatt acaatccggc aatccatacg gcagcttttg ccttacctca gtatctgcaa
     8161 gacgcactgg cttcacagcc gtcctaagga gaagataaga aattgaaaaa actgaaactg
     8221 catggcttta ataatctgac caaaagtctg agtttttgta tttacgatat ctgctacgcc
     8281 aaaactgccg aagagcgcga cggttatatt gcttatatcg atgaactcta taatgccaac
     8341 cgtctgaccg aaatcctgtc agaaacctgt tccattatcg gggccaatat tcttaacatc
     8401 gcccgccagg attacgaacc acagggtgcc agcgtcacta ttctggtgag tgaagaaccg
     8461 gttgacccga aactcatcga caaaacagaa caccccggcc cactgccaga aacggtggtt
     8521 gctcatctcg ataaaagtca tatttgcgta catacctacc cggaaagtca tcctgaaggc
     8581 ggtttatgta ccttccgcgc cgatattgaa gtctctacct gcggcgtgat ttctccgctg
     8641 aaggcgctga attacctgat ccaccagctt gagtccgata tcgtaaccat tgattatcgc
     8701 gtgcgcggtt ttactcgcga cattaacggt atgaagcact ttatcgacca tgagattaat
     8761 tcgattcaga actttatgtc tgacgatatg aaggcgctgt atgacatggt ggatgtaaac
     8821 gtctatcagg aaaatatctt ccataccaag atgttgctta aagagttcga ccttaagcac
     8881 tacatgttcc acaccaaacc ggaagattta accgacagcg agcgccagga aattaccgct
     8941 gcgctgtgga aagaaatgcg cgagatttat tacgggcgca atatgccagc tgtttaacgg
     9001 ctctggcgga gctcccaggc tccgccagat ttatttactt ctgctgcacg aaattgcggt
     9061 aagccgccac gacctgcaga aaatcctcaa cgccgcatag cgacaggctt tcttcgtcgt
     9121 agtagttcat cccctcttcc atttcatcgc cagcgaattc cagttgattg gcgcgaacca
     9181 tcacctcttc agcgtccatc cacagggtgt attcatgccc tgcccgttgc caggaccgtt
     9241 cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc aagcaaggcc aggttttctt
     9301 tcacctcttc attaaaccag tgcccgacca cttcatgccc catggacata cgcaccttta
     9361 ccactccggt aatatcgcgc agaaattcgt aatccatagt gtgttcctct gctcccggca
     9421 atgcgccgaa atcgcatcgc gccattgatg taattatcgc agccttaccg gagaaaaaca
     9481 gcggaacggc gatagtttag aaataaaaaa tgcccgggaa tctcattcac cgggcattgt
     9541 gtcgtttatg cgcagcgcgt gcgcttactt attacaccgc agtctggaaa atcaccccat
     9601 cggctttttc ggtgtactga gaaagctggt tgaagttcag ataacgataa gtatcaacgg
     9661 cagttttatc tacttgcgca acgtaggtct ggtactcttc cggcgtcggc agtttgccaa
     9721 tcagcgccgc aacagccgcc agttctgcag aagccaggaa gacattcgcg ccagtaccca
     9781 aacggttcgg gaagttacgg gtagaggtgg aaaccaccgt cgcaccgtct gctacacgcg
     9841 cctggttacc catacacagg gaacagcccg ggatctcgat acgagccccg ctcttaccga
     9901 agacgctgta gtagccttct tccgtcagtt gcgcggcgtc catacgggtt ggcggtgcca
     9961 cccacaggcg ggtcggcaac tgacctttgt gcgcatccag cagtttacca gcggcacgga
    10021 agtgaccgat gttagtcatg caagaaccga taaatacttc gtcgatcttc tcgccctgta
    10081 ccgcagacag cggacgtgcg tcatccggat cgttcggagc acacaggatt ggctctttaa
    10141 tctccgccag atcgatgtcg atcactgccg cgtattccgc atctgcatcg gcttccagca
    10201 gctcaggatt cgccagccat ttttccatgc cctgaatacg gcgttccagg gtacgacgat
    10261 cgccgtaacc ttccgcgatc atccacttca gcaggacgat gttggagctc aggtattcga
    10321 tgatcggttc tttgttcagc ttgatagtac aaccagcggc agaacgctcg gcggacgcat
    10381 cggttagctc aaaggcctgc tcaactttca gatccggcag accttcaatt tccagaatgc
    10441 gaccagagaa gatgtttttc ttgcctttct tctcaacggt cagcagacct tgtttgatcg
    10501 catacagcgg aatggcgtgt accagatcgc gcagggtgat gcccggctgc attttgcctt
    10561 tgaagcgcac cagaacggat tccggcatat ccagcggcat tacgccagtt gcggcagcaa
    10621 acgccaccag accagaaccc gccgggaaag agataccgat cgggaaacgg gtatgggagt
    10681 caccaccggt accgacggta tccggcagca gcatacggtt cagccaggag tgaatgacgc
    10741 cgtcacccgg acgcagcgac acaccgccac ggttcataat gaagtccggc agcgtgtggt
    10801 gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac tgcatcacca
    10861 ggtcagccga gaagcccagg cacgccaggt ctttcagttc atcacgggtc atcgggccgg
    10921 tggtgtcctg agagcctaca gaagtcattt tcggttcgca gtacgcgccc ggacgaatgc
    10981 ctttcacgcc acaggcacgg cctaccattt tctgcgccag cgagaagccg cgatcgctct
    11041 cagcgacatc tttcgcctga cggaacacat cactgtgcgg cagaccaagt gcttcacgcg
    11101 ctttggtggt caggccacgc ccgataatca gcggaatacg gccgccagca cgcacttcat
    11161 caatcagcac gtcggttttc agttcgaagg tcgccagcag ttcgcctgtt tcgtggttac
    11221 gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc catgttcagg ttagagacgt
    11281 tgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt aaagaagatg ggtgcaattt
    11341 taccgccgag gcacaaacca ccgccgcgtt tgttcggcac atgtggaata tcatcgccca
    11401 taaaccacag aacggagttc gtggcggatt tacgcgatga acccgtaccc acaacgtcac
    11461 cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc aatctgtttg atcgggccaa
    11521 caacacctgg ctggtctggc tcaatacctt cacgggcgtt tttcagcatc gccagcgcgt
    11581 gcagtgggat atccgggcgt gaccacgcat ccggtgccgg agaaaggtca tcggtgttgg
    11641 tttcgccagt gactttaaag acggtaacgg tcagtttttc agccagcgcc gggcgattca
    11701 ggaaccattc ggcatccgcc caggactgca ttacctgctt cgcatattcg ttgcctgctt
    11761 tcgctttctc ttctacgtca tagaagttat cgaacatcag cagcgtgtga gaaagtgctt
    11821 tggcagcaat aggtgccagt ttggcatcat ccagcgcgtc gatcagcgga tgaatgttgt
    11881 aaccaccctg catggtgccc agcagttcga tagctttttc cggagtcagc agaggggatt
    11941 tagcttcgcc tttagcgata gcagccagga agcctgcttt tacataggcg gcttcatcga
    12001 cgcctggggg aacacggttg gttaacagat ctaacaggaa ttcttcttcg cccgcgggcg
    12061 ggtttttcag cagctctaca agcgcggcca tttggtttgc atccaggggt ttgggcgcaa
    12121 tcccctcagc ggcacgctca gctacgtgct tacggtattc ttctagcacg acggttctcc
    12181 tcgctctcat tgtcatagtg cggcaggcga ttctcttcac gctcctgtga gacagcagtt
    12241 tgtagggtaa atgcccagta ccgcaacggg cagaatagca ggattttgac gacgtgttaa
    12301 tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg tctagagatt
    12361 gtttccctcc gtaaaaactt cgttaattcg catggtgata atcacctttc tcaacgaatc
    12421 ccaagattag tcaaaattta aactaccgcc tctttatact cggattcaca gcacctgcgg
    12481 gtggcagttc gccgaccatt gcgatttcct tgagatccga attatgaaaa tgactttgcc
    12541 gtttaaaccc catgtgctgg cgctaatttg cagtgccggg ctttgtgctg cctctgccgg
    12601 gctatatata aaaagccgca cagtggaagc gcctgtagaa ccgcaatcga cacaactggc
    12661 tgtgtctgac gctgccgctg ttacgcttcc tgcaacggtt tccgcacctc ccgtaatacc
    12721 cgcagtcgtc aaatccgcat tcagcactgc acaaatagat caatgggtcg cacccgtcgc
    12781 gctgtatccc gacgccctac tttcgcaggt gctgatggca tcaacctatc cgacaaacgt
    12841 tgctcaagca gtgcaatggt cgcacgataa tccacttaaa caaggcgatg ctgctattca
    12901 ggcggtatct gaccagccgt gggacgccag cgttaaatca ctggtggcct ttccacaatt
    12961 gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg cttttctggc
    13021 gcagccgcag gacgtgatgg actcggtaca gcgattgcgg caactggcgc aacaaaccgg
    13081 ctcgctgaag tcatcaaccg aacagaaagt tattaccaca acgaagaaag ctgtaccggt
    13141 aaaacagaca gtcacggcac ccgtcatacc atccaatacc gttttaactg ccaaccccgt
    13201 cattacagag ccggcaacaa ccgtcatttc cattgagccc gccaatcctg atgtggtcta
    13261 tattcccaac tacaacccaa ccgtggttta cgggaactgg gccaatactg cgtatccgcc
    13321 ggtttatctg ccaccaccag ccggagaacc gtttgttgac agctttgtgc gcggattcgg
    13381 ctatagcatg ggcgttgcta ccacgtacgc actattcagc agcatcgact gagatgacga
    13441 cgatcatgac catcatcatc atgacgatga tgattatcat caccacgatg gcggtcatcg
    13501 tgacagtaat ggctggcaac acaacggcga caacatcaat atcgacgtca acaatttcaa
    13561 ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca atccaaacta
    13621 ccgtaatggt gtgccctatc atgatcagga tatggcaaag cggtttcatc aaaccgatgt
    13681 caacggcgga atgagtgcca cgcagctacc tgctccaaca cgcgacagcc agtgtcaggc
    13741 ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacac gagataccca
    13801 acgtcaggca gcggcacagc ggtttaatga agctgaacac tatgggagct atgacgactt
    13861 ccgcgacttc agccgtcgcc aaccactgac ccagcagcaa aaggacgccg ctcgtcagcg
    13921 ttatcagtcg gcctctcctg agcagcgcca ggcagtccgc gagaaaatgc agactaaccc
    13981 gcagatccag cagcgaaggg aggcggcgcg ccagcgtatt cagtccgcct cgcctgagca
    14041 gcgccaggcg gttcgcgaga aaatgcagac taacccgcag aaccagcagc gaagagaggc
    14101 agcgcgtgag cgtattcagt ctgcaactcc cgaacagcgc caggtgttta aggaaaaagt
    14161 acagcagcgc ccactgaacc aacagcaacg tgataacgcc cgccagcgta tacaatcggc
    14221 atcacctgaa caacgtcagg tttttcggga gaaagttcag gagagccgcc cacaacgtct
    14281 aaacgacagt aaccatactg tcaggctgaa taacgagcaa cggtcagcag tacgcgaacg
    14341 tctctctgaa cgtggagcaa gaagactgga aaggtaaatt gcagacgtaa aaaaagcggc
    14401 gtggttagcc gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta cttcttcttc
    14461 gctttcgggt tcggcaggtc ggtaatgcta ccttcgaaca cttctgccgc caggcccaca
    14521 gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc
    14581 atttcgattg ccaggccgat ttcacccagc agctcgccgc cgttggtacc aacaatcgca
    14641 ccaccgatca cacggtgaga ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag
    14701 tcagaagcga tagcacgacc agaagcagcc cacgggaagg tggcggtttc atagctgatg
    14761 cctttctctt tcgcttcttt ctcagtcaga cctacccatg caacttctgg ttcggtatag
    14821 gcgatggacg ggataacttt cggatcgaag tagtgtttct taccggcgat aacttcagcg
    14881 gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg gctgaccgac gatatcgccg
    14941 atagcaaaga tgtgcggtac gttggtacgc agctgtttgt caacgcggat gaaaccacgg
    15001 tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg cacacgacca
    15061 atcgctacca gcacggcgtc gtaacgctgc ggttcagctg gtgctttttt gccttccatc
    15121 gtcacataaa taccgtcttc tttcgcttca acggcggtaa ctttggtttc cagcatcagg
    15181 ttgaatttct tgctgatacg cttggtgaag actttaacga tgtctttgtc agctgccggg
    15241 ataacctggt cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg gtaaacggtg
    15301 cccatttcca gaccgatgat accgccaccc attaccagca ggcgttctgg tacttctttc
    15361 agttccagcg cgtcagtgga gtcccagata cgcggatctt catgcggaat aaacggcagt
    15421 tggatcgggc gagaacccgc tgcaatgatc gcattgtcga agttgatcac ggttttgccg
    15481 ttctcacctt caacttccag ggtgttagcc ccggtgaatt tacccagacc gttgaccact
    15541 ttgactttgc ggcctttcgc cataccagcc agaccaccgg tcagctgatt gatcactttc
    15601 tctttccagg tacgaatctt gtcaatatcg gttttcggtt cgccgaagac gataccgtgt
    15661 tcagccagcg ctttggcttc ttcgataact tttgctacgt gcagcagtgc tttagaaggg
    15721 atacagccga cgttcaggca aacaccgcca agggtgttgt aacgttctac gattacggtt
    15781 tccagaccta aatcagcgca acggaaggca gcggagtaac ctgcggggcc tgccccaagt
    15841 accacgacct gagttttgat ttcagtactc atcatgacct ctatatattt atctccggcg
    15901 gtcatacccg tcgtctttca ggccgcagcg gcgttagctt cacgccctca ccccagtcac
    15961 ttacttatgt aagctcctgg ggattctggc gcttgctacc ttgctgcacc cagaaatcca
    16021 tagggtatgt gaagtaatac cctaaccacc accgggtcgt tctatccgtc ggtattttac
    16081 aaaattgtta acaattttta aacaacaaac ggcaaccgat ttgtctattc gctaataacc
    16141 tcaatacatt catgagatta ccagaaaaaa gccggccgtt gggccggctc ttttacttac
    16201 atcaccagac ggcgaatgtc agacagcgtg ttgttaatga tggtaatgaa acgggcacca
    16261 tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa tcggcagcat cagacgcggc
    16321 acgaactctt taccattcca caccggctcc atcgcggact tggaaacgcc gaggatagcc
    16381 acttccggcg cgttcacaat cggcgcgaag tgggtagtac ccaggccgcc gatgctggag
    16441 atggtgaagc aaccgccctg catttcgccc gcagtcagct taccgtcacg cgctttctta
    16501 gaaatagtca tcagctcgcg agacagctcg atgatgcctt tcttgttgac gtctttgaat
    16561 accggaacaa ccagaccgtt cggggtatcc accgccacac cgatgttgat gtatttcttc
    16621 agggtcagac gctgaccgtc ttccgacagc gaactattga agcgaggcat ctgctcaaga
    16681 gctgcagcaa cggctttcat gatgaagaca accggggtga tcttcacatc cagcttacgt
    16741 ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca gctcggtgat atcggttttg
    16801 tcgaagtggg taacatgcgg gatcatcacc cagttacggc tcaggttagc accagaaatt
    16861 ttctggatac ggcccagttc cacttcttcg atttcgccaa acttgctgaa gtccaccttc
    16921 ggccacggca gcatgccagg gataccaccg ccagtcgccg ccggagctgc ttctgcacgt
    16981 ttgatagctt ctttcacgta agcctgaacg tcttcgcgca ggatacgacc tttacggcca
    17041 gtgcccttca ctttcgccag gttaacgcca aactcgcgtg ccagacggcg gatcagcggg
    17101 gtcgcgtgaa cgtaagcgtc gttttcagca aattcagatt tgccttccgc tttcgcagcc
    17161 ggagcttcag cttttgctgc cggagccggc gctgccgctt cctgtttcgc aggagctgcc
    17221 gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg aaccagtttt cactttatcg
    17281 ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccggaac ttccatagaa
    17341 gctttatcgc cttctacggt aatcagtgac tgttcagcgg caactttgtc gcccactttc
    17401 accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt aacttctttc
    17461 acgccagctg ctggtgcagg ggtcgctgcc ggagtcgctt cctgtttagc cgccggagct
    17521 gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt agacactttg
    17581 tcacccacgt tcactttgat ctctttcacg gtacctgcaa acggagccgg aacttccata
    17641 gaagccttgt cgccttctac ggtgatcagc gactgttcag cttcaacttt atcgccaact
    17701 ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac gttaacgtct
    17761 tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc ttctgcctga
    17821 gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag tgcgccggtc
    17881 tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg cggagatgga
    17941 acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc ttcaactttg
    18001 tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat gtccggtact
    18061 ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta actttatctg
    18121 catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt tcgccacgtt
    18181 tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg aagtggtgac
    18241 gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg cgatagtcgt
    18301 cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg gtagatgcca
    18361 ctgccggcgc gtcgttcatc acctgagcga tatacggaac gcgcggagtt tccagcggat
    18421 gcagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg aaggaggtca
    18481 cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc gcttcacgga
    18541 cgtgacgcag gatggaaccg gagcccagca gctgaacttt acctttgcta ccttcaatgg
    18601 tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc attgccggca
    18661 tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt ttttcaccgt
    18721 acatacgctc cagaccgtca tgcataatga cagcaacttc gtaagcgtaa gccgggtcgt
    18781 aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct tcgtgctgca
    18841 gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag ccacgcgctt
    18901 gctggtctcc agccgcccag cacagatcgc cgatacgctg gaagccgaac atcgagtaat
    18961 agatgtagaa tgggatcatc ggcagattgt tcgtgctgta agaggtcgcc gctgccagcc
    19021 aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct ttctcgtctt
    19081 ctttatagta agcaacctgc tcgcggtcct gcggggtgta ctgctgaccg ttcgggctgt
    19141 aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg tcggcgatga
    19201 tcggtaccag acgatctttg atcgacttgt tcttcagcat cacgttcaga gcacgaacga
    19261 aagcgatagt ggtagagagc tctttgctct gctcttccag cagcgcgccg aagtcttgca
    19321 ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga taaccgtgca
    19381 gtttctgacg ctgagcgtgc agataggtat gctcttcaga accttccggg aaggtgatgt
    19441 acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg cggatgtgac
    19501 gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct tcagccgcgt
    19561 cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg cctttggttt
    19621 cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca cggttcagtg
    19681 cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga tatttaccga
    19741 agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag tcgccgtcaa
    19801 cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc agttcatccc
    19861 aacggctacc ccacatcact ttgatcacgt tccagccagc accttcgaag atgccttcca
    19921 gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc aggttacagt
    19981 tgataacgaa aaccaggtta tccagttttt cacgggtagc gatggtgatc gcacctttgg
    20041 attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt ttagaggtat
    20101 ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata gcaccaatcg
    20161 gacccagacc catagatacg gtcgggaact gccagaattc cggcatcagt ttcgggtgcg
    20221 gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc tgctcctgag
    20281 tcagacgacc ttccaggaaa gcacgggcgt aaacgcccgg ggagatgtgg ccctggaagt
    20341 aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta aagcacacat
    20401 cataaatggt tgcggaagac tggaaggacg ccatgtggcc gcccagttcg aggtcttttt
    20461 tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga atacggcgtt
    20521 ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg ttgatgtagt
    20581 tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca gcaagcagtt
    20641 ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat tcgatcgcct
    20701 ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct gacatgggtt
    20761 attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga actctcgcca
    20821 gaaagctcaa taagacaggt tctacgttta gttaccgcgc ttttatatgc gcttgattta
    20881 caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg ctccagacga
    20941 cgcagagaac gctcacggcg gctctcttca cggcttctgt cgagcaaaat ttcttcgata
    21001 aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc cataatcgct
    21061 tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg cgaatagagc
    21121 aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct tagcagatga
    21181 agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag tacggcgttt
    21241 gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg gagttcacgg
    21301 atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc gataccttcc
    21361 agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga gagcagctcc
    21421 accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc gccaccctgg
    21481 cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa ggagggacgg
    21541 gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc cgggcggaga
    21601 gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca catcggagag ttttggttgg
    21661 cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt caattggtct
    21721 taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt ccatacaggt
    21781 tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata acgtttcaaa
    21841 gttgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa cgattattaa
    21901 actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt aagtcaataa
    21961 aaacgaattt aaattcactc tgcatattga gaggggttga ggctgagctt tacaaacggt
    22021 ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc tgccacatac
    22081 cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcctt gatgtaaata
    22141 aatcaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag atcaaaaaaa
    22201 caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag ctaaagcgcg
    22261 gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg acagggctat
    22321 tcctaggtag cgcctccgta atacagtccg cagggccagg gattatcctg ggttacgcca
    22381 ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg gtcgaagaac
    22441 ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt gccggtttcg
    22501 cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag ctgactgccg
    22561 tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct gccgccgtat
    22621 tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc gagatggagt
    22681 tctggtttgc cattatcaaa gttattgcgg tagtagcgat gatcatcttc ggcggctggc
    22741 tgctgttcag tggcaacggt ggcccgcagg cgagcgttag caacctgtgg gatcaaggtg
    22801 gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc atgttctcgt
    22861 tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg gagcaaagta
    22921 tcccgaaagc aactaaccag gttatctacc gcatcctgat tttctatatt ggttcgttag
    22981 ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt ccgtttgtgc
    23041 tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc gtggtactga
    23101 ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg ctgtttggtc
    23161 tggcacaaca ggggaacgcg ccaaaagcgc tggcgtctgt cgataaacgc ggcgtaccgg
    23221 ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt aactatcttg
    23281 ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg gtgatcaact
    23341 gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa caaggcgtgg
    23401 taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg ctgtttatgg
    23461 cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta tacctgatcc
    23521 cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc gccaaagccg
    23581 taaaagcgca ttaatctctc tacgccctca cccgtacagg gtgagggcaa taatctctac
    23641 accaacgccc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa cgaggttttc
    23701 ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg ttcacgcgcc
    23761 agagagaact gcgccagacg cgttaatacc tgatattgcg aagtatggaa atcacccagc
    23821 gaagcaaacc aggccggtaa cgctttctca ccatgaccga tcaaggcata taccacaccc
    23881 gcaagacgaa ccggcaccca atccagtaca tgaagcactg catcaatgcc ggactgtaaa
    23941 cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa gaacgcatac
    24001 cccatcagcg taacgggtcc ccaggttccc cccacaatca gccagaacag cggtgcaaga
    24061 taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata ctcacgttcg
    24121 tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt ggcacgggca
    24181 tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg aactttacct
    24241 gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac gttgaacaat
    24301 actccctgca atgcgcgtaa cagtaaaaaa gtcacaccca tcgcaataat ggtcatgcct
    24361 aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag acgatgatca
    24421 agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag taaggttgta
    24481 aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa tcaaatgcag
    24541 gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg ttattagcga
    24601 tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga tactgcgcat
    24661 cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa tcattgcagc
    24721 gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg aaaggaacat
    24781 actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc aaatgggcga
    24841 tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat gcgtcgatcc
    24901 acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc agggtgggtg
    24961 tttcgtcatc caggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg ccaaccagcc
    25021 acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc cggttcagag
    25081 tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc cgcctcgccg
    25141 ctacaaccct gacacccgac gtgacgagct gctggaacgc attaatctcg atatccccgg
    25201 cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca acaatgacat
    25261 tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc acggtgatca cccgcgagaa
    25321 tggcgtattt tgtggcaaac gctgggttga agaggtgttt attcaactgg caggcgacga
    25381 tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc aacccttgtt
    25441 cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc ttaattttgt
    25501 gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac tggaaggcac
    25561 caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag ctctgaaata
    25621 cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg ccttcctgat
    25681 caaagaaaac catattattg cctccggctc agtgcgccag gcggtcgaaa aagcgtcctg
    25741 gctgcatccg gatgcgccag tagaagtcga agtagagaat ctggaagaac ttgatgaggc
    25801 cctgaaagcc ggagccgata tcatcatgct ggataacttc gaaacagaac agatgcgcga
    25861 agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg tcactgacaa
    25921 aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg cgctaactaa
    25981 acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag atttatccgc
    26041 tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct cgcaaatttc
    26101 ttgtctttcg ctgcaacgaa ataaaaaacc tcggaatctg ctttcccgtt tcgttttttg
    26161 cccctcgcct gtgctgatgg cgtttgccaa agtagcacca accaaatcaa ggagcgaaac
    26221 agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt ggcatcattg
    26281 ccattttaag ctccattggt attcccgctt atcaaaacta cctgcgcaaa gccgcactca
    26341 ccgacatgct acaaaccttt gtgccttacc gtaccgccgt agagttgtgc gcgctggaac
    26401 atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct accaccaccc
    26461 gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc gctgaccggg caagaaagtc
    26521 tcaatgggct aagcgtcgtc atgacgccag gttgggataa cgcaaacggc gtcaccggct
    26581 gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc gaagatgtct
    26641 tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc acggccctgt
    26701 gcctgcgtta tcagggagtc ttgctggatg ccagcgaaga agtggttcat gttgcggtgg
    26761 tcgatgcccc ctcacatgag ttgctggacg cattgcattt cgctaccacc aaacgtattg
    26821 agatcacctg ctggacgcgc caacaaatgg aaggtcacgc cagtcgcaca caacagacat
    26881 tacccgtagc tgttcaggag aagcatcagc ccaaagcaga attgctgact cgaacgttac
    26941 aatctgcgct ggaacaacgc gcgtctgata ttcatatcga accagcggac aatgcctacc
    27001 gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca ccggatgccg
    27061 gagtcgcatt aacagccaga ttaaaagtgc tgggaaacct ggatattgcg gaacatcgcc
    27121 tgccgcagga cgggcaattc actgtcgaac tggcaggaaa caccgtctca tttcgtattg
    27181 cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag caggtgagcc
    27241 aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac tttgctcatg
    27301 ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagt ggcaaaacgg
    27361 tcacctggtg ttgtccacgc tacacaccaa ttccaccact gaaacgctgg tacgtttaca
    27421 gcaaatgggg gtcgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg
    27481 tctggtacgt aaactttgcc cacattgtcg ccgacagcaa ggggagccca tccatattcc
    27541 agacactgta tggccgtcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta
    27601 ccacggtttt tatggtcgta ccgccttgtt tgaagtcctg cccattacac cagccatacg
    27661 ccagcttatt tccgctaata ctgaagttga atcgctggaa acgcacgccc gacaggcggg
    27721 tatgcgtacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga
    27781 agagttaatc cgcgtactgg ggatgccgca tggcaagtaa gcaactctgg cgctggcatg
    27841 gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtactttac
    27901 tgcttatggc actacagcaa cagatggtta ccccactaag cctgaagcga atcgccatca
    27961 attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac
    28021 ttaaagccgg gttaacgctt tctgaagggc tggctctgct ggcggaacag catcccagta
    28081 agcaatggca agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt
    28141 ccaatgcctt attaccctgg tcagaggcat ttccgccgct ctatcaggcg atgatccgca
    28201 cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaat
    28261 cccagcgtca gttgaccgac aaagtgaaat cagcgttgcg ttatcccatc atcattttag
    28321 cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta
    28381 tctataagac cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag
    28441 actttagtgg cgaatggggc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca
    28501 ataagttgct gatgcgccgc ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca
    28561 tcccgattat aggttcactg atgaggggac aaaaactcac gcagatcttt acgattctgg
    28621 cgctgacaca aagtgcaggc attacttttt tacagggcgt agagagcgtc agagaaacaa
    28681 tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc
    28741 atcccatctg gctggcgcta aaaaatgccg gtgagtttag cccgctctgt ttgcaattag
    28801 tgagaacagg agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc
    28861 atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc
    28921 tgatcataac gggaggaatt atcggtacgc tggtggtggc aatgtatctg ccaattttcc
    28981 atttaggcga tgcgatgagt gggatgggat aacgctggcg tgttgctcca cgccagcgtt
    29041 gggagattac aggttgttga agatgcggtt ttcttgttcc agcacacgaa taaacgtggt
    29101 gcgcttggtc agctctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc
    29161 gcccaaaata tcacgcgcgg tattttcaac ctggcctcgc agcggcagct taacggtttt
    29221 accttctgct gcgcggtatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga
    29281 gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct
    29341 ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc
    29401 tttcgccaca tcgcccggcg tggtacagcc accgtcgctg acgatcattc cgcccagacc
    29461 gtgcgccgca tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt
    29521 gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga
    29581 gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt
    29641 cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc
    29701 cacatcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc
    29761 gaaatccgca tcagacgtgc cggtagaaac catcacatgt ttcagcacat cagcggaaga
    29821 attgttgata aacgcttgcc actcttcgac agaaaagtgt ttatgcacag cagtcagaat
    29881 atcaaaagaa gccagcgcag aggccataga aaatgtgcct acggtgtcca tatttgcggc
    29941 gataatcggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag
    30001 ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa
    30061 acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag
    30121 ggtaagagcg caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc
    30181 gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg
    30241 tttcatcaat ttcactttcg cgcgctcatt tttaacgttt tgataatcat gatgaattcc
    30301 cgggaatata aattatgggg tatatagttg ccttaacggg aggcattggc agtggtaaga
    30361 gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg
    30421 cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg
    30481 aaaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg
    30541 ccaacccgga agagaaaaac tggcttaacg acctgctgca tccgctgatt cagcaagaga
    30601 cgcaacacca gatccagcaa gcaacttccc cctatgtact gtgggttgtg ccattgctgg
    30661 tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa
    30721 cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgaacatgtc gaacaaatcc
    30781 ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata
    30841 acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc
    30901 ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca
    30961 tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac
    31021 cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga
    31081 attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttaaccg
    31141 gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga
    31201 agcattaatt cagcagttaa aagcggcggt gagcgtatta atttccgcgc cgcgtatcgg
    31261 gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg
    31321 cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca
    31381 gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac
    31441 catggtgctg gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg
    31501 tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc
    31561 acagctttat ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct
    31621 ggacactgaa aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg
    31681 agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg
    31741 gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg
    31801 agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga
    31861 ctggagcgaa gagccaaagc agtgacattt gtctgatgcc tcacgtaggc ctgataagac
    31921 gcggacagcg tcgcatcagg catcttgtgc aaatgtcgga tgcggcgtga acgccttatc
    31981 cgacctacag acgcttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg
    32041 cattaagacc gatcagcgac atccactcac cgggttgccc ttctttaccc cacggcaccc
    32101 cttcccaact ttcgaccagc cagaaccaca gagttatatt cctgtccggg aattcatatt
    32161 ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc gacctcttcc tgaagttcac
    32221 gcaccaccgc ctgttccggc gtttcgccca tttcaatttt accgccggga aactccagtt
    32281 tattcgccat gtgcgcatct gctgcgcgac gtgttataaa gatttcattg ttctcgttgc
    32341 gaataatacc taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca
    32401 gaatcctgcg ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt
    32461 gtatttttta ccagaaccgc acgggcaagg atcgttacgt cctactttgc gttcaccggt
    32521 ttgcgccgcc agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat
    32581 ttgtgctaaa cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg
    32641 catacgtacc tgaactttgc tcagcgtact gataacttca tatttcaacg actccagcat
    32701 cgctgcaaac atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata
    32761 gccacgcagg tggataccct gacgcagata gtccatcgct gccaggtgct ctttccacag
    32821 ggagtcaagc gtttgcagca tgacgccttt ctcgaagtga cgcatcatct cagcaccaac
    32881 cacttcttct ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt
    32941 ctcttcatgc agttctggtt ctttatccag ccactcggca attggtaaat cgaggtcgaa
    33001 atcgttcttc agacgttcct gcagccccgg aatatcccac atttcttcca gcgactgtgg
    33061 cggaatgtag gcatcaatgg tggctttgaa cacatcttca cgaatgctgt tgatggtttc
    33121 gctcacatcg ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc
    33181 gttagccacg tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac
    33241 tttacgctgg gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctgg
    33301 cttcataccc agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag
    33361 cgcatcttcc atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg
    33421 accgcgcaac tggttatcga tacgacggga ttcgtgacgc tcggtaccga tgatatgcag
    33481 gccacctgct tccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat
    33541 ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc tgccagctac ctccgagcac
    33601 aatatctgta ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc
    33661 ctgagcaaca atcgccgctt cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat
    33721 accggctttg gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac
    33781 cagcaccggc tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt
    33841 ttccgcttca gtcatgtaga ccaggtccgg cagatcttta cgaatcattg gacggttggt
    33901 cggaacaacg acggtatcca gtttgtagat ggagctaaat tcgaaagctt cggtatcagc
    33961 agtaccggtc atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga
    34021 agccagcgtt tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg
    34081 cagaccatcg gaccagcgtc ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat
    34141 aacttcacca tctttaacga tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag
    34201 cgccgccgtt acgtggtgca tcagcatgat gttggccgga gagtacagag actccccttc
    34261 atccatgatg ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag
    34321 gttcacctgg cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga
    34381 gtcttctttt tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc
    34441 cgagctgtct tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat
    34501 ggagtccact tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcagggct
    34561 gaacgccatg ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat
    34621 gtcagctgcg taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccaac
    34681 agtcaggcca aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta
    34741 gtcgttgacg gtaactacgt gcacgccttt accggttagt gcgttcaggt aagcaggcag
    34801 cgttgcggtc agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag
    34861 aaccataccg ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc
    34921 ctcacgcacc acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag
    34981 acgcgcacga aactctgcgg ttttcccttt cagttcttca tcggagagtt tttccatctc
    35041 cggttccatg gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt
    35101 acgactaccg aaaactttag ttaacaattt gattagcata ataaaatctc aaacgccccg
    35161 cgttacggag ttaataaaat gaagtaaagg tttattgttg ttaggtgagg cgttgagggc
    35221 cagcacggat gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg
    35281 taaaatgcgc ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca
    35341 gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag
    35401 caacgggcag tgtttgcggt gccattgcga aagaaagatg acggattacc gtacgaatag
    35461 catgttgatg ccagtaatca acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca
    35521 gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt
    35581 ttgcgggcgc gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcga
    35641 ccatccctaa taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca
    35701 gtattccact cacgttattg ccatcccgat cgaattcaaa cgtatttagc gcacaaatct
    35761 tatcattaaa gcctgaagtc cacagcacga atgaagataa gttgtgcgta aaagtgctta
    35821 agaaaacagc gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg
    35881 ttcacctggc cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg
    35941 tcgttatgcc agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg
    36001 gaaggtcaca tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt
    36061 cagtgcatga ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa
    36121 caagtcaccg atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc
    36181 gtcttcgttc agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag
    36241 gcacaaacca cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg
    36301 gctgatctgg cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga
    36361 atcaatagcc ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt
    36421 aaattcagcc cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt
    36481 tttggcgcag ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc
    36541 gctgccgtcc atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc
    36601 caggcccgcg agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt
    36661 gaccagacac gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac
    36721 cggtggattc aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa
    36781 cgtcagggtg actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt
    36841 aagtgtcctt tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta
    36901 ctatacattc ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt
    36961 cttaatcagc ttgcttacgt aggaatgctg ggatatccag ataatccggc tctttcgcag
    37021 tttgcggcgc attgtcattc actactttag caaccggctt ctgctcctgg gtcagcggag
    37081 ccatcccatg ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca
    37141 gagtgatttc aggacgtttg tccatgccga tacctgtcgc aacaacggtt acgcgcagct
    37201 cgtcattcat atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa
    37261 atgcacggat ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgccg
    37321 tgatgttaac cagcacgccg cgcgcgccag acaggtcgat atcttccagc agcggagaag
    37381 agatagccat ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca
    37441 tcattgcgta gcccatttca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca
    37501 aacccggacg agtaatcagt tcggcgatac cttgcacagc gcctttcagt acatcgttcg
    37561 ctgcgccaaa cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt
    37621 tcgggatagt gatcagagag tccacatgct tggacagttc agtgatcccc tgctcggcga
    37681 atgccatacg cttcttgcct tcaaagttga aaggcttagt gacgacagca acggtcagga
    37741 tacccaaatc ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac
    37801 ccatacccgc agcaataaag accatgtctg caccttccag cgccgcacgc aatgcatcgc
    37861 gatcctcatc agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttgg
    37921 tgataccgct accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat
    37981 cggtatttac cgcgaagaat tcaacacctt caatacgctc gcgcaccatg tgttcaacag
    38041 cattaccgcc gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca
    38101 ttggttcaaa catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc
    38161 ggcctcataa aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc
    38221 caactgatgc tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc
    38281 catagtgaag caatcccacc gccgtcgaat aatacggctc ctgagcataa tccgttaaac
    38341 cggtaatgtt cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg
    38401 ctgcaagacc ttcgatctgc gctgcgccac cggttaatac aatgcctgcc gccaggtgat
    38461 gtttaacccc ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt
    38521 tgagcaactc ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc
    38581 gcggcggacg accgcctacg ctcggcactt ccacgctctc atcttttcca acgatggaac
    38641 ccagcgcaca accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg
    38701 cgtaagcgat atcactggtc acgacattgc cagcataagg aattaccttt gtgtggcgca
    38761 atgccccacc ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga
    38821 cacccagttc acgttcatct tccgtcaata cagaataact tgatgccagt ccggcaaata
    38881 tcagttggtc aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat
    38941 cgttgtgaca tgtgatcagg tgcacttttg cctgcatccg cacacccgaa agtcctaccg
    39001 gattcttgat cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac
    39061 gatgctcatc gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt
    39121 gcgtcacttc ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct
    39181 taccagaaag cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt
    39241 caatggcgcg ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca
    39301 taccacgtga cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt
    39361 cccctactaa agcggcaacc ttcgcggtac caatttccag tcctactacc agttttctgt
    39421 ccgtcgcctt gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc
    39481 tggcggcaag ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct
    39541 aatccgtttg ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg
    39601 aaccaaacgt ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt
    39661 caactgccag gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc
    39721 cagcatctgc cccatttcgc gatagccctg caacacttca ttagcgctgc cttccgggcc
    39781 atacagcatt ggaagcacct gcttgctggt gcgatctggc ggcacgctga aggtgtttcc
    39841 ttccgcgtct accatgtgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg
    39901 aatcttcaat tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg
    39961 ttgttctatt tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc
    40021 caatgccagg atcgactgcc ggatatcgtc attacgcgtg taatggcgtt caccggtcaa
    40081 caccagcttt gagagcggca ggcgttgcgc atcttccatc cagctcaaca cgacccagcc
    40141 gctcaccaac actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc
    40201 attattgcgg cgagaagaaa cctcttcttc gctgtttcgc gtgttcagag cagcctgcga
    40261 catattagtc cgccagttcc agaattcgta ctaccaactg cgagaagctc atacctgcct
    40321 gacgtgccgc catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca
    40381 gcagataaaa ctgtccatca ctgtccagca taacgtcaat acgtccccat cctttgcaac
    40441 ctaacgtcgt ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgcttcca
    40501 gacctgcggg gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga
    40561 aggttccgga cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg
    40621 tgaactccgg cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca
    40681 atcttaatgc atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac
    40741 cttcgcggct cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct
    40801 tatcgctcag gcctttttca aactctgagc gggttaacgc tacccacggc gcgaccggta
    40861 aaccggcacc ttgccacaga agtttgctgc gtagtttatc tattgaaagc gcagatgcca
    40921 tcactccgct gccggtataa ggcaagccca tcagctcgag catgccctgc agcgtaccat
    40981 cttcaccgcc gcgaccgtgt agcgcgataa acactttctg aaaacccatc gacttcagtt
    41041 gcgtcacgtc gacttctttc gggtcgacag gatacgcatc aataccgcct tcacgcagcc
    41101 cggctaacac cgctgcgcca gaattcagag aaacttcccg ctcagcggaa gtcccgccca
    41161 acaggaccgc gattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg
    41221 atttcagcta aagaacgggc aatttttccg atattaccag ccccctgaac gagaatcagg
    41281 tcgttaccgg ttaataccgg tgccagcatc tcggctacct gcgcagggtc cggcaccaga
    41341 atgggatcaa ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc
    41401 ggaattggcg cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc
    41461 agcacattgg cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg cggctgaaac
    41521 agcattacca ggtttttatc cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact
    41581 tccgtcgggt ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact
    41641 ggctccagcg ggaattcacc gaggaaatca aaacggcgac cagtcccctg gaagctctcc
    41701 agcgcacgca aaatagcctc gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct
    41761 gcggcgttca gcgcgttatg acggcctggc gcattcaggg tgacgcgcat cggctctttg
    41821 tcctgacgca gcagcgtaaa gtgcccctgc gggccaatct gctgataatc ttctacacgc
    41881 acgtcggcat cttcgctgaa gccgtaagtt gtggtctgac gtcccacacg cggtaacaat
    41941 tcgcggatca ccggatcatc aacacacatc accgcacgac cgtaaaaagg caggttgtgc
    42001 agaaaattaa taaaggtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg
    42061 tcggcttcga tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca
    42121 ctctcatctg cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc
    42181 gcttttacca gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg
    42241 gaaaccatcg cggtagttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga
    42301 aaacgcatta actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga
    42361 gctgcgacaa tttccgggtt gtcggcagaa atggcgctgg aaacaacgac cacgctggca
    42421 tcacgtacgt tttccgggcg atggttgaaa taaatcgtcg cacccagatt cagtaactgc
    42481 tgcgtgaccg gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga
    42541 acttcggcaa taccgcccat accggcacca ccaatgccga caaaatgtat gtgccgaacg
    42601 cgacgcattt cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac
    42661 gccattaact tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag
    42721 cccggctcac ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc
    42781 gttctgccat ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat
    42841 ccacgctaag ctgtggctgc tcaataattt tggctgcgcc cgctttttcc agcggtagcg
    42901 cattccagta ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg
    42961 ccgcggcgat ttcactcaca gttaaagcac cggagcggca aacgacgaca tccgcccacg
    43021 catacgccgc cgccatatca tcaataaatt cagtcacttt atgctgcggt tgccccgctt
    43081 cggcatacgc ctgttcaacg gattgttgcg aacctttgcc gctctgatgc cagatagtga
    43141 ctgaatcacc cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct
    43201 gggaaccacc cactaccaac acacgaaccg gaccttcacg tccagccaaa cgttgctgcg
    43261 gcaacggcag cgccagcaca tcggtacgca ccgggttacc caccacttcc gcattaggga
    43321 aagcgcctgg aaacgcctgc atcactttgg tagcaatctt cgccagccat ttattggtta
    43381 agcccgcaat accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca
    43441 gaccacctgg acctgataca tagccgccca taccgagtac cacgtcaggt ttgtacgctt
    43501 tcataatcgc ccgcgcctga cgccaggcgt tgaagatacg cagcggcgca gctatcagtg
    43561 cttttatacc ttttccacgc aaaccagaga tacgaatgaa atcaatttcg atgccatgtt
    43621 ttggcactaa gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct
    43681 gagccattag atagtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg
    43741 ccatcaccat taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc
    43801 tttctccaga cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat
    43861 cagtaagctc gaaccaccgt aactgattag cggcaatgtc agacctttgg tcggtaacat
    43921 ccccgccgcc gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca
    43981 ggcgagaaaa ccggaaaaac ggtggtcaat ttctaatgct ttgcggccaa tcgacatcgc
    44041 acgaaaagcg acgaagaata ccattaaaag tgctagtacc acaccgacat aacctaattc
    44101 ttcgccgata atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg
    44161 taccgaatta cctaaacctt gcccccaaag ttcgccgcga ccaaacgcca tcagcgattg
    44221 cgttaactga tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg
    44281 gcggatacgg tacggttcgg cgaggatcag caacacaacc gctgaaatgc ccataccgat
    44341 aatggcaatg aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac
    44401 aaacaacacc accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag
    44461 aatcacgccc atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg
    44521 caccagatag ttggcgatat agcaaaacag cgacagtttt gtcagctccg caggctggat
    44581 acgcagcaaa ccgagatcga tccaacgcga tgccccttta acagcgctac ccactaccag
    44641 cacgatcatc agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa
    44701 ctccatcggc agacgcagcg taatgatcgc caaaataaac gccaaaatca gatagacacc
    44761 atcacgcttc gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc
    44821 cgaggtcacc atgataaagc caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg
    44881 atcgtacatg atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccctttag
    44941 cgccgtggag atccagacca ggatactgaa tcctggcagg cgcggcattt tcaggcgagg
    45001 gagagataaa cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt
    45061 tcaaagttct tgaactgatc aaggctggca caggccgggg agagcagaac catatcgccc
    45121 ggctgaacac gcggagccag caagcgcata gcctgttcca tagtttcggt ttgttctgcc
    45181 acttccgggc gtagcgccgc cagctgcgcg ccgtcacgac cgaaacaata cagacgtacg
    45241 ttatcgccat tcaggtaacg caccagtggg ctaaagtccg ccgatttacc atcgccgccc
    45301 agcaacaaat gcagcgtgcc gtctacgtgc aggccattca gcgccgcttc cgtactgccg
    45361 acgttggtcg cttttgaatc gttaatccag cgcacaccgt tatgctccag caccacttca
    45421 aagcgatgcg gcagaccggt gaaggtggtt aacgctttca ggctgctggc gcgcggtaac
    45481 cctgcggcat ctgccagcgc cagcgccacc agcgcattgg tgtagttatg ctgcccggaa
    45541 agtttcatct ctttcacatt cagcactttc tcgcctttaa cccgcagcca ggtttcgccc
    45601 tgctgatgat tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgttcatcc
    45661 gcaccgcgaa tcggcattgt taaggcatca tcagcattaa ccacgcaaac tttcgcgttt
    45721 tcgtaaatgc gcagttttgc tgcacgatac tgttgtaaac caaacgggta gcgatccata
    45781 tgatcttcag tcacgttcag aatggtcgct gctaccgcct gtaagctgga ggtggtttcc
    45841 agctggaagc tcgacagttc cagcacgtac agttcacact catcatccag tagcatcaac
    45901 gcaggcaggc caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca
    45961 cccactagcg tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct
    46021 tgtgcttcgc gacaaaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca
    46081 gcgcttaagg atggatgcgc cagtgcaata ccgggactgg cgacaatcag atctgccgcc
    46141 atcagccatt catcattcag actgcccgtg tggcgttcta cggcttcggg taatttatcc
    46201 aggccaggcg gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa
    46261 aagtccacgc aggaaagccc ggtgaggccc aggccgataa tgacaacatt tttaccctgg
    46321 taatcagcca tgattaacgt accttcagcg ttgccagacc aatcaggacc agcatcagcg
    46381 aaataatcca gaaacgcaca atgacgcgcg gttccggcca gcctttcagt tcatagtggt
    46441 gatgaatcgg tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca
    46501 ggatgacaga aagcgtttct accacgaaca cgccccccat aatcaccagc aggaattcct
    46561 gacgtagcag tacggcgata atgcctaacg caccacctaa cgccagcgaa ccgacatcgc
    46621 ccataaagac ctgcgccgga taggtgttaa accacaggaa gcccagtcct gccccgacta
    46681 tcgcggtaca gacaataacc agttccccgg cgtgtcgcag atacggtatg tgtaaatagc
    46741 tggcaaagtt catgttgccg gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata
    46801 cggtcggcat aattgccaga ccgtcaagac catcggtcag gtttaccgcg ttgccagtac
    46861 ccacaatgac gaagtaagcc agcagaatgt agaacagccc cagttgcggc atcacatctt
    46921 taaagaatgg gaccaccagc tgcgttgcgg gcgtgtcttt gccggcaagg tacaaagcga
    46981 aagcaacacc cagcgcaatg accgacatcc agaaatattt ccaacgagcg atcaaccctt
    47041 tggtgtcttt acgcaccact ttgcgataat catcaacaaa gccaataaca ccgtaaccta
    47101 ccagcaccac caacacgcac cagacgtacg gattggacgg gtaagcccac agcagcacgg
    47161 agatcacaat cgctgtcagg atcataatcc cgcccatggt cggcgtaccg cgcttgctga
    47221 agtgtgattc aggaccgtcg ttacgcacta cctgaccaaa ggaaagtttt tgcaaatgag
    47281 caatcatgcg cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg
    47341 cgcgaaacgt cagataggaa aagacgttaa agccggaata atatttgacc aaatgttcgg
    47401 ccagccaaac taacatgtcc cattctcctg taaagcgcgt actacctctt ccatggcagc
    47461 actacgtgaa cccttaacta aaatcgtaat tacctgttgc tcagcaatca gtgatttaag
    47521 acgcgtaatt aacgcagttt tatcagcaaa atgttcgcca acgccgctgg cggtgctgat
    47581 agcatggctt tgtttaccca tgcttaacac gcggtcaata ccagcagctt ttgccgcctc
    47641 acccacctgt acatggcagg cttcgctttc agcgcccagt tccgccatat cgcccaccac
    47701 cagcacgcgg tagcccggca tttcagccag tacctgaaca gctgcggtca ttgaaccgac
    47761 attggcgttg taggagtcgt cgagcagcaa ctggttttct gccagttgga tggggaacag
    47821 acggccagga acagctttca gatttgccag ccccgctttg atagcatcaa gcgttgcgcc
    47881 cacggacatg gagagcgcag cggctgccag cgcattcgca atattgtgac gccccggcaa
    47941 cggcagcaga acatcgacgc taccggttgg ggtttgtagg gtaaattcca taccgtgcga
    48001 ggtcacatgg atattggtgg cggtgaaatc gctgttggcg gcattgggtg agaaacgcca
    48061 cactttgcgt gagccaatta cgctctgcca gttcagccag tcgttgttgt cggcgttcat
    48121 aatggcgata ccgttttccg gcaggccgct aaagatttca cctttcgctt tcgcgacacc
    48181 cgcaagcgag ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca gcgccgcttc
    48241 cgggcgagtc agactcacag tccaggcaat ctcgccctga tggttcgcgc caagttcaat
    48301 aactgcgtaa tcgtattccg gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt
    48361 gttgagattg cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat
    48421 ctctttaacg gaggttttgc cggaggaccc cgtcagagca accacgcgcg ccggaacttg
    48481 ctggcgaacc catgcagcca gttcaccaaa cgccagacgc gtatccttga cgattaactg
    48541 cggcaggtcg atgtccagcg gacggctaac cagtagtgcg cctgcgccgc cagctttcgc
    48601 ctggtcggca aaatcgtggg catcaaaacg ttcacctttc agggcaacaa acaggcagcc
    48661 cggcgtcagt tttcgggtat cagtggttac agcatcaagg gtgatatctg caccttgcag
    48721 ttcaccgttg agaatatcgg taagttggct aagggttacg ctaatcatgc aatccccccc
    48781 agcagacgcg ccaccgtgac gcgatcggag tagtccagac gctgattgcc aacaatctgg
    48841 taatcctcat ggcctttgcc cgcgaccagt accacatcat tctctttagc ctgcataacg
    48901 gcgcaagtca ccgcttcagc acggccttcc atcactttgg catgtccggc atctaacatt
    48961 cccgccagaa tatcgttgat gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc
    49021 accaccgcca cgtcagcaaa ctcttcggca attgcgccca tcagtggacg cttaccttta
    49081 tcgcgatcgc caccacagcc aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc
    49141 gcctgtaagg ctttttccag tgcatccggc gtatgcgcgt aatccaccac caccgtcggt
    49201 ttgcctggcg cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt
    49261 ttcagcagat cagccagtgg atagcccagc gccagcagtg ttgccagcgc gagcaacagg
    49321 ttgctgacgt taaaagcgcc catcagatgg ctttcaattt cgccatcgcc ccagcttgaa
    49381 ctaaagcgaa tggtcgcgcc gctgtcgtga tagttcactt cggtcgcttt caaccagcgt
    49441 ccgtgacagt tcggattgat atgatcttcc attgataccg caaccgcgtc cggcagtttt
    49501 gccagccagc ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt
    49561 tcagaataaa gcagccattt cgcagcttcg tagtgttcca tatcaccatg ataatcaagg
    49621 tgatcgcggc ttaagttggt aaagaccgac gccggaaatt tcaatgccgc cacacggtgc
    49681 tgtaccagcc cgtgggagga aacttccatt gcgcaaaaag tcgcgccctg atccaccagc
    49741 cccgccagct catgctgaac atcaactgcc gaaccggtag tattttctgt cgggatcact
    49801 ttccccagca gaccgttacc aacggtgccc attaccgcgc tggtttcgcc aagcagttgg
    49861 ctccactgcg ccagaagctg ggtcgtcgtg gttttgccgt tggtgcccgt tacgcctacg
    49921 agacgtaaat tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg
    49981 ttgagctggc tgagatagat gaccggtacg ccgtgcattt cacggatttc accgtcggtc
    50041 gcctcatctt tcgcctctgc aataatggca gccacacctt gcgctatcgc ctgcgggata
    50101 tatcgacgcc cgtccgcctg atgacctact acagctacaa agagatcgcc cgccgcagcc
    50161 acacggctgt cgagtgtcat ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga
    50221 gcaagaaggt cgcgcaaatt acgatctgcc acctgtcccc tcgccttgat taatcacaaa
    50281 ttcattttta tcgcccgttg tcagcgcatc cggctcgatg ttcatggtac gcaatacgcc
    50341 gcccatgatg gcaccaaaga ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg
    50401 cggatcgttg ataacaacaa ccagcgcgaa gcgcggctga ctcgcaggcg caacgcctgc
    50461 ggtataagca atatatttat tgatgtagcg accgtccggc ccgacctttt tcgcggtacc
    50521 ggttttaatg gcgatacgat agcctttaat cgccgccttc acgccgccgc cgcctggtag
    50581 cgccacgctt tccatcatat gcaccacggt gcgaacaatg gattccggga agacacgttc
    50641 accgggaacc ggggggtcaa ctttggtaat cgacagtggg cgataaatgc cgtagctgcc
    50701 gatagttgcg tagactcgcg ctaactgtaa cggtgttacc attagcccgt agccgaaaga
    50761 gaaggtggcc ctctctatgt cagaccaccg ttgtttttga ggatataagc cactgcgttc
    50821 tccgaccaac cccaaattgg tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa
    50881 cgctgaggac ggcatcgtta acgccagctt ggaaacaccg acgttactcg acttctgtaa
    50941 taccccggtc agggttaatt cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat
    51001 tcgataagga atggtattga gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt
    51061 catcaccacc atcggtttaa ccgttgagcc cggttcaaac acgtcggtga tggtacggtt
    51121 acgcatcgcc tctttcggcg tgccgctcag attgttaggg ttgtatgacg ggctgttagc
    51181 catcgccagc acttcacctg tgttgacatc caccagcacg gcgctaccag attcagcctt
    51241 gttaaaggcc accgcgttgt tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat
    51301 actcagcgcc aggttgtgcg ctgcctggct gtcagtagaa gaaatatctt caattacgcg
    51361 accatagcgg tctttacgca caatgcgctc acccggctgc ccggtaagcc atttatcgaa
    51421 actcttctca acgccctcaa tcccttgact atcgacgtta gtaaagccga tgaggtgagc
    51481 agtcacttcg ccggacggat agtaacggcg agactcttca cgcagatgaa tccccggcag
    51541 tttcagtttt ttgatgtagt ccgccatgtc agagtttacc tgacgcgcca gataaataaa
    51601 gcgccctttc gggttggcgt taatgcgggc agaaagctga tccagcggaa tattgagcgc
    51661 gttagccagc gccttccagc ggtcaccgac gctgataccg ccagcgtcat gcacttcttt
    51721 cgggtcagcc caaatcgctt ttaccggcac gctcaccgct aacgggcgac cagaacggtc
    51781 agtaatcatg ccgcgggagg tggaaacttg ctgaacgcga agagaacgca tgtcgccctc
    51841 tttcaccagc atatccgggg agataacttg taaccacgct acgcgtccga gcagaaaagc
    51901 cagcgccagg agaatacagc cgcataacaa cgcaaaacgc caactgataa agttggcatg
    51961 ttcttcctga cgttttggtt tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc
    52021 ttatttttgc actacgatat tttcttgtga cggatcaaca tgctgcattt gcagcttttc
    52081 cgtggcgatc ctttccaccc ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt
    52141 gcgccattca atgtctaaag catctcgctc cagcaccagt tgttcgcgct gagcggtcag
    52201 taaacgggta tggtgcgccg tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa
    52261 caggcagagt ggcagcttcc caaatcgcaa aagatcgtca ccgataacac caggcaatgc
    52321 atggcgctcg tggcttccca tcgatccttt aactttgctt agagcttctg tcactctgct
    52381 gatcatgcgt tcgtcctctc tgcaatacgc agaactgaac tacgggcacg agggttctca
    52441 gccacctctt cttcgcccgg cattaacttg cctagtgctc gcagctgacg gccacccagt
    52501 tttttgagct gctcttcagt catcggtaac cctgccggaa cttgcggacc gcggctgttt
    52561 tcacgcataa aacgtttcac aatacggtct tccagcgagt ggaagctgat gatcgaaagc
    52621 cgcccacccg gggccagcac gttgagcgag ctttttagcg cctgctctat ctcctccagt
    52681 tcactgttta cccaaatgcg caccgcctgg aaggtacggg tcgcgggatg tttaaactta
    52741 tctttcaccg gcgttgcagc agccacgact tccgccagtt ctttggtgcg ggtcatcggc
    52801 tgttcgcggt tacgctcgac aatggcgcgg gcaatgcgtt tggcaaaatg ctcttcacca
    52861 taggttttca atacccaggc gatatcggct tcttctgcgg tttgtagcca ttcagcggct
    52921 gactgcccac gggttgggtc catacgcatg tccagcggac catcgcgcat aaaggaaaag
    52981 ccgcgttcag catcatcaag ttgcggtgaa gagacgccaa gatcgaggag aatgccgtcg
    53041 atcttgccaa taagatcgcg ctcggcaacg tattcgccca gcgcggagaa aggtccgtgg
    53101 atgatggaga agcgcggatc atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga
    53161 tcgatcgcca gcaaacgccc ctcttcgcca agctgcgaga ggatcagacg tgagtgacca
    53221 ccgcgaccaa aagtcccatc aatgtagatg ccatcaggac ggatattgag gccattaacg
    53281 gcttcatcca gcagcaccgt agtatgttta tagttttcca tcattttata gagacaagtc
    53341 ctgcagtcgc tccgataagt ctccggtagc caactgctct gcgtcgatat cttccttgac
    53401 ctgttgatgc caggttgttt catcccacag ctcaaacttg ttgaactgtc caaccagcat
    53461 cacttctttt gtcagcccgg catgttgccg cagtactggc gcgattaaca atcgacctgc
    53521 gccatccatc tgacattcgc tggcatgacc taacagtagg cgctgcacac ggcgctcaac
    53581 cgggttcatg ctcaacagac gcgataattt ttgctcgata atttcccatt caggcagggg
    53641 gtaaagcagc aggcacgggt gatgaatgtc aatggtgcaa accatttgac cggcagcgtt
    53701 ctcaagcagc tgttcccgct aacgggtagg cactgataag cgccctttgc tgtcgagatt
    53761 gactaacgtt gctccccgga acatgccagc ctcacccctt attcccactt tgccccacaa
    53821 tttcccactg aaaggagttt acggagcgga ggaaaagctt gtcaagccag tcacaaggca
    53881 taccgaggta aaaaacccga tgtttacggc taatatcagc gttgtttatt ttacgtccaa
    53941 cgtgcgaagc aaattaacgt tatgaatagc tgtaagaaaa aatccctcat cgtcatcact
    54001 gttttaaaat gactcactat tttccacagc aaatataaag tgtcagtttg cgacgcgagc
    54061 ggcattttag gacatatctt ccccggttaa cagtccttgt tacgtctgtg tggcgcttga
    54121 tgccagcggg tttgcgcaaa agcaaagaaa agtgtttgtt aaatcggcaa ttcatcgccg
    54181 catgtaacaa aataatgcaa aaacgcgctg tcgatatcac ttaattattg tgaatttcag
    54241 ctggtgagtt aattaacggg cgggtttaaa accatttatc aataatagtc aggaatagtc
    54301 ttatttactt taa
//
LOCUS       DAJVPP010000012        52555 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVPP010000012 DAJVPP010000000
VERSION     DAJVPP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.012"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(12..323)
                     /locus_tag="OQM34_000729"
     CDS             complement(12..323)
                     /locus_tag="OQM34_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS1451289.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="OQM34_000730"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQM34_000730"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="OQM34_000731"
     CDS             702..1055
                     /locus_tag="OQM34_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS1451290.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="OQM34_000732"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="OQM34_000732"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCS1451291.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="OQM34_000733"
     CDS             complement(2811..3281)
                     /locus_tag="OQM34_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCS1451292.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="OQM34_000734"
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="OQM34_000734"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS1451293.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4120..4338)
                     /gene="hha"
                     /locus_tag="OQM34_000735"
     CDS             complement(4120..4338)
                     /gene="hha"
                     /locus_tag="OQM34_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1451294.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="OQM34_000736"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="OQM34_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1451295.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5284..8433)
                     /locus_tag="OQM34_000737"
     CDS             complement(5284..8433)
                     /locus_tag="OQM34_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1451296.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="OQM34_000738"
     CDS             complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="OQM34_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1451297.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9791..10438
                     /gene="acrR"
                     /locus_tag="OQM34_000739"
     CDS             9791..10438
                     /gene="acrR"
                     /locus_tag="OQM34_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1451298.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10566..13928
                     /gene="mscK"
                     /locus_tag="OQM34_000740"
     CDS             10566..13928
                     /gene="mscK"
                     /locus_tag="OQM34_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1451299.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNVMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="OQM34_000741"
     CDS             complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="OQM34_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1451300.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14315..14842)
                     /gene="priC"
                     /locus_tag="OQM34_000742"
     CDS             complement(14315..14842)
                     /gene="priC"
                     /locus_tag="OQM34_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1451301.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14912..15289
                     /locus_tag="OQM34_000743"
     CDS             14912..15289
                     /locus_tag="OQM34_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1451302.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15442..15993
                     /gene="apt"
                     /locus_tag="OQM34_000744"
     CDS             15442..15993
                     /gene="apt"
                     /locus_tag="OQM34_000744"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1451303.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16122..18047
                     /gene="dnaX"
                     /locus_tag="OQM34_000745"
     CDS             16122..18047
                     /gene="dnaX"
                     /locus_tag="OQM34_000745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1451304.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17380..17444
                     /gene="dnaX"
                     /locus_tag="OQM34_000745"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18100..18429
                     /locus_tag="OQM34_000746"
     CDS             18100..18429
                     /locus_tag="OQM34_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1451305.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18429..19034
                     /gene="recR"
                     /locus_tag="OQM34_000747"
     CDS             18429..19034
                     /gene="recR"
                     /locus_tag="OQM34_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1451306.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19144..21018
                     /gene="htpG"
                     /locus_tag="OQM34_000748"
     CDS             19144..21018
                     /gene="htpG"
                     /locus_tag="OQM34_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1451307.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21199..21843
                     /gene="adk"
                     /locus_tag="OQM34_000749"
     CDS             21199..21843
                     /gene="adk"
                     /locus_tag="OQM34_000749"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1451308.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21975..22937
                     /gene="hemH"
                     /locus_tag="OQM34_000750"
     CDS             21975..22937
                     /gene="hemH"
                     /locus_tag="OQM34_000750"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1451309.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22934..23893)
                     /gene="aes"
                     /locus_tag="OQM34_000751"
     CDS             complement(22934..23893)
                     /gene="aes"
                     /locus_tag="OQM34_000751"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCS1451310.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24045..25349
                     /gene="gsk"
                     /locus_tag="OQM34_000752"
     CDS             24045..25349
                     /gene="gsk"
                     /locus_tag="OQM34_000752"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1451311.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="OQM34_000753"
     CDS             complement(25482..27158)
                     /locus_tag="OQM34_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1451312.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="OQM34_000754"
     CDS             complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="OQM34_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1451313.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLMAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /gene="ushA"
                     /locus_tag="OQM34_000755"
     CDS             28834..30486
                     /gene="ushA"
                     /locus_tag="OQM34_000755"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1451314.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="OQM34_000756"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="OQM34_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1451315.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="OQM34_000757"
     CDS             complement(31206..32000)
                     /locus_tag="OQM34_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1451316.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="OQM34_000758"
     CDS             32138..32479
                     /locus_tag="OQM34_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1451317.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="OQM34_000759"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="OQM34_000759"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1451318.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSSSPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="OQM34_000760"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="OQM34_000760"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1451319.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="OQM34_000761"
     CDS             36395..37687
                     /locus_tag="OQM34_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1451320.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALGLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38219
                     /gene="cueR"
                     /locus_tag="OQM34_000762"
     CDS             37812..38219
                     /gene="cueR"
                     /locus_tag="OQM34_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1451321.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38220..38678)
                     /locus_tag="OQM34_000763"
     CDS             complement(38220..38678)
                     /locus_tag="OQM34_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1451322.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="OQM34_000764"
     CDS             complement(38675..39592)
                     /locus_tag="OQM34_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1451323.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="OQM34_000765"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="OQM34_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1451324.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="OQM34_000766"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="OQM34_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1451325.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="OQM34_000767"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="OQM34_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1451326.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="OQM34_000768"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="OQM34_000768"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1451327.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="OQM34_000769"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="OQM34_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="OQM34_000770"
     CDS             43551..44237
                     /locus_tag="OQM34_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1451328.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46643
                     /locus_tag="OQM34_000771"
                     /pseudo
     CDS             44234..46643
                     /locus_tag="OQM34_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(46877..47971)
                     /gene="mnmH"
                     /locus_tag="OQM34_000772"
     CDS             complement(46877..47971)
                     /gene="mnmH"
                     /locus_tag="OQM34_000772"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS1451329.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWFKAQ"
     gene            complement(48040..48966)
                     /gene="allS"
                     /locus_tag="OQM34_000773"
     CDS             complement(48040..48966)
                     /gene="allS"
                     /locus_tag="OQM34_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCS1451330.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49196..49678
                     /gene="allA"
                     /locus_tag="OQM34_000774"
     CDS             49196..49678
                     /gene="allA"
                     /locus_tag="OQM34_000774"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCS1451331.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            49756..50571
                     /gene="allR"
                     /locus_tag="OQM34_000775"
     CDS             49756..50571
                     /gene="allR"
                     /locus_tag="OQM34_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCS1451332.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            50661..52442
                     /gene="gcl"
                     /locus_tag="OQM34_000776"
     CDS             50661..52442
                     /gene="gcl"
                     /locus_tag="OQM34_000776"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCS1451333.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52455..>52555
                     /locus_tag="OQM34_000777"
     CDS             52455..>52555
                     /locus_tag="OQM34_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCS1451334.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVE"
BASE COUNT        12647 a        13540 c        13867 g        12501 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgtcc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aatgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ttggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccag gaagggcagt atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
     2161 caataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctag ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgtgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaattagcga gaatttgctg gcgtaatgtg tgctcttccg ccagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc accgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgcg ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccaaccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctggactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg attacatgtt
     5281 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc ggctaaagcg
     5341 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     5401 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     5461 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
     5521 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     5581 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
     5641 cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     5701 accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc agctctcgta
     5761 cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg aaggtgcctg
     5821 gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
     5881 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     5941 cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac gcggcgaacc
     6001 gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat cagcagcacg
     6061 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     6121 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     6181 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     6241 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
     6301 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
     6361 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
     6421 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     6481 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
     6541 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     6601 ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg ttacctcatt
     6661 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     6721 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     6781 gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca gaataccgcc
     6841 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttcaacc agccgaagaa
     6901 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     6961 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
     7021 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     7081 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     7141 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
     7201 aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca ttgttagcgt
     7261 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     7321 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
     7381 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     7441 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     7501 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
     7561 gatccccaga ccggaagcag gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     7621 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct gattcacttt
     7681 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     7741 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
     7801 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
     7861 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
     7921 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
     7981 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     8041 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
     8101 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     8161 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     8221 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     8281 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
     8341 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     8401 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     8461 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     8521 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     8581 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
     8641 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
     8701 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     8761 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     8821 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     8881 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     8941 gtttcagcgt gccattcgcc agttcctgtt tcaggcgaag gaagtcgttg ctggactggg
     9001 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
     9061 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
     9121 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
     9181 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc ttactgatgt
     9241 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     9301 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
     9361 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
     9421 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
     9481 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     9541 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     9601 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     9661 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     9721 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     9781 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     9841 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
     9901 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     9961 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    10021 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    10081 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    10141 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    10201 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    10261 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    10321 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    10381 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta acgaataacc
    10441 ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct ggttcactgc
    10501 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    10561 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    10621 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    10681 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    10741 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    10801 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    10861 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    10921 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    10981 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    11041 tttcgttaca gacgcagccc gaacgcgtgc aaaatgtgat gtataacgct tcgcagcagc
    11101 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    11161 gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt gaccagcagc
    11221 gtaaaagcct ggaagggaac accgtcttgc aggatacttt gcaaaagcaa cgtgattacg
    11281 tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa gcggtaaaca
    11341 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    11401 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    11461 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    11521 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    11581 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    11641 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    11701 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    11761 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    11821 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    11881 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    11941 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    12001 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    12061 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    12121 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    12181 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    12241 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    12301 tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    12361 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    12421 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    12481 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    12541 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    12601 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc attaccgtgc
    12661 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    12721 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    12781 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    12841 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    12901 ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    12961 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc gtgttcagct
    13021 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    13081 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    13141 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgcc
    13201 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    13261 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    13321 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    13381 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    13441 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    13501 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    13561 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    13621 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    13681 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    13741 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    13801 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    13861 agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg acaccagcgg
    13921 ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    13981 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    14041 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    14101 ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    14161 gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca gcacggtact
    14221 ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    14281 aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc gctagcctgt
    14341 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccactt
    14401 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    14461 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    14521 agcgggaaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gaggcttcac
    14581 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    14641 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg tcgagacaag
    14701 actgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg
    14761 cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    14821 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    14881 gtagccacta tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    14941 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    15001 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    15061 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    15121 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    15181 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    15241 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    15301 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    15361 cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    15421 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    15481 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    15541 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    15601 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    15661 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    15721 gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    15781 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    15841 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    15901 ttcattatca acctgtttga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    15961 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    16021 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    16081 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggttttag
    16141 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    16201 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    16261 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    16321 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    16381 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    16441 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    16501 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    16561 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    16621 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    16681 ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    16741 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    16801 gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
    16861 atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac
    16921 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    16981 gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    17041 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    17101 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    17161 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg
    17221 agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa
    17281 ccctggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    17341 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    17401 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    17461 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    17521 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    17581 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    17641 tggcggtgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca caggtgagcc
    17701 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    17761 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggtgcac
    17821 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    17881 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    17941 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    18001 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    18061 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    18121 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    18181 atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    18241 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    18301 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    18361 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    18421 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    18481 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    18541 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    18601 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    18661 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    18721 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    18781 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    18841 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    18901 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    18961 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc gggcgtcata
    19021 agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa ttattctccc
    19081 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    19141 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    19201 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    19261 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    19321 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    19381 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    19441 tccggtacca aatcattcct cgaatccctg ggttctgacc aagcgaaaga cagccagctg
    19501 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    19561 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    19621 ggtgaatata ccgttgccga tatcaccaaa gaagatcgtg gtactgaaat caccctgcat
    19681 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    19741 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
    19801 accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    19861 gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga ctttaacgat
    19921 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    19981 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    20041 tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa ctatctgcgc
    20101 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    20161 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    20221 ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    20281 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    20341 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    20401 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    20461 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    20521 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    20581 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    20641 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa agctctgctc
    20701 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    20761 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    20821 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    20881 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    20941 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaat
    21001 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    21061 tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    21121 tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa atcgacacat
    21181 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    21241 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    21301 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    21361 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    21421 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    21481 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    21541 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    21601 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    21661 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    21721 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    21781 aaagttgacg gtaccaagca ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    21841 taatcaagcc gcagcagatc tgctttcggg caggtctgct tatccacctc aggcaatacc
    21901 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    21961 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    22021 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    22081 gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc
    22141 tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac ggctcgccgc
    22201 tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgccag
    22261 tggcgctggg catgagctac ggctcgccat cactggaaag ctccgtcgat gaactcctgg
    22321 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    22381 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    22441 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    22501 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    22561 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    22621 gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc tttcagtcgc
    22681 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    22741 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    22801 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    22861 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    22921 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    22981 cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    23041 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    23101 gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    23161 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    23221 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    23281 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    23341 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    23401 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    23461 aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa
    23521 gggtgtaatt aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    23581 gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga gttggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gccaggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaatgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggctgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggttcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc taatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccatca agggtcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgct
    28261 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacctggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gacgacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcacatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttacttacta cggtaattga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atacggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgctg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggaactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca ctggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aatcgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggctatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tgggtttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 tatcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagt acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 ggggcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggta gcgatgaatc attactgtcg cagtcgctac gatctggtgg cgatcctcaa
    45061 aacgctgggg gcagggcgag cgcaactgcg taagctaatc gtcggtcagt ggttgatggt
    45121 gctgacgctt tcagccgtta ccggtggggc cataggcctg ttgttcgaaa acgtgttgat
    45181 ggtgctgctc aagcccgttc tgcctgctgc actaccgcca gccagcctct ggccgtggct
    45241 gtgggcgctt ggcaccatga cggtcatctc gctgctggtg gggctacgac cataccgctt
    45301 gttgctggca acgcagcctt tacgcgtatt acgtaatgat gtggtcgcga acgtctggcc
    45361 gctgaagttt tatctgccga ttgtcagtgt ggtggttgtg ctgctgctcg ccggattaat
    45421 gggtggcagc atgctgcttt gggcggtgct ggcgggcgcg gtagtactgg ctttgctgtg
    45481 cggtgtgctg ggctggatgc tgctgaatgt acttcgccgc atgacgctga aatcgctgcc
    45541 tctgcgcctg gcggttagcc gcctgttacg tcagccgtgg tcaacgttaa gccagctttc
    45601 ggcattttcg ctctccttta tgctgctggc actgctgctg gtgctgcgtg gcgatctgct
    45661 cgaccgctgg cagcagcagc taccgccaga aagcccgaac tactttttaa ttaacatcgc
    45721 cacagaacag gtagcaccgc taaaagcgtt cctcgcggaa catcatatag tcccggaatc
    45781 attttatccg gtagtgcggg cgcggctgac ggcgattaat gataagccga cagaaggtaa
    45841 tgaagatgag gcgcttaatc gcgaactcaa tcttacctgg caaaatacgc ggcccgatca
    45901 taatccgatt gtcgccggta actggccgcc aaaagccgat gaagtgtcga tggaagaggg
    45961 gctggcgaaa cgcttaaacg ttgccctcgg tgataccgtg acttttatgg gcgataccca
    46021 ggagttccgc gctaaagtga ccagcctgcg caaagtggac tgggaaagtc tgcggcctaa
    46081 tttctatttt attttccctg aaggggcatt agacgggcaa ccgcagagct ggcttaccag
    46141 tttccgctgg gagaatggca acggcatgtt gacgcaactc aaccgccagt tcccgaccat
    46201 tagcctgtta gacattggcg cgattttaaa acaggtcggt caggtgctgg agcaggtaag
    46261 tcgggcgctg gaggtgatgg tggtgctggt caccgcctgc ggtatgttgc tgttgctggc
    46321 acaggtgcag gtgggaatgc gccagcgtca tcaggagctg gtggtatggc gcacactcgg
    46381 tgcggggaaa aaactgctgc gcacgacatt gtggtgtgat gttcgccatg cttgggtttg
    46441 tttccggcct ggtggccgca attggtgcgg aaacggcact ggcagtgttg caggcgaaag
    46501 tgtttgattt cccgtgggag ccagactggc gattgtggat tgttctgccg tgcagcggtg
    46561 cgctgctgct gtcgcttttc ggcggctggc tgggtgcgcg actggttaag ggtaaggcgc
    46621 tgttcaggca gtttgtgggg tgatgacagg tgttagattg cctgatgcgc tacgcttatc
    46681 aggcctacga agagcgtgca atatattgat tttgcaagat tgtgtaggcc ggataaggcg
    46741 tttacgccgc atctggcatg aacagtgtgc attttggcaa taaatatgaa accgactgtc
    46801 atgtcggttt tttatatctg tattctggtg caaccatttt gttctcacga caatcgccgt
    46861 ttcagaatac cgcccatcac tgcgccttaa accataccgc cacttccgcc cactcaccgc
    46921 gaaagacaac tttttccgct tttttctcaa gctgatagcg atacattggg tcgtaatatt
    46981 ctttaagtaa cggcaccagc caggccagat gaccgtcggt gctgccggtg gtgagttgcg
    47041 ttgtcagcgc cgtatccagc tgggcagcga gttcgttata gcgctgtaac cccagccgac
    47101 gcttaatcgc cgaaagtccg tgatgcaggt attcgcaata ttcctgccag ccctgttcgt
    47161 cgccataagc gtgggtaaaa tcatgatgca tacgcaagaa atactcttca ttcaggcgtt
    47221 caagacggat ctcaaacgga tcttctacca ccgcaatcgc cgcctgagtc attcgctcgc
    47281 gcagacattc cggcaggtga ttcgaaccga tcatccggct ttcatcttcc agcacccaca
    47341 ggcgcaaatc ctgacgggcg tcggttttta gcatttcggc agccagcagg ttttcaaaac
    47401 tcgcctggct aagttgtggt tgtaacgtgc gaccaaacgc cgaaccgcga tgacgcgcca
    47461 gcccttccag atcaacaccg tttggctgtt gttgcactaa tagcgtttta ccgctgccgg
    47521 tacaaccgcc aatcagcact atcggttttt gtgccagttc aatggtcgcc tgaatcgcgg
    47581 tctggcgcag tgccttataa ccgccttcca ccagcggata atcaatcccc gcttcatgca
    47641 gccagcgttg cacaatatgt gaacgttgac cgccacgggc gcagcagaga ataccttgcg
    47701 gattttgcag gcacgctgcc cgccaggcgt acatacgctg ctgacgaatt tcacccgcca
    47761 ccagtttatg ccccagcgcc agcgctgcgt ctgagccttg ctgtttatag caggtgccaa
    47821 cggaggcgcg ttcatcgtta tccattaacg gcagattgat agcggcgggc attgcgcctt
    47881 gctcaaactc gataggggcg cgaacatcaa taatgggcgt atcagcaatc agcagggcac
    47941 gatagtcctg ttccgtgtgt ctctcttgca tagttaaaag tgaacctcaa atcagcttgc
    48001 gcgctatttt acgcgccaac gcgcaaggaa acttgatttt taactgcgtg gggtgccgaa
    48061 aatttctaaa aatccgctga tttccggcct gtgttgggta aacaaggtca caatatcttc
    48121 taccgctttg cagctgccaa atttgcgcca tgccagactc aatggcgaag gagggcgcat
    48181 cgttgggatt acccggctga ccagttgttg attatcgatc attgactggc aaagtgattt
    48241 tggcaaaaaa ccaatgccaa cacccgccag atgggcggcg attttcgttt ccatatcggg
    48301 aacaataatc tctttttgcc ccggcaatcg ccaggcgacg cgtttggtta aggtgccggc
    48361 gctgtcttca atattgaccg ccggaaagcg acgcaactgc gcttctgtta gcggctcttc
    48421 aacgttcgcc agcggatgat ccgccgccat gacaaatcgc cattgcaccg atcctaaggg
    48481 atcaagacta aaggtatttg ccagcgcctc agttcccgtg accccgatag ccagcgaaaa
    48541 accttcgtac aatagcgagt cccagacgcc catatagatt tgtcgggaga tgtgaaactg
    48601 ggtaaagggg taacgctcat tcagccacgc cagcaactgg gcgacggcct gggggttata
    48661 gagcaggttg ttgatgacaa tattcacctg gcgttccacg ccatcattca cctgttgcag
    48721 ctcgctgggc atactttcca gccagctcag ccagtctctg gcctgggaaa gtagatgctc
    48781 gccagccgct gtcaacgtta cgctacgagt cgtacggaaa aacagcgcta ctccggtatt
    48841 ctcttccaga agtttaatgc gataactgat cgtcgccgtg gttttacata atcgttctgc
    48901 cgcttttgaa aaacttcctg tttcagcaac cgcaatgaaa gtccgcaagg tttctggatc
    48961 gaacatcttc aggtatcccc ttttaaatcc gcaagttgcg tgattttctt atcctctgat
    49021 ttatcagtat ttttacatga taatcctgtt caatttgtgg actaaatcta gttttggaaa
    49081 aacattccaa cttttgtatt gatgctgttc tcttaaggtt ttagattgcc tgttattgaa
    49141 accaagctga ccggtcggcg gtggttgaac ggaattatgt tacaaggaca aaaagatgaa
    49201 acttcaggta ttaccgttaa gtcaggaagc ctttagtgct tatggcgacg taatcgaaac
    49261 gcagcaacgg gattttttcc atattaacaa tggcctggtg gagcgttacc acgatttggc
    49321 gctggttgag attcttgagc aagaccgtac gcttatcagc attaaccgcg cgcaaccggc
    49381 gaatctgccg ctgaccattc acgaactcga acgtcatccg ctgggcactc aggcctttat
    49441 cccaatgaaa ggtgaagtgt ttgtggtggt cgtggcgtta ggcgacgaca aaccagacct
    49501 gtcaacgctg cgggcgttta tcaccaacgg tgaacagggg gtgaattacc atcgtaacgt
    49561 ctggcatcac ccacttttcg cctggcagcg cgtcaccgat tttctgacca tcgatcgcgg
    49621 cggcagtgac aactgtgatg ttgaaagtat tcctgaacag gaactctgtt ttgcgtgacg
    49681 cctgcaaccg acttgcataa gataaactaa ttgttcattg tttatgctca cttgtaggtc
    49741 ggagttaacg taggtatgac ggaagttaga cggcgcggca ggccaggaca ggcggagcct
    49801 gtggcacaga agggcgcaca ggcgttagag cggggaattg cgattctgca atatctggaa
    49861 aaaagtgggg gaagttcgtc ggttagcgat atttctctca atctggattt gccgctctcc
    49921 acgacctttc gcttgctgaa ggttttacaa gcagcggatt ttgtctatca ggacagtcaa
    49981 ttaggctggt ggcatatagg attaggtgtc tttaacgtcg gtgcggcgta catccataac
    50041 cgcgatgtcc tctccgtcgc cgggccgttt atgcgccgcc tgatgttact ttccggcgaa
    50101 acggtcaatg tcgcgatccg taacggcaat gaagcggtat taattggtca gttagagtgt
    50161 aaatcgatgg tcaggatgtg tgcgccactg ggcagtcgtc tgccgctgca tgcttccggt
    50221 gcgggcaaag cgctgcttta tccgctggcg gaagaggagt tgatgagcat cattctgcaa
    50281 accggtttgc agcagtttac gccaactacg cttgtggata tgcccacctt gctgaaggac
    50341 ctggaacaag cgcgtgaact gggctatacc gtagataaag aagagcatgt tgtaggtctg
    50401 aattgcatag cttcagcaat ttacgatgat gtcggtagag ttgttgccgc tatctccatc
    50461 tccgggcctt catcaagact gacagaagat cgttttgtca gtcagggtga gctggtcaga
    50521 gacaccgccc gcgatatcag cacggcgttg ggactgaaag cacatccata gtgtctgtcg
    50581 catcccgctc tgcggagcgg gtttttttga caaaatttga aagttggaaa atttttccaa
    50641 ttaatagagg taggaataaa atggcaaaaa tgagagccgt tgacgcggca atgtatgtgc
    50701 tggagaaaga aggtatcacc accgccttcg gcgttccggg agctgcaatt aatccgttct
    50761 actcagcgat gcgtaagcac ggcggtattc gtcacattct ggcgcgtcat gtggaaggtg
    50821 tttcgcacat ggcggaaggt tatacccgcg caacggcagg gaatatcggc gtatgtctgg
    50881 ggacttccgg tcctgcgggc acggacatga tcaccgcgct ctattccgct tctgctgatt
    50941 ccattcctat tctgtgcatt accggccagg caccgcgcgc ccgtctgcat aaagaagatt
    51001 ttcaggccgt agatattgaa gcaattgcta aaccggtcag caaaatggcg gttacagttc
    51061 gtgaagcggc gctggtgcct cgcgtgctgc aacaggcatt tcacctgatg cgttctggtc
    51121 gtccgggtcc ggtactggtg gatttaccgt tcgacgttca ggttgcggaa atcgagtttg
    51181 atcctgacat gtacgaaccg ctgccggtct acaaacctgc tgccagccgt atgcagatcg
    51241 aaaaagctgt agaaatgtta atccaggccg aacgtccggt gattgttgcc gggggcgggg
    51301 taatcaatgc tgacgcagct gcactgttac aacagtttgc tgaactgacc agcgttccgg
    51361 tgatcccaac gctgatgggc tggggctgta tcccggacga tcatgaactg atggccggga
    51421 tggtcggtct gcaaaccgcg catcgttacg gtaacgcaac gttgctggcg tccgacatgg
    51481 tgtttggtat cggtaaccgt tttgctaacc gtcataccgg ttcggtagag aaatacaccg
    51541 aagggcgcaa aatcgttcat attgatattg agccgacgca aattggtcgc gtgctgtgtc
    51601 cggatctcgg cattgtctct gatgctaaag cggcgctgac actgctggtt gaagtggcgc
    51661 aggagatgca aaaagcgggt cgtctgccgt gtcgtaaaga atgggtcgcc gactgccagc
    51721 agcgtaaacg cactttgctg cgcaaaaccc acttcgacaa cgtgccggtg aaaccgcagc
    51781 gcgtgtatga agagatgaac aaagcctttg gtcgcgatgt ttgttatgtc accactattg
    51841 gtctgtcaca aatcgctgcg gcacaaatgc tgcatgtctt taaagaccgc cactggatca
    51901 actgtggtca ggctggtccg ttaggctgga cgattccggc ggcgctgggg gtttgtgccg
    51961 ctgatccgaa acgcaatgtg gtggcgattt ctggcgactt tgacttccag ttcctgattg
    52021 aagagttagc cgttggcgcg cagttcaaca ttccgtacat ccatgtgctg gtgaataacg
    52081 cctatctcgg cctgattcgt cagtcacaac gcgcttttga catggactac tgcgtgcaac
    52141 tcgctttcga gaatatcaac tccagtgaag tgaatggcta cggtgttgac cacgtaaaag
    52201 tagcggaagg tttaggttgt aaagctattc gggtcttcaa accggaagat attgcgccag
    52261 cctttgaaca ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta
    52321 ttctcgagcg tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg
    52381 aagatatcgc cgataacgca gcggatgcac cgactgaaac ctgcttcatg cactatgaat
    52441 aagggagata aatgatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg
    52501 attttctcgc ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatt
//
LOCUS       DAJVPP010000013        52294 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVPP010000013 DAJVPP010000000
VERSION     DAJVPP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.013"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..338)
                     /gene="yciA"
                     /locus_tag="OQM34_000778"
     CDS             complement(<1..338)
                     /gene="yciA"
                     /locus_tag="OQM34_000778"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1451335.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEA"
     gene            complement(443..982)
                     /locus_tag="OQM34_000779"
     CDS             complement(443..982)
                     /locus_tag="OQM34_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1451336.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(1012..1755)
                     /locus_tag="OQM34_000780"
     CDS             complement(1012..1755)
                     /locus_tag="OQM34_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1451337.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(1783..1872)
                     /locus_tag="OQM34_000781"
                     /pseudo
     CDS             complement(1783..1872)
                     /locus_tag="OQM34_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2112..2750
                     /gene="ompW"
                     /locus_tag="OQM34_000782"
     CDS             2112..2750
                     /gene="ompW"
                     /locus_tag="OQM34_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1451338.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(2810..3316)
                     /locus_tag="OQM34_000783"
     CDS             complement(2810..3316)
                     /locus_tag="OQM34_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1451339.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(3362..3862)
                     /locus_tag="OQM34_000784"
     CDS             complement(3362..3862)
                     /locus_tag="OQM34_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1451340.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(3948..4127)
                     /locus_tag="OQM34_000785"
     CDS             complement(3948..4127)
                     /locus_tag="OQM34_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCS1451341.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(4508..5314)
                     /gene="trpA"
                     /locus_tag="OQM34_000786"
     CDS             complement(4508..5314)
                     /gene="trpA"
                     /locus_tag="OQM34_000786"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1451342.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(5314..6507)
                     /gene="trpB"
                     /locus_tag="OQM34_000787"
     CDS             complement(5314..6507)
                     /gene="trpB"
                     /locus_tag="OQM34_000787"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1451343.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(6519..7880)
                     /gene="trpCF"
                     /locus_tag="OQM34_000788"
     CDS             complement(6519..7880)
                     /gene="trpCF"
                     /locus_tag="OQM34_000788"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1451344.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQLGIKDARLLASVFQTL
                     RAY"
     gene            complement(7881..9476)
                     /gene="trpD"
                     /locus_tag="OQM34_000789"
     CDS             complement(7881..9476)
                     /gene="trpD"
                     /locus_tag="OQM34_000789"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1451345.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(9476..11038)
                     /gene="trpE"
                     /locus_tag="OQM34_000790"
     CDS             complement(9476..11038)
                     /gene="trpE"
                     /locus_tag="OQM34_000790"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1451346.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLC
                     AIATAHHAQETF"
     misc_feature    complement(11060..11153)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(11130..11174)
                     /gene="trpL"
                     /locus_tag="OQM34_000791"
     CDS             complement(11130..11174)
                     /gene="trpL"
                     /locus_tag="OQM34_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS1451347.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            11312..12193
                     /gene="yciV"
                     /locus_tag="OQM34_000792"
     CDS             11312..12193
                     /gene="yciV"
                     /locus_tag="OQM34_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1451348.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            12190..12810
                     /locus_tag="OQM34_000793"
     CDS             12190..12810
                     /locus_tag="OQM34_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1451349.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            12911..13783
                     /gene="rluB"
                     /locus_tag="OQM34_000794"
     CDS             12911..13783
                     /gene="rluB"
                     /locus_tag="OQM34_000794"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1451350.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(13823..14413)
                     /gene="cobO"
                     /locus_tag="OQM34_000795"
     CDS             complement(13823..14413)
                     /gene="cobO"
                     /locus_tag="OQM34_000795"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCS1451351.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(14410..15168)
                     /locus_tag="OQM34_000796"
     CDS             complement(14410..15168)
                     /locus_tag="OQM34_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1451352.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            15388..16437
                     /gene="sohB"
                     /locus_tag="OQM34_000797"
     CDS             15388..16437
                     /gene="sohB"
                     /locus_tag="OQM34_000797"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1451353.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(16473..16724)
                     /locus_tag="OQM34_000798"
     CDS             complement(16473..16724)
                     /locus_tag="OQM34_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1451354.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            17104..19701
                     /gene="topA"
                     /locus_tag="OQM34_000799"
     CDS             17104..19701
                     /gene="topA"
                     /locus_tag="OQM34_000799"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1451355.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            19911..20885
                     /gene="cysB"
                     /locus_tag="OQM34_000800"
     CDS             19911..20885
                     /gene="cysB"
                     /locus_tag="OQM34_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1451356.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            21180..21344
                     /locus_tag="OQM34_000801"
     CDS             21180..21344
                     /locus_tag="OQM34_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1451357.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            21347..21514
                     /locus_tag="OQM34_000802"
     CDS             21347..21514
                     /locus_tag="OQM34_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451358.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            21628..21723
                     /locus_tag="OQM34_000803"
     CDS             21628..21723
                     /locus_tag="OQM34_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451359.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            21886..24561
                     /gene="acnA"
                     /locus_tag="OQM34_000804"
     CDS             21886..24561
                     /gene="acnA"
                     /locus_tag="OQM34_000804"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCS1451360.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTIVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(24625..25215)
                     /gene="ribA"
                     /locus_tag="OQM34_000805"
     CDS             complement(24625..25215)
                     /gene="ribA"
                     /locus_tag="OQM34_000805"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1451361.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            25385..26149
                     /gene="pgpB"
                     /locus_tag="OQM34_000806"
     CDS             25385..26149
                     /gene="pgpB"
                     /locus_tag="OQM34_000806"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1451362.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            26298..26606
                     /gene="lapA"
                     /locus_tag="OQM34_000807"
     CDS             26298..26606
                     /gene="lapA"
                     /locus_tag="OQM34_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1451363.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            26613..27782
                     /gene="lapB"
                     /locus_tag="OQM34_000808"
     CDS             26613..27782
                     /gene="lapB"
                     /locus_tag="OQM34_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1451364.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            27975..28712
                     /gene="pyrF"
                     /locus_tag="OQM34_000809"
     CDS             27975..28712
                     /gene="pyrF"
                     /locus_tag="OQM34_000809"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1451365.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            28712..29038
                     /gene="yciH"
                     /locus_tag="OQM34_000810"
     CDS             28712..29038
                     /gene="yciH"
                     /locus_tag="OQM34_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1451366.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(29164..29382)
                     /gene="osmB"
                     /locus_tag="OQM34_000811"
     CDS             complement(29164..29382)
                     /gene="osmB"
                     /locus_tag="OQM34_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1451367.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(29651..30400)
                     /locus_tag="OQM34_000812"
     CDS             complement(29651..30400)
                     /locus_tag="OQM34_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1451368.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(30486..30659)
                     /locus_tag="OQM34_000813"
     CDS             complement(30486..30659)
                     /locus_tag="OQM34_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451369.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(30807..32781)
                     /gene="pdeR"
                     /locus_tag="OQM34_000814"
                     /pseudo
     CDS             complement(30807..32781)
                     /gene="pdeR"
                     /locus_tag="OQM34_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            32835..32937
                     /locus_tag="OQM34_000815"
                     /pseudo
     CDS             32835..32937
                     /locus_tag="OQM34_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33015..34949)
                     /gene="rnb"
                     /locus_tag="OQM34_000816"
     CDS             complement(33015..34949)
                     /gene="rnb"
                     /locus_tag="OQM34_000816"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1451370.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(35017..36144)
                     /locus_tag="OQM34_000817"
     CDS             complement(35017..36144)
                     /locus_tag="OQM34_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCS1451371.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(36288..37076)
                     /gene="fabI"
                     /locus_tag="OQM34_000818"
     CDS             complement(36288..37076)
                     /gene="fabI"
                     /locus_tag="OQM34_000818"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1451372.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(37443..37796)
                     /locus_tag="OQM34_000819"
     CDS             complement(37443..37796)
                     /locus_tag="OQM34_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1451373.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(37864..38670)
                     /gene="sapF"
                     /locus_tag="OQM34_000820"
     CDS             complement(37864..38670)
                     /gene="sapF"
                     /locus_tag="OQM34_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1451374.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(38672..39664)
                     /gene="sapD"
                     /locus_tag="OQM34_000821"
     CDS             complement(38672..39664)
                     /gene="sapD"
                     /locus_tag="OQM34_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1451375.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            complement(39664..40554)
                     /gene="sapC"
                     /locus_tag="OQM34_000822"
     CDS             complement(39664..40554)
                     /gene="sapC"
                     /locus_tag="OQM34_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1451376.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(40541..41506)
                     /gene="sapB"
                     /locus_tag="OQM34_000823"
     CDS             complement(40541..41506)
                     /gene="sapB"
                     /locus_tag="OQM34_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1451377.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(41503..43146)
                     /gene="sapA"
                     /locus_tag="OQM34_000824"
     CDS             complement(41503..43146)
                     /gene="sapA"
                     /locus_tag="OQM34_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1451378.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(43459..43704)
                     /locus_tag="OQM34_000825"
     CDS             complement(43459..43704)
                     /locus_tag="OQM34_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1451379.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(43838..45223)
                     /gene="puuP"
                     /locus_tag="OQM34_000826"
     CDS             complement(43838..45223)
                     /gene="puuP"
                     /locus_tag="OQM34_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1451380.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(45213..45377)
                     /locus_tag="OQM34_000827"
     CDS             complement(45213..45377)
                     /locus_tag="OQM34_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451381.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(45526..46944)
                     /gene="puuA"
                     /locus_tag="OQM34_000828"
     CDS             complement(45526..46944)
                     /gene="puuA"
                     /locus_tag="OQM34_000828"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1451382.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            47156..47920
                     /gene="puuD"
                     /locus_tag="OQM34_000829"
     CDS             47156..47920
                     /gene="puuD"
                     /locus_tag="OQM34_000829"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCS1451383.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            47947..48504
                     /gene="puuR"
                     /locus_tag="OQM34_000830"
     CDS             47947..48504
                     /gene="puuR"
                     /locus_tag="OQM34_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1451384.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            48654..50141
                     /gene="puuC"
                     /locus_tag="OQM34_000831"
                     /pseudo
     CDS             48654..50141
                     /gene="puuC"
                     /locus_tag="OQM34_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            50143..51423
                     /gene="puuB"
                     /locus_tag="OQM34_000832"
     CDS             50143..51423
                     /gene="puuB"
                     /locus_tag="OQM34_000832"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCS1451385.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            51461..>52294
                     /locus_tag="OQM34_000833"
     CDS             51461..>52294
                     /locus_tag="OQM34_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1451386.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GV"
BASE COUNT        13075 a        13124 c        13558 g        12537 t
ORIGIN      
        1 gcttctgtcg ctttatagcg ttgcccgatt ggttcagacg ctactttttt cacccatact
       61 tcaatattaa tgctgaccga tgtcgtccct ttctggacac agcgtgcata gcagcacacc
      121 acatcgccga ccgcaaccgg ccgtaagaaa gtcattcctt caacccgcac agtcactacg
      181 cgaccgtggg caatttcttt tgccagaata gcgccgccaa tatccatttg tgacattaac
      241 caaccaccaa agatgtcacc attggcattg gtatcggcgg gcatggctaa agtacgtaaa
      301 acaagatcgc cctgagggac gttatgtgtt gtagacatgg taaaaccgac ttaaaaggaa
      361 aatcacaggc gcgatgctac tatgattttc ctgaggagaa cagaggagat acagcgccag
      421 ccccgaagga ctggcagtct ggttaggatt tatcttcctg cggcatgtgg cggtagatat
      481 agataccgct taacaatgtg aagattaagg taagggcggt caggccaaag actttaaagt
      541 tgacccaaat attttgcggc agccagaatg cgatgtagat gtttgccaga ccgcaaagga
      601 taaagaaaac agcccaggcc agatttagct tcgaccatac cggttgcggc agcgtgagtt
      661 ctttactcag catccgctga attagcggct ttttcatcac ccattggctg actaacaggg
      721 ctcccgcaaa cagagcataa atgactgtaa ccttccattt aataaactca tcattgtgga
      781 agaacagcgt caagccaccg aagacgacca ccagaacaaa agtgatcagg gccatcttct
      841 caaccttacg aaagcgaacc cagctataga taagcacaat cgccgtggcg acgatcagcg
      901 ctgcagtagc cgcatagatg tcataaatct tgtaaaacgc gaaaaagaca accagcggta
      961 aaaaatcaag aaactgcttc attttacgat tccgtaatca aatgcttaag gttattggcg
     1021 aatcaacata tacaggcgga acagatagat gagcaatatg gcagaaatca ggttgctcaa
     1081 ggtgttcgcc agtacggcac caatttccgg ggttaatgcg gcaaaagaag aggcaaaaag
     1141 cagcaacagt gtttttgcca gcaaccagct cagtactgcg ggtgccacca gacgcatatt
     1201 cgcccaagtc agccgcatac tgctacgcat cgaggcaaaa atgcccattt tgtcctgtac
     1261 cagcatcacc ggtgccaggg ccagtaaaat ggccatgata attcccggaa cgaccaccag
     1321 catgatgcca atctgtacta aaagggtagt cagaaaaatc agaataaata acttgggcaa
     1381 tatcggcgca ctggcaccaa tagctcgcag cgcactgact ctctgacccg cagacaccag
     1441 ctggataatt aatattacgc ctccggcgag aatggcgtta ccgattaatt ctgaaaaagt
     1501 ggacgccgct gaagcctgca gcaaaatttg ttgctgttcc ggtgacatat tctgaaccag
     1561 gtcgaacaac ccactactgc cgctaacggg cacgccgtca ttgagctgcg caagctgtgc
     1621 atcactgggt gagaaaacat gccctaacac cactgtgata aacgcacata gcaacgatac
     1681 caacagaatg gtcataaatt gattacggaa gaaatttccg gtgtcacggt atacggactg
     1741 cgccgtgata gacatgcact ctccttgagt tttatgcagg tgttaattag cgggcaattg
     1801 taccctggtt aagcctcggg tggcagcatc aggtcttgta tggaaaagca tatctttgta
     1861 aagcggaggt aattgctggc cttcaggcgt gggcaaaccg cggcaagtgg cgatcgagtt
     1921 aatatttgcg tagagaaaat atttaaaaat tgatttaaat cacattaacc aggattctca
     1981 atgcaacttc taaattaatc cagatcaata aatggtgaat tatcatatgt aatgtgatct
     2041 atgtaggatc atttgttact ccaatgtagg tatattcgtc acgtttttat aaccataacg
     2101 acggagcgga tatgaaaaag ttaacagtgg cggctttggc agtaacaact cttctttctg
     2161 gcagtgcctt tgcgcatgaa gcaggcgaat tttttatgcg tgcaggttct gcaaccgtac
     2221 gtccaacaga aggtgctggt ggtacgttag gaagtctggg tggattcagc gtgaccaata
     2281 acactcaact gggcttgacg tttacttata tggcgaccga caacattggt gtggaattac
     2341 tggcagcgac gccgttccgc cataaaatcg gcacccgggc gaccggcgat attgcaaccg
     2401 ttcaccatct gccgccaaca ctgatggcgc agtggtattt tggtgatgcc agcagcaaat
     2461 tccgtcctta cgttggggcc ggtattaact acaccacctt ctttgataat ggatttaacg
     2521 atcatggcaa agaggcgggg ctttccgatc tcagtctgaa agattcctgg ggagctgccg
     2581 ggcaggtggg ggttgattat ctgattaacc gtgactggtt ggttaacatg tcagtgtggt
     2641 acatggatat cgataccacc gccaattata agctaggcgg tgcacagcaa cacgatagcg
     2701 tacgcctcga tccgtgggtg tttatgttct cagcaggata tcgtttttaa ttccgcacaa
     2761 aaacgacccc gtaatatacg gggtcaataa ggacatggta taaagggtat tatttcttcg
     2821 cttctacgcc atcagtttca gaacgaatta aaaatttctc agttgtttgc ggaatatgct
     2881 gaatcagcca gtcggccatt tgcttttcct catttaaaat cgcttcgatg atcggaattg
     2941 agactgtatc accggcattt tttgctgctg ctaatagtga ggtgtaacag gtgatttcaa
     3001 attgctcgaa gacatatccg ccaatagagc ctttaactat ttcatcagaa gggaatatac
     3061 caccgattga ctgcccaagc gcagccattt tactcatgga atctttaatg actgaacgtg
     3121 aaatgtcatt acgatcaaga atagtttcca gttgaacaat ctggtttttg gtttcactaa
     3181 gatgttgttc aatacgagcg cgtagttcag gataattatc tatacggctg gccatggatt
     3241 caagcataga ttcggcttgc ttttccattg cgtgggcgtc acgtaaccag tcatgataat
     3301 gttcaatacg attcatgaaa atgtcctctt tataaaatga ctaaaagtta aaattcatat
     3361 ttcaggcttt attttcggct ttcttattta cgttattaag ggccagatca gtcagtttga
     3421 tgtcggtggc cttttcttct tccagggttt ctttcagaag cttcgctgct ttacggtaac
     3481 ctaattgttc agccagcgtc gctaatgtcc cgtaactggc aatctcataa tgctcgactt
     3541 tctgtgctgc ggcaatcagt gcggcatcac gcacttcgtt tttctcggta ctttcgatga
     3601 cctcattagc ttcttcaata agaccttcca ttgccacaca tttcatgcgc ttaattttca
     3661 gatttgattc tgattccaca acttgatcaa ttcgttcaat ctgtccatga gtttcctcga
     3721 ggtgcgcatg aaaagcctga cttaattttt catttgatgt tgctcttgcg agttttgcca
     3781 gtgcccgggt taattgtttt tctgcgctgt aggtatctga aagcagttga ataaatacat
     3841 cttcaatggt cttcatattc atatttttct ccagtgaaat cactgcgagc catacggccc
     3901 gcagatgcaa ttatctttat cagctgatat taataattaa cggtgaatca ggatttgcct
     3961 gatttattac caccgctttg ttgaccgcct tttttacccg cttcagatgc gcgttgcgga
     4021 tcatttttaa aattaccgcc gctatgctga ccgcctttac ggcctgcgtc ggatgccttc
     4081 tcacggtctt cggcgaaatt tcctgaacta ccacgatgtt cggccatgtt atttctcccg
     4141 ttgcgttgca ttgtttcatt aatatgagtg ttgtgtgtcg acactcatta aaattagtcg
     4201 ctaatgagaa ttagtcaaat taagcgcaac gagaagatag agggaaaata tattttgagg
     4261 aacattctgg atatattaac aattacctga ggaatatgtg acttagagaa aattattgat
     4321 tttactggtg ttatgttgcg ggattaattt gtttgattat attactgttg ggcggaaaaa
     4381 tgacgtaagt tgacgttcga ccggggtaag cgaaacggta aaaagataaa tattaaatga
     4441 atttaggatt tttccggctt cattaaagaa agttaaaatg ccgccagcgg aactggcggc
     4501 tgtgggatta actgcgcgtc gccgctttca tcggttgtac aaaaactttc agtgccgcca
     4561 gcattttctc tggctcatta atatgttgct cgatgatttt aacaatggcc gaaccagaaa
     4621 tcgcgcccgc agctcctgca tcaatcgctg cttttacctg atccggggcg gaaataccaa
     4681 acccctgcaa tggaggtgca gcgttgtact ctttcagctt cgcaaccaga tgattgaggg
     4741 gtaacgcggc gcggttttct gcgccggtca cgcccgctcg tgacagcaaa tatgtgtaac
     4801 cacgaccgta agaggctatc tggcgcagca ggtcgtcatc ggcatttggc gggcagatga
     4861 agataggtgc gacattatgg cgcaacgcgg cctggcggaa gggcgcggac tcttcaactg
     4921 gcacatcggc aaccagcacc gaatcgacgc cgactttctc gcactgggca taaaactcat
     4981 caatgccttt gttaaacacc agattggcat acatcaacag gccaatggga atggtcgggt
     5041 gtttctggcg aatcagtgcc agcatttcaa aacattgtgc cggagtcaca cctgccgcaa
     5101 aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc cagtgggtcg gagaagggga
     5161 tacctaactc cagcgcgtca gcaccggctt caattagcgt atcgataatt ttcaatgact
     5221 gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa tgcgccttct ttgcgctcct
     5281 tcaactgggc aaacagagat tcgtagcgtt ccatcagatt tcccctcgtg ctttcaaaat
     5341 atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa aggttaacca ccagtagctg
     5401 ctctttatcc gggttttcgc gcatcatttt cagcgcatgg gccagggcgt gggaggattc
     5461 cagcgccggg atgatccctt cgtgcaggca cagcgttttg aaggcttcaa gggcttcatc
     5521 atcggtaata gacacgtaat cagcgcgtcc agtgctgtta agatacgcgt gttgtggacc
     5581 gacagacggg aaatccagtc cggcggagat ggagtaagat tcttcaatct gcccgtcttc
     5641 ggtttgcatc atcggcgctt tcataccgaa ataaatgccc acgcgaccat gttttagcgg
     5701 tgcgccgtgc tcgccagttt cgataccgtg accaccaggc tccacaccaa tcaggccgac
     5761 gttggtttca ttgatgaaat cagcaaacat gccgatggca ttcgaaccgc cgccaacaca
     5821 ggcgataacg gcatccggca ggcgaccttc tctttccaga atctgcgctt tggtttcttc
     5881 gccaatcatc cgctgaaact cacggacaat ggtcggataa ggatgcgggc cagccgcggt
     5941 gcccagcata tagtgcgcgg tttcataact gccggaccag tcgcgtagcg cctcattaca
     6001 ggcatctttc agcgtggcgg aaccgctatg taccgggatc acttccgcac ccattaagcg
     6061 catacgaaaa acgttaggcg actggcgttc aacgtctttg gcacccatat aaatacggca
     6121 tttcaggccg agcagggcgc tggcaagggc cgacgccacg ccatgctgac cggccccggt
     6181 ttcggcgatg atttcggttt tacccatccg cttcgccagc aacgcctgcc ccagcacctg
     6241 gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc ttgagataca gcgtggtgtt
     6301 cgtcccggct gtaatgttct ggcatttggt cagtgcggtt ggacgcccgg catagttttt
     6361 cagcaggtcg ttgaactggg cttgaaattc aggatctttt tgcgcgctga caaaagcttc
     6421 ttccagctgg cgcagagcag gcatcaggat ttgtggcacg tacatgccgc caaactcacc
     6481 aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt aatatgcgcg cagcgtctgg
     6541 aaaaccgagg ccaaaagacg tgcgtctttg atgcccagtt gcgactctac agcagaatta
     6601 aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc agttatctgc gcctaagccc
     6661 cccgccagca gaacgttgcc aagcgattga ccatttaata gtgaccagtc aaaacgttgc
     6721 ccgcttccac cctggccgtt gtctaaaaca tatttatcaa cgtgctgaaa ctcgcgggcg
     6781 ggcagggttt caccgacgct taatgctttc cagatggcaa catgtgctgg cagagcttca
     6841 cgcagcgtat cgatatacag ctgatcttca ttaccatgca gttgcactgc cgccagcgat
     6901 aacaccttag ctttgtccac cacatcggca atatcgtgat tgcggaacac gccaacatac
     6961 tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa cgttgacgca acgcggtgat
     7021 gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt cataagctgc tttagcatct
     7081 tgcccacgcg tcaggccaca tactttattc tcacccagca acacccggcg cacggcggcg
     7141 tgcaaatcgt catgggccat caacgccgaa ccaatcagaa aaccgttagc gaagtggctt
     7201 aactcgcgca cctgagcgta agtattgatg ccggattcgc tgattaccgt tacgttgtgc
     7261 cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa tcgacaaatc acgcagatcg
     7321 cggttgttga tgccaacgac ctttgcccct aatgcaatgg cgcgctccag ttcctcttca
     7381 ttactgactt cggtcagcac ccccatctcc agactgtgag cgacggctgc aagctggcga
     7441 tattggtcgt catccagtac tgaaagcatt aataagcagg catcggcctg gtaatagcgc
     7501 gccagataga tctggtaagg gtcgataatg aagtctttac ataaaatcgg ctgcggggcg
     7561 atttggctga cgatgaggag gaaatcaaag ctcccctgaa aatatttctc atcagtcagc
     7621 accgaaattg ccgaagcgta atgtttataa atggcggcaa tgcgtgccgg atcgaaatca
     7681 tcacggatca cgccttttga cggcgacgct tttttacact ccagaataaa cgccgtgcgt
     7741 gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct gaacctcatt ctgaaaactg
     7801 gccagcggtt gctgctgttt gcgggtttct acccaaatcg ccttgtctgc gacgattttc
     7861 gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg cggtaactct gtcgtaagcg
     7921 gaaccactgc gcagtacctc aagaacggtt tgcgcattgg cttgcagatc ttcatggcca
     7981 tgcaggcgca ttaacatggc gacgttcgca gcgacggctg cttcatgggc ggcgtcgcct
     8041 ttaccttgta acaagcgtgt taaaatgtca cggttttctt caggtgttcc gcctgccagt
     8101 tgctcctggt ggtagggagt caggccaaaa tcttcagcgg tcaattgata gctcttaatt
     8161 ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta atgaaacttc atccatcccg
     8221 ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc gcaaggtttc ggcaatcggc
     8281 agcaccagtt ccggactata aacaccaatt aacgccagcg gcggatgcgc cgggttaatc
     8341 aatggcccca gcacattgaa cagggtgcgg gttttcagtt gctggcgaac cggcatcgcg
     8401 tggcggaatc cggtgtgata tttcggcgca aagaggaaac atacgcctaa ctcgtccagc
     8461 gcctggcgcg atttattggc gttcatatca agattaatac cgaatgccgc cagcagatcc
     8521 gacgagccgg atttactgga gacgctgcgg ttaccgtgtt tcgccacttt cagcccacag
     8581 gccgcggcga caaacgcact ggcggtagaa atattgatac tgttgctgcc gtcaccgcca
     8641 gtaccgacga tatcagcaaa cagataatcc gggcgcggga acggcgctgc gttttccagt
     8701 agcgcggttg ctgccccggc gatctcgttc gggtgctcac cgcgaatttt catgctcacc
     8761 agcgccgccg ccagttgttc cggcttcagc tcgccacgca ccaccgctga aaacagctgg
     8821 tggctttctt gttggctaag cgtctgcgcc tgatacagtt tttccagaat cggttgcagc
     8881 gtgttggctg gctctagttt ctgctgcgcc caggccagcg tttgttccag caggcgagcg
     8941 ccctgggtgg tgagaatgga ttccggatgg aactggaatc cacaaacgcg atccgcatcg
     9001 tgacgtactg ccatcaccat gccattaaaa tgggcgttga tggttaaacc ggccggaatg
     9061 ttactgccaa ccagcgagtg ataacgcgcc accggcagcg ggtttgttaa tccggcaaac
     9121 atcgcctgac cgtcatgttc aatgctcgac gctttaccgt gaagaatttc gcccgcctga
     9181 ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc cgaggcaaat gccaataatg
     9241 ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac aaccggcttc gctcggcaca
     9301 ccggggccag gagaaagcat cagtaccgga ttgctcatcg tcgccaggcg ttcaattaag
     9361 gtctgcgccg gaatatggtt gcggtaaatc accacgttat gaccattgct gcgcaactga
     9421 tctgccaggt tgtacgtaaa agagtcgata ttatcgagca gcagaatgtc agccatcaga
     9481 aagtctcctg tgcatgatgc gcggtggcaa tagcgcacag tacagcgcgg gctttgttac
     9541 gggtttcgtc ggcttccgac tgcggaacag aatcaaggac tacaccagca cccgcttgca
     9601 cggtggcgat accgttttcc accagcgccg agcggatcac aatgcaggtg tcgagatcgc
     9661 catgcgcggt gaaataacct accgcgccgc cgtagctgcc gcggcgacga ccttccgcct
     9721 cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct taacgtcccc atattcatac
     9781 aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag ttcgccgact acgcgagaga
     9841 cgaggtgcat cacataggaa taacggtcaa ctttggtgag atcggcgatg tagcggctgc
     9901 caggggtgca aatgcgtgcc agatcattac gggcgagatc aaccagcatc agatgttcag
     9961 acagctcttt atggtcagta cgcatttcca gttcaatacg gctgtcgaga tctctgtcca
    10021 gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc aatcgggtag atctcaatct
    10081 ggcggctggt ggcatcatac ttgagcgagc tttccggcga cgcgccaaat agggtgaaat
    10141 cattatcctg cataaaaaac atgtacgggc tgggattact ctttttcagc acgtaatagg
    10201 ccgccagcgg tgacgggcag ggcagagaga aacggcgaga tggcaccacc tggaaaattt
    10261 ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc accgaactct tcatcgctct
    10321 gattacattc acaacgcata tgcggcacgg aaaccactgg cagcggcggc gcggcttcgg
    10381 tcagttgctg acgtagttcg ttcaggcgag cagtgagacg ttgtttttct tcttcattcg
    10441 gagcaaacag gctggcctga atacgggtgc tttttttctg atggtcaatc accatcagcg
    10501 tttcagcgag ataaaaacag aaatcagggc agttattttc cgctgacagt tgcggtaaat
    10561 cttcaaatcc cgccacaagg tcataagaga acaggccgcc gaagaacatt gcttctcgtt
    10621 cttccttcgg tacattcaac agattctgca ataaacggaa agcgtcaaaa accgaaaggg
    10681 agcataagcg ggcgtcttca tccagcagtg gactgacagg ggggaagcgc agcacacggc
    10741 agtttggtga ttgttcactt tccacacccg caggcagggc gttatccagt agtgccagca
    10801 gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt gtcacctaat gctgtaatgc
    10861 gcagcgcact gtctatcagc agcaggcttt ttaaatcatc tttgctgtcg atatctgcgg
    10921 attccagcag cagcgttgcc ggacgattcc cacacaactg gtgaaaaagc gcagtcgggt
    10981 tgtcgcgata agcgccttcg caggttagca gttcgagagt cggtttttgt gtttgcattg
    11041 ttattctcta attttgttca aaaaaaagcc cgctcattag gcgggctggg tatctgattg
    11101 ctttacgcat ggtgaataca ctgcccgttt caggaagtgc gccaccaacc tttcagtacg
    11161 aaaattgctt tcattgtcga tacccttttt acgtgaactt gcgcactagt taactggttc
    11221 gatgattaat tgtcaacagc tcatttcaga atatttgcca gacccgttat gatgtcggcg
    11281 caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg cagtgattta
    11341 cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag cattggtgca
    11401 ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc gaccatgaca ccacagctgc
    11461 aatcgcgcca gcaagagagg aaatttcacg ttcgggactg gcgctgaatc ttattcccgg
    11521 cgtggaaatt tccacggtct gggaaaatca tgaaattcat attgtcgggc tgaatattga
    11581 tattactcat ccgctaatgt gtgagttcct cgcgcagcag acagaacggc gcaatcagcg
    11641 ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata cctggggcgc tggaaggcgc
    11701 acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat tttgcgcgtt tccttgtgga
    11761 gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag tatctggcgc gcgggaaaac
    11821 cggatacgtt ccgccacagt ggtgtacaat agaacaagct attgatgtca ttcatcattc
    11881 tggcggtaag gcggtgctag ctcatcctgg gcggtacaat cttagtgcta aatggctgaa
    11941 aagattggtt gcgcattttg ccgaacacca cggcgacgcg atggaagtcg cgcagtgcca
    12001 gcaatcgccc aatgaacgca cccagctggc cacacttgcg cgtcagcatc atttatgggc
    12061 atcacaagga tctgattttc atcagccatg cccgtggatc gaactgggtc gtaaactctg
    12121 gctgcccgca ggcgttgaag gcgtctggca gctatgggaa cagccgcaga acaccacaga
    12181 gagggaatta tgagccagtt tttttatatt catcctgata acccgcagca acgtctgatc
    12241 aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg tctatccaac tgattccggc
    12301 tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg agcgtatttg tcgtattcgc
    12361 cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg atctttctga actctcgacc
    12421 tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa acaacacgcc gggcaactat
    12481 accttcatcc tgaaggggac gaaggaagtg ccacgccgtc tgttgcagga gaaacgcaaa
    12541 accatcggta tgcgtgtgcc ttctaacccg attgcgcagg cgttgcttga agcactgggc
    12601 gaaccgatgc tttccacttc gctaatgctg ccaggcagcg aatttaccga atccgatccc
    12661 gaagaaatta aagaccgtct ggaaaaacag gtagatttga ttattcacgg cggctatctc
    12721 gggcagaaac caacaacggt tattgatctt accgatgata cgccggtcgt ggtgcgtgaa
    12781 ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact ctgtatacta cgcggcctta
    12841 aaatggcgct atacggcgcc attgattctc ctgttcgacg cctgtgaagg cgacaccaaa
    12901 aggaagctct atgagcgaaa aattacagaa agtgctggcc cgcgctggcc acggctcccg
    12961 ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt gttgatggca aaattgctaa
    13021 actcggcgat cgcgttgaag ttacccctgg gctgaaaatt cgtatcgatg gtcatctgat
    13081 ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg gcctattaca aaccggaagg
    13141 tgagttgtgt acccgtaacg atccggaagg gcgtccgacg gtgtttgacc gtctgccaaa
    13201 gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac gttaacacct gtggtctgct
    13261 gctgttcacc accgatggtg aactggcaaa ccgtttaatg catccaagcc gtgaagttga
    13321 acgtgaatac gccgtgcgtg tatttggtca ggttgacgac gcgaaactgc gtgatttgag
    13381 tcgtggcgtg caactggaag atggtccggc agcttttaaa accatcaagt tcagcggcgg
    13441 cgaagggatc aaccagtggt acaacgtgac tctgaccgaa ggccgtaacc gtgaagttcg
    13501 tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg atccgtgttc gttacggtga
    13561 tatcccactg ccaaaaggtc tgccacgcgg tggctggacg gagctggacc tcgcccagac
    13621 taactatctg cgcgaactgg tggagcttcc gccagaaacc agctctaaag tcgctgtaga
    13681 aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt gcggtgaaac gtcacagtca
    13741 ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt taatcaaagt atgccggacg
    13801 tcatatccgg catttttaca gattaataat caatccctat ttgcgcctta acaccggcat
    13861 caaacgcatg tctgatgggg cgcagctcac ttaccgtatc cgccagttca agaatatccc
    13921 gatgacagcc tcgacccgtg ataatcaccg tctgttgatg tggacgttca tttaacgcct
    13981 gcaccacttc ttccagtggc aaatagtcat acgccaccat atacgtcagt tcatcaagca
    14041 aaaccatatc cagtgaggaa tcagcaagca tccgctttgc atgttgccag acttcgcggc
    14101 aggcggcggt atcagactcg cggttttgtg tatcccaggt aaagcccgtt gccatcacct
    14161 gaaactcaac gccatgtggc tccagcagat tgcgttcgcc attaggccag gtgcctttaa
    14221 taaactgcac gacgcccact ttttttccgt gaccaactgc gcgtgttgcc gtaccaaatg
    14281 ccgcggtggt tttgcctttt ccattgccgg taaagacgat gataataccg cgttcatcct
    14341 gggcctgggc cacacgagca tctacttttt ctttcactcg ctgctgacgc tgttggtagc
    14401 gttcatcact cattgggaaa ttcctggttt acggcccggt tgggcgtcaa aggtcatgcc
    14461 ggttttacgg cggctgtcat cgcccatcag ccagaggtag agcggcatga tatcagcggg
    14521 tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt gcgcgcattg cggtgcgcgt
    14581 accgcctggg ttaatgcagt tgacgcgcag gcgctgctga tattcatcgg ccagtacctg
    14641 catcatccct tcggtggcaa atttcgacgc tgcatatgca ccccagttgg ctcgtccctg
    14701 acgtccaacg cttgatgaag taaagaccag tgaaccggcg tccgatttga gtaataaagg
    14761 aagcagtgcc tgggtgagca taaaggtggc attaacgttg acctgcatga cgtcctgcca
    14821 gacctgcgga ttttgttcgc tcattgggca aacatcgccg agcaatccgg cattatgcaa
    14881 aacaccatcc agacgcggat aattaacgac aatgcgctgt gccagttgtt ggcaattttc
    14941 ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc tgacgcccag tttcttcgtt
    15001 tatgtggctg gctacctgac gtaatttttc ttcattacgg cccaacagaa tcactgtcgc
    15061 accatagcgt gcatacgtca tcgcggcttc acgaccaatc ccatcgctgg ctcccgtcac
    15121 caggataatg cgatcattga gtaaatcttg ttttggctgg taatgcatgg ctactcctca
    15181 acgacgttgt ctgtcgcgct gccagtgtac tttatgactt tggggcttta tgcctgaaac
    15241 agaagggcat ttcaatcagc ctggggcgat aacaccgcaa aatggatact ttgtcatact
    15301 ttcgctgcaa taacatctct gcgagacggc ttaacatgcc tgttgtaaac tgtgagccaa
    15361 agcgttgttt aaccaaggtg gggactcgtg gaattgttgt ctgaatatgg tttgtttttg
    15421 gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg ccgccattat tgtcaatgtt
    15481 gctcaacgta ataaacgcca gcgtggcgag ttacgggtca acaatctcag cgaacagtat
    15541 aaggagatga aagaagaact ggccgcggcg ctgatggact cacatcagca aaaacagtgg
    15601 cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag cagcaaaagc gaaagccaaa
    15661 ctgggcgagg tggcaactga cagtaaacct cgcgtctggg tgctggattt taaaggcagc
    15721 atggacgccc atgaagtgaa ctcgctacgt gaagagataa cggcggtact cgcagcattc
    15781 aaaccgcagg atcaggttgt gctccgtctg gaaagccctg gtggcatggt gcatggttac
    15841 gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa acattccttt aactgttacg
    15901 gtagacaaag tcgctgccag cggcggttac atgatggcct gtgtggcgga caaaattgtt
    15961 tccgcaccgt ttgctattgt gggttccatt ggggtggtgg cgcaaatgcc caactttaac
    16021 cgcttcctga aaagcaaaga tattgatatc gaactgcaca ccgccgggca gtataagcgc
    16081 acgctgacct tgctgggtga aaataccgaa gaagggcggg agaaattccg cgaagagctg
    16141 aacgaaacgc atcagttatt taaagatttt gtgaagcgta tgcgtccgtc tctggatatt
    16201 gaacaggtgg caacgggtga acactggtac ggacaacagg cggtagagaa aggcctggtt
    16261 gatgaaatca acaccagtga tgaagttatt cttagcctga tggaaggccg tgaagtggtt
    16321 aatgtacgct atatgcagcg taaacgactc attgaccgat tcaccggcag cgcggcagag
    16381 agcgccgatc gattgttgct acgctggtgg cagcggggtc aaaagccatt gatgtaaaag
    16441 acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa ccagatgata tttttctgaa
    16501 agcacatggg ccaggtgttt gaacatatta aacaccgcgg tgcttttggc tgttggcaat
    16561 ccttgttcat ctaaaaagta gtcgccggta aataccagca cgccattacg ctgcgtgacg
    16621 ccggtggctt caatccctgc gagcgtatcc tcatgctcac gaatgatttt gttggcctct
    16681 ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt tcattattta ctccttaaac
    16741 aaggacatta gtctacgcca ggcatggctt gcagacaaat ataccacgct ggtggcaaga
    16801 gcgccttact ggcaactttg gattttgcat gctaataaag ttgcgtatcg gattttatca
    16861 ggtacagtgt gacgctttcg tcaatctggc aatagatttg cttgacattc gaccaaaatt
    16921 ccgtcgtgct atagcgcctg taggccaaga cctgttaact cagtcacctg aattttcgtg
    16981 aacagagtca cgacaagggg ttgatatccg cagagagcga gtccatatcg gtaactcgtt
    17041 gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa gaatcaaatt aggtaaggtg
    17101 aatatgggta aagctcttgt catcgttgag tccccggcaa aagccaaaac gatcaacaag
    17161 tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc acatccgcga tttgccgacc
    17221 agtggctcag ctgccaaaaa gagtgccgac tctacctcta ccaagacggc taaaaagcct
    17281 aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg ttgacccgtg gcacaattgg
    17341 gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg tctctgaact gaaacaactg
    17401 gctgaaaaag ccgaccacat ctatctcgca accgaccttg accgcgaagg ggaagccatt
    17461 gcatggcacc tgcgggaagt gattggtggt gatgatgcgc gctatagccg agtggtgttt
    17521 aacgaaatta ctaaaaacgc gatccgccag gcatttaaca aaccgggtga gctgaatatt
    17581 gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc gcgtggtggg gtatatggtt
    17641 tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg ccggtcgtgt gcagtcggtg
    17701 gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag cgttcgtacc ggaagagttc
    17761 tgggaagtcg atgccagcac gactacgcca tctggtgaag cgttggcgtt gcaggtgact
    17821 catcagaatg acaaaccgtt ccgtccggtc aacaaagaac aaactcaggc tgcggtaagt
    17881 ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag acaaaccgac aaccagtaaa
    17941 cctggcgctc cttttattac ctctacgctg caacaagctg ccagcacccg tcttggtttt
    18001 ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg aagcaggcta tatcacttac
    18061 atgcgtaccg actccactaa cctgagtcag gacgcggtaa atatggttcg cggttatatc
    18121 agcgataatt ttggtaagaa atatctgccg gaaagtccga atcagtacgc cagcaaagaa
    18181 aactcacagg aagcgcacga agcgattcgt ccttctgacg tcaatgtgat ggcggaatcg
    18241 ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt taatctggcg tcagttcgtt
    18301 gcctgccaga tgaccccagc gaaatatgac tccacgacgc tgaccgttgg tgcgggcgat
    18361 ttccgcctga aagcacgcgg tcgtattttg cgctttgatg gctggacaaa agtgatgcct
    18421 gcactgcgta aaggcgatga agatcgtatc ttaccggcag tcgataaagg cgatgctctg
    18481 acgctcgttg aactaacacc agcccagcac tttaccaagc cgccagcccg tttcagtgaa
    18541 gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc gtccgtctac ctatgcgtcg
    18601 atcatttcga ccattcagga tcgtggctat gtgcgagtag aaaatcgtcg tttctatgcg
    18661 gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa atttccgcga gttaatgaac
    18721 tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg tggcaaatca cgaagcagag
    18781 tggaaagctg tactggatca cttcttctcg gatttcaccc agcagttaga taaagctgaa
    18841 aaagatccgg aagagggtgg tatgcgcccg aaccagatgg ttctgaccag cattgactgc
    18901 ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta ccggggtatt ccttggctgt
    18961 tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca ccattaacct ggtgccggaa
    19021 aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa ccaacgcgct gcgcgcaaaa
    19081 cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc tcatcgatcc gaaacgtaag
    19141 ttgcatgtct gtggtaataa cccaacctgc gacggttacg agatcgaaga gggcgaattc
    19201 cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa aatgtggctc tgaaatgcac
    19261 ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca acgaagagtg taaaaacaca
    19321 cgtaagattt tacgtaacgg cgaagtggca ccaccgaaag aagatccggt gccattacct
    19381 gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc gtgacggtgc tgccggtgtg
    19441 ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc gtgcgccact ggtggaagag
    19501 ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt atctggccga tgcgccacag
    19561 caggatccgg aaggtaataa gaccatggtt cgctttagcc gtaaaaccaa acagcaatat
    19621 gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag cattttatgt tgatggcaaa
    19681 tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt tttataaaca aagggtcgcg
    19741 aaagcggccc ttttttattg catattattt tttcttcaca cctatacact aaggctataa
    19801 atgatatagt ggttatagtt agcacctttt ttattattaa atcgtattag tcacccgcca
    19861 ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg atggtttaac atgaaattac
    19921 aacaacttcg ctatattgtt gaggtggtca atcataacct gaatgtctca tcaacagcgg
    19981 aaggacttta cacatcacaa cccgggatca gtaaacaagt cagaatgctg gaagacgagc
    20041 taggcattca aattttttcc cgaagcggca agcacctgac gcaggtaacg ccagcaggac
    20101 aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt cgatgccata aaatcggttg
    20161 ccggtgagca cacctggccg gataaaggct cgctgtatat cgccaccacg catacccagg
    20221 cacgctacgc gttaccaaac gttatcaaag gctttattga gcgttatccc cgcgtttctt
    20281 tgcatatgca ccagggcccg ccgacacaaa ttgctgatgc cgtctctaaa ggcaatgccg
    20341 atttcgctat cgccacagaa gcgctgcatc tgtatgaaga tttagtgatg ttaccgtgct
    20401 accactggaa tcgggctatt gtagtcactc cggatcaccc gctggcaggc aaaaaagcca
    20461 ttaccattga agaactggcg caatatccgt tggtgacata taccttcggc tttaccggac
    20521 gttcagaact ggatactgcc tttaaccgcg cagggttaac gccgcgtatc gttttcacgg
    20581 caacggatgc tgacgtcatt aaaacttacg tccggttagg gctgggggta ggggtcattg
    20641 ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt gcgcgttgat gctcacgata
    20701 tcttcagcca cagtacaacc aaaattggtt ttcgccgtag tacttttttg cgcagttata
    20761 tgtatgattt cattcagcgt tttgcaccgc atttaacgcg tgatgtcgtt gatgcggctg
    20821 tcgcattgcg ctccaatgaa gaaattgagg tcatgtttaa agatataaaa ctgccggaaa
    20881 aataatttct tgcgttattt tcggcacctt ttatgtagcg aaggtgccgg aatatattct
    20941 cttttgttac ttatttctat accgtgcaca atatttttat ccctcatatt tatagggtaa
    21001 attacctgta aagtagtgtc taattacccg cctcgcaaat tccagttttc aaatatctat
    21061 ttcaggtcaa aagattgaat tattcactgg agacgattcg taaattcgct ggatttttga
    21121 ctaaagtttc tttaggattt gtctcatcgt atgattaatt acaccaatca ttcaacgtct
    21181 tgctgataag tgatggcgat tatatgaggt tagcaatgcc ttctggaaat caggaacccc
    21241 gccgtgatcc tgaattaaaa cgtaaagcct ggctggcggt ttttcttggt tctgcacttt
    21301 tctgggtggt tgtcgcactg ctgatttgga aagtgtgggg ataactatgg tcggtcagga
    21361 gcaactggag tcgtcaccat tatgccagca tagtgacaat gaaccagaaa cgaaacggga
    21421 atgttccgtc gttattccag acgactggca actaacatcg cagcagcaag cctttataga
    21481 actgtttgct gaagatgatc agccgaaaca ataattatca tcattcttat tacccatttt
    21541 taatgaatta aagggctttt aatacaccgc agcaataaca gcttgagtta tctcaacaca
    21601 aaataataac cgttaagggt gtagcctatg atcaacacaa atatgaaata ttggtcctgg
    21661 atgggcgcgt tttctctgtc gatgctcttc tgggccgaac tcctctggat cattactcac
    21721 tgatccttga ccccgctgcg gcggggttgt catttgcttt gccacaaggt ttctcctctt
    21781 ttatcaattt gggttgttat caaatcgtta cgcgatgttt gtgttatctt taaattcacc
    21841 ctgaagagaa tcagggcttc gcaaccctgt cattaaggag gagctatgtc gtcaacccta
    21901 cgagaagcca gtaaggacac gttgcaggcc aaagataaaa cttaccacta ctacagcctg
    21961 ccgcttgctg ctaaatcact gggcgatatc acccgtctac ccaagtcact caaagttttg
    22021 ctcgaaaacc tgctgcgctg gcaggatggt aactcagtta ccgaagagga tatccacgca
    22081 ctggcgggat ggctgaaaaa tgcccatgct gaccgtgaaa ttgcctaccg cccggcaagg
    22141 gtgctgatgc aggactttac cggcgtacct gccgttgttg atctggcggc aatgcgcgaa
    22201 gcggttaaac gcctcggtgg cgatactgca aaggttaacc cgctctcacc ggtcgacctg
    22261 gtcattgacc actcggtgac cgtcgatcgt tttggtgatg atgaggcatt tgaagaaaac
    22321 gtacgcctgg aaatggagcg caaccacgaa cgttatgtgt tcctgaaatg gggaaagcaa
    22381 gcgttcagtc ggtttagcgt cgtgccgcca ggcacaggca tttgccatca ggttaacctc
    22441 gaatatctcg gcaaagcagt gtggagcgaa ttgcaggacg gtgaatggat tgcttatccg
    22501 gatacaatcg ttggtactga ctcgcacacc actatgatca acggccttgg cgtgctgggg
    22561 tggggcgtgg gtgggatcga agcagaagcc gccatgttag gccagccggt ttccatgctt
    22621 atcccggacg tagtgggctt taaacttacc ggaaaattac gtgaaggtat taccgccaca
    22681 gacctggttc tcactgttac ccaaatgctg cgcaaacatg gcgtggtggg gaaattcgtc
    22741 gaattttatg gtgatggtct ggattcacta ccgttggcgg atcgcgccac cattgccaat
    22801 atgtcgccag aatatggtgc cacctgtggc ttcttcccaa tcgatgcagt aaccctcgat
    22861 tacatgcgtt taagcgggcg cagcgaagat caggtcgagt tggtcgaaaa atatgccaaa
    22921 gcgcagggca tgtggcgtaa cccgggtgat gaaccgattt ttaccagtat gttagaactg
    22981 gatatgaatg acgttgaagc gagcctggca gggccaaaac gcccacagga tcgcgttgca
    23041 ctgcccgatg taccaaaagc atttgccgcc agtaacgaac tggaagtgaa tgccacgcat
    23101 aaagatcgcc tgccagtcga ttatgttatg aacggacatc agtatcagtt acctgatggc
    23161 gctgtggtca ttgctgcgat aacctcgtgc accaacacct ctaacccaag tgtgctgatg
    23221 gccgcaggct tgctggcgaa aaaagccgta actcttggcc tcaagcggca accgtgggtc
    23281 aaagcgtcgt tggcaccggg ttcgaaagtc gtttctgatt atctggcaaa agcgaaactg
    23341 acaccgtatc tcgacgaact ggggtttaac cttgtgggat acggttgtac cacctgtatt
    23401 ggtaactctg ggccgctgcc cgatcctatc gaaacggcaa tcaaaaaagg cgatttaacc
    23461 gtcggtgcgg tgctgtccgg caaccgtaac tttgaaggcc gtatccatcc gctggttaaa
    23521 actaactggc tggcctcgcc accgctggtg gttgcctatg cgctggcggg aaatatgaat
    23581 atcaacctgg cttctgagcc tatcggccat gatcgcaaag gcgagccggt ttatctgaaa
    23641 gatatctggc catcggcaca agaaattgcc cgtgcggtag aacaagtctc cacagaaatg
    23701 ttccgcaaag agtacgcaga agtttttgaa ggcacagcag agtggaagga aattaacgtc
    23761 acacgatccg atacctacgg ttggcaggag gactcaacct atattcgctt atcgcctttc
    23821 tttgatgaaa tgcaggcaac accagcacca gtggaagata ttcacggtgc gcggatcctc
    23881 gcaatgctgg gggattcagt caccactgac catatctctc cggcgggcag tattaagccc
    23941 gacagcccag cgggtcgata tctacaaggt cggggggttg agcgaaaaga ctttaactcc
    24001 tacggttcgc ggcgtggtaa ccatgaagtg atgatgcgcg gcaccttcgc caatattcgc
    24061 atccgtaatg aaatggtgcc tggcgttgaa ggggggatga cgcggcattt acctgacagc
    24121 gacgtagtct ctatttatga tgctgcgatg cgctataagc aggagcaaac gccgctggcg
    24181 gtgattgccg ggaaagagta tggatcaggc tcaagccgtg actgggcggc aaaaggacct
    24241 cgtctgcttg gaattcgtgt ggtgattgcc gaatcgtttg aacgaattca ccgttcgaat
    24301 ttaattggca tgggcatcct gccgctggaa tttccgcaag gcgtgacgcg taaaacgtta
    24361 gggctaaccg gggaagagaa gattgatatt ggcgatctgc aaaatctaca acctggcgcg
    24421 acggttccgg tgacgcttac gcgcgcggat ggtagccagg aagtcgtacc ctgccgttgt
    24481 cgtatcgaca ccgcgacgga gttgacctac taccagaacg acggcatttt gcattatgtc
    24541 attcgtaata tgttgaagta acaagtattt gcttgccggt tattttgctt ccggcaagca
    24601 aatgaattac acgatgcaag agggttattt gttcagcaaa tggcccattt tctcggcttt
    24661 ggtatcgaga taatgttcgt tattggggtt acgacctaca atcaatggta cgcgttcaac
    24721 aatattaatc cctgcttcgg tcagaatttc gacttttttc gggttattgg ttaacaagcg
    24781 gacttcattg acgccaagga gtttgaacat atcagcgcaa agagtgaagt cgcgctcatc
    24841 agcggcgaag cctaactggt ggttagcctc tacggtatcg taaccttgat cctgcagtgc
    24901 gtaagcgcgg attttattca gcagaccaat gttacgacct tcctgacggt gatacagcaa
    24961 aataccacgg ccttcctcgg caatttgcgt caatgccgct tcgagctgga agccacaatc
    25021 gcagcgcaag ctgaacaggg cgtcaccggt cagacattcg gaatggacgc gcgcaagtac
    25081 cggggtatgc ccggaaatat cgccatagac tagcgcgaca tgatcgtgtc cggttgccag
    25141 ttcttcaaat cccaccatca ggaaatcgcc ccatggggtt ggcagtttgg cttctgccac
    25201 acgtttaagc tgcatgaaat tctccagata atgctggttc tgtattggct tattttgcca
    25261 taacgagaag gggttcacct aatcacgacg cgtcgatcgt tcacggaatg gcacaaatct
    25321 gtcaattttt cttggaactg gcattttcag ttatgattgt gggacttatc aaaaaggaga
    25381 ggccatgcgt tcgattgccc gacgtaccgc agtgggagct gcactattgc ttgtcatgcc
    25441 agtagccgta tggatttctg gctggcgttg gcaacctgga gaacaaagtt ggctactaaa
    25501 agcggctttt tgggttactg aaactgtcac ccagccctgg ggcgtcatta cacatttgat
    25561 tttgttcggc tggtttctct ggtgtctgcg ttttcgcatt aaggctgcct ttgtattatt
    25621 tgccattctg gcggcggcaa tccttgttgg acaaggcgtt aaatcctgga tcaaagacaa
    25681 agtacaggaa ccacgacctt ttgttatctg gttggaaaaa acacatcata ttccggttga
    25741 tgagttctac actttaaagc gagcagaacg cggaaatcta gtaaaagaac agttggctga
    25801 agagaaaaat attccacaat atttgcgttc acactggcag aaagagacgg ggtttgcctt
    25861 tccttccggt cacacgatgt ttgctgccag ttgggcactg ctggccgttg gtttgctgtg
    25921 gccgcgtcgg cgaacgttaa ccattgctat cttgttggtc tgggcaacgg gagtcatggg
    25981 aagccgcctg ctgctcggga tgcattggcc acgcgatctg gtggtagcta cgttgatttc
    26041 gtgggcgctg gtggcggtgg caacgtggct tgcgcaacga atttgtgggc cattaacacc
    26101 acctgcggaa gaaaatcgcg aaattgcaca acgagaacaa gaaagttaac gctggttgat
    26161 tttccgaatt taggccttaa atcatcaaca atgcgcgtgg atgccatttc gcagacggcg
    26221 cgaaaatggt actttaaagg gctattgcgg taagttgacc ataatttatt cgctctaacc
    26281 acataacggg aagtaatgtg aaatatttac tcattttctt actggtgtta gcgatcttcg
    26341 tgatttcggt cacgttgggt gcgcagaacg atcaacaggt gacgtttaat tatctgttag
    26401 cgcaagggga gtaccgtatt tccacattgc tggcggtatt gtttgctgcg gggtttgcta
    26461 tcggttggtt gatttgtggc ctgttctggc tgcgagttcg tgtttccctg gcgcgcgctg
    26521 aacgtaaaat aaagcgactg gaaaaccagc tttcacccgc gactgacgtg gctgtagtgc
    26581 cgcactcgtc agcggcgaag gaataacttt ctatgctgga gttgttgttt ctgcttttgc
    26641 ctgtagccgc tgcctatggc tggtatatgg gccgcagaag tgcgcaacaa aacaagcaag
    26701 atgaagccaa ccgcttgtcg cgtgattacg tagcgggggt taacttcctg cttagtaatc
    26761 aacaggataa agcggtagac ctgtttctcg atatgcttaa agaggataca ggcaccgttg
    26821 aagcccacct tacgctcgga aacctgttcc gttcgcgtgg cgaagttgat cgcgctattc
    26881 gcatccatca gaccctaatg gaaagcgcct cgctgaccta tgaacagcgt ctgttggcga
    26941 ttcaacaact ggggcgtgat tacatggccg ccgggttata cgaccgcgcg gaagacatgt
    27001 tcaatcagct gaccgatgaa actgacttcc gcattggcgc gctgcaacag ttgctacaaa
    27061 tctaccaggc taccagtgag tggcagaaag caattgatgt tgccgaacgc ctggtgaagc
    27121 tgggtaaaga taaacagcgc gtcgaaattg cccatttcta ctgtgagtta gccctgcagc
    27181 atatggccag cgacgatctc gatcgtgcca tgaccttgct aaaaaaaggg gcggcggcag
    27241 ataaaaacag cgcccgcgta tccatcatga tgggacgcgt gtttatggcg aaaggagaat
    27301 acgccaaagc cgtcgaaagt ttgcaacgcg tcatatccca ggacagagaa ctggtcagcg
    27361 aaacgctgga aatgttgcaa acctgctatc agcagttggg taaaactgcc gaatgggcag
    27421 aattcctgca acgcgcggtg gaagagaaca ccggtgccga tgctgaattg atgcttgctg
    27481 atatcatcga agcgcgcgac ggtagtgagg ctgcacaggt ctatattaca cgccagcttc
    27541 agcgtcatcc gaccatgcgt gtattccata agctaatgga ttaccactta aatgaagcgg
    27601 aagaagggcg cgccaaagag agtctgatgg tgctgcgtga catggttggc gagaaggtgc
    27661 gtagtaagcc tcgttatagc tgccagaaat gtggttttac cgcatacacc ctctactggc
    27721 attgtccgtc ttgtcgtgcc tggtcaacta ttaaaccgat tcgcggtctt gatggcctgt
    27781 aatttttaaa aaaatccgac tttagttaca acatactagt tattgatgtc ccattgtgct
    27841 ccggcaacga cggagcagaa aaagcctgct aggggaaaaa tcgcaactgt taatttttta
    27901 ttttcacggg tagaatgctc gccgtttacc tgtttcgcgc cactcccggt gcccatcatc
    27961 aagaaggtct ggtcatgacg ttaactgctt catcttcttc ccgcgccgtt acgaattctc
    28021 ctgtggttgt tgcccttgat tatcataatt gtgatgacgc gctgtccttt gtcgacaaga
    28081 tcgacccacg cgattgtcgt ctgaaggtcg gtaaagagat gtttacattg tttgggccac
    28141 agtttgtgcg cgaacttcaa cagcgtggtt ttgatatctt tcttgacctg aaattccacg
    28201 atatccccaa cactgcagcg cacgctgtcg ctgctgcagc tgacttaggc gtgtggatgg
    28261 tgaatgttca tgcctccggt ggggcgcgta tgatgaccgc agcgcgtgag gcactggttc
    28321 cgtttggcaa agatgcaccg cttttgattg ctgtgacagt gttgaccagc atggaagcca
    28381 gcgacctggc cgatcttggc gtgacactgt cacctgcaga ttatgcagaa cgtctggcag
    28441 cactgacgca aaaatgtgac cttgatggtg tggtgtgttc tgctcaggaa gctgtgcgct
    28501 ttaaacaggt attcggtcag gagttcaaac tggttacacc gggcattcgt ccgcagggga
    28561 gtgaagctgg tgaccagcgc cgcattatga cgccagaaca ggcgttggcg gctggtgttg
    28621 attatatggt gattggtcgc ccggtaacgc aatcggtaga tccagcgcag acactgaaag
    28681 cgatcaacgc ctctttacag cggagtgcat gatgagtgat tccaacagcc gtctggtcta
    28741 ctcaacggaa accgggcgta ttgatgagcc caaagcggcc ccagtacgcc ctaaaggcga
    28801 tggagcggtg cgtattcagc gtcagaccag tggacgtaaa ggtaagggcg tttgcctgat
    28861 taccggtatc gatctcgatg atgccgaact gacaaaactt gcagcggaac tgaagaaaaa
    28921 atgcggctgc ggcggagcag ttaaagatgg agttattgaa atccagggcg ataagcgtga
    28981 tttattaaaa tcactacttg aagcgaaagg gatgaaggta aaactcgcag gcggttaaca
    29041 taaaaagcca cggatatatc cgtggctttc gaatatttta ctgtgtgtat tattcagtta
    29101 tttcgtactg aacagaatca agcgtattat aattgcgagt cgataatcga catttattta
    29161 cgattattta ccgacctggt gaccaataac accaccgaca gctgcaccac ctaatgtacc
    29221 caacgtactg ccatcggtca gtactgcacc gcctaatgcc cctgcacccg cgccgattgc
    29281 ggtgttgcgg tcccgtttag accagttaga acaggcactc agagacattg ccaaagtaat
    29341 tgccagaaca gccgcggtca tttttttgct cgttacaaac ataatactct ctcctgaatt
    29401 tatgattcac ggaagtaagc tctctataac tataatagct aagaataagc ctggtgaaat
    29461 tattccgtga aatctgctcg cgcgcagggt tatatcacgc aggtgataat tacttcctgt
    29521 tatatcgctg ataataattt tatatcttga gagtgttaat aacaggtaaa tagtcttaat
    29581 tatcaaccag gaatcatctt agagcggatg atttgccaaa ctgcaaatca tccgtagaga
    29641 agggaaatgg ttaaatgtca acgacgtgaa cggtgagttt actgtgctca agatgcatca
    29701 gatcgctggc gcgtattttc gaatcggtaa tcacgcgatt gaatcgctca acgggaccga
    29761 tggagtatga atgtacagca ccaaatttcg agctgtcagt caggactatc gcttcgcact
    29821 ctttttccag cacggcattg accacatcgg tgcgcatcat atcgcgaccg gtgaatccag
    29881 tttcaggttg ccagccatca ataccaataa atgctttgct gaaatgtacc tgctggatgc
    29941 actggcgtgt caaagggcca accatacttt cgcttttttt ctggtacacg ccaccgagca
    30001 aaatgacttc acaaggcgcg tctttcagca aatgcgcgat gtagctgctg accgtgatga
    30061 tagtgacatt tttcttctgc tcccccagag tccgggcgag cagggcattg ctgctgccat
    30121 tttcgataaa gatggtttcg cccggttgaa ccagtgacgc ggcaaactcg gcgagttcac
    30181 gcttcagcgt atagttgctc atcatacggg tttcgacgtc atcactatca agcgaaactg
    30241 caaagccatg tgcacggcgg aggtaactca gtttttcgag ggtgttgaga tcctggcgaa
    30301 tggtaacttc agaaactcca gtggcttttg ccagatcggt tacgctaacc tgaccctggt
    30361 caatgaccat ctgtagaata gtttgttgtc gggaattcat cgctgtaatt cttataacgt
    30421 tataatacta aaaaaatact gacgcgccgg gcatcctcaa ataggaaaac ctgaaggcac
    30481 gattgttaaa tctcccacgg tgtcttcggg atgtctggcg tagattcagc aacctggcgc
    30541 aagagttctg ctttaagtat ctcgagatta tggatcgcag agtggtaatc gcctgccaca
    30601 aggatatcta acacaatatc aatacgttcg gcaacgcgct gggcatcaaa ttcagacatc
    30661 aaggacatcc ttatagcaaa agggggaaaa tgaataatgc aaaaattgcc gctaaaagag
    30721 aagtgtttaa cagcaacggc taattatcat ccaggaatac gatatataat gacgggatat
    30781 agcgctaagt atatatattc atctacttat gcgcgcttca gatagcgttt ataccagcgt
    30841 tcgaaggcga cggcgggcat cggtttggca aacaaaaatc cttgccgctc attgatcccg
    30901 ttcttggtta aaaaagcatc ttcctttgca ctctctacac cttcggcgat cacctgaaga
    30961 ttcaatgcct gggccacagc gacgatcgcc cggaccagtg actgcgagac aggttgtttg
    31021 tgaatatctc gaacaaaaac ctggtcaagt ttgatggcat cgatcggaaa gcgcgccagt
    31081 tgcgaaagtg aagagtagcc ggtaccaaaa tcatccagat gcacttgcgc acctagttgg
    31141 ctaaattgtt gaataacaga cagtgccagt tcatcattct caatcagaca actctctgtc
    31201 agttcaacat ctatagggca gtattcaaaa ttgagttcct ggagaacctg tttcagggcg
    31261 gtgaaaatgg tttgatcggc gagctgacgt gcagaaatat ttaccgccac tcgcaggttt
    31321 atgcctttat cccgccactt tgccacctgg cgtacgacat cgagaatcac ccaacggcct
    31381 aaaggcacaa ttagccctga ctcttcggcg taggaaatga agtccaacgg tggaatcaac
    31441 ccacgttcag gtgactgcca acgtactagt gcttccagac tgcgcacttc gccacgctag
    31501 gtgattttcg ataaccaact gatcgttttc cagtgcttta cgcaagttgg tatccagcca
    31561 gagatattca aatacccgct gattcatttc tggagtaaaa acgcaaaatt gtcctcgtcc
    31621 gccttccttc gctgtgtaca ttgctgtgtc ggcgtgacga ataatagtcg cgctgtccga
    31681 accatgttcg ggagagagcg aaatacctac tgaacagctg gtataaactt caattaaacc
    31741 aatacgaaag gggaggcgta agcgggtcaa aattcgtgat gccattgctt ccagcgcgct
    31801 ttgtgaggta ttggatgcca gtaccagaaa ctcatcgcca cctggacgcg ccaacacctg
    31861 gtcatgttcg agacagctta aaatagccaa tgacacgtcg cgtaataact ggtcaccaaa
    31921 caaatgccca taggcgtcgt tgaccttttt aaaattatcc aaatcaagat acacaacccc
    31981 aactttattg ttatctgcct gattaatagc gtgatcgatt aactcctgca ttgcgttacg
    32041 attcggcagt ccggtgatac tgtcggtatt tgccagaata cgcagtcgct cctgagtgcg
    32101 gcgctcttcg gtaatgtcgg taccggaaca gattaaaaaa atctcgtttt tgccactgcc
    32161 gctgtggaca aatttattgc gaaacagaaa cagccgctgg cctttacgtg ttggtatcca
    32221 cagttcgact tcatatgcat tgccgcttcg aaaaaataca cggttattgc gcctggatgc
    32281 cgcagcttca cgacggctca taaacagttt aaacacgctt tacccaatga cgtcgtgttc
    32341 tttcaaccct gtgtaatctt cacataaccg attgaagcgt tggatattcc cccggctgtc
    32401 gagaatcact attgcggaat gtgcttcaga aactacctgc tcagcaaagg aaagcccgtg
    32461 tgataaatca cgagcaaccg ccggtgtgtc atgccatgca gacgcgttgc cagcccattc
    32521 ccgtttattg atttttcgtc ctaccagatg cacagaaaga tcgctttcat cgacggtcag
    32581 actcatcatc aggctggagg tgatgaccgt catttccctg atgcgagcag cctgttcggc
    32641 agataactgc aatgtacgat caggttctgt ggttttggtg gtagaaaagc gcaaagcatc
    32701 gctgctttcc gtcaaccgcc agtatggatt gtgcgatccg agaaagttgt acaacgttgt
    32761 ggactcccta acggttttca tgatgcatat cctccggtta acagcggagt ccgcggttaa
    32821 ataaaaggaa caacatgact tacatgaaat taacggcggc taaacgcatt gttttttata
    32881 tatttatttg taatccagtt ttggaaaaac gccagttttc aaacgaaagt cagttaaaaa
    32941 atctgcctgg atataacgaa ggtagagcgg ggaaataaac ggcccatcca tgaggaatgg
    33001 gccgtgaaag gagattacgc gaccgggcgc gcaataatgc tgcgggtttc catgcggact
    33061 tcggcaatgg tgacgtcaat aacgtcagtt actttgtaaa ccgtttcacc tttaatttgt
    33121 acagtgccgt tttcctggct gcaaaccagt tcatcgcgca cagcgtgtaa gaaaggtgcc
    33181 ggaataaagg cgatagcgcc gttatcaacc aaacgcacac gcatgccgcc acggctgata
    33241 tcgacaattt ccgccgcgaa acgggtgccg gtcccggctt tatctttcag gaagcgtgcg
    33301 tataaccagt cgccaacatc acgttctgcc atccggttga gacggcgacg ttcggccatt
    33361 tggacagtga tttcatcctg cggacgcgtc gcagtttctc ctttgataac cgctttcagc
    33421 agacggtggt tgatcatgtc gccatattta cggatcggcg aagtccaggt ggcgtatgct
    33481 tccagaccga ggccaaagtg aggaccgggt tcagagctaa tttcagcaaa tgactggaag
    33541 cgacgaatgc ggctgtcgag gaaaccagtt ggttgcgcat ccagttcacg acgcagtttg
    33601 cagaaaccgt ccagcgtgag cacttcttcg gcatcgacat gcagaccgtg cgttttcagc
    33661 aacgctgcca gcgcgtcggc attcgccgga tcaaagccca tatgcacgtt atagatgcca
    33721 aaaccgagct tatcgcgcag tacgcgggcc gcacaaatgt tagcggcaat catcgcttct
    33781 tcgacgatac ggttggcaat gcgacgaggc tcggcgacga tatccagcac ttcacctttt
    33841 tcaccgagaa taaagcggta atccgggcga tctttaaaca ccagtgcgtg gttatgacgc
    33901 cactcgccgc ggcgttggca aatttgcgct agcaaacgga cttgttcggc aattgcttca
    33961 ctttcaggct tccagtcacc ggtattttcc agccagtcag aaacctggtc atacaccagc
    34021 ttcgctttgg attcgatggt ggcggcaaag aattcgatat tatcttcagt ggtgccatca
    34081 gcggagagcg tcatgcggca tgccagtacc gggcggactt cattggcgcg cagtgagcag
    34141 agatcgtcag aaagctcgcg aggcagcata gggatgttga agccaggcag atagttggtg
    34201 aatgcgcgaa ttttcgcggc tttgtccagc ttgctgcctt cagcaatcca cgcggttgga
    34261 tcggcaatcg ccacaatcag ctgaagtttg tcatccggca gcgccttagc gaaaagggcg
    34321 tcatccatat cttcagtgct ggcactgtcg atggtgacaa aatccagcga ggtcagatct
    34381 tcacgaacca gaccttcatc gagcatttcg gtagcgacgc cgtctggtgc ttctttttcc
    34441 agattatggc gcgcaagggt aacccaccac ggtacaaagt ggtcgtcacc aaaagtgatg
    34501 tattgtgtca gttcagcata gaaagaacga tcgcctttca gcggatgacg gcgcatttcg
    34561 gcaaccgccc agtcgccttc tttaaactcg tggttcaggc cacgggctgc gcggcaagga
    34621 atggcgtctt ttaaaagtgg atgatcagga acgatggtca gacggtcatt tttgccctga
    34681 actttaccca cgaaacgagt caggaacggt tcaaccagtt cttctggctc tgcggattca
    34741 cgttcttttt cactgtggat caccgcgata attcggtcgc catgcatgac ttttttcatc
    34801 tgcggcggcg gaatgaaata acttttttgt gcgtcgactt ccaggaagcc aaagcctttt
    34861 tctgtggctt ttaccacccc ttcagcgcgt ggcgtctggg aatgcagttg ctgtttaagc
    34921 tgcgctagca gcgggttgtc ctgaaacata attgtctatt ttggtggcca ttagagcggc
    34981 tgacagtttt acgcgaatct gtctgacgcg gcaaggttaa tatgtctcac ccaacgcgat
    35041 ttttaagcga tttatccagc cacacagccc gctccatacc agcagattaa taatctgcgt
    35101 tgatgatttt tcgtgttcga gtaaagggct gaactgggcg gcgctaaagc gatcgggtgc
    35161 tcgggtcagc cattgtatcg cctgaacggt agcgcgctca acagaatgac ggtcagccca
    35221 tcggttaatt tcattttcgc ccttgcgcag aagggtgctg atttttttat attcatttga
    35281 tgaatccata caggtggaaa aacaagcagg actgccattt atacgcgcgc ataacaacgc
    35341 cgcaagctga ttatccggct gtgcattgac ccgtgtgctg tttaacaact tttcgagaag
    35401 ggaaagtgaa atagggtgga gagagagtgt ttcggccagt tggcaaagtt ctgcagttga
    35461 actttttgtg tgctcttcag gtagtttttc cacctcatag ctgcttcgcc agcgtattga
    35521 ttcatcagca aacagtgacg cgtcggcgta gttttgtatc gccagcccgg gtaaccagcg
    35581 taccggatgc ccgagataag cctgaaatgt cgcaattgtc cgcgcctgaa agccaataaa
    35641 accaatgatt tggttaatga tcacacagtc ccagacagta agaccgacgt cattcagatg
    35701 ctggcgcgat cggttgtcga taatagaagg cgagctagcc agttggcggg catattgcgt
    35761 gatttgtgcc aaccgatgat tactttccct ggaggaatca ggtccgggga gcggcgtaag
    35821 tcgcgccgaa tagtagttac ataaccgctg gacgccgcaa acctgagcaa ccgtcagggc
    35881 cgtcgacaag cgttcataag cggtgaaggt ttgtgaacgg gtcacggtaa gctggtcagg
    35941 gaacaactcc actgcaagaa cgcgcgcagg gaccagccat gaaaggaagg gggccagcgt
    36001 ctcttcatca aggatcacgt cgagcagaaa gggatcgctg acctttgcgg cttccgggac
    36061 cagaggcaga acgtcatact ccgtagtact ggattgcgtt tcatgatacc agtggctttt
    36121 gccggtgatg tggcgttgtt ccatgggcgc tccttggtcg taaaggaaat cgttatcctg
    36181 acgcaaggcg ggaaggggag aaagacggat cggcgataac aaatatcaga aaggtataac
    36241 agagataacg ggcggcagaa cgccgcccat ctttaccaac agaacgatta tttcagttcg
    36301 agttcgttca ttgcagcgat gctgaaaccg ccgtcaacgt ggactacttc accggagata
    36361 ccggcagaga gatcggagca caggaatgcc gcagagttac ccacatcttc aatagtaact
    36421 gtacggcgaa tcggggtaac ggcttcgcaa tgagccagca ttttgcggaa gtctttgata
    36481 ccggaggccg ccagagtacg gatcggacca gcagagatgg cgttaacacg cacaccttcc
    36541 ggacccatcg cgttcgccat atagcgcacg ttcgcttcca gagacgcttt tgccagaccc
    36601 ataacgttgt agttcgggat agcgcgctca gcgccaaggt aggaaagggt cagcagggca
    36661 gaacccggat tcagcatgga gcggcaagct tttgccattg caacgaagct gtaggagctg
    36721 atgtcgtggg caattttgaa gccttcacgg gtaacggcgt taacatagtc accatccagc
    36781 tgatcgccag gtgcaaaacc aatagagtgt acgaaaccgt caaatttcgg ccaaactttc
    36841 cccagttcag cgaacatggt gtcgatgctg gcatcttctg caacatcgca ctgcagaacg
    36901 atgtcagaac ccaattgagc ggcaaattct tctacgcggc ctttcagttt gtcgttctgg
    36961 taggtgaatg ccagttcagc tccttcgcgg tgcatcgcct gagcgatacc gtaggcgatg
    37021 gacagtttgc tggcaacacc ggttaccaga atgcgcttac cggaaagaaa acccatagct
    37081 ttaatcctta ttgttgatgc ttgttgtgcc tgaaaatcag gcgattcgtc gttttagtaa
    37141 acagtacgaa cagataaacg gttattataa tcaacctggc tgtgagtagc tatagttgcc
    37201 aggtccgacc ggagcaggct gcggcagggg ggcgcttttc cccttaccct aaccctctcc
    37261 ccagaggggc gaggggacca tattgtgcaa atattgttac cccagcaaca aacaggctca
    37321 tacagcccct aaccctttta tggcgatggc tgggatggtt cagaccttgc cgaatattct
    37381 ccagcactgt ctccatgttt tcaccacaac agctattcgg ctcggtcggc cccctcgccc
    37441 tttcagggtg agggtgaccg gcggttcagt tcctgcagaa taatctccag caccgcctcc
    37501 tcattacaat caatctcgtt attccagaaa cgcagcacgg tccagccctg cgactcaagc
    37561 cagctagtgc gcctggtatc ataggcaact gccaaatcat gctgcccacc atccagctca
    37621 acgactacgc gcgccgagca gcaagcaaaa tcgagaatgt agctccccac cggatgttga
    37681 cggcgaaatt tgaaatcacc aaaacgtcgg ctgcgaagat atcgccagag ctttcgttcc
    37741 tgcaaagtga gattgcgtct taaatcacgc gcatttgatt ttattttatc catcacctca
    37801 ttctgacaag atttaatctc ttgtcagaat gaggtgaata atctggaagg aggattcaga
    37861 aaattagcga tctttacgcc acgcatccgc tgtcaatgct tcgccaaagt gcccggcaat
    37921 cagccgtttg gtgagttcat gcagcggcga tgccagcaca tccgcggtgc tgcctcgctc
    37981 gacaacctcg ccctgatgca tcaccagcac ctggtcgcta atgtgcttca tcattccgat
    38041 atgctgggta acataaatat acgaaatgcc ctgtttttcc tgtaactcca gcatcagatt
    38101 aatcaactgc gaacgcattg acatatccag tgaagcgagg gcttcatcgg caataatgac
    38161 ttttgggcgc aatatcagcg cgcgcgccag acccagacgc tgtttttgtc cgggtgccaa
    38221 catatgcgga tagtaactga cgtgatccgg tagcagccca accatacgca tcgtttcaat
    38281 aatctgtttg cgacgctgtt ccggttccag atcggtgttc aggcgcagtg gaaaatccag
    38341 aatttgtgag atacgttgac ggggattcaa cgaggtcgaa ggatcctgaa aaatcatgcg
    38401 aatacgctga ctacggaagg aataatcgcc aaaatgcagc ggatgatcgt caatcaataa
    38461 ctcgccgctg gtaggttcta tcattcccgc cagcattttt gccagtgtgg atttacccga
    38521 accattctcg ccaataatcg ccagtgtctg gccttcacgt agcgtaaagc tcaagggttt
    38581 taccgcttct acggtctgac gacgaaacca accggtccgg tagcggaacg ttttacttaa
    38641 attacgcact tcaagcagcg tttcgatcat ctcacttttt ctccatgttc agcgggaaat
    38701 gacaggcata gagatgattt ttcgcccccg tcaaacgtgg cgtcacaatg cattctcgtt
    38761 gtgcatacgg gcaacgtggc cccagacgac aaccaatcgg taactgttcc agcaatggga
    38821 tagcgccggg tagagtattg aggcgacttt tatgcggcat cgcgctgccg aagtctggta
    38881 tcgcgcggat cagcgcctgg gtataaggat gatgtggcat cgtcaccaac tccttactcg
    38941 gcgcggtttc cactgtttga ccgcagtaaa gcacgttaat tttatccgcc cattggctaa
    39001 gcatttgtaa gtcatggctg ataagcaaaa tagtggtatt gctgttttgg ttgagacgcg
    39061 tcagcaggcg aaagatttgc gcctgggttg ttggctccat tgagtttgtc ggttcgtcag
    39121 caatcagcag acgcggttga ttcgccagtg caatggctat catcactttc tgacattcac
    39181 cttcggtcaa ctcataggga aaactgcgca tcgcatcttt gtgatcttta atccccacgc
    39241 ggtgcagcag ttcaatggca cggcgtttgc gccagccaaa acgctgccac caacggcctt
    39301 tataggtcca ggctgggatg ttttgcatca actggcgacc cacacgttct gaagggtcaa
    39361 gacacgactg cggttcctgg aaaatcatcg acacgttatg gccaaccagt ttgcgccgtt
    39421 cgcgtgcgga gagacgcagc aaatcgatat catcaaaacg catacggtca gcagtaacac
    39481 gccagttatc tttattcacc ccacaaatcg ctttcgcaat caaactcttg ccggaaccgg
    39541 attcaccaac aagaccgcgg atttcacctt cggttaaggt catacttacc cggtcgacgg
    39601 ctttaaccca ctcatcaccg gttttaaatt caatggtcag gttacgaata tcgagtaatg
    39661 gcattattcc acccccgcaa taatcgcacg acgaacgcca tcgccaagga ggttaaccaa
    39721 caacacgcta atcataattg ccgcacctgg cagcatgaca gtccacgggg cgacataaat
    39781 cagttccagc gcatcaccga gcatcgctcc ccattcaggc gaggggagtt gtgcgccgag
    39841 atcgagaaag cccagcgcgg cgatatcgag aattgccatc gacagtgcgc gggtgatctc
    39901 ggtgaccagc ccggcggtga tgtttggcat cacagcaaac cagagaatat tcagcgttga
    39961 tgcgccatcc agacgggcgg cgataacgta ctctttttcc agttcgtcat gcaccatgct
    40021 gtaaatcgaa cgtaccatac gcggcagcaa tgccagccag acagcaaaca tagcgtggga
    40081 caaactcggt ccggcaaacg ctacaacgat aattgccaac agcagcgaag ggatcgccag
    40141 taaggtatcc agaatatggt taagcaccgc tgagcggagg ccgtgtgtcg ccccggcgaa
    40201 ggtgcccagt accaggccac agatcgtcgc ggcaagcgtc accacaaatg cgccacccac
    40261 ggttggcgca gctccgctca gtaaacggct taaaacatca cgtccgaggt cgtcagtccc
    40321 caggaagaaa gaaacttcgc catagcgcga ccatgatggc ggcaataatt gataaccgag
    40381 aaattgctgg tcgatgccgt aaggcgcaaa ccagccgcca aaaatacaca gtacagccag
    40441 tcccgcgcag ccgtacaggc cgaccatcgc agaggcgtca ctataaaatt tgcgccaggc
    40501 ggtacgcagc gtgcccggcg ggcgcttttc gctgtatacg ctatcgtaag gcataccatt
    40561 ccttatgttt cagagggtta gccatggcac ccaaaatatc agaaatcacg ttaacaataa
    40621 tgaccagtga gccacacacc atcactccgg cggaaatggc tgcgtaatcc tgctggcgga
    40681 tggcgttaat taaccagcgc cctaaacccg gccagctaaa aaccatttcg gtgatcatcg
    40741 ccagcgtcag catggtggaa aactgtaagc ccaggcgagg aataaccggc ggtaacgcgt
    40801 tatgcagaac gtggcgacgc aaaatagtaa agcgcgacaa accgcgggtc gccgccgctt
    40861 ttacatagtt ctggtcatac acttcaatag tgctgatacg catcaggcgg atcacttcag
    40921 ttgttggcgc aaccgacagg gtgatcacgg gcaatatcat atggcggatt gcgctcatga
    40981 tcatttcatc ccgccatggt gagtccgaaa gccaggcatc aatcaacgca aaaccggtaa
    41041 tcggtttcac ttcgtagagc agatcgaaac gccctgaaac gggcaaccaa cctagcgtga
    41101 gtgaacaaaa cagtgtcaac agaagcgcca gccagaacac cgggattgaa aagcccagta
    41161 aggcgatggc gttgatcaag ttatcctgcc acttatggcg cgtaatcccg gcaatcatcc
    41221 ccacggggat gccaacaatc agcgcaaaac cgaaggcgag gatgcacagt tctatcgtcg
    41281 ccgggaagag ctcttttaac tgttcggcaa ttggttgacc gttaatgctg gaaacaccaa
    41341 aatcccagtg gatcaggcca ttaaaccaga acacccaggc attccacagt gacgcgcctt
    41401 gtaacggcgc gtgtggagtg aaatagctca agctgaagcc aacgaaggtc agcaggaaca
    41461 aggtgacaat caataacaaa atgcggcgta aggtgaagat aatcatggtt ttttcacctc
    41521 atcctgtttc tcgcgataca ccccagcaaa ggaggcgtta ccaaacgggc taagtaccag
    41581 acctttgata tcgtaccgat aggcctgcaa acgcaatgac gacgccagcg gcagaatagg
    41641 taattcctgc gccagaatac tctgcgcttc gtcataggct tcaatacgcg ctgccagctg
    41701 ctgcgaggag agcgccttac gcaacacgct gtcgaatttc ggatcgcacc agtgggcgag
    41761 gttggtctgt gaatgaattg ccgcgcagct cagtaacgga cggaagaaac tgtccgggtc
    41821 gttactgtcc gtcgcccaac cggataacgt cagatcatgg ctcatatcca tcaaccgcgc
    41881 ctcctgaaag cgaccttcta ccggcacaat caccactttt acgccaacct gcgccatatc
    41941 cgcctgaatc agttcggcag ttttcagtgg actggggttc cacgcctgcg aacgtgtggg
    42001 cacccacagt ttcagcgtta agttttccag ccccaacgcc ttcaactgtt cgcgcgattt
    42061 cgccggattg tattcagtaa ttttagcctc gttgtcatag gcccacgagg cgcgcggtaa
    42121 aatagaggcc gccgtttcag ccgtaccata atagatggat tgcatcaggc gctggttatt
    42181 aatcgccagt gccagtgcat ggcggacagc gggattattt agcggcggtt tggcggtgtt
    42241 aaatgccaga taggcgacgt tcatcccagg acgcagcgtt aaacgcaagc gcgggtcgtc
    42301 acgcaaaatg gatagctggc tggcagcagg ccaggccaga acgtcgcatt ccccggtcag
    42361 gagtttcgac agacgtccgg tgccgccgga gcctaaatcc accactacct gcggcattaa
    42421 cggtttaccg cgccagaagt catcatgacg ttgtaggcga ataaattgcc cggcgcggta
    42481 ttccgacaac tgatacggac cggtgccgac cggttgacgg tcgagttgct cctggcgatc
    42541 ttctttctct aacttccggg catattctgc cgacatgacc gaagcataat gggttgcgag
    42601 gtgccacaaa aaagaagcat ccggctgggc gagtcggaac tcaacggtat gattatccag
    42661 tttgcggacg cttttcacgt tatcggcaaa ttgcaggctg tcgaagtagg ggaagttgct
    42721 gccgttgacg ttatgccacg ggttgttgcg gtcaaaaatt cgctggaagg taaacaccac
    42781 atcgtcggca ttcattttac gagtgggagt aaaccagtcg gttttttgaa acggaacatc
    42841 gcgacgcagg tggaagcgat aggtcgcgcc gttgtcgagt acttcccagc tttcggcaag
    42901 ttccggcatc aggcgatagg tatagggatc gacatccagc agtcgatcat aaaactgggc
    42961 ggcaagggta tcgacaatta acccactgct cgctttggat gggttaaagg tgttgacttg
    43021 cccgctgacg caatagacaa aaccgctgtc gcggatatca gcatgcgggg gggattcagg
    43081 cgcggcgatt gcctgaccac tcacaagtcc agcaatcacc aaaagagacg ataatacctg
    43141 gcgcataata ttaagggatt ttatgtaaag aggctatctt actaatattt aatgacattt
    43201 gccattaccg tttgtgttca ggggtcgtaa tgagggccgt gtggttggtc gttgaacagg
    43261 tggcgtcgcc atctgttcaa cattcgtacc cgtgatttct ctataactat aactcacaga
    43321 acaacttagc gaggagcaga gcggtacgat cgtgagaaac ccattaaagc acgctgcagt
    43381 gcgtgtcatt gttagccgga tgcggcgtga acgctttatc cggacaacga tactgaccga
    43441 tcgtctgcaa ttaaataatt actcattacc ccattgattc aaaaactctg cgatctcatc
    43501 aatgcgtacg ggattaatcc ccgcttcagc agccatttca tgttgggctt cttcgctgat
    43561 ctcttcattg ttcatcaaac gggtgagcag taactggaag tagtgggcca gcggtgtgcg
    43621 ttcagcctct gcgacaggct ctgcgcattc ggtcgcgtac tcatcggcaa tatcaaaata
    43681 ttttagcggg atatcgtggt tcattatttg cccctggggt taatgcgcta agcataaggg
    43741 cggatgatag cactcgtgat ccgggtcata catcttttta tagttaatgg cgcggcgcgt
    43801 taccctcagg caggataatg cgccgcgcat ccggctatta cgtttcgctc accggcgttc
    43861 tgtcaccttc gtacagcggc actttgcgat agcggcggat cagataccac aaatatgcgc
    43921 cgcctaacga agcccatacc agaccgagtg tcagtgacgt tgactctagg ttaacccaca
    43981 gcacacccac cgtcagcgcg ccaaccagcg gcatcagcaa atagtggaag tgatccttcc
    44041 agcttttatt cattccttta cgccgccaga aatgattaaa caccgacaga ttcacgaagg
    44101 taaacgccac cagagcaccg aagttaatca gtgctgtcgc ggtgactaaa tcaaagaaca
    44161 gcgccgacag cgcgacaatc ccgaccataa tgacgttgag tgccggagtc cgccattttg
    44221 ggtgcacata gccaaacacg cgctccggaa acacattgtc acgccccatc acatacagca
    44281 gacgcgacac gctggcatgt gaagccaggc cagacgctaa tgtgttcaca aacgtggtgc
    44341 agaggaaaat cgactgaaag agcttgccgc cgacgtacaa cgcaatttca ggcagtgcgg
    44401 cgtccgggtc tttaaagcgg ctgatatcgg gaaagaacag ctgcataaag aacgacgccg
    44461 cgataaagat aacgccgccg tataccgccg tcaggaagat ggctttcggg atcacgcgtg
    44521 cggcgtccgg cgtctcttcc gaaagcgtgg tcaccgcatc gaaaccgagg aacgagaaac
    44581 agacaatcgt cgcaccggta ataatcggga tcaggtgcgc gttctcgctg ataaacggct
    44641 gaagtgacca gacggtgcca acgccttctc ctttatgcag tccctgaacc accaggaaga
    44701 taaacaccac catgatggag atttgcacca gtacaaacag ggtattgaag ttagcgacca
    44761 ggttgacgct cttcagattc gcggcggtta aaatggcgac gaaggttacc acccacaccc
    44821 acggcggcac ttccgggaag agggcggaga gatagatttt cgccaacaaa acgttgatca
    44881 ttggcaaaaa gagataatcc agcagtgatg accagccgac cataaatccg acgtgcgggt
    44941 taatcgactt ttgcgcgtag gtataggccg aaccggcctc cggaaactgg cgaaccagtt
    45001 tgccgtagct gatagcggta aacagcacgc ccgccagcgc cagcaaatag gacgccggaa
    45061 cgtgaccgtc gctaatgccg gagacaatac caaaagtatc aaatacggtc atcggcgtga
    45121 gataggccag acccatcatt accacctgcc acaatttcag tgattttcgc agacgggttt
    45181 tgccgggttg cgcagcaata ttcagtggtg aattaatagc cataattgct gcctcccctg
    45241 cgtaataagg tttgcggtcg tgtcgacctg aaatcaaacc tgtttagcaa gggaagcaaa
    45301 gggggcggag tccagtcagt agcaggaggg gcgcatacgc agcctccctc gccatgcggg
    45361 gatttaatca tcggcatcat ctcatttcct cgtcacagtc ttaatcgtta acggaagccc
    45421 gttgccgcct gcgctccata acgcgacatc cgggaaatgt ctggattacc agcaaatgtc
    45481 aggcctggct ccgctcaggc cgatgaaaca accccgcaag gggaattacg cgtttttcaa
    45541 catccactca atttctgttt ctgtgatgag gcgctcaaac tgcaacagct catcattttt
    45601 acaggcgtga tacacatggc aaaaccgttc gcctaaatag cggcgcaggt gatcattctc
    45661 gataaactca cccagggcat cgctctggcg aatcgggaag ggtaagcctt cctgttccag
    45721 cccgttgcct tcgacttctt cctgcaacgg cagctcgtta tcaaggccat gcaaaatacc
    45781 ggcaaaaatc gctgccatca ccagatatgg gttggcatcg gcacccgcca cgtgatattc
    45841 cacgcggtga ttatgacggt caccgcacgg aatacgcagg gcgacggtgc ggttgttatg
    45901 gccccacgac gcctgcgtcg gcacatacat tcccggctgg aagcggcgat acgagttcac
    45961 gtttggtgcc agcaacgcca tcgacgacgg catcaggtca atcatcccgg cgagcatctt
    46021 tttcagcagc ggcgaatctt cgccttctgt gtcagaaagc acattctcgc cacgattatt
    46081 ttgcatactg atatggatat gcatcccgct gcccgcgtgc tcttcatacg gcttcgccat
    46141 aaaagtggcg tgcatcttat gcttttctgc catcagacgc actaggcgtt ttagtgccag
    46201 cgcatcatcg caggcttcca gcacgttatc agtatggtaa aggttgattt caaactgacc
    46261 cggcgaggcc tcagcgaccg cgccatctgc cggaatcagc tgcaactgcg ccagttcatc
    46321 aatatcattg agcacgtcgg cgaagtggtt gaggttatca acggagtaaa cctggctttg
    46381 tgtattgcgg tcatcggtgc caggcgcgca gggcggttgc agatcccctt cagcgtcgcg
    46441 ctggcgatcc agtaaataga actccagctc taccgctaca accgggaaca atccgcgctg
    46501 gcgcagctgc tgccacaggc ggttaagaac gttccgcggc tcaacgtcaa agggagcgcc
    46561 atcttcatcg accatggtca ggagcatctg accgataaac tctggatcgg cggcagaagg
    46621 agttaaggaa ccgagaacag gaacacaggt acgatccggc tcgcccattt cctgacccag
    46681 acctgcttct tctactacgt tgcccagaat atccattgca aataccgagg ccgggaaata
    46741 acaccctttc tcgagcttct tcaggcttga aacagggata cgttttccac ggaagcaacc
    46801 attcaaatca gtaagtaaaa catcaacata ttgcgtattc gggtagcgct ccaggtagcg
    46861 tttcacttcc tgcgtaaagg cgctaccccg cctctcttct gactgctgaa caaagttctc
    46921 tacttcaacg atattggttt ccatgattct tcgcctttgg tttgttttcc gctcgttatc
    46981 aaagcgtaaa atataatgac catcattcga atctgtatgc aaaccaaatg tttgttaaat
    47041 gttaaattga gtttgcaaaa atgaaaatcc actgctagat tgaaaaaata ttgaacataa
    47101 aggtcattta aagcgcagta atggcgataa tttagtccac tttgtgagat tgagcatgga
    47161 aaatataatg aacaatccgg ttatcggtgt cgtaatgtgc aggaacaggc ttaagggtca
    47221 tgcgacccag actctgcaag aaaagtacct gaatgccatc atccacgcag gcggcttgcc
    47281 aattgcgcta ccacatgcgc tggcggaacc gtcgttactt gaacaacttt tgccgaaact
    47341 cgatggcatt tatcttccta gtagtcccag caatgtgcag ccgcacctat atggtgaaaa
    47401 cggcgatgag cctgacgccg atcccgggcg tgatcttctg agcatggcga taattaatgc
    47461 cgcactcgaa aggcgcatcc ccattttcgc catctgccgg ggtttacaag aactggtggt
    47521 ggcaaccggt ggttcgttgc atcgcaagct gtgcgaacag cctgaattgc tggaacatcg
    47581 ggaagatccc gaactgccgg tggaacagca atatgcaccg tcgcatgaag ttcaggttga
    47641 agagggggga ttactgtctg cgttgttacc tgaatgtagc aacttttggg taaactctct
    47701 acatggacaa ggggcgaagg tcgttagccc acggttgcgt gttgaagctc gctcgccgga
    47761 tggcttagtt gaggcggtta gcgtcatcaa tcatcctttt gcgctgggcg tacagtggca
    47821 cccggaatgg aacagtagcg agtacgcgct ttcgcgtata ttgttcgagg gctttatcac
    47881 cgcttggcag caccatatcg ctgaaaaaca gcgactctga ccactacagt ttaaggaaat
    47941 gcaaatatga gtgatgaggg actggcgcca ggaaaacgct tgtcggaaat ccgccagcag
    48001 caggggcttt cacaacgtcg tgccgccgaa ctctccgggc tgactcacag tgctatcagt
    48061 acgatagaac aagataaagt cagccctgcc atcagtacgc tgcaaaagct gctgaaggtg
    48121 tatggtctgt cactctcgga attcttttcc gagccggaaa aacctgatga gccgcaggtc
    48181 gtcattaatc aggacgactt aattgagatg ggcagtcagg gtgtgtcaat gaagctggtt
    48241 cataacggta acccgaatcg cacgctggcg atgatctttg aaacgtacca gccgggcaca
    48301 accactgggg aaagaattaa gcatcagggt gaggaaatag gcacagtact ggaaggtgaa
    48361 attgttctga cgattaatgg tcaggattac cacctcgtcg cggggcaaag ttatgccatt
    48421 aataccggca tcccgcacag tttcagtaat acgtcggcag gtatttgccg aattatcagc
    48481 gcccatacgc ccaccacgtt ttaatctttt tgttctgtaa gccgggtaag cgcaatgcgc
    48541 ccggcaatcc ataaagaatg tcgccagaaa taaacgggca tgcagcccgg ggatctctgc
    48601 gccctgacgt tcacaaactg catatatctg atagacgtga aacaggagtc ataatgaatt
    48661 ttcatcatct ggcttactgg taggataaag cgttaagtct cgccattgaa aaccgcttat
    48721 ttattaacgg tgaatatact gctgcggcgg aaaatgaaac ctttgaaacc gttgatccgg
    48781 tcacccaggc accgctggcg aaaattgccc gcggcaagag cgtcgatatc gaccgtgcgg
    48841 tgagcgcagc acgcggcgta tttgaacgcg gcgactggtc actctcttct ccggctaaac
    48901 gtaaagcggt actgaataaa ctcgccgatt taatggaagc ccacgccgaa gagctggcac
    48961 tgctggaaac tctcgacacc ggcaaaccga ttcgtcacag tctgcgtgat gatattcccg
    49021 gcgcggcgcg cgccattcgc tggtacgccg aagcgatcga caaagtgtat ggcgaagtgg
    49081 cgaccaccag tagccatgag ctggcgatga tcgtgcgtga accggtcggc gtgattgccg
    49141 ccatcgtgcc gtggaacttc ccgctgttgc tgacttgctg gaaactcggc ccggcgctgg
    49201 cggcgggaaa cagcgtgatt ctaaaaccgt ctgaaaaatc accgctcagt gcgattcgtc
    49261 tcgcggggct ggcgaaagaa gcaggcttgc cggatggtgt gttgaacgtg gtgacgggtt
    49321 ttggtcatga agcggggcag gcgctttcgc gtcataacga tatcgatgcc atcgccttta
    49381 ccggctccac gcgtacgggt aaacaactgc tgaaagacgc gggtgatagc aacatgaaac
    49441 gtgtctggct ggaagcgggc ggcaaaagcg ccaacatcgt tttcgccgat tgtccggatc
    49501 tgcaaaaagc ggcaagcgcc accgccgcag gcatcttcta caaccagggc caggtgtgca
    49561 tcgccggaac gcgtttgtta ctggaagaga gcatcgccga tgaattctta gccctgttaa
    49621 aacagcaggc gcaaaactgg cagccgggcc atccacttga tcccgcaacc accatgggca
    49681 ccttaatcga cagcgctcac gctgactcgg tacacacctt tattcgtgaa ggcgaaggca
    49741 aggggcaact gctgctggat ggtcgcaacg ccgggctggc agcggccatc ggcccgacca
    49801 tcttcgtgga tgtggacccg aatgcgtcct taagccgcga agagattttc ggtccggtgc
    49861 tggtggtcac ccgtttcaca tcagaagatc aggcgctgca gcttgccaac gacagccagt
    49921 acggccttgg cgcggcggta tggacgcgcg acctctcccg cgcgcaccgc atgagccgac
    49981 gcctgaaagc cggttccgtc ttcgtcaata actacaacga cggcgatatg accgtgccgt
    50041 ttggcggcta taagcagagc ggcaacggtc gcgacaaatc cctgcatgcc cttgaaaaat
    50101 tcactgaact gaaaaccatc tggataagcc tggaggcctg aaatgaccga acataccagc
    50161 agttactacg ccgccagtgc gaataaatat gcaccattcg acacactgaa tgagtcaatc
    50221 acctgcgacg tttgcgtggt tggcggcggc tataccgggc tctcctccgc gctgcatctg
    50281 gcggaagcgg gctttgacgt agtggttctc gaagcctcac gcatcggctt tggcgcaagc
    50341 gggcgcaatg gcgggcagct tgtgaactcc tacagccgcg acatcgacgt gatcgaaaaa
    50401 agctacggca tggacaccgc ccgtatgctc ggcagcatga tgttcgaggg tggcgagatc
    50461 atccgcgaac gtatcaaacg ttatcagatt gactgcgact accgccccgg cggtctgttt
    50521 gtggcgatga atgataaaca actcgccaca ctcgaagagc agaaagagaa ctgggaacgc
    50581 tacggcaata aacagctgga gctgctggac gtcaacgcta ttcgccgcga ggtcgccagc
    50641 gatcgctaca ctggtgcgct gctggatcac agcggtgggc atattcaccc gctaaacctc
    50701 gccattggtg aagccgatgc catccgcctg aacggcgggc gcgtgtatga gctttccgcc
    50761 gtgacgcaga tccagcacac cacgccagcc gtggttcgga ctgccaatgg tcaggtgacg
    50821 gcgaagtatg tgattgtcgc cgggaatgcg tatctgggcg ataaagtaga gccggaacta
    50881 gcgaaacgca gcatgccgtg cggcacgcag gtgatcacca ccgaacggct gtcggaagat
    50941 ttagcccgtt cgctgatccc gaaaaactac tgtgtggaag actgtaacta tctgcttgat
    51001 tactaccgtc ttaccgccga caaccgcctg ctgtacggcg ggggtgtagt gtatggtgca
    51061 cgcaacccgg atgatgttga gcgtcttgtg gtaccgaaac tactaaaaac cttccctcag
    51121 ttgaagggcg tgaaaattga ttaccgctgg acgggcaact tcctgctgac cttgtcgcac
    51181 atgccgcagt tcggccgcct tgataccaac atctattaca tgcagggtta cagcggccac
    51241 ggcgtcacct gtacccatct tgccgggcgt ttgattgctg aactgctgcg cggtgacgcc
    51301 gagcgtttcg atgccttcgc caatctgccg cattacccgt tccccggcgg gcgcacgctg
    51361 cgcgtgccat ttaccgcgat gggcgcggct tattacagcc tgcgcgatcg tctgggcgtt
    51421 taatttccga ttaacagtga agagtcaaaa ggtgtgaaac atgagcaaca atgaattcca
    51481 tcagcgtcgt ctttctgcta ccccacgcgg ggttggcgtg atgtgtaact tcttcgccca
    51541 gtcggcagaa aacgctacgc tgaaggacgt agagggcaac gaatacatcg atttcgccgc
    51601 aggcattgcg gtgctgaata ccgggcatcg ccaccctgat ctggtcgcgg cggtggagca
    51661 gcaattgcaa cagtttaccc acacggcgta tcagattgtg ccgtacgaaa gctacgtcac
    51721 gctggcggag aaaattaaca cccttgcccc agtgagcggg caggctaaaa ccgcattctt
    51781 cactaccggc gcggaagcgg tggaaaacgc ggtgaaaatt gctcgcgccc ataccggacg
    51841 acctggcgtg attgcgttta gcggtggctt ccacggtcgt acatatatga ccatggcgct
    51901 gaccggaaaa gttgcgccgt acaaaatcgg cttcggcccg ttccctggtt cggtgtatca
    51961 cgtaccttat ccgtcagatt tacacggcat ttcaacacag gactccctcg acgccatcga
    52021 acgcttgttt aaatcagaca tcgaagcgaa gcaggtcgcg gccattattt tcgaaccggt
    52081 gcagggcgag tgcggtttca acgttgcgcc aaaagagctg gttgccgcca ttcgccgcct
    52141 gtgcgacgag cacggtattg tgatgattgc cgatgaagtg caaagcggct ttgcgcgtac
    52201 cggtaaactg tttgccatgg atcattacgc cgataagccg gacttaatga cgatggcgaa
    52261 aagcctcgcg ggcgggatgc cgctttcggg cgtg
//
LOCUS       DAJVPP010000014        49124 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVPP010000014 DAJVPP010000000
VERSION     DAJVPP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.014"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..811
                     /locus_tag="OQM34_000834"
     CDS             <1..811
                     /locus_tag="OQM34_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1451387.1"
                     /translation="ANYHTTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGI
                     QPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNTKGMSDPIQMLRALTGDVGF
                     DDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKVPFNFYNVHYNSTHVVGTSG
                     GSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPL
                     TAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            808..1773
                     /gene="yggF"
                     /locus_tag="OQM34_000835"
     CDS             808..1773
                     /gene="yggF"
                     /locus_tag="OQM34_000835"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1451388.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            1795..2304
                     /gene="fumE"
                     /locus_tag="OQM34_000836"
     CDS             1795..2304
                     /gene="fumE"
                     /locus_tag="OQM34_000836"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCS1451389.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2301..3014
                     /locus_tag="OQM34_000837"
     CDS             2301..3014
                     /locus_tag="OQM34_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1451390.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(2986..3663)
                     /locus_tag="OQM34_000838"
     CDS             complement(2986..3663)
                     /locus_tag="OQM34_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1451391.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(3657..4334)
                     /locus_tag="OQM34_000839"
     CDS             complement(3657..4334)
                     /locus_tag="OQM34_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1451392.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4322..4705)
                     /locus_tag="OQM34_000840"
     CDS             complement(4322..4705)
                     /locus_tag="OQM34_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1451393.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(4721..5029)
                     /locus_tag="OQM34_000841"
     CDS             complement(4721..5029)
                     /locus_tag="OQM34_000841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451394.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5030..5608)
                     /locus_tag="OQM34_000842"
     CDS             complement(5030..5608)
                     /locus_tag="OQM34_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1451395.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5631..6062)
                     /locus_tag="OQM34_000843"
                     /pseudo
     CDS             complement(5631..6062)
                     /locus_tag="OQM34_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6434..7453
                     /gene="epd"
                     /locus_tag="OQM34_000844"
     CDS             6434..7453
                     /gene="epd"
                     /locus_tag="OQM34_000844"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1451396.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLFLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7503..8666
                     /gene="pgk"
                     /locus_tag="OQM34_000845"
     CDS             7503..8666
                     /gene="pgk"
                     /locus_tag="OQM34_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1451397.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8881..9960
                     /gene="fbaA"
                     /locus_tag="OQM34_000846"
     CDS             8881..9960
                     /gene="fbaA"
                     /locus_tag="OQM34_000846"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1451398.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10151..11011
                     /gene="mscS"
                     /locus_tag="OQM34_000847"
     CDS             10151..11011
                     /gene="mscS"
                     /locus_tag="OQM34_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1451399.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11150..11785
                     /gene="argO"
                     /locus_tag="OQM34_000848"
     CDS             11150..11785
                     /gene="argO"
                     /locus_tag="OQM34_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1451400.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11878..12618
                     /locus_tag="OQM34_000849"
     CDS             11878..12618
                     /locus_tag="OQM34_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1451401.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12785..13735
                     /locus_tag="OQM34_000850"
     CDS             12785..13735
                     /locus_tag="OQM34_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1451402.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(13677..15155)
                     /gene="scpC"
                     /locus_tag="OQM34_000851"
     CDS             complement(13677..15155)
                     /gene="scpC"
                     /locus_tag="OQM34_000851"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCS1451403.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15179..15964)
                     /gene="scpB"
                     /locus_tag="OQM34_000852"
     CDS             complement(15179..15964)
                     /gene="scpB"
                     /locus_tag="OQM34_000852"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1451404.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(15975..16970)
                     /gene="meaB"
                     /locus_tag="OQM34_000853"
     CDS             complement(15975..16970)
                     /gene="meaB"
                     /locus_tag="OQM34_000853"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1451405.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(16963..19107)
                     /gene="scpA"
                     /locus_tag="OQM34_000854"
     CDS             complement(16963..19107)
                     /gene="scpA"
                     /locus_tag="OQM34_000854"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCS1451406.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19311..20204)
                     /gene="argP"
                     /locus_tag="OQM34_000855"
     CDS             complement(19311..20204)
                     /gene="argP"
                     /locus_tag="OQM34_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1451407.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20587..21246
                     /gene="rpiA"
                     /locus_tag="OQM34_000856"
     CDS             20587..21246
                     /gene="rpiA"
                     /locus_tag="OQM34_000856"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1451408.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21502..22734
                     /gene="serA"
                     /locus_tag="OQM34_000857"
     CDS             21502..22734
                     /gene="serA"
                     /locus_tag="OQM34_000857"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1451409.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22963..23022
                     /gene="ibsC"
                     /locus_tag="OQM34_000858"
     CDS             22963..23022
                     /gene="ibsC"
                     /locus_tag="OQM34_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1451410.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23355..23414
                     /locus_tag="OQM34_000859"
     CDS             23355..23414
                     /locus_tag="OQM34_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1451411.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23515..24117)
                     /locus_tag="OQM34_000860"
     CDS             complement(23515..24117)
                     /locus_tag="OQM34_000860"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1451412.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRASKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24138..24321)
                     /gene="ssrS"
                     /locus_tag="OQM34_000861"
     ncRNA           complement(24138..24321)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQM34_000861"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24363..24692)
                     /gene="zapA"
                     /locus_tag="OQM34_000862"
     CDS             complement(24363..24692)
                     /gene="zapA"
                     /locus_tag="OQM34_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1451413.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24860..25438
                     /gene="ygfB"
                     /locus_tag="OQM34_000863"
     CDS             24860..25438
                     /gene="ygfB"
                     /locus_tag="OQM34_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1451414.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25464..26789
                     /gene="pepP"
                     /locus_tag="OQM34_000864"
     CDS             25464..26789
                     /gene="pepP"
                     /locus_tag="OQM34_000864"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1451415.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            26786..27964
                     /gene="ubiH"
                     /locus_tag="OQM34_000865"
                     /gene_synonym="visB"
     CDS             26786..27964
                     /gene="ubiH"
                     /locus_tag="OQM34_000865"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1451416.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            27987..29189
                     /gene="ubiI"
                     /locus_tag="OQM34_000866"
                     /gene_synonym="visC"
     CDS             27987..29189
                     /gene="ubiI"
                     /locus_tag="OQM34_000866"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1451417.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29263..29346)
                     /locus_tag="OQM34_000867"
     CDS             complement(29263..29346)
                     /locus_tag="OQM34_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451418.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29637..30731
                     /gene="gcvT"
                     /locus_tag="OQM34_000868"
     CDS             29637..30731
                     /gene="gcvT"
                     /locus_tag="OQM34_000868"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1451419.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTWEFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            30755..31144
                     /gene="gcvH"
                     /locus_tag="OQM34_000869"
     CDS             30755..31144
                     /gene="gcvH"
                     /locus_tag="OQM34_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1451420.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31263..34136
                     /gene="gcvP"
                     /locus_tag="OQM34_000870"
     CDS             31263..34136
                     /gene="gcvP"
                     /locus_tag="OQM34_000870"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1451421.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34403..35146
                     /locus_tag="OQM34_000871"
     CDS             34403..35146
                     /locus_tag="OQM34_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1451422.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35203..36636)
                     /gene="bglA"
                     /locus_tag="OQM34_000872"
     CDS             complement(35203..36636)
                     /gene="bglA"
                     /locus_tag="OQM34_000872"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS1451423.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            36681..36991
                     /locus_tag="OQM34_000873"
                     /pseudo
     CDS             36681..36991
                     /locus_tag="OQM34_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37155..37814
                     /locus_tag="OQM34_000874"
     CDS             37155..37814
                     /locus_tag="OQM34_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1451424.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38010..38990)
                     /gene="ygfZ"
                     /locus_tag="OQM34_000875"
     CDS             complement(38010..38990)
                     /gene="ygfZ"
                     /locus_tag="OQM34_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1451425.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39233..39499
                     /gene="sdhE"
                     /locus_tag="OQM34_000876"
     CDS             39233..39499
                     /gene="sdhE"
                     /locus_tag="OQM34_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1451426.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39480..39887
                     /locus_tag="OQM34_000877"
     CDS             39480..39887
                     /locus_tag="OQM34_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCS1451427.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(39927..40448)
                     /gene="fldB"
                     /locus_tag="OQM34_000878"
     CDS             complement(39927..40448)
                     /gene="fldB"
                     /locus_tag="OQM34_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1451428.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40560..41456
                     /gene="xerD"
                     /locus_tag="OQM34_000879"
     CDS             40560..41456
                     /gene="xerD"
                     /locus_tag="OQM34_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1451429.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41481..42191
                     /gene="dsbC"
                     /locus_tag="OQM34_000880"
     CDS             41481..42191
                     /gene="dsbC"
                     /locus_tag="OQM34_000880"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1451430.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42197..43930
                     /gene="recJ"
                     /locus_tag="OQM34_000881"
     CDS             42197..43930
                     /gene="recJ"
                     /locus_tag="OQM34_000881"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1451431.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLLGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44021..45119
                     /gene="prfB"
                     /locus_tag="OQM34_000882"
     CDS             join(44021..44095,44097..45119)
                     /gene="prfB"
                     /locus_tag="OQM34_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1451432.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45129..46646
                     /gene="lysS"
                     /locus_tag="OQM34_000883"
     CDS             45129..46646
                     /gene="lysS"
                     /locus_tag="OQM34_000883"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1451433.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(46689..47237)
                     /gene="idi"
                     /locus_tag="OQM34_000884"
     CDS             complement(46689..47237)
                     /gene="idi"
                     /locus_tag="OQM34_000884"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1451434.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47292..47363
                     /locus_tag="OQM34_000885"
     CDS             47292..47363
                     /locus_tag="OQM34_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451435.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47360..47485)
                     /locus_tag="OQM34_000886"
     CDS             complement(47360..47485)
                     /locus_tag="OQM34_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451436.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47487..48935)
                     /gene="uacT"
                     /locus_tag="OQM34_000887"
     CDS             complement(47487..48935)
                     /gene="uacT"
                     /locus_tag="OQM34_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1451437.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11852 a        12323 c        13241 g        11708 t
ORIGIN      
        1 tgccaattat cacaccacgc aatatgttta tgagcatcgc atgggcgtca aacctggcgg
       61 caatattgca ctgctggcgt gtgcgggtcc gatgggcatt ggcgctatcg attacgccat
      121 taacggtggc atacaaccgt cacgggtggt ggtggtcgat atcgacgaca aacgcctggc
      181 gcaggtgcag aagctgctgc cggtggatct ggcggccagt aaaggcattg agctggtgta
      241 tgtgaatacc aaagggatga gcgatcctat ccagatgctg cgggcgctga caggagatgt
      301 cgggttcgat gacatttttg tttatgcggc ggtgcctgct gtcgttgaga tggctgacga
      361 attactggcg gaagatggct gtctgaactt ctttgccggg ctgacggata aaaacttcaa
      421 agtgccgttt aatttctaca acgtccatta caacagcacg cacgtagtcg gtacatccgg
      481 cggttcaacg gacgacatga aagaggcgat tgcccttagc gccacaggac agttacagcc
      541 gtcgtttatg gtgacccata ttggtggcct ggatgcggtg ccagaaaccg tgctcaatct
      601 gccggatatc cctggcggta aaaaactcat ttataacggc gtgaccatgc cgctcactgc
      661 cattgccgat tttgccgaaa aaggcaaaac cgatccgctg tttaaagagt tggcgcggct
      721 ggttgaggaa acgcacggca tctggaatga acaggccgag aaatatctgc tggcacaatt
      781 tggcgttgat atcggggagg ccgcgcaatg atgtccctgg cgtggccatt atttcgcgtt
      841 acggaacagg cagcgttggc tgcctggccg caaaccggat gtggcgacaa aaataaaatt
      901 gatggcctgg cggtcaccgc gatgcgccag gcattaaacg acgtcgcttt tcgtggacga
      961 gtggttatcg gcgaaggaga aattgaccat gcgccgatgc tgtggattgg cgaagaggtg
     1021 ggcaaaggcg atgggccaga agtcgatatc gcggttgatc ccattgaagg cacgcggatg
     1081 gtggcgatgg ggcagagcaa tgcgctggcg gtcatggctt tcgctcctcg agatagcctg
     1141 ttacatgcgc ccgatatgta tatgaaaaag ctggttgtta atcgactggc tgccggggcg
     1201 attgatctgt ctttgcctct ggcggacaac ttgcgtaacg tggcgcgggc gttaggtaaa
     1261 ccgctggaca agctgcgcat ggttacgctg gataaaccgc gtctgagtgc cgcgattgaa
     1321 gaagcaacac agctgggcgt gaaggttttt gccctgccgg atggcgatgt cgccgccagc
     1381 gtgctgacct gctggcagga taatccttac gatgtgatgt acaccatcgg cggtgcgcca
     1441 gaaggcgtga tttctgcctg tgccgttaaa gcgttaggcg gggatatgca ggccgagctg
     1501 attgactttt gtcaggctaa aggggattac acggaaaatc ggcagattgc agagcaggag
     1561 cgcaagcgtt gtaaggcaat gggcgttgac gttaaccgtg tgtactcgct cgatgaactg
     1621 gtgaggggga acgatatcct ctttagcgct acgggcgtga cgggcggcga gttggtgaac
     1681 ggtatccaac agacggcgaa tggggtgcgg acgcagacat tactgatcgg cggcgcggac
     1741 caaacgtgta atataataga ctccctgcat tgatggtgat ctatcggaag accaatggcg
     1801 acgctgacag aagatgatgt gcttgagcaa ctggatgcac aggacaattt attttcattt
     1861 atgaaaactg cgcattctat tttgctccag gggatacgcc agtttctgcc gtcgctgttt
     1921 gtcgataacg atgaagagat cgtcgaatat gcagtgaagc cgttactcgc ccaaagcggc
     1981 ccgcttgacg atattgatgt tgcgctgcgt ttgatttatg cgctggggaa aatggataaa
     2041 tggctgtatg ccgatatcac gcacttttcc cagtactggc attacctgaa cgaacaggat
     2101 gaaacgcccg gatttgccga tgatataacc tgggatttta tcagcaatgt caacagtata
     2161 actcgcaatg cgacgctcta cgatgcgtta aaagcgatga agttcgccga tttcgccgtc
     2221 tggtcagagg cgcgttttag cggaatggtc aaaacggcgc tgacgctggc agtaacgacc
     2281 accttaaagg aattaacgcc gtgaaaattg aattaacggt gaatgggctg aacgtgcaag
     2341 cgcagtacca tgatgaagag atagaacgcg tgcataaact gttgctgcgt atgctggcgg
     2401 ccttacagac ggtaaatccg cagcggcgta cggtggtttt tctttgcgct ccgccgggga
     2461 cgggtaaatc taccctaacc accttctggg aatatctcgc gcaacaagac ccggagctgc
     2521 ccgctattca ggcgctcccg atggatggtt ttcaccatta caatagctgg ctggacgcgc
     2581 atcaattgcg ccccttcaaa ggcgcaccag agacatttga cgttgcgaaa ctggcggaaa
     2641 atctgcgcca ggtggtggaa ggggattgta cgtggccgca gtacgatcga caaaagcatg
     2701 atcctgttga agatgcgttg cacgttaccg caccactcgt catcgtcgaa ggaaactggt
     2761 tgttgctgga cgacgagaag tggtgccagc ttgcgcaatt ttgtgatttc tcgatcttta
     2821 tcaacgcgcc agctgctgcg ttgcgggagc gtctggtggg gcgcaagctg gcgggagggg
     2881 tatcgctggc agatgcagaa gccttttatg accgtaccga cgggccgaat gttcgccggg
     2941 tgctggaaga gagtctgcct gcaaatttga ccttaatgat gaccgctacg ggtgaatatc
     3001 gtctggtgga ttaacgttcc tgattacacc atcttccagc cgcaccacac gagagaaatg
     3061 acgccgggta aatgcggcgt catgactgat tgctaccacg ctcgtgcctc gctggcggca
     3121 cttctccagc cagctttcaa acacgctcaa ccaattttcg tcaaaatccc gactcggctc
     3181 gtccagcagt aatagtggcg gagagagtgc ttccaggcag gcgacggcga ccatccgtcg
     3241 ctgcgcacta tgcagatcca acgggtgcga gttggcagtg tctgctaact gacaacattg
     3301 cagggcagca tgggtacgtt gagtgatttc atccgccggg catttttgca gtttcaaacc
     3361 aaatgcgatc tcgtcagcga cggtgctgtg aaaaagttgg ttttccgctt cctgaaacag
     3421 tacgccaacc tttgccgcgc gctggcggtt tttcaggttt ttcatcgcct gttgttgcag
     3481 cataaccgta ccggctgtgg gcgtaagaag acctgccatt actcgtaaca gcgtcgattt
     3541 ccctgcgcca ttgtcaccgg tcagcgccaa ccattcacct tgtttgagtt gcagagaaat
     3601 gtcgcaaaga caatccgttg ccgcgccagg ccagcgataa gatatcttat ttaacgttaa
     3661 catagtggct ggagcgctcc gttttgcaat tgccacgctt gctggcagta gttcaggaaa
     3721 ggtgtgcgat gacgttcaaa aagaataatc agcgaaccac gttcaaacgc ccagtgttgt
     3781 aagcgctgca acagcatttc gctggcctgc ggcgtcaggc ggctaaaggc ttcatcaaga
     3841 attaagagct ttggctgcat cgcgatggcg caggcgatga tcacgcgctg tgtctctcca
     3901 ccggagagtg ttgccgggtg acgatggcgc aacggctggc actcggtcag agtcagggcg
     3961 gcatcaatcc gcgccataat ctctgcttca gcaagacaca gattttctgg cccaaaggca
     4021 acttcttctt ccacgctaaa ggtacagccg gagagctgca aatagggcga ttgctggacg
     4081 agctggatgg tggcggattg ttcattaagc gggagatgcc caatgggcgt accgaggagc
     4141 gtccccgtgc ctgttatctc tcttggcaga aagtccggat accagcccgc catcagctgt
     4201 gccagcgtac ttttgccact gccgttgtca ccaaagatgg cgaccatccc cggtttgaca
     4261 tagtgaaagt cataacagaa cggaggatgc gttgagtgtg tcgggcaata acggaactgt
     4321 tctaacgtaa ccatatccac gctccgaatt ctgtcagcat cagcaagatc atggtgtaac
     4381 gcgctacgcg ttgtagggtg ctatcggctg gtgcccacaa cgttgttcgt cggttgtgga
     4441 tccgaaatgc ccgcatatcc agcgccgcac cacggacagc cagatcgtta agcgcattgt
     4501 gtgttagcgg aataattaac gcgggcatag cacgtaaacg ctgataccag ccctcatcca
     4561 gcggcacgcc gcgggcacgc tgcgcttcat gaataatcgc taattgccgt tttaactgct
     4621 caacgaccaa taacggcccg gcaaacagat aagcgacgcc tggcggcagg cgagaagcaa
     4681 acagagcgcg aataaaacat tgcacgggga cgtactgcat tcacaactgt gaggttgaaa
     4741 caatcgccag aatcagcaac cagagcgtaa tagcatgcga ccaccgttct ggcgagcgtg
     4801 gcgtgccgct tagccattct ggtagccagc caccgtgtac cagccacagc ccggctccca
     4861 gcgagaacat cagccagacg acatattttg cccgccgccg cgtggccttt agcgcaataa
     4921 ggcagaaaaa ggtcgccgcg ctatagatcg gcaaaatggt ctgcgcgggc aggataagcg
     4981 tggtgcaggc cgccaatgcc cacagtgtta atgaggtaaa tggatgcatt tagcgcacgg
     5041 cagccattgc cggaaaatgg cgcgtggtgc gaatcggcag ctggcgcagt aaaagccaga
     5101 caatcaccgc tgtaagaatt ttatcgacca ggttcgcgcc tatcaccgta atcgccaccg
     5161 actccaccag gttttgcccc atcgagtgca tccaggcaac gaacaaatcg gctccgctac
     5221 cggtgacacc gccgaacagc gcagtacgca gcggtacagc gacgaccgtt accgcaagcg
     5281 taataatcac gccgctgaca acgacttttg gcagggtgcg aaaccaaccc gctcgcgcca
     5341 gccaaccgga gaccaggccg atgaccatcg cgacgggtgc aaatgcggcg gcgatcgggt
     5401 cggtcagcaa tccccagagt aaattggtca gtaagccggt cagcattccg atcaccggac
     5461 caagcagtac cgcgctaata agtgtgccga tggagtcgag aaatataggt aatttgacca
     5521 tgctggccag ttgcccgccg atcatattga tggcgataga tataacgatc aacaccagtg
     5581 cctgactgga aaagtggcta cgcgccataa gaaaacctct ttgaaagtag ttatttaacg
     5641 ggggaaatat gcaactcgtc gtaaccagaa agcgcgcaag gttggcggct aaacgccagt
     5701 tcctcaaggc taccgatcac cagttcaagc gtggtccgca cggtacagcc gggcatcata
     5761 tgaccgccaa gcatcgtgcc gtgtggatcg gaaacgcaga gatgaagatg ctcaccactc
     5821 tgttccagcg tgccgtttag tgagatcact tcgaatgtac cgttcaggag cgtggtgcct
     5881 tcttgcccgg catagcgcaa ggcgacgtca gtcaggctac cggtacagcc tgcaatctag
     5941 gcggcatgaa gttgatgttg ctgtgcaaaa gcgtgtagct gggaaaaaac ttcctgcccg
     6001 gggaggaggc gcagtgcgta aaagcgtgcg gaggaagaat atgagtaggg caatgtcatc
     6061 attggagtac tccgaaactc ggacgtattg cggataatat cgacgctgca tcagtcatat
     6121 tctgatataa gtcagggttt gtgacggcac aacgaaaaga ttttgctacg cttattcgtg
     6181 attctgcaaa gtggaataaa ttccacaggg gcggggctga aaacgtgacg taagtcagat
     6241 aattgctttc tttgactgga caatcattcc ttttattcca cgtttcgctt atcctagctg
     6301 aagcgtttca gtcgattaaa tgttcgacaa ttaaccaatc agtcgcagtt tgcgacaggt
     6361 aaggtttccc cggacgattt gctggattac tctgtctggc aaatttgctc agggaaaacc
     6421 ttgcaggaga tctatgaccg tacgcgtagc gataaatggc ttcggtcgca tcgggcgtaa
     6481 tgtggttcgt gctttgtatg aatccggacg ccgggcggaa attaccgtgg tggcaatcaa
     6541 cgaactggcg gatgctgcgg gcatggcgca tttgttgaaa tatgacacca gccatggccg
     6601 ttttgcatgg gaagtacgac aggaacgcga tcaacttttt gttggtgatg acgccatccg
     6661 cgtattgcat gaacgttcac tgcaatcgct cccctggcgt gaacttggcg ttgatgtagt
     6721 cctcgactgc accggcgtat atggctcccg cgagcatggc gaagcgcata ttgccgccgg
     6781 ggccaaaaaa gtgctctttt cacatcctgg cagtaacgat ctcgacgcga ccgttgttta
     6841 cggcgtcaat caggatcaac ttcgtgcgga acaccgcatc gtttctaacg cttcctgtac
     6901 cacgaattgc ataattcccg tcatcaaatt gttagatgat gcgtacggta ttgagtccgg
     6961 cactgtgacc acaattcact ccgccatgca cgatcaacag gttattgatg cataccatcc
     7021 tgacctgcgt cgcacccggg cagccagcca gtcgatcatt ccggtcgata ctaaactggc
     7081 cgctggtatc acacgatttt ttccgcaatt taacgatcgc tttgaagcga ttgcggtacg
     7141 tgtgccaacc ataaatgtga cggcaatcga tttaagcgtg acggtgaaga aacctgtaaa
     7201 agccaatgaa gtcaacctgt ttctgcaaaa agcagcacaa ggtgcatttc atggtatagt
     7261 tgactatacg gaattgccgt tggtctctgt agattttaac cacgatccgc acagtgccat
     7321 tgtcgatggc acccaaaccc gggtcagtgg cgcacacctg atcaaaacgt tggtctggtg
     7381 cgataacgaa tggggctttg ctaaccgaat gctcgacacg acgttagcta tggctactgt
     7441 tgctttcagg taagacgcaa gcagcgtctg caaaactttt agaatcaacg agaggattca
     7501 ccatgtctgt aattaagatg accgatctgg atcttgctgg gaaacgtgta tttatccgtg
     7561 cggatctgaa cgtaccagta aaagacggga aagtaaccag cgacgcgcgt atccgtgctt
     7621 ctctgccgac catcgaactg gccctgaaac aaggcgcaaa agtgatggta acttcccacc
     7681 tgggtcgtcc taccgaaggc gagtacaacg aagaattctc tctgctgccg gttgttaact
     7741 acctgaaaga caaactgtct aacccggttc gtctggttaa agattacctc gacggcgttg
     7801 acgttgctga aggtgaactg gttgttctgg aaaacgttcg cttcaacaaa ggcgagaaga
     7861 aagacgacga aaccctgtcc aaaaaatacg ctgcactgtg tgacgtgttc gtaatggacg
     7921 cattcggtac tgctcaccgc gcgcaggctt ctactcacgg tatcggtaaa ttcgctgacg
     7981 ttgcgtgcgc aggcccgctg ctggcagctg aactggacgc gctgggtaaa gcactgaaag
     8041 aacctgctcg cccgatggtg gctatcgttg gtggttctaa agtatctacc aaactgaccg
     8101 ttctggactc cctgtctaaa atcgctgacc agctgattgt tggtggtggt atcgctaaca
     8161 cctttatcgc ggcacaaggc cacgatgtgg gtaaatccct gtacgaagct gacctggttg
     8221 acgaagctaa acgtctgctg accacctgca acatcccggt tccgtctgat gttcgcgtag
     8281 caaccgagtt ctctgaaact gcaccggcta ccctgaaatc tgttaacgat gtgaaagctg
     8341 acgagcagat cctggatatc ggtgatgctt ccgctcagga actggctgaa atcctgaaga
     8401 atgcgaaaac cattctgtgg aacggtccgg ttggcgtgtt cgaattcccg aacttccgca
     8461 aaggtactga aatcgtggct aacgctatcg cagacagcga agcgttctcc atcgctggcg
     8521 gcggcgacac tctggcagca atcgacctgt tcggcattgc tgacaaaatc tcctacatct
     8581 ccactggcgg cggcgcattc ctcgaattcg tggaaggtaa agtactgcct gcagtagcga
     8641 tgctcgaaga gcgcgctaag aagtaaaaaa tcacagggca gggaaacctg cacttgtttc
     8701 agcgcgcttt tagagcacgc attttttcat actctaaata attcgagttg caggaaggcg
     8761 acaagagagt gaatccccag gagcttacat aagtaagtga ctggggtgag cgaacgcaga
     8821 cgcagcacat gcaacttgaa gtattatgag tataaggccc gacgatacag gacaagagac
     8881 atgtctaaga tttttgattt cgtaaaacct ggcgtaatca ctggtgatga cgtacagaaa
     8941 gttttccagg tagcaaaaga aaacaacttc gcactgccag cagtaaactg cgtcggtact
     9001 gactccatca acgccgtact ggaaactgct gctaaagtta aagcgccggt tatcgttcag
     9061 ttctccaacg gtggtgcttc ctttatcgct ggtaaaggcg tgaaatctga cgttccgcag
     9121 ggtgctgcta tcctgggcgc gatctctggt gcgcatcacg ttcaccagat ggctgaacat
     9181 tatggtgttc cggttatcct gcacactgac cactgcgcga agaaactgct gccgtggatc
     9241 gacggtctgc tggacgcggg tgaaaaacac ttcgcagcta ccggtaagcc gctattctct
     9301 tctcacatga tcgacctgtc tgaagaatct ctgcaagaga acatcgaaat ctgctctaaa
     9361 tacctggagc gtatgtccaa aatcggcatg actctggaaa tcgaactggg ttgcaccggt
     9421 ggtgaagaag acggcgtgga caacagccac atggacgctt ctgcactgta cacccagccg
     9481 gaagacgttg attacgcata caccgagctg agcaaaatca gcccgcgttt caccatcgct
     9541 gcgtccttcg gtaacgtaca cggtgtttac aagccgggta acgtggttct gactccgacc
     9601 atcctgcgtg attctcagga atatgtttct aagaaacaca acctgccgca caacagcctg
     9661 aacttcgtat tccacggtgg ttccggttct actgctcagg aaatcaaaga ctccgtaagc
     9721 tacggcgtag tgaaaatgaa catcgatacc gatacccaat gggcaacctg ggaaggcgtt
     9781 ctgaactact acaaagcgaa cgaagcttat ctgcagggtc agctgggtaa cccgaaaggc
     9841 gaagatcagc cgaacaagaa atactacgat ccgcgcgtat ggctgcgtgc cggtcagact
     9901 tcgatgatcg ctcgtctgga aaaagcattc caggaactga acgcgatcga cgttctgtaa
     9961 gtcattcctt tctgcttatc tcaaggcccg ctctgcgggt ctttttttcg ccaaaagcaa
    10021 ggtgattcag atgagaaatc tgtgatctat ttggcaaaat tatgctttat tgtttaccct
    10081 tgtcagactg cccgtcataa ggcggcggag tgtatttctc cattttgagt cagttgaaaa
    10141 ggaatattga atggaagatt tgaatgttgt cgatagcata aacggcgcgg gaagctggct
    10201 ggtagctaac caggcgctgc tgctaagtta tgcagtaaac atcgtggcgg cactcgcgat
    10261 catcatcgtt ggtttgatta tcgcgcggat gatttccaac gcggtgaatc gcctgatgat
    10321 ctcccgtaaa atcgatgcca ctgttgctga ttttctttct gcattagtcc gttacggtat
    10381 tatcgccttt acgctaatcg ctgccctggg acgcgtgggt gtacaaacag cgtcagtcat
    10441 tgctgtactc ggtgccgcag gcttagctgt tggtctggct ttgcaggggt cactttctaa
    10501 cctggccgct ggcgtgttac ttgtcatgtt ccgcccgttc cgtgccggag aatatgttga
    10561 cctcggcggc gtagccggta ctgtgctgag tgtgcagatt ttctccacca ccatgcgtac
    10621 tgcagacggt aaaattatcg ttattccgaa cggtaaaatt attgccggaa atattattaa
    10681 cttctcccgc gagccagttc gccgtaacga atttattatt ggcgtggcgt atgattccga
    10741 tatcgatcag gttaagcaga tcctgaccaa tattatccag tctgaagatc gcattttgaa
    10801 agatcgcgaa atgactgtgc gcctgaacga acttggtgca tcgtcgatta atttcgtggt
    10861 ccgcgtctgg agcaacagcg gcgatctgca aaacgtgtac tgggatgtgc tggagcgtat
    10921 taaacgtgaa tttgatgccg ccggtatcag cttcccgtac ccgcaaatgg atgtgaactt
    10981 taagcgggtg aaagaagaca aagctgcgta atcaacgctg aggccagata atactctctg
    11041 gcctctctct tattagttat tctgattgcc aattaatatt atcaatttcc gctaataaca
    11101 atcccgcgat atagtctctg catcagatac ttaattcgga atatccaacg tgttttctta
    11161 ttactttcaa ggtcttgctc ttggggcagc tatgattctg ccgctcggcc cacaaaatgc
    11221 ttttgtgatg aatcagggca ttcgtcgtca gtaccacatt atgattgcct tactttgcgc
    11281 tatcagcgat ttggtcctga tttgcgcggg gatttttggt ggtagcgcgt tattgatgca
    11341 gtcgccgtgg ttgctagcgc tggtcacctg gggcggcgta gtcttcttgc tctggtatgg
    11401 ttttggcgct tttaaaacag cgatgagtag taatattgag ttagctagtg ccgaagtcct
    11461 gaagcaaggc agatggaaaa ttatcgccac catgttggca gtgacctggc tgaatccgca
    11521 tgtttacctg gatacttttg ttgtactggg cagccttggc gggcagcttg acgtcgaacc
    11581 aaaacgttgg tttgcgctcg ggacaattag cgcctctttc ctgtggttct ttggtctggc
    11641 tattctcgca gcctggctgg caccgcgcct gcgcacggca aaatcacagc gcattatcaa
    11701 tctggtagtg ggatgtgtca tgtggtttat agccttgcag ctggcgagag acggtattgc
    11761 tcatgcacaa gccttgttca gttaggcgct atctgatgga aaaataaaac agaggcgcta
    11821 agcttgcctc cagaggtcct gaattttggg caagtcaatt aacacggaga gactaacgtg
    11881 aagttcaaag ttatcgccct ggcggcatta atgggtatta gcgggatggc agcgcaggct
    11941 aacgaattgc cggatggacc gcatattgtc acctccggta cggcaagtgt ggatgcggtg
    12001 ccagacattg ccactcttgc gattgaagtt aacgtggccg cgaaggatgc cgctacagcc
    12061 aagaaacagg cagatgagcg cgtcgcacaa tacatttcct tccttgaact caatcagatc
    12121 gcgaaaaaag atatcagctc agcgaattta cgcacccagc cagattatga ctatcaggat
    12181 ggtaaaagta tcctgaaagg ctaccgagct gtgagaacgg tggaagtcac gctccgtcag
    12241 ttagacaaac tgaattcctt gctggatggc gcgctgaagg cgggtcttaa cgaaattcgt
    12301 tctgtgtcgc tgggcgtggc gcagccggat gcctataaag acaaagcgcg taaggcagcg
    12361 attgataacg cgattcatca ggcgcaggaa ctggcgaacg gctttcatcg taaactgggg
    12421 ccggtatata gcgtgcgcta ccatgtttcc aactatcagc ccagcccaat ggtgcggatg
    12481 atgaaagccg atgccgcgcc ggtgtctgcc caggaaactt acgagcaggc cgctattcag
    12541 tttgatgatc aggtcgatgt ggtcttccag ttagaacctg tggatcaaca acccgctaaa
    12601 acacctgcag cacaataata cttgtagccc gacgtattcg tcgggctaat gcctttaccc
    12661 gatattgcct cctttttctg taattttctg taaaaccatg acgtaatcac ctatctttgc
    12721 tcgcggtttc atcaaggata ggctgactga aacggttaag cggacaagga tgcggggagg
    12781 ggggatggac atttttattt cgaaaaagat gcgcaatttc attttattag cgcaaaccaa
    12841 taatattgcc cgggcggcag aaaaaatcca tatgacggct tcgccatttg gtaaaagcat
    12901 tgccgcgctg gaagaacaaa ttggctatac gctatttacc cgcaaagata ataatatcag
    12961 cctcaataaa gccggtcagg aactgtatca aaaactgttc ccggtttatc aacgactttc
    13021 tgcgattgat aatgaaatcc ataactcagg gcgtcgttca cgagaaattg tgattggcat
    13081 agataatact tatccgacaa ttattttcga tcagttgatt agtctcggag ataagtatga
    13141 aggcgtgacc gcccagccag ttgagttcag cgaaaacggc gttatcgata atctttttga
    13201 ccgtcagtta gattttatta tctccccaca gcatgtatct gcacgagttc aggaactgga
    13261 aaacctgact atcagtgaat taccaccgct gcgattgggc tttctcgttt ctcgccgtta
    13321 tgaagagcgt caagagcaag agctgcttca ggagctgccc tggctacaaa tgcgctttca
    13381 aaaccgggct aacttcgaag caatgattga tgcgaatatg cgaccttgtg ggatcaatcc
    13441 caccattatt tatcgtccct acagttttat ggccaaaatc agcgccgtgg agcgcggaca
    13501 ttttctgacg gtgatcccgc attttgcctg gcgactggtg aatccggcaa cgctgaaata
    13561 ttttgatgcg ccgcacaggc cgatgtatat gcaggaatat ctttattcaa tcagaaatca
    13621 tcgttatacc gccacgatct tcagcatatt gctgaagatc gtgacgggac aaaccattaa
    13681 cccagcatcg agccggttgc aattaaatta cggtgtaagt cgaagacgtg gctaagatcg
    13741 tggtgaatat gtccgccggg cgcattttcc agatagcgat gcagataatc ccgatacata
    13801 ggatgtgcac aattatcaat gatagtgcgg gcgcgttgaa gcggggaaag accgcgcaga
    13861 tcggcgatcc cttgttcggt gatgatcact ttgacgctgt gttcgctgtg atcaacatgg
    13921 ctgcacattg gcacgacggt tgagatcttg ccttctttag caatcgacgg agccataaag
    13981 atcgacagat acgcgttgcg ttcaaaatca ccgctgccgc cgatgccgtt catcagatcg
    14041 accccggcta cgtgcgttga atttgcatgc ccgtaaatat caaactccag gccgacgttc
    14101 agagcgatga cgcccagacg acggatgatt tccgggttat tggaaatctc ctgcggacgc
    14161 aacacaatgc ggctggcaaa gtaatccata ttgtcgtaaa tcttgcgcag ggaatcggcc
    14221 gagattgtca ggctggaggc gctggccccg ctgattttgc cggtttccag taaatgcacc
    14281 accgattcct gtagcacttc cgaatacatc atgaacggag gaatttccgg gttttccccc
    14341 agacgtgcca ttaccgcatt attgatattg cccacgccac tttgcagcgg cagaaattcc
    14401 ggcggaatac gcccatgcgc catttcctgc aataagaacg tgaccacgtt atcggcaatc
    14461 tgctggcaca tgggattttg cttatccagc atattaccgg cgtcgggcaa gttggtttcc
    14521 acgacggcga caatcttttt cggatcgatt tgcacatagc gggtaccgac gcgatccatt
    14581 gcatggaaga tcgacacgct attgcgccgc ggtggcgcgc caggaatcac aatatctgcc
    14641 agttctgcaa cgcgcggatc gtgatagtga ttaagttcaa tgatcacttt cttcgctcgc
    14701 agcagccagg tcggcgcatt accgatcccg ctggttaacc agactcgacc atccggtgcc
    14761 agtgccgatg cttcaatgac ggcaacatca atgtcgccga agaaaccgta attgaccatt
    14821 tgcgccactt cgctcaaatg caggtcaacg aaactcaccg cgccctgatt gatcttttta
    14881 cgtaaactgg acgatgtttg atatggcgca cgccaggaaa cagcatcggc gtcagaaagt
    14941 acatcgtcag cggcggcgct gattgacgcc cccgtcagca ggcgaatttg atacggcttt
    15001 ttggcctcat gctgttcgtt agctctgcgg gcaatcgcgg cgggtagggc tttcggcgaa
    15061 cccgctgggg taaagccgct aaatgccacc atgtcgttat gctggataat ttctgccgct
    15121 tcatcggcgg tcatccttgt ccactgagtt tccattttta ctccttcgtt cgcagggatt
    15181 aatgaccaac gaaattaggt ttacgttttt cgaggaaagc gttcatccct tcctggtaat
    15241 cttcgctgtc ataaaccgcg cggcgcatcc cctgaatacg ttcaaattca tcggagttca
    15301 tggtgtgtgc ttcgcccagt acacgcagct cttctttgat aacggcaatg gctaacggcg
    15361 ctttctcaga gatgtggtgc gccatttgta aggtgaaatc ttccagttct tccacttcca
    15421 caacatggtt gaggatgccg acagccagcg cgcgctgggc ggtgattggc gaagcggtaa
    15481 aaatcagctc tttgacaatg tggaagcccg cgtcgcgggt caggttgtga atgccgacca
    15541 ggttatacgg gacgccgagg tttacaggcg tcattgagaa ggttgaggta ctggcggcga
    15601 tgatcagatc ggaactcatg atcatttcaa atgcgccacc ccaaacacta ccttccacca
    15661 tcgaaatgat cggtttcggg aatttttgga tcatgcgggt gatttgacgc aatggatcat
    15721 cataggagag cggatcgcga ccgccagacg gcagttcgtg aatatcgtga cctgcggaga
    15781 agactttgga tccacttggc gcccgcaaaa tgatacagcg aatttccggc cggttgagat
    15841 cgcttaacgc ctgcataaga tcatcaataa agactttact taaggcatta agttttcgtc
    15901 catagttaaa ctcaatgacc gccactttgt tgatagtggc aacgttcaca tactgataag
    15961 acataaaaat tcctttaatc aaaatattgc gtctggataa attcactgag ctgccgcagg
    16021 ccggtgcgcg gtgagagcgt attgtttttg accgctaaaa gcgtctggcg gtaatagcga
    16081 tcgaaatctt cattcgcgaa caggtgattc agtacttctt cttcggtctg cttacgcagc
    16141 cattccaccg attgttgttg ccgcacttgt tgtaaacgac cactggcagt tagcgcggtt
    16201 ttgaagtcga tgatggcgtg ccagatctca tcgattccac gtttttccag tgcgctacaa
    16261 gtcagacccc gtggctgcca ttcgtcgtat ttacgtcgca gaatatgcag ggcactctcg
    16321 tacatatgcc gggcaatggc gacattggta tggttatcgc catcgtcttt gttgataacg
    16381 agcagatcag ccacttccat cagccctttt ttaatgccct gcagatcatc gccgccaccg
    16441 gcaatttgca acgagataaa acagtccacc atgcgggcga cttccgtttc cgactgcgcg
    16501 acgccaaccg tttcgacaat cactacgtca taacccgccg cttcgcataa cagcattaat
    16561 tcccgtgctc gctgactggc accgcccaga tgaccggagg agggtaccgg gcgaataaac
    16621 gccgcctcgg cacgcgccag gtcattcatg cgggttttat ccccgagaat gctaccgcca
    16681 gtgaccgggc tgctgggatc gaccgcaata acagcaacct ttaatccctc acgaatcaac
    16741 aacatgccaa aggcttcaag aaaggtactt ttccccgcgc cgggtgtgcc ggtaacgccc
    16801 agtcgcaggg cgttaccaca gaacggcata atagcgtcaa gcagctgtgt acttagcgcc
    16861 tgatgacgtg ggtgacggct ttccaccagc gtcatggctt gggcgagcgt ggcacgctca
    16921 ccctgacgta agcggcgaat actttctgcc agcgtggctt cattaatcat gatgctggct
    16981 tatcagattc agtacgtcgc gcacactgtc gagcataggt gtacctggac cataaatcgc
    17041 cgccacgccg cgctcttgca ggaaggcgta atcctgcggc ggaatgacgc cacccgcgac
    17101 cacgcagata tcttcgcgtc cccatttttt cagcgcttcg accagttccg ggatcagcgt
    17161 tttatgaccg gcagccagtg aggatgcgcc cactacgtga acgtcgtttt ctacggccag
    17221 gcgggcgatc tcttcaggtg tagagaacat cgggcttaaa tctacgtcga aaccgagatc
    17281 ggaataggcg ctggcgatca ctttcgcgcc gcgatcgtgt ccatcctggc ccatcttagc
    17341 gatcagaatg cgcgggcgac gaccattgtc ggcaaggaac tgctccgttt gcgcaacaat
    17401 ggcatcgaac tcggaggccg atttctcaga ctgatgatag ctttgcgcaa tcacgccggt
    17461 aacacactgg cttggcacca gataacggtc gaaagcgact tccagcgcat cggaaatttc
    17521 acccagggtg gcgcgaacgc gagcggcatt aacagcggca gccagcaggt tttcgttatg
    17581 ctgtgcggcg tgagtcaggg cgttcaacgc ggcggttacg gcggcatcat cacgggtggc
    17641 gcgaatgcgt tccagcgaag caatttgctc gttacgcacc atcacgttgt cgatctcaag
    17701 tacatcggtt tcgtcttcgt gatccagttt gtacttgttg acaccaacga tgacacgctt
    17761 gccctggtcg atcagcgact gttcgcgcgc tgaggcctct tcgatcattc gttttggcag
    17821 acctgcttcg atcgctttcg ccatgccacc ggcttcgtcg atctgttgga taatagctct
    17881 ggcttgtttg acgatttgat cggtcagcga ctcaatgtaa taggatccgg ccagtggatc
    17941 gacggtgtgg cagagttctg attcttcctg gatgatgatc tgggtgttgc gggcaatgcg
    18001 tgctgagaaa tcggtaggca aaccaagcgc ttcgtcaaag gcgttggtat gcagtgactg
    18061 agtaccgccc agcgtcgcag ccagtgcttc aatggtggtg cggataacgt tgttatacgg
    18121 atcctgttca gtcaggctcc agcctgaggt ctggcagtgg gtacgcagcg ccagtgattt
    18181 cggatcctgt gcgccaaatc cactgaccgc ttcgctccat aaataacgtg ccgcacgcaa
    18241 catggcgacg ttcataaaca gatccatgcc gataccgaag aataacgaca ggcgaggagc
    18301 gaagtcatca attttcagtc cggcagagat tgctgctttg atgtactcaa tcccatcggc
    18361 gagcgtaaat gctacctgct gcacgcagtt ggcacccgct tcccccatgt ggtaaccgct
    18421 gatactgatg gtattaaatc gcggcatgtt gccggaacac caggcgatga tgtcggcgat
    18481 aatgcgcatt gacggttttg gtgggtaaat ataggtgttg cggcagaggt actctttgag
    18541 aatatcgttt tgaatggtgc cggtcagttt atcaggtgta acaccttgct cttctgcggc
    18601 gacgatataa aacgccagta ctggtagcac tgcgccattc atggtcatcg aaaccgacat
    18661 tttatccagc gggatctggt cgaacaggac tttcatatct tccacggtgt cgatagcgac
    18721 gcccgctttg ccgacgtcac ccgccacgcg tgggttatcg gagtcgtagc cacggtgggt
    18781 ggcaaggtca aacgcaacgg aaagaccttt ttgcccggcg gccaggttac ggcgataaaa
    18841 agcgttggac tcttttgctg ttgaaaaacc agcatactga cggatggtcc acggttgggc
    18901 ggtatacata gtggcacgcg ggccacgaac gtagggcggc aaaccaggaa gggtacctgt
    18961 cacctccaga ttatcgagat cggcttcggt atacagcggc ttgatggcga tcccttccgc
    19021 ggtttgctga accagcgagt cgacagtttt ctcccgacgg ctcaattcct tgttggcaag
    19081 ctgttgccac tcctgctcgt tagacattat ttgctccgta aaacgcaatc aaaatgaggg
    19141 tatttgcgct tacggtgagc tttttacgtg ggtttgtcgc cagcaaaaaa aatgcattca
    19201 gacgtcgttt tttgccgcct gagatttgtc tattttgtga tgtgattcaa ggaatacaaa
    19261 aagaagtgag gacaacgcct gatatgtgct atcaggcatt atttgatgga ttaatcctga
    19321 cgaaggactt tgtgaccata atcgagtaac gcatcagtga ctttacgcat catgcggctt
    19381 tcaggagcaa agcggtgcca gtagagcatc cgtcgttgaa atagcccagg cgttaagtca
    19441 atcagttcac cactggccag ctctttctcg atttgcaggt gcgggatcat acagcaggtg
    19501 gtgccctggc gagcaagttg tacgaacgct tctgaagaat taacgatatg gcagggtacg
    19561 ctgccaggag gaagatcgaa gttttgctgc aaaaaggcct ggtgcatatc gtcaagatgg
    19621 tcaaacgcga ccactggcgc tttcaataat gccgaacgcg ttacgccgtt aggaaaatat
    19681 ttttcggcaa acggttttga gctgacgaac agatagtcga gcgcaccgag tttatcgaca
    19741 agacaactcg gcagcgcctg atgttgaata ctcaccgcgc cgaccacttc gccgcggcgc
    19801 agacgttcct gagtacgggt ttcatcttct acctgcaagt tgaggcggat aggcgaatca
    19861 gccaacacag gagccagtgc cggaagcaac cacgtcgcca gactgtcggc gttgaccgcc
    19921 agtgaaagca gcagcggagt cgaaccggtt tgttcatcgc ccagccactc ttcttccagc
    19981 aactccacct ggcgcagcag tgccagcagt ttttgccctt gttccgtcgg gcgcggcggt
    20041 acggtacgca ccaacagcgg ctgcccgaac atattttcca gttgcttaat gcgctgtgag
    20101 acggctgatt gtgtaatgca cagcttttgt gccgcgcgct caaatcctcg ttcacgtatc
    20161 accgcatcca gtgcctgtaa tgttctgtag tccgggcgtt tcattgctgt tgtctgctcc
    20221 tggtaaatgc tttttcctgc actatgacac aattttgtgt caggttgcat ataccgaagc
    20281 cgtcggcggg taacatttgt atcaatggtt aaacgcgcag cttaaaagat cagggatttg
    20341 cgctattgca tttcgcttag cgacttcgcg ccctggttat gttgaatggc gtggcgttat
    20401 tgcctctgtt cgcaccgtgg agcggcatac ccagaccgtt gtatgcttta tttgcggggc
    20461 gaaaggggat gcccgccatt gcgcgatatt ttatggatga gttaaccacc cggcttgcca
    20521 acggggtctg aatcgctttt tttgtatata atgcgtgtga aatttcatac cacaggcgaa
    20581 acgatcatga cgcaggatga attgaaaaaa gcagtaggat gggcggcact tcagtatgtt
    20641 cagcccggta ccattgttgg tgtaggtaca ggttccaccg ccgcacactt tattgacgcg
    20701 ctcggcacga tgaaaggcca gattgaaggg gccgtttcca gttcagatgc ttccactgaa
    20761 aaactgaaaa gcctcggcat tcacgttttt gatctcaacg aagtcgacag ccttggcatc
    20821 tacgttgatg gcgcagatga aatcaacggc cacatgcaaa tgatcaaagg cggcggcgcg
    20881 gctctgaccc gtgaaaaaat cattgcttcg gttgcggaaa aatttatctg tattgccgac
    20941 gcctccaagc aggttgatat tctgggtaaa ttcccgctgc cagtagaagt tatcccgatg
    21001 gcacgtagtg cagtggcgcg ccagctggtg aaactgggcg gtcgtccgga ataccgtcag
    21061 ggcgtggtga ccgataatgg caacgtgatc ctcgacgtcc acggcatgga aatccttgac
    21121 ccgatagcga tggaaaacgc cataaatgcg attcctggcg tggtgactgt tggcttgttt
    21181 gctaaccgtg gcgcggacgt tgcgctgatt ggcacacctg acggtgtcaa aaccattgtg
    21241 aaatgatctg acgggggaac ctcccccgtt aaaaaaattc tcttcattaa atttggtgac
    21301 atgtgtcacg cttttaccag gcaattgtcg attgctctaa ataaatcctc taaaccagca
    21361 tattcatcca agaattacct ttgcgtgata tttcctcaac atcgcgacgc aaacgttcat
    21421 attgccgcaa tattattttt tgatatgttg aaaggcggat gcaaatccgc acacaacatt
    21481 tcaaaagaca ggattgggta aatggcaaag gtatcgctgg agaaagacaa gattaagttt
    21541 ctgctggtag aaggcgtgca ccaaaaggcg ctggaaagcc ttcgtgcagc tggttacacc
    21601 aacatcgaat ttcacaaagg cgcgctggat gatgaacaat taaaagaatc catccgcgat
    21661 gcccacttca tcggcctgcg atcccgtacc catctgactg aagacgtgat caacgccgca
    21721 gaaaaactgg tcgctattgg ctgtttctgt atcggaacaa accaggttga tctggatgcg
    21781 gcggcaaagc gcgggatccc ggtatttaac gcaccgttct caaatacgcg ctctgttgcg
    21841 gagctggtga ttggcgaact gctgctgcta ttgcgcggcg tgccggaagc caatgctaaa
    21901 gcgcaccgtg gcgtgtggaa caaactggcg gcgggttctt ttgaagcgcg cggcaaaaag
    21961 ctgggtatca tcggctacgg tcatattggt acgcaattgg gcattctggc tgaatcgctg
    22021 ggaatgtatg tttactttta tgatattgaa aacaaactgc cgctgggcaa cgccactcag
    22081 gtacagcatc tttctgacct gctgaatatg agcgatgtgg tgagtctgca tgtaccagag
    22141 aatccgtcca ccaaaaatat gatgggcgcg aaagagattt cgctaatgaa gcccggctcg
    22201 ctgctgatta atgcctcgcg cggtactgtg gtggatattc cggcgctgtg tgatgcgctg
    22261 gcgagcaaac atctggcggg ggcggcaatc gacgtattcc cgacggaacc ggcgaccaat
    22321 agcgatccat ttacctctcc actgtgtgaa ttcgacaacg tccttctgac gccacacatt
    22381 ggcggttcga ctcaggaagc gcaggagaat atcggcttgg aagttgcggg taaattgatc
    22441 aagtattctg acaatggctc aacgctctct gcggtgaact tcccggaagt ctcgctgcca
    22501 ctgcatggtg gtcgtcgtct gatgcacatc cacgaaaacc gtccgggcgt gctaactgcg
    22561 ctgaacaaaa tcttcgccga gcagggcgtc aacatcgccg cgcaatatct acaaacttcc
    22621 gcccagatgg gttatgtagt tattgatatt gaagctgacg aagacgttgc cgaaaaagcg
    22681 ctgcaggcaa tgaaagctat tccgggtacc attcgcgccc gtctgctgta ctaattcccc
    22741 ttctctgaaa attaacgggc aggtcactga cttgcccgtt tttttatccc ttctccacac
    22801 cgttgccgcg ttgcgcattc tactttgcga gtctgctcca gaaaataaat caaagtcaga
    22861 tcaatgcgtt gtgtttattt aatggggatt tacgatggcg ggacagcatg gggctgtaac
    22921 ggtaaagccc tcaccgaagc gagggcttaa aggagaaggg ttatgatgcg acttgtcatc
    22981 atactgattg tactgttact gataagtttc ggagcttatt aacagtcagt ctcaagggag
    23041 gagtcatcct cccttaccct tactcactaa attaggtcaa agaatcaacg atgtcaatca
    23101 gggcgatgcg gggtgcatcg ccctgaaccg aactttttga tagccacttc atcgcccacg
    23161 tttactgccg taatagatcc tttttcccaa aaattatctc tgttttgcat ggggtttttc
    23221 gagcacgctc gaagaaaaga tttaaaggta gatcaatgcg ttgtggttat ttaaagagag
    23281 cttacgatag ctgggcagca tggggctgta acggtaaagc cctcaccgaa gcgagggctt
    23341 gaaggagaag ggttatgatg cgacttgcca tcatactgat tgtactgtta ctcataagtt
    23401 tcccagctta ttaacagtca gtcacagggg aggagcaatc ctcccttacc cttactcact
    23461 aaattaggtt aaagaatcaa cgatgtcaat cagggcgatg caggtgtatc gcccttacca
    23521 ctcccagact ttcgacggtg taaccaccgc aggaagaggg atatcccact cttcaacggg
    23581 gagtttttcc accaactgac aatcatgcgc ataacccacc ggttgcgttt tatagtgctg
    23641 ccagttttgt aaggtccgat cataaaaacc accgcccatt cccaggcgct gaccgtactc
    23701 atcaaaggcg accagcggtg tgatcagcac gtctaatcgg gaaaggggta gcacgtcacg
    23761 cacatccagt tttggctcat ggatcttcaa cctgttcatc accagttcgc tttgcggatg
    23821 gtaattcagg aacagcaaat taccggcact aaagggatgc aaaactggaa gatatacgcg
    23881 cttactggcg cgccagagtt gttctattag cggctgggtg tcgagttcac catcaaaaga
    23941 gaggaataca gcgaccgtat gcgccatcac caccggggga taagtcatca tccgggtagc
    24001 ggcttgttga cccatttcca gctgttgttc cggcgttaac gcacgacgac gttgccgaat
    24061 cattttgcgg atttcttgtc gggataatgt cagtgggagc tctggtagtt gcgtcatggc
    24121 tggtgccaga taagaaggga atctccgaga tgccgccgca ggctgtaacc cttgaaccct
    24181 tggttcaagg tgaatgtgtc gtcgcagttt taaggcttct cggacgaacc gagcatgctc
    24241 accaaccgcg gagcgccaca ttcttgtggt atgaaatatc ggctcagggg actggcccgc
    24301 ttgcgaacat ctcagagaaa ttttgtcttc acggttactc taccacagta aaccgaaaag
    24361 tgtcattcaa agttttggtt agttttttcg gtgatgcgac cttgttcaag taacgcttgt
    24421 tctatggtct gctgcagcat ccgaatacgc tgttccatac ttgccgcgta gtcacgagtc
    24481 tttgcttttt cttgcgctaa ctcatagctg atattcaatg cggcaatgaa gaccaactgt
    24541 tcagtatttg tgactctagt gcgttctttc agatcttgca accgttggtt cagatcgtcc
    24601 gctgcctgat tcaacgcatc cctttggtca ggcgggcagt tcacacgcag tgaacggcca
    24661 aaaatttgga tatcgacggg ttgtgcagac atgccacctt cctgctgatt gactgcgctg
    24721 ccttcgtctt tagacccatg gtctgcgaag gggcgacact atagctaccc tgatgagaag
    24781 agacaagccc ttttctggtc caccagggcc caaagtggta gcatatcatg aatattcctc
    24841 cctttgacga cgaatgctta tgtctataca gaacgaaatg cctggttaca acgaaatgaa
    24901 ccagtatctg aaccaacaag ggacgggtct gacccctgcc gagatgcatg gtttaatcag
    24961 tgggatgata tgtggcggta acgatgacag ctcatggctg ccgctacttc acgacctgac
    25021 gaacgaaggc atggctttcg gtcatgagct ggcacaggca ctgcgtaaaa tgcactctgc
    25081 caccagcgat gccctgcagg atgacggctt cctttttcag ctttatctgc ctgatggcga
    25141 tgatgtcagc gttttcgatc gggctgatgc actggcaggt tgggtcaatc acttcctgct
    25201 tggtcttggc gttacgcaac cgaagctgga caaagtgacc ggcgaaaccg gtgaagccat
    25261 cgacgatctg cgtaacatcg cgcagctggg ctacgacgaa gacgaagatc aggaagagct
    25321 tgaaatgtcg cttgaagaga tcatcgaata cgttcgtgtt gccgcgctgt tatgccacga
    25381 cacctttact catccgcaac cgaccgcgcc agaagtacaa aaaccgactc tacactaaaa
    25441 acaaaaaacg taaggagagt gttatgagtg agatatcccg gcaagagttt cagcgtcgcc
    25501 gtcaggccct ggtggagcaa atgcaacccg gcagcgccgc gctgattttt gctgcaccag
    25561 aagtaacacg tagcgccgac agcgaatacc cctatcgtca gaacagtgac ttctggtact
    25621 tcaccggctt taacgaaccg gaagcggtgc tggtgctgat taaaagcgat gacactcata
    25681 accacagcgt tctgtttaac cgcgttcgcg acctgacggc ggagatctgg tttggccgtc
    25741 gcttaggcca ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg gcattcagcg
    25801 aaatcaatca gcaactttat caactactta acggcctgga tgtggtttac catgcccagg
    25861 gcgaatatgc atatgctgat gaaatcgtga acagtgcgct ggaaaaactg cgtaaaggtt
    25921 cgcggcaaaa tctcaccgca ccggcaacga tgatcgactg gcgtcctgtt gttcatgaaa
    25981 tgcgcctgtt caaatcgcca gaagagattg ccgtactccg ccgcgcggga gaaatcaccg
    26041 ccatggcaca tacacgggcg atggaaaaat gccgtccggg aatgttcgag taccatctgg
    26101 aaggcgaaat tcaccacgaa tttaaccgcc acggtgcgcg ctatccttcc tataacacca
    26161 ttgtaggcag cggtgaaaac ggctgcattc tgcactacac cgaaaacgag agtgaactgc
    26221 gcgacggtga cctggtgttg attgacgcgg gttgtgaata caaaggttac gcgggcgata
    26281 ttacccgcac cttcccggtc aacggcaaat tcacccaggc ccagcgtgaa atctacgaca
    26341 ttgtgctgga gtctctcgaa accagcctgc gcctgtatcg tccgggaact tccattctgg
    26401 aagtcactgg tgaagtggtg cgcatcatgg ttagcggcct ggtaaaactc ggcatcctga
    26461 aaggtaatgt tgatgaactg atcgctcaga acgcccatcg tcctttcttt atgcatggcc
    26521 ttagccactg gttaggactg gatgtccatg acgttggcgt ttatggtcag gatcgctcgc
    26581 gcattctgga accggggatg gtactgaccg tagagcctgg tctgtatatc gcgccggatg
    26641 cggatgtgcc agaacaatat cgcggtatcg gcattcgcat tgaagacgac attgtgatta
    26701 ccgaaaccgg caacgaaaac ctcaccgcca gcgtggtgaa aaagccggaa gaaatcgaag
    26761 cgttgatggc tgctgcgaga aagcaatgag cgtaatcatc gtcggtggcg gcatggcggg
    26821 cgcgacgctg gcgctggcta tttcccggtt aagtcacggg gcgctgccgg tacatttgat
    26881 tgaagcgact gcgccagagt cacatgctca tccgggcttt gatggacgag cgatagcgct
    26941 ggcggcgggt acctgtcagc aactggcgcg catcggcgtc tggcaatctc tggctgattg
    27001 cgcaaccgcc atcactaccg tgcatgtcag cgatcgtggt catgctggat tcgtcaccct
    27061 cgccgcagaa gattaccaac tggcggcgct ggggcaggtt gtcgaattgc ataatgtcgg
    27121 gcaacggctg tttgcattgc tgcgaaaagc acctggcgta atgctgcatt gccccgatcg
    27181 cgtagctaac gttgcccgta ctcagagtca tgttgaagtg acgctggaga gcggcgagat
    27241 gctgacgggc cgcgtgctga tagccgctga tggcacccat tcagcgttag ccaccgcgtg
    27301 cggcgttgat tggcagcagg agccttacga acaactggca gtgattgcca acgttgctac
    27361 ttctgttgcg cacgaaggcc gcgcttttga acgcttcacg caacatggcc cgctggcgat
    27421 gttgccgatg tctgacggac gctgttcgct ggtctggtgt catccactgg aacggcgcga
    27481 agaggtgctg tcgtggagcg acgagaagtt ttgccgtgaa ctccagtctg cctttggctg
    27541 gcgactgggg caaattaccc acgccggtaa acgcagtgct tatccgctgg cattaacccg
    27601 cgccgccaga ccgattaccc atcgcactgt gctggtgggc aatgcggcgc aaactctgca
    27661 cccgattgcc gggcaagggt ttaacctcgg tatgcgagat gtcatgagcc ttgcggaaac
    27721 cctgactcag gcgcatgagc gcggagaaga catcggtgat tacggcatat tgtgccgcta
    27781 tcagcagcgt cgacagagcg atcgcgaagc aaccatcggc gtcacggaca gcctggtaca
    27841 tctttttgcc aaccgttggg caccgctggt tgtcgggcgc aacatcggac tgatgacgat
    27901 ggaattattc accccggcac gcgatgtgct ggcgcagcgc accctcggtt gggtggcgcg
    27961 ttgaaggcgg ttttaaggaa taaacaatgc aaagtgttga tgtagccatt gttggcggcg
    28021 gcatggtggg gctggcggtt gcctgcggct tacaggggag cggcttacgc gttgccgtac
    28081 tggagcagcg cgtaccggaa cctctggcgg tagatgcact accacaactg cgcgtttcgg
    28141 ctatcaatgc cgccagcgaa aaattactca cccgtcttgg cgtctggcag gacattctcg
    28201 ctcgtagggc cagttgttat cacggcatgg aagtgtggga caaagacagt tttggtcaca
    28261 tctcgtttga cgatcaaagc atgggctata gccatcttgg gcacatcgtt gaaaactcag
    28321 tgattcacta cgcgctgtgg aacaaagcgc agcagtcgtc agatattact ctgttggccc
    28381 ccacagaatt acagcaggtc gcctggggag aaaatgaaac cttcctgacg ctgaaagatg
    28441 gcagcatgtt aacggcgcgt ctggtgattg gcgcggacgg tgctaattcc tggttgcgca
    28501 acaaagcaga tattccgctg actttctggg attatcagca tcacgcgctg gtagcgacca
    28561 ttcgcacgga agaaccgcat gatgcggtgg cgcggcaggt tttccatggc gaaggcattc
    28621 tggccttttt accgcttagc gatccgcatc tttgctcgat tgtctggtca ctgtcgccag
    28681 aggaagcgca gcggatgcag caggcaagtg aagacgaatt taatcgcgcg ttaaatatcg
    28741 cttttgataa tcgccttggc ttatgcaagg ttgagagcgc gcgtcaggtg ttcccgctga
    28801 cggggcgtta tgcgcgccag tttgccgcac accgtctggc gctggtgggc gacgccgcgc
    28861 ataccattca cccactggcg gggcagggcg ttaatctcgg atttatggac gccgcagagc
    28921 tgattgctga actgaaacgg ttgcatcgtc agggtaaaga catcgggcag tacatttacc
    28981 tgcgccgcta tgaacgtagc cgcaagcaca gcgcggcgct gatgctggct ggtatgcagg
    29041 gttttcgcga tctcttttcc ggcgctaatc cggcgaaaaa actgctgcgt gatattggtt
    29101 tgaaactggc cgacacgctt cctggcgtta agccgcaact tatccgccag gcaatgggat
    29161 taaacgattt gcctgaatgg ctgcgttaaa aatttctcct ctgttgttta tttgataccc
    29221 atcacacttt catctcccgg ttttttcgcc gggagatttt cctcatttga aataaactaa
    29281 tttcacctcc gttttcgcat tatattttct aataccatga ttttttgatt tagtgttttt
    29341 tgacattttt ttagctctta atattgtctt attcaaattg actttctcat cacatcatct
    29401 ttgtatagaa attggtgtat tttttggttt tttattctgt cgcgattttt gcatttttta
    29461 accataagct aatgtgatga tcaattttac cttatggtta acagtctgtt tcggtggtaa
    29521 gttcaggcaa aagagaacga ttgcgttggg gaccgggagt ggctccgatg ctgggttttg
    29581 tggtgataat ttcaccatga aaaagttgtc agccccgctt attcaatgag gacaagatgg
    29641 cacaacagac tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc
    29701 acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta
    29761 ccgatgccgg aatgtttgat gtgtcgcata tgaccatcgt cgatctccgc ggcagccgca
    29821 cctgggagtt tctgcgttat ctgctggcga acgatgtggc gaagctcacc aaaagcggca
    29881 aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgat ctcatcgtct
    29941 actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc
    30001 tctcctggat tacccaacac gctgaacctt tcggcatcga aattaccgtt cgtgatgacc
    30061 tttccatgat tgccgtgcaa gggccgaatg ctcaggcaaa agctgccaca ctgtttaatg
    30121 atgctcagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gtgggcgatc
    30181 tgtttattgc caccactggt tataccggtg aagcgggcta tgaaattgcg ctgcccaatg
    30241 aaaaagcggc cgatttctgg cgtgcgctgg tggaagcggg cgttaagccc tgcggtctgg
    30301 gggcgcgtga cacgctgcgt ctggaagcgg gcatgaatct ttatggtcag gagatggacg
    30361 aaactatttc tcctttagcc gccaacatgg gctggactat cgcctgggaa ccgacagatc
    30421 gtgactttat cggtcgtgaa gccctggaag tgcagcgtga gcatggtaca gaaaaactgg
    30481 ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgctttaccg
    30541 atgcgcaggg caaccagcat gaaggcatta tcaccagcgg tactttctcc ccgacgctgg
    30601 gttacagcat tgcgctggcg cgcgtgccgg aaggtattgg cgaaacggcg attgtgcaaa
    30661 ttcgcaaccg tgaaatgccg gttaaagtga caaaacctgt ttttgtgcgt aacggcaaag
    30721 ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag cagaactgaa
    30781 atacagcaaa gaacacgaat ggctgcgtaa agaagccgac ggcacttaca ccgttggtat
    30841 taccgaacat gctcaggagc tgttaggcga tatggtgttt gttgacctgc cggaagtggg
    30901 cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa tcggtaaaag cggcgtcaga
    30961 catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gacgcactga gcgattcccc
    31021 ggaactggtg aacagcgaac cgtatgcagg cggctggatc tttaaaatca aagccagcga
    31081 tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac gaagcattgt tagaagatga
    31141 gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggaaaatt tatgcctcac
    31201 cctcactctc ttcgtaagga gagaggttca caattcactg cacgtttcag gaaccatcgc
    31261 tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg
    31321 gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggtgcacaa tcgttaaacg
    31381 cgctgaccgg ccagattgtg ccgaaagata ttcagcttgc gacaccaccg caggttggcg
    31441 caccggcgac cgaatacgcc gcactggcag aactcaaggc tattgccagt cgcaataaac
    31501 gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc
    31561 gtaacatgct ggaaaatccg ggctggtata ccgcgtacac tccgtatcag cctgaagtct
    31621 cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat ttgactggac
    31681 tggatatggc ctctgcttct cttctggacg aggccaccgc tgccgccgaa gcaatggcga
    31741 tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct tccaatgtgc
    31801 atccgcaaac gctggatgtg gtccgtactc gtgctgaaac ctttggtttt gaagtgattg
    31861 tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg
    31921 taggcactac cggtgaaatt cacgactaca ctgcgcttat tagcgaactg aaatcacgca
    31981 aaattgtggt cagcgttgcc gccgatatta tggcgctggt gctgttaact gcgccgggta
    32041 aacagggcgc ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg atgggctacg
    32101 gtggcccaca cgcggcattc tttgcggcga aagatgaata caaacgctca atgccgggcc
    32161 gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg gcgatgcaga
    32221 ctcgcgagca acatattcgt cgtgagaaag cgaactccaa catttgtact tcccaggtac
    32281 tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccggttggc ctgaaacgta
    32341 tcgctaaccg cattcaccgt ctgaccgata ttctggcggc gggcctgcaa caaaaaggtc
    32401 tgaaactgcg ccatgcgcac tatttcgaca ccttgtgtgt ggaagtggcc gacaaagcgg
    32461 gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatc ctgaacgccg
    32521 tggggatcac ccttgatgaa accaccacgc gtgaaaacgt aatgcagctt ttcagtgtgc
    32581 tgctgggcga taaccacggg ctggagatcg acacgctgga caaagacgtg gctcacgaca
    32641 gccgctctat ccagcctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta
    32701 atcgctatca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc
    32761 tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg
    32821 ccgagatgat cccaatcact tggacggaat ttgccgaact gcacccgttt tgcccgccgg
    32881 aacaggctga aggttatcag cagatgattg cgcagctggc tgactggctg gtgaaactga
    32941 ccggttacga cgccgtttgt atgcagccga actccggcgc acagggcgaa tacgcgggcc
    33001 tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga
    33061 tcccggcttc tgcgcacgga actaaccccg cttctgcaca tatggcagga atgcaggtgg
    33121 tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg
    33181 aacaggcggg cgataacctc tcctgtatca tggtgactta tccttctacc cacggcgtgt
    33241 atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc
    33301 ttgatggcgc gaacatgaac gcccaggttg gcatcacctc gccgggcttt attggcgcgg
    33361 acgtttcgca cctcaacctg cataaaactt tctgcattcc gcacggcggt ggtggtccgg
    33421 gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg
    33481 tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc tgcggcaccg ttcggtagcg
    33541 cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa
    33601 aagcaagcca ggtggcaatc ctcaacgcca actatattgc cagccgcctg caggatgcct
    33661 tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc
    33721 gcccgctgaa agaagaaacc ggcatcagcg agctggatat tgccaagcgc ctgatcgact
    33781 acggtttcca cgcgccgacg atgtcgttcc cggtggcggg tacgctgatg gttgaaccga
    33841 ctgaatctga aagcaaagtg gaactggatc gctttatcga cgcgatgctg gctatccgcg
    33901 cagaaattga ccaggtgaaa gccggtgtct ggccgctgga agataacccg ctggtgaacg
    33961 cgccgcacat tcagagcgaa ctggtcgccg agtgggcgca tccgtacagc cgtgaagttg
    34021 cggtattccc ggcaggcgtg gcagacaaat actggccgac ggtgaaacgt ctggatgatg
    34081 tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattca
    34141 ctgattcgag tattttctaa aggcgcttcg gcgccttttt agtcagatga caaagtacaa
    34201 aagtgctcag acagtcccct cgcccctttg gggagagggt tagggtgagg ggaacaggcc
    34261 ggcactggcg cgaatattta ccctcacccc gaccctctcc ctgaaagggc gagggggaaa
    34321 agcgtgccaa cattgaagat tgagccagtt tgttagcaat ctcaaagata cgtcaacgaa
    34381 ttaatttttc tcggaaaaac aaatggctat agcacttgtg accggtggca gtcgcggcat
    34441 cgggcgggca actgcattac tgttggcgca agaagggtat acggtggcgg ttaattatca
    34501 gcaaaacctc cacgcggcgc aggaagtgat gaacttaata atgcaagccg gtggcaaagc
    34561 attcgtgctc caggcggata tcagcgacga aaaccaggtc gttgcgatgt ttacagcaat
    34621 cgatcagcac gatgaaccgc tagcagcgct ggtcaataac gccgggatct tgtttaccca
    34681 gtgcaccgtt gaaaacctta ccgcagagcg aatcaaccga gtactttcca ccaacgtgac
    34741 gggatatttt ctctgctgcc gcgaggcggt aaaacgcatg gcgcttaaaa atggtggcag
    34801 tggcggcgct atcgtcaatg tctcttcggt ggcctcacgg ttgggttcgc caggggaata
    34861 tgttgattac gcggcatcga aaggggcgat tgattcgtta accaccggac tatcgctgga
    34921 agtcgccgcg caggggatcc gcgttaactg cgtgcggcca gggtttattt ataccgaaat
    34981 gcacgccagc ggcggcgagc ctggacgcgt cgatcgcgtt aagtcgaaca tccccatgca
    35041 gcgtggtgga caggcagaag aggtcgcgca ggccattgtc tggctactaa gtgataaagc
    35101 ctcttacgtc acgggaagtt ttatcgattt ggcgggcggg aaataaaaca gggaagttgt
    35161 ctgaccggat gcaacaagta ttgcatccgg tacttcatcg acttaaagct tctcgccgtt
    35221 gctggcaatc acctctttgt accagttaaa gctcttctta cgtgaacgcg acatatcgcc
    35281 agtaccgtcg tcatgtttat tcacatagat aaagccgtag cgtttgctgt actgcccggt
    35341 agtgaatgac acgcagtcga tgcagcccca cggtgtgtag cccatcagat ccacaccatc
    35401 gtaagtcact gcttttttca tctcttcaat atgggcgcgc aggtagtcaa tgcggtagtc
    35461 gtcgttgatg ctgccatctt cttccacttt gtcgtaagcg ccaaaaccgt tttcgacaat
    35521 aaacagcggc ctctgataac gctcatacag ttcgcaaagg gcatagcgca gacctactgg
    35581 atcaatctgc cagccccagt cagacgcttt aacatacggg ttcggtacgc tgccttcaaa
    35641 accagagatc gcatcgccgg tgccgccttc ggccttcact gcgttggtca tgtaatagct
    35701 gaaaccaaga taatcgcagg tgccttcgcg cagtacatcg agatcgccgt cttccatttt
    35761 gatattaaat ccgcgacgtt cccactcgtt caatacatag gacgggtaat agccgcatag
    35821 ctgcacatcg gtaaagacgt agcgttcgcg catcgactcc tgagcgaaca tcacatcgtc
    35881 cgggttacat gagtagggat agagcggcac catcgccagc atacagccga ctttcatctc
    35941 cgggttaatg cgacgcgcgg ctttcactgc cagggcgctg gcgacaaact ggtgatgcag
    36001 cacctggtac atcgtctctt ccgggttgtc atgctcggta tacaccacgc cggagcagca
    36061 gtaaccaaac agcggcgcac gccagttacg ctggttgttt atttcgttga aggtcatcca
    36121 gtatttgact ttatgcttgt agcgttcgaa taccacttcc gcgaaacgga caaagaaatc
    36181 aacgaattta cggttggtcc agctaccgta ttgctgcacc agatgcagcg gcatttcaaa
    36241 gtgggagagg gttatcaccg gttcgatgtt gtatttcagc agttcatcga acagagagtc
    36301 gtaaaacttc agcccttctt cgtttggctg agcttcatca ccttttggaa aaatacgcgt
    36361 ccaggcgatg gaagtccgaa aacatttgaa gcccatttcg gcaaatagct tgatgtcttc
    36421 cttgtagtga ccataaaaat caacggcttc atggtttgga tagtattttc ctggcaagac
    36481 ttctttggtg atttcgcgcg gcacgccgtg tgcgccaccg gtcagaacgt cacaaatgct
    36541 cggtcctttg ccgcctttgt tccagccgcc ttcgacctga tgagcggcaa cggcgccacc
    36601 ccataagaaa tctttcggta aggtgagttt tttcactatc ataggctcca gatcagtcaa
    36661 ttcttacagg aaatagtacc atgcagccaa acgacatcac ttttttcaac gtttccagga
    36721 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
    36781 aacgggtgat gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga
    36841 agtcaccgca acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga
    36901 aaatatgacc ctgcctgaac tgaaaaaggt cattgctgac atctatcccg gtcagacaca
    36961 attttatatg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
    37021 cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac
    37081 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
    37141 ctctggagta agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg
    37201 aaattgccaa cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt
    37261 tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc
    37321 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
    37381 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg
    37441 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
    37501 tgattgttat ctggagccta gcattgctgg ggattctgtt caaactgacc atcgcgcacc
    37561 gattcaaaat tttatctctg gtgacttatc tggcgatggg ctggctgtcg ctggtggtaa
    37621 tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg
    37681 tggtttattc gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca
    37741 tctggcacgg cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata
    37801 ttgggcaggc gtaatgggta tgcctttgta ggcctgataa gacgcgtcag cgtcgcatca
    37861 ggcatcggag cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt
    37921 ccctgttgtg ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag
    37981 gcgataaatg cctgatgcga tacagagatt tactcttcga gcgaatacgg cagcggctcg
    38041 atatgcagtg tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta
    38101 ttcatgacga cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta
    38161 ccggtacgac gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt
    38221 cggctggcac tacctgccag caaccagagc gcgcgtttgt tggcaccacg gaattttgct
    38281 cgcgccacca tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc
    38341 tggagattgg tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa
    38401 ccagcttcaa tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc
    38461 agtttatcgg tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc
    38521 gggtgttcaa accatagcaa agtggtcgcg ccttctttga ctacctgttt ttctttcgaa
    38581 ggcagttcgc taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc
    38641 agcacacgct cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt
    38701 tcagtcagct gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg
    38761 cggaacagac gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg
    38821 tgctgatctt ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg
    38881 ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt
    38941 aaacgggcag aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc
    39001 tgagttgctt aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg
    39061 ctttcactac gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga agcccttgaa
    39121 ttgttgtgaa ttaatttcgc gatcgcgatg gtgtttactg atattcccgt aaaagaagtc
    39181 gaaaaacacg ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat
    39241 taacaacaaa gcccgaattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc
    39301 aatcatgccg tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt
    39361 tattcgtctg ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa
    39421 accggccgat gcagaactgg aaataatggt ccgactcatc cagacacgga accgggaacg
    39481 tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga
    39541 ttcatgggct ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt
    39601 tatggatggt gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggtgtatta
    39661 atgctcgcca gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg
    39721 agtggagcat cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt
    39781 ctgatggcgg taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat
    39841 ggcgggattt acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa
    39901 gcatatgcgc taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt
    39961 tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc
    40021 gccagaccca cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat
    40081 ccttccgttg gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc
    40141 ataccgagcg catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc
    40201 gcaataattt taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct
    40261 tcctggattt caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc
    40321 attaatttcg gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata
    40381 tcgcggattt tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc
    40441 atattcataa cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc
    40501 gctttagtca cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag
    40561 tgaaacagga gctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc
    40621 tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc
    40681 atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag
    40741 aacggctgga gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc
    40801 gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg
    40861 cttcaccgaa attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt
    40921 tattacaggc gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag
    40981 tgttgtatgc taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca
    41041 gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt
    41101 taggggaaga ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc
    41161 tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac
    41221 agaccttctg gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc
    41281 tgtcaccgca tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt
    41341 tacgcgtggt gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc
    41401 atgtcgctac cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc
    41461 tgaaaagaac gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt
    41521 tttcaggctt tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca
    41581 tcaaaagcag cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca
    41641 gcggtgtgtt gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg
    41701 ttagtggcac ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc
    41761 ttgaaaaaga gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta
    41821 ctgatattac ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc
    41881 tggggatcac cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga
    41941 aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg
    42001 caggtaaaag cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg
    42061 gcgtccagct tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc
    42121 cgggttacca gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca
    42181 gcggtaaata attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac
    42241 ggcagacttg cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt
    42301 acgcagtgcg caagaactgg aacgcagtgt taaaggtatg ttgccctggc agcaactaag
    42361 cggtgtcgaa aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat
    42421 tgtggtcggt gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat
    42481 gcgctcgctt ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta
    42541 cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac
    42601 ggtggataac ggtatttcct cccatgcggg agttgagcac gctcgctcgt tgggcatccc
    42661 ggttattgtt accgatcacc atttgccggg cgacacatta cccgcagcgg aagcgatcat
    42721 taaccctaac ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc
    42781 gttttatctg atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg
    42841 cggcatcgca attcctaacc tggcagaact gctggatctg gtagcactgg ggacagtagc
    42901 ggacgtcgtg ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat
    42961 ccgtgccgga aagtgccgtc cagggattaa agcgctgctg gaagtggcaa atcgcgatgc
    43021 acaaaaacta gccgccagcg atttaggttt tgcgctggga ccgcgtctca atgctgccgg
    43081 gcgactggat gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca tcggcgaagc
    43141 gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg
    43201 aatgcaggtt gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctact
    43261 cggcgggctg gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc
    43321 gcgtatcaaa gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac
    43381 gctgaaaggt tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt
    43441 agacacactc taccccggca tgatgctcaa gtttggcggt catgcgatgg cggcgggttt
    43501 gtcgctggaa gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga
    43561 gtggctggcc ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc
    43621 cgaaatgacc atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt
    43681 cccggagccg ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg
    43741 tcatttgaag gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt
    43801 taatgtcgat accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttataa
    43861 gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg
    43921 gccaatttag cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt
    43981 atcaccgcat tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt
    44041 aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact
    44101 acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct
    44161 ggaacgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg
    44221 tcgacaccct cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg
    44281 ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg
    44341 aagaaaaact ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg
    44401 actgttacct cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca
    44461 tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg
    44521 aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt
    44581 acgcttacgg ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt
    44641 ttgactccgg cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg
    44701 atgatgatat tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt
    44761 ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc
    44821 cgaccgggat cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg
    44881 ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga
    44941 aacaggcgat ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg
    45001 tccttgatga ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg
    45061 ccgtgctgga cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag
    45121 gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg
    45181 aactgaaaac gcgtcgtgag aagctggcga acctgcgtga gcagggaatt gccttcccga
    45241 acgatttccg tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga
    45301 acgaagaact ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc
    45361 gtattatggg taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt
    45421 acgttgcccg tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc
    45481 tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta
    45541 tccactgtac cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc
    45601 acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg
    45661 atgaatcccg taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca
    45721 tggtgaaccg cggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg
    45781 ccgctgcgcg tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta
    45841 tcgcgccgga actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa
    45901 tcaaccgtaa cttccgtaac gaaggtattt ccgtgcgtca taacccagag ttcaccatga
    45961 tggaactcta catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt
    46021 tccgtactct ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc
    46081 tggacttcgg taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc
    46141 cggaaaccga catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta
    46201 tcggtatcca cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag
    46261 aagtggcaga agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt
    46321 ctccgctggc gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca
    46381 tcggtggtcg tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc
    46441 aacgcttcct ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg
    46501 atgaagatta cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg
    46561 gcatcgaccg tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt
    46621 tcccggcgat gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc
    46681 ggggtttttt atttaagctg ggtaaatgca gataatcgta ttctggcttc gcgatttgtc
    46741 gcctgcatca ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca
    46801 tcagctaaat cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg
    46861 gtggtgcgtg cggcaaatac cggacacact tcattttcca caatgcctct cggatcggtg
    46921 gcgcggtagc gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca
    46981 taacggcaac ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg gtgcccacaa
    47041 accgagttag tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat
    47101 aattgcccct tggcattaaa cagccaactg gagaacgcga gatgtaagag ggtgtctgcc
    47161 gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg
    47221 acgtgttccg tttgcataat ttctcacatg taattctgat cactcatcat tatagataat
    47281 tgaagagtga aatgattaac caagagagcg tttatcgaca accgcccgtt ttgagcggat
    47341 gccgacaggt aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg
    47401 ccattgcaac acagtcagaa atgacaggaa tagagagagt aaaaagcaac aacagactga
    47461 atatagcgcg cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc
    47521 atcagaagcc acatacgtac tgtcctgact tctaaatctt tatcagagac ggattccttc
    47581 accaggtcag catgatgctg atagccatta aagaagacgt tcagcacaac ggcgctaagg
    47641 gttgcgagca taatgccgct atgtagcagc ggttgcagta cggccggtaa cttagaaaag
    47701 aaatcgtgag agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg
    47761 ttgtaacggt tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc
    47821 atgccgaaca tcactagacc agcgccgccc agcacaaatt gcggaatgga ggctaccagc
    47881 accgccattt ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc
    47941 cagcggctat gaacgcgcat cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg
    48001 gggaagctgt taaacgtgcc gcctatcatt gtccctacgc catcgacacg cagcccgcaa
    48061 ataatatcgt gtgaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc
    48121 atcgactcga taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg
    48181 aaaatcggca taccaaacga catcggggtg acaatcgcaa accacgaggc atcatgtagc
    48241 ccggataaat tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt
    48301 aatacggcga cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt
    48361 aagacggcaa aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct
    48421 ccagcggccc agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca
    48481 ccggtaacca gtggcgggaa taaaggcatc aagcgaccaa taagtggcgc taataatgtg
    48541 gtgataaaac ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc
    48601 atcccaatgg ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg
    48661 cggatcccca taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa
    48721 aagagatccg agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca
    48781 atcataagag gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt
    48841 atgatcagct ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca
    48901 gaagatgagg gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag
    48961 aataaaaagc aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag
    49021 tttttgcgag gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt
    49081 aaaccacgaa aatgacaaga gaacctgaca atggaattat ctgt
//
LOCUS       DAJVPP010000015        49043 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVPP010000015 DAJVPP010000000
VERSION     DAJVPP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.015"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1012)
                     /gene="setB"
                     /locus_tag="OQM34_000888"
     CDS             complement(<1..1012)
                     /gene="setB"
                     /locus_tag="OQM34_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1451438.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQ"
     gene            1380..2510
                     /gene="fruB"
                     /locus_tag="OQM34_000889"
     CDS             1380..2510
                     /gene="fruB"
                     /locus_tag="OQM34_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1451439.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2510..3448
                     /gene="fruK"
                     /locus_tag="OQM34_000890"
     CDS             2510..3448
                     /gene="fruK"
                     /locus_tag="OQM34_000890"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1451440.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3465..5156
                     /gene="fruA"
                     /locus_tag="OQM34_000891"
     CDS             3465..5156
                     /gene="fruA"
                     /locus_tag="OQM34_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1451441.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5580..6521
                     /locus_tag="OQM34_000892"
     CDS             5580..6521
                     /locus_tag="OQM34_000892"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1451442.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6509..7447
                     /gene="psuG"
                     /locus_tag="OQM34_000893"
     CDS             6509..7447
                     /gene="psuG"
                     /locus_tag="OQM34_000893"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1451443.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7541..8791
                     /locus_tag="OQM34_000894"
     CDS             7541..8791
                     /locus_tag="OQM34_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1451444.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8862..9556)
                     /gene="yeiL"
                     /locus_tag="OQM34_000895"
                     /pseudo
     CDS             complement(8862..9556)
                     /gene="yeiL"
                     /locus_tag="OQM34_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9687..10628
                     /gene="rihB"
                     /locus_tag="OQM34_000896"
     CDS             9687..10628
                     /gene="rihB"
                     /locus_tag="OQM34_000896"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS1451445.1"
                     /translation="MEKREIILDCEPGHDDAIAMMMAAKHSAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10728..11978
                     /locus_tag="OQM34_000897"
     CDS             10728..11978
                     /locus_tag="OQM34_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1451446.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTAQVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIARLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12034..13122)
                     /locus_tag="OQM34_000898"
     CDS             complement(12034..13122)
                     /locus_tag="OQM34_000898"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1451447.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLSELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13125..13982)
                     /gene="nfo"
                     /locus_tag="OQM34_000899"
     CDS             complement(13125..13982)
                     /gene="nfo"
                     /locus_tag="OQM34_000899"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS1451448.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRIDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14056..15105)
                     /locus_tag="OQM34_000900"
     CDS             complement(14056..15105)
                     /locus_tag="OQM34_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1451449.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15204..16085
                     /locus_tag="OQM34_000901"
     CDS             15204..16085
                     /locus_tag="OQM34_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1451450.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16291..17760
                     /gene="lysP"
                     /locus_tag="OQM34_000902"
     CDS             16291..17760
                     /gene="lysP"
                     /locus_tag="OQM34_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1451451.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18052..20043
                     /gene="cirA"
                     /locus_tag="OQM34_000903"
     CDS             18052..20043
                     /gene="cirA"
                     /locus_tag="OQM34_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCS1451452.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSDTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20075..20910)
                     /gene="yeiG"
                     /locus_tag="OQM34_000904"
                     /pseudo
     CDS             complement(20075..20910)
                     /gene="yeiG"
                     /locus_tag="OQM34_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21168..21836
                     /gene="folE"
                     /locus_tag="OQM34_000905"
     CDS             21168..21836
                     /gene="folE"
                     /locus_tag="OQM34_000905"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1451453.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21853..22929
                     /locus_tag="OQM34_000906"
     CDS             21853..22929
                     /locus_tag="OQM34_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1451454.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23071..24111
                     /gene="galS"
                     /locus_tag="OQM34_000907"
     CDS             23071..24111
                     /gene="galS"
                     /locus_tag="OQM34_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCS1451455.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24391..25389
                     /gene="mglB"
                     /locus_tag="OQM34_000908"
     CDS             24391..25389
                     /gene="mglB"
                     /locus_tag="OQM34_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1451456.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25450..26970
                     /gene="mglA"
                     /locus_tag="OQM34_000909"
     CDS             25450..26970
                     /gene="mglA"
                     /locus_tag="OQM34_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1451457.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            26986..27996
                     /gene="mglC"
                     /locus_tag="OQM34_000910"
     CDS             26986..27996
                     /gene="mglC"
                     /locus_tag="OQM34_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1451458.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28032..28382)
                     /locus_tag="OQM34_000911"
                     /pseudo
     CDS             complement(28032..28382)
                     /locus_tag="OQM34_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(28472..28879)
                     /locus_tag="OQM34_000912"
     CDS             complement(28472..28879)
                     /locus_tag="OQM34_000912"
                     /inference="COORDINATES: protein motif:HMM:NF013355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1451459.1"
                     /translation="MIECNFSCPQMTSHAMGSDVGQSPELVEKYCRAVKRGSTLPMLA
                     KMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKSSISGYSG
                     KAVKPIALRFIQQMRTHPELRDFPISGIGGIET"
     gene            complement(28876..29244)
                     /locus_tag="OQM34_000913"
     CDS             complement(28876..29244)
                     /locus_tag="OQM34_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCS1451460.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEQLARI"
     gene            complement(29238..30476)
                     /gene="preT"
                     /locus_tag="OQM34_000914"
     CDS             complement(29238..30476)
                     /gene="preT"
                     /locus_tag="OQM34_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCS1451461.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGGKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30670..30909)
                     /locus_tag="OQM34_000915"
     CDS             complement(30670..30909)
                     /locus_tag="OQM34_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1451462.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(30912..31631)
                     /gene="sanA"
                     /locus_tag="OQM34_000916"
     CDS             complement(30912..31631)
                     /gene="sanA"
                     /locus_tag="OQM34_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1451463.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31781..32665)
                     /gene="cdd"
                     /locus_tag="OQM34_000917"
     CDS             complement(31781..32665)
                     /gene="cdd"
                     /locus_tag="OQM34_000917"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1451464.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32795..33490)
                     /locus_tag="OQM34_000918"
     CDS             complement(32795..33490)
                     /locus_tag="OQM34_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1451465.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33487..33885)
                     /locus_tag="OQM34_000919"
     CDS             complement(33487..33885)
                     /locus_tag="OQM34_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1451466.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34124..35071
                     /gene="dusC"
                     /locus_tag="OQM34_000920"
     CDS             34124..35071
                     /gene="dusC"
                     /locus_tag="OQM34_000920"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS1451467.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35442..35525)
                     /gene="yohP"
                     /locus_tag="OQM34_000921"
     CDS             complement(35442..35525)
                     /gene="yohP"
                     /locus_tag="OQM34_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS1451468.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35749..37185
                     /gene="mdtQ"
                     /locus_tag="OQM34_000922"
     CDS             35749..37185
                     /gene="mdtQ"
                     /locus_tag="OQM34_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCS1451469.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37238..37999
                     /locus_tag="OQM34_000923"
                     /pseudo
     CDS             37238..37999
                     /locus_tag="OQM34_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38116..38694)
                     /locus_tag="OQM34_000924"
     CDS             complement(38116..38694)
                     /locus_tag="OQM34_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1451470.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            38864..39451
                     /locus_tag="OQM34_000925"
     CDS             38864..39451
                     /locus_tag="OQM34_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1451471.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39625..40557
                     /gene="pbpG"
                     /locus_tag="OQM34_000926"
     CDS             39625..40557
                     /gene="pbpG"
                     /locus_tag="OQM34_000926"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1451472.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40595..42310)
                     /gene="dld"
                     /locus_tag="OQM34_000927"
     CDS             complement(40595..42310)
                     /gene="dld"
                     /locus_tag="OQM34_000927"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1451473.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42506..44803
                     /gene="bglX"
                     /locus_tag="OQM34_000928"
     CDS             42506..44803
                     /gene="bglX"
                     /locus_tag="OQM34_000928"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1451474.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45055..45972
                     /gene="osmF"
                     /locus_tag="OQM34_000929"
     CDS             45055..45972
                     /gene="osmF"
                     /locus_tag="OQM34_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS1451475.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            45979..47136
                     /gene="yehY"
                     /locus_tag="OQM34_000930"
     CDS             45979..47136
                     /gene="yehY"
                     /locus_tag="OQM34_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS1451476.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47129..48055
                     /gene="yehX"
                     /locus_tag="OQM34_000931"
     CDS             47129..48055
                     /gene="yehX"
                     /locus_tag="OQM34_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1451477.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48060..48791
                     /gene="yehW"
                     /locus_tag="OQM34_000932"
     CDS             48060..48791
                     /gene="yehW"
                     /locus_tag="OQM34_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1451478.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48772..48879)
                     /locus_tag="OQM34_000933"
     CDS             complement(48772..48879)
                     /locus_tag="OQM34_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1451479.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(48939..>49043)
                     /locus_tag="OQM34_000934"
     CDS             complement(48939..>49043)
                     /locus_tag="OQM34_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1451480.1"
                     /translation="RTLLVWCGENRTSAQQQQLTSWQEQGHDIFPLGI"
BASE COUNT        11845 a        12074 c        13166 g        11958 t
ORIGIN      
        1 cctgaccggg catcaaatcc tgaaaataga gcatcccgat gccgcccaga atgccaataa
       61 aaatagcatt tagcagctgc aagcccaaca gaatggcagg tgaatgcgcc atcagcattc
      121 ctgcgtaaaa acagacgcca cccacggcag caacgcgcat taagaaacgc ttacccagac
      181 gtttggcgaa atatccggca atcaacatcg tcgggatttc cagcccggcg gcggtcccca
      241 tcatcacacc ggccagtttc tcgggaagat gcagttcgtt gataataaat agcggcatgt
      301 tgatgatgta caggctgttc gagccccaca tcaatgtaca aatgacaaac agcagtagcg
      361 tatcgcgacg gttacggcgc ggcgcttcaa ccgtgccggt cgccagcgga agctcttttt
      421 gcatcgacgg taaaaacagc cacaccatca cgccgcaaac aataaacgct accgctgcgc
      481 tcagatacat taccgtaaag ctgaaaccca tcgctaacgc ataagccagc ggtgggccaa
      541 tgacccatgc cagtgaaacc tgagcgcgta aaaaagagct gaacatcacc gcctcacgtc
      601 cggttttgtc ggcatgttca cgggcaaggg caaacatttg cgggttagcg gtcgagccaa
      661 agctgctaag aaagacgcca acgaatagca aaacaaagta gttgcgattc caagcaaaaa
      721 gggtgcaggc cagcacgcct aacaggcagc aaaagacaat cagcgatttg cgatcgccgc
      781 gcttatcaga gcgcccagcg agaaactgac ttaccagaat cccaatgaca gcgctgccgg
      841 taaagaagaa tcccaccatc gccggacggg catgtacttc atcggtaagg aaaatactga
      901 gtgtcggggt ttgcagagca cccgcaatac cggtgagaaa ggcaacgatt aaaaacgccg
      961 tcgaggtcag gtcaaacgat ttcgcgctgg agactgcggg ggagttatgc atgctggtcg
     1021 atcactggaa gaggaagacg cggagtttac gctgcttgtc agaaaataaa cagacagcga
     1081 aatcaaaagc gccacgaata atcaaaatgc catttcactg ttgctgattg gctgctgacg
     1141 ctgctgaaat gaaattgctg ataacggatt ttcccatcag caattaggaa aaatgacaaa
     1201 aaattgtgca gcacatcaaa cttttgctca taactttacg gctttccttg cgtgctgaaa
     1261 cagaagtatt atgctttctt gaaacgtttc agcgcgatct tgtctttaac cctaagctag
     1321 gttggcgctt tttttctcat agaggctgaa tcgtttcaat tcagcaagag aggagaacta
     1381 tgttccagtt atccgtacag gacatccatc cgggcgaaaa ggccggagac aaagaagagg
     1441 cgattcgcca ggtcgctgcg gcgctggtgc aggccggtaa tgtagcagaa ggctacgtca
     1501 atggcatgct ggcgcgcgaa cagcaaacct caacgttcct cggcaatggt attgctattc
     1561 cacacggcac taccgacacc cgcgatcagg tgctgaaaac cggcgttcag gtatttcagt
     1621 tcccggaagg cgtcacctgg ggtgacggtc aggtagcgta cgtggcaatc ggtattgctg
     1681 ccagctcgga taaacatctg ggcctgctac gccagctgac ccacgtactg agcgatgatt
     1741 ccgttgctga acaactgaag tcagcaacaa cagcagaaga acttcgcgca ttactgatgg
     1801 gcgaaaagca gagtgagcag ctgaagctcg acaacgaaat gctgacgctg gatatcgtcg
     1861 ccagcgatct gctgactctt caggcgctga acgctgcgcg tctgaaagag gcgagggcag
     1921 ttgacgccac tttcgtcacc aaagccatca atgaacaacc gctgaatctc ggacagggta
     1981 tctggctgag cgatagcgcc gaaggcaatc tgcgtagtgc gattgcggta agccgtgcgg
     2041 caaatgcttt tgatgtggac ggcgaaacgg cagccatgct ggtgagtgtg gcgatgaatg
     2101 acgatcagcc catcgcggtt cttaagcgtc tcgctgattt gttgctcgac aataaagctg
     2161 accgcttgct gaaagcggat gcggcaacgt tgctggcgct gctgaccagc gatgatgcgc
     2221 cgaccgacga cgtgttaagc gcggagtttg tggtgcgcaa tgaacacggc ctgcatgctc
     2281 gtccaggtac catgctggtc aataccatta aacaatttaa cagtgatatt accgtgacaa
     2341 accttgatgg caccggcaaa ccggcaaacg gacgtagtct gatgaaagtt gtggcacttg
     2401 gcgttaagaa aggtcatcgc ctacgcttta ccgcccaggg tgcagatgct gaacaggcgc
     2461 tgaaagccat tggcgacgct atcgctgctg gtcttgggga gggcgcataa tgagcagacg
     2521 tgttgctacg atcaccctta atccggctta tgaccttgtt ggtttctgcc cggaaattga
     2581 acgcggcgaa gtgaacctgg tgaaaaccac cggtctgcat gcggcgggta aaggcatcaa
     2641 cgtggccaaa gtattaaaag acctgggaat tgatgtcacc gttggcggct tcctaggtaa
     2701 agacaatcag gatggttttc agcaactgtt cagcgagctg ggcattgcca accgtttcca
     2761 ggttgtacag gggcgcaccc gaattaacgt taagctgacg gaaaaagacg gcgaagtgac
     2821 cgacttcaac ttctcgggtt tcgaagtcac ccccgccgac tgggaacgct ttgtgactga
     2881 ttctctgagc tggctcggtc agttcgatat ggtctgtgtc agcggaagct taccgtcagg
     2941 cgtcagcccg gaagcgttca ccgactggat gactcgcctg cgtagtcagt gtccttgcat
     3001 tatctttgat agtagccgtg aagcgttagt agcaggtttg aaagcggcac cgtggctggt
     3061 gaaacctaac cgccgcgagt tggaaatctg ggcaggccgt aaactgcctg aaatgaaaga
     3121 tgtgattgaa gctgcacatg cgctacgtga acaaggtatt gcgcatgttg ttatttcact
     3181 gggtgccgaa ggcgcgcttt gggttaatgc ctccggcgaa tggatcgcta aaccaccgtc
     3241 agtcgatgtc gtaagcaccg ttggcgcagg ggattctatg gttggtggcc tgatttatgg
     3301 cttgctgatg cgtgaatcca gtgaacacac actgcgtctg gcgacagctg ttgcagccct
     3361 ggcggtaagt caaagcaatg tgggtattac cgatcgtccg cagttggccg caatgatggc
     3421 gcgcgtcgac ttacaacctt ttaactgaca gcaggagagg cataatgaaa acgctgctga
     3481 ttattgacgc taatctcggt caggcacgcg cctatatggc gaagaccctg ctgggcgcgg
     3541 cggcgcgaaa agcaaaactg gaaatcatcg acaatccgaa cgacgcagaa atggcgattg
     3601 ttctcggtga ttccatcccg aatgacagcg cgctgaacgg taaaaatgtc tggctgggtg
     3661 atatttcccg ggcagttgcg caccctgagc tgttcctgag tgaagccaaa ggccatgcga
     3721 aaccttacac tgcgccggtc actgcgacag caccggttgc cgccagcggt ccgaaacgcg
     3781 tagttgcggt gactgcttgc ccgactggcg tagcacacac ctttatggcg gctgaagcca
     3841 ttgaaaccga agcgaaaaaa cgtggctggt gggtgaaagt tgaaacccgt ggttctgttg
     3901 gcgcgggtaa tgcaatcact cccgaggaag tagccgcagc ggatctggtg attgtggcgg
     3961 cagatatcga agtggatctg gcgaaatttg ctggtaaacc gatgtatcgt acctctaccg
     4021 gtctggcgct gaagaaaacc tcgcaggaac tggataaagc ggttgctgaa gcaacgccgt
     4081 atgaaccggc gggcaaagct caaacggcga ccactgaagg taagaaagag agtgcaggcg
     4141 cttatcgtca cttgctaacg ggcgtctctt acatgctgcc gatggtcgtt gctggtggtc
     4201 tgtgtatcgc gctttctttt gcttttggta tcgaagcgtt taaagagccg ggtacgttgg
     4261 cagcggcgct gatgcagatt ggtggtggtt cagcctttgc gctgatggtg ccggtactgg
     4321 caggttatat tgccttctcc attgccgatc gtccgggcct cacgccgggt ctgattggcg
     4381 gtatgctggc ggtcagcacc ggttctggct tcattggcgg tattattgcg ggcttcctgg
     4441 ctggttacat tgcgaagtta atcagtacgc aactgaaact gccgcagagt atggaggcgc
     4501 tgaaaccgat cctgatcatt ccgctaattt ccagtctggt ggtcggtctg gcgatgatct
     4561 acctgatcgg taaaccagtt gctggcattc tcgaagggct gactcactgg ctgcagacca
     4621 tggggactgc gaatgcggtt ctgctggggg cgatcctcgg tggcatgatg tgtactgaca
     4681 tgggcggtcc ggtaaacaaa gcagcgtacg cattcggtgt gggtctgctg agtactcaaa
     4741 cctatggccc gatggcggcg attatggcgg caggtatggt gccaccgctg gcaatgggcc
     4801 tggcaacaat ggtggcgcgt cgcaaattcg acaaagcgca gcaggaaggt ggcaaagccg
     4861 ctctggtatt gggactgtgc ttcatttcgg aaggtgcaat tccgtttgct gctcgtgatc
     4921 cgatgcgtgt gctgccgtgc tgtatcgtgg gtggggcgct gactggcgca atctcaatgg
     4981 cgattggtgc gaaactgatg gcaccgcacg gtggtctgtt tgttctgctg atccctggcg
     5041 ctattacgcc ggtattgggt tacctggtag caattattgc cggtacgctg gtggcgggtt
     5101 tggcctatgc cttcctgaaa cgtccggaag tggacgcagt agcgaaagca gcgtaataaa
     5161 aggtgtgtta cagggcagaa atttcctgcc ctgtaacatt tgatgagcgc aaagagaatg
     5221 accagcttgt gaatgtaatt ttatgaattt aaatggtttt atctttcagt agaaacttca
     5281 aacgttcatc aaacactaag gttatttcct caagggcaga ttattttctg ccctttttta
     5341 gtgttcctgt tctgtgcatc ccgtcacaaa ttcctcgctt ttgtaattca ggcatttaat
     5401 cgcattttgt gagcctgctc aaatctgccc attttgcctc tctggcggta atttacttta
     5461 cgtaaaacat gcatactatg agcacatgtt taaatatgaa cacatgttta aaccgtcttt
     5521 gcagtatcta tattcttgtg gattgacagg gttggtaatt ttttaccaga ggacgtgcta
     5581 tgcgcgaaaa ggattatgtc gtaattatag gttcggcgaa tattgatgtc gccggatatt
     5641 cacatgaatc attaaattat gcggattcaa atccaggtaa aataaaattt acgcctggtg
     5701 gagtagggcg caatattgca caaaacctgg cgttgctggg taacaaagcc tggctactga
     5761 gcgccgtagg cagtgatttt tatggtcaat cgctgctaac gcaaaccaat caatctggtg
     5821 tttatgtcga taaatgcctg attgtgccgg gagaaaatac gtcgagttat ttatcattac
     5881 tcgataatac cggtgaaatg ctggttgcta taaatgacat gaatattagc aacgctatta
     5941 cagctgaata tctcgcacag caccgtgaat ttattcagag ggcaaaggtc attgtcgcgg
     6001 actgtaatat cagtgaagag gcactggcat ggattctgga taatgccgcc aacgtacccg
     6061 tatttgtcga tccggtttca gcgcggaaat gtgtcaaagt gcgcgaccgt ctaaatcaga
     6121 tccacactct gaagccaaac cgccttgaag cggaaaccct gagtgggatt gcgctgtcag
     6181 ggcgtgacga tgtggcaaaa gttgctgcct ggttccatca acatggcctg aaccgactgg
     6241 tattgagcat gggcggcgac ggcgtttatt acagcgatat cagcggtgaa aatagctggt
     6301 ctgcgccgat caaaaccaat gttattaatg ttaccggagc gggcgatgcc atgatggcgg
     6361 gacttgcttc gtgttgggta gacggaatgc cgtttgccga atctgttcgt ttcgcacagg
     6421 gatgttcgtc aatggcgctc tcctgtgaat acaccaataa ccccgattta tcgattgcca
     6481 acgttatatc gttagtggag aacgcagaat gtctgaatta aaaatctccc ctgaattatt
     6541 acaaatttct ccggaagtgc aggacgcttt aaaaaacaaa aaaccggttg tggcgctgga
     6601 atcgaccatt atttctcacg ggatgccgtt cccacaaaat gcccagaccg caattgaagt
     6661 agaagaaact attcgtaaac agggcgcagt acctgccact atcgccatta ttggcggcgt
     6721 gatgaaagtg ggtttaagca aagaaaaaat tgaattactg ggtcgtgaag ggcataacgt
     6781 gaccaaagtt agtcgtcgcg atttaccttt tgttgttgcc gccggaaaaa atggcgcaac
     6841 cactgtggct tcaacgatga ttattgcggc gcttgccgga attaaagtat ttgccaccgg
     6901 gggaattggt ggtgtgcatc gcggggcgga acataccttc gatatttctg ccgatttgca
     6961 agaactggca aatactaatg tcaccgttgt ttgtgccggg gcgaaatcta ttctcgattt
     7021 aggattaacc actgagtatt tagaaacctt cggtgtgccg ttaattggct atcagactaa
     7081 agcgctgcct gcgtttttct gccgtaccag ctcgtttgac gtcagcattc gtctcgacag
     7141 cgccagtgaa attgcccgtg caatggcggt gaaatggcaa agcgggctga acggtggcct
     7201 cgtggtagcg aacccgatcc cggaacagtt tgcgatgccg gaggaatcta tcaatgcagc
     7261 catagatcaa gccgtcgccg aagccgaaga gcagggcgtt attggtaaag aaagtacacc
     7321 gttcctgctg gcgcgcgttg ctgagctgac cggcggtgac agcctgaaat ccaatatcca
     7381 gctggtgttc aacaacgcca ttctggcgag tgaaattgcc aaagaatacc agcgtctcgc
     7441 gggttaattt ctgacgggca gaaagccgtc gcccgtactc atcaagcata ccaaaacctg
     7501 gcgctgacgc cgggtttact ggcatgaagg gaaaatcatt atggatataa tgagaagtgt
     7561 tgtggggatg gtggtgttac tggcaatagc atttctgttg tcagtgaata aaaagagcat
     7621 cagtttgcgc acggttggag ccgcactgct gctgcaaatc gctattggtg gcatcatgct
     7681 ctacttccca ccgggaaaat gggcagtaga acaggcggca ttaggcgttc ataaagtgat
     7741 gtcttacagt gatgccggta gcgccttcat ttttggttcg ctggttgggc cgaaaatgga
     7801 tgtcctgttt gacggtgcgg gttttatctt cgcctttcgc gtacttccgg cgattatttt
     7861 cgttactgcg ctcatcagtc tgctgtacta cattggcgtg atggggctgc tgattcgcat
     7921 ccttggcagc attttccaga aagccctcaa catcagcaaa atcgaatctt ttgttgcggt
     7981 taccactatt ttcctcgggc aaaatgagat cccggcgatc gttaaaccgt ttatcgatcg
     8041 cgtgaatcgc aacgagttgt ttaccgcaat ttgtagcggg atggcgtcca ttgctggttc
     8101 ggtgatgatt ggttatgccg gaatgggcgt accaattgac tacctgttag cggcatcgct
     8161 gatggcgatc cccggcggta ttttgtttgc acgtattctt agcccggcaa ccgagccttc
     8221 gcaggtcaca tttgaaaatc tgtcgttcag cgaaacgccg ccaaaaagct ttatcgaagc
     8281 ggcggcgagc ggtgcgatga ccgggctgaa aatcgccgct ggtgtagcga cggtggtaat
     8341 ggcgtttgtc gcaattattg cgctgatcaa cggcattatc ggcggaattg gcggctggtt
     8401 tggtttcgcc aatgcctctc tggaaagtat ttttggctat gtgctggcac cgctggcgtg
     8461 gatcatgggt gtggactgga gtgatgccaa ccttgcaggt agcctgattg ggcagaaact
     8521 ggcgattaac gaattcgtcg cttacctgag tttctcccca tacctgcaaa cgggcggcac
     8581 gctggaagtg aaaaccgtgg cgattatatc ctttgcgctt tgtggttttg ctaactttgg
     8641 ttctatcggt gttgtcgttg gcgcattttc ggctatttcg ccaaaacgtg ctccagaaat
     8701 cgcccagctt ggtttacggg cgctggcagc agcaacgctt tccaacctga tgagtgcgac
     8761 tattgccggg ttctttattg gtctggcgta atgctcgatt tgtagaccgg ataaaacgta
     8821 ttaagcgttg catccggcaa tcgcgctatt gcaaataatt tttactgcat catcccggag
     8881 aatttattct ccgggtccat ctccagcgcc agtcctgaca actgctttct gtttttaatt
     8941 agatatcctt tcttttaatt aataaaccgt cgtgaatgaa ttgcgcgaga acatataaaa
     9001 gatgtcgata agaaacgcct aaatactctg ccgcttgcgt atgcttttcg tgataaagat
     9061 caccttcttg tgagagtaat ataaatgctg ccaggcgatt cactaacgga aatgattgat
     9121 tctgagttaa agaaacaata ttacgataat ttttatgact taaggtgacg caaagttttc
     9181 gtaaaaatag ggtgtcgttt aataacagcg gacggtaatg tttcataggg agcgcaaggc
     9241 accaacactc ttcaatagcc tgcaccgcac gcggttcatg gtctttgtcg attaactcaa
     9301 tctcgccaat aaaacaggga gcggcaaaga aatcgatcag cgacacgcga ccattggcaa
     9361 gcgtggtgta aagcctggcg cggcctcgcg tcagataaaa cagccatgac ggctgttgcc
     9421 cttcctgcac aatgtagtca cgcgctaaaa aatgaaacag ccgcgtatcg gctgaaacat
     9481 ccgttgaaaa gcaatcctta aacgcagatt cactcatcaa ttgctgctta aggtcattat
     9541 tgtggatttc tttcacgatg tttatccgct atgagatttc tcatatttag catacgtacg
     9601 ctgttgttaa gattcaatca ttggcccggt cgaattttct ttgctgtaga aaaaaataac
     9661 gcaactggaa acagaggaaa taaacaatgg aaaagagaga aattattctg gattgtgagc
     9721 cgggtcatga tgatgctatt gcaatgatga tggcagcgaa acattcggca atagatttat
     9781 taggcatcac tattgtagcg ggtaatcaga cgcttgataa aacattaatt aatggcctga
     9841 atgtttgcca gaaactggag attaatgttc cggtttatgc ggggatgccg caacccatta
     9901 tgcgtaaaca aatcgttgcc gataatattc acggtgaaac cggactggat ggcccggtat
     9961 tcgagccgct gacccgccag gcagaaagta cccatgcggt gaaatatatc atcgataccc
    10021 tgatggcaag cgatggcgat atcactctgg tgccggttgg tccgctttca aatatcgcgg
    10081 tggcaatgcg tatgcaaccc gcaatcctgc ccaaaatccg tgaaattgtg ctaatgggcg
    10141 gcgcttacgg tacaggcaac ttcaccccat ctgccgagtt caatatcttt gccgacccgg
    10201 aagccgcccg cgtggtgttc acctccggcg ttccattagt gatgatgggc ctcgatctta
    10261 ccaaccagac cgtttgcacc ccggatgtaa ttgctcggat ggaaagggta ggcggccctg
    10321 ccggagagct gttcagcgac atcatgaact tcactctcaa aacgcagttc gaatactacg
    10381 gcctggctgg cggcccggtg catgacgcca cctgcattgg ttatctgatc aaccctgatg
    10441 gcattaaaac ccaggatatg tacgtcgaag tggacgtcaa cagcggccct tgctacgggc
    10501 gtacggtctg cgacgaactg ggcgttcttg gcaagcccgc taataccaaa gtcggcatca
    10561 ctattgatac cgactggttc tggggcttag tcgaagagtg cgtgcgcggc tacatcaaaa
    10621 cccattaagt gttgataaca agccgggaca gtatcgcaag atgcaggccc ggctatttct
    10681 gatgggcaga aagccgtcgc ccaaaatgac atgaagagat aatcactatg gatgtcatga
    10741 gaagtgttct gggaatggtg gtactgctgg caattgcgtt tttactgtca gtaaacaaga
    10801 agaagatcag cctgcgtacc gttggcgcgg cgttagtgtt acaggtcgtg attggcggga
    10861 ttatgctttg gttaccgcca gggcgttggg tcgcagaaaa agtcgctttt ggcgtccaca
    10921 aagtgatggc gtacagcgac gcaggtagcg catttatctt cggttctctg gtcggaccga
    10981 aaatggatac cttatttgat ggcgcaggat ttatctttgg tttcagggta ttaccggcaa
    11041 ttatcttcgt cactgcacag gtgagtattc tctactacat cggtgtgatg gggatcttaa
    11101 ttcgcattct cggcggtatc ttccagaaag ctttaaatat cagcaagatc gagtcattcg
    11161 tcgcggtcac caccattttc ctcgggcaaa acgaaattcc ggcgatcgtc aaacccttta
    11221 tcgatcgtct gaatcgcaat gaattattta cagcgatttg tagtggcatg gcctcgattg
    11281 ctggttcgac aatgattggt tacgccgcac tgggcgtacc tgtggaatat ttgctggcgg
    11341 catcgttaat ggcgatccct ggcgggatct tgtttgcccg cctgttaagc ccggctacgg
    11401 aatcttcgca ggtttctttt aataacctct ctttcaccga aacaccgcca aaaagcatta
    11461 ttgaagccga tgcgacaggg gcaatgaccg ggctgaaaat cgccgccggt gtagcgacag
    11521 ttgttatggc atttgtcgcc atcattgcgt taattaacgg tattatcggc ggcgttggcg
    11581 gctggtttgg ttttgaacat gcctcgctgg agtccatttt aggttacctg ctggccccac
    11641 ttgcgtgggt aatgggtgtt gactggagtg atgcaaatct tgccgggagt ttgattggac
    11701 agaaactggc aataaatgaa tttgtcgctt atctcaattt ctcaccctat ctgcaaacgg
    11761 ctggcactct ggatgctaaa accgtggcga ttatttcctt cgcgttgtgc ggtttcgcta
    11821 actttggttc tatcggggtg gtggtggggg cgttttctgc ggttgcgcca caccgtgcgc
    11881 cggaaatcgc ccggcttggt ttacgcgcgc tggcggcggc gacactttct aatctgatga
    11941 gtgccaccat tgccgggttc tttattggtt tagcgtagtg tgcaatttat gagccggatt
    12001 agatgcggtc aagcgtcgaa tccggcaaca ttgttacacc attggcacta atgacagcgc
    12061 gttatcggca gacagggtag ggttgtttaa gctgccgctg gcgcgtgaaa ttgccgcgca
    12121 ggccatcgca aaacgggcgc tgtcacggaa attgtttccc tccagaaagc tatataccag
    12181 acccgccata aaaccatcgt cagcaccaaa actgtctacc gtcgtatgcg ctggcacagt
    12241 cagcaaaaat tgttcaccat ccttttcgct gcaataaact gactcatcgg gcaaataaac
    12301 aaacagttgc tgaacacctt gctgatgcaa tgcattcact gcggcattac ggtcagcatc
    12361 gctggtgatc gcctgtcccc ataaaatttc cagctccgat aaagtgggtt tcagggtgtg
    12421 aatatgcgcc agccagtgtt tgattttgcc cgctttgaat tctgaaacgg tatcgacaaa
    12481 caccgggatc tcatcagcga gggtaaagac ccattccagc gcctcggctg tcaggttgca
    12541 atcagccagt accacgcccg catgacgaag taaatcgcgc gacccgttca gtagttgcgg
    12601 tgtcaactgt tccagcagat gggtatcgtt aattgccagc acggtttgat cgtctcgatt
    12661 ggcaattgcc agatacgtcg atgtgctttg accatgcaaa cgaacgcagc cggagacatt
    12721 cacgccggca cggcgcgttt cttccaggag catttcgcca taaaagtcat cgccaatcac
    12781 tgaaagcaaa tggacgtcac ggcctaacag cgccagattg tgggcgatat tgcgtccaac
    12841 gccgcctgcc gaacaatgaa ttgtaccggg atgagaagcc gcttgcgggt aatggatatc
    12901 cgccatcccg cgaatatcca tattgattgt ccccaccact acgcagtatt cctgctcggt
    12961 gagaatgtaa cctttgtctt taatccggcc tttgcgcatt aaatccataa tatgcgccgc
    13021 aacgcgcgaa cgactgattt gcagcatgtc cgcaatttcg ttctgctgaa tcagcgggtt
    13081 acgccgtaaa attgcaagga tctccttttc ccggttattc atcttcaggc taccgctttt
    13141 tcagtttgtt gcgctttcag ccaggcgatc tcttctgccc agatatccgg gttgatggtt
    13201 tcaaggatca gcggaatgcc gtcgaaacgg tcgttctgca taatccagcg gaacgcatca
    13261 tgaccgatat tgccttcacc gaggctatga tggcggtcaa tgcggctgcc aaaggtactt
    13321 ttcgcatcgt taaggtgcat cccgcgcaga tacttaaagc cgacaatacg ggcaaaatca
    13381 gcgaacgttt tttcgcattc ggctggagta cgcaaatcat acccggcagc gaaagcatgg
    13441 caggtatcaa tgcagacgcc gacgcgggat ttatcttcca cgccgtcgat aatcgccgcg
    13501 agatgttcga atttaaaccc taagttactg ccctgaccgg cggtgttttc aatcaccgct
    13561 gttacacctt gagttttatc cagcgcaatg ttgatggatt cggcaatacg cgcaaggcaa
    13621 tcctcttctg aaatctgcat cagatggctg ccagggtgga agttgagcaa agaaagcccc
    13681 agttgttcgc aacgctgcat ttcatctata aaggcatcgc gcgatttttc cagagcttca
    13741 gtgaccggat gtccgaggtt gatcagataa ctgtcgtggg gaagaatttg cgccgatgtg
    13801 tagtgatatt tttcacaggc ggctttgaat tcatcgatgg tttgcgtcgt gagcggtgcg
    13861 gcacgccact gacgttggtt tttggtgaac aaggcaaacg cggttgcgtc gatttcggcg
    13921 gcgcgaattg cggcatttgc cagaccgcca gcagcactaa cgtgcgctcc aatgtatttc
    13981 atgcgaggac tcctgttaaa cccgctggag gaaaacggta atgatagcgg gttaacagga
    14041 gcaagaagta gtggtttatg cgatgacgct ttgaatcaca tagttaatcg caccaccacc
    14101 aacaatcagc caggcaaaca gtaccagtgc catcagcaga ggtttcgccc cagctttttt
    14161 cagcgcgctg acgtgagtgg tcagacccag cgccgccatc gccattgcca gcaggaaggt
    14221 atccagcgtt accagcatgt tcaccacgct ctgcggtaac aggtggaacg agttaaagat
    14281 ggcaactacg atgaacaaga tggcaaacca cggaatagtg attttgcttt tctcgccgct
    14341 gttcgcccca gacagctgtt taacacgcgc cgccagcagg atgaggaacg gagccagcat
    14401 catcacgcgc agcattttgg aaataactgc tgcgttttct gcatccgggc tgatggcatg
    14461 acctgccgcc accacctgcg ccacttcgtg cacagtagaa ccaatgtaga taccgaaagt
    14521 ttccggacta aaccattgag acatcagcgg atatatcgcc gggtagagga aaatcgcgac
    14581 ggtcccgaag ataacaacgg ttgcaacagc cacggttact ttactggctt ccgctttcac
    14641 taccggctca gtcgccagta ccgcggcagc accacagata ctgctaccgg caccgatcaa
    14701 ccaactggtg tgcttatcca gaccaaacac tttctgcccc aggaagcaag ccagcaggaa
    14761 ggtactggac agcgtcagca cgtcaatgat gatcccactg ataccgacat cggcaatttg
    14821 cgagaacgtc agacggaagc cataaagaat gatacccaga cgtaataaat attgcttggc
    14881 aaacagcaca ccaccgtcac agcttttcca gatgtgcgga tagatggtgt tgcctaaaac
    14941 catccccaac aagattgcga gggtgagggc actaaacccg gcacccgcaa ccgcgggaat
    15001 ggaaccaccc cacagggcga ccccggtgat aactgcactc agggctaacc ccggaataaa
    15061 atgccacagt gtacgatgtt gtttctgtaa ggtgatattc gtcataaccc tctcctttaa
    15121 ccgggcataa ggttacgact aactggttta aaaataaaat tgattatata tttataatta
    15181 atctttataa gtggtaagcg actatgcaca tcaccctccg gcagttggaa gtttttgcag
    15241 aagtattgaa aagtggatca accacccagg cgtcggtgat gctggcgttg tcgcaatcag
    15301 cagtgagcgc agccttgact gacctggaag ggcagcttgg cgttcaactg tttgatcgcg
    15361 tggggaaaag actggttgtt aatgaacacg ggcggctgct ctatccgcga gcgttggcac
    15421 tgcttgaaca ggcggttgaa atcgaacaac tgtttcgcga agacaacggc gcgattcgta
    15481 tctatgccag tagtaccatc ggtaactaca ttctgcctgc agttatcgcc cgttatcgcc
    15541 atgattatcc gcagttgccg attgaactta gcgttgggaa cagccaggac gtgatgcaag
    15601 cggtgctgga tttccgcgtt gatattggct ttattgaagg accgtgccac agcactgaaa
    15661 tcatttctga accgtggctg gaagacgagc tggtggtttt cgccgcgccg acttcgccgt
    15721 tggctcgtgg tccggtcacc ttagaacagc tggccgctgc gccgtggatc ctgcgtgaac
    15781 gcggttccgg cacgcgggat attgtcgatt atctgttgct gtcacattta ccgaagtttg
    15841 agatggcgat ggaattaggt aactccgagg caatcaaaca tgcggtgcgt catgggttgg
    15901 gaattagttg cctgtcgcga cgtgtgattg aagatcaatt gcaggcaggc acattaagtg
    15961 aagttgcggt ccctctgccg cgcctgatgc gtacgttgtg gcgtatacat catcggcaaa
    16021 aacacctttc caacgcgcta cggcgctttc tggactattg cgatcccgca aatgtgccgc
    16081 gttaagttgc tgtacaagaa catgctggtg ctgtgtcgat ttcgtgacgc agcgccttca
    16141 gcatgcattc gccagaaaag agattggctg ctttacttat aatccctggg cgatcatgaa
    16201 ggtgtcttat aaccgtgtat ttctgccgga aggattgcca atcgtctgct acaatcgcgc
    16261 catcattttt aagatggata gcatttttgt atgggttccg aaactaaaac tacagaagcg
    16321 ccgggcttac gccgtgaatt aaaggcgcgt cacctgacga tgattgccat tggcggttcc
    16381 atcggtacag gtctttttgt tgcctctggc gcaacgattt ctcaggcagg tccgggcggg
    16441 gcattgctct cgtatatgct gattggcctg atggtttact tcctgatgac cagtctcggt
    16501 gaattggctg catatatgcc ggtttccggt tcgtttgcca cttacggtca gaactatgtt
    16561 gaagaaggct ttggcttcgc gctgggctgg aactactggt acaactgggc ggtgactatt
    16621 gccgttgacc tggttgcagc acagctggtc atgagctggt ggttcccgga tacaccgggc
    16681 tggatctgga gtgcgttgtt cctcggcgtt atcttcctgt tgaactacat ctctgttcgc
    16741 ggctttggtg aagcggaata ctggttctca ctgatcaaag tcacgacagt tattgtcttt
    16801 atcatcgttg gcgtgctgat gattatcggt atcttcaaag gcgcgcagcc tgcgggctgg
    16861 agcaactgga caatcggcga agcgccgttt gctggtggtt ttgcggcgat gatcggcgta
    16921 gctatgattg tcggcttctc tttccaggga accgagctga tcggtattgc tgcaggcgag
    16981 tccgaagatc cggcgaaaaa cattccacgc gcggtacgtc aggtattctg gcgaatcctg
    17041 ttgttctatg tgttcgcgat cctgattatc agcctgatta ttccgtatac cgatccgagc
    17101 ctgctgcgta acgatgttaa agacatcagc gtcagtccgt tcaccctggt gttccagcac
    17161 gcgggtctgc tctctgcggc ggcggtgatg aacgcagtta ttctgacggc ggtgctgtca
    17221 gcaggtaact ccggtatgta tgcgtctact cgtatgctgt acaccctggc gtgtgacggt
    17281 aaagcgccgc gcattttcgc taaattgtcg cgtggtggcg tgccgcgtaa tgcgctgtat
    17341 gcgacgacgg tgattgccgg tttgtgcttc ctgacctcca tgtttggcaa ccagacggta
    17401 tacctgtggc tgctgaacac ctccgggatg acgggtttta tcgcctggct ggggattgcc
    17461 attagccact atcgcttccg tcgcggttac gtattgcagg gacacgacat taacgatctg
    17521 ccgtaccgtt caggtttctt cccactgggg ccgatcttcg cattcattct gtgtctgatt
    17581 atcactttgg gccagaacta cgaagcgttc ctgaaagata ccattgactg gggcggcgta
    17641 gcggcaacgt atattggtat cccgctgttc ctgattattt ggttcggcta caagctgatt
    17701 aaaggaactc acttcgtacg ctacagcgaa atgaagttcc cgcagaacga taagaaataa
    17761 gtttcctccc ttccttgcta agccctctca accgagaggg ctttttcaat tccatttccc
    17821 tgacaaatca tgcggatata aaatttaaca tttggattga taattgttat cgtttgcatt
    17881 atcgttacga cgcaatcaaa aaaggctgac aaatcagagg ctgttccggc tttctgggat
    17941 gatcacctgc atacaaataa gtccaccgcg atgctgccgt acgcaagggg acgtaaagaa
    18001 gatgtgagcg atacccattt tattttcgta gttacctcat ggagatatgg aatgtttagg
    18061 ttgaaccctt tcgtacgggt cgggctgtgt ttgtccgcta tttcttgtgc atggcctgtg
    18121 ttagcggtcg atgatgatgg cgaaacgatg gttgtcactg catcttccgt ggaacaaaat
    18181 cttaaagatg cacccgccag tatcagcgtc attacccagg aagacctgca gcgaaaaccg
    18241 gtacagaatc tgaaggatgt cctcaaagaa gtgcctggcg tacaactgac gaacgaaggg
    18301 gataaccgta agggcgttag tattcgtggt ctggacagca gctacaccct gattcttgtc
    18361 gacggtaaac gcgttaactc ccgcaatgcc gtcttccgcc acaatgattt cgatctgaac
    18421 tggatcccgg tcgattccat cgaacgtatt gaagtggttc gtggcccgat gtcgtcgcta
    18481 tacggctccg atgcgctcgg cggtgtagtg aatatcatca ccaaaaaaat cggtcagaaa
    18541 tggtcggata ccgttaccgt cgataccacc attcaggaac atcgcgatcg cggtgatacc
    18601 tataacggtc agttctttac cagtggacca ttaattgatg gtgtgctggg aatgaaagct
    18661 tacggcagcc tggcaaaacg tgaaaaggat gacccgcaaa actcaacgac caccgatacc
    18721 ggagaaacgc cgcgtattga aggattctcc agccgcgacg gcaatgtcga atttgcctgg
    18781 acaccgaatc aaaatcacga ttttactgcc ggatacggtt tcgaccgtca ggatcgtgat
    18841 tccgattcgc tggacaaaaa ccgcctggaa cgccagaact actgcgtcag ccataatggg
    18901 cgttgggatt acggcaccag cgaactgaaa tcctacggtg agaaagtcga gaacaaaaac
    18961 cctggcaaca gcagcccgat aacttccgaa agcaatacgg tcgacggcaa atacacgttg
    19021 ccgctgacgg cgattaatca gtttctcacg gttggcggtg aatggcgtca cgacaaactt
    19081 agcgatgcgg tgaacctgac cgggggaacc agctccaaaa cgtctgccag ccagtacgcg
    19141 ctgtttgtgg aagatgaatg gcggatcttc gagccgctgg cgctgacgac cggcgtgcgt
    19201 atggacgatc acgaaaccta cggtgaacac tggagtccgc gtgcctacat ggtttataac
    19261 gccaccgaca ccgtaacggt gaaagggggc tgggcgacgg catttaaagc accttctctg
    19321 ttgcaactta gccctgactg gacgagcaat tcctgccgtg gcgcatgtaa gattgtgggt
    19381 agcccggatc tgaaaccaga aaccagcgaa agttgggagc tggggcttta ctacatgggt
    19441 gaagaaggct ggctggaagg gggtgaatcc agcgttatcg ttttccgtaa cgatgtgaaa
    19501 gatcgtatca gcatcagccg tacgtctgac gtcaacgctg caccgggcta ccaaaacttt
    19561 gttggttttg agacgggcgc taacggacgg cgcataccgg tatttagcta ctacagcgtt
    19621 aacaaagctc gtattcaggg cgtggaaacc gaactgaaaa ttccgttcaa cgatgaatgg
    19681 aaactgtcga tcaactacac ctacaacgat ggtcgtgatg tcagcaacgg cgaaaacaaa
    19741 ccgctatccg atctgccgtt ccatactgct aacggtacgc tggactggaa accgctggcg
    19801 ctggaagact ggtcattcta tgtttctggt cactataccg ggcagaaacg cgccgacagc
    19861 gcgacggcta aaacaccggg cggttatacc atctggaata ccggcgcggc ctggcaggtg
    19921 actaaagacg tcaaactgcg cgcaggcgtg ctgaaccttg gcgacaagga tctcagtcgt
    19981 gacgactaca gctataacga agacggacgt cgttacttta tggcagtgga ttatcgcttc
    20041 tgatgagaag atgcccggcg aaccgggcgg actttcactt cagtaaatac tgcgcatgga
    20101 agcgcaggtg atcctctata aaagaggcga tgaagtagta actgtgatca tatcccggct
    20161 gaatacgcag cgtcatcggc cacgctttct ggcgcgcggc ttctgccaat accgcaggtt
    20221 gcaactggtc ggcaagaaac tgatcattat cgccctgatc gataagcgtc gggatcgcat
    20281 cctgcgcgtt actggcatac atcagcgcgc aactatccca ttccagcatg catttttgtc
    20341 ctcacctaaa taggtgctaa acgctttgat tccccacggg acgctgcacg gattcacaat
    20401 gggcgcaaag gccgaaacgc tggtgtattt acccggattt ttcagcgcca taatcagcgc
    20461 accgtgacca cccattgagt gaccgctaat ggcgcagcgg tcgctgacat taaattgcga
    20521 ctgaaccagc gccggtaatt catcgcgcag ataatcatac atccggtaat gcgtcgccca
    20581 cggcggttgc gtggcattaa gataaaagcc tgcgccctgg cctaaatcgt agccatcgtc
    20641 gttggcaacc ttttcgccgc gcgggctggt gtctggcatc accagtacaa tccccagttc
    20701 cgccgctacc cgctgggcac ccgccttggt ggtgaagttc tcgtcattgc aggttaatcc
    20761 ggaaagccag tacagcactg gtggcggggt gtgatcacga gggggtggga gaaagatact
    20821 gaacgtcatc gggcagttta aggtactgga gtcgtgtcgc cagcgttgct gccagccttc
    20881 aaaacagcgg tgctcttcga gcatttccat gcgtggctcc ttgttgtgtt gtttgcaatt
    20941 tcttcatcat accgtttatt caatgtgctg tgagtaactt tcacttccgt atttgcataa
    21001 cgatgtttta acatctgctg atgaaaggca gcggcaatta caataattat cgctgtgaat
    21061 actggattat gtgcgccgcc tcacgcacaa taatcaagct gtaaatcagc ttaataactt
    21121 tgcccccacg cagggcggag gcgtcacacc tgcaggagaa atcataaatg ccatcactca
    21181 gtaaagaagc ggccctggtt catgaagcgt tagttgcgag aggactggaa acaccgctgc
    21241 gcccgcccgt gcatgaaatg gataacgaaa cgcgcaaaag ccttattgct ggtcatatga
    21301 ccgaaatcat gcagctgctg aatctcgacc tggctgatga cagtttgatg gaaacgccgc
    21361 atcgcatcgc taaaatgtat gtcgatgaaa ttttctccgg tctggattac gccaatttcc
    21421 cgaaaatcac cctcattgaa aacaaaatga aggtcgatga aatggtcacc gtgcgcgata
    21481 tcactctgac cagtacctgt gaacaccatt ttgttaccat cgatggcaaa gcgacggtgg
    21541 cttatatccc gaaagattcg gtgatcggtc tgtcaaaaat taaccgtatc gtgcagttct
    21601 ttgcccagcg tccgcaggtg caggaacgtc tgacgcagca aattcttatt gcgctacaaa
    21661 cgctgctggg caccaataac gtggctgtct cgatcgacgc ggtgcattac tgcgtgaagg
    21721 cgcgtggcat ccgcgatgca accagtgcca cgacaacgac ctctcttggt ggattgttca
    21781 aatccagtca gaatacgcgc cacgagtttc tgcgcgctgt gcgccatcac aactgattaa
    21841 aaggcaggaa ccatggagcg caacgtcacg ctcgattttg ttcgcggcgt cgccattctg
    21901 gggatcctgc tattaaacat cagcgccttt gggctaccaa aggcggctta tctcaatccc
    21961 gcctggtacg atgctattac gccgcaggat gcatggacct gggcatttct cgatctcatc
    22021 ggccaggtga aattcctcac gctttttgcg ctgctgtttg gtgctggcct gcaaatgttg
    22081 ctgccccgtg gcagactttg gatccagtcg cggttaacgc tgttagtctt gttgggcttt
    22141 attcacggtt tattgttctg ggacggcgat attctgctgg cttacgggct ggtggggctt
    22201 ggcgttttgc tgttattggg gctgatttcc gacagccaga ccagccgcgc ctggacgccg
    22261 gatgcatcgg ctattttgta tgaaaaatac tggaagcttc acggcggcgt tgaagcgatc
    22321 agtaatcgtg ccgatggtgt tggcaacagt ttactggcac tgggcgcaca gtatggctgg
    22381 caactggcag ggatgatgct cattggtgcg gcgctgatgc gcagcggctg gctgaaaggg
    22441 cagttcagct tacgtcacta tcgtcgtact ggttttgtgc tggtggcgat tggggtgacc
    22501 attaaccttc ctgccatcgc cctgcaatgg cagctggact gggcgtatcg ctggtgtgcc
    22561 ttcttacttc aaatgccgcg ggaactgagt gcgccgtttc aggcgattgg ctatgcgtcg
    22621 ctgttttatg gcttctggcc gcaattgagc cgctttaagc tggtgcttgc gatcgcctgc
    22681 gtcggacgga tggcgctgac caactatcta ttgcaaacgc tgatttgtac cacgcttttt
    22741 taccacctcg gtttgtttat gcattttgac cgcctggagc tgctggcgtt tgttattccg
    22801 gtatggctgg cgaatatcct cttctctgtt atctggctgc gttacttccg ccaggggccg
    22861 gtggaatggc tctggcgtca gttaactttg cgtgctgccg gaccggcaat atctaaaaca
    22921 tcaagataac gatctggatt acattcgtta acaaaacggc tgtaaccgtt tccattgctg
    22981 tgactcgatt cacgaagtcc tgtattcagt gctgacaaaa tagccgccag caagcagtca
    23041 tttactgcaa tctcataaca ggtagtgaat atgatcacca ttcgtgatgt agcgcgtcag
    23101 gctggcgtct ctgtggcaac ggtttcccgg gtgctcaata acagcacgct ggtcagtgcc
    23161 gacacgcgtg aagcagtaat gaaagccgtg agtgagctgg attatcggcc aaacgccaat
    23221 gcccaggcgc tggcaactca ggttagcgac accattggcg tggtggtgat ggacgtttct
    23281 gatgcgtttt tcggcgcgct ggtaaaagcg gtggatctgg tcgcccagca gcatcagaaa
    23341 tacgtgctaa tcggcaatag ctatcatgaa gcggaaaaag agcgtcacgc cattgaggtg
    23401 ttaattcgcc agcgttgtaa tgcgttgatt gttcactcaa aagcattgag tgacgatgaa
    23461 ctggcgcaat ttatggataa cattcccggt atggtgttaa tcaaccgcgt tgtgccgggg
    23521 tacgcccatc gttgcgtttg cctggataat ctcagcggtg cccgaatggc gacgcgcatg
    23581 ttgctgaata acggtcatca acgtattggt tatctttctt ccagccacgg cattgaagat
    23641 gacgccatgc gtaaagcagg ctggatgagt gcgttgaaag agcaggatat tattccgccg
    23701 gaaagctgga ttggcactgg tacgccggac atgccgggcg gtgaggcggc gatggttgaa
    23761 ctgctggggc gcaatctaca acttaccgct gtatttgctt ataacgacaa tatggccgct
    23821 ggcgcactga cagcattaaa agataatggc attgcgattc cgttacatct ctcaatcatc
    23881 ggtttcgatg atattcccat cgcccgttac accgacccgc aattaacgac cgtgcgttat
    23941 cccattgctt caatggctaa attagccacc gaactggcct tgcagggggc agcaggcaat
    24001 attgatcctc gtgccagcca ctgttttatg ccgacgttag tgcgtcgcca ttctgtcgca
    24061 acgcgccaga atgcggcggc gatcactaac tcaacaaatc aggcgatgta accgctttca
    24121 atctgtgagt gatttcacag tatcttaaca atgtgatagc tatgattgca ccgttttaat
    24181 gttgtaaccc gtatgtaaca gtgaataatc actttcaccg aggtaatagc gtcataacaa
    24241 caattaaagc cgttttctgg agcgttaccg ggcatggaag aacgaatttt aaaaagtgag
    24301 cttcggcgtt cagtaacact tcattaactc tactgccccg ccgagcattt atctcaagca
    24361 ctaccctgca taagaaaaac cggagatacc atgaataaga aggtgttaac cctgtctgct
    24421 gtgatggcca gcatgttatt cggtgccgct gcacacgctg ctgatactcg cattggtgta
    24481 acaatctata agtacgacga taactttatg tctgtagtgc gcaaggctat tgagcaagat
    24541 gcgaaagccg cgccagatgt tcagctgctg atgaatgatt ctcagaatga ccagtccaag
    24601 cagaacgatc agatcgacgt attgctggcg aaaggggtga aggcactggc aatcaacctg
    24661 gttgacccgg cagctgcggg tacggtgatt gagaaagcgc gtgggcaaaa cgtgccggtg
    24721 gttttcttca acaaagaacc gtctcgtaag gcgctggata gctacgacaa agcctactac
    24781 gttggcactg actccaaaga gtccggcatt attcagggcg atttgattgc taaacactgg
    24841 gcggcgaatc agggttggga tctgaacaaa gacggtcaga ttcagttcgt actgctgaaa
    24901 ggtgaaccgg gccatccgga tgcagaagca cgtaccactt acgtgattaa agaattgaac
    24961 gataaaggca tcaaaactga acagttacag ttagataccg caatgtggga caccgctcag
    25021 gcgaaagata agatggacgc ctggctgtct ggcccgaacg ccaacaaaat cgaagtggtt
    25081 atcgccaaca acgatgcgat ggcaatgggc gcggtagaag cactgaaagc acacaacaag
    25141 tccagcattc cggtgtttgg cgtcgatgcg ctgccagaag cgctggcgct ggtgaaatcc
    25201 ggtgcactgg cgggcacagt actgaacgat gctaacaacc aggcgaaagc gacctttgat
    25261 ctggcgaaaa acctggccga tggcaaaggt gcggctgatg gcaccaactg gaaaatcgac
    25321 aataaagtgg tccgcgtacc ttatgttggc gtagataaag acaacttggc tgaattcagc
    25381 aagaaataag actgattggg cgcagtcgtt tactgcgccc ttattgtatg gccaacaagg
    25441 taaaattata tggtcagctc aacgactccg tcttccgggg aatacttgtt ggaaatgagc
    25501 ggtatcaaca agtcctttcc tggtgttaag gcacttgata acgttaattt aaaagtccgg
    25561 ccacattcta tccatgcatt aatgggggaa aacggtgcag gaaaatcgac attattaaaa
    25621 tgcctgtttg gtatttatca aaaagactcc ggcaccattt tattccaggg taaagagatc
    25681 gatttccatt ccgccaaaga ggcactggaa aatggtattt cgatggtaca ccaggagtta
    25741 aacctggtat tacaacgttc ggtgatggat aacatgtggc tggggcgata tcccaccaaa
    25801 ggcatgtttg tcgatcagga caaaatgtac cgcgaaacca aagcgatttt tgatgaactg
    25861 gatattgata tcgatccgcg tgcgcgcgtc ggcacattat ccgtttcgca aatgcagatg
    25921 atcgaaatcg ccaaagcgtt ttcctataac gcgaaaattg tgattatgga tgaaccgact
    25981 tcttcgttaa ccgaaaaaga ggtcaatcat ctgttcacta ttattcgtaa attaaaagag
    26041 cgcggctgcg gtattgttta tatctcgcat aaaatggaag aaatcttcca gttatgtgat
    26101 gaagttaccg tattgcgcga cggtcagtgg atcgccaccg aaccgctggc aggactgacg
    26161 atggacaaga tcatcgccat gatggttggg cgttctctta atcagcgttt tcctgacaaa
    26221 gaaaacaagc cgggcgaagt catcctcgag gtgcgtaacc taacgtcact gcgccagccg
    26281 tcgattcgcg atgtctcgtt tgatctgcat aaaggggaga tcctcggtat tgccgggctg
    26341 gtgggggcga aacgtaccga tattgttgag acgttatttg gtattcgcga gaaatcggct
    26401 ggcaccatta cgttgcacgg caaacagatc aataaccata atgccaacga agccataaac
    26461 cacggatttg cactggtaac tgaggagcgc cgctcaacgg gaatttatgc ctatctggat
    26521 attggtttta actcgttaat ttccaatatt cgcaactaca aaaataaagt tggtttactg
    26581 gataactcgc ggatgaaaag cgatacccag tgggtgattg attcgatgcg ggtaaaaacg
    26641 ccgggtcatc ggacgcaaat tggttcgctc tccggtggta atcagcaaaa ggtgattatt
    26701 ggtcgctggc tactaacgca accagaaata ttaatgctcg atgaaccgac gcgcggtatt
    26761 gatgtcgggg cgaagtttga aatttatcag ttaattgccg aactggcgaa gaaaggcaag
    26821 gggattatta ttatctcctc tgaaatgcct gagttgttag ggataacaga ccgtattctg
    26881 gtcatgagca atggtctcgt ttccggaatt gtcgatacaa aaacaacaac gcaaaacgaa
    26941 attctgcgtc ttgcgtcttt gcacctttaa gattaggggc ttcccatgag tgcgttaaat
    27001 aagaaaagtt ttcttactta cctgaaagag ggcggtattt acgtcgttct tttagttttg
    27061 ctggcgatta ttattttcca ggacccaaca tttttaagtc tgttgaactt aagtaatatt
    27121 ctcacccagt catcggtgcg tattattatc gcgctcggtg tggcagggtt aattgtcacc
    27181 caggggaccg atctttctgc tggtcgtcag gtagggctgg cggcagtggt ggctgcgaca
    27241 ttattgcagt ccatggataa cgccaacaaa gtgttcccgg aaatggcgac gatgccgatt
    27301 gcgctggtta ttctgattgt ctgtgccatt ggtgcggtga tcggtttgat caacggtctg
    27361 attatcgctt atctcaacgt gacgccgttc attaccacgc tcggcacgat gatcatcgtc
    27421 tatggcatca actcgctcta ttacgacttt gtcggggcgt cgccaatttc tggttttgac
    27481 agtggcttct ctacctttgc tcagggcttt gtcgcgctgg ggagtttccg tctctcttac
    27541 atcaccttct acgcgttgat tgcggtggcg ttcgtctggg tgttgtggaa caaaacccgc
    27601 ttcggtaaga acatttttgc cattggcggt aacccggaag cggcaaaaat atctggtgtc
    27661 aacgtcggcc tgaacctgct gatgatctac gcgttgtctg gcgtgttcta tgcctttggc
    27721 gggatgttag aagccggacg tatcggctct gccaccaaca acctcggctt tatgtatgag
    27781 ctggatgcta tcgcggcgtg cgtggtaggc ggtgtatcgt tcagcggcgg tgtggggacg
    27841 gtgattggcg tggtgaccgg ggtaattatt tttaccgtca tcaactatgg cctgacgtat
    27901 atcggcgtaa acccatactg gcagtacatc atcaaagggg cgattattat cttcgccgta
    27961 gcgctggatt cactgaaata cgcgcgtaag aagtgatgtg attgcggata ttgccggatg
    28021 cgtctctggt tttacaacgt taccgggtgt tctttctcgc ctttcttaaa cttcacttcc
    28081 ccgagctcaa tacaacccac cgggcagacg tgaccacaaa gcagacaacc cacacatttc
    28141 tcggtattac aatgcggtgt gcgggttttc tcgctccatt ccatcgccta gtgaccgccg
    28201 tcgtaacagg aaatataaca gcgtccacag ccaacacatt tgtcaagatt gatacgggga
    28261 tagacaatat aactgcggtc taaatcttcc gccgggacaa tattgttatt cgccagacct
    28321 accatctcct gcaacgaatc aaatccctga tcggcgagat aatgcgataa cccgctcgcc
    28381 atatcttcta catgcgatag ccgtactgca taatgccggt ggtgacctgt aacgttgctg
    28441 cgccgagcaa taaaaactcc gccgcatctt cctaggtttc aatgccgccg ataccgctga
    28501 ttgggaaatc gcgcagttct ggatgggtgc gcatttgctg aatgaagcgc agggcgatcg
    28561 gttttaccgc tttaccggaa tatccggaaa tactcgattt accgttaacg atcggcatac
    28621 cgattttctg attaagatcg atattggtga tggatttaac ggtgttaatt gccgcaatgc
    28681 catctgcgcc gccgcgcttc gccgccagcg ccacttcgca catatcaccg atattcggcg
    28741 tcatcttcgc cagcattggc agcgtggaac cccgtttcac tgcccgacaa tatttttcta
    28801 ccagctccgg gctttgcccg acatcgctac ccatcgcatg agaagtcatt tgcggacagg
    28861 agaagttaca ctcgatcata tccgcgccag ctgctcccat tgctgctcat tttcccccat
    28921 gatcgaagcg atcaatactt tgtccgggta atcttccttc agccgacgca gggcggtcag
    28981 attctcttcc aacggatgtt cagcaatctg ctccatattt ttgaagccga taaaaccggt
    29041 atcttctttc accagatgat caaaacgcgg cgagacttcg ttggcgataa aaaagccgat
    29101 cgttttaaac accacaccgc cccaacctgt gtcgtaggct ttggcacaca tctcatagca
    29161 gttgcctacc ggcgaagaag agaggcagaa cgggttggga aagttcacgc cgcaaaaagt
    29221 aatcgaaaga tctttcgtta acatgagcaa gctccctcta aatagtgatg aatcgcctcg
    29281 gcggcttctt tcccggtttt cacggcatag accacggttt tgccaccctc aacaatatcg
    29341 ccagcagcaa agacttgcgg atcgcgggtc tgataatttt gtgttttgat ggtgttacgc
    29401 tgctgctcta actccgcaaa ggcatccagt ctggcatgct gaccgacggc gagaataatt
    29461 ttatctgccg ccatcgtcag ttcgcctgat aaccgtacat gcttaaacgt gactttattg
    29521 ccttccacgg ctactggcgt gaatccatcg atgatcgaaa cgcccagctc ccgggcgctg
    29581 gcaaactctt tttcgcttgc cggaaactca tctaactctt cacgtgctac gcaagttacc
    29641 gcctgacagc cgagaacttt cagcgtgctg gctacgtcca tcgcgacatc accgccgccg
    29701 ataattaatg cgctttgtgg aatgctgata tcgccttgcg cctgtcgtac acgttgcagg
    29761 aagtcgacgg caatctcaac gtcgctatgc tcaaacagcg gtagcccgga accgcttgat
    29821 aaccccacgg tgaccagtac cgcgcggttt tccgctttca gttgctccag ggtgagtgtt
    29881 ttaccgattt cgttattgca cttaatggtc acgcccattt tttcaatacg ggcgatctct
    29941 gcatccagca ctgattgcgg taaccggaat tgcggaatac cgttacgcaa ccagccaccg
    30001 gggtgcgctt ctttctcata aatcgtgacg tcataaccct ggtttgtcag tgtcacactg
    30061 gcctgcaatc cggcaggacc tgcgccaata atcgcgactt tgccgagcgt tttagtaccg
    30121 ggctgataaa tttccatccc ggtttgttgt tcaaaatcag taacaaaacg ctgtaagcgg
    30181 ccgatatcaa tgggagcatc aacaccggca cgggtacaac cgctttggca taatttctcc
    30241 gtcgggcaca ctctggcaca aacggcaccg agggcattat tttcgcgaat tgtctcggca
    30301 gcgcctttaa aattacgaaa gtagattgag cgaataaatt tccccggatc ggtctgtgcc
    30361 gggcaagcct gactacaggg agcgtcgtga cataataaac agcgagaggc ttctttaatc
    30421 gccagtaacg gcgtaaaagc cggagtgagt tcatccagat aattttgttg cggcatgtga
    30481 tcttccttaa tgctatgagt aattatggcg aacgctaaat aaatattgcg tgctgatatt
    30541 aaggctatgc gcgtggggaa atttaagctg tgaaaggatt cacaaatagc tgcggttcag
    30601 aatgttatat ccgcagctta ttgagtgaag taataattat tctcaataat taacagaaaa
    30661 gagaaaatat taacgataga aaataagata aacaatcatg ccgatagata aaattccgaa
    30721 gccaatatat gccaccatat aagcgaagaa gagtccggga ttttttgctc gccagacata
    30781 caccggttcc ggtgcatttt taacccgttt atcaatcgcg cctgcgcgcc agcctttcca
    30841 tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg gcaacgacaa aaaactgctg
    30901 tacatccata actactttcc ttgtttcttt tgtaattcaa gtaactgttc gggtgtaacg
    30961 gcgggatacc cctgcgcatc ttccggcacc tggtgcatag ccggaatagg gaccagcggc
    31021 cctaaaaaac gcggttcacg tttaaaaata taaaggtcag ccagcgcacc gaaacgggcg
    31081 gcaaattcgc gaatacgtac tgacagcata tctttcggtg acggtacggc ataacactga
    31141 gcctgaatcc ccatatgcag cgcaataaat aatgctcgct cacagtggaa acgttgggtg
    31201 ataataatga aatcattagt atcgaaaact ttgcgtgtac gcacgatgga atccagcgta
    31261 cgaaagcctg cgtaatcgag aacaatatct gatgggtcga caccagcagc gattaaatct
    31321 ttgcgcatgg tcatcggctc gttataactt tgcaatgcgt tatcgccgct cagtaataga
    31381 taatttacct taccgctgtt ataggcatta atcgctcctt gaatgcggta gcgataatac
    31441 tgattaataa cgccggtacg ataatatttt gctgtgccga gcaccacgcc gacctggcgg
    31501 taggggagat cctgcaattc gtcgtagata taaggcgcgg ttttccagct catccagcga
    31561 tcgaggccga gcacagtcaa cagcagcaag ccgatcagga ctaacaggct gaggaacacg
    31621 cgctttaaca tgcaggagac tcgagctaag gaaaatttcc atcaggctac tttacccgct
    31681 ggttttgcgc aagaaacagc aaactgtttg ccgggaattt cgacaccagg agagggctgg
    31741 caagccggac aaggcgttca cgccgcatcc ggcaccaggc ttaagcgaga agcacgcggt
    31801 cgatactgtg acagccgaga gctttcagcg ttgcggaggt ggcatcccac tgaatcaacg
    31861 gcgcatcggc tttttctgcc agaaccgcgc gctggatatc cgggtaatca taacccttga
    31921 gattcaacag aattaacgcc ccctgcaacg gcggcagagt cgggttgaat gcggcgtttt
    31981 cagcgtagct gccgctgaaa atacgaccgt ctttacattc cagcgcgaca ccgcttggtg
    32041 acttactgta aggcatgtgc gaacggtttg ccgctgcaat cgccgcctga gaaagcgcat
    32101 caccagtcag cgcatagccg tgatcctgct cgtccatcag cagcgtttta atctccagat
    32161 ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc gtgtgcctcg cggcccggca
    32221 aatgaatacg cagatccaga ccgctgttca gttcattcat aaactgacgg cagtgaccac
    32281 aaggcgtata gttaacggtg atggctgcaa gcgctttttc accactcaac caagcgtggc
    32341 tgattgcgct ttgttcggca tgaacggttt gctgcattgt tgcaccaata aattccatat
    32401 tggcaccaaa ataccaggtt ccgctcacac cgcgcgcaat tgcgccaaca ttaaaattcg
    32461 acaatggcgt acgcgcacag gccgccgcca gcggaagtag tgcgaatgcc agcgcgtctt
    32521 cgtccagtcc cgttgcgctc ttcagcgatg agacttgctc cccggtcaac aaagcgggga
    32581 agtacttgtc tgccagaata ggttccagtg cagattgcaa gttatccgca agttgggcaa
    32641 aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa tcatgtaatg ggataacagt
    32701 ttacgaaccc gttatggctt tatatgtgat ctgaatctca ttaattatgc aacacgtgaa
    32761 acttacatca aaaatgcgcg acgtatcgca aattttagcc cattactgcc agaataatcg
    32821 ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca taacaccagc gctagcgaac
    32881 taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc cgtaccgagg gcgtgcgagg
    32941 cagtccccat tgccagaccg cgcgcagctt tggtacgaat acgcatcgca ttaagcaatg
    33001 tatggccaaa taccgcgccg aggatgccga cgaaaatcac gcaaactgcg ctgattgccg
    33061 gaataccgcc aatactgccg ccaaccgcca ttgcaatagg cgtggtgact gatttcggca
    33121 ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa tgccacggaa gtccccgtca
    33181 ccatcgcaac cacgctgccg ataaaacaga tggtgatgat cgatttccag cgcgcgcgga
    33241 tctggtgtag ctgctcatat aaaggatagg ccagcgcgac gaccgccggt tgcagcagat
    33301 cgtttagcac ttcgctacct ttaaagtaac tgtcgtaaga gatgccggtc agcattaaaa
    33361 aaggaatgat caccaccatt gctaccagca acgggttaag caggggaaac ttataccgtg
    33421 ccgccagttt gcgggcggca aaaaagacaa tcaacgtcag cggtaatgac caccagatat
    33481 tcgccatcat tcttctgatc ctttctgacc tacgacttta cgttcaccgt gtaccagttg
    33541 tgaactccag ctcaccacca gaaaaaccac cagcgtactg acagcacagg aaaccactac
    33601 cgggccaaac tgcgcgcgga gcaaatcaaa atattgcatg acgcctacgc caatcggcac
    33661 aaacaatagc gccatatagc gaatcagtac gtagcacccc ggattgaccc atttcgccgg
    33721 aagaatttgc aaggccagca ggacaaacag gatctgcatc ccgatgatgc tgcccggaat
    33781 ggttaccggt agcagggaag caatgaaaat gcctgcatac aggcaggcat aaatcaggat
    33841 gaaagcgcgt aaatattgcc agataatgtt cagtgtctta ctcataatca acaccccaga
    33901 tgagatggat tcatcataca attaattcgt aaaacgtgct accgatcaca ttaagaaatc
    33961 agcgcttatt ttttcgctac ccggtattac gcaatgagca gcgaactcag gcatacatca
    34021 agcatagaga gggattgcag tttgcagcaa tccctctttg cagaccttta atccgctgac
    34081 cataggcgct aacatagcgc ctcatttttt tgcgggtgat gatatgcgtg tgttactggc
    34141 accgatggag ggagtgcttg actctctggt gcgtgaattg ctgaccgaag ttaacgacta
    34201 cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa ctgctgccgg taaaagtctt
    34261 tcatcgcatt tgccctgagc tacaaaacgc cagccggaca ccatctggta cgctggtgcg
    34321 cgtgcagttg ttaggtcagt tcccacaatg gctggcagag aacgccgccc gtgcggtgga
    34381 gttaggtttc tggggcgtgg atctcaattg cggctgcccg tcgaaaacgg ttaacggtag
    34441 cggcggcggg gcgacgttac tcaaagatcc tgaactcatc taccaggggg caaaagcgat
    34501 gcgtgaagct gtaccggcgc atttgcctgt cagcgtgaaa gtgcgtctgg gctgggacag
    34561 cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag gctggcgcta cggagctggt
    34621 ggtgcatggg cggacgaaag agcagggtta ccgcgcggag catattgact ggcaggcgat
    34681 tggcgagatt cgccagcggc tgaatattcc ggtgattgcc aacggtgaaa tctgggactg
    34741 gcagagcgcg caacaatgca tggcgatcag cggctgcgac gcagtgatga ttggtcgcgg
    34801 ggcgctcaat attcccaacc tgagccgggt ggtaaaatat aacgaaccgc gaatgccgtg
    34861 gccggaggtg gttgctttgc tgcaaaaata tacccgtctg gaaaagcagg gcgataccgg
    34921 gttatatcac gttgcgcgga ttaaacagtg gttgagttat ttgcgtaaag aatacgatga
    34981 agcaacggaa ttatttcagc atgttcgggt gttgaataac tcccctgata ttgcaagggc
    35041 tattcaggca attgatatcg agaaactcta aaaaccatag tgatgttttt gacaatgtgt
    35101 tagctcttta tcaataggcc gcacggttgt ttggtggcgt caaaatcgcc gaggcgttcc
    35161 ctgaaggccg gggcagccca catggatgtg ggctgagggc gcgttttaca gggatgttac
    35221 ctcgcgcccg acccggtagc cgtaagggat aagccgaggg caccgcgcag cggcgatttt
    35281 gttcgccaga gcccgggggg gcagggggcg gcggcgattg gtcgccccct gcgcgctcct
    35341 tgcgccagtg attatatgaa gcaatgcaca tgacaggagc gcaacaggat gagagactga
    35401 aatattctcg ttattttcgg atctgatgac attaattaat tttaaaatat catcttaaat
    35461 acgccagtca ccaccagtag cccaatcagg aaaataatca atacagccca gagtataatt
    35521 ttcattggcg ttcctttatt tgtgagtgct gaataagtat aggaaaaaac gaagtccttt
    35581 gccgttatcc ataaacgatt aagataaatc ggaagatatc atttacttag tctatatttt
    35641 tctgggtatg tcgttttatt gctactgatg tattgccacg cgaaactaaa aaatatatcg
    35701 caacacacgg taatctccgc gcaccgtttc ttacctgatt attcccccat gaatcgtgat
    35761 tccttttatc cagccatcgc ctgttttccg ctgttactga tgctggccgg gtgtgcgcct
    35821 atgcatgaaa cccgccaggc gttaagccag caaacgcccg ctgcacaagt tgacaccgca
    35881 ttacccacgg cgctgaaaaa tggttggcca gacagccaat ggtggctgga gtatcacgat
    35941 aatcagctca cttcattaat taacaatgca ctacagaacg caccggatat gcaggtggct
    36001 gaacagcgca ttcagctagc tgaagcccag gcgaaggcag ttgccactca ggatggtccg
    36061 cagatcgact tttcggcgga tatggagcgg caaaaaatgt cggcagaagg gctaatgggg
    36121 ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc cgtggtacac caacggaact
    36181 tttggcttaa cggcgggctg gcatctcgat atctggggaa agaatcgggc ggaggttact
    36241 gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg agcaaacccg ccaattgctg
    36301 gctggcagcg tagcccgcct gtactgggag tggcaaaccc aggcggcgtt aaacacggtc
    36361 ttgcagcaaa tagaaaaaga gcagaacacc attatcgcga ccgatcgcca gctatatcag
    36421 aacgggatta cttcttcagt tgacggtgtg gaaaccgata ttaatgccag caaaacccgg
    36481 cagcagctca acgatgtcgc ggggaaaatg aaaattattg aggcacggtt aaatgcactc
    36541 acaaatcacc agactaagtc attgaagctt aaaccggtcg cgttgccgaa agtggcaagc
    36601 cagcttcctg atgaactggg gtactcctta ctggcccggc gggcagattt gcaggcggcg
    36661 cactggtacg ttgagtcatc gttaagcact attgatgcgg caaaagcggc attttatcct
    36721 gacatcaacc tgatggcctt cctgcaacag gatgcgttgc acttaagcga tctgttccgt
    36781 cattccgcgc agcaaatggg cgttacggca ggcctgacgc tacctatttt cgatagtggt
    36841 cgtcttaacg ccaatctcga tatcgcaaaa gccgaaagca acttgtctat cgccagctac
    36901 aacaaagcgg tggttgaagc agtgaatgac gtggcgcggg cagccagtca ggttcagaca
    36961 ctggcggaga aaaaccagca tcaggcgcaa attgagcgcg atgccttgcg tgtggtaggt
    37021 cttgcgcagg cccgctttaa cgcgggcatc attgctggtt cccgcgtcag cgaagccaga
    37081 atcccggcgc tgcgtgagcg ggccaatggc ctgttattgc aagggcagtg gctggatgcc
    37141 tccattcgac tcaccggtgc gctgggcggg ggttacaaac gctgatgata tgcgccttct
    37201 atacttaacg tttattcagc gttaagtgga gaactcgatg gcataggtcg cgataattac
    37261 cgcttccgat tcggggatcg gcaaagagtg cgcgttatta ctggcgcagc aggggtttga
    37321 tattggcatt acctggcact cagatgaaga aggggcaaaa gataccgcgc gtgaggtggt
    37381 tacccacggc gtacgtgcgg agatcgtgca actggatctc ggcaatctgc cagaaggtgc
    37441 gcaggcgctg gagacactca tccagcgatt tgggcgtatt gatgtgctgg tgaataatgc
    37501 gggtgcaatg accaaagcgc cgtttcttga tatggctttt gatgagtggc gcaagatttt
    37561 taccgttgat gttgatggtg catttttatg ctcgcaaatt gcggctcgtc agatggtgaa
    37621 ataagggcag ggcggtcgca tcatcaacat tacgtcggta catgaacata cgccgctgcc
    37681 ggatgccagc gcctacacag ccgctaaaca tgcgctcggc gggttaacca aaacgatggc
    37741 gctggagttg gtcaggcata agattttggt gaacgcagtc gcgcctgggg cgatagccac
    37801 gccaatgaac agcatggatg atagcgacgt gaagcccgac gcggagcctt cgattcctct
    37861 acggcgtttt ggcgcaacgc atgagattgc cagcctggtg gtgtggcttt gttcggaggg
    37921 cgcaaattac acgacagggc agtcgttgat agtggatggc ggctttatgt tggcgaatcc
    37981 gcagttcaac ccagaatagc gcagtgaggt gcgtggatat ttcaatgggt taggccagat
    38041 aagacaccag cgtcgcatca ggcatggaac acccggtgcc ggatgtggag tgaatgctta
    38101 tctgtggcaa ctgctttacg cctgattatc cggctttttc ttcccgcgcc gtttcagcca
    38161 ccagcgcacg cccaccacca gaaccacgac cagaatcaac cagacccagt gcttcaaatg
    38221 ctggtcgaga ttgtgcaacc acggcgcaat cacctgacca ccagcgtaac caatagtggt
    38281 aaaaatcaac gcccaggcaa atgcgccgag aatattcagc ggcagaaaga ttttcggcgg
    38341 cagctggctg gcaccaatca gcgtcgggcc aatcacccga aagccataca taaagcgcgt
    38401 accaatgaca aacagatacg gatggcgttg gataagtttc tgtgcccgct caattttatc
    38461 ctgatgtttc gagaaacggc gtaacagctt gccgccaaac cgccgcccgc acagatagag
    38521 cacctggtcg ccaatcatgc caccaagcgc cacagaaagt accaccagtg ggaactttaa
    38581 tagcccctga tgtgccgcaa cgcctcccag caaagtcacg gtttcacctt ccgccaggct
    38641 accgataacc agcgcggcat aaccatattg tgagatgagt gtattgagat ccataggcca
    38701 gtttgtctcc ctttggttgc ctgtaagcat acaccctgtg aatcattcac gctgaaaaag
    38761 gacatacatc agacggtgca aattacatgg atgaacaaaa aataatcaca agctggctta
    38821 tacttgaact gtcaggttca tgaactcaca acaagggggc ggtatgagcc atgtctgggg
    38881 actgttttcc catcccgatc gtgaaatgca ggtgattaat cgcgaaaacg aaacgatttc
    38941 tcaccattac acccaccacg ttttgctgat ggcggcgatt ccggtgattt gcgccttcat
    39001 tggcactaca cagattggct ggaattttgg cgatggcact atcctgaagt tatcctggtt
    39061 taccggactg gcgctggctg tcttgtttta cggcgttatg ctggctgggg ttgcggtgat
    39121 ggggcgggtc atctggtgga tggcgcgcaa ttacccgcag cgtccgtctc ttgcgcactg
    39181 catggtcttc gcgggctacg ttgcaactcc gctgttttta agcggtctgg tggcgcttta
    39241 tccactggtc tggttgtgtg cgctggtcgg cacggtggcg ctgttttaca ccggctacct
    39301 gctttacctt ggtattccga gcttcctgaa tatcaacaaa gaggaaggcc ttagcttctc
    39361 cagttcgacg ctggcgattg gcgtgctggt actggaagtg ctgctcaccc taaccgttat
    39421 actatggggc tacggttatc gtttgttcta gaaagttctt tgacgttgca ttgctggcgt
    39481 aaatgtcagc aatgcagcat ttactcacga ttctcctttg acgatcgcac tttttgctcg
    39541 ttatgatggc gtcgctagcc tcagtaaatc cttactccgg cggtgcgcaa cccgtgcgcg
    39601 tgaaccacta tctgaatgct catcatgccg aaatttcgag tttctttatt tagcttggcc
    39661 ctgatgctgg ctgtgccttt tgcaccgcag gccgttgcga aaacggcagc cgctaccacc
    39721 gcttcacaac cggaaattgc ctccggtagc gcgatgattg ttgatctgaa taccaacaaa
    39781 gtgatctatt cgaaccaccc ggatctggtg cgtccgattg cgtctatcag caaattaatg
    39841 accgcgatgg ttgtgctgga tgcacgactg ccgctggatg aaaaactaaa agtggatatc
    39901 agccagacgc cggagatgaa aggggtctat tcgcgcgtac gactgaatag cgaaatcagc
    39961 cgtaaagata tgctgttgct ggcgctgatg tcttcagaaa accgcgcggc ggcaagcctt
    40021 gcgcaccatt atcccggtgg ttacaaagcc tttattaagg caatgaatgc gaaagcgaaa
    40081 tcgctcggaa tgaacaacac gcgctttgtt gaacctaccg gattgtcggt gcataacgtt
    40141 tcaactgccc gtgacttaac caaactgctc attgccagca aacaatatcc gttgatcggg
    40201 cagttaagta ctacccggga agatatggca accttctcta acccgacgta tacgctgccg
    40261 ttccgcaata ctaatcatct ggtgtatcgc gataactgga atattcagtt aaccaaaacc
    40321 ggctttacca atgcggcggg ccattgtctg gtgatgcgta cggttatcaa taataaaccg
    40381 gtggcgctgg tagtgatgga cgcgtttggc aaatataccc attttgccga tgccagccgc
    40441 ctgcgtacct ggattgaaac cggtaaagtg atgcctgtgc cggcagcagc gttgagctat
    40501 aaaaaacaaa aagccgccca aatggcggcg gcggggcaga cggcacagaa cgattaagtg
    40561 aattcggatg gcgatactct gccatccgta atttttactc cacttcctgc cagtttttcc
    40621 gtttactggt tttaccgatc cccggattca tgctattggt cggatcattc tcgcgataga
    40681 acttctgcaa cgtctccggt gctttataca aatgaccgac gttatgctcg gcagggtact
    40741 gcgcgccgcg ctgctgtagc agttccagca tctgttcttt taacgcatgc acatccacgc
    40801 cttttttcac tatgtaatcc tgatggaaga catagcacat aaaatggccg taatagagct
    40861 tgtgcaccag ctggctgtcg atctccggcg gtaaatgctc ataccactcg gtgtcgttac
    40921 gccgcagagc gatatccagt gccagaatgt cttcgacttc atcggaatgc accgcctgat
    40981 aacgaattgc tgcgcccgca gcggcgaaac ggtgtaaaaa cgctttgcta ccttcctccg
    41041 gcgtacagac aaagaaatcg ccctcggcct gtttgaaata atccaccagc cacgatttgg
    41101 cttcaccgac gccatcgcct gccattttta acagcagatg atgctcgtat ttatcgcgcc
    41161 agtttttcat gcgcggcggt aaatggctgg ggaacaggtg accgaatttt tgcattgcac
    41221 ggtcagtaaa atgcggacgg aagaatttca ctttctccag catcgcatcg gtgcgtccct
    41281 tgagattaaa gaagaacggc atcttgtcgg tgcctaactt atcaatcatc aggaaggtgt
    41341 ctttgccgta tttttccgca atatcgtaga tatcccggtg catatattcc ccggcaaccg
    41401 gcagattttc gaagttagcc agaatatgac ggcggatttc ggtcagcact tccggctggt
    41461 tggtgccgat ataaaacacc tgctgatttt tttccgcttc gaaggtatca agacgtaccg
    41521 caaagacggc cagcttcccg gcgcaaccag aagattcaaa taaccgatcc ggatcggcgt
    41581 tataacgtgc gggcgtgtcg gcttcaatat cacgaacgcg gtggacatag tcataatcgt
    41641 gggcgtgacg accatcgtga cgcacatcgt catctttgat gcgatcgtca tccagcttgc
    41701 taaggatctg ctccggcgtt tcgcccagat caatccccag atggttcacc agcgtcagtt
    41761 tgccatcttc atctatacgc gcgaataacg acatttcggt atacgccggg ccacgttgca
    41821 ccagcgagcc gccggagttg ttacaaatac cgccgatgac cgatgcgcct atacacgatg
    41881 atccaatcac cgaatgcggt tcgcgtccca gcggtttgag ggctttttcc agcgaataga
    41941 gcgtggtgcc cggataggcc agcacctgtt cgcccttgcc gagaacgtgc agcttgtcga
    42001 gacgcagggt gctgatgatc acgatatcgc gatcatagtc gttaccgttt ggcgtcgatc
    42061 cttcggtcag gcctgtattg gcagcctgca tcagaataat tttgtcggcg gtgacgcagg
    42121 ctttcagcac ccgccacaat tccagtagtg agccagggaa aacgacagcc agcgcgtcgc
    42181 cctgaccaga acggaagccc ttgcgatagc gggccgtttt tgcgggatcg gtgagcaggt
    42241 gtgaatgacc gaccagacgg gcaagttcat tcaaaaaggc tttattatca gttgttgtca
    42301 tggaagacat tttccactcc ttgtggtggc gaaaaaatat cacaaactag aatagcgcct
    42361 tgtatgacaa ttcagcgagt gaatattttg aaaattcaga caataaactg agcattcttt
    42421 ttctctctcc atcatgctta tggcaaacta cggtttttgc accattttcg ctgccacgtc
    42481 gggcaacaaa ggaagaaaaa tccatatgaa atggctatgt tcagtaggaa tcgcggtgag
    42541 tctggtcctg cagccagcac tggcggatga tttattcggt aaccatccat taacgcctga
    42601 agcgcgggat gcgttcgtca ccgatctgct caagaaaatg acggttgatg agaaaattgg
    42661 tcagctgcgc ttaatcagcg taggcccgga taatccgaaa gaggcgatcc gcgagatgat
    42721 caaagacggt caggttgggg cgattttcaa caccgtaacc cgtcaggata tccgcgccat
    42781 gcaggatcag gtgatggaat taagccgcct gaaaattcct cttttctttg cttacgacgt
    42841 gctgcacggt cagcgcacgg tgttcccgat tagcctcggt ctggcctcgt cttttaacct
    42901 cgatgcggtg aaaacggtcg ggcgtgtctc tgcttatgaa gcggcagatg atggcctgaa
    42961 tatgacctgg gcaccgatgg tcgatgtctc gcgcgatccg cgctggggac gtgcttccga
    43021 aggttttggc gaagatacgt atctcacctc aataatgggt aaaaccatgg tggaagcgat
    43081 gcagggtaaa agcccggcag atcgttactc ggtgatgacc agcgtcaaac actttgccgc
    43141 atacggcgcg gtagaaggcg gtaaagagta caacaccgtc gatatgagtc cgcagcgcct
    43201 gtttaatgat tatatgccgc cgtacaaagc ggggctggac gcaggcagcg gcgcggtgat
    43261 ggtggcgctg aactcgctga acggcacgcc agccacctcc gattcctggc tgctgaaaga
    43321 tgttctgcgc gaccagtggg gctttaaagg catcaccgtt tccgatcacg gtgcaatcaa
    43381 agagctgatt aaacatggca cggcggcaga cccggaagat gcggtgcgcg tggcgctgaa
    43441 atccggaatc aacatgagta tgagcgacga gtactactcg aagtatctgc ctgggttgat
    43501 taaatccggc aaagtgacca tggcagagct ggacgatgct gcccgccatg tactgaacgt
    43561 taaatatgat atggggttgt ttaacgaccc atacagccat ttggggccga aagagtctga
    43621 cccggtggat accaatgccg aaagccgcct gcaccgtaaa gaagcgcgtg aagtggcgcg
    43681 cgaaagcctg gtgttgctga aaaaccgtct cgaaacgtta ccgctgaaaa aatcggccac
    43741 cattgcggtg gttgggccac tggcggacag taaacgtgac gtgatgggca gctggtccgc
    43801 agccggtgtt gccgatcaat ccgtgaccgt actgaccggg attaaaaatt ccgtcggtga
    43861 aaacggtaaa gtgctgtatg ccaaaggggc gaacgttacc agtgacaaag gcattatcga
    43921 tttcctgaat cagtatgaag aagcggtcaa agtcgatccg cgttcgccgc aagagatgat
    43981 tgatgaagcg gtgcagacgg cgaaacaatc tgatgtggtg gtggctgtag tcggtgaagc
    44041 acaggggatg gcgcacgaag cctccagccg gaccgatatc actattccgc aaggccaacg
    44101 tgacttgatt gcggcgctga aagccaccgg taaaccgctg gtgctggtgc tgatgaacgg
    44161 gcgtccgctg gcgctggtga aagaagatca gcaggctgat gcgattctgg aaacctggtt
    44221 tgcggggact gaaggcggta atgcaattgc cgatgtattg tttggcgatt acaacccgtc
    44281 cggcaagctg ccaatttcct tcccgcgttc tgtcgggcag atcccggtgt actacagcca
    44341 tctgaacacc ggtcgtccgt ataatgccga caagccgaac aaatacactt cgcgttattt
    44401 tgatgaagct aacggagcgc tttatccgtt cggctatggt ctgagctata ccactttcac
    44461 cgtctctgat gtgaaacttt ctgcgccgac catgaagcgt gacggcaaag tgacggccag
    44521 cgtgcaggtg acgaacaccg gtaagcgcga gggggccacg gtagtgcaga tgtacttgca
    44581 ggatgtgacg gcttccatga gtcgccctgt gaaacagctg aaaggctttg agaaaatcac
    44641 cctgaaaccg ggcgaaactc agactgtcag cttcccgatc gatattgagg cgctgaagtt
    44701 ctggaatcaa cagatgaaat atgacgccga gcctggcaag ttcaatgtat ttatcggcac
    44761 tgattccgca cgcgttaaga aaggcgagtt tgagttgctg taatgcttta gtaagggcgc
    44821 gacgtttgcg ccctttgcct gatgttcata tcttaacttg ctgaattttc gccctttgta
    44881 ggccggataa ggcgctcgcg ccgcatccgg caggaaacgc cggaccgaat atgcctctta
    44941 agtatttatc tgattctgca actgaatcct tccgctcagg ctaactctgt cattatcaac
    45001 tatgcttttc tcttaattcg ctgaaaacaa cagtaaaaga atgaggaaag catcatgcca
    45061 ctcttaaagc tctgggcagg ttcactggtt atgttggcag ccgtgagcct gccgctacag
    45121 gcggcttccc ccgttaaagt cggttcaaaa atcgataccg aaggcgcgct gctcggcaac
    45181 atcatcttgc aggtactcga aagccacggc gtaccgacgg tgaataaagt gcaactcgga
    45241 acgactcctg tggggcgcgg ggcgattact tccggtgaac tggatatcta tccggaatat
    45301 accggcaatg gcgctttctt ctttaaagat gaaaacgatg cagcgtggaa aaatgccggg
    45361 cagggctacg agaaagtcaa aaagctcgat gcggagcaca acaagttaat ctggctgacg
    45421 cccgcgcctg caaataacac ctggactatc gccgtgcgtc aggatgtggc agagaaaaac
    45481 aaactcactt cgcttgccga cctgagtcgt tatctgcaag agggcggcac cttcaaactg
    45541 gcagcctcgg cagagtttat cgaacgcgcc gatgcgttac ccacgtttga aaaagcctat
    45601 ggctttaagc tcggtcagga tcagttgctg tcactggctg gtggcgacac ggcggtgacg
    45661 attaaagccg ctgcccagca aacctctggc gttaatgccg caatggctta cggcactgac
    45721 ggtccggtcg cggcgctggg gctgcaaacc ttaagcgatc cgcaaggcgt gcaacctatc
    45781 tacgcgcctg caccagtggt gcgtgaatcg gtgttgaagg agtatccgca aatggcacag
    45841 tggctacagc cagtcttcgc cagcctcgat gcaaaaacat tgcagcaact gaatgccagc
    45901 attgcagtgg aaggactgga tgccaaaaaa gtggctgccg actacctgaa acaaaaaggg
    45961 tgggcgaagt aatttcccgt gacttatcta cgtattaatc ctgttctggc gctgctgttg
    46021 ttgctgacgg caatcgcagc ggcgctgccg tttatcagtt acgcgcctaa tcgtttagtc
    46081 tcgggtgaag ggcttcatct ctggcaactg tggccgcaaa cgatctggat gctggtgggc
    46141 gttggttgcg cctggctgac agcctgtttt gtccccgcta aaaaaggcag catttttgca
    46201 ctcattctgg cgcaattcgt cttcgtattg ttggtgtggg gagctggaaa ggcggcgacc
    46261 caactggcgc aaaatggcag tgcgctggcg cgtaccagcc tcggcagagg tttctggctg
    46321 gctgcggcgc tggcattgct ggcctgtagc gatgccatcc gccgaatctc cacgcatccg
    46381 ctgtggcgct ggttgttgca tatgcagatt gccattattc cgctgtggtt gctgtactcc
    46441 ggcacgctta acgatctctc actaatgaaa gaatacgcca accgtcagga tgtgtttgac
    46501 gacgcgctgg cacaacatct gacgttgctg tttggtgcgg tgctgcctgc gttagtgatt
    46561 ggtgtgccgt tgggcatctg gtgctacttt tccactgcgc ggcagggggc aattttttct
    46621 ctgctcaatg tcattcagac cgtgccttcg gtggcgctct ttggcctgtt gattgcgccg
    46681 cttgccgcgc tggttacggc ctttccgtgg ctggggaagc tcggcatagc aggaaccgga
    46741 atgacacccg cactgattgc gctggtgctc tatgccttgc tgccgctggt gcgaggcgtg
    46801 gtagtcggtt tgaaccagat cccgcgcgat gtgctggaga gcgccagagc gatggggatg
    46861 agcggggcgc agcgtttcct gcatgttcag ttaccgctgg cgttactggt atttctgcgc
    46921 agcctgcggg tggtgatggt gcaaactgta ggtatggcgg tgattgcggc gttaatcggc
    46981 gcaggcggtt ttggtgcgct ggttttccag gggctgctaa gcagcgccat tgatttagtg
    47041 ttgctggggg tgatcccggt aattgttctg gcggtgctta ccgacgcgct gttcgatttg
    47101 cttatcgcac tgctgaaggt gaaacgtaat gattgaattt agccatgtca gcaaactgtt
    47161 cggcgcacaa aaagccgtta acgatctcaa tctcaatttt caggaaggga gtttttcggt
    47221 gctgattggc acatctggct ccggcaaatc caccaccctg aaaatgatta accgcctggt
    47281 ggagcatgac agcggagtga tccgctttgc cggagaagaa attcgctcgc tgccagtact
    47341 ggagttgcgc cgccggatgg gctatgccat tcaatctatt ggcctgttcc cccactggag
    47401 cgtggcgcaa aacatcgcta ccgtgccgca attacaaaaa tggtcgcggg cgcggattga
    47461 cgatcgtatc gacgaattaa tggcgctact ggggctggag tcaaatttgc gtgagcgtta
    47521 tccgcatcag ctttccggtg gtcagcagca acgtgtggga gtggcgcgtg cactggctgc
    47581 cgatccgcaa gtcttactga tggatgaacc ttttggcgca ctggacccgg taacgcgcgg
    47641 cgcgttgcaa caagagatga cgcgcattca ccgtttgctg gggcgcacta ttgtgctggt
    47701 cactcatgat attgatgagg cgctacggct ggcagaacat ctggtattga tggatcacgg
    47761 tgaagtggtg cagcagggga atccgctgac gatgctgact cgtccggcga atgattttgt
    47821 ccgccagttt tttggacgta gtgaactggg ggtgcacctg ctttcgttac gtagcgtggc
    47881 ggattacgtg cgtcgcgaag aacgggcaga aggtgaggca ctggcagaag agatgacgct
    47941 acgcgatgcg ctctccctgt ttgtcgcgcg gggatgcgag gtgctgccgg tggtgaacac
    48001 gcagggccag ccttgcggca cgctgcattt tcaggatctg ctggtggagg cgtaagcgta
    48061 tgaagatgtt gcgcgatccg ctgttctggc tcattgctct ttttgtggcg ctgattttct
    48121 ggctgcctta cagccagccg ctgtttgctg ccttgttccc acaactgcca cgacccgttt
    48181 atcagcaaga aagttttgca gctctggcac tggctcattt ctggctggtg ggaatttcga
    48241 gtttgtttgc ggtgatcatt ggcacaggtg ccggaattgc tgttactcgc ccgtggggcg
    48301 cggaatttcg cccactggtg gaaactattg ccgccgttgg acagactttt ccgcctgtcg
    48361 cagtgctggc gattgccgtt ccggtgatcg gctttggtct gcaaccagcg attatcgcct
    48421 tgatccttta cggtgtgctg cccatcctgc aggtgacact tgccgggctg ggagcgattg
    48481 atcccagcgt gacagaagtt gcgaaaggta tgggaatgag tcgaggtcag cgactgcgta
    48541 aggtcgagct accgctggcg gctccggtga ttctggcggg cgtgcgaact tcggtgatta
    48601 tcaacattgg tacggcgacg atcgcctcaa cggtaggggc cagtacgctg ggtacgccca
    48661 tcatcatcgg gcttagcgga tttaataccg cgtatgtgat ccagggggcg ttactggtgg
    48721 cactggcggc gatcatcgca gaccgcctgt ttgaaaggct ggtgcaggcg cttagccagc
    48781 acgcaaaata aaggtataac ctgcgagcat gacgccacca attccgccta acgccataaa
    48841 caggaacagg gcgatgaccc caattttagc tatgcgcata atgcactcct tatgttaacg
    48901 aaaggattgt acagtaaagc gcatttgtta acgaatcatt aaataccgag tgggaaaata
    48961 tcatggcctt gttcttgcca actggtgagt tgctgctgtt gggcggaggt tcgattttca
    49021 ccgcaccaca ccagcaatgt acg
//
LOCUS       DAJVPP010000016        48179 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVPP010000016 DAJVPP010000000
VERSION     DAJVPP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.016"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            13..306
                     /locus_tag="OQM34_000935"
     CDS             13..306
                     /locus_tag="OQM34_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1451481.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(347..1537)
                     /gene="nepI"
                     /locus_tag="OQM34_000936"
     CDS             complement(347..1537)
                     /gene="nepI"
                     /locus_tag="OQM34_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCS1451482.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1748..2200)
                     /locus_tag="OQM34_000937"
     CDS             complement(1748..2200)
                     /locus_tag="OQM34_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS1451483.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2253..3587)
                     /gene="adeQ"
                     /locus_tag="OQM34_000938"
                     /gene_synonym="yicO"
     CDS             complement(2253..3587)
                     /gene="adeQ"
                     /locus_tag="OQM34_000938"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1451484.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3762..5528
                     /gene="adeD"
                     /locus_tag="OQM34_000939"
     CDS             3762..5528
                     /gene="adeD"
                     /locus_tag="OQM34_000939"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS1451485.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAKEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5574..6965)
                     /gene="uhpT"
                     /locus_tag="OQM34_000940"
     CDS             complement(5574..6965)
                     /gene="uhpT"
                     /locus_tag="OQM34_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1451486.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7103..8422)
                     /gene="uhpC"
                     /locus_tag="OQM34_000941"
     CDS             complement(7103..8422)
                     /gene="uhpC"
                     /locus_tag="OQM34_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1451487.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8432..9934)
                     /gene="uhpB"
                     /locus_tag="OQM34_000942"
     CDS             complement(8432..9934)
                     /gene="uhpB"
                     /locus_tag="OQM34_000942"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1451488.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(9934..10524)
                     /gene="uhpA"
                     /locus_tag="OQM34_000943"
     CDS             complement(9934..10524)
                     /gene="uhpA"
                     /locus_tag="OQM34_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1451489.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10599..10889)
                     /gene="ilvN"
                     /locus_tag="OQM34_000944"
     CDS             complement(10599..10889)
                     /gene="ilvN"
                     /locus_tag="OQM34_000944"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1451490.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10893..12581)
                     /gene="ilvB"
                     /locus_tag="OQM34_000945"
     CDS             complement(10893..12581)
                     /gene="ilvB"
                     /locus_tag="OQM34_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS1451491.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12687..12785)
                     /gene="ivbL"
                     /locus_tag="OQM34_000946"
     CDS             complement(12687..12785)
                     /gene="ivbL"
                     /locus_tag="OQM34_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1451492.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13350..13439
                     /gene="tisB"
                     /locus_tag="OQM34_000947"
     CDS             13350..13439
                     /gene="tisB"
                     /locus_tag="OQM34_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1451493.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13719..14903
                     /gene="emrD"
                     /locus_tag="OQM34_000948"
     CDS             13719..14903
                     /gene="emrD"
                     /locus_tag="OQM34_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1451494.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(14911..15408)
                     /locus_tag="OQM34_000949"
     CDS             complement(14911..15408)
                     /locus_tag="OQM34_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1451495.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15405..15767)
                     /locus_tag="OQM34_000950"
     CDS             complement(15405..15767)
                     /locus_tag="OQM34_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1451496.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15757..16104)
                     /locus_tag="OQM34_000951"
     CDS             complement(15757..16104)
                     /locus_tag="OQM34_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS1451497.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16213..16626
                     /locus_tag="OQM34_000952"
     CDS             16213..16626
                     /locus_tag="OQM34_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451498.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16673..18115)
                     /locus_tag="OQM34_000953"
                     /pseudo
     CDS             complement(16673..18115)
                     /locus_tag="OQM34_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18150..19865)
                     /locus_tag="OQM34_000954"
     CDS             complement(18150..19865)
                     /locus_tag="OQM34_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCS1451499.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20032..20925
                     /locus_tag="OQM34_000955"
     CDS             20032..20925
                     /locus_tag="OQM34_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1451500.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(20922..21736)
                     /locus_tag="OQM34_000956"
                     /pseudo
     CDS             complement(20922..21736)
                     /locus_tag="OQM34_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21736..23352)
                     /locus_tag="OQM34_000957"
     CDS             complement(21736..23352)
                     /locus_tag="OQM34_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS1451501.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23647..24321
                     /locus_tag="OQM34_000958"
     CDS             23647..24321
                     /locus_tag="OQM34_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1451502.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24360..26021)
                     /locus_tag="OQM34_000959"
     CDS             complement(24360..26021)
                     /locus_tag="OQM34_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1451503.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLVLRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26217..26645)
                     /gene="ibpB"
                     /locus_tag="OQM34_000960"
     CDS             complement(26217..26645)
                     /gene="ibpB"
                     /locus_tag="OQM34_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1451504.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26757..27170)
                     /gene="ibpA"
                     /locus_tag="OQM34_000961"
     CDS             complement(26757..27170)
                     /gene="ibpA"
                     /locus_tag="OQM34_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1451505.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27164..27236)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27475..27807
                     /locus_tag="OQM34_000962"
     CDS             27475..27807
                     /locus_tag="OQM34_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1451506.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27809..29071)
                     /locus_tag="OQM34_000963"
     CDS             complement(27809..29071)
                     /locus_tag="OQM34_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS1451507.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTNTNTNTNTWTPDSQWLVF
                     DVRPSGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPD
                     ETWHYDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYN
                     DHVMHQLDPALNLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEI
                     NRAYEEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTL
                     PAPPRGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISP
                     QGGEPRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPS
                     ADAVVFSPDGQWLAWMEGGQLWITETDR"
     gene            29172..30272
                     /gene="cbrA"
                     /locus_tag="OQM34_000964"
     CDS             29172..30272
                     /gene="cbrA"
                     /locus_tag="OQM34_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCS1451508.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30233..31570)
                     /locus_tag="OQM34_000965"
     CDS             complement(30233..31570)
                     /locus_tag="OQM34_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1451509.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31645..32793)
                     /gene="dgoD"
                     /locus_tag="OQM34_000966"
                     /pseudo
     CDS             complement(31645..32793)
                     /gene="dgoD"
                     /locus_tag="OQM34_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32790..33407)
                     /gene="dgoA"
                     /locus_tag="OQM34_000967"
     CDS             complement(32790..33407)
                     /gene="dgoA"
                     /locus_tag="OQM34_000967"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCS1451510.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33391..34269)
                     /gene="dgoK"
                     /locus_tag="OQM34_000968"
     CDS             complement(33391..34269)
                     /gene="dgoK"
                     /locus_tag="OQM34_000968"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCS1451511.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34266..34955)
                     /gene="dgoR"
                     /locus_tag="OQM34_000969"
     CDS             complement(34266..34955)
                     /gene="dgoR"
                     /locus_tag="OQM34_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCS1451512.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35233..35889
                     /locus_tag="OQM34_000970"
     CDS             35233..35889
                     /locus_tag="OQM34_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451513.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGCSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(35935..36747)
                     /gene="yidA"
                     /locus_tag="OQM34_000971"
     CDS             complement(35935..36747)
                     /gene="yidA"
                     /locus_tag="OQM34_000971"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCS1451514.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36861..37259)
                     /locus_tag="OQM34_000972"
     CDS             complement(36861..37259)
                     /locus_tag="OQM34_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCS1451515.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37499..39913)
                     /gene="gyrB"
                     /locus_tag="OQM34_000973"
     CDS             complement(37499..39913)
                     /gene="gyrB"
                     /locus_tag="OQM34_000973"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1451516.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(39942..41015)
                     /gene="recF"
                     /locus_tag="OQM34_000974"
     CDS             complement(39942..41015)
                     /gene="recF"
                     /locus_tag="OQM34_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1451517.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41015..42115)
                     /gene="dnaN"
                     /locus_tag="OQM34_000975"
     CDS             complement(41015..42115)
                     /gene="dnaN"
                     /locus_tag="OQM34_000975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1451518.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42120..43523)
                     /gene="dnaA"
                     /locus_tag="OQM34_000976"
     CDS             complement(42120..43523)
                     /gene="dnaA"
                     /locus_tag="OQM34_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1451519.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43820..43900
                     /locus_tag="OQM34_000977"
     CDS             43820..43900
                     /locus_tag="OQM34_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451520.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44130..44270
                     /gene="rpmH"
                     /locus_tag="OQM34_000978"
     CDS             44130..44270
                     /gene="rpmH"
                     /locus_tag="OQM34_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1451521.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44287..44646
                     /gene="rnpA"
                     /locus_tag="OQM34_000979"
     CDS             44287..44646
                     /gene="rnpA"
                     /locus_tag="OQM34_000979"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1451522.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44610..44867
                     /gene="yidD"
                     /locus_tag="OQM34_000980"
     CDS             44610..44867
                     /gene="yidD"
                     /locus_tag="OQM34_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1451523.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44870..46516
                     /gene="yidC"
                     /locus_tag="OQM34_000981"
     CDS             44870..46516
                     /gene="yidC"
                     /locus_tag="OQM34_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1451524.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46622..47986
                     /gene="mnmE"
                     /locus_tag="OQM34_000982"
     CDS             46622..47986
                     /gene="mnmE"
                     /locus_tag="OQM34_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1451525.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11933 a        13027 c        12185 g        11034 t
ORIGIN      
        1 aaggttgaat ccatgaaacc aacgacacta ctgcttattt tcactttttt cgctatgcca
       61 ggcatcgtat acgccgagtc gccttttagc tcactgcagt ccgcaaaaga gaaaaccacg
      121 gtattacaag atttacgtaa aatttgcacg ccacatgcgt cattatcaga tgaagcgtgg
      181 gaaaagttaa tgttgtcaga tgaaaacaat aaacaacata tacgcgaggc catcgtggcg
      241 atggaacgta ataatcagag caattattgg gaagcgttgg gtaaggtaga atgcccggat
      301 atgtaagaac agcaacaact actgaccaac gcttctttac ctgatatcag gatttcttca
      361 ttttcacctt tgcagtaacc aacaatgcag tcagcaacat caatgtgccg gacaacatca
      421 acggcgaagt cagaccaata ttatccagcg cataaccgcc gattgccgcg ccacaggtat
      481 tagcaagctg aataaccgcc acctgaatag acccggcttt ttctgcctga tcggccagcg
      541 agcgggtgat ccacgttgac cagccgacgg gaaccaatgc aaaagttagc ccccagataa
      601 tcgccacgcc ggtagcaacg attttatcgc ttccccacaa cgtcagtacc aacgcactca
      661 cagccagtat taacggcgcg cctgctaagg ccagttttac cgaacgttta agaatgaacg
      721 acgaaagcga cgtaccaata aagctggcga taccaaaact caacagcacc agcgttaagc
      781 catccacgcc gaatcccgcc aggttcatat acaccgggcg aatatacgtg aagaaagcaa
      841 actgcccggc gaaagacatg aagatggcga tcatccctgc catcacaccc ggacgttgta
      901 ataagcggaa agtattttgt ttctgatgcg agggttcgcc tggcagtgaa ggcaatgatt
      961 tgataatcca gaaaatacac agcacgccca tcaccgccgc cgcattaaag acattgcgcc
     1021 aaccgataag ctcgcctaaa aaactgccca acggcgcggc aatcaccagc gcaatagaaa
     1081 ccgcgccgaa gatcaccgac agcgccttcg gcaccgtacg cggcggtacc agacgcatgg
     1141 tcagcgacgc cgacatcgcc cagaacccgc ccagcgccag ccccagacag gcacgaccga
     1201 ttaaaagcaa actgaatgag ttagcaaagg aaaccagcaa gcaggagagc gtcagcaaaa
     1261 cggcaaacaa aataacaacg tagcggcggt cagtggcctg aattgtctgg gtaataaaca
     1321 aactggcaaa cattgccaca aaggcggtca cggtcaccga ttgcccggca accccttccg
     1381 aaatgcctaa atcctgggcc attggcgtca acaaactgac gggcaaaaac tcaacgataa
     1441 tcagacaggc gacacaaaac gccaccgaga aaacggctga ccagttcggt cgggtgatgg
     1501 catccgcgcc gcggttttcg gcaataaatt cactcatggt gttacccgtg gcatgtttca
     1561 ggggaaaagc cgaccacttt aacattgaaa tgtgtgacga atttaacgtt tttgcaactt
     1621 ttacggtgtt gcttgagctg gagttaagat cgaaacggaa cagggcaaag attaccggat
     1681 gcggcgtgaa cgtcttatcc acccgacagg tcaggcgcgg tctgtaggct gataagacga
     1741 aacggtttca atgcaaccag tgaacgccct cggcaggctg aaagaacgcg ttatagcgca
     1801 tctgaaaagc attaatatcc tgcatttcag gttcggtttc acgcagaaaa cccagcgcca
     1861 gcctgacctg agcatcggta atcggcgcgg aaaggcgggc tttttgtaaa atccggtgcc
     1921 attgcagcag gttctgttcg ctatcatcga caatgacaat ttgccagata agcaacacct
     1981 gcgcggcgtt ttgcaggtgc gactcatccg ggcgatgcaa ataacgtaaa aattcgtcac
     2041 cggctgactt tcccatttga tcgaccatcg actccacggg tacgacatcg atattcagcc
     2101 gatcgctgag acggcgaatc gcttttctgg ccccggatgt cagcacccga aaaccaacca
     2161 caaacactac cgccagcgtg gcgagcatta tccagatcat gagttctcct gcgtaatcag
     2221 aactcatgat atctggaaac cctcgccagt tactaatcca ccagaataat cttcaatgcg
     2281 aacagagctg cgaccaccac cacacacagg ttcagatcgc gccagcgccc ggtgcacact
     2341 ttcatgatgc agtacgacat aaagccgagt gcaatccctt cggtgatcga gaaagtaaag
     2401 ggcatcatca ccgtggtaat aaacgcaggc accgattcgg taaaatcatc ccagttaacg
     2461 cgcgccaggc tcgaagtcat cagcacgcca acaaagatta acgctccagc ggttgcgtaa
     2521 ggaggaacca tcgccaccag cggtgagaag aacataacta acaggaacat aacgccgacc
     2581 acgacagccg ttagccccgt gcgaccaccg actgccacac cagaagtact ttcaatatag
     2641 gcagtaacag acgaggtacc gataaatgcg cctgccaccg aactgacgct atcaacatac
     2701 agcgccttat tcatattggg gaatttaccg ttgctatcta ttaagcccgc tttatcagtt
     2761 acaccaatta atgttcctga tgaatcaaat aggttgatca gcataaagga gaaaatgata
     2821 ccggcgagtt caagtgttaa cgcgccgctc aaatctactt caccaatgac gccgctaata
     2881 tcaggcggaa tggaatagac gccgctaaaa tgaacatcac cgaaaaataa tccacagcaa
     2941 gacgtcacca caatagaaac cagcaccgcg gcatgaaaat gacgtgatga caacacggtt
     3001 ataataaaaa accctaaaat acctaacaac acgccgtgag aacttaaatc gccaatcatc
     3061 accagcgtgt ctttattggc gacaataaca ccagtgtttt ttaatcccat taaggcaata
     3121 aataatccga ttccactggt gataccaata cgtaaactta atggaatgtt ggagatcatc
     3181 cagtaccgga tacgaaacag cgtgagcaaa aatagcccaa ttgcgcccca gaatatcgcg
     3241 cccatcccgg tctgccagga gatccccatc gcccccacga ccacgaacgc aaagaaggcg
     3301 ttcagcccca ttgccggagc cagcgccacg ggtaagttag caaatatccc catcgcaata
     3361 ctgccgatac cggcaatcaa acaggtggta acaaacacca ctttcgggtc catttgtgcc
     3421 gcgccgagga tttgcgggtt cacaaaaaca atgtacacca tggttaaaaa agtggtcatc
     3481 cccgcaatca attctgtgcg gacggtggtc ccatgctgag gtagtttaaa taatcgcgaa
     3541 agcgtccctg attcattact cacgtaatcg gtattgtcat tattcatttt tttgtccatt
     3601 ggaggagatt taatcccttg cttttgtaaa acaaatgaca acaacggttc agtgataatt
     3661 atcacatttc aattgcacat taatagatat tctttaataa tctcgcgacg tttctttatg
     3721 ataaataata atcaaattga taaaatcaaa atgagaaaaa tatgaataat tccattaacc
     3781 ataaatttca tcacattagc cgggctgaat accaggaatt gttagccgtt tcccgtggcg
     3841 acgctgttgc cgattatatt attgataatg tctctattct cgacctgatc aatgccggag
     3901 aaatttccgg cccaattgtg attaaaggac gttacattgc cggtgttggc gcagaatacg
     3961 ctgatactcc ggctttgcag cggattgatg ctcgcggcgc aacggcggtg ccagggttta
     4021 ttgatgctca cctgcatatt gaatccagca tgatgacgcc ggtcactttt gaaaccgcta
     4081 ccctgccgcg cggcctgacg accgttattt gcgaccctca tgaaatcgtc aacgtgatgg
     4141 gagaagccgg attcgcctgg tttgcccgct gtgccgaaca ggcaaggcaa aaccagtact
     4201 tacaggtcag ctcttgcgtg cccgctctgg aaggctgcga tgttaacggt gccagtttta
     4261 cccttgaaca gatgctcgcc tggcgggacc atccgcaggt taccggcctt gcagaaatga
     4321 tggactaccc tggcgtaatt aacgggcaga atgcgctgct cgataaactc gatgcatttc
     4381 gctacctgac gctggacggt cactgtccgg gtttgggtgg caaagaactt aacgcctata
     4441 ttgctgcggg tattgaaaac tgccacgaaa gttatcagct ggaagaagga cgccggaaat
     4501 tacaactcgg catgtcgttg atgatccgcg aagggtccgc tgccagcaat ctcaacgcac
     4561 tggcaccgtt gatcaacgaa tttaacagcc cgcaatgcat gctctgtacc gatgaccgta
     4621 acccgtggga gatcgcccat gaagggcata tcgatgcctt aattcgccgc ctgatcgaac
     4681 aacacaatgt gccgctgcat gtggcatatc gcgtcgccag ctggtcgacg gcgcgccact
     4741 ttggtctgaa tcacctcggc ttactggcac ccggcaagca ggccgatatc gtcctgttga
     4801 gcgatgcgcg taaggtcacg gtgcagcagg tactggtgaa aggcgaaccg attgatgcgc
     4861 aaaccttaca ggcggaagag tcggcgagac tggcgctgtc tgccccgcca tatggcaata
     4921 ccattgcccg ccagccagtt tccgccagcg actttgccct gcaattcacg cccggaaaac
     4981 gctatcgggt cattgacgtc atccataacg aattgattac gcactcccgc tccagcgtct
     5041 acagcgaaaa tggttttgat cgcgatgatg tgtgctttat tgccgtactt gagcgttacg
     5101 ggcaacggct ggctccggcc tgtggtttgc tcggcggctt tggcctgaat gaaggcgcgc
     5161 tggctgcgac agtcagccat gacagccata atattgtggt gatcggtcgc agtgccaaag
     5221 agatggcgct ggcggtcaat caggtgattc aggatggcgg cgggctgtgc gtggtacgta
     5281 acggccaagt acaaagtcat ctgccgttac ccattgccgg gctgatgagc accgacacgg
     5341 cgcagtcgct ggcggaacaa attgacgcct tgaaagccgc cgcccgtgaa tgcggtccgt
     5401 tacccgatga gccgtttatt cagatggcgt ttctttctct gccagtgatc cccgcgctaa
     5461 aactaaccag tcaggggcta tttgatggcg agaagtttgc cttcactacg ctggaagtca
     5521 cggaataata aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc
     5581 actgtcaact gctgaatttt tttctcgcgg cggattttgc gttcttccat taccgccact
     5641 atcgccatca ggcagataca accaatcgcg gcgatatcca gcgcggcgaa ggtgcctgcc
     5701 cagccggtaa ggccgaatac cggcgtccca tcggcaatca ttcccagacc taacttggca
     5761 aagctgtcac caatcaggta agcaaaggtg cctttaatac catcggcagc gccaatcgct
     5821 tttttaggta caaagccaac agcagccaca ccaatcaaca attgcgggcc aaagaccagg
     5881 aaacccaacg caaagagaga agccagatag atatattcgt tactggcatg ttgatacaca
     5941 ccgagcgtgg cgataatcag cgccagcgcg atgcaggcca ccaggccacg gcgaccgttc
     6001 gccaggtcag agagccagcc ccacagcagc gtaccgacca gcgcaccagc ttcaaacagc
     6061 gtaaagccct gaatcgccac cgctttagag agtttcagtt cctggaacgc gtatacggtt
     6121 gaccactggt cgataccaat acgtaccaca tagaggaaaa tgttggcgaa gcacagcagc
     6181 cagatcactt tgtttttcag cacatactca acaaagatct gccacttggt catatcggta
     6241 gattctgtct ctttgtcctc ttcgctgatc tcctcgccga acagttcttc agctttgccg
     6301 aggccataag attccgggga gtcgctgccg taacgcaggc cgataaaacc gacaatcagc
     6361 gcgataatcg acgggaagat aaacatgccg atgacatggc catcgaacag gtaatttgcc
     6421 ccgaacagcg ccacacctgc tgcgcctgca ccgccaaggt tgtgagaaat attccagaaa
     6481 ccgaggaatg tcccgcgttt acgacgcggc gtccatttgg tgatggtgga gtaactgcac
     6541 gaaccgccgg tactctggaa aaagccgctt aaggcgtaga aggcaatcat caggaacagg
     6601 ctaaccgagc cgctgcccat actggcactg aagcccagca tacaaatggc agagaggata
     6661 agcatgaacg gcaggaattg tttggtgttt ttgccgtcgg cgtagtatga aaccaacgtt
     6721 ttacccacgc cataagtgat ggagaaaccc aggccgatca tccccagctg cgtcatgctc
     6781 aacccgtagg tcgaaatcat atcgttctgc gcgatgttaa agttcttgcg aatcaggtac
     6841 atcgtcaggt agccgataaa gaccaccagg taggattgca tgaacggttt gaaccacatt
     6901 ttgcgccgca cttcgagcgg aaggtccagg gtcggcttgc gaacctggtt taggaaagcc
     6961 agcatgggtt actcctgaaa tgaatacctg cgtgaggcat gcattgtaaa aatcagcagc
     7021 aaatcaggcc tgagatagcg tccaagaaaa acctggaaaa tttcttagtt ttgccggata
     7081 tgaagtgaaa aggtgagatg catcacgctt cacgcggtgt ctgggcgttc aaaaagggca
     7141 gtaacagcag tgcggaaatc ccggcggcga tagagataac cacaaaaaat ccgctccagt
     7201 gccaggtatc gagcactttc gccagcggcc aaccagcaag cgacgccccc agataagcaa
     7261 acaagccgac aaaccccgtc gccgcccctg ccgcctcttt gtgggaacac tctgccgccg
     7321 ccataccaat taacatctgc gggccaaaga caaaaaaacc aatggtgaag aagcaggttg
     7381 cctgcatcac gtagctggca aatggcatca gccacagcga gccgactgaa agcaaaattc
     7441 cggcggcgaa aatcaaattc atcggcccac ggttgccgtt aaacaatttg tccgagcccc
     7501 aaccggctac cagcgcaccg ataaatccgc ccagttcaaa catcgtcact gccgtattcg
     7561 ccgtgaccag atcgacgccc agcgtctcgg acatatacaa attgccccag tcgttgatcg
     7621 ccgcccggac cacatagacc agcacatagc aaaacgaaag cagccagata tacggattca
     7681 gcaacacata tttggtgagg atctctttac gcgtcaaccc tgccccttct tgttgttgag
     7741 caatttccag cgcgtcgtgc cgccattcac cgaccgccgg taaacctaac gcctgcgggc
     7801 gatcgcgtag ccgccagcag agaaaaatcc ccacgactat cgccatacaa ccagcaatca
     7861 tcatcccggc acgccagccg taatgcagcg cgctcgctgc catcacaatg ggaatgagtg
     7921 cgccgccgac gttatgcgcc gtgttccata atgcccacca accgccgcgc tcggtacgtg
     7981 aataccaggc cgttaacaga cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt
     8041 tcagcaccca gagcacggca aacgcccata gcgacgtcga gaagccaaac agaatgttca
     8101 tgatgcccgt ggcgataagc cctatcccca taaaataacg ggcatttgag cgatcgctga
     8161 caatgccgga gacaaacttc gacacgccgt aagtaatgta aaacagggtc gctaacaggc
     8221 cgatatcgct acggctgagc acgccgttag caaggatttc tggtaccgcg gcgttaaaac
     8281 ttttccgcgt gaagtaaaac agcgcgtaac ccagccagat ggtcagcaga atatgccgac
     8341 gccagtagcg ataacgggca tcaatttcat gtttatcagt cattaatggc gcatcggcag
     8401 gcgctttcag aaacggcaac atcatcgaac cttagacata acgttgaggt agagaaacgc
     8461 tgacacgcgt gccgtgcaga caggaaatgt gtaatgtgcc gcccagcgcc gttacgcgct
     8521 cgcgcattcc ggtgaggcca aaaccttgtt gcccggaacc cggcggcaaa ccgctgccat
     8581 cgtcttcaat caccagcatc aaccgttcat cctgctgcca gccttgcagc gtaaccgcgc
     8641 tggcatcggc atgtttcaca atgttgttca gcccttcctg gcagacacga aacagcgtca
     8701 cgcgctggtt ttcgcttaat gctgattcat cgatacgcca ttcgagatgg ctgacaatgc
     8761 cgcgcccttc cagctccatt tcccgcatca gtgagcggat ggcctgctcc agggtgagat
     8821 catccagctg gcgcggacgt aaccgtccta acaaacggcg caccgcgtcg taaacgccca
     8881 gcgacagttg ttcgatgagc tgcccgctct gcttcacact ggcgttatct gccgccagcc
     8941 gctgaacaat gcccgcctga gtacgaatag cagtgatagt ctgaccgata tcgtcgtgca
     9001 gctcacgcgc cacgtcgcgg cgcacgctct cttcggtttc taacaaacgt tcagccagat
     9061 gctgattgcg cgccagttcc ttttgtagcg actggttaag ttcacgcaac cgctggatgc
     9121 ctgcgcccag taacaaccct gtcagacttt gcaccagcaa cgagagtaat aaatccacca
     9181 gatgatcgcg ccaggtttga ctggcgatca gcgcgatggc gttcatcaac gtcgcaatca
     9241 gcgctccttg ccagccatag tgccaggcca gcgcgataat cggcagcgcc agacagaatg
     9301 gcgtaaagcg cgacagttcg tccggcaatc ccaactggag ccagagactg ataacaaaca
     9361 gcagcaagta ccagaccaga tgccgcccgc gccagttgat gggctgagaa accagtgacg
     9421 ggccgagcgg cagccaggtg ttattggcga gatagtgcca gaacaccaga catatcgggg
     9481 ccagcgtcag accgccagtt aaagtcagca acagcgcatt ccacgactct ttgccgtgcc
     9541 aaagccaggg cagcgactgc aacaacgccg ccgccgttaa cgccgcaccc tgtaacagca
     9601 aggtgcgcca gtcacgctga tggcgatagc gcgagatcag cgctacgggc agtaacgtca
     9661 gtaaactacc gatcatcaat aacggaaaat gggttaaacc gaccgcctgc gttagccagt
     9721 aaatcagcag ccactccgca cccagcaata cgggccagta tccgcgcggg cattgcagca
     9781 ttagccccag acgcagacca aacggaaata acagcaccgc catatcaggg cgctcaacca
     9841 gatgcaggct gatactccat aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa
     9901 taacggtaat taagcgggag aacaacgtct tcatcaccag ccatcaaaca tgcggcgcgc
     9961 cagctctacg tcgttactga cgcccagttt ttccatcaga ttggcgcgat ggacgtgtac
    10021 cgttttcggt gacaagccca gttcggcggc aatctctttc accgccattc cttgcgccag
    10081 tttttccgcc acctggcgtt cacgtttggt cagcgggtcc tgacgaccgg atgccagttt
    10141 aatggcaata tccggcgtca gataacagcc gcccgtggca accgtatgca ccgcagcaat
    10201 gagttcatcc gggctacagc gtttggaaag aaagccgcgt gcccccgcgt taagcgcctg
    10261 ctcaaccagc gcaggactgt cgtgaacgga gagcataatc gtcgccatac ctttcggcag
    10321 ctggcttagc agctccagac cggagatatc gggcatggag atatcgcaaa tacacacctg
    10381 cacaccgcgc cccggcagcc ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac
    10441 ctgcaaatca ggttccagcc ccagcagctg cgcaaagccg gagcggacga tgaggtgatc
    10501 gtctataagg gcaacggtga tcatggtctt gtcctggcgg gtaaaaaaaa acgcgcttac
    10561 cttaacgata agcgcgatgt tgttcaagcc ttgagcggtt actgaaaaaa caccgcgatc
    10621 ttgttaaaca tcgtcggatc ggactgatta cgctgcactt tcacgacatc ttccagctta
    10681 gcgatttggc ttatcatctg ctccagacgc tggtcgtcat tgaccagtag ccagatatgg
    10741 cttttgtcgc tgtcctgaat cggcagacaa agaatgcctt caacgttaaa agcgcggcgg
    10801 gcaaaaaggc cacaaacgtg ggtcattacg cccggatggt tgcgaacggt gagctccaga
    10861 attacgttgt tatgagttgt gttttgcatg gcttattccc ccaccatttc agtattcgcc
    10921 gcacctggcg gcaccatcgg gtaaactttt tcttcggcat caatgcgcac atggatcagc
    10981 gccgggccag ggcgattgat gatttcctgc aatgcagcct gcggatcggc ttcgttattc
    11041 aaatcacagg tttcgaggcc gaatccggcg gcaatctgca taaagttgat tttgcccgga
    11101 taggtggcgg caaaaacgcc ttgctcgtag aacagactct gttgctgatg caccagcccc
    11161 agcgcttcgt tgttcatcag aatgattttg acatccagct gattttcact ggcggtcgcc
    11221 atctcctgaa tattcatcat caggctgccg tcgccggaga aacacaacac tttgcgatcc
    11281 gggttcgcca gcgccgcgcc aatcgccgca ggcaggccaa aacccatcgt gcccagccca
    11341 ccggaggtca gccactggcg tgggcgattg agcggataag cttgcgcggt ccacatctga
    11401 tgctgaccca catcggtggt gataatcgca ttgtcatcga cacaggcggc aacggcgttg
    11461 atcaggccgt aatggcttaa tggatcgcac gctttcggga ttggacacgg gaattcgcgc
    11521 tgcaaatccg ctaccaactg gtgccactct gcacgcggtt gcgcttccac ccgcgggatc
    11581 aattgcgcca gcacgtcatc aacatccgcc tgaatcgcca catgcggctg cttgatttta
    11641 cccagctctg cgcgatcgat atcgacatga ataattttgg cattcggaca gaactgctcg
    11701 gttttgccaa tcgcccggtc atcaaaacgc gcaccgagca caatcaacaa atccgcctcc
    11761 tgcaaaatat agttggtact gcgcacgccg tgcatcccca gcatacccag cgacaacgga
    11821 tgcgcttttg gcagcatgcc cagcgccatt aaagtcatgg tggtaggcag ttgcgctttc
    11881 tccgccagtt cacgcacccg cgcaggcgca ttgatcacac caccacccag ataaagcacc
    11941 gggcgtttgg cagcgttaat cattgtagct gcgtcacgaa tgctttcttc gctaaaggcg
    12001 ggtgcagcgg ctttttctgc caccgcgggc tgagcttcaa tctcaaaaac cgccgtttgt
    12061 acatccttag gaatgtctat ccacaccggg ctagggcggc ctgattgcgc aatgcggaag
    12121 gcatcgctca tgacctgtgg gagctcttcg atatgtctga ccagatagtt gtgtttggtg
    12181 atggggatag agatgccgta ggtgtccact tcttggaagg cgtcggtgcc gatcatcgag
    12241 gcgggaacct gaccagtgat gcaaatcagc gggatggagt ccagccgcgc atcagcaatg
    12301 gcggtcacca ggttagtcgc acccggcccg ctacaggcca tacagaccgc cggtttaccg
    12361 tcggtgcgcg ccattccctg agcgataaac cccgcgccct gttcatggcg agccagaata
    12421 tggcggattt gcgtgctttg gcttaaggcg tcgtaaacag gcaggataga accgcccgga
    12481 atgcccgtca caatcttaat gccctgctgt tccaggaaat gaacgataaa ttctgcgccg
    12541 gtaaagcgct tacgcgtcga tgttgtgccc gaacttgcca tgctccagtc cttttcttct
    12601 gggccgactt tccgggaggt gcttaaacga aaaaccccgc ccggtttgcg ccggcggggt
    12661 tttggaatcg tgtgttgttc cagtccctac ggcgcattgc cgacgaccac caccacacgc
    12721 acgacgacca ctgcggcgga tggcgcagtt ggtagtagtt ttgcgttgaa catggaagta
    12781 gtcattgggg accttgttgg ttttgtgttt aacaatattt atacaagcac agctttacag
    12841 gggagacaat ggaaaatttt tcagcaaggg aaaattgagg ggttgatcac gttttgtact
    12901 gaattgcaga taacaaaaaa ccccgccgga gcgaggtttc gtcagccgcc tgcggctggt
    12961 aaccgcaaag cacactgtat tatgtcaaca ctgaaagtat acgtgttccg cgcagaacgc
    13021 gcaatttcgg cacgaatttt gacgtattta gtgcatagtt gagtatcgtt cacagtttgc
    13081 gttttgtcca aatattactg tttatttata cagtaaactt ctataatatc actgtacgca
    13141 atgtgttatg cgggggccgc atcgttaccc ggcgaactaa gtcctggctg aaacgggtgg
    13201 tgccgtcagc gccttaaccc cgcgtgagca cactgtgtta tgtcaacaag cacaacgttt
    13261 ctccttgaga taccgcgtgc acaacagctg gcaacaggca gcggaaaggt acgtcagctg
    13321 gcagtgctcc tgaaccacag gagacgcgta tgagcctggt ggatatcgcc attcttatcc
    13381 tcaaactcat tgttgcagca ctgcaactgc ttgatgctgt tctgaaatac ctgaagtaat
    13441 tcagattcaa gtcgcaccaa aggggagcgg gtaaccgctc cccttttata tttagcgtgc
    13501 gggttggagt cggatgcgat gctgacgcat cttatccgcc ctaccatccc tcccggcaac
    13561 atttattgcc gcttttgttt acatattctg ccgctaaaca attccccatt cctggcgtat
    13621 atctggctaa cattcatcaa tgtgatagat tcctctcccg catttatggg aatgcgtagt
    13681 gacttattct aattattttt ataaaagcat ccgtgataat gaaaaggcaa agaaacgtca
    13741 atttgttatt gatgttggta ttactcgtgg ccgtcggtca gatggcgcaa accatttata
    13801 ttccagctat tgccgatatg gcgcgcgatc tcaacgtccg tgaaggggcg gtgcagagcg
    13861 taatgggcgc ttatctgctg acttacggtg tctcacagct gttttatggc ccgatatccg
    13921 accgtgtcgg tcgccgacca gtgatcctcg tcggaatgtc catttttatg ctggcaacgc
    13981 tggtcgcggt cacgacctcc agtttgacgg tattgattgc cgccagcgcg atgcagggga
    14041 tgggcaccgg cgttggcggc gtaatggcgc gtactttgcc gcgtgattta tatgaacgga
    14101 cacagttgcg ccatgctaac agcctgttaa acatggggat tctcgtcagt ccgttgctcg
    14161 caccgctaat cggcggtctg ctggatacga tgtggaactg gcgcgcctgt tatctctttt
    14221 tgttggttct ttgtgctggt gtgaccttca gtatggcccg ctggatgccg gaaacgcgtc
    14281 cggtcgatgc accgcgcacg cgcctgctta ccagttataa aacgcttttc ggtaacagcg
    14341 gttttaactg ttatttgctg atgctgattg gcggtctggc cgggattgct gcctttgaag
    14401 cctgctccgg cgtgctgatg ggcgcggtgt tagggctgag cagtatgacg gtcagtattt
    14461 tgtttattct gccgattccg gcggcatttt ttggggcatg gtttgccggg cgtcctaata
    14521 aacgcttctc aacgttgatg tggcagtcgg ttatctgctg cctgctggct ggcttgctga
    14581 tgtggatccc cgactggttt ggcgtgatga atgtctggac gctgctcgtt cccgccgcgc
    14641 tgttcttttt cggtgccggg atgctgtttc cgctggcgac cagcggcgcg atggagccgt
    14701 tccccttcct ggcgggcacg gctggtgcgc tggtcggcgg tctgcaaaac attggttccg
    14761 gcgtgctggc gtcgctctct gcgatgttgc cgcaaaccgg tcagggcagc ctggggttgt
    14821 tgatgacctt aatgggattg ttgatcgtgc tgtgctggct gccgctggcg acgcggatgt
    14881 cgcatcaggg gcagcccgtt taagcgcacg tcaccgcagc atcgtcatca gctccatggg
    14941 agaacgatgc tgctttatca gatcacgcat cacccgcata tgcggtgcgg agtaagaata
    15001 aaacgcctga tagcccgcac aaagcacgct gtctggcgtc tcctgccgag cgtgcgggca
    15061 caggcgatgc cagtcgcacg cctggcagtc acggcgcggt tgcgggtttt cgctgccaca
    15121 ccatttttgt aaggtggcgt caaaaagctc aatcgagata cgactgatat ctgcccgcac
    15181 ccaacactca aacagggcaa ttaaaaattt cccccacagc tctgctgtca ccgaagcggc
    15241 cgtcagatcg ccccggtcat caaattcaag atagggaatg agctgtacgg agcggatgcc
    15301 ccgctgtttg aaattgtgat aaaaatcggc aggttgcaga gcatccttgt actctacaac
    15361 cagcttatgc ctgtcctctt cagcatcaat gacctgactt cctgtcatgc gactctctca
    15421 ataaatacga taagttgatg tatatgcgtt acagcaaaca gtattgcgag agataacacc
    15481 gcgagggaga tcaaaaattt gtcacggact acgtgaaatt gggaaaaatc gctattcgtg
    15541 acatccatta aattgcgatt acgggtgtag tgccagagga tcagcgccac gatggcgagg
    15601 atgccaatcg aaatccagaa taacatgccc gcctggtgcc agttgtgttt gatagccaac
    15661 gccatcaacg cgccgtagcc cagcatggta cgaaaccacg ccagtgatgt gcgctccggc
    15721 tgcagccctg ggtcggcaat gcgtctggct ttacggctat ccggcataca acaccagtcc
    15781 cataacgatc accgccacaa ccatcaatat taagctgatg attaataagc tgttggtgta
    15841 gggcaggtcc tctttcagac gcatcgcttt ttcattgcgt aaccagcgca ggtagccata
    15901 cattgctaac ccgccggaaa acagacacaa caacaacgcc agcagttcac gaatgacggg
    15961 cgtggcgaaa tctggcgcaa gttgatcaag cccaaccccc gccgccagaa atcctagtgc
    16021 ggtacggatc cacgccagaa aagtacgctc attagccagc gagaagcggt aatcgggcgc
    16081 ttctccaagg cgggaaattt tcattgttgc tcctttgcaa tttcactaac gctctatctt
    16141 tagcggtttt ttttaagaat ttcgtccgcc cgtttcctga tatgcttttg ccatccgcta
    16201 caaggaggtc agatgggtat catagcgcaa aataaaatca gttcattggg catgctgttc
    16261 ggtgccatcg ctttaatgat ggggattatc cacttttcat tcggcccttt ttccgcacca
    16321 ccgccaacgc ttgaaagcat cgtggcggac aaaacggctg aaattaaaag aggtcttctc
    16381 gccggaataa aaggcgaaaa aattaccact gtggagaaaa aagaggacat ggatatcgat
    16441 aaaatcctcg atcaaagcgg tatcgcactg gccattgctg cgctactttg cgcatttatt
    16501 ggcggtatgc gtaaggaaaa tcgctggggc attcgcggcg cgctggtttt tggtatcggc
    16561 atcgtgtgca gcattttgct gatattcctg attttttcct tcctgaccgg cggttcgctg
    16621 gtttaagcag cctgaataat gcccgccggg tgatcatcag cgggcattat cgtcagaatt
    16681 tctgtttttt ctcctccacc ttcactcctt gcgtcggtag gccggtgtca tagtcgcgta
    16741 ccactggcga atagccatct tgtgggcgtg ggcgaaacgc ccccatccag cgcggtcgtg
    16801 catctttacg ccacggacgc agactccatt ggtaactgcg gaacggatcg cgaattttgt
    16861 ccatgtaatc caataaggcg tcatgcattt tgctgcgaac gtctgcaaaa cggatatcat
    16921 cgatcaggtt atgcatttca tttgggtcat tacggcgatc gtaaagttca tcactggtga
    16981 agaggttgag caccagttta aagtcatccg tcacccagca acgcaccgga ataaaaccgc
    17041 caaagctgtc atgctcaatc tcgtagcggt taaattccac catcacgccg cgtggctctt
    17101 tcacggcaag gatattttcc cccggcagaa tctctggttt ttcaatatct gccagcgcca
    17161 tcattgtcgg cagtaaatca atatgactga ctggcgtatc gacctgtcgc cgctcccctt
    17221 gcggcgaacg gatgatcagc ggaatacggg tgatgtcgtc atacatcgcc gctcctttac
    17281 tgatcagctt atgtgcgccc atcatttcac cgtgatcgga ggtataaatg acccacgtat
    17341 tttcacgttg ctctggcgtt aaggcattga tgacacgtcc gatttggtca tcaacaaagt
    17401 cattgcaggc aaaatagagc ggatggtgat aaagcccgtc atcaccgact ggcgatggca
    17461 tcgcctgcgc ccataagcgg tgatgttccg gtttgttcgc caggtcatcc tgtgatttct
    17521 cgccaagttc gtagtaaaaa tcagcgtatt tctctaaata ctccaccgga caggtgaacg
    17581 ggtgatgcgg ctcatcatac gaaaccacca tcaggaaggg ttcctcggcg cgcacgggct
    17641 attgcagaaa atctaccgct cgattgctga tgcggtgcgc ccaggtgaag gtttcgtcaa
    17701 tatggttcgc ctgtaaatct tcgacgctgt ttaggccatt gcgccacagg ctgatctctt
    17761 tttccgtcag ttcgctaaga tagttcgccc catcaaacca gtaatcagca tcccactccg
    17821 gcggacactc gccagtgccg aaatagtcat gaccgtcgag atgccatttg ccgatgtaac
    17881 aggtgtgata gccggcatcc ttaaagtagc gccccatagt ggagatgttt ttgcctggcg
    17941 cgacgttgtt ggtccacggg ccggactggt tagcgtagat accggtgaat agtccggccc
    18001 gtgcaggcgt acaaaccggt gaacaagtgt aggcggaatt aaagcgaata ccttccgccg
    18061 ccagactatc aatattttgc gtattcagcg gtttaccgct atagcaaccg accatattgg
    18121 tggcctgggt atcggtcaat tggggcgttt cataggctat ccttcgcgtc gggagtaaag
    18181 gttacggctg ggtcgtgacg atgccaccag ctgtcaaaaa tcaggtagac aatcactacg
    18241 gcagcgatga aataactgat catcgctaac cagcgcgtac cgtagccgcc gaattcagcc
    18301 agcccgacat agacgccaat catcgcgaac aggataccaa ttgacgcgat cttgacgttt
    18361 ttccacggtt tcatgtccac cgcaaacgca tctttgaagg tgaacggcgt ggcgcgcggt
    18421 ttgataaaac cgatcaccag catcacgacc acgttgatgc agaacgtaca ggccagcaca
    18481 tagaggaaat ggaagtcgaa cttcaccaga tagttgatgg tgatgtagct gattatgcca
    18541 atccccatcg ccacttttgc cgccagcgcc gggatgcgcg gaaagaaaaa gcccataatg
    18601 atgatggtaa ccagcggcac gttgtaaatg ccgttgagct gtttcatcca gctatacagc
    18661 ccctgcggcg cgttggcgat ccacggcgct accaaaaccg aaacgatagc gataaagaaa
    18721 ccaaatttgc gcccgacggt gaccagctgc tgcggctcgg cattctggtt aatgatgcga
    18781 cggtaaatac ccatactgaa taatgtactg gcgctattca gaaagccgtt gaaggtactg
    18841 atcaccgcac caaataacac cgcgccgaag aaaccctcca gtggcactgg caaaacgtta
    18901 ttgaccagcg tcgggtaggc catgtcggct ttcggtagat cctgatacag atgaaacgca
    18961 atcaaccctg gcagcaccag taccagcggg tcgagcattt tcagcaccgc cgttaacagc
    19021 gccccctttt gcccttccgc caggcttttt gacgccagcg tgcgttgcac gatgccctga
    19081 tttgtacacc agtaaaaggt gttcaccaga attaaaccgg taaatgccgc gccaatcggc
    19141 aagggatcgg tcgggccacc gactgagttt aatttctcgg cgtgaacggt ggtaatttgc
    19201 tcaatgccct gcataaagct gcccttgcct atcgcgatca ggccaaatac cggcaccatc
    19261 aacccgccga taaccagccc aataccgttg atggagtccg ccactgccat tgcgcgcagt
    19321 ccgccgatca ccgcatacaa aattcccgcc agaccaagca aaattaccag tagccagata
    19381 gccgcaccgt gagaaatctg tagcgattcc ccgacgtgaa acaggctgtt gagcgccaac
    19441 gcgccggagt agagaacaat cggcagaaag cagacgccgg tggcaatgag gaagcagaag
    19501 tcgatgataa tacgcgtcgt tttatcataa cgttcctcca gaaaatcggg gatggtggca
    19561 atgccgcgct tcagatagcg cggtaaaaag attagcgcga ggaagatcag cgtcaccgct
    19621 gaagtcactt cccagcccat caccgacatg ccgcttttgt aggcctgccc ggaaagacca
    19681 accagttgtt ccgtggaaag gttggttaac attaacgaag cggcaataac cggcgctttt
    19741 agtgaacgtc cggcaagaaa atagccttgt tgcgatccgg tatctgtttt gcgaaccttc
    19801 caccaggtga tcaccgccac cagcagcgta aaaccgacaa aactcaagat ttgtaacgaa
    19861 ttcatctcat agcccttatt tatgtttatg tcccggacgc atgaatatag ggagaggtaa
    19921 aatgcgtcgg gtcaggcagt cagattctta aaatacaaac gtcgtatccc tgaacggatt
    19981 gttttctgtt aagttcaggt tgtgagcata attcgccgga gggatattaa aatgaatgga
    20041 aaattgcaaa gttcagatgt aaaaaacgaa actccgtaca atattccctt attgattaac
    20101 gaaaatgtga tctccagcgg aatttctctg atctcgctct ggcataccta cgccgacgag
    20161 cattaccggg tgatctggcc gcgggacaag aagaaaccgc ttatcgccaa ctcatgggtt
    20221 gcggtgtata ccgtacaagg atgtgggaaa attcttttaa agaatggcga acaaataact
    20281 ctgcatagca actgtattat atttttaaag ccaatggata ttcactctta tcactgtgaa
    20341 ggtttagtct gggaacagta ctggatggaa tttaccccca ccagtatgat ggatattccc
    20401 gttggtcagc aaagcgttat ttataatggc gaaatttata atcaggaact caccgaagtt
    20461 gctgagttaa taacttcacc agaagcaata aaaaataatc tggcagtcgc ttttctgacg
    20521 aaaattattt atcagtggat ttgtcttatg tccgcagatg gtaaaaaaga tccacaacgg
    20581 cggcaaattg aaaaattaat tgccacttta catgccagtc tgcaacaacg ctggagcgta
    20641 gctgatatgg ctgccacgat cccctgtagc gaagcctggt tgcgtcgtct gtttttacgc
    20701 tataccggca agacgccgaa agaatattac ctcgatgcgc gtctggatct ggcgctatcg
    20761 ctattaaagc aacaaggaaa ctcggttggc gaagtcgctg atacgctcaa cttcttcgac
    20821 tcctttcatt tcagcaaagc ctttaaacat aaatttggtt atgcgccgtc agccgtgcta
    20881 aagaatacgg accagcaccc aacggatgcc agtccacaca attaacgtaa ctctggccag
    20941 tacgctttgt tggcctccag caactcatcc agaatagctt tggcgaccga ggcgctcggt
    21001 accgttttcg acaacgtaat cgcctgccac aggtgctgat atgaacgctg ttcccaggcg
    21061 tccacgacca gtttttccac cgccacttgc tgactcatta accctttctg aaactgcggg
    21121 atatcgccga ccaccagtgg ttcaggccgt agtagcgcac gcgcatctgt ttgcgatctt
    21181 gcagcccaac aatttgcgcc atccggcttt caataccgat tggcatgtca cagatgttga
    21241 ggattttcgc attcgggcgc agacgacgcg tggcttctgc gacaattgct gccgggttgg
    21301 agtagttgag catctaggcg tttggtgaat atttttccat ataatccacc agttccagga
    21361 cgccgccaat ggaacgcatg ccgtacgcta ttccgccggg tccgcaagtt tcctgaccaa
    21421 caacaccgtg gcgcagtggg attttttcat ccagttcgcg catcgggtac ttgccgacgc
    21481 ggatgtgcgc catgacaaaa tcaacgtcgc tgaacgccac ttcaggatcg gtggtgtaac
    21541 taaacgcaat gtccggcgct ttttctttaa ggatgacttt acaggcttcg gcaatcactt
    21601 cctgccgcgc gccatcgtta tcataaaatt tcagtgcacg aagcgggaaa cggtcctgat
    21661 tcgccaggag catcaacacg atgcctggcg taaaggtgct tccaccgcct gcgacaacca
    21721 ctgagaattt ggtcattata ctgcctccgt aatggcaaca ttttctgctg attgatgaga
    21781 attaattaag ctatcgagct gttcacgcaa ttgggataca tgcagaccaa tgatcacctg
    21841 aatggcatcg ccactacgga agacgccgtg cgctcccagc tttttaaaga cttcgtcatc
    21901 cagcgtttgt gacatgtcat gcagtgcaat acgtagacgc gtcgcgcaat tgttaatgct
    21961 ggagatattg ccgaccccgc ccagggcttg caggataccg gcagcctgat ccagctcttt
    22021 ttttggctct gcagcggtgg tttggcctcg cgaggctttg tattcggctt ttgagtagag
    22081 tttcacttcc gcatcttcac gtcccggcgt gcacatgttg aactgcagaa tcagggtgcg
    22141 gaaaaccacg aagtacagca gggtaaagca caacccaatg gcgatttggg tcagcatcat
    22201 atccgcgtgg ttgctgaaca tcgggatcca gttttgcggt aaaacctggt caatcagacc
    22261 tccgcccatg ttgcccacca caccaaagag atacattacg gtcgacattg aggccgccag
    22321 cacggcgtgt accgcaaaca gcaacggtga aatgaacagg aaggtaaatt ccagcggttc
    22381 ggtaattccc accagcatgg cggttaaggt tgcgggaatc agcaagcccg ccacttttac
    22441 ccgattttcc ggtgcggcag tgaagtacat cgctaaagaa atgcccacgg caccaaagat
    22501 ttttgagtta ccgtgcaggg caaaaccgcc ttccgggaac aacgatttca gtggctcggc
    22561 gctcagactg aactcttgca gatgctgcgc ccagtacatc tgaatgccgc cttcaacagc
    22621 tgccggacca aagataaacg gtccgtagat gaagtggtgt aaaccggttg ggatcagaat
    22681 acgttcgagg aaggtgtaaa cccacacccc aagcgcaccc gccgaacgca ggaacgcttg
    22741 cagagattca atccccattt gtacttttgg ccagccgagc aatgtcagcc aggcacaggg
    22801 gatcatcacc aggaaggcga taatcaccac ataagacgtc ccctggaaaa tgccgagaaa
    22861 aaccggcagt tttttatcga acagacggtt atgcagcgcc gtcacaatgc cggaaatgat
    22921 aattgcgccg ataatgctgg tatcgagggt tttaatcccg gccatcattg tcagaccgct
    22981 acctgccacc gcgtcctgag tgaaatcgac gccgaagtag cttccccagg tcattcccat
    23041 cgcattgatg aaatagttcc aggtcaggaa actcaccatc accgccagac aagcacgtcc
    23101 ctgcgcttgc ttagcaaggc caatgggtaa accgacagca aaaatcagcg gcatattacg
    23161 gaataccgtc caaccgccct cttcaataat gtgtacgatt tgcgcgaata aactgttcgg
    23221 atcggtcagt gattccccga caaacatcgg gttttgcagc aagatggcaa gacccaccac
    23281 aatcccggcg aagggaaaca gcagcactgg cgtgaacatc gcgccgccaa agcgttgaat
    23341 ttgactgagc atttccacat cctcttttct caattctgta agtaggaagc ctgattggtt
    23401 atgcggcaga cctggagcga ggataggaag atgcgatcgg cagaacatga cgacccaatg
    23461 ggattcgtga tcgctttcat gcttttcgtt gggcggtaga gatctacgtt ttcgcgaaaa
    23521 agtagacatc tgtgagcaca ggtacacagc atcgaacaca tctttaaaaa aaagattttt
    23581 ttcaatcgat taagcagaac ttgtgggcgc attacccgtg cttgcaggca aaaaagagag
    23641 ctagagatga tctacaaaag cattgcggag cggttaagaa ttcgacttaa ttccgcggat
    23701 ttcacgctaa acagccttct tcccggtgaa aaaaagctgg cggaagagtt tgcggtatcg
    23761 cggatgacta tccgtaaagc cattgacctg ctggtagcgt gggggctggt ggtccgccgc
    23821 cacggcagcg gcacttacct ggtgcgcaaa gatgtgctgc atcaaaccgc cagcctgacc
    23881 ggactggtgg aggtgttaaa acggcaggga aaaacggtca ccagccaggt gctgattttt
    23941 gaaatcatgc ctgcgcctcc ggccattgcc agccagttac ggattcaaat caacgagcag
    24001 atctacttct cccgtcgcgt tcgttttgtg gaagggaaac cgctaatgct ggaagacagc
    24061 tatatgccgg taaaactgtt ccgtaacctt tcgctgcaac atctggaagg gtcgaagttt
    24121 gaatatattg aacaagagtg cgggattttg attggcggta attatgaaag cctgatgccg
    24181 gtgctcgccg atagactgct ggcgcggcaa atgaaggtag cggaacacac gccactgctg
    24241 cggatcacct cgttgtcata tagcgagagc ggggagtttt tgaattattc agtgatgttc
    24301 agaaatgcca gcgaatacta ggtggagtac catttacggc gactccaccc ggaaaaaagt
    24361 taaccgatac tccagaagag caccgccagt aattgggggg tgataatgcg caggaacatc
    24421 actaacggat agacagtggc gtaagagagc gccgccgcac cactggttgg atgaagatta
    24481 ttagcaaacg ccagcgccgg aggatcggtc atggaacctg ccagcatccc gcacatggtc
    24541 aggtaattca ttttggctaa catccgcgcc agaatgccaa cagtaatcag cggaacggcg
    24601 gtgatcaggg caccataacc aatccagctt agcccttcgc cattgaccag ggtattcaca
    24661 aaatccccac cagatttcag accaacgacc gagaggaaca gcacgatccc cagctcccgc
    24721 agcacgaggt tggcacttgg cggcataaac cagtacagct tgccgatact gccgatacgc
    24781 ccgaggatca acgccataat cagcggaccg cccgccagcc ccagtttcaa cgcggccggg
    24841 aatcctggca caaagacggg aatagaacct aacaatacgc caagcccgat gccaataaac
    24901 actggcagca tctgaacctg ttgcagtttt tgctgcgcat tccccagcac attggcaacg
    24961 gcatcaattg cggacggacg ccccaccagg ttgaggatat cgccgaactg caggctgata
    25021 tcgccactgg cgaccagttc gaccccggca cggttcaggc gcgagataac aacgtcatag
    25081 cgttctttaa agtgcaggtc gcgaatacgt tttccgagca cgttttcatt ggtgaccacc
    25141 acacgctcga cgcgcaaatc agtgcctttc gtagacagcg aggtatcgac ctcctgacca
    25201 atcaccagtt gcgcattatg taaatccgct ggctgcccca ccagatgcag caaatcgccc
    25261 agttggataa tggtatctgg cgaaggtact tttagggttt cttctcgttt cagacgcgag
    25321 cagataattt tgtcgccgtt gagaatcggt acatctttaa tggctaaatc atgcaggtta
    25381 gggttctcaa cgcgaatatt gatagtcttg atcagcgcgc cgccattggt gcgtgaagac
    25441 tcgtgctgct gagcttctgt ctcgacattg acgcggaaaa taacccgcaa catccacatg
    25501 gtgaacaaaa tcccgcaaat gccgaatgga tacgccatcg cgtaactcat ccccatctga
    25561 tcgaccattt ccattggtgt acccaggtcg cgcaagatct gctgccctgc ccccagcgct
    25621 ggcgtattgg ttaccgcacc ggagaaaatc cccagcacta ccggcagtgg aatatcaaac
    25681 agtttatgca ggatggcggt aaccagacca ccgatgatga cgatcagaac agcaaacagg
    25741 ttgaggcgta atccggagac gcgcaatgag gcaaagaagc ccggccctac ctgaatcccg
    25801 atggtataaa cgaacaggat caggccaaat tcctgaataa catgcagcat atcgctactt
    25861 aatgtcatcc ccgcctgaga aacaaaatgg ccgacgatga tcccaccaaa cagcacgccg
    25921 ccaataccta atcctatgcc gcgaaatttg acgttgccga taaacaaacc gacgactgcc
    25981 accaaagcca gaatactgac cgttaatgct atatcactca tcgtccattt ctcttgagat
    26041 aacattttaa ttgctacgga ttatggctga atcggtgaag cagatttgcc ttatagcgca
    26101 cattatcacg ggaaagaacg tgccgaaata tcttaaacag tcgaaattta acgcattgtt
    26161 gaagaagaaa gacaaaaaaa gccccaccag aatggcgggg caaagagaat agctagttag
    26221 ctatttaacg cgggacgttc gctgatagcg atacgctgcg ctgcgatggg ttcaggctca
    26281 ttacgaatta aatcaatatg cagtaaaccg tttacgaagg ttgcgccaga gacttccata
    26341 ttttcagcca gcgtaaaact caggctaaat ggctgattca taagcccttg atgcagccat
    26401 tttttctctt cttttggctg ctccggcgtg ccttttacgc tcaggcgcgt accttccagt
    26461 tgaatctcta aatcttcctg acggaaacct gccagcgcaa gggtaatgcg gtagtggtta
    26521 tcgtcgcttt tctcaatgtt gtacggcggg aagctctggc tttcaccggc gttttgcagt
    26581 gcgttggcca gtttgtcaaa accgatccat tgacgcatca gtggggataa atcgaagtta
    26641 cgcatagtca tttctccttc taagaagcga gtaagtacct gcaaatccga agattcgcat
    26701 atgctccctg acggcgagca tggagatgtc aggccgcgcc aggcggcctt agggaattag
    26761 ttgatttcga tacggcgcgg ttttttcgct tccggaatca cgcgttcgag atcgatatac
    26821 agcaaaccat ttaccaggtt agccccacga acatgaatgt tctcagctaa ctggaatttg
    26881 cgttcaaagt tgcgttcagc gatgccctgg tacagatagg tgcgctcttt ttgttcgtcg
    26941 gcgtgagcac ctttcaccac cagcagatta tcctgggcgg taatttccag ttcgctctca
    27001 gcaaaaccag ccacagcgat agcaatgcgg taatggtttt cgtctaccag ttcaacgtta
    27061 tacggagggt agccgccatt actctggctc tggttgtttt ctaagtggtt aaacaaacgg
    27121 tcaaatccaa tagcagaacg gtaaagcggg gataaatcaa agttacgcat aatcaatagc
    27181 tcctgaaatc agcgagaatg taagaccttc cacaatggac aggtcaggta gccagaacac
    27241 ccatcaggcg cgtcctcatc gactacgatg taaaatgggt ctggaaatga acttttcaag
    27301 ccttatcagc ggactttttt ttgcagttta tggtctattg catagactga gggggcagca
    27361 cgaaacgtta aagtgcgata gccgccacag tgcgacgtaa tgatgatgtt attttcagca
    27421 agggatcgct ataactcatc aggccaacac ccacgatcaa cagatgaatt tattatgata
    27481 agaaatgtgt tgttagcgtt catgatatgc agcggaatga cattactcgg aggatgctcc
    27541 agcgtgatgt cccataccgg ggggaaagaa ggaacgtatc ccggcacccg cgccagcgcc
    27601 accatgattg gcgacgatga aaccaactgg ggcactaagt cgctggcgat tctcgatatg
    27661 ccgtttaccg ctgtaatgga cacgctttta ctgccgtggg atgtgttccg caaagacagc
    27721 tcggtgcgct cgcgcgttga aaaaagcgaa gccaacgcgc aagccaccaa tgcggttatt
    27781 ccaccggcca gaatgcctga taactgattt agcgatcagt ttcggtgatc cacagctggc
    27841 caccttccat ccacgccagc cattgaccat ccggcgagaa gaccacggcg tccgcagaag
    27901 gtggattggc gtggttttcg gttaaatact caacctcgcc gctttgcgca tgggcacaaa
    27961 caattcgatt atccagcaca aagcccaacc attctcctga cggatgccag ttaaatgcag
    28021 actgaatatc cgttttgtta tgggttaact tgcgcggttc gccgccctgt ggcgagataa
    28081 gccacagttg cacaatgccg ttatcatcac gcattaaaaa cgcgatttgc gtaccctgcg
    28141 gattacagcg cacccagtgg cgcgggacgt tgactaaccc cggataagcc cgatgatggg
    28201 taaaggttaa acgtcgctgc acgacgccac gcggtggcgc gggtagggtt gtttccgttc
    28261 cacttaacgg cgcatcacct gccgatttcc agctagcttc atcttgcggt aactcaacga
    28321 taaatagctc cggcactttc tcgccctttg gcgaaagtgt gtcgccaata aacgccagcg
    28381 cgtgatttcc tacccatcct tcttcataag cacgattgat ttcatcactg ccaggctgcg
    28441 gcgtgggcgt ggttttactc accagcacac accagtggct accgctgtat tcacgcggat
    28501 gttgcttttg tacgttgacc gggccaaacg gtgcagcaac cccgacgttt cgcaaattca
    28561 gcgccggatc gagttgatgc attacatggt cgttataggt aaagctcccc ctttcaccgt
    28621 tcgggctaaa gacatgcaca tggctgccac cgcgcagcgc tcctggcgta tacggcgcgg
    28681 taatatccat tgcatcgaga ttgctcacct tgccgccttc agcaatgact ccgcgtcgat
    28741 gatggaagtc gtaatgccat gtttcatcag gattttcagg gccgtgaata aagacatatt
    28801 tctctgactt cggatgaacg gtcaccacgc cgacgtgtgc gccctgtgac gcgcgataga
    28861 taacctcgac ctcgcctgta tggatattca cacgctcaat ggtctcgccg gtaaacgacg
    28921 ccccagaagg acgcacgtca aataccagcc actggctgtc gggcgtccag gtattggtat
    28981 tggtattggt attggtattg gtattggtat tggtattggt attggtattg gtgagcaggt
    29041 gattacgggg agcaaaggtt atctgtttca tggcgcggtc ctgatacaaa acggttcccg
    29101 ccatcatact tcacaaggac ttcactttca gacaatagtc ttttgccatc gtgatgaatg
    29161 tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg
    29221 cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt
    29281 gcggtactga aggtttcagc aaaccttgcg gcggtctgct ggcaccggac gcgcagcgat
    29341 cttttattcg cgatggactg acgcttcctg tcgatgtgat cgccaatcca cagattttca
    29401 gcgtcaaaac tgtcgacgtc gccgcatcgc tcacgcgtaa ctaccagcga agctatatca
    29461 atattaatcg ccatgctttc gacttgtgga tgaaatcgct gatccccgcc agcgttgagg
    29521 tttatcacga tagcctgtgc cgaaaaatct ggcgtgagga tgataaatgg catgtcattt
    29581 ttcgtgcaga cggctgggag caacatatta ccgcccgcta tctggtcggt gccgatggcg
    29641 caaactcgat ggtgcggcga catctctacc cggatcatca aatccgtaaa tatgtcgcta
    29701 tccagcagtg gttcgcagag aaacatccgg tgccgttcta ctcctgcatc tttgataatg
    29761 cgataactga ctgttactca tggagtatca gcaaagatgg ttattttatc tttggcggtg
    29821 cctatccaat gaaagacggt cagacgcgtt tcacgacgct gaaagagaaa atgagcgcct
    29881 ttcagttcca gtttggcaaa gcggtgaaaa gtgagaaatg cacggtgttg tttccctcac
    29941 gctggcagga ttttgtctgt ggtaaggaca acgcctttct gattggtgaa gcggcgggat
    30001 ttatcagcgc cagctcgctg gaggggatta gctatgcgct ggatagcgca gagattctgc
    30061 gtgcggtgtt cctgaaacag ccggagaaga gcaacgccgc ttactggcgc gccacacaca
    30121 aactgcgttt aaaactcttc ggcaagatag taaaaagccg atgcctgacc gcaccggctt
    30181 taagaaagtg gattatgcgc agtggtgtgg cgcatattcc acagttgaaa gattatccaa
    30241 cgcgcttcac atcacccacc agcaggatgt aagagagcgc gccaatcaac gcgacggcgg
    30301 agatataaac cagtgcaggc gcgaaaccgt aaccctgcgc caggtacccc accaccagcg
    30361 gaacggtgat gccgcccaga ccaccggcga agttaaacac gccgccggtt aaaccaatca
    30421 ggcgcatcgg tgccagagaa gagaccagcg accaggtaat cgaagcaaaa ccgttgccga
    30481 agaatgccag cgccatcagg cacataatca tcatcggatc gttggtgtag ttagcgccca
    30541 taatgcaggt ggagatcagc aagccgcaga taatcggcgt tttacgcgca aagcccagtg
    30601 aaaagccctt acgtaccagc agatccgcga cccagccaga gagcaggacg ccgacaaacg
    30661 ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag cgccgtgatt cctttttcct
    30721 gggttaaata gttcgggaac caggttaaga aaaaccacag tgcagaagcc accgcaaatt
    30781 gcccaagata aacgccgatc agtttacgat ggaacaccag tttccagtct ttggctgtta
    30841 acggctgacg cgcctctttc ttcaccggcg catcaccatc caccagaccg ccgccatcac
    30901 gaatgtaatc cagttcagct ttgctgatac ctttggtcag gcgcggcggc tgataaacct
    30961 taaaccaaat cagcgaccag ataatgccga taccaccagt gacaatgaac acccagtgcc
    31021 agctcaacat ctcctgaatc cagatcagca gcggagtcaa aaacgccaga ccgacaaact
    31081 gaccagacgt ataaaaacca acggcggaag cgcgttcatg ttccgggaac cagctggtca
    31141 ccatccggtt attggtcggg aaagcgggcg cttcgaaaat accggttatc gcacgcagac
    31201 caattaatga cattaatccc gtggcaaagc cttggaataa agtcaccact gaccaaccaa
    31261 atatcgcaat aaaataagtc acgcgagaac ctacgcgatc taaaaaccaa ccgccgggga
    31321 tctggcatag cgtataaagc caggcgaagg ccgaaaatac atagcccatt tccgctttcg
    31381 taatgccgaa ctcttcctga atatgggcgg aagccacggc caggttagcg cggtcaacat
    31441 aacaaatgac caccgtaata aagatcatca ccagcgtcag ataacgccga cgccccggct
    31501 ttgctgcatt aacgggaata tccatagcga gctttctcca gattttgggc atagcgaagc
    31561 cgctcaccat gccctgtaat ttacagaggg ttatattttt gtattgctgt tttagtgccc
    31621 gatgaggggc ttacgtggca ggaattacca ctctgctacg ctattatctt catgacgcca
    31681 gagcggatta cgccagtccg gggcattttt actgaactca ataactttag cttcgtcgat
    31741 ttccacacct aagcccggtt tcgttaacgg tttaaagaag ccgccaacca tgctgaagtc
    31801 ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg ttgtaatgaa tgcccatact
    31861 ttgttactga agtaccgcgt tataggaaac aaagtcgata tgcaggcaag ccgccagtgc
    31921 aatcggtccg aacggacagt gcggcgcaag ggtcacatca taggcttctg ccattccagc
    31981 aattttgtag cattcggtaa taccgcctgc atgggagaga tccggttgca gaatcgaaat
    32041 accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag aacatgcgct cacccgccgc
    32101 cagtggaata tgcgtttgtg ccgccaattt cgggtagtat tcggcttgtt ccgccagcac
    32161 cggctcttca ataaacagcg ggcgatacgg ctccagttct ttaatcagca ctttcgccat
    32221 tggcgcgctg acgcgaccgt ggaaatcaag accaaactca atctgattgc caaaagcttc
    32281 acgaatttgt gccacggtgt tgactgccgc atctaccgcg cgggagttat caattagccc
    32341 cagttcttca caaccgttca gtttgaaggt atcgaagccg atttcgcgca gggttttaat
    32401 gccgtcgata acatccgccg gacgatcgcc gccgacccaa ctgtaggctt taattttgtc
    32461 gcgaaccagg ccgcccatca gttgccagac tggcgcattc agcactttgc ctttgatatc
    32521 ccataacgcc tggtcgatcc cggcgatggc gctcatcagg attggaccgc cacgataaaa
    32581 tccggcgcga tacatcactt gccataagtc gttgatgcgc gaaggatcct gaccaatcaa
    32641 atagtcaccc agctcgtgaa ctgccgcttc caccgtacgg gcgcggcctt caatcacggg
    32701 ctcgccccaa ccgaccacgc cttcatcggt ttcaattttc aggaacatcc agcggggagg
    32761 taaacgatac gtggtaattt tggtgatttt cattgcactg cctctcgata cgccttaaca
    32821 aatgctgctg cctgctgcgc ggtgcgttct acggactgcc cggcgcgata aagatcgctg
    32881 cctaagcccg ccccaacaca acctgcgtct atccactgcg ccaggttttc tggcgtcacg
    32941 ccgccaacgg caaagactgc gatgtccggt ggtaataccg cttttaacgc tttgatgtat
    33001 tgcggaccaa aagccgatga cggaaatatt ttcagcgcct gcgcgcccgc ttcgagcgcg
    33061 gtaaaggctt cggtcgccgt cgcgcagccg gggcaaacgg tcatgccgta gcccaccgcg
    33121 cggcggatca cttcactatg gatattgggc gtaacgatga gctgacagcc catcctggcg
    33181 agcgcatcga cctgttcagg tttcagtacc gtacctgcgc caatcaacgc cttgtcgcca
    33241 tacgcatcaa cgatggcggg aatgctctgc tcccattgtg gggaattcag cgggatttca
    33301 accgcgtcga acccggcgtc aatcaccgcg ccaacatgcg ccagcgcctc gtcgggcgta
    33361 atgccgcgca aaatggcgat cagcgggagt ttagtttgcc actgcatgag cgatgctcct
    33421 tataccagcc tgaaatgccg tgtcgcccgc caccgccgcc acgtcgcaac ccatcgcctg
    33481 aaaggcttgc tggtagcgcg cggtcagcga tgttccggcg acaagggtga tggcgtgttg
    33541 atgggtcaca tagtcgcgca tactggcaac ctctgcgcca atcaacaaac cagagagaaa
    33601 ttcgctgacc tgttcgcggg gaagtgttcc cagcacatgc gaggcgcgaa cttcaaaaag
    33661 ctgcggcaat atggcgggcg cattaaggcc gcgctcaagg ccagccgcga aggcatcggc
    33721 agagttttcc tgcggcggca aacctgcgcc aatcaatgag tgatttaaca gtaaatgatg
    33781 taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc tggctatcgg cctgcaccca
    33841 tttgcaatgc gtaccgggca taacataaag agaggaagga gccagagctc gcgcgccgat
    33901 caattgtgtt tcttcgccgc gcatcacatt gtggttatcg tcatgagaga cgcataatcc
    33961 gggaataatc cagatattgt cgccaacaga cgttaattgt tcgccaatag acgaaaaacg
    34021 ggcaggaaca gataaatacg gagcaacttt ccagccgaca ttgctgccga ccattcctgc
    34081 catcaccact ggcgtatttt cttcacgcca gtcggtcgtg acttctgcta acaccgcagc
    34141 cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat tgcctgctct ccaggcagtg
    34201 gtcgccctga taaagccagg cgcgcagatt ggtcgatccc cagtcaattg cgatgtagcg
    34261 agctgtcatg tgatttcctt taaccttcgt gtcgagctgg caatcatggt aagcgccgcc
    34321 tgctctgccg catcgccgtc ctgatgccgt atcgcatcga acagcgcctt atgttcctgg
    34381 agcgtttgcg gcatgttggc ctcatcgccc atccaggttc gttcaaaaac cgcccgctgc
    34441 agcgaactga tcgcaatgct aagttgctgt aacaccgggt tatgcaccga ctgcagcacc
    34501 gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc ggtcctgatt gttggcaatc
    34561 atctcgttca gcgccgattc aatctgcgcc agatcgctgg aggtcgcgcg ctctgctgcc
    34621 caacgggcaa tcgccggttc caccagattt cgcacttcgc tcatggcact gataagccgt
    34681 gggtcgtagt cgttttccag cacccattgc agtacgtcag tgtcgaggta attccactgg
    34741 ttacgcggtg ccacaaacgc cccgcgataa cgtttcattt caatcagccg cttcgccatc
    34801 agcgaacgga acacctcacg gatgatgttg cgcgaggttg caaactcttc acagagctcc
    34861 gcctcagccg gaagtggcga gccaggtacg tatttgccgt gaacgatctg tttacccagc
    34921 gtaatgacaa tgcgatcggt tttattgaga gtcatggaga gtccttgtgc tctgtatgtt
    34981 cttctctact ttaccccgat cgatgcataa cgcggcaact ttgtagtacc agcgtgatga
    35041 cgttcgcgtt tgccgcgcgc gtaatgtagt acaaacttat attgttgtgc tacaatttag
    35101 atcaaaaaaa gaacaataca taaaaaaatg acatgcgtcg ggcagaaatc tgaaaaggga
    35161 tatcaggcgc taaacaggag ggaaagaaga gtatgctttc aacggcttag ctactcgttt
    35221 aaaggattaa tcatgaagtt gaattttaag ggatttttta aggctgccgg tttattccca
    35281 ctggcgctga tgctttcagg ctgtatctcg tatgctctgg tttcccatac cgcaaagggt
    35341 tgttcaggaa agtatcaatc gcagtcagac accatcactg ggctatcgca ggcaaaagat
    35401 agtaatggaa caaaaggcta tgtttttgta ggggaatcgc tggattacct tatcactgat
    35461 ggtgccgatg acatcgttaa gatgctcaat gatccagcac ttaaccggca caatattcag
    35521 gttgccgatg acgcaagatt tgttttaaat gcggggaaaa agaaatttac cggcacaata
    35581 tcgctttact actactggaa taacgaagaa gaaaaggcac tggcaacgca ttatggtttt
    35641 gcctgtggtg ttcaacactg taccaggtca ctggaaaacc taaaaggcac aatccatgag
    35701 aaaaataaaa acatggatta ctcaaaggtg atggcgttct accatccatt taaagtgcga
    35761 ttttatgaat actattcacc cagaggcatt ccggatggtg tttccgcagc attactgcca
    35821 gtgactgtta cactggacat cattactgca ccgctgcaat ttctggttgt atatgcagta
    35881 aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt tgcccgccca cagattaatt
    35941 cagcacatac ttctcaatag caaacgccac gccatcttca aggttggatt ttgtgacaaa
    36001 gttcgccact tctttcaccg aaggaatagc gttatccatc gccacaccga cgcctgcata
    36061 ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc atgatttctt ccggtttaat
    36121 acctaacacg tcggccagtg atttcacacc cgtacctttg ttaacgcgtt tatcgaggat
    36181 ttcgaggaag tacggcgcac ttttcagcac ggtatatttc tctttcactt cctgcggaat
    36241 acgcgcgata gcctggtcga ggatggcggg ttcatcaatc atcatcactt tcaggaactg
    36301 ggtattgggg tccattttct ccgcttcgca gaacaccagc ggaattgtgg caacgaagga
    36361 ttcatgcacc gtgtagtagc tgatatcgcg gttagcggtg tacagcgtgg tgcggtccag
    36421 ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc aggaaacgat agtcgtcata
    36481 gctgagagcg gtttgcgcca cggtgctacc atcagcggcc ttctgtacca gcgcgccgtt
    36541 ataagtaatg cagtagtcgc ccggctgttc catatgcagc tctttcaggt agttgtgcac
    36601 acctgcatac ggacgacccg tcgttagcac gacattcacg ccacgggcgc gagctgcggc
    36661 aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc agcagaaggg tgccatccat
    36721 atcgatagca atgagtttga tagccatgag ttcccaaggt agattagttc ctgacccatg
    36781 ctaacgcgat tccgctcaaa aatcagtaca acacccgagg gaaaagggga tgcaacgcgg
    36841 gttgcatgct ccccatttgc ttagcggaag agtttccctt tcagcagttc catgcctgcg
    36901 gaaagcagat cgttattggc ttgtgctgac acttcacctt gcggtgagag cgcatcaata
    36961 atcttcggca attgctctgc cagtaaactg gaagccgtac tggtatccac gccaagtttt
    37021 tgcccgagat cggacaccgc atttgtgccg agcgccgact ccagttgctc gccgctaact
    37081 gattgatttc tctgttgatt actcaaccag gttgagagaa tggcccctaa gccgccactt
    37141 tgcagttttt ccagcagcac ctgaatgcca ccctgctcct caacccaact taaaatcgcc
    37201 tgatatttcc ccgcatcgcc tttcagaaag gcaccgacaa cttcatcaaa aagccccatg
    37261 ataatcacct gtaaagcgtt acgtgttgac ccaaaaagta tagatctgcg ggatgataat
    37321 tgcggattgc agaaataaaa agggcggaga tgatctccgc ccttttctta tagcttcttg
    37381 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaatca tgcaaaatca atatattgca
    37441 ggagtctcgt aggactaata agcgtagcgc atcaggcacg ctcgcatggt taacgccatt
    37501 aaatatcaat attcgccgct ttcagggcgt tctcttcaat aaacgcacga cgcggttcta
    37561 cggcgtcgcc catcagcgtg gtgaacaact ggtcagcggc aatcgcatcc ttaacggtaa
    37621 cgcgcagcat acgacggctt tccgggtcca tggtggtttc ccacagctgt tccgggttca
    37681 tctcgcccag acctttataa cgctggatgg agaggccacg acgggactct ttcaccagcc
    37741 agtccagcgc ctgctcgaag ctggctaccg gctgacgacg ctcgccacgt tcgataaacg
    37801 catcttcttc cagcaagcca cgcagtttct cacccagcgt gcagatacga cgatattcgc
    37861 caccggtgat aaactcgtga tccagcggat agtcagtatc cacaccgtgg gtacgcacgc
    37921 gaacaatcgg ctcgaacagg ttttgttcgg cattggtgtg gacatcaaac ttccactggc
    37981 tgccgtgctg ttctttgtcg ttcagttcgc tgaccagcgc gttcacccag cgggtaacgg
    38041 tctgctcatc agaaaggtca gcttccgtca acgtcggctg atagataagc tctttcagca
    38101 ttgctttcgg ataacgacgc tccatgcggt tgatcatttt ctgcgtcgcg ttgtactcag
    38161 ataccagttt ctctaacgct tcgccagcca atgccggtgc actggcgttg gtgtgcagcg
    38221 ttgcgccgtc cagcgcgata gagatctggt actgatccat cgcttcgtcg tctttaatgt
    38281 actgttcctg cttgcctttc ttcactttgt acagcggcgg ctgagcgatg tagacgtgac
    38341 cgcgttcaac gatttccggc atctgacgat agaagaaggt caacagcagc gtacgaatgt
    38401 gcgagccgtc gacgtccgca tcggtcatga tgatgatgct gtgataacgc agtttgtccg
    38461 ggttgtactc gtcacgaccg ataccacagc caagcgcggt gataagcgtc gccacttcct
    38521 gagaagagag catcttatcg aagcgcgctt tctcgacgtt aaggatttta cccttcagcg
    38581 gcagaatcgc ctggttcttg cggttacgcc cctgcttcgc agagccgccc gcggagtccc
    38641 cttccaccag gtacagttcg gaaagcgccg gatcgcgttc ctggcagtct gccagtttgc
    38701 ccggcaggcc agctaagtcg agcgcacctt tacggcgggt catttcacgc gcgcgacgcg
    38761 ccgcttcacg ggcacgggca gcatcgataa ttttgccaac cacgattttc gcatcagttg
    38821 ggttttccag caggtattcc gccagcagtt cgttcatctg ctgttcaacc gccgatttca
    38881 cctcagaaga aaccagtttg tctttggtct gggaggagaa tttcgggtcc ggcactttta
    38941 cggaaacgac cgcaatcagg ccttcacgcg catcgtcacc ggtggcgctg actttggctt
    39001 ttttgctgta gccttctttg tccatgtagg cattcagggt acgggtcatc gccgcacgga
    39061 agcctgccag gtgagtaccg ccgtcacgct gcggaatgtt gttggtaaag cagtagatgt
    39121 tttcctggaa gccatcgttc cactgcagcg ccacttcaac gccaataccg tctttttcgg
    39181 tggagaagta gaagatattt gggtggatcg gcgttttgtt cttgttcaga tattcaacga
    39241 acgctttgat gccgccttca tagtggaagt ggtcttcttt gccgtcacgc ttgtcgcgca
    39301 gacgaatgga aacgccggag ttgaggaacg acaactcacg cagacgtttc gccagaattt
    39361 catattcgaa ctcggtcaca ttggtgaagg tttcgaggct gggccagaaa cgcaccatgg
    39421 tgccggtttt ttcagtctcg ccggtaaccg ccagcggagc ctgcggtaca ccgtgttcgt
    39481 agatctgacg gtgaatttta ccctcgcgct gaataaccag ctccagtttt tgcgacaggg
    39541 cgtttactac cgaaacacca acgccgtgca gaccgccgga cactttatag gagttatcgt
    39601 caaatttacc gcctgcgtgc agaacggtca tgatcacttc cgccgccgat acgccctctt
    39661 ccgggtgaat accggtcgga atgccgcgcc cgtcatcctg tacagagaca gagttatcgg
    39721 cgtgaatggt gacgataatt tctttacagt gacccgcgag cgcttcgtcg atagcgttat
    39781 ctaccacctc gaataccatg tggtgcagac cggtgccgtc atccgtgtcg ccgatataca
    39841 tacccgggcg cttacgcacc gcatccagcc ctttcaggac tttgatactg gaggagtcat
    39901 aagaattcga catcaacgtt tctcgctcat ttatacttgg gttaatctgt tattttaccc
    39961 ttttccacgg taaacatctt cgaattttcg tccgacatgt ctataacgtg ttcagcactg
    40021 atcgcgctga caaagacctg tgattgcgtc gcttttaagc ggctggcaag caacccgcga
    40081 cgctcatcat caagctcaga ggcaaaatca tctatcaggt agagacaccg ccgcccgctt
    40141 tcacgagtga ggaactctcc ttgcgccaga cgtaaggcgc acatcaacag cttaagctgc
    40201 ccacgcgata aggtatcttc caccggcgca ccgtcggcgc gaatgcgtaa gtccgcttta
    40261 tgcgggccat gcgcggtgta ggttagctgg cgatcgcgtt caaaattacg ttccagcacc
    40321 tcagcgtact ctgtctcttt ctcccagccg cgctggaaag agaaagtcag agaaaactca
    40381 gggagaaatt gcttacaggt atcatccata tcggccgcga taccggcgct atactccgcg
    40441 cgccaggtgc tgatttgctc cgccagcggg atcagttctt tatcccacgg gcgtagctgt
    40501 tcgtaacgag tcacctggcg cagcgccgca ttgcgctgct tgagcaatcg cttgagattg
    40561 ctccaggcgg tgaaaaatcc aggttcgttg tgaaagcatc cccagtcgag gaatgctctt
    40621 ctgtatttgg ggccgccgtt gagtaaagta aacccttctg gcgttatcaa ctgcatcggc
    40681 atcaggtgcg ccagttccgc gactttatgc ccgtccgtac cgtcaatacg gactttgctg
    40741 tcgccctgtt tgtctttggt taagccaatc gccgtctcgc gctcttcgcc ctgtaatcgc
    40801 ccgtggagaa caaatgcctc ctgctcatgg cgaatgacgc gaccaatctg caaactgcga
    40861 aacgcccgac catggccgag cgtatagatg gcttccagca cgctggtttt gccactgccg
    40921 ttggcaccta ccagaaagtt aaagccggga gataaagcga gatccgcggt ttcaatattg
    40981 cggaaatcgc ggatcaacaa gcgggtgagg gacattacag tctcattggc atgacaacat
    41041 aggcagccga ctgtgatgcg gcatcttcaa tctgcacgct ggaaaccgaa tcggtcagca
    41101 tcatgcggac gttttcgcat ttcagcgcgt tcagaacatc cagcacatag ctgacgttga
    41161 agccgatttc catctccgca ccgctatagg taacgtcgag gatctcttcc gcttcttcct
    41221 gttccgggtt gttggcagta attttcagct ggttttcgct gacatacagg cgcacaccgc
    41281 ggaatttctc gttagagaga atcgccgcgc gagcaaacgc ctgcttgagc agatcgcagc
    41341 ctgcttccag atgtttgtcc gggttcttcg gcagaacgcg acggtaatcc gggaagcgac
    41401 catccaccag tttcgaggtg aaaataaagt cgccaacgtg ggcacgaatg ttattgctgc
    41461 caatctgcac acgcagcaga ttgtcgccgc cgtcgagcat acgcatcagt tcaatcacgc
    41521 ctttacgcgg tacgatcacc gaatggcttg gcaaagattg accaattggc attgaacaga
    41581 ccgccagacg gtggccgtcg gttgccacgg tgcgcagttc ttcaccttcg gtttcaaaca
    41641 gcataccgtt taagtaatag cgaacatcct gatgcgccat cgaaaactgg gttgcttcaa
    41701 tcagacgctt catcgtcgcc tgcggcaggg taaattcgac ttcgctctgc cagtcatcga
    41761 ggttcgggaa atccgccgct ggcagggtag acagcgaaaa acggctacgc ccggagcgca
    41821 ccagcatccg ttcaccttcc agctgcacgg caatttccgc gccttcaggc agaccacggc
    41881 agatatcaaa gaatttgcgc gccggaacgg tcgtcgctcc tggctcgtgt ggctgaacca
    41941 gcgcaacgcg tgccaccatt tccatctcga gatcagtacc ggtcagcgac aacgtaccgt
    42001 cagcaacctg taacagcaga ttaccgagaa tcggtagcgt aggacgacca cctaacggac
    42061 cgctcacctg ttgtagcggt tttaataaat gctcacgttc tacggtaaat ttcataggtt
    42121 tacgatgaca atgttctgat taaatttgaa aaatcttctt tgatatcgtg gctctcttca
    42181 cgcaactgct cgatcttacg gcaggcatga agcaccgtcg tgtggtcacg cccaccaaac
    42241 gcatcgccaa tctctggcag actgtggtta gtcagctctt tcgctagcgc catcgccatc
    42301 tggcgcggac gcgccaccga gcgggatcga cgcttggaaa ggagatccgc gactttgatc
    42361 ttgtagtact ccgccaccgt cttctgaata ttgtcgatgg tgaccagttt ttcctgcaat
    42421 gccagcaagt cgcgcagcgc ctcacgcacg aagtcgatgg tgatcgcccg tccggtaaag
    42481 ttggcgttag caataacgcg gttcagcgcc ccttccagct cacgtacgtt agatcgtaga
    42541 cgcttggcga taaagaacgc cacttcaccc ggcaaacgaa tgtcattttc gtcggccttt
    42601 ttcatcagga tcgccacacg ggtttccagc tctggcggtt cgatcgccac agtcagtccc
    42661 caaccgaagc gggatttcaa acgatcctca acgccgttga tctctttcgg atagcgatcc
    42721 gaggtgagaa tgatctgttg attaccttcc agcagggcgt tgaaggtgtg gaaaaactct
    42781 tcctgagatc gttctttatt agcaaaaaac tgaatatcgt cgatcagcag tgcatctacg
    42841 gaacggtagt agcgtttaaa ctcttcgatc gcgttgtttt gcagggcttt aaccatgtcc
    42901 tgaacaaagc gctcggagtg catataaacc actttggcat tcggcttgcg cgccataatg
    42961 ccgttaccca ccgcatgcag caggtgagtt ttacccagac ccgtgccgcc ataaaggaac
    43021 aacgggttat aggcaccgcc agggttatcc gccacctggc aagccgccgc gcgcgccagt
    43081 tggttagatt taccttcaac gaagttatca aacgtgtgtt tgacgtttac gttagaacga
    43141 taggtcggtt ctgccggggc cgggacgtta tcccaacctg agcgcgtaga aggcgcagca
    43201 cgttgcggct gcgtttgcgc cacctgtgca ggggccgcga cgttgctcgt cactgccgct
    43261 tgtggcgttt gcgtcaccgg tttggtgccg acttcaaaac gcagctgtgg ggcatccgct
    43321 ccgcagaaac tggttagcag tccattgata ttattaaggt acttgtcccg tacccaatcg
    43381 aggacaaaac ggtttggcgc gtacagggcc agcgtgttat cgctcagttc cgcctgcaat
    43441 gggcgtatcc acatactgaa ttctgtggct ggtaactcat cctgcaatcg ggcaagacac
    43501 tgctgccaaa gcgaaagtga cacggcggac tccactcgaa caaaagtcga taatgactaa
    43561 ggctgaaaca ttcatgattg ttgacgtacg tcgaaaagac cctgcttgag ggtgacgcac
    43621 gaaccgctgt ctgcggttat atgcccgatc aagatcctgc aaaacgatcg ggaccgcgga
    43681 tcatagccta aactgcgcaa gagatcttct gtttctcaca gattttccct atttatccac
    43741 aggactttcc agaactcgct aagtgtaaac gatcctgccg cgaggcgggc acgatttacg
    43801 ccgcatattg gaaaatttaa tgaccataga caaaaattgg cttaatcgat ctaataaaga
    43861 tccaggacga tccttgcgct ttacccatca gcccgtataa tcctccaccc ggcgcgccat
    43921 gctggtttcc actggtgtga ggtcgtacat tttccctgcg aaaaggtgcg gaaaagcgcg
    43981 gtaaataagg aaagagaatt gactccggtg tgtacaatta ttacaatccg gcctctttaa
    44041 tcacccatgg cttcggtgtc catcgtttca tttttcggcg gatatccaat aaagccattg
    44101 aatttattca agtttaggta gaaatcgcca tgaaacgcac ttttcaaccg tctgtactga
    44161 agcgcaaccg ttctcacggc ttccgtgctc gtatggctac taaaaatggt cgtcaggttc
    44221 tggcacgtcg tcgtgctaaa ggccgcgctc gtctgaccgt ttctaagtaa taaagctaac
    44281 ccctgagtgg ttaagctcgc atttcccagg gagttacgct tgttaactcc cagtcaattc
    44341 acattcgtct tccagcagcc acaacgggct ggcacgccgc aaattaccat tctcggccgc
    44401 ctgaattcgc tggggcatcc ccgtatcggt cttacagtcg ccaagaaaaa cgttcgacgc
    44461 gcccatgaac gcaatcggat taaacgcctg acgcgtgaaa gcttccgtct gcgccaacat
    44521 gaactcccgg ctatggattt cgtggtggtg gcgaaaaaag gggttgccga cctcgataac
    44581 cgtgctctct cggaagcgtt ggaaaaatta tggcgccgcc actgtcgcct ggctcgcggg
    44641 tcctgatagc cctcattcgg gtctatcaac gcctgattag tccgctactc gggccgcatt
    44701 gtcgtttcac tccaacctgt tcaagctacg gaattgaggc attgcgcagg tttggagtga
    44761 taaaaggcag ttggttgacg gtgaaacgcg tattaaaatg ccacccttta caccctggtg
    44821 gtgacgatcc cgtcccgccc ggaccatttg ataccagaga acactaacga tggattcgca
    44881 acgcaatctt ttagtcatcg ctttgctgtt cgtgtctttc atgatctggc aagcctggga
    44941 gcaggataaa aacccgcaac ctcaggccca acagaccacg cagacaacga ccaccgcagc
    45001 gggtagcgcc gccgaccagg gcgtaccggc cagtggccag gggaaactga tctcggttaa
    45061 gaccgacgtg cttgatctga ccatcaacac ccgtggtggt gatgttgagc aagctctgct
    45121 gcctgcttac ccgaaagagc tgaactctac ccagccgttc cagctgttgg aaacttcacc
    45181 gcagtttatt tatcaggcac agagcggtct gaccggtcgt gatggcccgg ataacccggc
    45241 taacggcccg cgtccgctgt ataacgttga aaaagacgct tatgtgctgg ctgaaggtca
    45301 aaacgaactg caggtgccga tgacgtatac cgacgcggca ggcaacacgt ttaccaaaac
    45361 gtttgtcctg aaacgtggtg attacgctgt caacgtcaac tacaacgtgc agaacgctgg
    45421 cgagaaaccg ctggaaatct cctcgtttgg tcagttgaag caatccatca ctctgccacc
    45481 gcatctcgat accggaagca gcaacttcgc actgcacacc ttccgtggcg cggcgtactc
    45541 cacgcctgac gagaagtatg agaaatacaa gttcgatacc attgccgata acgaaaacct
    45601 gaacatctct tcgaaaggtg gttgggtggc gatgctgcaa cagtatttcg cgacggcgtg
    45661 gatcccgcat aacgacggta ccaacaactt ctataccgct aatctgggta acggcatcgt
    45721 cgctatcggc tataaatctc agccggtact ggttcagcct ggtcagactg gcgcgatgaa
    45781 cagcaccctg tgggttggcc cggaaatcca ggacaaaatg gcagctgttg ctccgcacct
    45841 ggatctgacc gttgattacg gttggttgtg gttcatctct cagccgctgt tcaaactgct
    45901 gaaatggatc catagctttg tgggtaactg gggcttctcc attatcatca tcacctttat
    45961 cgttcgtggc atcatgtacc cgctgaccaa agcgcagtac acctccatgg cgaagatgcg
    46021 tatgctgcag ccgaagattc aggcaatgcg tgagcgtctg ggcgatgaca aacagcgtat
    46081 cagtcaggaa atgatggcgc tgtacaaagc tgagaaggtt aacccgctgg gcggctgctt
    46141 cccgctgctg atccagatgc caatcttcct ggcgttgtac tacatgctga tgggttccgt
    46201 tgaactgcgt caggcaccgt ttgcactgtg gatccacgac ctgtcggcac aggacccgta
    46261 ctacatcctg ccgatcctga tgggcgtaac gatgttcttc attcagaaga tgtcgccgac
    46321 cactgtgacc gacccgatgc agcagaagat catgaccttt atgccggtca tcttcaccgt
    46381 gttcttcctg tggttcccgt caggtctggt actgtactat atcgtcagca acctggtaac
    46441 cattattcag cagcagctga tttaccgtgg tctggaaaaa cgtggcctgc atagccgcga
    46501 gaagaaaaaa tcctgattcg gtgagttttc gctaaaataa gggcggtcag ttgaccgcct
    46561 tttttctttt cgtagggcgg ataagcaccg cgcatccgcc acacaaagca acaggaacat
    46621 catgagcgat aatgacacta tcgtagccca ggccacgcct ccgggacgtg gcggcgttgg
    46681 catcctgcgc atctccggct tcaaagcccg tgaagttgcc gaaaccgtgc tgggtaaact
    46741 gcctaagccg cgctacgccg attatcttcc gtttaaagac gccgacggca gcgtgctcga
    46801 tcaggggatt gcgctatggt tccctggccc gaactcgttc accggcgaag atgtgctgga
    46861 actgcaaggt catggcggtc cggtgatcct cgacctgctg ttaaaacgca ttctgaccat
    46921 tcccggcctg cggattgctc gccctggtga gttttccgaa cgcgcgtttc ttaacgataa
    46981 acttgactta gcccaggccg aggcgattgc cgatcttatc gacgccagtt cggaacaggc
    47041 ggcccgttcg gcacttaact cgctgcaagg cgcattctcc gcacgggtta atcatctggt
    47101 agaagccctc acccacttgc gcatttacgt cgaagcggca attgatttcc ctgatgaaga
    47161 gatcgatttc ctctccgacg gcaaaattga agcccagctt aatgacgtga tggccgatct
    47221 cgatgcagtg cgtgctgaag cacgtcaggg tagtttgttg cgcgaaggga tgaaagtagt
    47281 gattgccgga cgtcctaatg ccggtaaatc gagcctgtta aacgcgctgg cgggccgtga
    47341 agcggcaatc gtaaccgata tcgccggaac cacgcgtgac gtgctgcgtg agcatatcca
    47401 cattgacgga atgccgctgc atatcatcga cactgccgga ctacgtgagg ccagtgacga
    47461 agtggaacgt attggtatcg agcgcgcgtg gcaggaaatt gaacaggccg accgcgtgct
    47521 gtttatggtc gatggcacca cgacagacgc cgttgatccg gcagagatct ggccggaatt
    47581 tatcgcccgt ctgcccgcga aactgccgat caccgtggta cgcaataaag ccgatatcac
    47641 cggcgaaacg ctgggaatga gtgaagtgaa cggtcacgcg ttaattcgtc tctcggcaag
    47701 gactggtgaa ggcgtggacg tgctgcgtaa ccatctcaaa cagagcatgg gctttgacac
    47761 caatatggaa ggcggcttcc tggcgcgccg tcgccactta caggcgctgg aacaggcagc
    47821 ggagcatttg cagcaaggta aagcgcaact gttgggagcc tgggcaggtg aactgctggc
    47881 ggaagagttg cgcctggcac agcagaactt aagcgaaatc accggggaat ttacttcaga
    47941 cgacctgctg gggcggattt tctccagctt ctgtattggt aagtaaccgc gcttacgaag
    48001 ccgtattctg actgtcagat gcggcttcgc ttcattgtta gcactcctgt tatgcctcaa
    48061 ccctttttta aacattaaaa ttcttacgta atttataatc tttaaaaaaa gcatttaata
    48121 ctgctccccg aacgattgtg attcgattca catttaaaca atttcagaat agacaaaaa
//
LOCUS       DAJVPP010000017        47410 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVPP010000017 DAJVPP010000000
VERSION     DAJVPP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.017"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OQM34_000983"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OQM34_000983"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS1451526.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OQM34_000984"
     CDS             complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OQM34_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1451527.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAAEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OQM34_000985"
     CDS             complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OQM34_000985"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1451528.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OQM34_000986"
     CDS             complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OQM34_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1451529.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OQM34_000987"
     CDS             complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OQM34_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1451530.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OQM34_000988"
     CDS             complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OQM34_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1451531.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OQM34_000989"
     CDS             complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OQM34_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1451532.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OQM34_000990"
     CDS             complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OQM34_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1451533.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OQM34_000991"
     CDS             complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OQM34_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1451534.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OQM34_000992"
     CDS             complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OQM34_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1451535.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OQM34_000993"
     CDS             complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OQM34_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1451536.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OQM34_000994"
     CDS             complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OQM34_000994"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1451537.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10283..11086
                     /gene="suhB"
                     /locus_tag="OQM34_000995"
     CDS             10283..11086
                     /gene="suhB"
                     /locus_tag="OQM34_000995"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1451538.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11231..12085
                     /locus_tag="OQM34_000996"
     CDS             11231..12085
                     /locus_tag="OQM34_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1451539.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12276..13556
                     /gene="csiE"
                     /locus_tag="OQM34_000997"
     CDS             12276..13556
                     /gene="csiE"
                     /locus_tag="OQM34_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1451540.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13548..14687)
                     /locus_tag="OQM34_000998"
     CDS             complement(13548..14687)
                     /locus_tag="OQM34_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1451541.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OQM34_000999"
     CDS             complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OQM34_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1451542.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15873..17234
                     /locus_tag="OQM34_001000"
     CDS             15873..17234
                     /locus_tag="OQM34_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1451543.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17231..17749
                     /locus_tag="OQM34_001001"
     CDS             17231..17749
                     /locus_tag="OQM34_001001"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1451544.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17749..18069
                     /locus_tag="OQM34_001002"
     CDS             17749..18069
                     /locus_tag="OQM34_001002"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1451545.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18066..18878
                     /gene="hcaB"
                     /locus_tag="OQM34_001003"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18066..18878
                     /gene="hcaB"
                     /locus_tag="OQM34_001003"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1451546.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18888..20100
                     /locus_tag="OQM34_001004"
                     /pseudo
     CDS             18888..20100
                     /locus_tag="OQM34_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20197..20619
                     /locus_tag="OQM34_001005"
     CDS             20197..20619
                     /locus_tag="OQM34_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1451547.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20667..21539)
                     /locus_tag="OQM34_001006"
     CDS             complement(20667..21539)
                     /locus_tag="OQM34_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1451548.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21551..22645)
                     /locus_tag="OQM34_001007"
     CDS             complement(21551..22645)
                     /locus_tag="OQM34_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1451549.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22678..23676)
                     /locus_tag="OQM34_001008"
     CDS             complement(22678..23676)
                     /locus_tag="OQM34_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1451550.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23701..25212)
                     /locus_tag="OQM34_001009"
     CDS             complement(23701..25212)
                     /locus_tag="OQM34_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1451551.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25235..26218)
                     /locus_tag="OQM34_001010"
     CDS             complement(25235..26218)
                     /locus_tag="OQM34_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1451552.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26315..29416)
                     /locus_tag="OQM34_001011"
     CDS             complement(26315..29416)
                     /locus_tag="OQM34_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCS1451553.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29423..29596)
                     /locus_tag="OQM34_001012"
     CDS             complement(29423..29596)
                     /locus_tag="OQM34_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451554.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29714..30907
                     /locus_tag="OQM34_001013"
     CDS             29714..30907
                     /locus_tag="OQM34_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCS1451555.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31105..32358)
                     /gene="glyA"
                     /locus_tag="OQM34_001014"
     CDS             complement(31105..32358)
                     /gene="glyA"
                     /locus_tag="OQM34_001014"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1451556.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32685..33875
                     /gene="hmpA"
                     /locus_tag="OQM34_001015"
     CDS             32685..33875
                     /gene="hmpA"
                     /locus_tag="OQM34_001015"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1451557.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGLVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33920..34258)
                     /gene="glnB"
                     /locus_tag="OQM34_001016"
     CDS             complement(33920..34258)
                     /gene="glnB"
                     /locus_tag="OQM34_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1451558.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34319..35653)
                     /gene="glrR"
                     /locus_tag="OQM34_001017"
     CDS             complement(34319..35653)
                     /gene="glrR"
                     /locus_tag="OQM34_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1451559.1"
                     /translation="MSHKPAHLLLVDDDLGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35643..36356)
                     /gene="qseG"
                     /locus_tag="OQM34_001018"
     CDS             complement(35643..36356)
                     /gene="qseG"
                     /locus_tag="OQM34_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1451560.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36521..37948)
                     /gene="qseE"
                     /locus_tag="OQM34_001019"
     CDS             complement(36521..37948)
                     /gene="qseE"
                     /locus_tag="OQM34_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1451561.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWLAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38524..42411)
                     /gene="purL"
                     /locus_tag="OQM34_001020"
                     /gene_synonym="purI"
     CDS             complement(38524..42411)
                     /gene="purL"
                     /locus_tag="OQM34_001020"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1451562.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42670..44226
                     /gene="mltF"
                     /locus_tag="OQM34_001021"
     CDS             42670..44226
                     /gene="mltF"
                     /locus_tag="OQM34_001021"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1451563.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44223..44759)
                     /gene="tadA"
                     /locus_tag="OQM34_001022"
     CDS             complement(44223..44759)
                     /gene="tadA"
                     /locus_tag="OQM34_001022"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1451564.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44784..45419)
                     /gene="yfhb"
                     /locus_tag="OQM34_001023"
     CDS             complement(44784..45419)
                     /gene="yfhb"
                     /locus_tag="OQM34_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1451565.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45628..46476
                     /locus_tag="OQM34_001024"
     CDS             45628..46476
                     /locus_tag="OQM34_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1451566.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46815..47369)
                     /gene="pinE"
                     /locus_tag="OQM34_001025"
                     /pseudo
     CDS             complement(46815..47369)
                     /gene="pinE"
                     /locus_tag="OQM34_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT        11308 a        12984 c        11919 g        11199 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa
      601 caatactcac cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag
      661 cgtattatcg acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag
      721 tccgaattca gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa
      781 gatattactt aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag
      841 gcggttattt gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa
      901 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
      961 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
     1021 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
     1081 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
     1141 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
     1201 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
     1261 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
     1321 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
     1381 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
     1441 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
     1501 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
     1561 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
     1621 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
     1681 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
     1741 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
     1801 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcag ccgcctgttg aagcgcttct
     1861 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
     1921 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
     1981 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
     2041 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
     2101 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
     2161 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
     2221 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
     2281 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
     2341 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
     2401 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
     2461 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca
     2521 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
     2581 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
     2641 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
     2701 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
     2761 cagcagagga acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc
     2821 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
     2881 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc
     2941 ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
     3001 gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
     3061 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
     3121 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
     3181 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
     3241 actttacgcg tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca
     3301 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc
     3361 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
     3421 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
     3481 atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc
     3541 cccaggcagc ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag
     3601 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
     3661 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
     3721 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
     3781 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
     3841 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
     3901 tcctgctctg agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg
     3961 gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg
     4021 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga
     4081 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
     4141 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
     4201 tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc
     4261 ggcagcattg tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag
     4321 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg
     4381 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
     4441 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
     4501 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
     4561 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
     4621 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
     4681 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
     4741 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
     4801 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
     4861 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
     4921 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
     4981 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
     5041 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
     5101 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag
     5161 ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc
     5221 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
     5281 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
     5341 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt
     5401 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
     5461 ctgacgctga gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
     5521 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
     5581 cgggttcagt agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
     5641 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
     5701 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
     5761 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
     5821 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
     5881 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
     5941 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
     6001 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg
     6061 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
     6121 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
     6181 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
     6241 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
     6301 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
     6361 gttccgccag gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
     6421 aacgcaggct cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
     6481 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
     6541 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
     6601 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
     6661 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
     6721 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
     6781 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
     6841 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
     6901 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
     6961 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
     7021 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
     7081 tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa
     7141 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
     7201 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac
     7261 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
     7321 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
     7381 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
     7441 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
     7501 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
     7561 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
     7621 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
     7681 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
     7741 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
     7801 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
     7861 cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac
     7921 ctttcggacc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
     7981 ggtcgattgg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
     8041 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
     8101 gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga
     8161 taataccgtt acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac
     8221 ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
     8281 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
     8341 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
     8401 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
     8461 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
     8521 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
     8581 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
     8641 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
     8701 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
     8761 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
     8821 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
     8881 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
     8941 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
     9001 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
     9061 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
     9121 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
     9181 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
     9241 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
     9301 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
     9361 cctacgattc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca
     9421 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
     9481 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     9541 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     9601 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     9661 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     9721 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9781 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9841 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
     9901 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
     9961 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag
    10021 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    10081 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    10141 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    10201 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    10261 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    10321 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    10381 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    10441 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    10501 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    10561 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    10621 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc
    10681 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    10741 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10801 caagctgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10861 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    10921 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10981 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat
    11041 actggcgaac atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt
    11101 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    11161 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    11221 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    11281 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    11341 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    11401 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    11461 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    11521 ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    11581 taaatcaaaa ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    11641 tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat
    11701 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    11761 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11821 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11881 cccgctttta ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa
    11941 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    12001 tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct
    12061 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    12121 caccttaaga catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac
    12181 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    12241 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    12301 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    12361 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    12421 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    12481 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    12541 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    12601 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    12661 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    12721 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12781 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12841 aggaaattgg acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac
    12901 tgtttatggc attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    12961 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    13021 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc
    13081 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    13141 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    13201 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    13261 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    13321 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    13381 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    13441 ttattgcgcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    13501 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    13561 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    13621 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    13681 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca
    13741 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13801 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13861 caaatcgccg agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc
    13921 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13981 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    14041 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    14101 gcagaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    14161 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    14221 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    14281 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    14341 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    14401 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc
    14461 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    14521 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    14581 aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag
    14641 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    14701 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    14761 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    14821 acgcatgttg caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14881 atggcgatga aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa
    14941 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    15001 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    15061 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    15121 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    15181 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    15241 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    15301 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    15361 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    15421 ggcaataaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    15481 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    15541 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    15601 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    15661 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    15721 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15781 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15841 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15901 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15961 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    16021 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    16081 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    16141 tgcgtgtgag ttatgcagat tgtggcaaca ctcgcgcctt tacctgcccg tatcacggct
    16201 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    16261 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    16321 gactgatttt tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca
    16381 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    16441 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    16501 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    16561 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    16621 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    16681 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    16741 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    16801 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    16861 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16921 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16981 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    17041 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    17101 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    17161 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    17221 aggtgatgaa atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct
    17281 ctttcacgaa gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga
    17341 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa
    17401 aggcgtccag ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg
    17461 aatcgcccgt ctggaaacgg gcattgcctg ggcagaagag ccgccgtcac gcactcgtca
    17521 cttaatcagc aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt
    17581 gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    17641 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    17701 cctggatcaa gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    17761 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc
    17821 gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17881 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17941 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    18001 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    18061 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    18121 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt
    18181 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    18241 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    18301 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    18361 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    18421 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    18481 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca
    18541 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    18601 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    18661 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    18721 cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc
    18781 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18841 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    18901 cgatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18961 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    19021 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    19081 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    19141 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    19201 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    19261 gctttaccct acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    19321 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    19381 agcgcagcgc agcgcagatg taaggtgaca gtgattgaac tggcggcaac cgtcatgggc
    19441 cgtaatgcac caccgcccgt gcaacgctat cttttacagc gccatcagca ggctggtgtg
    19501 cgcattctgc tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt agaactgacg
    19561 ctgcaaagtg gggaaacgct tcaggctgat gtggtgattt acggtattgg tatcagcgcc
    19621 aacgagcaac tggctcgcga ggccaacctt gatactgcca atggcattgt cattgatgag
    19681 gcttgccgca cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat cactcgtctt
    19741 gataatggtg cactacaccg ctgcgaaagc tgggaaaacg ccaataacca ggcgcaaatt
    19801 gccgctgccg caatgttagg gctaccgcta ccgctactgc cgccgccgtg gttctggagc
    19861 gatcagtaca gtgataactt acagtttatt ggcgatatgc gtggcgatga ctggctttgt
    19921 cgtggcaacc cggaaactca gaaggcgatt tggtttaatc tgcaaaacgg cgtgcttatc
    19981 ggtgcggtca cgctgaatca gggccgtgag attcgcccaa ttcgcaaatg gatccagagc
    20041 ggcaaaacgt ttgatgcgaa actgctgata gatgagaaca tcgcgcttaa atcactgtaa
    20101 tcaggataat tagcgaatat ctcaatgcct ggggcgtggc gaggtgcaag agtgtgtatt
    20161 acgtttaaat cacattatct tgcaaaggga ttggttatga acacactacg ttattttgat
    20221 tttggagctg cccgccccgt tttgttatta attgcccgta tcgccgtggt cttaattttc
    20281 attatttttg gttttcccaa aatgatgggc tttgacggta cggtccaata tatggcctcg
    20341 ttgggcgcgc caatgccgat gctggcagcg attattgcgg tagttatgga agtgcccgcc
    20401 gcgatattaa tcgtgcttgg ctttttcacc cgtccgctgg cggtgctgtt tattttctac
    20461 acgctgggta cggcggtgat tggtcaccat tactgggata tgaccggcga tgcggttggg
    20521 ccaaatatga ttaatttctg gaagaatgtc agtatcgctg gcgcgttctt gctattggca
    20581 attaccggac cgggggcaat ttctctcgat cggcgttagg aaagatgccg gatgcggcgt
    20641 gaacgcctta tccggcattt tataaattac agccactcta cccgcaacga catctctgag
    20701 gttgttgatt cccctggtgc tagcgcaatg agatcacccc cttccgggcg atgatgatca
    20761 tccggcgcat ggctcatcgg ttccagacag aagaaatcaa acgcatatcc tttatcaaac
    20821 gcagggtcgg aaacaaagat gaaataacac ggtgcaggtg gcgtcgtttc catgatgatg
    20881 gcataacctt cctgcggctg ctcgatgcgg gcctgaccat tccatccggc aaaaccattg
    20941 ttcacccact gacgcggcaa cggcgcgagc tggttaaaat ccagttcctg cggtagctgc
    21001 tcgcaaaact cacccgccag ccactgctcc cgttccagcc agtaaccgct cgcctgcgcc
    21061 tgaatccgcg tttgtggtga caacggaaaa taaggatgcc agccggtacc aaatggcagc
    21121 gtctctgccc cttgattggt gacagagagc gtcaccgtca gcgtatccgc cgttaaatga
    21181 aacgcctgac ttacccgata gtgatagaca ccgctgcgat gttcatacac cagacacaaa
    21241 ctatcatcgc tgtgcgagac acattgccac tcgcccagcc agccatcgcc gtgtagatag
    21301 tgcgcatccc actcaacatt cggttgcagt tgatactcac gcccctgcca gacaaaacgg
    21361 ttgccgctca cccggtttgc aaatggcacc agcggaaaac atgaggcatc ggttgccaca
    21421 ccgctttttt taccaggacg taagagcggc gtcgtatcgc gccagaagcc ttcgattacg
    21481 ccgccctggt cagaaacgtc cagctttagc gacccgtggg ataaggtata gatggtcatc
    21541 cgctaactcc ttaatccggg aagttaatca caactttccc gcatttgccg ctcgccatca
    21601 gcacataggc atcacctgcc tgttctagcg agaagcgatg ggtaatggcg ttacgcggcc
    21661 acagcttcca gtcagtcaaa tcgtgggcgc atttttccat atggaacaga ctggtcaccc
    21721 aggagccgat aatccgccgt tgatggtgca tcagatcagc gctgacctcg aattccactt
    21781 ttccggtttc accaatgtaa accacccgcc cccagtcagc agtggattgc agtgccagca
    21841 agcgacctgc ggcattaccg gaacaatcga gcgcaacatc cgcgccaccg tgggtaagtt
    21901 cagcaattat ctgcggcaga ccttcggtgg ttgctaaata gccgtgatcc atccccccta
    21961 actgttttgc catcgccaga cgttccggca gcatatcaac gccgatgatc cgttttgcac
    22021 cgcgaccttt cgccagcatc atcgccatca tgccgaccgg ccccagaccg accaccagca
    22081 cgttatcgct gccggaaact tcgccgcgca aaatcccttc ataggccgtg ccaacgccgc
    22141 aactgataaa cgcaccatct tcgtagctca gcgcatccgg caggaggatc agatcttttt
    22201 cttccgccag cagatattcg gcatgaccgc cgtcacgctg ccagccgtaa gccgcttttc
    22261 ctttgccagt acaagagata ggaaagccgc ggcggcagtt cgggcaaaaa ccacagccag
    22321 aaatgtgata caccagcacg cggtcgccct ctttaaaatg gcggcagcct tgccccatcg
    22381 ccacaatctg cccgcacggt tcatgaccgt tgataaatcc ctggtataac ggtttatcgg
    22441 gtgccgccgc cgtggcacgg tgttgatgat agatatagtg gacatcgctt ccgcaaatcc
    22501 cggaggattt cattttgatc agtacctggt taatccccgg cgtcggcacc gcaacttccc
    22561 gcagatcgac ggttgaattt cctagtaaat aagcagccag catcgttttc ataaatccct
    22621 cattaacaat acgattaatt ttcatccctg ccgcacccgc gccaggggcg ttaaggttta
    22681 gcgtttcgct ttactgcttc gctgggtcag caagatattc gccagcaccg ccaccacgat
    22741 gatgacgccg cgtaccacct gctggaaaaa ggagttaata ccgagcagca ccagaccgtt
    22801 accgattagc gtaatcacca gcacaccaag caatgtaccg aacagggaac cgcgaccgcc
    22861 ggaaagtgcc gtaccgccga ccacgaccgc ggcgatgacg tcaaactcca gaccgtttgc
    22921 ggcacctgcg ttaccggaac cgaggcgtgc cgccaacaaa atgccggtca ccgccgctaa
    22981 taatcccgaa agggtaaaga taagaatgcg cactcgacga acgttaatac cgcacaactg
    23041 cgccgccgtg gcattaccgc caacggcaaa aaccgagcgc ccgaaggcgg tttttcggct
    23101 aatgaacaca aacagcgcaa acaacaccat catgatcagc gcggatactg gcacaccgag
    23161 aaattgtccg cccagccagt ccagcacctc gttttcgtca atcggcactg gcagtgcgtt
    23221 ggtcataaac agccccattc cgcgcagagc gctccacagc cccagtgtgg caacgaaact
    23281 tggtacgtta aacacgccgc gcagcacccc ggcgagcgtt cccatcagcg cgcctaacag
    23341 caacaccagc agacaagcaa tcgccaacgg aacttcaaat tgcagcaaaa atgccaggca
    23401 caccgagaca aaagccacca tcggcccaac gctgacatca atttcaccgg agataataat
    23461 cagcgtcatc gcccaggcgg caatcccaat ggtggcggca tcgcgcagca cgttcatctg
    23521 gttattcaat gagataaagc caggcgcgtt cagggagaag accagataaa gaatggcaat
    23581 caccaccagc aaaccgatct cattaatatg gcgactgaca aattgtttga gcgagacgct
    23641 cttgccctgc ggcaatggta atgacgaagc atacatcttc aggttcctcg gtaaaattca
    23701 tcagtgcaca gacagaatgg cggacatcag ctcatccaca ttgaccggcg agtgaaactc
    23761 ctgcgagaac gtgccgtgct gtaataacag gatgcggtca cacaccagcg gcaattcctc
    23821 cacttcactg gagataaaca ccacgctttt tccttcggca gccagctcac ggacaatacg
    23881 gtaaatctgc tgtttggctt cgatatcgac gccgcgcgtt ggctcgtcga gcaacaaaat
    23941 ctggctggcg gcatagaccc aacgaccgat caccactttt tgctgattgc caccagaaag
    24001 cgtgccgatg ggtgtttcgc tactggcagc cttgaccgtc atccgctgca tcacctcttc
    24061 ggtcaggcgg cggatggtgg accattgcag cacaccgttg gtgctgattt tttgccgatt
    24121 ggtcagcact gtattttcgt caacgcctag ccagggaatg atccccgctt ctttgcggtt
    24181 ttctggcgta tagccaatgc cgcgtttcag catgtcgccg taatcggggc gcatgatttt
    24241 ctcgccgttg ataacaattt cgccctgttc atacgtctcc agcccaacaa tcgccttcag
    24301 caattcactg cgccctgccc ccagcaggcc agcaatgcca agcacttcgc cacgacgtag
    24361 cgtaaagctg atatcctcca gcttgggctt atggcgtaac gcacggactt ccagtacggc
    24421 ctgatccaca atttcctgag gggcaaccgg cgcaatatca acgtgatcac gccctagcat
    24481 tagcgacaca atatgatgcg tggaggtgtt ttcgagcatc acatcgcccg ccacctgacc
    24541 atcgcgcata acggtggcat aggaggcaat gcggcggatt tcttccatcc ggtggctgac
    24601 ataaatcacc gccacgccca gtgccgacat ctttttcacc gcactgatca ccagttcaac
    24661 ttctgcactg gcaagcgagc tggtgggttc atcaagaatg accacgcgcg gctcgccctt
    24721 catcacccgt gcaatttcca ccagttgctt ttgcgccggg cttagcgttg aaacaagttg
    24781 ttcaggacta acgtcaacgc ccagcgcctg taagcaacgt tgggcatcct gcgccatttg
    24841 caggtaatca atcatgccgt tgcggcgagg ccattgacca aggcagaggt tttctgccac
    24901 cgtcagccct tccaccagac ttaattcctg ataaaccgca cgaaccccca gttcagcggc
    24961 acggcgagtc aacgtagctt cgtcaccttc cagtcgcgtc tcgccaatcc agatatcacc
    25021 gctatccggg cgttcgctgc cggtaagcat tcgaatgaga gtcgatttgc ccgcgccgtt
    25081 tttacctaac agcgcacgaa cttcgccttt attgagcgtg aagttaacgt tatccagcgc
    25141 aacgacgccg gggtaacgct tatttcctgc caccactttt gctaccggga ctgcctctgt
    25201 tgccgtgaac atagggacct ctgcgaatca gcgattaggg cagaccatca acgtgcgttg
    25261 ccagccactg tttaccatct tccgttttgg tatagagatc gattggcacc tgaatcactt
    25321 tttcaccgtc ggcttgttta ttgataacct tcaatgtttg cgcgaaaaca gcattgccca
    25381 ttttcttacc ggaaatatcc actaccgctt tcagcacctg attgttttcc agctcctgag
    25441 caatttcggt tgtcatatcc gaaccgaaaa cagcaatttt tccggcctga ttttgattac
    25501 gtaccgcttt taccgcgccg agtgtcgcac cgcccgactc ccccataatg gcgttgagat
    25561 ccggcgtgga gataatcagt ttttcaccaa cggaaatcgc tttatctaaa acagtccctt
    25621 cctggttagc gacaatttgc gcgccgggaa cgcgggattt taatacttct tcaaatcctt
    25681 tacgtcgctg cacacaaact tcaaaggctt cgcaattgat gacggcaatt ttcggctggt
    25741 caattttatt ggcaataaaa taatcggcgg cagcgttacc cagtttttta ccaaattcca
    25801 gtggatcgcc gaccagatac gccgagacat atttatcgac acccttttga ttaatacagg
    25861 tgttgtagca aatcaccgga atacccgctt cactggcgcg acgaacggta cggctactgc
    25921 cattttcaga cactgccgat aaaataatgg catcgacatt acgcgccacg agggtatcaa
    25981 caaaggtact ttctttcgaa atatccccct gggcgttagt ttcaattaac tgtacctgta
    26041 ctgaagaatc ttttgccgca tcctgaacgc cctgacgcac tccagcgtaa tatccctggg
    26101 tatcaaggta tattgcacca atggtcattt ctttatccgc agccctggca aatagtgcgc
    26161 tacctaatag cgtagccatt aataatagat tacgggttgt tctcattttt ttaggcatag
    26221 tgcctcctgt agggttttta ttaacaacgg cttattctaa ttattttgtg atgagcggca
    26281 gcgcgtgccg ctcaaatatt acagttaata gggtttagtg aacgtagtta aaaataaaag
    26341 gcatcacagt ggtgaataat gccgctttcg gccaggccgg attaatcgcc gtcaattcag
    26401 cgcgagcaga ctcatattgc gcaacctccc cgagtcccgc ggacgccagc atcgccacca
    26461 tcaggcattt ttctttatgc tgctgttgta aatcgccata cagcgataac aggtcaggtt
    26521 gtgaaacagc aaagaaatcc gcctcaatac tggttttcgc catctcttgc gcccactgtt
    26581 tcatttcact aaacagttgc tgtgcggttt gctgttcacc cagcagtcgc agcgccattc
    26641 cttgccagaa gagataatca accggctgat cgttgtaata actgtggatg ttaatggtgc
    26701 gatcgccggt cgccgccaga cgtaaacaac gcatcgcttc agtttcatcg ccctgcgcgt
    26761 tggcgcatat cgcctgccag aaccagatgt cgttatcagt ttgccccggt aaacggcctt
    26821 cgcttaaatt ctccggataa tacagcgcgg catgaagcag ttcgctggcc tgctgcggct
    26881 ctctggcatc aagatgctgc caggcgcgta ataactggtt gaggataaac tgactggtga
    26941 ccttcccttc cccgccttcc cacgggtgga atttacgcgt ggcgagaatg tccgccgctt
    27001 tgtctgcctg acccgtgaga tgccacaaat tgagcagttc tgcggtcatg tcgtcgcgtt
    27061 tcagcgcaat ttccagatta ttttccagac gtgccagtcg tttctccggt gtggctccac
    27121 ttaacttatc cagcaaatcc cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat
    27181 aagcattatc aagataacgc gcggccagct cataatcgtg tttcttattc cacgcatgga
    27241 tcgctaaccc gcgccagccg tcggcaaact ccggcgacat ctctacgcaa cgttgccata
    27301 aggcaatggc tttgttgtag ctacgtttgt tgtagtagaa gcaggccagt aaatggcgag
    27361 caaaccagca ctcttcaata ctctccagcg ccgccacttc ttccagcgta ttcgggaaac
    27421 ggacaaactg cgggaagaca tcaatggctt ttgcgaccag ttcgccacgt tcggctttcg
    27481 gcagcaagct ggcttgcagg taaagcggca gcgtgcgctg gcagtccagt gcgttcagca
    27541 tctctgccgc cagggtgggc attccccagt taatcagttg cccggcggtc atcagagcgt
    27601 taacgtcgcg tccctgacac agaccgcgcc actgcgcgag ggctgattcg ctacgaccat
    27661 cgaaccagtt cagccaccac agagtggcgt tcagcggata atcacgcagc agtttctcgc
    27721 gctgcaaacg cgcgttgtcc tgacgaccac ttaacaccag cagcagatta tgcaagcaaa
    27781 gcacttcctg attggttggg caggcacgaa gactttgttg gcaaaaatcc agacccgcgt
    27841 cgaagttatc attacgcgcc gccagtcgtg ccagaccata atagccgcct gctttactat
    27901 tgccgctcca gaccgcacgc cagaaatcct cttcggcttg ttgatattgt ccctgacgtt
    27961 cgtaagcact ggcgcgaatc aaacttgctt gtccacactg cgggtttttg ttcagcgcat
    28021 gtgcgcgttt cagcgcctga ctggcatacg ccaccgcttg cgggaaatct gcgcggttat
    28081 attccagcat cgccagcgcc aggttacagc gataatccag cgggtccagc gccacgccgc
    28141 gcaggtagta atcgaacggt gaacggctgg cgtgatgata ttgctccaga tgctgaccga
    28201 taaaccaggc ttcatctgta ctggtaatgt cttgtgctgc cagtggcgct ttggcgacgt
    28261 ccggcaacgg caactcctgc ggctgatgtt cctgataact cagtacaata ttgccgtcgg
    28321 catcggagag ttcaatggtc agtctgtcag gattgatacc gtgcaacacg ccctggatgg
    28381 cggtcgctgg tgtcagggcc acggcatcat cgagcaacgc gttgcatttg ccgatttcgc
    28441 ggatcgccag acggtatccg ttcaacggag agatggcata cagcccccac tcaatccccc
    28501 gctcactacg ctggagtttt atcaccgcat cgcgggaggc attttgcacc atgcccagag
    28561 aatgataagg caggaaatac tgctcgaaac gcttctcttc gtaagcatca agccaggtaa
    28621 aatcaggctg gttatcggca aaaataccgg tcatcagttc gatatacggg ccgttattgt
    28681 cggtcagact cttatcccac gcctggccaa attcactgtg tccccaactc cactgttttt
    28741 tacctggcgc aatatggtgg ttggcaacgt gcagcaaacc gccatcttca tcgtgacacc
    28801 acgcgccgac aaaatcgtac tgcgattttt cggccatata tgaggttgga acaggcacat
    28861 ttttatagcg agaaatgtcc actccggcgg agtagtccac tttgtagtaa gtgccggtgg
    28921 cgatggggaa agcggagacg gcccgtttgc cgtgatcaaa caccgcagtc acatccggtg
    28981 ggaaaacgct ctgatgccct tcaccccctt tcactgccgg gttggcccac cacaagaaat
    29041 gacgcggcgt ggcgttgccg ttatagacgc ggctggcgat ttccagtgcc gcccggtcag
    29101 ggcgcagggt gaaacctgtc atcacctgta aaccatgcat cggctccgtt tcgccgaccc
    29161 acaccgtctg tgcaccgtct tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg
    29221 tcgggcgatg gtgttgcggc cagttaaact caatcccgcc ggagatccac ggtcccagca
    29281 tccccaccag cgcaggttta atgacttcat tgtgatagac aaaatcgcgc tgtttcactt
    29341 tatcccatgc gcgatgcacc cgaccgccca gttccggcag gatcatcact ttgatgtagt
    29401 cgttttccag ccacaccgcc tgctaggatt tcagggtttt ctgctcgctc agcgtatcgg
    29461 tcacgccgta gggataaacc gcgccggacg atccctgata aacgcgattt tccaggaaca
    29521 tgggatgtat atcctgcggc ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca
    29581 cttttactgg agtcatggtg ccctcaatat taacaagaca tactgaatta aaagatttgt
    29641 ggcagtgtat tgaacaatct ggcaatgttt tcgcggaata atcacgcaat taactaaaca
    29701 aggtttagtg aagatgagag cctgcattaa caatcaacag attcgccacc ataacaaatg
    29761 cgtgattctg gaactgctgt accggcaaaa gcgcgccaat aaatcaacgc tggcccggct
    29821 ggcgcaaatt tcgattccgg cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg
    29881 ggtggtgaat attgacgatg aaagccagac gcgcgggcat agtagcggta catggctgat
    29941 tgcgccggaa ggtgactgga cgctgtgcct gaacgtgacg cccaccagta ttgagtgtca
    30001 ggttgctaat gcttgtttaa gtccgaaagg cgaatttgag tatttacaga ttgatgtacc
    30061 gacgccgcag gcgctgctgt ccgaaatcga aaaatgctgg catcgccacc gtaaattgtg
    30121 gccggaccgc accatcaatc tggcgctggc aatccacggg caggttgatc cggtgaccgg
    30181 cgtatcgcaa accatgccgc aagcgccgtg gacaacgcca gttgaggtga agtatctgct
    30241 ggaagagaag ctgggcattc gggtgctggt cgataatgac tgcgtgatgc tggcgctggc
    30301 ggagaaatgg caaaataatt cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg
    30361 cattggctcg tcgttcgtga ttaacgagca aatttatcgc ggcagtttgt atggtagcgg
    30421 acagattggt cacaccatcg ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg
    30481 cagcctggaa accgtcgcct cgttaagtgc attaaaaaaa caggcgcggg tatggctaaa
    30541 atcacaaccg gttaatactc agcttgagcc tgaaaaactg acaaccgcgc agttaatcgc
    30601 tgcctggcag agtggagaac cgtggatcac cagctgggtt gatcgctctg ccaatgccat
    30661 tggtttgagt ctgtataact tcctcaacat cctcaatatt aatcagattt ggttgtacgg
    30721 tcgcagttgt gcctttggtg agaactggct taatactatt attcgccaga caggatttaa
    30781 cccgttcgac cgcgacgaag gaccgagcgt gaaagcgacg caaattggct tcgggcaatt
    30841 aagccgcgca caacaggtgc tgggaattgg ctatttgtat gttgaggcgc agttacgaca
    30901 gatttgatgg cgcgataacg tagaaaggct tcccgaagga agccttgatg atcataaacg
    30961 aaaaattgcc tgatgcgcta cgcttatcag gcctacacgg agattgcaat atattgaatt
    31021 tgcaagattt tgtaggcctg agaaggtgtt catgccgcat ccaacatgaa caacgagcac
    31081 attgtcagca aatcaccgtt tcgcttatgc gtaaaccggg taacgtgcgc agatgtcgag
    31141 aactttacct ttgatgcgct cgataacggc ttcatcattg atgctgtcca gcacgtcaca
    31201 catccagcca gccagttctt tcgcttcggc ttctttaaag ccgcgacggg taatcgccgg
    31261 agtacctaca cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg gtacgctgtt
    31321 tttgttgacg gtgatgttag cacggcccag agcggcgtct gcttctttac cggtcaggtt
    31381 tttatcaacc agatcaacca ggaacaggtg gttatcagtg ccgccggaaa ccactttgta
    31441 gccgcgctcg aggaacactt ctaccatcgc tttagcgttt ttcgcgacct gctgctggta
    31501 agttttgaac tcaggctcca tcgcttcttt cagagcaacc gctttaccgg cgattacgtg
    31561 catcaacgga ccgccctgac caccagggaa aacggcagag ttcagttttt tgtacagctc
    31621 ttcgctacca cctttcgcca agatcaggcc gccgcgcgga cccgccaggg ttttgtgagt
    31681 ggtggtagta acaacgtgag catgaggaac cgggttcggg tagacgccag cagcaaccag
    31741 gcccgcaacg tgcgccatat caacgaacag ataagcaccg atgctgtcag cgatttcacg
    31801 cattttcgcc cagtcaacca cgccggaata tgcagagaaa ccaccgataa tcattttcgg
    31861 cttgtgttct ttggcttgtt tttccagatc ggcgtaatcg atatgaccgg tagcatcgat
    31921 accgtaagga acgatgttgt acagtttacc ggagaagtta accggagaac cgtgagtcag
    31981 gtgaccgcca tgcgccaggt tcatacccag aacggtatca cctggttcca gcagcgcggt
    32041 gtagaccgca aagttagcct gggagccgga gtgcggctgg acgttagcgt agtcagcgcc
    32101 gaacagttct ttcgcacgat cgatcgccag ttgttcaacg atatcaacat actcgcaacc
    32161 gccgtagtag cgtttgcccg gataaccttc agcatatttg ttggtcagct gagaaccctg
    32221 cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga tgtgctcttc
    32281 ctgacgtact ttttcctgct ccatagcctg ccacagttcg gcatcataat cggcaatgtt
    32341 catttcacgc tttaacatcc gcatctcctg actcagctaa caataaaatt tttggccttt
    32401 ataggcggtc ctgttggaca acggcgaaca gtataaccga atcattgtgc gataacaggt
    32461 cttgacaaag gaatttacgc aaacgattac cttcaggcta cgcaaggctt tggagaataa
    32521 agagcttgca accggaaacg gatttctttt caggtttgtg atgcaaattt ttcacttcat
    32581 cacattcttt ctgaaaaaca ccaaagaacc atttacattg cagggctatt ttttataaga
    32641 tgcatttgag atacatcaat taagatgcaa aaaaggaaga ccatatgctt gacgctcaaa
    32701 ccatcgctac agtaaaagcc accatccctt tactggtgga aacggggcca aagttaaccg
    32761 cccatttcta cgaccgtatg tttactcata acccagaact caaagaaatt tttaacatga
    32821 gtaaccagcg taatggcgat caacgtgaag ccctgtttaa cgctattgcc gcctacgcca
    32881 gtaatattga aaacctgcct gcgctgctgc cagcggtaga aaaaatcgcg cagaagcaca
    32941 ccagcttcca gatcaaaccg gaacagtaca acatcgtcgg tgaacacctg ttggcaacgc
    33001 tggacgaaat gttcagcccg gggcaggaag tgctggacgc gtggggtaaa gcctatggtg
    33061 tactggctaa tgtatttatc aatcgcgagg cggaaatcta taacgaaaac gccagcaaag
    33121 ccggtggttg ggaaggtact cgcgatttcc gcattgtggc taaaacaccg cgcagcgcgc
    33181 ttatcaccag cttcgaactg gagccggtcg acggtggcgc agtggcagaa taccgtccgg
    33241 ggcaatatct cggcgtctgg ctgaagccgg aaggtttccc gcatcaggaa attcgtcagt
    33301 actctttgac tcgcaaaccg gatggcaaag gctatcgtat cgcggtgaaa cgcgaagagg
    33361 gtgggctggt atccaactgg ttgcacaatc acgccaatgt tggcgatgtc gtgaaactgg
    33421 tcgctccggc aggtgatttc tttatggctg tcgcagatga cacaccagtg acgttaatct
    33481 ctaccggtgt tggtcaaacg ccaatgctgg caatgctcga cacgctggca aaagcaggcc
    33541 acacagcaca agtgaactgg ttccatgcgg cagaaaatgg cgaagttcac gcctttgctg
    33601 atgaagttaa ggaactgggg cagtcactgc cgcgctttac cgcgcacacc tggtatcgtc
    33661 agccgagcga agccgatcgc gctaaaggtc agtttgatag cgaaggtctg atggatttga
    33721 gcaaactgga aggtgcgttc agcgatccga caatgcagtt ctatctctgc ggcccggttg
    33781 gcttcatgca gtttaccgcg aaacagttag tggatctggg cgtgaagcag gaaaacattc
    33841 attacgaatg ctttggcccg cataaggtgc tgtaatttga tgttgccgga tggaaacatc
    33901 cggcaaccct tgacgcggtt taaattgccg cgtcgtcctc ttcaccggta cggatgcgaa
    33961 tgacccgagc cacgtcaaag acgaagattt taccgtcacc gattttgccg gtttgcgccg
    34021 tgcgaataat ggtatcgaca caggtatcga caatgtcgtc cggtacgaca atctcaattt
    34081 tcactttcgg cagaaaatcc accatatact ccgcgccgcg gtacagctcg gtatggcctt
    34141 tctggcgacc aaagcctttc acttcggtca ccgtcatgcc ggtaataccg acttcggcca
    34201 gtgcttcgcg gacatcgtcc agcttgaagg gttttataat cgcatcaatc tttttcatgc
    34261 tattccttga aaaggttgcc tgtcttttga tctgctaaac gtaacacata acgccaattc
    34321 attccttgaa atcgtttgca tccagctcgt gtcgggaaag cagtttataa aattctgtcc
    34381 ggttgcgccc cgccattctc gccgcgtggg tgacgttgcc tttggtgatt tgcagcagct
    34441 tacgcaaata gttgagttca aaatgattac gtgcctcaac aaaggttggc agcgccgtat
    34501 tttcaccctc cagcgcctgc tccaccagcg catcactaat caccggagat gaggtcagcg
    34561 ccacgcactg ttcaatcacg ttgaccaact ggcgcacatt acccggccag ctcgcggtca
    34621 tcaggcgttt catcgcatcg gtagagaacg cgcggacaaa cggtttatgt cgctctgccg
    34681 cctggcgcaa caggtgattt gccagtagcg gaatgtcttc tgtgcgctcc gccagtgccg
    34741 gaattttcag gctgacaacg ttgaggcggt aatagagatc ttcgcggaat tccccgcgcg
    34801 ccatcgcttt tgacagatca cggtgagtgg cagaaataat ccgcacattg atatcaatat
    34861 cgcggttact gcccagcggg cgcactttac gctcctgcaa cacgcgcagc agtttgacct
    34921 gtaacggcgc aggcatatcg ccaatttcat cgagaaatag cgttccgcct tccgccgcct
    34981 ggaataaacc ttcgcgattg ctgacagcgc cagtaaacgc gccacgcgca tgaccaaaca
    35041 gctccgactc cagcaattgc tcgggcaatg cgccacagtt aatagcaata aatggtttgc
    35101 tgttgcgcgg gctggcgttg tggatagcct gggcgaaaat ctctttcccg gtgccgctct
    35161 gaccgttaat caaaacgctg acgtctgatt gcgccaccag ccgcgcctgt tccagcaaac
    35221 gcagcatcag cgggctgcgg gtgacaattg cctcgcgcca gcgttcatcg gtggctggcg
    35281 cggattgctc cagcgcatcg tcaattgcct gatatagcgc atctttatcg acaggcttgg
    35341 tgaggaaact aaaaacgccc tgctgtgttg cagcaacggc atcggggata gaaccatgcg
    35401 cagtaagaat aattactggc attcccggct gcactttctg gatttcagca aatagctgca
    35461 taccgtccat ttcatccatc cgcaggtcgc tgataactaa atctactttt tcgcgattca
    35521 gtacccgtaa tccttcagcg ccactttccg ccgtgaccac actgtagcct tcgctggtca
    35581 ggcgcaggcc aagcagtttc agcaatccca gatcgtcatc gaccaataat aaatgcgcag
    35641 gtttatggct catcaggagt gacctcatgg gtggatggcg cgggcttttc actctcgtgc
    35701 ggcgtatccg gcgagaaatt tccagccggt ttgcgggtcg agagctggcg ttcaatatcg
    35761 gtcaggtttt ccagcttgcg ggtggtgagt tccagttgct gctgtagaac gtgatgttgc
    35821 tggcgcaatg tatccagctc gctgtcgctg gactgctgga gtttactgta acgttggcgc
    35881 tcttccgcca gttgcagttg cagcgcctga ccgtcgcgcc agagttgata cagtgggcga
    35941 acctgtgccg ggatctcggt acttaacgct tcaatacgcg cgaccagctg gcggcgctca
    36001 tacggcgtaa ttttggcgtc ggcgagcaaa atcccttgtt taaaggtatt ttgccagctg
    36061 ccgtcgtcat attggcgagc ttgctgacgc gactgcgcag gcattaaacg atcagcacaa
    36121 tccatcgccc gcagccagta aagcggattg gtttcggttg atttaccttg cagcgcccag
    36181 atgtcgctac attcagtaga aagatagtca gccagttgat aaaccggaat tttttcttct
    36241 gctggcgtat caatggctgg cttattgtga ttctgcacgc aacccagcaa tgccagacat
    36301 ggcagccctg ccagccacag ccgtcggggc aataatcgtt gaaaaatgtg tcgcatattc
    36361 accagactta aagcctatcc cagtgggcgt aattgttgca gacagtctgg acatggacag
    36421 cgcggagaaa ccggggcgta catatcgtac gtgaagattt cgagcactac ccggggccga
    36481 actgacaaat aaaatagcct gataggatag gctgttagca ttatttcgtg tttttcgacg
    36541 acggtaattc aatgcggaaa caaacgtctt gcccgctctc gtcgaccaga tacagttccc
    36601 cttgcatacg gcgaatacaa tccctagcaa tgcttaatcc cagaccgctg cccttcaccg
    36661 ccccttttcg ctggtggctt ccctgaaaaa aggggtcgaa gatcatggcg cgttcctctt
    36721 gcggaatggg cgtgcctgta ttgatgacat caatataaac ccgcgcacca tgtaaactgc
    36781 tgcgaaggca aatgttaccg gattcagccc cgtagtgcac cgcattggag taaagattat
    36841 ccagtacgct catcagcagc attggctccg ccaggcaagc tgttgctttg agatcgacgt
    36901 cggtatgcat cattttagcc cgtgcgggca ggctatgagc agaaaccact gtctccacca
    36961 gcggtgctaa ttcaacattc tccagttcca ccgcactgtc cgcctgttta cggttgtaat
    37021 caagcagttg ttcgatcagt ttttgcaaat tgcagctgct gctatcaaga atgctcacca
    37081 cctctttttg ctctggcgta agcggcccga caacctgatc agccagtaat tcagtgccct
    37141 cgcgcatact cgccagtggc gtttttaatt catgagataa atgtcttaaa aattgatggc
    37201 gttgggattc cagccatgac aggcgctcac ttaaccaaag aatacgttgc ccaaccgagc
    37261 gtaactcgct cggtccacag aacgaaacgc tattgcccag agaacgccct tcccccagac
    37321 ggttgatcat gcgctcgata tttttcaccg gcccgataat catccgcgtg aaaagtagca
    37381 ccatcaccag actcaccaga aatagcacca gcgattgcca accaaaatat tgcccacgtt
    37441 cggcgatttc acgctgaagt tgctgcccac gagagaacac cactgtgcgc gtggcctgca
    37501 ccatttcggt attggcactg gcaaaggctt caagacgcgc ggcggcggca gcatcgggac
    37561 cactgttgtt acactgaagt tgggccagat tgttcaaatc ctgacgcaat gcctggtaaa
    37621 gtttatcgtc tggcagtacg cctgcgtggg catcgagcat ttcgctgtaa cgcttgcgct
    37681 ggctttgata aaccttcgca agcgtagggt cgtccagcac gcaatactga cggtagctac
    37741 gctccatctc cagcgccgcg ttggtcatcg cttcactacg ccgggcatca ataagcgtag
    37801 tgcggttaac cagcgccgcc tgatcgctaa gcgcattcag gctttgccag gctagccatg
    37861 ccagcaccaa caggggtagt agaattagca aaaatgccag cattaccagt tgtcgtaatg
    37921 agcggggaaa aacgggccag cgtttcaagg tgttactctc gtcagacgcg aatagcctga
    37981 tgctaaccga ggggaagttc agatacaaca aagccgggaa ttacccggct ttgttatgga
    38041 ataaggcggt gcctaactcg acgtttcgcc cgatggttga tatagctacg ctgatatcag
    38101 aagttggacg gcaggcacct tgttgtgcgt cattcgaatt ttatgtagca cgtcccgaag
    38161 gggctgacat aagtcggtga atgagccact gcttactatt atgcagtaac tgtgccaata
    38221 aagaaaatag tttggtaacg tactgattat acgttgcttt gagggattta tggcttcttc
    38281 gctatttgaa tgtaaatcag ccactgtgtc gctaaaaaga gacaacttaa gataaactta
    38341 ttagattaca ataaaatcaa tcatttaagt gtcgccagaa agcgacacgg taaaccaccc
    38401 attgtcgtga tttacagaca caaaaaagct cccggagttg ggagcttatg atagtggttg
    38461 gtgcttaatg ccggatgcgg cgtaaatgcc gcatccgggc tgcaaaacca atgaactgat
    38521 gacttacccc aactgcttac gcgcattgcg gaaaatgcgc atccatgggc catcctcgcc
    38581 ccagttttcc ggatgccagg agttgctgac agtacggaaa acacgttccg ggtgcggcat
    38641 cataatggtg actcgaccac tttcagtcgt aaccgccgta ataccgttcg gtgaaccgtt
    38701 cgggttagcc gggtaggttt cagtgacttt gccgaagtta tcgacatagc gcagtgccac
    38761 cagccctttg ctttccagtg ccgccagatg cgccgcatca cgcacttcca cgcgcccttc
    38821 accatgagag acagcaatcg gcatttgcga gcccaccatc ccctgcaaca gcagagacgg
    38881 gctttgggtt acttcaacca ggctgaaacg cgcttcaaag cgatcggagg tgttacgcac
    38941 aaaacgtggc cacaactcac tccccgggat cagttcacgc aggttagaca tcatctggca
    39001 accgttacat acccccaggg ccagcgtttg cggacgatgg aagaaggttg caaactcatc
    39061 gcgtacacgg tcattgaaca ggatagactt cgcccaacct tcaccggcac ccagcacatc
    39121 accgtaggag aaacctccgc acgcgaccag ggcgtgaaaa tcctccagtc ccgtacgtcc
    39181 agtcagcagg tcgctcatat gcacgtcgat agcatcaaag cccccacgtt ggaaagctgc
    39241 cgccatttca acatgcgagt tcacgccctg ctcacgcagt acggcaactt tcggacgtgc
    39301 gccagtggca atatacggtg ctgccacatc ttcgttgata tcgaacgaca gttttacatt
    39361 caggcccgga tcggcgtcgt tagatttcgc ctgatgctcc tgatcggcac actccgggtt
    39421 gtcacgcagg cgctgcatct gccaggtagt ttctgcccac cagacacgca acgtagtgcg
    39481 gctttcgctg aatacagtct gcccgttggc ggtaatcaca aaacggtcac cggaaaccgc
    39541 ctgcccaaca taatggacgc aatccgcaag cccatgctgt gccagtacgg actcgaccgc
    39601 ttcacggtca gcggcacgaa cctgaatcac cgcacccagt tcttcgttaa acaacgccgc
    39661 caggcgatcg tcacccagag aggcgatatc cgcgtcaatg ccacaatgac ctgcaaaggc
    39721 catttccgcc agcgttacca gcaggcctcc atcagagcgg tcgtgatacg ccagcagctt
    39781 acgctgtgca accagcgcct gaatcgcgtc atagaagcct ttcagttgga caacatcgcg
    39841 tacatctgcc ggtttgtcgc caagctgacg ataaacctgc gccagcgccg ttgcgcccag
    39901 cgcgttattg cctttgccca aatcaatcag cagcagtgcg ttatcttcgg tacaaagctg
    39961 cggcgtgatg gtgtgacgta catcttccac gcgggcaaat gcagaaatca ccagcgacag
    40021 cggcgacgtc atttcgcgct cttcgttacc ttcctgccag cgggttttca tcgacataga
    40081 gtctttaccc accgggatcg tcaggcccag cgccggacaa agttcttcac ccacggcttt
    40141 aacggcttca tacaggcccg catcttcacc agggtggcct gccgccgcca tccagttggc
    40201 ggaaagttta atgcgtttga tatcgccaat ctgtgttgcg gcaatattgg ttaacgcttc
    40261 accgaccgcc agacgggcag aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc
    40321 aatcgccatc gcttcaccgt agtagctgtc gaggctggcg gtagtgaccg cgcagttagc
    40381 gaccggcacc tgccacggcc ccaccatctg atcgcgcgcc accatgccgg ttacgctgcg
    40441 gtcaccgatg gtcaccagga aagttttctc cgccacggtc ggcagatgca gtacgcgttt
    40501 caccgcgtct gcaatggtga tcccttcacg gaccagcgca tcgcccttcg ctttcagcgt
    40561 ttgtacatcg cgggtcatct tcggcgtttt accaagcagg acgtccagcg gcagatcgat
    40621 cggctgatta tcaaaatgac gatcgtgcag agaaagatgc agttcttcgg tcgcttcacc
    40681 aatcaccgcg tagggtgcgc gctcacgctt acacagttcg tcaaacagcg gtaattgatc
    40741 ggcagcaacc gccagcacgt agcgttcctg ggattcgtta caccagattt ccagcgggct
    40801 catgcccggt tcgtcgctta gaatatcgcg cagttcaaat ttaccgccgc gtccgccgtc
    40861 gctcaccagc tccggcatcg cgttagaaag accgcccgca ccgacgtcgt ggataaacag
    40921 aattgggtta gcatcaccaa gctgccagca acggtcgatc acttcctggc aacgacgctc
    40981 catctccggg ttgtcgcgct gtacggaagc aaagtcgagg tcggcatcag actgaccaga
    41041 cgccatggaa gacgccgcgc cgccgccaag accgatgttc attgccgggc cgccgagaac
    41101 gaccagcttc gcaccgacgt tgatctcgcc tttttgtacg tgatcggcgc gaatgttgcc
    41161 gatcccgccc gccagcatga tcggtttgtg ataaccgcgc agctcttcgc cattgtggct
    41221 gttcactttt tcttcataag tacggaagta gccgttcagt gccggacgac caaattcgtt
    41281 gttaaacgcc gcaccgccca gcgggccttc ggtcatgatg tccagcgcgg taacaatgcg
    41341 ctcaggctta ccgaaatctt cttcccacgg ctgttcgaag ccaggaattc gcaggttgga
    41401 tacggagaaa ccaaccaaac cagctttcgg cttcgcgccg cgcccggtgg caccttcatc
    41461 gcggatttca ccgccggaac cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg
    41521 gttgtgagtt tcgactttca tcaggatgtg cgccggttcc tgatggaaat tgtagcggcc
    41581 cgtttcgtgg tcagcaaagt agcggcccac ttcagaacct tccattacgg cggcgttatc
    41641 tttataagca gagagaacgt gatctggcgt ggtttcgaaa gtatttttga tcatcctgaa
    41701 cagcgatttc ggctgttgtt caccgtcgat aatccagtcg gcgttaaaaa ttttgtggcg
    41761 gcagtgctca gagttcgcct gagcgaacat atacagctcg atgtcgttcg ggttacgacc
    41821 aagctttgtg aaagcatcct gcagatagtc aatttcatct tccgccagag ccaagccaag
    41881 acgcaggtta gcgtcgatca gcgcctgacg gccctgcccc agcaaatcaa cgcttgtgac
    41941 cggagtcggt tgatggtggg caaacaactg ctctgcatca tctaaatcaa aaaagaccgt
    42001 ttccatcatg cggtcgtgca gttcagcggt aacctgctgc cattgttcat tggtcagcgt
    42061 accggcttct atatagtaag caacgccgcg ctcaaggcgg tttacctgtt gtagcccgca
    42121 gttatgggca atatcggtcg ctttcgaaga ccagggagag atggtgccag gacgcggggt
    42181 caccagcagg agtttgcctt gcggggcgtg gctggcgagt gccggaccat atttcagcag
    42241 gcgttcaagt tgtgcgtgct catcatcgtt taacgacgca ttgaggtcag caaaatggac
    42301 atactcggcg taaatattgt gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt
    42361 gttgattcgg aatgccgaca gtgcaggcga accacgcaga atttccatca taagtctctc
    42421 gtcttctaag ctttcggtgt acccaagggg ggggaaacgg gcgtcattat aaagaatctg
    42481 atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg
    42541 ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt
    42601 atgagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac gacacagaga
    42661 attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct ggcactgctg
    42721 ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa ccgtatcgcc
    42781 gccattcaag cgcggggaga gttgcgtgtg agcaccattc atactcccct gacttataac
    42841 gaaatcaacg ggaaaccttt tggcctagat tacgaactgg cgaaacagtt tgccgattac
    42901 ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca gccagctgtt tgacgacctt
    42961 gataatggta acgccgacct gctggcggca ggactggtct ataacagtga gcgggtaaaa
    43021 aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta taaagtgggt
    43081 cagtatcgcc cacgtacgct gggcaacctg acggcggagc aactcaccgt tgcaccgggt
    43141 catgtggtgg ttaacgatct ccagaccctg aaagaaacaa aattcccgga attaagctgg
    43201 aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga aggaaaactc
    43261 gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca cccggagctc
    43321 gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt ggtttagccc gttagatggc
    43381 gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga agacggtacg
    43441 ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta cgtcgatacg
    43501 cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct gtttgagaaa
    43561 tacgccgaag aaattgactg gcgtttgctg gccgctattg cttatcagga atcgcactgg
    43621 gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac caaaaatacc
    43681 gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag cggtggcgtg
    43741 cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa cgaacggatc
    43801 tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc ccgcgccctg
    43861 acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg tctgccttta
    43921 cttagccaga aaccctatta cagcaagctg acttacggct acgctcgtgg gcatgaagcc
    43981 tacgcttatg tcgaaaatat tcgtaagtat cagattagcc tggtgggtta tctgcaagag
    44041 aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc ggcggtatcg
    44101 cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca gtcgtcatca
    44161 aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga agagaaacaa
    44221 aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga
    44281 aagaagtcac tgagcaacgc cgcgcactca tccgccagta ttccttccgt aatttccact
    44341 cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc gccagttttc
    44401 gcgtcacgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc tccggcacac
    44461 attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata attttgcatc
    44521 accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc atggcgacca
    44581 atcgggcggt tccagccttc gccgattacc cgattgttat gcactaatac cgcgccgacc
    44641 ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg catccaatat
    44701 tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc gcggcgcatt
    44761 atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg cggggttact
    44821 cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc gctatagcca
    44881 ctgtacagcc gcagcggcgt gccgattttg cgctccagtt gtgcgacctt ttcatgtccc
    44941 agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct ggcgataaga
    45001 ttaacccgcg gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag
    45061 ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa tcgctcctga
    45121 accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc
    45181 tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact catcggccag
    45241 cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa cggcaggaca
    45301 agtaacgcat tcagcggttg gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc
    45361 tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg agttgccaaa
    45421 ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag cctaacagat
    45481 agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat ttttaattca
    45541 tggcaattag cggcaatgga atataaaatt cactcgcgtg tgtctcatat tgcctgttgt
    45601 cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg tcagcgttac
    45661 caagggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca acctgatacg
    45721 gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca gtccagcgtc
    45781 gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt ggcgttgagt
    45841 gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca aatccgcggt
    45901 gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac
    45961 gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct
    46021 gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca aaactttgcc
    46081 tggaagctga tgaagattgg cttcaatgct gccgcactgc gcgatatgca tgcgctactc
    46141 gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta caccggtgta
    46201 cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa agtgctggcg
    46261 attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct gtataccatt
    46321 gccgaagaac aagcgacaaa cagtgcttca atctctgctt gtcacgctca gggaatgtta
    46381 acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc agaacgtatt
    46441 cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc
    46501 gcccggtttg tgttgttttg agagtttcct tatggcgctg ttaatcacta aaaaatgcat
    46561 caattgtgat atgtgtgaaa tcaaataaaa acacatttat tttcatagtg atacagcaat
    46621 gtgttattga atgtgttgtt ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac
    46681 acaaagaaca ccactttctt taacgtcctc cgcgccctaa aggacggggc tttacggcgc
    46741 aaaaggcctg tgacatacaa cattaacttt tcagtcaacc gtagcacgtt cttgtatacg
    46801 ccgtgtctgc ggttttatat aaccctggct ggaaacctct tatacaaagt cgattcgcca
    46861 acatcataga tgatcgccac cttctggcga gaaactcctg atgcaattag tcgcccggcc
    46921 tgcgcccatt attctggtga aagtttggga cggcgaccgc caattcgtcc ctgtgcgcga
    46981 gcagcttcca gtcctgcttt tgttcgttca acaatcagtt cacgttccat ttcagccagg
    47041 gcacccatca catggaagaa aaagcgtccc attggtgtgc tggtatcaat tgaatcagtc
    47101 agactacgaa agttgatgcc tcgttcgcgc aactcctcca ccagcacgac aagatgccgc
    47161 atactgcgcc ccagccgatc cagcttccag acaaccagtg tgtcgcctgc cgataatgtc
    47221 ttgagcagtt ttttcagccc cggtctttcg gactttgtac cacttatctt gtcttcaaaa
    47281 atcagctcgc atcctgcaca gttcaacgca ttacgttgta gatcggtatt ctggtcattt
    47341 gttgatacgc gtacatagcc aataagcatg gtaaatcccc ctgttaacaa gacactgcgt
    47401 caaaagtcgg
//
LOCUS       DAJVPP010000018        47134 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVPP010000018 DAJVPP010000000
VERSION     DAJVPP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.018"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..616)
                     /gene="tdk"
                     /locus_tag="OQM34_001026"
     CDS             complement(<1..616)
                     /gene="tdk"
                     /locus_tag="OQM34_001026"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1451567.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1221..1634
                     /gene="hns"
                     /locus_tag="OQM34_001027"
     CDS             1221..1634
                     /gene="hns"
                     /locus_tag="OQM34_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1451568.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(1778..2686)
                     /gene="galU"
                     /locus_tag="OQM34_001028"
     CDS             complement(1778..2686)
                     /gene="galU"
                     /locus_tag="OQM34_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1451569.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(2888..3901)
                     /gene="rssB"
                     /locus_tag="OQM34_001029"
     CDS             complement(2888..3901)
                     /gene="rssB"
                     /locus_tag="OQM34_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1451570.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(3993..4898)
                     /gene="rssA"
                     /locus_tag="OQM34_001030"
     CDS             complement(3993..4898)
                     /gene="rssA"
                     /locus_tag="OQM34_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1451571.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5011..5469
                     /locus_tag="OQM34_001031"
     CDS             5011..5469
                     /locus_tag="OQM34_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1451572.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5519..6361
                     /gene="purU"
                     /locus_tag="OQM34_001032"
     CDS             5519..6361
                     /gene="purU"
                     /locus_tag="OQM34_001032"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1451573.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6521..6605
                     /locus_tag="OQM34_001033"
     tRNA            6521..6605
                     /locus_tag="OQM34_001033"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6555..6557,aa:Tyr,seq:gta)
     gene            6652..6783
                     /locus_tag="OQM34_001034"
     ncRNA           6652..6783
                     /ncRNA_class="other"
                     /locus_tag="OQM34_001034"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6967..7644)
                     /gene="narI"
                     /locus_tag="OQM34_001035"
     CDS             complement(6967..7644)
                     /gene="narI"
                     /locus_tag="OQM34_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1451574.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7644..8354)
                     /gene="narJ"
                     /locus_tag="OQM34_001036"
     CDS             complement(7644..8354)
                     /gene="narJ"
                     /locus_tag="OQM34_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1451575.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8351..9889)
                     /gene="narH"
                     /locus_tag="OQM34_001037"
     CDS             complement(8351..9889)
                     /gene="narH"
                     /locus_tag="OQM34_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1451576.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9886..13629)
                     /locus_tag="OQM34_001038"
     CDS             complement(9886..13629)
                     /locus_tag="OQM34_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1451577.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14022..15413)
                     /gene="narK"
                     /locus_tag="OQM34_001039"
     CDS             complement(14022..15413)
                     /gene="narK"
                     /locus_tag="OQM34_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1451578.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            15752..17548
                     /gene="narX"
                     /locus_tag="OQM34_001040"
     CDS             15752..17548
                     /gene="narX"
                     /locus_tag="OQM34_001040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1451579.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17541..18191
                     /gene="narL"
                     /locus_tag="OQM34_001041"
     CDS             17541..18191
                     /gene="narL"
                     /locus_tag="OQM34_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1451580.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18192..19586)
                     /gene="ychO"
                     /locus_tag="OQM34_001042"
     CDS             complement(18192..19586)
                     /gene="ychO"
                     /locus_tag="OQM34_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS1451581.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            19771..20124
                     /gene="ychN"
                     /locus_tag="OQM34_001043"
     CDS             19771..20124
                     /gene="ychN"
                     /locus_tag="OQM34_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1451582.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20168..20863)
                     /gene="chaC"
                     /locus_tag="OQM34_001044"
     CDS             complement(20168..20863)
                     /gene="chaC"
                     /locus_tag="OQM34_001044"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1451583.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21021..21251)
                     /gene="chaB"
                     /locus_tag="OQM34_001045"
     CDS             complement(21021..21251)
                     /gene="chaB"
                     /locus_tag="OQM34_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1451584.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21521..22621
                     /gene="chaA"
                     /locus_tag="OQM34_001046"
     CDS             21521..22621
                     /gene="chaA"
                     /locus_tag="OQM34_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1451585.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23026..23133
                     /locus_tag="OQM34_001047"
     CDS             23026..23133
                     /locus_tag="OQM34_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1451586.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23282..24136)
                     /gene="kdsA"
                     /locus_tag="OQM34_001048"
     CDS             complement(23282..24136)
                     /gene="kdsA"
                     /locus_tag="OQM34_001048"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1451587.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24172..24981)
                     /locus_tag="OQM34_001049"
                     /pseudo
     CDS             complement(24172..24981)
                     /locus_tag="OQM34_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            complement(24985..25377)
                     /locus_tag="OQM34_001050"
     CDS             complement(24985..25377)
                     /locus_tag="OQM34_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1451588.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25374..26207)
                     /gene="prmC"
                     /locus_tag="OQM34_001051"
     CDS             complement(25374..26207)
                     /gene="prmC"
                     /locus_tag="OQM34_001051"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1451589.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26207..27289)
                     /gene="prfA"
                     /locus_tag="OQM34_001052"
     CDS             complement(26207..27289)
                     /gene="prfA"
                     /locus_tag="OQM34_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1451590.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27331..28587)
                     /gene="hemA"
                     /locus_tag="OQM34_001053"
     CDS             complement(27331..28587)
                     /gene="hemA"
                     /locus_tag="OQM34_001053"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1451591.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            28801..29424
                     /gene="lolB"
                     /locus_tag="OQM34_001054"
     CDS             28801..29424
                     /gene="lolB"
                     /locus_tag="OQM34_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1451592.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29424..30275
                     /gene="ispE"
                     /locus_tag="OQM34_001055"
     CDS             29424..30275
                     /gene="ispE"
                     /locus_tag="OQM34_001055"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1451593.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30426..31373
                     /gene="prs"
                     /locus_tag="OQM34_001056"
     CDS             30426..31373
                     /gene="prs"
                     /locus_tag="OQM34_001056"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1451594.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31498..33177
                     /gene="dauA"
                     /locus_tag="OQM34_001057"
     CDS             31498..33177
                     /gene="dauA"
                     /locus_tag="OQM34_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1451595.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33232..33510)
                     /gene="ychH"
                     /locus_tag="OQM34_001058"
     CDS             complement(33232..33510)
                     /gene="ychH"
                     /locus_tag="OQM34_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1451596.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            33788..34372
                     /gene="pth"
                     /locus_tag="OQM34_001059"
     CDS             33788..34372
                     /gene="pth"
                     /locus_tag="OQM34_001059"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1451597.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34489..35580
                     /gene="ychF"
                     /locus_tag="OQM34_001060"
     CDS             34489..35580
                     /gene="ychF"
                     /locus_tag="OQM34_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1451598.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36424..39291
                     /locus_tag="OQM34_001061"
     CDS             36424..39291
                     /locus_tag="OQM34_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1451599.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39391..41310)
                     /gene="dhaR"
                     /locus_tag="OQM34_001062"
     CDS             complement(39391..41310)
                     /gene="dhaR"
                     /locus_tag="OQM34_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCS1451600.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41538..42608
                     /gene="dhaK"
                     /locus_tag="OQM34_001063"
     CDS             41538..42608
                     /gene="dhaK"
                     /locus_tag="OQM34_001063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCS1451601.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42619..43251
                     /gene="dhaL"
                     /locus_tag="OQM34_001064"
     CDS             42619..43251
                     /gene="dhaL"
                     /locus_tag="OQM34_001064"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCS1451602.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43262..44680
                     /gene="dhaM"
                     /locus_tag="OQM34_001065"
     CDS             43262..44680
                     /gene="dhaM"
                     /locus_tag="OQM34_001065"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCS1451603.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45000..46697
                     /gene="treA"
                     /locus_tag="OQM34_001066"
     CDS             45000..46697
                     /gene="treA"
                     /locus_tag="OQM34_001066"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1451604.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(46776..47071)
                     /locus_tag="OQM34_001067"
                     /pseudo
     CDS             complement(46776..47071)
                     /locus_tag="OQM34_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367998.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11802 a        12156 c        11561 g        11615 t
ORIGIN      
        1 aatcgtggcg atgcctttcc tgaatagccg ttaatgagcc gacttgtaac gcctctttat
       61 agtgtttacg gcatacagaa acgtatcgtt cattaccacc aattaccacc tgctcacctt
      121 cgttataagg tctgcctgct tgatcaagac gcagcaccat gcttgcttta cggccacaaa
      181 aacagatggt ttttaattca accagtttgt cggaccatgc cagtaagtat tggctgccaa
      241 taaataattc acctcgaaaa tcggtacgta aaccataaca aagtacgggt atatcgagtt
      301 gatcgacaac ctccgataat tcatatactt gttgtctggt taaaaactgg cattcatcaa
      361 ccagtacgca atgaattgcc tgctgttcat gttccgcacg aatctcatca aataatgatg
      421 aattttggtt aaataatttt gcaggcgatg acaaacctat acgcgaactg actttcccgg
      481 caccaaagcg atcatcaatt tctgccgtat atacgacagt gcgcatgccg cgttcctggt
      541 aattgtatga agattgcaac aatgctgtag acttacccgc attcattgcg gaatagtaga
      601 aatatagctg tgccatcagc cacaggccct caatgatatg aaataaagtt ggctggagtt
      661 tatcataatt cgtcagtttt tcagctcaac agtatgcaca gaagtgggta tatgcgttct
      721 cccttacgaa gccttgcata atccttctga gctatcatta caactgcctc gcttgttata
      781 agcgggtaaa tgactgctgg taactattca caatctttaa cctgttgcgc aagtaatagc
      841 cctctgttga cctccaggag atagtgcaat actaagtcca tgctcttatt gcgactgttc
      901 tacttttcat cattcgctta atagggaatt ctcgtaaaca caactaatac agaagactga
      961 aaggtcgtca gcctacgata atctccccat aaaatgtgac atgaatcagg aagttttaac
     1021 ctcacgtgct gcgaaatcat cggtgtaaat agggctatat gccgcgtctt ttctggctaa
     1081 ttttatgaaa agatatttat tggcggcaca aaataaagaa caattttgaa ttccttacat
     1141 tcctggctat tgcacaactg aatttaaggc tctattatta cctcaacaaa ccaccccaat
     1201 ataagtttga gattactaca atgagcgaag cacttaaaat tctgaacaac atccgtactc
     1261 ttcgtgcgca ggcaagagaa tgtacacttg aaacgctgga agaaatgctg gaaaaattag
     1321 aagttgttgt taacgaacgt cgcgaagaag aaagcgcggc tgctgctgaa gttgaagagc
     1381 gcactcgtaa gctgcagcaa tatcgcgaaa tgctgatcgc tgacggtatt gacccgaacg
     1441 aactgctgaa tagccttgct gccgttaaat ctggcaccaa agctaaacgt gctcagcgtc
     1501 cggcaaaata tagctacgtt gacgaaaacg gcgaaactaa aacctggact ggccaaggcc
     1561 gtactccagc tgtaatcaaa aaagcaatgg atgagcaagg taaatccctc gacgatttcc
     1621 tgatcaagca ataatctttt gtagattgca cttgcttaaa atcccgccag cggcgggatt
     1681 ttttattgtc cggtttaaga caatttaata agaataagct ataaaaaaac ggcgtcgatt
     1741 gctcaacgcc gtttcgtgga taacaccgat acggatgtta cttcttaatg cccatctctt
     1801 cttcaagcca ggctttaaat tccgtgccaa gggtgttatg acgaataccg tattcaacga
     1861 aggcctgcat gtaacctaat ttattaccgc agtcatggct cttccctttc atatgatagg
     1921 cttccaccgt ttctttttcg atcagcatat caattgcgtc ggtgagctga atttcatcac
     1981 cagctcccgg aggggttttt gccagcaacg gccaaatatc cgcgctaagt acgtaacgac
     2041 ccacaatagc gagattagac ggcgcaacat ccgctttcgg tttttctacc acaccaacca
     2101 tcggtacgct ttcacccggc gctaattcaa cgcctttgca atccacaacg ccatatgcgg
     2161 tcacatcagc aaccggttca accatgatct ggctatgacc cgtttcatca aagcggcgga
     2221 tcatctctgc caggttatcc tgtgacaaat cggattcata ttcatccaga ataacatcag
     2281 gcaaaataac agctaccggt tcatcaccca ctaccgggtg agcacacaat accgcgtgtc
     2341 ccaggccttt cgccagaccc tgacgaactt gcataatagt cacgtgcggt ggacaaatag
     2401 actgcacttc atcaagcagt tgacgtttta cacgtttttc cagcattgct tccagttcaa
     2461 aactggtatc aaagtggttt tcaatagagt ttttagatga gtgtgtaacc agcacaattt
     2521 cagtaatgcc agccgcaata cattcattca cgacgtattg aattaatggc ttatcgacaa
     2581 gtggcagcat ctctttcggg atggctttcg tcgccggcaa catcctggtt cctaatcccg
     2641 caacggggat aacggctttt ttgactttcg tattaatggc agccatttat attctcctgg
     2701 actgttcgtg ttttgaacgt gttcatattt ctgtatcgca tcccagtata tcagcacccc
     2761 gaaggggcta agttctgaaa aggaggcgag atagcataat taagacaatt acttatacga
     2821 atggcgacga tagtaccacc agccgacatt agcaggtaat gcaaatttag cccgcgttat
     2881 cgtttgctca ttctgcagac aacatcaagc gcagtcgacc accggttccc catatttggc
     2941 attgccaggc atcgcatcgc tggctcaatt gattcaaata agcgttacct aaagtgccta
     3001 acggaacacc attactgatt tgcacctggt gttcgccggt atttaacgtc gcattcagac
     3061 ccgcagaaac cagaatgaga tttttcagtt cgcgatgata atagccaact aatagcggaa
     3121 actgccccgg cagattggcc tgacgaagta aatggtttac ctgcttcaat aacgcgccca
     3181 actctggcaa ccgttgattt tggtgtgcaa gctgttcctg taataatccg ttaaacaatg
     3241 cgcgtaataa caaggcagca agtacgccat tatgtccagc tcgggtgaca tcaaggcaat
     3301 aaaatgccag atcgttttcc gaaagtgcag caatatcaag caccaggccg ggtttgtccg
     3361 cggcaaccaa ttgacggtaa ttaacccggc aatgggaaat cacctgctga accggcggtt
     3421 gtagttcctg taataatttc gccgctgcgg cagggttatc aaccattgca tcccagtcgc
     3481 gaaaaagcct ttcctcttcc tcaacgcgcg aattaaacat gctgggatag agacaggcaa
     3541 aaaccatctc gcgcaagcga ttcagatctt taactggttt cagcaaaaca tcttcaacgc
     3601 ccagacgtaa cgctttggca atatctgcca tattttcagt ggcagatatc accagaactg
     3661 gggtctggtc gcctctgtta cgtatatgct ccagcagttt aagcccgttc attcgtggca
     3721 tcgcgatatc acatatcatc aggtctggag tgaaacctcc cagcaactca agggcatcca
     3781 ccccatcagc cgccagtacc gttgtcgctc ccaatgagga aaaccatgaa tccagaagcg
     3841 agcgaaatac ctgctcatct tcaacaatga gaatctgttt tccgaccaat ggctgcgtca
     3901 tgttctctcc cctgactggc tttactcaat agtggcatgc tattgccacc tgcgcctgtc
     3961 agaattaact taagtgtaga taaaaattct ggtcaaatgt tggtgcgtac caacggcaaa
     4021 agttcgtcca ttttcctttc cactgccagc tgtccggccg caatggcagc gtgcgcgcga
     4081 tggaaatcaa gcgtagatat ttgcgggcaa acaggttgaa tcagaatatc gggcggatca
     4141 cctgccatgc ggttcctttt aaggcggttc tccaacacct ggatagacgt ggtcataatc
     4201 tctgttgccg ttggcgctgt cacagcccga cgtgtcgtta tgctgcctaa cctttctttc
     4261 agacgcgcat gccacggtag agaatcacca ttctcgctat tttcttcact gacattaaag
     4321 gagagcagat cttgttgcat caaatgagca tcgtgctgca ggtcgaccgc tatcacaata
     4381 tcagccccca atgcacgcgt aagagaaata ggaattgggt taactactgc cccatcaacc
     4441 agccagtagc cgttatgtgc aacgggtgcc atgagtcctg gaatactgca tgatgcgcga
     4501 atagcaagat ggagatcgcc ttcagtaaac cataattcac gccccgtact taaattggtg
     4561 gcaacagccg caaagcgacg ggaacaattt tcgatctctg tttccggcat tatttcgcga
     4621 tattgattga agacacgctc gccgcgcagt aacccaccgc gctgccagga gaggtccatc
     4681 aggcgtaaaa cgtcccaata actgaaagag gttacccaat cttccagcgc agataatcga
     4741 tcgcatgcat aggccgcgcc caccagcgaa ccaattgaac atcctgcaac gatatcaatt
     4801 tcaataccca ctttttttag cgcattaata acgccaatat gcgaccaacc tctcgccgcg
     4861 ccagatccca gcgccagccc tatttttatc tttctcatta ttcccgcaag attcccctgg
     4921 aatgcaatcg tagccacatc gctactgctc ggttaacata gtgctactca ggcggttaat
     4981 acgccgttat tgtttccagg gagattattt gtgtctcagc tttgtccctg tggtagtgct
     5041 gtcgagtata gcctatgttg ccacccttat gtgtctggtg aaaaggttgc acctgatcca
     5101 gaacatctca tgcgttcgcg ttactgcgct tttgtgatgc aagacgctga ttatttaata
     5161 aagacctggc atccctcttg tggagcagcg gcgttacgtg ccgaattgat gaccggattt
     5221 gcgcatactg aatggttggg attgaccgta tttgaacatt gttggcagga cgccgacaat
     5281 atcggatttg tcagttttgt tgctcgattt accgaaggtg gcaaaacagg tgcaataatt
     5341 gaacgttcgc gatttttgaa agaaaacggt caatggtact atattgacgg tacacgtccg
     5401 cagtttggtc gcaacgatcc ctgcccttgt ggttcaggta aaaaatttaa aaagtgctgc
     5461 ggccaataat ggttgacggt acggtttagc aaacactctc aacaaggttt ttccagcaat
     5521 gcattcactc caacgtaaag ttctgcgtac tatttgtccg gaccaaaaag gtctgatcgc
     5581 acgtattacc aatatttgct acaagcacga gttaaatatc gtacagaaca atgaatttgt
     5641 tgatcaccgt accgggcgct tttttatgcg cacggaactg gaagggattt ttaatgattc
     5701 caccctgctg gcggatctcg atagcgcatt gccagaaggc tccgtgcgtg agctgaatcc
     5761 tgccggtcgt cgccggatag tgattctggt cactaaagaa gcgcattgcc ttggcgattt
     5821 gttgatgaaa gccaattacg gcggtctgga tgtcgaaatc gcggcagtta ttggtaacca
     5881 cgatacttta cgttctctgg ttgagcgttt tgatattccg tttgagctgg taagccatga
     5941 agggttaagc cgcaacgagc acgatcaaaa gatggcggac gccattgatg cttatcaacc
     6001 tgactatgtg gtgctggcga agtatatgcg ggtattaacg ccggaatttg tggcacgctt
     6061 cccgaataaa atcatcaata ttcaccattc attcctgcct gcgtttatcg gcgcacgtcc
     6121 ttatcaccag gcctatgaac gtggtgtgaa gattattggc gcaaccgctc actatgtgaa
     6181 tgacaatctg gacgaaggcc caatcatcat gcaggacgtt attcatgtcg atcataccta
     6241 cacagctgaa gatatgatgc gcgcaggtcg tgacgtcgag aaaaacgtct taagtcgtgc
     6301 gctatacaaa gtactggcgc aacgcgtctt tgtttacggt aatcggacga ttattcttta
     6361 atcgccagca aaaataactg gttaccttta atccgttacg gatgaaaatt acgcaaccag
     6421 ttcatttttc tcaacgtaac actttacagc ggcacgtcat ttgatatgat gcgccccgct
     6481 tcccgataag ggagcaggct agtaaaaagc attaccccgt ggtggggttc ccgagcggcc
     6541 aaagggagca gactgtaaat ctgccgtcat cgacttcgaa ggttcgaatc cttctcccac
     6601 caccatcact ttcaaaagcc ccggaattct caaacgaatc tgcaatcaaa tattctgccc
     6661 aagcggggaa ggatgagaag cttcgaccaa ggttcgactt gagcgccagc gagagagcgt
     6721 tgccgcaggc aacgacccga agggcgaagc gcgcagcgct gagtaatcct tcccccacca
     6781 ccatcgcttc cttgaataat caaaacctgt tactccgagc taattttgcc cgatggcacc
     6841 acgtttaccg ggcctacaat atcctcccct gtaggtcgga taagcgaagc gcatccgaca
     6901 actcaccccg caccgtgccc ataaaaaaac cccgccgaag cagggtctat gtgaactaaa
     6961 attcgtttag tgacgagcgc gcaccagctg gtacttacgt gtcagatact ccaccggtac
     7021 gctccagatg tgtaccagac gcgagaacgg gaacagcagg aataacgtca tccccagcac
     7081 caggtgcaga cggaagataa acgccacacc atcgaggtgt tgagaagcgc caccgtggaa
     7141 ggtcaccacc gactgcgccc agccaaccag tttcatcatc tcgctaccgt ccatatgctg
     7201 agcggagaac ggaatggtca gcaggcccag cgcgcactgg ataacgagca gcgacaggat
     7261 caggatatcc gctccggtag tggttgcacg cacgcgtggg ctgaacatac gacgtttcag
     7321 caaaagcacg ccgccaatca gacacagcac gccgctggca ccaccagcaa acatcgccat
     7381 tttctgtttc acttcaatcg gcagccaggc ttcatacatc cagtgcggcg tcaacatacc
     7441 gaagaagtga ccgacaaaaa tccccagaat accgatatgg aacaggttcg acgccaggtt
     7501 catccctttg cgatccagca tctggctgga cgccgcgcgc caggtgtact gcccgtagtc
     7561 ataacgcagc cagctaccaa tcaggaagac cgccccggct atgtacgggt agatatcaaa
     7621 gaagaacata ttcaggaatt gcattagtgc tgtcctccgg tggtgatatt cagatattgc
     7681 ggcgcgacgg caccggcaaa gcgacgctga tgagcagtga ttgctgaatc accgcagcct
     7741 ttgtcagcaa agaatttaac ctgctcttct tcccagacag catccagcgc ctgcggcgta
     7801 tcatcgcgcg cttcgtcggc aattttttcc gccactttgt cgctgtcgat agcggtattg
     7861 gccaatttca gcagcagatc aaacatcacg gcataacggc tttcacgctg ttgcagacgc
     7921 gcgctcagca atgccagaat cggcgcgata tctttcaaac cttccacggc ttcgctttgc
     7981 ggcagctgcg acaggtactc cagatacagc ggcagatggt ccggtaattc gcggctgttt
     8041 aactgcaagc cgtgctgctc gtactgcgcc agcaggtcca ccatcgcctg gccgcggtca
     8101 cgggattcac cgtgaacatg ttcaaatagc aacagtgacg tggcgcggcc acggtcgaac
     8161 agttcgctgt actggacctg ggcatcgagc ggatccatcg ccgttaaatc gcgcaggaaa
     8221 atgcccagcg catgggcatc ttctttcgac agatttttcg acgcagcaat cgcctcaaac
     8281 atctcttgtt gatgctgcca taaggcagca tccggatatt caaggagacg cgatacaatc
     8341 acgagttcga tcatggatgc ggctccgttt tgctggtcac atcgatggca tcgatacgac
     8401 ggctgttgaa cagattgaat ttggtatctg agccgtggca gccatcacca aaggtaaagc
     8461 cgcagccatt tttctccggg aaggcttccc gtgccagttc acgatgacta ctcggcacca
     8521 caaagcgatc ttcgtagtta gcaatcgcca gataacggta catctcctgt gcctgggctt
     8581 cggtcagacc gacctcttcc agcgcacggg tatcaacttt accgtcaaca gtttcagcac
     8641 gtttgtaatg acgcatcgcc agcatacgtt tcagcgcacg cagtaccggt ttggtatcac
     8701 cggcggtcag cagattcgcc agatactgta ccggaatacg caagctttcg acgtctggca
     8761 gaattccgtt gctgcccagc tcgcccgcgt ctgctgcaga ctgaatcgga gacagaggcg
     8821 gcacgtacca gaccatcggc agtgtgcgat attctggatg cagcggcagc gccagtttcc
     8881 attccatcgc cattttataa accggcgact gctgtgcagc ttcaataacg ctcagcggaa
     8941 taccgtcttt aatcgcctgc tcgatgactt tcggatcgtt cggatcgagg aacacgtcca
     9001 gctgacgctg gtagagatct ttctcgttct cggtgctggc tgcacgttca atcgcgtcgg
     9061 catcgtacag cagcacgcca agataacgga tacgaccgac acaggtttct gagcacacgg
     9121 tcggctgacc cgcttcaata cgcggatagc agaagatgca cttctcagac ttaccgctct
     9181 tccagttgaa gtagattttt ttgtacgggc atccggtgat gcacatacgc cagccacggc
     9241 atttatcctg gtcgatcagg acgatgccat cttcttcacg cttgtaaatc gcaccgctcg
     9301 ggcaggtcgc cacacatgcc gggttcaggc agtgttcgca caggcgcggc aaatacatca
     9361 taaaggtgtt ttcgaactgg ctatacatcg ccttctggat gttgtcgaag ttcttgtctt
     9421 tcgccagttt atcaaactca ccgcccaggt catcttccca gttcggccct ttttcgattt
     9481 tcgccatccg ttccccggta atcagcgaac gcggacgggc aatcggctgc gatttgctgc
     9541 cttccggcgc ggtatgcagg ttctgataat caaaatcgaa cggctcgtaa taatcgtcga
     9601 tccccggcag atgcgggtta gcaaagattt tacccagcag catggctcgg ttacccatgc
     9661 gcggttgcag tttgccattg attttacgga tccagccgcc tttgtatttt tcctggtttt
     9721 cccagtcagt cgggaagccc tggcccggct tggtttccac gttgttgaac cacgcgtatt
     9781 ccacgccttc acggctggtc cagacgtttt tacaggtaac tgaacaggtg tggcagccga
     9841 tgcacttatc gagattcagc accatgccga cttgtgaacg aattttcatt ttacgctctc
     9901 ctgtacctgg tcattgcctt cgccatctaa ccagtcaatg ttcttcatct tacgcactac
     9961 aacaaactca tcgcggttag aacctacggt gccgtagtag ttaaagccgt atgccagatg
    10021 ggcatagccg ccgatcatat gcgtcggttt cggcgtgata cgggtgaccg agttatggat
    10081 accaccacgc tgttgggtaa tttccgaacc aggcaggtta acgatacgtt cctgcgcgtg
    10141 gtacatcatg gtcatccctg ccggaacacg ctggctgaca accgcacggg cagtcagtgc
    10201 accgttgctg ttgaagactt caatccagtc gttatcggcg atacccagat ctttggcatc
    10261 ggcttcactc aaccagacca ccggaccacc gcgacctaaa gtcagcatca gcaggttgtc
    10321 gctgtaggtg gagtggatac cccacttctg gtgcggcgtc aggaagttga gcgctttttc
    10381 cgggttgccg ttggatttct ggcctatcac ttctttcacc gaacgggtgt cgatcggcgg
    10441 acgataaacc agcaggcttt caccgaaatc acgcatccac tggtgatcct gatacagttg
    10501 ctgacgacca gagagcgtac gccatgggat cagctcgtga acgttggtgt aaccggcgtt
    10561 gtaagaaacg tgttcatctt ccagaccaga ccaagtcggg ctggagataa ttttgcgcgg
    10621 ctgtgcctga atatcgcgga agcggatctt ctcgtcttct ttattcagcg ccagatgcgt
    10681 atggtcacga ccggtaaatt cgctcagggc agcccaggct ttcacggcta cctgaccgtt
    10741 ggtttccggt gccagcgtca ggatcatctc tgccgcatca attgcggtgt tcagcatcgg
    10801 ctggcctttt gccggacctt ccgctttggt gtagttgagc ttacgcagca gatccatctc
    10861 gctctgggtg ttccaggcaa tccctttacc gccgttaccg attttctcca tcagcgggcc
    10921 gatagaggta aagcgttcgt aagttgccgg ataatcgcgc tctacgacca taatgtgcgg
    10981 tgcagttttg cctgggatca ggtcgcattc gccttttttc cagtctttca catccagcgg
    11041 ctgcgccagt tcagcggcag agtcgtgctg gataggcagc gtgacgatgt cggtttcttt
    11101 acccagatag ccgacgcaca cttcggagaa tttcttcgcg atggctttgt agatttccca
    11161 gtcgcttttc gcttcccagg ccggatcgac cgccgcagac agcgggtgaa taaacggatg
    11221 catatccgaa gtattcatgt cgtctttttc gtaccaggtc gccgtcggca aaatgatgtc
    11281 ggaatagagg caggtgctcg acagacggaa gtccagcgta accaccagat ccagcttgcc
    11341 ttccagacca ttgtcctgcc agtccacttc ttccggcttc acgccgccct gttgccccag
    11401 atctttaccc tggataccgt gctccgtccc cagcaggtac ttgagcataa actcatgacc
    11461 tttaccggaa gaaccgagca ggttagaacg ccagatgaac aggttgcgcg ggtggttttt
    11521 accgttttct ggttgttctg ccgcaaaacg gatggaaccc tctttcaggg atttcaccgt
    11581 atagtccacc ggattcatcc cggctttttc cgcttcgcca gcgatagtca gcgggttagt
    11641 gcctaactgc ggtgcagacg gcagccagcc catgcgttcc gcacggacgt taaagtcgat
    11701 caagtgtccg gtatagcggg atttgtccgc catcggtgac agcaactctt ccgccgtgac
    11761 ggtttcataa cgccactggc tggagtggtt atagaaataa gaagtgctgt tcatgtgacg
    11821 cgccggacgc tgccagtcaa gggcaaacgc cagcggctgc cagccggttt gcggacgcag
    11881 tttttcctga cctacatagt gcgcccagcc gcccccgctc tgaccgacac agccgcagaa
    11941 aatcagcatg ttgatcagac cacgatagtt catatcgagg tgataccagt ggttcagccc
    12001 cgcaccgacg ataatcatcg aacgaccgtg cgttttatca gcgttatcgg caaattcacg
    12061 ggcgatgcga ataatctggc tgcgagaaac gccggtaatc tgctcggccc aggccggggt
    12121 ataagctttc acatcgtcat agctggttgc acagttaacg tcgttcaggc cacgttccag
    12181 accgtagttt gccagcgtca gatcataaac ggtggtcacc agggcggtgc tgccatcagc
    12241 cagttgcagg cgtttcaccg gcagtttgtg cagcagcacg ttttccagtt ccactttgtt
    12301 gaagtgttcc gtgccgtcgc caccaaagta cgggaagcct acctcagcga tctcatcctg
    12361 gctacccagc aggctcagtt gcagctcggt ttcttcgcca gttttgccgt cgcgctgctc
    12421 aagattccat ttgcccttct cgccccagcg gaagccaata gaaccgttcg gcgcaaccat
    12481 ttcgccattg gtattaaagg cgacagtttt ccattccgga ttgttttcct ggcccagcgc
    12541 atcaaccaga tcagcagcgc gcagcatacg acctgcagcg tagtaaccgt cgcgttcttc
    12601 cagcatcacc agcatcggca tgtcggtgta gcgacgcaca tagtcggtga aatactggct
    12661 tgggttgtcg aggtggaatt cacgcagcat tacgtggccc atcgccagcg ccattgccgc
    12721 atcggtgccc tgtttcggtg ccagccacag atcgcacagt ttggcgattt cagcgtagtc
    12781 tggtgtgacg gcaacagttt tggtgccttt gtaacgcact tcagtaaaga agtgagcatc
    12841 cggggtacgc gtctgcggca cgtttgaacc ccaggcgatg atgtagctgg agttatacca
    12901 gtcagcagat tccggtacgt cagtttgctc gccccaggtt tgcggagacg caggaggcag
    12961 gtcgcagtac cagtcgtaga agcttaagca agtaccgcca atcagcgaga gatagcgtgc
    13021 acccgatgcg taagaaacca tcgacattgc cggaattggc gagaaaccag caacacggtc
    13081 cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg atcagttcat tcacctcctg
    13141 ccaagaagaa cgaacaaatc caccgcgtcc acgcgcctgc ttaaagcttt tcgctttatc
    13201 ggcgtcttca atgatagaag cccatgcctc aaccggatcg ctatgcagcg ccttcgcttc
    13261 acgccacatt ttcatcaggc gtttgcgcat catcgggtat ttcaggcggt tggcactgta
    13321 aagataccag gagtagctgg caccgcgcgg gcagccgcga ggttcatggt ttggcagatc
    13381 cggacgggta cgcggatagt cagtctgctg ggtttcccag gtgaccagac cgtttttgac
    13441 gtagattttc cagctgcagg agccggtgca gtttaccccg tgggtagagc ggacgatttt
    13501 gtcatgctgc caacgctggc gatatccatc ctcccagtca cggttggtat tgagaagctg
    13561 gccatgccca tcggcaaagg tttcaccctt ctgtttgaag tagcgaaacc ggtccaggaa
    13621 tttactcatc ggttttctcc tgtgggagcc tgtcggccct ctgataaatc gacattgcta
    13681 atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa ttgataacga tcaagagtga
    13741 tggggatgaa aaataaagta aattcctaag ataccccttt aggataatta acagttacag
    13801 cataacatgc tgtaaattaa gacgttttat acctgtagtt atttaaacta ttaagatttt
    13861 tatgcaaatg ggtattaagg agtattcccc atgggtaaca tattaatggc tcttaatgaa
    13921 atccggcgaa ataagccata taacactaat atgttgtaac taaatgacgc ataaaaaaag
    13981 cgcggcatca tgccgcgcaa aggataatca aatattaaga tttatttttt agaatgccga
    14041 ccatataccg cccaagtaat cactacgcag gcgatataga aaatcaaaaa tactttcatt
    14101 gcacctactg gcgaacccgt taatgccagc gagctaccaa acgctttcgg gataaagaag
    14161 ccaccaatcg cgccaatcgc agagatgaaa cccagcgccg ctgccgtgcc ggttgccgct
    14221 tcacgcatcg cacgttcgtc agaaccccct tctgctttca cgcgatccat tgtcagttta
    14281 cggaagatca ctgaaatcat ctggaaagtg gaaccactac ccagcccagc tgtcaggaac
    14341 agcgccagga agaccgcgaa gaacgccatg aagcttccgc cctgcccgtc agtcggtaag
    14401 gtcaggaaca gcaggccgct gaaaatcgcc atcagaataa agttcaccag cgtgacacga
    14461 gttccgccca gacggtcaga taatgcacca cctgcagaac gcgccagcgc accaataaac
    14521 ggcccgaaga aagcgtattg cagaatctga acatccggga actgcgtttt tgacagcatc
    14581 gcaaagcccg cggagaagcc gatgaaggag ccgaaggttg ccagatacag caggctcata
    14641 atccacagat gaccccgttt gagtaccggc aactgctcct tgatggaggc tttcgaggta
    14701 gcaagatcgt tcatgccaaa ccacgccgca atggtgaaga tggcaaggaa cggcacccat
    14761 atccaggacg cattcgccag atacagctca gtcccatccg gctgtttgac gccctggcta
    14821 ccaaatactg cgaaaatcga cagtgatacc accagcggag caaccaactg catgacgctg
    14881 acgcccatgt ttcccagacc accattcaga cccagcgcgc caccctgctt ctgtttcgga
    14941 aagaagaagc tgatgtttgc catactggat gcgaagttcg cgccagcaaa gccgcatagc
    15001 agagagatga tgatgaagac gctataaggc gtggaggtat cctgcacggc aaaacccagc
    15061 cagacgcaag gaataatcag aataccggtg ctgaacgccg tccagcgacg accaccgaag
    15121 ataggaacca taaaggagta tggaacacgt aataacgcgc cagaaaccga aggcagcgca
    15181 gtcaacataa atagctgatc ggtcgtaaaa ttaaaaccga ctttcggtaa gttcaccgca
    15241 acagcgctga acaacatcca tacgcaaaac gccagcagca gacagggaac ggaaatccac
    15301 aggttgcggc tggcaatacg ttgaccgcgt tgttgccaga acgcaggatc ttccggtcgc
    15361 caatctgtta tgacagctcc agtagccctt tcggaggcag atgagtgact catagacacc
    15421 tctgatactc gtttcgatgt ctgccacctt agtgtctgga gctaaaggca atttgatgta
    15481 aatcaaacga taagataact ttatctatca ttgatattta tcattaccca tagtgagtac
    15541 agtgatttca taaaaattat gagatttttc acggtgctgt agaatcccta cccttaccga
    15601 tgtaaagcga ctaaccacac ggcaaataag gagtaactct ttccggctat gggtatactt
    15661 cagccaatag ccgagaatac tgccattcca gaatatatcg tcacattcat taaggttatt
    15721 gctcatttaa agcctgaagg aaggggttta catgcttaaa cgttgtctct ctccgctcac
    15781 cctggttaat caggttgcgc ttattgtgtt gctttctact gctattggac tggcagggat
    15841 ggcggtttct ggctggctgg tgcaaggcgt tcagggcagc gcccatgcga tcaacaaagc
    15901 gggatcgctg cgcatgcaaa gttaccgtct gttggcggca gtgccattaa gcgagaaaga
    15961 caagccctta attaaagaga tggaacaaac ggcatttagc gccgagttga ctcgagcagc
    16021 agaacgagac ggacaactgg cgcaattaca gggtttacaa gattactggc gtaatgaact
    16081 gatccctgcg ctgatgcgtg cacaaaaccg agaaacggtg tcagcggatg tcagccagtt
    16141 tgttgccggg cttgatcagc tggtatctgg ttttgaccgc accacggaaa tgcgtattga
    16201 gacagtggta ctggtccatc gggtaatggc ggtatttatg gcacttttac tggtgttcac
    16261 tattatctgg ttgcgggcgc gactgctaca accgtggcgg caactgctgg caatggcgag
    16321 tgccgtcagc catcgcgatt ttacccaacg cgcaaacatc agcgggcgca acgaaatggc
    16381 gatgcttgga actgcgttga acaatatgtc tgcagaactg gccgaaagtt atgccgtact
    16441 tgagcagcgg gttcaggaga aaaccgccgg gctggagcat aaaaatcaga tcctctcttt
    16501 tttatggcag gctaaccgcc gtttgcattc ccgcgccccg ctgtgtgaac gcctgtcacc
    16561 ggtactcaac ggcttacaga atttaaccct gctacgtgat atcgaactgc gggtgtatga
    16621 cactgatgat gaagagaatc atcaggagtt tacctgccag ccagatatga cttgtgatga
    16681 taaaggctgc cagctctgcc cgcgcggcgt attacccgtt ggtgatcgcg gcacgaccct
    16741 gaagtggcgg ctggctgact ctcatacgca gtacggtatt ttgctggcga ccctgccgca
    16801 ggggcgtcat cttagccatg atcaacaaca actggtggat accctggttg aacaacttac
    16861 cgccacgctg gcgctggatc gccatcagga acgtcagcaa cagttgatcg tgatggaaga
    16921 gcgtgccacc attgcgcgcg aactgcatga ttctattgcc caatctctct cttgcatgaa
    16981 gatgcaggtg agttgtttac agatgcaggg cgatgcgctg ccagaaagca gccgcgaact
    17041 gttaagtcag atccgtaacg aactgaatgc atcctgggcg cagttgcgtg aattgctcac
    17101 cacattccgc ttgcagctca ccgagcctgg attacgtccg gcgctggaag cgagttgcga
    17161 agagtacagc gccaaatttg gcttcccggt gaagctggat tatcaattgc cgcctcgtct
    17221 ggtgccttca catcaggcaa tccacttgtt gcaaattgcc cgtgaggcat taagtaacgc
    17281 cctcaaacat tcgcaagcga gtgaggtcgt ggtgacggtg gcgcaaaacg ataatcaggt
    17341 caaactgacc gtccaggata acggctgcgg cgtgcctgaa aatgccatcc gcagcaatca
    17401 ctacggcatg ataataatgc gcgaccgtgc gcaaagttta cgaggcgatt gccgcgtccg
    17461 ccgtcgtgaa tcaggtggca ccgaagtggt ggtcaccttt attcccgaaa aaactttcac
    17521 agacgtccaa ggagataccc atgagtaatc aggaaccggc tactatcctg ctgattgacg
    17581 atcacccgat gctgcgaact ggcgtaaaac agcttatcag tatggcacca gatatcaccg
    17641 tggttggcga agcgagtaat ggcgaacagg gtattgaact ggcggaatct cttgatcccg
    17701 atctgatcct gttagatctc aatatgcccg gcatgaacgg tctggaaacg ctggataaac
    17761 tgcgcgaaaa gtccctctca gggcgcattg tggtattcag cgtctctaac catgaagaag
    17821 atgtggtcac cgcactgaaa cgcggcgcgg atggctatct gttaaaagat atggaaccgg
    17881 aagatctgct gaaagcattg catcaggcag ctgctggcga aatggtatta agcgaagcat
    17941 taacgcctgt tctggccgcc agcttgcgcg ctaaccgtgc cactactgag cgcgatgtta
    18001 accagttaac cccacgcgag cgcgatattc tcaagctgat tgcccagggt ttgccgaaca
    18061 agatgattgc ccgccgcctg gatatcaccg aaagcacagt aaaagtgcac gtcaagcaca
    18121 tgctgaagaa aatgaagctc aagtctcgcg tggaagcagc ggtatgggtg catcaggagc
    18181 gcattttctg attacggttc ccagcgcagt tcgtcgtttg acaatgcatc gaacggttcg
    18241 acaagcgtta gcgtgatctc attggaggag acacgctgcc cctggttatc ttccaccacc
    18301 acagacaatc gccagtgatt gcttgcccct tccccgttct gccagtcagg catgatcagc
    18361 gtccagccct ccgcgctgtt ggcttgtgcg cccggcgtca aacttaatat ctgcgtatcg
    18421 ccctgccaaa tcagttgccg aataccgtaa cgactgcgga tttgtaattt cagcggcact
    18481 gtttcgccag gttttagatc ccacggcggt gtcgccagaa acaccgttaa cgtttttcgc
    18541 tgtcggtact caagagtcgg tagattattt cgctgcggat tgtcatagcg actaccacgt
    18601 aacgactgac tttcggcaac ctcgcccgca gaaagttgtt ttttgagcgg tacaccaaag
    18661 cggtaattaa gattcagccc gaggttattt tgattttcgc cactttcccc ctgtttatgc
    18721 tgggccgtca cagtgactaa tggcacaggg gtgtaattta atcccagact caacgcgacg
    18781 ggattgtgat aacccgtacc agagttaaac aaatcaacac gatcaccaaa atactgttct
    18841 aggctgacac tggtattgag gtgttgatag aacggcatgc gcatccgagc tgtcaggtcg
    18901 tacccgcgcg ccatccgttg ttcctgcgtg gctgtctgtt catgccatgc agcaaacggc
    18961 tgataaaagt ttgccgataa tcgcaaatat tcgccccacg cttcggcacc aaagcccgct
    19021 cgctgaagat tttcgtcctg caagttgtca taaaaagtgt tataacccac cagccagttg
    19081 ccgcgcgccc agcgttgccc aacgcccaca ttgctcacca acccattatc ctgctgagta
    19141 agaccaagct ggctccaggt gagataacga tcattatctt gtaacggcac aaaccagctt
    19201 ccacgactgc cggtgaaatg tccttcgtta tccactttga cgtcaacact ggcatttccc
    19261 cacggtgata gccaggactc tacgtgctga ttaacctgtt gactaagcgc gtcgcggact
    19321 tttcccaatg cgaaagcttt tgcctgctcg ccagtatcca gcccgttatc attcatactg
    19381 gtttcgccga aatctttcac aatttcagca aagtgttttt ccccatcatg attttcggga
    19441 gccatgccta aatccggcag accatcattg ttattatcaa agggatttgc cgctttttgc
    19501 tcgaaggtag attgtgcgtt agctgtaccg cctgctacaa gcaagagtaa ataaaacggg
    19561 ataatgcgag gaacgaaacg gctcaaaacg tcggtaactt ccggtaataa tgaaaaacca
    19621 tagccttaaa gaataacgat tactggcaac ttttgtcgta ttttctcgcg ctttcaggaa
    19681 aatccttaaa aatgtgaccg attggtcaga cgctgcagaa gcagtaagat agaagatgag
    19741 tgtctaattc cctgaataca ggagtaaata atgcaaaaaa tcgtgatcgt tgccaatggc
    19801 gcaccttacg ggagcgaatc cttgtttaac agcttgcggc tggccattgc gttacgagag
    19861 caggagagca atctggatct gcgtctgttc ctgatgtctg atgcggtcac agccgggttg
    19921 cgcgggcaaa aaccagggga aggctacaac attcagcaaa tgctggagat ccttaccgct
    19981 cagaatgtac cggtgaaatt gtgcaaaacc tgtaccgacg ggcgcgggat tagcacactt
    20041 cctctgattg atggggtgga aatcggtact cttgtggaac tggcgcaatg gacgctgtca
    20101 gccgataaag tgctcacatt ttaatgttta agggggcgtc tgacgcccca ctatgaggga
    20161 agtttactca ggcgaatccc ggccttaaca caccatccgg ataattctcc gccagtagtt
    20221 tttttaccga taccagcaaa tcattcagcc catcgtcctg cattcccagt ttgataagct
    20281 cctgttccag tgaaaacagg tattgtgcat tggttcccag cggaccgctc gccgccgcaa
    20341 tcaacggcgc gatgacctga gcgcgagtgt cagattcata ttctggatgt cgcgggtcca
    20401 taataaacac aatggcgttt actgtgcgtc catcatcaag atcaagctga caccaggttg
    20461 gcagataaca gccggtaatc atctcgcgct tccacaacag ggttagttcc tgctccagcg
    20521 tctcttctgg tagtcgatag gcgacgcctg tggtgcgtcc gccctctttc agtgcaagca
    20581 tccgtcccgg ctggtgcgca gtcccgcgcc cggcggtcag gcgcaggcaa aacgcgcgat
    20641 gccatccaac cagtgtaccg gtgcatgatt cggtaaactc cagtgccgga ttccacatca
    20701 acgatccata accgaagatc cacaccggtc cctcatcagg tcgacaagcc agcgtcgccg
    20761 ccagcgaagc cgcccgttgt tctgctgacc ataagagtga ttcttcaatg gcaccaaatg
    20821 ccgttttaca atcggcattc atcaagaaat cacgcgttat caccttacac ctccgccact
    20881 gcatacttcc ctgcgacaac tttttggtgt cttccggaca tttttaccgc tcattttgaa
    20941 accactaacg gaacaatatg caatccacgg accacttgca aggcaacacc gcacgaatag
    21001 ctttaactaa gctaccggtt ttacgatttt ttatgccatt tatcatcatc ccctttggca
    21061 tattcatgct tcacagcagc ccaggccact ttatgcgcgg tttcttcgcg actggcgtca
    21121 tcacgccgat cttctttatc tttatattga tcccatgcgc tgttgaacgc ttctttatag
    21181 atatcctggg catgagacgg tagaacgtgc tttacgcttt ccggcagatc gcttttcgtt
    21241 ttatacggca tcgttgactc cttttacaaa gttgaacatt aagtgtggtc aacaatatct
    21301 actcttgcca aaacataaga attttcatgt cagactgttc ttaagaaacg ctaaaacaaa
    21361 cattgtctaa acgtaatact tatttttatg ccccaatgag taaaaaatcg acagcggggt
    21421 aaaaattcgt aaaatgttac cgatatccag acactttctg ttatttttcc tacctatttt
    21481 ttacattttc gataaattca tctgcaaagg gataaccata atgtcaaatg ctcaagaggc
    21541 ggtaaaaacc cgccacaagg agacttcgct tattttcccg gttctggtgc tggtagtgct
    21601 gttcctgtgg ggaagtagcc agacactacc agtggtcatt gccatcaatc ttcttgcgct
    21661 tattggtatt ttaagtagcg cctttagtgt tgtccgtcat gcggacgtat tagcccatcg
    21721 cctgggagaa ccttacggtt cgcttattct tagcctttca gtggttattc ttgaagtcag
    21781 tttgatttca gctttaatgg caaccggcga cgccgcgcca acgctaatgc gtgatacgct
    21841 ctattcaatc attatgattg ttaccggtgg gctggttggc ttttcattat tgttgggcgg
    21901 tcgtaagttt gccacccaat atatgaatct gtttggtatc aagcagtatt taattgcgct
    21961 gttccccctg gcgataatcg tactggtatt tccaatggct ctgcctgcgg cgaatttttc
    22021 aaccggtcag gcgttactgg tagcattaat ttctgcggca atgtatggcg tatttttgct
    22081 gatccaggcc aaaacgcatc aaagtttgtt tgtctacgag cacgaagatg acagtgatga
    22141 cgacgacccg caccacggta aaacgtctgc ccatagcagc ctgtggcatg ctatctggtt
    22201 gattatccat ctgattgccg ttattgcagt caccaaaatg aacgccagct cgctggagac
    22261 attgctcgac agcatgaatg cccctgttgc ctttactggc ttcctggtgg cactgttgat
    22321 tctgtcgccg gaaggtttag gtgcgttaaa agcagtgttg aacaaccagg ttcagcgcgc
    22381 gatgaatctg ttctttggtt cagtgttagc aacaatttcg cttaccgtac ctgtcgtcac
    22441 gctaattgcc tttatgacgg gtaacgaatt gcagtttgca cttggtgcgc cagaaatggt
    22501 ggtgatggtg gcctctttag tgctgtgcca tatctccttc tccaccggac gtactaacgt
    22561 gctcaatggc gcagcgcatc tggcactgtt tgccgcctat ttgatgacga tatttgcctg
    22621 acagttatat ctcctccgat gacggaaatc gggagagata agataaattc ctgcttattg
    22681 tatttcttca tactacctgg acagctcgtt aatcagaatt gcgagcctgt accacaacct
    22741 gcacgaaacc actgcaacag ctgttgaaaa gccggaatgt actctttatt gcaaacattt
    22801 cagggatgaa gtgaagatga attgagacgt agtatccaca ccaagccgga attatccggt
    22861 gaggcgcaat gttgcggggg ctttatccct ggtggcattg gttgctggaa agagaaaacc
    22921 cccgcacgtt gagaggtaaa acctgacaac aaaacggggg ctaatcttga aaccagacac
    22981 ttcaagaata gccgcaaaat gttgtcatta caacaaggcg gctatatgac gctcgcgcag
    23041 tttgccatga ctttctggca cgacctggca gcaccgatcc tggcggggat tattgccgca
    23101 gcgattgtca gctggtggcg taaccggaag taaggtgaaa acgggcgtca ggctgccgtg
    23161 atggcaatat gcgcccgaaa ccggccgcag gggtaacagc ctgcggcctt ttttgtatct
    23221 ggcacccatg aaaaaagtct taacgcagaa cgctaagact ttatttttca agcaaaaaag
    23281 attacttgct ggtatccagt tcttcgaaac ctttcaccag atcatcaatc gctttcatct
    23341 gcttgaggaa cggttccagt ttagccagcg gcagcgcgga tggaccatca catttcgcat
    23401 gttccggatc cggatgcgct tcaataaaca gccccgccag acctaccgcc ataccggctc
    23461 gtgccagctc agccacctga gcacgacgac caccggaagc tgcgccaaac ggatcgcggc
    23521 attgcagtgc gtgggtcacg tcgaaaatca ccggcgagtt accagacact ttcttcataa
    23581 tgctgaagcc cagcatatcg acaaccaggt tgtcatagcc gaagttagca ccacgatcgc
    23641 aaagaatcac tttttcgttg ccgccttctt tgaatttatc aacgatatta cccatctgtc
    23701 ccgggctgac aaactgtggt ttcttgacgt taattaccgc accggttttc gccatggctt
    23761 caaccaggtc agtctggcga gcaagaaacg ccggcaactg aatcacatcc acgacatcag
    23821 caacgggctg tgcctgactt ggttcgtgaa cgtcggtgat aattttcacg ccaaaagtct
    23881 gcttcaactc ctggaagatt ttcatccctt cttccaggcc cggtccacga taagagtgga
    23941 tggaggagcg gttggctttg tcaaaagagg ctttgaacac gtaagggata cccagtttct
    24001 gggtcacagt tacgtagtgc tcgcaaatgc gcatcgccag atcgcgagat tccagcacgt
    24061 tcataccgcc aaacagtacg aacggcaggt catttgctac gttgatgtcg ccaatgctaa
    24121 ccactttttg tttcatagga tcgccttaat cttgagtaaa atgtcgatta attaatgcag
    24181 cacaatatgt ttatgcgcga tgttatttat ttgcgcacgg atcatttcgc tgatcgggtc
    24241 ttccggacac tgttcaacga aataacttaa atcgttcaac gcaacgtgtt cgcaatccag
    24301 ttgcgcataa atcaacccgc gatcgcgaat ttcataggga tcttcagggt tgaattgtaa
    24361 taaagcttcg ctggtgcgta acgccagctc catctgattt tcttccatca acgaggcttt
    24421 gagtgtatcc agcaatttgc ggattacctc aatgttatca gcttcatcaa ggtcttcata
    24481 aaacagttcc gccgacgggc tgatatttcc ctttaaccat ccgtccagca tatgttcgct
    24541 taacgattca ccgttaaaag gattaatcag ccaaatttcg ccatccggac attcaatgcg
    24601 caatatcagc tacgtaggga aaatcaccgg cagcagcggc aaatcgagac gattcgcgac
    24661 ccataataaa accgcaccta atgataccgc actgccctgt cgattcttta acacctggtc
    24721 cagccataat gcatcggaaa gacgataaac gccgcgtgag gctttaaatc cccagtcgcc
    24781 gtaaaacagc gcgatcagtt tttccagctg ctcttctaaa ggcagaagct ggctgatttc
    24841 ttccttcgcc agactaacga gacgctccag ttcgtcgtaa acatcttgcg agggaaaatc
    24901 gcggcggatt gcttcgcaag ccaggatcat gccttcgcac aatggcgctt tattaaattc
    24961 gaaatcagct aacgatctca tgacttaccc cagtaacggt acttttgtgg tggcgagttt
    25021 aatgatgatg tacagcacca ccaacgccag cgggaaggca ataatgcgcg cctgctgact
    25081 gtgcatacga cgataatcga gtgcaataaa acccaaaacg atataaatga taactccaaa
    25141 cagcttttca gtcagccact gtgcctgccc ggaaaatggc aggatgtgcg ctttagccat
    25201 caacgcaatg ccgcttaaca ataacaccgt atcgacaacc ggcggcacaa tgcgcgtcca
    25261 gcgagcaaat gcctgtggat gcttctgata acgtagccag aaacgtaagg ttaatagccc
    25321 aacagaaagc gcgatactaa taagatgaac actaagcagt gtagaaaaac ttgtcattga
    25381 taatagcggc cgagcgttac gcgctcgtta tcaccatagt cacggcaggt ttcgacgtca
    25441 tgataccccg cgaggataaa tgcttgtcgc accgcttcgc cctgctgcca gccatgttcc
    25501 agaagcagaa agccgccgga taccagcgcg ttacgcgact gttcgatgat atgcacgatg
    25561 tctgccattc cactgtctgc cgcaaccagc gcagtgagcg gctcaaagcg gacatcgcct
    25621 tgttgaagat gtgggtcctg ctcgtcaata tacggcggat tgctgacaat catcgcaaac
    25681 tgctgcccgg ctagcgcgct aaaccagtcg ctttgcagaa tgtggatatt tttgatcgcc
    25741 agatgctggg cattacgttg tgccagggag acagcatcag gcatacgatc tacagcgata
    25801 atttcgcagt ccgggcgctc gctagccagc gcaagcgcaa tcgccccggt acccgtcccg
    25861 agatcgagaa tacggcaagg ttgttcaggc aaccgcgcca gtgcctgctc caccagacac
    25921 tccgtatccg ggcgcggaat taaggtcgct ggcgaaacaa ataacggcaa cgaccagaat
    25981 tctcgcaccc cggttaaatg agcaatgggt tcaccatcgc gacgacgtgt cagtagcgca
    26041 tcaagttgct gacattgttc gtcagtcagc tgcgtttcac caaaggcgag aataaaagta
    26101 cgccctctgc cggtaacatg ctccagcagg atttcagcat cacgccgcgg gctttcgctc
    26161 gcctgaagtt ggcttattgc ttcacgtaac cagtgttgat attccattat tcctgctcgg
    26221 acaacgccgc cagttggtcg gcctgatgtt cctggataat cggttcaatc agcatatcca
    26281 gcttaccttc catcacttca tccaggcggt agagcgtcag gttgatgcgg tgatcggtaa
    26341 cgcgcccctg cgggaaattg taagtacggt tacggtcgct gcgatcgcca ctccccagca
    26401 ggttacgacg ggtagacgct tcggcctgtt ggcgttttgc catttcagca gcgtggatgc
    26461 gagcaccgag cactgaaagc gccttcgctt tgtttttatg ttgtgaacgt tcgtcctgac
    26521 attcaacaac aatcccggtc ggcaagtgag taatacgaat tgccgaatcg gtggtgttaa
    26581 cgtgctgacc acccgcccct gacgagcgga aagtatcaat gcgtaaatct gctgggttga
    26641 tgtccggcag ttctgcgtca ggcagttctg gcattaccgc aacggtacaa gcagaagtat
    26701 gaatacgacc ctgcgattcc gtagcaggaa cacgttgcac gcgatgaccg ccggattcaa
    26761 atttcagacg accatacaca ccatcaccgc taattttggc gatgatctct ttataaccac
    26821 catgttcacc ctcgctggcg ctcatgattt ctacccgcca gcggcgggct tcggcgtaac
    26881 ggctgtacat acggaacaga tcgcctgcaa acagcgctgc ttcgtcgccg ccggttccgg
    26941 cacggacttc gaggaaggcg ttacgttcgt catcaggatc ttttggtagt aatagaacct
    27001 gtaattgctg ttccagttgc tcgctttttt ctttagcttc gcgcagttca tcctgcgcca
    27061 tctcacgcat ttcaggatcg tcgagcatca tctgtgcggt ttcgatatct tcctgaacct
    27121 gttgccagtc ggtaaaacag cgcgaaacat cacttaactg cgcatattcg cgtgataatg
    27181 cgcgaaaacg ttcctggtcg gcgatagttt gcgcgtcacc cagcaacgcc tgaacttctt
    27241 catggcgttc atgcagggct tccagtttgg caacgataga aggcttcata ggcgtaaatg
    27301 caccctgtaa aaaaagaaaa tgatgtactg ctactccagc ccgaggctgt cgcgcagaat
    27361 attcaggcgt tcgttatccc cgtcacgggc ggcctgttga agtgatttcg ttggcgcatg
    27421 gatcaagcgg ttagtcagtt tccatgccag atcctgcata atggcttgcg cgtcgccgcc
    27481 ctgctcaagg gccgctaacg ctttggcggt taactcatcg cgaacgtgct ctgcctggct
    27541 gcgatactcg cgaatggttt cgctggcgct ttgtgctcgc agccacgcca taaattcgct
    27601 ggtttcctga gcgacaatag tttccgcctc aaccgctgcg gctttacgct gcgccaggtt
    27661 gtgcgaaatg atgctttgca gatcgtccac actataaaga taagcattcg ccagtttgcc
    27721 aacttccggc tcaacatcgc gcggaacggc aatatccacc aacagcattg gttggttacg
    27781 acgacttttt aatgcgcgct ccaccatgcc tttcccgata atcggtaacg ggctggcggt
    27841 ggaactgatg atgatatcgg cttcgcgcag acgttcgtcg atctcactca gggcaatcac
    27901 ttccgcgcca acttcatctg ccagaatttg ggcacgttcg cgagtgcggt tggcgataat
    27961 catcttctgt actttatgtt cgcgcagatg acgcgctacc agctcgatgg tttcgcccgc
    28021 gcctaccagc aacactgtga ccgtagagag cgattcaaag atctgccgcg ccagcgtaca
    28081 agccgcaaaa gcgacagaca cagcactggc accgatatct gtttcagtgc gaacgcgttt
    28141 cgctacagag aaagatttct ggaacatgcg ttccagttcg ctggccttca tatgaccttt
    28201 ttgcgaatcg acaaacgctt ttttaacctg accgaggatc tgcggctccc caagaaccag
    28261 tgaatccagg ccgctggcaa cacgcattaa atggctaacc gcgtcgttat cctgatgcca
    28321 gtagaggctt ttacgcagat cttcttcatt aagattgtga taatcgcaaa gccagcggat
    28381 taacgcctct tgcaggttat cctgctcttc aacgctaaga taaagttccg tgcggttgca
    28441 cgtcgacagc accacgccgc cctgcaccat cggctgcgca agcaggctgt caagcgcctg
    28501 atcgagctta tccggcgaaa acgatacacg ttctcgcagc gatacaggtg ccgttttatg
    28561 gttgataccg agtgctaaaa gggtcatgtc tgcgggaaat aataccaacg ttgatagggt
    28621 tagtctgctt gcatcataca ggatgcgtaa gatcaataaa agagagcgcc cccttttgga
    28681 gtaattgccg aaagccgtta gattctggca attaagacaa cttgaacata gacgatagcg
    28741 gacggtaacg ctagcattaa gggttataac tgcaacgtat ctcaaggact tgtcatcact
    28801 atgcccctgc ccgattttcg tcttatccgc ctgctaccgc tggctgctct tgtgctcact
    28861 gcctgttccg ttaccacgcc caaaggtcct ggcaaaagcc cggattcgcc acaatggcgt
    28921 cagcatcagc aagacgtgcg caatcttaat cagtatcaga ctcgcggcgc gttcgcttat
    28981 atttctgacc aacaaaaagt gtacgcccgc tttttctggc agcaaaccgg ccaggatcgc
    29041 taccgtctgc tgctcactaa cccattgggc agcacggaac tggagctgaa tgctcaaccg
    29101 ggtaacgtgc agttagtcga caataaaggt cagcgttata ccgccgatga cgccgaagag
    29161 atgattggca aattgaccgg aatgccaatt ccgctcaaca gcttgcgcca gtggatttta
    29221 ggtttaccgg gtgatgcaac cgactacaaa ctggacgacc agtaccgcct gagcgaaatt
    29281 acctacagcc agaatggcaa aaactggaag gttgtttatg gtggttatga caccaaaacg
    29341 caacctgcga tgccagccaa tatggaactc accgacggtg gtcaacgcat caagttaaaa
    29401 atggataact ggatagtgaa ataatgcgga cacagtggcc ctctccggca aaacttaatc
    29461 tgtttttata cattaccggt cagcgtgcgg atggttacca cacgctgcaa acgctgtttc
    29521 agtttcttga ttacggcgac accatcagca ttgagcttcg tgacgatggg gatattcgtc
    29581 tgttaacgcc cgttgaaggc gtggaacatg aagataacct gatcgttcgc gcagcgcggt
    29641 tgttgatgaa aactgcggca gacagcgggc gtcttccgac gggaagcggt gcgaatatca
    29701 gcattgacaa gcgtttgccg atgggcggcg gtctcggcgg tggttcatcc aatgccgcga
    29761 cggtcctggt ggcattaaat catctctggc aatgcgggct aagcatggat gagctggcgg
    29821 aaatggggct gacgctgggc gcagatgttc ctgtctttgt tcgggggcat gccgcgtttg
    29881 ccgaaggcgt tggtgaaata ctaacgccgg tggatccgcc agagaagtgg tatctggtgg
    29941 cgcaccctgg tgtaagtatt ccgactccgg tgatttttaa agatcctgaa ctcccgcgca
    30001 atacgccaaa aaggtcaata gaaacgttgc taaaatgtga attcagcaat gattgcgagg
    30061 ttatcgcaag aaaacgtttt cgcgaggttg atgcggtgct ttcctggctg ttagaatacg
    30121 ccccgtcacg cctgactggg acaggggcct gtgtctttgc tgaatttgat acagagtctg
    30181 aagcccgcca ggtgctagag caagccccgg aatggctcaa tggctttgtg gcgaaaggcg
    30241 ttaatctttc cccattgcac agagccatgc tttaagccgg gcaagctgag tttcggtgac
    30301 aacgtcacct tgttccagac gttgcatcgc gctctttaat acaccgcctg gaaaggatca
    30361 tgcctggccc gcacagtttt cggcagattc tttccaccaa tggacgcatg cctgaggttc
    30421 ttctcgtgcc tgatatgaag ctttttgctg gtaacgccac cccggtacta gcacaacgta
    30481 ttgccaaccg cctgtacact tcactcggcg acgccgctgt aggtcgcttt agcgacggcg
    30541 aagtcagcgt acaaattaac gaaaatgtac gcggtggtga tattttcatc atccagtcca
    30601 cttgtgcccc tactaacgac aacctgatgg aattagtcgt tatggttgat gccctgcgtc
    30661 gtgcttccgc aggtcgtatc accgctgtta tcccctactt tggctatgcg cgccaggacc
    30721 gtcgcgtccg ttccgctcgt gtaccaatca ctgcgaaagt ggttgcagac ttcctctcca
    30781 gcgtcggtgt tgaccgtgtg ctgacagtgg atctgcacgc tgaacagatt cagggtttct
    30841 tcgacgttcc ggttgataac gtatttggta gcccgatcct gctggaagac atgctgcagc
    30901 tgaatctgga taacccaatt gtggtttctc cggacatcgg cggcgttgtg cgtgcccgcg
    30961 ctatcgctaa gctgctgaac gataccgata tggcaatcat cgacaaacgt cgtccgcgtg
    31021 cgaacgtttc ccaggtgatg catatcatcg gtgacgttgc aggtcgtgac tgcgtactgg
    31081 tcgatgatat gatcgacact ggcggtacgc tgtgtaaagc tgctgaagcg ctgaaagaac
    31141 gtggtgctaa acgtgtattt gcgtacgcga ctcacccgat cttctctggc aacgcggcga
    31201 acaacctgcg taactctgta attgatgaag tcgttgtctg cgataccatt ccgctgagcg
    31261 atgaaatcaa atcactgccg aacgtgcgta ctctgaccct gtcaggtatg ctggccgaag
    31321 cgattcgtcg tatcagcaac gaagaatcga tctctgccat gttcgaacac taatcgaacc
    31381 cggctcaaag acccgctgcg gcgggttttt ttgtctgtaa tatccatttg tatgacctat
    31441 gcctccttca cctgccattt agttgacaga tgatgcgctc acggatgaaa cattattgtg
    31501 aacaaaatat tttcctcaca tgtgatgcct ttccgcgctc tgatcgacgc ttgctggaaa
    31561 gaaaaatata ctgccgcacg gtttacccgt gacctgattg ccgggataac cgtcgggatt
    31621 attgctatcc cgctggcgat ggcgttggct attggtagtg gtgtggcccc ccagtacggt
    31681 ttatataccg cagctgttgc ggggattgtc attgctctaa cgggtgggtc acgctttagc
    31741 gtttccggtc cgactgcggc atttgtggta attctctatc ccgtttcgca acagtttgga
    31801 ctggcaggac tgctggttgc gaccttgttg tcgggaatct ttttgattct gatggggctg
    31861 gcacgctttg gtcgtctgat tgagtatatt ccggtttccg tcaccttagg tttcacctcg
    31921 ggtatcggga tcaccatcgg taccatgcag attaaagatt ttctcggtct gcaaatggcc
    31981 catgtcccgg aacattatct acaaaaagtc ggcgcattat ttatggcgct gccgaccatt
    32041 aatgtgggtg atgctgccat tggcattgtg acgcttggca ttctggtttt ctggccgcgt
    32101 ctgggcattc gtttacccgg tcaccttccg gccttgctgg ctggttgcgc ggtgatgggg
    32161 attgttaacc tgctcggcgg acatgttgct accatcggtt cgcaattcca ctacgtcctg
    32221 gccgatggtt ctcagggtaa cggtattccg caactgctgc cgcaactggt gctgccgtgg
    32281 gatctgccta attcagaatt cacgctaacc tgggattcta ttcgcaccct gctgcctgcg
    32341 gcattctcaa tggcaatgct cggggcgatc gaatctctgc tctgcgctgt ggtactggat
    32401 ggtatgaccg ggactaagca caaggcgaat agtgaactgg tcggtcaggg gctggggaat
    32461 atcatcgctc cattctttgg tggtattacc gctactgctg ccatcgcgcg ttctgccgct
    32521 aacgtccgtg ccggggcaac ttcccctatc tcggcggtga tccactctat tctggttatt
    32581 ctcgccctgc tggtactggc accactgctc tcctggctgc cgctttccgc catggcagcc
    32641 ctgctgttga tggtggcgtg gaacatgagt gaagcgcaca aagtggtcga cttgctgcgt
    32701 catgcgccga aagatgacat catcgtcatg ctgctgtgta tgtcactgac cgtgctgttt
    32761 gatatggtta ttgccatcag cgtggggatc gtgctggcat cgctgctgtt tatgcgtcgt
    32821 atcgcacgta tgactcgcct ggcaccggta gtcgtagatg ttccagacga tgtcctggtt
    32881 ctgcgcgtta ttggcccgct gttttttgct gctgctgaag gcttattcac ggacctggag
    32941 tcacgtcttg aaggcaaacg gattgtgatt ctgaagtggg atgccgttcc ggtacttgat
    33001 gctggtggtc ttgatgcgtt ccagcgtttt gtgaagcgtc tgcccgaagg atgtgaactg
    33061 cgcgtgtgca acgtggaatt ccagccactg cgaactatgg ctcgcgcagg cattcaaccg
    33121 atcccgggac gcctcgcgtt cttcccgaat cgtcgcgcgg cgatggcgga tttataagta
    33181 cgagattgac cagtcagcgc aaactgactg gtcagcaaac tgcattattt gttagctatg
    33241 acggcgatta tcgctacggc ggcaacgttt gtcatagtga cgaacccacc agtaacggtc
    33301 gcacacctgt tcatgcccgc caacacgggc acccgccagc cagagaatgg caccgacgaa
    33361 aatacttagc actgcaccat gtgcgaaata ctggggcata ttaaactgtg gtaactggtt
    33421 gaggattgaa taccccacgc cgaccaccat taccaccaga cccaacccca tgagcacgtt
    33481 accgagtatc gaagcgtttt tgcgtttcat atgtcacctc cggaactttc tgggttgtaa
    33541 cagggaatac ccctcttcct tatgtgtaaa gtatagacaa caccctgtgg gttatgtgcg
    33601 ggcgtaatca caattacaac ccttatttca acaaaacttt acaaataaac gcctgaacac
    33661 cttgactttc taatagtcca ctcaggcaat tattacgtta tctgcatttg atacgcagtt
    33721 ttttgtcaag cggggccgca accagtaaac tacgcgccag ttatgtacac actcaggaca
    33781 aaaaaacgtg acgattaaat tgattgtcgg cctggcgaac cccggtgctg aatacgccgc
    33841 aacgcgacat aatgctggtg cctggttcgt tgacttactg gcagagcgtt tgcgcgctcc
    33901 gctgcgcgaa gaggctaaat tctttggtta tacttcgcga gtcacccttg gaggcgaaga
    33961 tgtccgcctg ttagtcccga ctacatttat gaatctcagc ggcaaagccg ttgcggcgat
    34021 ggccagtttt ttccgcatta atccggacga aattctggtg gcccacgacg aactggatct
    34081 gcctcctggc gtcgccaaat ttaagttggg cggtggccat ggtggtcaca atggactgaa
    34141 agacatcatc agtaaattgg gtaataaccc taactttcac cgtttacgca tcggaatcgg
    34201 tcatccgggc gataaaaata aagttgtcgg ttttgtgtta ggcaaaccgc ctgttagtga
    34261 acagaagtta attgatgaag ccatcgacga agcggcgcgt tgtactgaaa tgtggtttac
    34321 agatggcttg accaaagcaa cgaaccgatt gcacgccttt aaagcgcaat aagtcgttgt
    34381 ctgcggcatt tttgccgagt gccgtgtata ataggcaaag ttatttccat ttctgcaatc
    34441 tgttagcaat aacaggttga ttattaagat attaaggtga tttaaatcat gggattcaaa
    34501 tgcggtatcg tcggtttgcc caacgtcggg aaatctaccc tgttcaacgc gctgaccaaa
    34561 gccggtattg aagcggccaa ctttccattc tgcaccattg agccgaacac aggcgtcgta
    34621 ccaatgcctg accctcgcct ggatcaactg gctgaaatcg taaaaccgca acgtacgctt
    34681 cccacgacca tggaatttgt cgatatcgcc ggtctggtaa aaggcgcatc gaaaggcgaa
    34741 ggtctgggta accagttcct gaccaacatc cgtgaaaccg aagcgatcgg tcacgttgtt
    34801 cgctgctttg aaaatgacaa catcattcac gtttccggca aagttaaccc ggctgacgat
    34861 attgaagtta tcaacaccga actggcgctg gcagacctcg acacctgcga acgtgcgatt
    34921 catcgcgtac agaagaaagc caaaggtggc gataaagacg cgaaagctga gctggcggtc
    34981 ctggaaaaat gcctgcccca gctggaaaac gcaggtatgc tgcgcgcgct ggatttaagc
    35041 gctgaagaga aagcggctat tcgttacctg agcttcctga cgctgaaacc aacaatgtac
    35101 atagccaacg tcaacgaaga tggttttgaa aacaacccat atcttgacca ggtgcgtgaa
    35161 atcgcggcga aagaaggttc tgttgtggtt ccggtttgtg ctgctgttga agcagacatt
    35221 gccgaactgg acgacgaaga acgtgacgag tttatgcagg agcttgggct ggaagaaccg
    35281 ggcctgaacc gtgtgatccg tgccggttat aagctgctga acctgcaaac ttacttcacc
    35341 gctggggtga aagaagtgcg tgcatggacc attccggttg gagcaaccgc gccgcaggca
    35401 gcgggcaaaa tccatactga ttttgaaaaa ggctttatcc gtgcacaaac catctcgttt
    35461 gaagatttca tcacttacaa aggtgaacaa ggcgcgaaag aagcaggcaa aatgcgtgca
    35521 gaaggtaaag attacatcgt gaaagatggc gatgtgatga acttcctttt caacgtctaa
    35581 ttaatttatt gtctcatgag gtttcgctaa atctcatgaa gagcgcaaaa gtatataaaa
    35641 tccacgcaat tgcgtggatt tttcatttca ccgagtccta atcattcttg taacatccta
    35701 cacttttgtg cttattgcct acagtgaacc acgagaataa ttaaagacat aagtgttcag
    35761 atgactgata tctcattagc agtgatataa cgtcagtttt tgttccacta cacatcagcc
    35821 ctcttcacag ctgatcaata ttgatctcgc tccaatatgc tggcgagaaa aggacttacc
    35881 tgaagggtaa tatcccgcag ataaagtccc cgaataacgt gattacgtgc gatcctggca
    35941 agatgaaaat gataaccagt agcacaataa taacgatagg ccagaaaatc tattgaacaa
    36001 aaatgaatat cgatgttttt tgctttttct tattagtaat gcaaaaatgg cgcatattgt
    36061 ttttgttatg cagatacatc aagcgaaaat cccataacta attaggatta ttacaaaaaa
    36121 tcttcaccaa attttttaac cgctgtttgt tgtaatattt ttgccatatt catagtaacg
    36181 aattggaata agtaacgaca acttagggaa taactcgata taaactcacc tttgtaaggg
    36241 gggggttaaa tatatattaa ttcctgtgtt cctgattttg cccggtaata gagaattatt
    36301 ctcagagaag ctcatcatga ttgcaccata cagatatcat gatgataaga ggatattatt
    36361 cattgactta aacaaaatga caaaatccat agacccatag cctgggtact atataggagt
    36421 ggtatgggca tcaaacaaca caatgggaat accaaagccg atcgtctcgc tgaattaaaa
    36481 atccgttcgc cctcaattca actgataaaa tttggcgcta ttggtttgaa tgcaattatc
    36541 ttttcccccc tgctgatagc tgctgataca ggaagtcaat atggcaccaa tattactatt
    36601 aatgatggtg acagaattac aggagatacc gccgatccat caggaaacct ctatggtgta
    36661 atgaccccag caggaaacac gcctggcaat atcaacctgg gtaatgatgt caccgtcaat
    36721 gtcaacgacg cctctggata tgcaaaagga atcattattc agggcaaaaa cagctccctg
    36781 acagctaacc gactcacagt agatgttgtt ggtcaaacct ctgccatcgg cattaactta
    36841 attggtgact atacccatgc tgacttaggc acaggcagca ccattaagag taacgatgac
    36901 ggcatcatta ttgggcatag ctcaacacta acagccactc aattcaccat tgaaaactcg
    36961 aacggtatag gcctaaccat caatgactat ggcaccagtg tcgatcttgg aagcggaagt
    37021 aaaatcaaga ccgatggaag tacaggtgtt tatatcggtg gtctcaacgg caataacgcc
    37081 aatggtgctg cgcgttttac ggcgacagac ctgacaatcg atgttcaggg ctacagcgcc
    37141 atggggataa acgtacagaa aaactctgtt gtcgatctcg gaacaaacag taccattaaa
    37201 accaatggcg ataatgctca cggcctctgg agctttggcc aggttagcgc gaatgcactc
    37261 actgttgatg taactggagc cgcggccaat ggcgtcgaag ttcgtggtgg tacaaccact
    37321 atcggtgcag atagccatat ttcttccgcg cagggcggtg gcctcgtcac cagtagttca
    37381 gacgcgacaa tcaatttttc tggcacggca gcgcaacgaa acagcatctt ttccggcggt
    37441 tcttatggtg cctcggccca gacggcaacg gctgttatca acatgcaaaa taccgatatt
    37501 acggttgatc gtaatggcag tctggcgctg ggtttgtggg cgctcagcgg cggtagaata
    37561 accggagaca gtttggctat caccggcgcg gcaggagcca gagggattta tgccatgacc
    37621 aacagccaga tcgacctcac gagcgatctg gtcattgata tgagtacacc cgaccagatg
    37681 gccatcgcaa cgcaacatga cgatggttat gccgccagcc gcatcaacgc ctcgggtcgt
    37741 atgcttatca acggtagcgt tctttccaaa ggtgggctaa tcaatctgga tatgcaccct
    37801 gggtcggttt ggacaggttc ctccctcagc gataatgtca atggcgggaa actggacgtt
    37861 gcaatgaata acagcgtctg gaacgtaaca agtaattcta atctcgacac gctggcgctg
    37921 agccattcaa ctgtcgattt tgccagccac gggtcaactg ccggcacatt taccacatta
    37981 aacgtagaga acctgagcgg taacagtacc tttattatgc gtgctgatgt tgttggcgag
    38041 ggtaatggcg ttaataatag aggggattta ttgaatatca gcgggagtag tgctggtaat
    38101 cacgtattgg ctatccgcaa ccagggcagc gaggccacaa cgggaaatga agttctgaca
    38161 gtggtaaaaa ccactgacgg cgcggcctcg ttcagcgcgt cttctcaggt tgagttgggg
    38221 ggatatctgt acgatgtgcg taaaaatggc actaactggg agctttacgc ttccgggaca
    38281 gttccggaac cgactcctaa tcctgaaccc acaccagctc ccgctcagcc tcccatagtc
    38341 aaccccgatc ctacgcctga acccgctccc acgcctaaac ccaccacgac cgcagatgct
    38401 ggcggcaatt atctcaatgt cggttactta ttgaactatg ttgaaaaccg tacgctgatg
    38461 caacggatgg gtgacttgcg aaatcagagt aaagacggta atatctggtt gcgcagttat
    38521 gggggaagcc tggactcctt tgccagtggc aaactgagcg gctttgacat gggttacagc
    38581 ggtatccagt ttggtgggga taaacgtctc tctgatgtaa tgccgttgta tgtcggtctg
    38641 tatattgact caacacatgc atcgccggac tatagcggag gcgacggtac cgcacgttca
    38701 gactacatgg gaatgtacgc cagttacatg gcacaaaacg gtttttacag cgatctcgtt
    38761 ataaaagcat cgcgccagaa aaatagtttc cacgtactgg acagtcagaa caacggcgtt
    38821 aacgccaacg gcactgcgaa tggaatgagc atctccctgg aagccgggca gaggttcaac
    38881 ctgtccccta ctggttatgg gttctatata gagccgcaaa cccagcttac atacagccac
    38941 cagaatgaga tggctatgaa ggcgagtaat ggcctcaata tacatctgaa tcactacgaa
    39001 tcgctgctgg ggcgtgccag catgatactg gggtatgaca tcaccgcagg caacagccag
    39061 ctgaatgtct atgtgaagac tggcgctatc cgcgagtttt caggggatac cgaatatctg
    39121 ttgaacgact cccgggagaa gtacagtttc aaaggtaatg gctggaataa cggcgtggga
    39181 gtcagtgcac agtataacaa acagcacaca ttctatctcg aagcggatta cacgcagggt
    39241 aacctctttg atcagaagca agtcaacgga ggatatcgct tcagctttta agtgcgggat
    39301 agacaagccc ttaaacgatg ctgaaaattg ttagcgtgcg ccggaatgaa atacagatca
    39361 ctctgatgta tcagaaatcg tttctgtctt tcatacccgg cgcttaaact gccctgcatc
    39421 aatgccatgt tgcttcattt tccgccacag cgtagtgcgg ccgatcccaa gtaaagccga
    39481 catttcctga atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct ctttttcaac
    39541 ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg acatcatctg tcgcctgctc
    39601 ggtaaacaga tgttcaggta aatcactgac gcgaatgcgc ccgttatcac tactcagagc
    39661 aagattctcg atgacgctgt aaagttcaaa atcgttgcct ggccatgcac aagaaactag
    39721 gcgagcgagg gcatcgtcat caattttcag ccgcgtagag aagcgttttt caagactgcg
    39781 taatttgtta ttcaccagcg ccggaatgct gccacgccgc atacgcagag gcgggatggt
    39841 aatttcaaat gcatgcagcg cgtaatacag ctggcgacta aaacgatttt gttccaccag
    39901 cattgcgagg tccgcggtcg ttgttgcaat cactttgaca tcaattggta ttaaacgccg
    39961 cgcatccagt cgcgtgataa ccccctgctt gataacctgc agcaaagcag actgtaactc
    40021 caccgccaga tattcaatct tttcaagaaa cagcgtgccg ccgtgtgcca gttccagccg
    40081 actcagacgg ccattttcat tgtccgtgcg atcgccacca ataaattctt ccgccagcgc
    40141 agcatcacca tataactcac aattgacggc gatataagga cctgcagcac gctcgctttc
    40201 attatgaatt gcctgactta gcagtgcctt gcccacgccc tcttctccac aaagcaggac
    40261 aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg tttgcggatc
    40321 gtcctgtggc atatgagcga aggtatggct gacttttcct aattgactgg tcattaactg
    40381 ccgcatctgt tccacaggat ggagcaacaa aataaagctg gttccctgcg tttctattat
    40441 cggtttaagg gttatcaccg catcaataaa ctggtgctgg ctttcaaagg ttgcttctac
    40501 gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat acggcgggta acgttaagag
    40561 ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc gcgccgcctg
    40621 ggcattaata aattgcaaat taccctgctc gtcccagcta atcacgccat catccatact
    40681 ttctaacagg gcattaagtt gatttaaatg acggttagtt tcagcgagca aactgtccgt
    40741 cagcagtaaa ttaccgacct cgcgggcgat tgccaacgtc aacggcaaat cagctgcggt
    40801 agtttgctca accggacacg ccagcgctat tgttcccgtc aatcggccct tgctgtcaaa
    40861 caacggcgtt gcacaaaagg cccagttcca gagtgcctgt ttgaaatgtt gatcggccat
    40921 cgttttcacg gcctgaccag agatagccgc taacgatagc gcacaagtac caataattcc
    40981 ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt agctgctgca aggtttgcgg
    41041 atcgccatta cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg cacactctcg
    41101 cggtgccata tattcccagg catcttccag cgcagcctgc ccgagcgtca gcatcgcttt
    41161 cttacgccga taaatagaag caaatgtcac gccctgggcc tgatgtggta cgttccatgt
    41221 ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt gcaattaagg gagatatgcc
    41281 ccgaccatcg ttgttaaaag cgccactcat atcccgcatc ccttatgaca aaaggtgttc
    41341 cggctcgcaa ttttaagtat taatacacgt aaaagtttca atatggaaca ttcattaaag
    41401 catatgtttc actatgaaac ataatttggg ctaattttct tttcccttgc ctgatgcaca
    41461 atggattcat cggcagtcag tggtcgccgt gtcgttgaac atcatccatg ccctaccgta
    41521 attgctggag caaaataatg aaaaaattga tcaatgatgt gcaagacgta ctggacgaac
    41581 aactggcagg actggcgaaa gcgcatccat cgctgacact gcatcaggat ccggtgtatg
    41641 tcacccgagc tgatgcccct gttgcaggaa aagtcgccct gctgtcgggt ggcggcagcg
    41701 gacacgagcc gatgcactgt ggttatatcg gtcaggggat gctttcgggg gcctgtccgg
    41761 gcgaaatttt cacctcaccg acgcccgata aaatctttga atgctccatg caaattgatg
    41821 gcggcgaagg tgtactgttg attatcaaaa attacaccgg cgatattctt aactttgaaa
    41881 cagcgaccga gttactgcac gatagcggcg taaaagtgac cactgtggtc attgatgacg
    41941 acgttgcggt aaaagacagt ctttataccg ccgggcgacg cggcgttgcc aacaccgtat
    42001 taattgaaaa actcgtaggc gcagcggcgg agcgtggcga ctcactggac gcctgtgcgg
    42061 aactggggcg taagttgaat aatcaaggcc actcaatagg tatcgctctc ggtgcctgta
    42121 ccgttcctgc cgcgggcaaa ccttctttta cccttgcgga taatgagatg gagtttggcg
    42181 tcggcattca tggtgagcca ggtattgacc gccgcccttt ctcttccctt gatcaaaccg
    42241 tcgatgaaat gttcgacacc ctgctggaac atggctcata ccatcgcact ttgcgtttct
    42301 gggattatca acaaggcagc tggcaggaag aaccacaaac caaacaaccg ctccagtctg
    42361 gcgatcgggt gattgcactg gttaacaatc ttggcgcaac tccgctttct gagctgtacg
    42421 gcgtctataa ccgcctgacc acacgttgcc agcaagcggg attgactatc gaacgtaatt
    42481 taattggcgc gtactgcacc tcactggata tgaccggttt ctcaatcacc ttactgaaag
    42541 ttgatgacga aacgctggct ctctgggacg ccccggtcca caccccggcc cttaactggg
    42601 gtaaataagg agaaagcaat gtcactgagc agaactcaaa ttgttaactg gctcactcgt
    42661 tgtggcgata ttttcaacac cgagagcgag tatcttaccg ggctggatcg cgagattggc
    42721 gacgctgacc acgggctaaa tatgaaccga ggctttagca aagtggtgga aaaactccct
    42781 gctatcgcag ataaagatat cggtttcatt ctcaaaaata ccggtatgac gctgctttcc
    42841 agcgtcggtg gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc cgcacaggcg
    42901 acccaggcac ggcaaagcct gacactggaa gagctttatc agatgttccg cgatggcgcg
    42961 gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg tgatgtgtgg
    43021 gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt tccggtggcg
    43081 ctcgaagctg ccagtagcat cgccgaatcc gctgcacaaa gtacgattac gatgcaagcc
    43141 cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg gtcaccagga tcccggcgcg
    43201 acctcggtga tgtttatgat gcaaatgttg gcgttagccg caaaagagta aggaattggt
    43261 gatggtaaac ctggtcatag tttcacatag cagccgactg ggagaaggtg tcggtgaatt
    43321 agcccgtcag atgttaatga gtgatagttg taaaatcgcc attgccgcgg gaattgacga
    43381 tccacaaaat cccattggta ccgatgccgt caaagtgatg gaggccatcg aatctgttgc
    43441 tgatgccgac catgtgctgg tcatgatgga tatgggtagc gcattattga gtgctgaaac
    43501 tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta cgtttgtgtg ctgcgccgtt
    43561 ggtcgaaggt acactggcag caacggtcag cgcggcctcg ggggcggata tcgacaaagt
    43621 tatctttgac gctatgcatg cgctggaagc caaacgtgaa caactgggtt taccgtcctc
    43681 cgacactgaa atctctgaca catgtcctcc gtacgatgaa gaagcccgtt ctctggcggt
    43741 ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc tcccggctgg tttatacctt
    43801 atcgacattt aatgccgata tgttgctgga aaaaaacggc aaatgcgtca caccagagag
    43861 tattaaccag attgcgttac tacaagttcg ctataacgat acgctgcgcc tgattgcgaa
    43921 agggccagaa gctgaagagg cactgatcgc tttccgtcac ctggctgaag ataactttgg
    43981 tgaaacggag gaagtcgctc cacctactct gcgtcccgtt ctgcctgttt cgggtaaagc
    44041 cttttattat caaccagttt tatgtacggt acaggcaaaa tcaaccctga ccgcggaaga
    44101 agaacaagat cgattacgcc aggctattga cttcacgtta ttagatctga tgacgttaac
    44161 agcgaaagca gaagccagcg ggcttgacga tattgccgca atcttttctg gtcaccatac
    44221 actgttaggt gatccggaac tgctggcggc ggcaagcgaa ctccttcagc atgaacattg
    44281 cacggcagaa tatgcctggc agcaagttct taaagaactt agccagcaat accagcaact
    44341 ggatgatgaa tatctacaag ctcgctatat tgatgtggac gatcttctgc atcgcaccct
    44401 ggtccacctg acccaaacga aagaagaact cccgcagttt aactcgccaa ctattctact
    44461 ggcggagaac atttatcctt ccacagtact gcaactggat ccggcggttg taaaaggtat
    44521 ctgccttagc gccggaagtc cggtatccca cagcgcccta atcgcccgtg aactggggat
    44581 tggctggatt tgccagcagg gtgagaaact gtatgcgata caaccagaag aaacgctaac
    44641 gctggacgtt aaaacgcaac gtttcaaccg tcagggttaa gccaccgttc ggatggcatc
    44701 gttctgatgt catccggcaa tttacccagc actgatgttt tttaactacc tgttatattt
    44761 tttaccaatt tatgatgatt tttcttcgct aatcgcgaac caaatgtcgc tggctggcta
    44821 ataaacagac gttatttgat cactctattt cgccgttatt tatcccttta attccctttt
    44881 ctaaaatgcc tgaccgttcg cagaattaga tttcgatcat gcagctagtg cgatcctgaa
    44941 ctaaggtttt ctgatacttg aataccgttt tattcggttt cgccaaagga gaatgattga
    45001 tgaaatcccc cgcaccttct cgcccgcaaa aaatggcgtt aattccagcc tgtatctttt
    45061 tgtgtttcgc tgcgctatcg gtgcaggcag aagaaacacc ggtaacacca cagccgcctg
    45121 atattttatt agggccgctg tttaatgatg tgcaaaacgc caaacttttt ccggaccaaa
    45181 aaacctttgc cgatgccgtg ccgaacagcg atccgctgat gatccttgct gattatcgga
    45241 tgcagcaaaa ccagagcgga tttgatctgc gccatttcgt taacgtcaat ttcaccctgc
    45301 cgaaagaagg cgagaaatat gttccgccag aggggcagtc actgcgcgaa catattgacg
    45361 gactttggcc ggtattaacg cgttctaccg aaaacaccga aaaatgggat tctctgttac
    45421 cgctgccgga accttatgtc gtgccgggcg gacgttttcg cgaggtatat tactgggaca
    45481 gttacttcac catgttagga cttgccgaaa gtggtcactg ggataaagtc gcggatatgg
    45541 tggccaattt tgctcatgaa ataaacactt acggtcatat tcccaacggc aaccgcagtt
    45601 actatttaag ccgctcgcaa ccgcccttct ttgccctgat ggtagagtta ctggcgcagc
    45661 atgaaggcga tgccgcgttg aagcaatacc tgccgcaaat gcaaaaagaa tatgcttact
    45721 ggatggacgg tgttgaaaac ctgcaagccg gacaacagga aaaacgcgtt gtaaaacttc
    45781 aggatggtac ccttctcaac cgctactggg acgatcgcga tacgccacga ccagagtcat
    45841 gggtggaaga tattgccacc gccaaaagca atccgaatcg acctgccact gaaatttacc
    45901 gcgacctgcg ctctgccgct gcgtctggct gggatttcag ctcgcgctgg atggacaacc
    45961 cgcagcagtt aaatacctta cgcaccacca gcatcgtacc ggtcgatctg aacagcctga
    46021 tgtttaaaat ggaaaaaatc ctcgcccgcg ccagcaaagc tgcaggagat aacgcgatgg
    46081 caaaccagta cgaaacgctg gcgaatgccc gtcaaaaagg gatcgaaaaa tacctgtgga
    46141 acgatcaaca aggctggtat gccgattacg acctgaaaag tcataaagtg cgcaatcagt
    46201 taaccgcggc cgccctgttc ccgctgtacg tcaatgcggc agcgaaagat cgcgccaaca
    46261 aaatggcgac ggcgacgaaa acacatctgc tgcaacccgg cggcctgaac accacgtcgg
    46321 tgaaaagtgg gcaacaatgg gatgcgccaa atggctgggc accgttacag tgggtcgcga
    46381 cagaaggatt acaaaactac gggcaaaaag aggtggcgat ggacattagc tggcacttcc
    46441 tgaccaatgt tcagcacacc tatgaccggg agaaaaagct ggtggaaaaa tatgatgtca
    46501 gcaccaccgg aacggggggc ggcggtggcg aatatccatt acaggatggc tttggctgga
    46561 ccaatggcgt gacgctgaaa atgctggatt tgatctgccc gaaagagcaa ccgtgtgaca
    46621 atgttccggc gacgcatccg accgttaagt cagcaacgac gcaaccctca accaaagagg
    46681 cacaacccac accttaacca gcgcttactc cgacaacgtc attcggcttc tccggatggc
    46741 gttgtcggca gataacacca tatctatggt tttttctaca cagacggttt ttcaagtcct
    46801 ggcgggaagt attgcgcaaa tgtttgccat acgtggttgc cgaagatagc gtcatttcta
    46861 cgggtaagtg tttcactttc atcacccggc gatttacgcc gacgttggct atataaagca
    46921 tcaattgcta ccgaatattg ttacggtgtt cactcatcaa cgtttttttg ctttctggag
    46981 ttaaattaat aatcacataa tccagacact ccagagaatt tagcaaccaa aaaaagaatt
    47041 cagaccattc ctcttcgcgt aactgaagca ttgccgaacg ggtattttgg cttaatctgc
    47101 cagaaggatt gatcaattct aaatgttcaa taag
//
LOCUS       DAJVPP010000019        46593 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVPP010000019 DAJVPP010000000
VERSION     DAJVPP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.019"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(120..195)
                     /locus_tag="OQM34_001068"
     tRNA            complement(120..195)
                     /locus_tag="OQM34_001068"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Lys,seq:ttt)
     gene            complement(198..273)
                     /locus_tag="OQM34_001069"
     tRNA            complement(198..273)
                     /locus_tag="OQM34_001069"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(238..240),aa:Val,seq:tac)
     gene            complement(410..485)
                     /locus_tag="OQM34_001070"
     tRNA            complement(410..485)
                     /locus_tag="OQM34_001070"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(450..452),aa:Lys,seq:ttt)
     gene            complement(650..1441)
                     /gene="cpoB"
                     /locus_tag="OQM34_001071"
     CDS             complement(650..1441)
                     /gene="cpoB"
                     /locus_tag="OQM34_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1451605.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1451..1972)
                     /gene="pal"
                     /locus_tag="OQM34_001072"
     CDS             complement(1451..1972)
                     /gene="pal"
                     /locus_tag="OQM34_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1451606.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMFSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2007..3299)
                     /gene="tolB"
                     /locus_tag="OQM34_001073"
     CDS             complement(2007..3299)
                     /gene="tolB"
                     /locus_tag="OQM34_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1451607.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3432..4640)
                     /gene="tolA"
                     /locus_tag="OQM34_001074"
     CDS             complement(3432..4640)
                     /gene="tolA"
                     /locus_tag="OQM34_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1451608.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEAD
                     DIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFY
                     DASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEV
                     FKNAPLDFKP"
     gene            complement(4705..5133)
                     /gene="tolR"
                     /locus_tag="OQM34_001075"
     CDS             complement(4705..5133)
                     /gene="tolR"
                     /locus_tag="OQM34_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1451609.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5137..5829)
                     /gene="tolQ"
                     /locus_tag="OQM34_001076"
     CDS             complement(5137..5829)
                     /gene="tolQ"
                     /locus_tag="OQM34_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1451610.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5826..6230)
                     /gene="ybgC"
                     /locus_tag="OQM34_001077"
     CDS             complement(5826..6230)
                     /gene="ybgC"
                     /locus_tag="OQM34_001077"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1451611.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6380..6673)
                     /gene="ybgE"
                     /locus_tag="OQM34_001078"
     CDS             complement(6380..6673)
                     /gene="ybgE"
                     /locus_tag="OQM34_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1451612.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6673..6786)
                     /gene="cydX"
                     /locus_tag="OQM34_001079"
     CDS             complement(6673..6786)
                     /gene="cydX"
                     /locus_tag="OQM34_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1451613.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6801..7940)
                     /gene="cydB"
                     /locus_tag="OQM34_001080"
     CDS             complement(6801..7940)
                     /gene="cydB"
                     /locus_tag="OQM34_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1451614.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7956..9524)
                     /gene="cydA"
                     /locus_tag="OQM34_001081"
     CDS             complement(7956..9524)
                     /gene="cydA"
                     /locus_tag="OQM34_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1451615.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10373..13006)
                     /gene="mngB"
                     /locus_tag="OQM34_001082"
     CDS             complement(10373..13006)
                     /gene="mngB"
                     /locus_tag="OQM34_001082"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCS1451616.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKILWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(13024..14940)
                     /gene="mngA"
                     /locus_tag="OQM34_001083"
     CDS             complement(13024..14940)
                     /gene="mngA"
                     /locus_tag="OQM34_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1451617.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15110..15832
                     /gene="mngR"
                     /locus_tag="OQM34_001084"
     CDS             15110..15832
                     /gene="mngR"
                     /locus_tag="OQM34_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1451618.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15935..16804)
                     /gene="sucD"
                     /locus_tag="OQM34_001085"
     CDS             complement(15935..16804)
                     /gene="sucD"
                     /locus_tag="OQM34_001085"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1451619.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16804..17970)
                     /gene="sucC"
                     /locus_tag="OQM34_001086"
     CDS             complement(16804..17970)
                     /gene="sucC"
                     /locus_tag="OQM34_001086"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1451620.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIITAKGLTDAAQQVVAAVEGK"
     gene            complement(18064..19281)
                     /gene="odhB"
                     /locus_tag="OQM34_001087"
     CDS             complement(18064..19281)
                     /gene="odhB"
                     /locus_tag="OQM34_001087"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1451621.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19296..22097)
                     /gene="sucA"
                     /locus_tag="OQM34_001088"
     CDS             complement(19296..22097)
                     /gene="sucA"
                     /locus_tag="OQM34_001088"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1451622.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22398..23114)
                     /gene="sdhB"
                     /locus_tag="OQM34_001089"
     CDS             complement(22398..23114)
                     /gene="sdhB"
                     /locus_tag="OQM34_001089"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1451623.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23130..24896)
                     /gene="sdhA"
                     /locus_tag="OQM34_001090"
     CDS             complement(23130..24896)
                     /gene="sdhA"
                     /locus_tag="OQM34_001090"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1451624.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24896..25243)
                     /gene="sdhD"
                     /locus_tag="OQM34_001091"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24896..25243)
                     /gene="sdhD"
                     /locus_tag="OQM34_001091"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1451625.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25237..25641)
                     /gene="sdhC"
                     /locus_tag="OQM34_001092"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25237..25641)
                     /gene="sdhC"
                     /locus_tag="OQM34_001092"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1451626.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26022..26129
                     /locus_tag="OQM34_001093"
     CDS             26022..26129
                     /locus_tag="OQM34_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451627.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26335..27618
                     /gene="gltA"
                     /locus_tag="OQM34_001094"
     CDS             26335..27618
                     /gene="gltA"
                     /locus_tag="OQM34_001094"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1451628.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28009..28575
                     /locus_tag="OQM34_001095"
     CDS             28009..28575
                     /locus_tag="OQM34_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1451629.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28626..31085
                     /locus_tag="OQM34_001096"
     CDS             28626..31085
                     /locus_tag="OQM34_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1451630.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            31100..31831
                     /locus_tag="OQM34_001097"
     CDS             31100..31831
                     /locus_tag="OQM34_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1451631.1"
                     /translation="MTFMKGLPLLLLVANLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31828..32875
                     /locus_tag="OQM34_001098"
                     /pseudo
     CDS             31828..32875
                     /locus_tag="OQM34_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            33027..34073
                     /locus_tag="OQM34_001099"
     CDS             33027..34073
                     /locus_tag="OQM34_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1451632.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34070..34861)
                     /gene="nei"
                     /locus_tag="OQM34_001100"
     CDS             complement(34070..34861)
                     /gene="nei"
                     /locus_tag="OQM34_001100"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1451633.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34897..35631)
                     /gene="pxpA"
                     /locus_tag="OQM34_001101"
     CDS             complement(34897..35631)
                     /gene="pxpA"
                     /locus_tag="OQM34_001101"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1451634.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35621..36553)
                     /gene="pxpC"
                     /locus_tag="OQM34_001102"
                     /pseudo
     CDS             complement(35621..36553)
                     /gene="pxpC"
                     /locus_tag="OQM34_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36547..37203)
                     /gene="pxpB"
                     /locus_tag="OQM34_001103"
     CDS             complement(36547..37203)
                     /gene="pxpB"
                     /locus_tag="OQM34_001103"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1451635.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37226..37969)
                     /gene="ybgI"
                     /locus_tag="OQM34_001104"
     CDS             complement(37226..37969)
                     /gene="ybgI"
                     /locus_tag="OQM34_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1451636.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38240..39721
                     /gene="dtpD"
                     /locus_tag="OQM34_001105"
     CDS             38240..39721
                     /gene="dtpD"
                     /locus_tag="OQM34_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1451637.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39871..41289)
                     /gene="phrB"
                     /locus_tag="OQM34_001106"
     CDS             complement(39871..41289)
                     /gene="phrB"
                     /locus_tag="OQM34_001106"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1451638.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41286..41795)
                     /locus_tag="OQM34_001107"
     CDS             complement(41286..41795)
                     /locus_tag="OQM34_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1451639.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41924..42211
                     /locus_tag="OQM34_001108"
     CDS             41924..42211
                     /locus_tag="OQM34_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCS1451640.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42266..42646)
                     /locus_tag="OQM34_001109"
     CDS             complement(42266..42646)
                     /locus_tag="OQM34_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451641.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42649..45459)
                     /locus_tag="OQM34_001110"
     CDS             complement(42649..45459)
                     /locus_tag="OQM34_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1451642.1"
                     /translation="MTLHRQPGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAW
                     DLAQRRLCVTDGLGRTRYHQWDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGG
                     KWRYVYDLQGHITETHDPLGRVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAV
                     TDPLQQNTRYQYDHHGQVVQITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERT
                     RLIRMTDAQSHSTRYGYDDSGHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQ
                     RDGQGRVRSRTDAMGRRTAFGYDAYGRLVTLTNENGERYRFRHDVLDRLAEQINPDGC
                     RQSYRYNALNAVTEVVFTGDRGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLL
                     EIRRQRHETDAPEVIRFSYDRLGRMLSEETAQGILTHQYDKLGNRTATTFPDGRTQRH
                     LYYGSGHLQQINLDREVISEFTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGM
                     RGVVPETFTDRQYSYSGEDELLKTRHSRRGETDYFYDPTGRITACRSEDEGYLASWQY
                     DAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYD
                     AEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEG
                     TGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQ
                     VWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGL
                     NLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGH
                     NYKNMTGESGPSTKNLMEYLTGNKTKAGSYHWDDIKDPSVTKPSYRVSGHGPGNPDGD
                     LPHLQVHQHGGSVRHIFFPWET"
BASE COUNT        11078 a        12777 c        11333 g        11405 t
ORIGIN      
        1 gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct
       61 aatcacccga tactgcgctg gatactacaa actacttcca ccgaacccac cgtaattagt
      121 ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa
      181 ctgagctaac gacccggtgg tgggtgatga cgggatcgaa ccgccgaccc cctccttgta
      241 agggaggtgc tctcccagct gagctaatca cccgacacta cgctggatac tacaactaac
      301 tatgttagtg gtgggtcgtg caggatgact cgacttcgtc tcgcccttcg gcgccgttgc
      361 tgatgcaacg ttatccttca cgttttacta ccgcttccac cttagcgatt ggtgggtcgt
      421 gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa ctgagctaac
      481 gacccacttt ttcgttgctt tcggtttgtt tgatatcccg tggcaacggc ggcatatatt
      541 actgatttca gacttgagcg caacaaaaat ttcgatgcag atcactcaac tgcttatgat
      601 tcgcacgaca cgaccagaaa taatgcgact tctggtcgtg tgttatgcat tacatcgcgt
      661 tcagacgttt ttgtgcctgt ttagcgccat cagtaccagg gtatttactg ataacctgct
      721 ggtatacggc tttcgctttt gcggtgtcac ctttgtcctg catgatgacg ccgactttaa
      781 acatcgcatc agcagccttt ggtgacttcg gatagttttt cactaccgaa gcaaaatagt
      841 acgccgcatc atctttttta cccttgttgt agtttaactg acccagccaa taattggcgt
      901 ttggcaggta agttgaatca gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt
      961 catcctggcg ggatttatcc tgcaccagcg caatagctgc attgtaatcc gtgtttgcat
     1021 cgccgctttt caccggcgcg ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg
     1081 atgccgtcgc accgctttga tcgctgctgg ttgattgcgc cgctgcacca ccactgctga
     1141 ggctgtcgat ctgcaacagg atctgctttt gccgctccac gacctgattc agttgatact
     1201 gattttcctg aatctgacca cgcagggaat caatatcgga ttgattatca gaaagttgtt
     1261 gctggagttg ggttaaaagc tggctgtgag cattagaaat acgctcaagt tgagtgacgc
     1321 ggtcttcgac cgagcctgag ccgacactac tgattggtgc ctgagcaaaa gcggcccagg
     1381 gggccgctat accaaccagt aacgacagac tcaatagttg atgtctgaag ttactgctca
     1441 tgcaattctc ttagtaaacc agtaccgcac gacggttttt ggagtatgcc gcttcgtcat
     1501 gacccagtac tgcaggtttt tctttaccgt aagaaacgat ggagatctgg tctgcagaaa
     1561 cgcctttacc ctgcaggtac atcttaacgg cgttcgcacg acgttcaccc agggagatgt
     1621 tgtattccgg agtaccacgt tcgtccgcgt gaccttctac ggtgactttg taagacgggt
     1681 tgctacgcag gaagtttgca tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt
     1741 acttgtccag atcgaagtaa acgatgttgt tctgctgcag ctgttgcatt tgcagacgag
     1801 cctgctcttc ggaaaacatg ttgccgttgc cgccgttcgc atccatacca gtgccggcac
     1861 ccagcatgcc ttcgctgccg tcattgctgg cgttcttgtt ggaagaacat gccgcaattg
     1921 ccataacagg cagagcaatc atcagccctt tcagcacttt gttcagttgc atttcaatga
     1981 ttcctttact attcaattaa ttattatcac agatacggcg accaggcagg gaatttgacc
     2041 tgtccatcag ttgccggaag acgcgctttg aaacgcccat ctgtagaaac caaattcagc
     2101 acggatccca tcccctgaga agagctgtag attaccatag tgccgttagg tgccagactt
     2161 ggcgtttcat ccaggaacgt ggacgacaga acttgtacgc ctcccgttgc cagatcttgt
     2221 ttggcaatgt gctgctgccc accattggag ctgaccatta ccataaattt accgtcgctg
     2281 ctgacatccg catcttggtt ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg
     2341 ttgatattca ctttataaac ctgcggacga ccggcctggt cagaagtaaa tgccaggttc
     2401 tggctatccg ggaaccaggt cggttcggta ttgttactgc gaccatcagt cacctggcgg
     2461 atctgaccag aagccaaatc cattacgtac aggttcagac taccggtttt cgacaaggcg
     2521 aatgccagtt tgctgccgtc tggcgagaat gcaggtgcac cgttgtgacg cgggaatgaa
     2581 gccacctgac gtacagcgcc atttgccagc gtctgaataa ccagcgcgga acgaccgctt
     2641 tcgaaggtca cataagccag tttagaaccg tctggtgacc acgccggaga catcagcggc
     2701 tgcggtgaac ggtgaacgac aaactggttg taaccgtcat agtcagatac gcgcagttca
     2761 tacgggaact gaccgccgtt ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct
     2821 ttaatgccgg tcagtttttc aaacacttca tcactggcgg tatgaccagc ataacgcagc
     2881 cactgcttgt tcactttgta cgagttctga gcaagtacag tacccggtgc gccgccagtg
     2941 tcaacaagtt gataagcaac attgtaagaa ccatccggat tcggagtgac ctgaccgaca
     3001 actacagcgt caatgcccag tgcggaccat gcagctggtt gtacttcctg cgcactaccc
     3061 ggctgctgtg gcagacgagc gcgatctaac ggattaaatt taccgctgtt acgcaagtca
     3121 gcagcaacga tgccgccaat atcttcaggt gccgcaccag gccccgccca ctggaaagga
     3181 acaacaccaa taggacgacc ggaatctaca ccgctgtcga tcacaatgcg gacttcagca
     3241 tgcagaactg atgcccacag tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc
     3301 atatctccct tatctggacc gatggcccac gataatttag cagaatgtta acaaactaaa
     3361 atacacaaaa ctaccagaac cccgtggcaa ccggtgcctg atgttgaccg tccgaacagt
     3421 caacatcgcg attacggttt gaagtccaat ggcgcgtttt tgaacacttc atatactgcc
     3481 tggcttggtg gtttcgggat cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt
     3541 gcgggatcgc caccttcagg tttgatatcc agtaacatac catcgggtgc cagttttatg
     3601 cgcagcgtac aggttttgcc tgcataggac gatgcgtcat agaacttact ttcgatagca
     3661 gatttaatct gcccggcata gttattgata tcggcccctg atgcgccatt gtttttagta
     3721 ttaccactcc cggcaggcga agcattgttc cctttcgccc ctccccccgt tttcggtgca
     3781 ttcttaccag agcttagctc accgaaaata tcatctgcct ctgcggcagc ttttgctgca
     3841 gcggcttttt cagctgctgc ttttgccgct gccgcttttt tgtctgcggc ggctttttct
     3901 gccgctgctt tcttatcagc tgctgcctta tcagcagccg ctttttctgc tgcttttttg
     3961 tcggctgcag ccttttcagc agccgctttc ttctcagctt ctgccttggc tttttcagca
     4021 gcttcagttg ccgctttctt tcttgcttca gctgcagctg cttctgccgc ttccgctttc
     4081 ttcttcagtg ccgcagcggc tacctcggct tttttctgcg cttcggctgc ggctttggcg
     4141 gcttctgctt ctgctttttt cttcgcgtct gcagccgctt tcttcgctgc ttcttccgca
     4201 gctttagcat ctgccgccgc tttcgctgcc gccacttcag cttgcttctg ctttaactcg
     4261 gcctgttttg cggcttcttc agcctgcttt ttctgctcct gagccgctaa ccgctctttc
     4321 tcaagttgct tcaggcgttc ctgttcagcc gcttgtttct cacgcagttc ttcagcagcc
     4381 tgctgttcct tcatcttgcg ctgttcatca gaacgcttcg cgcttgattc ctggctttgc
     4441 atgcgtttgt actgctcaac taccgcacct gaatcaacca tgacagcgtc gatggacgaa
     4501 ccaccgccgc ctccggctga agcttctata ttctcatcga acgaactcca gatcagcgcc
     4561 gcaaataaga tgacatgcag cactgctgaa ataattatcg cccgcttgag cttgtcgttt
     4621 tgttcggttg cctttgacac tctcggtttc caaaaactgt tcgcctgtta cccgctctct
     4681 ttcaagcaag ggaaacgcag atgtttagat aggctgcgtc attaaaccaa ccgatttcac
     4741 acccgcacta tgtaacaagt tcagtgcttt aattatttca tcgtaaggca catcttttgc
     4801 gccaccgatc agaaagaccg ttttcgggtt ggccttgaaa cggctggaca cttccgccac
     4861 cacctgctct ggtggtaaac gctccaggcg atctttctca accaccacgg tgtactgacc
     4921 aataccagac acttcaacaa tcactggcgg attatcgtta ctgctcaccg cctgtgattc
     4981 agtagcgtct ggcagatcga cctccacgct ctgggtgatg atgggcgctg tcgccataaa
     5041 gatcagcaac agcaccagca gtacgtccag caacggaaca atgttgattt cggacttgag
     5101 atcgcgacga cctcgtccac gcgctctggc catggcttac cccttgttgc tctcgctaac
     5161 ggtaaacgcc tggcggtgca gaatcgcggt aaactcttcc ataaagttgt cgtaattcag
     5221 ttccagtttg tttacgcgct ggttgagacg gttataagcc attaccgccg gaattgcggc
     5281 gaacagacca attgccgtcg caatcaacgc ttctgcgata ccgggcgcaa ccatttgcag
     5341 tgttgcttgt tttaccgccc cgagggcgat aaaggcgtgc atgatccccc agaccgtacc
     5401 aaacagacca atatacgggc tgatggagcc aaccgtaccg aggaacggaa tgtgcgtttc
     5461 cagattttca agttcacggt tcatggagat acgcatagca cgcgacgccc cttccactac
     5521 ggcttccggc gcatggctat tggcacgatg caggcgcaca aactctttga acccgctgta
     5581 aaagatttgt tccgaaccag tcagattatc ccgtttcccc tgactctctt gatagaggcg
     5641 agagagttcg attccagacc agaatttatc ttcaaacgct tcggcttcac gcgccgctgc
     5701 gttaagaata cgggttcgct ggataataat ggcccaggat gcgattgaaa aaccaatcaa
     5761 aatcaacatg ataagtttaa ccagaaggct agccttcagg aacaaatcaa ggatattcat
     5821 gtcagtcact gcttaaactc cgcgacaata gacttgggaa gcgcacgagg cttcattttg
     5881 agtgggtcaa cgcaaacaac cagaacctct gcttcattca gcaaagtatt ctcggcgttg
     5941 acaatacgtt gcgtgaaaac caaagaggtg ccacgcattg atgttatttc agtctgtatt
     6001 tcgagcatat cgtcgagccg cgcaggtgcg taatattcca ccgtcatttt acgtaccaca
     6061 aaggcaacgc gttcagccat cagcgcctgc tgactgaagt gatgatgacg cagcatctct
     6121 gtgcgtgctc tttcataaaa agcgacgtaa ctggcgtggt acaccacacc accggcatcg
     6181 gtatcttcat agtagacgcg aaccggccat cgaaacagcg ttgtattcac tttacatccc
     6241 ggtaatgcaa caaaagttag agcttttaaa cgctgctact atacgcgcgc gattgaggtt
     6301 tgggaatgag ggcaagttaa gggcgcgtaa aaatttatgg gcctggagcg gcccataatt
     6361 ttttaaagag aattctgatt taaaagaaga agaaaatcag cccgacaatg agaacaatat
     6421 cggcaagcaa tgggcaaaaa atcccttgcc aaagaacctt ctgcggacga aagcccacgc
     6481 cgtgaatcac accagcacag acggcccaca tcagcaataa accatgccag atttccagtt
     6541 cactggtctt cgcggcaaaa cgagatgggt cccaaaacat acatcctgct aacagaagcg
     6601 ccatcacgaa ggaaagcgcc cgcagggggc gcttgtccat taccgcatac aaggtcgcga
     6661 taatcttact catcagatgt cttcttgacc ggctttgcct gattcgacgt gttcaagcgc
     6721 cagcgcggtg attaccccaa acgaacaggc aagaagcgtt cccagaatcc atgcgaaata
     6781 ccacattttt agctccttac ttagtacaga gagtgggtgt tacgttcaat atcttctttg
     6841 gtgatacgac cgaacatttt ccagtaacac caggcggtgt agagcagaat gatcggtacc
     6901 agaaccaccg caacccaggt catgacgtta agcgtcagct ggctggaagt tgcatcccac
     6961 attgtcagac ttgcgttcat catggtgctg gacggcatca caaacgggaa cattgcgata
     7021 ccggctgtca ggatgatgca ggccagcgtc agggaggaga acacaaacgc ccacgcggct
     7081 ttatccatac gtgcagtcag gatggtcagc agcagcagaa ccacacccag tgccggaata
     7141 gcccacagaa ttggcgtgtt gttgaagtta accagccatg cgccagcttc acgaaccact
     7201 tctttattca gtgggttaga ggctgcgtaa tggtccattg tcgatttcac gacataacca
     7261 tcgataccgt acatcaccca tacgccagcc agtgcgaaac agaccagtgt caccagcgca
     7321 gccacctgag ccgttgcacg ggtacgcagg tgcagttcgc ccacggtacg catttgcaga
     7381 taggttgcgc cctgagtaat gatcatccct acgctcacca cgcctgccag caggccgaac
     7441 gggttaagca actggaagaa gttaccggtg tagtacagac gcagatattc atcaacgttg
     7501 aacggtacgc cctgcaacag gttaccgaac gctacaccaa ttaccagcgg cggaacgaag
     7561 ctaccaatga agatgcccca gtcccacatg ttacgccagc gggtttcttc aatcttggag
     7621 cggtagtcaa aaccgaccgg acggaagaac aaagacgcca gcacgaggat catcgccaca
     7681 tagaagccgg agaacgcagc ggcatagacc atcggccagg cagcaaagag tgcgccgccc
     7741 gcggtgatca gccaaacctg gttaccgtcc cagtgtggtg caatggagtt aatcataatt
     7801 cgacgctcgg tgtcgttacg accgaggaaa cgggtgagca tgcccacccc catgtcgaaa
     7861 ccgtcagtga ctgcaaaacc aatcagcaga acgccaacca gcagccacca gataaaacgc
     7921 aatacttcat aatcgatcat ttgacgactc ctgtcttagc gtgccggctg agtagtcgtg
     7981 gaagactgct caaagtgata gcgaccggtt ttcaggctgc ttgggccgag gcgtgcaaac
     8041 ttgaacatta agaacaattc tgccaccagg aacagggtat acaggccgca aatcagcacc
     8101 attgagaaga tgagatcgcc tgcggtcagt gacgaattcg ccacagctgt cggcagcact
     8161 tcaccgatag cccacggttg gcggccatat tcagccacga accagcccgc ttctacagca
     8221 atccacggca gcggaatacc gtacagcgcg gcgcgcagaa gccatttttt ctcgccaatg
     8281 cggttgcgga tgacactcca gaaagagagc gcgatgattg ccagcagcag gaagccacac
     8341 gccaccatga tacggaacgc aaagtacagc ggcgctacac gcgggatgga gtctttggtt
     8401 gcctgttgaa tctgcgcttc agtcgcatca gccacgtttg gcgtatagcg tttcagcagc
     8461 agaccgtaac cgaggtcttt cttcatgcta ttgaactggt cacgaaccgc ctggtcggta
     8521 gaaccagaac gcagttgttc gagcagagag tacgccttca tcccgttacg aatgcgttct
     8581 tcatgctgca ccatcagctc tttcaggccg ataaccgggg tatccacgga acgcgttgca
     8641 atgatgccca gtgcgtaagg gatctgaatc gcaaatttgt tcgtctcctc ttcctgatca
     8701 ggaatgccga acaaagtaaa ggcagcaggc gcaggttgcg tttcccactc ggcttcaata
     8761 gcagccagtt tggttttctg cacgtcgccc atttcgtagc cggattcatc acccagaaca
     8821 ataacagaca gaacagcagc cataccgaag ctggcagcga tagcaaagga gcgtttagcg
     8881 aaggcgaagt cacgaccttt cagcatatac catgcgctga taccgaggat gaacatcgcg
     8941 ccagtcacat aaccagacgc tacagtgtga acgaatttca cctgagcaac cgggttaagc
     9001 accagctcgg agaagctcac catctccata cgcatagttt caaagttgaa atcggacgcg
     9061 attgggtttt gcatccagcc gttcgcaacc agaatccaca gtgcggacag gtttgaaccg
     9121 agcgccacca gccaggtgac acacatatgc tgaactttac ccagacgatc ccaaccgaag
     9181 aagaacagac ctacaaaggt ggattcgagg aagaaggcca tcagaccttc gattgccagc
     9241 ggcgcaccga agatatcccc tacatagtgg gaatagtaag accagttagt cccgaactgg
     9301 aactccatgg tcagaccggt agccacaccc agagcgaagt tgataccaaa caacttgccc
     9361 cagaacttgg tcatatcttt ataaatctgt ttgccggaga ggacgtagac cgtttccata
     9421 atggccagga ggaacgccat accgagcgtc agtggcacaa aaaggaagtg gtacatcgcg
     9481 gtcaaggcaa actgtaagcg cgacagttcg actatatcta acatcatgac tccttgctca
     9541 tcgcatgaag actccgagag tgaaccccgt tagaaagggt cacacgcatg ccccaataga
     9601 aatttgtttg aacactttca tcataccatg tgaatgcaca caaaaacggc gtaaagaaag
     9661 gttgcaaaca tgttaataaa aactcaaatt gatcccacgt atatattacg ccgcaaaatc
     9721 cttacaataa acaggttttt gttgaagcaa atttgcattt ttcgacggtg atcaatttat
     9781 agctaaattg ccgcctttca gccaatttga tcgagaacaa tttatctctt tttgatgccc
     9841 atttccaaga cttatacatt gataaatatc aatttctaca aattatgtta atcatgtttc
     9901 aacaaggtaa ctttagtgat atttatgttt aaaatgtgtg aattataaga ctgaaattag
     9961 tttcggttgg caattaaaga taaaaagaga cgaagcaaaa acagcacggt tgaccttaat
    10021 tctttttttg gtcactggct aatacgacgc agagtttcag gattattaat aaaggcataa
    10081 atctcccccc cgccgatatt aacatttaat taacttataa ttatattata attaactcac
    10141 ccgtggttat tattttgtta aaaataaagc cacatatacg tagcttgaga gtgaagataa
    10201 acacaaaatt acctgatgcg ctacgcttgc cagtgtctac atcatctctg caatatattg
    10261 aatttgcctg cttttgtaga ccgaataaag cgttcatgcc gtatccggca tgaacaaagc
    10321 gccctttgtc aacaatctgg ccgcgcatat gtggcttcgg tttgattgct attcaggcaa
    10381 gccggtaact gaacgtccgt gactggccgg gtaaaaaagg ccccgataga tttgaacctt
    10441 gattttcctc ggtggtaatg tgttcatcca tcatcgtttc tgagcaagaa atcacctcgc
    10501 gactgaaagc aacagtcgca tcacaggttg ctgattcagc cggattaaac agccgtaaaa
    10561 tcacttcttg tcggtcttca gctttcttaa gtgcgcttat caggcatccc actgggggca
    10621 ttttcaacaa actataactt tccggcacgt tgaatccggc tttgttgagc ttcatcgcat
    10681 cccataggat tttgttgtaa cactgtactg gagtcagcca tgctcgcgcc tgctgagcta
    10741 caccagcggc ggttggcgta ccggtataac tcaataaact taggcgacaa gaaagcagac
    10801 cacgtagttg tgagtccggg actggcattt taattcccga aggccgccta ggccttaaaa
    10861 gcagatcttc tttccccagt aagcccacgc cgcgcagcaa cgtaatggca aaggttttct
    10921 tctcttcacc gatgacttca aattcacgta gcccttcgct aaagacagcc atgccgttac
    10981 gcccttcctg taaggctgca tagttgagca tattccatac cggaaccggc gcttctttcc
    11041 agccttcttg ctgccagttg ttcattgcac tgtcgttcac ggggcgcgtt agcgaaccaa
    11101 actgggtatc tgccagaaca ctatcggtgt taaaagatgt agggatcagg acgcgaaggc
    11161 gatgatcgtc agcctggtta tcaaggttga tatccacatc aatgcgcctg ttgttatgac
    11221 taagagtgac aaccaactcg acatctactc tgccagtgga ttgtcttgcg ctgcgttgtt
    11281 gcagattacg cggcaccgcc atttcatagc ggataacagc cctgctctgc caggcctcat
    11341 gaataatctc gcattgcggt ttcgcattcg ccgaagtaat tacccactct tcttttgcgg
    11401 gtgaatagtc atattcatca ccatcatcag agctttcttc aatttgcaac acccggtcat
    11461 agcgcacacc gctatcttta tctaccagtt gtagcgttcc atcctcattg agcgcaattt
    11521 gccagaaagc attttccagt atcccttcag cgtcactttt tgccgcaatc acgttgccag
    11581 gctgattcgc ttcgatataa agcgtgcgat agcccataga agggacaatc tggttgatct
    11641 gtatatcaaa ctccataaag ggatcgtaat taccgtaatg aactatttgc cgatcgatta
    11701 ggcctggatc gatctcacgg gcatggcgaa taaaatacgg tacaggctga ccgcgatcgt
    11761 ctcgtagatt aaactggctg gcgcgcagcc gcacagtggt gttgatcact tcttcccgcg
    11821 gccagggcat caggttaaac aggacgagtt tgtcggcgtc gctctgcggc atgttgtcgg
    11881 cgattttacg catatagaaa cgcagcagat tatccgccat gtcttcagcc agttcgaagc
    11941 gggcgacgat ttcgcgatga actttgtcac tacagcagca gccgatgctg tcgtgggcat
    12001 gattttttaa gatctctttc cacattttct ccagcaagcc gtggtggtat ccaaaaccca
    12061 acgtcctggc cagcgttgcc agcggttcca gcagattaac aatcttattt tcaatacgtg
    12121 cgtgggcaac tttgatatcc atgcgcgtgg aaccgatggt gcgatgcacg cgcatatatt
    12181 tgccatcaat aaattcccct ttcagggttg ccagattatc tcgctgcgct tcgatcttct
    12241 caaatacctc ttcaaagcgg ctcatcacaa actttcgttg aggataaatt tcgcgcagtt
    12301 tctccatcac ttcgaagata ttttgctgca atggcatctg atcatgccca ttcggcagca
    12361 aaatctcttt ggttacagac gctttttcca gcacgtcaaa ataactgtcg aggcgtttgc
    12421 gtaatccgtt ttcgtccgca ggtaagtact taccgatggc ataacccaac ggcatcacct
    12481 gcgccgtcac ttcgctaccg tcactgcttt gccacaaaaa ctcggtttta tccgtaccgt
    12541 ggcgctcaga acatccacgc cagaacatgg tgcaggtaat gccaaatcca ttgtagatat
    12601 gcggcagttg cccggacatg ccaaaggaat cgggtaaata acctattttc atcggctcgc
    12661 caaacgcgag gcagtcacgc attccgtaca tcagattacg gacgatggat tccgcagaaa
    12721 caatcgtggt atcggtctgg gtataccagg ggccgataat caacttgccg gcttctacca
    12781 gtttcttcac gcggtctttg ttttccggtt tcaccgcgaa ataatcttcg aggatcgccg
    12841 tttgcccgtc gagtacgtaa tatttgtatt cgttgtcctg ttccagtcgg cacaggatct
    12901 cttccatatt attgaccagc agaatgcgtg actcttcggt ggtgaaatac cactcccgat
    12961 cccagtgcat atgcggggtg atgtgaacgc gagataccgc tttcatcgtc gtttcctttt
    13021 tgtttatggc attacgccat cagtcagata gttgccatgc ttaaccgcgt gacgccgcca
    13081 gatcaggaga attgcagtcg agattgcagc ccccaccaat gccgcaccaa accagccaat
    13141 tgccgccata acaccgcccg cgccattatc atgaagtaaa aagagcgaga aaatgccagc
    13201 accgggtgtc gaaaggccga tattcatcgc accgacaata gcgcccgtta ccatagagcc
    13261 cagcacaaac gaaccaataa cccgcagcgg atcttcaatc gccatcggga tcgccccttc
    13321 ggtaatacct gccagcccta acagccaggt ggatttcccg gtctcaattt caaactcttt
    13381 aaacaggcgc ggtgcgagca tcgtggaagc ggttacggta aatgccgaaa ccattttgac
    13441 ggaggcgaaa atggcatacg ggccgtaaac gccgttcgcc attgcgccca ggcagaatgc
    13501 ataagcggct ttattcactg gccctccaag gtcaaaggaa cacataaaac cgagaatggc
    13561 acccagcaac agcgcgttac ttcctgacag accgttcagc caggcggtaa gcgagttatt
    13621 gatccaggcg acaggttccc ccaccacaaa cagcatcaga ctgcccgctc ccaatgtacc
    13681 gagcaccggg tagagataaa aagtcaggaa gccattgaat ttactgctaa gacgcaagtg
    13741 atttttcatc cagcgtatca agtaaccggc tatcaatccg ccaacgaccg cgccgagaaa
    13801 cccggagccg atcatgttgg cggcaagtcc agccgcaaag cctggcgcta acgccggttt
    13861 atctgccagc gaataggcgg tataggctgc cagcaccggt accatcaaaa ttccgagcag
    13921 cccgccgccc agtttgcggt acatccacag ccaggagttt tcttcattaa acagatcttg
    13981 tagcccgaag atttgcgaca gtaataccgc gaccgccagc accgtccccc ccgcgacaat
    14041 caacggtacg gcaaaagaga tcccgctcaa cagtgcctgt ttcagctccg ttttgacgct
    14101 tttcaccggt tgcgtatctt gctgtaaggt acgcgtctca tcgccacgct cgagggtaag
    14161 cgattgttgg atcaacgctt ctgcatggcg aatcggctcg gcaacaggca cagaaagcgc
    14221 aggaatgccg ttaaaacgct cactctcctt gatggcgact tcagccgcaa aaatacaggc
    14281 agttgcacta ttgagttgat ccgccgttaa acgcccttca ataccgttag caccttgctt
    14341 ttcaacgtag acattcacgc cgagtttgcg tccggctttt tccagatact ccgcagccat
    14401 gtaggtgtga gcaatacccg ccgggcaggc cgttacgcaa acgatagttg gcgcgttgga
    14461 aaaagaggca gaaggttgcg tgcctccctt gtcatccagc gccgagagca attcatcagg
    14521 cgtcgtcgcc gactgtatac gcgcccgaat ctcatcatcc gccaggcgcg tggtcagcgc
    14581 tgtcagcagt tgcatgtgcg tagtacccgc ttcattgagg ggaatcgcca gcaacaccac
    14641 taaatcaacc gcttccgggc catccacgcc ttcccactga agcggctcgc tgagtgtggc
    14701 gaccgcaaac gccgcttctt ttaccgcagc agttttgcca tgcggcacag ccaacccttc
    14761 acttaaggcg gtcgggccaa ggctttcacg gcgatacact tctttcagaa attgctcagt
    14821 actggaaatt ttccccagag cagcaagacg ttgagtcaac gcgtgaatgg cctcttcacg
    14881 gctggtaaag cgcgcattca gacacaacgc atcgcggtgg gtcagagtcg tcaggttcat
    14941 catgatcctc acttgatcgt ttttatagtc gcgccagtgt gcccgacaaa acaataccat
    15001 tgcgtaatca atttcacttg attgaaatta tttgcatttt atttgtattg tttacaggat
    15061 gaatttatcg ggcataatgg ctaaattcgt attaatgaga tgctaaataa tgggacacaa
    15121 acccttatac cgacagattg ctgatcgcat tcgtgagcaa attgcgcgtg gcgagcttaa
    15181 acctggcgat gcgttaccta ccgaatcggc attacaaaca gagtttggcg tcagccgggt
    15241 tacggtgcgt caggctttgc gccagttagt cgagcaacag atcctcgaaa gcattcaggg
    15301 cagcgggact tacgtcaaag aagagcgggt caattacgat atttttcagt taaccagttt
    15361 tgatgaaaaa ctgtcggacc gtcacgtcga tacacacagt gaagttctga tatttgaagt
    15421 gattccggct gacgattttc ttcagcaaca gctacaaatc accgtgcagg atcgtgtgtg
    15481 gcatgtgaag cgcgtgcgct atcgcaagca aaagccaatg gcgctggaag aaacctggat
    15541 gccgcttgcg ttgttcccgg atctcacctg gcaggtcatg gaaaattcga aatatcactt
    15601 tattgaagaa gtgaagaaga tggttatcga tcgtagcgaa caggaaatca ttcctctgat
    15661 gccaaccgaa gagatgagcc gtctgcttaa tatcagccag acaaagccga ttcttgaaaa
    15721 agtatcgcgt ggatatttag ttgatggtcg ggtgtttgaa tatagccgca acgcttttaa
    15781 taccgatgac tataaattta ccctgatagc gcagcgaaaa tcatcgcgat aagcacaaaa
    15841 aggccatcat acgatggcca accatgtcga aaccgacagc ccttcgccat tcctgtagag
    15901 ggaagcggcg agggctattt cttattacag atatttattt cagaacagtt ttcagtgctt
    15961 caccgatatc cgccaagctg cgaacggttt tcacgcctgc ggcttccaga gcagcgaatt
    16021 tctcatccgc agtccctttc ccaccggcaa tgatcgcacc cgcgtggccc atacgtttgc
    16081 ctttcggcgc agtcacacca gcgatgtaac ccacaactgg cttggtaaca tgctctttga
    16141 tgtacgctgc cgcttcttct tcagcgctac cgccgatctc accgatcatc acgatcgctt
    16201 cggtctgcgg atctttttcg aacatttcga gaatgtcgat aaagttagag ccagggatcg
    16261 ggtcaccgcc gataccgaca caggtcgact gaccgaaacc gtaatccgtg gtctgtttaa
    16321 ccgcttcata ggtcagtgta ccggaacggg aaacgatacc cactttaccc ggtttgtgaa
    16381 tgtgacccgg ctggatacca attttgcatt cacccggggt gataacgcct gggcagttcg
    16441 ggccgatcat acgaacgcct gcttcgtcca gtttcacttt cacggtcagc atatccagcg
    16501 tcgggatgcc ttcagtgatg gtgataatca gtttgatgcc tgcatcgata gcttccagaa
    16561 tggagtcttt gcagaacggt gctggtacgt aaataacaga cgcggaagcg ccagtagcag
    16621 caacagcttc acgcacggtg ttgaacaccg gcaggccgag gtgggtagtg ccgcctttac
    16681 ctggagttac gccaccaacc attttagtgc cgtatgcaat ggcctgttct gagtggaaag
    16741 tcccctggct accggtaaag ccctggcaga taaccttggt gtttttatca attaaaatgg
    16801 acattatttc ccctccactg cggcaacaac ctgctgagct gcatccgtca gaccttttgc
    16861 tgtaataata ttcaggccgc tgtcagccag tttcttcgcg ccgagttcgg cgttgttacc
    16921 ttccagacgt accacgaccg gtacgttaac acccacttcc gctaccgcgc cgatgatacc
    16981 gtcagcgatc aggtcgcaac gaacgatacc gccgaagatg ttaaccagaa cggctttcac
    17041 tttgtcgtca gagaggatga ttttgaacgc ttcggttaca cgttctttgg ttgcgccgcc
    17101 gccaacgtcc aggaagttag ccggttcgcc gccgtgcagt ttaactatgt ccatcgtacc
    17161 catcgccaga cctgcgccgt taaccataca accgatgtta ccgtccagcg caacgtagtt
    17221 cagttcccac tgtgcagcct gtgcttcacg cggatcttcc tgcgactggt cacgcatttc
    17281 gcgcagatca ggctggcgga acagggcgtt gccatcagcg cccagtttgc cgtcgaggca
    17341 aatcagatcg ccctgtttgg tgatgaccag cgggttgatt tcgatcagcg ccaggtcgcg
    17401 ctccaggaaa atggtcgcca ggcccatgaa gattttggtg aactgctgaa ccagtttacc
    17461 ttccagaccc agtttgaacg ccagctcgcg tccctgatac ggcatcgggc cagtcagcgg
    17521 atcaagcgca actttatgga tcaggtgcgg agtttcttcc gccacttttt cgatttccac
    17581 gccgccttcg gtggaggcca taaagaccac gcgacgggaa ctacggtcta caacggcacc
    17641 gagatacagc tctttagcga tatcggtcgc cgcttcaacc agaatctggt taaccggttg
    17701 gccattggca tctgtttgat acgttaccag acgcttgccc agccagtttt ctgcaaaagc
    17761 acggatgtct tctttgctgt ttacaacttt cacaccgccc gctttaccgc ggccaccagc
    17821 gtgaacctga catttcacta cccacggacc ggcaccgatt tttgaagcgg cttcttctgc
    17881 ttcgcgcgga gtagtacagg cataacccac cggtgctggt aagccatagc gggcaaacag
    17941 ttgttttgcc tgatattcat gtaagttcat gtgttctgtc catccttcag taatcgttat
    18001 cttttaaacc gtagaccgac accctatcca cgtcggtcta cagtgcaggt gaagcttaaa
    18061 ctactacacg tccagcagca gacgcgtcgg ctcttccagt aactctttga tcgttaccag
    18121 aaagcccacg gattcgcgac catcgatcag acggtgatcg taggacagcg ccaggtacat
    18181 catcggcagg atctcaacct gaccattcac cgccatcgga cgatctttga tagcgtgcat
    18241 acccagaatt gcgctctgcg gcgggttgat gatcggcgta gacatcaggg aaccgaacac
    18301 accaccgttg gtgatggtga agttaccacc ggtcagatct tcaaccgtca gcttgccatc
    18361 acggcctttg actgccagct ctttgatttt cttctcgatg tctgccatgc cgagggtatc
    18421 gacatcacgc agaaccggcg tcaccaggcc gcgcggcgta gaaaccgcca tgctgacgtc
    18481 gaaatagttg tggtaaacca cgtcatcgcc gtcgatagac gcgttcactt ccgggtaacg
    18541 tttcagggct tcaaccaccg ctttcacgta gaaggacata aagcccagac ggatgccgtg
    18601 gcgtttttca aacgcttcac cgtactgctt acgcagatcc ataatcggct tcatgttgac
    18661 ttcgttgaac gtggtcagca tggcggtgga gtttttcgct tccagcagac gctctgccac
    18721 acgcttacgc aggcgagtca tcgggacacg tttttcacta cgtgcagcca gagccggttg
    18781 cgccgccgga gcagccgctg ccggagcaga ctctttcgcc ggggctttcg ccagatgttt
    18841 ttccacatct tcacgagtca gacgaccacc cacaccggtg cctttaatgg cgctggcgtc
    18901 gagattgtgt tcagccagca ggcgacggat cgccgggctt aacgcatcgt tgttttgctc
    18961 ttccagagac gcctgctggc gttgcgccgg agtggacgct ttctcttcag atttggcgct
    19021 ggtttcttta ccggcgctgt tgccttcacg caggcgacca aggatctgac gagacgttac
    19081 cgttgtacct tcatcttcca gaaccgcatc cagaatgccg tctgctgatg ccggtacttc
    19141 cagtaccact ttgtcagttt cgatttctac cagcacttca tcacgtacga ctgcgtcgcc
    19201 gggtttttta tgccaggttg cgacggtggc atcggctacg gattcaggca ggtcagggac
    19261 cagaatatct acgctactca ttgtgtatcc tttatttatt cgacgttcag cgcgtcatta
    19321 accagatctt gttgctgttt ctggtgaacg gacatatacc ctaccgccgg agaggcggag
    19381 gccgggcggc ctgcataacg cagagaagcc ccaaacggaa tcacttcacg gaaatgatgc
    19441 tggctgcagt accatgcgcc ctggttgagc ggctcttcct ggcaccagac aaaatccttg
    19501 acgtgagcaa actgctgcaa cacttcctgc atcgctttat gcgggaacgg gtagagttgc
    19561 tcgatacgca caatggcgac atcgtgttga ttgttcttac gacgctgttc cagcaggtcg
    19621 taataaacct taccagaaca catcactacg cgcttcacgc ccttcggatc aagctcgtcg
    19681 atttcaccga tggctggcag gaaggtgccg ttcgccagtt cttcgaggct ggaaaccgcc
    19741 agcggatgac gcagcaggga tttcggcgac atcacgacca gcggacgacg catcccgcgc
    19801 agcgcctgac gacgcagcat gtggtaaacc tgtgccgggg tagacggtac gcaaacctgc
    19861 atgttttgct cagcacaaag ttgcagataa cgttccagac gcgcggagga gtgctccggc
    19921 ccctgccctt cgtaaccgtg cggcagcaac atcaccagac cacacatccg gccccatttc
    19981 tgctcgccag aggagatgaa ctggtcgata accacctgcg caccgttggc gaagtcaccg
    20041 aactgcgctt cccagatggt cagggtgcgt ggttctgcgg tggcataacc atattcaaac
    20101 gccagcacgg cttcttctga cagtacggag tcccagacac ggaacgcgcc ctgaccgtta
    20161 tggatgtgtt gcagcggcgt gtaagtggaa ccgttagact ggttgtggat caccgcgtgg
    20221 cggtggaaga aggtaccgcg accggagtct tcacccgaca ggcgaaccgg aatgccttca
    20281 tcaaccagcg tggcgtaagc gaggttttcc gcaccgcccc agtcgaacag tttctcaccg
    20341 gctgccatcg cctggcgatc gccataaatc ttggcaacgc gagactgcat ttcaactgct
    20401 tctggtaccg tactgatacg tttagccagt tcctgcaggc gcttcatctc aactttgttc
    20461 gggtactctt cgtcccattc gtggttgagg tacggcgacc aggtgaaaga gtgcatgttc
    20521 atcggacgcc actctgccac tacgcaatcg ccagcatcca gcgcatcgcg gtacaggtta
    20581 accatctcgg tggcatcttc cagcgtcgcc actttttcct gctccagctt gtcagcgtag
    20641 attttgcgcg gtgtcggatg ttttttgatt ttctgataca tcagtggctg ggttgcgctc
    20701 ggctcgtcgg cttcgttgtg gccgtgacgg cggtagcata ccaggtcgat gaagacatca
    20761 cgtttaaagg tgttacggaa atcgagcgcc agacgggtca caaaggcaac ggcttccgga
    20821 tcatccgcgt taacgtggaa aatcggtgcc tgaaccatct taccgatatc agtacagtac
    20881 ggtgtagaac gggcatccag cgggttagag gtggtgaaac caacctggtt gttgataacg
    20941 atacgtaccg taccgccaac ttcataacca cgcgctttcg acatgttcag agtttcctga
    21001 accacgccct gcccggtcac tgcggcgtca ccgtggatgg tgattggcag cactttgttg
    21061 ctgttcggct catcaagtct gtccagacgg gcacgaacag agccgataac gaccgggctt
    21121 acaatctcaa ggtgagacgg gttaaacgcc agcgccaggt gcaccaggcc gccatcggtc
    21181 tggaagtcag acgagaagcc catgtggtat ttcacgtcgc ctgtgccgag gtgttcttta
    21241 tgtttaccgg cgaactcgtc gaacaagtct tgcggttttt tacccagcac gttcaccagc
    21301 acgttcagac gaccacggtg cgccatcccg agaaccactt cgcgggtgcc gctgttgcca
    21361 gcgtggcgga tcatctcttt aagcatcggg attaacgcgt caccgccttc cagcgagaag
    21421 cgttttgcgc cagggaattt tgcgccgagg taacgctcaa ggccttcagc ggcggtcagt
    21481 tcgcttaaga agcgtttttt ctcttcgcta ttgaaagtcg cgcgaccaga ctcgatacgc
    21541 tgttggatcc agcgtttttc ttcggtgctg gtgatgtgca tatactcggc accaatcggg
    21601 ccgcagtagg tttgcttgag ggcggaaata agctcgccga gtttcatggt ttctttgccg
    21661 ctggcaaatg aaccgacgtt gaaggtttcc tggaagtctg cttcggtcag atcgtggaaa
    21721 gacggatcca gatcggccac tttatcttgc tgccacagtc ccagcggatc tagattcgca
    21781 tgctggtgac cacggaagcg gtatgcgtta atgagctgca ggactttaac ctgcttcaca
    21841 ttggtgtcag ggtcggagat cgttgaagag taacgtgaag cgtctttcgc caggcggcgg
    21901 aaatattcac gcgtttgaga gtggaattga tccggtttga ctcccgtacc aggtaactgc
    21961 tggaacgtcg aacgccagtt agcgtcaacc gagtcaggat cggttaagaa gtcttcatag
    22021 agctgttcta tccagctctg gtttgcgcca gagaggtaag aagagtccaa ccaggctttc
    22081 aaagcgctgt tctgcatcgt gatcccttaa gcatcttttt tatgcttact tcgccgtgga
    22141 tactaccacg cacagtgatg tgcgtgccgg ggttcacttg cgagctcttg tctacgtagt
    22201 ggctcgcgaa ggaaccttta aaaactgtct tagtgtcagg ggcagttttt aaagatttcc
    22261 tgcatttctt tgttacgcct gatgcgctgc gcttatcagg cctgcgggta atgacttgta
    22321 ggccggataa ggcgttcacg ccgcatccgg cactggttgc ctgatgcaac gcttgcgcgt
    22381 cttatcaggc ctacagttta cgcattacgt tgcaacaaca tcgacttgat atggccgatg
    22441 gcgcgcgtcg ggttcagccc cttcggacat acactgacgc agttcatgat gctgtgacag
    22501 cggaatacgc tgaatgcatc gctcaaaccg tcgaggcggc tgtcagtctc ggtatcacgg
    22561 ctatcgatca ggaaacgata tgccgctaac aagcctgccg ggccgataaa cttatcggga
    22621 ttccaccaga aagacggaca agaggttgaa caacatgcgc agagaataca ttcatacagc
    22681 ccgtcgagtt tttcgcgctg ctctggcatc tgtaaatgct cgcgagctgg cggattttgt
    22741 ccattattca acaggtaagg cttaattttc tcatattgcg catagaattg tcccatgtct
    22801 accaccaaat cgcggatcac cggtaaacct ggcagcgggc gaatcacaat cttcttgccc
    22861 ggctggttga gtgccgaaat cggggtaata caggccagac cattcttgcc gttcatgttc
    22921 agaccgtcgg aaccgcacac accttcacgg caggagcggc ggaacgacag gctgggatct
    22981 ttctctttca gctggataag cgcatccagc agcatcatgt cgcgaccttc ttccgcttcc
    23041 agggtgtaat cctgcatacg cggagcatca tcaacatccg ggttatagcg ataaattgaa
    23101 aactcgagtc tcattttcct gtctccgcat tagtaagtac gaatcttcgg cgggaatgcc
    23161 gggcgcagtt tcggttccat gttgacgctt cggcgcgtca tggattccga ctcaggcaga
    23221 tacagggagt ggcacagcca gttttcatca tcacgatccg ggaagtcgaa gcggctatgt
    23281 gcgccacggc tttcggtacg gaagttggca gaaacagccg ttgcatacgc cgtttccatc
    23341 aggttatcca gttccaggca ctcaacgcgc tgggtgttga actcgctgga agtgtcatcc
    23401 agacgggcat ttttcagacg ctcgcggatc actttcaact gctcaagccc tttcgccatc
    23461 gcatcacctt cacggaagac cgagaagtta tgctgcatac attcttgcag cgctttacgg
    23521 atcgccaccg gatcttcacc gttacgatta ttgttccacc ggttcaggcg atccagagac
    23581 gcttcaacat cagactcgct ggcatcgtgc agtgcgccct gctcggcgat agactcttgc
    23641 agatgcagac ctgccgcgcg accaaagacc accaggtcca gcagcgagtt gccgcccaga
    23701 cggttagcgc cgtgtaccga tacacaagcg atttcaccaa cggcaaacag tcccggaaca
    23761 accacatctt cgcctttctc attcacagtc agcgcctgac cggtaacttt ggtcggaata
    23821 ccgcccatca tgtagtgaca ggttgggata accggaatcg gctctttcac cggatcgacg
    23881 tgagcgaagg tacgggaaag ctccaggata cccggcagac gggattcgag aacttcttta
    23941 cccaggtgat cgagtttcag tttcgcgtgt ggcccccacg gaccatcaca gccgcgacct
    24001 tcacggattt caatcatgat ggaacgcgcc accacatcac ggcccgccag gtctttggcg
    24061 ttcggcgcat aacgctccat aaagcgttcg ccatgtttgt tcagcagata accgccttca
    24121 ccacggcaac cttcggtgac cagtacgccc gcaccggcaa tgccggtcgg gtggaattgc
    24181 cacatttcca tatcctgcac cggtacgcct gcgcggatag ccatgccgac accgtcgcca
    24241 gtgttaatgt gggcgttagt ggtggactga taaatacgcc ctgctccgcc agtcgccagc
    24301 acggtagcgc gggctttgaa ataaaccact tcaccggttt cgatgcacag tgcggtacaa
    24361 cccaccaccg cgccatcctg gtttttcacc agatccagcg cataccactc ggagaaaatg
    24421 gtggtgtggt ttttcaggtt ctgctgataa agcgtgtgca acagtgcgtg accggtacgg
    24481 tcagcagccg ccgcagtgcg tgccgcctgc tcgccgccga agtttttcga ctgaccgcca
    24541 aacggacgtt gatagatacg accatcatcg agacgcgaga acggcaggcc catgtgttcg
    24601 agttccagaa tcgcttccgg cccggtttta cacatatatt caatcgcgtc ctggtcaccg
    24661 atatagtccg acccttttac ggtgtcgtac atatgccatt cccagttatc ttcatgggta
    24721 ttacccagcg caacggtaat gccgccttgc gcagaaacgg tatgggaacg ggtcgggaag
    24781 actttagaga gcagggcaca ggtctggccg ctctgggaaa tttgcagcgc cgcgcgcata
    24841 cctgcgccac cggcaccaat cacaactgca tcaaattctc tgactggcaa tttcatcaca
    24901 caccccacac cacaacgaat ccataaatca cgtaaaccac cagtgcaacg acaatcacca
    24961 gttgcagcat caggcgcaag gccagcggtt taacgtagtc ggtcaacacc tgccacatgc
    25021 cgatccaggc atgaatcaag atagaaaaca gcgccagcag ggtgaacact ttggtgaacg
    25081 cagaggcgaa gaaaccgatc cagacttcat atgtcagttc gccactggtg gcgaaaaaac
    25141 cgaccatata aatgatgtag agcgtcagga cgatagcggt agcgcgaacg aggatgaaat
    25201 catgtacgcc attgcgtcct aatgcggagg cgttgcttac catacgagga ctcctgcgag
    25261 aagtgaaagc acgacagtaa taacaaagga gattttggcg gagcgtttac ccgcttcgaa
    25321 tgtttcttcc agatagccaa aatccatcat catgtggcga atacctacga cgacgtgata
    25381 cgccagagcg gtaaggatgc cccacatgat aaatttgacg aagaagctgc ccataatcgc
    25441 ggaagcttgc tcgaaacctt caggggaaga gaggctggta cccagaagcc actgcaggat
    25501 gcccactgca acaaaggtga tcacaccgga aacgcgatgg agaatggacg ctatcgccgt
    25561 gatggggaac cggatggtct gtaggtccag attaacaggt ctttgttttt tcacatttct
    25621 tatcatgaat aacgcccaca tgctgttctt attattccct ggggactacg ggcacagagg
    25681 ttaactttct gttacctgga gacgtcggga tttccttcct ccggtctgct tgcgggtcag
    25741 acagcgtcct ttccataact gcgcgtcatg caaaacactg cttccagatg cgaaaacgac
    25801 acgttacaac gctgggtggc tcgggattgc agggtgttcc ggagacctgg cggcagtata
    25861 ggctgttcac aaaatcatta caattaacct acatatagtt tgtcgggttt tatcctgaac
    25921 agtgatccag gtcacgataa caacatttat ttaattttta atcatctaat ttgacaatca
    25981 ttcaacaaag ttgttacaaa cattaccagg aaaagcatat aatgcgtaaa agttatgaag
    26041 tcggtatttc acctaagatt aacttatgta acagtgtgga agtattgacc aattcattcg
    26101 ggacagttat tagtggtaga caagtttaat aattcggatt gctaagtact tgattcgcca
    26161 tttattcgtc atcaatggat tctttacctg caagcgccca gagctctgta cccaggtttt
    26221 cccctctttc acagagcggc gagccaaata acaaacgggt aaagccaggt tgatgtgcga
    26281 aggcaaattt aagttccggc agtcttacgc aataaggcgc taaggagacc ttaaatggct
    26341 gatacaaaag caaaactcaa cctcaacggg gacacagctg ttgaactgga tgtgctgaaa
    26401 ggcacgctgg gtcaagatgt tattgatatc cgtactctcg gttcaaaagg tgtgttcacc
    26461 tttgacccag gcttcacttc aaccgcatcc tgcgaatcta aaattacttt tattgatggt
    26521 gatgaaggta ttttgctgca ccgcggtttc ccgatcgatc agctggcgac cgattctaac
    26581 tacctagaag tttgttacat cctgctgaat ggtgaaaaac cgactcagga acagtatgac
    26641 gaatttaaaa ctacggtgac ccgtcatacc atgatccacg agcagattac ccgtctgttc
    26701 cacgctttcc gtcgcgactc acatccaatg gcagtcatgt gtggtattac cggcgcgctg
    26761 gcggcgttct atcacgactc gctggatgtt aacaatcctc gtcatcgtga aattgccgcg
    26821 ttccgcctgc tgtcgaaaat gccgaccatg gccgcgatgt gttacaagta ttccattggt
    26881 cagccatttg tttatccgcg caacgatctc tcctatgccg gtaacttcct gaatatgatg
    26941 ttctccacgc cgtgcgaacc gtatgaagtt aatccgattc tggaacgtgc tatggaccgt
    27001 attctgatcc tgcacgctga ccatgaacag aacgcctcta cctccaccgt gcgtaccgct
    27061 ggctcttcgg gtgcgaaccc gtttgcctgt atcgcagcag gtattgcttc actgtgggga
    27121 cctgcgcacg gtggtgctaa cgaagcggcg ctgaaaatgc tggaagaaat tagctccgtt
    27181 aaacacattc cggaatttgt tcgtcgtgcg aaagataaaa atgattcttt ccgcctgatg
    27241 ggcttcggtc accgcgtgta caaaaattac gacccgcgcg ccaccgtaat gcgtgaaacc
    27301 tgccatgaag ttctgaaaga gctgggcacc aaagatgacc tgctggaagt ggctatggag
    27361 ctggaaaaca tcgcgctgaa cgacccgtac tttatcgaga agaaactgta cccgaacgtc
    27421 gatttctact ctggtatcat cctgaaagcg atgggtattc cgtcttccat gttcaccgtc
    27481 attttcgcaa tggcacgtac cgttggctgg atcgcccact ggagcgaaat gcacagttac
    27541 ggtatgaaga ttgcccgtcc gcgtcagctg tatacaggat atgaaaaacg cgactttaaa
    27601 agcgatatca agcgttaatg gttgattgct aagttgtaaa tattttaacc cgccgttcaa
    27661 atggcgggtt aatttttata tgcctaaaca caaaaaattg taaaaataaa atccactatc
    27721 ggacattcat atattctaaa aaaaaataca tcgatataaa ttataaacaa atagatgatt
    27781 tttaatcatc tatcttcctc tcagttacat gaataaacca ccaaatatag cgcaccccta
    27841 taattcccaa tactttattc atcaaacatt taattcatac catcaattaa ttacttttac
    27901 atttaataaa attaataata tttttaagaa aaactaaaaa cattcgacaa tcaaataaga
    27961 tattcttgca ccgatatact tttttctttt ctgttaaagg actcagttat gtttaaagga
    28021 caaaaaacat tggccgcact ggccgtatct ctgctgttca cagcacctgt ttatgcggct
    28081 gatgaaggat ccggtgaaat tcactttaaa ggtgaagtta ttgaagcacc gtgtgaaata
    28141 catcaggatg atattgataa agaggttgaa ctcggtcagg tgaccaccag ccacattaat
    28201 cagtcacatc acagcgatgc cgttgctgtc gacctgctct tagtcaactg tgatctggaa
    28261 aactccagca acggttccgg tggcaagatt tctaaagttg cagtcacctt tgatagctca
    28321 gcgaaaacca ccggcgcaga tccgattctc aacaacacca gcacgggtga agccaccggc
    28381 gtcggcgtac gtttaatgaa taaagatcaa agcaatatcg ttttaggcac cgctactcca
    28441 gatatcgacc tggctccgac ctccagcgaa caaacgctga atttctttgc ctggatggaa
    28501 caaattgatc aggctacacc tgtaacgcca ggcgcagtta cggcgaatgc aacctacgtg
    28561 ctggattata aataagtatt actttggcgg cgctatcgcc gcctttcata aaatatttat
    28621 caggtatgga caccgtgaat atttatcgac tctcttttgt atcctgcctg gtcgtggcga
    28681 tgccttgcgc attggcggtc gaattcaatc tgaatgttct cgataaatca atgcgcgacc
    28741 gcattgatat ttcattatta aaggaaaaag gagtcattgc tcccggtgag tattttgtta
    28801 gcgttgcggt aaataataac caaatcagta acgagcaaaa gattaactgg cacaaaaatg
    28861 acgataaaac cattccgtgc atcaatgatt tactggtcga taaatttggc ttaaaacctg
    28921 aagtccgtca gtcgttacca ttgataaatc agtgcgtcga ttttagctcc cgacctgaaa
    28981 tgctcttcaa tttcgatcaa gccaatcagc aactgaatat tagtattccg caagcctggc
    29041 tggcgtggca ctcagaaaac tggaccccac cctccacatg gaaagaaggt gtcgccggtg
    29101 tcctgatgga ttacaacttg tttgccagca gctaccgccc acaggatggc agcagcagca
    29161 ctaatcttaa cgcctatgga acagccggaa tcaacacggg ggcatggcgc ttacgcagtg
    29221 attatcagtt gaatcatact gatagcgatg ataaccatga acagtcaggc gaaatatcac
    29281 gtacctatct ttttcgcccg ttaccgcaat taggttcaaa attaaccctc ggcgaaacgg
    29341 attttagtcc caatattttc gacggttttt cttataccgg cgcggcactg gcaagtgacg
    29401 atcgaatgtt gccatgggaa ctacgcggct acgccccaca aattagcggt attgcacaga
    29461 ccaatgccac ggtgacgatc agtcaatcag gccgcgtcat ttaccagaaa aaagtcccac
    29521 caggcccatt tatcattgac gaccttaatc agtctgttca gggcacactg gatgtcaaag
    29581 tgacggaaga agatggtcgg gtgaacaatt tccaggtttc agcagcatcg acgcccttcc
    29641 tgactcgtca gggacaggtt cgctataaac tggccgcggg tcagccacgg ccctccatgt
    29701 cacatcaaac tgaaaatgaa acctttttta gcaatgaagt ttcctggggg atgctgtcaa
    29761 acacctcgct gtacagcggc ctgctgcttt ctggtgatga ctaccattct gccgcaatgg
    29821 gtattgggca aaatatgctg tggcttggtg cgctgtcgtt tgatgtcacg tgggccagta
    29881 gccattttga tactcagcag gacgagcggg gcttaagcta ccgttttaat tacagcaaac
    29941 aagtggatgc cactaacagc acgatttccc tcgccgctta tcgtttctcc gatcgtcatt
    30001 ttcacagcta cgccaactat ctggatcaca aatacaacga cagcgatgcg caggacgaaa
    30061 aacagacgat cagcttatct gtgggccaac cgattacccc actaaacctc aatctttacg
    30121 ccaacctgct acatcaaacc tggtggaatg cagacgcctc cacgaccgcc aacatcacgg
    30181 caggttttaa tgttgatatt ggtgactgga gagatatctc tatttctacg tcattcaata
    30241 caacccatta cgaagataaa gatcgcgaca accagattta cctgtcgatt tcgctcccct
    30301 tcggtaacgg tggtcgggtt ggctatgaca tgcaaaacag tagccacagc accacacacc
    30361 gcatgtcgtg gaacgatacg ctggatgaac gtaatagctg gggcatgtct gccggactgc
    30421 aatccgaccg tcctgacaat ggagcccagg tgagcggtaa ctatcagcac ctgagttcag
    30481 cgggtgagtg ggatatttct ggtacctatg ccgccaatga ttacagttcc gtcagcagca
    30541 gctggagcgg ttctttcacc gcaacccaat atggtgcagc gtttcatcgc cgcagctcca
    30601 ccaatgaacc acgcctgatg gtcagcaccg atggcgtggc agatattccg gttcagggca
    30661 atctcgacta caccaaccat tttggcattg cggtggtgcc gttgatttcc agttaccagc
    30721 cttccaccgt ggcggtgaac atgaatgact tacccgacgg cgtaacagtt gcagaaaacg
    30781 ttatcaaaga aacgtggatt gaaggcgcga taggttacaa atcactggct tcccgttccg
    30841 gtaaagacgt taacgtcatc attcgcaacg ccagcggtca gtttcctccc ctcggagcgg
    30901 atatccgcca ggatgacagc ggcattagcg tggggatggt tggcgaggaa ggacatgcct
    30961 ggttaagcgg agtcgctgaa aatcaaaagt ttaccgtggt ctggggtgat agccagcatt
    31021 gctcgctcca tcttcctgaa catatggaag acaccgcaaa tcgcctgatt ttaccttgtc
    31081 attaattaag gaaatgaaaa tgacatttat gaaaggacta ccccttttgt tgttggttgc
    31141 caatctgtgc agccacgctg cactacaacc cgatcgcact cgtattgtgt ttaacgctaa
    31201 tgataaagct accagtctgc gggtggataa tcgtagcgat aaactcccct acctcgctta
    31261 ttcctggctc gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg ccctgccgcc
    31321 gatacaacgt ctggaaccga aagcaacaac acaggtacgg atagtaaagc aagcctcgac
    31381 cacaaaatta ccgggcgatc gcgaaacgct atttttctac aatatgcgtg aaattcctcc
    31441 cgccccggaa aaaaacagcg accatgccgt tctgcaagtc gccattcaga gtcgtatcaa
    31501 ggtgttctgg cgaccggctg cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt
    31561 acaggtcagc cagcagggca accaactgac actgaaaaat cctaccgcgt attatctgac
    31621 cattgcctac ctgggacgaa atgaaaaagg cgttctcccc ggcttcaaaa ccgtgatggt
    31681 tgcgcctttt agtacggtca acacaaacac cggaaattat agcggcagcc agttttatct
    31741 cggttatatg gatgattacg gcgcgttacg catgacgacg ctcaactgca gcggacaatg
    31801 ccatttacaa gccgtggagg cgaagaaatg agtgctggca agggagtgtt gctcgtcatc
    31861 tgtctgttat ttctgtcgtt gaagtctgcg cttgccctga actgctactt tggttcatcc
    31921 ggtggctcgg tagaaaaatc agaagcgatt caaccttttg ctgtaccagg caatgccaaa
    31981 ctcggcgata agatctggga atctgacgat attaaaattc ccgtctattg tgacaacaat
    32041 accaacggca actttgaaag cgaacatgtc tacgcatggg taaaccccta cccgggggtg
    32101 caggatcgtt attatcaact cggagtaact tacaatggcg ttgattatga cgccaaccag
    32161 ggaaaaagcc gcatcgacac taaccagtgt atcgacagta agaacattga catttatacc
    32221 cctgatggga tggcagaaca aaatttgctc gggtgatccc gctaatattc acatgtcgcg
    32281 aacctttctc gctcgcatgc ggttatacgt caaaatacga gaaatgccgc cgcatgatta
    32341 tcaaagtacg cttagcgact atatcgtcgt gcaatttgac ggtgccggta gcgttaacga
    32401 agaccctact gcccaaaacc tgaaatatca tattactggt ctggaaaaca ttcgcgtgct
    32461 ggattgcagc gtcaattttt ccatttcacc ggaaacacag atgattgatt ttggtaaatt
    32521 taatttgctg gatattcgtc gccacacaat gtcgaaaaca ttcagtatta agacgacaaa
    32581 atcacaaaat gaccaatgca ccgacggatt taaagtcagt tcctcctttt ataccgaaga
    32641 aacgttggtt gaagaagata aagcattgtt gattggtaat ggtctgaagc tacgtttgct
    32701 ggatgaaaac gcctcgcctt ataccttcaa taaatatgct gaatacgccg atttcaccag
    32761 cgacatgttg gtctacgaaa aaacctatac agctgaactt tcgtccattg caggtacccc
    32821 catcgaggct gggcccttcg atacagtggt gctttttaaa attaactata actgatttgc
    32881 catgatgccc tttgcagccg gaaagggcat ctctttcaga aaattgctga ttttcccctt
    32941 ttccttgatt gctgttattt tttcctagcc cggctttctt tatgctgtat cgatacacca
    33001 aagagaaaat aatgagggag cgaaggatgc cagttttgca gtggggaatg ttatgtgtgc
    33061 tatcacttct cctttctatt ggtttcctcg cggtacatct cccggcggcg ctattacttg
    33121 ggccgatgat cgccgggatc atcttcagta tgcgcggaat tactctgcaa cttccccgct
    33181 ccgcttttct cgccgcgcag gccattcttg gctgcatgat tgcgcaaaac ctcactggtt
    33241 cgattctcac cacgctggcc gccaactggc cgatcgtgct tgctatttta ctggtgacgc
    33301 tgctttccag cgccatcgtg ggttggttat tggtgcgcta tagctcactg cctggaaata
    33361 ccggtgcctg gggttcttct ccaggcggcg cagcggcaat ggtcgctatg gcgcaagatt
    33421 acggcgcaga tattcgcctg gtggcgttta tgcaatatct gcgagtgctg ttcgtcgcgg
    33481 gggccgctgt tctggtcacc cgcatgatgc tgggtgataa cgctgaagcg gttaaccagc
    33541 aaatcgtctg gttcccgcca gtgagcatta atctcctgct tacgattttg ctcgcagtcg
    33601 ttgccggtac ggcgggatgc atgctgcgtc ttccttccgg cacgatgctc atcccgatgc
    33661 tggcgggcgc agtgctccag tctggtcagc tcatcaccat cgaactaccg gaatggctgc
    33721 tggcgatggc gtatatggca attggctggc ggattggtct tggtttcgat aaacaaatct
    33781 tactgcgggc actacgcccg ctaccgcaaa tcctgctgtc gatttttgct ctgctggcta
    33841 tttgtgcagg tatggcgtgg ggactgaccc ggtttatgca tattgatttt atgactgcct
    33901 acctcgccac cagtcccggc gggcttgata ccgtggcggt catcgccgca gggagcaatg
    33961 ccgatatggc gcttattatg gcgatgcaaa ccctgcgcct gttcagtatt ttgctgacgg
    34021 ggccagccat tgcgcggttt atttcaacct atgcgccgaa gcggtcggcc tagtgctggc
    34081 agcctggaca ccagtaaaac gggcgcgagg acaacgtggt tttctcaatg atgccgccac
    34141 agcgttcgca cagttcgcca tcacgatgaa aaaccttaaa gcgaaacagc gccccatgat
    34201 gcttattctc atccacctgc ccccgcgtag cgtaagaaaa ccgaggaatc tccagtaacg
    34261 cgtgtgcgag tgcatccagt tgcgccgcat tgagatcttt cgctttatga tttccagtca
    34321 acccaacctg ccagaggatc tccacccgca aataattgcc aagcccagcc agaaacgcct
    34381 gatcgagcag taatcctgca aactgacggt tacgaaagcg cggcgacaat aaccgttcct
    34441 ttaccacctc cggcgtcaga ttcggatcca gcacatcggg accaacgcgt tgtaaaaacg
    34501 gatgcgtggt cagttgttcc ggcctcaaca tctcaatatc cgaggcgcta taaagcagaa
    34561 tggttttgtc agccgtttgc agttttaccc gcaatactcg cgtggtctgc ggctcttcgc
    34621 cggtatcaac cacgcgccag acgccgtaaa gctgattatg gctgtagagc gttaaaccgt
    34681 tgggaaaatg agttaacaac gccttaccac gcgtttccac atgggtaacg tgttgaccga
    34741 taagttgtga ttgataagtt tttaactgcg ggaaggcaaa ccagacatca gttagtggtt
    34801 tgcctttgat cgccgcctcc aggttatccg ctgcacggcg gatctccggg ccttcaggca
    34861 tggtgatatc cttttttcat tctttattaa gcggggttat gctgcgacaa caatcccctt
    34921 tttggcaaat gtagctcgta atcggcgggc gaacgccaga gcgtgctcgc cgtcgccatg
    34981 caggcagacc gtttgcgccg cgaccgttgc ccattcgccg gtgatacttt tgactctgcc
    35041 gtgttgcacc atctccagcg tttgcgccaa cgcctgttct tcgttttcaa tcaacgcgcc
    35101 tggctgactt cgcggcacca gcgagccgtc agcctgataa ccgcgatcgg caaacacttc
    35161 ctcgcgcgtt gtcaggccat attgcttgcc tgcacgaatc agctcgcttc ctgccagccc
    35221 aacgagaacc aatgccggat cgcaagcgta taccgctctg gcgatggcgt ctgccagttg
    35281 tgcttctttc gccgcctggt tgtacaacat gccgtgcggt ttgacatgac gcattacgcc
    35341 gccttgcgca cgggcaatcg ttgccagcgc gccaatttga tacagcgtct gggcgtaaac
    35401 ggtttccggc ggcagctgca tggcgctgcg accaaaattt tccctgtcgg gaaaactcgg
    35461 gtgagcgcca atcgcgacac cattttttat tgcttcacgc acgcaagcct gcatggtttg
    35521 cgcatcgcct gcatgaaatc cacaagcaat attggcagag gaaaccagcg ttaatagctc
    35581 tgcgtcgctg gcgcagcctt cgcccagatc ggcgttcagg tcaattttca ttgtgcagcc
    35641 gccacgctaa ttgttcaaaa taacgttgct gatcttgccg cgctttcagt gcctcttcca
    35701 gtgaacactg gacaaaatga atcggctgac cgagcggaat ttgcgccaga tggtacatat
    35761 cagcctcaat gatacaggca atacgtggat aaccaccggt ggtctgtgcg tcgttcatca
    35821 acacaatcgg ctgcccgtta tgcggcacct gcaccacgcc cggtaacaaa ccgtgagata
    35881 acagttcgcg atcggtgatg cgttttaaaa tttgcccctg taaacgatag cccattcggt
    35941 tactttgcga gctaagctgc cagggcgaac gccagaatgc atcctgcgag gcgcgatcga
    36001 actcatgata ttccggcccc ggcaaggcgc gaatgcggtt gccccacagc agctgtttaa
    36061 cgccctgcgc ttccataaaa tcacgcttcg atttgccaat cgggagtcag tcaccatcct
    36121 tcagtaaacg gccttccagc ccgccaatcc ccactttgag atcggtgctg catgacccca
    36181 ttaccggcgg aacatcaata ccacccgcga ccgccagata actgcgcatc ccgtgctgcg
    36241 ggcgtttaag cgttaaacgc tggcctgctt tcatcggcaa tcgccagccg gtccagacgg
    36301 cgttatcatc caaccgcgct tcgcaacctg cacccgtcag ggcaaaccac ccatcagttt
    36361 cgaactcaac tgttaactga ccgagcgtga tctccagcgc gggggcattg gcgtcattac
    36421 ccaccagcag gttagcaatg cgcagcgcag gcatatccag tgcgccacag tggctgatac
    36481 ccgactggca aaaaccgtga cgaccgccat cctgcacagt ggtgtacatg cccgcacgaa
    36541 taatcttcag catattccct ccttctgcgg tacaaatcgc acgctgtctc ccggacgtaa
    36601 taagatgggt tcgtcacgcg ccggatcaaa caggctgagt gaggtatgac caatcaactg
    36661 ccagccaccc ggcgttgcca gcggataaac accagtctgc ggcccgccga tcccgacaga
    36721 acctgccgga acgagcaagc gcggttcagc gcgccgtggc gtgtgtagtt gttccggcaa
    36781 actcccgaga tacgggaagc ccggttgaaa acctaaaaac cagaccacgt attccacgga
    36841 ggagtgcaat tcaacaacct gtttttcgct caacccgcaa tacgccgcga ccaccgccaa
    36901 atccggtccg cctgcaccac cgtaaaccac cggaatttca ataaagcgag actcaggttc
    36961 cagggcctcg ctctcctccc accagcgttg caaacgctct atggcatcca gcgccagcga
    37021 ctcaggatta cgcaaaatca ccgtgatatt gttcatgccg ggaatggctt caaccacatt
    37081 cggcatatcc accagacgct gcgccagtcg ccagatccgt ttctggctag ccagcgtcac
    37141 cggcggttcc agttccagca ctaccgccgt ttcacctatc agataacaac gcgctcgttg
    37201 cactttcgat ccctctgatt attcgttatg caggattagg aatatcaata aaggtcacat
    37261 caagatcggt attttcattc agccactcgc tcaatgcgcg aataccaccg cgttcagtgg
    37321 catggtgacc tgcagcataa aaatgcaatc cctgctcgcg ggctgaatga atggtctgct
    37381 cagaaacttc gccagtaata aaagcatcca cgccaaaacg cgcggcgcta tcgataaaac
    37441 tttgcccgcc gcccgtgcac caggcgacgc gctggaccac ctcaggtccg gtatcgccac
    37501 accataacgg cttacgtccc agacgcgctt caatccagga ggctagttcc attcccggca
    37561 caggcatggt cagctctccc cacggcacca acggctcaat ttcgcccatg accgtgatcc
    37621 ccagtaacgc cgccagctgt gcgttattgc ccagctcagg atgtgcgtca agcggcaaat
    37681 gccagccata caggttgata tcattcgcca gcaacgtttt taaacggtta cgcttcattc
    37741 cgcgaatgac cggagactct cctttccaga agtagccatg atgcacaatg accgcatctg
    37801 cgcccagacg cactgcctca tcgagcaaag cctggctggc ggtgacaccg gtaacaattt
    37861 tttgcaccgt ctctttgcct tccacctgca aaccattcgg cgcgtaatca ctaatcgccg
    37921 cgctgttcag tttttcgtta atcagttgtt ccagttcggt gtttttcatc ttcgcgcctt
    37981 ccgtattctg atagctacac cgttaacata gcgttacatc gcctgttcgt cgatagccaa
    38041 tcttttgaca tattaagaaa caaacccgac gccaaaagac gcaaattgtt tatatcatca
    38101 cgcttcagct aatgtatatt tatgcaatca gacagcatat tgcataaata atcagatctg
    38161 tatcaaagtt ttaactttaa ggaaacacaa gcacgccgcg caactattac gtgacgttgc
    38221 cgtcaattgt ggaattatta tgaataaaca cgcatcacaa ccgcgcgcta tttactatgt
    38281 cgttgcgctg caaatctggg aatattttag cttttacggc atgcgtgccc tgctgattct
    38341 ctatctcacc aatcaactaa aatacaacga tactcacgcc tacgagttat ttagcgccta
    38401 ctgttcgctg gtgtatgtca cgccaatcct cggtggcttt ttggcggata aagttctcgg
    38461 caatcgcatg gcggtgatgc tgggggcgtt gttgatggcg atcggtcatg tggtgctggg
    38521 tgccagtgag atccatccgt cattcctcta tctgtccctg gcgattatcg tctgcggcta
    38581 tggcctgttt aaatctaacg taagctgtct gctcggcgag ctgtatgagc caaccgatcc
    38641 gcgtcgtgat ggcggtttct cgctgatgta tgcggcgggt aacgtggggt ctattatcgc
    38701 acctatcgcc tgtggttacg cccaggaaga gtacagctgg gcgatgggct ttggcctggc
    38761 ggcggttggc atgatcgcgg gtctggtcat tttcttatgt ggcaatcgtc atttcactca
    38821 tacccgcggc gttaacaaaa aagtactgcg tgcgacaaac tttctcctgc cgaactgggg
    38881 atggctgctg gttctgctgg tggcaacgcc tgcactgatt accgtgctgt tctggaaaga
    38941 gtggtcggta tacgccttaa ttgtcgcgac tattattggc ctgggtgtac tggcaaaaat
    39001 ttatcgcaaa gcagaaaacc agaaacagcg gaaggagctg gggctgattg tgacgctcac
    39061 cttcttcagt atgttgttct gggccttcgc acaacagggc ggtagctcga ttagccttta
    39121 tatcgaccgc ttcgttaacc gcgatatgtt tggttatacc gttccgaccg cgatgttcca
    39181 gtcgattaat gccttcgcag ttatgctgtg cggtgtgttc ctggcgtggg tggttaaaga
    39241 aagcgtcgca ggtaatcgta ccgtgcgcat ctggggaaaa tttgctcttg gccttggcct
    39301 gatgagcgcc ggattctgca ttctgacctt aagcgcccgc tggtccgcaa tgtatggtca
    39361 ctcttctctg ccactgatgg tgttaggcct cgcggtgatg ggctttgcgg aactgtttat
    39421 cgacccggtt gccatgtcgc aaattacgcg cattgaaatt ccgggcgtga ccggcgtatt
    39481 aaccggcatc tacatgctgc tttctggcgc gatcgcgaac tatctggcgg gcgtgattgc
    39541 cgatcagaca tcgcaggctt cgtttgatgc ttccggggcg atcaactact ccatcaatgc
    39601 atatattgaa gtatttgatc aaattacctg gggcgcactg gcgtgtgtag gactggtact
    39661 gatgatttgg ctgtatcagg cgctgaaatt cagaaaccgc gcgctggcgc tggagtcata
    39721 ataggccagc accgatgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacgtatttc
    39781 ctgcaattta ttgaatttgc acaaatttgt aggctggatt aggcgttcac gccgcatccg
    39841 gcatctgggc tcgattgcct gacgcgtctg ttatttcccc ttccgcgccg cctcatacgc
    39901 tgccaacgtt tgtactctcg cctctttgtg ctcgactatc ggttgcggat aatccagctt
    39961 cacacctgct ttctgcgccc acttccacgg ctcatgcacc gctttccctg gtacatcgcg
    40021 cagctccggt aaccatcgac gaataaactc gccctcacgg tcaaatttct cgccctgagt
    40081 tatcggattg aaaatacgaa aatacggcgc agcatcagta cccgtagaag ccgcccactg
    40141 ccagccaccg ttattagctg ccaaatcacc atcaatcagc tgcgacatga aatatcgctc
    40201 gccttcgcgc cagtcgatca acaaatcttt caccagaaaa ctggctgtaa tcatccgtag
    40261 ccggttatgc atccagccag tgctgttaag ctgacgcata gcggcatcaa taatcgggta
    40321 tcccgttttg cctttctgcc aggcctgtaa atgtgcggga ttgctttgcc actgcacacg
    40381 atccgtccag gcaataaatg gacgatgttt acacaacgag gggtaatacg tcatcagatg
    40441 acggtagaac tcgcgccaga tcagctcact aagccagaca ctaccggccc caccgtccag
    40501 cgcctgcggc tgttccgcca acaagcgatg caaacactgg cgaggcgata acccgcccgt
    40561 tgccaagctg gcggacaaac ggctggtgcc ttccactgcc ggaaaatctc gttgttgctc
    40621 atactctcca gcaccgttct cgcaaaactg gcgtaattgc gcaatcgccg ctttttcttc
    40681 caccgggaaa tgcgcagtat cgaaagactg acgaggataa ttcagcgtaa tggatggcgc
    40741 gggctttatc gatccgctac tacgaacttt tggtgcagcg acgcactccg gcatcccttc
    40801 ccgcagccgt ttcagccagg cattcttaaa aggcgtaaag actttgtaca tctcgtgatt
    40861 gccggtcatc accgcccccg gcggcaggat cacgctgtca tcaaatcctt cacacaccac
    40921 gttacgcagc gctctttcaa cgtccacatc ccgcgctcgc tcattcacct catactgata
    40981 gttataaaaa agatgagtaa cgctgttttc cgcgcacacc tgtttaacta tttcgacact
    41041 ggcgacaaag tcatccactt cacggaacaa taaaggaata cctttttccg ccagcgcgat
    41101 ttgtagccca ttcagttgag cattgataag ttcagcctga cgcggcgaca tgttatgcgt
    41161 ctcccactgg cgtggtgtag cgatatacag cgccagcacg cgtgcagacg aattgcggca
    41221 ggcggcagcc agtgcgagat tatcgtgcag acgtaaatcc tggcgaaacc aggccagatg
    41281 ggtagtcata aaactcctga atggcgcaag ttaatcacac gcggccaaag ttcgaaataa
    41341 cgctgccctg aaagccaggc gtcaggataa agcgccatat agtgtttgat acgcatcagc
    41401 ggcgcaagaa gtggttgctc gccacggcga taactgtcga taagcgcagc cagctgctgg
    41461 cgttctgtgg aatcaatatg cgggcgaaaa taaccctgaa cgtgcatcaa aacgttggtg
    41521 tgatcgcgcg ggttggcggg atgagaaagc aaaacaatta cgcgttggcg gtactggttg
    41581 taaaaatcat ccagattttg ccactgatga atatcagcca caaacgggcc aagtttgcgg
    41641 tactcgggct gagaatgggc cagaaagacg agtttatagc ggctgtgata atcgagaagc
    41701 gccccgcgcg ttaagccatg ttctttcagt cgatgtaact catgaagggc gaagatacgg
    41761 attagggtgc tgtcatcaaa tcgttgtaga ttcattactg ctcctgaacg gtatacagga
    41821 gtaagtgtag gcgttggtaa atgatgtttg tgaagttaat ccgctagcag ggcaagatta
    41881 cgaaagtgcg ctccccgcaa gcaactaatc aaggtagtta atcatggctg aacacgatat
    41941 tacgccagaa gtcaccattt ccaaaacaca gcgccgctat aaagtcggct atgtcagagc
    42001 ccgccaccag gaccgcaaaa ccggtatagc ccggtattat tcacaacatc ccagcctgca
    42061 ccttaagggc gactggctga aagaggccgg atttgatacc ggacggggtg tcacggtgaa
    42121 gatttcggag gggggcctga ccatcatcgc tgacagtgac gaggtgcagg aactgcggga
    42181 tgggatgttt agtgtgctga acgaaagtta aatattaaaa aagctgggaa gatttaaaac
    42241 cttcctagct ttttattctg tcttattata atggatactc atcatcttcc gatgaattta
    42301 taaattgctc caagtttaca tgaagagtac ttaacctagt ttttaactca ttaagtgaag
    42361 cgataatttc atattttcca acataatcat atgatggatc gtattcatta tctatgtcag
    42421 aagctaaaaa tgatgatatt cgtagtttaa atttagcttc atctttatta cattcattgg
    42481 cataaggtga atgccctatc atagcatatt catctaatct aaaaggataa gatggtttta
    42541 attcatcgat caatgaaatt tcaatccatg ttctatatcc aggcattaat ggactaattt
    42601 ctttgagcaa aacctttcca cttaataatg tattgagatt gttcattatt atgtctccca
    42661 cgggaaaaat atatgtctaa cacttccacc atgttgatga acttgtaaat gtggtaaatc
    42721 accatcagga tttcctggtc catgacctga cactctatat gaaggttttg ttacagaagg
    42781 atcttttata tcatcccaat gatatgaacc tgcttttgtt ttatttccag taagatattc
    42841 cattaagttc ttagtgctgg ggcctgactc acctgtcata tttttataat tatgacctaa
    42901 atatctatcc ataaacaatt gttcagcgtc acttctagac ctaacagtaa ctttggtttc
    42961 actactatct aataaacgat tcgctttttg gttactaaca gcacatggag ttaatccaag
    43021 tggatcagca tattttagag gattaggtgc ataactgtag agatttaaac cgcctctcaa
    43081 ccctatcggg tcagttacca tgaaccgccc gacatccgga tcatagtacc tgtgcagatt
    43141 gtagtgcagc cccgtttccc tgtcgaggta ctgtccctgg tagcgcaggt tctgctccac
    43201 cccgccgtgg tccttctcct gtatcgtgtt accccagacc tgataccccg tctcccacac
    43261 gattttcccg tcgctgtccg tcatctcttc cggcgcgccg ttcacatccg tgtgaaaata
    43321 cagtatccgg ttcgggccct ctttgcccgc cttatccacc cgcgccagcg gttcataact
    43381 cccctggtcc ctgtagatgt acaggctgcc tgttccctcc ggcctgctct cctgcgccag
    43441 ccgcattccg tcccataaaa attttgtccg gttgtacggc ttaccgtcag ggctgatgtg
    43501 ctgtttttcc gtccgcctcc ccagtgcatc gtacacatac cggtatgtcc cccgcgctgt
    43561 cgtcacttcc accatccggt gttccgcatc ataacggtaa ctctgcgttc cgctccggcc
    43621 ctccttttcc accgcacggc caaattcgtc ataacggtag tgcacaccac ggtaagacat
    43681 cagccggttg aacggcacca cgctgttttc tgccgtggcc ctttcccccg cgcgcctccc
    43741 cagcaggttt cccgccgcgt cgtactgcca gctcgccaga tacccctcat cctccgaacg
    43801 acacgccgtg atgcgccccg tggggtcgta gaagtaatcc gtttcccccc gccggctgtg
    43861 ccgcgtcttc agcagttcat cctcgccact gtagctgtac tgccggtccg tgaacgtctc
    43921 cggtaccacc ccgcgcatcc cgctgtatgt ctccctgcgc ttcagccgcc cgccggggtc
    43981 gtaaagctgc cgcgtgctca gtcgcccctg gcttctgagc acttcacggt gcaggtggtc
    44041 gcgcgtgaac tcgctgatga cctcccggtc cagatttatc tgctggagat ggccgctgcc
    44101 gtagtacaga tggcgctgcg ttctgccgtc cggaaaggtg gtggcagtcc ggttgcccag
    44161 tttatcgtac tggtgggtca ggatgccctg cgccgtctcc tcgctcagca tccgccccag
    44221 tctgtcgtag ctgaagcgga taacttccgg cgcgtcagtt tcgtgccgct ggcgacggat
    44281 ttccagtagc tggtctgccg catcatggac gtattccgta cggccatcgg ctgtctcttt
    44341 tgccgtgagc cggcccgccg catcacgcgc cagccggtgg cggatttcac cgccccggtc
    44401 cccggtgaac accacttccg tgaccgcgtt cagcgcgttg tagcggtacg actggcggca
    44461 gccgtccggg tttatctgct ccgccagacg gtccagcaca tcatggcgga accggtagcg
    44521 ttctccgttc tcgttcgtga gcgtgaccag acgcccgtaa gcgtcgtacc cgaatgccgt
    44581 cctgcgcccc atcgcgtccg tccggctgcg cacccggccc tgcccgtccc gctgccagcg
    44641 cgttatcctc cccatcgggc tggtgtactt taccagcctg cccgccgcgt ctgactggta
    44701 attcaccgtg cggccgtccg ccagtatcac ctccgtcaga tgaccgctgt cgtcataccc
    44761 gtagcgcgtg ctgtggctct gcgcatccgt cattctgata agcctcgtgc gttcgtcgta
    44821 gaaccacgcc gtctgcgagc cggaacagtc cgtgtgccgc atgagctgcc cgtcttcatt
    44881 ccactggagg tatttattcc ccccgcgcgc atccgttatc tgcaccacct ggccgtggtg
    44941 gtcatactga taccgggtgt tctgctgcag cgggtcggtc acggcaagaa ggttgccgcg
    45001 ttcatcgtac acacagcgcc aggtgtttcc cgccgcgtcc acctccgttt ccggctggtg
    45061 ccacaccggg tgccactgcg cctgctccac ccgccccagc gggtcgtgcg tctccgtgat
    45121 atgcccctgc aggtcataca catagcgcca tttgccgccc tgagggtcgg tcatccccag
    45181 caacagtcgt tcttcgtcgc tccagcggaa tgtcgtcacc tgtccggctt cgtcctggta
    45241 cgccgtcacc tgattctgcg catcccactg gtgataacgt gtgcgcccca gtccgtccgt
    45301 cacgcacagc ctgcgctgcg ccaggtccca cgccagccgg tactgttcac cgctgtccgt
    45361 gtggtgcccc gtcacccgcc atgccgcgtc atccggacct tcataccagc cccactggta
    45421 ataataccct tcgccgcccg gctggcggtg cagcgtcatg cagtggtttt cgtcgtaagc
    45481 aaactcacgc agcaggtgcc ccgtgctgtc gtacacgccg ttcaggtggc cggcctcgtc
    45541 atagctgtaa cgacgcagca gtaaaggtgc agtctcaggg aaatgctgat aaatctgtgt
    45601 cacccgccgg ggatgcgccg ccagttcgta atacagacgg atgcacggac gctgctgttc
    45661 gccgctgatt tccacaatgc ggccccggtc gtcgtagtac agattcagac agttgctgtt
    45721 acggtcgccc agcattttca gccgctggcg ctgcgggtgg tgcgggtcag cctcaaacag
    45781 aaaacactgc ccgtcatcgc tgctgataag ccagtgcccc tgctcaccac gccttaccgc
    45841 cagcccttca ccggggctgt aaaacccgcc ccccggcagt acggcttcca tatccaagcg
    45901 tcgccccatg cccgacacaa aggtcatgca gttttcgtct gcgctacccg gtgtgcacac
    45961 gaggctgact tcatacatta cactccagcc ctgaccaaac attccttccg tacgttcgtc
    46021 acgactgctg tacacgcgct gccagtccag cggaaagtgg cccggcagac tgaaatcgaa
    46081 gtcatcgtca tcggccaggt atttggcccc ggtggcggca ttgaacggat gcggaagggc
    46141 actggtggcc ttctgcgtca gcatgctcat cccgacactg gcagcaaaac aggcgatttt
    46201 agaaagcatt ttgcccgggc gaagaaacat cagcgccacg gttgccacca gggttatctg
    46261 cgacctgccg cttctgatat ccctgaccac cagtaacgga ccgccaatgc gcacatccgg
    46321 tgacacaaac gccccgttcc ccggctcatt caccaccctg gcctcgcagg tacagcgtgc
    46381 cccgctgcgt gtcgccggct ggctgttgat acgcacatcc cgcgcgcctt ccgcgatata
    46441 ttccgctgcg ggcggctcgt catcgccttt gaacagatta ctgatgctgc caataaccgc
    46501 attcactccc tgaaagacgg tttccagcat accgcccgga ttcagcaggt taagggccgt
    46561 tcccaggggg gcatccagaa aggcggcttt ttt
//
LOCUS       DAJVPP010000020        45786 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVPP010000020 DAJVPP010000000
VERSION     DAJVPP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.020"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1652
                     /locus_tag="OQM34_001111"
     CDS             <1..1652
                     /locus_tag="OQM34_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCS1451643.1"
                     /translation="DYAALSRAGMNFYDRRCQLEDQLEYQTLKTRAEAGAKRLLSLNL
                     KKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSWSAKLQGLLASCQP
                     AAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPVPNDIAYLQYTSGS
                     TRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMGLVGFLLPPVATQL
                     SVDYLRTQDFAMSPLQWLKLISKNRGTVSVAPPFGYELCQRRVNEKDLAELDLSCWRV
                     AGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSFSDEASGVVVNEVD
                     RDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPVAERVVGHICISGP
                     SLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLIIIRGRNIWPQDIE
                     YIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHALAARIQSEFGVTAA
                     IDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            1649..2563
                     /locus_tag="OQM34_001112"
     CDS             1649..2563
                     /locus_tag="OQM34_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCS1451644.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2596..2844
                     /locus_tag="OQM34_001113"
     CDS             2596..2844
                     /locus_tag="OQM34_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1451645.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2844..4015
                     /locus_tag="OQM34_001114"
                     /pseudo
     CDS             2844..4015
                     /locus_tag="OQM34_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(4051..5130)
                     /gene="lptG"
                     /locus_tag="OQM34_001115"
     CDS             complement(4051..5130)
                     /gene="lptG"
                     /locus_tag="OQM34_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1451646.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5127..6197)
                     /gene="lptF"
                     /locus_tag="OQM34_001116"
     CDS             complement(5127..6197)
                     /gene="lptF"
                     /locus_tag="OQM34_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1451647.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(6228..6788)
                     /locus_tag="OQM34_001117"
     CDS             complement(6228..6788)
                     /locus_tag="OQM34_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1451648.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6800..7624)
                     /locus_tag="OQM34_001118"
     CDS             complement(6800..7624)
                     /locus_tag="OQM34_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451649.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(7624..8973)
                     /locus_tag="OQM34_001119"
     CDS             complement(7624..8973)
                     /locus_tag="OQM34_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS1451650.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9019..9777)
                     /locus_tag="OQM34_001120"
     CDS             complement(9019..9777)
                     /locus_tag="OQM34_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451651.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(9809..10522)
                     /locus_tag="OQM34_001121"
     CDS             complement(9809..10522)
                     /locus_tag="OQM34_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1451652.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            10696..11388
                     /locus_tag="OQM34_001122"
     CDS             10696..11388
                     /locus_tag="OQM34_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1451653.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11437..12936)
                     /gene="pitB"
                     /locus_tag="OQM34_001123"
     CDS             complement(11437..12936)
                     /gene="pitB"
                     /locus_tag="OQM34_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCS1451654.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13228..15087)
                     /gene="gss"
                     /locus_tag="OQM34_001124"
     CDS             complement(13228..15087)
                     /gene="gss"
                     /locus_tag="OQM34_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1451655.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15292..16158
                     /gene="yghU"
                     /locus_tag="OQM34_001125"
     CDS             15292..16158
                     /gene="yghU"
                     /locus_tag="OQM34_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1451656.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16281..16529)
                     /gene="hybG"
                     /locus_tag="OQM34_001126"
     CDS             complement(16281..16529)
                     /gene="hybG"
                     /locus_tag="OQM34_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1451657.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16663..17004)
                     /gene="hypA"
                     /locus_tag="OQM34_001127"
     CDS             complement(16663..17004)
                     /gene="hypA"
                     /locus_tag="OQM34_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1451658.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(16997..17485)
                     /gene="hybE"
                     /locus_tag="OQM34_001128"
     CDS             complement(16997..17485)
                     /gene="hybE"
                     /locus_tag="OQM34_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1451659.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(17478..17972)
                     /locus_tag="OQM34_001129"
     CDS             complement(17478..17972)
                     /locus_tag="OQM34_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1451660.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(17972..19675)
                     /gene="hybC"
                     /locus_tag="OQM34_001130"
     CDS             complement(17972..19675)
                     /gene="hybC"
                     /locus_tag="OQM34_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1451661.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19672..20850)
                     /gene="hybB"
                     /locus_tag="OQM34_001131"
     CDS             complement(19672..20850)
                     /gene="hybB"
                     /locus_tag="OQM34_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1451662.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWSLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20840..21826)
                     /gene="hybA"
                     /locus_tag="OQM34_001132"
     CDS             complement(20840..21826)
                     /gene="hybA"
                     /locus_tag="OQM34_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1451663.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21829..22947)
                     /gene="hybO"
                     /locus_tag="OQM34_001133"
     CDS             complement(21829..22947)
                     /gene="hybO"
                     /locus_tag="OQM34_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1451664.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23136..23423)
                     /locus_tag="OQM34_001134"
     CDS             complement(23136..23423)
                     /locus_tag="OQM34_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1451665.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(23542..24429)
                     /locus_tag="OQM34_001135"
     CDS             complement(23542..24429)
                     /locus_tag="OQM34_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1451666.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24586..25626
                     /gene="gpr"
                     /locus_tag="OQM34_001136"
     CDS             24586..25626
                     /gene="gpr"
                     /locus_tag="OQM34_001136"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1451667.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25666..26160)
                     /locus_tag="OQM34_001137"
     CDS             complement(25666..26160)
                     /locus_tag="OQM34_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1451668.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVLDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26351..27235
                     /gene="yghA"
                     /locus_tag="OQM34_001138"
     CDS             26351..27235
                     /gene="yghA"
                     /locus_tag="OQM34_001138"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1451669.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISSGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27507..27932)
                     /gene="exbD"
                     /locus_tag="OQM34_001139"
     CDS             complement(27507..27932)
                     /gene="exbD"
                     /locus_tag="OQM34_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1451670.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(27939..28673)
                     /gene="exbB"
                     /locus_tag="OQM34_001140"
     CDS             complement(27939..28673)
                     /gene="exbB"
                     /locus_tag="OQM34_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1451671.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            28925..30112
                     /gene="metC"
                     /locus_tag="OQM34_001141"
     CDS             28925..30112
                     /gene="metC"
                     /locus_tag="OQM34_001141"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1451672.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30252..30911
                     /locus_tag="OQM34_001142"
     CDS             30252..30911
                     /locus_tag="OQM34_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1451673.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(30951..31850)
                     /gene="yqhC"
                     /locus_tag="OQM34_001143"
     CDS             complement(30951..31850)
                     /gene="yqhC"
                     /locus_tag="OQM34_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1451674.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32044..33207
                     /gene="yqhD"
                     /locus_tag="OQM34_001144"
     CDS             32044..33207
                     /gene="yqhD"
                     /locus_tag="OQM34_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1451675.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33312..34139
                     /gene="dkgA"
                     /locus_tag="OQM34_001145"
     CDS             33312..34139
                     /gene="dkgA"
                     /locus_tag="OQM34_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1451676.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            34339..35265
                     /locus_tag="OQM34_001146"
     CDS             34339..35265
                     /locus_tag="OQM34_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1451677.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35316..35573
                     /gene="yqhH"
                     /locus_tag="OQM34_001147"
     CDS             35316..35573
                     /gene="yqhH"
                     /locus_tag="OQM34_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1451678.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35616..37835)
                     /locus_tag="OQM34_001148"
     CDS             complement(35616..37835)
                     /locus_tag="OQM34_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS1451679.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(37946..39358)
                     /gene="ftsP"
                     /locus_tag="OQM34_001149"
     CDS             complement(37946..39358)
                     /gene="ftsP"
                     /locus_tag="OQM34_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1451680.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKDDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39433..40170)
                     /gene="plsC"
                     /locus_tag="OQM34_001150"
                     /gene_synonym="parF"
     CDS             complement(39433..40170)
                     /gene="plsC"
                     /locus_tag="OQM34_001150"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1451681.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40404..42662)
                     /gene="parC"
                     /locus_tag="OQM34_001151"
     CDS             complement(40404..42662)
                     /gene="parC"
                     /locus_tag="OQM34_001151"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1451682.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(43208..43690)
                     /locus_tag="OQM34_001152"
     CDS             complement(43208..43690)
                     /locus_tag="OQM34_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCS1451683.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(43743..44135)
                     /gene="ygiW"
                     /locus_tag="OQM34_001153"
     CDS             complement(43743..44135)
                     /gene="ygiW"
                     /locus_tag="OQM34_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1451684.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            44287..44946
                     /gene="qseB"
                     /locus_tag="OQM34_001154"
     CDS             44287..44946
                     /gene="qseB"
                     /locus_tag="OQM34_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1451685.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            44943..>45786
                     /gene="qseC"
                     /locus_tag="OQM34_001155"
     CDS             44943..>45786
                     /gene="qseC"
                     /locus_tag="OQM34_001155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1451686.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMIRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHS"
BASE COUNT        11364 a        12156 c        11468 g        10798 t
ORIGIN      
        1 tggactacgc cgctctgagt agagccggaa tgaattttta tgacagacgt tgccaacttg
       61 aagatcaact ggaatatcag acattaaaaa cgcgtgccga agctggtgcg aagcggttgt
      121 tatcgctgaa cctgaaaaaa ggcgaacgcg tggcgctgat tgccgaaacg agtagcgggt
      181 ttgtagaggc tttttttgcc tgtcagtatg ctggcttagt cgccgtcccg ttggcgattc
      241 caatgggcgt cggtcagcgg gattcctgga gcgccaaact ccagggttta ctggcaagtt
      301 gccagcccgc agccattatc actggtgatg agtggttgcc actggtcaat gccgcgacgc
      361 ataacaaccc cgaattacat gttttaagcc acgcttggtt taaggcatta ccggaagccg
      421 atgttgcgct ccagcgtcca gttccgaacg atatcgccta cctccagtac acctccggca
      481 gcacccgttt tccccgtggc gtcattatca cccatcgcga agtgatggct aatctacgtg
      541 ctataagcca cgatggcatt aaattacgcc ctagcgaccg ctgcgtctcc tggcagcctt
      601 tctaccatga tatgggactg gtcggctttc tcctgccccc cgtcgccacg caactttcag
      661 tagattattt gcgcactcag gattttgcca tgagtccttt gcaatggctt aaattgatca
      721 gtaaaaatcg cggcaccgtt tccgttgcgc cgccgtttgg ctatgaattg tgccaacgcc
      781 gcgtgaatga aaaagatctc gctgaactgg atctttcctg ctggcgcgtc gctggtattg
      841 gtgctgagcc gatctccgca gaacaactcc atcaattcgc tgaatgtttc cgtcaggtta
      901 actttgacaa taaaactttc atgccgtgct acggactggc agaaaatgcg ctggctgtca
      961 gcttctctga tgaagcctcc ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat
     1021 atcagggtaa agccgtcgcg ccgggtgcag agacacgcgc cgtatcgact ttcgtcaact
     1081 gcggcaaagc gttgccggaa catggtattg aaatccgcaa tgaagcaggt atgccggtcg
     1141 cggaacgtgt ggtaggccat atttgcatct ccggtcccag tctgatgagc ggttactttg
     1201 gcgaccaggt ttcgcaagac gagattgccg cgacgggctg gttagacacc ggcgacctcg
     1261 gttatctgct ggacggttat ctgtatgtca ccggacgcat taaagatctg attattattc
     1321 gtggccgtaa tatctggccg caggatattg aatatatagc ggaacaggaa ccggaaattc
     1381 attctggcga tgcgattgct tttgttaccg cccaggaaaa aatcattttg cagatccagt
     1441 gtcggatcag cgacgaagaa cgtcgcgggc agcttatcca cgcgctggcg gcacggatcc
     1501 aaagcgaatt tggcgtgacc gcggctatcg atctgttacc gccccacagt attccccgaa
     1561 cgtcctccgg caagcctgcc cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg
     1621 ccagtcttaa tgtgcaggaa tccctggcat aaatcaaact gtcgcggtga cgggcgctac
     1681 cgggtttatc ggtaaatata ttatcgataa cctgctcgcc cgcggctttc acgttcgcgc
     1741 attgacgcgt actgcccgcg ctcacgtcaa tgataatctc acctgggtgc gcggttcgct
     1801 ggaagatact cattcactta gcgagctggt tgccggagcc agcgtagtgg tccattgcgc
     1861 cgggcaagtg cgcgggcaca aagaagagat tttcacccgc tgtaacgttg acggcagcct
     1921 gcgcctgatg caagcagcaa aagagagcgg cttttgccaa cgttttctgt ttatctcttc
     1981 gctggcggcg cgccatcccg agctctcctg gtatgcaaaa tccaaatacg tcgccgagca
     2041 acgactggca gcaatggcgg acgaaattac gctgggcgtt tttcgcccga cagccgtgta
     2101 tggtcccggc gataaagagt taaaaccgct gtttgactgg atgctacgcg gcctgctgcc
     2161 acgacttggt gcgccagata cacaactctc ttttcttcac gtcaccgatt ttgcgcaagc
     2221 agtgggtcag tggttaagcg ccgaaactgt acagacgcaa acctatgaat tatgcgatgg
     2281 cgtcgccggt ggctatgact ggcaacgcgt acgacagctt gccgccaaag cccgttgtgg
     2341 ttccgtgcga atggttgaca ttcctctgcc ggtactcaca ggccttgcgg atatcagtac
     2401 cgcgttgagt cgcctggcag gtaaagaacc tatgctgacc cgctcgaaaa ttcgtgaatt
     2461 aacccacgcc gactggtcgg caagtaataa ccgtatttct gaagatatca attggtttcc
     2521 cgggattagc ctggaacacg cattacgcaa cgggctattt tgattgtctt gttttaaagg
     2581 tgtgttaacg tttctatggt aaatcgtgaa atagtaatgg attacatcct ttcctgttta
     2641 caggatttgg tcgaaaacgg tgtggaaatt aagcccgaca gcgatctcgt caacgatctt
     2701 ggcctggagt cgatcaaagt gatggatctc ttaatgatgc tggaagacag attcgatatt
     2761 tccattccta ttaatattct gctggatgtc aaaacccctg cgcaattaat ggaaactcta
     2821 cttccatggc tggagaataa ataatggggc tatacgataa atatgcgcgc cttgctggcg
     2881 agcgtctgca atttagtgac aacgggttaa caccgtttgg cacctgtatt gatgaagtgt
     2941 attccgccac cgaaggccgg atcggcaata aaaaagtgat tctggccggg accaataatt
     3001 atctcggttt aacctttaat catgatgcca tcagcgaagg tcaggccgcg ttagcggccc
     3061 aaggcaccgg caccaccggt tctcgtatgg caaacggcag ttatgcgccg cacctggcgt
     3121 tagagaaaga gattgccgag tttttcaatc tccctaccgc tatcgttttt caaccggtta
     3181 taccgctaat ttaggcgtca tcagcgcact tgccgaccat aacgccgtgg tgttactgga
     3241 tgccgacagc cacgccagta tttatgacgc ctgttctctc ggtggcgcgg aaattattcg
     3301 tttccgccat aacgatgcta aagatctgga acggcgcatg gttcgcctcg gtgaacgggc
     3361 gaaagaggcg attatcatcg ttgagggcat ctacagcatg ctcggtgacg ttgcgccgct
     3421 ggcggaaatt gttgatatca aacgtcgtct cggtggttat ttgattgtcg atgaagcgca
     3481 ttcgtttggc gtgttaggcg caacggggcg cggactggct gaagctgtcg gcgttgaaga
     3541 tgatgtggat attatcgttg gtactttcag taaaagtctg gcctcgattg gcggttttgc
     3601 tgtcggttcg gaagcgatgg aagtgctgcg ctacggcagc cgcccgtata tttttaccgc
     3661 ctctccttct ccgtcctgca ttgcgaccgt gcgctcgtcg ttaagaacta ttgccacaca
     3721 accagagctg cggcaaaagc tgatggataa cgccaaccat ctgtatgacg gtctgcaaaa
     3781 actgggttat gagttaagtt cacatattag cccggtagtg ccagtaataa tcggttcgaa
     3841 agaggaaggt ttacgcatct ggcgcgagct tatctcgccg ggcgtgtatg tgaatcttat
     3901 cctgccgcct gccgcgcctg caggcatcac gctgctgcgc tgtagcgtaa atgctgccca
     3961 cagtcgtgag caaatcgatg ccatcattca ggcatttgct actctgaagc agtaatgtga
     4021 gcccgccgtg gtgtttgcgc cacggcgggt ttaatgttgt ctgtcatacc aatacaccag
     4081 gctcaacgcc accaacacaa acgctaccgg taaaccgagc gcggtaacag gtgcgcttaa
     4141 cgcaaatagc aatcccagat taaccatgat ttgatagctg atccaggtga gtaagcccac
     4201 gatgacgcct acagcgagac ggctacccat cccaggcgaa cgcggggcac taaaggtaaa
     4261 cggcacagcc agcaaaatca tcgccagggt caggatgggt tgccccagtt tttcccacag
     4321 tgcaaggcgg tattcgctgc tgggttgccc ggtttcctgc aaataatgga tgtaatgatt
     4381 aagctgacgg acagaaaaag tattccccgg cattgataac tcttcaagat tcatactggt
     4441 gaaggccgat tgccaggcaa gattatctaa tgtttccagc gtttctttgc cattcagcca
     4501 ttttttatga ttcacaccat gcaggaccca cgttttgtcg tcttcaatgg tggcactgcg
     4561 tgcataaatg taggattcaa gcgagagatc gtcgcgataa tgaaatatct ccacgcccac
     4621 cggctggccc tgctcgttca gcgatttcac cgtcacaaat tcattaccac gcctggccca
     4681 cagcatattg ccggtaatgt catcgtcctc ccccaacgcg gtggcggtcg atttgatttg
     4741 caacgcctgt tgctgcaatg gcgacgccac ccactcatct atcgcgccta acgaaacagt
     4801 ccacaatatc ccggcaacca gcgccaccag tgcaatacgg aagatagaaa accccgtgct
     4861 gcgaatggcg gtaagctcac tgtttttcga taactgcccc agtccaacaa taccacccaa
     4921 taacgcgata aacggcgaaa gttcgaccag tgtgcgcggt aaggtcatta gcaccaccag
     4981 caccgcctga gtccagcgat aaccgcccgg gctgacacca tccagttcgt taatcaggtt
     5041 gaaggtggta aaaagcggca gcaatagccc tgcagcggcg gcaaaaccga gaaagagatg
     5101 acggattaaa tagcgactga aaacattcat cgggaaaatt tctgcagcaa agagaagtca
     5161 cgtgcgacca gtatcagcag gcccattagc atgagtccgg gcactaacca caaaccagga
     5221 atgaggggaa tcgcgccgtt agccaccagt gtacggcaga cgtcgccgcc ataaaaaatg
     5281 gcaataaaca gcaacgtcag tggcaataac gttgaaaatc gcccttgccg cggttttacc
     5341 cgacttaatg aaatggccag cagcgccatc aacaatgcgg tcaggccacg gctttgtcgc
     5401 cattgcagtt cggcatgatc ggcaggaaag gcggagcgcg ccagctccgt gaccgatttc
     5461 gctttgcgtt tgacgttcgg gctttgatcc agcggattca gatgtaattg cagattacgg
     5521 tagatctgtt cgttgtcgtc acgcccctga tgatcgagaa gataggcagt gccgttgtgc
     5581 aacataacgg tcggtttctc gggtgatggg tcaaccacat caaccgaacc ggcgcggaaa
     5641 atacgggttc gattggcagt agaagtataa atcagcgcgt cagtcaggcg attattatcc
     5701 tgatcaaccg tctgcgaaag gatcattcgt ccgttatcgt tagtgttaaa tttctttgcc
     5761 cgcaactggc gaacatccag ctccgactgc gactgctgct ccagttgata aatctgcgca
     5821 taagcccacg gacgaccata catcgacaga agggtgacaa aaatacttag cggtatcgcc
     5881 agataaagaa cggctttgta caagcgtccc ggactgcccc ccgccgcaga gatagcggta
     5941 atttccgagt cggtatacat ctgccctagc gtcacgccaa ctgacacata cagaccaaca
     6001 ggtaacaaca tctccagtgc aatcagtact ttgtagaaaa cgatatccag cacaacatca
     6061 agagctaatg tcccatttgc cgcttcggtc agataacgct gtgcggagta gctggcgaaa
     6121 ataaagatca gaaaaccgac gataatcagc accagacggc gcgtaccacg catgatgtag
     6181 tgttcaacca gcttcatctc actaacgccc tcaatccttt gagtttttta ttcattgatc
     6241 agtttctgcg ccaacgcaat gacatgatct acctcttccg gcgtcaggct gccgtccttt
     6301 gcccactgaa tttgcccggc tttattcagc accagaatgg ttgaactctg ctccggcaag
     6361 cgccaggcaa cgcgtccctg tccattgccg tcaataacga attgcgccca cggatagcgg
     6421 cgtttatttt tttcgatttt gccaacaaca aaatagccag taccaaagat ggcatcatcg
     6481 gtgttcacaa tggtggtggg ctgaaaacga tcctgcggaa agtttgccgc ttcaaccgct
     6541 ttaattaaca gtgagttctt tttcttcgcc gacttacgcc cggcgatata ctgaataatg
     6601 cggacttttc ccgctaattg tgtgctgttc cacttttggt agtgaatttc gtcgtcgcta
     6661 ttcagaagca gctcgccgcc ttcctggata taaacagggg taaccgtttt gccagtgacg
     6721 aaattgtgtg cctgcacacc cggggctaag agcatgataa tcaacgcaat aatcaaacga
     6781 gatgacatca ttgctccgcc tatagcggaa aacgcgaaat cagcagctct aagcgctgac
     6841 aaatatcgct atccagcgcc aacggtgcat aatacgactg gcggttttcc atatcatccg
     6901 ccaccatgtc gtcgtgttta tctcccatca ggccattaat ggcggcgagg aaacgggaaa
     6961 tctgtaacgc ctctttattt tcaggatcaa gcgatcttaa ggtgtcgtaa taagaaacct
     7021 gctggctgat gatttttcgc acatcatgaa attccctgcc ggttagtcgc ggtttggcaa
     7081 taatctcttt cagggtatgc atttgctttt gccgataggc aataaaactg cttacgcttt
     7141 ctaactggct atggcgtaat gaatagttca ccaacgacca gaccggagag cttaaataaa
     7201 tccgtaacag gttgccgtaa tacgacacaa tatttttctt accgttacga aagccgtcct
     7261 gaaaacgccc cagaaaaacg gtcgttttgc cgaataaaaa tccggcctga tgcttcttta
     7321 aatacagccg ttgcgcaaaa cgcaggtgct tgtagcgcgc tcgcatgtat ttaaattgtt
     7381 tgcgctcatc agcagtcagc tcatcgcctt tggcttgttt gcgaactaaa cgcagtaatt
     7441 cctgacgatc aacgccattg agccagaatt gcaggcataa cgcgtaatta tcatgaatga
     7501 tttcggtggt acaacttagt gcaatagtcg gcggcaaact aacctgtgca tcaacgacat
     7561 catcttcttc aatcgcaaca aatagacgct cgctcgcctc aagcgggaaa tattttctca
     7621 tcatcaggat ttctctccgg ctgcgttata aagcaaggaa tggcgctgaa aaaatgcctt
     7681 taaggttgga attaacgaga gcgccacgtc cagacattgc ccaccgaagt aagcaaacgc
     7741 tgctcccagc acaccaaaca gatgacagaa catgaacagc aatacgatgt agccgattga
     7801 ggcgatggtt tgcgccacga gaaaagcgcg ctgttttccc gccatcaata gcaatgactc
     7861 ctgcgggaag cccagcatcg aaatcacaat tgcgcccagc atcacctgaa tcaggtcata
     7921 cgcttcgaga tacttaacgc caaacaccag cgaaatgagc ggcttgccga cgatcaacac
     7981 cgcgagcgcc accagaatac cgattccgcc cgccagtaaa ccggacttca cacctaacaa
     8041 ccacggtctg gtggtgcgcg gatctaaacg catcacctct gggtaaaaac ttttacccag
     8101 caaacccgct ggcgttccgg cggcgtcgaa gaatgtcatg gcgattttaa ataacccggc
     8161 ggcagcgggt cctaacacga tccccaccaa cactgtgctg cacgagttac gcgccgacca
     8221 gatggagtgg gcaatgttgg ttgaccagac aaaactccac gcgcctttaa tgtgtcgggc
     8281 agactcaaac agattcaatt tgaaggcgtt atggatattt ctacggcgta attcgcgcgc
     8341 ggcaaaccac cagtacatgg tgccgccaac cagattcgac acgtaccagg caataacaaa
     8401 acccgcaaaa ccaaagtcaa aataccaggc tgcgacgctc cccgctgcgc gcagaaaagg
     8461 tttcgtcgcc tgctgtacag caatcaaatc aaagcgatct accgcacgca gaatgccggt
     8521 cggcgtggag gaagccattg aaggaatgag cgtgcaatac agcgccgcca gccaaaagct
     8581 ttggtcatcc agacctaatg aatgggaaag gaatggcagt aaggcaatgc cgccgacaat
     8641 cgccaccgcg ccgctgacga tatccagcga gaaggaaaat gagacgacat tgcgaaattg
     8701 ctgcggatta ttgttggtca aggctggcgt tccgtactga accaccagct gccatgtctg
     8761 aaacttaata aaatcgctga tcgacttggc gtacgattgc acaatcacca gtacaccaaa
     8821 catggcgggc gtcatccctt taccggcaca cgagagcgcc aacagcccca gcaaggcgct
     8881 ctcgacatta ctggagccta accaggcgct attgcgaata atggtgcgaa acgcgccatc
     8941 tgcaaaccaa tgtttgatgt taaaacccgc caattcagcc tgaccttatc gttgatagta
     9001 aaaaatatcc cgccagcctt aagttaaact tcggcggtca gaaacgatgg caaccagaga
     9061 aaccgccttc tgtacctgtt ccagcacttc gctgtagggc gctctggaat caatctcaag
     9121 aatttttgtg ccgttatagc caatcgtcga catgacgccg attttgtcct gcagctcggc
     9181 atactcatgg tcaggcttgc gggagatggc agtatcaata tcaatcccca aacgaatgat
     9241 taattcagga cggtattgcg ccattttttg gtataaactc cgttcgcgct gcgccagaaa
     9301 catgctgatt ttcccggtcg cacgttcgac gccgatcccc ggtccgtcat aataaaagcc
     9361 cgaaatttca gcctgcggga agcgatcgct gaccaccaga acgccacttt gcgccagtcg
     9421 ctgaaccttg cgtagattcg ccattcttcg cagcgagaag cagtacataa tcaccgccgc
     9481 ccatagcgcc ggagatttgg ttttcatgct ttgggttttc gaggatttgg ccgccaatcg
     9541 ccgttccagc cagacgccaa ccaacggcaa tcgtttgatt ttgtcgccgt cttcgccgga
     9601 gagcagcccc agatagcggc gctcggtttg ccagtgttgt tgcagcgatt tcaccaggtc
     9661 ggtggtcagc gtggatttac cggtgccatc acacccgacc accgcaatca gccccggaat
     9721 gtagttgggc tgcggcgcgg ttgtactatt aacagtttgt gtttgtagtg catccattcg
     9781 ttaaatcatc ccctatccat tttggtgatc aggaaagcgc ggcgtgaatt gcgcgtagtg
     9841 acgcttgcat aatttcatcg gctggttgcc gaccatccag ttcaaggatc ttggcgccat
     9901 tgaatgtcag ttgcggcgta acggcgattt tttcctgtaa cgctgccagt tggtggtcag
     9961 gtttacgcgc aaacgcggtt tgttcatcaa tgccaagacg aatcaacaat acgggtaaat
    10021 aagacgccat ccattggtac agcttcaact cgcgctgcct taacattttt atccaaccgt
    10081 taccgcccgt ggtttttgcc aattgcgggc catcaaagcg aaaacccggc acttcaacct
    10141 gcgggtagcg gtcggtgatg agcagatagc cttgctggct tttacacaac attttgcgaa
    10201 acttgtacgc ccgccagcag gaaagcagat agataaccag cgcagtaata ttgccaggcg
    10261 gagttaaggg cttttcgtgt acatgtgccg ctttacttcg cagataacac ccacaaggtg
    10321 cgccaataac agggagctgt gaaatccatt cgccaattcg cccggacgat tgcccgagat
    10381 aaatgtgttc tgttggcatt cttgctgcca gttcatttac caggcttgcc gtgagggtcg
    10441 atttacctga accatcacat cctacaatgg caataacacg cactggcgtg gaattaatta
    10501 ttgacatatt acgttgattc acgaaaaacc cggcagtaaa taatgtattg aatattacgt
    10561 ggtccgtgct cagaatatcc gttcaacctt attgtgacaa tggctaatta tgacattgac
    10621 ctgaattacg ctttcataaa aacatattaa ccaaataaat atttttaata gatatttaaa
    10681 ttaaaggata tattcatgca gtcaataaca cctccattaa ttgccgttat tggtagcgat
    10741 ggttcaggca agtcaacggt gtgtgaacat cttattaccg ttgtcgaaaa atatggtgct
    10801 gccgaaagag ttcatttagg aaaacaggcc ggaaatgtcg gtcgtgcagt gacaaaatta
    10861 cctttgatgg gaaaatcctt acataaaaca attgaacgaa atcaggtgaa aacagcaaaa
    10921 aaattgcctg gaccagttcc ggcgctggta attacagcgt ttgtcgcccg tcgcttactg
    10981 cgctttcgtc atatgcttgc ctgtcgtcgt cgcgggttaa ttgttctaac cgaccgttat
    11041 cctcaggacc aaattcctgg cgcttacgat ggtacggtgt tcccacctaa cgttgaaggt
    11101 ggtcgttttg tctcatggct ggcaagccag gaacgtaaag cgtttcactg gatggcgagc
    11161 cataagcctg atctggtcat caaactcaat gttgaccttg aagttgcctg tgcacgtaaa
    11221 cccgaccata aacgggaatc gctggcgagg aagattgcca taacgccaca gttaaccttt
    11281 ggtggtgcac aactggttga tatcgatgcc aatcagccac tggaacaggt gttggttgat
    11341 gcagaaaaag cgattacgga ttttatgacc gcgcgtggtt atcactagtc aaaaatggaa
    11401 atgcccgatc gccaggaccg ggcattttca ggaaggttaa atcaactgca atgctatcca
    11461 gtacagccca ccagaaagaa aaattgccgc cggtaaagta aacacccacg ccatcaggat
    11521 gctggttacc gttttacgct gtaacccacc gccgtccacc accatcgtcc ctgctaccgc
    11581 agacgagagg acgtgtgttg tggagacagg catcccaata taactggcaa ggccgataga
    11641 cactgccgcc gtcatttgtg ccgccatgcc ttgcgcatac gtcatgccgc gcttaccaat
    11701 cttctcaccg atggtcatcg ctacacgacg ccagccaatc atggtgccaa tgccgagcgc
    11761 cagtgctacc gccatgatga tccacaccgg agcgtactca atggtgctta acatatcgct
    11821 gcgcagtttt ttcagcaggt tctggtcttc tttactgacg cctggcagtt tcgccagctt
    11881 cgcggaggta tcggagatgc acagcataat gcggcgcagc tggctgcgct gactcacgct
    11941 taacggctcg taactttcca tattgcctgg cagcatcgtt ttaacgcgcg caatcgcatc
    12001 aaaggtattt gccggatgac agtgaaactc tgttacttgc gtgccatcag tcgatgctgc
    12061 aggcaatgga ggttccatcg caatcaactt ctgcggcagt tcaggatgct gttgcaggta
    12121 gtgttcgaag ttggtaacgg catcgcgggt acgggtaatt tcatagccgg acgcattcat
    12181 attgacgacg aagccagcag gggcaatccc caccagtacc agcattacca ggccgatccc
    12241 tttctgcccg tcgttcgcgc cgtgcgaaaa cgccacgccc gcagcggaaa caatcagcgc
    12301 aatacgcgtc cagaatggcg gtttacgttt gccctttttc tttttgcgat cttccggaat
    12361 gcggtgaata cggtcacgct ttttcgtccc actccagaaa cggcgcagca ggaaaatcag
    12421 accacccgca atgaccaggc cgacgatagg ggaaacaatc agcgaggaga aaattttggt
    12481 tacttcacgc aggtttaacg catccatcac cgatgagccg gttaacagcg cgttggttaa
    12541 accgatgccg ataatcgcac caatcaaggt gtgcgaactg gaggccggta aaccaaagaa
    12601 ccacgttccc aggttccaga taatcgccgc cagcagcatg gaaaagacca tcgccaaacc
    12661 gtgagttgac cccatattca gcaacaaatc gattggtaac atatggacaa tggcataggc
    12721 aacgctaagt ccgcccaata acacgccaaa aaagttaaaa aatgccgcca tcaccacagc
    12781 aagttgtggc tgcatggcac gagtataaat aacggttgcc accgcattcg ccgtgtcatg
    12841 aaaaccattg attgcttcgt agaacaacac aaatgccaga gcaagtaata acaaaagccc
    12901 cgtatatata tcaaggccaa caaataaatt tagcatatat tagattacgc cattttgaat
    12961 ttacgaacgg acgcattatc agtgacttta acggcatggg caaagtgaaa tatcattttt
    13021 taacgataaa aagagtgtta tttcttgtgt atcagacaat aagcattcat tcaaaacctt
    13081 tagaaataat caaaacacag gtattccact ggttaatata ttcacatatg aaatgaataa
    13141 atattggaat atataaatat tgaatatttt gattaatccc cgtactaatt attcttcata
    13201 atcagtacgg ggttgctaca acatgtatta ctttttcacc acaattaacg gttcaatatc
    13261 actctctttt ttgatgacca gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc
    13321 gccaacggtg aaggtacata cctgaatgta tttaccgtcc actttcggca aacaccacag
    13381 ttgctgatag atgtttttct gctcggcaaa tttaccgctg gttttgtcca gcacctcttc
    13441 atgatggctg accaggtcga tattgctacc acagcgaccg gcgatcggtt tcactgcgta
    13501 acctgttttc accaattcat cattaacagt gaaatcggta tccagcaggt aacgatggtg
    13561 cgggaacagc gaccagagga tcggcagaat cgctttgttg ccggggatca ccgtccacag
    13621 cggctcaaag accagcactt ccgggcgcag caatacgtcg ataagacgca cttcgttttg
    13681 cggatgaccg gtacggattg gcaccgcagc aaactcacgg tcgctaactt cacgaatctg
    13741 atcaaacgcg gtttcccacg cccaggtttt ccacacgcag ttaaccagtc gcccttcccc
    13801 atcaatcagt tgcccggcag catcccagcc cagttcatcc agcccgcgca agatacgcgt
    13861 ttcaaagccc gcctggtgca gcgcctgctc cataaactgc gcgtgatagt tttcctcgat
    13921 atctttgtcc tgcatgatat ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc
    13981 cagttcgtta atcagccctt ccgccggatt gaagccgttg cctttatagc cctgctccgc
    14041 ccaacgttcg aggatcaagc ccgcttcggt atgacaggag gcggagtcgg cgttgtattc
    14101 gtaaaccttc aggccacgct catccatgca gaaatccata cgaccagtga tcatatggtg
    14161 acggcgacgc tgccaggaga gacgcaaacg tggccagagg attttcggaa tgtcgaacag
    14221 cgccagcagg ttgtcatctt tcagcacctt gtcggttgcg tgaagataca tcagatgcag
    14281 ctcgttggtg gctttaatta gctcctgctc ggcactctcg gtaatggtgt agtaatgata
    14341 cggatcctga ttgatcacct gaccgttggc ctgcacatag gcgttttgta gcggatcttt
    14401 ttcatccagc catttaccgt caaactggcc tttgttttcc agacgcgctc cactgatttt
    14461 cagcagctcg cctgcaattt ccggctgcgg taagctgtat tcagtatctt ccgtctggat
    14521 catccagccc agaatggtgg tgtcatcaaa agtgtcttta agggtatagc agccgttttc
    14581 gaccaccatc tccagctcgc gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac
    14641 gttctgttcc gcaatacgga ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc
    14701 agtgtcttta aattcaccgc ctttatccca gataagaagc gcacccgcga ccggcgcacg
    14761 cggcgagccg ttaggaaatg cctgcaatgg caggatgttg tcattaacca cttctcgcag
    14821 gaagcgcagc gagaaaatct cccacgccat acccacgtca gtaaagacca caccgtaatt
    14881 cagaaagaga aaacggcgag caaattcaac gcattgccac ttgtggccca tatattcgtc
    14941 gtcgatatag ctacggaata cggcgtcatc ttcgtattcc tgcggatcga gagaactgta
    15001 atctgaagag tagattgcta ccccacctgg ggcgtagccc aataatgtcc cgaacggggc
    15061 atcctggctg gtcgttcctt tgctcatcac tttaccttta acaatacaac ctaagcagtt
    15121 ggcgtaattg ttgtgctctg attacctgct cagcgacatt aaccggacag aggtcagact
    15181 aatcatacac ctcatcgcag cggctggcgc aaagggttaa aaaattcaca ttctgtacag
    15241 caagtgacct gctacactgc ttcaacacta ccactcagaa ggcaactcac tatgacagac
    15301 aatacttatc agcccgcgaa agtctggacg tgggataaat ccgctggcgg cgcgttcgcc
    15361 aatatcaatc gcccgttttc tggtccgacg catgaaaaaa cgctgcccgt tggcaaacat
    15421 ccattgcaac tttattcgct gggaacgccg aacggtcaga aagtaacgat tatgcttgag
    15481 gagctactgg cgctgggcgt tactggtgca gagtacgacg cctggctgat tcgtattggc
    15541 gatggcgatc aattctccag cggctttgtc gaagtgaacc caaactcgaa gatcccggcg
    15601 ctgcgcgatc atacgcataa tccgccgatc cgcgtgtttg aatctggttc gatcctgctt
    15661 tatctggcgg agaaatttgg ctacttcctg ccgcaggatc tggcaaaacg gactgaaacg
    15721 ttgaactggc tgttctggtt acagggtgcc gcgccgttcc tcggcggtgg ttttggtcac
    15781 ttttaccatt acgcgccggt gaaaattgag tacgccatca accgctttac catggaagcc
    15841 aagcgcctgc tcgacgtgct ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat
    15901 gagtacacca ttgcggatat ggcgatttgg ccgtggtttg gcaacgtggt gctcggcggc
    15961 gtgtatgatg ccgcagagtt tctggatgcg ggaagttata aacatgtgca acgctgggcg
    16021 aaagaagtgg gcgaacgtcc ggcagtgaaa cgtgggcgta ttgttaaccg caccaacggg
    16081 ccgctgaacg agcagttgca tgagcgccat gacgccagtg attttgagac aaatacagaa
    16141 gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa gccatccagg ccggataagg
    16201 cgttcacgcc gcatccggca atcgtgcaca atgcctgatg cgacgctgcc gcgtcttatc
    16261 aggcctacaa aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg
    16321 tctaatgtgg ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc
    16381 agcacccact ggcccagcag atcggcaggg ttaccttcgc aaatcagggc gatattcacg
    16441 tcgcgcttga taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc
    16501 agcacctggc ctggaacgcc aatacacatt gttaactccg gttatttcga ttgccggaca
    16561 aacgcgaaaa agaaccttta gcgggtgttc cctctcaccc taaccctctc cccggagggg
    16621 cgaggggacc gtccgtgcgc tttttaggcg ttgtcctcag tcttattcaa cttcaatact
    16681 tttgacgatc agcgaatcgc cggtatcgac acgcaaccgc tcgccgtgac agagcggaca
    16741 ctgcgcatcg tgctgatgaa tctccaccac ctggctgcaa tcccagcacc aggcctgggc
    16801 gggtttatag acgatatgta aatcgcaccc ttgcgccacc gttccgtggc agacaatttc
    16861 aaaactaaaa cggacggcgc tctcctcaac gcaggagagc gcgccaattt ccagccacac
    16921 ggcggtgacg cgcttaacat cgtgctgctc cgcctgccgt tggataattt caacggcgct
    16981 ctggcaaagc gacaactcat gcattttcgc ccctcctgcg gccaaacagc agggcgcgac
    17041 gccctgcgtg tggtacatcc ggattggtga ctggcaacga aaggatcatt cgtgcgcagt
    17101 catccgtcag gcgttggcct tcttcaatcg acatgctgtg cgaaagcggc gacatcagcg
    17161 aacaggagag gtattgcgaa acaccgtcca gttcgccaac ggtaaaggtc atggtgccat
    17221 acggcagttg cagaccaatt ttttcactca ctttgcgcag cggccagagt tgatccgggc
    17281 cggggaagat aactgcactc agcatccacg gggtgatcac acaccccgtc cactgacctt
    17341 cgaacagcgt aaaatcagaa acatacaccg gcattgaagg atgcagaaaa gagagatcgt
    17401 gcatcgaacg ccgggcaatt tcttcaaacg ctgcctgtac ttgcgccttc ggggaggtct
    17461 ggaaacctgc tatctcttca gtcatgaatc gcctcccgtg ggatggcttc cacgcctgat
    17521 tcgcgcagcg cagccagaac ctgctcaagc gcaggttcaa tcattgcttc aaccgtcggc
    17581 gttaagccga tgtgtggctc cagcgattcc gggatcacgc cgaccagggt cagctttttc
    17641 ggaaactcac cggtgaagcg cagggccgac aatacgtcgg ccaggccaag ctgatgcgga
    17701 gagattttgt tggtaaacaa cgccggaact tcttcatccc gcaggatcat catcgttccc
    17761 ggagtgttct ttttcgacac aatggcatcc gcaataatca gatgatcgcg atttgccatg
    17821 tcgccaagca gctccattcc cgccgtgccg ccatcgagga tctcaacata atccggcaga
    17881 atgtatcgtt gctctaacgc ttcgacaatc cgcacgccga tggcttcatc ggtcagcaaa
    17941 atattgccga cccctaagac taaaatacgc attacagaac cttcactgaa accacttcgt
    18001 tgccgtcggc atccactacg tgtaccgcac aggccatgca cgggtcgaag gagtgaatgg
    18061 tacgcaccac ttccagcggt ttattcggat cggcaaccgg tgtacccacc agcgactgct
    18121 cgtaaggacc gacgtcatca ttgaagttac gcggaccaga gttccaggtt gatggaacaa
    18181 ccgcctggta gttgctgatg ataccgtctt taataaccat ccagtgagag agcataccgc
    18241 gcggcgcttc gaggaagcca acacctttga actcacccgt tgccggaatg ttcggcttca
    18301 caaaggtggt gtgatcgcct ttgccgatat tggtgatcag tgcactgtat tggttttgca
    18361 ggatatcctg caattcacag cagtgaacgg tacgaccaat aatacggccc agcgtggagt
    18421 gcagctgtgc cacttccagc gtgttgccag tcagtttctg ataaatcgca acgatttcat
    18481 tcagtttgtt ctgggtagat tcgcgacctg ccgccagttt caccagcata ttagccagcg
    18541 gacccacttc taccgttttg ccgtagaaag tcggtgattt cacccaggaa tatttcccgt
    18601 cgtcagacca accatcataa gccggaatgg tggtaccttc ccacggcgcc tgtggcgctt
    18661 cgtctttata ccaggagtgc ttcgcgcttt cctgaatgcc tttgatcagg tattcatcgg
    18721 aatgagaagt gatcggacga tacgaggaca gatccgcatt ctcaatgtag cctcccggga
    18781 acaggaagct gccgtttttg ctgtcggtcg ggaattccgg cacgctcagg tagttcaccg
    18841 cacctttacc gcgcgtcagc cattccgggt agaacgcggc gataaccgcg gtatcaacct
    18901 tataaacctg ctcaacaaag tcgctcagtt tgtcgatgaa agacttgatg tacatcaggc
    18961 gctcaaggtt caacacgccc aacccgtcga ggttgattgg gttcgcgaca ccacccactg
    19021 ccaggttctg aatgtgcggc gttttaccgc ccagcagcgc cacgacgcgg ttagcgtcac
    19081 gctggcactc cagcgcctgc aggtagtgcg ctaccgcaat caggttcact tccggcggca
    19141 gtttcatcgc cgggtgaccc cagtagccat tggcgaaaat acccaactga ccgctggcaa
    19201 ccagatcttt gatcttgttc tgaactttag tgaactcttc cggactgttc agatgccagg
    19261 tcgaaacgcc tttcagcatt tcggaggctt tggttgggtc agcttgcagt gcagaagtga
    19321 tgtccaccca gtccagcgcc gaaagctgat agaaatgaac gatatggtca tgcgtggtgt
    19381 gcgcagccag aatgatgtta cggatgtatt gcgcgttaac cggaacgtcg atattcagcg
    19441 cactttctgc cgcacgaacg gaagacagcg cgtgagtggt agtacatacg ccacagatac
    19501 gttgcacaat catccatgca tcgcgcggat cgcggttttt cacgatctct tccatgccgc
    19561 gccacatggt accggaagcc catgctttcg aaacgacgcc attttcgatt tcgcaatcga
    19621 tgcgtaaatg cccctcaata cgggttaccg gatcaatagt aattctctgg ctcatgcttt
    19681 gctcgcctca tgacgattat gatcgttttg ttttaaagga ggaagtatcg gcagtagacg
    19741 aatgagtacg atgtaagcgc aaatctcaat agccacaaaa ccaatagaaa tcaacagttc
    19801 ttcccaggtc gggaagtagg cgtaaccgcc gcccgggttg aatgccacca acgaataggt
    19861 cagacgccag gttgcacaac ctaacagtgc gctcagtgct gacaggaaca gcatgcggga
    19921 atcattacgc agcttcgcca cacgcagaac gaccagcggg aagagcatca gcaggacttc
    19981 aatccagaac atcacggagt agaagtcacc ggcaaacgct aacgacagct tgtcgcgata
    20041 gataagctcg ccaaagcgca gcccgatgaa aatcgccagc aacacactga tggtgttggt
    20101 cagcttgacg aacaggctct tttcatccgg accgttgcca cgcagacccg cctgcaccag
    20161 tgaaccttca aagatgacaa tcgagaagcc catgatgaac gccgtcagca gcgagaacag
    20221 cggcaacatt tcatagctct gccagagcgg atgcaccttg tagcccgccg agatcatcag
    20281 cgaccccatt gaagactggt gcatggtcgg cagcagcgca ccgagcgcga tgatgaagaa
    20341 catcaccttg ttcagacgtt gtagcgacac cttccagccc agacgttcaa acagtgccgg
    20401 agcaaactcc agagccatca cgccgatgta gatggtcata cagaccgccg tctcgaacag
    20461 taccgagttc acgttgaagt gacccggaat gtagaagtac ggcaggctcc agtagcgacc
    20521 cacgtcgata gtgatcgaca agccacccag tgagtaacca aacagactcg ccaacagcgc
    20581 cggacgcacc agcggatggt attgcccacg gttaaagacg tataccgccc acgccagcgc
    20641 ccagccgcca caggcaaagc cggtgccaat cagcaggtca aacgcgatcc acacgcccca
    20701 cgggaagccg ccgttcaggt cagagacaga gcccagaccg aacaccagac gcttcacaat
    20761 caggagcata cagatgacga ttaacggtcc aaaaatcatg accggtttac tgatgatttt
    20821 gccgcccagc ggttgtggat catgactcat gatcgtctcc tccgtcgtga tggtcgtttt
    20881 tggtgttgcg acgaaccagc acggttaagc ccgccagcac agccagtggt agcatcatgc
    20941 ctttatacag ggtgtgttga atattttcgg aacgcgcacc ggtagaaaga tcgtccagtt
    21001 tcggcaggtc gagattttca taaggcacac ccgtcagtac cagaacctga gtaccgccgc
    21061 cctctttctc gccgtacaga tgcggataat atttcggcac cgtatgcagg taagtgtcgc
    21121 cagatttcag cgtctgacgc ggatagtggt attcgctgcc aggcttcagc gccagacgtt
    21181 ttttcgcctc cgccatcagc tcttcacgcg taccgaaaat caccgcgccc gccgggcaca
    21241 cttctacgca gccaggcaga ccgcctttat cgagacgttc cacacctttc tggttgcaca
    21301 gctcgcactt atgcagcgca ccaaacgggt tgttgtagtc gtacttcggc acgttgtacg
    21361 gacaggcgac catgcagtaa cggcaaccgg tgcacacgtc tttgtcgtaa tggacaatgc
    21421 cggttttcgg atcttttttc agtgcagaga ccgggcacac agagacacag ttcggatcga
    21481 cgcagtgcat acactgtttc ttaatgtacg cgtagccgtt ctcctcctgg tctttgttga
    21541 cccctgtgcc gctggtccac acctgaatga tgttattggt atacggcgac agtttgtcgt
    21601 tgttcgacca ggtctgttcc ccttgcgggt tacgttcagg gaagttgata tcctgacact
    21661 tggtgacgca agcctggcag cctacgcaca aggtcgagtc gtacaacatc cccagcgatc
    21721 ccggaattgg cgggcggttt tcagcagccg catgactgac agacggcagc gcgcccgtca
    21781 gcaatgcccc gcaggaggct gctttaataa aattacgtct gttcacggtt attctccccg
    21841 tgagtcagcg ttatctttct tttgctgacg acccagttca cgcaccgcca tcacgctgac
    21901 accggcaacc agcccaacca caccgccgag caaaccaata gcgcctgcag agacgttgcc
    21961 gccctcttta gcgttaacat ccggtttctg tgaacgcgga gtctgatttt cgacgttggc
    22021 aagctgatgg atgcctttat ggaagccgat acctttttcg ttacagccat agcacgggtg
    22081 accaatcgcc accggccaca caccgccaac atcgcagaat tgcagcgttg agcagttgcc
    22141 gtaagtttct ggccctttac agccgaggtg gtacaggcac cagccttcgc ggtggccttc
    22201 atcaccgaac tctttggcaa aacgaccagc atcgaagtgc gggcgacgtt cgcagtgttc
    22261 gtgaatcaga cggccatagg cgaaggtcgg acggtttttg tcatccagtt tcggcggttt
    22321 gccgtaagtg atgatgtgcg caacggtcgc gaggaagttg tgcgggttcg gcgggcagcc
    22381 cggaatattg ataacggttt tgcctggcag aacttcttgc aagctgactg cgccagttgg
    22441 gttaactcca gctgcggcaa caccgcccca cgcggagcag gaaccaatag cgataatggc
    22501 tgctgagcct tccgccgctt tgcggatgtg atccacaatc ggctcaccgg caaccatgca
    22561 ataaataccg ttatctttta atgggatgga accatccacc actaacacat actgcccttt
    22621 gtacttctcg agagcgttat gtttgttctc ttcgacctga tgaccgaagg cggcggaaag
    22681 cacttcgtga tattccagag agatagtctc cagcacgagg ttttctaccg ttggatgcgt
    22741 tgcacgaagc agagattccg tacaaccggt gcactcctgc gcgccaatcc agataactgg
    22801 cggacgctgc gggttagtaa ccgattcggc catctctgca gcggctttgc tacttaaccc
    22861 catggtggcg gctaatgctg cacaaagctt catgaaatca cgacggttaa tgccgtgaga
    22921 atggatgagg gtgttatctc cagtcattta tagttattcc gttgcgagga cctggcatat
    22981 attttgcctc aatcgcaaaa tcaataatgc gatcgatgcg ccatttacca cacatttatt
    23041 atggttatcg atatcatgat actgcggcgg ggcgctaaaa cgaagggaag aagcattagt
    23101 gtaattaatt aaagcaagat aatacgtatg tttgatcaat tttcgaggca ataaccggcc
    23161 ataaaaaagc gcaacgtaga acaggaatta ttctcacgaa agaaatccat taccatctct
    23221 ttatccagtc catagcggcg cgtcagaata cccgcttccc aggcgctaag ctggcgatct
    23281 ccggtctttt cgtacatccg gtgtgagtag cctaatacaa aaccgcgttt ataatcggca
    23341 cagaattttg tcacattaac cgcactgtcg gcatgcgttg cttttaatcc cgccattaag
    23401 cctttaccaa aatggttatt catcgttcac ctcattcgtt atatatcgcc atatatagcg
    23461 atattttagg caaagaacga cttttgcgca cgctattttt tcatacttgt ttaagacgta
    23521 atcccgtaag agataaacct atcaggagag atatttatcg aaccatttca gtgtcctttg
    23581 ccaggcaaga tcggcggcag atttgtcata acggggcgtg gaatcattat ggaatccgtg
    23641 attaaccccc ggatagatat aagcctcata aaccttatta ttgtctttca acgccgcctc
    23701 gtaaggaggc cagccctcgt tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg
    23761 cgcttcaatc ttcgccacat cggcagtggg tgcctgacga ccataaaacg gcaccgcgca
    23821 ggccagttcc ggatacgcga cagccgccgc gttcgatacg ccaccgccat agcaaaatcc
    23881 ggtaataccc actttacctg tcgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa
    23941 gaaatcattc atcagtttgg ttggatcaac ctgctgttgc agctcacgac ctttatcatc
    24001 atttcccgga taacctccaa cggaacttaa tccgtcaggt gccagggcga tataccccgc
    24061 cttcgccact cgccgtgcca catcttcgat atacggattc agtccacgat tctcatgcac
    24121 cacccccacg gctggcgttt tgccgctcat ttttgcgggt ttcaccagat aaccccgtac
    24181 ctcgccgtga ccatttggcg aaggatacgt gatgtactca gcaacaatct ccgggtcggt
    24241 aaactctacc tgagtcgcca gcgcataatt tggcttgagc aaatcaaaca acgctaatgc
    24301 cgtcatcccg cccaccgcat acttcgccgc aaggttgagg aactcacgtt tcgaaatttt
    24361 cccgtgaacg taatagtcgt agtaatccaa caactcttgt gggaaatctt tggcggttaa
    24421 acgcggcatc gttgcattcc tcaactggtg tttttttaag caaagcataa gcgcgtgttt
    24481 ttgcccagtt tttcgtcact ctgtgagcca gactacggga tacgcgctgg cgaatcgcta
    24541 aactagaaac attgtttcga aattgaacgg aggaaaggag aggtcatggt ctggttagcg
    24601 aatcccgaac gttacgggca gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg
    24661 cccgcattat cgctcggttt atggcacaat ttcggtcacg ttaacgcgct ggaatcacag
    24721 cgtgcaatcc tgcgtaaagc gtttgattta ggcattacgc actttgattt agccaacaat
    24781 tacgggccgc ctccaggaag cgcagaagag aactttggtc gcctgctgcg cgaggatttt
    24841 gctgcttatc gcgatgaact gattatctct accaaggctg gctacgatat gtggcccggc
    24901 ccgtacggct ctggcggttc acgtaaatac ctgctcgcca gcctcgacca aagcctgaag
    24961 cgtatggggc ttgagtatgt cgatatcttt tactctcatc gcgtcgatga aaatacgccg
    25021 atggaagaaa ccgcctctgc gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc
    25081 gggatctcct cttactcgcc agagcggacg caaaaaatgg tcgagttgct gcacgagtgg
    25141 aaaattccgc ttttaattca tcaaccttcg tacaatttac tgaaccgctg ggtggataaa
    25201 agcggcctgc tggataccct gcaaaataac ggcgtgggct gtattgcctt tactcctctg
    25261 gctcagggat tgctgaccgg aaaatatctc aacggcattc cgcaagattc acggatgcat
    25321 cgtgaaggga ataaagttcg tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac
    25381 agcctgcgct tattgaatga aatggcacag cagcgtggac aatcaatggc gcaaatggcg
    25441 ttaagctggt tgctgaaaga tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg
    25501 gagcaactag aggagaacgt gcaggcgctg aataatctga catttagcac cgaggagctg
    25561 gcgcagattg atcagcatat cgccgatggc gagctgaatc tgtggcaggc gtcgtccgat
    25621 aaatgacctg ttaataacgg gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg
    25681 agtcagtcgg tcaagatagc ccatcacaaa tgcagaaagc acaaacgtca gatggataat
    25741 gacgtaccac atcagtttgt tatccaggac atttttcgca tccataaaga cgcgcagtaa
    25801 gtgaatggaa gaaattgcca caatcgacgc tgctactttg tttttcagcg acgttgcgtc
    25861 cattttcccc agccacttca gcttctcttt gttctcggag atatccagct gtgagacgaa
    25921 attctcataa ccggaaaaca tcaccatcac cagcaaaccg ccaaccagcg tcatatccac
    25981 cagcgacagc aacacgagga tcaaatctga ttccgccatc gagaagatat tcggcagtac
    26041 gtgaataatc tcctggaaga acttcagcgc cagggcaact aacgccagcg aaaggccaaa
    26101 gtacacgggg gcaagcagcc agcgagaagc atacattgca ttttcaagaa aacgttccat
    26161 acattcctgt cgtgtagtta aaacgggcaa cagtatatcg caattgagta aacagcacgc
    26221 aacagcaaat caacatatac gttaaacctt tgtcgcataa ttctgaggtt tagggatctt
    26281 cctggttccc ttatcaacgc atcgagagcg tctaccctta aaggacgttg aacctgaagg
    26341 gagaaaaacg atgtctcatt taaaagaccc gaccacgcag tattacactg gtgaatatcc
    26401 caaacagaaa caaccgacgc caggcatcca ggcgaagatg acaccgatac cggattgcgg
    26461 cgagaaaacc tatgttggta gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg
    26521 cgattccgga ataggtcgcg ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc
    26581 gatcagttat cttcccgtgg aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga
    26641 atgcggacgc aaagccgttc tgctgccagg cgatttaagc gatgagaaat tcgcccgttc
    26701 gctggttcac gaagcgcaca aggcgttagg cgggctggat attatggcgc tggtcgccgg
    26761 gaaacaggtt gccattccgg atattgcaga cctcaccagc gaacagtttc aaaagacctt
    26821 tgccattaac gttttcgcgc tgttctggct aacccaggaa gcgatccccc tgctaccgaa
    26881 aggtgcaagt gtcatcacca cttcgtcaat ccaggcatac cagccaagtc cgcatttact
    26941 ggactatgcg gctacgaagg cggcgattct gaactacagc cgtggcctgg caaaacaggt
    27001 cgcggagaaa ggtattcggg tgaatattgt cgcgccaggc cctatctgga cggcactgca
    27061 aatttccagc ggacaaacgc aggataagat cccgcagttt ggtcagaaaa cgccgatgaa
    27121 gcgtgcgggg caaccggcgg aactggcccc tgtatatgtt tatctggcaa gtcaggagtc
    27181 gagctacgtc accgcagaag tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa
    27241 tgcccggttg tgaaaagcaa ccgggcatca ttgtgaattc actcttacct gatgtggcga
    27301 aaacgcctta tccgcctaca aacgcatgaa gattcaacat gttgcaatag attttgtagg
    27361 cctgataagc gtagcgcatc aggcattgag cacctaatgc cggatgcgtc gtgaacgcct
    27421 tatccggcct acaaacgcac gaagattcaa catgttgcaa tagattttgt aggcctgatg
    27481 agcgtagcgc atcaggcaat tctactttac ttcgctttgg cggtttcttc gccgaccaga
    27541 cctatcttca ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa
    27601 tcgacggttt tgtccgctcg gaagaagatg gtggtgtctt tcttgccttc ggttaacgca
    27661 ttcaacgccg taatcattgt ttcatcggtg accggatcgt taccgataaa catcgagttg
    27721 tctgccttca ccgacagata aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa
    27781 gcaggcaagt tcaccttcac atctaccgtc gctaacggtg ccgccaccat aaagataatc
    27841 agcagaacca acatcacgtc gataaacggc gtcacgttga tatcatgcat ttcgccgtta
    27901 tcgtcgaggt tttcgttaag atgcattgcc atcggacatt atcctgcgcg taatttttgt
    27961 gcgacacgaa ccggatgcgc agcggcgctg gcttccagat ccaggtcacg gctttgcagc
    28021 aacaatacct gcgctgcaac atcacccagc atcgctttaa agccgccaat ctggcgtgca
    28081 aatacgttat agataacgac cgcaggaatc gctgccacga ggccgattgc cgttgctaac
    28141 agagcttctg cgatacccgg cgcaacgact gccaggttag tggtctgcgt ttgcgcgata
    28201 ccaataaagc tgttcatgat gccccagacc gtaccaaaca gaccaacgaa cggagaaatc
    28261 gcgccgatgg ttgccagata gccgttaccg cggcccattt gacgacccac tgcggcgacc
    28321 cgacgctcca gacggaagct ggtacgttct ttaatacctt cgttatcgtc gctgccttct
    28381 gacagttcca gctcattctg cgcttcattg agcaaatgca ggcttaagct tttgctacca
    28441 aaatcagcgg cgatatcgtt ggcctggttt aaggaacgtg cttcagccag cagttgctgc
    28501 tcgcgcttaa ggcgacgctt ctgattgaag aactctacgc tcttactgaa gaagattgcc
    28561 caggtgacta cagaggccaa aataagccca atcatcacgc acttaacgac aatatcggcg
    28621 tactgataca taccccagac ggaaaggtcc gtctgcatta aattattacc cactgtgtat
    28681 ctccaggacg caagtcacaa aatctgcgca taataatatc aaaacgacgt cgaattgata
    28741 gtcgttctca ttactatttg catactgccg tacctttgct ttcttttcct tgcgtttacg
    28801 cagtaaaaaa gtcaccagca cgccatttgc gaaaattttc tgctttatgc caattcttca
    28861 ggatgcgccc gcgaatattc atgctagttt agacatccag acgtataaaa acaggaatcc
    28921 cgacatggcg gacaaaaagc ttgatactca actggtgaat gcaggacgca gcaaaaaata
    28981 cactcttggc gcggtaaata gcgtgattca gcgcgcttct tcgctggtat ttgagagtat
    29041 ggaagccaaa aagcacgcga cgcgcaatcg cgccaatggc gagttgttct atggacggcg
    29101 cggaacgtta acccattttt ccttacaaca agcgatgtgt gaactggaag gtggcgcagg
    29161 ctgcgcgcta tttccctgcg gggcggcagc ggttgctaat tcgattcttg cttttgtcga
    29221 acagggcgat catgtgttga tgaccaacac cgcctatgaa ccgagtcagg atttctgtag
    29281 caaaatcctc agcaaactgg gcgtaacgac atcgtggttt gatccgctga ttggtgccga
    29341 tatcgttaag catctgcagc caaacactaa aatcgtgttt ctggaatcgc cgggttccat
    29401 cactatggaa gtccacgacg ttccggcgat tgttgccgcc gtgcgcagtg tggtgccgga
    29461 tgccatcatt atgatcgaca acacctgggc agccggtgtg ctgtttaagg cgctggattt
    29521 tggcatcgat gtttctattc aggccgccac caaatatctg gttgggcatt cagatgcgat
    29581 gattggcact gccgtgtgca atgcccgttg ctgggagcag ctacgggaaa atgcctatct
    29641 gatgggccag atggtcgatg ccgataccgc ctatataacc agccgtggcc tgcgcacatt
    29701 aggtgtgcgt ttgcgtcaac atcatgaaag cagtctgaaa gtggccgaat ggctggcaga
    29761 acatccgcaa gttgcgcgag ttaaccaccc tgccctgcct ggcagtaaag gacacgaatt
    29821 ctggaaacga gactttacag gcagcagcgg gctattttcc tttgtgctta agaaaaaact
    29881 caatgatgaa gagctggcga actatctgga taacttcagt ttattcagca tggcctactc
    29941 gtggggcggg tatgaatcgt tgatcctggc aaatcaacca gaacatatcg ccgccattcg
    30001 cccacaaggc gagatcgatt ttagcgggac cttgattcgc ctgcatattg gtctggaaga
    30061 tgtcgacgat ctgattgccg atctggacgc cggttttgca cggattgtat aacattgcca
    30121 cttttggaca attttgcaga cattttattg tgaaaagtct taaattgttg cgtccgggat
    30181 caaggcgtcc cggacgattc aggagtacaa taggcagata aaggcttaaa cgctgttcca
    30241 caggaaagtc catggctgtt attcaagata tcatcgctgc gctctggcaa cacgactttg
    30301 ccgcgctggc ggatcctcat attgttagcg ttgtttactt tgtcatgttt gccacgctgt
    30361 ttttagaaaa cggcctgctg cccgcctcat ttttgccagg cgacagcttg ttgatactgg
    30421 caggcgcatt gattgcccaa ggggttatgg attttctgcc tacgattgcg attctgaccg
    30481 ccgcagcaag tctgggctgc tggctaagtt atattcaggg gagctggtta gggaatacca
    30541 aaacggtgaa aggctggctg gcacagcttc ctgctaaata tcaccagcgc gccacctgca
    30601 tgtttgaccg ccacggtctg ctggcgctgc tggctggacg ttttcttgca tttgtccgta
    30661 cgctgctgcc aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc cagtttttca
    30721 actggttaag tggattgctg tgggtcagcg tggtaaccag ttttggctat gccttaagta
    30781 tgattccgtt cgttaaacgc catgaagatc aggtaatgac gttcctgatg atcctgccaa
    30841 ttgccttgtt aaccgctggc ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact
    30901 gtaacgcctg acgattttcc ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc
    30961 tgcatcgccc gcattcttgc cgcatcttcc cccggcgtca caccgaagta acgtttaaac
    31021 tcacggctaa attgcgatgc gctttcatag ccgacgcgca tcgctgctgc gctggccttc
    31081 atgccgtcat ggatgatcat catccgcgcc ttatgcagac ggtaattctt caaatactgc
    31141 aacggcgagg tgctggtgac agacttaaaa ttatggtgga acgccgatac gctcatgttg
    31201 gcttctgccg ccagttgctc gacgctcagg ttttcggtgt atttattctc aattcgtttc
    31261 agcacgcggc taatcagact gaagtgagtc tggcgactga ccagcgccag taacgcgccg
    31321 ccgcaaggtc cggtcagcac gtagtacaga atttcgcgga tgatctgttt gccgagaata
    31381 cgcgcatcca gtggtcgctc catcacgtcg agtaaccgct ccgccgcgca taaaatctct
    31441 tctgataacg tggcggagtt aatcccgctg gctgccatcg acggctggaa atgctcatct
    31501 tcgccaatgt ccatcaacag ttcctgtaac tgcaaaatat cgacattgag acgcaaccct
    31561 gccagcggca cctctgacgt ggcataggtt tcgcactcaa acggcaacgg caccgtcagc
    31621 agcaggtatt cattggcatc ataacgaaac acgcgttcat tgatataacc gattttatgc
    31681 ccggaaaaga gaattatgat gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc
    31741 tcgccataca acaaacgcac atcgggcaac agttctgaca aactattttc tttatttttc
    31801 agtttattaa ctttatccgc cagcaagcga caaatctctt cacgtttcat atcgcgtaat
    31861 ttcttaggaa taatgcggca atttgattgt gcgcaatttt gtagcatttc tccagcactc
    31921 tggagaaata ggcaagacat tggcagaaat gagcattgag agccagggcg ctggcgatca
    31981 caatgaaaaa catcaggcag atcgttctct gccctcatat tggcccagca aagggagcaa
    32041 gtaatgaaca actttaatct gcacacccca acccgcattc tgtttggtaa aggcgcaatc
    32101 gctggtttac gcgaacaaat tcctcacgat gctcgcgtat tgattaccta cggtggcggc
    32161 agcgtgaaaa aaaccggcgt tctcgatcaa gttctggatg ccctgaaagg catggacgtg
    32221 ctggaatttg gcggtattga gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa
    32281 ctggttcgcg aacagaaagt gactttcctg ctggcggttg gcggcggttc tgtactggac
    32341 ggcaccaaat ttatcgccgc agcggctaac tatccggaaa atatcgatcc gtggcacatt
    32401 ctgcaaacgg gcggtaaaga gattaaaagc gccatcccga tgggctgtgt gctgacgctg
    32461 ccagcaaccg gttcagaatc caacgcaggc gcggtgatct cccgtaaaac cacaggcgac
    32521 aagcaggcgt tccattctgc acatgttcag ccggtatttg ccgtgctcga tccggtttat
    32581 acctacaccc tgccgccgcg tcaggtggct aacggcgtag tggacgcctt tgtacacacc
    32641 gtggaacagt atgttaccaa accggttgat gccaaaattc aggaccgttt cgcagaaggc
    32701 attttgctga cgctaatcga agatggtccg aaagccctga aagagccaga aaactacgat
    32761 gtgcgcgcca acgtcatgtg ggcggcaact caggcgctga acggtttgat tggcactggc
    32821 gtaccgcagg actgggcaac gcatatgctg ggccacgaac tgactgcgat gcacggtctg
    32881 gatcacgcgc aaacactggc tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc
    32941 aagcgcgcta agctgctgca atatgctgaa cgcgtctgga acatcactga aggttccgac
    33001 gatgagcgta ttgacgccgc gattgccgca acccgcaatt tctttgagca attaggcgtg
    33061 ccgacccacc tctccgacta cggtctggac ggcagctcca tcccggcttt gctgaaaaaa
    33121 ctggaagagc acggcatgac ccaactgggc gaaaatcatg acattacgtt ggatgtcagc
    33181 cgccgtatat acgaagccgc ccgctaagct ttttacgcct caaactttcg ttttcgggca
    33241 tttcgtccag acttaagttc acaacacctc accggagcct gctccggtga gttcatataa
    33301 aggaggaacg tatggctaat ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc
    33361 agctgggact gggcgtctgg caagcaagta atgaggaagt aatcaccgcc attcaaaaag
    33421 cgttagaagt gggttatcgc tcgattgata ccgccgcggc ctacaagaac gaagaaggtg
    33481 tcggcaaagc cctgaaaaat gcctcagtca acagagaaga actgttcatc accactaagc
    33541 tgtggaacga cgaccacaag cgcccccgcg aagccctgct cgacagcctg aaaaaactcc
    33601 agcttgatta tatcgacctc tacttaatgc actggcccgt tcccgctatc gaccattatg
    33661 tcgaagcatg gaaaggcatg atcgaattgc aaaaagaggg attaatcaaa agcatcggcg
    33721 tgtgcaactt ccagatccat cacctgcaac gcctgattga tgaaactggc gtgacgcctg
    33781 tgataaacca gatcgaactt catccgctga tgcaacaacg ccagctacac gcctggaacg
    33841 cgacacacaa aatccagacc gaatcctgga gcccattagc gcaaggaggg aaaggcgttt
    33901 tcgatcagaa agtcattcgc gatctggcag ataaatacgg caaaaccccg gcgcagattg
    33961 ttatccgctg gcatctggat agcggcctgg tggtgatccc gaaatcggtc acaccttcac
    34021 gtattgccga aaactttgat gtctgggatt tccgtctcga caaagacgaa ctcggcaaaa
    34081 ttgcaaaact cgatcagggc aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac
    34141 gtgcaaattc tcccggtggc ggtaatgttc cgctaccgga cttttcagaa atcatttatt
    34201 cccctcgcgt cccgcccgtt gttactcttc cttgttcagg aatgccaaat ataaggacat
    34261 catcatgcag agccggaagc tcttaaaaga acaactcatc tatatccggg ataaacgcaa
    34321 cggagaggtg aaaaacagat gaaaataata cttctgtttt tagcagccct ggcaagtttt
    34381 accgtacacg cgcagcctcc ttcacagacc gtagaacaaa cagtccggca gatttatcag
    34441 aactataaat cagatgccag tacgccttat ttcggtgaaa ccggagagcg ggcgataact
    34501 tcagcgcgta ttcaacaggc gcttaccctg aacgacaatc ttacactacc gggcaatatt
    34561 ggctggctgg attatgatcc ggtttgtgat tgtcaggatt ttggcgatct ggtgttagaa
    34621 agcgtagcga taacccaaac tgacgtcgat catgccgatg ctgttgtgcg ctttcgtatc
    34681 tttaaagatg ataaagaaaa gaccacacag acactgaaaa tggtggcgga aaatggtcgt
    34741 tgggtcattg atgatattgt cagcaatcat ggcagcgtct tacaagcagt taatagcgag
    34801 aatgaaaaaa cgctggccgc tttagcttcg ttgcagaaag aacagccaga atcctttgtt
    34861 gctgaactct ttgaacatat tgctgattat agctggccgt ggacgtgggt ggtttccgac
    34921 tcttaccgcc aggcggttaa tgccttctat aaaaccacct tcaagacggc caataatccc
    34981 gatgaagata tgcaaataga acggcaattt atttacgaca atccgatctg ttttggcgaa
    35041 gagtcgctat tttcacgcgt tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc
    35101 cgcattcatg ttcgttttac gctgaccaat ggcaacaatg aagagcaaga gctggtttta
    35161 cagcggcgcg aaggtaagtg ggaaatcgct gattttatcc gcccgaacag cggcagccta
    35221 cttaagcaga ttgaggcaaa aaccgccgcc agattaaagc aatgagctga attaaataac
    35281 aattagccgg aacaataaat aaaagggaac actatatgaa aacgattttc accgtgggag
    35341 ctgttgttct ggcaacctgc ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcaac
    35401 tggcgagcaa tgtacaaaca ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag
    35461 cactacgccc acaaatctat gctgccaaat ccgaagctaa cagagccaat acgcgtcttg
    35521 atgcacagga ctattttgat tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa
    35581 atccccggca gcatgtcagt tgccggggat tttttttaac gtccaaccgc cgctttaggg
    35641 cgtttcttcg caccagcatt caccggacga gattgcgtag acgacgcttt ttttgccgta
    35701 gcaggcgtct gacgctgagt cgccatcggc gtatgtttag tcaacgccgg acgggtattg
    35761 cggttctggc gacgagcttc acgcatctct tcaatggttg gcgcaggcac taagcaatcg
    35821 cgacggctgc caatcagatg ctttttgccc atcgcttcca gcgcctggcg gattaacggc
    35881 cagtttgccg gatcgtggta acgcaacaac gctttatgca aacgacgctg tttgtcgccc
    35941 ttcggtacga agacgccttc gctcttataa ccaatcttcg ccagcggttt tttcccggtg
    36001 taatacatgg tggttgagtt cgccagcggc gatgggtaga agttctgtac ctggtcgaga
    36061 cggaaacgat gctttttcag ccacagcgcc aaattcacca tatcttcatc acgcgtaccg
    36121 gggtgcgcag agatgaaata cgggatcaga tactgctctt tacctgcctg tttcgagtag
    36181 gtatcgaaca gctctttaaa gcggtcatag ctgcccatgc ccggcttcat catcttcgac
    36241 aacggccctt cttcggtatg ttccggagca atcttcagat aaccgccgac gtgatgggtc
    36301 gccaactctt tgatatagcg cggatcttcc acggcgatgt cataacgtac gcctgaggcg
    36361 ataaggattt ttttaatgcc tttcagatca cgcgcacggc gatagaggtt gatcgtcggt
    36421 tcgtggttag tgtccatgtg cggacaaata tccggataaa cgcacgacaa acggcgacaa
    36481 gtttgttcag cgcgtggcga tttgcagcgc aacatataca tgttggcagt tggcccaccg
    36541 agatcggaaa tcacgcccgt aaaacctgga acggtgtcgc ggatcgcttc gatctcatta
    36601 atgatcgaat cttccgaacg gctctgaata atgcgccctt cgtgctcggt aatagaacag
    36661 aaagaacagc cgccaaagca gccgcgcata atgttgaccg aaaaacggat catttcgtaa
    36721 gccggaatac gggcattgcc ataggccgga tgtggcacgc gcttgtacgg cagcgcaaaa
    36781 acgctgtcca tctcttcggt agaaagcggg atagcaggcg ggttgatcca cacatagcgg
    36841 tcaccgtgtt tttgcatcaa tgcgcgggcg cagcctgggt tggtttcgtg gtgcagaatg
    36901 cgcgaagcat gggcgtacag cactttatcg cccttcactt tctcgaaaga aggcagcaat
    36961 acgtaggttt tttcccacgg tttctggcgc ggtggctgca cgattacagc tttcgcttcc
    37021 tgctttttcg gcgcaacagg tttgttatcc gcacacggca aatcttcgcc atacggatgc
    37081 gggattgggt cgattttccc tggggtatca agacgggtgg aatccacacc gctccagcca
    37141 ggcagcgcct cttttacgat aattgcggta ttacgcacat cgcggatttc actaatcggc
    37201 tcgcccatcg ccagacgatg cgctacttcc accagcggac gttcaccgtt accaaacatc
    37261 agcatgtcgg ctttcgaatc taccagaacg gaacggcgca cggtatcgga ccagtaatca
    37321 taatgcgcgg tacggcgcag gctagcctca ataccgccga ggatcaccgg cacatctttc
    37381 cacgcctctt tacaacgctg ggtataaacc agcgtggcgc gatccggtcg cttacccgcg
    37441 acattatctg gcgtgtaggc atcatcatga cgtaaacggc gatcggcggt atagcggttg
    37501 atcatcgaat ccatgttgcc agcagtaaca ccaaaaaaca gattcggttt acccagacgc
    37561 ataaagtcgt ctttgctgct ccagtccggc tgggcgatga tcccgacgcg aaagccctgc
    37621 gcttccagca tacgaccgca aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg
    37681 ccagtaacca aaatgatgtc gcagctatcc cagccaagtt gatccatctc ttcacgagac
    37741 atcggcaaaa acggtgccgg tccaaaacag gcggcccagt actgcggcca ggagaacagg
    37801 tcgcgatccg gttggatcag ggagatagag ctcattttgc ttccagaaat gataaaaaaa
    37861 taatcaaagg ccggggatta taagccggaa cgaaagagaa atcgaaaggt attccatact
    37921 cgccctcctc gggcgagtat gaagattacg gtaccggatt gaccaacagt tgcccaatcg
    37981 agccacggtc cgccatttcc agcgtctgac tgttgaagta gaacgggaag tgcgcccagg
    38041 aaggctgacc gaaataaaca agcagctcca cttgtccgtc aacccaaacg gtatctttcc
    38101 agcctctgtc ttccgggaac ggcatcgcgc cattcacgtt acggatctgg aacattacgc
    38161 cttcaatatg gaacgcttgc ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt
    38221 gctgcgcggt gacatcaata cggttgacgt cccacagctg tccattaata cccgggtcat
    38281 cacccagact gatatcgcga ctgcgaattg gcgaaccagc catgatttca gttggcagca
    38341 agcgcatcgg aagactgtct gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca
    38401 ccaaggtaga aaccagaata ctggatggct caaagaagcc gcgaatacga tcaacaatgc
    38461 tcgccgcttc accacaggtg atcgacactt catcgccgtt gctcatatcc accagaatct
    38521 cgcggcgctc gcccggtgcc agcgaaagtt gcttcaccga tacaggagca ggcaggaatc
    38581 cctgatcgcc agaaatcaca tgtaacgggc gaccatcgct catctgtagt tgatagcgac
    38641 gagagttcga cgcgttcagc agtcgcaagc gcacccagcc acgcgagact tcaacgtacg
    38701 ggctttgtac accgttaacc agcagcgtat caccaacaaa gccgccgctt cccggttcgt
    38761 tgtattctgg cgtaccaaag ttatccagcc gtttatcctg gataatgacc ggaaaatcat
    38821 ccacaccata atggttgggg ataggcagcg acttgctgac ttcatcttcc accagccaca
    38881 ttccggcaag gccgttatag acctgctgag ccgtgcggtt gggagtattg gcgtgatacc
    38941 acagagtagc tgcgttctgg cgaataggca gtacgggtgc ccagtcagcg tttggcgaca
    39001 tcatccgtgc cggaccgccc atcagcgggc ctggtacctg tagcccggcc accgtcattg
    39061 agacattttc tgtcaggcgg ttgctgtaaa taagcttaac atcgtcgtcc ttccagacgc
    39121 ggatagtcgg ccccaggtaa cgaccattga ttccccagac cgacgcgcgt gtccctggcg
    39181 taaatgacca gtgcgcacgt tgtacagtca taaacagcgg ttgcccacgg cgagattcaa
    39241 gtagcggcgg aacgggtagc ggttgctgtt gcccggctgc gctggccttc aggggaacag
    39301 cgcctgcaca aagtgcaatc cccgatgcct gaatgaactg acgccgactg agtgacatat
    39361 ttgctccatg taaaactggc taaagtaata ccgtgcagga aagtgttccc cgcaaaaata
    39421 cagattgccg acttaaactt ttccggcggc ttcgcgttct gcgacttctt tatcgagctc
    39481 ggcgattttt tgttccatta tcgaacgaca atgggcagcc agctcacgaa cctgatcttt
    39541 gccatactga ctgacgtcaa ttggcggcag catttcgaca atcaccagac cgttgtgcag
    39601 tcgattaaga ttaatcttat tcgaagttgt agagacgcac acgggaataa tcgggacgcc
    39661 cgccgcaatt gccgcgtgaa atgctccagt cttgaacggt agcaggccgc gaccacggct
    39721 gcgggttcct tccgggaaca tccagatgga aatacggcgt tttttgaaat gattcacgac
    39781 ttccgcaatg gtgccgtgag ctttagtgcg attgtttctg tcgatcaata agttgccggt
    39841 taaccagtac aactgcccga agaaggggat ccacagcaag ctctttttac ctaccgtcac
    39901 cgtcggcggt tgcacaatgt tcgatgctgt caccatgtca tagttgttct ggtggttagc
    39961 gatatagata gcattgccgt agctttcagc atctgcaggt ttgcggcact caactttcag
    40021 gccgaacagt ggcgcaagac ggccaaacat atgcccaaag gtggccacat gtttcgggtt
    40081 acgcgggctg aaaaggcagt aaatggagcc gaatacacag actaagatgc tgtaaatcac
    40141 ggtaataata agacgaaaga tatatagcat aacaccctct gaaggtctga gagactggca
    40201 ttgtacgtca cctgaaatca ggttagggca ttgttaaaag ccgctctctg gaaaagcgta
    40261 ttgttaatcg caacgcatga ataaacaacg gtttcaaggg aatttttatc gtttccgggg
    40321 gttgaatacg ctgccggatg acgacttaac gtttcatccg gcgttccttg ctagcgggag
    40381 gaaacagcgc cctccccggc atattactct tcgctatcac cgctgctggc acggcgagga
    40441 gagtcgatct caacacgatc gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca
    40501 cgttcgccag tgactttctg tagctcttcc ggacgcagtt taattttgcg tttcccaaca
    40561 tgaatggtca gcgtgctttg cggcggcaga acgtacagtt gcgccagacc atcctcgcca
    40621 cgcgcggctt ctgccgatgg aatgttgata atcttgttgc ctttgccctt cgacagctgc
    40681 ggcagatcgc ttaccgggaa catcaacata cggcctgcct gagtgattgc cagcagcata
    40741 tcggaagcat cttcaatcac caccggcggc ataacatggg cattttccgg taaggtgatc
    40801 aaagccttac ccgcacggtt acgcgctacc agatcgttaa aggtgcagac gaaaccgtaa
    40861 cccgcatcgg aagccatcag cagtttctga tcgtcgcttt ccatcagcat atggtcaacg
    40921 gtcgccccag gcggcaacgt taatttgccg gtgagtggtt cgccctgacc acgcgccgac
    40981 ggcagcgtaa tcgggtcgat ggcatagcta cgaccggtgg aatcaacaaa cactaccggt
    41041 tggttactct tacctttcac cgccgctttg aagctatcac ccgctttata attcaggccc
    41101 ggcgcgtcga tatcatggcc tttagcgctg cgtacccagc ccatctgcga cagcacaatg
    41161 gtgacaggtt cagacggcag catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc
    41221 tgcaacggcg aacgacgatc gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc
    41281 agcaggttat tcattttacg ctcggaagcc aaaatgccct gcaactggtc gcgctctttt
    41341 tccagctcac tctgctcacc gcgaatcttc atctcttcca gtttggcaag atgacgcagt
    41401 ttcagttcga ggatcgcttc cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc
    41461 ggtttcggtt catcttcgtt acgaatgatc tcaatcactt cgtcgatgtt gagaaacgcc
    41521 accagcaaac cttccaggat atgcaggcgc ttgaggactt tctccagacg atagttcagt
    41581 cggcggcgca cggtatcgcg acggaacacc agccattcgg agaggatttc cagcaggttt
    41641 ttcaccgccg gacgaccatc cagaccgatc atattgaggt taatgcgata gctcttttcc
    41701 agatcggtgg tagcgaagag gtggttcatc acctgatcca tatccacgcg gttggaacgc
    41761 ggcacaatca ccagacgggt cgggttctcg tggtcagatt catcgcgcag atcgtcaacc
    41821 atcggcagct ttttgttgcg catttgcgca gcaatttgct ccagtacgcg cgcacctgaa
    41881 acctgatgcg gtaatgcgct gataaccacc gcgccatctt ctttcttcca caccgcgcgc
    41941 atacgcactg aaccacgtcc gttctcgtag attttacgaa tctcggcgcg cgaagtgata
    42001 atttccgctt cagtcggata atccggcccc tgcacgatat ccagcagctg atcgagcgta
    42061 gttttcggct ggtcgattaa tgcgattgcc gcctgagcta cttcacgcag gttatgcggt
    42121 ggaatatcgg tcgccatgcc gacggcaata ccggtggtgc cgttaagcaa aatgtttggc
    42181 agacgggcag gtagcatttt cggctcctgc aaagtgccgt cgaagtttgg cacccagtca
    42241 gccgtcccct gccccagctc gctcaatagc agctcggaat atttcgacaa ccgggattcg
    42301 gtgtaacgca ttgccgcgaa cgatttcgga tcgtccggcg cgccccagtt cccctgacca
    42361 tcaaccagcg gataacggta agagaacggc tgcgccatca ggaccatcgc ttcataacag
    42421 gcgctatcgc cgtgcggatg gtatttaccc agtacgtcac cgacggtacg ggccgatttt
    42481 ttaaatttgg cgctggcatt caggcccagt tcagacatcg catacacaat gcggcgctga
    42541 acaggtttca gaccatcacc aataaacggc aacgcacggt ccatgatcac gtacatggag
    42601 tagtttaagt aggcgttttc cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc
    42661 attaattctg attcctcaac ttattcgcca gccttcaaag gggcaatatt gccggagata
    42721 ctacattatc tgccgcgtcg agtcacagga gatcagcgtg aatagcgccg ccggacgtgc
    42781 tccgacggcg ataaaggcat caatgtgcct tgatatacaa ctctttgctg tagacttgat
    42841 cggcatagag ggcagcacaa ggttgcgccc atgccacacc actcggcatg ggggtccgct
    42901 ccgggcgatt cgagaagttg aggttatgcg agtccaggga agcctaaagc atcaaagtag
    42961 tcgactgtaa gtatacggtt cagcccagtt tagctgtgtc tccaccagtg ccccgcgcct
    43021 cctgccataa gtgcggcttg ctcatggata tccccgagac ggcttacggc gcaccggata
    43081 ataaatttcg cgtatacatg gtattcctta ccggagaaat tataagccca acaacaatac
    43141 gcctgtgaca ttgttcgctc aaacttatcg cgagtgattt ggctcacact ccggtaaaga
    43201 ttccccatca cgccaacggc acataaacat ccgtttccag tcgctgctct gtcacccctt
    43261 cggcaagatt ggtgtagtga aacagaatcg gtgctttacg cattttctcg ccgcttgcag
    43321 gcagccagtg gcgaataatg ccccatatcg tgtgtgaaat atcgtccagc tcgccaacgt
    43381 ggcgggccac ggcataacgt ccaccggtaa gttcaccatt gctcacacca taacgattat
    43441 cgggaatcgg ttcgctaacg ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg
    43501 tggcaggggc gtcccaggcg acgccaaaag tctggctttg gttaacagga gaaagccccg
    43561 tctctttacg ccacatgata aatttcgcca cactgtagtt gagcaactca gggctacagc
    43621 ggtgcggcaa catcgccaca gggattgatg ggaaatcgat aatgtttaca tccagtgtca
    43681 ggtttgtcat tttgcctccc tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac
    43741 aattacggat ttactttgcg gatctgtttg acgtcaattt caacagaatt ccagtcttta
    43801 tcgacttcac cctgaatctc aaccgtatct ttcggcgtca ccgtcacacc gttccagcgt
    43861 ttgtgatcga tatcaacatt gatagtaccg ctggcatctt taaacacgta gagatcgtca
    43921 gagatgcgtt caacgatatt gccgcgcagg gttacccagg tgtcgtcacg cagggatttt
    43981 gcgctttcta cagtcgttac gctgccgttc ggcccctgaa atcctccggc ctgactttgc
    44041 gttaccgatg ggccagaaaa accgccctgc tctgctgcca tcaccggtgc actgcacagg
    44101 gccattactg cgattactgc tgcgaatttt ttcatgttta ttactccctt taatgtctgt
    44161 ttccgagcat ttaacaagat agtccttaac aacttcttaa gggaaaaaaa taaaatttaa
    44221 tgctgtacag agcgcgttac aacacggttt actggcagca aatacggtta tcgcagggat
    44281 gaaaaaatgc gaattttact gatagaagat gacatgctga ttggtgacgg catcaaaact
    44341 ggccttagta aaatgggttt tagtgtcgac tggtttacac aaggtcgtca gggaaaagag
    44401 gcgctttata gcgcacctta tgatgcggtg atcctggatt taaccttacc aggcatggat
    44461 ggtcgcgata ttttgcgcga atggcgagag aaaggtcagc gtgagccggt actgaacctg
    44521 accgcgcgtg atgcgttagc ggaacgtgta gaagggctgc gtctgggagc tgacgattat
    44581 ctgtgtaaac cttttgcgtt gatagaagtc gccgccaggc tggaggctct gatgcgccga
    44641 accaacggcc aggccagcaa cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa
    44701 cgtatcgcca cgctggctgg cgaaccctta acactgaaac caaaagaatt tgccctgctg
    44761 gaattactga tgcgtaacgc tggtcgggta ctgccgcgca aactgattga agagaaactg
    44821 tatacctggg acgaagaggt caccagtaat gccgttgaag tgcatgtgca tcatctgcga
    44881 cgcaaactcg gtagtgattt tattcgtacc gtgcatggta ttggctacac attaggtgag
    44941 aaatgaaatt tacccaacgt cttagtctgc gcgtcaggct gacgctaatc tttttaattc
    45001 tggcctcggt gacctggctg ctttccagct ttgtcgcctg gaaacaaaca acggataacg
    45061 tcgatgaatt gttcgacacc caactgatgc tgtttgccaa acggttaagt acgctcgatc
    45121 tcaacgaaat caacgcggcg gatcgcatgg cacagacgcc aaataaatta aaacacggtc
    45181 atgttgatga cgatgcgctg acctttgcca tctttaccca cgacggcaga atggtcctta
    45241 atgatggcga taacggagaa gatattccct atagctatca acgggaaggt tttgctgacg
    45301 ggcaactggt cggtgaagac gatccttggc gttttgtctg gatgacctca cctgatggca
    45361 aatatcgcat cgttgttggc caggaatggg aataccgtga agacatggcg ctggcgattg
    45421 ttgccgggca attgatcccg tggctggtcg cactgccgat tatgttaatc atcatgatgg
    45481 tactactggg tcgtgaactc gcgccgctga acaaactggc gctggcacta cgtatgcgtg
    45541 accctgactc ggaaaaacca ctaaacgcga ctggcgtacc cagcgaagtg cggccactgg
    45601 ttgagtcgct aaatcaactg ttcgcccgca cacatgcgat gatgattcga gaacgacgct
    45661 ttacctccga tgcagctcac gaactacgta gcccgttaac agcgctgaaa gtgcaaaccg
    45721 aagttgcgca gctctctgac gatgatccgc aggcgcggaa aaaagcactg ctccaattac
    45781 attccg
//
LOCUS       DAJVPP010000021        45346 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVPP010000021 DAJVPP010000000
VERSION     DAJVPP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.021"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            114..2267
                     /locus_tag="OQM34_001156"
     CDS             114..2267
                     /locus_tag="OQM34_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1451687.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="OQM34_001157"
     CDS             2320..4050
                     /locus_tag="OQM34_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451688.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OQM34_001158"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OQM34_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1451689.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OQM34_001159"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OQM34_001159"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1451690.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OQM34_001160"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OQM34_001160"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1451691.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OQM34_001161"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OQM34_001161"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1451692.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OQM34_001162"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OQM34_001162"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1451693.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OQM34_001163"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OQM34_001163"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1451694.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OQM34_001164"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OQM34_001164"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1451695.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OQM34_001165"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OQM34_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1451696.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OQM34_001166"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OQM34_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1451697.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OQM34_001167"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OQM34_001167"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1451698.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OQM34_001168"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OQM34_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1451699.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OQM34_001169"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OQM34_001169"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1451700.1"
                     /translation="MNIIEANIATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OQM34_001170"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OQM34_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1451701.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OQM34_001171"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OQM34_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1451702.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="OQM34_001172"
     CDS             16308..16847
                     /locus_tag="OQM34_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1451703.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="OQM34_001173"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="OQM34_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1451704.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="OQM34_001174"
     CDS             complement(18206..18553)
                     /locus_tag="OQM34_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1451705.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OQM34_001175"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OQM34_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1451706.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEEPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OQM34_001176"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OQM34_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1451707.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OQM34_001177"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OQM34_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1451708.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OQM34_001178"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OQM34_001178"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1451709.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OQM34_001179"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OQM34_001179"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1451710.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="OQM34_001180"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="OQM34_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OQM34_001181"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OQM34_001181"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCS1451711.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OQM34_001182"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OQM34_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1451712.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OQM34_001183"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OQM34_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1451713.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OQM34_001184"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OQM34_001184"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1451714.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OQM34_001185"
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OQM34_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS1451715.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="OQM34_001186"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="OQM34_001186"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1451716.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSCEQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="OQM34_001187"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="OQM34_001187"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1451717.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEKLTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="OQM34_001188"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="OQM34_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1451718.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OQM34_001189"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OQM34_001189"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1451719.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="OQM34_001190"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="OQM34_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1451720.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="OQM34_001191"
     CDS             complement(40011..40466)
                     /locus_tag="OQM34_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451721.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="OQM34_001192"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="OQM34_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OQM34_001193"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OQM34_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1451722.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="OQM34_001194"
     CDS             complement(41309..41986)
                     /locus_tag="OQM34_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1451723.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDYQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OQM34_001195"
     CDS             complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OQM34_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1451724.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OQM34_001196"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OQM34_001196"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1451725.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="OQM34_001197"
     CDS             complement(43192..43650)
                     /locus_tag="OQM34_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1451726.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="OQM34_001198"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="OQM34_001198"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1451727.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..>45346)
                     /gene="adrA"
                     /locus_tag="OQM34_001199"
                     /gene_synonym="dgcC"
     CDS             complement(44596..>45346)
                     /gene="adrA"
                     /locus_tag="OQM34_001199"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1451728.1"
                     /translation="STAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVS
                     FNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHW
                     ETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRGS
                     DVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVGVAPLNP
                     QMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11796 a        11813 c        11089 g        10648 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgtc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttg cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa tgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caattttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gttcttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccagg gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtc gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc cagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat caggtggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtt gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagaact ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgagaag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgcccta gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atggtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtgccg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccaa gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca agcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttgtt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgataa tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactat ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gcgatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaa
//
LOCUS       DAJVPP010000022        44331 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVPP010000022 DAJVPP010000000
VERSION     DAJVPP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.022"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..468
                     /gene="satP"
                     /locus_tag="OQM34_001200"
     CDS             <1..468
                     /gene="satP"
                     /locus_tag="OQM34_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1451729.1"
                     /translation="LDGIILAMGIFYGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWL
                     TLVAILLMPKLGLTDAPNAQFLGVYLGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLF
                     ALLAIGNIAGNAAIIHFAGWIGLICGASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(503..1090)
                     /gene="mog"
                     /locus_tag="OQM34_001201"
     CDS             complement(503..1090)
                     /gene="mog"
                     /locus_tag="OQM34_001201"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1451730.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(1205..2158)
                     /gene="tal"
                     /locus_tag="OQM34_001202"
     CDS             complement(1205..2158)
                     /gene="tal"
                     /locus_tag="OQM34_001202"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1451731.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2437..3867
                     /locus_tag="OQM34_001203"
                     /pseudo
     CDS             2437..3867
                     /locus_tag="OQM34_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3937..4713
                     /gene="yaaA"
                     /locus_tag="OQM34_001204"
     CDS             3937..4713
                     /gene="yaaA"
                     /locus_tag="OQM34_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1451732.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4866..5162)
                     /locus_tag="OQM34_001205"
     CDS             complement(4866..5162)
                     /locus_tag="OQM34_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1451733.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(5376..6662)
                     /gene="thrC"
                     /locus_tag="OQM34_001206"
     CDS             complement(5376..6662)
                     /gene="thrC"
                     /locus_tag="OQM34_001206"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1451734.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6663..7595)
                     /gene="thrB"
                     /locus_tag="OQM34_001207"
     CDS             complement(6663..7595)
                     /gene="thrB"
                     /locus_tag="OQM34_001207"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1451735.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7597..10059)
                     /gene="thrA"
                     /locus_tag="OQM34_001208"
     CDS             complement(7597..10059)
                     /gene="thrA"
                     /locus_tag="OQM34_001208"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1451736.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(10085..10204)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(10140..10205)
                     /gene="thrL"
                     /locus_tag="OQM34_001209"
     CDS             complement(10140..10205)
                     /gene="thrL"
                     /locus_tag="OQM34_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1451737.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(10419..11105)
                     /locus_tag="OQM34_001210"
     CDS             complement(10419..11105)
                     /locus_tag="OQM34_001210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1451738.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11505..11645)
                     /locus_tag="OQM34_001211"
     CDS             complement(11505..11645)
                     /locus_tag="OQM34_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451739.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDSTVSKTAVILTILT
                     AT"
     gene            11741..12457
                     /gene="arcA"
                     /locus_tag="OQM34_001212"
     CDS             11741..12457
                     /gene="arcA"
                     /locus_tag="OQM34_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1451740.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12516..13868)
                     /gene="creD"
                     /locus_tag="OQM34_001213"
     CDS             complement(12516..13868)
                     /gene="creD"
                     /locus_tag="OQM34_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1451741.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13926..15350)
                     /gene="creC"
                     /locus_tag="OQM34_001214"
     CDS             complement(13926..15350)
                     /gene="creC"
                     /locus_tag="OQM34_001214"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1451742.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVSLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(15350..16039)
                     /gene="creB"
                     /locus_tag="OQM34_001215"
     CDS             complement(15350..16039)
                     /gene="creB"
                     /locus_tag="OQM34_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1451743.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(16052..16525)
                     /gene="creA"
                     /locus_tag="OQM34_001216"
     CDS             complement(16052..16525)
                     /gene="creA"
                     /locus_tag="OQM34_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1451744.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16736..17605
                     /gene="robA"
                     /locus_tag="OQM34_001217"
     CDS             16736..17605
                     /gene="robA"
                     /locus_tag="OQM34_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1451745.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17602..18249)
                     /gene="gpmB"
                     /locus_tag="OQM34_001218"
     CDS             complement(17602..18249)
                     /gene="gpmB"
                     /locus_tag="OQM34_001218"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1451746.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            18301..18821
                     /gene="yjjX"
                     /locus_tag="OQM34_001219"
                     /pseudo
     CDS             18301..18821
                     /gene="yjjX"
                     /locus_tag="OQM34_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18906..19232)
                     /gene="trpR"
                     /locus_tag="OQM34_001220"
     CDS             complement(18906..19232)
                     /gene="trpR"
                     /locus_tag="OQM34_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1451747.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(19322..21259)
                     /gene="sltY"
                     /locus_tag="OQM34_001221"
     CDS             complement(19322..21259)
                     /gene="sltY"
                     /locus_tag="OQM34_001221"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1451748.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGHSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            21470..23137
                     /gene="ettA"
                     /locus_tag="OQM34_001222"
     CDS             21470..23137
                     /gene="ettA"
                     /locus_tag="OQM34_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1451749.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(23244..23411)
                     /locus_tag="OQM34_001223"
     CDS             complement(23244..23411)
                     /locus_tag="OQM34_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451750.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(23445..24677)
                     /gene="nadR"
                     /locus_tag="OQM34_001224"
     CDS             complement(23445..24677)
                     /gene="nadR"
                     /locus_tag="OQM34_001224"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1451751.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24698..26080)
                     /gene="radA"
                     /locus_tag="OQM34_001225"
     CDS             complement(24698..26080)
                     /gene="radA"
                     /locus_tag="OQM34_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1451752.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(26129..27097)
                     /gene="serB"
                     /locus_tag="OQM34_001226"
     CDS             complement(26129..27097)
                     /gene="serB"
                     /locus_tag="OQM34_001226"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1451753.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            27203..27847
                     /locus_tag="OQM34_001227"
     CDS             27203..27847
                     /locus_tag="OQM34_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1451754.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27875..28890
                     /gene="lplA"
                     /locus_tag="OQM34_001228"
                     /pseudo
     CDS             27875..28890
                     /gene="lplA"
                     /locus_tag="OQM34_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28922..29185
                     /locus_tag="OQM34_001229"
     CDS             28922..29185
                     /locus_tag="OQM34_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1451755.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(29346..30065)
                     /gene="deoD"
                     /locus_tag="OQM34_001230"
     CDS             complement(29346..30065)
                     /gene="deoD"
                     /locus_tag="OQM34_001230"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1451756.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(30122..31345)
                     /gene="deoB"
                     /locus_tag="OQM34_001231"
     CDS             complement(30122..31345)
                     /gene="deoB"
                     /locus_tag="OQM34_001231"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1451757.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(31397..32719)
                     /gene="deoA"
                     /locus_tag="OQM34_001232"
     CDS             complement(31397..32719)
                     /gene="deoA"
                     /locus_tag="OQM34_001232"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1451758.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32846..33625)
                     /gene="deoC"
                     /locus_tag="OQM34_001233"
     CDS             complement(32846..33625)
                     /gene="deoC"
                     /locus_tag="OQM34_001233"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1451759.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33883..35424
                     /locus_tag="OQM34_001234"
     CDS             33883..35424
                     /locus_tag="OQM34_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1451760.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            35396..36259
                     /locus_tag="OQM34_001235"
     CDS             35396..36259
                     /locus_tag="OQM34_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1451761.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(36473..37255)
                     /locus_tag="OQM34_001236"
     CDS             complement(36473..37255)
                     /locus_tag="OQM34_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1451762.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAIHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(37252..38325)
                     /locus_tag="OQM34_001237"
     CDS             complement(37252..38325)
                     /locus_tag="OQM34_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1451763.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(38447..38608)
                     /locus_tag="OQM34_001238"
     CDS             complement(38447..38608)
                     /locus_tag="OQM34_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1451764.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38725..39330)
                     /gene="osmY"
                     /locus_tag="OQM34_001239"
     CDS             complement(38725..39330)
                     /gene="osmY"
                     /locus_tag="OQM34_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCS1451765.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39723..41312)
                     /gene="prfC"
                     /locus_tag="OQM34_001240"
     CDS             complement(39723..41312)
                     /gene="prfC"
                     /locus_tag="OQM34_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1451766.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(41403..42080)
                     /gene="yjjG"
                     /locus_tag="OQM34_001241"
     CDS             complement(41403..42080)
                     /gene="yjjG"
                     /locus_tag="OQM34_001241"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1451767.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(42095..42541)
                     /gene="rimI"
                     /locus_tag="OQM34_001242"
     CDS             complement(42095..42541)
                     /gene="rimI"
                     /locus_tag="OQM34_001242"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1451768.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42510..42923)
                     /gene="holD"
                     /locus_tag="OQM34_001243"
     CDS             complement(42510..42923)
                     /gene="holD"
                     /locus_tag="OQM34_001243"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1451769.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            43026..44057
                     /gene="rsmC"
                     /locus_tag="OQM34_001244"
     CDS             43026..44057
                     /gene="rsmC"
                     /locus_tag="OQM34_001244"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1451770.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        10404 a        12114 c        11228 g        10585 t
ORIGIN      
        1 ctggacggta ttattcttgc catgggcatt ttctacggcg gcatcgcgca aatttttgct
       61 ggtctgctgg agtacaaaaa aggcaacact ttcggtttaa ccgcattcac ctcttacggt
      121 tctttctggc tgacgctggt tgcgattctg ctgatgccga aactgggtct gaccgatgcg
      181 ccaaatgcac agttccttgg tgtctacctg ggtctgtggg gcgtatttac gctgtttatg
      241 ttcttcggca ctctgaaagg cgcgcgcgtc ctgcaattcg ttttctttag cctgaccgtg
      301 ctgtttgccc tgctggcgat cggtaacatt gccggtaatg ccgcgatcat ccactttgcc
      361 ggctggattg gtctgatctg cggtgccagc gcaatctatc tggcgatggg tgaagtgctg
      421 aacgagcagt ttggtcgcac cgttctgccg attggtgaat cccactaatt gtcttgagat
      481 ccccccgctc ggggggattt ttttattcgc taacgtcgcg tcttgcactc ttcggtctga
      541 atgcagcaac cacttctggt gccgtttcaa cgtatggccc ttccagcaac tgaatgcagt
      601 acggtacgct ggcaaaaata ccatgcacca caacgttacc ctcagcgtcc ttcacacctt
      661 ccagcgtctc tttaatagac ttcggctgac cgggtaagtt aaggatcagc gcctgtttgc
      721 gaatcacgcc cacctgacgc gaaaggatcg cagttggtac aaaatgcagg ctgatctggc
      781 gcatctgttc accaaagcca ggcatctcgc ggtccgctac tgccagcgtc gcatcgggcg
      841 ttacgtcacg acgcgccggg ccagttccgc ccgtggtgag caccagatgg caactcattt
      901 catccaccag ctcacacaac gtttgctcga tgatcgcctg ctcatcgggg attaagcggg
      961 tttccagttc aaacggcgtg gttagcgccg atgtcagcca ttcttccagc gctggaatgc
     1021 ctttatcctg ataaacgccg ctggatgcgc gatcagagat ggaaactaag ccaatacgta
     1081 aagtattcat caagattttt ccgtttaaac aatctcgtta aacagaatga tacactgcga
     1141 agggagtgac agacaggctt cagaagaggt agcgtgaccg acttcccggt cacgctaaga
     1201 atgattacag cagatcgccg atcatttttt ccagtttttc ctggtcaata gcaaacttac
     1261 ggataccttc cgccagttta tctactgcca ttggatcctg gttgtgctgc cacaggaact
     1321 cggactcagt gatacgcgcc ggacgcgctt tcacttcgcc ggtgtaagac agtttacgtt
     1381 cgatagcccc ttcgctctcc gccagctctt tcagcagtgc cggtgcgatg gtcagacggt
     1441 cacagcctgc cagttccaga atttcgccga tgttacggaa gcttgcgccc ataaccacgg
     1501 tttcataacc gtgctctttg tagtactggt agatttcaga tacagaaacc acgcccggat
     1561 cttctgccgg agcgtactct ttcttatcgg tattcgcttt gtaccagtca agaatacggc
     1621 caacaaacgg cgagatcagg aacacgcccg cctccgcaca agcacgagcc tgagcgaagg
     1681 agaacagcag ggtcaggtta cagttgatac cttctttttc cagctgttct gcagcacgga
     1741 taccctgcca ggtagaagcc agtttgatca gaatacgatc gttgctaata cctgcatcgt
     1801 tgtagagttt gatcaggcgt tttgctttcg caattgacgc ttcggtgtca taggaaagac
     1861 gcgcatcaac ttcagttgag atacggcccg gaaccagttt caggatttcc agaccaatat
     1921 ttactgccag tttgtcggtc gcgtccacga tctgctgcgc gcgatcgttg ctctgctgtt
     1981 tcgcccaggc aacagcatca tcaatcaact tacggtattc cggaatctgc gctgcgttaa
     2041 gaatgagaga agggttggtt gtggcatcct gcggttgata cagcttcatt gccgcgatgt
     2101 ccccagtgtc ggccactacg gtggtgtact gacgaaggga ggtcaatttg tccgtcatga
     2161 tagtatttct ctttaaacag cttgttaggg ggatgtaacc ggtctgccct gatgatatca
     2221 cgacgcaacg tcagcgcaac cgccgacaag acggttatcg ccaggcgaga ctgtttcgga
     2281 tttctgacac tgcgttgata tcgcccacca tttttataca aaacctcatg tatgctacgc
     2341 agaagttatc aagtacctcg tagcgtatat acttcttaaa caattggtaa cgtttacaca
     2401 ggaaagtcat cgcgaccggc aataagaggg atatgcatgc cagatttttt ctcctttatt
     2461 aacagcgtcc tttggggatc ggtaatgatt tacctgctct tcggcgcagg ttgttggttc
     2521 acttttcgca ccggatttgt gcagtttcgc tacatccgcc agtttggcaa aagtcttaaa
     2581 aatagcattc atccacagcc aggcggttta acctcatttc agtcattgtg taccagtctt
     2641 gcggcgcgcg tgggtagcgg caacctggcc ggcgttgcgc tggcaattac cgccggtgga
     2701 ccaggtgccg tcttttggat gtgggttgct gcgtttatcg gcatggcgac ctcgtttgcc
     2761 gaatgttccc ttgcacaact ttataaagaa cgtgacgtca atgggcagtt tcgtggcgga
     2821 ccggcatggt atatggcgcg cgggctgggg atgcgctgga tgggcgttct gttcgccgtc
     2881 tttttgctca tcgcctacgg cataattttc agcggagttc aggcgaacgc cgttgcccga
     2941 gccctgagtt tttcttttga ttttcctccg ctggtgacgg gcattattct cgctgtcttt
     3001 gctctgctgg caatcactcg cggtcttcat ggcgtcgccc ggctcatgca ggggtttgtc
     3061 ccgttgatgg cgataatctg ggtactgacc agcctggtga tttgcgtaat aaatatcggg
     3121 caacttcccc acgtcatttg gtctattttt gagagtgctt ttggctggca ggaagcggca
     3181 ggcggcgcgg cgggatatac cttaagccag gcgattacta acggttttca gcgcagtatg
     3241 ttttccaatg aggtgggaat gggatcgacg ccaaacgcgg cagcggcagc tgcatcctgg
     3301 cctccgcatc cggcagcaca agggattgtc cagatgattg gcatttttat cgacaccctg
     3361 gtcatctgta cggcaagcgc catgctgata ttactggcgg gtaacggcac aacctacatg
     3421 ccgctggaag gtattcagct tatccagaag gcgatgcggg tgctaatggg ttcctagggt
     3481 gctgaatttg ttaccctcgt ggttattctg tttgccttca gctccatcgt tgccaactac
     3541 atttatgccg aaaacaatct cttcttttta cgcctgaaca accctaaagc gatctggtgt
     3601 ttgcggatct gcaccttcgc aacggtcatc ggcggcacct tgctaagcct tccgctgatg
     3661 tggcaactgg cagatatcat aatggcctgc atggctatta ccaatttgac cgccatttta
     3721 ctgctctcgc ctgtggttca taccattgcc agtgattatc tacgccagcg taaactcggc
     3781 gtgcgcccgg tgtttgatcc gttgcgttat ccggatatcg gtcgccagct ttctccggac
     3841 gcgtgggatg atgtttcgca ggagtaatca caactatcga tcaactcatt ctcttttttt
     3901 gctaaagtcg gcataaattt cctgcaagga ctggatatgc tgattcttat ttcacctgcg
     3961 aaaacgcttg attaccaaag cccgttgacc accacgcgct atacgctgcc ggagctgtta
     4021 gacaattccc agcagttgat ccatgaagcg cggaaactga cgcctccgca gattagcacg
     4081 ctgatgcgca tcagcgacaa actggcgggt atcaacgccg ctcgctttca tgactggcag
     4141 ccagatttca cgccggcgaa tgcccgccag gcgattctgg cgtttaaagg tgatgtctac
     4201 accggcttgc aggccgaaac cttcagcgaa gacgatttcg attttgccca acagcatttg
     4261 cgaatgcttt ccggcttgta tggcgtactg cgcccgctcg atttaatgca gccttatcgt
     4321 ctggaaatgg ggatccgtct tgagaatgcc cgagggaaag atctgtatca attctgggga
     4381 gatatcatca ccaacaagct gaacgaggcg ctcgcagcac aaggcgataa tgtggtgatt
     4441 aatctggcgt cagatgagta ttttaaatcg gtgaagccga agaaattgaa tgccgagatt
     4501 atcaagccgg tgttcctcga tgagaagaac ggcaaattta agatcatcag cttctacgct
     4561 aagaaagcac gcggtctgat gagtcgtttc attattgaaa atcggctgac caaaccagag
     4621 caactgactg gttttaatag cgaaggttac ttctttgatg aagattcctc cagcaatggc
     4681 gaactggtgt ttaaacgcta cgagcagcgt taagtttgta gtcaatgccg gatgcggcgt
     4741 gaacgcctta tccggcctac aaaagcatgc agattcaata tattgcagaa ttaacgtagg
     4801 cctgataaga cgcgccagcg tcgcatcagg cgctgaatgc cggattgtta cccggcattt
     4861 gtcatttagc ggtgatgttt gcctggacca tgaccgccgt gatgatcatg aggagctttc
     4921 ttatggtggc gcggcggtgg cggcggtccg tgtgggtgcc agcgattgcc tcgccattca
     4981 taatgttgtt tccaccagcc gtggtcgcgc cagtgacctc catcccagta atagccacga
     5041 ttatcacgat cgcctatctg taattttact gacgggacta acgtaatttc cgcagcctgt
     5101 gctgccatgg gagcgaccag aaccagggaa agtgcgagta cgatagattg catctttttc
     5161 acaggttact ccttttatca ttgcccgtta tgggtcgata tccggagatt acgcgatgag
     5221 aacgacggtt gttattctcc gcaatcctaa tctctaagtt accgcattta ttgataattt
     5281 ctcctttttc cctgtcgttt ttctccataa tttcttcata aaaaagccgg gctgcataaa
     5341 agcaaacccg gcctgattga gataatgaat agattttact gatgattcat catcaattta
     5401 cgcaacgcag aaaaatcggc gggcagatta tgtgaaagca agggtaaatc agcacgttct
     5461 gccagctctt ttggcagatc caacgtttca ccgagaatcg cttccacgct ctctttaaat
     5521 ttcgccggat gcgcggtgcc gaggaacaag ccatattcgc ctggattcaa ctggtcacgc
     5581 agcgcacgat acgctacggc agcgtgcggc tccgaggtgt agcccagttc ttttaactca
     5641 cgcattgtct gttgcgtggt ttcatcatcc acggctgcat aacccagctc tttcagttgc
     5701 cagattttgc ggcggaacaa ctcttccaca cgcggccagt tgttcggctg actcacgtcc
     5761 atcgcgttgg ataacgtcgc ctgagtcgct ttgggtgacc actgaccgtc gtgcaggaaa
     5821 cgtggcacgg tatcgttcac gttggtcgca gcaataaaac gtttcaccgg cagaccgaat
     5881 gacttcgcca gcagacccgc cgtcaaatcg ccgaagtttc cgcttggcac cgagacaacc
     5941 agctggttgc gcgcctcctg cggcagctgc gcaacagctt caaagtagta gcaaatctgc
     6001 gccagcaaac ggctgatgtt aatcgagtta gccgagttta accccagcgc cactttcagt
     6061 tcttcatcat caaacgcctg cttcaccagc gcctgacagg catcgaaatc gccgtcgatg
     6121 gcaacagttt cgatattgcc gcccaacgta cagaacagtt tttcttgcag tggactgatt
     6181 ttgcctcgtg gatagaggat aaccactttc acattcggca aaccgtagaa agcatgagcc
     6241 actgcagctc cggtatcacc ggaggtcgcg gtcagaatgg tcactggctt atcatccgca
     6301 atatgggtca gcatttgtgc cataaagcga ccgccgaaat ctttaaatgc cagcgttggc
     6361 ccgtggaaca attccagaca accgacatcg ctttcaacat tggcgaccgg agccgggaag
     6421 gcaaacgccg cgcgcacgcg ctcttccagg atttcctgtg ggatttcatc accaataaac
     6481 gccgagagga tcttcgcact gcgggtgaca aaatccagct tcagcatctc atcaatttca
     6541 gtcaggctga attccggcag gtcgtgcgga aaaaacagcc cctgattttt gcccaacccc
     6601 tgggttacgg cttgcgcaaa gctgacctgc tcgttgtgat ctttcagatt gtagagtttc
     6661 atttagtttt ccagtactcg tgcgcccgcc gtatccagcc ggcaaatatg aacaaaacct
     6721 tcctgatttt gcaggtagtt cttacccaac cagtcggcaa cgcgctgggc ggtatccggc
     6781 ttgtcacata gagcgaacaa ggtcgggccg gagccggaga taccgctcac taccgcgccg
     6841 atttccgcga ccgcctgccg cgcctgccgg aagccaggca gtaaccgttc acggtagggt
     6901 tcagcgataa catctttcat cagcttcgcg gcaagctcag gctgacggga atagcaggcg
     6961 tgaatgaagc cagccagatg tcgcccgtgc gcaatgcaat cctggcggcg atactgcgcc
     7021 ggtaaaatag cccgggcttc tgccgtcgag actttaattc ccggatacgc cagcacccac
     7081 agccactcat caaaccctgg cacttgctgg ctgatgatgt cgttttcttc gatcatcaac
     7141 tgcataccac caagaaaaca cggtgccacg ttgtcgtaat gaatgctgcc ggagatacgt
     7201 ccttccagct cgcccatcaa agccagcaaa cgagtgtcat taagcggctt gccgcagtgt
     7261 tcattcatcg ccatcagcgc cgcgaccacc gaacaggcgc tggagcctaa gcccgaaccg
     7321 atcggcatat tcttttccag agtcatcgcc actggaattt gcttgccaag ctcctggcaa
     7381 aaacgctccc agcactgata aacgatattt tcccgtggtt ctgacggcag cttatcggca
     7441 aagcgtccga ggttgttgag actgaatgtc tctgccgcct caaccgtgac tacatctccg
     7501 agcaatgcac catcaacggg tgccaccgcc gccccgagca catcaaaccc gacgctcata
     7561 ttggcactgg aagccggggc ataaacttta accatgtcag actcctaact tccatgagag
     7621 ggtacgtagc agatcggcaa agacaccggc agcggtaacg tcattgcccg caccatatcc
     7681 gcgcagcacc aacggcagcg gctgataata gtggctataa aaggccaggg cgttttcgcc
     7741 atttttcact ttgaacagcg gatcattccc atccacttcg gcaatcttca cgcggcagac
     7801 accatcttca tcaatattgc caacatagcg caaaactttt ccttcatcac gggccttcgc
     7861 cacgcgcgcg gcaaagagat cgtcgagctg tgacagattc gccataaaag cggcaacatc
     7921 accctcggcg ttaaactctg cgggcagcac aggttcaatt tcaatatccg ccagctccag
     7981 ttcacgtccc gtttcacgag cgagaatcaa tagtttacgc gccacatcca taccagaaag
     8041 atcatctcgc gggtccggtt cggtataacc catttcccgc gccagcgtgg tcgcctcgga
     8101 gaaactcatg ccttcgtcta acttgccgaa gatataagaa agcgaacctg aaagaatgcc
     8161 ggagaacttc accaattcat cacctgcatt gagcagattt tgcaggttct caataaccgg
     8221 taatccagcc ccaacgttgg tgtcatagag gaatttacgc cgcgattttt ccgccgcata
     8281 acgcaactga tgatagtaat ccatcgacga ggtgttggcc tttttgttcg gcgtgacaac
     8341 gtggaaacct tcgcgcagga agtcggcata ttgatccgcc actgcctggc tggaggtgca
     8401 gtcaacaatg accgggttca gcagatgata ttctttcacg aggcgaatta agcgcccgag
     8461 attaaacggc tctttggctt gtgcaagttc ttcctgccag ttttccagat taaggccatg
     8521 cacactagtg agcagagcct tcgagttggc aacgccgcag acacgtaagt cgatatgttt
     8581 attcttcagc cagctttgct gacgcttcag ttgctccagc agcgcaccgc caacgccacc
     8641 gacgccaatc acaaacactt cgataacctg atcggtattg aacagcatct gatgagtaac
     8701 gcgcacgcca gtggtcgcat catcgttatt taccacgaca gagattgagc gttcagaaga
     8761 tccctgagca atggcgacaa tgttgatatt ggcgcgggcc agcgcggcaa agaatttcgc
     8821 cgagatccca cgcaaggtgc gcataccatc acctaccacc gagataatgg ccagccgttc
     8881 cgtcaccgcc agcggctcca gtaagccttc tttcagttcc aggtagaact cttcctgcat
     8941 tgcccgttca gctcgcacac agtcgctttg tggaacgcag aaactgatac tgtattcgga
     9001 agatgattgc gtaatcagca ccacggaaat acgggcgcgt gacattgcag caaagacgcg
     9061 cgccgccatg ccaaccatcc ctttcatccc cgggccggaa acgctgaaca ttgccatgtt
     9121 attcagattg gaaatgccct tgaccggtaa ttcgtcttca tcacggctgg caccaatgag
     9181 cgtacctggt gcttgaggat ttccggtatt tttaatcagg caagggatct ggaactgggc
     9241 gatgggggta atggtgcggg ggtgaagaac tttagcgccg aagtaggaaa gctccatcgc
     9301 ttcctggtag gacatcgact tcaacaacct cgcatcgggc acctgacgcg ggtcgcaggt
     9361 atagaccccg tcaacgtccg tccaaatctc gcaacaatcg gcgcgtaaac aggcagccag
     9421 caccgccgcg gagtagtcgg aaccgttacg tccaagcacc accagttcgc ctttttcatt
     9481 accggcggtg aaacctgcca tcaacaccat gtgatcagcc ggaatgcggc ttgccgcaat
     9541 acggcgggtg gactcagcaa tatcgacggt agattcgagg taatgcccca ctgccagcag
     9601 tttttcgacc ggatcgataa cggtaacgtt gtgaccacgc gcttctaaca cgccggccat
     9661 aatggcgatc gacattttct cgccacggca aatcagcgca gcgttgatgc tatccgggca
     9721 ctgccccaac aaactaatgc catgcaggac atgttttatt tgggcaaatt cctgatcgac
     9781 gaaagttttc aattgcgcca gcgggaaccc cggctgggcg gcggcgagtc ccgtcaaaag
     9841 ttcggcaaaa atacgttcgg catcgctgat attgggtaaa gcatcctggc cgctaatggt
     9901 tttttcaatc atcgccacca ggtggttggt gattttggcg ggggcagaga ggacggtggc
     9961 cacctgcccc tgcctggcat tgctttccag aatatcggca acacgcagaa aacgttctgc
    10021 atttgccact gatgtaccgc cgaacttcaa cactcgcatg gttgttacct cgttaccttt
    10081 ggtcgaaaaa aaagcccgca ctgtcaggtg cgggcttttt tctgtgtttc ctgtacgcgt
    10141 cagcccgcac cgttacctgt ggtaatggtg atggtggtgg taatggtggt gctaatgcgt
    10201 ttcatggatg ttgtgtactc tgtaattttt atctgtctgt gcgctatgcc tatattggtt
    10261 aaagtattta gtgacctaag tcaataaaat tttaatttac tcacggcagg taaccagttc
    10321 agaagctgct atcagacact ctttttttaa tccacacaga gacatattgc ccgttgcagt
    10381 cagaatgaaa agctgaaaaa tacttactaa ggcgtttttt atttggtgat atttttttca
    10441 atatcatgca gcaaacggtg caacattgcc gtgtctcgtt gctctaaaag ccccaggcgt
    10501 tgctgtaacc agtcgaccag ttttatgtca tctgccactg ccagagtcgt cagcaatgcc
    10561 atgactcgtt cgcgtaaagc ttgcagttga tgttggtctg ccgttgtgtc acttttcgcc
    10621 ggttgttgta ttaatgttgc taattgatag caatagacca tcaccgcctg ccccagattg
    10681 agcgaaggat aatccgccac catcggcaca ccagtaagca cgtcagccaa cgctaactct
    10741 tcgttagtca gcccggaatc ttcgcgacca aacaccagcg cggcatggct catccatgaa
    10801 gatttttcct ctaacagcgg caccagttct actggcgtgg cgtagtaatg atatttcgcc
    10861 cgactacgcg cagtggtggc gacagtgaaa tcgacatcgt gtaacgattc agccagtgtc
    10921 gggaaaactt taatattatc aataatatca ccagatccat gtgcgaccca gcgggtggct
    10981 ggctccaggt gtgcctgact atcgacaatc cgcagttcgc taaaacccat cgttttcatt
    11041 gcccgcgccg ctgccccaat attttctgct ctggcgggtg cgaccaggat aatcgttata
    11101 cgcatattgc cactcttctt gaccaaataa ccgcgaacag ggtgatcact gtcaacttat
    11161 tacgcggtgc gaatttacaa attcttaacg taagccgcag aaaaagccct ttacttagct
    11221 taaaaaaggc taaactattt tctgactgca ctaacggttt aattgttaaa aaatgctaca
    11281 tatccttctg tttacttagg ataattttat aaaaaataaa tctcgacaat tggattcacc
    11341 acgtttatta gttgtatgat gcaactagtt ggattattaa aataatgtga cgaaagctag
    11401 catttagata cgatgatttc atcaaactgt taacgtgcta caattgaact tgatatatgt
    11461 caacgaagcg tagttttatt gggtgtccgg cccctcttag cctgttatgt tgctgttaaa
    11521 atggttagga tgacagccgt ttttgacact gtcgagtcct gagggaaagt acccacgacc
    11581 aagctaatga tgttgttgac gttgatggaa agtgcatcaa gaacgcaatt acgtacttta
    11641 gtcatgttac gccgatcatg ttaatttgca gcatgcatca ggcaggtcag ggacttttgt
    11701 acttcctgtt tcgatttagt tggcaattta ggtagcaaac atgcagaccc cgcacattct
    11761 tatcgttgaa gacgagttgg taacacgcaa cacgttgaaa agtattttcg aagcggaagg
    11821 ctatgatgtt ttcgaagcga cagatggcgc ggaaatgcat cagatcctct ctgaatatga
    11881 catcaacctg gtgatcatgg atatcaatct gccgggtaag aacggtcttc tgttagcgcg
    11941 tgaactgcgc gagcaggcga atgttgcgtt gatgttcctg actggccgtg acaacgaagt
    12001 cgataaaatt ctcggcctcg aaatcggtgc agatgactac atcaccaaac cgttcaaccc
    12061 gcgtgaactg acgattcgtg cacgcaacct gctgtcccgt accatgaatc tgggtactgt
    12121 cagcgaagaa cgtcgtagcg ttgaaagcta caagttcaat ggttgggaac tggacatcaa
    12181 cagccgttcg ttgatcggcc ctgatggcga gcagtacaag ctgccgcgca gcgagttccg
    12241 cgccatgctt cacttctgtg aaaacccagg caaaattcag tctcgtgctg aactgctgaa
    12301 gaaaatgacc ggccgtgagc tgaaaccgca cgaccgtact gtagacgtga cgatccgccg
    12361 tattcgtaaa catttcgaat ctacgccgga tacgccggaa atcatcgcca ccattcacgg
    12421 tgaaggttat cgcttctgcg gtgatctgga agattaatcg gctttaccac cgtcaaaaaa
    12481 acggcgcttt ttagcgccgt ttttattttt caaccttatt tccagatacg taattcatcg
    12541 tccgttgtaa cttctttact ggctttcatt ttcggcagtg aaaacgcata ccagtctata
    12601 ttacgagtca caaacatcat gccggccagc gccaccacca gcacactggt tcccaacaac
    12661 agcgcgctat cggcagagtt gagcagtccc cacatcactc catccagcaa caacagcgcg
    12721 agggtaaaca acatgctatt gcgccagcct ttcaataccg cctgcaaata aataccgttc
    12781 attaacgccc caatcagact ggcgattatc catgccacgg taaaaccgat atgttcagaa
    12841 agcgccagca agagcaaata aaacatcacc aatgaaagcc ccaccagcaa atattgcatt
    12901 gggtgtaaac gttgcgcggt gagcgtttca aaaacaaaga acgccataaa agtcagtgca
    12961 atcagcagaa tggcgtactt agtcgcccgg tccgttaatt ggtattgatc ggctggcgtc
    13021 gtcactgcga cgctaaacgc ggggaagttt tcccagccgg tatcattgcc tgaagcaaaa
    13081 cgctcaccga gattattagc aaaccagctg ctttgccatt gcgcctgaaa acctgactcg
    13141 ctagcttccc gtttggctgg tagaaaatca cctaaaaaac tgggatgcgg ccagttgctg
    13201 gttaaggtca tttcgctatt acgcccggca ggcaccacag aaagatcgcc ggtaccgctt
    13261 aaattcagtg ccatattcag ctgcaggttc tgcttacgcc agtccccttc gggtaaaggg
    13321 atatgcacgc cctgcccgcc ttgctctaac ccggtaccgg gttcaatggt cagtgccgtt
    13381 ccgttaactt caggcgcttt caccacacca ataccacgcg catccccgac gctaatcaca
    13441 ataaagggct tgcctaagat gatatttggc gcgttgagct cgctaagacg cgaaacatcg
    13501 aaatcggctt ttagcgttaa atcactgtgc cagacctgac cggtataaat ccctatcttg
    13561 cgttcttcca cgttctgatt gccatcaacc atcaatgact caggtaacca aaaatggata
    13621 aaacttcgtt tccgctccac ggttttatcc tcttcctgca ccgtataaag ctcggtcaca
    13681 ggaatagcga taagcggccc aacgagtttt tgcggcccgc tggtactttg gcgaatcgca
    13741 tcttccacat cgctacggta atcggcacgt tcgacaatca cttgccgaat cagcattatc
    13801 ggaatcaaca acagcaatac tgcaccaaac aaggtggtca ttttccagaa caggggggat
    13861 ttcaacatag tcttctcctt tgcaatggca gcaggatacg aaggctatgt gggaagaatt
    13921 tgaagctatg tgaagtgacg gtgaagtcgt agcgaggcca gcacgccacc ttcctgcacg
    13981 ttgcgcagcg tgacttcgcc gttaaacaaa cgggcgacct cactgacgaa cgccaacccc
    14041 agaccgctgc ttttttgccc atttgcacgc ggcaaagagt aaaagcgttc aaaaatacgt
    14101 gacagcgcgt agtcaggaat accactaccg gtatccagca ccttaagcgt gacgtgttcc
    14161 tgatccactt cggcgcttag cgttatgcaa ccgctctcgg gagtaaaatc gatggcgtta
    14221 tccagtaaat tccccagcgc ctgatccagt aacgccggtt cagcagcaac attaacctcg
    14281 gtgggcataa catgcaaagt gatgtttttt tctgccaact gcacggtgcg cgcttcgctg
    14341 acgcggcgaa ataatgccgc cacatcaaca gcagtcagaa cgacttcctg acgattctcc
    14401 agtcttgcct ggcgtagtaa cgtttccacc agcgcctgca ttcgcgcatt ttgtgtcaga
    14461 atgttgtcag taaaacgagc cactacttca ggcggcggac cttcgcgtaa aatttccgcc
    14521 gcgccacgaa tcgccgccag tgggcttttt agctcatggg ttaacgcata aacatactgc
    14581 tcaatatagt ttttcccttc cagcttcacg cgcatacttt ccagcgcctg cgcgagttta
    14641 cgcagctcgc tactaccgag atcgggaaga gaaacgggct tattgtcagt gacggaatca
    14701 gcatagcgag tgagccgggc aatggagcgg ttgatccacc aaaccatgcc tgcgccaatc
    14761 accagtgcaa tccccaacaa aatggcgctg gcccataaaa ttcgccgctc gctgcgttta
    14821 atgaccggag ccatcgccgc gttcggtttg cctacgctca aaacgccaat aagccgcgag
    14881 ccgcccataa tcggcgcggc aacatacatc accgaacttt cgggatcggc aggattttgc
    14941 aacgtgctgc gcgcaccata ctgaccacgc aacgttagcc agacgtcatt ccagcgcgaa
    15001 taatcctgtc caacggcttt atttgccgaa tcgaacaata ctttgccatg cgcatcggtc
    15061 atatagacat gatattcatt gcgcactttg ttaatgccac caatattggc gcgaaacggg
    15121 cgatgttgta gctgattaaa cgcctgcgcc agttgcccat gcgttgggtc cccagagagc
    15181 aaatcgggac gcgccagctc cgcaagtaac gttgccgtgt cgatcaacgt cccctccgtt
    15241 gctcttcgca cgcccggttt aacttctttg acaaaaatag ccagtacgaa ccaggccgcc
    15301 accgccacca gtaaaaaata gcccagcagc aaccgcatgc cgatacgcat tacaagcccc
    15361 tcaggctata tcccatgccg cgatgagtat taatcggtga aagatcgggg ttgatggcgc
    15421 gcagcttggc acgcagcgtt ttaatgtgga tatcgacggt gcgatcgtag gtgtcctgcg
    15481 catcttccca cacgctatcc attagttgct ggcgggacca gacgcggcct ggcgacttga
    15541 gtaacgtctt cagcaataaa aactcatacc gcgtcagcgt taatggcgtg tcaaaccagc
    15601 tgatctgcgc cgcgggttca ttcaattcaa aatgaccaat acggatgacg ggagacggcg
    15661 tcgagaactt cttcacccga cgcagtaagg tgcgcaccct ggcgcacact tcgcggggtg
    15721 aaaacggttt agccacatag tcgtcagcgc caatttccag cccaagcagg cgatcgacct
    15781 cttcacttcg ggccgtcagg aacagtacag gtaacgccgg atggagcgcc agtaactggc
    15841 ggcacaattc aaagccactg atatcaggca aaccaacgtc gagaatcatg acgtcgggaa
    15901 cctgctggcg agctttatcc agcaccggca aacctcgctc aaagacctcg acggcaaaac
    15961 cttcctgctg caacatgtag accagcgtgt cggctatccc ttgctcatct tccactaacc
    16021 agaccgtttc ccgttgcata aatcgcctct gttattgccg ccacggcatg acaggaaccg
    16081 cgctaatcgc gtttttcggc gaaccttcta caactttgtc ggagtaagcc agataagcga
    16141 gcgcgttgcg tttggcgtca taaaagcgca cgacctgtaa cgacttaaag accagggacg
    16201 tgcgtttttt gaatactacc tcgccctgag ctttgccgtt tttaatacga tccgacagtt
    16261 caatcggccc gacttgctga caagaaatgg ccgcatcgga ggtatcttct gccaggccca
    16321 atcccccttt aataccaccg gttttcgccc ggctcacata acaggtgaca tttttcacat
    16381 cgggatcgtc aaaggcttcc acaacaattt tgtgatccgg gccgatcatt ttaaatacgg
    16441 tgtcgaccga accaatctct tctgcatgag ccaatggccc cagcattatc aggcttaaag
    16501 acaggatcaa atgcttgtat ttcatattgt taccattttt atctattaca cgagcaatta
    16561 gttcgtcacg gtaaataaca tattgattaa gaacaaaatc tcaattcttt tatttccgtc
    16621 aagccctaaa acatactcta ctaagaaaaa aacactgaat gctaaaacag caaaaaatgc
    16681 tattatccaa ttacctgatg tcaggtgctc gttgttgaaa ggatgaggat attttatgga
    16741 tcaggccggc attattcgcg accttttaat ctggctggaa ggtcatctgg atcagcccct
    16801 gtcgctcgac aatgtagcgg cgaaagcagg ttattccaag tggcacttac agagaatgtt
    16861 taaagatgtc actggccatg ctattggcgc gtatattcgt gctcgtcgtt tgtcgaaatc
    16921 ggcggtcgca ctacgcctga ctgcgcgtcc gattctggac atcgcgctgc aataccgctt
    16981 cgactctcaa cagacattta cccgcgcatt caagaagcag tttgcccaga ctcctgcact
    17041 ttaccgccgt tctcctgaat ggagcgcctt tggtattcgc ccgccgctac gtctgggtga
    17101 attcacaatg ccagagcaca aatttgtcac cctggaagat acgccgctga ttggtgttac
    17161 ccagagctac tcctgttcgc tggagcaaat ctctgatttc cgccatgaaa tgcgttatca
    17221 gttctggcac gattttctcg gcaacgcgcc gaccattccg ccggtgctct acggcctgaa
    17281 tgaaacgcgt ccgagtcagg ataaagacga cgaacaagag gtattctata ccaccgcgtt
    17341 agcccaggat caggcagatg gctatgtact gacggggcat ccggtgatgc tgcagggcgg
    17401 cgaatatgtg atgtttacct atgaaggtct gggaaccggc gtgcaggagt ttatcctgac
    17461 ggtatacgga acgtgcatgc caatgctcaa tctgacgcgc cgtaaaggtc aggatattga
    17521 gcgatactac ccggcagaag acgccaaagc gggagatcgc ccaattaatc tgcgctgtga
    17581 actgctgatt cctatccgcc gttaacgctg cagctcatct aatgcagggg cgtccagatg
    17641 cgagatgtcc cctgcggttt caacgaccca gccagacgcc agccacaggc tttcctgata
    17701 atccacgcgc gaaatagaac agttacgcag acgtaagcgg cgctctgccc atgctggtaa
    17761 tccgagaatc gtactcacca gacatcccag tgcaatacca tggctcacca gcagcggtcg
    17821 gcttccctgc ggtaagtccc ggcaagattc cagtgccgca ttgacgcgat cgctgagctc
    17881 ctgcattgac tcgccttcag gaatacgccc gtcaacggtg ccattgacca gctgccgacg
    17941 ccagttctct tcttcttcgg tcagagaatc gatatgtctt ttttccagca cacccatgtt
    18001 taattcacgc aggcgagaat caaagatgat gtcacagccg caggcctggg cgatgatttc
    18061 cgccgtacgc cgggtgcgtc ctaaatcgct actgataata tgcgtaatgc caagctcttt
    18121 ggcacgggtt gccacctgca tcgcctgttg ctcacctttg gcggtcagcg ggctgtcaga
    18181 ctggccctga atacgtcgct cggcgttcca ctgcgtttca ccgtggcgga ctaggtatac
    18241 ctgtaacatg ctttttttcc gtatactgct gtaacgttaa ttttctgagt aatgctgatt
    18301 atgcaccaag ttgtctgtgc gaccaccaat cccgctaaaa ttcaggccat tctgcaggca
    18361 tttcacgaga tcttcggcga aggatcctgc catattgcat ccgttgccgt cgagagcggt
    18421 gtaccggaac aaccctttgg cagtgaggaa acgcgcgctg gcgcacgaaa tcgggtagca
    18481 aatgcccgcc gtttacttcc agaggctgat ttttgggtgg cgattgaagc tggcattgat
    18541 ggcgacagca ctttcagttg ggtggtgatt gaaaacgcca gccagcgcgg cgaggcgcgt
    18601 tcggcaactt tgccgttacc tgcggtgatt ctggaaaaag ttcgtgaagg cgaggcgctt
    18661 ggtcccgtga gtcgcgttat accggtattg atgagattgg tcgtaaggaa ggggcgatcg
    18721 gcgtgtttac cgccgggaaa ctcactcgcg ccagcgtcta tcaccaggcg gtgatcctgg
    18781 cgctgagtcc gtttcacaat gccgtgtatc aggcattgta ggacggataa ggcgttcacg
    18841 ccgcatccga cattcggtgc acgatgcctg atgcgacgct gccgcgtctt atcaggccta
    18901 caaaatcaat cgcttttcag caacacctct tccagccact gacgcagctc cacgggcgcg
    18961 gctttcaggc tgttagatcc acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt
    19021 aactcacgct gactcatttc gccacgcaac agctcttcga caatacgcac gcgagtcccc
    19081 aacgcttcgc gctcatctgg cgtcagcatc aggtttaaca acggtaaatg gagatcgttt
    19141 tggtaggcat tcttaagcag gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc
    19201 atcgctgctg aatagggtga ttgttgggcc ataatatgtc gccattgtta gcgggggaag
    19261 caaaatgact cccccggttg tactcgctaa agagtacgat agcatatcat aaacgtgcgg
    19321 atcagtaacg acgtccccat tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat
    19381 agcggtagta agcatcataa gccagcacgt tcttcacata accgcgcgtc tcggagaacg
    19441 gaatactctc gacaaatgcc acagcgtcga tacgcccggc gctgtggcca agccaggttc
    19501 gcacccgtcc aggtccggcg ttataagctg ctgaggagaa aatacggtta ttgccaaact
    19561 gctgataaac atattgcagg taactggtgc caatgttgat gttcgtttcc ggatccagca
    19621 attgcccagg gctgctataa ccaggaatag agaacatctt caccgtatgg gtcgctgtac
    19681 caggcataat ctgcatcagg ccgctggccc ctaccggtga tttcactttc ggattccagg
    19741 cgctctcctg acgggcaatc gccatcgcat agctttgcgg gatctctttc ccactggtgt
    19801 aacgtttgaa aagatcgttg taagccagcg ggaatcgctc ttccagatga tcccaaagct
    19861 tcccggcgat cgttgcctga acgctaagat cccaccattg gttattgaaa gcataccgag
    19921 ccagttgagc ctgctctgtt tttgacttgc tcttcaccag attggcccac tcgctacgcg
    19981 cggtattatc gagattccag tacatcaact cgcgcacgcg cgccatctcc ggcccctgag
    20041 tcagggtgct gtcaacattc tgcggcgctt tatcaatctt cagctcatac tcttcaccga
    20101 tgcgttgtgc tgcaaccatc gggtagaaac cacgctgttg tatgagttga tgcaaaatct
    20161 ctttcgcttc agcttcacgt ccgcgttcca gcaataaatc cgcctgccag taacgccatt
    20221 catctttctc ttttgcttcc atcggcagac gcgccagcca ggtattcagg ccgcgacgat
    20281 cgccggtgcc aagcgccatt cgcacacggc gttcaatcaa cgacgtggat tgcgagcgca
    20341 taatggcgtc atcgcgccat ttcgcctgct catcggtgac atcattgccc atcaaacgcc
    20401 aggcgacgat atcgcgcagc tcctgaatct gatcttcatt aagctgctgc gcctgggcaa
    20461 gcgacgggat catcagccgt gcattttcgg catcctgccg cgccacactg gcaaacgcca
    20521 ccgccgccat ttgacgggta aaatcggtcg cgccggttgt acgcgcgaag gtcagtaccg
    20581 tattagggtt gttcgccagt gaaatgattg ccgaggcgat agtctggtaa tcggcaggca
    20641 tctgccctgc cagcactgtt accaggcctg tgttacccgc tttcatcgcc agacggatac
    20701 gctctaaata cgccagcgga tcttgtttac ctgacgcacg ccagacgcta aataacttgt
    20761 cacaggcgtt aggctggctc ttgccggtta gccacagctc tttcgcccct tgccaggctt
    20821 cttcactctg cccggtgttc catttcgcat agtagtaatt acattgcgct tcggtagttc
    20881 cgggcttttc cgggctaaag gctaacaagc cacgccagtc ttcacgccgc gccagttcat
    20941 tgacgaaacg agattgcagc gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt
    21001 tagtgaccgt caccgccggt tgattcatca ggtcatcggt gatctggcgg tattccaggt
    21061 agggataaag cggataatcc ttcagtccag gcatcatttg ttcgaccaca tccatctgtc
    21121 gattatccca ggcctgcttg atttgcgcgt aacggctacg ctgctcatcc agtgagtcgg
    21181 ctcgcgccac gctgctgacc gtcagcagac agacaccggc agccaacagc cgccaggtaa
    21241 cttgtttggc tttttccaca agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat
    21301 gccgcgtaat agatttatgc taaccagtca ttgccgttta cgccacgtta cggacacttt
    21361 tttactttta ctgcgaaggt gatcgaccaa ctgggattag gcggggaaac aggctacact
    21421 tcgcctttga aaactcatca tcacgataaa catagaggcg aagtccaacg tggctcaatt
    21481 cgtttatacc atgcatcgtg tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa
    21541 catctctctg agtttcttcc ctggggcaaa aattggtgtc ctgggtctga acggcgcggg
    21601 taagtccacc ctgctgcgca ttatggcggg cattgataaa gacatcgaag gtgaagcgcg
    21661 tccgcagcca gacatcaaga ttggttatct gccgcaggaa ccgcagctga acccggaaca
    21721 caccgtgcgt gagtccattg aagaagcggt ttcagaagtg gttaacgccc tgaaacgcct
    21781 ggatgaagtg tatgcgctgt acgccgatcc ggatgccgat tttgacaagc tggccgctga
    21841 acaaggccgt ctggaagaga tcattcaggc tcacgacggt cataacctga acgtacagct
    21901 ggagcgtgcg gcggatgcgc tgcgtctgcc agattgggac gcgaaaatcg ctaacctctc
    21961 cggtggtgag cgtcgtcgcg tggcgttgtg ccgcctgctg ctggaaaaac cagacatgct
    22021 gctgctcgac gaaccgacca accacctgga tgccgaatcc gtggcgtggc tggaacgctt
    22081 cctgcacgac ttcgaaggca ccgtggtggc gattactcac gaccgttact tcctcgataa
    22141 cgttgcaggc tggatcctcg aactggaccg cggtgaaggt attccgtggg aaggtaacta
    22201 ctcctcctgg ctggagcaga aagatcagcg tctggcgcag gaagcttcac aagaagcggc
    22261 gcgtcgtaag tcgatcgaga aagagctgga gtgggtacgt cagggaacta aaggccgcca
    22321 gtcgaaaggt aaagcacgtc tggcacgctt tgaagagctg aacagcaccg aataccagaa
    22381 acgtaacgaa accaacgaac tgtttattcc acctggaccg cgtctgggcg ataaagtgct
    22441 ggaagtcagc aacctgcgta aatcctacgg cgatcgcctg ctgattgatg acctgagctt
    22501 ctcgatcccg aaaggggcga tcgtcgggat catcggtccg aacggcgcgg gtaaatcgac
    22561 cctgttccgt atgatctccg gtcaggaaca gccggacagc ggcaccatca ctttgggtga
    22621 aacggtgaaa ctggcatcgg ttgatcagtt ccgtgactca atggataaca gcaaaaccgt
    22681 ttgggaagaa gtttccggcg ggctggatat tatgaagatc ggcaacaccg agatgccaag
    22741 ccgcgcctac gttggccgct ttaactttaa aggggttgat cagggtaaac gtgttggcga
    22801 actttccggt ggtgagcgcg gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa
    22861 catgctgctg ctcgacgaac caaccaacga cctggatatc gaaaccctgc gcgcgctgga
    22921 aaacgccctg ctggagttcc cgggctgtgc gatggttatc tcgcacgacc gttggttcct
    22981 cgaccgtatc gccacgcaca tcctggacta ccaggatgaa ggtaaagttg agttcttcga
    23041 aggtaacttt actgagtacg aagagtacaa aaaacgcacg ctgggcgcag acgcgctgga
    23101 gccgaagcgt atcaagtaca agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg
    23161 cgaacgcctt atccggccta caaaaccagc aatttcaata cgttgtaaaa ctgtaggcct
    23221 gataagacgc ggtaagcgtc gcatcaggca ttgagcaccg aatgccggat gcggcgtgaa
    23281 caccttttcc ggtctacaac agccttagca tcctcaattt cgtggcgggt cacccgtgca
    23341 ggttttttca aaccttgttt aatctcgaca gcctcttcca gagaggtttt caactcatca
    23401 aaaaaactca ttttatacct cgcctttgag cagtttcgtc acggttatcc ttgctccccc
    23461 atcatctccc gcaccagctc cacgcagcgc aggaaacggc tgtcgtaatc gtcctcttca
    23521 acccgcacga attcgatatt gttctcttcc agcatctcca ccagcaagtt ctggaactct
    23581 ttgcgatcca ccgaactgcc gaggctgcgt aaaccatccg ccacccacgg cgtgttgttc
    23641 tccagcagga tcaccagatc gaaacggtat tcatcaatca gcgcctgcac gaacggatgc
    23701 tcacgccctt cgtacttttt gcagaacgcc tgggtagtga caaaatcggt gtcaataaac
    23761 gccactttat tggcgtattt cactgcaaaa tcaatgtatt gtgcgtggcc caacgcgatt
    23821 ttatcgtagt cggaatactg caatgcgatc tcatcaccgc cgaggtgtga aaagacataa
    23881 tcgcggccat attcccacgc actggtggtg ttgaagatat tggcaagttt gtttaccagg
    23941 gtggatttac cgctcgactc gccgccaagg atcgccacgg tacgcacaaa aaacggcttc
    24001 acttcggtag gaatatattc ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg
    24061 atactcataa aggtacgttt cggatcgacc agcaccgtct cgatccccag atgttccata
    24121 tactgtggcg catcggcttc ttccgaggtg tagatcaggt ccggctggat ccccttttca
    24181 gccataaact ttttgatgcc gttgctccac acatcccagc catgcggata cggctccatg
    24241 ccctcttcgt tgaaagcatg aatacggata tttttctgat atttaaaggt ttgcaatagc
    24301 cagcgcagac gatccggcac cgtgggctgc tgcgacatgg cgctgtcttc aaacaacgcg
    24361 cggtcgcggg tatcgtcaaa acccataatg atatgcagct cgtcaacctg gctacaggcg
    24421 cgctggataa ggtagatatg tccggtatgc agcgggtaga acttaccgaa tacgacacca
    24481 atggttttct tctgccgggg aaattcaagc cccaaaaaac ggtgcaatgc ctccagcttt
    24541 tgcgcgctgg ggcttttaat tttggcattc agtaactggc ttaaataccc tttggtcata
    24601 ccgctggcat cagctacctg ctgtagcgtg cagccctgtt gcttgatggc agttttcagg
    24661 taatcaaatg acgacatatc tccctccgta tctctcatta taagtcgtcg aacacgctaa
    24721 gcgcgtcgga gagtttttta acgccaaaaa tctgcatccc ttccggcgct tttttcggta
    24781 cgttagccgc cggaacaatc gcccggcgaa aaccgtgttt cgccgcttca gaaatccgct
    24841 cctggccact gggcaccggg cggatctccc ctgccagccc gacttcacca aacaccacca
    24901 gatcctgtgg cagcggtctg tcacgcaggc tggaaaccat cgccagcagt aacgctaagt
    24961 cggcgctggt ttcggttacc ttcacgccgc cgaccacgtt cacaaacaca tcctgatcgg
    25021 ccatttgcag accaccgtga cggtgcaaga cagccagcag gattgccaga cggttttgtt
    25081 ccagccccac tgccacgcgg cgcggattcg ccattatcga gtgatcgacc agcgcctgaa
    25141 tctccaccag caacggacgc gttccttccc acaccaccat cacggagctg ccggaggtca
    25201 cttcatctcc gcgacttaag aaaattgccg aagggttgct gacttcacgc agcccctgtt
    25261 cggtcatcgc gaagacgccc agctcattca ccgcgccgaa gcggttttta tggctgcgta
    25321 aggtgcgaaa acgggagtcg gcatcgccat ccagaagcac cgaacagtcg atacagtgtt
    25381 ccagcacttt cgggccagcc agcgaaccat ctttggttac gtgtccaacc atgacaatcg
    25441 ccacaccgcg cgttttggcg aaacgcgtca gataagccgc cgtttcacgc acctgcgcca
    25501 cgctaccagg cgatgattgt acatccgcca tatgcatcac ctggatcgag tcaattacca
    25561 tcagcttcgg ttgctcttct tcggcaatca ggcagatctg ctcgatgctg gtttccgaca
    25621 acatattgag attgtcagtc ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca
    25681 gcgactcttc gccggtgaca tacagcgttt tcatctgctg ggccagtttg cacagcgttt
    25741 gcagcaacag cgtggatttc cccgcaccag ggttaccgcc aatcagaatg gcacttcctg
    25801 gcaccacgcc gcctcctagt acgcggtcga actctttaaa tccggtggaa aaacgcggca
    25861 gctcttcaag gctgatatcg gagagtttct ggacttttgc caccccggcg ctaccggcat
    25921 agccgctgag acgctcgtta cgcgccaccg ttggcgacgc agcaagacgc acctcggtga
    25981 tggtgttcca ggcatgacag gcactgcact gcccctgcca gcgcggataa tcggccccgc
    26041 attcattaca aacaaaggcg cgttttggag cttttgccac ggtgtacctc gttaatgctg
    26101 tgccgcccgc aggatgacgg gcaggcaatt acttctgatt caggctgcca gagaggatgc
    26161 agaatacccc catcaggtca gcgtgacgga tggtgacttc cgccttttca ttcacttttg
    26221 gcttggcatg gtaggcaatc cccagccctg ctgctttgat catcggcagg tcattagctc
    26281 catcgccaat cgccacggtc tgcgccagcg ggatttcata ctcctgcgcg aggcgagtca
    26341 gagttttcgc tttgtactgc gcgtctacga tgtcgccgat cacattgccg gtaaatttac
    26401 cgtccatgat ctccagttca ttggctacca cggctgtcag gcgcagcttg tcgcgcaggt
    26461 attcagcaaa gaaagtaaag ccgccggagg caatcgccac tttccagccc agcgtttcca
    26521 gcttgagcac cagctgcgtt aagcctggca tcagcggcag attttcacgc acctgttgca
    26581 gaatattggc gtcagcgcct ttcagcgtcg ccacgcggct gcgcaggctg gcggtaaaat
    26641 cgagttcgcc gcgcatcgcc cgttcggtta cctccgccac catctcgccc gttccggcca
    26701 gcttggcaat ttcatcaata cattcaatct ggatggcggt ggaatccatg tccatcacca
    26761 gcaaacccgg cgtgcgcagg tgcgggattt tccccagcgg ggcgacatcc agctgcgctt
    26821 cgtgggccag gcgtgtagcc cgtgcggtga gtgaacctgc cagacgaatc acctgataat
    26881 cttccacgca ccaggcggca acaatcacca tcgccgcacc cagtttgctc tggtattggg
    26941 tcagacgctg tttatccagc ccacgaccat acagcagcca gccgctacga cctgcgtggt
    27001 aatccagtgg catcacttca tcaccactta atgaaagagg cagacccggc cataaagaga
    27061 catcttcagg caggtcgcac caggtaatgt taggcattaa ggctcctgta aaatcgttcg
    27121 aagcagggaa aataacgcat gaggctacct tgtatccatt gcttctggca acattaagtc
    27181 tcaaattttc aaagggtgga agatggctcg cacaaaactg aaattccggc tacatcgggc
    27241 agtgattgtc ctgttctgtc ttgccttgtt agtggcactg atgcaggggg cgtcatggtt
    27301 tagtcaaaac caccagcgac agcgtaatcc acagctggaa gaactggccc gcaccctggc
    27361 gcgtcaggtg acgctgaacg ttgcaccgct gatgcgtacc gactcaccgg atgaaaaacg
    27421 cattcaggcg atcctcgatc agttaacaga tgaaagccgt atcctcgacg cgggtgtgta
    27481 tgacgaacaa ggcgatctta tcgcacgttc tggcgaaagc gtcgaagtgc gcgatcggct
    27541 ggcgctcgac ggtaaaaaag caggcggcta ttttaaccag cagattgtcg agccaattgc
    27601 gggtaaaaac ggaccgctcg gctatctgcg cctgacactc gacacccata cgctcgccac
    27661 cgaagcccaa caggtggata acaccactaa cattttacgc ctgatgttgc tgctctcact
    27721 ggcaatcggc gtagtgctga cccgcacgct gctacagggt aaacgcaccc gctggcagca
    27781 atcgcccttc ctgttaaccg ccagcaaacc ggtgccggaa gaggaagaaa gcgagaaaaa
    27841 agagtgaccc attactacaa gaaaggaaat cgttatgtcc acattacgcc tgctcatctc
    27901 tgactcttac gacccgtggt ttaacctggc ggtggaagag tgtatttttc gccaaatgcc
    27961 cgccacgcag cgcgttctgt ttctctggcg caatgccgac acggtagtaa ttggtcgcgc
    28021 gcagaacccg tggaaagagt gtaatacccg gcggatggaa gaagataacg tccgcctggc
    28081 gcggcgcagt ggcggtggcg gcgcggtgtt ccacgatctc ggcaatacct gctttacctt
    28141 tatggctggc aagccggagt acgataaaac tatctccacg tcgattgtgc tcaatgcgct
    28201 gaacgcgctc ggcgtcagcg ccgaagcgtc cggacgtaac gatctggtgg tgaaaaccgt
    28261 cgaaggcgac cgcaaagtct caggctcggc ctatcgcgaa accaaagatc gcggcttcca
    28321 ccacggcacc ttgctactca atgccgacct cagccgcctg gcaaactatc tcaatccgga
    28381 taaaaagaaa ctggcggcga aaggcattac ttcagtgcgt tcccgcgtga ccaacctcac
    28441 cgagctgctg ccggggctca ccatgagcag gtttgcgagg ccataaccga ggcctttttc
    28501 gcccattatg gcgagcacgt ggaagcggaa atcatctccc cggacaaaac gccagacttg
    28561 ccaaacttcg ccgaaacctt tgcccgtcag agtagctgag agtggaactt cggtcaggct
    28621 ccggcattct cgcatctgct ggatgaacgc tttagctggg gcggcgtgga actgcatttc
    28681 gacgttgaaa aaggccatat cacccgcgcc caggtattta ccgacagcct caaccccgcg
    28741 ccgctggaag ccctcgccgg gcgactgcaa ggctgcctgt accgcgcgga tatgctgcaa
    28801 caagagtgcg aagcgctgtt ggttgacttc ccggaccagg aaaaagagct acgggagttg
    28861 tcgacgtgga tagcgggggc ggtaaggtaa ctcgctgatt aaaatatcca aggaaggaaa
    28921 tatgatcgat ctggaaaacc aggaacggga gattatcaat ttaatgttca gccagcgtat
    28981 ttcctggcta gccgccgtgc gtatcaggca taaattatcg cttgctgaag taagtaaaat
    29041 gttaggtatc tcgattaatt ctctaaaaca gatcgaaaag acggaacggc ttagctcaaa
    29101 catcaaaagc aaaatggcag aaatttatgg ttgtccacca gagttgctga tatgcccttc
    29161 ctggatgacg gctgaacata aatgaataga ttcgtgaata gatacttgaa tgttttttat
    29221 tttctatcca tatgaaaaat aatagtaaat ctataaatca gttgctattg atgaatagat
    29281 actaaaaagc cggagcagtc ccccactccg gcttaacacg acaatcgcct ttcagcgaaa
    29341 cacaattact ctttatcgcc cagcagaacg gattccagtg cgattttgat catgtcgttg
    29401 aaggtggtct gacgctcagc ggcagtggtc tgctcgtgag tgcggatgtg gtcagacacg
    29461 gtgcagatgg tcagggcttt cgcgccaaac tctgcagcca cgccgtagat accagccgct
    29521 tccatttcca cgccgaggat gccgtatttt tccatcacgt cgaacatttc gccgtccgga
    29581 gagtagaaca ggtcagcgga gaacaggtta cccacgcgag catcaacgcc cagtgcttta
    29641 gctgcatcta ctgcgttacg caccatgtca aagtcagcga tagcggcaaa gtcatggtct
    29701 ttaaaacgga tgcggttaac tttggaatcg gtgcaggccc ccataccgat aacgacgtcg
    29761 cgcagtttta cgtgcggcag aactgcgcca caggaaccaa cgcggataat tttcttcacg
    29821 ccgaaatcgg tgatcagttc tttggtgtag atggagcagg acgggatacc cataccgtga
    29881 cccattacgg aaattttgcg gcctttgtaa gtaccagtga agcccagcat accgcgaacg
    29941 ttgttcactt cacgggcatc ttcaaggaaa gtttcagcaa tatacttcgc acgcagcggg
    30001 tcgcctggca tcaaaactac gtcagcgaaa tcgcccattt ctgcattaat gtgtggggta
    30061 gccattgttt tatccttttt gtgacataac aaaggcggag tcaacgctcc gcccaaatcc
    30121 atcagaacat ggctttgcca tattccatat cagaagtacc aaaatatttt gccagagtct
    30181 ggccgatatc cgcgaaggtt tcacggtgac ccagtgagcc cggttttact ttcgggccat
    30241 ataccagtac cggaatgtgt tcacgcgtgt ggtcagtacc ggtccaggtc ggatcgcaac
    30301 cgtggtcagc ggtgaggatc aggatgtcgt catcgcgcag cagagacatc agctccggca
    30361 gacggcggtc gaacagttcc agacccgcag cataaccggc gacgtcgcgg cggtggcccc
    30421 aggaagagtc gaagtcaacg aagttggtga agacgatggt gttatcaccc gcttctttca
    30481 tctctttgat ggtggcgtca aacagcgcgt ccaagccagt cgctttcact tttttggtga
    30541 taccgcagtt ggcgtagatg tctgcaattt taccgacaga aaccacctgg ccgtgttttt
    30601 catcaaccag tttctgcagc acggtcggtg ctggcggctc aacagccaag tcgtgacggt
    30661 taccggtacg ctggaagtta ccagctttgt caccgataaa cggacgagcg ataacacgac
    30721 cgatattgta gccgccgttg gtcagctctt cacgggcgat ttcgcacagt tcgtagagtt
    30781 tatccagacc gaaagtttct tcatggcagg caatctggaa cacggagtca gcggaggtat
    30841 agaaaatcgg cttgccggtt ttcatgtgct cttcgcccag ttgatccaga atgaccgtac
    30901 cggaagagtg gcagttaccg aggtaacccg gcagattagc gcgttcgacc agtttatcca
    30961 gcagctcttg cgggaagctg ttttcgtgat cggagaaata tccccactca aacagaaccg
    31021 ggacaccggc gatttcccag tgaccagacg gggtatcttt accggatgac atttcgtgcg
    31081 cccatgcgta cgcgccgata acttcagcgt tgccgtccat tcccgccgga atgaaaccgg
    31141 tagaaccttc gtgtgctttc gccagcccca gacgggtcag atttggcaga ttgagcggcc
    31201 ctttacgacc attatcagct tcgcctttgg cacaagcttc tgcgatatga cccagggtgt
    31261 cagccccgac gtcaccaaag cgttctgcat cttctgtagc gccgatgccg aatgagtcca
    31321 gcaccataat aaatgcacgt ttcatatgtt ctccgtactc agtgcttcaa atttaaatga
    31381 tcagatcagt ataccgttat tcgctgatac ggcgatagac agttggtgtg ctttccggtg
    31441 cgttatcggc aagtttaatt gccgctttca ccgctttcgc cgcttcctgc cagctgtttt
    31501 cgtctttcgc gaggataacc gccagcggac gctgaccgtc tacctggtcg cccagacgcg
    31561 ccatatcagt aaagccgacg ctgtaatcga tggtgtcaga tgcctgacga cgaccgccgc
    31621 ccattgcaac cactgccatc cccagcgcgc gggtatccat ttcactgaca aaaccttcgg
    31681 tatcagcata gactgctttc gtcagcatcg ctgtcggcag atacttcgcg tagttctcaa
    31741 cgaagtcggt cgggcctttt tgtgccgcta ccatacgacc aaagacttct gccgctttac
    31801 cgttgtccag caccgcctgc aatttcgcgc gcgcttcggc gtcatctttc gccagtttgc
    31861 cggagatcag catctccacg cacagcgcca tcgtgacatc aaacagacgc gggttacgat
    31921 actcacccgt caggaactgc accgcttcac gaacttcaac cgcgttacct gcactggagg
    31981 ccagtacctg attcatgtcg gtgagcagcg cggtggtgcg cacgccagcg ccgttagcca
    32041 cgccaacaat cgcttcggca agggcttcag agagttcgta ggtcggcata aacgcgccgc
    32101 tacccacttt cacgtccatc accagcgcgt ccagaccttc cgccagtttc ttcgccagga
    32161 tcgaggcagt gatcagcggg atggagtcca cggttgcggt aatatcacgg gtcgcgtaga
    32221 aacgtttatc agccggagcc agtgaactgg tctgaccgat aatcgccacg ccgacgtctt
    32281 taataatttc gcggaaacgg ttgtcatccg ggaaaatgtc gaagccaggg atggattcca
    32341 gtttgtcgag cgtaccgcca gtatgaccga ggccgcgacc ggagatcatc ggaatatagc
    32401 cgccgcaggc tgcgaccatc ggccccaaca tcagcgaagt gacatcgccg acgccgccgg
    32461 tcgagtgttt atcaacaatc gggccattca gatgcaggct tttccagtcg agaactgttc
    32521 ctgaatctcg catcgccatg gtcagcgaga cacgctcagg cattgtcata tcgtggaaga
    32581 aaatggtcat cgcgagggcg gcaatctgcc cttcggaaat agtgttgtcg cgaataccgt
    32641 tgataaagaa acgaatttct tcatcgctca gcgcatgacc atcacgtttt ttacgaataa
    32701 tttcttgtgc gagaaacaag gtaacctccc gaggaaaaga gtatgaggcg gcttttgccg
    32761 gatgcgtcta cgccttatcc ggcctacggg tagatacgtc tcgtaggcct gataagcgca
    32821 gcgcatcagg cgtaaagcat cttacttagt agctgctggc gctcttaccg tcgccgtggc
    32881 ccagcgcttt cagcaggctt gccagcaggc tggaagcacc aaagcggtag tgacgcgcat
    32941 cagcccagtc agcaccgaac agttcatctg cgatggcgag atatttctgc gcatcttccg
    33001 cagtacgcac gccgcccgcc ggtttgaaac caacggtttt ttctacgccc atatcacgga
    33061 tcacttccat catgatgcga gcgctttccg gcgtcgcgtt cacagccact ttaccggtag
    33121 aggttttgat gaagtccgca cccgctttga tggagatttc agacgcttta cggatcagcg
    33181 cttcgtcttt cagttcgccg gtttcgatga tcactttcag cagtacattc gctgccgcgc
    33241 aagcctcttt acaggctttc accaggtcaa aaccaacctg ctcgtcaccc gccatcagcg
    33301 cgcggtacgg gaacaccacg tcaacttcat cagcaccgta ggcgattgcc gcacgggttt
    33361 ctgccagcgc gatttcgatg tcgtcgttac cgtgtgggaa gttggttacc gtagcgatac
    33421 ggatttccgg ggtgccctgc tctttcagag ttttgcgagc aatcgggata aaacgaggat
    33481 agatacagat agcggcggta ttgccgaccg gagttttggc ctgatgacac agagcaatta
    33541 ctttctcgtc ggtgtcgtcg tcattcaggg tggtcaggtc catcaatttc agtgcacgca
    33601 ggctgcttgc tttcagatca gtcatttcat tctcctggct tgtcgccaat aaaattcacc
    33661 ttgcgagttt gttagtattc taacatctac tccgcaacac acttcgatac acatcacaat
    33721 taaggaaatc tacttacaag tttgcatcac tgtaatgcga tctggttcaa ataattcact
    33781 ttcaaatgaa tgcgtcagtg gtggcaaacg catcaggatc aaagtgaaca tcacgaaact
    33841 tcttacaatg gcgcatcacc ttcggcataa aaggaaaaga agatgcccac ttctcatgaa
    33901 aatgcactgc aacaacgttg ccagcaaatt gtcaccagcc cagtgcttag cccggagcag
    33961 aagcgccatt ttctggcact ggaagcagaa aacaatctgc cttacccaca gcttcctgcc
    34021 gaagcccgcc gcgcgctgga tgaaggtgta atctgcgata tgtttgaagg tcatgcgccg
    34081 tacaaaccgc gctatgtctt acccgattac gctcgttttc tggcgaacgg ttccgaatgg
    34141 ctggagctgg aaggcgcgaa agatcttgat gacgcactct ctctgctgac cattctttac
    34201 caccacgtac cgtcggtcac atcgatgccg gtctacctgg ggcaactgga tgcgttgttg
    34261 caaccgtatg ttagaattct aacacaaaac gagatcgatg ttcgaataaa acgtttctgg
    34321 cgttacctcg acagaaccct gccagatgcc tttatgcacg ccaatatcgg cccgtctgat
    34381 tcgcccatta cccgcgcgat cttacgtgca gacgcgcagg tttcaccgaa cctgaccttt
    34441 atctacgatc ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt
    34501 gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg
    34561 ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg cagcacgctg
    34621 gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga cttctttacg
    34681 cgcactctac cgcactactg ccagcagcag atcgccatca tcgatgcgcg gtgtgaattc
    34741 ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg gctgattaac
    34801 cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt taacttgctg
    34861 tgtgagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt
    34921 tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa atatggctgg
    34981 caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg caccacgccg
    35041 ggcgcgcgtt tgccgtatgg cgatgagcca gatccgatca cccatctgca aactgtcgcc
    35101 ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga cgaaaccatc
    35161 aaacgtaacc cgcaggcact ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt
    35221 gaatttaccg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt
    35281 ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc
    35341 gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa
    35401 cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg ggccaggcag
    35461 tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg
    35521 gacgatggga cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt
    35581 gcagattgtt gacggcaaag tggtgtggaa cgctgtggtc tgcgagcaat gtgatacctg
    35641 cctgaagatg tgtccgcaac atgccacacc aatggcgcaa tccatgagcg tggacgaagt
    35701 gcttagccat gtgcgcaaag cggtgctgtt tattgaaggg ataacggtaa gcggcggcga
    35761 agccacgacc caactgccgt ttgtagtggc gctgtttact gctatcaaaa acgatccgca
    35821 actgcgccat ctcacctgtc tggtggacag taacggtatg ttgagcgaaa ccggctggga
    35881 aaaattactc ccggtgtgtg acggcgcaat gctcgatctc aaagcatggg gtagcgaatg
    35941 tcatcaacaa ttaaccggac gcgataatca acagattaag cgcagcatct atttgctggc
    36001 agagcgcggc aagctggcgg aactgcgttt gctgatgatc ccaggccagg tggattattt
    36061 gcaacacatc gaagaactgg cggcgtttat caagggactt ggtgatgttc cggttcgcct
    36121 gaacgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc
    36181 ggaagacgtt gagccgttgg ctgatgcatt aaaggtgcgc ggggtgagcc ggttgatatt
    36241 tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacag acttgtaggc
    36301 ctgataagac gcgtcaagcg tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg
    36361 aacgccttat ccggcctacg gttcggcaca aacctgtagg catgataaga cgcgttaagc
    36421 gtcgcatcag gcattatgcg ccgactgccg gatgctgcct acgaagggca aactacccac
    36481 gaacgttaaa caccgcatac gtgttattaa gcaacacttc ggcaatctca tccgcgggtt
    36541 ccgggcgtaa ctcgcaaagc acggcaaaca cacgggcagc ctgttccggg cggttaggct
    36601 gcccctgaaa accgttgagc ggcatatccg gcgcatcggt ttccagcaac aacgatgcca
    36661 gcggtaattt tgcgatgaca tcgcgggttt tactggcgcg tggataggtg atagtaccgc
    36721 ctacgccgat tttgtagccc agctgtacaa accgttcagc ctgttgctgg ctgccagaaa
    36781 atccgtggac aacaccagca cgcggcaaat cgtggtgttt aagatgtatc gccagtttgt
    36841 cgtgcgtgcg ccgtgaatgc aggatcaccg gcagatcgta gcgtttcgcc agtttcagtt
    36901 gttcgtcgag taaccactgc tgcctctcaa attgcggatc gtcgccgaag agatccagac
    36961 cgatctcccc caccgccacc accttcgccg gacgcctttc cagcgcctgc tgtagctgct
    37021 caagagacac atcgctatgt ttttccaaca taccaggatg caagcccaat gcggcataca
    37081 gcggttgata attttccgct aacgcctgca cgcgggcaaa attttccgcc tctgtcgccg
    37141 gaacaatgat cttgcctacg cccgcttgtg ccgcgcgttg caggctggcc tcttcatcgc
    37201 caataaaagg cgggaaatca aaatggcagt gagtgtcgat aaaacggcaa atcaagccag
    37261 atcctcattg ttaaatgtcg ggtcgttcgc ctgcggagcg tcgctcactt cggcaaccag
    37321 cgtatcgttg gcgactggcg caggcggaat gacaatcgat tccggcgtca ccactggcac
    37381 cagatggcgg gtaagcggcg ctttttcagt taatagcttg ccaaccgtgg cgaggaaata
    37441 acgaccgcat aaacgcccaa gtttatagtc ttcacgcagc gccggaatcc gactgccaag
    37501 cgcgatacta tgtaatggct tcggcggata aatttcggat atccgcagct tgcccggtgg
    37561 tttctcaata aattgctgaa tgtcacgata gctggtttca tgatgctgca ccagattgac
    37621 cagcggctgc aggctactgt cacccagcca gcgttccatg cgtttgaacc actgcggcgt
    37681 gtagtacatt tgtgacggca cagtgcggat gacgaccaac gttttagcgc cctgccttgc
    37741 cgcctcttta accggaatcg catcactgat cccgccatcc aggtagttaa tgccttccag
    37801 cgacactccg ctacgataaa agccaggtat cgcactggag gcgcgaatca catccagcca
    37861 gttttgtttg gttggtaaaa agtaattcgg cgcgtagtca tcctgacgac aggcgcacat
    37921 ataaaacgat ttgccgctgt caaacaaccg cgcggcggta tccatttgca acggcatctg
    37981 gcttgcggtg gcctccacca gccagtcgag atcgataaga tttcctccac ggacaaagcg
    38041 caatggatcg aaaaattcgc gttttgtggt atagcgcatg atgactttgc gcgcgtaacc
    38101 gggctgattg cagataaacg ccgagaggtt ctgcgccccg gcagatgtgc cgagataaag
    38161 atcgaaagga ttaaactgcg cgcgcataaa ctcatccagc acgccagccg tgaaaattcc
    38221 acgctgtccg ccgccttcgc acaccagagc tattcgtcca ggctggaacg gccttagcga
    38281 caacggcgca atattaccaa gcgttacagg tattcgctgc cccacttctg ccttcctgtt
    38341 tttgttgtga aagaaagaca aagtaacgca atttattatt cgctaaaacc gcaaaagcca
    38401 gtccgctgga ctggctttgg ataaaatttt cttaatggtc tgaaatctag ggtcgttttc
    38461 ggcccatgaa caaactcact aggaacagaa taatcccgac gacaaagaca attttagctg
    38521 cgcctgcagc ggtaccggcc agaccaccaa acccaagtgc ggcggcgatt aacgcgataa
    38581 ccagaaatat gatgccccaa cgaaacataa gcgtctcctt taccatagtt aatgtcaccg
    38641 cttgatatga gcgtgaaaat cactcaatgc gatattttta gtgtggtgca catcgcgcct
    38701 cccgacaaag ttcgggagga cgaattactt agttttcaga tcatttttaa cgcttttcac
    38761 accatctacc gctttggcga tactttcagc acggtcactt tgtgcctgag aatctacggt
    38821 accggagagc tgaaccacgc catcggtggt ttcaactttc actttacgtg aagggacgat
    38881 atcgtccgcc agcagtttgg ctttgatttc actggtggtg gcggtgtcac ccgcgtagcc
    38941 cttcaccgag ccttctttag cgtcgcgaac gtgcagtttg tcgctgacag aggtcacgcc
    39001 ttcaacgcct ttcgccactt tcactgcctc ttcagcctgg gcctgacttt caacgaaacc
    39061 gctcagggtc acgacttttt gatcggtttt gacagagata tcggtgctct tgatgttgtc
    39121 atgatccacc agcgccgcct tcactttcgc ggtgatggcg ctgtcatcca tgaaattacc
    39181 gactttattc atagagctat cgactttttg ccctgcgctt tcattggtag tctgcgcgtt
    39241 gttttccgcg taggcagagc cggtcgcgac ggcagaggtc aacattacag ccagcagagt
    39301 tttcgaaatc ttcagtcttg tcatagtcat cgatttattc ctgtatgttt gctcgtaatt
    39361 tgagcctagc aacacgaggt tgcattgctg aatagggaga gacttcaccc tctacagaag
    39421 tcaatggtcg ccatcacaaa agcgatgggt gatgaataac gaccattaca gcctctgaat
    39481 cagttattaa tatcggtaga atgacaatcg acggctttag atactgatat ctacgcattg
    39541 aacggtattt aacgccatca gaaatgtcat cactttgtta aatatagatc acaattttga
    39601 aaccgctcgg gatatcagcg aaaacataag caaaagtgaa tgttttaaga acattccgta
    39661 agcggctaat aaggaaggga aattgacagg gcgcagcggc taccgcgccc tggaggcaag
    39721 aattaatgct cgcgggtctg gtggaactga acgtccggat aacgttcctg tgccaggcgc
    39781 aggttgacca tgctggtagc gatgtaagcg aggttatcgc cgccatcaag cgccagttgg
    39841 ctttcgttct tacgcttgaa ctcttcgaat ttcttcgcgt ctgcacattc tacccagcgg
    39901 gcagtggcaa cgttgactga ctcatacact gcttcaacgt tgtattcgct cttcaggcgc
    39961 gctaccacca catcaaactg cagcacacca accgcaccaa cgatcagatc gttgttggag
    40021 attggacgga acacctgcac cgcgccctct tcggaaagct gtaccagccc tttgagcagc
    40081 tgtttttgct tcagcggatc tttcaggcgg atacgacgga acagttctgg tgcgaagttc
    40141 ggaataccgg tgaacttcat catctcaccc tgggtaaagg tgtcgccgat ctgaatggtg
    40201 ccgtggttgt gcaggccgag gatatcgccc ggatacgctt cttcaacgtg cgaacggtca
    40261 cccgccataa aggtcagcgc gtcggagatc accacatctt tcgcagtgcg cacctggcgc
    40321 agtttcatgc ctttttcata tttaccggac accacacgca taaacgccac gcggtcgcgg
    40381 tgtttcggat ccatgttggc ctgaatttta aatacgaagc cggtaaattt atcttcgctc
    40441 gcttctacgg tacgggtatc ggtctgacgc ggcatcggcg caggcgccca ctccaccagg
    40501 ccattcagca tatgatcaac gccgaagtta cccagcgcag taccgaagaa taccggagtg
    40561 atttcgcccg caaggaacag ctctttgtcg aactcgttag acgcgccttt aaccagttcc
    40621 agttcgtcac gcagctgctg tgccagatct tcaccaaccg cagcatcgag atccgggtta
    40681 ttcagccctt taacaatgcg gacttcctga atggtgtgcc ctttaccgct ctgatagaga
    40741 taggtttcat ctttataaag gtggtaaacg cctttaaaca gcttgccgca gccaattggc
    40801 caagtgatcg gtgcgcagcc aattttcagc tcgttctcaa cttcatcgag cagttccatc
    40861 gggtcgcgga tatcacggtc aagtttgttc ataaaggtga ggatcggcgt gtcgcgcaga
    40921 cgggtaactt ccatcagctt acgggtacga tcttcaacac cttttgcggc gtcgataacc
    40981 atcaggcagc agtccaccgc cgtcagggta cgataggtat cttccgagaa gtcttcgtgc
    41041 cccggggtgt cgagcaggtt aacaaggcaa tcgtgatacg gaaactgcat cacagacgta
    41101 gtaatggaga tcccacgctg cttttccatc tccatccagt ccgacttagc gtgctggttg
    41161 gaaccacggc cttttactgt accggcggtc tgaatggcct gtccgaacag cagcaccttc
    41221 tcggtgatgg tagttttacc ggcgtccggg tgagaaataa tggcaaaagt gcggcgcttc
    41281 gccacctctt tcaaataagg agacaacgtc ataattcttt cttcttgagt aagcggcatc
    41341 gcgccgcgct tgttgaaaac gaaaaattgc ggctatttta cccatcaacg ggggggaggc
    41401 aatcagtgtt tacacaggag ctgctccagt tcgtgcaacg aagaaacggt ccaggtgggc
    41461 gcgatgcctt ctggttgctc gcgattgtgc gcattcagcc agcaggtcgc aagcccggcg
    41521 ttgatgccac cgagaatatc ggactcggca gtgtcgccaa ccatcagcac gcgtgaacgg
    41581 tcaggattgc ccgcctgttc cagcgcataa tcgaaaattt tcttattcgg tttggcaacg
    41641 ccaacttctt cggaaatcac cagcaaatcg aagtaatcac gcaggcccgt ccgttccaga
    41701 cgcacttgct gcaaggcgct aaaaccgttg gtgatgatgc cgattttagc gttgctacga
    41761 atggcgttaa gcagagaaac cgcgcccggc aacggcgtac agatttccgc catcgcatta
    41821 ataaaggcat cgttaagcaa gcctgcttca actttcagcc gttcggccca gctctcgaaa
    41881 cgcccgtgct gaagctgtaa tgaagtgatc gcgccgtttt gataatccac ccacagtggc
    41941 ttgttaacgg cctgatagtc ctgaaaatct tcagcggtaa aggtgacgct gtaatcaaga
    42001 aacatccgct gcaggccggt gaatgagtca aaggtaaaca gcgtttcatc ggcatcaaag
    42061 aaaatccagt cccacttcat tattccacct tgtcttacat actgattggc aacgccatga
    42121 tgatggcgtc ttcgcgaccg tccgtggtgg ggtagtaatt gcggcgaatc gtcgcctcgt
    42181 taaagcctaa actttcgtac agggcaatgg cggcagcatt tgaggcacgg acttccagcc
    42241 atagtgtcgc cacgccgcgt ttttccagtt cgtcgatcag atgttccagc agcgccctac
    42301 ccagcccctg acgctgatag tcgggatcga ccgcaatatt gaacaatgta gcttcatcca
    42361 gcaccacttg cgtaatcgca aacgccgcca ttttgccgtt ttgcgttaac tgaaagttga
    42421 gataacgctc gccctggttg ctggcaaagg ttttttcact ccacggaaag gcgtgggcgc
    42481 gttgttcaat gtggtaagcc gctggtaaat cagtcgtttc gagggaagaa atcgtgttca
    42541 tatgtgcaaa tttgttgcca taacgcggcg cgtgccgttg ggtttgcccg taattcggtg
    42601 agcgccggtg atgccacctg agcgccttcc aatgatagcg gttcgtcagt acccaaccgc
    42661 cagctgttgc agcgactgcc ttgcggcagc atcgcgattt tttctggcgt cagttgcagc
    42721 acttgatcgg ggctgacggt taatgcgcgc agaacatcgc tcactaaagg atcagtcagg
    42781 gcgggaagat cgtttgccac catcaccaga cggacgtgtg ccgggctcgc aatggcaatc
    42841 tccccctgca acgcgccagg gcgacgcagc gaccactggg taatgcccag ttgctgtaac
    42901 tgccagtctc gtcgggatgt catagcgaat cgctcctgtt gtcaggggcg caaatatagc
    42961 aaattcgtcg ataccgcgcc aacatatggc tataatcgcc gccagtatca attgaggagc
    43021 attccatgtc tgcatttacc ccggcaagtg aagtcttgct gcgtcacagt gatgatttcg
    43081 aacaaagccg tattctgttt gccggagact tacaggatga cctgcccgcg cgtttagata
    43141 ccgcggccag ccgtgctcat acccagcaat tccaccattg gcaggtgtta agctgccaga
    43201 tgggggataa cgcccgtttt agtctggtcg ccacggcgaa tgatgtcgca gattgcgata
    43261 cgctgattta ctactggccg aagaacaaac cggaagccca gttccagttg atgaatttac
    43321 tttctctgct gccagtgggg acagatattt ttgtcgttgg cgagaaccgc agcggcgtgc
    43381 gcagcgctga gcagatgctg gcagattatg cgccgttgaa taaagtcgac agcgctcgtc
    43441 gctgtggcct ctattttggt cgtctggaaa aacagccagt atttgatgcg gataaattct
    43501 ggggcgaata cagcgtcgat ggcctgacgg tcaaaacgct gcctggcgtg tttagccgcg
    43561 acggtctgga tgtcggtagc cagttgctgc tctcgacgtt aactccgcac acgaaaggta
    43621 aagtgctgga tgtcggctgt ggcgcgggtg tactttcagt tgcctttgcg cgccattcgc
    43681 cgaaaattcg tctcaccttg tgcgatgtct ctgccccggc ggtagaagcc agccgcgcaa
    43741 cacttgcagc caacggtgtt gaaggtgaag tctttgccag caacgtcttt tccgaggtga
    43801 aaggttgttt tgatatgatc atctccaacc cgccgttcca cgatgggatg caaaccagcc
    43861 tggatgcggc gcaaacgctg attcgtggcg cggtgcgtca tcttaatagc ggcggcgagc
    43921 tgcgaattgt agcgaacgcc ttcctgcctt acccggacgt gctggatgag acatttggct
    43981 tccatgaagt tatcgcgcaa accgggcgct tcaaggtata tcgcgccatt atgacccgcc
    44041 aggcgaagaa aggttgagta tctgatatct gtcgccgcat accgtgtcgg atgcggcgtg
    44101 aacgccttat ccgacctaca aaacctgcac gttagccctt cgtaggccag ataagacgcg
    44161 ccagcgtcgc atctggcatc tgcccccgca tgcctaccaa acctgcacgc tagtttcctg
    44221 atggacattt ttccagcaat tacacatcag gcgataatta actattgacg aaaagctgaa
    44281 aaccactaga atgcgcctcc gtggtagcaa ttctttttaa gaattgatgg t
//
LOCUS       DAJVPP010000023        43420 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVPP010000023 DAJVPP010000000
VERSION     DAJVPP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.023"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            18..92
                     /locus_tag="OQM34_001245"
     tRNA            18..92
                     /locus_tag="OQM34_001245"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Gln,seq:ctg)
     gene            complement(246..1421)
                     /gene="ubiF"
                     /locus_tag="OQM34_001246"
     CDS             complement(246..1421)
                     /gene="ubiF"
                     /locus_tag="OQM34_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1451771.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1567..2991
                     /gene="miaB"
                     /locus_tag="OQM34_001247"
     CDS             1567..2991
                     /gene="miaB"
                     /locus_tag="OQM34_001247"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1451772.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3144..4184
                     /locus_tag="OQM34_001248"
     CDS             3144..4184
                     /locus_tag="OQM34_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1451773.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4181..4648
                     /gene="ybeY"
                     /locus_tag="OQM34_001249"
     CDS             4181..4648
                     /gene="ybeY"
                     /locus_tag="OQM34_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1451774.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4738..5616
                     /gene="corC"
                     /locus_tag="OQM34_001250"
     CDS             4738..5616
                     /gene="corC"
                     /locus_tag="OQM34_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1451775.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5641..7179
                     /gene="lnt"
                     /locus_tag="OQM34_001251"
     CDS             5641..7179
                     /gene="lnt"
                     /locus_tag="OQM34_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1451776.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTTLFGFAAVLMSLR
                     QRRK"
     gene            7576..8484
                     /gene="gltI"
                     /locus_tag="OQM34_001252"
     CDS             7576..8484
                     /gene="gltI"
                     /locus_tag="OQM34_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS1451777.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8654..9394
                     /gene="gltJ"
                     /locus_tag="OQM34_001253"
     CDS             8654..9394
                     /gene="gltJ"
                     /locus_tag="OQM34_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS1451778.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9394..10068
                     /gene="gltK"
                     /locus_tag="OQM34_001254"
     CDS             9394..10068
                     /gene="gltK"
                     /locus_tag="OQM34_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1451779.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10068..10793
                     /gene="gltL"
                     /locus_tag="OQM34_001255"
     CDS             10068..10793
                     /gene="gltL"
                     /locus_tag="OQM34_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS1451780.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10911..11846
                     /gene="rihA"
                     /locus_tag="OQM34_001256"
     CDS             10911..11846
                     /gene="rihA"
                     /locus_tag="OQM34_001256"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1451781.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11930..13600
                     /gene="hscC"
                     /locus_tag="OQM34_001257"
                     /pseudo
     CDS             11930..13600
                     /gene="hscC"
                     /locus_tag="OQM34_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13660..15111)
                     /gene="djlC"
                     /locus_tag="OQM34_001258"
     CDS             complement(13660..15111)
                     /gene="djlC"
                     /locus_tag="OQM34_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCS1451782.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QVGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15108..15815)
                     /locus_tag="OQM34_001259"
     CDS             complement(15108..15815)
                     /locus_tag="OQM34_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1451783.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15917..16471
                     /locus_tag="OQM34_001260"
     CDS             15917..16471
                     /locus_tag="OQM34_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1451784.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16481..17908)
                     /locus_tag="OQM34_001261"
     CDS             complement(16481..17908)
                     /locus_tag="OQM34_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1451785.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17905..18612)
                     /locus_tag="OQM34_001262"
     CDS             complement(17905..18612)
                     /locus_tag="OQM34_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1451786.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18776..19753
                     /locus_tag="OQM34_001263"
     CDS             18776..19753
                     /locus_tag="OQM34_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1451787.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19823..20305)
                     /locus_tag="OQM34_001264"
     CDS             complement(19823..20305)
                     /locus_tag="OQM34_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1451788.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20540..23122
                     /gene="leuS"
                     /locus_tag="OQM34_001265"
     CDS             20540..23122
                     /gene="leuS"
                     /locus_tag="OQM34_001265"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1451789.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23137..23718
                     /gene="lptE"
                     /locus_tag="OQM34_001266"
     CDS             23137..23718
                     /gene="lptE"
                     /locus_tag="OQM34_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1451790.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKDMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23718..24749
                     /gene="holA"
                     /locus_tag="OQM34_001267"
     CDS             23718..24749
                     /gene="holA"
                     /locus_tag="OQM34_001267"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1451791.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24751..25392
                     /gene="nadD"
                     /locus_tag="OQM34_001268"
     CDS             24751..25392
                     /gene="nadD"
                     /locus_tag="OQM34_001268"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1451792.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25416..26027
                     /locus_tag="OQM34_001269"
     CDS             25416..26027
                     /locus_tag="OQM34_001269"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCS1451793.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26287..26604
                     /gene="rsfS"
                     /locus_tag="OQM34_001270"
                     /gene_synonym="rsfA"
     CDS             26287..26604
                     /gene="rsfS"
                     /locus_tag="OQM34_001270"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1451794.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26608..27075
                     /gene="rlmH"
                     /locus_tag="OQM34_001271"
     CDS             26608..27075
                     /gene="rlmH"
                     /locus_tag="OQM34_001271"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1451795.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27106..29007
                     /gene="mrdA"
                     /locus_tag="OQM34_001272"
     CDS             27106..29007
                     /gene="mrdA"
                     /locus_tag="OQM34_001272"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1451796.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPYEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29010..30122
                     /gene="mrdB"
                     /locus_tag="OQM34_001273"
                     /gene_synonym="rodA"
     CDS             29010..30122
                     /gene="mrdB"
                     /locus_tag="OQM34_001273"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1451797.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30133..31221
                     /gene="rlpA"
                     /locus_tag="OQM34_001274"
     CDS             30133..31221
                     /gene="rlpA"
                     /locus_tag="OQM34_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1451798.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31360..32571
                     /gene="dacA"
                     /locus_tag="OQM34_001275"
     CDS             31360..32571
                     /gene="dacA"
                     /locus_tag="OQM34_001275"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1451799.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32682..32945
                     /locus_tag="OQM34_001276"
     CDS             32682..32945
                     /locus_tag="OQM34_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1451800.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33046..33687
                     /gene="lipB"
                     /locus_tag="OQM34_001277"
     CDS             33046..33687
                     /gene="lipB"
                     /locus_tag="OQM34_001277"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1451801.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33946..34899
                     /locus_tag="OQM34_001278"
     CDS             33946..34899
                     /locus_tag="OQM34_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1451802.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35108..36073
                     /gene="lipA"
                     /locus_tag="OQM34_001279"
     CDS             35108..36073
                     /gene="lipA"
                     /locus_tag="OQM34_001279"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1451803.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36174..36377)
                     /gene="tatE"
                     /locus_tag="OQM34_001280"
     CDS             complement(36174..36377)
                     /gene="tatE"
                     /locus_tag="OQM34_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1451804.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36506..37294)
                     /locus_tag="OQM34_001281"
     CDS             complement(36506..37294)
                     /locus_tag="OQM34_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCS1451805.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37387..37770
                     /gene="crcB"
                     /locus_tag="OQM34_001282"
     CDS             37387..37770
                     /gene="crcB"
                     /locus_tag="OQM34_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1451806.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37824..38033)
                     /gene="cspE"
                     /locus_tag="OQM34_001283"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37824..38033)
                     /gene="cspE"
                     /locus_tag="OQM34_001283"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1451807.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38208..38768)
                     /gene="pagP"
                     /locus_tag="OQM34_001284"
     CDS             complement(38208..38768)
                     /gene="pagP"
                     /locus_tag="OQM34_001284"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1451808.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39357..40742
                     /gene="dcuC"
                     /locus_tag="OQM34_001285"
     CDS             39357..40742
                     /gene="dcuC"
                     /locus_tag="OQM34_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1451809.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40783..41124)
                     /locus_tag="OQM34_001286"
                     /pseudo
     CDS             complement(40783..41124)
                     /locus_tag="OQM34_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41462..41971)
                     /locus_tag="OQM34_001287"
     CDS             complement(41462..41971)
                     /locus_tag="OQM34_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1451810.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42134..42379
                     /locus_tag="OQM34_001288"
     CDS             42134..42379
                     /locus_tag="OQM34_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1451811.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42354..>43420
                     /locus_tag="OQM34_001289"
     CDS             42354..>43420
                     /locus_tag="OQM34_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1451812.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAVREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRF"
BASE COUNT        10916 a        10889 c        10802 g        10813 t
ORIGIN      
        1 tcgtaagtgc acccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc
       61 attccgaggt tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt
      121 tttgcttttc tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg
      181 tcggatgcga cgctgccgcg tcttatccga cctacggctg gcacgcatcc ggcaatgttg
      241 taaggctaca accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct
      301 gccgccatta acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg
      361 gcataaaaca gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg
      421 taacgcttga ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga
      481 acatcaatca gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga
      541 tggatggtat gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca
      601 tgtcggcgcg tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac
      661 gggaaatgct tcgcgatttc cgcttggagt tgcgccatat tcatattctg caactggcga
      721 atacgcgccg gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac
      781 gcacgcggtc cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac
      841 tgtacgctaa tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc
      901 atctgccgca cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt
      961 tcaccgccct tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag
     1021 gttggcacac gtaacgttac tttcggatgc gcttccagcg cctgccacaa cgcctgttgc
     1081 aggactgtgt tttccaccat gtagccaagc aacggcaact ttaattcggc tgcgtcaaat
     1141 accacatgtg ccgtttccca ctcccaggtt tccagtctgc ggtaaggatg gcaacgcata
     1201 gcctgtactg catcccagac ccctaaccct ttaagcaatg ataccgaagc cgcgctgatc
     1261 gccgagatcc gcacgtccgg ttggctgtca gcgacaaacg gtgcaggttc tgcgtgctcg
     1321 atcaccgtta ccgaaaatcc gtgctgtgcc agccccagcg ccagtgcgcc gccgaccatt
     1381 cctccgccga caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc
     1441 aatatgcttt aagtttacag gatttttacc cccaagggct gataacgctc atactggtca
     1501 caacggcagc aaagcattac actatgcgcc ctgcattcct ggctactatt tcgcaagagc
     1561 aagtcgatga ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat
     1621 tcatcgaaga tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg
     1681 gaagaagcgg atgtgctact gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa
     1741 gtcttccatc agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc
     1801 ggcgtcggtg gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat
     1861 gtcgatatta tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg
     1921 cgcggcgacc gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt
     1981 ctgccggaac cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat
     2041 aaatattgca cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct
     2101 gacgatattc tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg
     2161 ctcggtcaga atgtgaatgc ctggcgtggt gagaactacg acggcaccac cggatcgttt
     2221 gccgatctgt tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc
     2281 agccatccga tcgaattcac cgacgatatc atcgaagtgt accgcgacac gcctgagctg
     2341 gtgagcttcc tgcatctgcc ggtacagagc ggttccgatc gcattctgaa cctgatggga
     2401 cgtacccata cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg
     2461 gatattcaga tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc
     2521 gagaaaacga tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc
     2581 tctgcacgtc caggaacgcc tgccgccgat atggttgatg atgttccgga agaagagaag
     2641 aagcagcgtc tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgt
     2701 cggatgctcg gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg
     2761 gagctttccg ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg
     2821 atcggtaaat tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa
     2881 gtggtgcgaa ctgaagatga aatgggtctg cgcatggcag aaacaccgga gtcggtgatt
     2941 gcccgcaccc gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc
     3001 ctgccgttcc ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg
     3061 cgcctttttc ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc
     3121 ggcatacaga taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag
     3181 cagacaatgc gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg
     3241 aacgccgtct cggcatcgag atcaatcgcc gcgataacca ctttaaactg accggccgtc
     3301 cgatttgcgt caccgctgcg gcagacattc tgcgtagcct gtatgtcgat actgccccga
     3361 tgcgcggtca gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc
     3421 gggtactgga gcaaagcgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa
     3481 ctaaacgcgg cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc
     3541 tcgaccatga cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag
     3601 tggctgcggc agttgatgcc ctggagcgtc aggaaattcg ccgtattctg ctgactcgtc
     3661 cggcggtcga agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag
     3721 acccgtatct gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagtcg
     3781 agaaactgat tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta
     3841 cgctgaacga cgcgtttatc attctcgatg agagccagaa cactaccatc gaacagatga
     3901 agatgttcct gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac
     3961 agatcgacct gccgcgtaat actaaatcag gcttacgtca tgccattgaa gtgctggccg
     4021 atgtcgaaga gatcagcttt aacttcttcc acagcgaaga cgtggttcgt cacccggtgg
     4081 tggcgcgtat cgttaacgct tatgaagcct gggaagaagc agaacaaaaa cgtaaagcgg
     4141 cgctggcagc agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga
     4201 tttacaactg gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg
     4261 gctgaatgcg gtgatcccgc agtttcagga agagtcggaa gtgactattc gcgtggtcga
     4321 taccgccgaa agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt
     4381 gctctccttc ccgtttgaag tgccgcctgg catggagatg tcgctactgg gcgatctggt
     4441 tatctgccgt caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca
     4501 ctgggcgcat atggtggtgc acggcagtct gcatttgtta ggttacgatc acattgaaga
     4561 tgacgaagca gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga
     4621 ggatccgtac attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg
     4681 ccgttggtga ttaacgacgg cgttttgatt aactaacatg agaacccata cgacgccatg
     4741 agcgacgaca attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg
     4801 ttactcagcc aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc
     4861 cgtgattccg ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg
     4921 atggacatcg cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc
     4981 ctgaaacgca accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca
     5041 cgtttcccgg tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac
     5101 ttgctgccgt ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag
     5161 gcggttgtcg ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag
     5221 cgttaccaca tggcgatcgt tattgacgaa ttcggtgggg tttccggtct ggtgaccatt
     5281 gaagacatcc tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat
     5341 atcgacttcc gtcagctgag tcgtcatacc tggaccgtgc gcgcactggc ttccattgaa
     5401 gacttcaacg aagcgttcgg cacccacttt agcgatgaag aagtcgacac tatcggtggt
     5461 ctggtgatgc aggcgtttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt
     5521 taccagttca aagtggcgat ggccgatagt cggcgtatta ttcaggttca tgtcaaaatc
     5581 ccggatgact caccccagcc aaagctggat gaataaaacc gaaactggat agataactac
     5641 atggcttttg cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc
     5701 ggtgcctgcg gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg
     5761 ctgatggggc ttcaggcgtt gacctttaac cgtcgcccac tccagtctgc cgctattggc
     5821 ttttgctggg gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg
     5881 acctttggcg ggatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat
     5941 ttgtcgctgt ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc
     6001 tggctgcgcg tggcgattgc cacccctgct ctctggcaag tgaccgagtt tctgcgcggt
     6061 tgggtgctga ctggcttccc gtggttacag ttcggctata gccagattga tggcccgtta
     6121 aaagggctgg caccgctaat gggcgtggaa gccattaact tcctgctgat gatggttagt
     6181 ggcctgctgg cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg
     6241 ctgtttgccc tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa
     6301 accattcagg tttcgatggt tcagggcgat attccgcaat cgctgaaatg ggacgaaggc
     6361 cagcttctta atacgctaaa gatttactac aacgcaacgg caccgctgat gggcaaatca
     6421 tcgttgatta tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc
     6481 ctcaaagcac tggacggtga gttgcgtgat aaaggtagct cgctggtaac cgggattgtc
     6541 gacgcgcgtc tcaataagca gaaccgctac gatacctaca acaccatcat cacgctgggt
     6601 aaaggtgcac cgtacagcta cgaatcagcc gatcgctata acaaaaacca tctggtgccg
     6661 tttggcgagt ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg
     6721 ccgatgtcgt cgttcagccg tgggccatat atccagccgc cgctgtcggc aaatggtatt
     6781 gagcttactg cggctatttg ctacgagatc attctcgccg agcaagtgcg cgataacttc
     6841 cgcccggata ccgactacct gctgactatc tccaacgatg cgtggtttgg taaatctatt
     6901 ggtccatggc aacacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg
     6961 ttgcgcagca ccaacaacgg cattacggcg gtgattggcc cgcagggtga gattcaggcg
     7021 atgatcccgc agttcgcccg cgaggtgtta accactaacg tgacgccgac caccggactc
     7081 acaccatacg cacgtaccgg caactggccg ctgtgggtgc tgacgacact gtttggtttt
     7141 gctgctgtgt tgatgagtct gcgtcagcga cgtaaataat cccttcttat cgtgccggaa
     7201 tacatcccat atttcggcac gctcaccgga tgcggcatca gtgcattatc cggataacaa
     7261 tattctccta tccaactttg gcacatctat tgctttgtta tacaaggcaa agcttgaacc
     7321 agcatcagta caacccggtt gcaccacagc aatccaccgg tagcaccaaa agagtgcatt
     7381 ttaagaattt tgcttcagaa tggtgcggtg cacctcgcaa aaacaaacaa taaaacatca
     7441 atatctttac atttagccaa actaaatgtt acctaacagg cacaacactg cacaataaag
     7501 ttgcagacga taacaacaca aacactcaca acgggtatcc atgcgttctt aacgcagaag
     7561 ataaaggtgt tggatatgca attacgtaaa cctgccacag caatcctcgc cctggcgctt
     7621 tccgcaggac tggcacaggc agatgacgcc gccccggcag caggcagtac gctggacaaa
     7681 atcgccaaaa acggtgtgat tgtcgtcggt caccgtgaat cttcagtacc tttctcttat
     7741 tacgacaatc agcaaaaagt ggtgggttac tcgcaggatt actccaacgc cattgttgaa
     7801 gcagtgaaaa agaaactcaa caaaccggac ttgcaggtaa aactgattcc gattacctca
     7861 caaaaccgta ttccactgct gcaaaacggc actttcgatt ttgaatgtgg ttctaccacc
     7921 aacaacgtcg aacgccaaaa acaggcggct ttctctgaca ctattttcgt ggtcggtacg
     7981 cgcctgttga ccaaaaaggg cggcgatatc aaagattttg ccgacctgaa aggcaaagcc
     8041 gtagtcgtca cttccggcac tacctctgaa gttttgctca acaaactgaa tgaagagcaa
     8101 aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg actctttccg caccctggaa
     8161 agcggtcgtg ccgttgcctt tatgatggat gacgctctgc tggccggtga acgtgcgaaa
     8221 gcgaagaaac cagacaactg ggatatcgtc ggcaagccgc agtctcagga agcctacggt
     8281 tgcatgttgc gtaaagacga tccgcagttc aaaaagctga tggatgacac tatcgctcag
     8341 gtgcagacct ccggtgaagc ggaaaaatgg tttgataaat ggttcaaaaa tccaattccg
     8401 ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa tgaaagcact gttcaaagaa
     8461 ccgaatgaca aggcactgaa ctaattacaa gaaccagggg cggaaattcc agccctctcg
     8521 attgttacgt agcacggaca gactatacgc ctgatggtcg ttccccatcg ggcctgaaaa
     8581 ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta acgctgctac cctctttttt
     8641 ctggagtaga tttatgtcta tagactggaa ctggggtatt tttttacaac aagccccgtt
     8701 cggcaatacc acctatcttg gttgggtctg gagtggtttt caggtgacga tcgctttgtc
     8761 gatctgcgcc tggattatcg ctttcctcgt cggttcattt ttcggcattt tacgtaccgt
     8821 tccaaaccgt tttctctccg gtctgggtac gttgtatgtc gaactgttcc gcaacgtgcc
     8881 gttgattgtg caattcttta cctggtatct ggtgatcccg gagctgctgc cggagaaaat
     8941 cggcatgtgg tttaaggccg agctggatcc caatattcag tttttccttt catccatgct
     9001 ctgcctgggg ctgtttaccg ccgcccgtgt ttgcgaacag gtacgcgccg ctattcagtc
     9061 gctaccgcgt gggcagaaga atgccgcgct ggcgatgggc ctgacgctac cgcagactta
     9121 tcgatacgta ctgctgccta atgcttatcg cgttatcgtc ccgccgatga cttccgagat
     9181 gatgaacctg gtgaaaaact ccgccatcgc ctcgaccatc ggtctggtgg atatggcggc
     9241 gcaggcgggt aaattgctcg actactcggc tcacgcctgg gagtcgttta ccgccatcac
     9301 gcttgcgtat gtgttgatta acgcttttat catgctggtg atgacgctgg ttgaacgtaa
     9361 agttcgcctg cccggcaaca tggggggcaa ataatgtacg agtttgactg gagttccatt
     9421 gttccttccc tgccatatct gctcgacggg ctggtgatca ccctgaaaat caccgtcacg
     9481 gcagtcgtga ttggtatttt gtggggtacc atgctggcgg tgatgcgctt gtccagcttc
     9541 gcgcctgttt cgtggtttgc caaagcctac gttaacgtat tccgctcgat tcctttagtc
     9601 atggttttgc tgtggtttta cttgattgtg ccgggatttc tgcaaaatgt gctgggatta
     9661 tcgccaaaaa atgatattcg tttaatttcg gcgatggtgg cgttttcgat gtttgaagcg
     9721 gcctactatt cagagattat ccgtgccggt attcaaagta tttcccgtgg tcagtcgagc
     9781 gccgcgctgg cgttggggat gactcactgg cagtcgatga aactgattat tctgccgcag
     9841 gctttccgcg cgatggtgcc gctgctgctc actcagggca tcgtactgtt ccaggatacc
     9901 tcgctggtgt atgtgttatc tctggccgat ttcttccgta ccgcctcaac cattggtgag
     9961 cgtgatggta cgcaggtcga gatgatcctg tttgccggat ttgtttattt cgttattagc
    10021 cttagcgcgt cgttgttggt cagctacttg aaaagaagga cagcataatg attaccctga
    10081 aaaatgtttc aaaatggtat ggtcactttc aggtgctgac cgactgctca accgaagtga
    10141 aaaaaggcga agtggtggtg gtttgcggcc cgtctggttc cggcaaatca acgctgatta
    10201 aaaccgtcaa cggcctcgaa ccagtgcagc aaggtgaaat caccgtcgat ggtatcgtgg
    10261 ttaacgacaa gaaaaccgat ctggcaaagc tgcgctcccg cgtcgggatg gtattccagc
    10321 atttcgagct gttccctcat ctgtcgatta tcgaaaacct gaccctggcg caggtgaaag
    10381 tgcttaaacg cgataaagcg cccgctcgtg aaaaagcatt gaaattgctg gagcgcgtgg
    10441 gactttccgc acatgccaat aaatttccgg cgcagctttc cggcggtcag cagcagcgcg
    10501 tggcaatcgc tcgcgcattg tgtatggctc ctattgcgat gctgttcgac gaaccgacat
    10561 cggcgctgga tccggagatg atcaacgaag tgctggacgt gatggtggaa ctggcgaacg
    10621 aaggaatgac catgatggtg gtgacccacg aaatgggctt tgcccgcaaa gtggcgaatc
    10681 gggtgatctt tatggacgag ggtaaaattg tcgaagactc gccgaaagac gctttcttcg
    10741 atgatccgaa atcggaccgc gcaaaagact tcctcgcgaa aatcctgcat taatcactct
    10801 ggcgcacata cccaattgcg cgccatctca cgctttatcg tttcgcccct cgtatctgtt
    10861 acatcctagc gttacccttg tcgcaaagaa gcacacaaca aggagcaaca atggcactgc
    10921 caattctgtt agattgcgac ccaggtcatg atgacgctat cgcaatagtt ctcgcactcg
    10981 cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc cggaaaccag acaccagaaa
    11041 aaaccttacg caatgttctg cgtatgctga ccttgcttaa tcgcaccgat attccggtgg
    11101 caggcggcgc ggtaaaaccg ttaatgcgtg agttgattat cgcggacaat gtgcacggcg
    11161 aaagcggtct cgacggcccg gcattaccgg aaccgacgtt cgcaccgcaa aactgtacgg
    11221 cggtggagct gatggcgaaa acgctgcgcg aaagtgcgga acctgtcacg attgtttcta
    11281 ccggaccgca aactaacgtt gccttgctgc tcaatagcca tccggaactg catagcaaaa
    11341 ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg taactggacg cctgcggctg
    11401 aatttaatat ttacgttgac ccggaagcgg cagaaattgt cttccagtca gggatcccgg
    11461 tggtgatggc cggtctggat gttactcata aagcgcaaat ccacgttgaa gacaccgagc
    11521 gtttccgcgc gattggcaac cctgtttcaa ccattgttgc cgaactgctg gatttcttcc
    11581 tcgaatatca taaagacgaa aaatggggct ttgtcggcgc accgctgcat gacccatgca
    11641 ccatcgcctg gctgttgaaa cccgagttgt ttaccactgt tgagcgctgg gttggcgtgg
    11701 aaacacaggg gaaatatacc cagggtatga cggttgttga ttattattat ctgactggca
    11761 ataaaccgaa tgccactgta attgtcgatg ttgatcgtca gggctttgtt gatttactgg
    11821 ctgatcgtct gaaattttac gcttaacact cgtcattgat taagatcacc acgcgttacc
    11881 tgggtggtct taatctattt atctcttttt ctttcaagat ataaatgcaa tggataatgc
    11941 agaactcgcc ataggtatcg atctcggcac taccaatagt ttaattgccg tctggaaaga
    12001 cggtgccgcg caattaattc caaataagtt cggtgaatat ttaacaccat ccataattag
    12061 catggatgaa aataatcata ttttagtcgg aaaaccggct gtatcacggc gtacttcgca
    12121 tccggataaa acggcagcgt tatttaaacg tgcaatgggc agtaatacca actggcggtt
    12181 aggcagcgac acatttaacg cgccagaact gtcctctttg gtattacgct cattaaaaga
    12241 agatgccgaa gaatttctgc aacgtccgat taaagatgtg gtgatctccg ttccggctta
    12301 tttcagcgat gaacaacgca agcatacccg tttagcagcg gacttagccg ggttaaatgc
    12361 ggtacgctta attaatgaac ccacagcagc tgcgatggcg tatggcctgc atacccaaca
    12421 aaatacccgt tcgctggtgt ttgatctcgg tggcggcacg tttgacgtta cggtgcttga
    12481 gtacgccacg ccggtgattg aagttcacgc ctccgctggc gacaacttcc tcggtggcga
    12541 agattttacc catatgttgg tcgatgacgt tttaaagcgc gcggatgtca caaggaccac
    12601 gcttaacgag agtgaactgg cagccttgta cgcctgtgtg gaagcggcaa aatgtagcaa
    12661 tcaatcgcca atgcacattc gctggtagta ccaggatgaa acgcgggaat gcgaatttta
    12721 cgagaacgaa ctggaagatt tgtggttgcc gctgctcaat cgcttgcgag tgccgattga
    12781 acaggcgttg cgcgatgcgc gtctgaagcc gagtcaaatc gacagtctgg tgctggttgg
    12841 cggcgcgtca caaatgccgc tggtgcagcg aatcgccgtg cgtctgtttg gcaaattacc
    12901 gtatcaaagt tacgatccga gcaccattgt cgcgctgggc gcagcaatcc aggccgcctg
    12961 ccgcttacgc agtgaagata ttgaagaagt gatcctcact gatatttgcc cttactcgtt
    13021 gggcgttgag gttaaccgcc agggcgtttc cggcattttc tcgccgatta ttgaacgaaa
    13081 caccactgtg cccgtgtcgc gtgtagaaac ttattcaacc atgcacccgg aacaggattc
    13141 aattacggtt aacgtctatc agggagaaaa ccacaaagtt aaaaacaaca ttctggtgga
    13201 atccttcgat gtgccgttga agaaaaccgg tgcttatcag tcgattgata ttcgctttag
    13261 ttatgatatc aacgggttgc ttgaagttga cgtgcttctg gaagacggca gcgttaagtc
    13321 cagagtgatt aaccacagcc cggtaacatt gagcgcgcag cagattgaag agagtcggac
    13381 gcggttatcc gcattgaaaa tttatccgcg cgatatgctc atcaatcgca cctttaaagc
    13441 caaactggaa gagttgtggg cgcgggcgct gggtgacgag cgagaagaga ttggccgggt
    13501 gatcaccgat tttgatgcgg cgctgcagtc aaacgatatg gcccgcgtcg atgaggtcag
    13561 gcggcgggcg agcgtttatt tagccattga gacctcataa tcacggctgc cggatgcggc
    13621 gtaaatgctg catccggcat cggcgcaaaa aacacatcgt caactaaaca acatcgcgat
    13681 tttgccgaga gcataaccta taaccagcac cgttaaactg attttgataa tgctggtaat
    13741 gctctgtggt ttttcaccct catcattttt ttcttcatgg ttttcaggtg ttggctcatc
    13801 gacatcttca tcctccggct cgagttttac tttcgtcaac agcatccggg tgataatggc
    13861 ctctaatgga tgagcatctc cgccttccag ccttgtatta agacgggcca gcatctcttg
    13921 tttcggaaac gcttcaagta ctacctgttc cgctacccga tacaaatcca gtaacggtga
    13981 atcttcttct gtctcgctac cgactctcga tttccaccac gcccatgctt tgcgtttgaa
    14041 gaataacgac tcaataaacg attgacctaa cggcgataat tttccgctat gtaaaatttc
    14101 cgcccataac agccgcgtcc gggcgctctc accaggaaca tatttcagcg gttgaccttg
    14161 cgcatctacc atgctccgcg cttcgataac catcactaat aacgcgaaat aatccgggca
    14221 atgacgcttg ctccactgca acaacaaatt atcagcttgt gtagatgggt aactttccca
    14281 gtacgcgccg agatcagcaa gcaggctttc cccctcgccg cagtacaaac gttgtgcaca
    14341 ctggtaatac cgggcatctt cgctttctgg ttcttccgct tgccactgtt tggctacagg
    14401 aatgagttcc accatacctg tttgcaccga gctgtaccag cgcaacagtt tgcgatgtaa
    14461 ctttgcatca tcaggaatga accacggtcc ttccatcgcc aaaaacgcag tgacatagtc
    14521 aggatggtaa cggcagcaac gttccagcgc gaagtaatat tcaacagtct ggttctgtaa
    14581 cgcgacggga tattccagaa ggctaaggaa atcgaaacaa tcaccaacct gaatggcctc
    14641 aagaaaagcc tccctttctt cctcatccat tccttcgctg tcattaaacg aatgccagtt
    14701 caaacgctct gccaacaaag aagcacaatt taatgacaga tatcgcgctt ctatagcgat
    14761 tgcacacagc ggccatcgta attgatcgac atcctccatg ttccaggtat ttaattgctg
    14821 aataaatttc tgccaggcgg agggctgaaa acgatcactt tctgaatcca gtaatgtcct
    14881 gaatgcacgt agtatttcat gttcagcggt ggcatctttt tcttcatcat ctgcttcctc
    14941 gacaggattt accgccagtc gcagcgcctc ttcataggcc tggcgtaatg ctttaaaccc
    15001 ctgcggatcg gtttcgggat gacacaacgg taagcgagca agataagcct ggcggataat
    15061 gtctatttgc gtcgtgcttt caatttcgag gatttgccaa catgttttca taggtcgctc
    15121 acatccttaa gcgttaccgg gcattcaggc tggacagtta atggctgcca gggaagcgtg
    15181 gcatagaaac ggtcgcttcc cgctgccagc tcttcgaaca tccgactccc ggcgctatcg
    15241 tatttgtaca gggttagcgc ctcgcgtagc gaagcgttgt cttcgcactg agaagattgc
    15301 cagtagagac gccctagtga ataggcagaa aaatagtgca tccagctgtg atagtaatgg
    15361 tgagctcgtg catagacgcg cgattgcagc cataaactct cttcttccgt cagccagttg
    15421 ttgagcacac cggcccgtga cagaaacccc attcgaacat aatcccaggc tttgattccc
    15481 ccttctccac agcacatagc ggtttgggca acgaattggg tataaatcaa ccctctctcc
    15541 gagccaccag caattaatgc tttccactct tcaggggaag agcgttgcca tgtcagatat
    15601 aagccagcta aatcattggc atgaccatca tcagtcatct gataaatagt ctgaattaaa
    15661 tcatcacgat tatttattcc ccaggatgat gagagatcga attcttttat cccggtatac
    15721 atttttggcg agtcatgcgc gggtttgcaa ctctgattaa gtatttccat gggcgcagaa
    15781 agcgcaaaaa gaagatattg ttcttcctta ttcatttttt aataatccct taagtgcatt
    15841 aactgactac ttttttatta ttgatgagta taaacttgac gctttcaaaa taaaaagaaa
    15901 atcgaagcat tcacacatga ataaaaaatt aatgtatata ttcgcaattt ttatagttgc
    15961 agcaattacc tgtattagcc aacccaagaa aacgatgttg cgtgataaag ccatggtgaa
    16021 ttatgccttt gattatttaa gatcaccagg cagtctaccg ttaaccacgg cagccacgga
    16081 gctttccgcg attcatggtc actcaacatc gcaatatcgc cttggcgaat tttatcttca
    16141 tggtagcgac ggtaaaccac tggattatac acaggcgaga tactggtatg agcaatcagc
    16201 agaacaggaa aatccgcgcg cacaaagcaa actggggtgg atctacctga aaggcctggg
    16261 agtcaaaccc gatacccgta aagcaattct ctggtataag gaagcagctg aacaagggta
    16321 tgctcatgct caatatactt tgggtttgat ctacagaaat ggcactggta ttaatgttaa
    16381 ccattatgaa tctcaaaaat ggttaaaact ggccgccaaa caacattaca aaaatgcgga
    16441 aagattactt gccggacttc ccgcacatta acgtcgactg tcacccgaag agatggagta
    16501 ttttccctat caaaccggca aagataaaaa tgtagaaaat aattttgatg acgcccagac
    16561 agcccagccc gccatcatca acgggcattg gctgcggttg cggctccggc tcgacggttt
    16621 cctgaccggt aaaattcgcc gcctgaatta ataatcgacg aataattaat gcttccagtg
    16681 gacaacgatc ttctgcatca aggcggctca caatcggcat taatgattct tctgtcggtt
    16741 ggctggcctg cactaattgt ttggcaacta aatataatgg ccataaggga tgttctgtat
    16801 cacgcgtgag tgagatccgc ggcttacgtc ctttgtgcaa aaccatctcg accagtgaac
    16861 gccccagcgc agagagtttt tcatcgctga gtatttcgta taaatgaaaa cgcgtacggg
    16921 cgctgtcgcc ggggacataa agtagcggtt ttccctgatc gttaaccaga tcacgcgctt
    16981 caatcatcat caccactaat gggtaataat agacccgatg ctgacggcac cattgcaaca
    17041 ttaaagcatc cgcctgggtg gagggatact cgcgccagta gtaacaaagt tctgccagca
    17101 ggctttcgcc ttctccacaa tagacgcgtt gtgcgtattc ataataacgc gcatcttcat
    17161 tatcaggttc agcttcccgc cacgaccggg cgtaatccag gagttcgggg attcccaggc
    17221 ggcttgcgct ataccagcgg agtaaatttc tgtgcagttt tgcatcatcg gggataatca
    17281 ctgcgcgatg ttgcgctaaa tacaatgtca gccattctgg atgcgatgac caaatccgag
    17341 cgaacatttg gtaaaaatcg atggtgtcat tttgcacggc aactggcaga tgcagaatgg
    17401 tctggaagtt aaaaacattc cccttcgcaa tggcatataa aaaggattcc agttcttctt
    17461 cgtctatttc cccgacgttt tcctcctgca accaccgcaa tctttctgct aataacacca
    17521 cgcactcgaa ggataaatgg gcagtgttca tggcgattgt gcacagcgac cagcgtaatt
    17581 catcaatctc ttccatcgag caataattta attgctgaat gaatcgctgc caggcggagg
    17641 acagaaaacg ttcgctatca gaggcaagta acgcgcgaaa ggcgagcaga atttcatgtt
    17701 ctgctacctc atattcttct ggttgccaaa cagatttagc aggcgactgc gcaatccgta
    17761 gcgcctcctc atacgcctga cgaagttgtt taaaaccctg cggatcggtt tctggatgaa
    17821 aggacggtaa cagcgccaga taagcgcggc ggataatatc gacgtcagtc gtttcctcta
    17881 tatcgaggat cttccagcaa tttttcataa gtcgctcata tccttaagcg tttccgggca
    17941 tgtggggtag tcagccagtg gttgccagga caaggtaggg taatacgcat catcactggc
    18001 aaataattga taaaacatat cattgccgga atcgtcgtat tctttacgcg ctaagaattc
    18061 gcgtaataaa ggcagatgat catcttcggg agactgccaa tattgccgac caaaggtata
    18121 accggcaaag tattgccgcc agttactata ataacgtaga gcccgtagat gaatacgcga
    18181 ctggatccat agactttctt cttcactcaa ccagttatta agtacgccca ttcgacttaa
    18241 aaaacccatc cggacataat cccatgcttt aattcctccc tcaccacagc attcggctgt
    18301 gctggcgaca aattgcgcat aagcctgacc ttgctcattt aattcagcaa gatagtcacg
    18361 ccattcacat ggagaataac gaaaccagcg gtgataaaac cctgccaacc gagcggcatg
    18421 accgttctcg atcattcggt gaattatgcg cagcaggtca tcacggtcat caattcccca
    18481 tgaggaagaa aggtcaaaaa agcatgtacc taaatacatt ttgggggatg aatgcgaagg
    18541 taaacagcat tcattgcgta tttccatcgg agtggaaagt gcaaacaata ttttttgcga
    18601 ccccatgtcc atataattta tccttaaaca ttcatttaat gaatgtaaac tacatttcga
    18661 ggctcagtta tttatacttt tctgacgcaa cataatacgc ataaaataag gcttacgcaa
    18721 aaatggaata cataaaaata taatatattt tatctacaac cataaggaaa tgataatgat
    18781 ttttacgtca agttgctgcg ataatttatc aatagatgag attatcgaac gtgctgaaaa
    18841 aggcgattgc gaggctcaat atattgttgg gttttattat aatcgcgata gcgcaattga
    18901 ctctccggac gacgaaaagg ccttttactg gctgaagctg gccgctgagc aaggtcattg
    18961 tgaagcacag tattccttag ggcaaaagta taccgaggat aaaagccgtc ataaagataa
    19021 tgagcaagcc atcttctggc tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa
    19081 cgcccttggc tggatactgg atcgtggaga agaccccaac tataaagaag cggttacctg
    19141 gtatcagata gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg gatggatgta
    19201 cagaaatggc aacggagtcg cacaagacta tacgctggca tttttctggt acaaacaagc
    19261 tgcattacaa ggccatagtt acgcacaaga caatctggcc gatctttata aagacggaga
    19321 aggcgttgct caaaacaaga cactcgccgc attctggtat ttgaaaagcg cacagcaggg
    19381 taatcggcac gcccagtttc aaattgcatg ggattataac gctggcgaag gggtggacca
    19441 ggactataag caagcgatgt actggtatct gaaggctgcc gctcaggaaa gcgtcggcgc
    19501 ttacgtcaac atcggttata tgtataaaca cggacaaggc gttgagaaag attatcaggc
    19561 tgcctttgaa tggtttacga aagccgctga atgcaatgac gccactgcct ggtataacct
    19621 ggccattatg tatcactacg gagaaggaag acctgtcgat ctccgacagg ctctcgacct
    19681 gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt caggaaattc gtgagactga
    19741 agctttactg tagaaataaa aacaagggcc ggggaatttc attctgccca tattctcttt
    19801 attgctgtgc gaaatcagac tattacggct caaacgggcg tctctggaac tggtcataac
    19861 cacatttcgg gcatagcgtc agcacttccg gtgtgtagat cgggagatgg aagtgacatt
    19921 tctcgcagac cagatttccc agcccgacca cttctccgct gtgataaacc ccatgatgat
    19981 tgaggtcctg gaaaatttcg cgccattcaa gctgcgtttt atcggtgatg tctgccagct
    20041 cctgccacaa gctttcttta atcacccgca taaagacgct gtcagattct tctttcaggc
    20101 tctcttcata gctcatggcg aactcttcca ggtcacgtct gacagctcgc gtcagctcat
    20161 cgatctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca
    20221 gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgtt cagtgacgcg accagttcac
    20281 ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata
    20341 atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtac
    20401 gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt
    20461 tgggctatgc tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa
    20521 aacaggacca ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt
    20581 acagcttcat tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa
    20641 gtattactgc ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg
    20701 taactacacc atcggtgacg tgatcgcccg ttaccagcgc atgctgggca aaaacgttct
    20761 gcagccgatc ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa
    20821 caccgctccg gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat
    20881 gctgggcttt ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg
    20941 ttgggaacag aaattcttca ccgagctgta taaaaaaggc ctggtatata aaaagacttc
    21001 tgcggtcaac tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg
    21061 ctgctgctgg cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa
    21121 aatcactgct tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga
    21181 caccgttaaa accatgcagc gtaactggat cggtcgttcc gaaggcgtgg agatcacctt
    21241 caacgttaac gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat
    21301 gggttgtacc tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa
    21361 taatcctgaa ctggcggcct ttattgacga atgccgtaac accaaagttg ccgaagctga
    21421 aatggcgacg atggagaaaa aaggcgtcga tactggcttt aaagctgttc acccattaac
    21481 gggcgaagaa attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc
    21541 agttatggcg gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct
    21601 gaacatcaaa ccggttatcc tggcggctga cggctctgag ccagatcttt ctcagcaagc
    21661 cctgactgaa aaaggcgtgc tgttcaactc tggcgagttc aacggtcttg accatgaagc
    21721 ggccttcaac gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa
    21781 ctaccgcctg cgcgactggg gtgtttctcg ccagcgttac tggggcgcgc cgattccgat
    21841 ggtgacgctg gaagacggta ccgtaatgcc aaccccggac gaccagctgc cggtgatcct
    21901 gccggaagat gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc
    21961 aaaaactacc gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat
    22021 ggagtcctcc tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga
    22081 ttccgaagcg gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc
    22141 cattatgcac ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt
    22201 gaactctgac gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt
    22261 ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga
    22321 acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta
    22381 taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt
    22441 tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat
    22501 gactctcgaa tggcaggaat ccggtgtgga aggggctaac cgcttcctga aacgtgtctg
    22561 gaaactggtg tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct
    22621 gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa actatcgcta aagtgaccga
    22681 tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa
    22741 caaactggcg aaagcaccga ccgatggcga gcaggatcgc gctctgatgc aggaagcact
    22801 gcaggccgtt gtccgtatgc tcaacccgtt caccccgcac atctgcttca cgctgtggca
    22861 ggaactcaaa ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc
    22921 gatggtggaa gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat
    22981 caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct
    23041 ggtagcaaaa tatcttgatg gcgttactgt acgtaaagtg atttacgtac caggtaaact
    23101 cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacactgttg
    23161 ttatctctgg cggtgttaat caccgccggg tgtggctggc atctgcgtga caccacgcag
    23221 gttccttcca ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc
    23281 cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg
    23341 cgtaaggacg ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg
    23401 gtattccgta acggtcaaac agcagagtat cagatgatca tgacggtgaa tgcgaccgtg
    23461 ttgatccccg gccgtgatat ctacccgatt agcgccaaag tcttccgttc attcttcgat
    23521 aacccgcaaa tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agatatgtac
    23581 gaccgtgctg ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt
    23641 tccgacgaag aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctcc
    23701 accatgctgg gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg
    23761 aagggctgcg cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc
    23821 aggacgctgt tcgccaggta gctgcggcac aaggattcga agaacaccac actttttcca
    23881 ttgatcccaa cactgactgg aatgcgatct tttcgttatg ccaggctatg agtctgtttg
    23941 ccagtcgaca aacgctattg ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg
    24001 aacaacttct cacactcacc ggacttctgc atgacgacct gctattgatc gtccgcggta
    24061 ataaattaag caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg
    24121 tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgtgcgcg
    24181 caaaacagct caacttagaa ctggatgacg cggcgaatca ggtgctctgc tactgttatg
    24241 aaggtaacct gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg
    24301 gcaaactgac attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt
    24361 ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc
    24421 aactgcgtct ggaaggcagc gagccggtta ttttgttgcg cacattacaa cgtgaactgt
    24481 tgttactggt taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata
    24541 agcatcgggt atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagtc
    24601 agccgcagtt acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag
    24661 attacggtca gtcagtgtgg gcagagctgg aagggttatc tcttctgttg tgccataaac
    24721 ccctggcgga cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc
    24781 acctttgatc cggtgcacta tggtcatcta aaacccgtgg aaacgctggc gaatttgatt
    24841 ggtctgacgc gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa
    24901 gcgaacagcg tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt
    24961 actcttgatg aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa
    25021 gagtggcggc aggaacaagg accggacgtg ccgctggcgt ttattattgg tcaggattca
    25081 ttgctgacct ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc
    25141 gtctgtcggc gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg
    25201 gaagatcatt tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat
    25261 ctggctgaaa cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac
    25321 ggtgaatcgt gtgaggattt attgccggaa ccggtattga cttacattaa ccaacaaggc
    25381 ttgtatcgct gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg
    25441 gcgaaacaca agcgaatgtc gatggtctgt acagcggtca tgcgcccaca cccctgaccg
    25501 cacgcggtat tgagcaagcg caaaatctgc atacactgct agatgatgtt tcctttgacc
    25561 tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt ctcagtgacc
    25621 gccagctccc cgttcatatc atccctgaac tcaacgaaat gttttttggc gactgggaga
    25681 tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg
    25741 actggcagca tgctatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg
    25801 aacgctttat cgcaaggctt agtgaatatc agcactatca gaatatttta atcgtcagcc
    25861 atcagggcgt actgagtctg ttaattgctc gtttaattgg catgcctgcc gagtctatgt
    25921 ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa aaattcgcga
    25981 cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa cgcatgactt ttctgttttt
    26041 ttacgggtaa gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca
    26101 gactttttcc gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct
    26161 gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga
    26221 aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg
    26281 gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa
    26341 ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga ctgcatgatc
    26401 atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag
    26461 tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt
    26521 gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat
    26581 gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa
    26641 atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg
    26701 cccttcgagc tgattgaaat tccggccggc aaacgcggca agaatgcgga catcaagcgc
    26761 atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc
    26821 ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg
    26881 aagctggatg gtcgcgacgt aagtctactg attggcgggc ctgaagggtt gtcgcctgcc
    26941 tgtaaagcgg cggctgagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg
    27001 gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct
    27061 tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac
    27121 tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt
    27181 ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt
    27241 cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg
    27301 cccagccgcg gcattatcta cgaccgtaac ggtatccctc tggccctcaa ccgcactatc
    27361 taccagatag aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct ggacgctttg
    27421 cgcagcgtgg tagatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt
    27481 tcacaccgtt tcacctctat tccggtgaaa actaacctaa ccgaagtaca agtagctcgc
    27541 tttgccgtca atcagtaccg ttttccgggt gtcgaagtta aaggctataa acgtcgttac
    27601 tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa
    27661 gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatt
    27721 ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa
    27781 gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt accaccgcaa
    27841 gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg
    27901 ctggcgggta gccgcgcagc tgtggttgtt accgaaccgc gtacaggtgg ggtgctggcg
    27961 ctggtttcca cgcctagtta tgacccaaac ctgtttgttg acggtatctc cagcaaagat
    28021 tattccgcct tgctgaacga cccgaataca ccgctggtga accgcgccac acagggggtt
    28081 tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattaag cgccggggtg
    28141 atcacgcgca atacgacgct gtttgaccca ggctggtggc aactaccagg gtcggaaaaa
    28201 cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa
    28261 gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc
    28321 gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt
    28381 tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag
    28441 ggtgacacca ttccggttgg tatcggccag ggttactgga ccgcgacccc aatccagatg
    28501 agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg
    28561 agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgtatgaacc gcccgtcggc
    28621 gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc
    28681 cctaatggta cggcgaataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatct
    28741 ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc
    28801 gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg
    28861 gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac gctgatgcgc
    28921 cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcca
    28981 gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct
    29041 gggataaagt ccatctcgat cccacaatgc tgctgatttt actggcattg ctggtttaca
    29101 gcgccctggt tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg
    29161 gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt
    29221 atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg
    29281 ctttcggagc catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc
    29341 agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg
    29401 acgtttgccc gccatcgttg aagaatactg gcatcgcgct ggtgctgata tttatgccca
    29461 cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc
    29521 tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg
    29581 tagcggcgtt cattccgatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg
    29641 taatgatgct cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt
    29701 ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcac ggcactcagt
    29761 cacagcttga atttctcccc gaacgccata ctgactttat cttcgcggta ctggcggaag
    29821 agctgggatt agtgggcatt ctgattctgc tcgctctcta cattctgctg atcatgcgcg
    29881 ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggtggcttaa
    29941 tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg
    30001 ttgtaggggt tccgctccca ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg
    30061 ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt
    30121 aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg
    30181 gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt
    30241 aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg
    30301 gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga
    30361 tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc
    30421 tctggcgaag ctttcgatcc gacaaagctg acggcggccc atccaacgct tccgatcccc
    30481 agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat
    30541 cgcggccctt acggcaacga ccgcgttatt tcgctttctc gcgcggcagc tgaccgtctt
    30601 aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg
    30661 ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca
    30721 cctcccgatt taagcggtgg cgcgggaaca agttcagtgt ctggcccgca gggtgacatt
    30781 cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt
    30841 agcggtttcc tcggcgctcc aacgacctta gcgcctagtg tactggaagg cagcgaaccg
    30901 acgcctgcgc cacagcccgt tgttacagct tcgtcgacaa cgcctgcaac ctcgcctgca
    30961 atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg
    31021 gccgtaagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc
    31081 gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg cccattcgcc
    31141 agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca
    31201 tttattacta ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga
    31261 cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg
    31321 cactttttta attccatcac ggatgtcgta gttcagacca tgaataccat tttttccgct
    31381 cgtatcatga agcgcctggc gctcaccacg gctctttgca cagcctttat ctctgctgca
    31441 catgccgatg acctgaatat caaaactatg atcccgggtg taccgcagat cgatgcggag
    31501 tcctacatcc tgattgacta taactccggc aaagtgctcg ccgaacagaa cgcagatgtc
    31561 cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt acgttatcgg ccaggcaatg
    31621 aaagccggta aatttaaaga aactgattta gtcactatcg gcaacgacgc atgggccacc
    31681 ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca aaccgggcat gcaggttccg
    31741 gtttctcagc tgatccgcgg tattaacctg caatcgggta acgatgcttg tgtcgccatg
    31801 gccgattttg ccgctggtag ccaggacgct tttgttggct tgatgaacag ctacgttaac
    31861 gcactgggcc tgaaaaatac ccacttccag acggtacatg gtctggatgc tgatggtcag
    31921 tacagctccg cgcgagatat ggcgctgatc ggccaggcat tgatccgtga cgtaccgaat
    31981 gaatactcga tctataaaga aaaagaattt acgtttaacg gtattcgcca gctgaaccgt
    32041 aacggcctgt tatgggataa cagcctgaat gtcgacggca tcaaaaccgg acacactgac
    32101 aaagcaggtt acaatcttgt tgcttctgcg actgaaggcc agatgcgctt gatttctgcg
    32161 gtgatgggtg gacgtacttt taaaggccgt gaagccgaaa gtaaaaaact gctaacctgg
    32221 ggcttccgtt tcttcgaaac cgttaaccca ctgaaagtag gtaaagagtt cgcctctgaa
    32281 ccggtttggt ttagtgattc tgatcgcgct tcgttagggg ttgataaaga cgtgtacctg
    32341 accattccgc gtggtcgcat gaaagatctg aaagccagct atgtgctgaa cagcagtgaa
    32401 ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta tcaacttcca gcttgatggc
    32461 aaaacgatcg agcaacgccc gctggttgtg ttgcaagaaa tcccggaagg taacttcttc
    32521 ggcaaaatca ttgattacat taaattaatg ttccatcact ggtttggtta aaaatcgaac
    32581 acttgaaagt gtaatttccg tccccatata ctaagcatca gtaaaaaaac tcccgccttc
    32641 tggcgggagt tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg
    32701 aactgcttga attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc
    32761 tggttgatca ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg
    32821 taaaaccaag cagcaaaggc aactaccact cggtatctat cactatcaac gccactcata
    32881 tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc
    32941 tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa ctcccctctc ctcgctgtga
    33001 tatacttttc ccccactttt actcattctc cacggagatg ccgttttgta tcaggataaa
    33061 attcttgtcc gccagctcgg tcttcagcct tacgagccaa tctcccgggc tatgcatgaa
    33121 ttcaccgata cccgcgataa tagtaccctt gatgaaatct ggctggtcga gcactatccg
    33181 gtattcaccc aaggtcaggc aggaaaagcg gagcacattt taatgccggg tgatattccg
    33241 gtgatccaga gcgatcgcgg tgggcaggtg acttatcacg ggccgggaca acaggtgatg
    33301 tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt
    33361 gagcaaacag tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac
    33421 gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg aattcgacgc
    33481 ggttgttcat tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt
    33541 attaatcctt gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa
    33601 gcgacgacta ataatattgc tccacgttta ctggaaaata ttttagcgct actaaacaat
    33661 ccggacttcg aatatattac cgcttaattc catacatcaa tggcccaatt tacactgggt
    33721 cattactcat aatacctgct aatgctttac atctccttcg cgtttgtata ttttctttat
    33781 cacttgcaga aatgcggtgc cacgccttgc tccgtaactc accccattga ttaacctatt
    33841 gaaatagttg attatggtga gcaaaagcgt tccgttccaa ttagacataa gattattgta
    33901 attttttaaa tattattttt ccatgaataa aattggagta agtgcgtgga tagtaataat
    33961 caaattgaac cctgtttaag cagaaagtcg tcagaaggta aaccacaaat atttacaacc
    34021 ttacgaaata ttgatcttaa ccttctgact atttttgaag ctgtatatgt acataaaggg
    34081 atcgttaatg cagcgaaagt gcttaatctg accccctcgg caatcagtca gtctattcag
    34141 aaactgcgcg ttatattccc tgacccattg tttattcgca aaggccaggg tgtcactcct
    34201 accgcatttg cgatgcatct acatgagtat atcagtcagg gccttgagtc cattcttggc
    34261 gcgctggata tcgaaggaag ctatgataag caacgaacga taactattgc gaccactccc
    34321 tcggtcggag ccctggtcct tcctgtcatc tatcgggcga ttaaaactca ctatccgcag
    34381 cttttactgc gcaacccgcc catcagcgac gcggaaaacc aactcagtca gtttcaaacc
    34441 gatctcatca tcgataacat gttttgcacc aatcgtacgg tgcaacatca tgttctgttc
    34501 accgacaata tggtgttaat ttgccgcaag ggaaatccac tactctcttt agaagatgac
    34561 agagagacta tcgacaacgc tgcgcatgta cttctgttac cggaagggca aaatttcagc
    34621 ggtctgcggc agagagttca agagatgttt ccggaccggc aaatcaattt caccagctac
    34681 aacattttga caatcgctgc actggttgcc aacagtgaca tgttagcgat tattcccagc
    34741 cgtttttata acctgtttag tcgttgctgg ccgctggaaa aactgccttt tccgtcctta
    34801 aatgaggagc aaatagattt ctctatccac tacaacaaat tcatcctgcg tgatcccatc
    34861 ctgcacggag taatagatgt tatccgtaat gcattttgaa aaagtattgt tacagccaga
    34921 tcacagcgca gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc
    34981 tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt
    35041 gataattaca acaatctatt gaattgaaac gctttccttc gtaattcgca actggaacac
    35101 gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa
    35161 gatggccctt atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc
    35221 ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagccgc
    35281 aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga
    35341 atgcttcaac cacggcacag caacgtttat gatcctcggc gctatttgta cccgccgttg
    35401 tccgttctgt gatgttgccc acggtcgccc ggtagctcct gatgccaatg aaccagtgaa
    35461 actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg
    35521 tgatgacctg cgcgatggcg gtgcccaaca ctttgcggat tgtattactg ccattcggga
    35581 aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgcggtc gaatggatcg
    35641 tgctctggat attctgactg caacgccccc agatgtgttc aaccataacc tggaaaacgt
    35701 accgcgtatt taccggcagg tacggcctgg cgcagattac aactggtcgc tgaagctgct
    35761 ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtggggct
    35821 gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac
    35881 gatgttaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta
    35941 cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca
    36001 tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaaggaat
    36061 ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg
    36121 aagcgggttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct
    36181 ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg
    36241 tcatcattca tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta
    36301 cgtaacttct tagtcccaaa cagcagaacg accagcgccg caactaccag cagtttggta
    36361 atactaatct cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa
    36421 aaaccagaga atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt
    36481 gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt
    36541 ttaagatggg cagttgcgcg cgcacctgac gcacacgttc gggcgtcact tccgccataa
    36601 tgagtgcagg catttctgac gctgccgcaa tggtgacgcc aaagggatct ataatccggc
    36661 tttgcccgat atttttgttc ccgcactccc ccgccgccac catataacag gtggtatcca
    36721 gcgcacgagc ggcaagcaac gttgaccagt gatgctcttt gagcggccca cgaacccagg
    36781 cggcaggaag taccaggatt tcagctccct gtaaagcctg tgccagcgcc agctctggaa
    36841 agcgtaagtc ataacaggtc atcagaccga ccttcatccc ttccacctcc agtaacggag
    36901 ctatttcatt accagcatca acatggcgtg attcctgaat ggcaaatgca tcatagagat
    36961 gcagtttggc ataacgggcg acgatgtttc ctgcctgaag tgccaccagc atattccatg
    37021 cgcgccccgg cgttgaagga agatgaatcg tcagaattgt cgtcatcatg ttacgtttac
    37081 tttctcgccg taaacgtccg aggaattcgc cttccagcag ctgtgctgat ttaaccgata
    37141 gatctgcatc atgatcatcg cgcgccagca atgcttccgg caggacaaac agcgatacgt
    37201 cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag
    37261 atgtaacagc aaactgtcct gcagcaacca gcattgccac ctcctgtcag agtcgtaaaa
    37321 ttcgccaacg acgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat
    37381 tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtagcg
    37441 agatggctgt taagtatgcg atttaacccg ctgcatcagg cgattccgtt ggggacgctg
    37501 gcagcaaacc tgattggggc attcatcata ggaatgggat tcgcctggtt cagcaggatg
    37561 acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca
    37621 accttctcaa cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg
    37681 gcattactga acgttttcgt caaccttctg gggtcttttg ccatgaccgc actggcattc
    37741 tggctgtttt cggcctcaac cgtacactaa agaccaaaaa aaacccgctg attaagcggg
    37801 ttttgaattc ttgctgacgt atcttacaga gcgattacgt ttgcagcaga agggcctttg
    37861 gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag caagagtttt aaaaccatta
    37921 gtctggattg cagagaagtg tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa
    37981 ccgaatcctt tggactcatt aaaccactta acgttacctt taatcttaga catcaaaatt
    38041 acctttacat gaaaaataga cacaaatgct gtgtcggtta tcagtacacc aattgtggta
    38101 cgctttgtcc agtgcaactt tactaaaaag tgataaaagt cgcgttattt ttgatgttgc
    38161 ttattttagt tattgatttt aaagaagtta ctaaaacttc atttgtctca aaactgaaag
    38221 cgcatccagg caaagtacac attgccattg ttgtaggtac ccggaatgta ggtcatctga
    38281 aaagtcactg ggccataacc cacggaggcc aatggcagta gaaccgggag agggatgtaa
    38341 ttccagttat cgcgtgccgt tacgccagcg gtgaatccca gacctaaatg aaaattttca
    38401 tccgccagcg gtcgccaggt actttcccat ccgtatccgg caatcggttc ccatttgttc
    38461 cacgagtcct taaatgccat ggcatacagg ccatgccagt ttcctttttc atcccaacgc
    38521 gacaggccaa aaccgccacc ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg
    38581 taagcgaaac gtgcatgcca ggtgatggca ggaatatata aatcataatg ttcaggctgt
    38641 tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc tctcatctgc gttagcaaaa
    38701 actttaccaa cgctgattaa ctgaataaaa acaaaggaaa agatagcgac atatttactc
    38761 acgttcattt gtgaccataa aacatttatc aaaaatctac tactagcata gcaaagctac
    38821 aattaacata accttaatag acccaacata aagaataatc tgaatagctg ctctatttat
    38881 ttccgcttgt tcaagttaaa tatttccgcg catcgattaa agatgatgcg tcgttttctg
    38941 cgatgggaat agtcaaaaaa gaaaaaccaa gtcttttttg atgacaaaat gcaatcaagg
    39001 aaaaattatt ttattttttt aacgagatac aacaatcatc ttaacgaagt atataatata
    39061 tatcgtcaac ccatgctcat attagattat tttttgagcg cacagccgca gctaacacca
    39121 ttgcaattaa caaatttgca tcaatccacc atcaatttac acacattatt atgtgataat
    39181 tgccaaccgc taaatatgcg ttttgttatc tatgtataaa aacagcaact tcaatgtctt
    39241 aatggcagtt tttcttgatt ttaatcagca ttcatcgcca atttattggg catatttttt
    39301 ccttaagctt taggaagttt ttattattta ctttgggcct ggagacagga aaaattatgc
    39361 tgacattcat tgaactcctt attggggttg tggttattgt gggtgtggct cgctacatca
    39421 ttaaagggta ttccgccact ggtgtgttat ttgtcggtgg tctgttattg ctgattatca
    39481 gtgccattat ggggcacaaa gtgttaccgt ccagccaggc ttcaacaggc tacagcgcca
    39541 cggatatcgt tgaatacgtt aaaatattac taatgagccg cggcggcgac ctcggcatga
    39601 tgattatgat gctgtgtggc tttgccgctt acatgaccca tatcggcgcg aatgatatgg
    39661 tggtcaagct ggcgtcaaaa ccattgcagt atattaactc cccttacctg ctgatgattg
    39721 ccgcctattt tgttgcttgt ctgatgtcac tggccgtctc ttccgcaacc ggtctgggtg
    39781 ttttgctgat ggcaaccctg tttccggtga tggtaaacgt tggtatcagt cgtggcgcag
    39841 cagctgccat ttgtgcctcc ccggcggcga ttattctcgc accgacttca ggggatgtgg
    39901 tgctggcggc gcaggcttcc gaaatgtcgc tgattgactt cgccttcaaa acgacgctgc
    39961 ctatctcaat tgctgcaatt atcggcatgg cgatcgccca cttcttctgg caacgttatc
    40021 tggataaaaa agagcacatc tctcatgaaa tgttagatgt cagtgaaatc accaccactg
    40081 cccccgcgtt ttatgccatt ttgccgttca cgccgatcat cggtgtactg atttttgacg
    40141 gtaaatgggg tccgcaatta cacatcatca ctattctggt gatttgtatg ctgattgcct
    40201 ccattctgga gttcctccgc agctttaata cccagaaagt tttctctggt ctggaagtgg
    40261 cttatcgcgg gatggcagat gcgtttgcta acgtggtgat gctgctggtt gccgctgggg
    40321 tattcgctca ggggcttagc accatcggct ttattcaaag tctgatttct atcgctacct
    40381 cgtttggttc ggcgagtatc atcctgatgc tggtattggt gattctgacc atgctggcgg
    40441 cagtcacgac cggttcaggc aatgcgccgt tttatgcgtt tgttgagatg atcccgaaac
    40501 tggcgcactc ttccggcatt aacccggcgt atttgactat cccgatgctg caggcgtcaa
    40561 acctgggtcg taccctatca cccgtttctg gcgtagtcgt tgcggttgcc gggatggcga
    40621 aaatctcacc atttgaagtc gtaaaacgca cctcggtgcc ggtgcttgtt gggctagtga
    40681 ttgttatcgt tgctacagag ctgatggtgc caggaacggc agcagcggtc acaggcaagt
    40741 aaatagtaat gccggaggca agtttcctcc ggctttatca tgtcacccac tgtggtatat
    40801 gcgttgtggt ctgccaactt tgccgtgaac aatttcagca ataatcagat ggcggctggc
    40861 gcaatattca agataacgcc tggcagtggt gcggctgatg gtcagtgcct gcgccaccgt
    40921 ttctgccgta tgttgcacac caggctcttt aaacagtttt cgcaccgcgt ttagcgtcaa
    40981 ggggtcaatg ccggtcggca gttcgtcttt gggttcaccg cgagcatagg cattaaacat
    41041 ctcatcaatt tgcttctggc tggcgctatc aatactttcc agcatatgtt tacgctggcg
    41101 gaaacgggtt agcgtttgcc ccagtgtatt gattcacttg aagtacgaaa aaaccgggag
    41161 aacattggat tattcgggat ctgatgggat tagatttggt ggggcttgca agcctgtagt
    41221 acaaatttta ggcgttaacc aatgaaacgc gaaagatagt aaaaaattgc ttttgtttca
    41281 ttgaaaaaac gaaaaacaaa aacactataa atcatttcaa tcatagcttc aaaaaacgtt
    41341 caaaaccgat aacaaccagg ctgtcaccaa atgactcata tcacaaatca gctcatgccg
    41401 tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg gctcatggtt
    41461 gttatacggc ttttttcgca tcttgtggtt ccaacaaagg agctacaggc tcttcctgtt
    41521 tatgaacacc atacattgac aaaatctctt ggcgagtttc tggaggtaat ttatgcagcg
    41581 ccaaagctgt ttgtagtgtt tggcgatcaa tacccagcgt tgccaatagg gtgtcaacgg
    41641 agatggaggg gcgttcttgc attaatccgg aaagcccata tttagccatg tttagctcta
    41701 tctctcggac ttcattttct cctagtttat tgattaacag ggagaacatt ttcaatgtat
    41761 cagcattcat ttcttcgata gcaatttttt gcatagccgg tattgccggg atatattcaa
    41821 ccccaggccc gcgacgccct tcttgtgaac gcttcggcca tccttctttc tttgctcgtt
    41881 tatgcacccc ctgtattgtc cctggcattc catccagttc agtaatttgt ttgggtgtca
    41941 tccactggtt tatctcattt gcgcttttca taaactaagt ttaccagatt tatctatata
    42001 aaaatcaatg gattacaaaa taaaccacat taactgatat aaaccagttt tgtttatttg
    42061 gtaatgtata aaacagataa tcaagacgaa ttatttgttt tggtaaccct ttatcatacg
    42121 caatatggtt ggtgtgcgaa agatgagtaa cgattggcat cgggccgacg tgattgcggc
    42181 cctcaaaaaa acaggaaccc ctctggcaaa attgtccaga cgttcaggtt attcaaccgg
    42241 gacgcttgca aatgcattag tcaggccatg gcctaaaggt gaacgcatta ttgctgacgc
    42301 cttaaacatg aaacctgagg aaatttggcc tagtagatat ctaagggatc acaatgagag
    42361 agtggatatc agcaattgaa tgcatgggtt tatctggttt acctggttca ttaatggggg
    42421 ttcacaaaag agctcagaga gaaggatggc tcaagagaag aagagaagga attagagggg
    42481 ttgcttacga ataccatata tcttctcttc ctaacattgc tcgagctgaa gttttgctcc
    42541 aacaaggaaa atttaaaacc agtcagggat gttttgaaat cgcccgcccc acgctggaag
    42601 ctcatgatta tgatcgtgag gcactatgga gcaaatggga taaagccagc gattcccagc
    42661 gcagcctggc tgaaaaatgg ttgccctcaa ttcaggccac agacgaaatg ctgaaccagg
    42721 ggatttcaac gaaaacggct tttgcgaccg ttgcagggca ttaccaggtc agcgcatcca
    42781 ctttgcggga caagtattac caggtacaga agtttgcgaa gcctgactgg gcggctgcac
    42841 ttgttgatgg acgtggagca tcccgtcgca atgttcacaa aagtgaattt gacgaggatg
    42901 cctggcagtt tctgattgca gattatctgc gaccggaaaa acccgccttc cgcaaatgtt
    42961 atgagcgtct ggaactggca gtccgcgagc atggctggag tattccctcc cgtgccacgg
    43021 cctttcgccg gattcagcaa ctggacgagg caatggttgt tgcctgtcgt gaaggtgaac
    43081 atgcactgat gcatctgata ccggcacagc agcgaactgt ggaacacctg gacgccatgc
    43141 agtggatcaa cggcgacggt tatctgcata acgtctttgt acgctggttt aacggtgatg
    43201 tgatccgtcc gaaaacatgg ttctggcagg atgtgaaaac ccgaaaaatt ctgggctggc
    43261 gctgcgatgt gagcgagaac attgattcaa ttcgcctctc gttcatggat gttgtgactc
    43321 gctacagtat cccggaggat tttcacatca ccattgataa cacccgtggt gctgcgaata
    43381 aatggctgac gggaggcgcg cccaatcgct accgctttaa
//
LOCUS       DAJVPP010000024        43019 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVPP010000024 DAJVPP010000000
VERSION     DAJVPP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.024"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1279
                     /gene="cscA"
                     /locus_tag="OQM34_001290"
     CDS             <1..1279
                     /gene="cscA"
                     /locus_tag="OQM34_001290"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCS1451813.1"
                     /translation="FYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDG
                     CFSGSAVDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPE
                     GIMHFRDPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESY
                     MWECPDFFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTEL
                     DNGHDFYAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQ
                     RPVHEAESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGM
                     RLYIDNQSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVM
                     SSRIYPQPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1287..2345)
                     /locus_tag="OQM34_001291"
     CDS             complement(1287..2345)
                     /locus_tag="OQM34_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1451814.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2513..2731
                     /locus_tag="OQM34_001292"
                     /pseudo
     CDS             2513..2731
                     /locus_tag="OQM34_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2749..4077
                     /gene="dsdA"
                     /locus_tag="OQM34_001293"
                     /pseudo
     CDS             2749..4077
                     /gene="dsdA"
                     /locus_tag="OQM34_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(4185..5723)
                     /gene="emrY"
                     /locus_tag="OQM34_001294"
     CDS             complement(4185..5723)
                     /gene="emrY"
                     /locus_tag="OQM34_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1451815.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5723..6886)
                     /gene="emrK"
                     /locus_tag="OQM34_001295"
     CDS             complement(5723..6886)
                     /gene="emrK"
                     /locus_tag="OQM34_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1451816.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7302..7916
                     /gene="evgA"
                     /locus_tag="OQM34_001296"
     CDS             7302..7916
                     /gene="evgA"
                     /locus_tag="OQM34_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1451817.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            7921..11514
                     /gene="evgS"
                     /locus_tag="OQM34_001297"
     CDS             7921..11514
                     /gene="evgS"
                     /locus_tag="OQM34_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1451818.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11570..12715)
                     /gene="yfdE"
                     /locus_tag="OQM34_001298"
     CDS             complement(11570..12715)
                     /gene="yfdE"
                     /locus_tag="OQM34_001298"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1451819.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12789..13733)
                     /gene="yfdV"
                     /locus_tag="OQM34_001299"
     CDS             complement(12789..13733)
                     /gene="yfdV"
                     /locus_tag="OQM34_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1451820.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13803..15497)
                     /gene="oxc"
                     /locus_tag="OQM34_001300"
     CDS             complement(13803..15497)
                     /gene="oxc"
                     /locus_tag="OQM34_001300"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1451821.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15551..16801)
                     /gene="frc"
                     /locus_tag="OQM34_001301"
     CDS             complement(15551..16801)
                     /gene="frc"
                     /locus_tag="OQM34_001301"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1451822.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17314..17937)
                     /locus_tag="OQM34_001302"
     CDS             complement(17314..17937)
                     /locus_tag="OQM34_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1451823.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18234..18509
                     /locus_tag="OQM34_001303"
     CDS             18234..18509
                     /locus_tag="OQM34_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451824.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18586..18828)
                     /locus_tag="OQM34_001304"
     CDS             complement(18586..18828)
                     /locus_tag="OQM34_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1451825.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19181..20101
                     /gene="lpxP"
                     /locus_tag="OQM34_001305"
     CDS             19181..20101
                     /gene="lpxP"
                     /locus_tag="OQM34_001305"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1451826.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20455..20526
                     /gene="ypdK"
                     /locus_tag="OQM34_001306"
     CDS             20455..20526
                     /gene="ypdK"
                     /locus_tag="OQM34_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1451827.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20591..21829)
                     /gene="alaC"
                     /locus_tag="OQM34_001307"
     CDS             complement(20591..21829)
                     /gene="alaC"
                     /locus_tag="OQM34_001307"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1451828.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22206..23692
                     /locus_tag="OQM34_001308"
                     /pseudo
     CDS             22206..23692
                     /locus_tag="OQM34_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            23707..24441
                     /gene="ypdB"
                     /locus_tag="OQM34_001309"
     CDS             23707..24441
                     /gene="ypdB"
                     /locus_tag="OQM34_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS1451829.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24454..25311
                     /locus_tag="OQM34_001310"
     CDS             24454..25311
                     /locus_tag="OQM34_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1451830.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25314..27809)
                     /gene="ptsP"
                     /locus_tag="OQM34_001311"
     CDS             complement(25314..27809)
                     /gene="ptsP"
                     /locus_tag="OQM34_001311"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1451831.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(27834..28871)
                     /gene="ypdE"
                     /locus_tag="OQM34_001312"
     CDS             complement(27834..28871)
                     /gene="ypdE"
                     /locus_tag="OQM34_001312"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1451832.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(28871..29954)
                     /gene="ypdF"
                     /locus_tag="OQM34_001313"
                     /pseudo
     CDS             complement(28871..29954)
                     /gene="ypdF"
                     /locus_tag="OQM34_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(29969..31216)
                     /locus_tag="OQM34_001314"
     CDS             complement(29969..31216)
                     /locus_tag="OQM34_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1451833.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31238..31564)
                     /locus_tag="OQM34_001315"
     CDS             complement(31238..31564)
                     /locus_tag="OQM34_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1451834.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31783..32748)
                     /gene="glk"
                     /locus_tag="OQM34_001316"
     CDS             complement(31783..32748)
                     /gene="glk"
                     /locus_tag="OQM34_001316"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1451835.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMVIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            32952..34208
                     /locus_tag="OQM34_001317"
     CDS             32952..34208
                     /locus_tag="OQM34_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1451836.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34323..34649
                     /locus_tag="OQM34_001318"
     CDS             34323..34649
                     /locus_tag="OQM34_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1451837.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34790..36028)
                     /locus_tag="OQM34_001319"
     CDS             complement(34790..36028)
                     /locus_tag="OQM34_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1451838.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36364..37566
                     /gene="nupC"
                     /locus_tag="OQM34_001320"
     CDS             36364..37566
                     /gene="nupC"
                     /locus_tag="OQM34_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1451839.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37616..38931)
                     /locus_tag="OQM34_001321"
                     /pseudo
     CDS             complement(37616..38931)
                     /locus_tag="OQM34_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(39071..39697)
                     /locus_tag="OQM34_001322"
     CDS             complement(39071..39697)
                     /locus_tag="OQM34_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCS1451840.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRIYLKLAGSVKRAATSVVHTL"
     gene            complement(39905..39980)
                     /locus_tag="OQM34_001323"
     tRNA            complement(39905..39980)
                     /locus_tag="OQM34_001323"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39945..39947),aa:Ala,seq:ggc)
     gene            complement(40020..40095)
                     /locus_tag="OQM34_001324"
     tRNA            complement(40020..40095)
                     /locus_tag="OQM34_001324"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40060..40062),aa:Ala,seq:ggc)
     gene            40316..40675
                     /locus_tag="OQM34_001325"
     CDS             40316..40675
                     /locus_tag="OQM34_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1451841.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40677..41069
                     /locus_tag="OQM34_001326"
     CDS             40677..41069
                     /locus_tag="OQM34_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1451842.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41121..42536)
                     /gene="gltX"
                     /locus_tag="OQM34_001327"
     CDS             complement(41121..42536)
                     /gene="gltX"
                     /locus_tag="OQM34_001327"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1451843.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGAFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            42795..42870
                     /locus_tag="OQM34_001328"
     tRNA            42795..42870
                     /locus_tag="OQM34_001328"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42828..42830,aa:Val,seq:tac)
     gene            42915..42990
                     /locus_tag="OQM34_001329"
     tRNA            42915..42990
                     /locus_tag="OQM34_001329"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42948..42950,aa:Val,seq:tac)
BASE COUNT        11427 a        10723 c        10012 g        10857 t
ORIGIN      
        1 gttttatcaa catcacccga tgagcgaaca ctggggacca atgcactggg gacatgccac
       61 cagtgacgat atgatccact ggcagcatga gcctattgcg ctagcgccag gagacgataa
      121 tgacaaagac gggtgttttt caggtagtgc tgtcgatgac aatggtgtcc tctcacttat
      181 ctacaccgga cacgtctggc tcgatggtgc aggtaatgac gatgcaattc gcgaagtaca
      241 atgtctggct accagtcggg atggtattca tttcgagaaa cagggtgtga tcctcactcc
      301 accagaagga atcatgcact tccgcgatcc taaagtgtgg cgtgaagccg acacatggtg
      361 gatggtagtc ggggcgaaag atccaggcaa cacggggcag atcctgcttt atcgcggcag
      421 ttcattgcgt gaatgggcct tcgatcgcgt actggcccac gctgatgcgg gtgaaagcta
      481 tatgtgggaa tgtccggact ttttcagcct tggcgatcag cattatctga tgttttcccc
      541 gcagggaatg aatgccgagg gatacagtta ccgaaatcgc tttcaaagtg gcgtaatacc
      601 cggaatgtgg tcgccaggac gactttttgc acaatccggg cattttactg aacttgataa
      661 cgggcatgac ttttatgcac cacaaagctt tttagcgaag gatggtcggc gtattgttat
      721 cggctggatg gatatgtggg aatcgccaat gccctcaaaa cgtgaaggat gggcaggctg
      781 catgacgctg gcgcgcgagc tatcagagag caatggcaaa cttctacaac gcccggttca
      841 cgaagctgag tcgttacgcc agcagcatca atctgtctct ccccgcagaa tcagcaataa
      901 atatgttttg caggaaaacg cgcaagcagt tgagattcag ttgcagtggg cgctgaagaa
      961 cagtgatgcc gaacattacg gattacagct cggcactgga atgcggctgt atattgataa
     1021 ccaatctgag cgacttattt tgtggcggta ttacccacac gagaatttag acggctaccg
     1081 tagtattccc ctcccgcagc gtgacacgct cgccctaagg atatttatcg atacatcatc
     1141 cgtggaagta tttattaacg acggggaagc ggtgatgagt agtcgaatct atccgcagcc
     1201 agaagaacgg gaactgtcgc tttatgcctc ccacggagtg gctgtgctgc aacatggagc
     1261 actctggcta ctgggttaac ataatatcag gtggaacaac ggatcaacag cgggcaaggg
     1321 atccgcgtca ctcttccccc ttcacgacct tcaataatat gcaatgcagc ttcccgcccg
     1381 ataatgtcat gtggaagctg aattgtggtc agcggcggta aaaacagatg cccgacgcca
     1441 accagattat caaagcccat tacagcgaca tcctgcggga tacgtacccc cttcgccaaa
     1501 agaacctgat aagccacaaa ggctgcgcga tcgttaccac atatcagaac atcaaaatct
     1561 ggtttgcccg atttgaagtg agcattgagt aaacttgcga gatcggtgta gtgatcatca
     1621 cctgttgcca tgtgaaattg tttcacctca gccagatctc gtccagcatc acgccaagcc
     1681 tgctcaaatc cctgccgacg ataccctgtt gccaacgcac tttccggtag ccagaagcat
     1741 aatggttgac gatagcccgc cgcgagcaaa tgctgtgttg attcatattg tgcagtgtaa
     1801 tcatcaggga tataactggg taacgctggg tcatccgcca cacagttcgc caatacaata
     1861 ttttcaccat acagagactc aggcagcgtg atatgtcgca gccccattgt agtatagata
     1921 atgccatccg gacggtgggc aagcagctga cgtgccgcgc gggcagcgtc atcttcagaa
     1981 aaaatattga ttaaaaaact attccagccg aactcgctgg cggtttgctc aatggcaagc
     2041 agaatatcaa cagagaaagg agtggtagcc gtgtcctgcg ccagcacggc gagagtcgac
     2101 ggcttacgtc cttgagcgcg catcttacgg gcggaaagat caggaacata attcagggtc
     2161 tggattgcct gcaatacgcg gtcacgcgtt gcaggacgca cagattctgc attatgcatc
     2221 acccgggaga ctgtcatcat cgacactccc gccaggcgtg cgacatcctt taatgaagcc
     2281 atacccaagc cgtttgccgt aaaacgggca ctgtagcaga aacagacgtc actggcgaga
     2341 tccaacgccc tatcacctga cacagcaata caataaaaaa taacaataat tcccggacaa
     2401 ttgtccccaa ttccgcctct gttctcgcca acgagtaacc atataggcaa gtaagtaaaa
     2461 catgccaact ggcatcgcca acctggagaa gaaaatataa atatggatca ttgtactgcc
     2521 actgtatccc gacatcagcc cggaaattat tgcgattgct atcggttcag gtgcaattgg
     2581 ctgcacgatc gttacggact cgcttttctg gctggtgaag caatattgcg gcgctacgct
     2641 caatgaaaca tttaaatact atacgacagc gacatttatc gcttcagtcg tcgctctggc
     2701 gggcacattc ctgctgtcat ttatcatcta agcgcaaaga gacgtactat ggaaaacgct
     2761 aaaatgaact cgctcatcgc ccagtatccg ttggtaaagg atctggttgc tcttaaagaa
     2821 accacctagt ttaatcctag cacgacctca ttggctgaag gtttacctta tgttggcctg
     2881 accgaacagg atgttcagga cgcccatgcg cgcttatccc gttttgcacc ctatctggca
     2941 aaagcatttg ctgaaacagc tgccactggg gggattattg aatcagaact ggttgccatt
     3001 ccggctatgc aaaaacggct ggaaaaggaa tatcaacaac cgatcagcgg gcaactccta
     3061 ctgaaaaaag atagccattt gcccatttcc ggctctataa aagcacgcgg cgggatttat
     3121 gaagtcctgg cacatgcaga aaaactggct ctggaagcgg ggttgctgac gcttgatgat
     3181 gactacagca aactgctttc tccggagttt aaacagttct ttagccaata cagcattgct
     3241 gtgggctcaa ccggaaatct ggggttatca atcggcatta tgagcgcccg cattggcttt
     3301 aaggtgacag ttcatatgtc tgctgatgcc cgggcatgga aaaaagcgaa actgcgcagc
     3361 catggcgtta cggtcgtgga atatgagcaa gattatggtg ttgccgtcga ggaaggacgt
     3421 aaagcagcgc agtctgaccc gaactgtttc tttattgatg acgaaaattc ccgcacgttg
     3481 ttccttgggt attccgttgc tggccagcgt cttaaagcgc aatttgccca gcaaggccgt
     3541 atcgtcgatg ctgataaccc tctgtttgtc tatctgccgt gtggtgttgg cggtggtcct
     3601 ggtggcgtcg cattcgggct taaactggcg tttggcgatc atgttcactg cttttttgcc
     3661 gaaccaacgc actccccttg tatgttgtta ggcgtccata caggattaca cgatcagatt
     3721 tctgttcagg atattggtat cgacaacctt accgcagcgg atggccttgc agttggtcgc
     3781 gcatcatgct ttgtcgggcg ggcaatggag cgtctgctgg atggcttcta tacccttagc
     3841 gatcaaacca tgtatgacat gcttggctgg ctggcgcagg aagaaggtat tcgtcttgaa
     3901 ccttcagcac tggcgggtat ggccggacct cagcgcgtgt gtgcatcagt aagttaccaa
     3961 cagatgcacg gtttcagcgc agaacaattg cgtaatgcca ctcatctggt gtgggcgacg
     4021 ggaggtggaa tggtgccgga agaagagatg aatcaatatc tggcaaaagg ccgttaataa
     4081 cgtttcaacg caacatcgca atcctttccc tgggtgagcg atgctgccga tggcgcagac
     4141 ttaagatccc cggtcttacc cgctataatc ccctcctttt atcatcaccc aacgcctttc
     4201 gctgtaaacg gcggtttcgc aaaccaaacc aaaaccgtaa gtaagataaa agcaatagcc
     4261 gccatacgga aaatctcatt tgcagaaata gaaagtgact gctgggttat ttcattatta
     4321 atttcattaa gaactcctga aagcgaacca tagtatttat ccataatttg tgacgatgaa
     4381 ttaaacacgg ggttaaattg atcgatggtt gctgtcaact gactatggtg taacgattcg
     4441 cgtcgtcccc acagcgtcat tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa
     4501 aaattactca tactcgaggc attggcaaat ttattatctg gcaagcctga aaacgaaatc
     4561 gttgttaagg gtaaaaagaa acaggcaacg gcgaatccct ggaaaaactg tggcaaaatg
     4621 atgcctgtaa aatcaatcgt tggcataaat gtcacagaac gccagtaata gcaaaccgca
     4681 tacatcaaaa aactgaatgt cactaacacc cgcatgtcta ttttattgcc ataacgtcct
     4741 atcaaaggtg aaatcaatag tggcatgatg ccgatgggcg cataagcaag cccggcccat
     4801 atcgcattat accccatcgt tttctggagt aactgcggca taaggacgat cgctccagag
     4861 taaaataaat acgcgcaggt gatactcaca ataccaatgg tgaaattacg ggacttaaac
     4921 aaactgagat caagaatcgg gttctctgag gtcgattccc aaatgactaa agagatcaga
     4981 gaaataactg atactactgt taatattatt attgtactcg agttgaacca atccagatcg
     5041 cgccctttat caagcataat ttgcaagcca ccaacaccga gcactaacag ggtcagtctt
     5101 ggtagattca tttttaccgg tgaagtttca gtttctcttc ctttaagtaa ggttaagcat
     5161 aatgtcagga cgataatccc catagggaca ttgattaaaa atatccaacc ccagctaaag
     5221 ttatcgcaaa tatagccgcc caatatcggc ccacatatcg gagcgataat cacggtcatt
     5281 gaccataatg ccagagcaaa tgttcttttt tctggtggat aattccttaa taataaactc
     5341 tgtgacagtg gaattaacgg ccccgccatt aatccctgaa cgactctaaa aaatatcagc
     5401 acatcaagat tggtcgataa actacacatt aaggaagaca gcgaaaaaaa agtgactgaa
     5461 agtaaaaata accttaattc gcctattctt tgtgccagcc tgccggtaac agggatcgca
     5521 atggcatttg ctacgccaaa cgaggtgata acccaggtgc cttcgtctgt tgatgctccc
     5581 agaaagccag atattgtcgg tattgcgacg ttagaaatag tggaatccaa catttgcata
     5641 aacgtcgcta atgacaatgc aatagtaacg caccataacg tcccaccggt taatggtgcc
     5701 ggagttgatt tagtgattgc cattaaagtt gtccattatg cgaaataatg ttgctaattt
     5761 ctttttctat cggactggta tcgataacta aagccttact ggtataagca ggcatggagg
     5821 tcacggttga agccagatca ggcatctcag caatgtcttc gtccttcgta tcaatagttg
     5881 cagtcatcga taaaccaatg cgcaaggggt gttccatgag ttcttttgga tcaagagaaa
     5941 cttcaaccgg tacacgctga acgattttga tccagttccc tgtcgcattt tgtgcaggta
     6001 ataaggagaa cgcattgccg gttcccatat tgatccctgt cacccgacca tgaaacacaa
     6061 cattttcacc ataaagatcg ctgataatat tgactgattg accaatccgt acatccgtga
     6121 gttgtgtttc tttaaagttg gcattaaccc acatttgacg tgccggtacg acagccatta
     6181 acgattgtcc ggaactcact gtttcgccga cctgaacact tcgctgggca atatagccag
     6241 taaccggact ccttatatcc gtacgtttaa gcaccaacca ggcttctttg gttgcatccg
     6301 ccgcttcaac tacttgtggc tgacggttta atggtgtgtt cattactaaa gctttattcg
     6361 ctttataagc ctggatagcg gcattcagtg ccgctttgct acttattaac gtatctttgg
     6421 tatgctccag cgtttctttt gaaataaccc cctgcttcgc taaaggcaca cgacggttat
     6481 aatcttctaa agattgttga tactgaatac gtgctgaagc gacttcggca ctgtattgtt
     6541 tatcctgtaa gtacagttta ttcgtttgcc gaacaatatt tgccagatta tttttagctt
     6601 tattgagtgc gatagtggca tcagttttat ccagtgaaac taaaatgtca ccctgtcgaa
     6661 cgtagttcgt atctttatga ttaactacag tgacactacc tgagacttgt gcagaaattg
     6721 gatctgcatt ccccgtgaca taggcgtcat ctgtactaat catgtcttct aattccattg
     6781 accaataggc ataggcacct gaaaacgcaa taaataaaac tatcaccaat aaagaaaagt
     6841 atttttttct gttagactgt tttttatttg aattaatctg ttccactatt atctctcatt
     6901 tcgcatagat gaaatttatg aattgaatga aaagagtatt cattcttcat atcaaacaca
     6961 gtgcatgtgt tgcacattat cccaggaacg ttggaggcct aatttaaatg aggtgcaaaa
     7021 aaaacaacag aggactaaac cgtggctttt gcaatacaat tcttacgcct gtaggattag
     7081 tgagaagact tatagtgcta acttgaaact ataaatcatc ggtataatcc ctgattttat
     7141 tgttgacatt tcatttatgc cgactattta tatggtatac ttgtcgaatt atcttaaagg
     7201 aaactcagat tttcttattt ttattgagaa aatgagatga cgccttatgt ctgtattact
     7261 acagggagaa gggagatgct tcattgcaaa gggaataatc tatgaacgca ataattattg
     7321 atgaccatcc tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa
     7381 tcttagcgga gttaactgaa ggcggaagcg ccgttcagcg ggtggaaaca cttaagcctg
     7441 atatcgtcat cattgatgtc gatatccccg gagttaacgg tatccaggtg ttagaaacgc
     7501 tgaggaagcg ccaatatagc ggaattatta ttatcgtctc cgctaaaaat gaccattttt
     7561 acgggaaaca ttgtgctgat gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga
     7621 acaatatcat tgcggctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc
     7681 tcaaccggtt tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac
     7741 aagaaattag tgtcatgcgg tatattcttg atggcaagga taataatgac attgcggaaa
     7801 aaatgttcat cagcaacaaa actgtcagca cgtataaaag tcgcctgatg gaaaaattag
     7861 aatgtaaatc actgatggat ctttacacat tcgcgcaacg taacaaaatc ggctaatcac
     7921 atgaagtttt taccctattt ttttcttctc tgttgtggtc tttggtcgac cataagtttc
     7981 gcagacgaag attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat
     8041 ccagtacgtc tgagcaacaa ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt
     8101 gcagtacaca agtcccaaac ggctaccttg ttgcataccg attcgcagca acaggttcgt
     8161 ggtattaatg ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc
     8221 cgggaatacg cagatcatca aaaagcaatg gacgcgcttg cagaaggtga agtcgatata
     8281 gtgttatcac atttagttac ttcgccgcct cttaataatg acattgctgc aaccaaacca
     8341 ttgataatta cctttccggc gctggtaacc acccttcacg actcaatgcg accgcttacc
     8401 tcaccaaaac cagtaaatat tgctcgggta gcaaattacc ccccagacga ggtaattcat
     8461 caatcatttc caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc
     8521 gtctcagctg ggcacaatga ttactttatt ggtagtaaca tcattaccag cagtatgatt
     8581 tcccgctatt tcactcactc cttaaatgta gtgaaatatt ataactcgcc gcgtcaatat
     8641 aattttttct tgaccagaaa agaatctgtc attcttaatg aagtactcaa tagatttgtt
     8701 gatgctttaa caaatgaagt tcgctatgaa gtatcacaaa attggcttga tacaggaaac
     8761 ctggcctttc tgaacaaacc attagaactc actgaacatg aaaaacagtg gattaagcag
     8821 catcccaatt taaaggtgct ggaaaatcct tactcgcccc cctattctat gacggatgaa
     8881 aatggctcgg ttcggggcgt tatgggggac attcttaata ttattacctt gcaaacaggt
     8941 ttaaattttt ctccgatcac cgtttcacac aatatccatg ctggaacaca acttagcccc
     9001 ggaggatggg atataatacc tggcgctatt tatagtgaag atcgagaaaa taatgtttta
     9061 tttgctgaag ccttcataac aacgccttac gtttttgtca tgcaaaaagc gcctgacagt
     9121 gaacaaacat taaaaaaagg aatgaaagtt gccattccat attattatga gctgcattcg
     9181 caattaaaag agatgtatcc ggaggttgaa tggatacagg tcgataatgc cagcgctgca
     9241 tttcacaagg ttaaggaagg tgaacttgat gctctggtcg cgacacagct aaattcgcgt
     9301 tacatgatcg atcattacta tcctaatgaa ctttatcatt ttcttattcc tggcgttccg
     9361 aatgcatcgc tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga tattattaat
     9421 aaagcactga atgcaattcc cccaagcgaa gttctgcgcc tgacggaaaa atggattaaa
     9481 atgcccaatg tgaccattga cacatgggac ctatatagcg agcaatttta tattgttacg
     9541 acattatccg ttttattagt tggcagtagc cttttatggg gattctacct gttacgctca
     9601 gttcgtcgtc gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt ccgaaaagca
     9661 ctctcggatt ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa tgtcattagt
     9721 cataatagtg cttttgaaca ttatttcact gcggattact acaaaaatgc aatgttacca
     9781 ttagaaaaca gtgattcacc ctttaaagat gttttttcta atgcgcatga agtcacagca
     9841 gaaacgaaag aaaatcgaac aatatacaca caggtatttg aaattgataa tggcatcgag
     9901 aaaagatgca ttaatcactg gcatacatta tgcaatcttc ctgcaagtga caatgcagta
     9961 tatatttgtg gttggcaaga tattactgaa acgcgtgatc taattaatgc actcgaggta
    10021 gaaaaaaata aagcgataaa ggctaccgta gcaaaaagtc agtttctggc aacgatgagt
    10081 cacgaaataa gaacaccaat aagctctatt atgggcttcc tggaacttct gtcgggttct
    10141 ggtcttagca aggagcaacg ggtggaggcg atttcacttg cctacgccac cggacaatca
    10201 ctcctcggct taattggtga aatccttgat gtcgacaaaa ttgaatcggg taactatcaa
    10261 cttcaaccac aatgggtcga tatccctact ttagtccaga acacttgtca ctctttcggt
    10321 gcgattgctg caagcaaatc gatcgcatta agttgcagca gtacatttcc tgatcattac
    10381 ctggttaaga tcgaccctca ggcgtttaag caggtcttat caaatttact gagtaatgct
    10441 ctcaaattta ccaccgaggg ggcagtaaaa attacgacct ccctgggtca cattgatgac
    10501 aaccacgctg ttatcaaaat gacgattatg gattctggaa gtggattatc gcaggaagaa
    10561 caacaacaac tgtttaagcg ctacagccaa acaagtgcag gtcgtcagca aacaggttct
    10621 ggtttaggct taatgatctg caaagaatta attaaaaata tgcagggcga tttatcatta
    10681 gaaagtcatc caggcatagg aacaacattt acgatcacaa tcccggtaga aattagccag
    10741 caagtggcga ctgtcgaggc aaaagcagaa caacccatca cactacctga aaagttgagc
    10801 atattaatcg cggatgatca tccgaccaac aggctattac tcaaacgcca gctaaatcta
    10861 ttaggatatg atgttgatga agccactgat ggtgtgcaag cgctacacaa agtcagtatg
    10921 caacattatg atctgcttat tactgacgtt aatatgccga atatggatgg ttttgagttg
    10981 actcgcaaac tccgtgagca aaattcttcc ttacccatct gggggcttac agccaacgca
    11041 caggctaacg aacgtgaaaa agggttaagt tgcggcatga acttatgttt gttcaaaccg
    11101 ttgaccctgg atgtactgaa aacacattta agtcagttac accaggttgc gcatattgca
    11161 cctcagtatc gccaccttga tatcgaagcc ctgaaaaata atacggcgaa tgatctacaa
    11221 ctgatgcagg agattctcat gactttccag catgaaacgc ataaagatct acccgctgcg
    11281 tttcatgcac tagaagctgg cgataacaga actttccatc agtgtattca tcgcatccac
    11341 ggtgcggcta acatcctgaa tttgcaaaag ttgattaata ttagccatca gttagaaata
    11401 acacctgctt cagatgacag taagcctgaa attcttcagc tgctgaactc tgttaaagaa
    11461 cacattgcag agctggacca ggagattgct gttttctgtc agaaaaatga ctaaattgcg
    11521 gctcccacaa tgttctaatg tgggagctat ttaccagcac atcttacatt tatgatgaga
    11581 actcctggcg aatttgttcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg
    11641 ggtccgcgca gccgctgatt tttatcggat tacccggcat cattattcct ccggcttcaa
    11701 tcaacatatt tctcgcctga gtttgtggca aattaatggc ctcagccaca cttaataacg
    11761 gcgcgacggg cacaccaact tcatgtattt tggctaacca aacttcagct gcctgcgttt
    11821 ttaacgttcg ctcaatatat tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc
    11881 tgctaaatcg gggatcatta accagttccg taagctccag tgcctggcat aacgcagaaa
    11941 aaagcttgtc attaccacaa caaatcgtaa tcggcttatc ctgagtatcg aaaacatcaa
    12001 aaggtgccat gtagggatgg cgatttccca ggcgttgtgg tgacttccct gtcgcaatat
    12061 atgccatcag accatgctcc agaaaactca gcgtggcatc aaacatcgct atatcgacat
    12121 gcgcccctct ctggctcttt tcgcggccat aaagtgcact cactattccg ctgaataaat
    12181 agacaccgcc gcatagatcc gcaagagagg taccaacacg cactggcgga gcatcagggt
    12241 accctgtttc catcattatc ccactcattg cctgaatgat ggtatcgtag gcaggagcat
    12301 cttttagcgg accggtatgt ccgaaacctg acgatgaagc atatatgagg cgcgggttga
    12361 tttcttgtaa cctttcccat gaaaacccca gtttttccat tgtacctagg cgaaaattct
    12421 cagctaatac atcagcttgt ttgagcatat ttataaatat acttttatcg tgatcattct
    12481 ttaaatcaag aaccacactc tctttgccat gattaataaa actgtaatag agtgactgtc
    12541 catccacata gggaccaaat gtgcgggtat catcaccatg acctggtggc tcaactttaa
    12601 ttacccttgc gcccatatta taaagaagtt gagttccgaa aggtccatta aggacatgtg
    12661 tcatatcgat aactaataag ccttcaaacg gccctttgct ttcattattt gtcatttgct
    12721 tatgccttga aaaaagccga aggtgaaaag acacttcggc atttatgtaa aatcaataac
    12781 atgattaatt atgaaaccag tcgactgaca taaatccaca agggagccgt gacgacaaag
    12841 cccagtacgc tcacagccaa tgacgctgta ccagtacggg tgtagacatt aaaccggctg
    12901 gcaataatga ttccggagaa tgccggcggt aatgcgcctg ccagtaccat catctgcaaa
    12961 tgttcgctgt tcaaatgaca tgccatacca acgagaagca gcgccagtgg catcagaatc
    13021 aacttcagga aggtgttata agcaatttcg gcactgaact cgaatttatg tgcagccaga
    13081 gtcaaccccg cagcgaatac cgctacccct gagttagcct tcgcaatcag attaaaggtt
    13141 gggtcccatg ctgccggaat ttttacccca accagcacca ggatcgttgc cagaacaggt
    13201 gcccatacta ctggctcctt tgccgcagaa attaatgcgc tcagattact attcttctta
    13261 ccatccgctc ctgaggaagg attcagcaaa tacagaccaa taggaatagt aattgcgtta
    13321 acaataatag aaataattgc taccactaaa cctgttgata cggaatcacc ataaatagga
    13381 tcgagaactg caaaccccag gaatccaatg gtaggtgaac ccgcaattaa tgcacataca
    13441 gctgcttctg catgggtacg tttaaaaaat ttgtagcaac caaaccagga gaaaaagaaa
    13501 catccgacaa taaccacaag tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc
    13561 ctgtttgccc gggtaataga aacaaataga gccgcaggaa gagcgtagtt taataccagt
    13621 ttattaaatg cgcgggcttg atcttctgaa aaagtttctc gtctgccgct aaaataaccc
    13681 aacagcatga ttacgataat aggcaataaa tcgccaataa aaaatgttag cataatagta
    13741 tttccgataa gggtcccctc accatcgcat aatgagttgg caagaggaga cgatgtcagt
    13801 atttaattac cagcgacttg ttttgggtta agtttggtaa tatggccact ttcagttcct
    13861 gctgcagggt cgataaccac attaataatg gtcggtttgc gcgactgaat accggtggtt
    13921 aaagcatgac gaagttcatc tgtcgtggtg acgttatagc caacgccacg aaacgcatcc
    13981 attaatttgt cataccttgc atggtgcaac agatccgttg gtgatggtgc accagcgcca
    14041 ctgagatcaa caccgtctcc tctgtagatg ccgccattat taaaaataac gatcgtcacc
    14101 ggcaggttat atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa agcactatca
    14161 ccttcaatgg cgacaaccgg agaaccagaa gtcacgctag caccgatggc atagcccata
    14221 ccgatgccca tgacacccca ggtaccacaa tccagacgac gacgtggttt atacatatca
    14281 ataatatttc gtgcattatc cagggtattt gcaccttcat taactaaata aatatcctgg
    14341 ttctcgcgca atacgtcgcg cacagcactt aatgcattaa agtaatttaa tggttgagta
    14401 tctgtactta atttttcatg cattttttgt gcattttgct gcttgtggat atttaaaata
    14461 tcgcgccata ccagtggagt cgtaaatgtg ttttgtttca gttctgccag cataccttgc
    14521 atactggatg caatatcacc aacgactggc acagcaatgg ggcggttgct gtcaatttcc
    14581 tgcggttcaa tatccagttg aataaactgt gtatctgccg cccatccttt tttaccgtgt
    14641 gccaataacc aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt tgccagggca
    14701 aacgaacgcg cagctgccgc agaaagtgga tgcgtatctt caaggatccc tttcgccata
    14761 gacattggca ggaatggaat ctgagcactt tcaataaatt cacgaagctg ttcatcagct
    14821 tgtgaatacg ccgcgccttt gccaaggata attaatggcc gttcagcttt tgctaaaagc
    14881 gaaattgcgc tagtgactga cttcgggcat ggcaataatg ctggcgacgg attttcaact
    14941 ttaacaatcg tggttaacgc ttcgtctttt tccatcgtcg cggccaggac atttgctggc
    15001 aaatcaagat aaactccgcc agggcgaccc gatacagaga cccggatagc gcgtgccaat
    15061 gcaatgccaa gatcctgcgg ctgattaacg cgaaatgctg ctttggcata cggttttgcc
    15121 gcattcattt ggtccagctc ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg
    15181 ctggagccgc taatcatgat aatcggaaaa ccatttaccg ttgcgttggc caatgcggtc
    15241 aaaccattga ggaatcctgg cgcagaaact gtcaggcaga tccccggttt ttgggtaaga
    15301 aaaccgcttg ccgcagcggc atagcctgcc gactgctcat gacgaaaacc aatataacga
    15361 atgccttccg cctgggcatg gcgtgccata tccgtcacag gaatacctac aacaccataa
    15421 atagtgtcaa tattattctg ttttaatgct tcaacgatga tatgcatacc atctgtcatt
    15481 tgaagttgat ctgacatgat taataacctc gaaattttta taaattggga gccagaagcc
    15541 cccgttaata tcagatggcg tggttttgct tcattgcagc aatttcatcg tcgctataac
    15601 ccagctcctg caatacagca gcggtatgtt cacctaatag cggcgcagct ttaatatccg
    15661 gcgtaaaggc agagaatttc attggacaac caacggtcag atattttcca cgcaacggtt
    15721 gttccacttc aacaacactg ccactttggc gcaaagaggg atcaagtgaa atttctttca
    15781 tacttaaaac cggtgcacaa ggaatatcaa actgagtcaa ataggccacc gcttcatgtt
    15841 tatcaatagt gacagtgtat ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc
    15901 gggcatgggc tgtactgtat gccggatcgg taatccattc tggtttgccg atggctttac
    15961 aggtgttttc ccagttttgc tcctgaatag tgaaataaat ataggcgtta ggatcggttt
    16021 cccagccttt acatttcagg atccagccag gctgaccgcc accacctgca ttaccaccgc
    16081 ggggaactgc atcaccaaat gtaccattcg gatactgcgg gtattcttcc agataaccca
    16141 atttatcgag acgctgctgg tcacgtaatt tcacgcggca aaggttcaat acggcatcct
    16201 gcattgacat ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa
    16261 gtaaaccgat cagcaaatgc attccggtgt tgctgtcacc caacgctgca gcgcttacca
    16321 gcggcggacc atcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc tgagcaacgt
    16381 tttcataggc ttttacattc acataaggcg aacactcatc aaaccctttg atcgaaccaa
    16441 aaatcagacg tggattgatt tcttgaatat gctcccaggt gaagcccatg tgatcaatgg
    16501 cccctggatg aaagttctcg actaagatat cagcttcgcg gatcagcttt tccattacct
    16561 ctttgccttc cgctgttttg gtatttaact caatagaacg tttgttactg ttaagcatgg
    16621 tgaagtaaag cgcatcgata tcaggaatat ctcgcagttg gtgacgcgtt acgtcaccaa
    16681 cgccgggacg ttcaatttta ataacgtcag cgccaaacca ggccagcatt tgagtacaag
    16741 atgggccaga ttgcacaccg gtgaaatcga gaactttaat tccttgaagt ggagttgaca
    16801 tagcaatacc tattagttaa ttaaatatgt gttaatttag cgtttattta aggcataacg
    16861 ccctctataa ataatctgca aaatatcttc gtagcggcac tcgatatttt taaattcctg
    16921 tatcaagtac tgaaaattat tttatcgcca cttccaattc ctttctgcat ggtaagtagt
    16981 gaggacgaaa agaagaatat gataaatatt gattcgtggt aatcagagag agtcgcgaca
    17041 tttgtgatgt tattcttaca actgtaagaa gcgccggtga tttacaagta agagtatgat
    17101 tacgcttttt aaagcgtacc ataaagcata acataataaa tgatttgtga tgacacggga
    17161 ttattgcggt ttactttgag gaaaataaaa tgcgtgttta tagaaccgcg agcgccaaat
    17221 aattttatga ccacaacctg tcgtattgtg gtcataaaaa attgtcggct ggagggagtt
    17281 aaatccttta cctcaatata ctgcctgtca gcattagttc acaaacagtg cttcgctatc
    17341 gacgatgatt ccatcttccg cacctttaag cgccaggttt gcctcgtagt attgcttttt
    17401 atcaagcagt ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg gcatcagata
    17461 ttggttttcc attacgccaa caccggccag gcgaagctct tccattgcgc cctttttatc
    17521 ccctttcgcc attttttcat tggcaatttt tatcgcggct tctttttcgg gtgttgctac
    17581 ataactctca gaaattccga cagatgcatt aatgacaata tattggtcat cattgagatt
    17641 ggtcttttta cctggcttag cgaactttgc ccattcggta ctgtcatctg acagcaaagc
    17701 ggaagcttca ttggtcagtt cttttgcctt ttccggatcg ccatgaaaca gagccaggcg
    17761 cgctacctgc acgtcacgca tcgcatataa accttgttca gagatacgct ctgctgctgc
    17821 cgttttctgg gtttgctgag tttgctgttg tgccgctacc ggtgcattat cagcagccca
    17881 tacagttgaa gatgccagaa ttgctgtgac catcgtagcc ataattaaac gtttcatact
    17941 tacctcattc tttaacagtt ttagaaaacg ccgccatcat attgggtgag catgaaatga
    18001 gaaatcagag agcaacggtc attttgaaag ggttatctta caattgtagt tattatcgca
    18061 aagcggcatt tagtcttaca gttgtaggaa tatgcctcct gtcgcagaga actattctta
    18121 tataccacaa agagatgtca tttattatat tgatgtaaat attgattaat tatatgtaaa
    18181 tacagccata acatcgtatg gttctcagtg aaccattttc aggataactt tctatgaaag
    18241 taaacttaat acttttcagc ttatttttat tggtctctat tatggcatgc aatgtttttg
    18301 cattttccat ttctggtggt gtaagtgaga ggagctataa agagactgaa aaaacatcag
    18361 cgatgacgac cacacactct acaaaacttc agccatcaca ggcgattttg tttaagatga
    18421 gagaagatgt gccaccatta aacctcacag aagaaatcac gcctacttat ccgacaaagg
    18481 cgaattatct tattcatcct gtgcgatagc gtctgtcaca tggcaggtga acaatcatgt
    18541 ctgatgctga tgtgaacacc tgcccaaatg agccatcctg acccactatc gcctgccata
    18601 ccccaccata aagcgaatca tcaagccaca agccggggca agaaacataa atatccccgt
    18661 caccagttca ttaccgccag ttaaccacat ggtgaagtaa taaatgagtg cagcgaggat
    18721 caccatgccg aagaaagaag agaccgccga taccacgttc agtacctgac cgatgtagcc
    18781 gcttacgcag acaatacaca gggcgagaaa catccataaa taaatcatag ctcaacctcc
    18841 tgccgcgcta aaaaacctgt aacttcagtc actttaatag taagacaatc cttaacctct
    18901 ggcaattttt gctaagcatc aatgaaaaca gcacgttaaa tgcaacgcta ttgcgtgcgg
    18961 aaaatttttt actttgcacg attaataatc agtagctgaa agcagtcagc cagtgataaa
    19021 gtagagccac cgcattcatg ctcctcgtgg ttatgtcctg actagtcttt acactcttta
    19081 caggaaccat tgtcgtacat gatggcccaa ccaattgagt gtaccgctgt cgacatggaa
    19141 tcttctgcga tacaacaatt cgtatctaca gaaggtaact atgtttccac aatgcaaatt
    19201 ttcccgcgag tttctacatc ctcgctactg gctcacatgg tttgggcttg gtgtactctg
    19261 gctttgggta cagcttcctt atcctgttct ctgctttctc ggcacgcgta ttggcgcaat
    19321 ggcgcgacca ttcctgaaac gtcgtgaatc tatcgcccgt aaaaacctgg aactttgttt
    19381 cccgcagcat tctgcggaag aacgcgagaa gatgattgcc gaaaactttc gttcactcgg
    19441 catggcgctg gtagaaaccg gcatggcatg gttctggccc gacagtcgcg tacgtaaatg
    19501 gtttgatgtt gaagggttgg ataaccttaa acgcgcacaa atgcaaaatc gcggcgtaat
    19561 ggttgtcggc gtccatttta tgtcgctgga actgggcggc cgcgtgatgg gactgtgcca
    19621 accaatgatg gctacctatc gtccacataa taatcagctg atggaatggg tgcagacccg
    19681 tgggcgcatg cgctctaaca aagcgatgat cggcagaaat aatctgcgcg gcattgtcgg
    19741 tgcactgaag aaaggtgaag cggtatggtt tgctcccgat caggattatg gtcgtaaagg
    19801 cagctccttc gcgccgttct ttgcggtgga aaatgtcgcc acaaccaatg gcacctatgt
    19861 tctctcccgt ctctctggcg cagccatgtt gaccgtaacg atggtaagaa aagcggatta
    19921 cagcggatat cgtttgttca tcaccccaga gatggaaggc tacccgacag atgaaaatca
    19981 agccgctgct tatatgaaca agattatcga gaaagagatc atgcgcgcac cggagcagta
    20041 cctctggatc caccgtcgct ttaaaacgcg cccggtggga gaatcgtcgt tgtacattta
    20101 acaaactgct cttactcttt ccctcctcaa atcggccatg ataacatcat gatttttata
    20161 gctttttctt aagttttttc cttcgctgaa tatctaatta ttaccgtcag aaattaaact
    20221 gtcgccgata tgagacactt gtaagttact aaaagtaaat aagaaaatgc ctcttgtctc
    20281 gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt ttagctcaga attctggggt
    20341 gatgcccacg gtaaaataca gggcttttaa ggtcgctttg cccggtgtca actcactgta
    20401 tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg cggagagatg taaagtgaaa
    20461 tatttcttta tgggcatttc ttttatggtc atcgtttggg ccggtacttt tgccctgatg
    20521 atctaaaagc aaactgtgca atgaaaaaca ggagccatca ggctcctgtt tgcatttctg
    20581 acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct gctgacgggt agtaaaccgt
    20641 cggcacggaa catcgcttta atccctctga tggcctgacg aatacggtcg tggttttcaa
    20701 tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa gccaatcccc ggcgagacac
    20761 aaaccttcgc ttcgttaagg agcttcttgg caaattccag cgatcccatg gccgcatatg
    20821 gttccgggat tttcgcccag acatacatcg aagccttcgg catttcgacc atccagcccg
    20881 cttcatgcag ccctttaacc aatacatcac gacggcgttt gtactgttcg gcaatgtcgc
    20941 gcacgcactg ttgatcgccc tcgagtgccg caatcgccgc cacctgcaac ggcgtaaaag
    21001 tgccgtaatc gtgatagctt ttgattcgcg ccagggcact gaccagcgtt ttgttgccga
    21061 ccataaagcc gatacgccag cccgccatgt tgtagctttt cgacagcgta aagaactcga
    21121 ccgccacatc gcgtgcaccc ggcacctgca taattgacgg cgctttccag ccatcgtaga
    21181 cgatatcggc ataggccagg tcatggacca ccagcacatc gtagcatttc gccagcgcca
    21241 caaccttttc gaagaactcc agttccacgc attgcgcggt tgggttcgac gggaagccga
    21301 ggatcatcat cttcggtttc ggataacttt cacgaatggc gcgctccagt tcgttgaaga
    21361 aatcgacacc ttccaccagc ggcactgagc gtacctgcgc cccggcaatc accgcgccat
    21421 aaatatggat cgggtaactt ggattcggca ccagcaccgt gtcaccatga tccagcgtcg
    21481 ccagcatcag atgcgccagg ccctctttcg aaccaatagt gacgatggct tctgattccg
    21541 ggtcgatttc aacgtcgtag cgatcctgat accagcggga aatggcgcga cgtaaccgcg
    21601 gaatgccgcg tgaagtggag taaccatgcg tgtccgggcg ctgggccaca gtacataatt
    21661 tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc catgctgaaa tcgataatat
    21721 cttcgccgcg ccgacgcgca gccattttca gttcagcggt gatgttaaaa acgtagggcg
    21781 ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt gtcagccata atttcctcag
    21841 attaacgtta gcgcccggac cgtccgagcg acgctgccac aatggtggct gttttgaaaa
    21901 tagcctgatt aatttctgtc tgtccagcgt tttagaacat ttttatcaat ggatttaaat
    21961 gaggaactcc acttcaaaaa caggaaattt ttctacgcta acaagaaaat caccaccaat
    22021 tttattaaca ttataatttc aataaattta ccagccagaa gtgcattatc aagagataca
    22081 ttgctaatac accatttatc tttcaatccc cttttaaatt tatggttttt cctcaaaaac
    22141 ggcctgctgg tcatcagcct tcaggttacc tatcatagag gtttaatcct tattcagagt
    22201 cacccgtgca cgaaatattc aacatgctgc tggcggtctt cgatcgggca gcgttaatgc
    22261 ttatctgcct gttctttctc atccgtatcc gcctgtttcg cgaactgttg cacaagtcgg
    22321 cgcactcccc aaaagaattg ctcgccgtta ccgccatttt ctcgctgttc gccctgttca
    22381 gcacctggtc cggcgttccc gtagaaggct cgctggtgaa cgtacgtatt atcgcggtga
    22441 tgtccggcgg gattctgttt ggcccgtggg taggcatcat taccggcgtg attgcgggta
    22501 ttcaccggta tttaattgat atcggcggcg tgacggcgat cccctgcttt atcaccagca
    22561 ttctggcggg ttgtatatca ggctggataa acctgaaaat ccccaaagca cagcgctggc
    22621 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
    22681 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
    22741 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
    22801 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
    22861 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
    22921 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
    22981 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
    23041 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
    23101 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
    23161 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
    23221 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
    23281 tgagcaggcc cgctttggcg acaagctgac ggttatctat gatattgatg aagaggtgaa
    23341 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
    23401 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
    23461 acgcattgcg gtgcgagata ccgggcacgg catcgttcca aaggtgattg agcgggtcga
    23521 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
    23581 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
    23641 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
    23701 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
    23761 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
    23821 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
    23881 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
    23941 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
    24001 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
    24061 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
    24121 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
    24181 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
    24241 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
    24301 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
    24361 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
    24421 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
    24481 caacaatttt tcgccgatct gttcagcggc ctagtgctta acccgcaact actggggcgc
    24541 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
    24601 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
    24661 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
    24721 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
    24781 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga cccagcttcc cagcctgcaa
    24841 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
    24901 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
    24961 atgccgccgg gtaattcaca accgcgcggc gattttctct atcacagcat ttgtaactgg
    25021 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
    25081 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
    25141 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
    25201 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
    25261 cgtcagttta gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
    25321 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
    25381 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
    25441 atcccttcgt tagcacccag cgtcagcatg atcaccagtt cgacttcgcc catttctgac
    25501 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
    25561 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
    25621 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
    25681 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
    25741 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
    25801 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
    25861 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
    25921 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
    25981 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
    26041 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
    26101 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
    26161 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
    26221 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
    26281 cataagatct gatcgaggct gtgaaccatc gggatcatca actgggcgtt gccgaaactg
    26341 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
    26401 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
    26461 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
    26521 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
    26581 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
    26641 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
    26701 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
    26761 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
    26821 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
    26881 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
    26941 agaatcatac ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
    27001 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
    27061 caggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
    27121 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
    27181 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
    27241 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
    27301 aattgctgat tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
    27361 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
    27421 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
    27481 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
    27541 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
    27601 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
    27661 agcgagcttt tagcatcagc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
    27721 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
    27781 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
    27841 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
    27901 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
    27961 gtggcaggcc ccattaccaa tgtgggtacg ccagtaccgg ttaagtgcac cgctccaccg
    28021 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgccacg
    28081 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
    28141 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
    28201 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
    28261 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
    28321 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aactgaggcg atcatcaaag
    28381 gctttgccca tcacccgctg gtgagggagg acctgaaaag tggtatcgaa ggtgacgcga
    28441 tcgcctggac gaattcccgc ctgcatcact tcgtcatagg tgcgcgcgcc aatgtccacg
    28501 cgcatggcgc taacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
    28561 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
    28621 accggcagca cgtcaatagc gccttcgcgg gagatgctgc gcaccataaa tcccacttcg
    28681 tccatatggg cgcagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
    28741 gatcccagac catcaaatcg cacttctttt tgcaggcgag ccgcttcttc cagcaggatc
    28801 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
    28861 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
    28921 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctagca
    28981 aataaatccc cggctccacg gtcagcaaca tgcctggctg tagcgtcgtg gtgtcccgcg
    29041 gtgaaaaacg cggatcttca tggacttcaa tgccgatagc gtgaccagtg ttatgaccga
    29101 aatagtcgcc aaatccagcc tcggtaatga cccggcgcgc ggcgtcgtca atctgctggc
    29161 agcgcacgcc ggggcgaatt gcagagattg ccgcgagctg tgcctgcagg acaatctgat
    29221 agacgtcaaa cagcgggtga gattcggcgc tcaccccttc gccattcacc agcaaggtgc
    29281 gcgtcatatc agcagtagcc ctgatacagc gcaccaaaat cgagagtgac aaactcgccc
    29341 gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccgcgcca gccgctggcg
    29401 acaatggtgt caaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca
    29461 gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctccgctccg
    29521 cgatcggcaa tcccacaggc gaggcggatt atctccacct cctctggcgt tttgatttgc
    29581 cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactt ccagcggtgc
    29641 gcggtttccc aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca
    29701 atgatttgat tgacgatagt ggtaagcgtg ttcgtcgcgt caagcaaatg cagctggtag
    29761 ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt
    29821 tcacggctaa taaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc
    29881 tgccgtgagg agagaagcac cgcatccagt tgttgcacct taagccagtt gcgcagcgaa
    29941 gcgagtaatg tcatctggat tttccttctt acaggctatc gattaacaat ttgcctttgc
    30001 ggaacatcat cagacgcagg aacaccacca tcagcgcagt aatgattgcc cccagcgcga
    30061 tccccgccat ataaacgccc aggttagtga ccagcggcca tgcccagata gcggattccg
    30121 ggaaccattg cactgcgccc agccagacag ctgcggttga gccgacaatc gcgccgacca
    30181 tatatgacgg aatagcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactaa
    30241 tccccataaa ggccaggaac atcgcggtct taccctgcgg ataaagttga gcgttgaaca
    30301 ggcgtttgcc ggttaaacga cggtcaataa tggtcgccag acccagacca atcggcggaa
    30361 taacgatagc gatagaacga gcggtgaccg gtaatacgtg gtcagtggta aagctgaagg
    30421 caacgaaacc tgcggctttg ttgatcgggc caccaaggtc gattgctgtc gccgcggcga
    30481 tccccattgc gtacatcagc gcgcccttct cacctgcggc agtcagcaca gtacggatac
    30541 cgccgttgat ccagccacca aagggagtga tgacgtagta catcgccagc atgacaaaaa
    30601 tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact
    30661 ggattttctg gttcatccat ttcacaaggt aaccggcaac gatagaaata atcagcgcac
    30721 caatgaaggt ggacggcacc ggcgaagagg tcgcccattg catagtgctg ggatcgaagt
    30781 tcagcagttg cgtcggctgg gtagacatca acccgccgat aaaaccagcc gggaacgcca
    30841 gtttgccacc gatagagttc gccacaaatg cagcaaacat cggaatggca aaaccaaaca
    30901 acacgccgcc gaaggactgc gacagccagg caaatttaag taaggagagg tcgaaaccgg
    30961 agaatttccc gctattaaga gcatccatga tgccgatctc agcaggaatt ttaagccagc
    31021 tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca
    31081 tgcgtgaaat ccctgacatc acatgctgcg ggagttcacc ccagaagctg cttttagagg
    31141 cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc
    31201 gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca
    31261 tctcttcgat ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt
    31321 cataaacgtc gcgtgattcg aaacgttcgt tatcttccgg ggtaactgcc acggaatgga
    31381 tgatgatggt cgcttcggcg atatcctgcg ccgtcaggcg attctggatg ccgtccgcgc
    31441 cctgggtttc aattttcact tcataaccgg cttctaccgc cgcttcttcc agcacctgag
    31501 cggccataaa agtgtgagct aggcccattg ggcaggcaca taaggcaatc agtttcttac
    31561 tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa
    31621 atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg
    31681 tgacgcaggt cggccttgtg aggggacaca aagaaaaatt gaattaactg tatgatttaa
    31741 aagattatcg ggagagttac ctcccgatat aacaggaagg atttacagaa tgtgacctaa
    31801 ggtctggcgt aaatgtgcac cggaaccgag aagacccgga tagtcatgga cgatgagata
    31861 caccggaata tcatggacat attctttaaa gcgcccttta tcttcaaatg cggcacggaa
    31921 accggaggct ttgaagaact caaggaagcg cggcacgata ccgcccgcaa taaacacgcc
    31981 gccaaatgtc ccgagattga gcgccagatt gccgccaaaa cggcccataa tgacgcaaaa
    32041 cagcgacaat gcgcggcggc aatcggtgca gctgtcagcc agcgcgcgtt cggtaatatc
    32101 ttttggcttg agattttctg gcaggcggtt gtcagctttc acaattgcgc gatacaaatt
    32161 caccagccca gggccagaaa gcacgcgctc cgccgaaaca tgaccaattt ccgcacgcaa
    32221 tatttcgagg ataatggcct cttcttcact attcggcgca aaatcaacgt gaccgccttc
    32281 gcccggcaag cttacccaac gcttatcgac atggaccaga tgcgcaaccc caagccccgt
    32341 tccggcaccg taaaccgcaa taggcttacc ttcgaccggt tctgcgccac caaactgaat
    32401 cagatgctct tttttcagca tcgggatcac catcgataca gcggtaaaat cgttaataat
    32461 ttccagatgg ctaaaaccga gattcttttt catttcggca attgagaacg cccaggtatg
    32521 gttggtcatt gccacccagt caccggtaat tgggcaagcg atggcaatac agccgtcttt
    32581 cacctcgacc ttatgttctt caagataaac gcgaatgacc gcttcgaggc tggggtaatc
    32641 aagccctgaa taggtcttag cctgcgagat ttcaccactg gcaatatcac acagagcaag
    32701 acgtgcgttg gtgccgccca catcaccgac taatgcatac tttgtcattc ttcaactgct
    32761 ccgctaaagt caaaataatt ctttctcaca ctgtaaatac ctgggggcat aacaacaacg
    32821 ccggaaaggc aggctccctg taaatatcga tctgggtcac acaattactt tatcgtttca
    32881 gcaccaattg cagcgatgcc tttttgcaag ctgggcaaac taagtatctg accccgcata
    32941 aggaatagaa catgctccat ccgcgagcca gaaccatgtt gttattatcg ctccccgccg
    33001 tggcaattgg gattgcgtcc agtcttattc tgattgtggt gatgaaaatc gcctcggtat
    33061 tacagaattt gctctggcaa cgactgccgg gaactctggg gatagcccag gattcacccc
    33121 tctggatcat cggtgtatta acgctaacgg gtattgcggt ggggttggtt atccgtttca
    33181 gccagggtca tgccggacca gaccccgcct gtgaaccgct gatcggcgca ccggttccgc
    33241 cctctgcgct acctggactt atcgtagcat taattctcgg tcttgctggc ggcgtcagcc
    33301 tggggccgga acatccgatc atgaccgtca atatcgccct tgccgtcgcc attggcgctc
    33361 gtctgttacc gcgcgtcaac cgaatggagt ggactatttt agcctctgcc ggaaccatcg
    33421 gcgcgctgtt tggtacacct gttgcggcgg cgttgatatt ttcgcaaacc ttaaatggca
    33481 gtagtgaagt tccgctatgg gatcgtcttt ttgcgccgtt aatggcggca gcagctggtg
    33541 cacttactac cggattattt ttccatcccc atttttcact gcccattgcg cattacggac
    33601 agatggaaat gaccgatatt ctcagcggtg caattgtcgc ggcgattgcc atcgcagcag
    33661 ggatggtcgc cgtatggtgc ttaccacggt tgcacgcgat gatgaatcaa atgaaaaatc
    33721 cggtgctcgt gctgggtatt ggcggattta ttctcggaat tctgggggtt attggtggac
    33781 cagtttcgct gtttaaaggg ctggatgaga tgcagcagat ggtggcaaat caggctttca
    33841 gcaccagcga ttactttttg ctggcggtaa ttaaacttgc cgccctggtc gttgctgccg
    33901 ccagtggctt tcgcggtggg cgaatcttcc cggcagtgtt tgtcggcgtg gcattagggt
    33961 tgatgctgca tgagcacgtt cccgccgttc cagcggcaat aaccgtttcc tgcgctattc
    34021 tcggcatcgt gctggtggta acacgcgatg gctggttaag tctttttatg gcggcagtcg
    34081 ttgtacccaa taccacattg ctaccgctgc tctgtatcgt catgcttccg gcatggctgt
    34141 tattagcagg taagccgatg atgatggtca atcgtccgaa gcaacagcca ccccacgata
    34201 atgtttagca aaaaatgctc tcctttatga tttaagagtt atggcgatga tacggggctt
    34261 tacctccccg taatattgcg ttaacaggcc gctgacggct tcgatcgtga aggagaataa
    34321 caatgttcag gtcactgttt ctggcggccg ccctgatggc atttaccccg cttgcagcaa
    34381 atgcaggtga aatcacccta ctgccatcga tcaaattaca aattggcgat cgcgatcatt
    34441 acggtaatta ctgggacggt ggtcactggc gagatcgtga ttactggcat cgcaattatg
    34501 aatggcgcaa aaaccgctgg tggcgtcatg ataatggcta tcatcgtggc tgggataagc
    34561 gtaaagcata tgagcgtggc tatcgtgaag gctggcgcga tcgtgacgat catcgcggaa
    34621 aaggccgcgg acatgggcac cgccattaaa cgcgtcctcc aatggagcac aatgcctgat
    34681 gcgacgctag cgcgtcttat caggcctaca aaccggctct ttaacgcgta gggcggataa
    34741 ggcgtttacg ccgcatccgc caatagtgcc agatgcgacg ctcattcaac tacaatccca
    34801 gcgccgtccc caccaacaac cagatattca gcgccacgac cagcactaca atcacccagc
    34861 ctgtctgttt tacgcgtttg ctgttcacca gatcgcccat caacttgctg tcactggtga
    34921 aaatcagcag tggaaccagc gccagggcga taccaaaact taacagcacc tgactcataa
    34981 ccagaatccg tgtcggatct aatcccatca gaatgacaat aaatgacggc aacatggtga
    35041 ctgtacgacg cacccacagc gggatatgga agcgaatgaa tccctgcatc accacctgcc
    35101 ccgccagtgt ccccaccacc gttgaggaca gtccggcagc aaccagactt aacccaaaga
    35161 ccgttgccgc agcatggctt aacagcggtt gcagcgtcag ataagcctca tcaagatcgg
    35221 caacaccagt atgaccagaa aagtggaacg ccgccgcagc tgtagccatc atcaccagat
    35281 tgacaaaacc ggcaatcgtc atggcgatag ccacatccca tttggtggcg gaataacgtt
    35341 gttgacgcga accgccatgt aaatgctgag tgagcgaaga gtgcaaataa atcacatgcg
    35401 gcataatcgt cgcccctaac acgcctgctg ccaggaagac cgcctccgaa gtaggtaaac
    35461 tcgggatcac cattccttta cccagctgcg ccaggttagg ctgggagaaa atcaactcga
    35521 caatgtaagc cgcggcaaca aacaacagta acccgccaat cactttctcc agcggttttt
    35581 gcccgcgacg ttgcagcatt aaaatcagga aagtcgcgat ccccgtcagc accgcgccct
    35641 gcaacaacga aacaccaaga atgagtttaa aaccgatcgc cgcaccaata aattccgcca
    35701 ggtcggttgc catcgcaata atttctgcct gaacccaata gaaccacact acgggacgcg
    35761 gatagtgatc acgaatctgc tccgccagat ttttaccggt ggcaatccct agtttggcag
    35821 agaggatctg aatcagcatc gccatcaggt tggcccaaac gacaacccac agtagctgat
    35881 agccgaagct agcacccgcc tgaatattgg tcgcaaagtt accgggatcg atataaccaa
    35941 tcgccgcaat gaacgcaggt cccattaatg cgagcctcat cttgcgcgcc gcccgtccgc
    36001 tgctactctc aacgcgatag ttcgtcatct tgtgcctcta aaacatagcc tttgctatgt
    36061 ttcatgctat gctaaacgag aatgattatc aaattcattt aaatggattg tggtgatttc
    36121 tctgatagac caagattatg attacgaaaa gattgatgac ttgaatgtta tggttatgtt
    36181 taatgttagc acatttacat aactttcagc ttccatacac aacatagcag aaatgtatga
    36241 cagatcacta tttttgaagc ctgtcacagg acgtcattat agtgtgtgtc agatctcgtt
    36301 ttccttaacc atgttacata gaatgtgcac ggaaatttaa cctgcctcat atttggagca
    36361 aatatggacc gcgtccttca ttttgtactg gcacttgccg ttgttgtgat tctcgcactg
    36421 ctggtaagca gcgaccgcaa aaaaattcgt atccgttatg ttattcaact gcttgttatc
    36481 gaagtgttac tggcgtggtt cttcctgaac tccgacgttg gtttaggctt cgtgaaaggc
    36541 ttctccgaaa tgttcgaaaa actgctcgga tttgccaacg aagggactaa cttcgtcttt
    36601 ggtagcatga atgatcaagg cctggcattc ttcttcctga aagtgctgtg cccaatcgtc
    36661 tttatctctg cactgatcgg tattctccag cacattcgcg tgttgccggt gattatccgc
    36721 gcaattggtt tcctgctctc caaagtcaac ggtatgggca aactggaatc ctttaacgcc
    36781 gtcagctccc tgattctggg tcagtcagaa aactttattg cctataaaga tatcctcggc
    36841 aaaatctccc gtaatcgtat gtacaccatg gctgccacgg caatgtccac cgtgtcgatg
    36901 tccatcgttg gtgcatacat gaccatgctg gaaccgaaat acgtcgttgc tgcgctggta
    36961 ctgaacatgt tcagcacctt tatcgtgctg tcgctgatca atccttaccg tgttgatgcc
    37021 agtgaagaaa acatccagat gtccaacctg cacgaaggtc agagcttctt cgaaatgctg
    37081 ggtgaataca ttctggcagg tttcaaagtt gccattatcg ttgccgcgat gctgatcggc
    37141 tttatcgccc tgatcgccgc gctgaacgca ctgtttgcca ccgttactgg ctggtttggc
    37201 tacagcatct ccttccaggg catcctgggc tacatcttct atccgattgc atgggtgatg
    37261 ggtgttcctt ccagtgaagc actgcaagtg ggcagtatca tggcgaccaa actggtttcc
    37321 aacgagttcg ttgcgatgat ggatctgcag aaaattgctt ccacgctctc tccgcgtgct
    37381 gaaggcatca tctctgtgtt cctagtttcc ttcgctaact tctcttcaat cgggattatc
    37441 gcaggtgcag ttaaaggcct gaatgaagag caaggtaacg tggtttctcg cttcggtctg
    37501 aaactggttt acggctctac cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg
    37561 ctgtaaaacc atacataaaa aagccgggga tcattccccg gcttttttac gcctgttaat
    37621 cagctaatgg ctgcgggcga ccaatcaaat acccttgcag atattgcacc ccgagcttat
    37681 gcaatagcgc ctgctgctgt tgcgtctcga caaactccgc gaccacactc aatgacttcg
    37741 ctttcgccag atcggtaatt gatctcacaa tcatcgcatc cagcgtattc gtgacaatat
    37801 ctttcacaaa gacgccatca attttgatga tatcagcctg caaacgcttt aaccgttcgt
    37861 agttggcata tccggtgcca aagtcatcaa tcgcaatccg gaaaccaaac ttatgcagct
    37921 gctcgatgtt gtacatgctg ctttctgcgt tagaaaacgc ctgctcctcg gtgatctcaa
    37981 gaatgaccgc ctgcggggag atgtgataac gcttaaacag acgaataatc cgcccggcaa
    38041 tattcttttg cagcagcgtg agcggcatta aattgactga aaagcgcggt ccttttttgt
    38101 cgcaagggtg tgttgccagc cacttcaaca aggattccag cacttgcaaa tcaaaacgcg
    38161 cgctaaggtt aaactgagca ataaggggca gaaacttatc cggggtcata atgccgccgt
    38221 catatttcag tcgcgcgagg atctcatcat aaccttcacc ctctttgttg cgaattggct
    38281 gggcgtagag cagcaaatca ccctgatcta acgccgtgcg aatggtattc agcaatagca
    38341 cctgtttagt ggtctgcccg gaaaccatct cctctctgct atccagcgcc agcacatgat
    38401 gatgtgcgca ggattgctcc gccagccagc ttaactgccc caacaagggt tgcagggttt
    38461 cctgatttcc atcaaaacgc ccccaggcag caccatagcc catatccagc ccggtattgt
    38521 tccagtgaat ttgccgacta ttcaggatgt taaccatatg ctggagtcgc ccttccgttt
    38581 ccggcccgct cagcactaac agcagttcac tacccggcaa ttgatacaac ttttcgtttt
    38641 cctgcatcaa cggcagcagc gtacggcaaa gccgtaatga cgactcataa actcaagatt
    38701 atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc
    38761 acgaaagttg ggtaggagcg ttaacggatc tgttagcgcc tgcaaatgcc agcggcgatt
    38821 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
    38881 cagcaccgcg agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
    38941 gttgtaattc agaaggcaaa gcgttgaaac agcccaggtg agatttaaaa acggatagcg
    39001 aagattaccg accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat
    39061 aaagtcgttt tcataaggtg tgcacaacag aagtagcagc acgcttaacg ctgccagcca
    39121 gcttaaggta aatgcgagtg agatagtaga aaagcatgtt gtaaatcagc actgcggtga
    39181 aaaggcttag taaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata
    39241 tctttagcgg gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg
    39301 gcgtcaccaa accaagccag aacagacgtt gccagacata tcggctggtc aatccataac
    39361 gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct
    39421 ggctgccgag catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc
    39481 aaaacatccc gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca
    39541 gcattacgca cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg
    39601 gcgaaataaa acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata
    39661 tcttgatatt ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg
    39721 gggttttcag gcacacaaat aataagttta ttcgcatcgc gaagcaaaca ggggccagcc
    39781 tgttgccgat ttttcatcca atgatgcttt caccaggcgg cgctaacccg gacgtagact
    39841 gtattacaaa agcggcaaaa agcagagaca aaaaaccccc gctttgcagc gagggttgga
    39901 aatttggtgg agctaagcgg gatcgaaccg ctgacctctt gcatgccatg caagcgctct
    39961 cccagctgag ctatagcccc acgatgcgtt tacgtaccaa atttgctggg tgcaaaattt
    40021 ggtggagcta agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc gctctcccag
    40081 ctgagctata gccccgtcac gtaaagcttg tcgagttgac gggcggcatc atatgaattc
    40141 cgcccgaatg tgtcaacggc aaattgcaac gtgtagtttc aatcgctgaa aaatcaggca
    40201 aatgaacaat tttggaaaac cgctcgcatt cagtagttat tcatgtcacg gtttcctgta
    40261 aagtggtgtt ataaaatgaa ctactaatag acccacatac attcagggaa ttgttatgtt
    40321 caaggagagg atgacaccag atgaacttgc cagactgacc ggctatagcc gccagaccat
    40381 taataaatgg gtacgcaagg aaggctggac aacgtcacca aaacctggcg tccagggtgg
    40441 caaagcccga ctggttcacg tcaatgaaca ggttcgtgaa tatattcgca atgctgaacg
    40501 tccagaaggt cagggagaag cgcctgccct ttctggtgat gcgccgcttg aagtattgct
    40561 ggtgacactg gcgaaagaga tgacgccagt tgaacaaaaa cagtttacat ccttgcttct
    40621 gcgggaaggg attatcggat tgttacaacg cttagggatt cgcgatagca aataatatga
    40681 aaagattacg caataaaatg accaccgaag aactggctga atgcctcggt gtggctaaac
    40741 aaactgttaa ccgttggacc agagaaaaag gctggaaaac ggaaaaattt cccggcgtga
    40801 aaggcggtcg tgccagactt attctggtcg atacgcaagt ttgcgagttt attcagaaca
    40861 cgccagcctt ccataacaca ccaatgttga tggaagctga agagagaatt gctgaatacg
    40921 ctcctggagc acgcgccccg gcgtatcgac aaatcatcaa cgcgatagat aatatgacag
    40981 acattgagca ggaaaaagtc gcgcaatttt tgtcacgtga aggaattcgc aatttcctcg
    41041 cccgtctcga tattgacgaa tcagcataaa taaaaaacgg caggatatta tctcctgccg
    41101 tttatctttt tacacgctaa ttactgctga ttttcgcgtt cagcaataaa atccagcgct
    41161 ttgttgatac gctcgatact gcgggtctta ccaattgcgt gaacggtaac atccagtgct
    41221 ggagactgcc ccgcaccggt tacggcgaca cgcagcggca taccaacttt acccataccc
    41281 acttccagct catccgccgt cgcctgaata gcgtgatgaa cgttttcagc ggtccagtca
    41341 gtaatcgcgg ccagtttgtc acgaaccact tccagcggct gacgcgctac cggacgcaga
    41401 tgttttttcg ccgcatcggc atcgaactca gcaaaatctt cgtagaaata acggcagctc
    41461 tgtgccatct ctttcagcgt cttgcagcgt tcgcccagca gtttcaccag atcagccagc
    41521 tgcgggccgt tacgggtatc gatattttcc tgctcaatgt gccactgtaa gtgagtagca
    41581 acatactccg gcggcagcgc gttaatgtag tgatggttca gccacagcag cttgtcggtg
    41641 ttgaacgcac tggcagattt gctgacggca ttcaaagtga agtatttgat catctcttca
    41701 cgagtgaaga tttcctgatc gccgtgggac cagcccagac gcaccagata gttcagcagt
    41761 gcttctggca aataaccgtc atcacgatac tgcattacgc tgactgcccc gtgacgtttg
    41821 gacagttttt taccgtcatc gccattgatc atagaaacgt gcgcgtaaac cggcaccggc
    41881 gctttcaggg ccttaagaat gttgatctgg cgcggcgtgt tgttgatatg gtcttcgcca
    41941 cggataacgt gggtgatttc catatcccag tcatcgacaa ccacacagaa gttataggtt
    42001 ggggaaccat cggtacggcg gataataaga tcgtccagtt cctggttgct gaactcgatc
    42061 ggaccacgga tctgatcgtc aaaaacaaca gaaccttcct gcgggttagc aaaacgtaca
    42121 acacacggtt catcatcagc atgatgctca tggctgtggc ggcagcgacc gtcataacgc
    42181 ggcttctcac ctttcgccat ttgctcttcg cgcagcgctt ccaggcgctc tttagagcaa
    42241 tagcatttat aagcagtgcc ctcttccagc atctgatcga tcaccgcgtt gtagcgatca
    42301 aaacgtttgg tctggtagta cggaccttca tcccactcca ggctcagcca gttcatgcca
    42361 tccataatgg cttcgatagc ttccggcgtg gaacgctcaa gatcggtgtc ttcaatacgc
    42421 agcacgaacg caccgccgtg gttacgtgca aaaagccagg agtaaagagc agtacgcgcg
    42481 ccgccaacgt gcagatagcc tgttgggctt ggcgcgaagc gagttttgat tttcatgaaa
    42541 tggccttacg tttagaaaga tgccgacaac cggcaaatcc tggaaaaatt gagtgggcga
    42601 tattctatca ctcccgcctg attcctcaat gtagttcggg tttctacctt acgcattatt
    42661 gttttcgttg acaaattgcg caacacggtc tgttttgcga tcatcttgct taaatttacg
    42721 acgaacgaac aatttcttta gaaaatgcgt tgactcattt tgaactctcc ctataatgcg
    42781 actccacaca gcgggggtga ttagctcagc tgggagagca cctcccttac aaggaggggg
    42841 tcggcggttc gataccgtca tcacccacca actactttat gtagtctccg ccgtgtagca
    42901 agaaattgag aagcgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg
    42961 tcggcggttc gataccgtca tcacccacca cttcgggtcg ttagctcagt tggtagagc
//
LOCUS       DAJVPP010000025        40211 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVPP010000025 DAJVPP010000000
VERSION     DAJVPP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.025"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..615)
                     /locus_tag="OQM34_001330"
     CDS             complement(<1..615)
                     /locus_tag="OQM34_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCS1451844.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIH"
     gene            861..1487
                     /gene="gstB"
                     /locus_tag="OQM34_001331"
     CDS             861..1487
                     /gene="gstB"
                     /locus_tag="OQM34_001331"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCS1451845.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1484..2598)
                     /gene="yliI"
                     /locus_tag="OQM34_001332"
                     /pseudo
     CDS             complement(1484..2598)
                     /gene="yliI"
                     /locus_tag="OQM34_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2709..3092)
                     /gene="bssR"
                     /locus_tag="OQM34_001333"
     CDS             complement(2709..3092)
                     /gene="bssR"
                     /locus_tag="OQM34_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1451846.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3305..4630
                     /gene="rimO"
                     /locus_tag="OQM34_001334"
     CDS             3305..4630
                     /gene="rimO"
                     /locus_tag="OQM34_001334"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1451847.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4677..6005)
                     /locus_tag="OQM34_001335"
     CDS             complement(4677..6005)
                     /locus_tag="OQM34_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1451848.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6013..8361)
                     /locus_tag="OQM34_001336"
     CDS             complement(6013..8361)
                     /locus_tag="OQM34_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1451849.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8539..9450)
                     /gene="gsiD"
                     /locus_tag="OQM34_001337"
     CDS             complement(8539..9450)
                     /gene="gsiD"
                     /locus_tag="OQM34_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1451850.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9453..10373)
                     /gene="gsiC"
                     /locus_tag="OQM34_001338"
     CDS             complement(9453..10373)
                     /gene="gsiC"
                     /locus_tag="OQM34_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1451851.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10391..11929)
                     /gene="gsiB"
                     /locus_tag="OQM34_001339"
     CDS             complement(10391..11929)
                     /gene="gsiB"
                     /locus_tag="OQM34_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1451852.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(11949..13820)
                     /gene="gsiA"
                     /locus_tag="OQM34_001340"
     CDS             complement(11949..13820)
                     /gene="gsiA"
                     /locus_tag="OQM34_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1451853.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13807..14772)
                     /gene="iaaA"
                     /locus_tag="OQM34_001341"
     CDS             complement(13807..14772)
                     /gene="iaaA"
                     /locus_tag="OQM34_001341"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1451854.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            14976..16211
                     /gene="moeA"
                     /locus_tag="OQM34_001342"
     CDS             14976..16211
                     /gene="moeA"
                     /locus_tag="OQM34_001342"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1451855.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16211..16960
                     /gene="moeB"
                     /locus_tag="OQM34_001343"
     CDS             16211..16960
                     /gene="moeB"
                     /locus_tag="OQM34_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1451856.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17139..17801)
                     /gene="fsa"
                     /locus_tag="OQM34_001344"
     CDS             complement(17139..17801)
                     /gene="fsa"
                     /locus_tag="OQM34_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1451857.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            17932..18831
                     /locus_tag="OQM34_001345"
     CDS             17932..18831
                     /locus_tag="OQM34_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1451858.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            18837..21269
                     /locus_tag="OQM34_001346"
     CDS             18837..21269
                     /locus_tag="OQM34_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1451859.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21415..22230
                     /gene="ybiV"
                     /locus_tag="OQM34_001347"
     CDS             21415..22230
                     /gene="ybiV"
                     /locus_tag="OQM34_001347"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1451860.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22382..23647
                     /locus_tag="OQM34_001348"
     CDS             22382..23647
                     /locus_tag="OQM34_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1451861.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(23888..25480)
                     /locus_tag="OQM34_001349"
     CDS             complement(23888..25480)
                     /locus_tag="OQM34_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS1451862.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25699..26619
                     /gene="ldtB"
                     /locus_tag="OQM34_001350"
     CDS             25699..26619
                     /gene="ldtB"
                     /locus_tag="OQM34_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1451863.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26677..27795)
                     /locus_tag="OQM34_001351"
     CDS             complement(26677..27795)
                     /locus_tag="OQM34_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1451864.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQSVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27792..28259)
                     /gene="mntR"
                     /locus_tag="OQM34_001352"
     CDS             complement(27792..28259)
                     /gene="mntR"
                     /locus_tag="OQM34_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1451865.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28445..28573
                     /gene="mntS"
                     /locus_tag="OQM34_001353"
     CDS             28445..28573
                     /gene="mntS"
                     /locus_tag="OQM34_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1451866.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            28845..30428
                     /gene="opgE"
                     /locus_tag="OQM34_001354"
     CDS             28845..30428
                     /gene="opgE"
                     /locus_tag="OQM34_001354"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1451867.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30476..30991)
                     /gene="ompX"
                     /locus_tag="OQM34_001355"
     CDS             complement(30476..30991)
                     /gene="ompX"
                     /locus_tag="OQM34_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1451868.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31044..31109)
                     /locus_tag="OQM34_001356"
     CDS             complement(31044..31109)
                     /locus_tag="OQM34_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451869.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31344..32231
                     /gene="rhtA"
                     /locus_tag="OQM34_001357"
     CDS             31344..32231
                     /gene="rhtA"
                     /locus_tag="OQM34_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1451870.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32530..33033
                     /gene="dps"
                     /locus_tag="OQM34_001358"
                     /gene_synonym="pexB"
     CDS             32530..33033
                     /gene="dps"
                     /locus_tag="OQM34_001358"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1451871.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33437..34183
                     /gene="glnH"
                     /locus_tag="OQM34_001359"
     CDS             33437..34183
                     /gene="glnH"
                     /locus_tag="OQM34_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1451872.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34322..34981
                     /gene="glnP"
                     /locus_tag="OQM34_001360"
     CDS             34322..34981
                     /gene="glnP"
                     /locus_tag="OQM34_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1451873.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            34978..35700
                     /gene="glnQ"
                     /locus_tag="OQM34_001361"
     CDS             34978..35700
                     /gene="glnQ"
                     /locus_tag="OQM34_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1451874.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            35961..38185
                     /gene="ybiO"
                     /locus_tag="OQM34_001362"
                     /pseudo
     CDS             35961..38185
                     /gene="ybiO"
                     /locus_tag="OQM34_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38182..39108)
                     /gene="rlmF"
                     /locus_tag="OQM34_001363"
     CDS             complement(38182..39108)
                     /gene="rlmF"
                     /locus_tag="OQM34_001363"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1451875.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39384..39644
                     /locus_tag="OQM34_001364"
     CDS             39384..39644
                     /locus_tag="OQM34_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1451876.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            39909..>40211
                     /locus_tag="OQM34_001365"
     CDS             39909..>40211
                     /locus_tag="OQM34_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1451877.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT        10015 a        10629 c         9789 g         9778 t
ORIGIN      
        1 atgaatggcg tactcttccg gcacatcgtg gatcaatgct ttacccagca atgccatatc
       61 gcgcgcggta ctgaactgcc ccggcgcatc caggccgtgc accgtctgga aggtagtgtt
      121 ggtcagcccc agttttttgg cataaccatt catcagacca ataaaggact cctggctccc
      181 ggcaacgtaa tctgccagcg caatacaggc gtcattaccg gactggataa tcacaccttt
      241 gttcaagtct gccaccgaaa cctgatcgcc cggtttgagg aacattaccg atgaaccacg
      301 cagtgccgga tttcccgtcg cccaggcatc tttaccgacc gtcaccatat cggtgagttt
      361 aatcttatcg gccttaagcg cctgcccaac cacatagctg gtcatgattt tagtcaggct
      421 cgcaggatcc agtttctcat ccgcattgcc ttctgccagc actttaccgc tggcgtaatc
      481 cattaaaatc catgcacgcg catccacgct cggcgcttca acggtttgtt cggccgcgaa
      541 tgccgttggg gcaaaaagga ataaaaatgc agaacccgct gcaagaccac gaaggagaga
      601 ggagtattgc gtcataaatg ccacccgagt atccattcaa aaaaatacgt tatatcgccg
      661 tatgataagt tcggtgatta ataacgcaca cacaaactta aagaaaccgt aagttggtaa
      721 agtttttaaa gtttatgcaa tatcctgccg cttcgctgct tctggtgtca tttttttgcc
      781 agctgttgct taaaagttaa ctttacttca ccatcggagt cctgttcagt ctgtggctgc
      841 ctgtcggaaa aggggtgagt atgattacgc tgtggggtcg gaataattca actaacgtaa
      901 aaaaagtatt gctgacgctc gaagaactgg aactacctta tgagcaaatt ctcgcgggcc
      961 gtgagtttgg gataaatcac gatgctgatt ttctggcgat gaaccctaac gggctggtgc
     1021 cgttgttgcg tgacgacgaa agtgatctca ttctttggga atcaaacgcc attgtccgct
     1081 atctggcggc acagtacggg caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg
     1141 aagcggaaaa atggatggac tgggcaaacc agacgctcag taatgctcat cgcgggatcc
     1201 tgatgggatt agtcagaaca ccaccggaag agcgcgatca ggccgccatt gatgccagtt
     1261 gcaaagagtg cgacgccctg tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt
     1321 tctctggcga cgagtttggt gtgggcgata tcgctatcgc accgttcatc tacaatttgt
     1381 ttaacgttgg cctgacctgg acaccgcgtc caaatctgca acgctggtat cagcaactca
     1441 ctgaacgccc cgcagtgcgc aaagtggtga tgattcccgt tagctaattg cgtgggctaa
     1501 ctttaagtaa ttccccactg gactcgtcgg tgagaacgta taaataaccg tcgggtccag
     1561 tgcgaacatc acgaattcgc tgccctctgt ccgttaaaat acggccatct tctgtcactt
     1621 tgtcgccgtt gacgctcatc acaatgacat ctttatcttt cagctcccaa taaataattt
     1681 ttgctgccac tgggggaatt tatcgctgtt atagaaggcc atgccgctca cagcgggcga
     1741 atctttccag taaaaaacag gttgctcggt cccggcgacg atctcccctt tcgcttccgg
     1801 tatcttaaag cctgaatagt tgattcccca ggttgccagc ggccagccgt agtttttgcc
     1861 tttttgcggg atattaattt catcaccacc gcgcgggcca tgttcattca gccacagtgc
     1921 attactccac ggattcatcg ccattccttg cggattacga atgccataag accagatctc
     1981 ggcgcgcgca ccagattcct ttataaaagg attatcatcc gggatttcgc cctggtcggt
     2041 cagacgcacc agtttgccct gtaatttatc cagatcctgc gccgtcgggc gctgattgtt
     2101 ttcgcccaga gcaataaaaa gataaccttt accgtcgaat accagccgcc cgccaaaatg
     2161 gttgccggta gacagttttg gcatctggcg gaagacggtg cggaagtcgg tcacttttga
     2221 gagatcatca cttaagcggc cataacccac agcagttccg gctttgccat catcgccaac
     2281 ttcggaataa cttaaccaga tgcggcgaga ctgagcaaaa tcaggcgcta aaaccacgtc
     2341 cagcaggccg ccctgcccgt gcgcccaaac gtccggaact ccggaaagcg gcgcagataa
     2401 tccttttcct gcttgccagt gacgcaactc gccgccgcgc agagtgatta acataccgtg
     2461 attatcgggt aaaaaggcca gtgcccaggg atggtcgagt ttgtcttgca gtacttcgac
     2521 atttaccgtt gcaggagccg cccagagagc ggaagaaaga caaataaggg gcacaaggaa
     2581 aaaggattgt cgatgcataa cgcctccgga ttgactctgg cttaaagcgt agtcagtgga
     2641 ggagataaca aattcatttt tacaaaaact taaacatgaa gggggagacg ctttctcccc
     2701 cttagttttc aggccttctc aagcatggcg tgcttctgca ggctctggat actcagcgtt
     2761 aagctcatca gacaattttc aagcttatcg gcgttgacgg taataacagt cgggcaatca
     2821 tggtgcccac tcatcaaaca tactgcggct gtcgctaatg cttcttcagc atgatgaaga
     2881 gcactccact cttcctgatc cagatgaaga ttcaaccgca gcgatttatc gtgcagttcg
     2941 cgattcagtt taaaaaagtt atctcgtaga tgattgcttt cgctgacgga catgtatcct
     3001 tttgccttcc tcagttgcac gtatgcagcg aggtcaacgc gtgcgtcgat caaccggttc
     3061 agcagatcga ttcgctgtct gtcaacgaac atacttcgat cctcctcttt ccagccttgt
     3121 tgcaattaaa tagtatggct attttttgag caaaatgctg tccaggatca attcttcacg
     3181 ccgaattaac aaaatttaca tttccttcgc ctccgccagc gaatgagatt gcgatacaaa
     3241 ttttatccag aactctgtag aatccctgcc ctgacctttc ctgtaactga atatttttta
     3301 agctatgagc aaagtaactc cccagccgaa aatcggcttt gtttcccttg gctgtccgaa
     3361 aaaccttgtc gattcagagc gtattctcac cgaactccgc actgaaggtt atgacgtggt
     3421 accgagctat gacgatgcga acatggtgat cgtcaacacc tgcggcttta ttgacagcgc
     3481 ggtacaagaa tcactggaag ccattggtga agcgttgaat gaaaacggca aggtaattgt
     3541 gaccggttgt ctgggggcaa aagaagatca gatccgcgaa gtccacccga aagtgctgga
     3601 aatcaccggg cctcatagct atgagcaggt tctggagcac gttcatcact acgtgccaaa
     3661 accgaaacac aacccattcc tgagcctagt gccagaacaa ggtgtgaaac tgacgccgcg
     3721 tcattatgcc tatctgaaaa tttcggaagg ctgtaatcac cgctgcacct tctgcattat
     3781 tccgtctatg cgcggcgacc tggtgagccg tccgattggc gaagtgttaa gtgaagcgaa
     3841 acgtctggta gatgcgggcg ttaaagagat tctggtgatc tcgcaggata cttccgccta
     3901 tggcgttgat gttaaacatc gtactggctt ccacaacggc gagccggtaa aaaccagcat
     3961 ggtcagcctg tgcgaacagt tatcgaaact ggggatctgg acacgtctgc actacgttta
     4021 cccttatccg catgtggacg acgtcatccc actgatggca gaaggcaaaa tcctgccgta
     4081 tctggacatt ccgttgcagc acgccagccc gcgcattctc aaactgatga agcgtccggg
     4141 ttctgtagat cgccaactgg cgcgcatcaa acagtggcgc gaaatctgcc cggaactgac
     4201 cctacgctca acctttattg tcggcttccc tggcgagacg gaagaagatt tccagatgct
     4261 actcgacttc ctgaaagaag cacgtctgga tcgcgttggc tgctttaaat acagcccggt
     4321 tgaaggtgca gacgccaatg ccctgcctga ccaggttccg gaagaagtga aagaagaacg
     4381 ctggaaccgt ttcatgcagc tgcagcagca gatttccgcc gagcgcctgc aagagaaagt
     4441 gggccgtgaa attctggtga ttatcgacga agtggacgaa gaaggcgcga ttggtcgcag
     4501 catggcagat gcaccggaaa tcgacggcgc ggtttatctc aatggtgaaa ccaacgttaa
     4561 gccgggtgat atcctgcgtg tgaaagtcga gcacgccgat gagtacgatt tgtggggtag
     4621 ccgggtttaa ttaattcagg ccgggcaaaa tcatttgtcc ggcctgcgaa taatgattac
     4681 tgccagctaa tctgtgccct gccgtttttc ttactcttat acagccgctc atccgccagc
     4741 attttcaagc gttcgacggt agtacattca tttgaatgcg ctacaccgat actggtgctt
     4801 aaattaatgc ttcccacctg gaaatgaacg atttctttta gtatcgatag caactcttcc
     4861 agcgtgccgc cagtataaat caccgcaaat tcgtcacctc cgaagcggta gactttacct
     4921 ttatctaccg ggatctgtga tttaaggatt ttcaccactt tcatcaacac ttcatcgccc
     4981 agcaaatgac cgaaggtgtc gttaatgttt ttgaatttat cgacgtcaaa cagacacaaa
     5041 taaccgcctt tttccggcag cgcttttaaa tcttcatcaa aggattttct gcccattgct
     5101 ttggtcaggt cgtccaggaa cgccgagtta tacatactgt agagtttttt gacatcggca
     5161 gccgaaaaga aaagcccgcc atccagtacg cgttcaaaat ggttattaat gatggcttct
     5221 ttctccacaa taccacgatt aatcactctg cgtaccagat agaggcaact ggcacccaat
     5281 atcactgcca ccaatgcggc aaagaaaata atcaatgtac aaacccgcaa ataaacatct
     5341 tttttatacg caaatacgta caatccggtc ggcaaatagg cgaagtgctc catcgcttcc
     5401 gaaacggtga agatggcgtt tttagcatta tattcatcac cggtcaccgg tttaccgttc
     5461 ttaaatagag agaacatcag gttctcgctg tccatcacca gcgattttga cagtaaagaa
     5521 aaacgctcca gaccaagcaa aaatatcaga ctacgattgt gagttaccgg atagaggtaa
     5581 tagacgaagg ttttaccgtc aaaagagata aaccctgtat ttttgacatg tcgatacttc
     5641 acaacctgca taatatctgc acaataattt ttctcgaact cctttacctg aatacattcg
     5701 tcactggaaa tcagataagg accgacatcg tacaaataag agggggaaac cgagtcggcg
     5761 taagctgtgc gcaaaccaac caacagctgt tcctccgtgg gatttagcgt gttgataaga
     5821 tcaattgcca cgctacgccg atattcagca atatttgcca ccgcaaaagc agtatcaata
     5881 acggactgtt tcaggctata cacccgttcc tttaccattt gagtgtagat cccgcaggca
     5941 actgctgaaa tcatgataaa aatcagcaga ctgactgtaa gtacgaactt attgattctg
     6001 gacatacgta aactactctt ttactaattt tccactttta tcccaagcgg agaatggcat
     6061 gggtttctgg tacagatacc cctgagcctg aacacatcct aatttaatta attcgcgcgc
     6121 aacgtcttcg ctctctacgc cttccgcaat caccgggatc cggttatagc gtgccagatc
     6181 gaaaataatg cgcacaattt ttcccgcagt accgaaaata tcgccggtaa attgcgagtc
     6241 cacctttatg ctgtctggat taatttcata aaaacgtttc aggtttgaca aaccggtgcc
     6301 aaagtcatca atcgatatcc gtaccccttg tttacgcagt tgttcaatat tgagataaaa
     6361 atgttcatta attcgctcga tcacggtttc agtgatctca acacagaggc tgttcgcctt
     6421 caggccattt tctttcatga tggtggtaaa acgctcatga aagtgcggtt cgtttaactg
     6481 ccagggcgaa acattaatat gcagtaagaa atcatccttc agcgacgcac gttgactaaa
     6541 atgtctgaat tcacccattg ccgttttaat aatctgataa cccagctcat tgataaaccc
     6601 gatatcttca gcaatggtaa tgaacttcag cggtgaaatg atcccccgct ccgcagaaac
     6661 ccaacggcac aacgcctccg cgctcagaat ggcgcgggtg ttaatatcaa cgatcggttg
     6721 atagacgagg aatagttcgc caatttccat tgcatggcga atattacgcg caatccagat
     6781 attctcttct atttcgttga gtttcgcgtc atcacagaaa ataaactcgg agaacatatg
     6841 gtcacggata ctggaaatcg ccagtttcag tttttctgcg taactccagg cgttttcccc
     6901 ctgcaaaggt tcacaaatgg cagacttatg gatgcgcaga ttgcggtagc caaagctgct
     6961 gtcaaaatcg tttaccatcg ctattttacg ggtaaatgtc tgtacatttt cccgacacag
     7021 aagtaaatag agatcggcat tgaatgtcac gatatcgcag cattgcgcat atttctcggc
     7081 aattcgctga cacaatttcc tgacgatttt attgcgttca tcaacaccaa acaccgcctc
     7141 cacggcttcc agaccacaca gtttgatgat ccccacagcc agatactgat tattgttgga
     7201 ctcaataatc gccatatttt taataaaacc gctttcctga tcgtaagaga tcagtgagag
     7261 caatttattt gaccgttcaa tttcggcatc tttcatttcc gcgatcgaat ttttgatgct
     7321 gataatctct ttcgcatcgc cttcgcgaat tttcagagcc tcgatctgat ccgttgagtc
     7381 gggcaaaaac gccagcgtat taaccagctc ggtcatttca gaaaattgcc gacggaacac
     7441 acgagtggtc atgaaatata ccaccagcaa cacgataata atgaacgcaa acggtagata
     7501 aaaaagttta tctgatgcgt tcaataaatt accgccagga taataaaaat agatttcgaa
     7561 caatgcatta tttggcactg ctgatgtctt tgcaatatac tccgtatcga cagaatcaat
     7621 aatattataa ttaccttcgc tattataaat aatcagtttt tcacgagcga gttgattctt
     7681 ggcaatgatc tcatgacctt gtttcaaaaa tagaacttca tcattagcgc tgatttgatc
     7741 aagatactcg ttcagatacc gccagtttaa atcgacatga attttatcta ccgctaacaa
     7801 atcgtgatct ttgccgttga tacgtagcgt aaaagagata cattttttac ccggacgatc
     7861 aaagcaattg tatgccggat tccagctgtt gttagagtca tctgaagcca ccgaccacgg
     7921 tcgtttcgat gggttaaaat cggcaaaaat aacccgccct gactcgtcga caagcggtcc
     7981 atttacttgt tcctgatgat aaacaacgat tttatcattc gccctccgcg aaaagagata
     8041 atatttatta tcatcaagta ccagtcccac cgaatctaaa aatggcatca tttccatttt
     8101 cagaatcacg gcgcgttgaa tctcctctgt attttttaat aaatacagtg agtcctttaa
     8161 tgtcagggcc gaacgttcaa tatctttaaa gaaatagcca acgtttttct gaataagttc
     8221 aaagcggcta tcggcacgtt ttaccgcgcg ttccgatacc agttgatagt taatcatgaa
     8281 aaaaagacca ataaatgaca gtgctgccaa taaaaataaa attatcagcc ttatctttat
     8341 cttttcgtat aaactcagca tgttaataca tttaacaaaa taactatctg aacgctgtgc
     8401 cgctgcaaaa atagtccctt gctgcggctt tttaaccaaa ttacccgatt ctacaaaatc
     8461 agcagaaagt tacaacactt gcttagggca ataatttcct gtgaggtgat taccctttca
     8521 agcaatattc aaacgtaact atcctttaat tttcggatcc agcgcatcgc gtaaaccatc
     8581 gcccaacaaa ttgaacgcca gtacggtcag aaaaatagcc agggccggaa aaacagcgac
     8641 atgcggcgcg ataaccatat ccgctcgtgc ctcattgagc attgctcccc actctggtgt
     8701 cggcggctgc gcaccgaggc cgagaaatga caggctggcg gcagagataa tcgaggtgcc
     8761 aatgcgcatg gtgaaaaaca ccacgataga agagacggtc ccaggcagga tatgacgcaa
     8821 taaaatggtc atatcgctgg caccaatact gcgtgctgac tcaataaagg tttgctgttt
     8881 caacaccagc gtgttgccgc gcaccaggcg ggcaaacgcg gggatggaaa aaatggcgac
     8941 tgcgataatc acgttagcaa tgccgcttcc caacaccgca acaacagcga tcgccagtaa
     9001 aatacccggg aaggcaaaca gcacatcgca aatgcgcatg atcagccgat cccaccagcc
     9061 ttcataatat ccagccaaca agcccagcaa cgtccctatc gccacaccga taaacacggc
     9121 aaacacgccc gccgccagcg agatttgcgc accaaccagg acacggctga aaatgtcgcg
     9181 ccccagtgaa tcgacgccaa accagtgctg caaagaaggt ccgttattca gattgtcata
     9241 atcaaaataa ttttcggcgt catagggagc gatccagcgt gcaaaaatgg ccaccacaat
     9301 caataaaata acgaataatg cggcggtcat cgccatatgc tgacggcgaa atcgtcgcca
     9361 gaattcatgc cacggtgtac gtacctggtc aggtttgacc agtggcatgg cgtttaacac
     9421 cgcctgacgt cgccagttaa atagtcgcat ccttacttgt acctgatagc cgggttaatg
     9481 gcggcgtaaa gcacatccac cactaagttg ataagaataa attccagcga gaaaagcaga
     9541 atttccgcct gaatcaccgg gtaatcgcgc atttctacag agtcaacgag taagcgtcca
     9601 agtccgggcc agttgaaaac tttctccacc acgatagaac caccgagcaa aaagccaaac
     9661 tgtaagccca tcatggtcac taccgggatc atcgcgttgc gtagcccgtg tttgaggaca
     9721 acccaggttt cgctcacccc tttcgccctc gcggtacgca tataatcttc gcttaaaacg
     9781 tcgacaaatg acgcacgggt aaagcgcgcc atcacggcgg ccactgccgc gccgagcgtc
     9841 agggagggta aaatgtagtg ctgccaactg tctgctccca cagtaggcag ccagcccagt
     9901 tcaacggaga atacctgaat taaaaacatc cccagcgcaa atgccggaaa cgagatcccc
     9961 gacaccgcaa tggtcatact caatcgatcc ggccaacgat tacgccagac ggcggcgata
    10021 attcccgccg ccataccaaa tatcaccgcc cagaccatac tggttatggt cagccacagc
    10081 gttggcataa agcggctggc aatctcatcg gcaaccggac gacgcgacac catcgacagg
    10141 ccaaaatccc cctgcacagc attgctgata tagtgccaga actggtgata cagcggctga
    10201 tccaacccca gctgctgacg caccagttct ataacctgcg catcagcttc gggcccggca
    10261 atcaatcgcg ccggatcgcc gggcagcatg tggacaaata aaaacaccag caccgagacg
    10321 ataaacagcg tcggaatcaa ccccagtaat cgtttgataa cgtaattaag cattccactc
    10381 cctgcgttgc ttattgcaaa tccgcgtctt caaagctgaa gccggtatct ggcatgatcc
    10441 aaaaaccggt caggttttta ctgtgtgccg acaccagttt ttctaccacc agcgggatcc
    10501 acggcgattc ttgccagatg atatcctgcg ccgccttata taagcgggtc ttttccgccg
    10561 gatcattcgt tttcagtgcc tgagccagga agtcatccac ctgtttattg ctgtaaaacg
    10621 cggtattaaa cagcgtcggt ggccagtttt gcgaggcaaa cagcggcgat agtgcccagt
    10681 cggcttcgcc ggttgaagcc gaccagccag tgtagaacat ccgcacgcca ctctcttttt
    10741 gccctttacc ttcaacttct gccgcccgct gtccggcatc catcgcggtc acctgggctt
    10801 taatcccgac ctgcgctaac tgctgctggg taaattgcag cactttctgc gcggtgctgt
    10861 ggttatgtga cgaccacagc gtggtactga aaccgttggg atatcccgcc tctttcagta
    10921 attcgcgcgc tttcactgga tcgtaaggcc acggtttata actttgcgcg taggcgatac
    10981 ttggcggtac cacaccagta gctggcgttg catagcccgc aaaggcaact ttcaccagcg
    11041 ccggacggtt aatggcgtaa ttcagcgcct cacggacctt cgggttatcg aacggctttt
    11101 gcgtcacgtt catactgata taacgctgca taattgacgg actggccatc aactcgatat
    11161 ttttgttttt ctccagcagt gtggcctgct cgtagggaat ggggaaagca aactgcgctt
    11221 caccggtttg cagcattgcc gcgcgggtgt tgttatccgc caccggacgc caggttatgc
    11281 tgtccagttt gggcaatcct ggctgccagt aacccgcgaa ttttttcacc ttcacaaaat
    11341 cggtctgatt ccaggtatcc agttcatacg gtccggttcc caccggatga aaaccaatct
    11401 ccttgccata tttttccagc gctgtcggtg aaatcatcgc ggtcgccgga tgggcaagaa
    11461 tattaataaa cgctgagaac ggctgtttga gggtaatctt taccgttgtc ggatcgatcg
    11521 cctccgtttt ggcaatattc ttatacaggt tatagcgttt aagatgattc gccggatcgc
    11581 tggcccggtc cagattcgct tttaccgcca ctgcgttgaa atcggtgcca tcctggaatt
    11641 taattccttc ccgcaatttc acggtgtaag taatgccgtc atcggaaacg gtataactct
    11701 ccgccagcac gtttttcagt ttcatctctt tatccagacc gaacagcccc tggtaaaacg
    11761 atttcgctac ggcctgagat aacgtgtcat tcgcgtcata cggatcgagc gtggtgaaat
    11821 tcgatcctac cgccaccacc acatctttgg cagcgaatgc acaagatgcc atcaacgctg
    11881 tcgcaatgcc cagcgccact aacccactac ggtgtacagc tcttgccatt ttattctccg
    11941 cctgaatgtt atctacgcat gaatgcgtat tctgattgtg gttgtgcgac gtaatgcccc
    12001 ggcccgacgc attgcaacga gacggctgcc acctcttcgc cacgcagatg aatattgctg
    12061 ggaagatcgt ccgacagcag tacacgctgc ggtcgttgtc gggacggttc agcgaccgga
    12121 actgccgcca gtaatttacg cgtataagga tgctgcgggt tttcgaagac cgcgcaccgt
    12181 gaaccaattt caacaatttg cccgagatac atcaccgcca cacgatgact aatccgctct
    12241 accacagcca tatcgtggga gataaacaga tacgcaatgc cgaaatcacg ctggagatcg
    12301 agcaacaagt tgataatctg cccgcgaata gaaacatcca gcgccgaaac ggcttcgtcg
    12361 gcaatgatca cttttggatt caatgccaac gcgcgagcaa tgcagatgcg ctggcgctga
    12421 ccgccggaaa actcatgcgg gtaacgccag gcatgttcag gtaacaggcc cacgcgctcc
    12481 agcaaccacg caacgcgtgc agccgcgtct ttacctggca ataaaccgtg tacacgcagc
    12541 ggttcgataa tcgaatcacc gatggtctga cgtgggtcca gcgaagcgta agggtcctga
    12601 aaaataaact gaatatcccg gcgcaatgcc tgaagtttgc caggtgacaa ggtatcgatt
    12661 cgctgaccgt taaagataat ttcgccgccc tgcgattcga ccaggcgcag caacgcccgc
    12721 ccggtagtgg atttaccgct gccagactcg cccaccagcg atagcgtttc gccaggccag
    12781 agatcaaaac tgactttctc aacggcatgc acttcccgcg ttacgcgatt caacaaaccg
    12841 ctgcgcaaag ggaaacgggt gacaagatta cgcacccgta aaacaggttc gccatccacc
    12901 accgttttct gctcgatggg gggttcctgt ttcgctggat gttcaagcga tatcaatggg
    12961 aaacgtcggg gataatctaa ccctttcatc gcaccaagtt gcggaacagc agctaacagc
    13021 gcacgggtgt aaggatgttg cggtgcatga aaaatctgtt cgacggtacc cgtttccacc
    13081 gcctcgccct gatacatcac cagtacccga tcggcaatct ctgccaccac gcccatatcg
    13141 tgagtgataa agataacgcc catcgacatc tctttttgca atactttgat taattgcagg
    13201 atctgcgcct gaatagtgac atccagcgcg gtggttggct catcggcaat cagcaccgcc
    13261 gggcggcatg acagcgccat cgcaatcatc actcgctggc gcatcccgcc agagagttga
    13321 tgcgaataac gtgaaagaat ggtttgtgcc tccggaatgc gtacctgatc cagcatccgc
    13381 ttcgcctcga ccatcgcttc ttcacgactg gcgttctgat gcagacgaat tgattcggca
    13441 atctgttcac ccacagtaaa tactgggttc agcgatgtca tcggctcctg aaatatcatc
    13501 gccatatccg caccgcgcac atggcgcatt tgtgcagcgc tctgctcgct aagttcaatc
    13561 acatcacgac tgcgccgccg caacagcatt ttatcgcact gtactaaacc gcccgcctgt
    13621 tccaacagac gcatcaatgc cagcgcagtc actgacttac cggagccgga ttcgcctaca
    13681 attgccagcg tctcaccgcg ttgcagacta aaagagagat tgcggaccgc agctattttc
    13741 tgctggtcct gcataaaggc aatattaaga ttttcaaccg ccagcacatt accggcatca
    13801 agttcatcac tgtgtggcaa cggtgtcccc tttttcacgg tagataccgg tggttggcgt
    13861 atcgcctgcg tagccccagg cgcgatacat tccttcggtg ttaaacggta gcgcgacatt
    13921 cccttcatgg tcgatagcga ttaagccacc gctaccgcca agcgcaggga gtttttccat
    13981 tactacccgc tcgcaggctt ccgcgagact taatccgccg taatccatta acgcggcgat
    14041 gtcatatgcc gccagcgcgc ggatgaagac ttcgcctgtg ccggtacaag aaaccgccac
    14101 actggcgtta ttggcgtagc atccggcacc cactaaggga ctatcgccaa ctcgtccggg
    14161 taatttattg gtcattccgc ctgtggacgt ggctgccgcc agattgccgt ctaaatccaa
    14221 cgccacggcc cccacggtgc ccattttctg tttttcatcc agtggcgcac cgctatggtc
    14281 gaggactgtt gccccttcct cgcgcgctgc cagtagttgt tcataacgca aaggcgtgga
    14341 gaaaatctcc ggtgagacgc gctccatgcc atgagcaaac gcaaaatttt ctgccccttc
    14401 gccaatcatc atcacatgcg ggctttgctc catcaccagc cgggcggcaa gaaccggatt
    14461 acgcagatga ctaacgcccg ccaccgcacc ggctttcagg gtgttaccat ccatcacaca
    14521 ggcgtccagt tcatgggttt catcacgcgt aaagacagcg ccaattccgg cgttaaacag
    14581 tggacactct tccagcagac gcaccgcttc cgtcaccaca tccagcgcac tttcgccggc
    14641 ttccagcatt ttctgcccgg tttcaacaat ggcagacaac gcctcgatgt agcgtaattc
    14701 ctgttgcaga ctcatctgcg cgcggctaat tgcacctgcg ccaccatgaa ttgcaatgac
    14761 tgctttgccc atagtattcg tccattaagg gtggctcgcc acttttgcta taaatatcag
    14821 aatttttatg aattactcat tcaatttttg aatatagaaa gatggatatg gcatgtaaag
    14881 gcaggagcca acgctacgcc cgaggtggca tacagtctat ggcgtttttc tgacataata
    14941 ggcaaattcg attttgcctc cgcaggagtg ttttcatgga atttaccacc ggattgatgt
    15001 cgctcgacac cgcgcttaat gagatgcttt ctcgcgttac cccactgacc acccaggaaa
    15061 cgctgccact ggttcagtgt tttggtcgta ttctggcgag cgatgtcgtt tcgccactgg
    15121 atgtcccggg gtttgataac tccgctatgg acggctacgc ggtgcgttta gccgatattg
    15181 cctccgggca accgctgccc gttgccggta aatcctttgc cggtcagcca taccatggtg
    15241 aatggcctgc gggtacctgc attcgtatta tgaccggtgc gccggtgccg gaaggctgcg
    15301 aagcggtggt gatgcaggag cagactgaac aaacggacaa tggcgtgcgt tttactgctg
    15361 aagtgcgtag cgggcaaaat attcgccgtc gcggtgaaga tatctctgca ggtgcggttg
    15421 ttttcccggc gggaactcgc ctgactaccg cagagctgcc agtaattgct tcactgggga
    15481 ttgccgaagt tccggtgatt cgtaaagtgc gtgtagcgct tttttctacc ggtgatgaac
    15541 tccagttgcc cggtcagccg ctgggcgacg gccaaatcta cgacaccaac cgtctcgccg
    15601 tacacctgat gttagaacag ctgggttgcg aggtaatcaa cttagggatt atccgcgacg
    15661 atccacatgc cctgcgcgcc gcatttattg aagccgacag ccaggcggat gtagtgatca
    15721 gttccggcgg tgtttcagtg ggtgaagcgg attacaccaa aatgattctt gaagagctgg
    15781 gggagatcgc cttctggaag ctggcgatta aaccaggtaa accgttcgcg ttcggtaaac
    15841 tcagcaatag ctggttctgc ggcctgccgg gcaacccggt ttcagcgacg ctgaccttct
    15901 atcaactggt acagcctttg ctggcaaaac taagcggcaa caccgccagc ggcctgcccg
    15961 cgcgccagcg cgtacgcaca gcgtcccgcc tgaaaaaaac gccaggacgt cttgatttcc
    16021 agcgcggcgt gctgcaacgc aacgccgacg gtgaacttga agtgacgacc accggacatc
    16081 agggttcaca tatatttagc tcctttagtc tcggcaactg ctttatcgtg ctggaacgcg
    16141 atcgcggcaa tgtggacgtg ggcgaatggg tggaagtaga accgtttaac gcgttgttcg
    16201 gaggcctgta atggcggaac tcagcgatca ggagatgctg cgctacaacc ggcaaatcat
    16261 tctgcgcggt tttgattttg acggtcagga ggcgctaaaa gattctcgtg tgctggtagt
    16321 cggcctcggt ggactcggct gcgcagcttc gcagtatctg gcaagcgccg gtgtcggcaa
    16381 cctgacgctg ctcgacttcg acacggtttc gctctcgaat ctgcaacgcc agacactgca
    16441 cagtgatgcc acggtcgggc aaccgaaggt ggaatccgcc cgtgacgccc tgacgcggat
    16501 caacccacat atcgcgatta cgccagtcaa tgcactgcta gatgacgcag aacttgcagc
    16561 attgattgct gaacacgatc tggtgctcga ctgtacggat aacgttgcgg tacgtaatca
    16621 actgaacgca ggctgttttg ccgcgaaggt gccgctggtt tccggcgcgg caattcgtat
    16681 ggaagggcaa atcacggtct tcacttatca ggacggcgaa ccgtgctatc gctgccttag
    16741 ccgtttgttt ggcgagaacg cgttaacctg cgtagaagca ggcgtgatgg caccgttgat
    16801 aggcgtaatt ggttcgttgc aggcaatgga agcgatcaaa ctgctggcag gttatggcaa
    16861 accggccagc gggaaaatcg tcatgtacga tgcgatgacc tgtcagtttc gcgaaatgaa
    16921 actgatgcgc aatccggggt gtgaggtgtg cgggcagtaa ttgccacctc caagcctgat
    16981 aagacgcgac aagcgtcgca tcaggcattt gctccagacg ccagatgcgg cgtgaacgcc
    17041 ttatctggcc tacggttcgg gcacagattt ccaagcgcgg caggcatcgg tgcactccgc
    17101 cacgtcatca gcaatttatg aggatgcaga acgtccggtt aaatcgacgt tctgccaaac
    17161 gctccctgcc agtcctgctc aaacttcgtc acagcggctt ccaccgccgg atagctaatc
    17221 atctgttgtg ccacatccag tggcagagta attgattcac atccggccag taagcagtcc
    17281 agcgcctgac gcggggtttt gaaactcgct gccagcactt tcgcctgcgg cgcatgcatt
    17341 ttcaataact ggtgtaagtc ggtcacagtc tgaatgccgc taccgccctg agcatcaata
    17401 cgattaacgt aaggcgcaac atattccgca cctgccagcg ccgacagcag cccttgtgcc
    17461 gcgccatata ccgcggttcc cagcgtcgga atcccttccg cttttaacat cttaatcgct
    17521 gccagcccct cggctgtcac cggaactttc accacgatat ccgcaataat agaacgcagc
    17581 ttaagcgcgt cattcaccat cccttcagca gtggtagcca ttacctgggc aaacagacgc
    17641 ccctgaccgc ccatcgcttc atgaagttgc ggaagcacaa catccagcgg ttttttaccc
    17701 gcggcgataa tgcttgggtt agtggtcaca cccgccagcg gaaaaatacg tgacagcgcc
    17761 ttcaccgcaa caacgtctga agtatccaga tacagttcca taaccatcct caaaatgtac
    17821 tttttgtctg aatgtagact atcacgggaa aactctctga acagttgacg cacatcaatc
    17881 taactttcat tcgaaagtat tttaatgttt gaacgaaaca gagaggcaac tatgattttc
    17941 aatattcagc gctactcgac ccatgatggc cccggtatcc gcacggtcgt atttcttaaa
    18001 ggctgttcgc tgggctgccg ctggtgtcag aacccggaaa gccgcgcccg tacgcaggat
    18061 ctgctgtatg acgcacgact gtgtctggaa ggctgcgagc tgtgcgctaa ggccgcgccg
    18121 gaagtgattg agcgcgcgct gaatggtttg cttattcatc gggaaaagtt aaccccggag
    18181 catctgacgg cgttaaccga ctgctgtccg acacaggcgt taaccgtgtg tggtgaagtg
    18241 aaaagcgttg aggagatcat ggcgaccgtt ctgcgcgata aaccgtttta cgatcgcagc
    18301 ggcggcggct taacgctttc gggtggtgaa cccttcatgc agccggaaat ggcgacggcg
    18361 ctgctgcaag cctgccacga ggcaggcatt catactgcgg tagaaacctg tctgcatgtg
    18421 ccgtggaaat atatcgcccc ttctctgccc tatatcgatc tgtttcttgc cgatttaaaa
    18481 cacgttgccg acgcgccgtt taaacagtgg accgacggta acgccgccag ggtactggat
    18541 aacctgaaaa aactcgccgc agcgggcaaa aaaatcatta tccgcgtgcc gctgattcag
    18601 ggctttaatg ccgacgaaac ctctgtaaaa gccattaccg attttgccgc cgacgagctg
    18661 cacgttggcg aaattcattt tctgccctac cacacgctgg gcatcaacaa atatcactta
    18721 cttaatctgc cctatgacgc cccggaaaaa ccgcttgatg cgccagagct gctcgatttt
    18781 gcccagcagt atgcctgcca gaaagggtta accgcgacct tacgaggata acaaccatga
    18841 ccacactgaa actggacacg ctcagcgacc gcattaaagc gcacaaaaat gcgctggtgc
    18901 atattgtgaa gccgccagtc tgtaccgagc gcgcgcagca ctataccgag atgtatcaac
    18961 aacatctcga taagccgatc ccggtacgtc gcgcgctggc actggcgcat cacctggcga
    19021 atcgcaccat ctggatcaaa cacgatgagt tgatcattgg caaccaggca agcgaagttc
    19081 gcgccgcgcc gatcttcccg gaatatactg tctcgtggat cgaaaaagag attgatgatc
    19141 tggcagatcg tcccggtgct ggctttgcgg tgagcgaaga gaacaaacgt gttctgcatg
    19201 aagtgtgccc gtggtggcgc ggtcagacgg tacaggatcg ctgctatggc atgtttaccg
    19261 atgagcaaaa aggtctgctg gcgaccggca tcattaaagc ggaaggcaat atgacctccg
    19321 gcgatgcgca cctggcggtc aatttcccgc tgctgctgga aaaagggctt gatggtctgc
    19381 gtgagaaagt agcggaacgt cgctcgcgca tcaatctgac ggtgctggaa gatttgcacg
    19441 gcgaacagtt cctgaaagcg attgatatcg tgctggtggc agtcagtgaa cacattgaac
    19501 gtttcgctgc cctggcgcgt gcaatggccg cgaccgaaac ccgcgaaagc cgtcgcgatg
    19561 aactgctggc gatggcagaa aactgcgatc ttatcgccca ccagccgccg cagactttct
    19621 ggcaggcgct gcaactgtgt tacttcatcc agttgatttt gcagattgaa tctaacggtc
    19681 actcggtgtc gtttggtcgt atggatcagt atctctaccc gtactatcgc cgcgacgttg
    19741 aactgaacca gacgctggat cgcgaacacg ccatcgaatt gctgcatagc tgctggttga
    19801 aattgctgga agtgaacaaa atccgctccg gctcccactc aaaagcctct gcgggaagtc
    19861 cgctgtatca gaacgtcacc attggcggac aaaatctggt tgatggtcaa ccaatggacg
    19921 cggtgaatcc actctcttac gcgatcctcg aatcctgcgg tcgcctgcgt tccactcagc
    19981 ctaacctcag cgtgcgttac catgcaggaa tgagtaacga tttcctcgac gcctgcgtac
    20041 atgtgatccg ctgcggcttc gggatgccgg cattcaacaa cgacgaaatc gtgatcccgg
    20101 aatttattaa actcggtatt gaaccgcagg acgcttatga ctacgcagcg attggttgta
    20161 tagaaaccgc cgtcggtggc aaatggggct atcgctgtac cggcatgagc tttatcaact
    20221 tcgcccgcgt gatgctggcg gcgctggaag gcgggcgtga tgccaccagc ggcaaagtgt
    20281 tcctgccaca agaaaaagcg ttgtcggcag gtaacttcaa caacttcgat gaagtgatgg
    20341 acgcgtggga tacgcaaatc cgttactaca cgcgtaaatc aatcgaaatc gaatatgtcg
    20401 tcgacaccat gctggaagag aacgtgcacg atattctctg ctcggcgctg gtggatgact
    20461 gtattgagcg agcgaaaagt atcaagcaag gcggcgcgaa atatgactgg gtttctggcc
    20521 tgcaggtcgg tattgccaac ctcggcaaca gcctggcggc agtgaagaaa ctggtgtttg
    20581 aacagggcgc gattggtcag caacagctgg ctgccgcact ggcggatgac ttcgatggcc
    20641 tgactcatga gcaactgcgt cagcggctga ttaacggtgc gccgaagtac ggcaacgacg
    20701 atgataccgt cgatacgctg ctggctcgcg cttatcagac ctatatcgac gaactgaaac
    20761 agtaccataa cccacgctac ggtcgtggtc cggttggcgg caactattac gcgggtacgt
    20821 cgtctatctc cgctaacgta ccgtttggcg cgcaaactat ggcaacgccg gacgggcgta
    20881 aagcccacac cccgctggca gaaggcgcta gtccggcctc tggtactgac catctcggcc
    20941 ctactgcggt cattggctca gtgggtaaac tgcctacggc agcgattctc ggcggcgtgt
    21001 tgctcaacca gaaactgaat ccggcaacgc tggagaacga atctgacaag cagaaactga
    21061 tgatcctgct gcgtaccttc tttgaagtgc ataaaggctg gcatattcag tacaacatcg
    21121 tttcccgcga aacgctgctg gaggcgaaaa aacatccgga tcagtaccgc gatctggtag
    21181 tgcgtgtcgc gggttattcc gccttcttca ccgcgctctc tccagacgct caggacgata
    21241 tcatcgcccg tactgaacat atgctgtaat ctcttcggcc cggctgcggc gtcgccgggc
    21301 taaatcattc acatcatcaa tttcatcctt actttcattc gaaatataat ttgtgctctg
    21361 cgtcacattg ttattacact gttacggttt cattttcgag ccaggagcac acctatgagc
    21421 gtaaaagtta tcgtcacaga tatggacggt acttttctta acgacgccaa aacgtacaac
    21481 caaccacgtt ttatggcgca atatcaggaa ctgaaaaagc gcggcattaa gttcgttgtc
    21541 gccagcggta atcagtatta ccagcttatt tcgttctttc ctgagctaaa ggatgagatc
    21601 tcttttgtcg cggaaaacgg cgcactggtt tacgaacatg gcaagcaact gttccacggc
    21661 gaactgaccc gacatgaatc gcggattgtt attggcgagt tgctaaaaga taagcaactc
    21721 aattttgtcg cctgcggtct gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattt
    21781 gtcgcactga tggcaaaaca ctaccatcgc ctgaaacctg taaaagatta tcaggagatt
    21841 gacgacgtac tgttcaagtt ttcgctcaac ctgccggatg aacaaatccc gttagtgatc
    21901 gacaaactac acatagcgct ggatggcatt atgaaacccg tcaccagtgg ttttggcttt
    21961 atcgacctga ttattcccgg tctacataaa gcaaacggta tttcgcggtt actgaaacgc
    22021 tgggatctgt caccgcaaaa tgtggtagcg attggcgaca gcggtaacga tgcggagatg
    22081 ctgaaaatgg cgcgttattc ctttgcgatg ggcaatgctg cggaaaacat taaacaaatc
    22141 gcccgttacg ctaccgatga taataatcat gaaggcgcgc tgaatgtgat tcaggctgtg
    22201 ctggataaca catccccttt taacagctga cctctctacc ggagccatgc gctccggttt
    22261 tcccttcttt ttacatcctg tacatcacat caaatcatta aacttgcatt aacttattta
    22321 taacttgtat tgtcacatgc aaattagcat agtttcgaat gaaagttaat gcgaggtggt
    22381 tatggcttct acttttacca gcgacacatt gcctgccgat cacaaagcag ctatccgtca
    22441 gatgaagcac gcgctgcggg cgcagcttgg cgatgtccag cagatcttta atcagctaag
    22501 cgatgacatt gccacgcgag tggctgaaat caacgcactc aaagcacagg gcgatgccgt
    22561 ctggccggtg ctgtcttatg ccgatatcaa agcaggtcat gttactgcag agcagcgcga
    22621 acagattaaa cgtcgcggtt gtgcggtgat aaaaggccat ttcccccgcg aacaagcgct
    22681 aggctgggat cagtcgatgc tggactatct gaaccgcaac cgctttgacg aggtctacaa
    22741 aggccccggc gataatttct tcgggacgct cagcgcttca cgtcccgaga tttaccccat
    22801 ctactggtcg caggcgcaaa tgcaggcccg ccagagtgaa gaaatggcga atgcgcagtc
    22861 gtttctcaat cgtctgtgga catttgaaag tgatggaaag caatggttta acccggatgt
    22921 gagcgtcatc taccctgacc gtatccgccg ccttccgccc ggaacgacct ccaaaggtct
    22981 tggagcgcat accgactccg gggcgctgga acgctggctg cttccagcgt atcagcacgt
    23041 tttcgccaac gtctttaatg gcaatctggc gaaatacgat ccctggcatg cggcacatcg
    23101 tacggaagtt gaagagtaca cggtggacaa caccaccaaa tgttccgtgt ttcggacatt
    23161 ccagggctgg actgcgctct cggatatgct gcctggtcag gggttgctgc acgtcgtgcc
    23221 cattcctgaa gccatggcgt acgtactgtt acgtccgctg cttgatgatg tgccggagga
    23281 tgaactgtgc ggcgtagcgc ccggaagagt gttgccggta tcagagcaat ggcatccact
    23341 gttaattgag gcgttaacca gcattccaaa actcgaggcc ggagactccg tctggtggca
    23401 ctgcgacgtc atccattccg ttgcccccgt tgaaaatcaa cagggttggg gcaacgtgat
    23461 gtacattcct gcggcaccga tgtgcgagaa aaatcttgcc tacgcgcaca aggtgaaggc
    23521 cgcactggaa aaaggcgcat cgccgggcga cttcccgcgc gaggactatg aaacaaactg
    23581 ggaaggacgc tttacgcttg ccgacctcaa cattcacggt aagcgagcgt tgggcatgga
    23641 tgtttgagag ttaatggaag tgaattgatg tgggattaag aaaatcgatt cgacgttact
    23701 cagattgctg acaagtgcgc tttcttaatg cctgatgcgg cgttaacgcc ttatccggcc
    23761 tacaaagtcc tgcagattca atatattaca ggtactgcgt aggcctgata aacgtagcgc
    23821 atcaggctat tttgcatttg tcatcagccg tcgtcacccc gacagtatcg gggtgacgga
    23881 taatcactta ctcgatccct ttactacgca ggtaatcttc gtaattaccg ctaaagtcga
    23941 tcacgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg aactcacggt
    24001 cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc agtgactcga
    24061 tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc ggcttctgca
    24121 tcattaactt accaaacagc atccgccctt tttccccacc ggaaagcact ttagctggct
    24181 ttttgatgtc gtcctggctg aacagcaaac gaccgagaat gctgcgtacc gcctgctcgt
    24241 catcgccttc ctgcttccac tgactcatcc attcgaacac ggtcagatca ttttcaaact
    24301 catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt ttcagaccat tttacggtgc
    24361 cgctgtccgg ttgcagatcg cccaccagcg ttttcagcaa cgttgattta ccgacgccgt
    24421 tggtacccag taccgcaagt ttttcaccga cctccagcag caggttgaga tttttaaaca
    24481 gtggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta cggaacagtt
    24541 tcttatcctg ttcaaaacgg ataaacgggt tctgacggct ggaggctttc acctcttcca
    24601 gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg
    24661 cgctaaagcg gctaacgaaa gattgcaact cagcaatctg cgctttcttc ttggcgttat
    24721 cggccagcag acgttcacgc gcctgggtcg ccgccgtcat gtactcatcg tagttacccg
    24781 gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacagacc atgttaagga
    24841 agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc acctgttcca
    24901 gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg agaatatccg
    24961 gctcagcaaa cagcgcctgc gccagaagca cacgcagctt ccagccagga gcaacttcac
    25021 tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacagt tcaccggcgc
    25081 gagcttccgc agagtaaccg tccatttcgc cgtatttcac ttccagatcg gccactttat
    25141 agccgtcttc ttcactcatt tccggcaaag catagatgcg gtcgcgctcc tgcttcactt
    25201 cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct tcaaaggcaa
    25261 actgatcctg acgcagctta ccaatgcgct cgttgggatc gagggaaacg ttacccagcg
    25321 tcggctcaag gtcgccgccg agaatcttca taaaggtgga tttaccacta ccgttcgcgc
    25381 caatcaggcc gtaacggttg ccgccgccga atttgacgga aatgttttca aacaacggct
    25441 tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc tgaaaagaga
    25501 tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct cgcttaaggt
    25561 ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc actgtgagct
    25621 ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg gcctgccagg
    25681 cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct
    25741 gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac cgacgggagt
    25801 cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca gccgctggag
    25861 tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa cccgggtgtg
    25921 gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct gatcctgccg
    25981 gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta ttactatccg
    26041 aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg caaagatacg
    26101 cctatcaact ggaccaccaa agttgagcat aagaaagcag gcccgacctg gacgccgacc
    26161 gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc taccggctgt cgttccggca
    26221 ggtccggata acccgatggg tctgtatgca ctctacatcg gtcgcctgta tgctatccat
    26281 ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt gcgtctgcgt
    26341 aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt acagtttatt
    26401 gatgagccgg taaaagcgac caccgagcca gacggcagcc gttatattga agtccataac
    26461 ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa tcgtgccaat taccctgacg
    26521 aagagcgtgc agacagtgac cggtcagcca gatgttgacc aggttgttct tgatgaagcg
    26581 atcaaaaatc gttccgggat gccggttcgc ctgaattaat cgtcaaaacg taaaacaaag
    26641 gcggactcat aatccgcctt ttttatttgc cagaccttac caggccggga tcataactaa
    26701 caaaacgtat cccaccagcg ccgcccacaa cagcatcggt attgaataga gatggaaacg
    26761 ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca gcgatcctgg
    26821 taataaacca aagcccccca cgtttaccgc ccatgccagt aataaagacg gaggtacata
    26881 attgagcagc aaaatggtac tcggcacgtt actgatcacc tgcgataaac cgactgccgt
    26941 taaccataac ccgggttcag ataaatgact cacgttaccc aacacgcttt gcaacgctgg
    27001 aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca gcaacgtcca
    27061 gtccacactc aacaccacgc gacgcgccag cagcacaaag cccgccgcca caatcaccag
    27121 tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca gccccagaca
    27181 attccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt attgcagcgc
    27241 ctttccaggg aaacaacacc agcacaacag caggagtgtc agcatcattg cgccagccat
    27301 cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc agataagaat
    27361 attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca gcgcctcaaa
    27421 aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag tcagcggaac
    27481 aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg ccgccagcac
    27541 cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc gccccagcac
    27601 atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc ttaaggtgat
    27661 gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca cgaaaaagct
    27721 taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca gcgtgcgtaa
    27781 aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc gtcgagcgtc
    27841 tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc cggactgacg
    27901 cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc ctgcgccagc
    27961 ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat cagccccatg
    28021 gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag acgagcagcc
    28081 atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag ctcaacgtaa
    28141 tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc aacgtgctct
    28201 tccacgttca ctaactgcgt cacttttttt gctattggcg tacctgcgcg acgactcatt
    28261 gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca cattgtaaac
    28321 cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctatataat atagcctgtg
    28381 ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca agtcaggagg
    28441 tcttatgaat gagttcaaga ggtgtatgcg cgtgtttagt cattctccct ttaaagtacg
    28501 gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc aagataaacc
    28561 ttcagataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg caggcataac
    28621 gcgttttggt ctgaaaaaaa ccactttttg tcggatttgc aatccccttc gcaaaagatt
    28681 tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc cctgcgtata
    28741 ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag gtattgaccc
    28801 ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat ttaaccctca
    28861 aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc tactttttac
    28921 agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac accgctgcgt
    28981 ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg
    29041 gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac ggcgcgccta
    29101 atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc gaaatcctga
    29161 cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc ggcgtaatag
    29221 caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac agcctgtgtc
    29281 tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc tggggtggcg
    29341 tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat gtcatcgtca
    29401 ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga atgaaagata
    29461 cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtagtg atcggtgaaa
    29521 gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca
    29581 gcagcgttaa cggtttgata tttgctgact acattgctgc cagtggctcc acgcagaaat
    29641 cgcttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag gataactttg
    29701 tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag ggccaaatcg
    29761 gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg tacttcctga
    29821 aagaaggtaa ttttgaagca gataaaaaca cgaaagacga agcgttactg gatatgaccg
    29881 ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat ctgatgggct
    29941 cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg caatcgaaag
    30001 aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc aagctgtacg
    30061 atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac cacggtctgg
    30121 cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa tatcagcaaa
    30181 atttccaggt gccttttatg gtcatttcca gcggcgataa agcgcatcgg gtgattaaag
    30241 cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga attaaagcga
    30301 aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg atatacatca
    30361 ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt ttcgacccga
    30421 aaccttaaaa acaaaaatcc gccccgagag gcggattttt atatcaccaa agtgattaga
    30481 agcggtaacc aacaccggca atccaggtgc ctacgtcaac gctacgaata cggctctgct
    30541 cgtaagagaa gtccagagca acgttttcca tcgggttgaa ctgcagacca gcaccgtagg
    30601 agaaaccgta gtcgctggtg tcgtgtttgt aggtcgggta ttcagtggtc tggaatttac
    30661 cataacccac acccactaca ccgtagatgc ttgcccagtc gttaatgcgg taagccggac
    30721 cagcagtgat gccgtagtac tggtttttgt tgtagtcacc agagcttgca gtacggcttt
    30781 tctcggtgta agtgaaagaa ccgatcacac ccagcgggct gttgtcttct tcatagcggt
    30841 atttcaggtt gaaaccgccc attttgttca tttggccctg agcgtcgctc tgtgcgtaac
    30901 cgccagttac ggtagaagtc gcagctacgg aagtacctgc ggtgaaagcc agaactgcgg
    30961 ccagtgctga aagacatgca atttttttca taaccacctc aaatgtgatt caaataagtc
    31021 ctaagtttta aatatatcaa aaattaatgg gaaactcttc gcgatttgtg atgtctaacg
    31081 ggccatttca tgtaacagaa cgtttccata caccgctatc cacctaaatt taaatcactt
    31141 tttcagagaa ctgcgtaagt attacgcatg ttttccctgt cattcatcca gattattcct
    31201 aatcaccaga ctaatgattc catcaatcct ggcgcatttt agtcaaaacg ggggaaaatt
    31261 ttttcaacaa atgctcaacc agcattgggt atatccagta cactccacgc tttacttaag
    31321 tctagatatt tgtgggagaa aggatgcctg gttcattacg taaaatgccg gtctggttac
    31381 caatagtcat attgctcgtt gccatggcgt ctattcaggg tggagcctcg ttagctaagt
    31441 cactttttcc tctggtgagc gcaccgggtg tcactgcgct gcgtctggca ttaggaacgc
    31501 tgatcctcat cgcgttcttt aagccatggc gactgcgctt tgccaaagag caacggttac
    31561 cgctgttgtt ttacggcgtt tcgctgggtg ggatgaatta tcttttttat ctttctattc
    31621 agacagtacc gctgggtatt gcggtggcgc tggagttcac cggaccactg gcggtggcac
    31681 tgttctcttc tcgtcgcccg gtagatttcg tctgggttgt gctggcggtt cttggtctgt
    31741 ggttcctgct accgctgggg caagacgttt cccatgtcga tttaaccggc tgtgcgctgg
    31801 cactgggggc cggggcttgt tgggctattt acattttaag tgggcaacgc gcaggagcgg
    31861 aacatggccc tgcgacggta gcaattggtt cgttgattgc agcgttaatt ttcgtgccaa
    31921 ttggagcgct tcaggctggt gaagcactct ggcactggtc ggttattcca ttgggtctgg
    31981 ctgtcgctat tctctcgacc gctctgcctt attcgctgga aatgattgcc ctcacccgtt
    32041 tgccaacacg gacatttggt acgctgatga gcatggaacc ggcgctggct gccgtttccg
    32101 ggatgatttt cctcggagaa acactgacac ccatacagct gctggcgctc ggcgctatca
    32161 tcgccgcttc aatggggtct acgctgacag tacgcaaaga gagcaaaata aaagaattag
    32221 acattaatta aatttacatt tctgtatggt tatgcataac catgcagaat ttctcgctac
    32281 ttttcctcta caccgtattt atatatcgaa ttatgcaaaa gcatatttat tccgaaaatt
    32341 cctggcgagc agataaataa gaattgttct tatcaatata tctaactcat tgaatcttta
    32401 ttagttttgt ttttcacgct tgttaccact attagtgtga taggaacagc cagaatagcg
    32461 gaacacatag ccggtgctat acttagtctc gttaattact gggacataac atcaagagga
    32521 tatgaaatta tgagtaccgc taaattagtt aaatcaaaag cgaccaatct gctttatacc
    32581 cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag agttgctgaa tcgccaggtt
    32641 atccagttta ttgatctttc tttgattacc aaacaagcgc actggaacat gcgcggcgct
    32701 aacttcattg ccgtacatga aatgctggat ggcttccgca ccgcactgat cgatcatctg
    32761 gataccatgg cagaacgtgc cgtgcagctg ggcggtgtag ctctggggac cactcaagtt
    32821 atcaacagca aaaccccgct gaaaagttac ccgctggaca tccacaacgt tcaggatcac
    32881 ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg acgtacgcaa agcgattggc
    32941 gaagcgaaag atgacgacac cgcagatatc ctgaccgccg cgtctcgcga cctggataaa
    33001 ttcctgtggt ttatcgagtc taacatcgaa taaatccatc gctgatggtg cagaacttta
    33061 gtacccgata aaagcggctt cctgacagga ggccgttttg ttttgcagcc cacctcaacg
    33121 cacttattta gtgcatccat ctgctatccc cagctgatta agtaaatttt ttgtatccac
    33181 atcatcacac aatcgttaca taaagattgt tttttcatca ggttttacgc taaataatca
    33241 ctgtgttgag tgcacaattt tagcgcacca gattggtgcc ccagaatggt gcatcttcag
    33301 ggtattgccc tataaatcgt gcatcacgtt tttgccgcat ctcgaaaaat caatgagttg
    33361 caaaattggc acgatttttt catatatgtg aatgtcacgc aggggatcgt cccgtggata
    33421 gaaaaaagga aatgctatga agtctgtatt aaaagtttca ctggctgcac tgaccctggc
    33481 ttttgcggtt tcttctcatg ccgcggataa aaaattagtt gtcgcgacgg ataccgcctt
    33541 cgttccgttt gaatttaaac agggcgataa atatgtgggc tttgacgttg atctgtgggc
    33601 tgccatcgct aaagagctga agctggatta cgaactgaag ccgatggatt tcagtgggat
    33661 cattccggca ctgcaaacca aaaacgtcga tctggcgctg gcgggcatta ccatcaccga
    33721 cgagcgtaaa aaagcgatcg atttctctga cggctactac aaaagcggcc tgttagtgat
    33781 ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat ctcgacggga aagtggttgc
    33841 tgtgaagagc ggtactggct ccgttgatta cgcgaaagca aacatcaaaa ctaaagatct
    33901 gcgtcagttc ccgaacatcg ataacgccta tatggaactg ggcaccaacc gcgcagacgc
    33961 cgttctgcac gatacgccaa acattctgta cttcatcaaa accgccggta acggtcagtt
    34021 caaagcggta ggtgactctc tggaagcgca gcaatacggt attgcgttcc cgaaaggtag
    34081 cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc ctgcgcgaga acggaactta
    34141 caacgaaatc tacaaaaaat ggttcagtac tgaaccgaaa taataacgct acacctgtaa
    34201 agcgcactgg cagttccctc tcccccctgg ggagaggatt agggtgaggg gcgcaaaccc
    34261 gctccggggc cattaattac cctgaatttg attatttaca ccacggtaac aggaacaaca
    34321 tatgcagttt gactggagtg ccatctggcc tgccattccg cttctgattg aaggtgccaa
    34381 aatgaccctg tggatttcgg tcctcggtct ggctggcggt ctggtaatcg gattgctggc
    34441 aggttttgca cgcaccttcg gtggttggat agccaaccac gtcgcgctgg tctttattga
    34501 agtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt atctatttcg ccctgccgat
    34561 ggcgtttaac gacttacgca tcgacccatt tactgcggcg gtggtcacca tcatgatcaa
    34621 ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg ctgtctatcc acaaaggttt
    34681 tcgtgaagca ggactggcgc tcggtctttc acgttgggaa accattcgct acgtcatttt
    34741 accgctggca ctgcgtcgta tgctgccgcc gctgggtaac cagtggatca tcagcattaa
    34801 agacacctcg ctgtttattg ttatcggcgt ggcggaactg acccgtcagg ggcaagaaat
    34861 tattgccggt aacttccgcg cccttgagat ctggagcgcc gtggcggtgt tctatctgat
    34921 tattaccctg gtgctgagct ttattctgcg tcgtctggaa agaaggatga aaatcctgtg
    34981 attgaattta aaaacgtctc caagcacttt ggcccaaccc aggtgctgca caatatcgat
    35041 ttgaacattg cccagggcga agtcgtggtg attatcgggc cgtccggttc cggtaaatcg
    35101 accctgctgc gctgcatcaa caaactggaa gaaatcacct ccggcgatct gattgtcgat
    35161 ggcctgaagg tcaacgatcc gaaagttgac gagcgcctga ttcgccagga agcaggtatg
    35221 gtgttccagc agttttacct cttcccgcat ctgacggcgc tgggaaacgt catgtttggt
    35281 ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa aactggcgcg tgagctgctg
    35341 gcgaaagtcg gtctggcaga acgtgcacat cactaccctt ccgaactttc tggtggtcaa
    35401 cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc cgaaaatgat gctgtttgat
    35461 gaaccgactt ccgctcttga cccggaactg cgccatgaag tgctgaaggt tatgcaggat
    35521 ctggctgaag aagggatgac gatggtgatc gtgacccacg aaatcggttt tgccgagaaa
    35581 gtagcttcgc ggctgatctt tatcgacaaa ggccggattg cggaagatgg caatccgcag
    35641 gtgttgatca agaacccgcc gagccagcgc ttgcaggaat ttttgcagca cgtctcttaa
    35701 taagacacat tgcctgatgc gctacgctta tcaggcctac aggatatctg gcaacttatt
    35761 aaaattgcat gaacttgtag gacggataag gcgttcacgc cgcatccggc aaaaaagccc
    35821 gcacgttgtc agcaacctgc ttaatatccc ttcctccctt tcacccgaaa gggaggcaca
    35881 ccagattcct ctcatttaaa atcgcccctc ccccagcatc tatacttatc tttttgctct
    35941 gttttctcac tggaggagtc atgcggtgga tcctgttcat cctcttctgc ctgctgggcg
    36001 cacctgccca cgcggtatcc atacccggcg ttacaaccac aacgacaacg gactcaacga
    36061 ctgaaccggc cccggaaccg gatatcgaac aaaaaaagcg gcctatggcg cactggcgga
    36121 tgtgctggat aacgacacct cgcgtaaaga gttgatcgac cagttgcgta ctgtcgccgc
    36181 gacgccccct gcggaaccgg tcccgaagat cgtgccgccg acactggttg aagagcaaac
    36241 cgtgctgcaa aaggtcaccg aagtcagccg ccattatggt gaagcccttt ccgcccgctt
    36301 cgggcaactt tatcgcaata tcaccggctc cccgcataag ccatttaatc cacaaacctt
    36361 cagcaatgcg ctgagccatt tttcaatgtt agcggtatta gtgtttggtt tttactggct
    36421 gattcgcctg tgcgcactgc cgctatatcg caaaatgggt cagtgggcga ggcaaaaaaa
    36481 tcgtgagcgc agcaactggt tgcagcttcc ggcgatgatt attggggcgt ttattatcga
    36541 cctgctgtta ctggccctga cattatttgt cggccaggta ttaagcgaca acctgaatgc
    36601 aggcagtcgc accatcgctt tccaacaaag tttgtttctc aacgcctttg ccctgattga
    36661 atttttcaaa gccgtactac gcctgatttt ttgcccaaac gtggcggagc tgcgcccgtt
    36721 cacgattcag gacgagagcg cccgttactg gggtcgtcgc ctgagctggt taagcagcct
    36781 gattggctat ggcctgattg tggccgtgcc gattatctct aatcaggtga atgtacagat
    36841 aggtgcgctg gcgaacgtca tcattatgct gtgcatgacc gtctgggcgt tgtacctgat
    36901 cttccgaaat aaaaaagaga ttacccagca tatgctcaac tttgcggagc attcgctggc
    36961 ttttttcagc ctgtttatcc gcgcctttgc gctggtgtgg cactggctgg caagcgccta
    37021 ttttatcatg ctgtttttct tttcgttgtt cgatccgggc aacagtctga aatttatgat
    37081 gggtgcaacg gtgcgcagcc tggcgattat tggtatcgca gcgtttgttt ccggtatgtt
    37141 ttcccgctgg ctggcgaaaa ccatcaccct ctcgccacat actcagcgta actatccgga
    37201 gctacaaaaa cggctgaatg gctggctttc ggcggcgctg aaaacggcgc gtattctgac
    37261 cgtctgcgtg gcagtaatgt tgctgttgag cgcatgggga ttgttcgatt tctggaactg
    37321 gctgcaaaac ggcgcggggc agaaaaccgt agatatcctg atccgcatcg cactcattct
    37381 tttcttctcg gcggttggct ggacggtgct cgccagtttg atcgaaaacc ggctggcttc
    37441 ggatattcat ggccgcccgc tacccagcgc ccgtacgcgt accctgctga cgctgttccg
    37501 taacgcgctg gcggtgatta tcagtaccat caccatcatg attgtgttgt cggaaatcgg
    37561 cgtcaatatc gcgccattgc tggcaggtgc cggggcatta ggtctggcta tctcgtttgg
    37621 ttcgcaaacg ctggtaaaag atattatcac cggggtattt attcagtttg aaaacggcat
    37681 gaacactgga gatttggtga ctatcgggcc gttgaccggc acagtggaac ggatgtcgat
    37741 tcgctccgtg ggcgtgcgcc aggataccgg ggcgtatcac atcattccgt ggtcttcgat
    37801 aaccaccttt gctaacttcg tccgcggtat tggttcggta gtggcaaact acgatgttga
    37861 tcgccatgaa gatgctgata aagccaatca ggcactgaaa gatgcggtaa cggaattgat
    37921 ggaaaacgaa gaaattcgcg ggctgattat tggtgaaccg aactttgccg ggattgtcgg
    37981 cttaagcaat accgcgttta cactgcatgt ttcgttcacc acgctgccac tcaaacagtg
    38041 gacggtacgc tttgccctcg acagccaggt gaaaaaacat ttcgacctgg cgggcgttcg
    38101 cgcgccagtg cagacttatc aggtgctgcc tgctccgggc gcgaccccgg ctgaaccgct
    38161 gccgccgggg gagccaacgc tttaacgttg gcgattaaca aaacggcggc gctgctcgtc
    38221 gttcataaag gtccaggcaa taaagcgact ctgcttttgc ccctgggcca tctctttttt
    38281 taccaccttc accgcgccca cgtcggtcag agcacgatac aacggcggta agttttcacc
    38341 acgagatacc agcgaggtaa accacatcac ctgcttcgcg aagcctttgc tctcttcaat
    38401 catctttttg ataaagttga cttcaccgcc ttcacaccac aactcctgtt gctgaccacc
    38461 aaagttcagt gcatcgtctt tgttcagccc caggttacgg cgtttacgct cactacctgc
    38521 ccgcgccgca gcggcggaat cgtggaatgg cgggttacac aaggtcgcgt cgtattgctc
    38581 gtttttgtgg atgatgccgt taaaaatcgc cccactctct ttttgccgac gcagacgaat
    38641 ggcgcggtta agccccggat tagcactgat aatcgcctgc gcactgctta acgcctggct
    38701 gctggtttca ctaccggtaa aacgccagcc atattcatgt acgccaatca gcggataaat
    38761 acagttcgca ccaacgccga tatccagaat gctggcattc gctggaattg ttccgcttgc
    38821 ttcagccagt aaatcggcaa ggtgatgaat ataatccgcc cggcccggta ctggtgggca
    38881 gagaaaaccg tcggggatat cccagttcgc tacggcgtaa aaatgggcca gcaacgcctt
    38941 attgagcgcc ttcaccgcca gcggattggc aaagtctacg ctttgctccc ctgcgggtgt
    39001 aagcgtgagg aattgcctga gttcaggatt gacctgacaa agcgtggcga gatcgtagcg
    39061 gctgtgatga cggttgcgcg gatgcaaccc cggtttctgg gcggacatag cattctcctt
    39121 taagcagcgg cgtaagatac ccgcataacg cgacagggta aatatctggc tgcgtttatc
    39181 attccccaaa accttaacat ggctatggtt tatggcaatt catcataaaa atcagcatta
    39241 tgcatgtcat tgatttgtct tattttgtaa aaatatatcg ttcaaaaaat aagcatatct
    39301 ctgtcaattt tttgcgtcat ttgcatcgcg caggcgacct acacttatca ggcactaccc
    39361 gttgtattta tgaggatgtc attatgaaaa agtgcctcac actactgatt gccaccgtcc
    39421 tgagcggaat ctctctcacg gcttatgccg cgcaaccgat gagtaacctg gacagcggtc
    39481 agctacggcc cgccggtacc gtttcggcaa ctggcgcatc aaacctaagc gatctggagg
    39541 ataaactggc agaaaaagcg cgcgaacaag gcgcgaaagg ttatgtcatt aattccgcag
    39601 gcggaaatga ccagatgtta ggtactgcaa ccatctacaa ataaccccac caacgtaact
    39661 gcattcgcct ctggatgcag ttttcgcctg ttttgtaatc aaaaaacatc aaatatgaac
    39721 gcaatgtaaa taaatgtatt tctttttcgc gcaatgggtg atagaaaatc gcatcaagtg
    39781 ataatgctta tcaaaattat tatcacttgc acgaacacta tcacgggatt aacagtggca
    39841 tcgcatccgc agagatgctt tctcgtggca gtgaaaattt caacatataa gaaaaagtca
    39901 cctgcaaaat ggaaaacaat cgcaatttcc ctgccagaca atttcattcg ctcacgttct
    39961 ttgccggtct ttgtattggt attacgcctg tggcacaggc actcgccgcc gaagggcaag
    40021 ctaacgcgga tgacacgctg gttgtcgaag catcaacgcc ttcgctttat gcgccacaac
    40081 aatctgccga tccgaaattc tcgcgtccgg tagcggatac tacccgcacg atgacggtga
    40141 tttctgaaca agtgattaaa gatcagggcg caaccaacct taccgacgcg ctcaaaaacg
    40201 tccccggcgt g
//
LOCUS       DAJVPP010000026        40157 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVPP010000026 DAJVPP010000000
VERSION     DAJVPP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.026"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(73..1326)
                     /gene="amiC"
                     /locus_tag="OQM34_001366"
     CDS             complement(73..1326)
                     /gene="amiC"
                     /locus_tag="OQM34_001366"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1451878.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1558..2889
                     /gene="argA"
                     /locus_tag="OQM34_001367"
     CDS             1558..2889
                     /gene="argA"
                     /locus_tag="OQM34_001367"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1451879.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2951..4777)
                     /gene="recD"
                     /locus_tag="OQM34_001368"
     CDS             complement(2951..4777)
                     /gene="recD"
                     /locus_tag="OQM34_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1451880.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4777..8319)
                     /gene="recB"
                     /locus_tag="OQM34_001369"
     CDS             complement(4777..8319)
                     /gene="recB"
                     /locus_tag="OQM34_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1451881.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDTVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8312..11200)
                     /gene="ptrA"
                     /locus_tag="OQM34_001370"
     CDS             complement(8312..11200)
                     /gene="ptrA"
                     /locus_tag="OQM34_001370"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1451882.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11376..14744)
                     /gene="recC"
                     /locus_tag="OQM34_001371"
     CDS             complement(11376..14744)
                     /gene="recC"
                     /locus_tag="OQM34_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1451883.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14757..15089)
                     /locus_tag="OQM34_001372"
     CDS             complement(14757..15089)
                     /locus_tag="OQM34_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1451884.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(15074..15481)
                     /locus_tag="OQM34_001373"
     CDS             complement(15074..15481)
                     /locus_tag="OQM34_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1451885.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15478..16041)
                     /locus_tag="OQM34_001374"
     CDS             complement(15478..16041)
                     /locus_tag="OQM34_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1451886.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNR"
     gene            complement(16032..16502)
                     /locus_tag="OQM34_001375"
                     /pseudo
     CDS             complement(16032..16502)
                     /locus_tag="OQM34_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16686..17480)
                     /gene="thyA"
                     /locus_tag="OQM34_001376"
     CDS             complement(16686..17480)
                     /gene="thyA"
                     /locus_tag="OQM34_001376"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1451887.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17487..18362)
                     /gene="lgt"
                     /locus_tag="OQM34_001377"
     CDS             complement(17487..18362)
                     /gene="lgt"
                     /locus_tag="OQM34_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1451888.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18513..20759)
                     /gene="ptsP"
                     /locus_tag="OQM34_001378"
     CDS             complement(18513..20759)
                     /gene="ptsP"
                     /locus_tag="OQM34_001378"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1451889.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKTRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20772..21302)
                     /gene="rppH"
                     /locus_tag="OQM34_001379"
     CDS             complement(20772..21302)
                     /gene="rppH"
                     /locus_tag="OQM34_001379"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1451890.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21987..22676
                     /gene="mutH"
                     /locus_tag="OQM34_001380"
     CDS             21987..22676
                     /gene="mutH"
                     /locus_tag="OQM34_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1451891.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22745..23458
                     /locus_tag="OQM34_001381"
     CDS             22745..23458
                     /locus_tag="OQM34_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1451892.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23596..23814
                     /gene="ygdR"
                     /locus_tag="OQM34_001382"
     CDS             23596..23814
                     /gene="ygdR"
                     /locus_tag="OQM34_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS1451893.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23922..24962
                     /locus_tag="OQM34_001383"
     CDS             23922..24962
                     /locus_tag="OQM34_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1451894.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24995..26185)
                     /gene="lplT"
                     /locus_tag="OQM34_001384"
     CDS             complement(24995..26185)
                     /gene="lplT"
                     /locus_tag="OQM34_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1451895.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26178..28337)
                     /gene="aas"
                     /locus_tag="OQM34_001385"
     CDS             complement(26178..28337)
                     /gene="aas"
                     /locus_tag="OQM34_001385"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1451896.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28923..29954
                     /gene="galR"
                     /locus_tag="OQM34_001386"
     CDS             28923..29954
                     /gene="galR"
                     /locus_tag="OQM34_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1451897.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29961..31223)
                     /gene="lysA"
                     /locus_tag="OQM34_001387"
     CDS             complement(29961..31223)
                     /gene="lysA"
                     /locus_tag="OQM34_001387"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1451898.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31345..32280
                     /gene="lysR"
                     /locus_tag="OQM34_001388"
     CDS             31345..32280
                     /gene="lysR"
                     /locus_tag="OQM34_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1451899.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32267..32959)
                     /gene="ygeA"
                     /locus_tag="OQM34_001389"
     CDS             complement(32267..32959)
                     /gene="ygeA"
                     /locus_tag="OQM34_001389"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1451900.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33088..34506)
                     /gene="araE"
                     /locus_tag="OQM34_001390"
     CDS             complement(33088..34506)
                     /gene="araE"
                     /locus_tag="OQM34_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCS1451901.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34821..35582)
                     /gene="kduD"
                     /locus_tag="OQM34_001391"
     CDS             complement(34821..35582)
                     /gene="kduD"
                     /locus_tag="OQM34_001391"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1451902.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(35612..36448)
                     /gene="kduI"
                     /locus_tag="OQM34_001392"
     CDS             complement(35612..36448)
                     /gene="kduI"
                     /locus_tag="OQM34_001392"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1451903.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(36735..37916)
                     /locus_tag="OQM34_001393"
     CDS             complement(36735..37916)
                     /locus_tag="OQM34_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1451904.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38171..39400
                     /locus_tag="OQM34_001394"
                     /pseudo
     CDS             38171..39400
                     /locus_tag="OQM34_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
     gene            40035..>40157
                     /locus_tag="OQM34_001395"
     CDS             40035..>40157
                     /locus_tag="OQM34_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1451905.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSL"
BASE COUNT         9969 a        10858 c         9746 g         9584 t
ORIGIN      
        1 ttattgaaca ccctaacggg tgtttttttg tttctgatct cccataaaaa agcgccattc
       61 agcgcctttt tatcatcccc ttctcgccag cgttgcccca tcggcaaaat acgctttaat
      121 ccccgcaaga atagactccg caacttcctg ctggaaagtc gccgttttca gtttacgctc
      181 ttcctcaacg ttactgataa acgccgtttc gaccagaatg gagggaatat ctggtgcctt
      241 tagtacggca aacccggcct gttcaacttg atttttatgc agcttgttga tttttccgag
      301 cttattcagt accgctttac caaacttaag gctgtcagca atagtcagcg actgcaccat
      361 atcgaacatg gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc
      421 cgaggcgttc tgggtttgtg ccagatattt tgccgcagta ctggttgcgc ctttggttga
      481 gagcgcaaac accgaggaac cgctcggctg acgactggta aaggcgtcgg catggataga
      541 gacaaacagg tcagcacgct gtttctgggc ttttgctacg cgcacttgca acggaatgaa
      601 gatgtcttca ttgcgcgtca tgtacacctt catattgccc tcttcctcga tcagagagcg
      661 caaacggcga gctatttgca ataccacatc tttttcgcgc gttttgtatt tccccaccgc
      721 accggagtct tcgccaccgt ggccagggtc aagcataatg acaatcggac gatcgcgccc
      781 tgctttaccc ggttgtggac cactttgcgc tggcggcacc tgcttttcga ggtcgccttt
      841 gttgtaatcc tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata
      901 gaggtccatc accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg
      961 ttttacgttt tgctttaatt caaaaaccat gcgtacggtt tgcgggtcaa attgcccgac
     1021 gcgcgccgac ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac
     1081 cgagttcagg tttacatctt cgatatccac caccacgcgt tcagggttac tcaacgcgaa
     1141 ctgcttatat ttcagctgac gatttgattc taccgtcacg cgggtgtagc tggacgcagg
     1201 ccagacgcgc accgccacga cctggctaac cgcagccagg ctgacctgac ttacgctcaa
     1261 tagccacata gcgcccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc
     1321 tgacatgcct ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg
     1381 accggaaact ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt
     1441 tatattcacg gcattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct
     1501 gcgaaaaaac agaataaaaa tacaataatt tcaaataatc atgcaaaggg gtgtgccgtg
     1561 gtaaaggaac gtaaaaccga gctggtcgag ggattccgcc attcggttcc ctatatcaat
     1621 acccaccggg gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat
     1681 ttctccagta tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg
     1741 gtctatggcg cacgtccgca gatcgacgca aatctggctg cgcatcacca cgaaccgctg
     1801 tatcacaaga atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg
     1861 ggaacattgc aactggatat tactgctcgc ctgtcaatga gtctcaataa cacgccgctg
     1921 cagggcgcgc atatcaacgt cgttagtggc aattttatta ttgcccagcc gctgggcgtc
     1981 gatgacggcg tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgctc
     2041 catcgtcaac tggaaagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact
     2101 ggcgagagct ttaatctgac ctcggaagag attgccactc aactagccat caaactgaaa
     2161 gctgaaaaga tgattggttt ttgctcttcc cagggcgtta ctaatgacga cggtgatatt
     2221 gtctccgaac ttttccctaa cgaagcgcaa gcacgggtag aagcccagga agagaaaggc
     2281 gattacaact ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc
     2341 gtgcgccgct gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc
     2401 tcacgcgacg gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca
     2461 acaatcaacg atattggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt
     2521 ctggtgcgcc gttctcgcga gcagctggag atggaaatcg acaaattcac cattattcag
     2581 cgcgataaca cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg
     2641 gaaatggcct gtgtggcagt tcacccggat taccgcagtt catcacgggg cgaggttctg
     2701 ctggaacgca ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc
     2761 acgcgcagta ttcactggtt ccaggaacgt ggatttaccc cggtggatat tgatttactg
     2821 cccgagagca aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat
     2881 ttagggtaag cgatgaaaat caccggatgc ggcaggcgta acactcgtgc gtcgcatccg
     2941 gcaattacgt ttattcccgc gaactaaaca acgccgccag accactgcgc cgctcagtac
     3001 gagtggcgat tgccgcactt aatatacgct catcggcata cagcgacaga cgacgacgcg
     3061 cgcgggtcac cgcggtgtat accagctctc gcgttactac cggcgtgcgt tggctcggta
     3121 aaatcaacgc cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg
     3181 tagtttcgtg ctctggcaag cgactcggtt gcacagactt aatattgccg tccggcatcg
     3241 caaaccagac gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca
     3301 acccaagcgc gctgtcatta cgggcaatca tcaccggtcg gccttcgtac caacgagagt
     3361 gcggatgacg atgaattttg cgcttctgtt gcataaactg ttcaattcgc tcattcagtc
     3421 cagccacgcc aaacggccct tcccgcaggg cgcacaaaag ctggtactca ttgaacgcct
     3481 gaatgattaa atccggctcg gcacgcgctt gcagcagatc cagataacgt ccgtaacccg
     3541 caagagcttc ctcaagcatc gcaatataat cttcgccgct ctgtaaaagt cgtttttcga
     3601 tatcagtaaa atcctgctga aaaacggttt tcactgccgt tttatcacca cggttgatcg
     3661 ccgcagctaa ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc
     3721 agagactgtc gcgcaaagat gccgcttctg tgccggttcc tgccggaacg tgagtccccg
     3781 tcaggcggct tagctgcctg gcacgctcgg cggtaaagcc cgcgttggca taagcgcaga
     3841 tatcgcccag cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga
     3901 tcactcgcgc atgatctggc aaggcgtcga tcagtctcga catcataggc agatcgatca
     3961 ttgacgcttc atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta
     4021 aacgctggct acccggctgc gcgcccagca atcggtgcaa agtgctggca tcttccggaa
     4081 tgcgtttctt ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg
     4141 ttaagcgcgc ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt
     4201 cggccatttg aattaacgct gccagcaact tcgccacggt ggtcgtttta ccggtgcctg
     4261 ggccgccgga aatcaccgag atccgccgcg tcagcgccac tgccgctgca actttttgcc
     4321 agttaatttc atcgcttact ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat
     4381 caacctcaat ggtatgattc acctcattga agaagcgcgc taccgtccgc tcgttacacc
     4441 acatgcgatt caaataaaga cgatcgccac agaggatcat cggcgtgggt tcatctcccc
     4501 tgctgaccgc ttgagaagcc agcaagcatt cttcccaatt ttgtagctca ccgatttcac
     4561 tgacacaggt cgccaacagc ggattcgacg cctcgttatt ttccagtcgt gaaagcggca
     4621 aacagacgtg tccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag
     4681 gatgttcatc tcccgccacg gtcagggcaa attgcacatc cagcgggcgt agctgtttgt
     4741 gctccacagc ttccagtaat tgcttttgca atttcattac gcctcctcca gggtcatacc
     4801 ggcaaacatc tcatccatca gggcaatcaa cccggcgttg ggtcgggttg tgtaaatccc
     4861 ttgttgcgga tgttctttat caacgccacg caggaacaga tagataacgc cgccaaagtg
     4921 aagatcatag tcgtaatcag caatgcgatg gcgcagataa cgatgcagcg ccagggtata
     4981 aagctgatat tgcagatcat agcggtgtgc ctgcatcgct gctgccatag cctgttgggt
     5041 gtaagccgaa ctgtcttcac ccagccagtt ggatttatag tcgagcaggt aataacgccc
     5101 ttcgtggcgg aataccaggt cgataaagcc ttttaacatg ccgcgtacct gcatgaactc
     5161 cagcggcggg cagccagcgg atagcgggtc aaactggcga attagtgcat caagctggct
     5221 ggcgatgagc ggttcgctaa tcggcaggta aaactccatc tccacctgtt tatcgcggtc
     5281 ggaaagctga ctcaggctaa cacccgtttc attgagaggt gcctggagga cagccgtgat
     5341 ccactcggtc aacaccggtt cccactgcga ttcaaagccg ccgagttcca gtttttcctg
     5401 tacccagttc gggtcaaccg gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa
     5461 tgtccccggt gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac
     5521 gctgaccacg cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg
     5581 accacgctgc tgcaagccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg
     5641 tagtgtcctc gcattcagtt ctgcagttaa agcatcatta acctgccagg gctggttatc
     5701 accagtttgt gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg
     5761 aagccctgcc gcatcttgcg gttccccttt ttgcagcaaa cgcccgagcg cactttggtg
     5821 gacgtcggtg tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact
     5881 gcaatgccaa accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg
     5941 ttcgacctcc gcgaggtcga cgctttctgg cgcagcatta agatccaaaa ctgcctcaaa
     6001 cgagtggcga tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa taaatggcaa
     6061 ccagaccagc ggatattcca gccctttcga tttatggatc gtgacaatct gcaccagatg
     6121 tttatcactt tcgaggcgca tttgttggct ggaggcatta ctgtctggct cgaggatatg
     6181 ttgcgataac cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgaag
     6241 cagttcgctg atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa
     6301 gttttctgca atgttacgcg ccgacatcag cgcccgcagc atcggcataa cgccacgttt
     6361 gcgccagatt tgccgataac catcgaactc ttcgactacc gtatcccacg catgttcgtc
     6421 attattcagc gtttcaatat ccagcgcatt cagccccatc attgacgttg ccagcgcact
     6481 gcgcagggtg ttctcacgtt cgggcgtcat caccgcctgc aacaaccaaa gcatttcctg
     6541 cgcttccaga gtttcaaaaa cactgtcgcg gttcgaaagg taaacggaag ggatttccag
     6601 caacgttaag gcatcgcgca cctgggcggc ctcctggcgg ctgcgcacca gcacgctgat
     6661 gtccgaagca cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg
     6721 tccggcttgt agccagtcgc ggatttgcgc agcacatacc tgcgccatgg tactttgata
     6781 gtcgccaacg ccgcagcttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc
     6841 acctttaaat acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg
     6901 tatttcgcga aacatgaacg tgtcatcagt ctggctgaaa agcttgttca cactgttcac
     6961 cattccaggt gcggagcgcc agttggtgtc taaagtgtag tgggcgtgaa cttcactacg
     7021 cgccttcata taagtgaaga tatccgcacc ccggaatgca tatatggcct gcttcgggtc
     7081 gccaatcagc aacaatgcgg tttccggctg atggtgccag atacggcgaa aaattcggta
     7141 ctgctggggg tcggtatcct gaaattcatc gatcattgcc accgggaatc gcgtacggat
     7201 cgccgctgcc aacacctcgc cgctttcgct acgcagcgcg gaatcgagcc gacttaacat
     7261 gtcatcaaaa cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc
     7321 agccaatgcg cgggtgatca acagatcgcg gatcgacaat ggttctgcaa gcagttgatc
     7381 gatcgcctca aacagtggat gtcgcggggt ttcccccccg gccttcgtgc gatcttctaa
     7441 gaaacgctgg gagaattttt ccagcgactc cggcaactga taactgtttg tctcttcttc
     7501 tgcccaggcg ctgatctttt cgatccattt agcctgattg ctacggttaa acttgcgtcg
     7561 atcaatacca gaagattcga tcagcgcatc cagttcaccc actgcgtcgc gccactgctg
     7621 ttttaccgta tcaatacgcg ccacaatttg cgcgtggcgg gaagccagcg tttcatcatc
     7681 gggcggcggt gctttgataa ccggcggttc gccttgcaga taacgattaa tatcgcgcag
     7741 caacgcctgc ggccctttcc aggtttcaaa gacgacctgg gctatttcac gcggcagcgg
     7801 gtagcagtgg cgacgccaga aatcggcgca ggcctggtag cgtagcagag actcatcttc
     7861 aatcagctgc tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg
     7921 gcaaaagccg tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa
     7981 ccactgcgcg gcttgcgctt tatcgtcgat ctcttccagc aggcgttcgt acagtggatt
     8041 gtcggtggtt tcacgcagac aggcgatgcg caactcgtgg atattgctac ggatacgacc
     8101 gcgcaattct gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacgatcag
     8161 cgggcgggga aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat agagcgccgc
     8221 aatcgtaaag gttttgcctg tgccggcaga ggcttcaatc aggcgctcgc cctgtaaggg
     8281 caagcgcaaa ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca
     8341 ttgtttgctg caacgcgctg acgttctccc acactttcca gccttcagga tgcacatatt
     8401 cggctttccc gttctggctg ccggaaatct gcgacagaat agccatgcct tgcggctcga
     8461 ccaccgcctg atggaagaaa tcagcaagtt tttgcggcgt cagcagtttt atctgggcca
     8521 cgattttatc acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttcg
     8581 atgcttcttc gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga
     8641 tttgcgcaaa ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg
     8701 ccttgtaacg ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca
     8761 tgccccactg acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt
     8821 cggtacgcaa ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg
     8881 ctgagctggt gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg
     8941 tgctgttacc ggctttttca aagatgacgg attgtttttt atcgaccact acatctttgt
     9001 ttcgacacca ctctgaacca tcagcgccca actgtttttg cacatcgcgt gccagcgttg
     9061 ttgcctgggc ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta
     9121 aggcgtcgcg ataggccagc acctctttca acgtaatgga gggcaaaatt ttacgccgtt
     9181 catctcgcga gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcaa
     9241 acgctttacc cttttctgcg gaatccatca tctggttata ccaggacttc gcctgctcaa
     9301 gctgctcttc cgtagcggta tagctaaagt acccctcgag caatgcctgg aacagctgcg
     9361 gcagacgctg ggtgtaacca ttagcattaa tcataaggcc gttgttagcg ttggtggaaa
     9421 aacttatgcc accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat
     9481 aatcattgag cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca
     9541 aaatcaggct gacatcagct ttgggctcgc tggcaaaata acggcttggc gcatacacca
     9601 cgcgcagatt cgactcatca acaatcagct ctggatggtc gtatttcttc tctgacttaa
     9661 tcagcgagaa atcatcagga atataagggt taagctctgg caaagagagc gcaatgtcgg
     9721 cggctttttt ctgccagtcg gcgaaagttt gtgcgctgat tttatcgacc tgatacggcg
     9781 catcgacaaa gtaagccgtt ttgttgtgcg gctctttcgg gctgatatac cagatacgcg
     9841 cattctgcgg cgtcatcatc gccagacgtt cctttactgc tttagcatcg taccgatcgg
     9901 caatattgac tgcatccagc gtatgctcaa caggaacgcg aatcatggta tctgccagcc
     9961 attcgacgta atccatatca cgggtgatcg acggataacg gaagtcgata tccagcacat
    10021 tcgccagttc atcgaagtat tgtttatcaa tgcctttttc acgtaacaga ttgagatagc
    10081 taaaaattgc cgccacaacc tgatcgcgat tagccaggcc tttatcagtt aaagacgcag
    10141 agatcgctaa tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct
    10201 caactaatcc ctgcttttgc agccagtcag aaagtgtacc tgggctgcga ttgccaatca
    10261 gataggtaat caattcatcg gttttactac ggaacttcgc tgagttgtta tcgatgcgaa
    10321 actcaacgcg caacacttta cgcggcagcg cagggacgta atgaatgata atgccctttt
    10381 gcgcgtcggt gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc
    10441 gaccaaaggt gtccgccgcc atttttgcca actccggcag cggtttatta ctgtaaataa
    10501 ccgccttcat caaattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca
    10561 ccggattacc aggtttgtcg cttaaagttt cgaggttacc accagaaaac tttgaaccgg
    10621 ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac
    10681 gcgccatggt taattcagcg ttcactgcat tacgctcacg ttcggcgtat ttcttgtcga
    10741 gcaagggttc tgcaatagca tcggccaggc ggtctaccgc accgggcaag gcgtcgttct
    10801 caacttccag atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt
    10861 gcattttgag atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac
    10921 tcatatgttc aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa
    10981 cgggcactac cagcgccgag agcgatttaa ctgcctgcgg atcagaaacc agcaagacca
    11041 ccataccgtt atccagacgt atagcctgat actggcggtt atctttatca cttttacgga
    11101 tggtttcctg aatcggctgc catcccgttt ctgcctgact taatggtgcc cagagggcaa
    11161 caaacaacaa taatgctttg aaccaggtgc tgcggggcat tcacggacct cataatcaac
    11221 tttattttct ggacagattc aacaggttaa cgcaaatcat ctgccagaat ttaatcttgt
    11281 gctgcacgag tcagcctatg tttatataac catcagtccg tgactggtgc gcatcataaa
    11341 gtaagcggat agattgcgca atttttatac aacactcatg actgattaaa gcgaaacagc
    11401 ggtaacagga aacgttgcga ctgttcaacg atggcctcca ttgtctctgg tgttaattgc
    11461 cgccagagcc gttgatacca gatatcatca ccttcgccac gcaccatcat gttgccttcg
    11521 taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca
    11581 tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc
    11641 aatggcgcgg acattccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc
    11701 tgttcggctg caagcggcgg aaaacgccac tcaccgtctt tgcgtagaaa aaggcgactt
    11761 tcgccattac caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgc
    11821 gccacactta ataaagaggg acgccagcgc aacaggccat ccggctgcac ctgcggcaac
    11881 cagccagtta tctgcacacc gttgcaggcg agatcaattt ccatactctg ccccggctgg
    11941 cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa
    12001 atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcggcg gaacaagcgt
    12061 tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta
    12121 agtccttcca gaataaatgg ctcggtgtct gggatttcgc tgtcttcagt atggaagttc
    12181 acctgcaaac gcatctggaa gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt
    12241 tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct
    12301 ttaccagcct ggctggccgc aggtagccat tcacgagcat agctttgtcg ttcgcctggc
    12361 tggtagtttt gcggatcaaa cggcatccgg gtatggaggc aagtaagatg cgcttttacc
    12421 cttgcctcgc tttcatcaca gttgagcgct tcatcgcccg gtagataatg actttgcccg
    12481 atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg
    12541 gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac
    12601 aggtagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg
    12661 tcaaagccca atggcgcaag ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa
    12721 accactttga acggaattga acgcattggc atcagagtac aaatgttaac cggtccggcg
    12781 agaaaacgct ggctgatacg ttcttgatcc agacgctgtg ccagttcatc acgcaatagt
    12841 gacagcggca ccgcgtcgcc atactgcgca cctaaacctt cggcgataat cgcctgccat
    12901 tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gaaggcgttg
    12961 agcatatcgc gacaaaccgg caaccactct tctagcggac gctcctgtgc cagcccgcga
    13021 cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag
    13081 ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg
    13141 tagcccagca acatacgcgt caggccaaat cgccaggtgt gttgtccggt ggcggggagt
    13201 tccagctcgc gaacgttgtc gtcatctatc ccccaacgaa tgccggattc gttgacccac
    13261 tggcgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca
    13321 tccagcagcg ccagcacatc ctctgacaca aagcgactgt caggcagtga taacaggctg
    13381 ataaacgctt ccagtaccgg atgtgactgc cgcgcacgac ggtcggaaat ggcgtaaggc
    13441 aggtaacgat ccgcaggtgc gctaccaaac actgcctgaa taaacggact gtagctgtcg
    13501 atatcagcca ccatcacgat gatgtcgcgc ggagtaagtg tcggggcttc ctccagcatc
    13561 gccagcaagc gatcgtgtaa aacttcaact tcacgctgcg ggctatggca aacgtggaat
    13621 gtgatactgc tatccagtgg atcaagcggg cgtttgttat cgctacggga aaactcttcg
    13681 atgttcacac ccgcaacggc gcggttttcc agttccagaa tgtcagactg aatgttatgc
    13741 agcaggttat ctggcgtcac atcgacaaaa gcgtccagct cctggctgct ctccaggtca
    13801 gaaaggagat aaatgtagtc gcgcccaagc ttcccccatg aggccagcag cgagttgccg
    13861 acatcctgtt caccatcgct gttaaagagc tgtccggcat tttcgctatc gcgaaatagc
    13921 ggcaattcgc gatcttcaaa actgtgtcga cgctgacgag tcagcagttt cgccagataa
    13981 gcaggatctt taatatcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt
    14041 tcaatatgtt tacccagcgc ctggagcgcc tggagataaa caggcggtaa cgcggaaata
    14101 ccgcatataa agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt
    14161 tcgataaagc gctgatagag attcgcgcgg tgccagcgcg gttgtccgag ttcatgggta
    14221 tattccacca gcgccttcca caacggggct tgccaggcct gtgcttctcc cagcccttca
    14281 accaaatgtc ctgtttccca ctgtgccagc cagtccggac gatagaccag atactggtca
    14341 aacaggtccg ccgcttttga ggaaagctgg aacagttttc gcttgtcgct atcgtcagtc
    14401 agataatgcc gcaacagggt aaagtcttcg cgctccagca attgcggcag cagagtcatc
    14461 agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatttc cggtaacacc
    14521 cggacgaaca tatcccagat aaagctcgct ggcagcggaa aatcaatgtt tgccgcaata
    14581 ccaaactttt gcgacagggt catttgcagc cactgcgcca taccggtact ttgcaccaga
    14641 atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac
    14701 gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac
    14761 tgacgattcg ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg tgaaactagc
    14821 gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac ctgccagttg
    14881 gcaggtggcg aaatcgcgcg cagttgcgtt tgctgccaac catgccgcca gagctgttgg
    14941 tactggtttc tgctggcgag actgttcatt gatgttcgct ggatacccga taacgcagtg
    15001 acaatcatca ccatcagcac catcagcacc atcgcaacca ttacctccgg caggctaaag
    15061 ccttgttgat tcttcaggga agctgacata atgccccctc tttcagcgga caaaaatcgc
    15121 tccagccgcg tggcgaaaaa acaatttttc catcaatgac ttcgcctgtt cgccacaacg
    15181 aaacgccttc ataaccggca atcaataagg cttcattatc tgccagtaaa cgcaaacaaa
    15241 cctgggcatc ggttccagcg tactgcgagc actgaactgc tggctgcgtc tgccaggagt
    15301 gcatttttcc ccacgccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt
    15361 ccatgctcac gcgagaggca aaactgcgat cctgctgact cattccttgc aatagcaagc
    15421 tacccaaaac cagcagcatc aggaccagag ccagtgacga aacacctttt tcgcggttca
    15481 ccggttgaat cctgtcacgc tatagctggc atccaccacg gtttgcggtt cagacttact
    15541 ggcagcacgc atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac
    15601 ctgaaaagtg tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc
    15661 acaggatgtc gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa atccaatctg
    15721 gtcggactct tttaccggtt cgcgatccca gataccgtta ctgttcgcat cccactgcac
    15781 aatgatacag tcaccctgtc cgacaatttc cagcccttcg ccggtacagc tgccatgaca
    15841 ataacccgcc ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc
    15901 cagtgccagc ttacgagtgc tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc
    15961 agcccccagc aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa
    16021 accttgctct tttacaggca tccttctgtt tctccttgct gataaagccg gagtcttccc
    16081 cacggcgaaa ccaccagcca ccactcgccc gttgagtttt tgaagcgaat atgcccggcc
    16141 catgcggtat tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttcgact
    16201 tcgggccagc gtggcacaaa gaccaatggt gaactgccat gacaggtatt ggccccagca
    16261 gcggaactca caaggcacca taacgtcccc tccctgataa cgctgatact gtggtcgcgg
    16321 ttatgccagt tggcatcttc acgtaaatag agcaaatagt cccgcgccta gctggcggtt
    16381 tgccaaagcc gttgcgactg ctgccagtat tgccagccat agagtccact tgcgcttagc
    16441 atgaccaaaa tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc
    16501 atgccggcag tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggcg
    16561 catcttccgg actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga
    16621 agaatttaac ggagggtaaa aaaaccgacg cacactggcg tcggctctgg caggatgttt
    16681 cgtaattaga tagccaccgg cgctttaacg cccggatgtg gatcgtagcc ttcaatctca
    16741 aagtcttcga aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc
    16801 agcggacgcg gttcgcggct taattgcaga tgagtttgat ccatatggtt gctgtacagg
    16861 tgcgtatcgc caccggtcca gacaaaatca cccacttcca gatcgcactg ctgcgccatc
    16921 atatgcacca gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa gacgtcacag
    16981 gagcgctgat aaagctggca agagagtttg ccgtctgcca catagaactg gaagaatgca
    17041 tggcacggtg ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg
    17101 cggcgcgaat ccgggtcgtt tttcagctgg ttcagtaccg tagtaatctg gtcaatatga
    17161 cgaccatctg gcgttggcca ggcgcgccac tgtttaccat acactggccc gaggtcgcca
    17221 ttttcatcgg cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta
    17281 gtatcgccct gcaggaacca cagcagttcg tggatgatgg aacgcaggtg gcaacgtttg
    17341 gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa
    17401 agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc
    17461 agttctaaat actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat
    17521 gcccagacca tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgccccatg
    17581 ctgatgtact gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca
    17641 atgatgcgaa acgcgccgta gccaatcagg aacaagcctg agacagctcc cattgggcgc
    17701 ggtttacgaa tatacaggtt gaggataata aacagcacca caccttccag cagcagttcg
    17761 taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc
    17821 gggttggttt gcagcagcaa aatatcttct gtacgggagc ctgggaacag catggcaaac
    17881 gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc cagacgcccg
    17941 gcaccaagac caaacggaat gagtggtgcg ataaaatcag agacctggaa gaaggaacgt
    18001 ttagtacggc gggcgaagat aatcatcacc acgataacgc caatcaggcc gccgtggaaa
    18061 gacatgccgc cgtcccagac acggaacaga tacagcggat cggccataaa ctgcgggaaa
    18121 ttgtagaaca gaacataacc aatacgtccc ccgaggaaga cgccgaggaa gcccgcatag
    18181 agtaagtttt caacttcatt tttggtccag ccactgcccg gacgattcgc ccgacgtgtt
    18241 gccagccaca ttgcaaaaat gaaacccacc agatacatca ggccgtacca gtgaagcgcc
    18301 acgggtccta ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc
    18361 atctgtcacc acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc
    18421 gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg
    18481 gataccgtta aaagatatgt atatgatccg cgctataacc ctccgcgaat cagcccgccc
    18541 atgccgcgac gctccataaa ggctgcaacc tgatgacgaa cttcggtcgc cagttgcgct
    18601 tccagactac gctgcgcaag attttctgct tcggcaaaat caatgcgccg cagcaggtat
    18661 tttacccgcg ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg
    18721 attgccacgc acatgggatc gcccgccatt tcaccgcaca aacgaagatc gataccatgt
    18781 atttccgctt cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtcg
    18841 taaatgttcg ccacccgggt attgttgcga tcaacggcca gaatgtattg agtcagatcg
    18901 ttggtgccaa cagagatgaa atcgacccgc tttgccagat gcggcagcat aaataccatt
    18961 gacggcactt ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc
    19021 tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt cgtcaacttc atcgaggctg
    19081 gtgaccatcg gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc
    19141 cgcacctgga tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc
    19201 aggcatggat tctcttcgct gatcggcatg taaggcagct gcttatctgc tccgacatcc
    19261 agcgtacgca aggtgacggg tttatcattg aacatttgca gcatcccctg atactgcgcc
    19321 acctgttctt cttccgacgg gaaaccactt tgcagcatga atgggatttc agtgcgataa
    19381 agaccgatgc catcaataca gctgcccagt ttttcttcat gttccgggct taaaccagca
    19441 ttgagcatga cttttatacg ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct
    19501 tccgccagac ggctaagctc aatctcttca ctaattagcc gctgatattc ttgcagcagt
    19561 accggctccg gatcgaccag caattcaccg cgatagccat caacgatcag cgtcctacga
    19621 tgcagcaccg aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc
    19681 atgatcgcag catgggagtt ggctgcgcca tctcgcacga caacaccgac taagcgatcc
    19741 tggggcagct cagcaagagt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc
    19801 ggccaggcgt tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgacccagc
    19861 gcacgtaaat cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttcg
    19921 gcaaattttt caatgaccgt ttttaccgcc cactctgcca ccgagccttt atcaacctcg
    19981 gcaaacaatt cgcgacgcag ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata
    20041 gccgccgttt ctttttgtgc tccggcggca aagcgtttgc tgtagcggcg aaactcgttt
    20101 gccgcttctt ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat
    20161 gcctgataca cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgcc
    20221 acaccaggtg ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc aaacagggca
    20281 gtcaactgcg actgagaaag aatagctgcc atctgggtgg caagcgtcac caggaaggat
    20341 tcttcacttt cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac
    20401 tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttacggag
    20461 gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc
    20521 ctgccaacca ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtgtt
    20581 tttttcagcc cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag
    20641 accgaacaga cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc
    20701 tcattcaggc gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata
    20761 attggcgtga cttaacctct tttacgtcga taagcagatg cgttttgtgg ttttggcgta
    20821 ttttcctgca gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca
    20881 cgtttaaatg acaccacctg tctgaccgga taccagtaac ttacccatcg ccagccgtca
    20941 aactctggtg tactgctggt ttgcatattg atttctgcat cgccgcttac cagctgcaag
    21001 agaaaccatt tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt
    21061 ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg
    21121 cttaatccta cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg
    21181 ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc
    21241 tgcccctggc gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc
    21301 accggactac ctcaaaataa agctttatat acgaatgatt gtttcatact ccaggaagac
    21361 ggtaaaccac tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg
    21421 tgcagagtta taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat
    21481 agagttgtga agaactacgg aattactacg ggaaaacccg aagcattctg aataagcccg
    21541 atatagaaat tttaaaatat tcattattag tcaatgcatt acatgtaatt tgagtaatga
    21601 aaaagagtaa ttcgtgaccc aggtcacacc tctcatttac gggttggcga aagatcaacc
    21661 aatgccgtat ttatccacag aatgtgccac taagttaagc actgaaccac taaaaactgg
    21721 agtttcgtcg cacgtcaagg ctgtaaatga aaacagtagt ggaggttttt cacagttatc
    21781 ccagctttct gtggataaca tggtgtaaga tcctgtttat tttcagtgac cagatttgga
    21841 aaacctattg cagtgttgcg caactcgatt accggcagcc taaaaagcaa tataaatcag
    21901 agaattgaac aacacatgtg gaaaaagtta cactgcgaat attcggcaca taattactgt
    21961 ttgtttttta accaaggtat catgacatgt cccaacctcg cccactgctc tctcctcccg
    22021 aaactgaaga acagttgtta gcgcaagcac agcaactttc tggttataca ttgggagaac
    22081 tggcggcact tgccgggctg gttacgccag agaatttaaa acgcgataag ggctggattg
    22141 gcgtgttact ggagatctgg ctaggtgcca gcgcagggag taaacctgag caagattttg
    22201 ctgctctggg cgtggaactt aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa
    22261 caacattcgt ttgtgttgcc ccgttaacgg gtaatagcgg ggtgacctgg gaaaccagcc
    22321 atgtgcgcca caagctcaaa cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc
    22381 cgctggcgca gcgtcgcgta ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc
    22441 gacagctacg cgaagactgg gaagaattaa tggatatgat tgttctcggt caggttgagc
    22501 ggatcaccgc tcgtcacggg gaatatttac agataagacc gaaagcagcg aatgcgaaag
    22561 cgcttaccga agccattggt gcccggggcg aacggattct gacgctgcca cgcggctttt
    22621 atttgaagaa gaatttcacc agtgcactac tggcccgtca ttttctgatc cagtagccat
    22681 cgctttgacc tgccgctttc cgggcatata attaccgctt catttttttg gcagggcttt
    22741 ttagatgtta tttgcatgga taaccgatcc taacgcttgg cttgcgctcg gtacgctgac
    22801 gctgctggag atcgttcttg ggatcgacaa tattattttc ctttctctgg tggtggcaaa
    22861 gcttcccaca gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat
    22921 gcgtctggcg ctgctggcat caatctcttg ggttacgcgc ctgacaaatc cgctttttac
    22981 aatattcagt caggaaattt ctgcccgtga tttgattctg cttctgggtg gcttgttcct
    23041 tatctggaaa gccagcaagg aaatccacga atccattgaa ggtgaagaag aagggctgaa
    23101 aacacgcgtt tcatcattcc tcggcgctat cgtgcagatt atgctgctgg atattatctt
    23161 cagcctcgac tcggtgatta ccgctgtggg tctgtcagat cacctgttta ttatgatggc
    23221 agccgtggtg attgccgtag gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt
    23281 cgaacgccat ccttcggtaa aaatgctggc gctctctttc ctgattctgg tgggctttac
    23341 cctgattctg gaaagtttcg acatccacgt accgaaaggt tacatctact tcgcgatgtt
    23401 cttctctatt gcggttgaaa gcctcaactt gattcgcaac aaaaagaatc cgctctgata
    23461 ctccgtacgc tctcctgcgg gagggcgtac tcactccttt taccctcaca gtttaagatt
    23521 ttcctgcttt caagatatat aacgtcggtt tataaacaga ctattatcat aggtaggcca
    23581 aatgaggata accggatgaa aaaatgggca gtaataattt ctgcagtcgg actggcgttt
    23641 gctgtttccg ggtgttccag tgattacgtc atggcgacca aagatggccg tatgattttg
    23701 accgatggaa aacctgaaat tgatgatgat accgggctgg tgagttatca cgatcagcaa
    23761 ggtaacgcga tgcaaattaa ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata
    23821 aggtcagcat ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat
    23881 ttttatattc cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac
    23941 cccacagttc gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga
    24001 acagcgaagc cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta
    24061 tcgacgttgc cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa
    24121 cctacgtcgg caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca
    24181 aagtgagcgg accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc
    24241 ggaagaatat ccgcgaagcg ctgcatgaca gcctcaagcg cctacagact gattacctcg
    24301 atctttatca ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata
    24361 gctggacgga ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt
    24421 accaacgcgc gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa
    24481 tgcgctacct gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc
    24541 cttacagtct gttaaaccgc agttttgaag taggtctggc agaagtcagc cagtatgaag
    24601 gggtcgaact gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca
    24661 atggtgcaaa acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg
    24721 gtgagcaaac gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggccttg
    24781 atcctgctca gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc
    24841 tgggcgcaac cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa
    24901 gcgaagacgt attagctgaa attgaagcgg tacatcaggt ttatacttat cctgcaccat
    24961 aaagattaac gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca
    25021 gcgccgttat tgccagcgca aatagcgcac cgaagccaat gccaatgggc acgaccggga
    25081 tgcctaccat taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc
    25141 caaggttttg tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta
    25201 gcaacgcatt gagcggaacg acaaaaaagc cccccagcac gccaatcagc atcaacaagg
    25261 cataggctgg cagcagctcg tgttgcaggg agaaaatcgg taccaccacg ccaatcaaaa
    25321 tcccggctgg catacagcgt gacacggttt ccagcgtaac cagcttcgct gccgcacctg
    25381 cgccaaccac gataccaatc gctaccatcg cgttgagata ggtcggcgta gcgttatcgg
    25441 taatgcccag cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc
    25501 cccagaataa actggtgccc accagcgaaa aacgcgtttc accattgcac catagcgagg
    25561 tgcaggcatt caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg
    25621 ccgccagttt gggaatgtag atattggcaa cgaccgcacc accgtaggcc agtgcgcatg
    25681 cggccagagc gaccaggaca tgccagtcag ccagcacacc accggctacg gaaccgagta
    25741 aaatcgccgc tatggcagaa gcttccatta aaccgttagc ttttactaac ttactacccg
    25801 tggttaattc gccgagaata ccgtatttcg ccggtgaata ggctgcagca ccaacaccca
    25861 ccagcgtata gccgagaaac ggattgatac caaagcagat actggctgcg cccagcagct
    25921 tcaggccgtt ggcaaacatc atcacccggc ctttggcgaa gctatccgcc acctgcccga
    25981 caaacggcgc caaaagaatg taagcaccta caaacaccat ttgcaggatg ggctggctcc
    26041 actccggata gaactgcgct ttcagtaacg ccagagtggc aaacagtagc gcgttatcgc
    26101 caaacgcaga gagaaactgc gccacgataa ccgctttcat ccccttcgac cacaacgaag
    26161 tgttagtgtg cactgactca ctcatcgtgt tgttccgctt cgtctaccca gcttttcaac
    26221 gtgacaaagt caggtttgcc gctgccaagt aatggcatct gtttcagata gcgaatatcg
    26281 cgcggtacag caagctccgg cacgccgtgc tcgcgggcat actgttgcaa cttatcgcgc
    26341 gtcagttcgt tatctgtggt gaaaagcacc agtgcctcgc ctttgctggc atcgctctta
    26401 atcgcagtgg catggacttt atctggcgaa acaccaagtg ccagttgttc caccatttcc
    26461 agcgacacca tttcgcctgc aattttggca aagcgttttg cgcggccctg aatctgcaca
    26521 aagccctgct cgtcaaaacg cacaatatcg ccagtgtcat accagccgcg ctccatttcg
    26581 ccgcgaacat tctcggcggt gggcacttcc agtacacctg gcttctccac ccgcagatag
    26641 ccgttcatta tgttcggccc tttcagttgc aggcgtccgc cctcttcgat accagggacc
    26701 gacaacaggc gtgcatccat tcctggtaga atacgcccta ccgtaccggg tttcgccgcc
    26761 atcggtacgt tgatagaaac gacaggcgcg cattcggtca caccgtagcc ttcaaggatg
    26821 cgcaggccaa atttatcctg ccaaagctgt ttggtacttt cttgtagttt ttctgcgcct
    26881 gccaccacat agcgtagacg atagaagtca tacgggttgg cgaaacgcgc gtagtgaccg
    26941 aggaaagtcg aggtaccgaa caacacggtg caactgcggt catacaccaa gtccggcaca
    27001 atgcggtaat gcagcgggct tggataaagg aacacttctg cacctgtaag cagtggcgta
    27061 aacaggccta ccgtcagccc aaaggagtga aacagcggta atgccgacat aaagcgatcg
    27121 ttggtggtga agtcggcaat cgttttaatc tgctcgacgt tcgccagaat gcttttatgg
    27181 ctatggacga cgccttttgg atggccttca gaaccggagg taaagaggat cagcgcctct
    27241 tcttccggct gctgttttac ctgcgccagg cgcggcatca gcaaatgagc gaagatccat
    27301 actttgtcgg cagtggtgac atctgctttt aaatcttcca gatagaccca gcgcacctga
    27361 gtaagttgct ccggcagatg ccagagtttg cctttatcga gaaactggcg ggaagtgaag
    27421 atggttttga tttcagccgc cgtaatagca ctggtcagcc cttttacccc ggcagtgtag
    27481 ttcatcattg cgggaatgcg gcgacgcgcg atggccccaa aaatcactgc cgcactgatg
    27541 cctgcattgg gcagcattaa gccgatgcgt tcgccttcaa cactgtattt ttcaaggatg
    27601 cgtccaacaa acagcgtttt cgtaagcaat ttgcgatagg agtctggggt aaagttgacg
    27661 tcttcgacac atttcttccc ggctccgaag cggtacattg cactcagtaa agattcgtac
    27721 agagtttcac gcgggcgcac cgccatacgc gcttccatca ttatttgatg cagcatttcg
    27781 ccagcgattt tgcgacggtc acgggcacgc ggcgcatccg gcatcgccac ctgcgttgga
    27841 ggcaaaatat gcagagtaat ttgcgggaac aagcgacgtt taaccagacc tttcaggcgg
    27901 ctgaagtgcg taagttccgc cccttcaata cgcacaggaa taaccgttgc accagacttc
    27961 gccgcgacaa aacccgcgcc atcgtagatt ttcatcagcg agcctgtagt ggtgatgcgt
    28021 ccttcaggga aaatcaccac tggtcggccc tgttccacca gacgtaccag atgtttaata
    28081 gccataggtt gcgtcgggtc gagaggaaca aagtcgataa atgatttcag ccaacgcata
    28141 taccactgtt ggcttattga ggtgtaaacg gcaaacactg gacgcacagg taaaaacagt
    28201 ccaagcaaaa tgccatcaat aaaagagacg tgattaggcg taattagtac gcgctcgccc
    28261 ttcagtgcct gggtgtcacc cgtaacgcga acgcgataca aaacacggca caaatttcga
    28321 aaaaagctaa aaagcattcc aactcccttt gctctgattc agtaaaagcg aatggaggga
    28381 gattacacga gataaagaac gcgagcgaca gtaaattagg tgcgaaaaaa aacctgcgca
    28441 tccgcgcagg ttggtgcaag agacagggta cgaagagcgt accgaataat ctcaccaatc
    28501 aatacctctg ggatcttgat tgtggtctgc acgacgactc ttcgccagcg agaaaacgca
    28561 aaggaatgaa gggaaatgca acgaggtgtg taaattgtcg gttactgtta cagattgatg
    28621 accggcaaaa aaaaacctgc gcatctgcgc aggctggtgt aattcatgtg ctcaacacga
    28681 agttgacttc acctatcaat acctctggga tcaccacttt agcaacctga agccaaacgc
    28741 caccagcggt caatcgcaac agcgtttcgc aaagtgtaac caaaggtttg caatctcttt
    28801 ttttgtctta cttatttgcg tttggctcac attcccacga tgaaaacacg ccaccccttg
    28861 aaccaacggg cgttttccgt aacactgaaa caatgtaagc gtttacccac taaggtattt
    28921 tcatggcgac cataaaggat gtagcccgac tggcaggcgt ttcagtcgcc accgtttccc
    28981 gcgtcattaa taattcaccc aaagccagcg aagcttcccg gctggctgtg catagtgcaa
    29041 tggagtctct tagctatcac ccgaacgcca acgcccgtgc gctggcgcag cagaccactg
    29101 aaacggtcgg tctgatcgtt ggtgatgttt ccgatccgtt tttcggtgca atggtgaaag
    29161 cggtcgaaca ggtggcttat cacaccggta atttcttatt gattggcaac ggttaccaca
    29221 acgaacaaaa agagcgtcag gctattgagc aactgatccg ccatcgctgt gctgcgttgg
    29281 tcgtccatgc caaaatgatc ccggatgccg atttagcctc attaatgaaa caaatgcccg
    29341 gtatggtgct gatcaaccgt atcctgcctg gctttgaaaa ccgttgtatt gctcttgacg
    29401 atcgttacgg tgcctggctg gcaacgcgtc atttaattca gcaaggtcat acccgcattg
    29461 gttatctgtg ctctaaccac tctatttctg acgccgaaga tcgtctgcaa gggtattacg
    29521 atgcccttgc tgaaagtggt attccggcca atgatcggct ggtgacattt ggcgaaccag
    29581 acgaaagcgg cggcgaacag gcaatgaccg agcttttggg acgaggaaga aatttcactg
    29641 cggtagcctg ttataacgat tcaatggcgg cgggcgcgat gggcgttctc aatgataatg
    29701 gtattgatgt accgggtgag atttcgttaa ttggctttga tgatgtgctg gtgtcacgct
    29761 atgtgcgtcc gcgcctgacc accgtgcgtt acccaatcgt aacgatggcg acccaggctg
    29821 ccgaactggc tttggcactg gcggataatc gccctctccc ggaaattact aatgtattta
    29881 gtccgacgct ggtacgtcgt cattcagtgt caactccgtc gctggaggca agtcatcatg
    29941 caaccagcga ctaaccgcag ttaaagcaat tccagcgcca gtaattcttc gatggtctgg
    30001 cgacggcgaa tcaaccgcgc ctgaccatta tcaaacagaa cttctggtaa cagcggacgg
    30061 ctattgtagt tggatgacat tgatgcgcca tatgcccctg tatcatgcag taccagataa
    30121 tcacctgcct tcacttccgg caaggcgcgg gtttcaacat ttcccccttc ctgctgggta
    30181 aagacatcgc ccgattcaca taacggcccg gcgacgacgg tttccaccgt tggcgcgtgt
    30241 tccagagaac gaccatcagc tgccagggca ctgatatggt ggtaactacc gtacattgcc
    30301 gggcgcatca gatcgttgaa cccggcatca accagcacaa agtggcggct acccatttgt
    30361 ttgacgctcc gcacctgagt aattaatacg ccagactgcg ctaccaggaa gcgacccggt
    30421 tcaatttcca gtttcacagg gtggcccaaa tggcgagcga tttgctcacg cgcggcattc
    30481 cacagaccat aataatgttc ggtatcaacc gcctcttcac cctgttgata aggaatagaa
    30541 agcccaccgc ccgcagaaat agcctgtaaa tcctgaccga attcgataac ctgacgcacc
    30601 atagcaccac acacctgctc cagatgggca taatcaacgc cagaaccaat gtgcatgtga
    30661 atgccgacta gctgcagatg atgacgttgt atcacgtcca gtgcggcggg cagatcggtg
    30721 taccagatac cgtgcttgct gttttcgcca ccggtattgg ttttttggct atgtccgtga
    30781 ccaaaccccg gattaacgcg cagccatacc cgatgccctg gcgaaacctg gcccagttgg
    30841 tcgagcgtat caacagaacc cgcattcacc ggaatttgca attcactgac gcgttcaagc
    30901 gtcgcctgat cgataacatc tgccgtaaaa acaatatcat cggggtgcgt ttgcggattg
    30961 taacccgccg ccaacgcacg ctctatttcg cctaacgaga cggaatccac tttcacgccc
    31021 tgctcacgca ttaagcgcaa aatatgaata ttggaacagg ctttctgtgc aaagcgcacc
    31081 acttcaaact gtttcagcgc tgcaatctgc cgacgaataa tttgcgcatc gtagacccac
    31141 accgggcagc caaattcagc gggcaaacgc agcagatttt cggcggtgag atcggtatcg
    31201 gtgctgaaca gtgaatgtgg cataacaaac tccagatgag tgctttttta tgattacgcc
    31261 acatcataaa aagaataaaa aatatcgatt tatgtcgagt ctatgcaaaa atgatatgga
    31321 ttaccggatt gcgagagagc gctaatggcc gccgttaact tacgtcatat tgaaattttt
    31381 catgcggtaa tgaccgccgg aagcctgact gaggcagcac acctgctaca cacctcacag
    31441 ccaaccgtca gccgcgaact ggcgcgcttt gagaaggtga tcgggctgaa attgtttgag
    31501 cgcgtacgtg gacgattaca tcctaccgtg caaggactgc gtctgtttga agaagtgcaa
    31561 cgatcctggt acggactgga tcgcattgtc agcgccgcag aaagtctgcg cgagtttcgc
    31621 cagggagaac tgtctattgc ctgcctgccg gtcttttcgc aatctttttt accgcagctc
    31681 ctgcaaccct ttctggcacg ttatcccgat gtcagcttaa atatcgtgcc ccaggaatca
    31741 ccgctacttg aagagtggct ctcggcccag cgtcatgatt taggactcac tgagacactc
    31801 catacgccta cgggaacaga acgtaccgaa ttactctctt tagatgaagt gtgtgtgtta
    31861 cctccgggtc atccgctggc ggtaaaaaag gtattaacgc cggatgattt tcagggtgag
    31921 aattacatca gcctttcccg tactgacagc tatcgccagt tgctggatca gctatttact
    31981 gaacatcagg ttaaacgacg catgatcgta gaaacccaca gcgccgcgtc agtctgcgca
    32041 atggtacggg cgggggtagg tatttcggtg gttaacccgc tcaccgcact agattatgcg
    32101 gcaagcggtt tagtggtgcg gcggttcagt attgcggttc cgttcaccgt cagcctgatc
    32161 cgccccctgc accgcccgtc atcagcgctg gttcaggcgt ttagtgggca tttacaagcg
    32221 gggttaccga aactggtcac ttctcttgac gctattttgt cgtcagctac gacagcataa
    32281 aagcgacagc atcctcggca tggatcgccg cggtatcaaa cacaggcaaa acactgcgct
    32341 cttctggcac cagtaaacca atttctgtgc agccaaaaat gacgccctgt gcgccctgtt
    32401 ctgcaaggcg agcaatcact tgcgcataat aagcgcgtga cgcttcggta aattgcccca
    32461 gacacagttc ttcaaaaata atctgattaa ttttcgcccg ttcatccgct tcaggaataa
    32521 gacaattgat ggaaaattgt tccgtcagcc gcccgcgata aaaatcctgt tccatggtgt
    32581 aacgcgtacc cagcagcgcc acacgagtca ttccggcccc ggtaattgca cgtccggtgg
    32641 catccgcaat gtgtaagaaa ggcagagtgc aacgtgactc aatggcatcc gccactttat
    32701 gcatcgtatt ggtacataac acaatacctt ctgcgcccgc ccgctgtaag ccaagcgccg
    32761 cctcagccag aatgtccccg gttttatccc attccccgcg acgctggcac tcttctattt
    32821 catgaaaatc gacgctatgt agcagcattt gcgcagagtg aagcccacca agccgctgtt
    32881 taatgccttc atttatcaga cgatagtaag gaatggtgga ttcccagctc attcctccca
    32941 gcaaaccaat tgttttcatt cctctctcct gatgtgtgtt accgcaacaa ggctgaataa
    33001 ggaacgagac aaacgcctca acggccaggt gcccaatctc tattaacgaa aaaagggccg
    33061 gatgtacagc acatccggcc cgtgaaatca gacgccgata tttctcaact tctcgcctgc
    33121 catcagtttg cgttcgatat gttccagcgt gacatttttg gtttccggaa tgagccagaa
    33181 agtaataccc acaaacgcaa tgttcagcgc agtgtagagc cagaacgtac cggcagcgcc
    33241 aatgctatca agcagtgtca ggaaggccgc gccgataatc atattcgaca cccagtttgt
    33301 cgtcgtcgaa caggtaatac cgaaatcgcg gcatttcagc ggctgaattt cagagcacag
    33361 gatccacacc actggcgcgg cgctcatcgc ataaccggca atacacatca tcgtcatgcc
    33421 aacagagagc caggacaagc cactggaagc cgtaccgtta tcaaactgca tcaggcaata
    33481 gcccagcacc agagtgccta acgccatcac gctgaaacca attttcagag ccggtttacg
    33541 acctgcttta tctaccgtaa agaccgcaat gaaggtcgcg aacataaagg tcagtcccac
    33601 gaccagagtc gcaatcatct gttgttctgt ggtcgtaaag cccgccattt tgaagatacg
    33661 cggcgcgtag tacatgatga tgttcatacc ggtaaactgc tgcatcgcct gcaacaacat
    33721 accgagaaac acagcacgac ggacgttacg gttgatctta aacagtgccc aaccgccctg
    33781 ttttaacttc aggctttcac gaatttcgtt gagttcttct cgcgcttttt ccgacgtatc
    33841 gcgcagcata cgcaacactt cttccgcctc aatatgacgc cccttttccg ccagccagcg
    33901 cgggctattt ggcaggaaga ccaccagaat aatcagcaga actgctggta aagcaagaac
    33961 ccccaacatt gcgcgccagt taccgctata actgaacgct gtatcggata aaaacgccag
    34021 cacgatgccg agtgtgacca tcaactggta catactgatc atcttaccgc gaacgttttc
    34081 acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga cagcaatgcc
    34141 cagcaccaca cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg accctataga
    34201 accgagtaca aacaggatgg cccccgccat caggctgtat ttacgcccca ggcggaacga
    34261 cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac taaccaccca
    34321 ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg ctccggcgat
    34381 tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg aaacaaacat
    34441 attcatacgc cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg tattgatagt
    34501 aaccattttt tcctgccagc agagagtaag acattgtgaa aaaatacgtg aacaattcac
    34561 acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca tgccgttatg
    34621 tgatggatat tccaatttta aaattaagtt gaattattga gattattatt aacctcctaa
    34681 ttttaaagca gataaaattc ataaagttca ttaattgata attgatatgg attatttcat
    34741 aaccatgata tggattatga tgatctagag atataaaaaa ccctgccatg cggcagggtc
    34801 ataaaagtaa gaggaatgaa ttaacgcgcc agccaaccgc catccacagc aatggcataa
    34861 ccatttacat aatctgaagc gctggaggca agaaacacta ccggccccat caggtcactc
    34921 ggcagtcccc aacggccagc tggaatgcga tcgagaattt ccgcgctacg ttgttcatct
    34981 gcccgcagtt gttgggtatt gttggtcgcc atgtaacccg gagctatcgc attaacatta
    35041 atgttgtgtt tagcccattc gtttgccatc aatcgcgtca cacccatcac gccgcttttt
    35101 gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga cgcgatattg
    35161 ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg agacatgaag
    35221 aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct gaactcgaga
    35281 gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaattca
    35341 gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc ggcggtcagg
    35401 cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg ttcaacgatg
    35461 ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc ctggcccagc
    35521 ccagtatcac aaccagtgac gaccgcaact ttaccttgga gagaaaatgc acttaaaatc
    35581 ataacaacac cttattcgtt tatgcccaca actagcgcaa atctttaacg cccacatggt
    35641 ccatatcatt aaagacctgg ttttcaccga ccatgcccca gataaaggta taagctttgg
    35701 ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca
    35761 caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca
    35821 tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc atggtgttcc
    35881 acaagttacc tggagccagc tccgtcagcc ccatactcaa ttgacaggtt tccagtacat
    35941 ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg cctaacgtga
    36001 ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc
    36061 aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca acttcttttg
    36121 cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga ccatcgacag
    36181 taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca aggaaatagc
    36241 ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt atcggcataa
    36301 tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa
    36361 ataccttttc aaccaaaaat tcattgcgca gcccttgggt atccagcgtt tttgcgtgcg
    36421 caccgtggat gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa
    36481 tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata tgcccatggg
    36541 ctagggtttt caattaaaaa taaaacaatg ttttattttt aatttaagga ttaaaaaaag
    36601 tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc agaaaatgta
    36661 tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt tatcggtaag
    36721 gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgccctg gcccccgccg
    36781 atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg aaccagagaa
    36841 accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc gccaccattg
    36901 acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc ctgtgcagca
    36961 aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac acgctccagg
    37021 caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac accaacgctg
    37081 gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc
    37141 atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc ggtcacagaa
    37201 ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc gtcagtgcgt
    37261 ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat taccgggacg
    37321 atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt
    37381 gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt ttcggcggtg
    37441 acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag actgtcaacc
    37501 aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg tggtgcgcgg
    37561 ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg tatcgcctga
    37621 gtagccagat gcagtgcttt aagaccggaa ccgcaaacgt cattaatcgt gattgcagaa
    37681 acgctgttag gaagaccacc tttaatagcc gattgccttg ccggattctg ccctgcccct
    37741 gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac gccggtacgt
    37801 tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc cgctaacgca
    37861 ccacgaaagc agccgatagg tgtccgtaac gccccgacaa tcacaacgtc tttcatcaca
    37921 acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtca tcttaattgt
    37981 ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca gagtcataac
    38041 tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagatagg gcgttaatct
    38101 cccatactta acttggttta tggtaaattg ccctccattt tgtttaattt gtagatgata
    38161 cgttcagata atgtctaata tttggtctaa agaagaaact ctgtgaagtt tcgcgctcta
    38221 tggcacagcc gttgatgcag gcacgctatt ccttcctatt cagttaggtt cggcaggggc
    38281 tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattggc cacataaagc
    38341 cttatgccag ttcatcctct catcaaaaac atcagcaggt gaagggataa cgggcgcggt
    38401 aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact tcatcgcctt
    38461 ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg aacagctggc
    38521 aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg ttgtattaat
    38581 tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg gatttttggt
    38641 attcccattg attgcctatt tcttatttct ttccatttac cttgtcggta gttggcaacc
    38701 tgatctatta acaacccagg tagagttcaa tcagaatacc cttcaccaga tatggatatc
    38761 gattcccgtg atggttttcg cctttagcca tacgcccatt atttctacgt ttgccataga
    38821 cagacgtgaa aaatatggcg aacacgctat ggataaatgc aaaaaaatta tgaaagtcgc
    38881 ttatctcatc atctgcataa gtgtactgtt ctttgtcttt agctgcctgc tttctattcc
    38941 atcttcgtat attgaagcgg ctaaagaaga aggggtcacc attttatcgg cgctttctat
    39001 gctgccgaac gccccagcat ggttgtcaat ttccgggatt attgtcgcag tagttgcgat
    39061 gtcgaaatca ttcctgggta cgtactttgg cgttattgaa ggtgccacag aggtcgtcaa
    39121 aacaacacta cagcaggttg gtgtaaagaa aagtcgtgca tttaaccacg cactatcaat
    39181 tatgttggta tcgctgatta ccttcattgt ttgttgcatt aacccgaacg cgatttcgat
    39241 gatttacgcg atcagcggcc cgctcattgc catgatactt ttcatcatgc ctacgctgtc
    39301 aacgtatctc atcccggcgc ttaaaccctg gcgttccatc ggaaatctga ttacgctgat
    39361 cgtgggtatc ctgtgcgtat cggtaatgtt ctttagctaa tatccgttgt ggatggttca
    39421 aaattgctac aattttgaac cattccattt ttcataatat tcatttgtta ttattccgcc
    39481 ctatcacttc tctcgtcata tcctagaatt atcagttagt taaacacaca aagagatctg
    39541 aacatcttaa ataagactat ttaagatgca taacttagat tcgcaagata tcttctggtc
    39601 attcaaaaac aatttgcgat atagatattc tcataccatg agatagttat ctggaccacg
    39661 ttcgaccatg caagattaaa tcagtgaaac ataatcatat ttaataacga gaaatgcatt
    39721 tttaaatatc ttaatcagaa ttatagcaaa tacagattaa tcgcaatttt tcccagcaaa
    39781 attacaaaat caaggtgtat tgacatcatc aatcaccaat gaaattatgc aattatatac
    39841 ggatagggag gttcttaaca tggggctttg tagtcgttat aaaagtctta catgcaatag
    39901 ttgctcaatg cattgccaaa taatgccaga agagtcaccg cgtttgcagt actgcgcaaa
    39961 ctcgtgtttt gatcttaccc agcaatagtg gacacgcggc taagtgagta aactctcagt
    40021 cagaggtgac tcacatgaca aaaacagtat caaccagtaa aaaaccccgt aaacagcatt
    40081 cgcctgaatt tcgcagtgaa gccctgaagc ttgctgaacg catcggtgtt actgccgcag
    40141 cccgtgaact cagcctg
//
LOCUS       DAJVPP010000027        40092 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVPP010000027 DAJVPP010000000
VERSION     DAJVPP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.027"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(222..2255)
                     /gene="metG"
                     /locus_tag="OQM34_001396"
     CDS             complement(222..2255)
                     /gene="metG"
                     /locus_tag="OQM34_001396"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS1451906.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2387..3496
                     /gene="apbC"
                     /locus_tag="OQM34_001397"
     CDS             2387..3496
                     /gene="apbC"
                     /locus_tag="OQM34_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1451907.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3758..4039
                     /locus_tag="OQM34_001398"
     CDS             3758..4039
                     /locus_tag="OQM34_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS1451908.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4332..4856
                     /locus_tag="OQM34_001399"
     CDS             4332..4856
                     /locus_tag="OQM34_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1451909.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKVIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            4937..5611
                     /locus_tag="OQM34_001400"
     CDS             4937..5611
                     /locus_tag="OQM34_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1451910.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5627..8107
                     /locus_tag="OQM34_001401"
     CDS             5627..8107
                     /locus_tag="OQM34_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1451911.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8123..9156
                     /locus_tag="OQM34_001402"
                     /pseudo
     CDS             8123..9156
                     /locus_tag="OQM34_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9238..9576)
                     /gene="rcnB"
                     /locus_tag="OQM34_001403"
     CDS             complement(9238..9576)
                     /gene="rcnB"
                     /locus_tag="OQM34_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS1451912.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9795..10634)
                     /gene="rcnA"
                     /locus_tag="OQM34_001404"
                     /pseudo
     CDS             complement(9795..10634)
                     /gene="rcnA"
                     /locus_tag="OQM34_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10756..11028
                     /gene="rcnR"
                     /locus_tag="OQM34_001405"
                     /pseudo
     CDS             10756..11028
                     /gene="rcnR"
                     /locus_tag="OQM34_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      11120..11216
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11251..12039
                     /gene="thiM"
                     /locus_tag="OQM34_001406"
     CDS             11251..12039
                     /gene="thiM"
                     /locus_tag="OQM34_001406"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1451913.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12036..12836
                     /gene="thiD"
                     /locus_tag="OQM34_001407"
     CDS             12036..12836
                     /gene="thiD"
                     /locus_tag="OQM34_001407"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1451914.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            12901..13722
                     /locus_tag="OQM34_001408"
     CDS             12901..13722
                     /locus_tag="OQM34_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1451915.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13770..14516
                     /locus_tag="OQM34_001409"
     CDS             13770..14516
                     /locus_tag="OQM34_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1451916.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14490..15455)
                     /locus_tag="OQM34_001410"
     CDS             complement(14490..15455)
                     /locus_tag="OQM34_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCS1451917.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15452..16455)
                     /locus_tag="OQM34_001411"
                     /pseudo
     CDS             complement(15452..16455)
                     /locus_tag="OQM34_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16452..17729)
                     /locus_tag="OQM34_001412"
     CDS             complement(16452..17729)
                     /locus_tag="OQM34_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1451918.1"
                     /translation="MKTTAKLSLMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            17986..19038
                     /gene="fbaB"
                     /locus_tag="OQM34_001413"
     CDS             17986..19038
                     /gene="fbaB"
                     /locus_tag="OQM34_001413"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1451919.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19346..20200
                     /gene="gatY"
                     /locus_tag="OQM34_001414"
     CDS             19346..20200
                     /gene="gatY"
                     /locus_tag="OQM34_001414"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1451920.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20229..21491
                     /gene="gatZ"
                     /locus_tag="OQM34_001415"
     CDS             20229..21491
                     /gene="gatZ"
                     /locus_tag="OQM34_001415"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCS1451921.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21501..21953
                     /gene="gatA"
                     /locus_tag="OQM34_001416"
     CDS             21501..21953
                     /gene="gatA"
                     /locus_tag="OQM34_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1451922.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21984..22268
                     /gene="gatB"
                     /locus_tag="OQM34_001417"
     CDS             21984..22268
                     /gene="gatB"
                     /locus_tag="OQM34_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1451923.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22272..23627
                     /locus_tag="OQM34_001418"
     CDS             22272..23627
                     /locus_tag="OQM34_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS1451924.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23675..24715
                     /gene="gatD"
                     /locus_tag="OQM34_001419"
     CDS             23675..24715
                     /gene="gatD"
                     /locus_tag="OQM34_001419"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1451925.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24821..25594
                     /locus_tag="OQM34_001420"
     CDS             24821..25594
                     /locus_tag="OQM34_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1451926.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25676..26575)
                     /gene="yegS"
                     /locus_tag="OQM34_001421"
     CDS             complement(25676..26575)
                     /gene="yegS"
                     /locus_tag="OQM34_001421"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCS1451927.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            26981..27298
                     /locus_tag="OQM34_001422"
     CDS             26981..27298
                     /locus_tag="OQM34_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451928.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27640..29001)
                     /gene="yegQ"
                     /locus_tag="OQM34_001423"
     CDS             complement(27640..29001)
                     /gene="yegQ"
                     /locus_tag="OQM34_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1451929.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29148..29479)
                     /locus_tag="OQM34_001424"
                     /pseudo
     CDS             complement(29148..29479)
                     /locus_tag="OQM34_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(29670..30392)
                     /gene="baeR"
                     /locus_tag="OQM34_001425"
     CDS             complement(29670..30392)
                     /gene="baeR"
                     /locus_tag="OQM34_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS1451930.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(30389..31792)
                     /gene="baeS"
                     /locus_tag="OQM34_001426"
     CDS             complement(30389..31792)
                     /gene="baeS"
                     /locus_tag="OQM34_001426"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1451931.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(31789..33204)
                     /locus_tag="OQM34_001427"
     CDS             complement(31789..33204)
                     /locus_tag="OQM34_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1451932.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(33205..36270)
                     /gene="mdtC"
                     /locus_tag="OQM34_001428"
     CDS             complement(33205..36270)
                     /gene="mdtC"
                     /locus_tag="OQM34_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1451933.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(36271..39393)
                     /gene="mdtB"
                     /locus_tag="OQM34_001429"
                     /pseudo
     CDS             complement(36271..39393)
                     /gene="mdtB"
                     /locus_tag="OQM34_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(39393..>40092)
                     /gene="mdtA"
                     /locus_tag="OQM34_001430"
     CDS             complement(39393..>40092)
                     /gene="mdtA"
                     /locus_tag="OQM34_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCS1451934.1"
                     /translation="ASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVV
                     ITQTHPIDLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDA
                     TTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNS
                     ENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEE
                     KATSREYAKKGARS"
BASE COUNT        10131 a         9823 c        10369 g         9769 t
ORIGIN      
        1 actgatatgc aactcgtttc cctgttgctc caccgtccag aatttatgtg atttttcgtc
       61 ctgatagata aagtgtctca ttctgcatcc ctgtaatttt atccagttga atgcagatgc
      121 taccagtatt tatacgggtt agagagagac aaaatcgcaa gaaaacaact ttaggaattt
      181 ataaagaaaa ggcgctgtcg atgcagcgcc ttgaaggggg attatttcac ctgatgaccc
      241 ggtttagcac cggcatccgg gcttagcagg aaaatatctt tcccgccagg accggcagcc
      301 atcaccatgc cttcagagat accgaagcgc attttacgtg gtgccaggtt agccaccata
      361 atggtgtgac gaccaatcag tgcctgcgga tccgggtaag cagaacgaat accggagaag
      421 acattgcgtt tttcaccgcc gagatccagc gtcaggcgca gcagtttgtc agaaccttca
      481 acaaactctg cgttttcgat cagcgccacg cgcaggtcaa ctttggcgaa gtcgtcaaag
      541 gtgatggttt cctgaatcgg atcatctgcc agtgggccag taactggcgc ggtagtggct
      601 ttcacttctt ctttagaggc ttccaccaga gcttcaacct gtttcatatc gatgcggtta
      661 tacagcgcct tgaacggatt cactttgtgg cccagcagcg gttgctggat accatcccag
      721 gtcagttccg tattgaggaa ggcttctgca cgctcggtca gtttcggcag taccggcttc
      781 aggtaagtca tcagcacgcg gaacaggttg atgcccattg agcaaatcgc ctgcaggtca
      841 gcatcgcgac cttcctgttt tgcgactacc cacggagcct gctcgtcgac atagcggtta
      901 gccaggtcag ccagtgccat gatttcgcgc actgctttac caaattcacg gctttcccac
      961 gcttcaccaa tcacttcagc ggcatcggta aaggttttgt acagctcagg gtcagccagc
     1021 tcgcttgcca gcacaccgtc aaaacgcttg ttgataaagc ccgcattacg ggaggccagg
     1081 ttaaccactt tgttcacgat atcggcattc acacgctgaa cgaaatcttc caggttgaga
     1141 tcgatatcat caatgcgcga agagagtttc gcagtgtagt agtaacgcag gctgtcagcg
     1201 tcaaaatgat tcagccaggt gctggcttta ataaaggtgc cgcgagactt ggacatcttc
     1261 gcgccgttca ccgtcacata gccatgaaca aacaggttgg tcggcttgcg gaagttgctg
     1321 ccttccagca tggcaggcca gaacaggctg tggaagtaaa cgatatcttt accgatgaag
     1381 tggtacagct cggcggtgga gtctttcttc cagtattcat cgaagcttac gctgtcgccg
     1441 cgcttgtcgc acagattctt gaaagaaccc atgtagccaa tcggtgcgtc cagccagacg
     1501 tagaaatatt tgcccggtgc gttcggaatt tcaaaaccaa agtatggcgc gtcgcgggag
     1561 atatcccact gttgcaggcc ggattcaaac cactcctgca ttttgtttgc cacctgctcc
     1621 tgcaacgcac cgctgcgggt ccatgcctgc agcatttcgc tgaaagaggg cagatcaaag
     1681 aagaagtgtt cagaatcacg catcactggt gtagcaccag aaaccaccga tttcggctcg
     1741 atcaattcag tcgggctgta ggtcgcgccg cagacttcgc agttatcgcc gtattgatcc
     1801 ggggatttac atttcgggca ggtgcctttc acaaaacggt ccggcaggaa catgcctttt
     1861 tccggatcgt acagctgaga gatggtgcgg tttttaataa aaccgttttc tttcaggcga
     1921 gagtagataa gttctgacaa ctggcggttc tcttcgctgt gcgtcgagtg atagttgtca
     1981 tagctgatgt taaagcctgc gaaatcagtc tgatgctcct gactcatttc gccaatcatc
     2041 tgctccgggg tgataccgag ctgctgagct ttcagcatga tcggtgtacc gtgggcatcg
     2101 tcggcgcaga tgaagttgac ctcgtggccg cgcattcgct ggtaacggac ccagacatca
     2161 gcctggatgt gctccagcat atggccgagg tggattgagc cgttagcgta cggcagtgcg
     2221 cacgtcacca gaattttctt cgcgacttga gtcatagtag gcattacttc ttaatagtga
     2281 aaaggggtta gatagtacca aatgggaaaa tgttaagtaa gtacttctct atattcttca
     2341 ggtaaactat cagctgatgg tttatctata caaaaaagga gtcgggatga acgaacaatc
     2401 ccaggccaaa tcaccagaag ccttgcgcgc aatggtcgcc gggacactgg ccaattttca
     2461 gcacccaacc ctgaagcata acctcaccac gctgaaagcg ttgcaccatg tcgcctggat
     2521 ggacgacacg ttgcatgtgg aactggttat gccgtttgtc tggcatagcg cgttcgaaga
     2581 gttaaaagag caatgtagcg ccgaactgct gcgcatcacc ggggcgaaag ctatcgactg
     2641 gaaactgtcg cataacatag ccactctgaa acgcgtgaag aatcagccgg gaattaacgg
     2701 tgtgaagaac attatcgcgg tgagttccgg taaaggcggc gtgggtaaat cgtccacggc
     2761 ggtaaacctg gcgctggcac tggcggcgga aggggcgaaa gtcggcattt tggacgccga
     2821 tatctacggc ccatcaattc ccaccatgct gggcgcagaa aatcagcgtc caacttcgcc
     2881 ggacggtacc cacatggcac cgattatgtc tcatggcctg gcaaccaatt caattggtta
     2941 tctggtcact gacgacaatg cgatggtgtg gcgtggaccg atggcgagca aggcgttaat
     3001 gcagatgttg caggaaacct tgtggccgga tctcgactat ctggtgcttg atatgccgcc
     3061 aggcaccggt gacattcaat tgacgctggc gcaaaacatt ccagtaactg gtgcagtcgt
     3121 ggtcaccacc ccgcaggaca tcgcgttgat cgatgcgaaa aaaggcattg tgatgttcga
     3181 gaaagtcgag gttccggtgc tcggtattgt cgagaacatg agtgtgcata tttgcagtaa
     3241 ctgtggtcac catgaaccga ttttcggtac cggtggtgca gagaaactgg ctgaaaaata
     3301 ccatacacag ctgctggggc agatgccgtt acacatctcc ttacgtgagg atctcgataa
     3361 aggcacgcca acggttatca gccgtccgga gagcgaattt accgctatct accgccagct
     3421 ggctgaccgc gttgcagctc agctctactg gcagggtgaa gtcattcctg gcgagatttc
     3481 cttccgcgcg gtctaacgcc tattaaccat tctggttatt cttcatacac cgcagagatg
     3541 ttatattgat gcggtgtatt tgtttttgcc gcattaatga acacttattt aatactgcga
     3601 atattctgcg gaccttcgtc cgaaactcta caaagtcgag tgatcgtaca ggcaatgctt
     3661 cattatcatc cttcgtgctg tgtagtggat gtacagctat agtttgttat gcactcattc
     3721 tatccaggca taacctttac tacagggata acgttcaatg aataagtatt ggctgtcagg
     3781 aattattttt cttgcctatg gattggcttc tcccgcagtc tcttcagaga ctgccacatt
     3841 aacgattaat ggcaggatca gtcccccaac atgcagtatg gctatggtta atagtcaacc
     3901 acaacagcat tgtggtcagt taacgtataa tgttgacacc cgtcatcagg tttcttcgcc
     3961 cgtcaaaggt gttacgacag aagtggttgt tgcaggtagc gatagcaaac gtcgaatcgt
     4021 gttaaatcgc tacgattaat ttggcaacct gattttaatt gcatttagaa agacgaaatc
     4081 atttatataa atgatttcgt cttttttatt gatatcaaca ataccattta cataaatcta
     4141 cattaattaa tagatgtgat aaatatttaa tattcttaag tatttgttgt atttatattg
     4201 cattggttaa tttgttttaa atcaataaaa caatatgttt acattgcggc agcttttttg
     4261 atgtgagaaa tttgtttcca agagatacag actcttaaca accaaaaata aattttatta
     4321 aggaacatat tatgaaacgt tcaattattg ctgccgctgt cttttcttct ttttttatga
     4381 gcgctggagt atttgctgca gacgttgata ccggaacatt aaccattaaa ggaaatattg
     4441 cagaatctcc gtgtaaattc gaagcgggtg gtgattcagt aagtattaat atgccgactg
     4501 taccaaccac tgtctttgaa ggtaaagcta aatattctac ctatgatgat gcagtcggtg
     4561 taaccagcag catgttaaaa attagctgcc cgaaagaagt tgctggtgta aaactctcgt
     4621 tgatcacaaa cgacaaaata accggtaacg ataaggtgat agccagtagc aacgataccg
     4681 taggtgataa cagcgatgtc ctagatgttt ctgcaccttt taacattgag agttataaaa
     4741 cagcggaagg tcaatatgct attccgttta aagcaaaata cctgaaactg acagataact
     4801 cagtgcaatc aggtgatgtg ttatcttctc tggttatgcg tgtggcgcag gattaataaa
     4861 taaaattgca agtttctcca ggtaataaac atggccgcta tccccatggc ggctttttaa
     4921 tttaagaggc attaagatga aaggattatt atctttactc attttttcta tggtccttcc
     4981 tgcacatgcc ggaattgtta tctacgggac gcgcattatt tacccggcag aaaataaaga
     5041 agttatggtg cagttgatga accagggaaa tcgttcttcg ctgctgcagg cgtggattga
     5101 tgatggcgat acgtcgttac caccagaaaa aattcaggtt cctttcatgt taacgccacc
     5161 agtggcaaaa ataggggcaa attccgggca gcaagtaaaa atcaaaatta tgccgaataa
     5221 actgcccact aataaagaaa gcatttttta tctgaatgtt ctgtatattc caccaaatag
     5281 tccagagcaa gaaggcaaga atgcactgaa gtttgcgatg caaaacagaa ttaagttgtt
     5341 ttaccggcca gcgggtattg ctccggtaaa taaagcgacc tttaaaaagt tactggtaaa
     5401 tcgcagtggc aatggattgg tgataaaaaa tgactcagct aattgggtga cgatttcgga
     5461 tgtcaaagct aataatgtta aagtcaatta tgaaactatt atgattgccc ccttagaaag
     5521 tcagagtgtt aatatcaaaa gtaataatgc aaataactgg tatctgacca ttatcaatga
     5581 ccacggcaac tatattagtg acaaaattta attgcaggag ctgcctatgt tgagaatgac
     5641 cccacttgca tcagcaatcg tagcgttatt gctcggcatt gaagcttatg cagctgaaga
     5701 aacctttgat acccatttta tgataggtgg aatgaaagac cagcaggttg caaatattcg
     5761 tcttgatgat aatcaaccct taccggggca gtatgacatc gatatttatg tcaataagca
     5821 atggcgcggg aaatatgaga ttattgttaa agacaacccg caagaaacat gtttatcaag
     5881 agaagttatc aagcggttag gcattaatag cgataacttc gccagcggta agcaatgttt
     5941 aacatttgag caacttgttc agggtgggag ctatagctgg gatatcgggg tttttcgtct
     6001 cgatttcagt gtcccgcagg cttgggtgga agaactggaa agtggctatg ttccaccgga
     6061 aaactgggag cggggtatta atgcgtttta tacttcttat tatgtgagtc agtattacag
     6121 cgactataaa gcgtcgggta acaacaagag tacatatgta cgttttaaca gcgggttaaa
     6181 tttactggag tggcaactgc attctgatgc cagtttcagt aaaacaaata acaatccagg
     6241 ggtgtggaaa agcaataccc tgtatctgga acgtggattt gcccaatttc tcggcacgct
     6301 tcgcgtgggt gatatgtaca catcaagcga tatttttgat tctgttcgct tcagcggtgt
     6361 gcggttgttt cgtgatatgc agatgttgcc taactcgaaa caaaatttta cgccacgggt
     6421 gcaggggatt gctcagagta acgcgctggt aactattgaa cagaatggtt ttgtggttta
     6481 tcagaaagag gttcctcctg gcccgtttgc gattacagat ttgcagttgg ccggtggtgg
     6541 agcagatctt gatgttagcg tgaaagaggc ggacggctcg gtaaccacct atctggtgcc
     6601 ttatgcagcg gtgccaaata tgctgcaacc cggcgtgtcg aaatatgatt ttgcggcggg
     6661 ccgtagccat attgaagggg cgagcaagca aagtgatttt gtccaggcag gttatcagta
     6721 tggttttaat aacttattga cgctgtatgg cggcacgatg gttgctaata attactatgc
     6781 gtttaccctc ggaacgggtt ggaacacgcg cattggcgca atttccgtcg atgccacgaa
     6841 gtcgcatagt aaacaagaca acggcgatgt gtttgacggg caaagttatc aaattgccta
     6901 caacaaattt gtgagccaaa cgtcgacgcg ttttggtctg gcggcctggc gttattcgtc
     6961 gcgtgattac cggacattta acgatcacgt ttgggcaaac aataaagata attatcgccg
     7021 tgatgaaaac gatatctatg acattgccga ttattaccag aacgattttg gccgtaaaaa
     7081 tagcttttct gccaatatga gccagtcatt gccagaaggc tggggttctg tgtccttaag
     7141 tacgttatgg cgagattact gggggcgtag cggcagcagt aaggattatc agttgagtta
     7201 ttccaataac tggcggcgga taagctatac cctcgcggca agccaggcgt atggcgagaa
     7261 tcatcatgaa gagaaacgtt ttaatatttt tatatcaatt ccctgtgatt ggggtgatga
     7321 cgttacgacg cctcgtcggc aaatatatat gtctaactca acgacgtttg atgatcaggg
     7381 ttttgcctca aataatacgg gattatcagg aacagtaggg agtcgggatc agttcaatta
     7441 tggtgtcaac ctgagtcatc aacatcaggg aaatgaaacg acagctgggg cgaatttgac
     7501 ctggaacgcg ccggttgcga cagtgaatgg cagttatagt cagtcgagta cttatcgaca
     7561 gactggagcc agtgtttcag ggggcattgt cgcctggtcg ggtggcgtta atctggcgaa
     7621 ccgtctttcc gaaacgtttg ctgtgatgaa tgcgccagga attaaagatg cttatgtcaa
     7681 tgggcaaaaa tatcgcacaa caaaccgtaa tggagtggtg gtatacgacg gaatgacacc
     7741 ttatcgggaa aatcacctga tgctggatgt gtcgcaaagc gatagcgaag cagaattacg
     7801 tggcaaccgg aaaattgccg ccccttatcg cggcgcggtt gtactggtta attttgatac
     7861 cgatcagcgc aagccctggt ttataaaagc gttaagagcg gatggtcaac cattaacgtt
     7921 tggttatgaa gtcaatgata tccatggtca taatattggc gttgtcggcc agggaagcca
     7981 gttatttatt cgcaccaatg aaataccgcc atcggttaat gtagcaattg ataagcaaca
     8041 aggactttca tgcacaatca ccttcggtaa agagattgat gaaagtagaa attatatttg
     8101 ccagtaatag tcaggtggtt ctatggaaat tcgcataatg ctatttatat taatgatgat
     8161 ggttatgcct gtgagctatg cggcatgtta tagtgagtta tctgttcagc acaatttggt
     8221 tgttcagggg aattttgcac ttactcaaac acaaatagcg acatatgagc ataattttaa
     8281 tgattcgtca tgcgtgagta caaatactat cacccctatg agcccatcgg atattattgt
     8341 tggactttat aacgatacca ttaaattaaa tttacatttt gaatggacca ataaaaacaa
     8401 catcacgttg tcaaataatc agagcagttt caccagtggt tattcggtaa cggtgacacc
     8461 tgcggccagt aatgcaaaag tgaatatttc tgcggggggc ggtggttcag tgatgattaa
     8521 tggtgttgcg acattatcca gtgcttcatc atcgacacgc gggagtgccg cagtacaatt
     8581 tctactgtgt ttattaggtg gcaagtcatg gggtgcatgt gtaaatagct acagaaatgc
     8641 attggcacaa aatgcaggtg tctattcctt caatctgaca ttgtcataca acccgataac
     8701 cacaacctgc aaacaggacg atttattaat tactttagac agtattcccg tttcacaatt
     8761 accagccaca ggtaacaaag caacaataaa tagtaaaaaa agggatatta ttctgcgttg
     8821 taaaaattta ttaggtcaac aaaatcaaac atcacggaaa atgcaggtgt atttatcaag
     8881 ttctgacttg ttaaccaaca gcaacacaat actgaaaggt gcggaagata atgcgtagga
     8941 tttattcttg aaagtaatgg ttcgccagtc acacttttaa atatcactaa cagcagtaaa
     9001 ggatacacaa atttaaaaga aattgcggca aagtcaaaac ttacagatac aacggtttca
     9061 attccgataa cagccagtta ctacgtctac gatacaaaca aagttaaatc tggcgcactg
     9121 gaggcaaccg cattaatcaa cgtgaaatac gactaataac aagcaaaacg agcaagtggc
     9181 taataataaa aaataacaag gtgtctgcat ttccccctca tgatgagggg gcttttttta
     9241 gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt ttaccgtcgg tgtcgctgat
     9301 caacacgtac gcgccaccca tataggtcca gtgcgtcccg gcatcaggcg cgggcaggtt
     9361 acgtaactgc cactgcttaa tgttgtactc atcggtgcgg tacatctctg ggacggtatc
     9421 acctggtttg aaatgcttaa agtcagcaat gaaactactt aactcatact tagaaattcc
     9481 cgaggtattg gtcgaacccg cggtggctgg tgcggcccag gcggcactgg taaccagcaa
     9541 aagcacaccc agcaacatct tatttttaat ggtcataaac tctccacttt ggggatctgt
     9601 aatatcagtc gcatatcttt cctgtcatac aatataactg acaagcgata ttttgttagt
     9661 gatcccatga tttcgtaaaa agtgtaaatg aaaggatgcc ctaaaagcat ccttagcgag
     9721 cagagagtca gcggaaatag ctgatttaag aatcatcctg ggctgaagat aacatgcaag
     9781 cctaaaggat tgagttatcg cattatgccc atgaagccat gtacgcccat atacacaccg
     9841 actaagccaa tcaacagact ggaaaaatag ggggcgcgtt tagcgagagt gttaaatccg
     9901 ctccagcgtt ttgcgacctg ctgaacgctg attgctgcgc caacgcctac ggtgacaagc
     9961 gttaacgcca ggccaatgct gaaactgacg accagtgttg cgcccagtgt cagggctttc
    10021 aactgaatgc atatcaacag cacggtaatt gctgccgggc aggggataaa gccaccggtt
    10081 aagccaaata acaaaatttg ccagttgatg acctctctac catcaaagcg tcgtttaatg
    10141 tcattggcat gggctcgtgc atgggcatcc tgatactcgc catgttcgtg atggtgatga
    10201 tgtccttggt cgtggtgatg ttcgtggtct tgatgatgtt cgtgatcgtg atgatgctca
    10261 taatcatgcc cgtgcatatt ctccagccag ttgcgttcgc cgcgctaggt acgccagaac
    10321 atccagaacg cggtgccaat aatgatcact gcggaaatca gctggagcca cggttctgct
    10381 gattgagcag taaagcgctt actgatcacc atcccgccaa aggcaatcaa ccagaccacc
    10441 gcggtatgcg aaatggttgc tgccagtccg agcatcaccg cctgtttaat ggtgcctttg
    10501 atggcgatga taaacgccgc catcatcgtt tttgagtgcc ctggttccag gccatgaagc
    10561 gcaccaagta agatggcgct ggggatgaag aaccaggcgt ttccttgctg aagaagagtt
    10621 gtaaattcgg tcatgataat aattcttagt attaattcgg caatctgatt ctactccccc
    10681 ccagtacctg atactacccc cccagtagat taatagtgct atgatttttc atattcttgt
    10741 taaccaggtg ttgccatgtc tcatacaatc cgtgataaac agaaactgaa agcgcgtgcc
    10801 agtaagattc agggccaggt cgtggcgctc aagaaaatgc tcgacgagcc gcacgaatgc
    10861 gctgcagttt tacaacagat tgctgctatc cgtggagcgg taaacggtct gatgcgggaa
    10921 gtgattaaag gtcatctgac ggaacacatc gttcactagg gggatgagct aaaacgtgaa
    10981 gaagatctgg atgtcgttct gaaggtgctg gattcatata tcaaataatt tattaacgcg
    11041 attgtaaaac tgccgttttt cctcgtttac aacgggtgcg ctggacatta ccatcctcct
    11101 ctgcgattta tcatcgcaac caaacgactc ggggtgccct tctgcgtgaa ggctgagaaa
    11161 tacccgtatc acctgatctg gataatgcca gcgtagggaa gtcacggacc accaggtcat
    11221 tgcttcttca cgttatggca ggagcaaact atgcaagtcg acctgctgag ttcagcgcaa
    11281 tctgcgcacg cgttacacct ttttcaccaa cattcccctc ttgtgcactg catgaccaat
    11341 gatgtggtgc aaacctttac cgccaatacc ttgctggcgc tcggtgcatc gccagcgatg
    11401 gttatcgaaa ccgaagaggc cagtcagttt gcggctatcg ccagtgcctt gttgattaac
    11461 gttggcacac tgacgcagcc acgcgcacag gcgatgcgtg ctgccgttga gcaagcaaaa
    11521 agctctcaaa cacgctggac gcttgatcca gtagcggttg gtgcgctcga ttatcgccgc
    11581 catttttgtc atgaactttt atcttttaaa ccggcagcga tacgtggtaa tgcttcggaa
    11641 atcatggcat tagctggcat tgctaatggc ggacggggag tggataccac tgacgccgca
    11701 gctaacgcga tacccgctgc acaaacactg gcacgggaaa ctggcgcaat cgtcgtggtc
    11761 actggcgagg tggattatgt taccgatgga catcgtatca ttggtattca cggtggcgat
    11821 ccgttaatga ccaaagtggt aggaactggc tgtgcattat cggcggttgt cgctgcctgc
    11881 tgtgcgttac caggcgatac gctggaaaat gtcgcatctg cctgtcactg gatgaaacaa
    11941 gccggagaac gcgcagtcgc cagaagcgag gggccaggca gttttgttcc acatttcctt
    12001 gatgcgctct ggcaattgac gcaggaggtg caggcatgaa acgaattaac gctctgacga
    12061 ttgccggtac tgatccgagt ggtggtgcgg ggattcaggc cgatcttaaa accttctcgg
    12121 cacttggcgc ttatggttgc tcagttatta ctgcactggt ggcgcaaaat acccgtggcg
    12181 tacagtcggt gtatcgcatt gagcctgatt ttgtcgccgc ccagctcgat tcggtgttca
    12241 gcgatgtgcg aatcgatacc actaaaatcg gtatgttggc ggaaaccgat attgttgaag
    12301 cggtggcaga acggttgcaa cgttatcaga tccaaaacgt ggtactcgac accgttatgc
    12361 tggcaaaaag cggcgacccg ctgctttcac cttcggcggt tgctacgctg cgcagtcgat
    12421 tattgccaca ggtttcatta ataacgccaa acttgcccga agctgccgcc ttgctcgacg
    12481 cgccacacgc gcgcaccgaa caggaaatgc tggaacaagg gcgatcgctg ttggcgatgg
    12541 gctgtggcgc agtgctaatg aaaggtggtc atctggatga tgagcaaagc ccggactggc
    12601 tgtttacccg cgagggtgaa caacggttta ccgcaccgcg cattatgacc aaaaacaccc
    12661 acggcactgg ttgtacactc tctgcggcgt tggctgcact acgcccgcgc catacaaact
    12721 gggctgacac cgtacaggag gcaaaaatct ggctttcatc ggcgttagcc caggccgaca
    12781 cgctggaagt tggtcacggt attggtccgg ttcaccactt ccacgcctgg tggtgagcac
    12841 cgtactatag ctgctttggt tattgtcagc ctttggcact gacattcagg gatgaaacca
    12901 atgcagctaa gaattaccag tcgaaaaaaa ctcaccgctc tattatgcgc tcttgggcta
    12961 atttccatcg ttgcaattta tccgcgtcag acggtgaatt ttttctattc gacagcagtt
    13021 cagattacag actacatcca cttctacggt tatcgcccgg ttaaatcttt cgctattcgc
    13081 attcctgcca gttacaccat tcacggatta gatgtttcgc gctggcagga gcgtatcgac
    13141 tggcagcggg tggcaaaaat gcgcgacaac gatatccgct tacagtttgc ttttattaag
    13201 gctacggaag gcgaaaagct ggtggacccc tatttttcgc gtaactggca attaagccgc
    13261 gaaaatggcc tgctgcgcgg ggcgtatcat tatttctccc cgtcggtatc cgcttcagtt
    13321 caggcgcgat tatttctgca aacggtggat ttctcacaag gcgatttccc tgccgtgctg
    13381 gacgtagaag aacggggaaa attatcggca aaagaattac gcaagcgggt aagtcagtgg
    13441 ctaaaaatgg tcgaaaaacg cacgggaaaa aagccgatta tttactcagg agccgttttt
    13501 tatcacacca atctggcggg ctatttcaat gaatatccgt ggtgggtggc tcactattat
    13561 caacgtcgtc cggacaatga cggcatggcc tggcgcttct ggcagcattc cgaccgtgga
    13621 caggtagatg gcatcaatgg tccggtggat tttaatgtgt ttaatggcac ggggatgagt
    13681 tgcaggcatt cgttgatggg attaaagaaa cgccttaaat aagaagcagt gatatactcc
    13741 caccagactc cctggcagga aaaagacaaa tggaacaagc gcatacccag ctgattgccc
    13801 aactgaacga gcgtatttta gcggcggata acacgccgct ttatattaag tttgccgaaa
    13861 cggtaaaaaa tgccgtgcgc agcggggtgc tggagcatgg caatattttg cccggtgagc
    13921 gtgatttaag tcagttaacc ggcgtttcgc gcattacggt gcgcaaggcg atgcaggcgc
    13981 tggaagaaga gggcgtggtg acgcgttcgc gcggttacgg cacgcagatc aacaacatct
    14041 tcgaatactc gttgaaagaa gcgagaggtt tttctcagca ggtggtattg cgcgggaaaa
    14101 agcccgatac gctatgggtt aataagcgtg tcgtgaaatg tcccgaagaa gttgcgcagc
    14161 agctggcggt cgaagcggga agtgatgtct ttttgcttaa gcgtattcgc tatgtcgatg
    14221 aagaagcggt atcgattgag gaatcgtggg ttccggcgca tttaattcat gatgttgatg
    14281 ccatcggaat ttctctttat gactatttcc gcagccagca catttaccca caacgtacgc
    14341 gttcccgcgt tagcgcccgg atgccggatg ccgagtttca gtcacatatt cagttagata
    14401 gcaaaatacc ggtgctggtg atcaagcaag tggcgcttga ccaacagcaa cggcctattg
    14461 agtacagcat tagccactgt cgcagcgatc tatacgtttt tgtgtgcgag gagtagttcc
    14521 tcgcgcgttg gcgcacaatc accgccccga tgcccgacaa cccacgacgc cactgcattg
    14581 cccagtaata cggcatccgc cagtggcaga ccagaggcca gcccggcaag tacgccacct
    14641 gcatgactgt cgcccgcccc aatggtgtct acaacttgcg ttggaaatgc cggaatgcag
    14701 ccagaagcgt cgttgctgaa ataccatgcg ccttctttat cgagacgaac gatcaacggc
    14761 gcggcaaatt tctcctgcca ttgcttgcca agtgttgtta tctctgcgga taaagcaaaa
    14821 cgttcggcgg caatctcagc ctcttgacga ttgagtgaca ctaaaggtcg acaggccatg
    14881 atccgcgcca gtaatgcgtc cgggatatcg ccaatacgtg ggcaaaaatc gataaacggc
    14941 gtcacgtctt gcagcttttc cagccattcc actaacaatt cgccgcaggg cgaggccagt
    15001 tgataaccgg aaaaatagag caggttgcca ggcgcaacgg ttaatcgtgc caaccattgg
    15061 cgattccatt gattttccac accgctgaat gacataaaag tgcgttcgcc atccggctca
    15121 accagtgcca gacaccagcc gttatcgctt tcggcgttat cgatcaggct gattaacccc
    15181 tctttcgcca tccggttgcg aataatctcc gcccacacgc cctgaccgag cggcaaggca
    15241 ttacccgctt cgatgccgag gcgctttaac gccacggcaa tattcagtgc gcagccgcca
    15301 acgttaacgc tctgctgttt cagttcgata tcgcacccac gccaggggag ggcataagcg
    15361 tcggcgatca cgtcaatgac cgccgcgccg acgaccatca ccggctgacg cgtggttaat
    15421 tcaggcagca gtgtgtgtaa tcgagcgccg ctcataccgc ctcccgttgt tgacgatatt
    15481 tcgccagcgc tgtggcatag cggttgaaat caagctgatt taccgcatcc agctccgcct
    15541 ttaacgcagg atcgacagcg ttaatgccat gcaacgcgcc gcaaatcgcc gtcgccatag
    15601 cacctatggt gtctgtgtcg ccgccaagat tggcgcacag gacggcgcag cggtttgggt
    15661 cggtttgtgc cagttcaact agcgcaatgg cgcacggaac ggactcaata gtgctggtgc
    15721 ctgcgccaac gacctggtaa agctgttcgc tggcggattc ggtgccgtcg gcattacgca
    15781 caattttcag cgcaatctcc agacgtgccg ccagtgaggc gctgaacgtg gtggtgcgtt
    15841 tttgctgtgc atgtcgcgca attgaaggca atgaatcaac aatcgctgac cagcgttctc
    15901 cgtcaatggc acgagaaatc gcccatgcga tgactaccgc gcctgcaacc gccagatcgg
    15961 atttatgggt cgggctggac gccagcgcca catcatcaat aaaggaatca acatcacgcg
    16021 ctggaagcaa acaacctaat ggcgagacgc gcatcgctgc gccgttggtt acgccgttgt
    16081 tttccagttc agcaacgggt ttaccgtcgc gaatggcgtt aagcgcaatc ttcgaggtcg
    16141 gacctagtac gtttttgtta aaggcgtcga aacgcaacgc ccagtcgaga atattacgcc
    16201 caatcagatt cggatcgatc ttgccttcac attccagtaa cgcatccgcc agacacaggc
    16261 catcgaggta tcgtcggtga attcggcgcg gttaaaataa caggccgcgt tattctcctt
    16321 tggtccaggc agaaaacggt caatccagcc aaagtgtgct ttaacgcggc tgcgtgtcca
    16381 aagctcggag ggcatcccca tcgcatcccc taacgcctgc ccataaagag caccgagaat
    16441 acgttctgtt ttcatttaac ttccccttgt gtcaacgcaa tatctcgatc atcgaccttg
    16501 atagccgtaa tttcgttgtc ggattcgcgg aaaaaaatca tgaacagcac ggcgataatg
    16561 gcaatcatca ccgcgccgaa agtccacatc ccggaccagt tgaaagtcag tccgtttacc
    16621 ggttcctgat aagcgaacat cttttccatc atcacaccgc caagacgata gccgagcaaa
    16681 ctgccgaagc cctggcagca gagcgtgatc agcccctgcg cagcggtacg catatgcacg
    16741 ggggcttttt tatcgacata gatgtaagcg gtaacgtagt aaaaatcgta acttacgccg
    16801 tgaagcaaaa taccgaggaa cagtaacgcg taggtgaaat attcatccgc actaccgtaa
    16861 ataaagaagc catagcagat cgcagcggtg accagaccaa gcaataatac ctttttgata
    16921 ccaaagcgtt tagtgaaaaa cggcaatgcc agcataaaga agatttcaga gaactggccg
    16981 agcgtcatcc agccggtggc gtttttcatg ccaacttcgg tcagataacc gttggcaaag
    17041 atgtaataga acgccagtgg catcgcaaac aggaatgaac agaagaaaaa gacgaggaag
    17101 tttttatcgc gcagcaggat cagcgcatcc aggccgagca tgactttaat gtccattttg
    17161 ccggtgcttt ttggcggcgt gtcgggcagg aaaaacgcaa acacaccgag cagagcagaa
    17221 cttccggcgg taatcagcag cgggatgtta gtcggtgaga tatcggcata ccccagcatt
    17281 tgcggcaaga aaccacatgc cagaccggag gcaatccagc cgatagtgcc catcacacga
    17341 atgcgtggga aatcacgctc cacatccggc acgttggcaa aagcgatgct gttagtcagc
    17401 gcaatggtcg gcatataggt tagcgagtag gccagcagta acggaaagaa cccggcaaaa
    17461 gtggtctgtt gcgcagcgaa atacatcagc acagcaccag cgaacatcaa taccgccagc
    17521 actttctgcg ccgagaaaaa gcggtcagtg atggagccaa ccagaattgg cgacaggatc
    17581 gccgcaatgg ctgtgcaggc atacgaccag ccaatttctc cggcactaaa accgctttta
    17641 cttaaccaca accacaatgg cacaaaccac gcgccccaga taaaccattc aacaaacatc
    17701 atgagcgaca gctttgctgt tgttttcatc ttttaattcc ttcatgacag gtaatgtaag
    17761 gtacgcgatg acaataccac ataataatgc cttttgaata cctttgcggt gaaaacttga
    17821 ccgcgctaac attttttctg atgaatcgag ccaacagaaa acgctgaaaa aacatccaaa
    17881 agatggaaaa actcgcccgt aacgactagg attatgattg cccgcaaaag gcgggccagg
    17941 acaatccata gccgatatcc aatcggaatt tacgggagca tagtaatgac agatattgcg
    18001 cagttgcttg gcaaagacgc cgacaacctt ttacagcacc gttgtatgac tattccttct
    18061 gaccagcttt atctccccgg acatgactac gtagaccgcg tgatgattga caataatcgc
    18121 ccgccagcgg tgttacgtaa tatgcagacg ttgtacaaca ccgggcgtct ggctggcaca
    18181 ggatatcttt ctattctgcc ggttgaccag ggcgttgagc actctgccgg agcttcattt
    18241 gctgctaacc cgctctactt tgacccgaaa aacattgttg aactggcgat cgaagcgggc
    18301 tgtaactgtg tggcatcaac ttacggcgtg ttggtgtcgg tatcgcggcg ctatgcgcat
    18361 cgcattccat ttctcgtcaa acttaatcac aacgagacgc taagttaccc gaatacctac
    18421 gatcaaacgc tgtatgccag cgtggagcag gccttcaaca tgggcgcggt ggcggttggt
    18481 gcgactatct attttggctc ggaagagtca cgtcgccaga ttgaagaaat ttctgcggcc
    18541 tttgaacgcg cgcacgagct gggcatggtg acagtgctgt gggcctattt gcgaaactcc
    18601 gcctttaaga aagatggcgt tgattaccat gtttccgccg acctgactgg tcaggcgaac
    18661 catctggcgg caaccatagg tgcagatatc gtcaaacaaa aaatggcgga aaataacggc
    18721 ggctataaag cgattaatta cggttatacc gacgatcgcg tgtacagcaa gttaaccagc
    18781 gaaaacccga ttgatctggt gcgttatcag ttagctaact gctatatggg ccgggctggg
    18841 ttgataaact ccggcggtgc tgcaggcggt gaaactgacc tcagcgatgc agtgcgtact
    18901 gcggttatca acaaacgcgc aggcggaatg gggctgattc ttggacgtaa ggcgttcaag
    18961 aaatcgatgg ctgacggcgt gaaactgatt aatgccgtgc aggacgttta tctcgatagc
    19021 aaaattacta ttgcctgata cgcctcatac gaacagcacc accccgtagg acggataaga
    19081 cgtttacact gcatccggca acctatgcct gatgcgacgc ggaagcgtct tatcatgcct
    19141 acatagcact gccacgtatg tttacaccgc atccggcata aaaacacgcg cactttgcta
    19201 cggcttccct atcgggaggc cgtttttttg cctttcactc ctcgaacaat tttcatattg
    19261 ttgtttttgt gatcattatc tcgacattta aaagcaaata atttcattat attttgaaat
    19321 cgaaagcaaa cgacaagata tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa
    19381 cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg aaacgatgca
    19441 agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg
    19501 cacatttact catgctggta cagaaaatct gttggcgctg gtcagcgcga tggcgaagca
    19561 atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg atatcgctca
    19621 gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc cttttgcgca
    19681 aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg tcagcgtcga
    19741 ggcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg aagtcgatgc
    19801 gttgtacacc aaccccgcac aggcgcgtga atttgccgag gcaaccggga ttgattccct
    19861 ggcggtcgcc atcggcacgg ctcacggtat gtatgccagc gcaccggtgc ttgatttttc
    19921 tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg
    19981 attatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa tcaacgttgc
    20041 aacggagctg aaaaatgcct tcttgcagtc gttaaaaaat tacctgaccg agcaccccga
    20101 agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag
    20161 caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc atttaacaag
    20221 gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc ggcatatgtt
    20281 cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat cgcaacagca
    20341 cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc ggttataccg
    20401 gaatgacacc ggcagacttt cgcgaatttg tttttacgat tgccgataaa gttgggtttg
    20461 cacgcgaacg cattattctc ggcggcgatc atctgggacc aaactgctgg cagcaagaaa
    20521 atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt cgtgctggct
    20581 tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggataccata ccgttagcac
    20641 cagaaacggt tgcggaacga gctgctgtgc tttgctttgc tgcggaaagt gtggcgacag
    20701 attgccagcg tgagcaactg agctatgtca ttggcactga agttccggtt ccgggcggtg
    20761 aggccagcgc cattcagtca gtacacatca cccatgttga agatgccgcc aatactttac
    20821 gtacgcatca aaaggccttt attgcccgtg ggctgacaga ggcgttaaca cgtgtgattg
    20881 ccatcgtggt gcagccgggt gtggaatttg atcacagcaa tattatccat tatcagccgc
    20941 aggaagcgca ggcgctggcg caatggatag aaaacacccg aatggtttat gaagcacatt
    21001 ctaccgatta ccagacccgg acggcttatt gggaattagt ccgcgatcac tttgcaatat
    21061 tgaaagtcgg tcccgcatta acctttgctt tacgcgaggc gatatttgca ctggcacaaa
    21121 ttgagcagga acttatcgcc cccgaaaatc gcagcggttg cctggcggta attgaagaag
    21181 tgatgctcga cgaaccgcaa tactggaaaa aatattatcg cacgggtttt aacgattcat
    21241 tactggatat tcgttacagc ctgtcggatc gtattcgtta ttactggccg catagtcgga
    21301 ttaaaaatag cgttgaaacg atgatggtga atctgcaagg cgtggacatc ccactgggca
    21361 tgattagtca gtatcttccc aaacaatttg aacgcattca gtccggggaa ttatcagcaa
    21421 tgccgcatca gcttattatg aataaaattt atgatgtttt gcgcgcctat cgctacggct
    21481 gtgcggaata aggacggtat atgactaacc tgtttgttcg tagcggaatt tcttttgtcg
    21541 atcgtagcga agttttaacc tatatcggta atgagatgct cgcgaaaggt gtggttcatg
    21601 atacctggcc acaggcatta attgccagag aagcagaatt ccctaccggg ataatgcttg
    21661 agcagcacgc cattgcaata ccgcattgtg aggcgattca tgctaagtcg tcagccattt
    21721 atctgttaag gccaacaaat aaagttcatt ttcagcaagc ggatgatgat aacgacgtgg
    21781 cggtatcgtt ggttattgcg ttgattgtgg aaaatccgca gcagcaattg aaacttttac
    21841 gctgtttatt tggcaagtta caacagcccg atatcgtcga gacactaatt actcttcctg
    21901 aaactcagtt aaaggaatac ttcacaaagt atgttttaga ttcagacgaa taaatccctc
    21961 tgtaacaata aaaaggacta tttatgaaac gcaagattat tgtcgcttgc ggaggcgcgg
    22021 ttgcgacctc tacgatggcg gcggaagaaa ttaaagagtt gtgtcagagt cataatattc
    22081 ctgttgaatt aatccagtgt cgggttaatg aaatagaaac ctatatggat ggtgtgcatt
    22141 tgatatgtac cactgccagg gtggatcgta gttttggcga tattccgtta gttcacggca
    22201 tgccttttgt ttctggtgtc ggtatcgaag cattacaaaa taaaattctg actatcttac
    22261 aggggtgacc tatgttttca gaagtcatgc gttatattct cgacctcggc cctacagtga
    22321 tgctgccggt tgtcatcatt attttttcta aaatattagg catgaaggca ggcgattgct
    22381 ttaaagcggg tctgcatatc gggattggct ttgttggcat tggccttgtg attggcttaa
    22441 tgctggattc cattggtccg gcggcgaaag cgatggcgga aaatttcgac ctgaatctgc
    22501 atgtggtcga tgtcggctgg ccgggctctt caccaatgac ctgggcgtcg caaattgcgc
    22561 tggtggcgat tccgattgcg atattggtta acgtggcgat gttactgacc cgtatgacgc
    22621 gggtggtaaa tgttgatatc tggaatatct ggcatatgac cttcaccggc gcgttgctgc
    22681 acctggcaac cggttcatgg atgataggga tggcgggtgt ggtaattcac gcggcgtttg
    22741 tttataagct ctgcgactgg tttgcccgcg atacccgaaa tttctttgag ctggaaggta
    22801 ttgctattcc gcacggtacg tcggcgtata tggggccgat tgcggtgctg gtcgatgcta
    22861 tcatcgagaa aatcccaggc gttaaccgaa ttaaatttag cgccgacgat attcagcgca
    22921 aatttggccc ttttggcgaa cctgtcaccg tgggttttgt gatggggctg attatcggca
    22981 tcctcgcggg ttacgatgtc aaaggtgtat tgcagctggc ggtaaaaacg gcggcagtta
    23041 tgctgctgat gccacggatg attaaaccca tcatggatgg tttaacgccc atcgctaagc
    23101 aggcccgtag tcgtttacag gcgaagttcg gcggtcagga gttcctgatt ggcctggatc
    23161 ccgcattact gctggggcat acggcggtgg tatcggcaag cctgattttt attccgctca
    23221 ccattttaat tgctgtttgt gttccgggta atcaggtact gccgtttggc gatcttgcca
    23281 ctatcggctt cttcgtggcg atggcggtcg cggtgcatcg tggaaatctg ttccgcacct
    23341 taatctcggg tgtcatcatt atgagcatca ccctgtggat cgcgacgcaa actattggtt
    23401 tgcacaccca accggcggct aatgctgggg cgttaaaagc cgggggtatg gtggcttcaa
    23461 tggatcaggg cggttctccc attacctggt tactgattca ggttttctcc ccgcaaaata
    23521 ttcccggttt cattattatc ggcgcaattt atctgaccgg tattttcatg acctggcgta
    23581 gagcgcgtgg ttttactaaa caagagaaag ccgttctcgc agaataattt ttacctgagg
    23641 gggaggttat cccccttaac aatcaggagt ttttatgaaa tcagtagtga atgatactga
    23701 tggtatcgtg cgcgttgcaa aaagcgtcat tcctgaaatt aaacatcagg atgaggtgcg
    23761 ggtaaaaatt gccagctcgg gcttatgtgg ttccgattta cccaggatat ttaaaaatgg
    23821 tgcacattat tatccaataa cgttaggcca tgaatttagc ggctatattg atgcggtggg
    23881 atccggtgtt gatgatttac accctggcga tgcggttgcc tgtgtgccgt tattaccctg
    23941 ttttacttgt ccagagtgtt tgaaagggtt ttattcccag tgcgcaaaat atgattttat
    24001 tggctcgcgg cgtgatggtg gatttgctga atatattgtc gttaagcgaa aaaatgtctt
    24061 tgctctaccc acggatatgc ctattgagga tggggctttt attgagccga ttaccgttgg
    24121 cctgcatgct tttcatttag cgcaaggttg tgagaataaa aacgttatta ttattggtgc
    24181 cggaaccatt ggcctgctgg ctattcagtg cgctgtcgcg ctgggagcaa agagtgtgac
    24241 ggcgatcgac attagctcag aaaaactggc actggcaaaa tctttcggtg cgatgcaaac
    24301 atttaacagt agcgaaatga gcgcgccgca aatgcagagc gttttacgcg aactgcgctt
    24361 taatcagctt atcctcgaga cggctggcgt accgcaaact gtcgaactgg cggtagagat
    24421 tgccggtcct catgcccaac tggcgctggt gggcacgttg catcaggatc tgcatttaac
    24481 atcggcaacg tttggcaaaa tattgcgtaa agagctgacg gttatcggca gttggatgaa
    24541 ctattccagc ccttggccgg ggcaggagtg ggaaacggcg agccggttgc tgacagaacg
    24601 taagttaagc ctggagccat taatcgctca ccgtggaagc tttgaaagct tcacccaggc
    24661 ggtgcgtgac atcgctcgta atgccatgcc gggcaaagtg ttgctcattc catgaaaccg
    24721 cgggccagcg tgatgctggc ccggtattgt gcaaaacaga tcattcacca atggtccccc
    24781 ttcgtttaca ctagccacaa ttgaatatgg gtaaatgacg atgaattcat tcgagcgaag
    24841 gaataagatc atccaattag tgaatgaaca gggaaccgtg cttgttcagg atctggcggg
    24901 agtatttgct gcctcggaag cgacaatccg tgccgatttg cgctttctcg aacaaaaagg
    24961 cgtggttacg cgctttcatg gcggtgcggc gaaaataatg tccggtaata gtgaaaccga
    25021 gacccaggaa gtcgggttta aagagcgatt tcagctcgcc agcgcgccaa aaaacagaat
    25081 agcgcaggcg gcagtcaaaa tgatccatga agggatgacc gttattctcg acagcggaag
    25141 taccacaatg cttatcgccg aaggattaat gaccgctaaa aatatcacgg tgattaccaa
    25201 cagtctcccg gcagcgtttg ccctttccga aaataaagac attactctgg tcgtctgtgg
    25261 tggcacggtc cgccataaaa cgcgctcgat gcatggttcc attgccgagc gttcattgca
    25321 ggatattaat gccgatttaa tgtttgtcgg tgctgatggt attgatgcgg ttaatggtat
    25381 tacgaccttt aatgaaggtt attcgattag cggggcgatg gtcaccgccg caaataaagt
    25441 gattgccgtt ctcgattcat cgaaatttaa tcgtcgcggt tttaatcagg tactgccaat
    25501 cgaaaaaatt gacattattg ttaccgatga tgctgtgtca gaggtggata aattggcatt
    25561 gcagaagaca cgggtaaaat taattacggt gtagtattgt tctgtatgcc ggataaggtg
    25621 tttacaccgc atccggcatt acggattctc cctggttcaa gagagaatag tttgattaac
    25681 gcaataatgg acaatctggt ggtaatcgac aacgcaacgc cgccggaagt atttcaatat
    25741 ggaagttttg cccactcaac ggttcgccat caagattaaa agtgatttcg tgtggggctt
    25801 gtatatcaaa ccacgacgaa gcgccttcga taatattcgg gttatcttcg tcagatttta
    25861 aggttgatac gagagccgga agaatttcat cgccggtaaa aatgcgcagt tgcagcaagc
    25921 catcgttaat taacgcgttc gggcacaatt gttgaccgcc accggcctga cgcccgttac
    25981 caataccaat gaccagggcg tcaccttgcc agtgaaagtt ttcaccgcgg atttcacaac
    26041 ggtccggttg cagagtatcc atgcgcatta agccatgaat gatgtaagag acgccaccca
    26101 gcgcggcttt taatttttcc ggtgtttctg tggtaatacg cgtcccaaat ccgcctgtcg
    26161 ccatattaat aaaacaggtt tgtttgttga cctgcgccat atctatcgca atggcattac
    26221 cggcaattgc cagtttcagc gccttatcca gtgcctcagg aatccctaca ctggtggcaa
    26281 aatcattggc agttcctaat ggcaaaattc ccagcgcggg tatgtcatcc ccctcacact
    26341 gaatcaacgc cgtagaaact tcattaatgg tgccatcgcc accaccggca atcaccgttg
    26401 cgacgcccaa cttccgggcc tcctctacaa atcgtgcggc atcacctttc tcccaggtga
    26461 cccgcacatg gatcgtcatt ccttcctcac gcaacagcat aattgcttcg cgcaagggta
    26521 gattgtcagt acttttacca ttaagaatca gtaagctggc gggaaattct gccatgatcg
    26581 tatgtgcctt tatgattggt ctgtagatag tgtagagcag aaaacaaaag gtggagtcag
    26641 aacaggatga aagtcggagg ataaaaagtc agttcaagta tggaggattc agtggttacc
    26701 accaggagct tgcaatgggg acagcgaata ctaacggctg ccaccgataa atttcaaaaa
    26761 agagcatata cctaatattc aactaaacag tggcatcttc aatataatat attaaagccc
    26821 ccatggagtt accctgaagg gcctcaatgt ccgtaattcc tacttatgta ggaaatgttg
    26881 tacagaacat ttattataat cctattcaat tataataatc atgccattat tatatttaaa
    26941 cactagagag tgtcgttggt atttaatggg ggaaggtgag atgaaaaaga tagctgctat
    27001 atcattaatt agtatttttc ttatatctgg ttgtgctgtg cataatgatg aaacaagtat
    27061 cggtaaattt ggtcttgcat ataaaagtaa tattcagcgt aaactcgata accaatacta
    27121 caccgaagcc gaagcttctt tagccagggg cagaatatct ggtgcagaaa atatagtaaa
    27181 aaatgatgca gcccatttct gtgttactca gggcaaaaaa atgcagatag ttgacctgaa
    27241 gacagaaggt gcaggattac atggcgtcgc tcgtctgaca ttcaaatgtg gagagtgaga
    27301 atattttttt gtaagcgtca aacatgcgcg ttctggctgt gcgtagccgg aacctgggcg
    27361 agcacgatac cgttacgtga aaggcattgt gctatgaagg gggattctat cgatgtggtc
    27421 aatggaagac ggttatcagg gatcaatcct tctttacagc ttatgtacaa aaaataagcc
    27481 cgtgtaaggg agattacaca ggctaaggag gtggttcctg gtacagctag cattttatgg
    27541 attatgtttt tcagcgaaac ggatgataac cttaataaat gcagctgtat gtgatcggtt
    27601 tctaagaatt ttccatccgg gaaaaataat cgaaattaat cacttaccgt gggggttacg
    27661 cgtggtttcc ccggagaaat tacgcattag cagcgcgtaa tttagctcaa gatcctgcgg
    27721 gaccgggagc cacacagtat aaccatcgcc tggtgcgacc ggcatagctt cgcctttggc
    27781 gttttccatg tgctcaaggg taaagttaat gttgccttgc ggcgtcatca gctcaagact
    27841 gtcgccaacg gagaatttat ttttcaccgc taccgctgcg aggtccccct tgcgctcacc
    27901 ggtaaactca ccaacaaact gctggcggtc agaaactgaa taaccgtatt cgtagttctg
    27961 ataatcgtcg tgagtatgac gacgcaggaa accttcggta tagccacgat gcgccagacc
    28021 ttccagagtt tccagcaggc tggtatcgaa cggttttccc gcagcggcgt catcgatagc
    28081 tttgcggtaa acctgtgcgg tgcgtgcgca atagtagaaa gatttggtac gaccttcgat
    28141 tttcagcgaa tgcacgccca ttttggtcag gcgttctaca tgggcgatgg cgcgcagatc
    28201 tttcgagttc atgatgtaag tgccgtgctc atcttcaaac gcggtcatat actcgcccgg
    28261 acgctgggct tcttcgatca taaacacttt gtcggttggc gcgccgatac ccagcgtcgg
    28321 ctcaacattt tgcaccggaa tcggctcgta cttgtgtacg atgttgccaa cgtcatcttc
    28381 tttcccttcc tggacgttgt actcccagcg gcaggcgttg gtgcaggtgc cctggttcgg
    28441 gtcgcgcttg ttgatatagc cagagagcag gcagcgaccg gagtaggcca tgcacagtgc
    28501 gccgtgaacg aagatctcga tctccatatc cggcacctga ttatggatct cttcaatctc
    28561 ttccagcgac agctcgcgag agaggatcac gcgggtcagt cccatttgct gccagaattt
    28621 caccgtcgcc cagttcacgg cgttagcctg caccgagagg tggatcggca tttcagggaa
    28681 gtgctcacgc accagcataa tcagccctgg atcggacata atcagcgcat ccggccccat
    28741 ttccaccacc ggtttcaggt cacggataaa ggttttcagc ttggcgttgt gcggtgcaat
    28801 gttgaccacg acataaaact ttttccccag cgcgtgggct tcattgatgc cgagctgaag
    28861 attttcgtgg ttgaattcgt tgttgcgcac acgtagggag taacgcggct ggcccgcata
    28921 aacagcatct gcgccataag cgaaagcgta acgcatattt ttcagcgttc ccgccgggga
    28981 aaggagttcc ggtttaaaca taattctctc gttctgatga caggtcagat ccgcttcacc
    29041 tgatgaagcg gttaggggag gcccccactt taagggcggg cattgtagcg ctgtaggggg
    29101 gatctggtaa agcgccggac gccaacaacc cacgcccggc atcgtaatta cgtattgtct
    29161 ttcaccgtct ggcttgtgcc attggcttta acggaggcaa ttccggtttc acgagattgc
    29221 gcggtggcgt acatctggct ggagccgata atttgatgat tagcggcttt cagattgaaa
    29281 tagaatttgc cgttgcttgc cgttttttct catagcgctc ttcttgcggg ctgttgctac
    29341 gcaccgacgc gatgcccttt tccgcagagg cttttgaggt atacagctcg ctggtgagga
    29401 tagtctcacc attgcccgct tttagcacaa accggaactg attatcactg ctcttactga
    29461 gttcaaacca accagccata atgactcctt gattgaaatt gcttatcgaa aacagcatct
    29521 aaaggtgctg tttactgatt aaagtatgag cgattttctg atgcctgcca gggcggagag
    29581 ataatgtcag tttgctgacc atgcgtggaa taatgttgcc ggatcgggtc tgaacgccat
    29641 ctccggctaa caaagtaatg tcgctaaaac taaacgatgc ggcaggcgtc ggcttcccag
    29701 cggtaaccaa cgccgtaaac ggcgcggata aatgactgtt cggcgtcgag agattccagc
    29761 ttgcggcgca gatttttaat gtggctgtca atggtgcggt cggttactac gcggtagtcg
    29821 tcataaagat gattgagcaa ttgctcgcgg gagaacactt ttcctggttc gtgagagagc
    29881 gttttaagca gacgaaattc cgcaggcgtc aggtcaagca ttttaccgcg ccatgaagcc
    29941 tgaaaacgac cttcgtcgat aatcaacggg ctttcggcat cctgttgctg aagttcgcgt
    30001 tgcggtttac aacggcgcaa aatggttttg acacgcgcta ccacttcccg tgggctgtac
    30061 ggcttgcaga tgtagtcatc ggcgccaatc tccagcccca gcaggcggtc gatctcttcg
    30121 atttttgccg tcaccatcac gatcggaaca tcggaaaaac gacgaatttc ccggcataat
    30181 gtcaggccat cggtgccagg gagcatcaga tccaacagga tcagatccgg tggtgtctgg
    30241 cgcacatacg ccagtacctg atcgccgtgg ctgataagcg tcggcgcata gctcgcagca
    30301 cgcagataat caatgagcaa ctgccccagc ttcggttcat cttccacgat caaaatacgc
    30361 ggtgtgtttt cgtcgattgg taactcggtc atacttctct ctgtaaatcc cgttccagcg
    30421 gtaactctac tgtaatgctt accccgccaa aaggcgaatg ggcggcaata atgcggccat
    30481 tatgggcttc aacaatgttc aggcaaatcg ccagccccag cccggaaccg ccgctggcgc
    30541 ggttgcggga accttcggtg cgataaaaac gttcaaacaa tttttgtagc tgatcgtcac
    30601 tgacacctgg cgcactgtcg gcaaaggtca ggcgcaccgt tttgtcgtgc tgctcggcag
    30661 agattttcag gctgccgccg ctgtcagtgt agcgcaggct gttttccagt aagttattga
    30721 ataactgcat taaacggtcg cgatcgccaa atacggtgat actgtctggc agggaaaatt
    30781 gcagtttcag gccacggctg gcgaatcgtt cgcgaaatgc gccgcccgcc acttccagca
    30841 gtgggatcaa atctaccggt gctttttgat aggcgagagc gccttcatca gacatcgaca
    30901 actgatgaag atcgtcaacc agtttggtca gtgtaccgac ctccgcctgt aaagacgcca
    30961 ccgtctccgg cgtgaatttg cgcacgccat cctgaatggc ttccagttca ccgcgcagca
    31021 ccgctaatgg cgtacgcagt tcgtgagaaa tatcggccat aaaatcgcgc cgcatttgct
    31081 ggtttttctc cagcgtgctg gcgagctggt tgaagtcttg cgccagtttg cccagttcat
    31141 cttcactggt gggcgttacg cgggtagtga aatcgcccgc cgccagtttg tgcgtgccat
    31201 cgacaagtcg ttttaccggt gccagcaaac cgcgcgccag cagaaaggtg gcgagcgccg
    31261 cgagtaacgt tgccagggcg acgatcagcc agctggtttg ccgctgttgt ttatcgaaat
    31321 tgatatcagt attgcgggtc agccgctcaa caggggaggc aatcaccgcg cccacttccg
    31381 caccgttgac cagaatgggt cgccgtgtac cgtcaggtgg aatcggcgct cgcggaccaa
    31441 ccagtacttt gttgttttga tcgaccaccc agaactgggt acgccagccg tgcggcggca
    31501 tacccgggcc gggtttatct tccgaattat cgtgttcaaa tgaacgcagg atctgaaaga
    31561 caaagcgatc gttgttgcgc aggaagcgcc agttaccatg ctgcgcatac tgctcgccaa
    31621 gcgcatcact taacagctgt aatcgctgtt cattgccgtg tttgatgtaa tcaataaagc
    31681 cacgctcaaa actgatacgc accgcccagt gcatactgat cagcaagaca atgcaggtgg
    31741 cgaaaatcgc cagaaacagt ttgccggtaa taccgggtcg ccagaacttc attgcgcgct
    31801 cctttttcgc cgcgaaatag ctacattttg atgcgtatcg ttcggcactc tggcaaagat
    31861 gaacgccgga agggcgatga taaacgccat gctaagccag gtgtacataa agacggtttg
    31921 tgtggtgccg ctgtcgacgc tgatatgctg cgaaccaaaa agccccagca acagcccggc
    31981 gatggtgacg ccgatactca tcgacaattg cataatcatc gacagcaggc tgtttccgct
    32041 gctcgccaga ttgtccggga gatctttcag cgtcagggtg ttcatggagg agaaacgggt
    32101 cgagttgacc atcccttgta aaaacaggac gaacggcaaa acgtagtacc agcccaacag
    32161 agcggtagtc ataaacaaca gggtgaccag cgacagaccc agcgtggtcg ctaccagtac
    32221 ccgacgataa ccaaagcgat tcaccacctg taccacaatt cgcttcattc ccatgctgcc
    32281 aagcaccatc gggatcatca tcagtccggc atgaaacggc gagaaaccga ggccaatttg
    32341 caggaaaacc ggtgtcataa agggcaacat gccactgcca atacgtccgg caaagctccc
    32401 cgccaggccc agcgaaaagg tacgagtgcg gaacagtttc agactgaaca gggcgcggtt
    32461 gttatttctg gcgtgcagca gataaagcac cagtgccacc acgccaactg cgaccaggcc
    32521 agcaatcgcc agcggcgata aacctgtacc tttactgccg tccagcgcca gggttaatac
    32581 cgccatgcca accgccagca ataaaaagcc ggaaagatca aagcgccgcg tctgcatggt
    32641 gtagttcggc attaacatca atgtggcgat cgcaccgata atccccaccg gaatgttgat
    32701 caaaaagatc cagtgccacg acgcgtattc taccagcaga ccgccgagcg ccggaccgag
    32761 cagcggaccg acctgaccgg gtaacgtgac aaaggtcatc gccgccatat attgctcgcg
    32821 cggtacgatt ttcatcaccg tcaacctgcc gaccggcacc atcatcgcac cgccaacgcc
    32881 ctgtaacgcg cgtgccagca acagttcgtt cagcgtgccg gaaagcgcgc aaaacagtga
    32941 accgagagta aacagcacga tggcggtaaa gaaaatattg cgcacgccga ctttgtccgc
    33001 cagccagccg ctggcgggca gcatcaccgc cacggtcagc acataagaga caatgaccat
    33061 gtgcatatgc aacggacttt ccccgaggct ttgcgccatt gagggaaggg cggtgtttac
    33121 gatggtggtg tccagcgact gcataaagaa gccgaaagcc acaatccata attgccagcg
    33181 ggtgctgtcg ggaagatctg tcatttactc ggttaccgtt tgtttaggtt tacgcgaaaa
    33241 acgcagccgc agacggtcga aaaagagata caccaccggc gtggtataca gcgtcaggag
    33301 ctggctcatc accagaccgc cgacaatggt gatctccagg ggttgccgca gctccgagcc
    33361 gtcaccgccc gacaatacca gcggcagcgc gccaaacagc gccgccaggg tggtcatcat
    33421 aatcgggcga aaacgcagca gacaggcctg gaaaatagct tcctgcggcg tcaggttacc
    33481 gtgccgttgg gcttcaagcg caaaatcgac catcataatg gcgtttttct tcacgatgcc
    33541 gattaatagc atgatcccta tcagggcgat taggctgaac ggggcattga acagctccag
    33601 cgccaacagc gctccaacgc ccgccgaggg cagggtggag agaatcgtca gcggatgtac
    33661 gtaactctcg taaaggatac ccagcacgat atacaccgtg gcgatggcgg cgataatcag
    33721 gatcacctgc gagttcatcg tctcctggaa aacctgcgcc gtgccggcaa aactgccgcg
    33781 caccgtcgaa ggcacaccaa gctgggtcat tgcgcgatcg atcgccgcac tggcgtccga
    33841 gagcgatttt ccggtcggca ggttgaacga gatggtcgag gccgccgata atccctgatg
    33901 attcaccgat agtggggcat tcgccggttg ccatttagcg aaatacgaca gcgggatcgc
    33961 tttgccttcg ttattgataa cgaacatttt ttccagcgca ctgatgtcct gggtatagcg
    34021 cggatccact tccatcacca ctttatactg gttcatcggc tggtaaatgg tcgagatttg
    34081 ccgctgaccg aaggcgttat ttaacagact gttggcggcc tgtacgtcga ttcccagccg
    34141 tgccatggtg tcgcggtcgt aaaccagatt catctccgcg ccgttatcct gctgatcgga
    34201 gttcacgtcc gccagttccg gcaacgtcgc cagttttttg cggattttcg gctcccattc
    34261 gcgcagtgcc gccaggtcgt cggataacaa cgtgtactgg tagctggcgt tcgactgacg
    34321 cccaccaacg cgaatatctt gtaccgccat caggaacaga ttcgcccccg gttctttcgc
    34381 cagcttcacg cgcagacggt cgataatttg ctgcgccgtt tcgctgcgtt cgtcgcgtgg
    34441 cttgagggtg ataaacatca tcccactgtt aactcgtgaa ccgccggtaa agccggtgac
    34501 attatccact gccggatcgt cacggataat tttcatgaaa tcctgcaatt tgccgcgcat
    34561 cgcctgaaac gaaatactct gatccgcctg tatcccaccc atcagtacgc cggtgtcctg
    34621 ctccgggaag aaggttttcg ggatcgagat attcagcgca atggtgccaa gcagcaccac
    34681 gcccaccaga cgggtatgat tgagcaccca tttcagcgac ttgccgtagc cttgttgcag
    34741 ggctaccaac atgcggccaa aaccacgcag tcgcttttgc tcacgcggct tgctggcttt
    34801 tagcatccag ccgcacatca ttggcgttag cgtcagggaa accagcaacg aaataccaat
    34861 ggcgacagaa agcgtcacgg caaactcgcg taacagtcgg cccggcaatc cgcccatcaa
    34921 cagcagcggc aggaacaccg ccaccagtga cagactcatc gacagcaccg taaagccgac
    34981 ttcgcgagta ccttgcagcg cggcctgtag tggtttcatc cccgcttcca gatgacgggc
    35041 aatgttttcc agcaccacaa tggcgtcatc caccacgaaa ccagtagcaa tggtgagcgc
    35101 catcagcgaa agattattga gactgaatcc gcacaggtac atcgccgcaa acgtaccaat
    35161 cagcgaaacc ggcaccgcaa cggcgggaat aatagtggcg cgacccgagc gcaggaataa
    35221 aaagaccacc agaatcacca gcgccaccga gataatcagc gtttgctcga cttcttccag
    35281 cgaggcgcga atggtggggg agcgatcctg ggcaatttgc agatcaatcg ccgccggaat
    35341 ggtctcctgc aactctggta attttgcccg gatgctgtct accgtctgga taatattggc
    35401 ttccggcagt ttgcggatca tcagtaaaat agccggtttg gcgttggtca tcccggcgtt
    35461 gcgcacatcc tgcactgagt cagtcaccgt cgccacatcg cccagacgaa ccgcgccgcc
    35521 gttgttgtag tgaataatca gcggctgata ttcagcggcg gttttcagtt catcgttggt
    35581 ctggatctgc cagcggtgag tgccatcttc cagcgccccc tgcggtttac gcacgttggc
    35641 attgctgatg gcagtgcgta cgtcgtccag cgacacgccc tgattaaaca gcgcctgcgg
    35701 ggtcagcccg acgcgcacgg cgggcagcga gctgccaccg acatcgacat caccgacacc
    35761 gtcgatttgc gaaatcgtcg gagccagctg cgtcgaggcg aaatcgtaca gttcaccctg
    35821 cgaataagta tcggatgtca gcgtgaggat cataattggc gcatccgacg ggttcgcttt
    35881 gcgataggtc gggcggctgg gcatcccgct gggcagcaaa ctttgtgcag cgttgatcgc
    35941 cgcctgcaca tcacgcgctg cgccgttgat atcccggtca aaatcaaact gcaaaataat
    36001 gcgcgtgctg ccgagcgaac tgctggaggt catttcacta accccggcaa tgcgcccaag
    36061 tgagcgctcc agcggcgtgg caacggaaga cgccattgtc tccggcgacg caccgggcag
    36121 cgaggcgctg accataatca ccggaaaatc gacctgcggc agcggggcga ccggcagcat
    36181 acggaaaccc agtatgccgc acagggtaat ggcgaccgac agtaaaatcg tcgccaccgg
    36241 gcggtaaatg aagagggcaa aaaacttcac ttacgcctcc tcttcatggc gagcaaagcg
    36301 acttttggtc cacaatgcca ggcggtcgaa cagcagataa atcaccggcg tggtgaacag
    36361 cgtcagcacc tggctgacaa tcagaccgcc gaccatgccg atacctaacg gacgacgcag
    36421 ttccgcgccg accccggtac tcaacatcag cggcagcgcg ccaagcagag ccgccagggt
    36481 ggtcatcagg atcggacgaa aacgcaacag acaagcctgg tagattgcat cgcgcggcga
    36541 catgccttgc tcgcgctcag cagccagcgc gaagtcgatc atcatgatgg cgttcttctt
    36601 cacaataccg atcagcaaaa taatgccgat aatcgcaatc acatccagtt cgctaccggc
    36661 aatcattaat gccagcagcg cgccgacccc tgcggtgggt agcgtcgaga gaatggtgat
    36721 cgggtgaata aagctctcgt acagaatgcc gagcacgata tacatcgcca ccaccgccgc
    36781 gacaatcagc cagacagtgc tgcccagcgc cgactggaag gcgagggtgc tgccctagaa
    36841 ttgcgtggtg atatccaccg gcagattcag cgtcttttcg gtgtccataa tcgcctgcac
    36901 cgcatcgccc agcgaatagt tatccggcac gttaaaggag atggttgtta ccgggaactg
    36961 atccagatgg ttgatggaaa gcggcgcaaa acgctgctca attttggcaa ttgagcttaa
    37021 cggcaccacg ccaccgtcgc tgctggtcag gcgaatggta tccagcgccg cgaggcctgg
    37081 ggtattttcg gtattgtgct ccagcaccac gcgatactgg ttagcctgag tataaatagt
    37141 ggaaatcagt cgctgaccaa acgcgttgta cagggcgtta tcgacgtccg ccatgctgat
    37201 ccccagacgg ctggcgctgt cgcgatcaac attgacatac gccaccagcc ctttgtcctg
    37261 ccagtcgctg gagacatcag aaagctgtgg cagttgctgg agtttttcca tcaactgtgg
    37321 cacccaggta ctgagcgcat ccagtgacgt ggcctgcaag gtaaactggt actgggtgcg
    37381 gctgacctga gtatcgatgg tcaggtcctg cgttggttgc aggaagagat cgacgcccgg
    37441 cactttatct accgccgttt gcagacgggc gatgactttt tgcacccgat catcacgttc
    37501 atccaacggt ttgaggttga tttgtaaacg tgcactattc agcgacgggt tagtgccatc
    37561 aacgccaaca aatgaggtca ggctttgcac tgccggatcc tgcaaaatca cgtccgcgac
    37621 ctggcgttgt cgctgggcca tattggcaaa ggagctggat tgcggtgcct gcaaagtgcc
    37681 ctgaataatg ccgttgtcct gtaccgggaa gaaacctttc ggaatgaaca cccacagcag
    37741 cacgctaagc agcagcgtgc tgagtgccac gcttaaggtc agccacggat gattcagcac
    37801 tttcgccagt ccacgaccat aggcggcgat tatcctgtcg aacatttttt ccgaggcacg
    37861 ggagaagcgg ttctgtttac gcaacgactc ctggctgagc atccgcgcgc acatcatcgg
    37921 tgtcagggtc agcgacacca ccgctgagat caaaatcgct accgccaggg taatagcaaa
    37981 ttcgcggaac agtcgcccga cgatatcgcc cataaacagc agtgggatca acaccgcaat
    38041 cagtgagaag gtcagcgaga taatggtaaa gccgatttca cctgcgccct tgagcgccgc
    38101 cgccaacggt ttttcgcctt tttcgatata gcgggaaatg ttttcgatca ccacgatggc
    38161 gtcatcgacc acgaatccgg tggcgatagt taacgccatc agtgtcaggt tattgattga
    38221 aaaatcgaga aacaccataa ccgcgaaagt gccgattaac gacagcggta cagcaacacc
    38281 gggaatgatg gtcgccggaa tattgcgcaa aaacaggtag ataatcatga ctaccagcgc
    38341 gatagccatc atcaattcaa actgagtatc atcgacggat gcgcggatat tggtggtgcg
    38401 atcggaaagc actgtcacct tcaccgattt cggcagactc tcagtgagct gtggcagcat
    38461 ctgccgaatg ctgtcggcgg tggagataat gttagcaccg ggctggcgct gaacattcat
    38521 cacaatggcc tgttctttgt tcgcccacgc gccgagccag ctgttttctg caccttgctc
    38581 tacagttgcg acatcgccca gacgaattgg cgcgccgttc tggtaggcga tgattagctg
    38641 gcgatactct tcggcggatt gcatctggtc gttcgcggaa agcgtgaccg cacgggaagg
    38701 gccgtcgagg ctaccttttg ccgagttaac gttagcgccg gtaatggcgg tgcgcacggt
    38761 ttcgctggtc aggccgaggg cggcaatcgc ctgagcgtta agtttgacgc gaacagccgg
    38821 acgctgaccg ccggaaagcg tcaccaggcc gacgccggaa atctgcgaga ttttctgcgc
    38881 gacgcgggtt tccaccatat cttccacttg cgtcatcggc atggcggttg aggtgacggc
    38941 gagcgtcatg atcggcggat ctgccgggtt cactttgctg taaaccggcg ggttaggcag
    39001 atcgctcggc aacaagttgg tcgcagcgtt aatcgcggcc tgcacttcct gctcggcgac
    39061 attgagcggt aatgttagct ggaactgcaa agtgataact gacgcaccgc cggaactttg
    39121 cgacgacatc tgtttcaggc cagacatctg cccgaactgg cgttcgagcg gcgcggtaac
    39181 ggcagaggtc atgacatccg ggctggcacc tgggtagagc gtgaccacct gaatggtcgg
    39241 atagtccact tccggcagcg ccgaaacggg cagggcgcga taaccgataa tcccggcgag
    39301 taagatcgcc accatcagca gcgtggtggc gacaggacgc ataataaaca ggcgcgacgg
    39361 gccgcctgtg ctgctcgggg gtaacacctg catcaggagc gtgctccttt tttcgcgtat
    39421 tcgcggctgg tggctttctc ttccggagtg gtggcgctct gggcttccac cacttccact
    39481 ttcgcccctt cggtcaggcg atcaatgccg tctgtcacca cgcgatcgcc cgcagaaata
    39541 cctgcacgga tcaccacttt ctgactgtcc tgaatgcccg gcgtcaccag atgtttgctg
    39601 accttgtttt cgctattcag cacccagaca aaatggcctt cattgcccat ttgcagggcg
    39661 gctgttggga tcactacggc gttttgttcg gtgtcgacta acatgcgcgc gttaacaaac
    39721 tgattgggaa acagcgcatc atcctgatta ttaaagcgtg ctttcacttt aatcgtaccg
    39781 gttgtggcat cgatttggtt atcgaggctt aacagcgtgc cttcacttaa tttcttcgaa
    39841 ttggtgcgat cccaggcttc taccatcagc ggttttccgg ctttttgcgc ctgcactaca
    39901 gtagcgatat cgctttccgg cagggtaaag actaaatcga taggatgcgt ctgggtgatc
    39961 accacaattc cggtggtatc accactggag atttggttac caacatcaac ttgcttgaga
    40021 ccaacgcgac catcgactgg tgcggtgatg cggctccagt cgagttgcaa ctgcgcgctg
    40081 gcgacgcttg ct
//
LOCUS       DAJVPP010000028        39150 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVPP010000028 DAJVPP010000000
VERSION     DAJVPP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.028"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            29..619
                     /gene="uidR"
                     /locus_tag="OQM34_001431"
     CDS             29..619
                     /gene="uidR"
                     /locus_tag="OQM34_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1451935.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1007..2710
                     /gene="uidA"
                     /locus_tag="OQM34_001432"
     CDS             1007..2710
                     /gene="uidA"
                     /locus_tag="OQM34_001432"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS1451936.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            2716..3741
                     /gene="uidB"
                     /locus_tag="OQM34_001433"
                     /pseudo
     CDS             2716..3741
                     /gene="uidB"
                     /locus_tag="OQM34_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            3780..5045
                     /gene="uidC"
                     /locus_tag="OQM34_001434"
     CDS             3780..5045
                     /gene="uidC"
                     /locus_tag="OQM34_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1451937.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5271..6779)
                     /locus_tag="OQM34_001435"
     CDS             complement(5271..6779)
                     /locus_tag="OQM34_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1451938.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(6880..8055)
                     /gene="manA"
                     /locus_tag="OQM34_001436"
     CDS             complement(6880..8055)
                     /gene="manA"
                     /locus_tag="OQM34_001436"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1451939.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8254..9900
                     /gene="fumB"
                     /locus_tag="OQM34_001437"
     CDS             8254..9900
                     /gene="fumB"
                     /locus_tag="OQM34_001437"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1451940.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10043..11446
                     /gene="fumC"
                     /locus_tag="OQM34_001438"
     CDS             10043..11446
                     /gene="fumC"
                     /locus_tag="OQM34_001438"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1451941.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11443..12372)
                     /gene="tus"
                     /locus_tag="OQM34_001439"
     CDS             complement(11443..12372)
                     /gene="tus"
                     /locus_tag="OQM34_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1451942.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12448..13749)
                     /gene="rstB"
                     /locus_tag="OQM34_001440"
     CDS             complement(12448..13749)
                     /gene="rstB"
                     /locus_tag="OQM34_001440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1451943.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVSPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(13928..15247)
                     /locus_tag="OQM34_001441"
     CDS             complement(13928..15247)
                     /locus_tag="OQM34_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCS1451944.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15472..16215)
                     /gene="rstA"
                     /locus_tag="OQM34_001442"
     CDS             complement(15472..16215)
                     /gene="rstA"
                     /locus_tag="OQM34_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS1451945.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16344..16679
                     /locus_tag="OQM34_001443"
     CDS             16344..16679
                     /locus_tag="OQM34_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS1451946.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPTAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(16676..17398)
                     /gene="folM"
                     /locus_tag="OQM34_001444"
     CDS             complement(16676..17398)
                     /gene="folM"
                     /locus_tag="OQM34_001444"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1451947.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17435..18817)
                     /locus_tag="OQM34_001445"
     CDS             complement(17435..18817)
                     /locus_tag="OQM34_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1451948.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19003..19947)
                     /locus_tag="OQM34_001446"
     CDS             complement(19003..19947)
                     /locus_tag="OQM34_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1451949.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20471..22003
                     /gene="pntA"
                     /locus_tag="OQM34_001447"
     CDS             20471..22003
                     /gene="pntA"
                     /locus_tag="OQM34_001447"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1451950.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22014..23402
                     /gene="pntB"
                     /locus_tag="OQM34_001448"
     CDS             22014..23402
                     /gene="pntB"
                     /locus_tag="OQM34_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1451951.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23427..24461)
                     /gene="tqsA"
                     /locus_tag="OQM34_001449"
     CDS             complement(23427..24461)
                     /gene="tqsA"
                     /locus_tag="OQM34_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS1451952.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            24872..25237
                     /gene="mdtJ"
                     /locus_tag="OQM34_001450"
     CDS             24872..25237
                     /gene="mdtJ"
                     /locus_tag="OQM34_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1451953.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25224..25553
                     /gene="mdtI"
                     /locus_tag="OQM34_001451"
     CDS             25224..25553
                     /gene="mdtI"
                     /locus_tag="OQM34_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1451954.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25592..26413)
                     /locus_tag="OQM34_001452"
     CDS             complement(25592..26413)
                     /locus_tag="OQM34_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1451955.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26513..26596)
                     /locus_tag="OQM34_001453"
     CDS             complement(26513..26596)
                     /locus_tag="OQM34_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451956.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(26689..27024)
                     /locus_tag="OQM34_001454"
     CDS             complement(26689..27024)
                     /locus_tag="OQM34_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCS1451957.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27421..28674)
                     /locus_tag="OQM34_001455"
                     /pseudo
     CDS             complement(27421..28674)
                     /locus_tag="OQM34_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            28781..29683
                     /locus_tag="OQM34_001456"
     CDS             28781..29683
                     /locus_tag="OQM34_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1451958.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            29818..31038
                     /gene="mlc"
                     /locus_tag="OQM34_001457"
                     /gene_synonym="dgsA"
     CDS             29818..31038
                     /gene="mlc"
                     /locus_tag="OQM34_001457"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1451959.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31163..31858
                     /gene="bioD"
                     /locus_tag="OQM34_001458"
     CDS             31163..31858
                     /gene="bioD"
                     /locus_tag="OQM34_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1451960.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(31811..33103)
                     /gene="clcB"
                     /locus_tag="OQM34_001459"
     CDS             complement(31811..33103)
                     /gene="clcB"
                     /locus_tag="OQM34_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1451961.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33262..33876)
                     /gene="dmsD"
                     /locus_tag="OQM34_001460"
     CDS             complement(33262..33876)
                     /gene="dmsD"
                     /locus_tag="OQM34_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1451962.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(33919..34773)
                     /locus_tag="OQM34_001461"
     CDS             complement(33919..34773)
                     /locus_tag="OQM34_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1451963.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(34775..35392)
                     /gene="dmsB"
                     /locus_tag="OQM34_001462"
     CDS             complement(34775..35392)
                     /gene="dmsB"
                     /locus_tag="OQM34_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1451964.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35403..37441)
                     /locus_tag="OQM34_001463"
                     /pseudo
     CDS             complement(35403..37441)
                     /locus_tag="OQM34_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37502..38611)
                     /locus_tag="OQM34_001464"
     CDS             complement(37502..38611)
                     /locus_tag="OQM34_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1451965.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADTLLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38594..>39150
                     /locus_tag="OQM34_001465"
     CDS             38594..>39150
                     /locus_tag="OQM34_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1451966.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDG"
BASE COUNT         9947 a         9491 c        10033 g         9679 t
ORIGIN      
        1 agttattcac gctaactata atggtttaat gatggataac atgcagactg aagcacaacc
       61 gacacggacc cggatcctca atgctgccag agagattttt tcagaaaatg gatttcacag
      121 tgcctcgatg aaagccatct gtaaatcttg cgccattagt cctgggacgc tctatcacca
      181 tttcatctcc aaagaagcct tgattcaggc gattatctta caggaccagg agagggcgct
      241 ggctcgtttc cgggaaccga ttgaagggat tcattttgtt gactatatgg tcgagtctat
      301 tgtctctctc acccatgaag cctttgggca acgggcgctg gtggtcgaaa ttatggcgga
      361 agggatgcgt aacccacagg tcgccgccat gcttaaaaat aagcatatga cgatcactga
      421 atttgttgcc cagcggatgc gtgatgccca gcaaaaaggc gagataagcc cagacatcaa
      481 cacggcaatg acttcacgtt tactgctgga tctgacctac ggtgtactgg ccgatatcga
      541 agcggaagac ctggcgcgtg aagcgtcgtt tgctcaggga ttacgcgcga tgattggcgg
      601 tatattaacc gcatcctgat cctctcattt cgcgggctgg tgataactgt gcccgcgttt
      661 catatcgtaa tttctctgtg caaaaattat cctccccgtg ttcggagaat tcccccccaa
      721 aatattcacc gtagccatat gtcatgagag tttatcgttc ccaacactct tgaacgaacg
      781 ttcgattgct tattttatgg cttctgtcaa cgctgtttta aagattaatg cgatccatat
      841 cacgctgtgg gtattgcagt ttttggtttt ttgatcgcgg tgtcagttct ttttatttcc
      901 atttctcttc catgggtttc tcacagataa ctgtgtgcaa cacagaattg gttaactaat
      961 cagattaaag gttgaccagt atttttatct taatgaggag tcccttatgt tacgtcctgt
     1021 agaaacccca acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg
     1081 cgaaaactgt ggaattgatc agcgttggtg cgaaagcgcg ttacaagaaa gccgggcaat
     1141 tgcagtgcca ggcagtttta acgatcagtt cgccgatgca gatattcgta attatgtggg
     1201 caacgtctgg tatcagcgcg aagtctttat accgaaaggt tgggcaggcc agcgtatcgt
     1261 gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg gtcaataatc aggaagtgat
     1321 ggagcatcag ggcggctata cgccatttga agccgatgtc acgccgtatg ttattgccgg
     1381 gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg aactggcaga ctatcccgcc
     1441 gggaatggtg attaccgacg aaaacggcaa gaaaaagcag tcttacttcc atgatttctt
     1501 taactatgcc gggatccatc gcagcgtaat gctctacacc acgccgaaca cctgggtgga
     1561 cgatatcacc gtggtgacgc atgtcgcgca agactgtaac cacgcgtctg ttgactggca
     1621 ggtggtggcc aatggtgata tcagcgttga actgcgtgat gcggatcaac aggtggttgc
     1681 aactggacaa ggcaccagcg ggactttgca agtggtgaat ccgcacctct ggcaaccggg
     1741 tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc cagacagagt gtgatatcta
     1801 cccgctgcgc gtcggcatcc ggtcagtggc agtgaagggc gaacagttcc tgattaacca
     1861 caaaccgttc tactttactg gctttggtcg tcatgaagat gcggatttgc gaggcaaagg
     1921 attcgataac gtgctgatgg tgcacgatca cgcattaatg gactggattg gggccaactc
     1981 ctaccgtacc tcgcattacc cttacgctga agagatgctc gactgggcag atgaacatgg
     2041 catcgtggtg attgatgaaa ctgcagctgt cggctttaac ctctctttag gcattggttt
     2101 cgaagcgggc aacaagccga aagaactgta cagcgaagag gctgtcaacg gggaaactca
     2161 gcaggcgcac ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt
     2221 ggtgatgtgg agtattgcca acgaaccgga tacccgtccg caaggtgcac gggaatattt
     2281 cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa
     2341 tgtaatgttc tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct
     2401 gaaccgttat tacggttggt atgtccaaag cggcgatttg gaaacggcag agaaggtact
     2461 ggaaaaagaa ctgctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata
     2521 cggcgtggat acgttagccg gcctgcactc aatgtacacc gacatgtgga gtgaagagta
     2581 tcagtgtgca tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ccgtcgtcgg
     2641 tgaacaggta tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg
     2701 taacaattga cctacatggg cctcgggctt tgctacagct tggtgaatat tccttatggt
     2761 tcacttgcta ccgcgatgac ccaacaacca caatctcgcg cccgtctggg cgcggctcgt
     2821 gggattgccg cttcattgac ctttgtatgc ctggcatttc tgataggacc gagcattaag
     2881 aactccagcc cggaagagat ggtgtcggta taccatttct ggacgattgt gctggtgatt
     2941 gccggaatgg tgctttactt catctgcttc aaatcgacgc gtgaaaatgt ggtacgtatc
     3001 gtggcgcagc cgtcattgaa gatcagtttg caaaccctga aacggaatcg cccgctgttt
     3061 atgttgtgca tcggtgcgct gtgtgtgctg atttcgacct tcgcggtcag cgcctcgtcg
     3121 ttgttctacg tgcgctatgt gttaaatgat accgggctgt tcactgtgct ggtactggtg
     3181 caaaacctgg ttggtactgt ggcatcggca ccgcttgtgc cggggatggt cgcgaggatc
     3241 ggtaaaaaga ataccttcct gattggcgct ttgctgggaa cctgcggtta tctgctattc
     3301 ttctgggttt ccgtctggtc actgccggtg gcgttggttg cgttggccat cgcttcaatt
     3361 ggtcagggcg ttactatgac tgtgatgtgg gcactggaag ctgataccgt agaatacggt
     3421 gaatacctga ccggcgtgcg aattgaaggg ctcacctatt cactattctc atttacccgt
     3481 aaatgcggtc aggcaatcgg aggttcaatt cctgccttta ttttgggatt aagcggatat
     3541 atcgccaatc aggtgtaaac gccggaagtt attatgggca tccgcacatc aattgcctta
     3601 gtaccttgcg gatttatgct actggcattc gttattatct ggttttatcc gctcacggat
     3661 aaaaaattca aagaaatcgt ggttgaaatt gataatcgta aaaaagtgca gcagcaatta
     3721 atcagcgata tcactaatta atattcaata aaaataatca gaacatcaaa ggtgcaacta
     3781 tgagaaaaat agtggccatg gccgttattt gcctgacggc tgcctctggc cttacctctg
     3841 cttacgcggc gcaactggct gacgatgaag cgggactacg catcagactg aaaaacgaat
     3901 tgcgcagggc tgataagccc agtgctggcg cgggaagaga tatttacgca tgggtacagg
     3961 gaggattgct cgatttcaat agtggttatt attccaatat tgttggcgtt gaaggcggag
     4021 cgtattatgt ttataaatta ggtgctcgtg ctgatatgag tacccggtgg tatcttgatg
     4081 gtgataaaag ttttggcttt gccctggggg cagtaaaaat aaaacccagt gaaaatagcc
     4141 tgcttaaatt aggtcgcttc gggacggatt atagttatgg tagcttacct tatcgtattc
     4201 cgttaatggt tggcagttcg caacgtacat taccgacagt ttctgaagga gcattaggtt
     4261 attgggcttt aacgccaaat attgatctgt ggggaatgtg gcgttcacga gtatttttat
     4321 ggactgattc aacaaccggt attcgtgatg aaggggtgta taacagccag acgggaaaat
     4381 acgataaaca tcgcgcacgt tcttttttag ccgccagttg gcatgatgat accagtcgct
     4441 attctctggg ggcatcggta cagaaagatg tttccaatca gatacaaagt attctcgaga
     4501 aaagcatacc gctcgacccg aattatacgt tgaaagggga gttgctcggc ttttacgcgc
     4561 agctcgaagg tttaagtcgt aataccagcc agcccaatga aacggcgttg gttagtggac
     4621 aattgacctg gaatgcgccg tggggaagcg tatttggcag tggtggttat ttgcgccatg
     4681 caatgaatgg tgccgtggtg gataccgaca ttggctatcc cttttcatta agtcttgatc
     4741 gtaaccgtga aggaatgcag tcctggcaat taggtgccaa ctaccgtgta actccgcaat
     4801 ttacgctgac atttgcaccg attgtgactc gcggctatga atccagtaaa cgagatgtgc
     4861 ggattgaagg cgcgggtatc ttaggtggta tgaactatcg ggtcagcgaa gggccgttac
     4921 aagggatgaa tttctttctt gctgccgata aagggcggga aaagcgcgat ggcagtacgc
     4981 tgggcgatcg cctgaattac tgggatgtga aaatgagtat tcagtatgac tttatgctga
     5041 agtaagaaat aacgccggag agaaaaacct ccggcgtttc agattgttga caaagtgcgc
     5101 gttttttatg ccggatgcgg cgtaaacgcc ttatccagcc tacaaaaact cataaattca
     5161 aagtgttgca ggaaaaggta ggcctgataa gcgtagcgca tcaggcaatc tctggtttgt
     5221 tttcagatga aaacgccgga gtgaaaattc tccggcgttt tggccgtgaa ttactgctgc
     5281 ggaattgccg gtacagccgg aacgttaaga gctggcatcg caaacatgcc aacaaaatct
     5341 tctaacgaca ttttctgccc atttaacgtt atctgaccgt tagcatattg caggctggtg
     5401 gtgatggtat tgtcctgcaa ggtggtcaga cggaacatct gccccattgc tgatgcacct
     5461 tcaacttgct gtttcgccag ttttttcgct tgatcttcct gataaccttc cagcttcgct
     5521 acctgagtca tcaactcagt tgccatatcc accggaatgg tcagtttcgc atccagagat
     5581 ttaaccgaac gatctacttc ctgcgccagc gtttgcggcg cttctttagt cgttgccgga
     5641 tctttcagga acagcgacag attcagcgca ctttcaccct gactgttttt ccagcttagc
     5701 ggcgcgatag taatcaccgg atcgcctttc aacatcagcg gcagggcgct aaagaaggct
     5761 tccgtcactt tctcctgata aagttcggga ttgttggcaa tttctggctg tgccagcagc
     5821 gcctgagttt gcgcgttata ttgctggcta aactgatgcc aggcttcacc atcaatctgg
     5881 ccgactttta aagtcagctt gccgctgccc agatcctgat tctgtacctt caggctgttt
     5941 agcgagtaat ccagttggct attgatcgtt ttaccgtcat tgaccagatc cgatttaccg
     6001 ctgatctcca tgccttccag cagtgccagt tctttgcctt ccactgaaat ggtcattttt
     6061 tccagtgaca gtttttggtt tcctacgcgc tcaccaaaac ttcccagcgt gctggaaccg
     6121 tcggttttca gattattaaa ggtcaactgc actttctggt tgtattcgtt aactgcgtct
     6181 atccgaccac tttgcgcctc tccggaaagg gagatggctt tgccgtctct gtcagcattt
     6241 aactggaact cgccgccgct aaaggcgact ttttcatcct tttgctcgta attcagtgga
     6301 ttgagcgaaa tatcggaact ggaatcaccg ctgtaaccaa tgcgcgagtt aatctcaaaa
     6361 ggcgtttcac cttttgccat atcaaacagt ggtttgctta cttcgttatt aaccagcgtg
     6421 gtttgaattg atgccatcga cgggatcagg ttcagttttt taagctgggc aagcgggaag
     6481 ggaccatgat caaccgattc gttgaagatg acgctctgac cgcttttaat ccacggattt
     6541 tctttcccgg caatgggttt caccaacagt tgcaactggc tgctgaatac gccgcgatga
     6601 tagttttgat aactcacttc caggttggat tcaggagctg tcagtttgag ttgcgcgttc
     6661 gcctgcgcga ccatgtcttc gagatgggtt tcaatcttct tgcctgtata ccatgcgccg
     6721 cctgtccaga ctacgcctag cgcaacaatg acgcctaccg ctaccagcga tttattcata
     6781 atgattatcc ataaaatgaa atcaggcgga ctggccgcct gaaggtgtta taagccttta
     6841 ataagcttag caagagatgt taattttttc agtaagctct tacagcttgt tgtaaacacg
     6901 cgctaaacgg ccgtggcctt tgacagtcac cggtgattcg ttggcggcaa taaacgctga
     6961 ttcacccggt ttaagctgta actgctgaga acctttccac aacgttgcat cgccttcgac
     7021 gcagaacaaa atggcggcac tctgctggct gatggtggtt tctttatcac taaggtcatg
     7081 cagcgagaag gcaaaatcat ccacaggaat cgggaagtcc agttctgcac cttgtttcac
     7141 cggctgggtc aacaactggt tagccggttt ggcttcgaat ttcacattgg caaccagttc
     7201 cggaatatca atgtatttag gcgtcagacc tgcacgcagc acgttatcgg agttcgccat
     7261 cacttccagc gccacgcctt gcaggtaagc gtgcggtgtt tcagcgaaca ggaacatcgc
     7321 ttcgccagga ttcaatttca ccacattcag taacagcggg gagaacaggc cgctgtcttc
     7381 cgggtaaaat tcagaaatta aacgaatcgt ttgccacggt tcaccctgct gactgtcgag
     7441 cgccgatttt aaaatcgcca gcgcgcggga tttttcttca ccctgcatat tcaacaggtt
     7501 ggcgaacagt tcgcttaaac gttcggcatg tggctgttgt aaaaagtgag caatcgccgg
     7561 atgtgcaccg gcgaccggct ggagtaggga gacaatctcg gaaaattcac gaaacgcgtt
     7621 catcgcaagg aaaggcgtca gcgcaaaaac cagctccggc ttgtggttag gatctttata
     7681 gttacgctcg gcggcatcca tcgggatacc tgcggcattt tctttggcaa aaccgatttc
     7741 agaattgtgt ttgtttggat gaacctgaat ggagagtggc tgtgctgcgc ataatacttt
     7801 gaacaggaaa ggcagttcgc caaagcgttt ggcaacggcc tctccgagca gagtcgattt
     7861 atcactctca atcacatcac gcagtgaaac gatatctccg gcggcattct gcactcgtga
     7921 actgcttttc ggatgtgcgc ccatccacag ctcggccatc ggctggctgg acggattttc
     7981 cataccataa agttcagtca acgccgtttt gctaccccag gcatagtttt gcactgagtt
     8041 aatgagtttt tgcatgatca atccctgttt taatgtggaa attaatcccg ctattaaagc
     8101 aagaatccta cgggaagtaa cctggagccg caaaaagtcg tactagtctc agtttttgtt
     8161 aaaaaactgt gtaggatatt gttactcgct tttaacaggg caacggaaca cccgcccaga
     8221 gcataaccaa accaggcagt aagtgagaga acaatgtcaa acaaaccctt tcattatcag
     8281 tctccttttc cactcaaaaa aaatgatact gagtattacc tgctaaccag tgaacacgtt
     8341 agcgtatctg aatttgaagg gcaggagatt ttgaaagtcg cacccgaagc gttaactctg
     8401 ttggcgcgtc aggcgtttca tgatgcgtcg tttatgctgc gtccggctca ccaacaacag
     8461 gtggccgaca ttctgcgtga cccggaggcc agcgaaaatg ataaatatgt ggcgctgcaa
     8521 ttcctgcgta actccgacat cgcggcgaaa ggcgttctgc caacctgtca ggataccggc
     8581 accgcgatta ttgttggtaa aaaagggcag cgtgtatgga ccggtggtgg tgatgaagcg
     8641 gcgctggcgc gcggtgtcta taacacttat atcgaagata atctgcgcta ctcgcaaaac
     8701 gcgccgctgg atatgtataa agaagtgaat accggcacca atctgccagc gcagatcgat
     8761 ctttatgccg ttgatggcga cgaatacaaa ttcctctgta tcgcaaaagg tggtggttcg
     8821 gcaaacaaga cgtatctcta tcaggaaacc aaagcgttac tgacgccggg gaaactgaaa
     8881 aattacctgg ttgagaagat gcgcacgctg ggtaccgcgg cctgtcctcc gtatcatatt
     8941 gcgttcgtta ttggtggaac ttctgcagaa acgaacctta aaacggtgaa actggcttcc
     9001 gcgaaatact atgatgaact gccaacggaa gggaatgagc acggtcaggc gttccgcgat
     9061 gtggaactgg aaaaagaatt actgatcgaa gcgcaaaatc tcggtctggg tgcgcagttt
     9121 ggtggtaaat acttcgctca cgacatccgc gtgattcgcc tgccacgtca cggcgcatcc
     9181 tgtccggttg gtatgggtgt ttcctgctct gctgaccgta atatcaaagc gaagatcaac
     9241 cgtcagggga tctggatcga aaaactggaa cataatccag gtaaatatat ccctgaagag
     9301 ctgcgtaaag cgggagaagg cgaagcggtg cgcgttgacc ttaaccgtcc gatgaaagag
     9361 atcctcgcac agttgtcgca gtatcccgtt tctacacgct tatcgcttaa cggcacgatt
     9421 atcgttggtc gtgatattgc tcacgccaaa ctgaaagagc ggatggataa cggtgaaggg
     9481 ctgccgcaat acattaaaga tcatccgatt tactacgcgg gtccggccaa aacgccggaa
     9541 ggttatgcct ccggttctct tggcccaacg accgccggac ggatggattc ttatgtcgat
     9601 caactgcaag cgcagggcgg aagtatgatc atgctggcga aaggcaaccg cagccagcag
     9661 gtgacggatg cctgtaaaaa acacggcggc ttctaccttg gcagtatcgg tggtccggcc
     9721 gctgtattgg cgcagggaag tattaagagc ctggaatgtg ttgaatatcc ggaactggga
     9781 atggaagcca tctggaaaat tgaagtggaa gatttcccgg cgtttatcct tgtggatgat
     9841 aaaggaaatg acttcttcca gcagatacaa ctcacacagt gcactcgctg tgtgaaataa
     9901 acagagccgc ccttcggggc ggttttttta catggcacga aagaccaaac atttgttatc
     9961 aaatagtaaa taatgagtga gctaaaagtt gcttaacgaa agcaaaacag aaagaaaaaa
    10021 ttaatcaggt gaggagcagg ccatgaatac agtacgcagc gaaaaagatt cgatgggggc
    10081 gattgatgtc ccggcagata agctgtgggg cgcacaaact caacgctcgc tggagcattt
    10141 ccgcatttcg acggagaaaa tgcccacctc actgattcat gcgctggcgc taaccaagcg
    10201 cgcagcggca aaagttaatg aagatttaga cttgttgtct gaagagaaag cgagcgccat
    10261 tcggcaggcg gcggatgaag tactggcagg acagcatgac gacgaattcc cgctggctat
    10321 ctggcagacc ggctccggca cgcaaagtaa catgaacatg aacgaagtgc tggctaaccg
    10381 ggccagtgaa ttactcggcg gtgtgcgcgg gatggaacgt aaagttcacc ctaacgacga
    10441 cgtgaacaaa agccaaagtt ccaacgatgt ctttccgacg gcgatgcacg ttgcggcgct
    10501 gctggcactg cgcaagcaac tcattccgca gcttaaaacc ctgacacaga cgctgagtga
    10561 aaaatcgcgt gcatttgccg atatcgtcaa aatcggtcga acccacttgc aggacgccac
    10621 gccgctaaca ctagggcagg agatttccgg ctgggtagcg atgctcgagc ataatctcaa
    10681 acatatcgaa tacagcctgc ctcacgtagc ggaactggct cttggcggta cagcggtggg
    10741 tactggacta aatacccatc cggagtatgc gcgtcgcgta gcagatgaac tggcagtcat
    10801 tacctgtgca ccgtttgtta ccgcgccgaa caaatttgaa gcgcttgcga cctgtgatgc
    10861 cctggttcag gcgcacggcg cgttgaaagg gttggctgcg tcactgatga aaatcgccaa
    10921 tgatgtccgc tggctggcct ctggcccgcg ctgcggaatt ggtgaaatct caatcccgga
    10981 aaatgagccg ggcagctcaa tcatgccggg gaaagtgaac ccaacccagt gtgaagcatt
    11041 aaccatgctc tgctgtcagg taatggggaa cgacgtggcg atcaacatgg gtggcgcttc
    11101 cggtaacttt gaactgaacg tcttccgtcc gatggtgatc cataatttcc tgcaatcggt
    11161 gcgcttgctg gcagatggca tggaaagttt taacaaacac tgcgcagtgg gtattgaacc
    11221 gaatcgtgag cgaatcaatc aattactcaa tgaatcgctg atgctggtga ctgcgcttaa
    11281 cacccacatt ggttatgaca aagccgcgga gatcgccaaa aaagcgcata aagaagggct
    11341 gaccttaaaa gctgcggccc ttgcgctggg gtatcttagc gaagccgagt ttgacagctg
    11401 ggtacggcca gaacagatgg tcggcagtat gaaagccggg cgttaatctg caacatacag
    11461 gtgcagccgt ggaatgatca aacgaagcgg ctgcgcctga ggtttataac ggtgctgcac
    11521 attgtcggca tcgtaattta acaactcacc aacgtccggc acgcccgcgc cattatcccg
    11581 attaatcagt gcaatcagtg gtgtagggca ggcgtgttgg acttgttttt gatctccttt
    11641 gtaccagacg cgggcaatcg gctgcacctt cactggacgt ttgattttta acttcgcgtt
    11701 ctgtggcagg gcagcgatat cctgatactc tcgctccagt tttctttgcc actcctcgcg
    11761 cgtccacggt gcgacactgc gtggtgattt caggcttttt tccagctgtg ccaggacttc
    11821 atcacgatgc aaattcttaa tgatatgttt attagcccaa ccaaagcgta aagtggcggg
    11881 gtcgtgcaga acggtgagcg tgcggtaagc attaagggtg atcagccccg gcaaatgacg
    11941 atgcacccat tcaaaacgtg ccgcggtggg gagttctgat tcaaccgtga cgatatgctc
    12001 gaacgtggtc ttgagtttat tgatgtgctg aatatgactg accaacgctg cttgcgaaag
    12061 gttatcgacc tggtaacaca acacgccagg cagacggaca gcggccttgc tgctgcgatt
    12121 ttcggactgt tgttgaataa ataaatggcg gaaatgacgc aacgccagcg actgcgcgtc
    12181 gttgccgaga tgttgtttta cctcaatacg attaagcgga ttatgctcat cctctttttt
    12241 tacctccggc aaagagaaca cgcgggcaac caatagcttg tgttgctcaa gatgagcggc
    12301 aaatgtagcc agctcttgtt ccatctggcg aaaggtagtg ttgagtcggt ctacgagatc
    12361 gtaacgcgcc ataatattaa ccactttagt tacaacatac ttattttata gcacagtcgt
    12421 ggtgacttga ccatcgtgcg cgtagtgtca ggcagaggta aattgcggga tgttatgcca
    12481 taacggccag ctaaacgaga agcgagcacc acccagttcg ctgatgtcac aattaaccgt
    12541 accgcccatt gccagtgcta ttgagtggac aattgccagc cccagcccgc agccgccggt
    12601 tgagcgatcc cggctgggat cgaggcgaac aaaaggttca aagatatgtt cgcggttttc
    12661 tggggcaatc cctgggccat catcctcaac aattaatgtc gctctattcc ccgacagtag
    12721 caggctggtt tcaaccgttg aatggcagta gcgcagggcg ttattgagca aattatccag
    12781 cacgcgctcc attaagcgca tatccaacgc cgcataatgg ccttgcatga gcgtttttat
    12841 ccgtaccgtt ttatcgggcg ttactgcctg aatatctgcc agatgcgttg acagccacaa
    12901 cggcaggtct ggttcgctaa gatgaagctc gttttgtggg cgatcgagtc gggcataagt
    12961 cagcagctct tcaattaaag cttcaagttg actgatatca cgattcaacg cctgggattc
    13021 ggcggcgctc aggttatcgc tcatctccag tcgataacgc aggcgcacta acggtgttcg
    13081 cagttcgtga gcgataccgt caataagctg ttttttgctg gcaattaagg cgttgatatt
    13141 gtccgccatc tggttaaatg cgataccaag tcgttcaaag ctcgaaccct catcaaagtg
    13201 gatacgttca ttgagatgcc catcgccaaa tcgttgcgcc gctgcttcca gttttaacat
    13261 atcctgccag tgcggacgca tccagataaa caccggaaag gcgagggaaa tagcaataaa
    13321 agcgatcagg gcgatatcca gcaatcgcat ttgatggagg taataaagat aaggaacagg
    13381 actaactgcc agcacgtagt ggctgcgcgg gatacgctgc aaaaacgtgt actgatcgtc
    13441 cagggcgaca atttcgccgc cacgcagtcg gtgcatggaa atatcatcaa gatggtattt
    13501 actcagtggc tcgacacgca gatcgaaaga gagatttaaa tccatctctt tcagcgtctt
    13561 accccagtcg tgtgggggga tctctcgcaa ttcgctgcgc atcagataca gcgaactgtt
    13621 catcaaatca tccagcgact gtttgcccgc gcgttcggcg gtaaatttgt acaccagccc
    13681 aaccagcaga gacatcacaa ggaagcagac aaacaataac aggtaaaact ggataaacaa
    13741 ttttttcatt ggcatcgccc gcaacagaat actattcgta ttaattcacg actgtggagc
    13801 aatgtaaacc gccaactgga taagaccttg tttggaagat aatgtccgga acagacggat
    13861 gtatccccgg tgtattcata ttcagtaaat tcaattttcc accggggctt gtttattatg
    13921 ctaaggttta atttaacgtg acgctttgtt gttctttagt ctctctatcc ttaagaagac
    13981 gggctgatat tacaccggac attattgaac cattcacatt aagcgctgtt cttgccatat
    14041 caacgatcgg ctctatggca ataaaaataa ctactaatgc gacagggaaa ttaagtgttg
    14101 aaaggacaat cagcgccgcg aatgtcccac cactacctac gcctgctacc cctattgagc
    14161 caagcgcgat ggcaggtaaa agagttgcga ggaattgcaa tgagaaggga tcgataccca
    14221 ttgttggagc aatcatcgcc accatcacgg caggataaat cccagcgcag ccattttgtc
    14281 ccatgctgga accaaaagac gctgcaatat tagcaatcgt atttggaacc cccaattttt
    14341 ccaatgcaga aatagccaga ggaattgaag ccgcactact gcgagaaaca aacgcaaagg
    14401 taagtacttg ccagacggtc ttgtaatagt taacggggtt tttacctatt aacagcaaaa
    14461 tgcccgagtg aacaataaac atcacaaaaa cggccagata acaggcgacg atgaacccga
    14521 gcaaattggt aaactgtgaa agctgatacg ttgagaagac tttcgccatt agtgccatta
    14581 ccccataagg cgtcagcgca atgacaatac ggaccatttt cattacccat atttgaatcg
    14641 cgttgatccc cgcgctcaat ttttcgccct ctgctggtgc atctttcttt acttttaaca
    14701 gcgcaatacc agcaagaatt gtaaagatga cgatgccaat aaccgaaaca cttcttgccc
    14761 cggtcagatc aaggaagagg ttggttggga tcaatgacgt aatgagtcgc ggtaaagata
    14821 ccgcagctgt tttcgagacg tcatcgctgg ttaaagagga ttgcatatga ccaaaggcgc
    14881 tggcatcaag gccaattgca ctggcaatag ccaggccaac taaacctgca atcatgacta
    14941 aacacaacat acaggcaaca atagttaaag acatcttgcc aataccagcg ctattttcga
    15001 gcttattaat cgctgaaaga atcgaaatga atatgagtgg aacggcaaca agttttaata
    15061 aattaacata accgttacca gcaatattat accattcgat aacggtttca ctgcttgtct
    15121 caggaatgaa atgcagggag ataccaatga tagcccccag gaaaatcgcg ataaaaacac
    15181 ttttggaaag ggattcgctc ttttgatccg ttttgaaaat cccccatgag cagatcaaaa
    15241 aaagcacaat atatgtgaaa gttgtaaccg ttttttaagc ctcttgcctc attcagcacc
    15301 tgaaatgccg gaaagagttt gttgtaaaaa atctgcgcca taagacgcag ggacgaggcg
    15361 attatagaga aattttttct cattttcata tcaaatgtag ggggtttgga atttgaataa
    15421 gctcttatta attgaattcg ttaattgttc tctttttcag atgaacaaat attagtcatc
    15481 tgattgttta ttgtcactat cccatgcatg aggcgcaaaa agatagcctt tgttacgcac
    15541 agttttaatg cgataaggtt ctgcggcgtt atcgagcagt ttttttctta accgcgaaat
    15601 agccacgtcc acgctacgat ccagtccgtc ataactgacg ccgcgtaaat ttttcagcaa
    15661 tgcatcgcgg tccatgattt gcccggcatg cgtagctagt tcccacaata attcgaaatc
    15721 agctgtcgag agcgagattt cagtgttagc cagggtgact acgcggttga tggggtcgat
    15781 agttaacgtg ccgaaatgca gggctttgta gggagtaaga gacgtttcct gaagaccttt
    15841 ggtcagtgtg gcttgctcat tctgacgcaa atgcaaacgt aaacgcgcta gcaaaacagc
    15901 agggggcgtc gttttgagaa tatagtcgca ggcacccatt tccagtgcca ggatgtggtt
    15961 catatcgcta tcgagagagg ttagaagaac aatcggtcca gaccactttg cgcgtaaatc
    16021 acgacaaatg gtcatgccgt ccttgcctgg tagcatgatg tcgagtaaca ccaaatccgg
    16081 attttctcgc aaaatggttt cttcggcctg gtcgccgcgc ggttctacgg taacctgcat
    16141 atcatgtttt gccaggtacg cggcaatcag tgaaccgact tccgcatcat cttccacaaa
    16201 tacgatagtg ttcataacat tcacggtaga tataaaaacg tcacaataca ccgcgctgtt
    16261 tttacttact atcaatcgtt tctaaacaag ttttcatcag gtattctact gatgacttgt
    16321 tatggaagtg ttaaggtaaa aagatggggc tggtaatcaa agccgcgctc ggtgcgctgg
    16381 tagtgctgtt gattggtgtt ttagcaaaaa cgaaaaatta ttatatcgcc gggctgattc
    16441 cacttttccc gacctttgcg cttatcgcgc attatattgt tgccagcgaa cgcggcattg
    16501 aagccttacg cgcaaccatc atttttagta tgtggtcgat tattccctat tttgtttacc
    16561 tggtgtcgct gtggtatttc accggaatga tgcgcttgcc caccgctttt gttggttctg
    16621 ttgcgtgttg ggggataagc gcatgggtgt tgattatatg ctggataaag ctgcattaac
    16681 gcagatgacg accgccatca agtgggaaac tgcgtccggt gacaaagcaa ctggtaagta
    16741 agtaatcgac caggtcgatc acttctttct cgccaggcgc ggttttcatc agtgatttat
    16801 tcagcgcctg ttgtcgatat tcggcatcat catgttcatt aaacaggatc agcgatggcg
    16861 caatagaatt cactttcact tccggtgcca gcttgcgggc aaacgagcgg gtcatattat
    16921 ccagtgccgc tttgcttgca gcatacgcaa catgtttgtc gctaccgcgc tccaccacat
    16981 aatcggtaaa gtgaatgata tcgctggcgg cgtgtccgtg cccacgcagt aatctttcca
    17041 gcgcatggtt gagcaggtat ggggtattaa cgtggatctg catcatgcaa gccagtacgt
    17101 cggccagtgg cgcacccggt ttttccgcaa tccacgcact ggcgttatgc aaaatagcac
    17161 gcagaccatg ggtggttttt agtacttcat cggcaaacgc catcacaccg tcgttggtcg
    17221 aaaaatcagc ctgaatacac tgcgcacctg cattaatcag tccatcaatg gctggatagt
    17281 gtgtccgata gctgacaatc accggttgct tttgattaat gaaatgccat gcgagggcga
    17341 ggccgatgcg acgacctccg ccagtaatta atattggcaa gggctgggtt ttacccatcg
    17401 ttatctcctt tgctatccaa cgatgggatg aacgttatcc caccagcatc caggtcgccg
    17461 gaactgaggc aattaacagc atgccaatca gcaccatttc ctgacggttc agtacattat
    17521 catgtgtatg cgttttgcgc gcatacagga aaaccagcag ccccggtgca tacagaacaa
    17581 cggataacag caagtgcatt ggaccagagg cgtacaataa ccataagcca taaatgcagg
    17641 caccgacacc taccgcttta tgcagtggac gggttgcgat tttcagcaag aacgcgccga
    17701 ccaggaaata gggcaccaga atcatttctg aggcgatggt cagcaacgtg ttgtaatcgg
    17761 aaccggtcag ccagatgagt accagacaaa tttgcacgca gatgttggtc aaccacagtg
    17821 aggcggatgg cgcagcttgt gcgttctggc gcgcaaaaat gcgtgggaat gctttatgag
    17881 tggcagccag gaacggaact tctgccgcca tgatggtcca gcttaagtac gcgccgcaaa
    17941 cggaaacgat caaaccggca gcgatgatga tttcgcccca tggtcccatc atttccacca
    18001 tcagaccggc cattgacggg ttacgaattt cagccagttc aggacgtgcc accacaccca
    18061 gcgaaagcag cgttaccagc aagtaaacgc ccagagcgga gagaaccgcc agcagtgtcg
    18121 ctttgccaac atcacgttta ttacgcgcac gcgcagaaac aaccacggca ccttccacac
    18181 caatgaaaac ccacagggtg atcagcatgg tgtttttcac ctgttcccaa acaggtacgc
    18241 caagtgcaag tccggtgaag tcgagcttga aggtatccag tttgaacatc atcatcgcca
    18301 gcacaacaaa cagacccagc ggcaacaatt ttgccagtgt cgccaccagg ttaatgctgg
    18361 cagcggtttg caccccgcgc aggatcaaaa agtgaacaat ccacaataaa gccgatgcac
    18421 cgactatcga ttgccaggta ttaccatcgc caaacaggcg caattccggc gtgtccgtaa
    18481 aaaagcttaa cgcggaaaaa acgatcacca gataggagac gttggcgatg accgcgcaca
    18541 gccagtatcc ccatgcggaa caaaagccga ttagctcgcc aaacccttcg cgggcatagg
    18601 taaagatacc gccatcaagt tcgggacgaa tgcgcgtgag gatcagcatg gcaaaggcca
    18661 gcaataaaat gccagcgcca gtaatacccc agccgatgag cagtgctgcc gggctggcaa
    18721 ctgccgccat attttgcggc agactgaaaa cacccgcgcc cagcattgag cttaatacca
    18781 gcgcggtgag tgcgctcagt cccagtttct tttccatcga tatccagtga ttaaaacgtg
    18841 ataagaagaa taaatattag ggcaaaacgc ataaaaatgg agtttgccac tgaagcgcgg
    18901 gattttacga agagatgtgt ctgcatgcaa tggtcgcggg gaaatttgtg actaaaaatg
    18961 cgaaaggcgg catcaagccg ccttatagga gtaactgaat agttatttat acagatctgc
    19021 gctgacggtc aggttattac cacgtttctg ccactggcgg gtgatgtggt aatacttagc
    19081 acctttcttc gcggcacgtt tcgcaacctg ataggagact tcggtcatgt tgccgtagtt
    19141 gccagagaat ttgatgctgt cgaacgggac catcatcgct gctgtcgctt tgttcagttc
    19201 ttcgacttta gtgccatccg ggagcgtgac ggtgtaacgc ccgccttttg atgactgggt
    19261 ttcaaagaag cgaccgactt cagaacttgg tgatgcggta gtcgcaacac ccgggatctc
    19321 aactttcttc gcggcttcgc caccggcagc cagagctgca cgtcctgctt cggaatctgc
    19381 cgggatcaca tccgggctct ggacgatacg tttcttagca tcttttttat agatgaatgc
    19441 agtaatacgc tggttgccgc cctggttggc atcgatttga cgaacgatgt agaaagagta
    19501 ggcacctttc gcttttgccg ctttggtgat ggcatcattg acttctggct ggctacgata
    19561 gaagccctgg acggtgaccg tgtcaaacgg ttcaatcaga acagcctgat ctttcggcag
    19621 ttcgacaaca ccgttaatta cgcgattact tgtttcttct gctttttcag catcggcttt
    19681 atagaggtca gcgaccacac gccagttacc gctgttacca aaatcagaag tgtcgacaac
    19741 ataaaaagag gcggcacctt ctttatctgc gcgacgagaa acggctttca ccgcttcgcc
    19801 aatagcatta aaacgaccgg taaccactac acggtcaaaa ggtttaaccg ctgccgcttg
    19861 ctccggtgtc agttctgttg ctgcgttaac ggagaatgcc gtagcagaaa gcagtgccga
    19921 cgccaggagg gtgttcttaa gcttcataaa aataatcctt cgccttgcgc aaaccaggta
    19981 ctggtattgt tattaacgag aaacgtggct gattattgca tttaaacggt gtaactgtct
    20041 gcgtcatttt tcatatcaca ttccttaaac caattttaat cctgctcaaa tgaccgtcta
    20101 tgcttaaaaa acagccgtat cagcatcatt actactgaag caactgaatt gtataagtta
    20161 atttaatgtt aagtagtgat tcgtgccggg gcgatgtctc gttttacccg accgtcgaag
    20221 acaattatca gtctttatcc ggcgttctaa ggtgtttatc ccactatcac ggctgaatcg
    20281 ttaatatttt gcgagttcac gccgaaatac tgatttttgg cgctagatca caggcataat
    20341 tttcagtacg ttatagggcg tttgttacta atttatttta acggagtaac atttagctcg
    20401 tacatgagca gcttgtgtgg ctcctgacac aggcaaacca tcatcaataa aaccgatgga
    20461 agggaatatc atgcgaattg gcataccaag agaacggtta accaatgaaa cccgtgttgc
    20521 agcaacgcca aaaacagtgg aacagctgct gaaactgggt tttaccgtcg cggtagagag
    20581 cggcgcgggt caactggcaa gttttgacga taaagcgttt gtgcaagcgg gcgctgaaat
    20641 tgtagaaggg aatagcgtct ggcagtcaga gatcattctg aaggtcaatg cgccgttaga
    20701 tgatgaaatt gcgttactga atcccgggac aacgctggtg agttttatct ggcctgcgca
    20761 gaatccggaa ttaatgcaaa aacttgcgga acgtaacgtg accgtgatgg cgatggactc
    20821 tgtgccgcgt atctcacgcg cacaatcgct ggacgcacta agctcgatgg cgaatatcgc
    20881 cggttatcgc gccattgttg aagcggcaca tgaatttggg cgcttcttta ccgggcaaat
    20941 cactgcggcc gggaaagtgc caccggcaaa agtgatggtg attggtgccg gtgttgcagg
    21001 tttggccgcc attggcgcag caaacagtct cggcgcgatt gtgcgtgcat tcgacacccg
    21061 cccggaagtg aaagaacaag ttcaaagtat gggcgcggaa ttcctcgagc tggattttaa
    21121 agaggaagcg ggcagcggcg atggctatgc caaagtgatg tcggacgcgt tcatcaaagc
    21181 ggaaatggaa ctctttgccg cccaggcaaa agaggtcgat atcattgtca ccaccgcgct
    21241 tattccaggc aaaccagcgc cgaagctaat tacccgtgaa atggttgact ccatgaaggc
    21301 gggcagtgtg attgtcgacc tggcagccca aaacggtggc aactgtgaat acatcgtgcc
    21361 gggtgaaatc ttcagtacgg aaaatggtgt caaagtgatt ggttataccg atcttccggg
    21421 ccgtctgccg acgcaatcct cacagcttta cggtactaac ctcgttaatc tgctgaaact
    21481 gttgtgcaaa gagaaagacg gcaatatcac tgttgatttt gatgatgtgg tgattcgcgg
    21541 cgtgaccgtg atccgtgcgg gcgaaattac ctggccggca ccgccgattc aggtatcagc
    21601 tcagccgcag gcggcacaaa aagcggcacc ggaagtgaaa actgaggaaa aatgtgcctg
    21661 ctcaccgtgg cgtaaatacg cgttgatggc gctggcaatc atcctgttcg gttggctgtc
    21721 aagcgttgcg ccgaaagagt ttcttgggca cttcaccgtt ttcgcgctgg cctgcgttgt
    21781 cggttattac gtggtgtgga atgtatcgca cgcgctgcat acaccgttga tgtcggttac
    21841 caacgcgatt tcagggatta ttgttgtcgg agcactgttg cagattggcc agggaggctg
    21901 ggttagcttc cttagtttta tcgcggtgct tatagccagc attaatattt tcggtggctt
    21961 caccgtgact cagcgcatgc tgaaaatgtt ccgcaaaaat taaggggtaa catatgtctg
    22021 gaggattagt tacagctgca tacattgttg ccgcgatcct gtttatcttc agtctggccg
    22081 gtctttcgaa acatgaaacg tctcgccagg gtaacaactt cggtatcgcc gggatggcga
    22141 ttgcgttaat cgcaaccatt tttggaccgg atacgggtaa tgttggctgg atcttgctgg
    22201 cgatggtcat tggtggggca attggtatcc ggctggcgaa gaaagttgaa atgaccgaaa
    22261 tgccagaact ggtggcgatc ctgcacagct tcgtgggtct ggcggcagtg ctggttggct
    22321 ttaacagtta tctgcatcat gacgcgggaa tggcaccgat tctggtcaat attcacctga
    22381 cggaagtgtt cctcggtatc ttcatcgggg cggtaacgtt cacgggctcg gtggtggcgt
    22441 tcggcaaact gtgtggcaag atttcgtcta aaccgttgat gctgccaaac cgtcacaaaa
    22501 tgaacctggc ggctctggtc gtttccttct tgctgctgat tgtatttgtt cgcacggaca
    22561 gcgtcggcct gcaagtgctg gcattgctga taatgaccgc cattgcgctg gtattcggct
    22621 ggcatttagt cgcctccatc ggtggtgcag atatgccggt ggtggtgtcg atgctgaact
    22681 cgtactccgg ctgggcggct gcggctgcgg gctttatgct cagcaacgac ctgctgattg
    22741 tgaccggtgc gctggtcggt tcttcggggg ctatcctttc ttacattatg tgtaaggcga
    22801 tgaaccgttc ctttatcagc gttattgcgg gtggtttcgg caccgacggc tcttctactg
    22861 gcgatgatca ggaagtgggt gagcaccgcg aaatcaccgc agaagagaca gcggaactgc
    22921 tgaaaaactc ccattcagtg atcattactc cggggtacgg catggcagtc gcgcaggcgc
    22981 aatatcctgt cgctgaaatt accgagaaac tccgcgctcg tggtattaat gtgcgtttcg
    23041 gtatccaccc ggttgcgggg cgtttgcctg gacatatgaa cgtattgctg gctgaagcaa
    23101 aagtaccgta tgacatcgtg ctggaaatgg acgagatcaa cgatgacttt gctgataccg
    23161 ataccgtact ggtgattggt gctaacgata cggttaaccc ggcggcgcag gatgatccga
    23221 agagtccgat tgctggtatg cctgtgctgg aagtgtggaa agcgcagaac gtgattgtct
    23281 ttaaacgttc gatgaacact ggctatgctg gtgtgcaaaa cccgctgttc ttcaaggaaa
    23341 acacccacat gctgtttggt gacgccaaag ccagcgtgga tgcaatcctg aaagctctgt
    23401 aaccctgacg gcctctgcgg aggccgtcac tctttattga gatcgcttaa cagaacggcg
    23461 atgctttgac ctcccgctgt ttgttcaagc gcaattttga caataattgt caacggcacg
    23521 gaaagcagca tacccaccgg tcctaacaac catccccaaa aaatcaacga caaaaatacc
    23581 accaatgtcg aaagccccag cccacgtccc atgatgcgcg gctccagaat attgccgaag
    23641 accagattaa tcagcagata tcccgccagc accagcaacg cttcgtagaa gccattaaac
    23701 accagtacct gagcgatagg ggggattgcc gcgagaactg aaccaatatt agggatgtaa
    23761 ttaagcgcaa aggccagcaa tccccagaca aaagcgaagc gaacatcgag tgcggcgagc
    23821 atcgcccagg cgaccaggcc ggtgatgatg ctgatggctg ttttcagcac cagataatga
    23881 gaaacactgt caatcgcacg ttgaatcgcc gccatccctt caaccggacg cgccatcatt
    23941 tgctgaaatt ttccgggcaa ttgtggcact tcgagcagca taaacagcac cgtcagcagc
    24001 aataaaaata ttgatgacat ggcattagat aactgcgtca ataagttggt gagcaacgtc
    24061 atcgccgcgt tcggatcaat gtaatgcgcc agttggtcaa ctgagacgtc aatccctacg
    24121 cgttgcaaca acggctcaag agcttgcagc ggcgtcataa tagagttgcg atattgcggt
    24181 aacgtccacg tcaactcgtt gagcgcggaa cccagataag ctaatagcaa caccatcgcc
    24241 atcacgatga ttgtcatcaa aatcgacact gccagtacac gtggcacacg ccagcggacc
    24301 atatgttgca ccagcgggtt aagaataaca gcaataaata atgcgagaat aaacggcacg
    24361 atgatctcgg cggcaaaacg gataccgcag agaataatga ccagcattcc caacataatg
    24421 acgattttta ggccattgag cgtgatgatc ggctttgcca tgcttgtccc cgtttttctt
    24481 cctttgctaa taataatgtc tttttcgctc cgtcatcata acgaaaatca aaccgctcag
    24541 gtaaagaagt gaaaatattt tgagttaatt cttaagctat gatacaaatc aggcgtgttc
    24601 aactaccgag gacaattatc atctgcgatg acgagaagca acactgcgga taattgtaat
    24661 attatggaca atttgttcag gacgttattt tctacattta cccatcttcg tacctcatcc
    24721 actatcttgc tcgtaggtga gcagcactgg cgcaacgcgc tttagtcctt tcttctgcct
    24781 aagcttgccc tatgcgcagg gcttgaccac tttactttgg tttcgctgaa ttaagcgaaa
    24841 attaaaatag ttctcttgca ggagaaggac aatgtatatt tattggattt tattaggtct
    24901 ggctattgct acagaaatta ccggtacgct gtcaatgaaa tgggcgagcg tcagtgaggg
    24961 aaatggcggc tttattttaa tgctggtgat gatttctctg tcgtatatat ttctctcttt
    25021 cgccgttaaa aaaatcgcct taggcgtagc ttatgcgctg tgggaaggta tcggtatttt
    25081 atttattacc ttgtttagcg ttttgttatt cgacgaaagt ttatcgctga tgaaaattgc
    25141 cgggttaacc accctggtcg ccgggatagt gttgatcaaa tcaggtaccc gtaaagcgcg
    25201 taaacctgaa ctggaggtga accatggcgc agtttgaatg ggttcacgcc gcctggctgg
    25261 cattggcaat cgtgctggaa atcgttgcta acgtcttttt gaaattttct gacggctttc
    25321 gtcgcaaaat atttggcttg ctctccctgg cggcggtgct ggcggccttt agtgcgcttt
    25381 ctcaggccgt taaagggatc gacttgtctg tcgtttatgc attgtggggc gggtttggta
    25441 ttgccgccac gttagccgca ggttggatct tgtttggtca acggttaaat cgtaaaggct
    25501 ggattggcct ggtcttgctg ttggctggaa tgatcatggt gaaacttgcc tgatgaagac
    25561 gctgcccgcg ctgtcgggca gcgtttgaac attatttttg cgacagttga tccagcttgt
    25621 cgcgaaaacc ggtaacagaa atggcccggt tatcggcgcg ccagcgatct ttcgcggcag
    25681 gagccgaact ttgcacccca attaattgcc agccgtcatc ggtatgcaac atcagaggcg
    25741 aaccgctgtc acccggcaag gtatcgcact gatgtgacat caccgacgtt tgcgcccagc
    25801 cagtcacttc acagttttga tgactgtata acgtatcgag atgatcttca gggtatcccg
    25861 cctgagtcac tttacgacca gccgatttta atgcggcagt aagcgcggct ttatctccct
    25921 caaataacgg caacggtgta atgccagaag ggggattacg tagcacaatc aatccgaagt
    25981 cccacggtgc agctgcggga ggaacaatcc aaccatcccc atctgctttt aaccgctttc
    26041 ccagtgtcgg atcaacgcgg ccttctatgt cgtggatctc atagcgccaa agacctttat
    26101 ttgacacaaa acgcagcgct accgccttat ccgctttacc cttaggcggc gtcaataaac
    26161 agtgtcctgc cgttaatgcc agattgggtg caatcagcgt cgccgtacat aaattgccgc
    26221 tggccgtttc cagttgccca accgcatccc acggtgattg ggtcgtgtca ttcactggca
    26281 cacgatcatc atgaccaaaa aacagggtgc tgacctcatc gtttgccgat ttggcaacgt
    26341 ctggtttatc tgcaaacaca aaagcagacg tcaaactaat tgcacccaac actacagcaa
    26401 tggttgtacg catatcacac tctggtgggt aattatgatt attaaaagca aaccctcata
    26461 aatactatag acgggacggg atgaaagtgg gagtaaaatc agataactac agtcaggaaa
    26521 gataaaaacg ggcggcaatc agtgcgcata aaataaggat gataagaatg aactcaaagc
    26581 gataacggcc caccatacta ccctccgcta aaggcggtgc taaacgttac ccgcttagca
    26641 ccgtaatttt caggcgcgag ggggcgcgcc agcattactg ttgaaaactt acgctgcggg
    26701 ttgtgcagca ggttttgctg gttgctgatg gccgtgtttc ccggcgtgtt tcttcgctgc
    26761 ctgcgctttt tgttcagggg ctttctgttc agctttcgta tttttatgat gctttttagc
    26821 cgcctgcgct ttctgggcag gtgctgcttt atgctgtttt ttatgatgtg tagttttcgc
    26881 tggcgctgct ttggtggtcg tcgcagtcgg agcaggtgtg gtcgcagtct ctgcagcaaa
    26941 ggcggcagaa gacagaccca tagcagcggc aacaaccaga gctaatactt ttttcattgt
    27001 cataccctca atttgttttt tcatttaacc ccactgcggg gccgttgaaa taactatatc
    27061 cctgggaatg gcagacttcc gtgagtggtt ggtttcagcg tgtaacgata tgtacaaacg
    27121 ctgaataaat tacagcgttg ataataacgt attgtgactt aaggaaaatt tagctaccaa
    27181 taatagtagt cttgatcggg ttaactatta tactacttac aaagcggtag aataactgcg
    27241 catcagtaat aaatgacaca cagcaaaatg aatccgttta tttgggtact tataatcctg
    27301 atgacgctag atgcgctgcg ggaattggct ggcgcttcgt ctattttagg atggctatta
    27361 acgttggttt gagcaggcaa taagtccgga cgggtattta ccgcagtccg gacttatttt
    27421 tcaggcgtgc agacgacgat gcaaacgcgt cccgaccagc agagcaatga ccagcatcag
    27481 agcaataaat gcgccgacgc cgttccagcc atagttatgc cagaaaacac cacccagcgt
    27541 cccggcaata ctcgacccca gatagtaact gaacagatac agcgaggagg cctggccttt
    27601 agcgcgtttt gcgcgggggc cgatccagct gctggctact gagtgggctg cgaagaatcc
    27661 ggctgagaag agtaacattc cggcaaagat cagccacagc gagctaaata aggtcatcag
    27721 taaaccaaac ggcataactc ccgtcgaaaa caacatcacc ggaccgcgcc catagcgggt
    27781 ggtcatggtt ccggctttgg gtgagctcca tgtaccggtc aaataagcca gcgataataa
    27841 gccaaccacg gcctgactga catgctaggg ggaaagcatc aaccgatagc cgatgtaatt
    27901 aaacagcgtg acgaacgacc ccatcagcaa aaagccttct gcgaacaata acggtaatcc
    27961 ccggtcacgc cagtgcagac gaaagttgat aaataacgtc ttagggcgca gcgaagtcgg
    28021 gcaaaaatgg cgtgattcag ggaggatttt ccagaacatc aacgccgagg ccagcgcgaa
    28081 acaaccgatt gccgccagag caattcgcca gttgaaaaag tccgtgaaga caccactaat
    28141 taagcgtccg ctcatgccgc caattgagtt gccgctgata tacaacccca tcgaaaaggc
    28201 cacgaaactg gggtggattt cctcgctaag ataagtcatg ccaacagctg ccacgccact
    28261 taacgaaagc ccaatcaagg cgcgcataat caaaatgccg tgccagctgg tcatcattgt
    28321 cgaaagtaac gtacaaatgg aggccaacag tagcgccgtg accatcactg gtttgcgacc
    28381 aatggcatcg gatagcgggc cagtaaatag caaaccgata gccaacatcg ccgtggaaat
    28441 ggacagtgaa atactactgt tcgcgggcgt aaagccaaac tcctgcgaaa gcaccggaag
    28501 gataggctgc acacaataga gaagtgcaaa ggttgccagt ccggcagaga acagcgccag
    28561 ggtgacgcgc ataaattgcg gcgtaccgcg tttaataaat tgatttggct gagaaatgct
    28621 ttgcttgtca gtgtcgcttg ccggagcgcc atcaacagtt gtagtacggc tcacttgaaa
    28681 tccttgctaa atatgcctgt ggatcaggct tatacatagg gtaggaaaat cgaattgttc
    28741 tgtctaatat attaataatc tcaaataaga tgttttaaat atgaatattg aacttcgtca
    28801 tctgcgttac tttgttgctg ttgcggaaga gctgcatttt gggcgcgcgg ctgcccgcct
    28861 gaatatttcg caaccgccgc taagtcagca gattcaggca ctggagcaac aaatcggtgc
    28921 ccgactgctg gcacgaacta atcgcagtgt attgctgacg gcagcaggaa aacagtttct
    28981 tgcagatagt cggcaaatcc tgtctatggt ggatgacgct gccgctcgcg ctgaaagact
    29041 gcatcagggt gaagcggggg agttgcgtat tggttttact tcgtccgctc cttttattcg
    29101 ggcggtgtcc gatacgttat cgctgtttcg ccgtgattat cctgatgtcc atttacaaac
    29161 ccgcgaaatg aacactcgcg agcaaatcgc tccgctcatt gaaggaacgc tggatatggg
    29221 attgctgcgt aacacagcgt taccggagac gcttgaacac gcagtcatcg tccatgaacc
    29281 gcttatggcg atgatcccgt acgatcatcc cctggcaaat aatccgagtg taacactggc
    29341 tgaactggct gaactggcga aagaaccctt tgtctttttt gatccgcacg tcgggactgg
    29401 gctgtatgac gatattctcg gactgatgcg acgttaccat ttgacgcccg tcatcactca
    29461 ggaggtgggc gaggcaatga ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat
    29521 tttgcctgcg tcatttaaac gtgttcagct caacgaaatg cgctgggtgc cgattgctga
    29581 agaggatgcg gtttctgaaa tgtggttggt ctggccgaaa catcatgaac aaagtccagc
    29641 tgcgcgtaac tttcgtattc atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa
    29701 aagcccgaaa aatgtgctgt taatcacatg cctaagtaaa aatttgacga cacgtattga
    29761 agtgcttcac catagcctac agattatttc ggagcgcgaa aatataggga gtatgcggtg
    29821 gttgctgaaa accagcctgg gcacattgat caaataaagc agaccaacgc gggcgcggtt
    29881 tatcgcctga ttgatcagct tggtccagtc tcgcgtatcg atctttcccg tctggcgcag
    29941 ctggctcctg ccagtatcac taaaattgtc cgtgagatgc tcgaagcaca cctggtgcga
    30001 gagctggaaa tcaaagaagc ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa
    30061 actgaagcct ggcactatct ttctctgcgc attagtcgcg gggagatttt ccttgctctg
    30121 cgcgatctga gcagcaaact ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac
    30181 ttgccattgc tggatcgtat catttcccat atcgatcagt tttttatccg ccaccagaaa
    30241 aaacttgagc gtctaacttc gattgccata accctgccgg gaattattga tacggaaaat
    30301 ggtattgtac atcgcatgcc gttctacgag gatgtaaaag agatgccgct cggcgaggcg
    30361 ctggagcagc ataccggcgt accggtttat attcagcatg atatcagcgc atggacgatg
    30421 gcagaggcct tgtttggtgc ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat
    30481 cacaacgtgg gggtgggcgt cattaccgat ggtcatctgc tacacgcagg cagcagtagt
    30541 ctcgtggaaa taggccacac acaggtcgac ccatatggta aacgctgtta ttgcgggaac
    30601 cacggctgcc tcgaaaccat cgccagtgtg gacagtattc ttgagctggc acagctgcgt
    30661 ctcaatcaat ccatgagctc gatgttacat gggcaaccgt taaccgtgga ctcattgtgt
    30721 caggcggcat tgcgcggcga tctactggca aaagacatca ttatcggggt gggcgcgcat
    30781 gtagggcgca ttcttgccat catggtgaat ttatttaacc cacaaaaaat actgattggc
    30841 tcaccgttaa gtaaagcggc agatatcctc ttcccggtca tctcagacag catccgtcag
    30901 caggcccttc ctgcgtatag tcagcacatt agcgttgaga gtactcagtt ttctaaccag
    30961 ggcacgatgg caggcgctgc actggtaaaa gacgcgatgt ataacggttc tttgttgatt
    31021 cgtctgttgc agggttaaca ttttttaact gttctaccaa aatttgcgct atctcaattt
    31081 gggccaggaa agcataactt agactttcaa ggttaattat tttcctagtt tatatttgtg
    31141 aagcataacg gtggagttag tgatgctgaa gcgtttcttt attaccggta cagacacttc
    31201 tgtagggaaa acggtggttt cccgcgcatt gctacaagcg ttagcctccc agggaaaaag
    31261 ggttgcggga tataaacccg tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa
    31321 taaagatgcc ttggtgttgc agagtgtttc aaccatcgaa ctgccttatg aagcagttaa
    31381 tcctatcgcg ttaagcgaag aagaaagtag cgtggcgcac agttgcccaa tcaattacac
    31441 cctcatttca aacggcctgg caaacctgac cgataaagtc gatcatgtcg tggtagaagg
    31501 gactggcggc tggcgcagtc tgatgaatga tttgcgtcca ctctctgaat gggtagtgca
    31561 ggaacaactg ccggtgttga tggttgtcgg tattcaggaa ggttgcatta accatgcact
    31621 gctaacagct caggcgatcg ccaacgacgg gctgccgctc attggctggg tggctaaccg
    31681 aatcaaccca ggactggcgc attatgcgga aatcattgat gtgctgggta aaaaacttcc
    31741 ggcaccgctc attggtgaac tgccttatct gccgcgcgcc gaacagcgtg aactggggca
    31801 atacatccgc ttagctatgc tgcgcagtgt gctggcggta gatagagtca cggtgtaacg
    31861 tccgcgaaat taccgacgca attacgcagg caatcaataa accggggagt agctgatact
    31921 ccccggtcat ttcacatatc atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca
    31981 acagtgtcgc catcccggtc aatccgagta aaagcgtaat ttcttcgcca tcagggaacc
    32041 ataatcccag gctacgacca tacaacatgc caatggcaag accgataaat agcgtcggtg
    32101 taaagacacc accgggtgcg ccggaaccgc tactcgccag cacggcacac agtttacaaa
    32161 ggaagatccc ggcaatgatc attaacagtg gtggggcggt taaaaaggat tgtacggtgc
    32221 tatagccgtt gccccacact gcaggtgtga acagggaaag cagacccacg atcaacccgc
    32281 ctagtgccag ttgccagggc ggcgcaagtt tgagactcac aaatccacga tgacaggcgt
    32341 tcattaatgt taacaacagt ggtccgcaca gacctgccag cacacctgta ctgataatca
    32401 gcgcatagtc acgagcctga accgtcactg agagttgtac gttgtagagt aacgcgtcgc
    32461 tatgattaat cagattgcta accagccatg ccacgatggc ggaaataatc accgggccga
    32521 gagaggccaa catcatagtg ccaaacagca cttcggctat aaataaactg ccagcaagcg
    32581 gggcacgata ggccgcagcc attcccgccg cggccccaca ggcgatccat aatttccatt
    32641 cctggcgtgg cgtaaaacgt tgggcaaaac aggaggcggc aagggcagct aaaagaatca
    32701 tcgcaccttc gcgaccaatt gcactgccgc tggttactac cagcagcgag gcaagcgatt
    32761 taaccaggct tgctgcgtaa tcgaactgtc catcggtttg caacgcttcc atgtaatcgg
    32821 tcggcgcatg agggcgttgt tgggtaaatt tctgccagcc catcagcaac aaacccgccg
    32881 ccagtccgcc gagcgccgga gttagcagcc gtcgccagga ggaaaggttt gtcgctgcat
    32941 tgaccagact gccggagtca ttattgagga acaaccactc cagtagcagc atcgcatgac
    33001 gaaacccggc aacggcaaag acagcgagaa taccgacgac tgttgcgata agcagacggc
    33061 ggaacatagt gcgcagatcc ggatagatgt gtagatgatg catgagcaga aatgggcaat
    33121 gaaacacggg cgactatttt gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta
    33181 agagataaaa aaacgtgaca ttgtaacgta ttattgccga atcgcgcgta atcgtcacca
    33241 tccggcaata ttacggtgat cctatcgaaa gagtggttta accgcgacag gaatcaacag
    33301 ttgtgattgc cattgcgcca gcgttaagcg agccagttca cccagtgcac ggtagaaagg
    33361 gtgttctgct ttttcgataa aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca
    33421 tgccagcaat tcttcgcatt ctgtctggcg accattctcc gccaaccacg cagccatcaa
    33481 cagcagtgaa ccaaaatgat cttccggttc gttttgcttc atttcaaact gaatgccttt
    33541 ctcgcgcatc cactgacgaa gtgccaatgt tgaatcgcca aacagcacag attcgcgatc
    33601 cagccagacc gacccccacg gcggagacgg cagtgcccac gggccgacaa acaaacgctg
    33661 ccaggcctgg gcgtgagtct cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa
    33721 tgacgcttct ggtagaggcc actgagtttc ccagccgtca ctggtgagta ccgcaaccag
    33781 aggtgcggct tccgcactct ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc
    33841 gacagaaaaa ttatcttgct gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg
    33901 ttgccccgca ccaatttgtt aacctgcaat agccattcct actgtcatgt gcaggccata
    33961 aaagagcaca cgcccgatca tctcaccaac aagtatgagg attagcccca gaacaagtcc
    34021 ggcaacgtga ggttcacggc gacgaatgag cggacaaagc cagcatccaa gacctgcgca
    34081 taacaatacc acacgccaga cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg
    34141 ttgcacggat gagtgaatag acgccagaga caaaccttgc agcacaatca cctcggcaca
    34201 agcgatcagc gccagaacgc taatgatggc aaatggtgtg gtattaaatg taacgcgggc
    34261 tgcccgcaga attgcggcag caagtattgg accgctcaac aataccgtca ggaagaaagc
    34321 cagcgtagtg taaccgttat gccaggttgg cacggtgtcg atttgataca cgcaggtcat
    34381 catccagacg aaaatgacgc cgagcgccat gctgacaagt aaccagagtt tccccaacgc
    34441 ttgcggcatt ttaccgatga ccgccaccag ccaccatagt ccgccaacgg caaaaaatat
    34501 ggaaccggca gcaatctcgt tactcagacc agaagctccg attcgattca gcgagttaaa
    34561 cgctcgcagt ggcgatccca ggtgcatgac agaggcaata aacccgatgc ccatcaacag
    34621 ccagaggaaa aacatgccac gaacaatgtg ctgacggtct gcgtcatttt tggcggcgaa
    34681 ccagccaatt ccgctaacaa tcagcgcgcc tactacgcat tgacccaata cggtaaagat
    34741 aaccagtggc cattcatgcc atccatttcc cattttacac ttcctccgga ttagccagat
    34801 aaccagtggt atcgccggtc gggcggctgt tggcgttagg tttgataacg atattgggtt
    34861 ttgtgaaatg cgcgcgcggc agtggcgcga cggcagccag agtgccgtgc ttctggcgca
    34921 gctcttcaat tggaccgaac tcaagtgcac gcagcgggca ggattcgacg catatgggtt
    34981 gtttcccctc ggcgacgcgc gaataacagc catcgcactt cgtcatgtgc cctttttcag
    35041 cattgtactg tggtgcgccg tacggacaag ccatgtggca gtagcggcag ccgatacaaa
    35101 catcttcatc aaccaccaca aagccatctt cacgcttatg cattgcgccg ctggggcaga
    35161 cttttgtaca tgcggggtca tcgcaatggt tacaggagat ggagagataa taagcgaaca
    35221 cgttctggtg ccagatgcca ttatcttcct gccagtcgcc gccagcatat tcataaatac
    35281 ggcggaaact gacttccggg ccaagatctt taaaatcttt gcacgccagt tcgcaggttt
    35341 tacagccagt acagcggctg gaatcgataa aaaatccata ttgtgtggtc atgggctact
    35401 ccttaaacct tttcgatctg gacaagattg ctgtgcgacg ggtttccctt tgccagcggt
    35461 gaagggcggt gagaggtcag aatattgata ctgccgccgt gatcgacccg gtcaccaaac
    35521 atatccgctt taagccacgc accttgcccg atggcggtaa cgccaggcag aatacgcgga
    35581 gtcacttttg cggcaatcag catttctcca ttattgttaa atacccgcac ggtatcgcca
    35641 tgacggatac cgcgtgcctg agcatcaatg gggttgatcc acacctcttg tgggcaggcc
    35701 tgctgtaaca catcaatatt gccgtagctg gagtgggtac gcgctttggt ttggcgactt
    35761 cactcagcat ccagtagatg ggtttgcgtt caaattttgc tgaggttgcg ggttgggcga
    35821 ggatcacata gcccatattc cctgcagatt catgagagat aaggtcttct tgctctgttg
    35881 gcatcaggtc aggcaacagg atatcgcaat acttagccga ggccgtcatg aagtggtcaa
    35941 tgccaacaat catctcgcac ttgctgtcat cctgaagcac ctcatgggtg tgattgatgt
    36001 cgccatgttg attgatcaat gtgttactgg cgtagcacca taaaaacttg atggggacat
    36061 ccagtttttc ttttccacga acaccatcac gggtcgcggt cattttcgta ccatggtcga
    36121 tggcatctgt ccatgtaaaa acggaaatct gcgttttaac aggattctcg agcatcggga
    36181 accattctac ccccagatcc cagctacctt cgcgtacacc tgagttgccg ccgtttatgc
    36241 cgacgttacc ggtaagaacg gaaagcatgg caatagcgcg ggacgtttgt tcgccgttgg
    36301 agtgtcgttg tggcccccaa ccctgacaaa tataagcagg ttttgctgaa ccgatctctc
    36361 gtgccaactg gataattttt tctgccggga tgctggtgat ttttgctgcc cattccggcg
    36421 ttttagctat gccgtcaggc ccttcgccca gaatataggc tttataatgc gcgttacgtg
    36481 gtgcgttggc gggcagcgtt ttttcatcgt aaccaacaca atatttgtcg agaaatggct
    36541 gatcgaccat gttttcagta atcagtaccc aggcaatcgc acaggccagt gcgccatcgg
    36601 tgccagggcg aatgggcagc cattcatctt cacgcccggc agctgtgtcg ttatagcgtg
    36661 gatcgatgac gatcatgcgt gcgtttgaac gttcgcgggc ttgctcgacg tagtaagtga
    36721 caccaccgcc gctcatccgc gtttctgccg ggttatttcc gaacataacg accagtttcg
    36781 tattggcgat atcatccggg ctgttgccat cattggcacc gaacatataa ctcattgcgg
    36841 cactgatctg tgcggtactg tagctgccat agcgactgag aaaacccccg caagagttca
    36901 tcagacggta cgggacgttc gagttggtga tatttccgcc atctacgcct gttccgtaca
    36961 ggacatgtac agcctcattg ccgtaatctt tcaggatccg ccgaagatta tcactgatgg
    37021 tatccagggc ttcgtcccaa cttatccgtt caaatttacc ttcaccgcgc tcgccgacgc
    37081 gcttcatggg atatttcaac ctgtcaggat gattcatccg tcggcggata gagcgcccgc
    37141 gtaaacatgc ccgaacctga tgattaccgt aaacgtcgtc acctgtcgta tcagactcca
    37201 cccagtacac ggtgtcatct ttcacatgca aacgtagcag acagcggctc ccgcagttaa
    37261 cggtgcagga actccagacc gttttctctt ctaccggagc ctctgccgcc cggaccattt
    37321 gggaaaatgg cagagtgaaa gcactgcttg ccagcgcgag actgccaagt gcggaggttt
    37381 tcatcagact tctatggctg atttcagcct tcatgagcgc ctctgtggta tggattttca
    37441 tcattactca cttattgctt ttcaaacaaa atgtcatgcc agaatttatg gttgtagtgg
    37501 gttatatttt ttcgatctcg accagattag tgtgctgcgg gtttcccttc gccagtggtg
    37561 aagggcgcag agtggttagc gtattcacac atccgccatg gtcgatttta tcgccagaca
    37621 tattgggctc gtgccaggct ccctggccca tagcgctaac tccagggaga atgcgtggtg
    37681 ttactttggc tggtagccga acttcgccac gatggttaaa cacccgcacc atatcgccgt
    37741 tggcaatccc acgtttctgt gcatctatag ggttgatcca cacctcctga cggcaggctg
    37801 ccttcaggac atcaatgttg ccgtaggtcg agtgagtacg ggatttgtaa tggaaaccaa
    37861 acagttgcag tgggaaggtt ctacgttcag gggagtccca gccttcaaag gttgaggcat
    37921 aaactggcag tgggcttatc acttcatctt tttccagttc ccaggtacgg gcaatttccg
    37981 ccagcttgct ggaataaatt tcaatcttac cggaaggcgt tttaagtgga tttgcctcgg
    38041 ggtcgtcacg aaatgctttg taggcgacaa aatggccatt gggatcttta cgcttataga
    38101 tacccatttt tttcagttcg tcgtaagacg gtaacgccgg atctttggca agcattttgg
    38161 cgtacagatg ttgtaaccat tgttcctgcg tgcgaccttc tgtgaacttt tgatagacgt
    38221 caggtccaag acgtttcgcg acttcactca ggatccagta aatcggttta cgttcgaatt
    38281 tttcgctggt gactggctgg aggaaaatga gatatcccat gttaccggcg tagtcgttag
    38341 gaataatatc ttcctgctca acggtcatca ggtctgccag cagagtgtcg gcatattttg
    38401 ccgatgaggt cataaagttt tcgatgacca caatcatttc gcatttcgat tcgtcctgca
    38461 gaatttcatg cgttttgttg atgtcagaat gctgattaac gagggtattt cccgcgtagt
    38521 tccagatgga ctccactgtc agacggatgg acggacttga agtagttgac tcaacgctac
    38581 ttaaggtcct gatatgactg acattaacca tgacgatact gcatatccat ccagttcttt
    38641 ctctgggtct ccctatctgt atcagcaagt ctacacctca agtcctgaca caaggctgac
    38701 acaaatcaag gaccttgcta cgccacagct agtccggaga accaacgggc gttacactgt
    38761 ccgcttcaga cttaaaggag ctaccagccc gtttctatca gtctcaaccc gcagcacaga
    38821 ccggaggacc gctacaatgc gtcagagaga gctcgcagcc acagctaagg cattcctact
    38881 ggacaatcct gaagtcacac tacaggagct tacagcgcac atacggtcca tggctgagca
    38941 gttcctgact gatgccagtg atgactactg gaatggcgta gaggtggcga cactggttga
    39001 cgagaagtcc aacctcaggg aacttgcagc gacaagagcg ttgagtgtgg accagcagag
    39061 gggcattaag ttagcgctgg aagtcctgac cgcagcacag cagagagtcg acactggtga
    39121 tacctctggt ctgattaagc tgattgatgg
//
LOCUS       DAJVPP010000029        37848 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVPP010000029 DAJVPP010000000
VERSION     DAJVPP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.029"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            12..53
                     /gene="hisL"
                     /locus_tag="OQM34_001466"
     CDS             12..53
                     /gene="hisL"
                     /locus_tag="OQM34_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1451967.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    29..152
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            199..1098
                     /gene="hisG"
                     /locus_tag="OQM34_001467"
     CDS             199..1098
                     /gene="hisG"
                     /locus_tag="OQM34_001467"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1451968.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1104..2408
                     /gene="hisD"
                     /locus_tag="OQM34_001468"
     CDS             1104..2408
                     /gene="hisD"
                     /locus_tag="OQM34_001468"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1451969.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2405..3475
                     /gene="hisC"
                     /locus_tag="OQM34_001469"
     CDS             2405..3475
                     /gene="hisC"
                     /locus_tag="OQM34_001469"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1451970.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3475..4542
                     /gene="hisB"
                     /locus_tag="OQM34_001470"
     CDS             3475..4542
                     /gene="hisB"
                     /locus_tag="OQM34_001470"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1451971.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4542..5132
                     /gene="hisH"
                     /locus_tag="OQM34_001471"
     CDS             4542..5132
                     /gene="hisH"
                     /locus_tag="OQM34_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1451972.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5132..5869
                     /gene="hisA"
                     /locus_tag="OQM34_001472"
     CDS             5132..5869
                     /gene="hisA"
                     /locus_tag="OQM34_001472"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1451973.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5851..6627
                     /gene="hisF"
                     /locus_tag="OQM34_001473"
     CDS             5851..6627
                     /gene="hisF"
                     /locus_tag="OQM34_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1451974.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6621..7232
                     /locus_tag="OQM34_001474"
     CDS             6621..7232
                     /locus_tag="OQM34_001474"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1451975.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7418..8395)
                     /gene="wzzB"
                     /locus_tag="OQM34_001475"
     CDS             complement(7418..8395)
                     /gene="wzzB"
                     /locus_tag="OQM34_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1451976.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8541..9661)
                     /gene="ugd"
                     /locus_tag="OQM34_001476"
                     /pseudo
     CDS             complement(8541..9661)
                     /gene="ugd"
                     /locus_tag="OQM34_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(9911..11317)
                     /gene="gndA"
                     /locus_tag="OQM34_001477"
     CDS             complement(9911..11317)
                     /gene="gndA"
                     /locus_tag="OQM34_001477"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1451977.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11426..11785)
                     /locus_tag="OQM34_001478"
     CDS             complement(11426..11785)
                     /locus_tag="OQM34_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451978.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRIYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(11782..12288)
                     /locus_tag="OQM34_001479"
     CDS             complement(11782..12288)
                     /locus_tag="OQM34_001479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451979.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12434..12997)
                     /locus_tag="OQM34_001480"
     CDS             complement(12434..12997)
                     /locus_tag="OQM34_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1451980.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13204..14142)
                     /locus_tag="OQM34_001481"
                     /pseudo
     CDS             complement(13204..14142)
                     /locus_tag="OQM34_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14205..14579)
                     /locus_tag="OQM34_001482"
     CDS             complement(14205..14579)
                     /locus_tag="OQM34_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCS1451981.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14583..15731)
                     /gene="wzy"
                     /locus_tag="OQM34_001483"
     CDS             complement(14583..15731)
                     /gene="wzy"
                     /locus_tag="OQM34_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCS1451982.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(15751..16656)
                     /gene="rfbG"
                     /locus_tag="OQM34_001484"
     CDS             complement(15751..16656)
                     /gene="rfbG"
                     /locus_tag="OQM34_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCS1451983.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(16649..17539)
                     /locus_tag="OQM34_001485"
     CDS             complement(16649..17539)
                     /locus_tag="OQM34_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCS1451984.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17569..18825)
                     /gene="wzx"
                     /locus_tag="OQM34_001486"
     CDS             complement(17569..18825)
                     /gene="wzx"
                     /locus_tag="OQM34_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCS1451985.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(18841..19386)
                     /gene="rfbC"
                     /locus_tag="OQM34_001487"
     CDS             complement(18841..19386)
                     /gene="rfbC"
                     /locus_tag="OQM34_001487"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCS1451986.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19391..20269)
                     /gene="rfbA"
                     /locus_tag="OQM34_001488"
     CDS             complement(19391..20269)
                     /gene="rfbA"
                     /locus_tag="OQM34_001488"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1451987.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20328..21227)
                     /gene="rfbD"
                     /locus_tag="OQM34_001489"
     CDS             complement(20328..21227)
                     /gene="rfbD"
                     /locus_tag="OQM34_001489"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCS1451988.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21227..22312)
                     /gene="rfbB"
                     /locus_tag="OQM34_001490"
     CDS             complement(21227..22312)
                     /gene="rfbB"
                     /locus_tag="OQM34_001490"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1451989.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(22685..23578)
                     /gene="galF"
                     /locus_tag="OQM34_001491"
     CDS             complement(22685..23578)
                     /gene="galF"
                     /locus_tag="OQM34_001491"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCS1451990.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(23753..25147)
                     /gene="wcaM"
                     /locus_tag="OQM34_001492"
     CDS             complement(23753..25147)
                     /gene="wcaM"
                     /locus_tag="OQM34_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCS1451991.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25158..26378)
                     /gene="wcaL"
                     /locus_tag="OQM34_001493"
     CDS             complement(25158..26378)
                     /gene="wcaL"
                     /locus_tag="OQM34_001493"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCS1451992.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26375..27655)
                     /gene="wcaK"
                     /locus_tag="OQM34_001494"
     CDS             complement(26375..27655)
                     /gene="wcaK"
                     /locus_tag="OQM34_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCS1451993.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(27835..29313)
                     /gene="wzxC"
                     /locus_tag="OQM34_001495"
     CDS             complement(27835..29313)
                     /gene="wzxC"
                     /locus_tag="OQM34_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCS1451994.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29315..30709)
                     /gene="wcaJ"
                     /locus_tag="OQM34_001496"
     CDS             complement(29315..30709)
                     /gene="wcaJ"
                     /locus_tag="OQM34_001496"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCS1451995.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(30764..32134)
                     /gene="cpsG"
                     /locus_tag="OQM34_001497"
                     /gene_synonym="manB"
     CDS             complement(30764..32134)
                     /gene="cpsG"
                     /locus_tag="OQM34_001497"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1451996.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32327..33763)
                     /gene="cpsB"
                     /locus_tag="OQM34_001498"
     CDS             complement(32327..33763)
                     /gene="cpsB"
                     /locus_tag="OQM34_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1451997.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(33766..34989)
                     /gene="wcaI"
                     /locus_tag="OQM34_001499"
     CDS             complement(33766..34989)
                     /gene="wcaI"
                     /locus_tag="OQM34_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS1451998.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLVTAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(34986..35465)
                     /gene="gmm"
                     /locus_tag="OQM34_001500"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(34986..35465)
                     /gene="gmm"
                     /locus_tag="OQM34_001500"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1451999.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35465..36433)
                     /gene="fcl"
                     /locus_tag="OQM34_001501"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35465..36433)
                     /gene="fcl"
                     /locus_tag="OQM34_001501"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36436..37557)
                     /gene="gmd"
                     /locus_tag="OQM34_001502"
     CDS             complement(36436..37557)
                     /gene="gmd"
                     /locus_tag="OQM34_001502"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1452000.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(37583..>37848)
                     /locus_tag="OQM34_001503"
     CDS             complement(37583..>37848)
                     /locus_tag="OQM34_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1452001.1"
                     /translation="AHSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFV
                     APGVTIGDGTVVGARSSVFKSLPANVVCRGNPAVVIRERVETE"
BASE COUNT        10103 a         9498 c         8549 g         9698 t
ORIGIN      
        1 cagagatttt tatgacacgc gttcaattta aacaccacca tcatcctgac tagtctttca
       61 ggcgatgtgt gctggaagac attcagatct tccagtggtg catgaacgca tgagaaagcc
      121 cccggaagat caccttccgg gggctttttt attgcgcggt tgttaacggt tcagacaggt
      181 ttaaagagga ataagaaaat gacagacaac actcgtttac gcatagctat gcagaaatcc
      241 ggccgtttaa gtgatgactc acgcgaatta ctggcgcgct gtggcattaa aatcaatctt
      301 cacacccagc gcctgatcgc gatggcagaa aacatgccga ttgatattct gcgcgtgcgt
      361 gacgatgaca ttccctgtct ggtaatggac ggcgtggtag accttgggat tatcggcgaa
      421 aacgtgctgg aagaagagct gcttaaccgc cgcgcccagg gtgaagatcc gcgctacttt
      481 accctgcgtc gtctggattt cggcggctgc cgcctttcgc tggcaacgcc ggttgatgaa
      541 gcctgggacg gtccgctctc cttaaacggt aaacgtatcg ccacctctta tcctcacctg
      601 ctcaagcgtt atctcgacca gaaaggtatc tcttttaaat cctgcttact gaacggttct
      661 gtcgaagttg cgccgcgtgc cgggctggcg gatgcgattt gcgatctggt ttccaccggt
      721 gccacgctgg aagctaacgg cctgcgcgaa gtcgaagtta tctaccgctc gaaagcctgc
      781 ctgatccagc gcgatggcga aatggaagaa tctaaacagc aactgatcga caagctactg
      841 acccgtattc agggtgtgat ccaggcgcgc gaatcaaaat acatcatgat gcacgcaccg
      901 accgagcgcc tggatgaagt catcgccctg ctgccaggtg ccgaacgccc aaccattctg
      961 ccgctggcgg gtgatcaaca gcgcgtagcg atgcacatgg tgagtagcga aaccctgttc
     1021 tgggaaacga tggaaaaact gaaagcactg ggtgccagtt caatcctggt cctgccgatt
     1081 gagaagatga tggagtgatc gccatgagct ttaacacaat cattgactgg aatagctgta
     1141 ctgcggagca acaacgccag ctgttaatgc gcccggcgat ctccgcttct gaaagcatta
     1201 cccgcaccgt taacgatatt ctcgataacg tgaaaacgcg tggcgatgag gccctgcgtg
     1261 aatacagcgc gaagtttgat aaaaccacgg ttaccgcgct gaaggtgtct gctgatgaga
     1321 tcgccgccgc cagcgaacgc ctgagcgacg agctaaaaca ggcgatggcg gtggcagtaa
     1381 agagtattga aaccttccac actgcgcaaa aactgccgcc ggtagatgta gaaacgcagc
     1441 taggcgtgcg ttgccagcag gtcacgcgcc cggtggcttc agtgggtttg tatattccgg
     1501 gcggctccgc cccgctcttc tcaacggtat taatgctggc aactccggcg cgtattgcgg
     1561 gctgtaaaaa agtggtgctg tgctcaccgc cgccgattgc cgatgaaatt ctttatgcgg
     1621 cgcagctgtg cgatgtgcag gacgtgttta acgtcggcgg cgcacaggcc attgccgcgc
     1681 tggcgtttgg tacggaatct gtgccgaaag tggacaaaat cttcgggccg ggtaacgcct
     1741 ttgtcaccga agcaaaacgc caggtaagcc agcgtctgga cggtgcggcg atcgatatgc
     1801 ccgcaggccc gtcggaagtg ctggtgattg ctgacagcgg cgctacgccg gattttgtgg
     1861 cttctgattt gctttctcag gctgaacacg gccctgactc acaggtaatt ttactgaccc
     1921 ccgatgctga tatggcgcgt cgcgttgccg aggccgtcga acgccaactg gcagaactgc
     1981 cgcgtgccga aaccacccgc caggcactga acgccagccg cctgatcgtg actaaagatt
     2041 tagcgcagtg cgtggagatc tccaaccagt acggcccgga gcacctgatc attcagaccc
     2101 gcaacgcccg cgaactggtc gatggcatca ccagcgccgg ttcggtattt cttggtgact
     2161 ggtcaccgga atcggcaggc gactacgcct ccggcaccaa ccacgtgctg ccgacttacg
     2221 gttacaccgc cacctgttcc agcctcgggc tggcagattt ccagaaacgc atgaccgtac
     2281 aggaactgtc gaaagaaggt ttctccgcgc tggcttcgac cattgaaaca ctggccgccg
     2341 ccgagcgcct gaccgcccac aaaaatgccg ttactttgcg tgttaacgtc cttaaggagc
     2401 aagcatgagc accgtgacta ttactgattt agcgcgtgaa aacgtccgca acctgacacc
     2461 gtatcagtcg gcgcgtcgtc tgggcggtaa cggcgacgtc tggctgaacg ccaacgaata
     2521 tcccacagcc gtggagttta agcttactca gcaaacgctc aaccgctacc cggaatgcca
     2581 gccaaaagcg gtgattgaaa attacgcgca atatgcaggc gtaaaaccgg aacaggtgct
     2641 ggtcagccgt ggcgcggacg aaggtattga actgctgatc cgcgcttttt gcgaaccggg
     2701 taaagacgcc atcctctact gcccgccaac gtacggcatg tacagcgtca gcgctgaaac
     2761 cattggtgtc gaatgccgca cagtgccgac gctggaaaac tggcaactgg acttacaggg
     2821 catttccgac aagctggacg gcgtaaaagt ggtctatgtt tgcagcccca acaaccccac
     2881 cgggcaactg atcaatccgc aggattttcg caccctgctg gagctaaccc gcggtaaggc
     2941 gattgtggtt gccgatgaag cttatatcga gttttgcccg caggcatcgt tagctggctg
     3001 gctggcggaa tatccgcacc tggcgatttt gcgtaccctt tcgaaagcct tcgcgttggc
     3061 ggggcttcgt tgcggattta cgctggcaaa cgaagaagtc atcaacctgc tgatgaaagt
     3121 gatccccccc tacccgctct cgacgccggt tgccgacatt gccgcacagg cgttaagccc
     3181 gcagggaatc gtcgccatga gcgaacgggt ggcgcaaatt attacagaac gcgaatacct
     3241 gattgccgca ctgaaagaga tcccctgcgt ggagcaggtt ttcgactctg aaaccaacta
     3301 cattctggcg cgctttaaag cctccagtgc agtgtttaaa tctttgtggg atcagggcat
     3361 tatcttacgt gatcagaata aacaaccctc tttaagcggc tgcctgcgaa ttaccgtcgg
     3421 aacccgtgaa gaaagccagc gcgtcattga cgccttacgt gcggagcaag tttgatgagt
     3481 cagaagtatc tttttatcga tcgcgatgga accctgatta gcgaaccgcc gagtgatttt
     3541 caggtggacc gttttgacaa actcgccttt gaaccgggcg tgatcccgga attgctgaag
     3601 ctgcaaaaag cgggctacaa gctggtgatg atcactaatc aggatggtct gggaacacaa
     3661 agtttcccgc aagcggattt tgatggcccg cacaacctga tgatgcagat cttcacctcg
     3721 caaggcgtgc agtttgatga agtgctgatt tgtccgcacc tgcccgccga tgagtgcgac
     3781 tgccgtaagc cgaaagtaaa actggtggag ggttatctgg ctgagcaagc gatggatcgt
     3841 gccaacagtt atgtaattgg cgatcgcgcg accgacattc aactggcgga aaacatgggc
     3901 attaatggtt tacgctacga ccgcgaaacc ctgaactggc caatgattgg cgagcaactc
     3961 actaaacgag accgttacgc ccatgtagtg cgcaacacca aagagacgca gattgacgtc
     4021 caggtgtggc tggatcgcga aggtggcagc aagattaaca ccggcgttgg cttctttgat
     4081 cacatgctgg atcagatcgc cacccacggc ggtttccgca tggaaatcaa tgtcaaaggc
     4141 gacctctata tcgacgatca ccacaccgtc gaagataccg gcctggcgct gggcgaagcg
     4201 ttaaaaattg ccctcggcga caaacgcggt atttgccgct ttggttttgt gctgccgatg
     4261 gacgaatgcc ttgcccgctg cgcactggat atctctggtc gcccgcacct ggaatataaa
     4321 gccgagttta cctaccagcg tgtgggcgat ctcagcactg aaatgatcga acacttcttc
     4381 cgttcgctct cttacaccat gggcgtgaca ctacacctga aaaccaaagg taaaaacgat
     4441 catcaccgtg tagaaagcct gttcaaagcc tttggtcgta ccctgcgcca ggccatccgc
     4501 gtcgaaggcg acaccctgcc ctcgtcgaaa ggagtgctgt aatgaacgtg gtgatccttg
     4561 atactggctg cgccaacctg aactctgtga agtctgccat tgcgcgccac ggttatgaac
     4621 ccaaagtcag ccgtgacccg gacattgtgt tgctggccga taaactgttt ttacccggcg
     4681 tcggcaccgc acaagcagcg atggatcagg tgcgtgagcg cgagctgttt gatctcatca
     4741 aagcctgtac ccaaccggtg ctgggcatct gcttagggat gcaactcctg ggacgacgca
     4801 gcgaagagag caatggcgtc gacttgctgg gcatcatcga cgaagacgtg ccgaaaatga
     4861 ccgactttgg tctgccactg ccacatatgg gctggaaccg cgtttatccg caggcaggca
     4921 accgcctgtt tcaggggatt gaagacggcg cgtactttta ctttgttcac agctacgcaa
     4981 tgccagtcaa tccgtggacc atcgcccagt gtaattacgg cgaaccgttc accgcggcgg
     5041 tacaaaaaga taacttctac ggcgtgcagt tccacccgga gcgttctggt gccgctggcg
     5101 ctaagttgct gaaaaacttc ctggagatgt gatgattatt ccggcattag atttaatcga
     5161 cggcaccgtg gtgcgtctcc atcagggcga ttacggcaaa caacgcgatt acggtaacga
     5221 cccgctgccg cgcttacagg attacgctgc gcaaggtgcc gaagtgttgc acctggtgga
     5281 tctgaccggg gcaaaagatc cggctaagcg tcaaatcccg ctgattaaaa ccctggtcgc
     5341 gggcgttaac gtcccggtgc aggttggcgg cggtgttcgt agtgaagaag acgtggcggc
     5401 gttactggaa gctggcgttg cgcgcgtggt ggtcggttcc accgcagtga aatcaccgga
     5461 cgtagtgaaa ggctggtttg aacgtttcgg tgccgatgcg ctggtgctgg cactggatgt
     5521 ccgtattgac gagcaaggca acaagcaagt ggcagtcagc ggctggcaag agaactcggg
     5581 cgtttcactg gaacaactgg tggaaaccta tctgcccgtc ggcctgaaac atgtgttgtg
     5641 taccgatatc tcgcgcgacg gcacgctggc gggatctaac gtctctttat atgaagaagt
     5701 gtgtgccaga tatccgcaag tggcgtttca gtcctccggc ggcattggcg acattaacga
     5761 tgttgctgcc ctacgtggca ctggcgtgcg cggcgtaata gttggtcgtg cattactgga
     5821 aggtaaattc accgtgaagg aggccatcgc atgctggcaa aacgcataat cccgtgtctc
     5881 gacgttcgtg atggtcaggt ggtgaaaggc gtacagtttc gcaaccatga aatcattggc
     5941 gatatcgtgc cactggcaaa acgctacgcc gaagaaggtg cagatgaact ggtgttctac
     6001 gatatcaccg cttccagcga tggccgtgtg gtagataaaa gctgggtatc tcgcgtggcg
     6061 gaagtgatcg acattccgtt ttgtgtggcg ggtgggatta agtctctgga agatgccgcg
     6121 aaaattcttt cctttggcgc ggataaaatt tccatcaact cccctgcgct ggcagaccca
     6181 acattaatta ctcgcctggc cgatcgcttt ggcgtgcagt gtattgtggt tggtattgat
     6241 acctggtacg acgccgaaac cggtaaatat catgtaaatc aatataccgg cgatgaaagc
     6301 cgcacccgcg tcactcaatg ggaaacgctc gactgggtag aggaggtgca aaaacgcggc
     6361 gccggagaaa tcgtcctcaa catgatgaat caggacggcg tgtgtaacgg ttacgacctc
     6421 aagcagctga aaaaagtgcg tgaagtttgc cacgtcccgc tgattgcctc cggtggcgcg
     6481 ggcaccatgg aacacttcct ggaagccttc cgcgatgccg acgttgacgg tgcgctggca
     6541 gcttccgtat tccacaaaca aataatcaat attggcgaat taaaagcgta cctggcaaca
     6601 cagggcgtgg agatcaggat atgttaacag aacaacaacg tcgcgaactg gactgggaaa
     6661 aaaccgacgg tctgatgccg gtgattgtgc aacacgcggt atctggcgaa gtgttaatgc
     6721 tgggctacat gaacccggaa gccttagaca aaaccatcga aagcggtaaa gtcaccttct
     6781 tctcgcgcac taaacagcga ctgtggacca aaggcgaaac ctcgggcaat tttctcaatg
     6841 tggtaagtat tgccccggac tgcgacaacg acacattact ggtgctggcg aatcccatcg
     6901 ggccaacctg ccacaaaggc accagtagct gcttcggcaa caccgctcac cagtggctgt
     6961 tcctgtatca actggaacaa ctgctcgccg aacgtaaata tgccgacccg gaaacctcct
     7021 acaccgccaa actgtatgcc agcggcacta aacgcattgc gcagaaagtg ggcgaagaag
     7081 gcgtggaaac cgcactggca gcaacggtac atgatcgctt tgagctgacc aacgaggcat
     7141 ctgacttgat gtatcacctg ctggtgctgt tgcaggatca ggatctggat ttaacgacag
     7201 taattgagaa cctgcataaa cggcatcagt gagttacggg ctgagcaggt gtgatgttgt
     7261 tgtcacaccc ggagccattg cccgatgcgc aaacttatcg tgcctacaca ttatctcaac
     7321 acacgtaggc cggataagat gcgccagcat cgcatccggc aaaaaaaacg ggcatggtgt
     7381 caccaccctg cccgtttctc ttaaatgcac aataatatta cttcgcgttg taattacgca
     7441 gagcgttacg ccccaacaca atccccgcgc caaccatgcc gcccagaagc actgccagaa
     7501 tcaaggtgat tgcctttttc ggactatcgc gacgaatagg taacgtcggt ttcatcacat
     7561 agcggtaagc atgaatatca agatcatcaa actttaattt ttcaatatcc aacaggtttt
     7621 gacgtgtctg atagtagttt ggtgagaaca ccaacggacg agtcgcttca tgcttaatca
     7681 tcgactccag cgcttcgctc cctagaagga acaacgtatc ttgcgtcaca tcttcagtct
     7741 gctgaacctg tggctttgtc acctgcgcct gattcgcata ctgcaacgct tcctgaatct
     7801 gacggatacg cagatctttc tgctcctgcg cgaccacttc ctgggttctt aaagagtcct
     7861 gcaagttttt ccgtcccaga gcaatgttgt ccttgagatc cttttctagc tcttgattca
     7921 ctttatcatc aacttgctga atgtattggg ccaacttcat ttgtgcgccc tctgcagttt
     7981 gcccaacata agaaacagtc aatggtaatt gctggttctt aacagaaggt tcgatggtaa
     8041 gtttttctgg ctcttcctga ttatccagcg tttctgctaa tgcagagaag gcagaactga
     8101 agcgaccaat taacgtctcc tgcaaatccg atactttcgg tgcagcctga ccatagataa
     8161 cattcatggc attgttatag ccagcaattt gccccacgtc gggctgagtg ataattgctg
     8221 ttgacgtcca tttctccttc gctactgcca aataaccaat agccagggca atagccacaa
     8281 tgacggaaat gataattgtc atcttgccac gccacaactg cactagtaaa tcaatcaaat
     8341 caatctgttc cgggtcatgg ttttgcccag aaacattatt attttctact ctcatcatta
     8401 ccctaactga cggaaatttc caagtccgca tagtttagcg ataatccccg tgaatgctat
     8461 aggataagtg ataaaaataa tgaattattg cggaggtaac aaaagacaat cagggcgtaa
     8521 atagccctga taacaagatg ttagtcgctg ccaaagagat cgcgggtgta gaccttatct
     8581 gccacatcct taagctcttc tgccatacgg ttggagataa tgacgtcggc ttgttgtttg
     8641 aaggtggcga gatcacgttc caggcgagag ttgaagaatg agtcttcttt catcaccggc
     8701 tcgtagatga tcacttcaac gcctttcgcc ttgatacgct tcataatccc ctgaatagaa
     8761 gacgcacgga agttatctga cccgctcttc ataatcagac gataaatacc caccacttgc
     8821 ggtttgcatc gacaattgcc gagatcaggt tattcggcac agactggtag ttcgccagta
     8881 actgcttggt atctttcggc agacaataac caccataacc aaacgacgga ttgttgtaat
     8941 ggttgccaat acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta
     9001 aactttctgc ataactatcc agttcattaa agtatgctac gcgcatcgcc agataggtat
     9061 tagcgaaaag tttaatcgct tctgcttcag tggagtcggt aaacagggtc gggatatttt
     9121 gcttaatcgc gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac
     9181 cgatgacaat acgtgacgga tggagattat cgtaaagggc tttaccttcg cggaggaatt
     9241 ccggagagaa aataatattt tcagtacgat atttcttatg catcgctgcg gtaaaaccaa
     9301 cgggaaccgt tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa
     9361 ttactgattc tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag
     9421 tggcgatgat gacataatca gcatcccggt aggcttcatt tttatctaat gtggcattaa
     9481 agtgtatttt atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc
     9541 gatcattcag catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt
     9601 gtgcgattag aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca
     9661 tttcatgctc tcagaattaa cttaactgtg aatcatgatg tttttagcat cgtgataaga
     9721 tgctaaaagt tttaacgcta cgttgtcaga tggtcaacgc aacgaaaaac aattcagatt
     9781 ttctttataa gaatagctga tgtttattaa gttaatatta agcagtgaaa tttagtctat
     9841 aagatatttg gcaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttattgttgg
     9901 ttaaatcaga ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat
     9961 aagtatgcgc cccgaaatag tcacgctgtg cctggatcag gttcgcaggc agaaccgcgg
    10021 cacggtagct atcgtaatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
    10081 gtactgcata agcgacgaca tcgcgcagcg cctgctggta gtcatcggca atttgcttga
    10141 agtacggggc cagcagcagg ttagcgatct gcggattttc ggcataagca tcggtgattt
    10201 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
    10261 agttcagatc ccaattgtat tcttcagacg cagcacgcag ctgagagaag ccctgagcgt
    10321 aagaaacgat tttgcccaga tacagcgcac tgcgaacttt ttcgatgaac tcagccttgt
    10381 cgcctgctga ctgagcttgc ggaccagaga gaactttaga tgcggcaacg cgctgatctt
    10441 tcagagaaga gatataacgt gcaaacacag actcggtaat cagcgacagc ggttcgccga
    10501 gatccagcgc gctctggctg gtccatttac cggtgccttt gtttgctgct tcatccagaa
    10561 tcacatcaac caggtagtta ccgtcttcat cttttttggt gaagatatct ttagtgatgt
    10621 cgatcaggta gctgctaagt tcaccgttat tccactcggt gaaagtctgg gccagttctt
    10681 cattagatag attcaggcca cctttgagca gagaatacgc ttcagcaatg agctgcatat
    10741 ctccgtattc aataccattg tgaaccatct tcacataatg gcctgcgccg tcggcaccaa
    10801 tataagtaac gcatggctcg ccgtcttctg ccacggcagc gattttagtc aggataggcg
    10861 caaccagctc ataagcctct ttctgcccgc caggcataat agaaggacct ttcagcgcgc
    10921 cctcttcacc gccggaaaca ccggtaccga tgaagttaaa gccctctgct gaaagctcac
    10981 gattacgacg aatggtgtcc tggaagaagg tattaccacc atcaatgatg atgtcacctt
    11041 tatcgaggta tggtttcagg gaatcaatcg cagcatccgt gcctgcacct gctttcacca
    11101 ttaacaggat gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag
    11161 gagccagttt cttgcctgga ttttcagcaa tcacttcttc cgtcttttcg cgggagcggt
    11221 tgaaaataga gacggtataa ccacgacttt cgatgttgag cgcaaggttg cgccccatca
    11281 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt cttctatatt
    11341 attaaccgtc tattaaccgt tatattatta accgtcgtga ttttcatctt ctaatcaagg
    11401 aatacatatg gttacagcat gcttcttact ttatagcgat aagtatgcca ttgggtgtat
    11461 ccataagaac tttgaatttt ggatactttt ctggattaaa attagcatag attcgtttag
    11521 atataacaat aaacgaacta ttaaaagatt tcatcttctc tattaaatca tcctgtggga
    11581 ttacctcaca ctcgggatta ctaatagtcc aatcactata tcttgctaat ccaaagacgt
    11641 caccaattgc catcccttta tagaagtaaa taccatcctc caagcctaat tggataagat
    11701 tatctccata taactcacta tattcacttg ctttcttcat cagttcatag ctatagcgac
    11761 tttctaagcg tgaattaaaa ttcaagtatt cagaataaaa atatttgaat tcaaaataca
    11821 acgatcgccc cgaaaaaaaa taatacacac aaggataaaa tatcgtccca acttgtaatt
    11881 tgtaatgttg atatctttta ttaaacttgc aattgagatc gcaacctgca atactgacaa
    11941 tgggtaaatg ggtgataaat acctgtcacc ttgcgtaaca gaaaaccaaa ataagacata
    12001 tgttaaaaca atcgagaaaa atacccaaag tattttatta ttcctaagtg taaagacaga
    12061 cagtatagca taaaaaacaa catcactgcc aactttatta attgcttgtt gcaaatctaa
    12121 tgtggcagga cttataccat gtcgagcttg ttctgctgtt tgaacttgta gatcaatttc
    12181 attccatagg tagtgaccaa atatatttcc tccagcagga ctaaaaggat caccagagat
    12241 ccaaaaacta cggacatacc aataaatgcc accaagaatt gttgccaagc taaaaataag
    12301 aaatgtctgt atgtttctct ttttcgctat caatataaca aatatgatgg ctccatatat
    12361 ggcaccaaat agttttacgc cacaagcaat tccaataaaa aaaccacata aaaaaattaa
    12421 ttcattttgt gttttattgt tttctgagcg aatatatata agaatactgg ctgcagcaaa
    12481 acaaagatag cgttaaatca atatatgcat aaccaagatg atctttaagt ggattgatag
    12541 taaatagtag gattgtagca ataatgcctg gataataagt tttcgtaatt tcccatgaac
    12601 agccaacaag tccacaggct attacaaaaa aacagatatt tgctaaaaac tgagctaacc
    12661 ttacatctcc taactgtaaa cctaaagcca ttaaaagatc tgcattttgc gggaacagag
    12721 gaaaacgcaa atattgttca acaacaattg catggtgctc aactaatgaa cgagccaaag
    12781 gtaagtgata catagtatca tcccaatcac cgggagctcg cagggacgat aacaatgtaa
    12841 ccacaaaaat caaaacaaga aataaaataa caaacagcgt actgaactca atattattta
    12901 ttgccagcca tctaaattgc tgacgtttaa taatgcttaa aacccaacac accgtaaaaa
    12961 ttaaaaagat agcaatctta ttaaattgcc cgaccatgcc cagaaagtaa attatcaaaa
    13021 tatttaaaga catccctatg cataggcaat aaaaaataaa gaaaatttgt taagtcctgc
    13081 caccccctcc ttaaaaaaag atgacaatac aatattacca atgacaaaaa atgtcattcc
    13141 aaaagcaaaa caatacacaa catacaaata caacggcaat tcaatcatag taaaatactc
    13201 tatttacatt atcagtttat agtttttttc agaaagataa aataatgcct tcatttcacg
    13261 tctagttgtt gtatttgaat gcaaaataat gccacataca aatgccatta ttgacgataa
    13321 catgatggag gcagttaaaa aggcagtcgg aaatcttctc accaaacctg tatcaatata
    13381 atcaacaatg atgggcaatc ctaaaattat agacaaagca gcaagtaaaa cgccgataat
    13441 actaaaaaag tatagtggcc gttcaagcga gtaaagttta attattgttt tcagaatctt
    13501 gaacccatca ctccaagttg atagtttgct cacactccct tcagatcgct cgccatattt
    13561 tgtattaacc tcaccatact tcatacgtaa ttctaaagca tgaattgtaa gctcagtttc
    13621 gatttcaaat ccccttgata gacatggaaa agatttaaca tagcgtctgg aaaatacacg
    13681 ataccccgac aacatatcag taaaattccc tttaaagata gtctgaacag taccagtcaa
    13741 cagtttattt cctcagcgat gcccttttct gtatgtattt tgatctccat tctcttgacg
    13801 acatcctaca accatatcca attgattttt tatgagatgg ttaatcattt ctggagcaga
    13861 acttgcatca taagtgtcat ctccatcaac cattagataa atatccgcat ctacatctga
    13921 gaacatacgt cttacgacat tccccttgcc tttcagagga acagagtgaa ctgttgctcc
    13981 agcatcctct gccacttgtg ctgtactgtc agtcgaatta ttatcaaata catatataga
    14041 agcagttgga atattcgttt tgaaatcatt tatgactttt gctattgcct tctgctcatt
    14101 ataacaagga ataattacag cgatattaaa agagtgatac ataagcccct catataaact
    14161 atttagctaa tgtgcaagta attgtgtttt taacaaatcg tttactaacg aaaaatgaaa
    14221 tattttgatg cgcaatatcc taaaaccaaa ctaaaaagag aaaaggtgaa caatgtaaat
    14281 attggagata aggctaagag atcaaataaa acgccgaata aataactcat cgcccccata
    14341 aaaaatataa atataaaata tctagccttg gttgcagata ctttaaatga gcatcttgca
    14401 tttgcaaaaa aagaaaaggt tgctgcacat ataaaaccaa caatgttact tcggccttga
    14461 ccaaaagcta aattattata acacgcataa aatacccccc aatgtaaagc agtattgaca
    14521 attccaatta aaaaataact aaaaaaagaa ctggtcaata acttccctat ttttaacatc
    14581 ctttattttg ctccagaagt gaggttatta ctaatttgga tattttctat agaaaatacc
    14641 cataaaacta caacgcctat aataaaaatt cccatgggtg taaaaagaaa tggattagtt
    14701 atagaaacgg taatactgaa aaaaagtatt aacgacatca ttaatgatgt ttttgtacta
    14761 tttaaaaaat agcctttcaa aaagataagc aacataggaa ctaatgatat aaatacacca
    14821 ataacgcctg ttttctgaag tatctcaaga ggtactattt caatccttaa tcgatctaat
    14881 ataaatgaac caaatcctct tccaaacaag aacgtcgcta aatcaacatt tttataataa
    14941 aaatataaat cattaaatct aacagagtca gaatcttcac tgcgtgatcc gaccatgtaa
    15001 agcataatta taaatagaac gctgcccaat attattataa tagtatttaa atatagctta
    15061 tgtaatttaa aactggctaa taacagtgat aatgttgtaa aaagtaatac acctcttgat
    15121 tctgtcaata atatcgcaag cactattatg agacatttta aatatttttt gtcgacaaaa
    15181 ctgataatcg caccgacacc aaaaaagtag aaggatttgg aaaaaaaagc cccatcggga
    15241 cgaaaaaaga actcgctagt caatgataag tgttcataaa ttaaattaaa atttaacaaa
    15301 ccgaaattca acaaaattat gtatgaaata tataacagtg atccatataa tgcaaatgca
    15361 ttaactattt taataaaaat ctcacatgaa aatactttta aagtatatct tagtgtaaat
    15421 agatatataa gtgacagcat aaaaaggtat ggttgcacat caaacagtat atcatttatt
    15481 ttattaccag ctaataagcc gacaaagaag ccataaaaag gaaataaaag caaccatata
    15541 aaaagactga cacatttttg ggtgattgaa aaattgaatg taactaaatt aaaaattaaa
    15601 agtataaaac taaatacaaa taataaatat cgcaaaggga atattcccaa tggctccagt
    15661 aatcttccac caccaccaat aatcagttca atacataaaa atgttataaa aattttattt
    15721 atattattca tattcgtaag gtgatgtttt ttaaaatatg tttaaaatca acattaataa
    15781 aatataaatt ttatcagcct ttttccgcct taacttccaa aaacttaaag ccaagatcaa
    15841 attgcctata taacctttac ccaattgatt attcacaaag ctatcatcag ctaaggcatt
    15901 agctatttta gttacttctt ttcgatacca cttgtttctc accaaaccaa gccgtttgta
    15961 ttttgcttta aaactaatgt ttgctcccac ttggttgttt tcatgctgtc tgtacagcat
    16021 tgttggtttt ctatcaatat accacgaata atttctcgtc cgcgcaaatg aatataaaaa
    16081 ccaatcatgc agacagatat cttgtgcagc atttttatta tttatgatga acttcttaaa
    16141 ttcaataagc gtttcttttt ttataacata tgtacacccc ggacctgctg cttcaaaaaa
    16201 ataatcgaat tgagtttgcg actgagcctt atcaactaaa ttttttcgcc ctgatggata
    16261 atatgcaatt acattactgg aataacaatt cgcattatta aataccaagc agtcaattgc
    16321 attttttatt ttatctttat accaaatatc gtcctgatca ctaaaacaga tataatcata
    16381 gttttcaaga tcgacatctt taattaatct ataaaaattt ttacctgccc ccccaaactt
    16441 attgacgctt ggaagtatat ttataattga gggatatgat aattgaaatt cttcacaaat
    16501 atttaatgta ttatcagttg ataaatcatc tgatataaaa attgagatgt ctacatcttt
    16561 ttgttcaata atacttttta attgttcctc aatccagcat tcgccattat acgtagccat
    16621 aagtacagct actctttttt gatcagaact atgcattata gcgaccgccc ccagtaattc
    16681 ctcttattcc atccagcgta gccaaaatat gttttttcat atattcaatt ttattttttt
    16741 gagttgcgat aattaaagtc gtatgaatta aagaaaaaac aacttcccta atagagagta
    16801 aaagaggcac atgatttttt ctaagcaaaa gaaatgcatt tcttactcga taataacgac
    16861 gtactggcga gtgaattggt atgttaatcc cacagatttt tactgactta tccccaatag
    16921 catgaaccat ttctatatca ggaatgatat gaactaatat gccatagttt aagcatctaa
    16981 gacaccattc tgtatcgaca taatcaataa aaagttcctc atccatcatt ccaacaatct
    17041 caaaaacttc ttttgaaacc atagttccgg atgagattgt tactgaactt ttaaacggtg
    17101 tctgtccttc agtgacatga attttctctc gaagaccatt ttttttgata ttacaaatag
    17161 gataataatg tgacttatct ctatcaataa aaactgggcc agctattttt gcattttcat
    17221 tatccatata gcaggaaagt tttttgataa actcattgcg aatacggcta tcttgatcaa
    17281 aaaaaattat cttctctgcc cctttttcaa gagaatatcg aatcccaata ttttgtgctt
    17341 ttgctatacc ttcattccta ccaagacaaa ttaaatgaac tttttcaatg tctgctaatt
    17401 tatctttcaa ggtgaaatta gtcctattat cgactactac aacgtaacaa ttttgctcct
    17461 ttagttcagt gatcagtgca ttcagatttt taagttcggg attatatgtc acaatgacag
    17521 cgtaaatatt actattcatt atccaagtga ctcagtaatt ggttaatttt agttttcata
    17581 tacagaacac attctagtat aaagccattg taataacaga gcatctaaaa ttacacgtat
    17641 gctccaggat attgcggcac ccagtaaccc atatttttcg accataaaat acaaaagggc
    17701 taaataagga accaattcag cacaatgaat caaagcagta attttagatt ttcctaatgc
    17761 ctggattgca gagaatggta tcattgccag cgcattgaag aaaaaaccga tcaataacac
    17821 atttaatata ttaaaggaat taatttgata attctcacca aaccatattt tcaatacaag
    17881 ccctgaatat aacaagccta tgattatcac cggcaaacag attagaaaca ttaaaagcaa
    17941 tgatttattt acattacgtt taaattcttt aaagtctttg atgttactta accttggaaa
    18001 cactgccctc ccgattgctg caggtattat tcctaatttt agaataacct ccgctggtgc
    18061 agaataaaat gcaactttat ctgcccccat aatatttgac actataaatc gatcaaaata
    18121 caccatgacc ggacttatga tattacttac tgtcatccaa ccgccaaaga aaaagagtcg
    18181 tttaaaggtt ttataacaaa aatgaacccc cgaaatttta atatcattcc tgacataata
    18241 ggcagagact aaaatcgaaa taactcttgc aaatattaaa ccagcaaccg ctgccgacaa
    18301 tgtagcacta taaaaaacaa atatggccgg aattcccgcg atgcaagagc ttgatataga
    18361 tttttgaata tttacaatgc caaatttttc atccccctca agaatggctg accataattg
    18421 atttagaata aataatggaa tgcaaatagc caacagttta aatgctagtt gtatatcact
    18481 atgttcaaca ccagaaattt ttagataatt aacaattcca tcagagaaaa tcaataataa
    18541 aaaagcacca aagcatgaaa atagcaccaa aaaagatgta cttgttgaaa ttaccttggt
    18601 tctttcatga tgattatcac gatgaattgc aatttctcta ataacagagc gagtcaggcc
    18661 tacatcaaaa attccagcat acccaacaag tgcaattgca attgtataaa taccgaaacc
    18721 ttcaggcccc aaacctcgag cgagaaatcc tagcgagggg atagcaatta aagttggtat
    18781 tgcatagcca aaaaggttcc agacactatt ttttattatg ctcattatta catttcttta
    18841 tcatataaaa taatcagcat ctacaaattt ttgaccatta atatctttat cagaaagcaa
    18901 tggattttga accggccatt ctatatttat acttttgtcg ctccataaaa tcccacgctc
    18961 ttgcattttt gaatagaaat tatttgtttt gtagacaaat tctgcttcat cactaagaac
    19021 gcaaaaccca tgagcgaatc cttccggtat ccataattga cgcttattct ccgccgacaa
    19081 atttacacca acccacttgc caaatgttgg tgatgatttc cgaatatcaa cagctacgtc
    19141 aaaaacttca ccgacaacac agcgtaccag ttttccttga gcataaggtt ccagctgata
    19201 gtgcagtccg cgtaacacac ccttagttga tttagaatgg ttatcctgaa caaactcaac
    19261 ctttcgtcct acagcctctt cgaaaacttt ctgattgaag ctttccataa aaaagccccg
    19321 ctcatcacca aatacttttg gttcaaaaat tagcacatca ggaatttcag ttttaattac
    19381 gttcatttta ttaataacct ttaatcattt tcagcagata ctgaccataa gcatttttct
    19441 tcagcggctc ggctaatacc ttcacttgct cagcatcaat aaagccttta cggtaagcaa
    19501 tttcttccgg gcaggaaact ttcagcccct gacgctcttc aattgttgca atgaagttgc
    19561 ttgcttcaat aagactttga tgtgtccccg tgtccagcca tgcataacca cgccccatca
    19621 tagcgacaga caaacgtcct tggtccatat aaatacggtt aatatcggta atttccagtt
    19681 cgccacgtgc agatggttta aggtttttcg ccatttctac aacgtcattg tcatagaaat
    19741 aaagcccagt aaccgcatag ttactttttg gttccagcgg tttttcttcc agactaattg
    19801 ccgtaccgtt atcatcaaat tccaccacac cgtagcgctc aggatcattt acgtgatagg
    19861 caaaaaccgt tgcgccactc tctctgttaa cagcggtatc cattaatttc ggcaggtcgt
    19921 gaccatagaa gatattatca ccaagtacca gcgcacaatc atcaccacct ataaattctt
    19981 caccaatgat aaatgcttgc gccagaccat ccggactcgg ttgtacttta tattgcagat
    20041 taagccccca ctggcttcca tcaccaagca attgttgaaa acgtggtgta tcctgcggcg
    20101 tactgataat cagaatatct cgaatacccg ccaacataag cgttgaaagc ggataataaa
    20161 tcatcggttt atcataaatc ggtagcagtt gtttgctcac agccattgtc acaggataaa
    20221 gacgagtgcc ggaaccacca gccagaataa tacctttacg cgttttcatt tcaccattcc
    20281 ttttaattca tctcgctcta gcatcatgag cgagatgcaa taatttgtta aattgccgtg
    20341 gtcgtaaata attcgttgag catacgtttc acgcccacct gccagtcagg caagacaagc
    20401 gcaaagttct gctgaaattt ttcggtattg aggcgagaat tatggggacg acgtgctggt
    20461 gtgggatagg ccgttgtcgg cacggcatta agtttgttaa gagcaaggtt gatccccgct
    20521 ctgcgcgctt cttcaaatac cagagcggca taatcgtgcc aggttgttgt accaccagca
    20581 accagatggt acaaaccagc aacttctggt ttatttgcgg ccacgcgaat ggcatgagcg
    20641 gtgcaatcag ccagtaattc agcccccgtt ggtgcgccga attgatcgtt tattacagcc
    20701 aactcttcgc gttcttttgc caggcgcaac atcgttttgg caaagttatt tcctttacca
    20761 gcgtataccc agctggtacg gaaaataaga tgctttgcgc aatgttcttg taacgctttt
    20821 tccccagcta gcttggtttc accgtagaca tttagcggtg ctgttgcatc cgtttccagc
    20881 catggcgtgt caccgttgcc tgggaaaaca taatcagtgg agtagtgtat aacccaggct
    20941 ccgacttcat tagctgcttt tgcaatcgct tcgacgcatg tcgcgttaag taattgcgcg
    21001 aaattcggtt ctgattccgc tttgtctact gcagtgtgag cagcagcatt aacaataaca
    21061 tcggggcgaa tttttttgac ggtttcagcc acaccttcgg ggttgctgaa atctccacaa
    21121 taatcagtgg agtgaacatc gagcgcaatc aagttaccca gaggagccag agcacgctgc
    21181 agttcccaac ccacctgccc tgttttgccg aaaagcagga tattcattac tgacggccct
    21241 catagttttg ttcaatccac gactgataag caccgctttt cacattctca acccaatttg
    21301 tattagccag ataccattcc accgttttac gaatcccgct ctcaaacgtt tcttgcggtt
    21361 tccagcccaa ttcgcggcta attttatcgg catcaatggc gtaacggcgg tcatgccccg
    21421 ggcgatcggc aacataagta atttgctcac gataagattt ctctttcggt acaatctcat
    21481 ccaacaaatc acaaatagtg agcactacgt cgatgttttt cttttcgtta tgtccaccaa
    21541 tgttataagt ttcacccgct ttaccttcgg ttacgacggt atataacgca cgagcatgat
    21601 cctctacata caaccagtcg cgaatttgat tccctttacc ataaataggt aatgccttac
    21661 cttccagagc attcagaata accaatggaa taagtttttc cgggaagtga taaggaccat
    21721 aattgttcga gcaattagtc acaatggttg gtaaaccata agtccgcttc caggcacgta
    21781 ccaaatgatc gctggaagct ttagacgcag aatacgggct acttggcgcg taagctgtcg
    21841 tttccgtaaa tagcggtaac gcttcgttat tatttgcttc atccggatgg ggtaagtcac
    21901 cataaacttc atcagtagaa atatgatgaa agcggaaact ttttttcttt tcatcattca
    21961 gagcagacca gtaattgcgc gccgcttcta aaagaacata agtccccaca atattggttt
    22021 caataaatgc cgcagggccc gttattgaac ggtcaacgtg gctctctgcc gccaggtgca
    22081 tcaccgcgtc tggctggtgc tgtgcgaaaa tacgcgacat ggctacagca tcgcagatat
    22141 ctgcatgctc aaaagcataa cgttcagaat cagaaacatc agcgagtgat tccaggttcc
    22201 cggcgtatgt taatttatcg acattaacaa cactatcttg cgtattattt attatgtgac
    22261 gaacaacagc agaaccaata aaccctgcgc caccagtaac aagtatcttc acttttttat
    22321 tccatatagc tatagagcat gctgtgaaat agactgctcc agatttgatt aatagaggta
    22381 ttaatacacg ctaccgcccc tggcttaaca gctaccagtg cactgcatgc atctcaacga
    22441 tgtggcgagc gttagccact cgcgccaaat ccgaaaaatt caaacgttaa ttgtcttacc
    22501 aaacagccgt agaaacaagg aaaatcctgg aaaaatttga ataatatgag ctatgtaact
    22561 tcttgttttt ataggcttta taaaaacggc aacggcagga aaattcaatg agcaaaactt
    22621 atgccacgaa tcttcactgc cgttataatt ttcttatcaa ccgttacatc cggtcagatt
    22681 ttcattattc gcttaacagc ttctcaatac ctttacggaa cttcgcccct tctttcaggt
    22741 tgcgtaggcc atacttcaca aacgcctgca tatagcccat ttttttgccg cagtcgtaac
    22801 tgtcgccggt catcagcatt gcatcaacgg attgtttttt cgccagctcg gcaatagcat
    22861 cagtcagctg aatacgtccc catgcaccag gctgagtacg ttccagttcc ggccaaatat
    22921 cggcagaaag cacatagcga cctacggcca tgatgtctga gtccagcgtc tgcggctgat
    22981 ccggtttttc gataaattca acaatgcggc tgactttacc ctcacggtcc agcggctctt
    23041 tagtctggat gacggagtat tcagagaggt cacccggcat acgttttgcc agcacctggc
    23101 tgcggcccgt ttcgttgaaa cgtgcaatca tggcagcaag gttgtaacgt agcgggtcgg
    23161 cgctggcatc gtcgatcaca acgtctggca gtaccacgac aaatgggttg tcaccaatgg
    23221 caggtcgcgc acacaaaatg gagtggccta aacctaaagg ttcgccctga cgcacgttca
    23281 taatgatcac gcccggcgga cagatggact gtacttccgc cagcagttga cgcttcacgc
    23341 gctgctcaag gagtgattct aactcataag aggtgtcgaa gtggttttcg accgcgttct
    23401 tggacgcgtg agttaccagg aggatttctt tgatccctgc agccacaatc tcgtcaacaa
    23461 tgtactgaat cattggcttg tcgacgattg gtagcatctc tttgggtatc gccttagtgg
    23521 caggcaacat atgcatcccg agacccgcta caggaataac tgcttttaaa ttcgtcatta
    23581 tttcatccac ctgtaaaatg gttgctgaat tatagcttgt tcgatttttt tcgccagcat
    23641 caattaccct gaattgatta ctgaattact tgtgatgtta cgccgcttcg ttgtggattg
    23701 cagtagcatt gttcctaagt atggctccat ttttccagga atggtcgcaa atctactccc
    23761 tccgttccgg caatctaaag ttaatctttt ccacattaac aatatggtga ttaatcctgt
    23821 cgatatcgac ggagctttgc cctctttcat tcaccgcatg aacatttgca agagacagca
    23881 gtgtttcttt tttcgccata aagacgccgc gaacgtcttt gcgcatgtcg aagttcatgc
    23941 tcaatgctgg tccaactgag gattcctgca tcacattgat atttcgcata aaaagatgtt
    24001 gcggcttgtt gtgtaactcc agcgaggcac gcttcatctc aatgttagtt agcgacacaa
    24061 aggagacggc attcccggcg gagatttgga tgccgcgcaa tttataagca agatgggtat
    24121 tatccagttg aatattattc actcggaaat tttgcggtat cgagagatat ttgcctttaa
    24181 ttaccccata gccgattaac atcccggcgc tattaatcat ttcaatatta tcaatcacga
    24241 aattgtcaca accgtaaata gcgactgtcg cgttatcaat gcccgctttc ttactgaaat
    24301 ccggcgtgat attgcgggca ttgatattac gaataacaaa atgtttacca ttttcaacat
    24361 ggatcaactg ccgacaatcc gatcccgtga tattcgccac gacaaagttt ttcactgcct
    24421 ggtcttccgg gtagttgtta tcataagtgc ttcccgcaag gcctatgccg atgccccagt
    24481 tgattttgcc gttggtacag ttgatacgct cgatgacatg gtcagatatc aaaatatcac
    24541 tgtcgttaat tgccacgttc cattcaatgg cgtcgccctg taagtcgctg aacttacagt
    24601 tggtaatgtt ggcaccgata atctggttat gaaatccctg gcgtaagatg gcgtaattag
    24661 cgtggctgac agtgaggtta tcgatggtca ggttgcgcat gacccgtttg tttttgccgc
    24721 caatataaat ctgcattacc gggccaaagc cgctcatcgc cagccctttg atggtgcagt
    24781 cagaaccacg cacatccagg gtaatgttat gcataccgcc gccctctccc cctgtcacct
    24841 ggctgccgtc ctgtaagaca aatcgccctc tgccgttgcc gcgcaggctt ccaaggatgt
    24901 gtaacgtttt gccgggaggg atgaagatgc cggtgttgat attgtcacaa acgaatccgg
    24961 caggcacgac gaccgtttgc ccttcgctga aggcttgttt aaatgaggcg atccagtcat
    25021 gtgggttgta gtcgttaatg ttaacgcttt gtcgggcggg aagtgcgcga gcgaaagggg
    25081 tatggaggaa ggcaagcgcc gagcttgccg tcaggaaggt gcgtcgggag agtgttttaa
    25141 atggcatacg ttctcctcta taaagcctgc agcaagctgg cgagttctcg attaatcacc
    25201 tgctggttaa aatcgtgttc gactttttcg cgcgcacgtt tgacgaccgg agccagttcg
    25261 tcggtatcca gttggctaaa cgccgccaga cgttgtgcca gtgcgcgagc atcgttctca
    25321 ggcagcagcc agccggattt atcggcctcc accagttcag gaattccgct atgcagagta
    25381 gaaaccaccg gaataccgac cgccatcgct tccattagcg ccaccggaat gccttccata
    25441 tcgccatccg cgcccgttac cgatggcaac aggaagacat ccgcgtcgtc gagcatctcc
    25501 ttcacttcat ggctcggttt aaagcccggc atctctacca catcttccag ttgatattgt
    25561 tcgatgaggg tgcgcaggcg tcgttcccac aggccaatgc cgaggatgcg atagcgaaat
    25621 gccacgccct gctctttcaa ctgacggcag gcttcaatcg ccacatgcag gccttttttc
    25681 tcggttaagc gtgcgacaga tatgatttcc agcggcgttg cgggcgcttt caccggacgc
    25741 gggctaaagc gcgtcatgtc cacgcccata cgcgatacgg cgattttttc tctggggcag
    25801 cccatttttt gcagccttcc ggcccacaga tcgcttatcg gtaacatcag gtcgccacgg
    25861 cgaaacagtt gttgatattc gggagtgtag tggttgagca cttcccgact ggagatatca
    25921 ataccgtgga agatggtggc aattttgccg cgaatgatac ccagttcgcg tagttttgct
    25981 gcggttaccc ccgccgggcc aaaatgagcg atgaacacat cggcgcgaaa cggtgttgcg
    26041 acctgaccac aaatggcaga caaaatcagg ttccgcgact cggcaccata gcgtttgagg
    26101 ttgagcgcat gccaggtatt tttacgatga atgccacgta aggtctggct ggcgcggtgg
    26161 cgcagtttcg ctactttgcc cgcaggttcg tcctgtaacc agcgggttct ggcgacaagg
    26221 ttgtatttcg tccatgccgc gtgggtgttc tctgtatcgc ctttttgcaa agcgacaatc
    26281 tccacttcaa agcccatatc aataaacgcg gtaatttgat tgaggacgaa ggtttccgac
    26341 gacagcggga atttcaataa gaagaagccg accttcattt cacctccccg atgcgctcaa
    26401 gcacagactg caccatctgc attcccgtct gacgttcgcg actgacggct tcgttaaatc
    26461 gagcgttcag caccggaagc tggcctaagg tatccgcaac catcgcttgc aggctgccgt
    26521 ccagtaaatg acgaatatca atcgccatct ctggcagtcc cagctgctgc ataatcccgg
    26581 cggatttatg ttcatagttg atggcaatcg ccggagtagc aaagttcatt gagataatgg
    26641 cggagtgcag gcgcgtaccg acggtgagtt cacaggctcc gagaattttg cccatttcca
    26701 gatcgttgag ttcatccatc actacgtggt aacgggcagg atcgctgatg tgctggcgca
    26761 gattgagcgc caccatgcgg tcgtctttgt tatagctgtc gatacccgta caggtggaga
    26821 gcgcaatcac ctgatagcct tcatcgagaa tgcgattgac caccccggca aaggcttttt
    26881 catacgcttg ttgagtggtg ccgagacgtt tgtcgaacgg tgccagttcg cgcagggtaa
    26941 ttgccactgt tttctgttgt gcggcaacgt ccagccagtg ttgaacggca tagctggcgg
    27001 tgaagtcttc ggtgtggtga tcgaccagcc acgcggtatc gacgccatgt tccacttttg
    27061 cggtggtgat atttctgcgt ttcatcaaat caaggctgac cgattcgcgc aggatcagcg
    27121 cgtcgcagtg accaaagaca tagttcgcca gttggttaaa ttgctcatcc tggaaggggc
    27181 cgacgctgtg accaatcata aacagcggct ttttcgccat aaatgtgcaa agcgcatgtt
    27241 caaactgcgg cacgccgtag agatcgacaa aaaacgatcc gccgacctga ataatggcgt
    27301 cgtaacctga cagcaggcgc acgaagtcgg tgaatccctg ggcgatggcg atattgcgca
    27361 gcttgccagt gtcggtgacg cgtgagagca atacctgatg ctggtagcgg cgacggagga
    27421 cttttttaat gcgcccgaca acgcccgccg cgctattgtg ttgtgtcatc tgcaggaaca
    27481 gcggatcgcc cattaccggg cggttgagca gccaggaaga actgaccgga tagcggctca
    27541 tcacgtccac ttcggtatgt ggattgagga tgttgatggc atcaagtaag ccgcgcaaga
    27601 tggcgctgtc gccacgattg ccgcaagtgt ggttgcccag aataagtaat ttcatttttt
    27661 cctcataaat ttgatgccag gtgaggctgt gtttgttgtg ttcgttgccg ttttttgcct
    27721 gatgcgacgc tggcgcgtct tatcaggcct acaggtcccg agcacagaac cgtaggacgg
    27781 ataaggcgtt ttacgccgca tccggcaacc gttgtcggaa ccgaaaacag caattcaccc
    27841 cgcccgtaaa agcattttca ttttttcgct gcgacaaaac tgacgcttca cttccaccac
    27901 cagcggatgg cgtgacagca caatcatcac cacaaacgcc agcacgcccg cggctatttg
    27961 caccgccagc agcatcccca gcgccagttg ccctttcagc acaatgccca gcgcataact
    28021 gaccaccagc gtcggcaacg agagataaaa cggcagccac aaactcagga tgtactggcg
    28081 ataactggaa ccaagaaccg gtttaatcat cacgaagtaa ctcagaatgg tgttgataat
    28141 ctgcaccagc aggaaaccga gcgtgacgcc gattgcgccc gccatctgcc caccaataac
    28201 aatcgccgga ataaacagaa atgttttgaa tacgttgaat ttaaagctga tatcgacccg
    28261 cgctttcgcc atcagcagcg aaccaatcgg gttacccacg gagcgcagca gacccacaat
    28321 acacagcaat tgcagcaccg gaataatgct gttccacttt tcgccgaata ccagcggtac
    28381 gaagttattc gacaccacca tcagccccag cagcgccgga aagttgataa tccccactat
    28441 cgacagcagc ttgtagaagt taacacgcag cttttcggtg tcgtcctgaa ttttggcaaa
    28501 cgccggaaac aacacgcggg tgatgattgg gttcagcttc atcggcggta cgacggccac
    28561 gttgtacgcc aggttgtatc cccctgccac gcccgcgcca agaatacgcg ccagcaccag
    28621 cgttgaaagg ttggtgttga gatagttgat gatgctgtcc gccgtcagcc aggcaccaaa
    28681 acgtaagttc ggtgccacag acgccagcga gaagtgcaga ccggggcaat aaattttgcg
    28741 tccaaagtag ccaaacagca gtgttctcac cgcactattg accagataac cgaggatcgc
    28801 ggtcatcgcc agcggccaga aatgggcgct aactaccgta aaagtgaagc ccgccagcac
    28861 cgcgctggtt tcgatcatgc cgattttgtt gaactccagc tccttctgca tcaacgcgcg
    28921 gaactgttgc ccgtggggga tcaccacaaa cgccagcgac aatgttttaa tcaacggtgc
    28981 caggtccggg ttattcagca cgtcgccgat gacatcactc aacaaaaaca ccgccacaca
    29041 taccacaagc cccagcccga cgttcagcca gtacagcgtg gtgagttcaa ggtgactgat
    29101 ttcttttcgc tgaataatcg agttagcgat accgaagtca gaaagcgtat ctgccagcgc
    29161 gataatcacc agcgacacag tcagcaggcc gaactggtgg ttgtcgataa tccgcgccag
    29221 cacggtcatc tgcaccagcc cgaggccgat gatgatcacc gtggcaatag ccgaccactt
    29281 cgcgccgctg atggtttttt cacgtaagct catatcaata tgccgctttg ttaacgaagc
    29341 ccttgaatac cgtcaggaaa acgattttga tatcgaacca gacgctccat tcgcggatgt
    29401 actcaaggtc gaactcgacg cgtttttcca ttttctccag cgtgtcggtt tcgccgcgcc
    29461 agccattaat ctgcgcccag ccggtaatgc ccggtttcac tttatggcgc agcatgtagc
    29521 cttcaatgag ctggcgatac tgttcgttat gcgctactgc gtgcggacgt ggaccgacaa
    29581 tcgacatccc cccggtcagc acattgataa actgcggcaa ttcgtccagc gaggtgcggc
    29641 gcagaaagtt ccccacttta gtgacgcgcg gatcgttctg cgtcgcctgg gtcaccactt
    29701 tgtcgttctc catcactttc atggaacgga acttccacac tttgatcggc ttgccatcca
    29761 taccatagcg agtctggcgg aaaataaccg gccctggtga actgagtttc accgccagcg
    29821 caatacagca cagcaccggg gagatcagca gcagaataag cgtcgccagc acaatgtctt
    29881 ccgcacgttt tagcaggcgg ttaacccccg aaagcggcgt gtcatacagc ggcaccaccg
    29941 gaacgccgtt catctcttcg agacgtgaat ggagaatgtt gaaggtaaag acgtcgggga
    30001 tcagcagcac cgaacaggtg gtatccgcca gttgatggac cagttttttc actcgtgcgc
    30061 cgtcgcacat ttgcatcgcg atatagacgt tatgaatctt gcccgctttt gcatcctcga
    30121 ccagctgttg caggttaccc gcccagtcgt tagaaacgcc gcccggtttc gggtcgtggt
    30181 aaacgcccac cacttcaaac cctaaccacg gctgattacg gaaactctcc atcagcattt
    30241 gtccggcggc taaatccccc gctacggcga ccatccgctt gttgtagcca tgattacgca
    30301 gccagcccgc cccaatgcga atacatgaac ggcaaaccac cagtccgatg ctggtcagcc
    30361 cataccacgc cagccagatt ttcagttgcg tgtcgaaatc attgttgaac gccaccagtc
    30421 cggcgctgaa aatcacgctt aaggtccagt tttgtagcaa cagggcaaat tctgtcgctg
    30481 cccgaacacc gcgccatgag cgataaaaat cggtgatgcc gcccagcatc tggaacacca
    30541 ccagcgtaat cagcgccacc aacaggtgca tgtagaggaa tgacagtccg ctgacttcgc
    30601 aaaccagcca tagcccggca aacatgatgg tgatatctga aaagcgttgc accatagaga
    30661 ttaacgatgc attggttttc gctcgctcgc gcttttttag atttgtcatc gttgttcctg
    30721 ttattagccc cttacccgca gtggggtaag ggaagatccg acattactcg ttcagcaacg
    30781 tcagcagagt tcgcgttcgc gcttccatca gcggcacatc accgcgcgat tccacattca
    30841 ggcgcaccac cggttcggta ttggaggtgc gcaggttaaa gcaccagtcg gcaaaggtca
    30901 tgctgatgcc atcgctgcga tccaccgcca gcgcctcacg gctaaaatgc tgttccacgc
    30961 ggttaatcgc ctcaacgggt tgcgccagtt tgctgttgat ctcaccgctt gccggaaacg
    31021 ccgccatccg gtcgcgcacc agttcgccca gcgtttttcc tttcaggcac accagttcgg
    31081 cgaccagcag ccacgggatc atgccgctgt cgcagtaagc gaaatcacgg aagtaatggt
    31141 gggcgctcat ttcgccaccg tagatggcgt cttccttgcg catccgttct ttaataaagg
    31201 cgtgtccggt tttcgacatt accggcgtgc cgcctgcagc agtcaccaca tcaatagtgt
    31261 tccaagagag acgcggatca tggatgatct tcgcgccggg atttttttcg aggaacgctt
    31321 ctgccagcag gccgacaatg tagtagccct cgataaactg ccctttttcg tcaaacagaa
    31381 aacagcggtc aaaatcgcca tcaaaggcaa tgcccatatc cgcgccgtgt ttgatgaccg
    31441 cattgcgggt gtcgtcgcga cattccggca gcagcgggtt aggaataccg ttggggaaat
    31501 tgccgtccgg tgtgttgtgt actttgatta attccacggg cgcgccgagg gctttaaagc
    31561 gggcttcaat ggcgtccacc accggacccg ctgcgccatt cccggagttg atcaccagct
    31621 tgagcggcgt gagatttttg acattgatat aaccgaacag gtgatcaacg taagcgtcac
    31681 gcaggttgat ttgctgatag cgaccgcgtt tggtttcatc gacgggagga aagtcgttgg
    31741 cttcagccag acgctggacg tcgcgcagtc cggtatcacc gctgatcgtg cgagccccct
    31801 cgcgcaccag cttcatgccg ttgtaatcca tcggattatg gctggcagta acttcaatgc
    31861 cgccatccac gccgagatgg aacgtggcga aatagatctc ttcggtgccg gacataccga
    31921 tatccagcac atcgacgccc gcatcctgta aaccttttgc cagcgccagt tttaaggttt
    31981 cgctggtgag acggacatca ccgcctaaca caatggtttt cggtttgaga aattcgccat
    32041 aggcgcgacc aatgcgccag gcgatatctt cattcagttc ttcgcctaat ttcccgcgaa
    32101 tatcataggc tttaaagcag gttaattttt tcatgtcgtt accctttttc aggcaatagt
    32161 tggccctgac cgaaacggcc aattttattt ttgtcgttat ttacaccgcg gtttcgcatt
    32221 cattgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaaccgaac cgtaggtcgg
    32281 ataaggcgtt cacgccgcat ccgacaaaca gcgcctgatg cgacgtttac acccgtccgt
    32341 agcgatccgc gaagcgcacc acatcatcct cttcgagata agagccggag cgcacttcaa
    32401 ttaaatcgag cggaattttc cccgggtttt ccaggcagtg cgtcgccccc agcggaatat
    32461 aaatggactc gttttcacca agcagtttga tatcaccatc aatggtgact tttgccgttc
    32521 ccgcgaccac tacccagtgt tccgcgcggt gatggtgcat ctgtaccgac aaaccctcgc
    32581 ccggtttcac ggtgatgcgt ttcacctggt agcggtcgcc cgcgtcgata gagtcatatt
    32641 tgccccacgg acgatacact tcgcgatgca cccgatgctc atggcgacca tcggctttga
    32701 tctgctcgac cacttttttc acatcctgta ccgcgttacg gtcggcaatc agcaccgcat
    32761 ctttggtctg caccactacc agatctttca ccccgacggt ggtgaccagg cctgattcgg
    32821 catacacata gctgttttca gttttgtgat taatcacatc gccgtggcaa acgttgccct
    32881 cggcggtgtg ggcgctgatc tcccataatg aagaccagga accaacatcg ctccagcccg
    32941 catccatcgg caccacaaca gcatctgccg taggttccat gaccgcgtaa tccaccgact
    33001 cttccgggca ggcgagaaac gcttcttcat ccacacgaat aaaatcgaga tccggatcga
    33061 cggcgctcat cgctttttcg caggcatcga ggatatccgg gcgatatttt ttcagttctt
    33121 cgagatagcg tccggcgcgg aacaggaaca tgccgctgtt ccagtaatat tcgccgcttg
    33181 ctacataggc ctgggcggtt tccagattcg gtttttcgac aaactgcgcc acttcaaagg
    33241 ccaccgtatc ctgctcaccc acagacactt caccgcgacg aatatagcca taaccggttt
    33301 ctggtagatc cggcacaatg ccaaaggtca ccagcttgcc cgcttcggca taaggcatgg
    33361 cattacgcac ggcggcacgg aacgcgtctt catcggcaat cacatgatcc gccgccaata
    33421 ccagcattaa cgggtcgctc tccgggctat gacgttttgc cgccagcgcc gccagtgcaa
    33481 tggcaggtgc agtgttacgc cctgccggtt cgagaataat gttctcggtg agtttgttca
    33541 gttgacgcag ctgttccgcg acaataaagc ggtgctgctc attgcaaatc accaccgggc
    33601 tttcgcactc cacgccgttc aggcggcaga tggtggtttg cagcatggtg agatcgcctt
    33661 tcaggcataa aaactgcttg gggtaaagta cgcgggaaag cggccataaa cggctaccgg
    33721 agccacctgc catcacaact ggatagagtt tcgactgcgc cataattatc cccgaatatc
    33781 atttataaat tgacgtaaca cgttctcttt atcgagcgtg cgttcggcat attcacgtgc
    33841 caccgtgtta tgtttgggca gcaggagcgc ctgacgaatc cccgccacca gcgcctcgac
    33901 cgattccggt tctacgcaaa ccgcaatacc cggaaaggtt tcgcaaagct gccccagttc
    33961 tgtgtgggcc tctgcggtaa tcaccgcgtt accgcctacc gccagaatat tggtcagttt
    34021 cgacggcaat acggcatctg ccgcgccgcg tttttgcacc accagatggc aatcgcccat
    34081 cttcagcagt gcaggtaaag cgtcatacga ttgcagcggg aaaaattgca tgttgcgcag
    34141 tccacgctgc tgcgccattt tttccagccg cgctttgccg ccgccctgcc cgacaatggc
    34201 aaaaatcagc ggttcatcgc gcaggagatc ggcagcttca ataacgtttt ccagcccctg
    34261 cttttcacca atattgccgg agtaaagaat gatttttttg ttatccggca ggccaagctg
    34321 gttacgaagg gcatcaacat cggcgtctgc aacatgctga aaacgggcaa tttccgacca
    34381 gttggggaag aagatgacgt tatccgccgc cacgcctttt tcgatggctt tattcatcat
    34441 cgaacgcgaa atcgtggaaa cgttatcgac gttatgcagt ccgctacgtt cgaacgccgt
    34501 taccagctgt gccactttgc cgcctttgcc ttttccggca aggcccagcc ccagcatggc
    34561 gtccacttcg taatcctgaa tatgcagcac ggtacgcgca ccagagagct tcgccagcag
    34621 gcgcattccc ggcgtgcaaa acagcgttgg cactacgcca ataatgcgat ccggcttcca
    34681 gcgacgttgc gccatcagtg gaaagaaact gctgacggca aaactgccga gatgcaacaa
    34741 gcgtttcagg gtgctcggct gttttggcac gtacagcggg cagcgccaca ccgtggcagc
    34801 cccttcttct cgtttgtagc gccaggcgga atagttctca cccacctgcc attgcgggta
    34861 gtaaggcggt gcggtaatga cccgcacctc atgaccttgt gccgccaacc attccaccat
    34921 ctcgccggtg tatttgccga tgccggttaa ctccggcgag tagttaatgc cgtagaccag
    34981 tattttcata atccgggtac tccggcacgc ttctcagcga gaaaataggc tcggctgtta
    35041 gcgtgaacat cgttactggc gagcagcgcg tccggcgtca gccagcggta atcgtcatgc
    35101 tgctcatccg gcagtaacag ctcttcttcc gctactctga agcgaaaacc gagcaccaca
    35161 tagtgagtgg tgaaatccgt gccggagaag ttatcgtcat aaaaatgctg ccagacaccg
    35221 taaaactggc ctgctgttat cggcagacgc agccccagtt ccgccatcgt cagccgctca
    35281 aatgcggctt ccagcgtttc gtctttctgc acgcgccctc ccggcacaaa ccagtaaccc
    35341 tgcgccgggc ggttggttct tttgccaagc agaaactcgc cgcgactgtt ctcgacaata
    35401 aagtcgagag agacaagcgg tgtggagcgc actacagtgg caaagtcttc ctgacgtaaa
    35461 aacatcattc cccccgaaag cggtcttgat tctcaaggaa ccactggtaa gtgctggcaa
    35521 gccccgcttc cagtgagatt tcgtgatacc agccaagcta atgcaggcgc gtcacatcca
    35581 gcagtttgcg cggcgtacca tccggtttgc tggcatcaaa aaccacccga cctttgtaac
    35641 ccaccacttt ggcgatggtt tgcgccagct cgcggatagt gcagtcaacg cccgtgccga
    35701 cgttaatgtg cgacagcatc ggttgggtat tctccagcca gacttcatgc gccagctcca
    35761 tgacatgaat gctcgccgcc gccatatcat cgacgtgcag gaattcacgc atcggtgtac
    35821 cgctgcccca taccaccacg tccggcgcat tctgtgccgt cgcctcgtgg aagcgacgca
    35881 gcaatgctgg tatcacatgg gagttactcg ggtggaagtt gtcatgcggc ccgtacaggt
    35941 tggtcggcat gacagtgcgg taatcgcgtc catactggcg gttgtaagat tcgcacagtt
    36001 tgatcccggc gattttggca atggcgtaag gctcgttggt cggctccagc gtgccctgca
    36061 ataactcgct ttctgccatc ggctgttttg ccagtttcgg gtagatgcag gacgatccga
    36121 gaaacatcag tttgttcacg tcgttctgat gcgcggcgtg aatgatgttg ctctcaatca
    36181 tcatgttctg gtagatgaaa tccgccggat aggtgttgtt ggcaacaatg ccgcccactt
    36241 tcgccgccgc cagatagacc tggtcaatgc gttcgctggc aaagaaatca tgcacggcgc
    36301 ggctgtccag caggttcagt tcatcgcggg tacgtaatac cagctccaca tcaccgcgct
    36361 gttcgagctg ccgcttgatg gcagaaccga ccatcccaca atggccagcg ataaaaatgc
    36421 gttgtttact catgcttatg actccagcgc gatcgccacg tcgtagccgt gagatttcag
    36481 cagagagtgt tttttcgccg cttcgaggtc attagccacc atttcagaca ccatctctct
    36541 gagggtgatt tccggtttcc agcccagttt ttcgtgcgct ttggtcgggt cgccgagcag
    36601 cgtttcaact tctgccggac ggaagtaacg cggatcaacg gcgataatca catcacccgg
    36661 tttaacgccc ggcgcgtcat gcccggtgac ggaaaccaca atgcccttct cttcaacacc
    36721 cgtgccttca aagcgcagtt tgatgcccag ctgtgctgcc gccatttcca caaactgacg
    36781 tacggagtac tgaacgccgg tcgcgataac gaaatcttcc ggctgttcct gttgcagcat
    36841 catccactgc atttttacgt agtctttggc gtggccccag tcacgcaggg aatccatatt
    36901 gccgaggtac aggcacgact ccagcccctg ggcgatgttg gcgattgcgc gggtgatttt
    36961 gcgggtaacg aaggtttcgc cgcggcgcgg ggattcatgg ttgaagagaa ttccgttaca
    37021 agcgtacatg ccgtaggatt cgcggtagtt aacggtgatc cagtaggcgt acagtttggc
    37081 gaccgcatac ggagatcgcg ggtagaacgg cgtggtttct ttctgcggaa tttcctgcac
    37141 cagaccatac agttcagagg tggaagcctg atagaaacga gttttctttt ccagaccgag
    37201 gaagcggatc gcctccagca ggcgcagcgt acccatcgcg tcgacgtcag cggtatattc
    37261 tggtgactca aaagagaccg caacgtggct cattgcgcct aggttgtaca cttcatccgg
    37321 ctgtacttca cgcaaaatgc gcgtcaggtt agaggtatca ctcaggtcgc cataatgcag
    37381 atggaatttc gggttgcagg tgtgcggatc ctgataaatg tgatccacgc gctcggtgtt
    37441 gaatgacgat gcgcgacgct taataccatg cacctcgtaa cctttttcca gcagaaactc
    37501 tgccaggtaa gaaccgtctt gtccggttac accggtgatg agagcgactt ttgacatgtc
    37561 ttattcctct gtatttttga atttattcag tttcaacgcg ttcgcgtatc accactgcgg
    37621 ggttcccccg gcaaaccaca tttgccggaa gcgatttaaa aacactgctt cgtgcaccca
    37681 cgacggtgcc gtcgccgatt gtgacgcctg gggcaacaaa gacatcggtt gccagccagc
    37741 atttctcgcc aatcacaata ggcgtggcgt taatggtgaa atgttgactt gcatggtcgt
    37801 ggctaccggt gcataaataa cttttttgcg atatcaccga atgtgcgc
//
LOCUS       DAJVPP010000030        37062 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVPP010000030 DAJVPP010000000
VERSION     DAJVPP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.030"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(112..996)
                     /gene="yhdJ"
                     /locus_tag="OQM34_001504"
     CDS             complement(112..996)
                     /gene="yhdJ"
                     /locus_tag="OQM34_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1452002.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1082..1378)
                     /gene="fis"
                     /locus_tag="OQM34_001505"
     CDS             complement(1082..1378)
                     /gene="fis"
                     /locus_tag="OQM34_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1452003.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1404..2369)
                     /gene="dusB"
                     /locus_tag="OQM34_001506"
     CDS             complement(1404..2369)
                     /gene="dusB"
                     /locus_tag="OQM34_001506"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1452004.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2698..3579)
                     /gene="prmA"
                     /locus_tag="OQM34_001507"
     CDS             complement(2698..3579)
                     /gene="prmA"
                     /locus_tag="OQM34_001507"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1452005.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3591..5042)
                     /gene="panF"
                     /locus_tag="OQM34_001508"
     CDS             complement(3591..5042)
                     /gene="panF"
                     /locus_tag="OQM34_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1452006.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5032..5274)
                     /locus_tag="OQM34_001509"
     CDS             complement(5032..5274)
                     /locus_tag="OQM34_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1452007.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5383..6732)
                     /gene="accC"
                     /locus_tag="OQM34_001510"
     CDS             complement(5383..6732)
                     /gene="accC"
                     /locus_tag="OQM34_001510"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1452008.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6743..7213)
                     /gene="accB"
                     /locus_tag="OQM34_001511"
                     /gene_synonym="fabE"
     CDS             complement(6743..7213)
                     /gene="accB"
                     /locus_tag="OQM34_001511"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1452009.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8191..9165)
                     /locus_tag="OQM34_001512"
     CDS             complement(8191..9165)
                     /locus_tag="OQM34_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1452010.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9317..11257
                     /gene="csrD"
                     /locus_tag="OQM34_001513"
     CDS             9317..11257
                     /gene="csrD"
                     /locus_tag="OQM34_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1452011.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11562..12605
                     /gene="mreB"
                     /locus_tag="OQM34_001514"
     CDS             11562..12605
                     /gene="mreB"
                     /locus_tag="OQM34_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1452012.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12671..13774
                     /gene="mreC"
                     /locus_tag="OQM34_001515"
     CDS             12671..13774
                     /gene="mreC"
                     /locus_tag="OQM34_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1452013.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13774..14262
                     /gene="mreD"
                     /locus_tag="OQM34_001516"
     CDS             13774..14262
                     /gene="mreD"
                     /locus_tag="OQM34_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1452014.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14271..14864
                     /gene="yhdE"
                     /locus_tag="OQM34_001517"
     CDS             14271..14864
                     /gene="yhdE"
                     /locus_tag="OQM34_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1452015.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14854..16323
                     /gene="rng"
                     /locus_tag="OQM34_001518"
     CDS             14854..16323
                     /gene="rng"
                     /locus_tag="OQM34_001518"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1452016.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16391..20191
                     /gene="yhdP"
                     /locus_tag="OQM34_001519"
     CDS             16391..20191
                     /gene="yhdP"
                     /locus_tag="OQM34_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1452017.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKELVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20347..21792
                     /gene="tldD"
                     /locus_tag="OQM34_001520"
     CDS             20347..21792
                     /gene="tldD"
                     /locus_tag="OQM34_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1452018.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21920..22849)
                     /gene="aaeR"
                     /locus_tag="OQM34_001521"
     CDS             complement(21920..22849)
                     /gene="aaeR"
                     /locus_tag="OQM34_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCS1452019.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23032..23235
                     /locus_tag="OQM34_001522"
     CDS             23032..23235
                     /locus_tag="OQM34_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1452020.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23243..24175
                     /gene="aaeA"
                     /locus_tag="OQM34_001523"
     CDS             23243..24175
                     /gene="aaeA"
                     /locus_tag="OQM34_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS1452021.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVHPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24181..26148
                     /gene="aaeB"
                     /locus_tag="OQM34_001524"
     CDS             24181..26148
                     /gene="aaeB"
                     /locus_tag="OQM34_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1452022.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26240..26512
                     /locus_tag="OQM34_001525"
     CDS             26240..26512
                     /locus_tag="OQM34_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1452023.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26568..26831)
                     /gene="yhcN"
                     /locus_tag="OQM34_001526"
     CDS             complement(26568..26831)
                     /gene="yhcN"
                     /locus_tag="OQM34_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1452024.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27196..27666)
                     /gene="argR"
                     /locus_tag="OQM34_001527"
     CDS             complement(27196..27666)
                     /gene="argR"
                     /locus_tag="OQM34_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1452025.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28101..29039
                     /gene="mdh"
                     /locus_tag="OQM34_001528"
     CDS             28101..29039
                     /gene="mdh"
                     /locus_tag="OQM34_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1452026.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29101..30168)
                     /gene="degS"
                     /locus_tag="OQM34_001529"
     CDS             complement(29101..30168)
                     /gene="degS"
                     /locus_tag="OQM34_001529"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1452027.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30258..31605)
                     /gene="degQ"
                     /locus_tag="OQM34_001530"
                     /pseudo
     CDS             complement(30258..31605)
                     /gene="degQ"
                     /locus_tag="OQM34_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31759..32157)
                     /locus_tag="OQM34_001531"
     CDS             complement(31759..32157)
                     /locus_tag="OQM34_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1452028.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32351..33478
                     /gene="zapE"
                     /locus_tag="OQM34_001532"
     CDS             32351..33478
                     /gene="zapE"
                     /locus_tag="OQM34_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1452029.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQNDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33697..34125
                     /gene="rplM"
                     /locus_tag="OQM34_001533"
     CDS             33697..34125
                     /gene="rplM"
                     /locus_tag="OQM34_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1452030.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34141..34533
                     /gene="rpsI"
                     /locus_tag="OQM34_001534"
     CDS             34141..34533
                     /gene="rpsI"
                     /locus_tag="OQM34_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1452031.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34928..35566
                     /gene="sspA"
                     /locus_tag="OQM34_001535"
     CDS             34928..35566
                     /gene="sspA"
                     /locus_tag="OQM34_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1452032.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35572..36069
                     /gene="sspB"
                     /locus_tag="OQM34_001536"
     CDS             35572..36069
                     /gene="sspB"
                     /locus_tag="OQM34_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1452033.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36112..>37062)
                     /gene="dcuC"
                     /locus_tag="OQM34_001537"
     CDS             complement(36112..>37062)
                     /gene="dcuC"
                     /locus_tag="OQM34_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1452034.1"
                     /translation="LLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAAQ
                     VAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPP
                     LYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHNLRETM
                     DDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIM
                     ALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITA
                     VVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8950 a         9273 c         9773 g         9066 t
ORIGIN      
        1 cgacgagcca ccaatacttg cgaatcatta cctgagcctg tgcatgttta accaagtctg
       61 gcaggaaata tacacgaaat gaataatctg aaaactaggc ttacacttcc tttactttgc
      121 aatgagatcg gggtcaactt cgcttaaccg gcttcgtttt gacaggttgc cggtctttct
      181 ttttttcact ttcgccagtt cttccgcgga gtaatgcgac gcgacatcca gccgtcgaag
      241 ccccattttg atgtactcgc tgttgatctc aataccaatg aattttcgtc cgctggcgat
      301 ggctacggca ccggtagtaa agctaccggc aaacgggtcg agaacgatat cgcctgggtt
      361 ggaagaggcg agaataatgc gtttcagtaa ggctttcggt ttttgcgtcg ggtggttctc
      421 atattcatcc atcaaataac gcacgcgcgg aaaatcccag acgttacccg gtactttttg
      481 atgattgtat ggctgtggag gatttttgcg ataatcgatc aacgcgcgct gcgatccggt
      541 tttggcttct accagaatag catcaccgtt gaatgtgtag ttctttgcgt ctttcaccat
      601 catcaggatg ggttcgtaca tggagccgta gtgttttttc gcctgtactc cagaactgtc
      661 atatgaccag acgatgcgac ttttgatggt aaaaagcttg cggcactgga gatcgataaa
      721 gggcatgttt tccgtactgt tcataatgta catgctgccc tgctttttca gaacgcggtg
      781 gcactctgca atcacttcaa acagccagtc gataaacaga tcttctttcc aggcttcgat
      841 cagaccatca aaatttttac cgatgttata cggtgggtcg gcaaagagca gatcgacgct
      901 ttcagtaggt agctttttaa gttcggcaaa ggcatcaccg tgaataatgg tcttagcttc
      961 attaccaaac cgggtcggtt cacatcctgt tctcatggtc actccctttg tgacacctat
     1021 aaaaaaggcg cttccccatg ccgagtagcg cctttttaat caagcattta gctaacctga
     1081 attagttcat gccgtatttt ttcaattttt tacgcagcgt accacggttg atgcccatca
     1141 tcagcgcagc acgggtctgg ttaccacggg tgtattgcat caccatgtcc aacaggggct
     1201 gttctacttc agccagtacc agctcataga ggtcattcac atcctgacca ttcagttgag
     1261 caaaatagtt cttcagtgcc tgtttaaccg agtcacgcag gggtttttgg gttacctgat
     1321 cctgagagtt aacggtagaa acggtcagta cgtcagaatt tacgcgttgt tcgaacatag
     1381 ttctgtcagc tctttatttc tgtttacgca aaattttcga agtatgcctc caacgcctcc
     1441 agctgttcgc tggcatcctc aatggcgttg aatgtgcgcc gaaactggtc atttggagcg
     1501 tgttcctgga gataccagga aacgtgttta cgtgcaattc ggtacccttt tgccggacca
     1561 taaaagtcat gcagttcccg aacgtgcgcg caaagcaagc gcttaacctc tgccaaaggc
     1621 agtgggggca gcaactcccc agtgtccaga taatgctgga tttcccgaaa gatccagggt
     1681 cttccctgag ctgcgcggcc tatcatcagg gcatccgccc ctgtatagtc gagcacagct
     1741 ctggctttaa gcgggtcagt aatgtcgcca ttcgcgataa ccggaatgga aactttctgc
     1801 ttaactgccc gaatactgtc gtactcagct tctccattga acaaacaggc gcgtgtacgg
     1861 ccatgaatgg tcagagcctg aatgccacag tcttcagcca gttgggcaat ctcttcgcag
     1921 ttacggtgtt ccggtgccca gccggtgcga atcttcaggg taacaggaac gtccactgca
     1981 ttgacgacct cggtaaggat cgatttaacg acatccgggt actgcaagag ggctgaacct
     2041 gcgagcttgc gattcacttt tttagccggg caacccatat tgatatcaat aatctgggca
     2101 ccgctttcca cgttaatacg tgctgcatct gccatttctt tcggagcgct accagcaatt
     2161 tgcacggtgc gaataccggg ttcatcaatg tgcaccatcc gtaaacgaga tttgtcgctt
     2221 tcccaaacct gtgggttaga agacatcatc tcggatactg tcaatccggc tcccatctcg
     2281 tagcacaacg tccgaaaagg tctgtctgta atgccagcca tgggcgctgc gatcaggcga
     2341 tttctgagct gatattgtcc gatgcgcatg agttaagaaa tgaccatact gtgactgcaa
     2401 ggcggcgtat attacgcatt ttttgcacga gatgaaaggc caaactttga ccaatcctct
     2461 gagatggatc aaagaattgc atttaaaatg agcgtggtgc gataattact cataaaaatc
     2521 atcatattag aaaatagtga ctaaaattta cactcaaaga aatttgagta agttctcaat
     2581 ttttctttat gaatgaaaat tttggcacgc aaatttgcgt aaataatcgg caaatttacg
     2641 tgccttttgt gagcttgctc gcacttcgcc ccgcgtcacc ctacggcgat gcgaaggtca
     2701 attcttacga ccggtaatac ggcaccactc ttctttttcc acgaccgggt ccagtgcgaa
     2761 gctatcggca taagcttcac aaacgctctc tgcctggctt gccagaatac cggaaaggcc
     2821 cagcaaaccg cctgaaaccg gcaggacgct gattaacggt gccagttcac gtaatgggcc
     2881 tgcaaggatg ttagcgacca ccacgtcggc tttcatttct tctggctgat ctttcggtaa
     2941 gtagagttcc agacggtcag aaacgccatt acgttcggcg ttatcgcggc tggcctgaat
     3001 cgcctgcgga tcgatatcaa taccaatggc ttttgctgca cccagtttca gcgccgcgat
     3061 cgccagaatg ccggaaccac agccaaagtc gatgactgtt ttaccggtta aatcgaggct
     3121 gtcgagccat tgcaggcaca gagaggtggt tggatgggta cccgtaccaa acgccagccc
     3181 tggatctaac atcacgttga cggcgttttc gtccggcaca tcacgccagc tagggcagat
     3241 ccacagtcgt tcaccaaagc gcatcgggtg gaaattatcc atccattcgc gctcccagtc
     3301 tttatcttct agttgttcga ttttatgcgc gaagcctgcg ccgagcagcg gatggttttc
     3361 cagaatcgcc accacgtcgt tcatatcggt ttcagcgtcg aacagaccaa tcacatcggt
     3421 gtcgccccac aggcgcgttt cgcccggcag cggctcaaat actggcgtat cgtgggtatc
     3481 ctgaaaagtg atagaaacgg cacccgcttc catcagcgca tcgctaagat cttccgcgtt
     3541 cgcgccggtg gtgttcagtt tcagttggat ccaaggcatg gcaaaactct ttatttatca
     3601 gtagtcaaaa cggtagcttg cgggacggat gtaccgaaac ggtttccgac caggaaagcc
     3661 agcaaactta gtagtaacga gggcacgata gggtggaagc ccaggtactg aatattcagc
     3721 gtcgcgagta cggcatacag cacgccgcca acgatcatcg cacttagcgc gcctttggcg
     3781 ttggcgcgtt cccagtaaag acccagcacc agcggccaca ggaaaacggc ttccagccca
     3841 ccgaaggcca gcaaattcag ccagatgatc atttctggcg gcttccaggc ggcaagcagc
     3901 agcaacgcgc cgagaactaa cgtaattacc gccgacatcc gcttcagacg cgtctcgttt
     3961 tgcatttgat ccggacggat attcagatag agatctttaa tgatcgtagc ggaactttgc
     4021 agcagttggg cgttaattgt cgacatgatc gcagccatag gtgcagccag aaagatcccg
     4081 gcagcaaaag gtggcagcac ttttaccatt aacgtcggga tcaccagatc cggtacggtg
     4141 agatcgggga tcactgcacg acctaacgct ccggccaggt gcataccgaa catcaggatt
     4201 gcgactataa tcgtaccgat gatgatcccc cggtgtactg ctttgctgtc tttataagag
     4261 atacagcgca ccgcagtatg cggcaggcca atcacgccaa agcacaccag tacccagaac
     4321 gacgtcataa aggcaggcga cagaatatcg tcagcgcctt gtggcgtaac cagttgcgga
     4381 tcgatggttt gcaaggtctg tactgcgttg cttaagccgc cagcggcatg taccacgcca
     4441 ataagcagca caacggtgcc aatcagcatc acaagccctt gcatggtgtc gttcagcacg
     4501 ctggcgcgaa agccaccaaa ggcggtatat aacgcaatgc tgataccaaa aatcagcagc
     4561 ccggtttcat aaggaatacc cgccgcggtt tccagcaggc gcgcaccgcc gataaactgc
     4621 acggtcatcg caccaacgaa cgcaaccagc aaactcaaac tcgccagcca caccagaaga
     4681 cgactctggt agcgggcaaa cagcatatcg ttcagcgtca ctgcattgta gcggcgcgca
     4741 agaatcgcaa acttcttgcc gagaataccg agtgaaagcc agacagcagg aagctgaatc
     4801 atcgccagca atacccagcc cagcccgtat ttataagcag ctcctggccc gccgataaac
     4861 gaactggcac tgatataggt cgcggtgagc gtcatcgcca gcacaatacc gcccatagag
     4921 cggctgccga ggaaatactc attaaggaag gtgccggtgc tccgtttacg catcgcataa
     4981 accgagatac cgaacaccac caccagatag gcgaccagcg gtagaattac ttcaagctgc
     5041 atcgtcatcc tccagtggga tatcgcgata gataaatttc accatcgccc agcacagtcc
     5101 aataaacagt agcggcgtca ggatgcaggc catctcaaac cagcgcggaa agccggtaaa
     5161 accgggggca acgccaggta agtaagcgtc tactaaccaa actgccagat acaaaagggt
     5221 cagccccagc gcccagcgcg cctctttatg ggcctgaaca aaacgagtgt ccattttttg
     5281 tccctgatgg gtgaagaaag cgaggattgt accttatggg ggttgtcgat ccccagtaat
     5341 aaaaaaggcc ggaaaatccg gccttttgac gcttttgcag tcttattttt cctgaagacc
     5401 gagttttttc tccagatagt ggatgttagt gccaccatgc tggaagttct cgtcattcat
     5461 gatgcggatc tgcagatcaa cgttggtttt gataccgtcg atgatcagct cctgcagcgc
     5521 attcttcatg cgggcaatcg ccacgtcacg gttttcaccg tagcaaatca gcttaccgat
     5581 cattgagtca tagtacggcg gtacggtgta gcccgcgtag atatgagact cccaacgtac
     5641 gccaaaaccg ccaggtgcgt ggaaacgggt gattttgcct gggcttggca ggaaggtgtt
     5701 cggatcttcg gcgttgatac gacattccac cgcatggccg cgaacgtgaa cttcttcttg
     5761 cttgatcgac agcggttgac cggcagcgat acgcagctgt tctttgatca ggtcaacgcc
     5821 ggtgatcatt tctgtaaccg ggtgttctac ctgaatacgg gtgttcattt cgatgaaata
     5881 gaactcgccg ttttcgaaca ggaactcgaa agtacctgca ccgcgatagc cgatatcaac
     5941 acacgcttta gcgcaacgtt cgccgatgta gcgacgcagt tccggggtaa tgcccggtgc
     6001 tggtgcttct tcgaccactt tctggtggcg gcgctgcatg gagcagtcac gttccgccag
     6061 atagatagcg ttgccctgac cgtcagccag tacctgaatc tcgacgtggc gaggattctc
     6121 caggtatttc tccatgtaaa ccatatcgtt gctgaaagca gctttcgctt ccgcacgggt
     6181 catggagatg gattgtgcca gttcagcgtc gccgcgcact acgcgcatac cgcgaccgcc
     6241 gccgccgccg gaggctttga taatcaccgg ataaccaatg cgtttagcaa tggcgcggtt
     6301 tttatccata tcgtcgccca gcgggccgtc agaaccaggt acgcaaggaa cgcccgcttt
     6361 tttcatcgcc gcgattgcgg atactttgtc gcccatcagg cgaatggttt ctgctttcgg
     6421 gccgatgaag ataaagccgg agcgttcaac ctgctcggca aagttggcgt tctcggagag
     6481 gaagccgtaa cccggatgga ttgctactgc gccggtgatt tcagcggcgc tgatgattgc
     6541 cgggatgttc agataacttt ttactgacgg agcagggcca atacagaccg tttcatctgc
     6601 cagtaatacg tgttttagat cgcgatccgt gctggagtgc acagcgacag tcttgatgcc
     6661 cagttcttta caggcacgaa gaatacgcaa tgcaatctcg ccgcggttgg caataacaat
     6721 tttatccagc atgttcgcct cgttactcga tgacgaccag cggctcgtca aattctaccg
     6781 gttgtccact ttcgaccaga attgctttca cggtaccgga tttgtccgct tcgatctggt
     6841 tcatcatttt catggcttca acgatgcaca gggtatcgcc cacgttgact ttctgaccca
     6901 cttcgatgaa cgcttttgcg tccgggcttg gggtgcggta gaaagtacca accatcgggg
     6961 aacgtacgat gtgaccactg atttccgctg ctgctggcgc ttccatggaa ggaacggtcg
     7021 ccggagcggc tgcgttagat tgagctggct gctgcatcat tggtgcagcg taagcttgtt
     7081 gcatcacagg gaaacttgcg gcaggagctg cacggctaat gcgtactgac tcttcgcctt
     7141 cagaaatttc cagttcggag atgcctgatt cttcaaccag ctcgatcagt tttttaatct
     7201 tacgaatatc catgagtggg ttccgtactc tttgtttagt gtgattgtga caggcgtttc
     7261 accgccgtct gtaaagcgta tgccccgaca gctgtatgca tagcgataaa ttccagcagg
     7321 ccgggacgcc ggtctatttt gccccagagt gtcaatgtta gacttgacgg acattgtgca
     7381 gcgatctgct gctatcctcc ggcaaaaaac aaaatatacc ttcgttgcgc caatgtcacc
     7441 ttttccgcag cgcaaaaact gcgtcaggga ggacgaggtc gcacattata acgatttcgt
     7501 agcaattggc agctaaatac tggtcttatc agggaatata atcaacagct aacatgtaaa
     7561 taaccttcaa caccgtgtaa tttgcaacaa gccgcacaat tcacgaaatt agcgccatca
     7621 tcgacggaac ttcttataac gtaaggtgaa aagctgaaaa gccagccctg cgaagatgag
     7681 gggctgcaat gagataatct tcacagacca caaataatgt agggatgcca ggatcgcgac
     7741 aagatagacg acattacgca gcggttgctg gggattaccc aattttcact gcatcgtctg
     7801 gatagatgtg aatgctaaag cacgcaaaat caaccaactg acaattctca gcattaaata
     7861 ggttcgagtg gttaatttgt agtcaaataa aatcagatta ttcaccccat ggtaccagaa
     7921 atcagtaaca ggttaagcgt agtgtcgccc aggcaaagca ccataaacct aacaggcgac
     7981 ggaggcgtat caataatggc tgtttagagg agtatcaatg cgcatcaatt tgtggtcagt
     8041 cgaaatcatg actttccagt ttggatgatg ttgtacaact tcctgaccaa ggtgttcgac
     8101 gaaagctgtc cggctaaccg cttttcccgt taaccgattg aataatgatg tcaaaatgag
     8161 ttccctctct tattattcct tgctaaatgg ttagttaacc ttcaccagcg tgcgaccctg
     8221 gatctggtta ttaatgatgg cctcggcaaa gttcggtgcc tctgacagag atatctcttt
     8281 tgccgcctgg gtatagaatg attccggtaa atcggcgacc agtcgctgcc aggcttgtgc
     8341 gcggcgttct ggtggcgtca ttactgaatc caccccttgc aaacggacat tacgcagaat
     8401 aaatggcatg accgtggttg gcagagtaaa accacccgcc agaccacagg ccgccacgca
     8461 gccgccgtaa ttcatttgcg ccagcacttt tgccagcact ttgtcgccaa cagtgtcaat
     8521 tgccccagcc cagacttgtt tttccagagg acgggattcg gcaaactcat cacgagggag
     8581 aataccgctg gcacctaaac ttttcagata ttcatgggta ctttcgcgac cggaaacggc
     8641 aacgacctga taacccaact tatgcagcag cgccacggcg gtactgccga cgccaccact
     8701 ggcacccgtc acgacaatct ccccgtcctg cgggcgaaca ccggcatctt ccagcgccat
     8761 cacacacagc atggcggtaa aaccggcagt accgataatc attgctttac gcgcgtccag
     8821 cccttgcggc atggcaacca gccagtcacc tttcactcgc gcctgctccg ccagcccacc
     8881 ccagtggttt tcaccaacgc cccagccagt gagtaacacc tcctgaccgg catgaaaacg
     8941 cggatcttcg ctggtgcgta cagttccggc aaaatcgatc ccaggaatca tcggaaaatt
     9001 acggatgatt tttcccttac cggtaatcgc cagcgcatct ttatagttca ggctcgacca
     9061 gtgaacatcg accgtgacat cgccctccgg caggcgacgt tcgtccagag tctgtactga
     9121 tgcgagagtt ttgccgtcct gctgttctaa aagtaacgcc tgcataagtg gtcctcatgt
     9181 gcatgatgga ttggaaaata atttctgaag actatactcg ctaatggaaa agcaaagccg
     9241 atgaagcgca ataaattgcg agataaatct gatttgctag tatgcccgct tcctcactat
     9301 cggagttaac acaaggatga gattaacgac gaaattttcg gcctttgtta cgctgctcac
     9361 cgggttaaca atttttgtga ctttgctggg ctgttcgcta agtttctaca acgccattca
     9421 gtataagttt agtcatcgcg ttcaggcggt ggcgacggcg attgataccc accttgtgtc
     9481 gaatgacttc agcgcattaa ggccacaaat taccgaatta atggtgtcag cagatatcgt
     9541 tcgtgtagac ctgctgcatg gtgataaaca ggtttatacc ctggccagaa atggtagtta
     9601 tcgtccggtt ggcaccagcg atctgtttcg tgaactgagc gttccgttga taaagcatcc
     9661 ggggatgtcg ttgcgtctgg tttatcagga tccgatgggc aactatttcc attcgttgat
     9721 gaccaccgcg ccgctcacgg gggcgattgg ctttatcatt cttatgctct ttctggcggt
     9781 acgctggtta caacggcaac ttgccgggca agaattgctg gaaacccggg ctactcgtat
     9841 cttaaacggt gagcgtggct ctaatgtgtt gggaaccatc tatgaatggc cgcccagaac
     9901 cagcagtgcg ctggatacgc tgcttcgtga aattcagaac gcacgcgaac aacacagccg
     9961 tcttgatacg ctgatccgct cttatgccgc acaggacgtg aaaaccggcc tcaataaccg
    10021 actctttttc gataatcagt tagcaacgtt actggaagat caggagaaag taggtaccca
    10081 cgggatcgtg atgatgattc gtctgccgga tttcaatatg ttgagcgata cctgggggca
    10141 cagccaggtt gaagaacagt tcttcactct gacgaatctg ctgtcgacat ttatgatgcg
    10201 ctaccctggc gcactgctgg cgcgttacca ccgcagtgat tttgctgcgc tgttaccgca
    10261 ccggacgtta aaagaggcag agagcatagc cagtcagtta atcaaagccg tcgatacctt
    10321 gccgaacaat aaaatgctcg atcgcgacga tatgatccac attggtatct gtgcctggcg
    10381 tagtggtcag gataccgagc aggtaatgga acatgcagag tctgccacgc gtaatgcggg
    10441 attgcagggt ggcaatagct gggctattta tgatgactcg ttgcctgaaa aaggacgcgg
    10501 taatgttcgc tggcgtacgc ttatcgagca aatgctcagt cgcggcggcc cgcgccttta
    10561 tcaaaaaccg gcggttactc gcgaaggtca ggttcatcat cgcgaactca tgtgccgcat
    10621 cttcgatggt aatgaagagg ttagctcggc ggagtatatg ccgatggtct tgcagtttgg
    10681 cttatcggaa gagtatgacc gtctgcaaat cagccgtctt attccactat tgcgttactg
    10741 gccagaggaa aatctggcga ttcaggttac cgttgagtcg ctgattcgtc cgcgttttca
    10801 gcgttggctg cgcgatacgt taatgcaatg tgaaaaatca caacgaaaac gcataattat
    10861 tgaacttgca gaggccgatg taggtcaaca tatcagtcgt ttacaacctg ttattcgttt
    10921 agtgaatgct ttaggggtac gggtagccgt caaccaggct ggtttgacgc tggtaagcac
    10981 cagttggatc aaagaactta atgttgagtt actcaagctc catccggggc tggtcaggaa
    11041 cattgagaag cgaacggaga accagctgct ggttcaaagc ctggtggaag cctgctccgg
    11101 gaccagcacc cacgtttacg ccaccggcgt gcgttcgcga agcgagtggc agaccctgat
    11161 tcagcgcggt gttacaggcg ggcaagggga ttttttcgcg tcctcacagc cacttgatac
    11221 taacgtgaaa aaatattcac aaagatactc ggtttaacct gccgtttaat ccgttttcac
    11281 gtagaataac gcgcgctgcg tctcatggga gtgtgcttgt ctgctcgcca gattgttgca
    11341 gcacatatgc agatgaatga ccttacgcgg ttgcaaacag gcgaggaatg ctgctgatgc
    11401 attaagcctt tctggactca ggcagagatt tgtaacaaag gaaacgaact gcactaattt
    11461 tcaccgtagc agatgatttt tgcgccttgt cgctgctgcg tgtggttggt aaagtaagcg
    11521 gattttcttt tccgccccag ctttcaggat tatcccttag tatgttgaaa aaatttcgtg
    11581 gcatgttttc caatgacttg tccattgacc tgggtactgc gaataccctc atttatgtaa
    11641 aaggacaagg catcgtattg aatgagcctt ccgtggtggc cattcgtcag gatcgtgccg
    11701 gttcaccgaa aagcgtagct gcagtaggtc atgacgcgaa gcagatgctg ggccgtacgc
    11761 cgggcaatat tgctgccatt cgcccaatga aagacggcgt tatcgccgac ttcttcgtga
    11821 ctgaaaaaat gctccagcac ttcatcaagc aagtgcacag caacagcttt atgcgtccaa
    11881 gcccgcgcgt tctggtttgt gtaccggttg gcgcgaccca ggttgaacgc cgcgcaattc
    11941 gtgaatccgc gcagggcgct ggtgcccgtg aagtcttcct gattgaagaa ccgatggctg
    12001 ccgcaattgg tgctggcctg ccggtttctg aagcgaccgg ttctatggtg gttgatatcg
    12061 gtggtggtac cactgaagtt gctgttatct ccttgaacgg cgtggtttac tcctcttccg
    12121 tgcgcattgg tggtgatcgt ttcgacgaag ctatcatcaa ctatgtgcgt cgtaattacg
    12181 gttctctgat cggtgaagcc accgcagaac gtatcaagca cgaaatcggt tcggcttacc
    12241 cgggcgatga agtccgtgaa atcgaagttc gtggccgtaa cctggcagaa ggtgttccac
    12301 gcggttttac cctgaactcc aacgaaatcc tcgaagcact gcaggaaccg ctgaccggta
    12361 ttgtgagcgc ggtaatggtt gcactggaac agtgcccgcc ggaactggct tccgacatct
    12421 ccgagcgcgg catggtgctc accggtggtg gcgcactgct gcgtaacctt gaccgtttgt
    12481 taatggaaga aaccggcatt ccagtcgttg ttgctgaaga cccgctgacc tgtgtggcgc
    12541 gcggtggcgg caaagcgctg gaaatgatcg acatgcacgg cggcgacctg ttcagcgaag
    12601 agtaatcgga tgcaggcagg ggaagcgtct gtttaccctg cctggtctga tacgagaata
    12661 cgcataactt atgaagccaa tttttagccg tggcccgtcg ctacagattc gccttattct
    12721 ggcggtgctg gtggcgctcg gcattattat tgccgacagc cgcctgggga cgttcagtca
    12781 aatccgtact tatatggata ccgccgtcag tcctttctac tttgtttcca atgctcctcg
    12841 tgaattgctg gatggcatat cgcagacgct ggcctcgcgt gaccaactag aacttgaaaa
    12901 ccgggcgtta cgtcaggaac tgttgctgaa aaacagtgaa ctgctgatgc ttggacaata
    12961 caaacaggag aacgcgcgtc tgcgcgagct gctgggttcc ccgctgcgtc aggatgagca
    13021 gaaaatggtg actcaggtta tctccacggt taacgatcct tatagcgatc aagttgttat
    13081 cgataaaggt agcgttaatg gcgtttatga aggccagccg gtcatcagcg acaaaggtgt
    13141 tgttggtcag gtggtggccg tcgctaaact gaccagtcgc gtgctgctga tttgtgatgc
    13201 gacccacgcg ctgccaatcc aggtgctgcg caacgatatc cgcgtaattg cagccggtaa
    13261 cggttgtacg gatgatttgc agcttgagca tctgccagcg aatacggata ttcgtgttgg
    13321 tgatgtgctg gtgacctccg gtctgggcgg tcgtttcccg gaaggctatc cggtcgcggt
    13381 tgtctcttcc gtaaaactcg atacccagcg cgcttatact gtgatccagg cgcgtccgac
    13441 tgcaggactg caacgtttgc gttatctgct gctgctgtgg ggcgcggatc gtaacggcgc
    13501 taacccgatg acgccggaag aggtgcatcg tgttgctaat gaacgtctga tgcagatgat
    13561 gccgcaggta ttgccttcgc cagacgcgat ggggccaaag ttacctgaac cggcaacggg
    13621 gatcgctcag ccgactccgc agcaaccggc gacaggaaat gcagctactg cgcctgttgc
    13681 gccgacacag cccgctgcta atcgctctcc acaaagggct acgccgccgc aaagtggtgc
    13741 tcaaccgcct gcgcgtgcgc cgggagggca atagtggcga gctatcgtag ccagggacgc
    13801 tgggtaatct ggctctcttt cctcattgcg ctgttgctgc aaatcatgcc ctggccggat
    13861 aacctgattg ttttccggcc aaactgggtg ttacttatct tgttgtattg gatcctggcc
    13921 ttgccccatc gcgtaaatgt gggcacaggt tttgtgatgg gtgccatact ggatctgatc
    13981 agcggctcga tgcttggcgt acgcgtattg gcgatgagca tcattgctta cctggtggcg
    14041 ctgaaatacc agcttttccg caacctcgca ttatggcagc aggcgctggt cgtcatgttg
    14101 ctttcgctgg tggtggatat tattgttttc tgggcagagt ttttagtgat taacgtctct
    14161 ttcagaccgg aagtgttctg gagtagtgta gtcaatgggg tgctctggcc gtggattttc
    14221 ttgctgatgc gtaaagtccg tcagcagttt gcagtgcaat aaaggtttct atgacttctc
    14281 tgtatttagc ttccggttct ccgcgtcgtc aggagttact tgcgcaactt ggcgtgacct
    14341 ttgaacgtat tgttacgggc attgaggagc agcgtcagcc gcaggagagc gcgcagcagt
    14401 atgttgtgcg tctggcacgc gagaaagcac aggcgggtgt cgcgcaaacg gcacaggatc
    14461 tcccggtgct gggtgcggat attatcgtta tcctgaacgg agaagtgctc gagaaaccgc
    14521 gcgacgcaga gcatgcggcg cagatgttgc gcaaattatc gggtcagacc catcaggtga
    14581 tgacggcagt ggcgttggcc gacagccagc acattctcga ttgcctggtg gtcaccgatg
    14641 tgactttcag aacgttaaca gacgaagaca tcgcgggcta tgtcgccagc ggtgaaccgt
    14701 tagataaagc aggtgcatac ggtattcagg ggctgggtgg ctgttttgtc aggaagataa
    14761 atggcagcta tcacgccgta gtcggcttac cgctggttga aacgtatgaa ttattaagta
    14821 attttaacgc actgcgtgag aaaagggata aacatgacgg ctgaattgtt agtaaacgta
    14881 acgccttcgg aaacgcgagt ggcgtatatt gatggcggta ttctgcagga aattcatatt
    14941 gaacgtgagg cgcgacgcgg aatagtaggc aatatctaca agggtcgtgt aagtcgtgta
    15001 cttccgggta tgcaggcggc ttttgtagat attgggctgg ataaagccgc gtttcttcat
    15061 gcatccgaca tcatgccgca taccgaatgt gtggcgggtg aagaacaaaa gcaattcacg
    15121 gtgcgcgaca tctcggaact ggttcgtcag gggcaagatc tgatggtgca ggtggtgaaa
    15181 gatccgcttg gcactaaagg tgcgcgcctg accaccgata tcacgctgcc ttctcgctat
    15241 ctggtattta tgccaggggc ttctcacgtt ggggtttccc aacgaattga aagcgaatca
    15301 gaacgtgaac gtctgaaaaa agtggtcgca gagtattgcg acgagcaggg cgggtttatc
    15361 atccgcaccg cagcggaagg ggttggcgag gctgaactgg cctccgatgc cgcttatctg
    15421 aaacgcgtct ggaccaaagt tatggagcgt aaaaagcgcc cgcagacccg ttatcagctg
    15481 tacggcgaac tggcgctggc gcagcgtgtt ctgcgtgatt ttgccgacgc cgaactggac
    15541 cgcattcgcg ttgactcacg cctgacttac gaagcattgc tggagtttac ctcggagtac
    15601 attcccgaga tgaccagcaa gctggagcat tacaccggac gccagccgat tttcgatctc
    15661 tttgatgtcg aaaacgaaat ccagcgagcg ttggaacgca aagtagaact gaaatccggt
    15721 ggttatctga ttatcgacca gaccgaagcg atgaccaccg tggacatcaa taccggagcg
    15781 tttgtcggtc atcgcaatct ggacgacact attttcaata ccaatattga agcgacgcag
    15841 gctatcgctc gccagttacg gttgcgtaat ctgggcggga ttatcattat tgatttcatc
    15901 gatatgaata atgaagatca ccgccgccgc gtgctgcatt cgctggagca ggcgttgagc
    15961 aaagaccggg tgaaaaccag cgttaatggt ttttcggcgc tggggctggt ggagatgacg
    16021 cgtaaacgca cccgcgaaag cattgagcac gtactgtgta acgaatgccc aacctgccac
    16081 ggtcgcggaa cggtgaaaac cgtggaaacg gtatgctatg aaatcatgcg cgagattgtt
    16141 cgtgtccacc atgcttacga ctccgaccgt ttcctggtct atgcttctcc ggcagtagcc
    16201 gaagccttga aaggcgaaga gtcacactcg ctggcggaag tggaaatttt cgttggcaaa
    16261 caggttaaag tacaaattga accgctctat aaccaggagc agtttgacgt cgtaatgatg
    16321 taaacagatg ctgggccgcc atccggcaaa gggtttttga gtcacatttt tagcagacaa
    16381 ggagtgacgg gtgaggcgat tgccggggat tttactgctt actggagccg cgctcgttgt
    16441 gatcgctgcc ctgctggtta gcggcctgcg tattgcttta ccgcatctta acgcctggcg
    16501 tccggaaatc ctcaacaaaa tagaatccgc gactggcatg ccggtagaag ccagtcagct
    16561 ctcagccagc tggcagaatt ttggcccgac gcttgaagca cacgacatcc gtgcagaact
    16621 aaaagatggc ggcgaatttt cggttaaacg cgttactctg gcgctggatg tctggcagag
    16681 cctgttacat atgcgctggc agtttcgcga cctcactttc tggcagctgc gctttcgcac
    16741 caacactcct atcaccagcg gtggtagcga tgacagtctg gaagccagtc acatcagcga
    16801 tctgtttctt cgtcaatttg accatttcga tctccgcgac agtgaagtca gttttctgac
    16861 gccatccggt cagcgcgccg agctggcgat cccacaactc acctggctga acgatccacg
    16921 tcgacaccgt gcggaaggcc tggtaagcct ctccagcctt accggacagc acggcgtgat
    16981 gcaggtgcgc atggatttgc gcgatgatga ggggttgtta agcaatggtc gcgtctggct
    17041 acaggcggat gacatcgacc tgaagccgtg gctcggtaaa tggatgcagg acaatattgc
    17101 tctggaaacg gcacagttct cccttgaagg ctggatgacg atcgacaaag gcgatgtaac
    17161 cggcggtgac gtctggctga aacagggcgg tgccagctgg ttgggcgaga agcaaacgca
    17221 tacgctgtcg gtggataatc tgaccgcgca tattacgcgt gaaaatccgg gctggcagtt
    17281 ctctattccc gatacacgga tcacgatgga cggcaaaccc tggccgagcg gagcattgac
    17341 gctggcctgg ataccggaac aggacgttgg cggcaaagac aataaacgca gtgacgaact
    17401 ccggattcgc gctagtaatc tggagctggc aggcctggag ggcatacgcc cgctggccgc
    17461 gaaactttca cctgcactgg gtgatgtttg gcgctccaca caaccgagcg gcaagattaa
    17521 cactctggcg ctggatatcc cgcttcaggc ggcagacaag acccgttttc aggcatcgtg
    17581 gagcgatctg gcctggaagc aatggaaatt attaccgggt gcggaacact tctccgggac
    17641 gctttccggc agcgttgaaa atggtttgct taccgcgtcg atgaagcagg caaagatgcc
    17701 ttacgaaacg gtattccgtg cgccactgga aatcgccgac ggccaggcaa ctataagctg
    17761 gctgaacaat gacaaaggtt tccagctgga tgggcgtaat attgacgtta aagccaaagc
    17821 cgtccatgcg cgcggcggtt ttcgttacct gcaacctgct aacgatgaac cctggctggg
    17881 tattctggct ggcatcagta ccgatgatgg ttcacaagcc tggcgctatt tcccggaaaa
    17941 cttgatgggt aaagagttgg ttgattattt aagtggtgcg attcagggcg gtgaagcgga
    18001 taacgcgacg ctggtttatg gtggcaatcc gcaactcttc ccctataaac acaacgaagg
    18061 tcagtttgaa gtgctggtgc cgctgcgcaa cgcgaagttt gccttccagc cggactggcc
    18121 tgcattaact aaccttgata ttgaactgga ctttattaac gacggtttat ggatgaaaac
    18181 cgatggcgtt aatctgggcg gcgtgcgcgc gagtaatctc accgcagtga tccctgacta
    18241 ctcaaaagaa aaactgctga ttgacgctga cattaaaggt ccgggtaaag ccgttggccc
    18301 ttactttgat gagacaccgc tgaaagattc tctgggtgcg accctgcaag aactccagct
    18361 cgacggcgat gtgaatgctc gcttacatct tgatatcccg ctgaacggcg aactggtaac
    18421 cgcgaaaggt gaagtgacgc tgcgtaataa cagtctgttt atcaaaccac tcgacagcat
    18481 cctgaaaaat ttgagcggta aattcagctt tatcaatggc gatctgcaaa gtgaaccact
    18541 gacagcaagc tggtttaatc agccgttgaa cgtggatttt tccaccaaag aaggggcaaa
    18601 agcctaccag gttgcggtga atctcaacgg taactggcaa ccggcgaaaa ccggcgttct
    18661 gcctgaagcg gtgaacgaag cattgagtgg cagcgtggcg tgggatggta aagtgggcat
    18721 tgatctgcct tatcatgctg gcgcgaccta taacgtagag ctgaacggcg atctgaataa
    18781 tgtgagtagt cacttacctt caccgttagc caaacctgcg ggtgaaccac tagcggtaaa
    18841 cgttaaggtt gatggcaatc tcaacagctt tgaattaacc ggacaggctg gtgcggataa
    18901 ccatttcaat agccgctggt tgcttggtca aaagctgacg ctcgatcgtg ctatttgggc
    18961 ggcagacagt aaaacgctcc cgccgttgcc ggaacaaagt ggcgttgaac ttaatatgcc
    19021 gccgatgaat ggtgccgagt ggctggccct gttccagaaa ggcgctgcgg agagtgtcgg
    19081 tggtgcagcg agtttcccac aacacataac gttacgtacg cctatgttgt cactgggaaa
    19141 tcagcaatgg aataacctga gtattgtttc gcaaccgacg gcaaatggca ccctggttga
    19201 ggcgcaaggg cgtgaaatca acgccacgct agcgatgcgt aataacgcgc cgtggctggc
    19261 gaatatcaaa tatctttatt acaacccgag cgtggcgaaa actcgtggtg attcaacgcc
    19321 gtcatcacct ttcccgacaa cggagcgcat taacttccgt ggctggccgg acgcacaaat
    19381 acgatgcaca gagtgctggt tctgggggca aaaattcggt cgcattgaca gtgatatcac
    19441 catttctggc gatacgttaa cgctgaccaa tggactgatt gatactggtt tctcgcggct
    19501 tactgccgat ggtgaatggg ttaataatcc ggggaatgaa cgtacctcgc tgaaaggaaa
    19561 actgcgtggg cagaaaattg atgccgccgc agaatttttt ggtgtcacga cgcccatacg
    19621 ccagtcgtca tttaatgtgg attacgattt acactggcgc aaagcaccct ggcagccaga
    19681 tgaagcgacg ttgaatggca tcattcatac tcaactgggt aaaggcgaaa ttaccgaaat
    19741 caataccgga catgccgggc aattgctgcg cttattgagc gtagatgccc tgatgcgtaa
    19801 gctgcgtttt gatttcagag acacttttgg cgaagggttc tattttgact ccattcgcag
    19861 caccgcgtgg attaaagacg gcgttatgca caccgacgac acgctggtgg atggcctgga
    19921 ggcggatatc gccatgaaag ggtcggtaaa tctggtacgc cgcgacctga atatggaagc
    19981 ggttgtcgca ccagagattt ctgcgacggt gggcgtggct gcggcttttg cggttaaccc
    20041 cattgttggc gcggcagtgt ttgccgccag taaagtgctg gggccgctgt ggagcaaagt
    20101 ctccattttg cgctatcaca tttcgggtcc gctggacgat ccgcaaatca acgaagtgtt
    20161 gcgccaaccg cgtaaagaaa aagcgcaatg atttgacgag ggcgcgtaat tgccccaatc
    20221 tcatatgata atcgttgcca aaggccaacg agccggaaca taactgtagg ccggataggg
    20281 cgttcacgcc gcatccggca gccgtaaaaa atcctctact gcagtaacta acgagtagca
    20341 aaaacgatga gtctgaacct ggtaagtgaa caattgctag cggcgaacgg cctgaaacat
    20401 caggacttgt tcgcgatcct cggtcaactg gccgaacgtc gccttgatta tggcgatctc
    20461 tattttcagt cgagctatca cgaatcctgg gttttagaag accgcattat taaagatggt
    20521 tcttacaaca tcgatcaggg cgttggcgtg cgtgcaatca gcggtgaaaa aaccggattt
    20581 gcttacgctg accaaatcag cctgctggcg ctggaacaga gtgcgcaagc ggcgcgcacc
    20641 atcgtccgtg atagtggtga tggcaaagta cagacgctgg gcgcggtaga gcatagcccg
    20701 ttgtatacct cggtagatcc gctgcaaagc atgagccgtg aagagaagct ggatatcctg
    20761 cgtcgcgtcg ataaggttgc tcgcgaagcg gacaagcgcg tacaggaagt gactgccagc
    20821 ctcagtggtg tctatgaatt aattctggtt gcggccaccg acggcacgct agcggcggat
    20881 gtccgtccgc tggtgcgtct ttccgtgagc gttctggtcg aagaagatgg caaacgcgaa
    20941 cgcggtgcca gtggcggcgg cggtcgtttt ggttatgagt tcttccttgc cgatcttgac
    21001 ggcgaagtcc gtgcggatgc atgggcaaaa gaagcagtgc gtatggcgct ggtcaatctt
    21061 tctgccgttg ctgcaccagc gggcaccatg ccggtagtac ttggcgcagg ttggccgggc
    21121 gtgctgttgc atgaagcggt aggtcacggt ctggaaggcg acttcaaccg ccgtggcact
    21181 tcagtattta gtggacaggt cggggagctg gtggcttcag aactgtgtac cgtggttgat
    21241 gacggcacga tggtcgatcg ccgtggttcg gtggcgattg atgatgaagg tacgccaggc
    21301 cagtacaacg tgctgattga gaacggcatt ctgaaaggct acatgcagga taaactcaac
    21361 gcgcgtttga tggggatgac gccgactggc aacggtcgcc gtgaatccta cgcccatctg
    21421 cccatgccgc gtatgaccaa cacctatatg ctgccgggta aatcgacccc gcaagaaatt
    21481 attgaatccg ttgagtacgg tatctatgca ccgaactttg gtggcggtca ggtggatatc
    21541 acctccggca aattcgtttt ctccacttca gaagcatatc tgattgaaaa cggtaaagta
    21601 acgaagccgg tgaaaggcgc aacgttgatt ggttccggta tcgaaaccat gcagcagatt
    21661 tcgatggttg gcaacgacct gaaactggat aacggcgtgg gtgtctgcgg taaagaaggg
    21721 caaagtttgc cggttggcgt gggccagcca acgttgaaag tcgataacct gactgttggc
    21781 ggtactgcgt aataatttca ttattttcag aaggataatt aatctttcta cgtgcacgaa
    21841 cggtcccctc gctcctctgg ggttagggtg aggggaaccc gttggcacag gtttgtagaa
    21901 cgtaacagta caatatgaat tacttctctt tcccgcgccc gtgcatctcc tgaaacaatt
    21961 taccgacctc aacaaaataa tccgtcagcg agttgatcac gacctgtacc ttcagcggca
    22021 gcttatcttt ttcggtatat aacgcataaa ccgggcgtgg atctgactga taacgcggca
    22081 gcaggatctc cagctcccca cgattgatct cgttgatcac ccacatcagc ggcacgtagg
    22141 cgatcccggc acccgccgtt agccagcgca ccagcgtcat cggatcatta gtcacaaatc
    22201 tcccttgcgg gatcaggcga gtcgagatcc cttccggtgc aatcagttca aattcattgt
    22261 cgggccgcac gctgtattca agccatgaat ggctacttaa atcggcgggt ttttccggta
    22321 tgccgtattg tgtgagatag cttttcgcgg cgcacaccac cattggcatc gcgccaagac
    22381 ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg gatcaccaca tccagaccgt
    22441 cggcaatcag gtcgggggct ggaattccgg taaccagatt ggcgctcaaa cctgggtatt
    22501 ctttcagcat tttggctgtc agcccggcga gaacattttg tgccatagtt gaagaacagc
    22561 caatgcgtag cgtcccgatg ggggtgttat tgaaggcata cagttgctca tgaacatcct
    22621 gcacttcatg aagcatacga cggcagccct ggtagtaaat tctaccggct tcggtcaggc
    22681 caatgctgcg tgtgctacgg tttaacagct ttacctgcaa ctcatcttcc agttttgaca
    22741 ccgtctgact gatggacgaa acgctcatct gtagctgtct ggcggcggcg gtaaaagagc
    22801 caaattcaac tactttggca aacaccgaca tgcgttttag tcgttccatt attcactctg
    22861 acttaaaagt gatttagatc acataatata gataacagca taacagttac gctaatatat
    22921 taaatatcaa tctacagcaa tgttgctctc gcccggcttt ccatgccctt ctctgcggcg
    22981 acagatgctg aaaataataa cgcctgctct ctctatacaa ccaaggtcaa catgagtctg
    23041 tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga tattttttga attgctttta
    23101 tcactggcga ttttctggct ggtgcgccgg gtacttgtgc caacaggtat ctacgacttt
    23161 gtctggcatc cggcgttgtt caacaccgcg ctctattgct gcttgtttta tttgatatcg
    23221 cgactgttcg tttgaggttg aagtgaaaac actaataaga aaattctccc gtacggccat
    23281 cacggtcgta ttagtcattc tggccttcat cgcaattttt aatgcctggg tctattacac
    23341 cgaatccccc tggacgcgtg acgcgcgctt tagcgctgac gtcgttgcga tcgcgccgga
    23401 cgtttctgga ctcattaccc aggtgaatgt tcatgataac cagctggtga aaaaaggaca
    23461 gatactgttc accatcgacc agccgcgcta tcaaaaggcg cttgaggaag cgcaagccga
    23521 tgttgcttat tatcaggtac tggcacagga gaaacgccag gaggccggac gtcgtaaccg
    23581 tctcggtgtg caggcgatgt ctcgcgaaga gatcgaccag gctaacaacg tactacaaac
    23641 ggttctgcat cagttagcga aagcgcaggc gacccgcgat ctggcaaaac tggatcttga
    23701 acgcacggtg atccgagcgc cagcagatgg ctgggtgacc aacctcaacg tctataccgg
    23761 tgagtttatt actcgaggat caacggcggt tgcgctggtg aaacagaact ccttctatgt
    23821 actggcctat atggaagaaa ctaagctgga aggggtgcat ccggggtatc gtgcagagat
    23881 cacgccgctt ggcagtaaca aagtgctgaa agggactgtt gatagtgttg ccgctggggt
    23941 caccaacgcc agcagcacgc gtgacgacaa agggatggcg accatcgact ctgaccttga
    24001 atgggtgcgt ttggcgcaac gtgttccggt tcgtattcgt ctcgacaacc agcaagagaa
    24061 catctggcct gcgggcacca ctgctacagt ggtggtcact ggcaaacaag atcgcgacga
    24121 aagccaggat tcgttcttcc gtaaaatggc ccatcgcctg cgtgagtttg gttaatcacg
    24181 atgggtattt tctccattgc taaccaacat attcgctttg cggtaaaact ggcgaccgcc
    24241 attgtactgg cgctgtttgt tggctttcac ttccagctgg aaacgccacg ctgggcggta
    24301 ctgacagcgg cgattgttgc cgccggtccg gcctttgctg cgggaggtga accgtattct
    24361 ggcgctattc gctatcgtgg ctttttgcgc atcatcggca catttattgg ctgtattgcc
    24421 ggactggtga tcatcattgc gatgatccgc gcaccattat tgatgattct ggtgtgctgt
    24481 atctgggccg gtttttgtac ctggatatcc tcgctggtac gaatagaaaa ctcgtacgcg
    24541 tgggggctgg ccggttatac cgcgctgatc attgtgatca ccattcagcc ggaaccattg
    24601 cttacgccgc agtttgccgt cgaacgttgt agcgagatcg ttatcggtat tgtgtgtgcg
    24661 attatggcgg atttgctctt ttctccgcga tcgatcaaac aagaagtaga tcgagagctg
    24721 gaaagtttgc tggtcgcgca atatcaatta atgcaactct gtatcaagca tggcgatggt
    24781 gaagttgtcg ataaagcctg gggcgacctg gtgcgacgca ccacggcact acaaggcatg
    24841 cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg ccaatcgacg tttaaaagcg
    24901 atcaatacgc tatcgctgac gctgattacc caatcctgcg aaacttatct tattcagaat
    24961 acgcgcccgg aattgatcac tgatactttc cgcgaatttt ttgacacgcc ggtagaaacc
    25021 gcgcaggacg tccacaagca gctcaaacgc ctgcggagag ttatcgcctg gaccggggaa
    25081 cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg caacgcgtta tcagcttctc
    25141 aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg aagaagagat cctgcaaggc
    25201 gaaccggaag taaaagtaga gtcagccgaa cgtcatcatg caatggttaa cttctggcga
    25261 accacacttt cctgcattct gggcacgctt ttctggctgt ggacgggctg gacttccggc
    25321 agtggtgcaa tggtgatgat tgcggtcgtg acgtcactgg caatgcgttt gccgaatcca
    25381 cgcatggtgg cgatcgactt tatctatggg acgctggccg cgctgccgtt agggctgctc
    25441 tactttttgg tgattatccc taatacccaa cagagcatgt tgctgctgtg tattagcctg
    25501 gcagtgctgg gattcttcct cggtatagaa gtacagaaac ggcgactggg ctcgatgggg
    25561 gcactggcca gcaccataaa tattatcgtg ctggataacc cgatgacttt ccatttcagt
    25621 cagtttctcg acagcgcatt agggcaaatc gtcggctgtg tgctcgcgtt caccgttatt
    25681 ttgctggtgc gggataaatc gcgcgacagg actggacgtg tactgcttaa tcagtttgtt
    25741 tctgccgctg tttccgcgat gactaccaat gtggcacgtc gtaaagagaa ccacctcccg
    25801 gcactttatc agcagctgtt tttgctgatg aataagttcc caggggattt gccgaaattt
    25861 cgcctggcgc tgacgatgat tatcgcgcac cagcgcctgc gtgatgcgcc gatcccggtt
    25921 aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca cagcagacca tgtgatatct
    25981 gcccgtagcg atgataaacg tcgtcggtac tttggccagt tgctggaaga actggaaatc
    26041 taccaggaaa agctacgcat ctggcaagcg ccaccgcagg tgacggaacc ggttcatcgg
    26101 ctggcgggga tgctccataa gtatcaacat gcgttgaccg atagttaagt caaaaccgac
    26161 gccaaaagcg tcggtttttt catggctata cttagcgatg cacggcagaa ctcgccgcga
    26221 aacgtgacgg tggcaacaga tgaatattta tgcctttgat tttgatgaga ttgagagtca
    26281 ggaggatttt tatcgtgact ttagccaaac ctttggtctg gctaaagata aggtacgcga
    26341 tctcgactca ctatgggatg tgttaatgaa cgatgtcctg ccgctaccac ttgagattga
    26401 atttgttcat ctgggagaga aaacgcgtcg ccgttttggc gcgttaatat tgctgtttga
    26461 tgaggcagag gaagagctgg aagggcattt gcgttttaat gttcgtcatt agcgcaaaaa
    26521 aaagcccacg aaccgggggc aatatcgtcg gacaaaacga tgagggttta tttgtacagt
    26581 tcagccgtag cgtgccaggt gtcaccgcta cgagcttcag taatctggta ggccgttgcg
    26641 cctttctctt ccgctttttt gttcagcatt tcacgtatat ccattggcga agacgccaca
    26701 ccacttacgg atacggtccc gattgcttca cgattttgtg cttgtgcagc atcaatggag
    26761 tcggcagcga atgcaccgaa agagagaaca gaaagtacgc ttaatgcagc tacagtggtt
    26821 ttgattttca tgatttttac ctcgacataa tcttttagct gggcctttgt ttcgtgaccc
    26881 atatcacaaa atcaagtata cactaattat tgaactaatt aataccacac taactgtttc
    26941 agttgatact aagtgttaaa aatgtgaatt ttaataacaa aaaagtataa aaaacacggt
    27001 tatttattat taattatagt attttcaatt agataatgaa ttttgcataa tgtgcttttt
    27061 ttaccgtatg tattcatttt gtgaatgatg tgtcgcggta aaacgcacta ttcgttaaat
    27121 gtatcgcagg agaaagaagg ggaatgagag ggataagcaa cattttcccc gccgccagaa
    27181 gcgacggggc agagattaaa gctcctggtc gaacagctct aaaatcgctt cgtacaggtc
    27241 tttgacggtg aaaccgttag caggggtggt aaagatggtg tcatcgccag cgatggtgcc
    27301 cagaatacct tctgctttgc ccagtgagtc cagcaggcga gcaattaact gcgccgcgcc
    27361 agggctggta tgaatcacga caactgcatc gttgtagtcg atatccagca ccagattctt
    27421 caatggactg gaggtggttg gtacacccag ttcagctggc aggcagtaaa ccatttccat
    27481 tttggcattg cgtgtacgta cagcaccaaa cttggtcaac atccgcgaga ctttagactg
    27541 attaatattg tcaaagcctt gctcctgcaa cgcggcgacg atttcgccct gggagctaaa
    27601 tttctcttct ttaagtaatg ctttaaatgc tttaactagt tcttcttgct tagccgagct
    27661 tcgcataagt cacccgatat ggtggttgat acaacattat tgtgcataca gatgaatttt
    27721 tatgcaaaca gtcagccctg aagaaggctg aaataatgtt atgaaagagc gggattttat
    27781 caaatttcgt tattgagaaa catgcctgcg tcacggcatg caaattctgt ttaaaagtaa
    27841 attaattgtt atcaaattga tgttgttttg gctgaacggt agggtatatt gtcaccacct
    27901 gttggaatgt tgcgctaatg cataagcgac tgttaattac gtaagttagg ttcctgatta
    27961 cggcaattaa atgcataaac gctaaacttg cgtgactaca cattcttgag atgtggtcat
    28021 tgtaaacggc aattttgtgg attaaggtcg cggcagcgga gcaacatatc ttagtttatc
    28081 aatataataa ggagtttagg atgaaagtcg cagtcctcgg cgctgctggc ggtattggcc
    28141 aggcgcttgc actactgtta aaaacccaac tgccttcagg ttcagaactc tctctgtatg
    28201 atatcgctcc agtgactccc ggtgtggccg tcgatctgag ccatatccct actgctgtga
    28261 aaatcaaagg tttttctggt gaagatgcga ctccggcgct ggaaggcgca gatgtcgttc
    28321 ttatctctgc aggcgtagcg cgtaaaccgg gtatggatcg ttccgacctg tttaacgtta
    28381 acgccggcat cgtgaaaaac ctggtacagc aagttgcgaa aaactgcccg aaagcgtgca
    28441 ttggtattat cactaacccg gttaacacca cagttgcaat tgctgctgaa gtgctgaaaa
    28501 aagccggtgt ttatgacaaa aacaaactgt tcggcgttac cacgctggat atcattcgtt
    28561 ccaacacctt tgttgcggaa ctgaaaggca aacagccagg cgaagttgaa gtgccggtta
    28621 ttggcggtca ctctggtgtt accattctgc cgctgctgtc acaggttcct ggcgttagtt
    28681 ttaccgagca ggaagtggct gatctgacca aacgtatcca gaacgcgggt actgaggtgg
    28741 ttgaagcgaa agccggtggc gggtctgcaa ccctgtctat gggccaggca gctgcacgtt
    28801 ttggtctgtc tctggttcgt gcactgcagg gcgaacaagg cgttgtcgaa tgtgcctacg
    28861 ttgaaggcga cggtcagtac gcccgtttct tctctcaacc gctgctgctg ggtaaaaacg
    28921 gcgtggaaga gcgtaaatct atcggtaccc tgagcgcatt tgaacagaac gcgctggaag
    28981 gtatgctgga tacgctcaag aaagatatcg ccctgggcaa agagttcgtt aataagtaat
    29041 tgattagcga ataataaaaa accggagcac agactccggt ttttgttttg agcactcgac
    29101 ttaattggtt gccggatatt cctgaatggt gacctgcagc gttaactgct tatcatcacg
    29161 catcactaca accgggatca ccgaaccagg gcgaatttcc gccacctgat ccatcgtctc
    29221 cagagcagag atggccggtt tgttatccac cgaaataatc agatcgttga cctgaatacc
    29281 cgcattcgcc gctgggccgt caggtgacac ttcattaacc acgatccctt gcagttgatc
    29341 tataccaccg ctctgcgcgt gcagtggtgc gatctcgcgt ccgccgatac caatgtagcc
    29401 gcggatcacg cgaccatcgc ggatcagctt atccataatt ttggttgcta attggaaagg
    29461 aatcgcaaag ccgatacctt ccggcgtttc gccatcgtta ctcttatcaa acgacagcgt
    29521 gttaatgccc atcagttctc ccagcgagtt caccagcgcg ccgccagagt taccgtggtt
    29581 aatggaagca tcggtttgta ggaagttttg ccgcccggtc gggttcagac cgattcgacc
    29641 cgtggcacta ataatccctt gggtaatggt ctgcccgagg ttgtacgggt taccgatcgc
    29701 cagtactacg tcgccaatgt gcggtacgcg acgtgcatta attggaatgg taggtaaacc
    29761 gccagtggca ttaattttaa gtaccgccag atcggttaga gagtcagatc ccaccagcaa
    29821 tgcttcaaat acacgtccat cctgtaaggc gacgatgatc tgatcggcgt cgttgatgac
    29881 gtgtttattg gtgatgatat aaccgcgttg atccatgatt acaccggatc ccagggtgcg
    29941 gatctcaagc tggttgtgag agttggtgtt caaaccacgg ttgtaaacgt taaccaccgc
    30001 tggcgcggcg cggcgaaccg ccagattata gctggcaggc gtctcatcgg tactgtcaaa
    30061 ttgcggagtg gaaagcgggt taaggctgcg cagcgaaggc atggcaacca gcagaatagc
    30121 gccgacaatt aatccaatcg caacggaacg taagagcttc acaaacatga tggaggcgtc
    30181 attaaaaaag ggaacggcag cagcatacca cgagttaacc ggacatcaca cgtcagcctg
    30241 atgcccggtt tacgacatta acgcatcagc agatagatgc tttcattgcc gcgtacaatt
    30301 tgcagggcga tgatggccgg ttttgccgcc agcactttac gcatttcagc aatcgagttc
    30361 acccgatcgc ggttgacgcc aatgatcaca tcgtcttttt gcaagccagc ctgagcagct
    30421 gggcttcctt tgacaacttc atctatctta atacctttgc cgccatcttt tagctgacca
    30481 tcgctcaacg ttgcaccttc cagcgctggc cgacgaagag gtgctggtat caagcgtcac
    30541 ttctacttcc agtggtttgc cgttacgcag caggccaagc ttcactttcg tgcccggctc
    30601 tgtagtcgcg atacgagagc gcaactcagc aaagctattc agcggtttgc cgttgaggct
    30661 ggtaataata tcgcccgctt tgacgcccgc tttcgctgag ccagaacttg gcaacacttc
    30721 gctgacaaac gcgccacgct gcacgtcaag gttgaaggct ttggcgatat cggcactcat
    30781 ctcggtgcct ttgatgccta acaaaccgcg tttgatttca ccaaagtcga taagctgctg
    30841 cgccagtgtt cgcgccatat tactggggat ggcaaatcca atcccgacgc tcccgccgcc
    30901 aggcgcaagg attgcagtgt tgatgccaat taactcaccg ttaaggttta atagtgcacc
    30961 gccggagtta ccgcggttaa tggaagcatc tgtctggata aagttttcca gaccttcaag
    31021 attcaacccg ctgcggccta atgcggaaac aatgccagag gtggcggttt gcccaaggcc
    31081 aaatgggtta ccgaccgcta cggcaaaatc accgacgcgc aatttatcgg agtcggcaat
    31141 agcgatttgc gttaatttgc tcgggttttg aatttgtaac agggcgatat cgctctggtc
    31201 atcgctacca atcagttttg catcaaactc gcgcccatca ttgagctgaa tactgatttt
    31261 ctgtgcctga ttaatcacat ggttgttggt cagcacatag cctttactgg cgttgatgat
    31321 gacaccggaa cctaaacctt cgaagggttg tgcaggttga tccggtaaat catcaccaaa
    31381 aaactttttg aattcttccg ggattttctg tccctgactg gccgttcctt ccacccgtac
    31441 gctcaccact gccggaagca ctttttccag cattggagcc agactgggga gaggggcctg
    31501 atcggcaacc tggcctggaa tcgacgcgac ggcctgaaat gacgccgaga gagttaaccc
    31561 ggcacttaac gctaatgcac tcaacagctg ggtttgtttt ttcattattc ctgctctcgt
    31621 acctgaatga taagaaaaga gattcaaaac agtttgatgt tattgaattt tcagacggct
    31681 aacaatgaga caccagatta aatgatagtc ggggagggag agaagaggag ggcgcaatgg
    31741 ctgcgcccga aaaataaatt agtcgcgctt cgcgccagta cgcagcaggc cggatgcgcc
    31801 ttcagaatag tcgcgaggca cctgcaccgg tgcctgatcg ttgctggctt cagactctgc
    31861 cagacgatta cggaacgggt ttgcttcagc agacagttcc ggcagcaggc tgctggagct
    31921 ttttgccatg tgctgataca gctggcgata gtcgtgcgcc atggtatcca gtaattccgc
    31981 gctgcgggca aagtggctaa ccagctcttc gcgatactcg tccagttcag ctttattctt
    32041 ttccagttcg tactgcaacg cctgttgctg gcgtagttta cgattaccaa aacgcatggc
    32101 cacagcacca ataatgatgc cgacgactaa cccaattagc gcatattccc aggtcatgaa
    32161 catctcccgt tgtcttttgt ttccgtaggg tgttggcttc aggctccatg cctgcggctg
    32221 attatgccac tataaccgtt aattccacag aagtggaatc ccgactgcat atcgcgtagt
    32281 gtagaacggc ctttttttcg tcaaccgtga acaacggcgc accgattatt caaggaataa
    32341 caataagatc atgcaaagcg ttaccccaac atcgcaatac ctgaaggcgc tcaatgaagg
    32401 cagccatcaa cccgacgacg ttcaaaaaga ggccgtcagc cgcctggaaa ttatttatca
    32461 agaactcatc aatagcaggc caccagcccc caggacgagt gggctaatgg cgcgggtcgg
    32521 taagctgtgg ggtaaacgcg aagacacaaa gcatacgcca gtgcgtggct tatatatgtg
    32581 gggcggtgta ggacgcggga aaacctggct gatggacctt ttctatcaaa gcctgccggg
    32641 agagcggaaa cagcgcctgc actttcaccg ttttatgctg cgggtgcacg aagagctaac
    32701 tgccttacag gggcagaccg atccgctgga aattattgcc gatcgcttta aagccgaaac
    32761 tgacgtgctc tgttttgacg aattttttgt ttctgatatt accgacgcca tgctacttgg
    32821 cggtctgatg aaagccctgt tcacccgcgg tattaccctg gtagcgacgt caaatattcc
    32881 gccggacgaa ctttatcgaa atggcctgca acgtgcgcgt tttctgcctg caatcgatgc
    32941 cattaaacag cattgtgatg taatgaacgt ggacgctggt gttgattatc gactgcgtac
    33001 actcactcag gcgcatctgt ggctttcgcc actcaacgat gaaacccggg cgcagatgga
    33061 taaactatgg ttggcgctgg cgggggtgaa acgagaaaat tcaccgacgt tagaaatcaa
    33121 ccatcggcca ttggcgacaa tgggcgtcga gaaccagacg ctggcggttt cttttactac
    33181 gctgtgcgtc gacgcccgca gtcagaatga ctatattgcg ctctcacgcc tgtttcacac
    33241 agtaatgttg tttgatgtgc cagtcatgac gcggttgatg gagagcgaag cgcggcgctt
    33301 tattgcgctg gtggatgagt tttacgagcg ccatgtcaaa ttagtggtga gtgcagaagt
    33361 gccgctgtat gaaatttatc agggtgagcg gctgaagttt gagttccagc gttgcctgtc
    33421 acgtctgcaa gagatgcaaa gcgaagagta tctgaagcgc gagcatttgg cgggttaaaa
    33481 cctgccacaa atcacaaaaa ggggtcgatc tttgaccccg acttctctat aatcctgcga
    33541 ccccacgtta caagaaagtt tttttcccaa aactttttgt gtgctggcat aggctattcg
    33601 aaggggtagg tttgccggac tttgtcgtgt gaacctcaac aattgaagac gtttgggtgt
    33661 tcaccaacgt gtaactattt attgggtaag cttttaatga aaacttttac agctaaacca
    33721 gaaaccgtaa aacgcgactg gtatgttgtt gacgcgaccg gtaaaactct gggccgtctg
    33781 gctactgaac tggctcgtcg cctgcgcggt aagcacaaag cggaatatac tccgcacgta
    33841 gataccggtg attacatcat cgttctgaac gctgacaaag ttgctgtaac cggcaacaag
    33901 cgtactgaca aagtgtacta tcaccacacc ggccacatcg gtggtatcaa acaagcgacc
    33961 tttgaagaga tgattgctcg ccgtcctgag cgtgtgattg aaatcgcggt taaaggcatg
    34021 ttgccaaaag gcccgctggg tcgtgctatg ttccgtaaac tgaaagttta cgcgggtaac
    34081 gagcacaacc acgcggcaca gcaaccgcaa gttcttgaca tctaatcggg attataggca
    34141 atggctgaaa atcaatacta cggcactggt cgccgcaaaa gttccgcagc tcgcgttttc
    34201 atcaaaccgg gcaacggtaa aatcgtaatc aaccaacgtt ctctggaaca gtacttcggt
    34261 cgtgaaactg cccgcatggt agttcgtcag ccgctggaac tggtcgacat ggttgagaaa
    34321 ctggacctgt acatcaccgt taaaggtggt ggtatctctg gtcaggctgg tgcgatccgt
    34381 cacggtatca cccgcgctct gatggaatac gacgagtccc tgcgttctga actgcgtaaa
    34441 gctggcttcg ttactcgtga cgctcgtcag gttgaacgta agaaagtcgg tctgcgtaaa
    34501 gcacgtcgtc gtccgcagtt ctccaaacgt taattggctt ctgctacggc agaaaacaat
    34561 ttgcgaaaaa acccgcttcg gcgggttttt ttatggctaa attctgaatc agcgtaaaaa
    34621 atgggaagtt gctttttgct gccgtctgaa agacaggtaa aacaaaaacc acatcgccaa
    34681 taagggtcta agtcaactat ttcagactaa agcgcatctc tttttcccca tttccggcat
    34741 ccactcacca caaaggtcgc aaaatctggt aaactatcat ccaattttct gcccaaatgt
    34801 cgggtattgc tcattttttg tttgattttc gaacaaagag agtcgttcct tattgggtaa
    34861 cacaacttct gactggccac ctggtggctg gtagcagtaa aaattctgac tatacctgga
    34921 ggttttcatg gctgtcgctg ccaacaaacg ttcggtaatg acgctgtttt ccggtcctac
    34981 tgacatctat agccatcagg tccgcattgt gctggctgag aaaggtgtaa gtttcgagat
    35041 cgaacacgtg gaaaaggaca atccgcctca ggatctgatt gacctcaacc cgaatcagag
    35101 cgttccgacc ctggtggatc gtgagctgac cctgtgggaa tctcgcatca ttatggaata
    35161 tctggatgag cgtttcccgc atccgccact gatgcctgtt tacccggtag ctcgcggtga
    35221 aagccgtctg tacatgcatc gcatcgaaaa agactggtac acgctgatga acactatcat
    35281 caacggttca gcttctgaag cagatgccgc acgtaagcaa ctgcgcgaag aactgctggc
    35341 gattgcgccg gtcttcggtc agaagccgta cttcctgagc gatgagttca gcctggtcga
    35401 ttgctacctt gctccgctgc tgtggcgtct gccgcaactg ggcatcgagt tcagcggccc
    35461 gggtgcgaaa gagctgaaag gctatatgac ccgcgtcttt gagcgtgact ctttccttgc
    35521 ttctttaact gaagcagaac gtgaaatgcg tctgggccgg agttaatctg tatggatttg
    35581 tcacagctaa caccacgtcg tccctatctg ctgcgtgcat tctatgagtg gttgctggat
    35641 aaccagctca cgccgcacct ggtggtggat gtgacgctcc ctggcgtgca ggttcctatg
    35701 gaatatgcgc gtgacgggca aatcgtactc aacattgcgc cgcgtgctgt cggcaatctg
    35761 gaactggcga atgatgaggt gcgctttaac gcgcgctttg gtggcattcc gcgtcaggtt
    35821 tctgtgccgc tggctgccgt gctggctatc tacgcccgtg aaaatggcgc aggcacgatg
    35881 tttgagcctg aagctgccta cgatgaagat accagcatca tgaatgatga agaggcatcg
    35941 gcagacaacg aaaccgttat gtcggttatt gatggcgaca agccagatca cgatgatgac
    36001 actcatcctg acgatgaacc tccgcagcca ccacgcggtg gtcgaccggc attacgcgtt
    36061 gtgaagtaat acaaaacagg cccaggcggc ctgttttgtc tttttaatga cttaataaaa
    36121 tagcgtgatt gtggcaatca tattcaccac gaaaccgact gccatgggga tcgctgtccg
    36181 cttcaccacc gcaaaaggcg aaacgcctgc aattcctgac gtaacgacca ccaccgcagt
    36241 aatcggcgaa accgcgcgcg ctagcgtcgt ggcaaaatgc atcggcataa tcattacaac
    36301 cgctggtaca tgtagtccgg ctgcgatatt cggaataaga ctggcaaatg acataaacgg
    36361 cgcattgcca gagcccatca caatggcaca aatggcaatg actagcgcca taataatcat
    36421 cacgccaata ccgcccagac cagaatgctc cgcacccctg ataaccgcat caacagtgcc
    36481 aatcgtcgtt aagcctttcg caaatatttc acccgcgacc accagcgtta ccacgttgac
    36541 aaactgcgta cccatgccgt caaaaaacgc ctgcacatcg tccattgttt cgcgcaagtt
    36601 atgcttgcgg aagaactcaa caaacatcgt caccgtcaaa cttagtaaca tcaccaccac
    36661 cagatgcagt tctgacttca tcagcccgac gtgggcgagg aacagcgagc cgagcatcag
    36721 gattaacggc atcacaggta aaatggcgta atagagcggc gggacattat cgagagtttt
    36781 ttgctctgcc tgttcgtgat tgatattttt atcttttttg tcaaaagcgc gttgcacgaa
    36841 aaagtgggag atcgccaccg agataatgac gcaagaggcg accggaagct ggtagtggaa
    36901 gaagtatgtg gcaattttca ttcccgcgac ctgggctgca aaaatggagt tcgtttccag
    36961 aatcccccac tcaatggaca tcgtggttgc gataaccgcc accgcagaga ggcgacttac
    37021 tcccagactc accagcgtcg gaaataaggt gaccatcagc aa
//
LOCUS       DAJVPP010000031        36687 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVPP010000031 DAJVPP010000000
VERSION     DAJVPP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.031"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(164..922)
                     /gene="deoR"
                     /locus_tag="OQM34_001538"
     CDS             complement(164..922)
                     /gene="deoR"
                     /locus_tag="OQM34_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS1452035.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(980..1576)
                     /gene="ybjG"
                     /locus_tag="OQM34_001539"
     CDS             complement(980..1576)
                     /gene="ybjG"
                     /locus_tag="OQM34_001539"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1452036.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1861..3087
                     /gene="mdfA"
                     /locus_tag="OQM34_001540"
     CDS             1861..3087
                     /gene="mdfA"
                     /locus_tag="OQM34_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCS1452037.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3128..3412)
                     /locus_tag="OQM34_001541"
     CDS             complement(3128..3412)
                     /locus_tag="OQM34_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452038.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3498..4313)
                     /gene="ybjI"
                     /locus_tag="OQM34_001542"
     CDS             complement(3498..4313)
                     /gene="ybjI"
                     /locus_tag="OQM34_001542"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1452039.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4313..5521)
                     /locus_tag="OQM34_001543"
     CDS             complement(4313..5521)
                     /locus_tag="OQM34_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1452040.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5605..6141
                     /gene="rcdA"
                     /locus_tag="OQM34_001544"
     CDS             5605..6141
                     /gene="rcdA"
                     /locus_tag="OQM34_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1452041.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6316..8001)
                     /locus_tag="OQM34_001545"
     CDS             complement(6316..8001)
                     /locus_tag="OQM34_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1452042.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8271..8648
                     /locus_tag="OQM34_001546"
     CDS             8271..8648
                     /locus_tag="OQM34_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1452043.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8678..8935)
                     /gene="grxA"
                     /locus_tag="OQM34_001547"
     CDS             complement(8678..8935)
                     /gene="grxA"
                     /locus_tag="OQM34_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1452044.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9095..9382
                     /locus_tag="OQM34_001548"
     CDS             9095..9382
                     /locus_tag="OQM34_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1452045.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9366..10088
                     /gene="nfsA"
                     /locus_tag="OQM34_001549"
     CDS             9366..10088
                     /gene="nfsA"
                     /locus_tag="OQM34_001549"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS1452046.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPGLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDFLHKQGWATR"
     gene            10149..11051
                     /gene="rimK"
                     /locus_tag="OQM34_001550"
     CDS             10149..11051
                     /gene="rimK"
                     /locus_tag="OQM34_001550"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1452047.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11139..11615
                     /locus_tag="OQM34_001551"
     CDS             11139..11615
                     /locus_tag="OQM34_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1452048.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11966..13078
                     /gene="potF"
                     /locus_tag="OQM34_001552"
     CDS             11966..13078
                     /gene="potF"
                     /locus_tag="OQM34_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1452049.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13173..14306
                     /gene="potG"
                     /locus_tag="OQM34_001553"
     CDS             13173..14306
                     /gene="potG"
                     /locus_tag="OQM34_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1452050.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14316..15269
                     /gene="potH"
                     /locus_tag="OQM34_001554"
     CDS             14316..15269
                     /gene="potH"
                     /locus_tag="OQM34_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1452051.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15266..16111
                     /gene="potI"
                     /locus_tag="OQM34_001555"
     CDS             15266..16111
                     /gene="potI"
                     /locus_tag="OQM34_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1452052.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16171..16659
                     /locus_tag="OQM34_001556"
     CDS             16171..16659
                     /locus_tag="OQM34_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1452053.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16700..17827
                     /gene="rlmC"
                     /locus_tag="OQM34_001557"
     CDS             16700..17827
                     /gene="rlmC"
                     /locus_tag="OQM34_001557"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1452054.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18002..18733)
                     /gene="artJ"
                     /locus_tag="OQM34_001558"
     CDS             complement(18002..18733)
                     /gene="artJ"
                     /locus_tag="OQM34_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1452055.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19025..19693)
                     /gene="artM"
                     /locus_tag="OQM34_001559"
     CDS             complement(19025..19693)
                     /gene="artM"
                     /locus_tag="OQM34_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1452056.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19693..20409)
                     /gene="artQ"
                     /locus_tag="OQM34_001560"
     CDS             complement(19693..20409)
                     /gene="artQ"
                     /locus_tag="OQM34_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1452057.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20416..21147)
                     /gene="artJ"
                     /locus_tag="OQM34_001561"
     CDS             complement(20416..21147)
                     /gene="artJ"
                     /locus_tag="OQM34_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1452058.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21165..21893)
                     /gene="artP"
                     /locus_tag="OQM34_001562"
     CDS             complement(21165..21893)
                     /gene="artP"
                     /locus_tag="OQM34_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1452059.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22111..22626)
                     /locus_tag="OQM34_001563"
     CDS             complement(22111..22626)
                     /locus_tag="OQM34_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1452060.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23275..25044
                     /locus_tag="OQM34_001564"
     CDS             23275..25044
                     /locus_tag="OQM34_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1452061.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25255..25578
                     /locus_tag="OQM34_001565"
     CDS             25255..25578
                     /locus_tag="OQM34_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1452062.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25575..26405
                     /gene="amiD"
                     /locus_tag="OQM34_001566"
     CDS             25575..26405
                     /gene="amiD"
                     /locus_tag="OQM34_001566"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1452063.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26402..27415)
                     /locus_tag="OQM34_001567"
     CDS             complement(26402..27415)
                     /locus_tag="OQM34_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1452064.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27514..28944)
                     /locus_tag="OQM34_001568"
     CDS             complement(27514..28944)
                     /locus_tag="OQM34_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1452065.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28955..29956)
                     /gene="ltaE"
                     /locus_tag="OQM34_001569"
     CDS             complement(28955..29956)
                     /gene="ltaE"
                     /locus_tag="OQM34_001569"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1452066.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29993..31711)
                     /gene="poxB"
                     /locus_tag="OQM34_001570"
     CDS             complement(29993..31711)
                     /gene="poxB"
                     /locus_tag="OQM34_001570"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1452067.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31844..32812)
                     /gene="hcr"
                     /locus_tag="OQM34_001571"
     CDS             complement(31844..32812)
                     /gene="hcr"
                     /locus_tag="OQM34_001571"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1452068.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32824..34476)
                     /gene="hcp"
                     /locus_tag="OQM34_001572"
                     /gene_synonym="priS"
     CDS             complement(32824..34476)
                     /gene="hcp"
                     /locus_tag="OQM34_001572"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1452069.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34620..35519)
                     /gene="lysO"
                     /locus_tag="OQM34_001573"
     CDS             complement(34620..35519)
                     /gene="lysO"
                     /locus_tag="OQM34_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1452070.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(35976..36671)
                     /gene="aqpZ"
                     /locus_tag="OQM34_001574"
     CDS             complement(35976..36671)
                     /gene="aqpZ"
                     /locus_tag="OQM34_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCS1452071.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9171 a         9118 c         9405 g         8993 t
ORIGIN      
        1 attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt ctttggtagg
       61 atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt ctcttaatct
      121 tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac atcaacttaa
      181 tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca ctcaccacaa
      241 tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat ttactgtggt
      301 cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc ggcaactctt
      361 caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa taaaaagcga
      421 tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa atggcgttgc
      481 tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct ttcagcgcca
      541 gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca atcgcttcaa
      601 taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt tctaccagtg
      661 tcgcagccag ttttgccgcc cggcgttttt cttccaccaa gcgggatttt tgatcgctta
      721 acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc aaaacgacgg
      781 gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca agcagggcgg
      841 cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc tgcccgatac
      901 gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag ccgctcaata
      961 ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt aatgcaaaac
     1021 agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct tgccagataa
     1081 tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata tccagcggcc
     1141 agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc attaaaagtg
     1201 agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa atcaccgtac
     1261 cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag ccgatatttt
     1321 cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca aacaggctga
     1381 ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc ccccacaacc
     1441 aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata aaaatcgcca
     1501 acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat agagagagat
     1561 tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca atacaggcaa
     1621 gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta tttccctcaa
     1681 gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact taaagaaagc
     1741 gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct acaaaattcg
     1801 ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg aagaaattgc
     1861 atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact tttccctctc
     1921 tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca acccggtatg
     1981 ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc gatgaccgcg
     2041 tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga tcgtattggt
     2101 cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct ggcaatatta
     2161 ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat aagcttctgt
     2221 ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc ggtttgtatc
     2281 aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg tccgctggtg
     2341 ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt tgccgcattg
     2401 gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat gcgtataggc
     2461 gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa gaacggccgc
     2521 tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat cgcccagtcg
     2581 ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt gctgcaagtg
     2641 cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac ctcgcgccgc
     2701 accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct gttggtcgct
     2761 gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg gttaagtatt
     2821 tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct gtttgccagc
     2881 gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct gatctttacc
     2941 gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact gtttaatctc
     3001 ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt aaaagataaa
     3061 cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc gatcaggcat
     3121 tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct tcacctacgc
     3181 tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca ggagccacat
     3241 agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact gaatcaggaa
     3301 ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc atcgccacac
     3361 cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc atttcgcctg
     3421 aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc attgtgttgc
     3481 cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac tttatcgatc
     3541 acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc tgcgacgact
     3601 gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat ctcaatatcg
     3661 ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa tttctgcagt
     3721 tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct gccgttgccg
     3781 gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg tacttgtgga
     3841 atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa gttgtcaaag
     3901 ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat ggcatcgtca
     3961 tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac ttccgggcgc
     4021 gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc attaaaaaca
     4081 tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat ttcattcgca
     4141 atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac cacaaagcga
     4201 atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt ataggttttt
     4261 tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct catcaactat
     4321 tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg acaatagccg
     4381 cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa tgttcgccga
     4441 gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt gctaccacac
     4501 tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc agcgggaagc
     4561 ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag gcgctatcga
     4621 caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga accacggcaa
     4681 cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc atccccaggg
     4741 taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca tcaaccatta
     4801 ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc actacaacac
     4861 caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca tgctgggtgc
     4921 catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata taaataggcg
     4981 cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca aaggccgtca
     5041 gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg tgcatcatcg
     5101 gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt atcgccactt
     5161 ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc aggggcgatg
     5221 tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac atcgtgacca
     5281 ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga ataccgctca
     5341 tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag acagagagaa
     5401 tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct ggcaaaaaga
     5461 agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa tttacggtca
     5521 tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt acatttgtac
     5581 acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg gcgagaaaaa
     5641 attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt tacgcaccgc
     5701 aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt ttcaggaatt
     5761 gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg gcaatatcag
     5821 gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac cgatatgatc
     5881 tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct ctacgcgctg
     5941 gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg cagtcagcaa
     6001 acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt tattgagggg
     6061 atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat tttgaggatg
     6121 gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca tcaaccttga
     6181 accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa agcagtggca
     6241 ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa tttcattttc
     6301 agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg tccctgccag
     6361 cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag ggatgttact
     6421 gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc ccatcattgc
     6481 gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga aacagataac
     6541 cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa tcgcgcccag
     6601 gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca ccatcaagcc
     6661 gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg ggtggttagc
     6721 acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc ccatgccgaa
     6781 actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa caaagaaggc
     6841 gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga tgcgatcggc
     6901 gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt tgttaagcac
     6961 gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa ggaagcaacc
     7021 gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt ttttaacgac
     7081 cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt ctttaccgtt
     7141 acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg ctatttcatc
     7201 gcccatttgt agcacggcat caccgtctgg atttgccaga attccgttac gtcgaatacg
     7261 ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt tgccgtcggt
     7321 ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat aaaccttacg
     7381 gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg tctgtaagtc
     7441 ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca gaccgattaa
     7501 ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct gcctgctttc
     7561 catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg acgtcataga
     7621 gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac ctaaccctaa
     7681 ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat ttttcccatc
     7741 acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga aaataaacag
     7801 cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc ctaataacag
     7861 cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc gtaactttcc
     7921 gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc catttaacaa
     7981 ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc tgttgaaaga
     8041 aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca ttcagcgcac
     8101 ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg tatcaacggc
     8161 tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat cttactgaac
     8221 gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga gtgaaacata
     8281 aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg tgcctttttc
     8341 tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc ggcttgctgt
     8401 ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa gtcctgaaac
     8461 ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg ctgttttttt
     8521 caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg ttctggtgtg
     8581 cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag cacagaaaaa
     8641 atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga ttttctttca
     8701 cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa atctgcggca
     8761 cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc ccttccgcac
     8821 gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc tcagccagat
     8881 cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt tgcattattt
     8941 ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct gaaagtaggt
     9001 ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca gtgaaaagtt
     9061 ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta aattgcctaa
     9121 aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc tgctgtcggt
     9181 aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga ttctgatgat
     9241 ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc aggtggcaaa
     9301 acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag aaagagaaaa
     9361 agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc catttcactg
     9421 atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt gcgacgtcca
     9481 gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca ttacgtgaag
     9541 aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag ttctgggtgt
     9601 tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc ggcctggcgg
     9661 aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca ttaaccgcag
     9721 cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat attgaagcgg
     9781 tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg tgccttggct
     9841 ggcctgcgga taatccggat ctcaagccgg gtttaccggc ctccattttg gtgcatgaaa
     9901 acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa ctggcggaat
     9961 attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat atccgccgaa
    10021 caatcattaa agaaagccgc ccatttattc tggatttttt gcacaaacag ggttgggcga
    10081 cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt ctgacagaga
    10141 ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg tgtaagcggc
    10201 tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg ctttcttgct
    10261 acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag ttaccccatt
    10321 ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg gcggcactgc
    10381 gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt gcccgggcgc
    10441 gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg cctgtcacgg
    10501 gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt ggtgcgccgc
    10561 tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg gagactcgtc
    10621 aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt ctggtgcagg
    10681 aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt gatgaagtgg
    10741 tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg catcgtggcg
    10801 gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata aaagccgcgc
    10861 gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc gggccgttgg
    10921 tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc ggtatcgata
    10981 tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat tgcctgaaaa
    11041 cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt ttgcgtaagc
    11101 tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg gtcgttcctg
    11161 gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt gaatgtgata
    11221 actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt gatgccaaaa
    11281 tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga ccttcagccg
    11341 tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc gtgaaagcat
    11401 ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct ttatccgtta
    11461 tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa gagcagattt
    11521 cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac gatgaagggc
    11581 aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc acgcagtcgc
    11641 tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc ttaaagatta
    11701 tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca taaaaatttg
    11761 ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt tattcttgcg
    11821 atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt acaccgcgcc
    11881 gataaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg ttcgtttgtt
    11941 aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc tatcgggtct
    12001 ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac aaaaaacact
    12061 ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt ttgaaaaaga
    12121 aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg aaggcaaatt
    12181 aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc tagaacgcca
    12241 gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt ggaagaatct
    12301 cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat ttgctatgcc
    12361 ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag ctgtgctggg
    12421 cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc tggaaaaact
    12481 gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta ccgtgttgaa
    12541 ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac cggcaaccga
    12601 tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat acattaacga
    12661 cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct ggcaggcgtc
    12721 aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc caaaagaagg
    12781 ggcgatggcg ttctttgatg tattcgccat gcctgcggat gccaaaaaca aagacgaagc
    12841 ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt ccgaccatgt
    12901 gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag tccgtgagaa
    12961 cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga aagtgcagga
    13021 cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg gaaaataatc
    13081 cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac catcatggtg
    13141 cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct cgcccgcagg
    13201 cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc aaatcctacg
    13261 atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa atcttcgcgc
    13321 tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca ggtttcgaac
    13381 aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt ccgccttacc
    13441 tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg accgtggaac
    13501 agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt tccagtcggg
    13561 tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa ccgcatcagc
    13621 tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag cgcccgaaac
    13681 tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac cggatgcagc
    13741 ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc acccacgatc
    13801 aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg aaatttgtcc
    13861 agattggcga accggaagag atctacgagc atccgactac ccgctatagc gccgaattta
    13921 ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat ggcctggtgc
    13981 ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg gtggtcgata
    14041 acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa gagccgcccg
    14101 ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc ggcgatcttt
    14161 cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta caaaacgccc
    14221 atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc tgggaagtgg
    14281 acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa cctgctgccc
    14341 agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg aagcatgggc
    14401 gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg ctgccatttc
    14461 tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct tataccgaac
    14521 tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat tttctgcaac
    14581 tgaccgacga ttcgctctat ttcgatgctt atctccagtc gctacaggtg gctgcgattt
    14641 cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg cacagtaagc
    14701 cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc tcgtttctga
    14761 tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat aattttctgc
    14821 tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg gcagtttata
    14881 ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc gcgttgattc
    14941 gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg ctgaaaacgt
    15001 tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg atgctggtgt
    15061 ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg gacagcatca
    15121 tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg gtggcctcgg
    15181 cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc cacaaacacc
    15241 agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt cgccctggcg
    15301 gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga tgctggttat
    15361 ctattcgttt aacagctcga agctggtgac ggtgtgggcc ggctggtcaa cgcgctggta
    15421 tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc tgacaattgc
    15481 tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg tgctggtgcg
    15541 ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc cgctggtaat
    15601 gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg ctcatgccat
    15661 tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca ctttctgtac
    15721 ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct cgatagaaga
    15781 agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta cgctaccgat
    15841 gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc ttgatgatct
    15901 ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc tggtcttttc
    15961 cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga tcctcggcgc
    16021 ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa aacagcggat
    16081 acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa cctggctaca
    16141 taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg attttttaag
    16201 aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt ggcggcgctc
    16261 gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct gggcgtgcgc
    16321 ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct ggtcttttta
    16381 agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt gaaagggcgt
    16441 cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta cttgtgggcg
    16501 gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc aaaacgtgaa
    16561 atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat gctgctgttc
    16621 gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc gtcgcccctg
    16681 atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct gtcgttcctg
    16741 tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg atcttaaaaa
    16801 tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc cggaacaagg
    16861 gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac tgctcggtat
    16921 gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc cagcctcatt
    16981 tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa cgccctacaa
    17041 cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc agagtgatgg
    17101 cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac tgcgtaaggc
    17161 gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca atattcagcc
    17221 ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac aacaggcact
    17281 ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct tccagactaa
    17341 tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac agctgccggt
    17401 taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact gcgcgacgcc
    17461 tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg caaagcagtc
    17521 agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca ctcagtttgc
    17581 cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc gcggcattgg
    17641 taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct actccagctg
    17701 taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca tcgaacgggt
    17761 acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc tgctggtgaa
    17821 gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt tgttcatgcc
    17881 ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat acattgcaga
    17941 gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc atcagtctca
    18001 attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct ttaattgctg
    18061 ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca gcgataccca
    18121 ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc agttgcggat
    18181 tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca atacgaccat
    18241 ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc acttccgggt
    18301 gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg cgtttgcctt
    18361 tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct gagttttcat
    18421 agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc ataccggaga
    18481 ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac gcgtgattag
    18541 taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg atatcaaagc
    18601 cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct gaaacgccaa
    18661 aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa gcggcaagaa
    18721 ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc cgtgtctgcg
    18781 gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt aaataattta
    18841 aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat aagaatttat
    18901 gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc gtgctgatgt
    18961 ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa tgtacgcagc
    19021 tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc atcatcagcg
    19081 tcagcaggcc gttgacgacc aggtaaataa tccctgccgc accgaacacc attacatcgt
    19141 aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta atggtgtaag
    19201 ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag agcgagcgtt
    19261 taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc agggcgctac
    19321 aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc tgcgtggtat
    19381 acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct gacaacaaat
    19441 gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa taaatcagga
    19501 agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc accagccaca
    19561 ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga atcagtgcca
    19621 caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc agctcgggta
    19681 aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt caatgcgttt
    19741 gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca caatgtacca
    19801 ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta aatcattcac
    19861 actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt taccgaggcc
    19921 aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag ccgatttcga
    19981 cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca acgcgccgcg
    20041 cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac cacaaaggaa
    20101 cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga acccaagatt
    20161 gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat agataaacag
    20221 cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac ctgcccacgc
    20281 gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg ccagcccgac
    20341 aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta aaggaaaaaa
    20401 ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt aagtgccatc
    20461 tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt tgccctgacg
    20521 taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat cgcccactgc
    20581 cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac caaatacgct
    20641 gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat acggaacggt
    20701 ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt tctgaacgcc
    20761 gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac ccacaaacag
    20821 ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct ccggagtgat
    20881 atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga tcaggctgtc
    20941 aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg cctgtgccag
    21001 gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg gaggataggg
    21061 ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa aacctgcaat
    21121 taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg agagagatag
    21181 tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg ttctacgata
    21241 tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc aacttcgtgg
    21301 gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct gacgatttgt
    21361 gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac ctgcggttcc
    21421 atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag atgcagcggg
    21481 taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc acgggccagc
    21541 gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt ttgctgcacg
    21601 gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg acgcaaatcg
    21661 cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc aatgttgagc
    21721 gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct tttacccgcg
    21781 ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt gatatcgaac
    21841 agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact cattgacact
    21901 cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttgac agaataatta
    21961 tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa catagcacaa
    22021 aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa atgccggata
    22081 atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact gacggagcgt
    22141 acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga cccaacactg
    22201 gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt tgagatccac
    22261 ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg caacgtgggc
    22321 gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt cacgatgact
    22381 attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat aggggcgcag
    22441 ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt gagccacact
    22501 gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat aagctggcgt
    22561 gacggtggta caggcactca gcagcagtgc acatggaata agtagagata gtgaacggta
    22621 gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca aacgctatca
    22681 gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc tgattgggtt
    22741 tgagtccatt gctggctacc cactcaaggt tgctcgtagc tgacttactt aaattaccca
    22801 aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg aaatgatcat
    22861 ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga ggttaagagc
    22921 atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg ttattgtcta
    22981 atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga ttgccaaaat
    23041 tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact ctcgataata
    23101 ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt gaagaataaa
    23161 ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt gcttttataa
    23221 ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt taatatgata
    23281 aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca aagtgataat
    23341 ttcctggatt cactgaggga atttttcgcg atattaaatt ccagtcgtaa gggtgagttg
    23401 tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga cagtgatctc
    23461 ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca atttaataca
    23521 ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc agaagataac
    23581 gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa tgtatcgaat
    23641 ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa attacaagaa
    23701 caattagata agtattttac gattgaatcg cacccattat tatacaggta taccattggt
    23761 agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt taacaaagaa
    23821 caatatatat cctttataaa ggacgcattt attcattttt atgattatag caggcgatat
    23881 gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt gatgagcact
    23941 ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac agaagaagag
    24001 tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc tgatgataat
    24061 tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc ctttcaagat
    24121 accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat ttgtagcgta
    24181 agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa tgaagaagtt
    24241 ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag taatttagtc
    24301 aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt taatgacgtg
    24361 gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt gaatgaaagc
    24421 atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat aacaaatagc
    24481 aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat taataacgta
    24541 aatactcatt ctttaaaatt tattaactgc aattttttca atgttgatat gataagagtt
    24601 aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat aaaaccctgg
    24661 ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg tttactatct
    24721 ctgtactcta tctgtgcaag ggatatatat aatatgaacg ccgggaataa cgtgaaggtt
    24781 gctgcggatg ctttccttga aattattttc tctcttaaaa ataaatattg tattaaacta
    24841 ttaagcgctc aggatcgggc gtttatatac gagtttgcaa gaatgatctt cgcttatatt
    24901 aatgacaaaa gcattgagat cttattactt tcttgttttg cggcggctga ccaaaaagca
    24961 atccagcggt atagacctca gtcacaagat ggggaagact ttaggagtca tttacaatat
    25021 aagttgcctc tttccgctca ttaatgttgt tggtgcaaca taataagaca ggtaagcggt
    25081 accctataat gaaaccaata tcgggttatg caacggataa tcgtaaagac ttaagtgggt
    25141 gtttttgaca caatgagatg atttcctaaa ctgatcaaaa ccttcttccc aacatggcat
    25201 atcagtccga taatgcttta gagatcgaag gcttaacaaa caaggagatc gatcatgcaa
    25261 ttttctacaa ccccaactct ggaaggcctg accatcgttg aatattgcgg tgtggtgacc
    25321 ggcgaagcga ttttaggtgc caatattttc cgtgatttct ttgccggtat ccgcgatatc
    25381 gttggcggac gttccggtgc gtatgaaaaa gaactgcgta aagcacggga gatcgccttt
    25441 gaggaattag gctcccaggc gcgggcgctg ggggccgatg ccgtagttgg tattgatatc
    25501 gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag cggtacggcg
    25561 gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg ttactggcag
    25621 ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat acccgacacc
    25681 aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc gcagatgatt
    25741 ttgatagctc gctggcgaca ctgactgata agcaggtcag ctcgcattat ctgatccctg
    25801 cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg gaacaagaac
    25861 tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgccttaac gacacctcta
    25921 ttggcattga gctggaaaac cgtggctggc aaaaatcatc cggagtgaaa tattttgccc
    25981 cgtttgaaca ggcacagatt caggcgctta ttccgctggc gaaagatatt attgcccgtt
    26041 atcacatcaa gccggaaaac gtagtggcac atgcggatat cgcaccgcag cgcaaagacg
    26101 atccggggcc attatttccc tggcagcaac tggcgcagca ggggattggt gcctggccgg
    26161 atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg gtagataccg
    26221 cgtcattgct ggagcttttg gcgcgctacg gttatgacgt aaaacctgat atgacaccgc
    26281 gcgagcagcg gcgcgtgatt atggcattcc agatgcattt ccgcccgacg ttatataacg
    26341 gcgaagcgga tgcagaaact caggcgattg ccgaagcatt gctggagaaa tacgggcagg
    26401 attagcgcgg cagttttccg tggtcgcgta gccaggcggc agttttctcg atgccttcat
    26461 ccagggtgat gaccggctga taacctaact cttcctgcgc acgcgtaata tccagcgtaa
    26521 agtcaaaatt caacttggag acgccgtagt gggtcagcgg cggctctttt gctgacttgc
    26581 ggcctaaacg ctccatgctg cgggcgatca tatccagcat cgggtaaggg acggaacgaa
    26641 tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc agtgtgcgat
    26701 gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag gcttcctggc
    26761 ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc agcgcactgc
    26821 cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga cggggaataa
    26881 agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag cgcgtttgtg
    26941 gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg cgggcaaact
    27001 cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatt gcgatggtgg tgataatcga
    27061 agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg acagcccatt
    27121 cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt gtcccccagg
    27181 gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc actttagctt
    27241 gtgatgaaac cagctcggtc agatccgtcg gaacaaactc tgcgcccatt ttttccagca
    27301 atttacccat tgcttcgttg cgaccgctcg cacgcacgct gatgcctttc tggcataaaa
    27361 actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc ttcatatcaa
    27421 tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccca tgatgcaatg
    27481 ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt ctgccagtct
    27541 ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca tcaataacca
    27601 gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga tagtacgata
    27661 gtcaccttta tcttccaggg taaaacacag tcgtcccagc cccggcgctt tcatgccaaa
    27721 taacaacgta agttgctttt tcggttcaac gacaatcact ttccagctat ccaccgcatc
    27781 gccggtctgt aaatattcgc gctccgggcg gcctttcgcc agattatgac cgatcgcgcg
    27841 gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct ctttaccgcc
    27901 gatttggttc accaccagcc ataaagccgc aaggctggcg gacgttttaa cggtaaaccc
    27961 cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg gcaaaggcct gagcgtcgta
    28021 gccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac gtaccgcgtc
    28081 atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat ctgccagcag
    28141 atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg aagtaatcac
    28201 attgagaaac cacaccgaaa tccagcgggt ggggaggggg atggggatca accagcggcg
    28261 cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca cctctggtcc
    28321 ggcggcttcg aagatgcggt gttcgttggc tggatggtct aacagcgcca ccagatagtg
    28381 cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg gcggcgttaa
    28441 cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac ctgcgccaac
    28501 gataattccg gcccgaagtt cggtcactgg tacattcgct tcacgaagaa tgtccgccgt
    28561 agcctgacga gctcgcagat gatccgactg ctcatgtggc ggggcctgca acgaactgag
    28621 aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga gagccagctg
    28681 gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa aatagacggt
    28741 atcgatatcc tgcaacaggg ccggaaggtt atccggccag ctgagatcga ctttatggca
    28801 actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg ccgctaggat
    28861 ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc cactggcacc
    28921 gagaactaaa atgcgttgcg gcacgtatct ctccttaacg cgccaggaat gcgcgccagt
    28981 gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc aggcggacaa
    29041 tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac gcggcagcat
    29101 tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca tccgcgcctg
    29161 cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc aaccgtgcga
    29221 cgttattttt cagggcatac atcccggcgg cagccagaat cccggactga cgcatcccgc
    29281 caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta ccgacgagta
    29341 atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa tcacaatatt
    29401 gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg cgcgcaccgt
    29461 caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc aggtattcac
    29521 gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg cgggcgaaat
    29581 ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg ccgtcggcag
    29641 ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg gcttcaaaca
    29701 gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag tgactgagca
    29761 gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct ttaccggaaa
    29821 gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa acgtcgtccc
    29881 caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta acggtatcac
    29941 tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct ttttacctta
    30001 gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgata attgcgcgca
    30061 acatatacag gctaaaacct ttggcctgtt cgagtttgat ctgcggtgga atggctaatt
    30121 cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatagagaag gcgcgttgca
    30181 gggcttcatc aacttcagag gctttttcta cacggatacc cgtaatgccg cacgcttcgg
    30241 caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtaa cctccggcct
    30301 tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt ttcaccggca
    30361 gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg ccatcgccgc
    30421 acatggcgac cacctgacgt tccggctcgg ttgcctgcgc acccaacgcc tgcggcatgg
    30481 cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg ttcattttca
    30541 gataacgtgc cgcccatacc gttggcgtgc cgacgtcaca ggtaaagatg gcgtcatcgg
    30601 cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc tcgctcggtt
    30661 tagctaaatc gtccagccct ttgcgggcat cgcggtaatc ttccagcgct ttatccagaa
    30721 acttgcgatc ggttttttct tccaccagtg gcagcaatgc gcgcagggtt gatttgatat
    30781 cgccgaccag cgccatatct accttgctat gcgcgccgat gctggctgga ttaatatcaa
    30841 tctgaataat tttggcatcg gtcgggtaga acgcacggta ggggaactga gtaccgagta
    30901 gcactaacgt gtcggcgttc atcatggtat ggaaacctga cgagaatccg attaacccgg
    30961 tcattccaac atcatacgga ttatcgtatt cgacatgctc tttaccgcgt agggcatgaa
    31021 caataggtgc tttaattttc ccggcaaact caactaactc tttatgcgcc cccgcgcagc
    31081 cgctgccaca catcagggcg atattgctgg aataacgcag cagttgcgcc agtttgcgta
    31141 actcttcttc ttccggcatc acgactggct gtggcgcatg ataccagtgc gtggttgccc
    31201 cttctggcgc aggttttaac gccacgtcgc ccggtaatac aacaacggaa acgccacggt
    31261 taagcaccgc tttgcgcata gcaattgcca gcacttgtgg gatctgctcc gggctggaaa
    31321 ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt tcctggaaat
    31381 agccgctgcc aatttcgctg gagggaatat gagcggcaat cgccagtacc ggaacgttat
    31441 tgcggtggca atcgaatagg ccgttgatca agtgcaggtt gcctgggccg cacgatccgg
    31501 cacagaccgc cagttctcct gaaagttgtg cttcagcgcc agcggcaaac gccgccactt
    31561 cttcatggcg ggtggacatc cactcgatgg tgcccatgcg attaagactg tcgctaagac
    31621 cgttcagaga gtcgcccgtg actccccaga tgcgtttcac gcctgccgat tcgagtgttt
    31681 tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat gtgataacgg
    31741 taacaagttt agttcatctg acggaggggg aagggatggg agagaaagga ggcactaacg
    31801 gttaaatagc ccgatgaaag gaatatcatc gggcataagg cgattatgcg agaaccaaat
    31861 ccccctgcgg atggcaggag caggccagta cgtaaccttc agcgatttcg gcgtcggtca
    31921 gcgtcattgt actgctcacc gtatattcac cggaaaccac ttttgtctta cagcagccgc
    31981 aaacacccgc acggcaggca gcgacaaccg gaacgttatt gctttccagc gcctccagta
    32041 gcgtggtgcc aaccggggcg taaaattctc gtgccggttg cagtttggtg aatttcagac
    32101 cgctggtcgc cgcttctgct actggggtga agaatttctc tttaaagaaa cgcgtcacgc
    32161 cgagcgcttt cacttcctgc tctacccaat ccatatacgg agccgggccg caggtcatca
    32221 cggtacgtga agctaagtca ggtacatctg ccagcagttc gcgagtgaga cgaccagcga
    32281 taaagccttc ggtaacgtta ttttctgcca ccagcgttac cggatagtta cgccactcat
    32341 cggcgaaaat aacatcctgc ggcgtacgca cgttgtagat cacccgcaca tcggcctgtg
    32401 gacggttctt cgcaagccag cgacgcatcg acataatcgg cgtgacgccg cagcctgccg
    32461 ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc atcgcgtccg
    32521 aaagccagag ataatcaccg cgttttacat cgcgcgtcag ccactgggag ccgacaccgt
    32581 catcaatccg ccgcacggtc agggtgatat attcactcac gcctggcgtg gaggaaatgg
    32641 tgtaagcacg cagcgtttcc gctgagtttc gcacgctgac cagtgcatat tgcccggcgc
    32701 gatatgggta gtgatcgtgg caaatcaggg aaatcgtcca cacatccggc gtttcttgcg
    32761 taatgtgatg aacctgtatc cgccacgggc attgattcgt tggcatcgtc atcgacaaac
    32821 tccttacgcg ttcaacagtt gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc
    32881 gaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg aaaccaggtg cagtcgggcc
    32941 ggtgacgata tttttcacgc ccagagaaag cagcgtcagc agaatgacaa tcgctttctg
    33001 ttcaaaccag gagagcacca gcgacagcgg cagatcgttc acaccacagc ccagtttctc
    33061 tgccagagtg acagccagaa taattgctga gtaagcatcg ttacattgac ctgcatctac
    33121 cagacgcggc agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat atttaccaca
    33181 ggcgagggtc aggatcaggc agtcatccgg cacgctggtg gcgaaatcgg tgaagtagtg
    33241 gcgctcgccg cgtgcgccgt cacagccacc aagcaggaag atatgacgca gtttttcacg
    33301 gctaaccaga tcaatcagcg tatcggctgc accaagcagc gtctggcgac caaagcccac
    33361 ggtgatcagg tgaggaattt cgctgtacgg gaagcccgcc atttgttgcg cctgggcgat
    33421 gaccgcagag aaatcttcgc catccagatg acgcacgcca ggccagccaa cgatgctgcg
    33481 ggtccagata cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat
    33541 cacgatgggg ccagggaaac gagcgaactc cacttgctga ttctgccagc cgctgccgta
    33601 gttaccgacc agatgcttga atttacgcag ctccgggtag ccatgcgcag gcagcatttc
    33661 gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc
    33721 tttgagatcg tgaccggaaa tcagaatgca tttacccgcc gtcgctttga cgttgacctg
    33781 ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca tccagaatgc tcatcacttt
    33841 gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat cggcaggcca
    33901 ggtccccagc cacgccatga ttttatggta ctgggcataa atatcgttgt cgtattgacc
    33961 gagaacatgc gcgtgttcca tataggccgc cgcacctttc aggccataca ggcacagcag
    34021 acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg taaattctgc
    34081 tgcctgacgt tgcagctcgc cgagatcatc gctcaccagt tgcaggtcag ccatcgggtt
    34141 atcgacgcgc gcgttggcat ctacagccag gcattgtgct ttcagcgcct cgcgcaaggc
    34201 aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt tggtcagggt
    34261 tgagaaaaag gcacgtggcg cgaagctgtc aacatcgtgg ttgatgatgc cgtattcacg
    34321 cgcttttacc gcccaggcag aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc
    34381 agaagtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt tttctgccgg
    34441 agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta aagttatatt
    34501 taatatacat gtttaagggt aagacgctta acgcggggat aaaagggatt tttcatgcaa
    34561 ctttaaggga gattgattta gcgcaatttt ggcggcaggg aactaccgcc agagaggtat
    34621 tacgcagaga aaaaggcgat gaggatcggc actaacaggc taagaataaa accgtgaaca
    34681 attgccgccg ggaccatatc cagcccgcca gtacgttgaa gaacgggcag ggtgaaatcc
    34741 attgatgtgg caccgcataa gcccagtgca gtagagcggc tgcggcgaat cagcccaggg
    34801 atcaacataa tagcaatcag ttcacgggcc agatcattaa aaaacgccgc gctcccgatt
    34861 accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc gaaaccggag
    34921 gccattgcca gcgcggtatt gatggggaga tcaagaataa aggcgttaat taaaccacca
    34981 attaatgaac tgacaaccac caccacggcg acaatcattc cccggcgatt aaggacaatc
    35041 tgctttaagg tcatgccatt attgcgcaac tgaataccaa cgaggaaaag tagcaaaatt
    35101 aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact cagaccaatg
    35161 gcaaaaccaa tcactactac gccgcacagt tttagcgact ccagcgccat cgcaatacgc
    35221 gacgggagtt tttcttgctg atggtggttg cgccacggca ggcctcgctc cagccacatc
    35281 agggcggcaa tattacacag taaaataacg gtaatactga cggcagaata atgcagaatc
    35341 gccaacaggt tactggcgag gttatcgaga aacgccagac tgatacccat aaaaaagaga
    35401 ataaggtaaa ccatccagct taatagctga ttaatagctt ttaacgcagc ttgttggcga
    35461 agcggaatga ggtaacccac aatcagggga accagaatga ttaacagccc agaaaacatg
    35521 aaaatccagt ccttgcaaag aagaagtcga aatgcgcgat gacacactac tgaaagcgga
    35581 agggcgagta aagttgcaat taaaaggaaa tgttatgcat cagaggggga tttttaatcc
    35641 tatctgagtg tttagcattg ttgcgctcct ttcatgtgca atgccatata aagccactgg
    35701 cggagggagc gcgcaggggg cagccagtcg ccgccgcccc ctgcaccccc gggctctggc
    35761 gaacaaaatc gccgctgcgc ggtaccttca gcttatcccc ttcggcaacc gggtcgggcg
    35821 cgaggtaacg tcctgtaaga cgcgccctca gcccacatcc atgtgggctg ccccggcctt
    35881 cggggaacgc ttcagcgatt ttgacgccac caatacccgt gcggtttgta atgtcgaaga
    35941 taaaacaact agggcagata aaatgccaat gctaattaat cacgcttttc cagcagagtc
    36001 cggtaaatca gaccaccgat aatgccgccg acaattggca ccacccagaa gaaccacagt
    36061 tgttctaatg cccagccgcc ctggaagata gcaaccgcgg tgctgcgcgc cgggttaaca
    36121 gaagtgttag tcaccggaat actgattaag tgaatcaggg ttaaggccag accaatagcg
    36181 atcggcgcaa aacctgccgg agcgaatttg tcggttgcac cgtggatcac taacaggaag
    36241 cccgcactca ataccagttc aactaccagc gcggaaagca tggaataacc gcctggtgaa
    36301 tgctcgccat aaccgttaga agcaaaaccg ctggctgccg cgtcaaaacc cgttttacca
    36361 ctggcaatta aatacagcag cgccgctgca acaataccgc cgacaacctg ggcaattacg
    36421 tagccaacga cttcttttgc cggaaaacgt ccgccagccc ataaaccaat agtgaccgcc
    36481 gggttaaaat gaccaccaga aatatgacca acagcgaagg ccatcgtcag aacggtcaaa
    36541 ccaaatgcca gcgccacgcc ggcaaaacca atgcctaatt ccgggaagcc tgcggccagt
    36601 acagcactac cacagccacc aaaaacaagc cagaaagtac caaaacattc agctgctaat
    36661 tttctgaaca tatccacctc aattaaa
//
LOCUS       DAJVPP010000032        34956 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVPP010000032 DAJVPP010000000
VERSION     DAJVPP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.032"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..773)
                     /locus_tag="OQM34_001575"
     CDS             complement(<1..773)
                     /locus_tag="OQM34_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1452072.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNP"
     gene            865..951
                     /locus_tag="OQM34_001576"
                     /pseudo
     CDS             865..951
                     /locus_tag="OQM34_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1356..1832)
                     /locus_tag="OQM34_001577"
     CDS             complement(1356..1832)
                     /locus_tag="OQM34_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1452073.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1891..3180)
                     /gene="bioA"
                     /locus_tag="OQM34_001578"
     CDS             complement(1891..3180)
                     /gene="bioA"
                     /locus_tag="OQM34_001578"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1452074.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QMAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3267..4307
                     /gene="bioB"
                     /locus_tag="OQM34_001579"
     CDS             3267..4307
                     /gene="bioB"
                     /locus_tag="OQM34_001579"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1452075.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4304..5458
                     /gene="bioF"
                     /locus_tag="OQM34_001580"
     CDS             4304..5458
                     /gene="bioF"
                     /locus_tag="OQM34_001580"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1452076.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5445..6200
                     /gene="bioC"
                     /locus_tag="OQM34_001581"
     CDS             5445..6200
                     /gene="bioC"
                     /locus_tag="OQM34_001581"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1452077.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6193..6870
                     /gene="bioD"
                     /locus_tag="OQM34_001582"
     CDS             6193..6870
                     /gene="bioD"
                     /locus_tag="OQM34_001582"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1452078.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7449..9470
                     /gene="uvrB"
                     /locus_tag="OQM34_001583"
     CDS             7449..9470
                     /gene="uvrB"
                     /locus_tag="OQM34_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1452079.1"
                     /translation="MSKPFKLNSAFKPSGDQSEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9662..10570)
                     /gene="yvcK"
                     /locus_tag="OQM34_001584"
     CDS             complement(9662..10570)
                     /gene="yvcK"
                     /locus_tag="OQM34_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1452080.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10835..10979
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10967..11956
                     /gene="moaA"
                     /locus_tag="OQM34_001585"
     CDS             10967..11956
                     /gene="moaA"
                     /locus_tag="OQM34_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1452081.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11978..12490
                     /gene="moaB"
                     /locus_tag="OQM34_001586"
     CDS             11978..12490
                     /gene="moaB"
                     /locus_tag="OQM34_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1452082.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12493..12978
                     /gene="moaC"
                     /locus_tag="OQM34_001587"
     CDS             12493..12978
                     /gene="moaC"
                     /locus_tag="OQM34_001587"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1452083.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12971..13216
                     /gene="moaD"
                     /locus_tag="OQM34_001588"
     CDS             12971..13216
                     /gene="moaD"
                     /locus_tag="OQM34_001588"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1452084.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13218..13670
                     /gene="moaE"
                     /locus_tag="OQM34_001589"
     CDS             13218..13670
                     /gene="moaE"
                     /locus_tag="OQM34_001589"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1452085.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13807..14511
                     /locus_tag="OQM34_001590"
     CDS             13807..14511
                     /locus_tag="OQM34_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1452086.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14716..15429
                     /locus_tag="OQM34_001591"
     CDS             14716..15429
                     /locus_tag="OQM34_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1452087.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15465..16421)
                     /locus_tag="OQM34_001592"
     CDS             complement(15465..16421)
                     /locus_tag="OQM34_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCS1452088.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16421..17662)
                     /gene="clsB"
                     /locus_tag="OQM34_001593"
     CDS             complement(16421..17662)
                     /gene="clsB"
                     /locus_tag="OQM34_001593"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1452089.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17659..18420)
                     /locus_tag="OQM34_001594"
     CDS             complement(17659..18420)
                     /locus_tag="OQM34_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1452090.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18553..18963
                     /locus_tag="OQM34_001595"
     CDS             18553..18963
                     /locus_tag="OQM34_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1452091.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18925..20031)
                     /locus_tag="OQM34_001596"
     CDS             complement(18925..20031)
                     /locus_tag="OQM34_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1452092.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20042..21175)
                     /locus_tag="OQM34_001597"
     CDS             complement(20042..21175)
                     /locus_tag="OQM34_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1452093.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21168..22904)
                     /locus_tag="OQM34_001598"
     CDS             complement(21168..22904)
                     /locus_tag="OQM34_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1452094.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22897..23892)
                     /gene="hlyD"
                     /locus_tag="OQM34_001599"
     CDS             complement(22897..23892)
                     /gene="hlyD"
                     /locus_tag="OQM34_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1452095.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23895..24566)
                     /gene="cecR"
                     /locus_tag="OQM34_001600"
     CDS             complement(23895..24566)
                     /gene="cecR"
                     /locus_tag="OQM34_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1452096.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24795..25982
                     /gene="rhlE"
                     /locus_tag="OQM34_001601"
                     /pseudo
     CDS             24795..25982
                     /gene="rhlE"
                     /locus_tag="OQM34_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26189..26671)
                     /gene="ybiA"
                     /locus_tag="OQM34_001602"
     CDS             complement(26189..26671)
                     /gene="ybiA"
                     /locus_tag="OQM34_001602"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1452097.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26791..28941
                     /gene="dinG"
                     /locus_tag="OQM34_001603"
     CDS             26791..28941
                     /gene="dinG"
                     /locus_tag="OQM34_001603"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1452098.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28969..29931
                     /gene="ybiB"
                     /locus_tag="OQM34_001604"
     CDS             28969..29931
                     /gene="ybiB"
                     /locus_tag="OQM34_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1452099.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30072..31157
                     /gene="hcxB"
                     /locus_tag="OQM34_001605"
     CDS             30072..31157
                     /gene="hcxB"
                     /locus_tag="OQM34_001605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1452100.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31386..31646)
                     /locus_tag="OQM34_001606"
     CDS             complement(31386..31646)
                     /locus_tag="OQM34_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1452101.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31910..32176)
                     /gene="ybiI"
                     /locus_tag="OQM34_001607"
     CDS             complement(31910..32176)
                     /gene="ybiI"
                     /locus_tag="OQM34_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1452102.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32250..32927)
                     /gene="ybiX"
                     /locus_tag="OQM34_001608"
     CDS             complement(32250..32927)
                     /gene="ybiX"
                     /locus_tag="OQM34_001608"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1452103.1"
                     /translation="MMYHIPDVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32969..34956)
                     /locus_tag="OQM34_001609"
                     /pseudo
     CDS             complement(32969..34956)
                     /locus_tag="OQM34_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8283 a         8966 c         9443 g         8264 t
ORIGIN      
        1 aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat actgtctggc
       61 aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg aagttcgggc
      121 agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg aaggggcggt
      181 agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg taattctggt
      241 aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg aagatcaggt
      301 agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg aagagaaggc
      361 aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg cggaagagcc
      421 gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg agggagttct
      481 ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg gggcaggtat
      541 gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa agccagacat
      601 attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc cttggcccag
      661 ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa acgggatata
      721 catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt catttattat
      781 tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat tctttaaaaa
      841 aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct ggccgaaaat
      901 gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg ttggtcgccc
      961 taccttttca tcaagccagt agtctgccca ccacagcatc atttctctgc gcttatcgag
     1021 atactgagca tggttgtaaa ttcagctaac ccagtgcgtt gacaaaaaat tagcgcaaga
     1081 ggacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca tctaagtggt
     1141 tgattcatag tgactggata tgttgtgttt tgcagtatta tgcagtctat tttttaagct
     1201 aaatgtatta taatgcattg atattaatga tttttactgt ttctcggtca gcttttttat
     1261 actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat acctttaagt
     1321 gataccagat ggcattgcgc catctggcag agtgattaac taaacatcgc ggtaatcgat
     1381 gcgcttgcca gagagtggaa atgaacgtta aacccgacca tcgcgccgct ggctccttca
     1441 tcgacatcaa tacgctctac atccagcgcg tgaacggtaa aaatgtagcg atgggtttcg
     1501 cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa aatcggtacg cgtctgcaaa
     1561 acgccgtctg gcattgctac cagaccagag ccaaaccctt gcggtaatac gcgggtatca
     1621 gcgggtaaat taacaactac ccagtgccac cagccggagc cggttggcgc atccgggtcg
     1681 tagcaggtga caacaaaact tttcgttccc gcaggaacat catcccacgc cagatgcggt
     1741 gaaatattat cgccatcgta acccatgccg ttaaagacat gacgatgcgg caacttatcg
     1801 ccatcgcgca gatcgttact gatgagtttc atgaaccctc ctttcttgtt tgcagaaagt
     1861 gtagccagaa accctcacgc tgacttcccg ttattggcaa aaaaatgttt catcctgtac
     1921 cgcgcggtta accgctgcgg tcagacgctg caactgttgc gggagaataa tatagggcgg
     1981 catcaggtaa atcagtttgc caaaaggccg gatccatacc ccctgttcga caaagaattt
     2041 ttgcagcgcc gccatattca ccggacgagt ggtttcgacc acaccgattg cccccagtac
     2101 gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc agttgctcgc gcagctgcac
     2161 ttcaatagcc gccatctgct gctgccattc gccggattcg ataatcgcta ggctggcgtt
     2221 tgctgccgcg caggccagcg gattgcccat aaaagttggc ccatgcataa agcagccggc
     2281 ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta agtgtggcgg aaagggtcat
     2341 tgtgccgccg gttaaggctt taccgaggca caaaatgtcc ggcgcgattt ctgcatattc
     2401 acaggcaaac agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg caatcagcaa
     2461 gataccttcg cgatcgcaca ttttgcggat tcgttttaac cattccggat ggtacatgcg
     2521 catcccgcct gcgccctgga caatcggctc aatgatcacc gctgcgattt catgacgatg
     2581 cgccgccatc aggcgggcaa aaccaaccat atcgcgttca tcccattcac catccatgcg
     2641 gctttgcggg gcgggagcaa acaagttttc tggcagatag cctttccaca ggctgtgcat
     2701 tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat aaccattgcg
     2761 gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc cagtactgca acgccatttt
     2821 catcgccact tccaccgcta cggaaccgga gtccgcgaga aaaacgcact ccagcggttg
     2881 cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg tgataccgcc
     2941 aaacatcaca tgcgacatgg catcaatttg cgtcttcatc gccgcattaa gctgcgggtg
     3001 attgtagccg tggatcgccg cccaccagga cgacataccg tcaaccaggc gtctgccgtc
     3061 agacaaaatt agctcgcaac cttcggcgct cgccaccgga taaaccggca gaggggaggt
     3121 catggatgtg tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt ccgttgtcat
     3181 aatcgacttg taaaccaaat tgaaaagatt taggtttaca agcctacacc gaattaacaa
     3241 caaaaaacac gttttggaga agccccatgg ctcaccgccc acgctggaca ttgtcgcaag
     3301 tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag caggtgcatc
     3361 gccagcattt cgaacctcgt caggtgcagg tcagcacgtt gctgtcgatt aagaccggag
     3421 cttgtccgga agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc gggctggaag
     3481 ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg aaagcggcag
     3541 gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc ccacgaacgc gatatgccgt
     3601 acctggaaca aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt atgacgctgg
     3661 gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc cgggctggat tactacaacc
     3721 acaacctgga cacctcgccg gagttttacg gcaatatcat caccacacgc acttatcagg
     3781 aacgcctcga tacgctggaa aaagtgcgcg atgccgggat caaagtctgc tcaggtggca
     3841 ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa ctggcaaacc
     3901 tgccgacgcc gccggaaagc gtaccaatca acatgctggt gaaggtgaaa ggcacgccgc
     3961 ttgccgataa cgatgacgtt gatgcctttg attttattcg caccattgcc gtagcgcgga
     4021 tcatgatgcc gacctcttac gtgcgccttt ctgccggacg cgagcagatg aacgaacaga
     4081 ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt ctacggttgc aaactgctga
     4141 ccacgccgaa tccggaagaa gataaagacc tgcaactgtt ccgcaaactg gggctaaatc
     4201 cgcagcaaac tgccgtgctg gcaggggata acgaacaaca gcaacgtctt ggacaggcgc
     4261 tgatgacccc ggacaccgac gaatattaca acgcggcagc attatgagct ggcaggagaa
     4321 aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc gttatccggt
     4381 ggcgcaagga gccggacgct ggctggtggc ggatgatcgc cagtatctga acttttccag
     4441 taacgattat ttaggtttaa gccatcatcc gcaaattatc cgtgcctgga agcagggggc
     4501 ggagcaattt ggcatcggta gcggcggctc cggtcacgtc agcggttata gcgtggtgca
     4561 tcaggcgctg gaagaagaac tggccgagtg gcttggctat tcgcgggcgc tgctgtttat
     4621 ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg atggcgaaag aggaccgtat
     4681 tgttgccgac cggcttagcc atgcctcatt gctggaggct gccagtttaa gcccgtcgca
     4741 gcttcgccgt tttactcata acgatgtcgc tcatctggcg cgactgcttg cttccccctg
     4801 tccggggcag caactggtgg tgacagaagg cgtgttcagc atggacggcg atagcgcgcc
     4861 actggcggaa atccagcagg taacgcaaca gcacaatggc tggttgatgg tcgatgatgc
     4921 ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc tgctggctgc aaaaggtaaa
     4981 accagaattg ctggtagtga cttttggcaa aggatttggc gtcagtgggg ctgcggtgct
     5041 ttgctcaagt acggtggcgg attatctgct gcaattcgcc cgccacctta tctacagcac
     5101 cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg ctggcggtca ttcgccgtga
     5161 tgagggggat gcacggcgcg aaaaactggt gtcactcatt gcgcgttttc gtgccggagt
     5221 acaggatttg ccgtttacgc tggctgattc atgcagcgcc atccagccat tgatcgtcgg
     5281 tgataacagc cgtgcgttac aactggcaga aaaactgcgc cagcaaggct gctgggtcac
     5341 ggcgattcgc ccgccaaccg tacccgctgg tactgcgcga ctgcgcttaa cgctaaccgc
     5401 tgcgcatgaa atgcaggata tcgaccgtct gctggaggtg ctgcatggca acggttaata
     5461 aacaagccat tgcagcggca tttggtcggg cagccgcaca ctatgagcaa catgcagatc
     5521 tacagcgcca gagtgctgac gccttactgg caatgcttcc acagcgtaaa tacacccacg
     5581 tactggacgc gggttgtgga cctggctgga tgagccgcca ctggcgggaa cgtcacgcgc
     5641 aggtgacggc gttagatctc tcgccgccaa tgcttgcgca agcccgccat aaagatgccg
     5701 cagatcatta tctggcagga gatatcgaat ccctgccgtt agcgactgcg acgttcgatc
     5761 ttgcatggag caatctcgcg gtgcagtggt gcggtaattt atccacggca ctccgcgagc
     5821 tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac cacgctggtg cagggatcgt
     5881 tacccgaact gcatcaggcg cggcaggcgg tggacgagcg tccgcatgct aatcgctttt
     5941 taccgccaga tgaaatcgaa gagtcgctga acggcgtgcg ttatcaacat catattcagc
     6001 tcatcacact atggtttgat gatgcgctca gtgccatgcg ttcgctgaaa ggcatcggtg
     6061 ctacgcatct tcatgaaggg cgcgacccgc gaatattaac gcgttcgcag ttgcagcgat
     6121 tgcaactggc ctggccgcaa caacaggggc gatatcctct gacgtatcat ctttttttgg
     6181 gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa cggataccga agtggggaaa
     6241 accgtcgcca gttgtgcact tttacaagcc gcgaaggcag taggttatcg gacggcaggt
     6301 tataaaccgg tcgcctctgg cagcgaaaag accccggaag gcttacgtaa tagtgacgcg
     6361 ctggcgttac agcacaacag cagcctgcag ctggattacg caacggtaaa tccttacacc
     6421 ttcgcagaac ccacctcgcc gcacatcatc agcgcccaag agggcaggcc gatagaatca
     6481 ttggtaatga gcgccggatt acgcgcgctt gaacaacagg ctgactgggt gttagtggaa
     6541 ggtgctggcg gctggtttac gccgctttct gacactttca cttttgcaga ttgggtaaca
     6601 caggaacaac tgccggtgat actggtagtt ggtgtgaaac tcggctgtat taatcacgcg
     6661 atgttgactg cgcaggcaat acaacacgcc ggactgactc tggcgggttg ggtggcgaac
     6721 gatgttacgc ctccgggaaa acgtcacgct gaatatatga ccacgctcac ccgcatgatt
     6781 cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa atccagaaaa tgcggcaacc
     6841 ggaaagtaca taaaccttgc cttgttgtag ccattctgta tttggttaaa ttgcgagcga
     6901 gatcgcgtct tcgattgact gcaatttaac caattaaatt ctaaaataat cacgaaaaaa
     6961 attttacttc cgcctcatgc ggcgaatgtg ggaattgccc aggcggcggg ggataggggc
     7021 tggagacagt tatccactat tcctgtggat aaccatgtgt attagagtta gaaaacacga
     7081 ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt taaagacggc tcaaagaaat
     7141 atcttttatt ttttaactgg ttagataaat gcaatggcag tcactgaaca ggcatctctt
     7201 gccataaaac tgtcatcact catcttgaca aatgttaaaa aagccgttgc tttggggata
     7261 acccggtaag gctggagttt tatctcgcca cagagtaaat tttgctcatg attgacagcg
     7321 gagtttacgc tgtatcagaa atattatggt gatgaactgt ttttttatcc agtataattt
     7381 gttggcataa ttaagtacga cgagtaaaat tacatacctg cccgcccaac tccttcaggt
     7441 agcgactcat gagtaaaccg ttcaaactga attccgcttt taaaccttct ggcgatcagt
     7501 cagaggcgat tcgacgtctc gaagaggggc tggaagatgg cctggcgcac cagacgttac
     7561 ttggcgtgac tggctccggg aaaaccttca ccattgccaa tgtcattgct gaccttcagc
     7621 gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc ccagctgtat ggcgaaatga
     7681 aagagttctt cccggaaaac gcggtggagt atttcgtctc ctactacgac tactatcagc
     7741 cggaagccta tgtaccgagt tccgacacct tcattgagaa agatgcctcg gttaacgaac
     7801 atattgagca gatgcgtttg tccgccacca aagcgatgct ggagcggcgt gatgtgattg
     7861 tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc tgatttatat ctcaagatga
     7921 tgctccatct cacggtcggt atgattatcg atcagcgcgc gattctgcgc cgactggcgg
     7981 agctgcaata cgctcgtaat gatcaagcat tccagcgtgg tactttccgc gttcgtggcg
     8041 aggtgataga tatcttcccg gcagaatcgg atgacattgc acttcgcgtg gaactgtttg
     8101 acgaggaagt ggaacgattg tcgttatttg acccgctgac cgggcagatt gtttccacta
     8161 ttccacgttt taccatctac ccgaaaacgc actacgtcac accgcgcgag cgcatcgtac
     8221 aggcgatgga ggagatcaaa gaagaactgg ccgccagacg caaagtgctg ttggaaaaca
     8281 acaaactgct ggaagagcag cggctgaccc agcgtaccca gtttgatctg gagatgatgg
     8341 acgagctggg ctactgttcg gggattgaaa actactcgcg cttcctctcc ggtcgtggac
     8401 cgggtgagcc accgccgacg ctgtttgatt acctgcctgc cgatgggctg ctggtcgtcg
     8461 atgaatctca cgtcaccatt ccacaaattg gcggcatgta tcgcggtgac cgggcgcgta
     8521 aagagacact ggtggagtac ggcttccgcc tgccatcagc gctggataac cgtccgctta
     8581 agtttgaaga gttcgaagca ttagcgccgc aaaccatcta tgtttcggcg acgccgggta
     8641 attacgagct ggaaaaatcc ggcggcgatg tggtggatca ggtggtgcgt ccaaccggat
     8701 tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca ggttgatgat cttctttcgg
     8761 agattcgtca gcgagcggca attaacgaac gcgtgctggt caccacactg accaagcgaa
     8821 tggcggaaga tcttaccgaa tatctcgaag aacacggtga gcgcgtgcgt tatcttcact
     8881 cagatatcga caccgtcgag cgtatggaga ttatccgcga cttgcgtctg ggtgagttcg
     8941 acgtattggt agggatcaac ttactgcgtg aaggtctgga tatgccggaa gtgtcgctgg
     9001 tggcgatcct cgacgctgac aaagaaggct tcctgcgttc cgaacgttcg ttgatccaga
     9061 ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat tctctacggc gataggagca
     9121 ctccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg ccgtgagaaa cagcagaagt
     9181 acaacgagga acacggcatt acgccgcaag gcttgaacaa gaaagtggtc gatatcctgg
     9241 cgctggggca gaacattgcc aaaaccaaag cgaagggcag aggaaaatcg cgcccgattg
     9301 ttgagccgga taatgtgccg atggatatgt tgccgaaagc gttgcagcag aaaatccatg
     9361 aactggaagg gctgatgatg caacacgcgc agaatctgga gttcgaagaa gcggcacaaa
     9421 ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc cgcatcgtaa caggatagcg
     9481 aagaagactg atgacaaacg gaaaacagtc tgatgcgcta cgcttatcag gcctacattt
     9541 tctccgcaat atattgaatt tgcgcggttt gtaggccgga taaggcgttc acgccgcatc
     9601 cggcataaac aacgcgcact ttgtcttcaa taggaagccg gaattttcct tcggatttcc
     9661 gttaacctaa agcctgtaac gccttttcca gcgcgttatg taacaactgg cggtcatgac
     9721 gatacggaat atcgctggcc tccagtacct cctggatcac aatccgctct ttcaccgccg
     9781 agacatccac ttttggcccg acgatgaccg catcaatgac ctttttacca acatactgct
     9841 ccataattgc cagcttgctt tccagcttca aattagccgc aggtaaactc aactcacgcc
     9901 ccagattgcc gatataaacc atcggcgctg gcgtgcggcg taatgcctgg gcgatttcct
     9961 tcagcagcag aattggcatc aggctggtat aaaaactgcc aggcccaata atgatgagat
    10021 ccgcttcatt gatagcgtga accgcctcac gcgttgcggg tacattaggc gttaacaata
    10081 actcttgaat cggcgtagtt aactggtcga tattgacctc gccgtaaact tcatgcccct
    10141 gatcgtcaat cgccatcaga tcaacaggat gctctgacat tggaatcaaa tgcgtatcca
    10201 ctttcagcag attacgaatt aaattgatgg cttccagagg ccgcacgcta aggtgatcca
    10261 gcgcctttaa catcaagttt ccgagattat gaccggaaag ttcgccattg ccgccaaaac
    10321 ggtattcaaa catcgcggag gcgacgttcg gttccgttat cagctggttg aggcagttgc
    10381 gcatatcgcc ccaagcaatg ccgccttctg aacggcgaat acgccccgtc gagccaccat
    10441 tatccgtggt ggtgacgata cccgttaaac gagaacccaa agacgaaagt gatgagagaa
    10501 cgcgtcccag tccatgccct ccgccgagag caacgacacg atcaagatca gccagcgtac
    10561 gattgcgcat atagtttcct ggagtcagat tatccgcgct acagtagcgc aaattgcggg
    10621 gaaacagcaa ttaacctgcc aatattgagg atacaaacta ttttcttcta tctctaaagg
    10681 acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt aaaaccgcca ttttccctta
    10741 ttctgtagcg aaatagcacg atcatgacgc tatatacatg attacatagc gaaagtgtgg
    10801 atggtaaaaa tctcatttac acgctagtat cggcttaacc actaaacact ctagcctctg
    10861 cacctgggtc aactgatacg gtgctttggc cgtgacaatg ctcgtaaaga ttgccaccag
    10921 ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag gtgtacatgg cttcacaact
    10981 gactgatgca tttgcgcgta agttttacta cttgcgcctg tcgattaccg atgtgtgtaa
    11041 ctttcgttgc acctactgcc tgccggatgg ctacaaaccg agcggcgtca ccaataaagg
    11101 ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc ttcgccagtc tgggcaccga
    11161 aaaagtgcgc ctgacaggag gagagccgtc tttacgccgc gactttaccg atatcatcgc
    11221 cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc accaccaatg gttaccgtct
    11281 ggaacgcgat gtggcgaact ggcgcgatgc gggacttact ggcattaacg tcagtgtcga
    11341 cagtttggac gcccgccagt ttcacgctat taccgggcag gataaattca accaggtcat
    11401 ggcagggatt gatgctgcat ttgaggccgg ttttgagaag gtcaaagtca ataccgtgct
    11461 gatgcgtgat gttaatcatc accagctcga cacctttctg aactggatcc agcatcgccc
    11521 tatccagctg cgtttcatcg aactgatgga aacgggcgag ggcagcgagc tcttccgtaa
    11581 gcatcacatc tctggtcagg ttctgcgtga cgagctactg cgtcgcggct ggatccacca
    11641 attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc catccagatt acgccggaga
    11701 gattggcctt atcatgccgt atgaaaaaga cttctgcgcc acttgcaacc gcctgcgcgt
    11761 ttcctccatt ggtaaactcc atctctgcct gtttggtgaa ggcggcgtta acctgcgcga
    11821 tctgctggaa ggcgataccc agcaacaggc gctggaagcg cgtatttcag cggcgctgcg
    11881 ggagaagaaa cagacccatt tcctgcatca aaacaacacc ggtattacgc aaaacttatc
    11941 gtacattggc ggctaaaacg tcaaaaggag agatcagatg agtcaggtaa gcactgaatt
    12001 tatcccgacc cgtattgcta ttcttacggt ttctaatcgt cgcggtgaag aagacgatac
    12061 ctccggtcac tatctgcgcg attcggcgca agaagcgggc catcacgttg tcgatgaagc
    12121 cattgtgaaa gaaaaccgct acgctattcg cgctcaggta tctgcgtgga tcgccagcga
    12181 cgatgtacaa gtggtattga ttacgggtgg tactggcctg acggaaggtg atcaggctcc
    12241 cgaagcgctg ctgccgttgt tcgaccgtga ggttgaaggt tttggtgaag tgttccgtat
    12301 gttgtcgttt gaagagattg gcacttccac gttgcaatct cgtgcggtag cgggcgtcgc
    12361 taacaaaacg ctgattttcg ccatgccggg ttcgaccaaa gcgtgccgta ccgcatggga
    12421 aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg tgtaatttcc atccacattt
    12481 gaagaaataa gtatgtcgca actgacccat atcaacgccg ctggcgaagc gcacatggtg
    12541 gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg cggaagcctt tgtcaccatg
    12601 cgcagcgaga cgctggcgat gattattgat ggtcgccacc acaaaggcga cgtatttgcc
    12661 actgcgcgta ttgccggtat tcaggcggca aaacgcactt gggatctgat cccgctgtgt
    12721 cacccgctga tgctcagcaa agttgaagtc aatttacagg ccgagccgga gcacaatcgg
    12781 gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg gtgtcgaaat ggaagcgtta
    12841 accgcggcct ccgtggcggc gctgaccatt tatgacatgt gcaaagcggt gcaaaaagat
    12901 atggtgattg gtccggtacg tttgctggcg aagagcggcg gcaagtcggg tgactttaag
    12961 gtggaagcgg atgattaaag ttcttttttt cgcccaggtg cgcgagttgg tgggaacaga
    13021 tgcaaccgaa gtggctgcgg attacccaac cgttgaagcg ttacgccagc acctggctgc
    13081 gcagagcgat cgctgggcgc tggcgctgga agatggcaaa ttgctggctg ccgtcaacca
    13141 gacgctggtg agttttgacc atccgctgac tgacggcgac gaagtagctt tcttcccgcc
    13201 ggtaaccgga ggttaagatg gcagaaacca aaattgttgt cggcccgcag ccgttcagcg
    13261 taggagaaga gtacccgtgg ctggcggagc gtgacgaaga cggtgcggta gtcaccttca
    13321 ccggaaaggt gcgcaaccat aacctgggcg acagcgtcaa agcattaacc ctcgaacact
    13381 atccggggat gactgaaaaa gcactggcag aaattgttga tgaagcgcgt aaccgctggc
    13441 cgctggggcg cgtcacggtg attcaccgca tcggggaatt atggccgggc gatgaaatcg
    13501 tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt tgaagccggg cagtttatta
    13561 tggattatct caaaacccgc gcaccgttct ggaagcgcga agccacgccg gaaggcgacc
    13621 gctgggttga agcccgggag agcgatctgc aggcggcaaa acgctggtag ttttttgtta
    13681 gccggataag gcaccgctgc gtccggcaat ttcaccgctc agtacatgcg tttacgctgc
    13741 gtcgatgcac agcctcatca ttttgcagta tccttaagat attccttata tcttcaggag
    13801 atcgtcatgg acagattccc acgttctgat tcaatcgtac aaccccgggc tggcttgcaa
    13861 acttatatgg cgcaagtcta tggctggatg accgttggct tgttgctgac cgcatttgtt
    13921 gcctggtatg cggctaattc cgcggccgtg atggagctgt tgttcactaa ccgtgtcttt
    13981 ttaatcggtc tgatcatcgc gcaattagca ttggttattg tgttatcagc gatgattcaa
    14041 aagctgagcg caggtgtaac gacgatgctc tttatgcttt attcggcgct gacgggtctt
    14101 acgctttcca gtatattcat tgtctatacc gctgcttcta tcgccagtac tttcgtcgtt
    14161 actgccggga tgttcggcgc aatgagcctg tacggttaca ccacgaagcg cgatttaagt
    14221 ggctttggca atatgctgtt tatggcgtta atcggcattg tgctggcatc gctggtcaac
    14281 ttctggctga aaagcgaagc attgatgtgg gcagttacct acatcggcgt gattgtcttt
    14341 gtcggattga cggcgtatga cactcagaaa ttgaaaaata tgggtgagca gattgatacc
    14401 cgcgacacgt cgaacctgcg caaatattcc attcttggtg cgttaacctt gtatctggac
    14461 ttcatcaacc tgttcctgat gttgttacgg atcttcggca accgccgtta attctttttc
    14521 accatcacta tcccggccat gtgtcgggat ttttttctta ctaaaaacga cacttaacaa
    14581 gtaccaggta taaaatatgc cttatgagcg agcggaatcg ttttccgtat ccataaatac
    14641 aaaagtattt tgtatgcgtg gttttaaatt tattgatggt gaatattaat gttttttcta
    14701 aggacaacta aaagaatgga atcatactcg caaaacagca ataaattaga ttttcagcac
    14761 gaggccagga tattaaacgg tatatggctc attaccgctt taggtttggt ggcaaccgca
    14821 ggactagcct ggggagctaa gtatctcgaa attacggcaa ccaaatatga ttcaccacca
    14881 atgtatgtcg ccatagggtt attattactt tgtatgtatg gcttaagtaa ggatatcaac
    14941 aagataaatg ccgccatcgc gggcgtaata tatctgtttt tactctcttt ggtggcgatt
    15001 gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg tgttcagcac tgcgggcgcg
    15061 atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg ttctcaccgt
    15121 tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa tgcggcatta
    15181 atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgtgttatg gccaggcatt
    15241 atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag tgaagagttg
    15301 tggagtccgg ttcgttgcgc ttttacaggt gcattaacac tctattacta ttttatcggc
    15361 ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca tacgcgtttt
    15421 tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat cgccgcttca
    15481 tttttcgccc gtagcttctt cgcttggctt tccagtagca ggtaacagat cagcgccagc
    15541 agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat aatgctccct
    15601 ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac accgatcccc
    15661 gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc cagtacaaag
    15721 aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac catccagtta
    15781 acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac cagcttttgc
    15841 cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata aaccgcgata
    15901 atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata ccagtaatcc
    15961 ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata gcccagccag
    16021 ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc tgcccggtaa ccctagtcgg
    16081 gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt caggttgaag
    16141 gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg accgcagtaa
    16201 aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac cagcccgacc
    16261 gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc ttcccagtcc
    16321 acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag ccaggtgagg
    16381 atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcaggggttt acccccgtgt
    16441 tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga tcaacctgcg
    16501 tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg cgtaaaaagt
    16561 ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc atggtttcat
    16621 ccacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc gtctggttaa
    16681 aatgacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg agcggatcga
    16741 gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg ccgtggagcg
    16801 ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac cagatagtta tacagcaagc
    16861 gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt ttgatccgca
    16921 ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg aagaagtagg
    16981 cgttggcgat aatcacttcc cgctgcgcct gagtgagcat tttcagataa tggcgttcaa
    17041 tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct tctcccggct
    17101 ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg ctctgtccag
    17161 gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca aggcgtaccg
    17221 cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag taattcagcc
    17281 cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc cggcgaaaca
    17341 cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac actacgccag
    17401 ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag ccatccagca
    17461 agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc agctgtttgc
    17521 cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt gcctcgccaa
    17581 tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg atcttattgc
    17641 cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat gatcagaaag
    17701 gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat ttttgacgta
    17761 gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg ggcgtccgtg
    17821 ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat tagctttttg
    17881 ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta gctcattcac
    17941 ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc ccagatgtac
    18001 gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt agagcacgcc
    18061 gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa tgggataacg
    18121 cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac cgtaggcaaa
    18181 atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc agttttccac
    18241 atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa tatcggcgct
    18301 gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa acgcggtaaa
    18361 gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt gatcgggcat
    18421 aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc acaaaaaggt
    18481 gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga taaattcttc
    18541 aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct aagttgctgg
    18601 cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat gagtaagact
    18661 ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt gatgatgctg
    18721 gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct ggaagagctg
    18781 accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc ccgcgtactg
    18841 gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc ggcggtagtg
    18901 tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg cgtacgccgc
    18961 tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca caatatccag
    19021 actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa tagggttaat
    19081 ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt aaccggaaag
    19141 gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt gtgtcgagca
    19201 gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca ccatcgtaaa
    19261 gtagaacagc gctagcgatc cggcgaaggg gatttgatac gcccagatac caatcgccag
    19321 cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc cgatgaagat
    19381 ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt cacgttcgcg
    19441 ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg cgatcagcga
    19501 cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta ccaccagctc
    19561 gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt tgacgatctg
    19621 ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga ggatcaactg
    19681 caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga aacgcaccag
    19741 tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgtgggcttt tcagcagcag
    19801 cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg aatgctcgcc
    19861 gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg cggcgaacgg
    19921 gaacaggatc acctgaatta gcacgggtaa aatcagaatc gcgcgggttt gtggttcgcg
    19981 cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca tgctcttctc
    20041 cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc accgccgaag
    20101 cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg aggaacaggc
    20161 tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg cggatcaccg
    20221 cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc agaaaagcgg
    20281 cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa atcagcagcc
    20341 ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc agcagcgaac
    20401 cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcaac atcgccagca
    20461 tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg gtaatctccg
    20521 tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc agcgaggtga
    20581 gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc cgggataata aagtgctggc
    20641 ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt tcaaaagtct
    20701 gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg acatacccct
    20761 gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc ggtgcggtgg
    20821 cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga ccgcgaattt
    20881 tccccgactg cattttggcg atcagttcct gacggttatc gctgatggtg gcgtcgatgt
    20941 agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc tgttccagca
    21001 aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa ataaacagca
    21061 gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg atctgccgcg
    21121 tctctttgac gcacagcgcc cgcacgcgat gccaggaaag gatcgggtta ctcattgcta
    21181 tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt gggatcgggt
    21241 tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg taccgctggc gattaatttc
    21301 ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa gtgggtggtg
    21361 accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa ttcacggcgg
    21421 gtgagggggt caacgccgga agtcggttcg tcgagaaaga gaatatccgg ttcatgcatc
    21481 agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc ggtggcgtgt
    21541 gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg agattttttc gttctgcgcc
    21601 cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac cgtcaggttg
    21661 ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt accggaactc
    21721 tctttcagat ccatccccag caccagcgcc tggccggaag tcggcaccag caaaccgcac
    21781 atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc aaaaatctcc
    21841 ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa tttcttggtc
    21901 agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg caatattgca
    21961 cccagcggcg attctgaggt tccggcaccg cccagcaaat caataaacgc atcttcaaaa
    22021 cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg aatatcgtct
    22081 ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat gccatcgctg
    22141 acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg tggactggtc
    22201 atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc ctgatacagc
    22261 aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc gagatacgag
    22321 gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg ccacagttcg
    22381 cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac tttcggttcg
    22441 cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag cttgcccgcc
    22501 aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa agtttgctta
    22561 cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc catcaccgtg
    22621 agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg cagcgcgccg
    22681 tcgtttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg tttcagtaat
    22741 cccgccaaca tccgcatcag tgtggtttta cctgcaccgt ccggccccac caaccccgtc
    22801 acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc catgcccgga
    22861 aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg cctcgtcacc
    22921 gaattgtacc gtcactggca ttccctggcg taacgcatca tcggcgtcgg tcaccacaat
    22981 acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt tcggggtaaa
    23041 ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg ggcgaccatc
    23101 ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt catcaacata
    23161 agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac cttcattgag
    23221 aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg ctatcaacgt
    23281 tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt cgaggctggc
    23341 tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca atttatcctg
    23401 tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt ccaggtcatt
    23461 tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt tctgcgcata
    23521 gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt cttcatcgcg
    23581 atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg ctttcgcctg
    23641 catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc ccgctttgat
    23701 agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga aactaagatt
    23761 caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc ggctttgata
    23821 ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc cgatcacgac
    23881 aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag gtcgatatga
    23941 caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt ccagccggta
    24001 cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat caacgcatgg
    24061 gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg tccaggcggc aatcaggcgt
    24121 gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta ggctgccgtg
    24181 ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc ctgggtgagc
    24241 agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggcagc ccgatcaggc
    24301 tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg ctcgccaata
    24361 aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc gaagtagtag
    24421 gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt ggcgttcatt
    24481 ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc ctgttcaccc
    24541 ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat gattgattaa
    24601 gattatgact ccatatggga gttgtccagt atggctaaga attttagcta cgccagtcac
    24661 agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc atgacattgt
    24721 ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa cggtcggggc
    24781 ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc ctgcgcgccg
    24841 ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc cctgcggtgc
    24901 tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca gcgggcttta
    24961 cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg cgtcgtccgg
    25021 tacgtgcgct cattcttacc ccgacccgtg aactggcggc acagattggc gaaaacgtcc
    25081 gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggcggt gtcagcatta
    25141 acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg ccgggacgtt
    25201 tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc ctcgtcctcg
    25261 atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc gtgttaacaa
    25321 aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac gatattaaag
    25381 ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc cgcaataccg
    25441 cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa cgcgaattgc
    25501 tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc cgtaccaaac
    25561 acggcgctaa ccatctggct gagcagctca ataaagatgg catccgtagt gtggcgatcc
    25621 acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa tcgggtgaca
    25681 ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa gagttgccgc
    25741 acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt atcgggcgta
    25801 ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat gaacacaaac
    25861 tgctgcgtga tatcgaaaaa ctgctggaaa aagagatccc gcgcattgcg attccgggct
    25921 atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag caacgtggcg
    25981 gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc aggcctacgt
    26041 gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg tttacgccgc
    26101 atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa aaatttatct
    26161 gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt tcgcgcaact
    26221 ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct ccccagtaag
    26281 catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag agcacacgca
    26341 gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt tctttaaccg
    26401 actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg gcgaccatgg
    26461 gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt tgtgcctgaa
    26521 aatagtgttc ggaggtgggc caggtattcc cgtcaacctt gatgggcctg gcggcaaagt
    26581 tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg tcttgcatga
    26641 cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt ggttattgcg
    26701 agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg ccacaatatt
    26761 ggctgtttat acagtatttc aggttttctc atggcattaa ccgccgcgct taaagcgcaa
    26821 attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc ccgtgcgccg
    26881 cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg gcggcatctg
    26941 gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc cggcatcgcc
    27001 attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc gttgcaggat
    27061 cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct taaattcact
    27121 gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc tgacggcgct cgccagcact
    27181 gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc aaataatcag
    27241 gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa atgggatggt
    27301 ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt gagtaccgac
    27361 aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt ttttgttact
    27421 cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt gatggcggcg
    27481 atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct ggacgaaggt
    27541 catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat caccgcgccc
    27601 tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat ggagcagttt
    27661 cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca ttgtgaagag
    27721 ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc tgccgggcag
    27781 gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga gatctgccag
    27841 cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct taacgattta
    27901 agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt acagatgaac
    27961 cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc tctggcgcaa
    28021 tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg gcagctacac
    28081 ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct gctgtggcgc
    28141 agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag tttttcgcgt
    28201 ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc gctggattcc
    28261 ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt tgagccttcc
    28321 atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga acaggtggag
    28381 agcaaaaaat atctcggtat gttggtactg tttgccagcg gacgggcgat gcagcgcttt
    28441 ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca gccgcgttat
    28501 cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt gctggtgggc
    28561 ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca ggtgcatatc
    28621 cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg ggaatggctg
    28681 aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc gtttaacctg
    28741 attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt ggttatctac
    28801 gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt accggtattt
    28861 ccgatagagc aaccggaagt ccctgaaggt atagttaaaa agaaagaaaa aacgaaatcc
    28921 ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat ggactatcgc
    28981 aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga ccgggatact
    29041 gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga gttgggcggc
    29101 gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg cttttacgaa
    29161 gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat gccgattgtc
    29221 atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt ggcgattctg
    29281 ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc aactcgcgtg
    29341 ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg cgggcaggcg
    29401 caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt ttgcccgccg
    29461 ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc acataccctg
    29521 gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt gtctttccag cgtttcgcat
    29581 ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg ggcgttacta
    29641 atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca gatcaatctc
    29701 attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg tagcgagtta
    29761 ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg cctggctggc
    29821 agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt ggctacgggt
    29881 gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcatttta atttttatct
    29941 cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat tcctgcatct
    30001 ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca taacataaac
    30061 aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac agttttattc
    30121 aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt gccgatcatt
    30181 taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc ccaagctatg
    30241 tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc gttaaagagg
    30301 cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg gcacacgaag
    30361 cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg gcgctacata
    30421 actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg gcggggtttg
    30481 tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc cacggtcgcg
    30541 acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat aatttcccgc
    30601 tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc gcctggcata
    30661 aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg acgaccaatc
    30721 cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa cataaaggct
    30781 acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt aaaacgacgc
    30841 atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc actatcatca
    30901 tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc tttgccgagt
    30961 gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc gagtgggaag
    31021 tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga agctggcagg
    31081 ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag gctttctgtc
    31141 agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca agggtaacat
    31201 aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga aatacttgtg
    31261 ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg cctgataaga
    31321 cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct tatccgtcct
    31381 acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt ggcggaagtg
    31441 atgctgtatc cactggcacc ggctgccgcc gctttctcag ccagtttcgc ttccagggca
    31501 tcgagggtgg atgcgccatc ggcagaaacc acgccgattt tattcatgtt ctgtgcctgg
    31561 gatgccgtta ccggttccgc cgcgaacacg ccaaatgaca gagttgaaag agccatagca
    31621 gcaacaacag tattgatagt tttcataatt aatctctcgc aggtgatttt tgtataagtg
    31681 acgttgtttc gtcgatgtga tgagtatcac gttttttacg agagataaaa tcgaagagaa
    31741 ttgaccgcct tgttcaaata aattgattga tatctaacat actgataaac catttatttg
    31801 ttgtacttat ggacttgttt tatgccgcgt ggcagtcaca gcagcgttaa aggcacggaa
    31861 tgccacgcaa aatacaaatt atactcgcta tgcatctcgc cagttgtagt taacgtaact
    31921 gtctgtcttt cgaacctctg cgattatatc ctgtatacgc tggtttttgt aaatctttct
    31981 cctgctgaca atgaatacat aagcgcacgc caggaatggc ttcccgacgg gcttgcggga
    32041 tgggggcacc gcactcttca cattcataca ggctttcgcc acgcggaatt tcaccccgag
    32101 cgcgggcaat cgcatcttca attgtactgt tgatctgttc gttgacggcg tcatcgttag
    32161 cccaagcgga tgccatatcg tcctccccat atcaatgctt gtacagttaa gtgtagctaa
    32221 tatagggacg aacctgggca gttcaagcgt cagatctccg accattcccg cagcagatta
    32281 tgataaagat tgagcagcga caggatctct tcattttcac catagcggct tttcagcgac
    32341 tgaatatttt tgtccagttc aaacagcatg gcgcgctttt tatcatcgcg gatcatcgac
    32401 tggatccaca taaatgatgc cactcgtacg ccgcgggtta cgggtgtcac gcaatgcagg
    32461 ctgctggagg gatacaacac cagatcacct gccgggagtt ttacccgatg ttgtccaaag
    32521 gtgtcattaa cgaccagttc gccgccgtcg tagctttgtg gatcgcttaa aaacagcgtg
    32581 gcagaaaggt cagtgcgcat ccagccgttt tgtggatggc tgcgtactgc gccatccaca
    32641 tggaaaccat aggtttcatt gttctgatag cgattaaaca gcggcgtgga aagggtacgc
    32701 ggcaaggcag cggcaaagaa caatgcatgt tggttaaccg cgttcagcac ctcattttgc
    32761 agtgcggcgt ataacgcgct gcgggtgtct acttgctgat tgttcttaac ttgtgcacct
    32821 tgtgctccgg tggtgacgcg tccatccacc cattcggctt gttccagttg ttcgcgaaaa
    32881 tgggcgacgt cctgtggcga taacacgtcg ggaatgtggt acatcataca agttctccaa
    32941 aaagtggggc ctgcgcccca catctgaatc agaaatgcat attggctgtg agcaagaagg
    33001 ttcttggctc gcccgggtga taacggtagc cgctcttgtt gattgaggcg acgtaatcgg
    33061 tatcaaacag gttgtaaacg tttagctgga agtcgagatt gcgattaacc cgatacccca
    33121 gtttggcatc ggcgacccag taaccttctg taaatgctgg cgttcccacc gcgccgtctg
    33181 aacctttatg tatactgccg atatagcgtg cgcccgcgcc aacagagata tcatcggttg
    33241 cctgatattg gctccataag gtgaaggcgt gctccggggt atacggcagc gatgaggaac
    33301 catcctgggc aacatctttg ccgtttttga tggttgcttt ttgctgggta tagccgccaa
    33361 tcacctgcca cgcgggagtg atattccctg ccacggatat ctcatagcct tcgacgcgtt
    33421 tcttaccgta ttgcgagtaa gtcccgtcat cattttgctc aacttcattt tcgatatcag
    33481 tacggaacag cgcggcggtg agcaacaggc gtttatccag aacctgccat ttggtgccaa
    33541 tctcgctggt gttggctttt tgcggtttaa aatcggtgtg gttggcactg ttaccactgc
    33601 cagactgcgc aagggcgaag ttgttgccgc ccggaggctg ctgggaaacg gcatagttaa
    33661 tatagacatt gccgttttcc gtcaggtggt acagcgcccc ggctttccag ttcaccagat
    33721 tgcccgactt ggcggtgtcg acggtggtga ctggagaacc ttttgccaca ccagctgggc
    33781 aggtgatggc accgcgtccg ctgccgccgc aggcggtggc actgtcatat tcagtatgat
    33841 aattatccag acggatcccg ccgttcagct caaaatcacg ggtgatttgc agcgtatcga
    33901 aggcgtaaat tgcgaaggta tccgtctgac cattggcgtt tgcgccgttg cgcgtcaggc
    33961 cgccgggatg aattctgctg tcaggatgat aaatatttac cgccggtaac gtcaccggat
    34021 taacgccata gttagtctgc gtttcacggg taaattccac gccggtactg acatcatgac
    34081 cgatagaacc ggtatagaac gtcgaggtca ggttggtctg gttggtgaga attttattac
    34141 tcacatcttt ggtattcgcc gtgcgtgact aggtccagct attcacatcg ctggtgggct
    34201 gagtaatatt cgacgccccg cccataatcg ccgtcatcag gtaatcctgc tttacgcgcg
    34261 accaacgggt agtattgcga atggtggtgt tatcgttgat gtcgtgctca aaacgcatgg
    34321 tggcggtgtc ggtggtcgaa tcgtcgtaat cggaatccgt gccgtaaaag ttatgagtat
    34381 caacttttcc ggaatgattc agggccgccg ctcccgcaga tggggcagaa tagcccggca
    34441 aaccgatggt cggaatgccg ccgtctggcg tgttgtgctg agtgacatgc agataattaa
    34501 gatacaaacg atttgctgta ccaaggccaa aagcgacaga aggggcgacg ccgtaacgct
    34561 catttttgac tttgtcgcgt ccggcatcgt gggttttttc gcccatcaca ttcaggcgca
    34621 ccgcggtagt atcaccaatg acctgattga cgtccagcgt gccgcggcgg aaccaggcgc
    34681 tgccaatact ggcggaggcg tcaatgccgg aatcattgcg cggctgcttg ctgatcatat
    34741 tgatcgagcc tgtcggtgcg ctgcgcccgt agtcggtgcc ggacggccct ttaatcactt
    34801 cgacctgctc ggtattgaag gtgtcgcgcg agacgctgcc gatatcgcga ataccatcaa
    34861 tataaatact gttagaggta tcggcaccac gcatataaat ggcgtcgcca gtggtggagt
    34921 taccgttctc acccgcaaaa aacgcaccca cgccgg
//
LOCUS       DAJVPP010000033        34775 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVPP010000033 DAJVPP010000000
VERSION     DAJVPP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.033"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(19..94)
                     /locus_tag="OQM34_001610"
     tRNA            complement(19..94)
                     /locus_tag="OQM34_001610"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:His,seq:gtg)
     gene            complement(153..229)
                     /locus_tag="OQM34_001611"
     tRNA            complement(153..229)
                     /locus_tag="OQM34_001611"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Arg,seq:ccg)
     gene            complement(332..1717)
                     /locus_tag="OQM34_001612"
     CDS             complement(332..1717)
                     /locus_tag="OQM34_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1452104.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="OQM34_001613"
     CDS             complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="OQM34_001613"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1452105.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="OQM34_001614"
     CDS             complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="OQM34_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1452106.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="OQM34_001615"
                     /gene_synonym="wecF"
     CDS             complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="OQM34_001615"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1452107.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="OQM34_001616"
     CDS             complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="OQM34_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1452108.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="OQM34_001617"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="OQM34_001617"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1452109.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="OQM34_001618"
                     /gene_synonym="wecD"
     CDS             complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="OQM34_001618"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1452110.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="OQM34_001619"
     CDS             complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="OQM34_001619"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1452111.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="OQM34_001620"
     CDS             complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="OQM34_001620"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1452112.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="OQM34_001621"
     CDS             complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="OQM34_001621"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1452113.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="OQM34_001622"
     CDS             complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="OQM34_001622"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1452114.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="OQM34_001623"
     CDS             complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="OQM34_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1452115.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="OQM34_001624"
     CDS             complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="OQM34_001624"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1452116.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14928..16187)
                     /gene="rho"
                     /locus_tag="OQM34_001625"
     CDS             complement(14928..16187)
                     /gene="rho"
                     /locus_tag="OQM34_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1452117.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="OQM34_001626"
     CDS             complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="OQM34_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1452118.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="OQM34_001627"
     CDS             complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="OQM34_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1452119.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16974..18239
                     /gene="rhlB"
                     /locus_tag="OQM34_001628"
     CDS             16974..18239
                     /gene="rhlB"
                     /locus_tag="OQM34_001628"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1452120.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18373..19857
                     /gene="gppA"
                     /locus_tag="OQM34_001629"
     CDS             18373..19857
                     /gene="gppA"
                     /locus_tag="OQM34_001629"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1452121.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19904..21925)
                     /gene="rep"
                     /locus_tag="OQM34_001630"
     CDS             complement(19904..21925)
                     /gene="rep"
                     /locus_tag="OQM34_001630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1452122.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22012..22293
                     /gene="ppiC"
                     /locus_tag="OQM34_001631"
     CDS             22012..22293
                     /gene="ppiC"
                     /locus_tag="OQM34_001631"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1452123.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22339..23814)
                     /gene="ilvC"
                     /locus_tag="OQM34_001632"
     CDS             complement(22339..23814)
                     /gene="ilvC"
                     /locus_tag="OQM34_001632"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1452124.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23964..24854
                     /gene="ilvY"
                     /locus_tag="OQM34_001633"
     CDS             23964..24854
                     /gene="ilvY"
                     /locus_tag="OQM34_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1452125.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24851..26395)
                     /gene="ilvA"
                     /locus_tag="OQM34_001634"
     CDS             complement(24851..26395)
                     /gene="ilvA"
                     /locus_tag="OQM34_001634"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1452126.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26398..28248)
                     /gene="ilvD"
                     /locus_tag="OQM34_001635"
     CDS             complement(26398..28248)
                     /gene="ilvD"
                     /locus_tag="OQM34_001635"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1452127.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28313..29242)
                     /gene="ilvE"
                     /locus_tag="OQM34_001636"
     CDS             complement(28313..29242)
                     /gene="ilvE"
                     /locus_tag="OQM34_001636"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1452128.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29262..29525)
                     /gene="ilvM"
                     /locus_tag="OQM34_001637"
     CDS             complement(29262..29525)
                     /gene="ilvM"
                     /locus_tag="OQM34_001637"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1452129.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29522..31168)
                     /locus_tag="OQM34_001638"
     CDS             complement(29522..31168)
                     /locus_tag="OQM34_001638"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1452130.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31171..31221)
                     /locus_tag="OQM34_001639"
     CDS             complement(31171..31221)
                     /locus_tag="OQM34_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452131.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31307..31405)
                     /gene="ilvL"
                     /locus_tag="OQM34_001640"
     CDS             complement(31307..31405)
                     /gene="ilvL"
                     /locus_tag="OQM34_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1452132.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31757..33277
                     /locus_tag="OQM34_001641"
     CDS             31757..33277
                     /locus_tag="OQM34_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCS1452133.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(33302..33640)
                     /gene="maoP"
                     /locus_tag="OQM34_001642"
     CDS             complement(33302..33640)
                     /gene="maoP"
                     /locus_tag="OQM34_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1452134.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33759..34598
                     /gene="hdfR"
                     /locus_tag="OQM34_001643"
     CDS             33759..34598
                     /gene="hdfR"
                     /locus_tag="OQM34_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1452135.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34694..34769)
                     /locus_tag="OQM34_001644"
     tRNA            complement(34694..34769)
                     /locus_tag="OQM34_001644"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34734..34736),aa:Trp,seq:cca)
BASE COUNT         8478 a         9518 c         8783 g         7996 t
ORIGIN      
        1 tgtcacaact tctaataatg gggtggctaa tgggattcga acccacgaca actggaatca
       61 caatccaggg ctctaccaac tgagctatag ccaccactac aaatcttgtt acgcggtatt
      121 actaccaccg cagctcaagc gccgggacta aatggcgcgc ccgacaggat tcgaacctga
      181 gacctctgcc tccggagggc agcgctctat ccagctgagc tacgggcgct tagcgccgtt
      241 gcgggggtgg ataatacgga cttcccaccc ctctgtctag tcccttttta aataaattgc
      301 tcgtttggtt acggtttgtg cgttttgctg cttattcctc cagtttatgc gctttaccgt
      361 ggcgattaag cccaaaaact ttataaatcg ccgtcaccgc cagcataaag atgatgccga
      421 caaacagcga catacgcgta tcttcattaa agtacatgcc gatcaaaacg caaatcagga
      481 atgccattgt tacgtagttg gcccacggga acaggattga gcggaacgga tggctggcaa
      541 tcgccgcttt atgtgcacgc cgaaaacgca gctggcttat caatatcaca aaccatggca
      601 ccatccccgg aagcacgctg gcactgtaga cgtagacaaa cacacgctgc ggattgggaa
      661 tgatgtagtt caggcatgag ccaattagca gaatagcaat agatactgcc acacccgcaa
      721 ccggtacgcc gtgacgggaa acttttgcca ttgccgccgg taactgacgg tttttcgcca
      781 gtgcgtagag catacgtccg caactgtaca tgccgctgtt acagccagag agcgcagccg
      841 tcagcaccac aaagttgata atgcccgccg ctgcggtaat accgattttg gcaaaagtca
      901 gtacgaacgg gctgccgttg ctgccaattt cattccacgg gaagatggtg acgataacga
      961 aaatcgcgcc tacgtagaaa atcaggatcc gccacagcac tttgcctacg gcactgcgca
     1021 gcgtcacctg cggattcttc gcttcaccgg cagtaatgcc aatcagctcc acgccctggt
     1081 aggacgccac cacaatacac agagcggtca ggaacccttt ccagccaccc gcaaagaaac
     1141 cgccatgctc tgtgagattg ctaaaaccaa tcgactgccc gccattgcca aagccaaaga
     1201 aaatcacgcc caggccaatg acaatcatca cgataatcgt ggtgactttg atcatcgcga
     1261 accagaactc gatttcgccg tacaaccgca ccgccgccag attcgccaac gccaccagcg
     1321 ccaccgcgat caatgcgggt atccactgcg ccatctccgg ggaccaaaac tggacataaa
     1381 cgccaatggc ggtgatttct gagatcccca ccgccatcca cataaaccag taagaccagg
     1441 cggtgagata gccaaaaaac gggctcatat aacgatgcgc ataaacggcg aacgaaccgg
     1501 taaccggttc gaggaacaac atttcgccca ttgaacgcat gatgaaaaaa acgaacagcc
     1561 cggcgatgat ataggccaac aatacggatg gcccggccca tttcagggta ctggcggccc
     1621 ccataaacag gccgacgcca atggtgcccc ccagggcgat gagttcgata tgtcgagctt
     1681 ccagcccacg ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat
     1741 gctttccggt gttaccggtt atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa
     1801 ttcaggagaa atggcaaata aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca
     1861 gacaggcgac gaagtgacca ctccgtcgct ttacaaagag aggaaaatca gaggttgccg
     1921 gtgtagtgcc agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc
     1981 gaaagcaggc ggtagagcca ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt
     2041 acgtgaccgg tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc atctggatgc
     2101 accagacggc agtcgcgcat gaagatctcc tgctttggcg atcccatcgc aacggtgacg
     2161 atttgcgctc cgctggcatg aatgcgttca aacagcgcct gacgctgctc gggtttaaaa
     2221 taaccatcct gactgccaac gatattcaca ttccattggt tgcgcagttt agcttcagtt
     2281 tgcgccagca cttcaggttt accgcccaca agaaataccg gcgtcccttc tttgcctgcg
     2341 cgcgccatca gctcttccca gagatcggca ccggcaacgc gggaaacctg cgcctgcggg
     2401 tactttttac gtactgaacg tacaacgctg atgccatccg catatttaaa ttcggcagcg
     2461 ttaattaact ccctgacctc ggcgttatct tcaatggtca gcattttttc ggcattaatg
     2521 gcaaccagcg ttccctgctt aagctgcccg tcagcaaaca gataatcgag ggcatgctgc
     2581 atatcacgcc aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg
     2641 ttattcattg ttatccttca acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga
     2701 gtccggcgct ttcaaaaagc cagtacaaca gttttgcgat catcagacat gcgccgaaga
     2761 ccacgataaa aaagactacg cgtgagacaa acgaatccag cccttcacgt gccagcacga
     2821 tcatattgaa gatcgcccca aagcagaaac tgtgcaatat cgcagcctta tagcgattag
     2881 tctcgcggtt gcccagctca tacagccagt cgaaccattt gatgatcagt ccaaccacaa
     2941 tcgccccgag tgggatgaac aacgcgccgc ccatcaccac cagtgagcct ataagcgtag
     3001 gcgagatcgc cagtccggag tggttattca gtacttccca ggtaaagtaa ttggctgagt
     3061 tcagcaccat actcgggcgc cccggccaca gccaggaagg gataaagaca tagaaatcgc
     3121 ggacaattgg agccaggccc tggaagtcga tgttgtcgta gttctgcaac agcaacgcca
     3181 gattctccca cggtgaaaag gtgtcgcgag tgagatagag aaacgtatag aacgcttcat
     3241 cgccgctcac attcattcca tagcgtttta acgccagcca gaacatgcca acaatcccca
     3301 gcacgcccgc cgccgccagc atccacaacg aaatccagcc gcgaataatg ccaataaaca
     3361 ggaagatagc gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc atataagtca
     3421 gcaagccaaa ggcgaccgtg ctgacgagga aaaacagcca cgctttgctg tcctggcgca
     3481 gaaagtagac caccagcatc gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc
     3541 cggagacttc actggaaaag atctgactgt aggagttaag ccggaacagc aaaaagccgt
     3601 tgtgcatgaa gaagatgccg acgcttacca gcgcgatacc catcaggatc acccacgtaa
     3661 gattggtctc cacgcggttc atggtaaaca gcggacggcg cggtacatca gcaacgcgtt
     3721 tgcgtaggcg ggttttgtag gtgacatagt aaaccgcgta gaagcagccc gcagaaagca
     3781 acacctgcaa caagatttct ggcggcgcga caccaacatc aaagcgaaat accagcacgc
     3841 tggtcagcgg gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag aagacattga
     3901 agttaaagcg cacacggcga aactcaaacc aggtcagcgt ggcaataaac agcgtgcaga
     3961 gcagccagac cacaaacagg ccactgaatt gcagcagact catgcgacct ccccggcggc
     4021 aatcgccaac gcccgctgcc agccttgtag atagttaggg ctaaagaagg caatggtgtt
     4081 tttatccgcc gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc
     4141 ggtagtaaac agcactggca aatgctgttc cgtcatatcc tgccagaacg gattttccct
     4201 gttaagcaca caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa tgccctgctg
     4261 gcgggcaaaa ataaagtaac caagatcgca ctgacgaagt agcgccagat aggcgtcaaa
     4321 ttccagtttt tcgctcaaaa tttgcagatt ttcttcgctg aataactcca gccccgcctg
     4381 acgaacttcc tcaatgtacg cttcgttatt aggcggatat cccatcggca ccaccacttt
     4441 taccgtatcg ccaaattgct gatgaacggc acgtaaagcg gcgacatgct cattgctgcg
     4501 gtcgccggag ttccccacca gaatggtcat ttttccttca cgttgccgat cgttcgccat
     4561 cgtattgaga gaagggtcca tccgcgtcgg gaagtacagc agttctcccc gcacctttgg
     4621 gtgcgttttg gcaaaaaagc tcaaatcgcc gcgggtggca aatacacagc cgactcgctt
     4681 ttgcgccagg cgacgtagtg ggtaaaaaag cttatatctc aagccactgg aaagctcata
     4741 caggtctgcc ccccagatat gccagaaaaa ctggctgggc ttaatgccac cactcagcag
     4801 agccagccac agtgtgggat tgaactgacc gtggaaaaaa aatcgctgct gacggttagc
     4861 ttttgctttc gcgatgaccg cttccgccag cgattttttc ccagggaaaa attgcacaga
     4921 aagcgccgga cagctatcac ttaagccgtc gtccttgcca acaaccataa actcgcgcgc
     4981 gtgctcgctc gtcgcggcca gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg
     5041 agggatatcc gatcccagta cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa
     5101 aacacgccac aacaaagaga aaaatagacg atataagttg ccatatatgc ctgcgccgcg
     5161 cccagtgcac catgcgcagg gattagccag tgggcaaata ccatcaataa agtgaactgg
     5221 ctgacttccg ccagaatata aaaccgcagt gacgctttgg cgatcaccag ataaccaaag
     5281 acataagcgc ccacttttaa cacatcaccc actaactgcc aggcaaagag atcgcgcata
     5341 gcggtaaatt tattcgacaa cagcaaccag atagcaaaat cacgcagcaa ccagacggta
     5401 aaactcgctg ccgccactgc cggtaagacg aatttcagcg atttaaccac ttcccgggtg
     5461 atatcgcgct tttccgttag ccgcgacaac gtgggcagca aatatacgct gaacgatgcc
     5521 gtaataaatt gcaggtaggc atcggaaata ctgctcaccc cttgccagat ccccacttca
     5581 tcccagctat actgcgccgc cagcagttta cgcatcatga tgtaagcaac aggcaaggtc
     5641 accgacgtaa tcaacgccat gagcgtaaat ttgctcaact gccctgccag accgttatcc
     5701 cagctgggtt tcagatagct taacgggatg acaccacgtt taatcaacat gatggcggca
     5761 ggaattacca ccagcgcggg aatcagcgcc agacccagca acgccccttc ataaccgccc
     5821 aaacggtaac tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat cagcgataac
     5881 gcattacctg cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt gccccaggcg
     5941 atccccattt gcaccagcgc cactaaacgc accagcccct gatagtcggt attaccaaac
     6001 aatccctggc tgattggcgc agctgccagc acaaaaacca gcgccatcag cgtagagaaa
     6061 ccaagtacca tcgctgatga agtgccgacc acgcggcgca gctgttgcgg attatcatgg
     6121 tactgggcaa cgtatttggt tacaccgtta aagatgccag ccccggcaag cacgccgagc
     6181 acggtaatca actggcggaa atttgccgcc agcccaagcc ccgccggacc aaatgacacc
     6241 gccagcaact taccgaccag taacccggca ccaatcttga ccagtgtact ggccgccgtc
     6301 cacaaggacg cttttgccaa cgacatatca ggagaagtag ttcaacaaag tcgcaattac
     6361 cgtacgctga ttgacgggcg acaggttgta gaacagcggc aggcgcagca ggcgctcgct
     6421 ctctttggtg gtgtagcgat cttcaccgtg gaactcacca aagtgttccc ccgcagggca
     6481 accgtgcagc ggaatgtaat gaaacaccgc catgatttcc gcttctttca gaaagttaat
     6541 caacgcgctc cggtcatcaa tatcccgcag tttaatgtag aacatatgcg cgttctgcac
     6601 gcagccatcg ggaatcgacg gcagctcgat acgcccggct ttcgccagag gcgctaacgt
     6661 atcgtagtag ttttgccaca gcgccagacg ttgctggttg atacgatccg ctgcttccag
     6721 ttgcgcccac aggtacgcag cttgcagatc ggacatcaaa tagctggagc caatatcgcg
     6781 ccaggtatat ttatcgacct gaccacggaa gaactggctg cggttagtgc ccttttcacg
     6841 gatgatctcg gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc
     6901 acccgccgtg tagtttttgg tttcatggaa gctaaagcag ccaatatgac caatggttcc
     6961 cagtgcacgc cctttgtaag tggacatcac gccctgagcg gcatcttcca ccacaaacaa
     7021 attatgcttt ttcgccaacg ccataatggt gtccatttcg caggccacac ccgcgtaatg
     7081 gaccggcacg ataacgcgcg ttttgtcggt gatcgctgct tcaatcaacg tttcgtcgat
     7141 gttcatggtg tccgggcgaa catccacaaa gacgattttc gcgccacgca gcacaaaggc
     7201 attggcggtg gagacaaagg tgtagctcgg catgatcact tcatcgccag gctggatatc
     7261 gagcagcagc gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta acactttggc
     7321 gctgccaaaa cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc catcgccaca
     7381 cagtttgccg ctacccattg ccgactgcat atagtcgagt tcggttccca ccaccggcgg
     7441 tgcgttaaat ggaatcatgt gatcacctgt ataaccagta cgcggtgctt tctacattcg
     7501 caccactttg tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc gccacccgca
     7561 aagttgtttt accgcgagca tacgcccagt ttagcgccgt ttgcatcagc tcagcacctg
     7621 caccgcgtcc agccagcagg ccaattcgcg catctgtcgc attgagttcc cgtaaagaga
     7681 catagccgcg aatatcgccg gacgccgcac gtagaatcag acattgatga tcaaaggtgc
     7741 cgcgcacggc attttcaatc cactgtgcat aaaagcgacc gctggcgtca ggcgcatacc
     7801 acggcgcacg aaaacggctt tgcgcaaatg cggcgctagc taactgacgt aatgcgggaa
     7861 tatcggtctc ttgtgccacg acagcaccgc tatcactgac attgttcacg ggtagcgcca
     7921 aatcaacttc accttctacc agggagaatc ccagctgttg cagggcatcc agttcccccg
     7981 tatttgatgc cgcaattttg gcctgcaccc gtgaccatgg cgctaaggcg tctgacgtca
     8041 ggagcggtgc ttcagacgta atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt
     8101 cccaggttag tggctcaata ctggcgcgga cgggcacgaa gtaactccag cagatattgg
     8161 ccgtagccag ttttcgctaa tgaactggca gcacgcttca caccctcgtc atcgagccag
     8221 ccgttacgcc aggcgatctc ttccaggcag gcaatcttaa agccctggcg tttttctact
     8281 gtctgcacaa aagtgctggc ctcaatcagg ctgtcgtgag tgccggtatc cagccaggca
     8341 aatccgcgcc cgagcagttc aacggtcagg ttgcccgcct cgaggtacat ctggttgatg
     8401 gaggtaatct ccagttcacc gcgctccgac ggcttcacct gctttgcgta ctccacgact
     8461 ttactgtcgt agaaataaag cccggtcacc gcccagtttg acttcggctg ttttggcttt
     8521 tcttcaagcg agatagcgcg gaaattgtcg tcaaattcca ccacgccaaa gcgttccggg
     8581 tccatcacct gatagccaaa aaccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc
     8641 ttcggactga agccctgacc gaagaagatg ttatcgccca gcaccagaca agaaggttca
     8701 ccattaagga aggtttcacc gatgataaag gcctgcgcca gaccgtccgg gctgggctgt
     8761 tcggcatatt ccagctggat accgaactca ctaccatcgc ccagcaggcg ctggaaataa
     8821 cctttatctt ccggcgtagt gatgatgaga atttcgcgga taccggccag catcagcacc
     8881 gacagcggat agtaaatcat tggcttatcg taaatcggca acagttgctt cgatacgccg
     8941 cgcgtaatcg gatgcaatcg ggtaccggaa ccgcccgcca ggataatacc cttcatgttt
     9001 gcctccgctg aaaattagcc tttcagacct aaacgctcac cctgatagct gccgtcctgc
     9061 acctgcttcc accagctttc attagccaga taccactgaa ccgttttacg cattccactt
     9121 tcaaaggttt cctgcggcag ccagccaagt tcacgggcaa ttttcgaagc atcaatggca
     9181 taacgcagat catgccccgg acggtcagcg acaaaggtga tcaggtcacg atagtgcgcc
     9241 acgccgtgcg gcttgttcga agccagttct tccagcagct cgcaaatggt ttccacaaca
     9301 tcgagattct tacgctcgtt gtgaccacca atattatagg tttcaccgac tttcccggtg
     9361 gtcgccacgc aatacagcgc gcgggcgtga tcttccacat acagccagtc acggatttgc
     9421 tgcccgttgc catataccgg cagcggttta cccgccagcg cgttgaggat catcagcggg
     9481 atcagttttt ccggaaagtg gtaaggaccg tagttattcg agcagttggt gatcagcgtc
     9541 ggcagaccgt aggtccgcag ccaggcgcgc accagatggt cgctgctggc ttttgacgcg
     9601 gaataagggc tgctcggcgc atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc
     9661 aggtcaccat atacttcgtc ggtggagata tgatgaaaac ggaacgctga ttttttatct
     9721 tccgtcagcg tattccagta agcccgcgcc gcttcaagca atgtataagt cccgacaatg
     9781 ttggtttcaa taaacgctgc cgggccgtca atagaacggt caacatggct ttcggctgcc
     9841 agatgcatga cacagtctgg ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg
     9901 cagatatcaa ctttctcaaa ggcaaagcgc tcgctttgcg cgaccggtgc cagcgacatc
     9961 aggtttccgg cgtaggtcag cttatcgacc actaccaccg catcgctcgt ttcgttgatg
    10021 atataacgca ccagcgccga gccaataaac ccggcaccac ctgttatcag aatttttctc
    10081 atcagcgcca gactcctttg gcatcgacga catactgctg atggacattg tcgccattga
    10141 taactttgaa ctgactatga tcgaccagca taaccagcac gtctgccgtt gccagcgcct
    10201 cgtcaagctg cgccagagta caaagcccgg tcagtttttt cggcaactgg tggatgttag
    10261 gctcaacaac cagcgtttcg ccgctgtgcc actgggcgat cagttcagcg atttccatcg
    10321 ccgggctttc gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag caggcgattt
    10381 tcagttcact ggcgcgttta tcggtagcag caaggcagtc ggcaaccgcc gctttcacct
    10441 gatcgataac ccagaacggt ttgtgatcgt taacttcgcg cgcggtacgg ataagccgcg
    10501 cctgctgggg gttctgtgcc acgataaacc acggatcaac agcaatgcag tgaccgccca
    10561 cgccagggcc aggctgaaga atattaacgc gagggtgacg attcgccagg cgaatcagtt
    10621 cccagacgtt aatcccctga tcggcacaaa tcagcgacaa ttcattagca aaagcgatat
    10681 tcacatcgcg gaagctgttt tcggtgagct tacacatttc cgccgtccgc gagttagtga
    10741 cgacacactc accttcgagg aaaattttgt acaattcgct ggcgcgggcc gaacaaaccg
    10801 gcgtcatacc accaatcacg cgatcgtttt taatcagctc gaccataacc tggcccggta
    10861 atacacgttc cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga
    10921 aagtcagatc cgggcgcatc tctgctaacc attctgccat cttctcggtt gaccccaccg
    10981 gcgaggtgga ttcaaggatc accagcgcgc cttttttcag cactggcgca atggagcgag
    11041 cagccgattc aacgtaggtc atatctggct catgatcgcc tttaaacggc gtaggtacag
    11101 caatcagcca ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta
    11161 cggcagtttt tactacgctc gccaaatcag gctcgacgat atggatttcg ccacgattga
    11221 tggtatcaac cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa
    11281 acgctgctgc tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac
    11341 tcatagtgat atccgattat tttttaacgc ttccagaatg cgagagcatg cctgaccatc
    11401 accatacggg ttatgggcgc ggctcatagc ttgatattcg ttttcgtctt ttaaaagacg
    11461 cgtcacttcc tcgacaattc gctgcttatc cgtgcctacc agacgcaccg tacccgccgt
    11521 caccgcttcc ggacgctcag tggtatcgcg catcaccagc accggtttcc ccagcgaagg
    11581 cgcttcttcc tgaatgccgc ctgagtcagt caaaatcagc caggcgtggt tcatcagcca
    11641 gacaaacggt aaatactcct ggggatcgat cagaatgaca tttttcacat gccccagaat
    11701 gcgattgacc ggctctctga cgttcgggtt gagatgcacc ggatagacaa tctggatgtc
    11761 ctggtgcgtg gtggcgatgt ctgccagcgc gtggcagatt tcttcaaagc cacgaccgaa
    11821 actctcacgc ctatgaccgg tcaccagaat catcttttta tcgggggcga taaacgggta
    11881 atttgccgcc agttctgaac gcagcgtgtc gctgctcatc acctggtcac gcacccataa
    11941 cagtgcatca atgactgtat taccggtaat gaagattcgg ctatccgcaa cgttttcacg
    12001 cagcaagttt tgccgggaag tttcggttgg agagaagtga tacatcgcca gatgcccggt
    12061 caatgtacgg ttagcctctt ccggccacgg cgaatagaga gcgcccgtgc gcagaccagc
    12121 ctcaacgtga ccaacaggaa tacgctgata aaacgccgcc aggctggttg ccagcgtcgt
    12181 cgtcgtatcg ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa taggttttag
    12241 cccttccaga atccgacagg ttatctctgt caggccctgt cctggctgca ttatgttgag
    12301 atcgtagtca ggtacaatgg aaaagagttt cagtacctga tcaagcatct cccgatgctg
    12361 cgcagtgacg caaactttag cctcaaaaaa aggatctttt gccaacgcat gcaccaacgg
    12421 agccatcttg atggcttccg ggcgcgtacc aaatacagtc agtactttca catcgattct
    12481 cttcgaataa gcggcgagcg cctttgcgct caccgcagca gtgttgctat ttcgagcaac
    12541 ggcgggttaa tgcgacacca gccccgatca gccccccgac aatgccccac ataatcatca
    12601 ggaaggcacg acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat
    12661 aggtctgaaa acgcggatcc agggttggac caacattcag ggtgtttaac atggctcgat
    12721 tctgatcata gtcgagatca aaggccggac cgacggcctg taaattttcc agtcgagcct
    12781 ggagcattgg acgcccaagc aggaacattt ctgaatcagg taattcctcg gcaggcacat
    12841 ctgtcgcact gcgcgaaata ttatgctgct cagcaatttt cagcgcctgc tcaatactgt
    12901 tcatccggcg gtcgtagatg gctttcgcca cctcttcctg acgcttcacc tgagctttca
    12961 tctggatggt gcgtgccgcc catgcgcctt tcagctcatc attcagatgg ctggctgcac
    13021 gctggctggc aaaagcaaca tactgacgta acaggttatt agcgtcaggc gcggtttcgg
    13081 caataagctt cacgctgtca ttgaccgcgc gagtaaagtc tccggggata aactggatgt
    13141 tgttaatcat ttcatccagc aacgccgcat cggctttgct gttgcccacc atccgctgtt
    13201 tgtaatagtc ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc agttgcataa
    13261 caaactcttt ataggcttcg tccatgaccg atggttgatc ggcagaagcc atgtttgaac
    13321 ggacatccag gttacgcaaa aattgctgct gcgagtaata tccccccagc atattcaccg
    13381 ttggacgatc ggtaatcgcc gtcgagctcc actcctgacg agcaaaaaaa gtatacgcca
    13441 gcgcgattaa cgcaaacgcc agccccatgc caataatcca tagcttccca gcccacaagg
    13501 tacgaaacaa cccacgaata tccagttcat tttcagcgtc ttcggccggt ttcccaggca
    13561 ttggttgtgt catcacatcc tcatttattt ggttaaattg gggctgccac cacgatttct
    13621 acgcagtctg cgttttacgc gcttaataaa gcgagcaact ttccaggcac gcttaatgca
    13681 atatccatag aggaagaatg ctagcaaaaa gagcaccagc atgacccact ccgggacaaa
    13741 atgagaatat tctgccagca cgccaatgga agcgagcagt gctgcggcaa gggtaatcag
    13801 cacaaacgcc tgacgggaag taaacccggc acgcatgatc aaatggtgaa tatgctgacg
    13861 gtcaggagag aatgggctca tgcctttacg caggcgacgg tacataatcg ccaccatatc
    13921 cattagcgga atggcgatta tccacaaagc ggtaaccggg ctgatgggat gggttttgcc
    13981 ctgggtcgtt tcgagcagga tccagataac ggtaaaacca atcagcgtac tgcccgcatc
    14041 acccataaag actttgtagc ggcgacccag gataccaagg ttaagcatga tgtatggcag
    14101 gatggcggcg atcatcgcaa agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa
    14161 aatcataccg attgctgcaa acgagacgca ggacaacccg cccagcaagc catcaatgcc
    14221 atcaaccatg ttgaacgcat taatggccgc ccagacggca aatagcgtca ggaagtaacc
    14281 aaacggtccg agcaccatct cccaggagcc aaagatataa cccaggctac tgagataaag
    14341 tttgccgaac accatcataa caatgccaac agcggcctgt atggtggcac ggatttttac
    14401 gctgatatca aaacgatcat ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag
    14461 atagagagat gcatgcggaa tatagtaatc gacaattccg aacgtgaagc aaatccctgc
    14521 gtaaaccgaa atccccccaa cgagaggtat caatccctgg tgacgtttgc ggaagtttgg
    14581 tttatccact aaaccgactt tttttgccac cttacgggca aaaaacagaa acagtgtcgt
    14641 gaataaaaaa atactgatga gatcagtact cactgtcagt aaattcacaa tgcatgctct
    14701 cagagaaaat tattagcaga agtataacca cgaagacctt tattcagaag ggaaagtccg
    14761 ttccgaaccc accagccctg tgcaaaaatc aaccatttga tatgcaaatt gcataacatg
    14821 ccagcaaatt ccaggaattt tcctaacatc gccagcgcga cattaagatt acagatataa
    14881 aagcaaaacg ccacgtaaac acgtggcgtt tttggcataa gacaaattta tgagcgtttc
    14941 atcatttcga agaaatcgtc attggtcttg gtcatggcca gtttattaat gaggaattcc
    15001 attgcatcga tttcgcccat cgggtgaatg attttgcgca ggatccacat tttctgcagt
    15061 tcttcctgag tcgtgagcag ctcttcttta cgggtaccgg aacggttgta gtcgatagcc
    15121 gggaagacgc gtttttcagc gatcttacga gagaggtgca gttccatgtt gcctgtacct
    15181 ttaaactctt cgtaaataac ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg
    15241 ataatggtca ggctgccgcc ctcttccacg ttacgcgccg caccaaagaa gcgtttcgga
    15301 cgatgaaggg cattggcgtc cacaccaccg gtcaacactt tacctgacgc cggaacaaca
    15361 gtgttgtaag cgcgcgccag acgagtgatg gagtcgagca gaatgataac gtctttcttg
    15421 tgctcaacca ggcgtttggc cttctcgatc accatttccg caacctgaac gtggcgagat
    15481 gcgggttcgt caaaggtaga agcaacaact tcacctttta ccagacgctg catctcggtt
    15541 acttcttccg gacgttcgtc gatcagcaga accatcagca cacaatccgg gtggttgtaa
    15601 gcaatgctct gagcaatgtt ctgcagcagc atagttttac cggctttcgg cggtgccaca
    15661 atcagaccac gctgaccacg accgataggt gatgccagat ccagtacgcg agcagttaaa
    15721 tcttcagtag aaccgttacc acgttccata cgcagacgag agtttgcgtg cagcggggtt
    15781 aagttctcaa agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta
    15841 actttcagca gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt accagagatg
    15901 gtatcaccag tgcggaggtt gaaacggcgg atttggctag gggaaacgta gatgtcatca
    15961 ggaccggcga ggtaggagct gtctgcggaa cggaggaaac caaatccatc ctgcaatatc
    16021 tccagtacgc catcaccaaa gatatcttcg ccactctttg cgtgctgctt caggatggca
    16081 aaaataatgt cctgcttacg catgcgagcc aggttttcca gccccatatt ttcgccgaga
    16141 gtgatcagct cagaaaccgg cgtattcttt aattcggtaa gattcatagt ggtgtgagtt
    16201 cttaaacttg gggtaaatct cgaacttaat gttgtgaatg gtatggcagg atcatccatg
    16261 cctgtttacg gtcatcgact catgtctttt cgctgcctgg tcacaggaga gtacgcagaa
    16321 ctgaaacgac aagacggatt gagtgacgag ccagaaatct gttcacttcg catttaagat
    16381 aaaacgggaa gcgttggaaa ttacaagatt caaacttaat aaggtatgtt taatacgaag
    16441 tcaacactaa gttagcatga ctcacgccgg gcgtccagtt tttagcgacg gggcacccga
    16501 acatgaaatt cccttacgcc aggttagcgt cgaggaactc tttcaactga cctttagaca
    16561 gtgcgcccac tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac
    16621 cacggatgcc atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg
    16681 tcagtttgcc ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg
    16741 gaccgcacca ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg
    16801 tgtcaaaact gtcgtcagtc aggtgaataa ttttatcgct catatataac tccacaggaa
    16861 taagcctggc gtgttggttt cgttgttggt gtaacattaa ccaactaaag gttgacttta
    16921 tttcaccgga tacgctttcg taaagcaata gtaagctgat attctaccac actatgagca
    16981 aaacacattt aacagaacag aagttttccg acttcgccct gcatccgaag gttgtagaag
    17041 cccttgaaaa aaaagggttt cataactgta cgcccattca ggcactggcc cttccgctga
    17101 cgctggcggg tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt
    17161 ttcttacgtc aacgtttcat tatcttctct ctcatcctgc gattgccgat cgcaaggtga
    17221 atcaaccgcg tgccttaatt atggcaccga cgcgtgaact tgccgtgcag atccacgccg
    17281 acgcggaacc gctggcggaa gctaccggcc tgaagctggg cctggcttac ggtggtgatg
    17341 gctatgacaa acagctgaaa gtgctggaaa gcggcgttga cattctgatt ggcaccacgg
    17401 gtcgtttaat tgactacgcc aagcagaacc acattaacct cggtgccatt caggtagtgg
    17461 tactggacga agccgatcgc atgtacgatc taggctttat taaagatatc cgctggctgt
    17521 tccgccgtat gccgcctgca aatcagcgcc tcaacatgct gttctccgcc acgctttcgt
    17581 accgggtacg tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac
    17641 cggaacagaa aacgggccac cgtattaaag aagagctttt ctacccttct aacgaagaaa
    17701 aaatgcgttt gctgcaaacg ctgatcgaag aagagtggcc agacagagcg attattttcg
    17761 ccaataccaa acaccgttgt gaagagatct ggggccacct ggcggcagat ggtcatcgtg
    17821 tcggtttgtt gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta
    17881 cccgtggcga tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc
    17941 cggcagtgac gcacgtcttt aactacgatt tacccgatga ctgtgaagat tacgttcacc
    18001 gtattggtcg tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag
    18061 agtatgcatt gaatttgcct gctattgaga cctatattgg tcactcaatt ccggtaagca
    18121 aatacaatcc ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc acgcgcccgc
    18181 gcacaggcaa tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa
    18241 taaaatgtca tactcaaaat cattcaaact gtatcaagat ggcaagagaa tgaatcccga
    18301 tgagcttact gtagtaagtg attcgggtga aagaatgcag ccaacacaga gacagattga
    18361 aggatgaaga gtatgggttc cacctcgtcg ctgtatgcag ccattgatct cggttcgaat
    18421 agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct gacgcgaatt
    18481 aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgacctctc taatgaagca
    18541 atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga tatccctccc
    18601 tcgcaaattc gcgttgtcgc tacggcgaca ttacgacttg ccgtcaatgc gggtgatttt
    18661 attgccaaag cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg tgaagaggaa
    18721 gcgcgtctga tttatcaggg cgttgctcac accactggtg gtgccgatca gcgcctggtg
    18781 gtggatatag gcggtgccag tactgaactg gtaaccggca cgggtgcaca aaccacctcg
    18841 ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc cgatcgtaat
    18901 ctggggcaag aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt acgtccggtt
    18961 gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag
    19021 gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct ggaaaagttg
    19081 cagcaattga aacagcgagc cattcattgc ggtcggctgg aggaactgga gattgacggg
    19141 ctgacgctgg aacgtgcatt agtgttcccg agtggtctgg cgatcctgat cgccattttt
    19201 accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc
    19261 tacggcatgt tgcatctggc cgtcgagcag gatattcgca gccgtacact gcgtaatatt
    19321 caacgccgct ttatgatcga tattgatcag gcacagcgcg tagccaaagt tgcggctaac
    19381 ttcttcgatc aggtggaaaa cgaatggcat cttgaagcaa taagccgcga tttgctcatc
    19441 agcgcctgtc agcttcatga aatcggcctg agcgttgact tcaaacaagc tccgcaacac
    19501 gctgcttatc tggtgcgtaa cctggatctt cccggtttta cccccgcaca gaagaaactg
    19561 ctggcgacgc tactgctcaa ccagactaac ccggtcgatc tctcatcgct gcatcagcaa
    19621 aatgccgtac caccgcgcgt cgcagaacaa ctctgccgtt tactccgcct ggctatcatt
    19681 tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc taaccatgaa
    19741 ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg taaagagatt
    19801 attgctcagg aaaaccagtg gcagagctat gtccactggc cgctggaagt gcattaatga
    19861 gtaagtgccg gatgcgatgc tgacgcatct tctccggcct tgattatttc cctcgttttg
    19921 ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc cgttcttcgg
    19981 cactgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa
    20041 agcggctcgg ctccgggcgc accagttcgc cgtactgacg gcgttcttta cacagcgtaa
    20101 aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc tcctcatcga
    20161 tattatcttc atcgatactg ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt
    20221 agacataagg aaactccagc ccttttgacg cgtggagagt catcagttgc acctgatcca
    20281 gctcttcttc actctcacca cgctccatca tgtcgcgtaa agtaaagcgc gtcaccacct
    20341 gggtgagcgt catcggctca tccagttcgc tgccttccag catctccgtc atccagctaa
    20401 acagttggtt gacgttcttc atgcgcattt cggcagcttt cgtgctgggc gatgtttcgt
    20461 acagccagga ttcataatcc atgccgtgga tcagatcgcg caccgcggca atcggctccc
    20521 gctccgccag acgctggatt tctgccaacc agtgagtgaa gcgggtcaat gcttcataac
    20581 cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac atgcttttat
    20641 tgcgcgtcat cgcccactca cccagctttt tcagcgtcgc cgggccaatc tctcgcttcg
    20701 gcgtgttaac gatacgcaga aatgcgctgt catcgtccgg gttagtcagc acgcgcagat
    20761 aagccagcaa gtccttgatt tcaggacgag agaaaaacga cgtaccacca gaaattttgt
    20821 acgggatgcg gttttgcatc aggaattttt caaacacccg cgactgatag ttaccgcgat
    20881 aaagaatagc gtaatctttg tactgcgttt tattgacgaa gtgatgcgca atcagttcac
    20941 cagtgacgcg ctcggcttca tgttcttcgt tattcgcgct taaaactttt agctctgtgc
    21001 cgtaacccag ttcggagaac agacgctttt caaagacgtg cgggttattg gcgatcagga
    21061 tgttcgccgc tttcaaaata cgtccggaag agcggtagtt ctgctcaagc ttaatcacct
    21121 tcagcgccgg aaaatcctgg ctcagcagca ccaggttttg cggacgtgcg ccgcgccagg
    21181 agtagatcga ctggtcatcg tcacccacca cggtaaagcg cgcgcggctg cccaccagca
    21241 gtttcaccag ttcatactgg ctggtgttgg tgtcctgata ttcgtccacc agcaggtagc
    21301 gaattttgtt ctgccagcgc tcgcggactt cttcattgcg ttgcagaagt agcgtcggca
    21361 ataaaatcag atcatcgaag tcgagcacat tacaggcttt caggtgtgca tcatacagcc
    21421 cataacaatg ggcaaaaata cggtcccgct cgccaatcgc actggctgcc gcctgggacg
    21481 gtgttttgag atcgttcttc cagttagaga tggtcgaaat caactgttgc aggagaactt
    21541 tgtcatcttc aatcagccct tcggtcaact ctttgagcaa cgccagctga tcggtatcgt
    21601 caaacagcga aaagttcgcc ttcatcccca gcgccgcata ctcacgcttg atgatatcca
    21661 gccccagcgt gtggaaggtt gagatcatca gcccatgcgc ctctttgcga cccagcgtct
    21721 gtccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc gccgcgatat
    21781 gtcgcgcctg ataaccgcac ccgcggatca gatgggcgat tttattggtg ataacacgag
    21841 ttttaccgga acccgcaccc gccagcacca ggcaggggcc ggtaacgaat tcgacagctt
    21901 gttgttggcc ggggtttaga cgcataggaa aatcgctcaa tgaaagtcgg gaggggaaaa
    21961 taacagcgtg gtagtatagc cagcctcatc cttaccactc aaggcacgat catggcaaaa
    22021 acagcagcag cactgcatat ccttgtaaaa gaagagaaac tggctctgga tcttctcgtg
    22081 cagattaaga acggtgccga tttcggcaag ctggcgaaga aacactccat ttgcccatca
    22141 ggcaaacgcg gcggtgattt aggtgaattc cgccagggtc agatggttcc ggcgttcgat
    22201 aaagttgtgt tctcttgtcc ggtactggag ccaaccggcc cgctgcacac ccagttcggt
    22261 taccacatca ttaaggtgct gtaccgtaac taatagcaag gccttctccg ggagaaggcc
    22321 ttgaactctg aatcgctatt aacccgcaac agcaatacgt ttcatatccg tcatatagcc
    22381 gcgcagtttc ttacctactt gctcaatcgc atggctgcga atcgcttcat ttacatcacg
    22441 cagctgcgcg ttatctaccg caccttccgg aatagcttta cccaaatcgc ccggttgcag
    22501 ttctgccata aacggtttca gcagcggcac acaagcgtaa gagaacagat agttaccgta
    22561 ctcggcagta tcagagataa ccacgttcat ttcatacaga cgcttacggg cgatggtgtt
    22621 ggcaatcagc ggcagttcgt gcagtgattc atagtaagca gattcttcga tgatgccgga
    22681 atcaaccatg gtttcgaacg ccagttcaac gcccgctttc accatcgcga tcatcagtac
    22741 gcctttatcg aagtactcct gctcgccgat tttgccttca tactgcggcg cggtttcgaa
    22801 ggcggttttg ccggtctctt cacgccaggt cagcagtttc ttatcatcgt tggcccagtc
    22861 cgccatcata ccggaggaga attcgccgga gatgatgtcg tccatatgtt tctggaacag
    22921 cggtgccatg atctctttca gctgttcaga caacgcgtaa gcacgcagtt tcgccgggtt
    22981 agagagacgg tccatcatca gtgtgatacc gccctgcttc agcgcttcgg tgatggtttc
    23041 ccaaccgaac tgaattagtt tttctgcgta tgccggatcg gtgccctctt ccaccagctt
    23101 gtcgaagcac agcagcgagc cagcctgcaa cataccgcac aggatggttt gctcacccat
    23161 caggtcagat ttcacttccg caacgaagga cgattccagc acgcccgcac ggtgaccacc
    23221 ggttgctgcc gcccatgctt tagcgatcgc catgccttcg cctttcggat cgttttccgg
    23281 gtgaacggca atcagcgtcg gtacgccgaa tccacgtttg tactcttcac gcacttcggt
    23341 gccagggcat ttcggcgcaa ccatgactac ggtgatgtct ttacggatct gctcgcccac
    23401 ttcgacgatg ttgaaaccgt gcgagtaacc cagcgccgcg ccgtctttca tcagtggctg
    23461 tacggtgcgc actacatcag agtgctgctt gtccggcgtc aggttaacca ccagatccgc
    23521 ctgtggaatc agttcttcgt aagtacccac tttaaaacca ttttcggtcg ctttacgcca
    23581 ggacgcgcgc ttctcggcaa tcgcttcttt acgcagagcg taggagatat cgagaccaga
    23641 atcacgcatg ttcagaccct ggttcagacc ttgtgcgcca cagccgacga tgactacttt
    23701 tttaccctga aggtagctcg cgccatcggc gaattcatcg cgccccataa agcgacattt
    23761 gcccagctgt gccagctgct gacgcagatt cagtgtattg aagtagttag ccatggtgat
    23821 tcctcgtgat gttgtgcttc ttattgttcg gttcgcgttt gcgagattga attcactata
    23881 tgacaggaaa tttattgcgg aaattgatat attcacaacg tcacattgca atttctgcaa
    23941 cgtcaacatt gagggctgcg ccattggatt tacgcgatct gaaaaccttc ctgcatctgg
    24001 cggaaagccg ccattttggc cgcagcgcgc gggcgatgca cgttagcccg tccacgctct
    24061 cacggcagat tcagcgcctg gaagaagatc tcggccagcc gctgtttgtg cgcgataacc
    24121 gcacggtgac gctgactgaa gcgggcgaag aactgcgcgt tttcgcccag caaacgctgt
    24181 tgcagtatca gcagctgcgc cacaccatcg accagcaagg gccgttgctc tatggcgaat
    24241 tacatatctt ctgctcggtg accgccgctt acagccatct gccgccgatt ctggatcgct
    24301 tccgcgcgga acacccgtcg gtggagatca aactcactac cggtgatgcg gcagatgcaa
    24361 tggaaaaagt cgtcacaggt gaagctgatc tggcgattgc cgggaagcca gagactctgc
    24421 ccggcgcggt ggcgttttcg atgttggaga atctggcagt ggtgttgatt gcacccgcat
    24481 tgccctgccc ggtgcgtaat caggtttctg tcgaaaagcc ggactggtca acggtaccgt
    24541 ttatcatggc cgatcagggg ccggtgcgcc gccgcattga gttgtggttc cgacgcaata
    24601 aaatcagtaa cccgatgatt tacgccacgg ttggcgggca tgaagcgatg gtatcgatgg
    24661 tggcgctcgg ctgtggcgtg gcattgttgc cggaagtggt gctggaaaac agccccgaac
    24721 cggtgcgtaa ccgcgtgatg attttagagc gcagcgatga gaaaacgccg tttgagctgg
    24781 gcgtgtgtgc gcagaaaaag cggctacatg agccgctaat tgaggcattc tggaagattt
    24841 tgccgaacca ttaacccgcc aaaaagaacc tgaacgccgg gttattggtt tcgtcgtggc
    24901 aatcgtagcc cagctcattc agccgggttt cgaaatccgg ttcatggtcg ccaagttcga
    24961 acgccgccag tacgcgcccg tagtcggtgc catggctgcg atagtggaac aaagaaatgt
    25021 tccagtgcgt acccagcgtg ttgaggaagc gcagcagcgc gcccggtgat tccgggaatt
    25081 cgaagctgta gaggcgttcc tgcaacggat gtgacggacg cccgccgacc atatagcgca
    25141 catgcaactt cgccatttcg tcgtcggaga gatccaccac gctgtagccg ccgtcgttga
    25201 gcatctgcaa aatttctttg cgctcttcga ggccgcggct caggcgcacg ccgacaaaga
    25261 tgcaggcgtt tttggcatcg gcaaaacggt agttgaactc ggtgaccgat cgcccgccaa
    25321 gcagttggca gaatttgagg aagctgcctt tttcttccgg aatggtcacc gccaacaacg
    25381 cttcacgctg ttcgcccagt tcgcagcgtt ccgagacgta gcgcaggccg tggaagttca
    25441 cgttggcacc ggaaagaata tgcgccagcc gttcgccgcg aatgttgtgc tgggcgatat
    25501 attttttcat tcccgccagc gccagcgcgc cagagggttc cgccaccgca cgcacatctt
    25561 cgaataaatc cttcatcgcc gcacagatcg catcgctatc gacggtgatg atgtcgtcga
    25621 gatactcctg gcataaacgg aaggtttcgt caccgatgcg ttttaccgcg acaccttcgg
    25681 cgaacagtcc cacgcgtggc agatcaaccg gatgacccgc atccagcgct gctttcaggc
    25741 aggcggagtc ttccgcttct acggcgatca ctttgatttg cggcatcagt tgtttgatca
    25801 gcaccgctac gcccgccgcc agaccgccgc cgccgactgg cacaaatacg cggtcgagat
    25861 gggcgtcctg ctggagcagt tccagcgcca gcgtgccttg cccggcaatc accatcggat
    25921 ggtcgaacgg cggcacccag gtgaacccct gctgctgtga cagttcgatc gctttagctt
    25981 tcgcttcgtc aaagttcgcg ccatgaagca gcacttcgcc gccgaatcca cgtaccgcat
    26041 cgactttgat gtcggcggtg gcggttggca taacgatcag ggccttcacg cctaaccgcg
    26101 cagaagaaaa cgcgacgccc tgcgcgtggt tacccgcaga agcagtgatc acgccgtgcg
    26161 ctttttgttc ttccgtcagg cccgccatca tggcgtatgc gccgcgcagc ttaaagctgt
    26221 gcactggctg gcgatcttcg cgcttcacca gaatgacgtt atcaagacgc gacgacagtt
    26281 tttccatttt ttgtagcggc gtaacctgcg ccgcctcgta aaccggcgcg cgcagcactg
    26341 ctcttaaata ttcggcacct tccggagcac cggacagggg ttgcgagtca gccattatta
    26401 accccccagt ttcgatttat cgcgcaccgc acctttgtcg gcgctggttg ccaggctggc
    26461 gtaggcgcac agggcaaagg aaacctgacg ttcacggttt ttcggcgtcc aggctttgtc
    26521 accccgagct tcctgcgctt cacgacgcgc cgccagttcg gcatcgctta cctgtaactg
    26581 aatgccacgg ttcgggatgt cgatagcgat caggtcacca tcttcaatca gaccaatgct
    26641 gccgccgctt gccgcttccg gtgagacgtg gccgatggaa agaccagagg tgccgccaga
    26701 gaaacgaccg tcggtgatca gcgcacaggc tttaccgaga cccattgatt tcaggaagct
    26761 ggttgggtag agcatttcct gcatgccagg gccgcctttc gggccttcat agcggatcac
    26821 caccacatcg cccgcgacaa ctttgccacc gaggatcgct tctaccgcgt cgtcctggct
    26881 ttcgtacact ttcgccgggc cggtgaattt gaggatgctg tcatcgacgc ccgcggtttt
    26941 cacgatgcag ccgttttcag caaaattacc gtagagcacc gccaggccgc cgtctttgct
    27001 gtaggcgtgt tccagcgagc ggatacagcc gtttgcgcga tcgtcatcga gagaatccca
    27061 acggcaatcc tgcgagaatg cctgtgtagt acgaatgccc gcagggcctg cgcggaacat
    27121 attttttacc gcgtcatcct gggtcagcat aacgtcgtat tgttccagag tttgcggcaa
    27181 cgtcaggcca agtacgtttt tcacatcacg gttcagtaac cccgcgcgat ccagttcacc
    27241 gagaataccg ataacaccac cagcacggtg aacatcttcc atatggtatt tttgggtgct
    27301 cggcgcaact ttgcataact gcgggacttt acgcgaaagt ttgtcgatat cactcatggt
    27361 gaagtcgatt tccgcttcct gcgccgccgc cagcaggtga agtaccgtgt tggtagagcc
    27421 gcccatcgcg atatccagcg tcatggcatt ttcaaacgcc gctttgctag caatattgcg
    27481 cggcagtgca ctttcgtcgt tttgctcgta gtaacgtttg gtcaattcaa caatgcgttt
    27541 accggcattg aggaacaact gcttacggtc cgcgtgggtt gccagcagcg agccgttgcc
    27601 cggctgcgac aggcccagcg cttcggtcag gcagttcatt gagttagcgg taaacatccc
    27661 ggagcaggag ccgcaggtcg gacacgcgga acgttcaacc tgatcgctct gggagtcaga
    27721 aactttcggg tctgcgccct ggatcatcgc atcaaccaga tcgagtttga ttatctgatc
    27781 ggacagtttg gttttcccgg cctccatcgg gccgccggaa acaaagatca ccggaatatt
    27841 caggcgcagg gaagccatca gcatccccgg ggtgattttg tcgcagttag agatgcagac
    27901 catggcgtcg gcgcagtggg cgttgaccat atactcaacg gaatcagcga tcagttcgcg
    27961 agatggaagt gaataaagca tccccccgtg gcccatggca atcccatcat ccaccgcaat
    28021 ggtgttgaac tctttggcaa cgccgccagc cgcttcaatt tgttcggcga ccagtttacc
    28081 gagatcgcgc agatggacgt gacccggtac aaattgggtg aacgagttca caaccgcgat
    28141 aatcggcttg ccaaaatcgg cgtcggtcat tccggtggcg cgccacagcg cacgagcacc
    28201 cgccatatta cgaccatgag tggtggtggc ggaacggtac ttaggcatac tttatttact
    28261 cccagtgtct gtctcgtaaa tgggacggtg cgtgccgtcc cattttttgt atttattgat
    28321 taacttgatc taaccagccc catttatctt cggtttcgcc agtgaagagg ccgaagaagg
    28381 cttgctgaat gcgtttggta accgggccac aacggccttc gccaatctga ataccgtcta
    28441 cgctgcgcac tggcgtgatt tctgccgccg taccggacat aaagacttca tccgccaagt
    28501 acagggattc gcgcgacagc acctgctcac gcacttcaat tcccagctct ttcgccagtt
    28561 tgatgatggc atcacgggta atacccggca gcgcggagga ggtgaacggt ggggtgaaca
    28621 gcacaccatc tttcacttca aacaggtttt cgcctgcgcc ttcagagata taaccgttca
    28681 catccagcgc gataccttcc tgataaccgt ggcggcgcgc ttcgctaccc accagcaggg
    28741 aagagagata gttaccaccg gcttttgccg cagttgggat ggtgtttggt gctgcgcggt
    28801 tccaggagga aaccatcgca tcgatcccct gctccagcgc ttctgcgccc agatacgctc
    28861 cccacgggaa agcggcgata atcacatcgg ttgagtatcc cgctggcggg tttactccca
    28921 tgccaacatc accgacgaag atcagcggac ggatataggc gctggtgaga ttgtttttgc
    28981 ggatcacgtc acgacaagct tccatcagct catcaatgct ctgtgaaacc gggaagcgat
    29041 agattttggc ggagtcatgc agacgctgca tatgctcacg atggcggaat acaaccggtc
    29101 ctttgtgtga gtcgtagcaa cggatgcctt caaaaaccga ggtgccatag tgcagcgcgt
    29161 gcgacatcac atgcaccttc gcgtcttccc agcgaaccat ctccccattg aaccaaatgt
    29221 aatcagcttt cttcgtggtc atttttatat tccttttgcg ctcaggcgcg gatttgttgt
    29281 gatgtggttg tgctctggca gatggcaacg tgtgcgacgt ccaccagttt atttaactga
    29341 ctaaacagta agtcgaccga ccgtgggctg gcaacggtca attcgatatt tatattttgt
    29401 gcatcgctgg cggcagccat attcattgag cagacgtgga aaccacgatg acgcaccacg
    29461 cgtaaaacac gttctaaggt ttccggattg aagcgagccg atacattgac ctgatgttgc
    29521 atcatgataa tttctccaac atttctgaat tactggcacc tggcggcacc agcggccaga
    29581 cgttctcaag ttcgtcgatt gagacatgaa gcaggtatgg cccatcactg ttcagcatgg
    29641 tgtcgagtgc cgcttcaacc tggtctttac gggtgatgtg ttggccaggg atgccgaagg
    29701 cgctggctaa catgaggaaa tcggggttat cggtaagggt ggtttcgctg tatcgttcct
    29761 gaaaaaacag ttgctgccat tgtcgaacca tccctaaccg ttggttatcg agtaagacga
    29821 ttttcaacgg taactgcttg cgttttacgg tgcccagctc ttgcacattc atcatgaaag
    29881 agccgtcacc ggagatacag acaacggtat cgttcggtcg cgcgacttgt gcgccaactg
    29941 ccgccggtaa accaaaaccc atggtaccta agccgctgga ggtgatgaaa ttttccgggc
    30001 gagtgtgggc gatgtgctgc gcagcccaca tctggtgctg ccccacatct gtggtcacga
    30061 cgcaatccgc aggtttacga tccgacagtt gttttaacaa caacggagcg tagatagcgt
    30121 caccgggatg gtcgtaacgc caggaatgtt catcacgcag ctgcgcgcag tgttgctgcc
    30181 agtcattgat atttaacggc tgctgtaatg ctggtaacag agcatttaaa tcaccttgta
    30241 atgccacatg tgcctgacgc agcttgttca tttctgccgg gtcgatatcc atatggataa
    30301 cactggcgtg tggtgcgaag gtgttcagtt tgccggtcac ccggtcatca aaacgtgcac
    30361 ccacggcgat cagcaggtca cactcctgca ccgcgaagtt tgccgctttg gttccgtgca
    30421 tccccagcat gcccagatag tacggataat ctgcttctac tgcgcccagc cctttcagcg
    30481 tacaggtggc aggcattttt gtggcagcga gaaattcacg caaagccgga actgcctgcg
    30541 ccatccccac gccaccgcca acgtacagca tcggtttttg cgcttttgcc agcatctggc
    30601 gcgcttgctc gacttcggca tgtgggaaag tcacttcgtt ttcaacggtg gtgaaccacg
    30661 gttccagatc gccgctggct aactggatat cttttgggat atcgaccaga accggaccag
    30721 gacgacctga gctggcaacg tcgaatgctt cagccatgat gcgcggcaac tcttccagag
    30781 actgcaccag aaagctgtgc ttggtacagg ctaacgacaa tcccaggaca tccacttcct
    30841 gaaatgcgtc ggtgccgata aacggtgcgg acacctgacc agtgatggca acgacaggga
    30901 tggaatctaa cagtgcgtcc gcaagcccgg ttatcaggtt ggttgcgccc ggaccagacg
    30961 tggcgataca tacgccagtt ttaccggtag cacgggcata accgatagcc gccattgccg
    31021 caccctgctc atgtcggcac agcaagtgct ccacgccgcc gtcatacaat gcatcgtaaa
    31081 ccggcataat tgcgccaccc ggataaccga aaacggtgtt cacaccctgt gcccgcaacg
    31141 catgtaccac ccactgtgcg ccattcatag ttagttcccc gtcctgaatc ttgagaaaca
    31201 gaattttgtg ctgttattca ttgtctgctc ctcggttatg tttttaaggt caaaaaaacc
    31261 cccggacctt tcggtgcggg ggtcttagtt cgttaaggct tgatctctaa gcctttcctc
    31321 gtccaagtgc agccccgcac ggtgggataa taatcaccac cacgctaatc acgaccaggc
    31381 taatcactcg tagaagggct gtcattttgt cttttcttgc atcttgttcg aaggaatgcc
    31441 taaagagtta ccatagattt tgcgaatagc acaagatatt tttgggccta tttttatgat
    31501 ggctttaata atttcaatag aaattgttgt tttttaaaaa gaaaatagtg agtgaaaaaa
    31561 ttcaatttca gtataaatgt tgttcccttc ctcgtagttc atcctttgtc cgctgcattt
    31621 ttgcgagcat cattccaccg ccagaacaac tcaacttcac ttgcacaaat aaacaggaat
    31681 cagccactaa aattcacgaa ttagcgggct acagcagcga tatcttatgc acataatggt
    31741 gactcaagga gggtttatgt cactgtcaat tgttcatacc cgcgcagccc tggaagtaaa
    31801 tgcgccccca atcactgttg aggtacatat cagtaaaggt ctacccggct taacgatggt
    31861 gggcttaaca gaaacaacgg tgaaagaagc tcgcgatcgc gtgcgcagcg ccattatcaa
    31921 tagcggatat gaatatccgg cgaaaaaaat caccatcaac cttgctccag ccgatctgcc
    31981 aaaagaaggg ggacgatatg atttacctat cgccattgcg ttgctggcgg cctcagaaca
    32041 gcttacagcc aataagttag atgaatatga attagtcgga gaactagcgc ttacaggcgc
    32101 tctgcgtggc gttcccggtg caatctccag tgcaactgaa gctattaagt cgggcagaaa
    32161 aattatcgtc gcgaaagata acgaagatga agtggggcta attaacggtg aaggatgcct
    32221 gatagccgat catctgcagg ctgtctgtgc gtttctggaa ggtaagaaca ctctcgaacg
    32281 cccgaaacca actgatgcag tatcccgggc gctacaacat gatctcagtg atgttgtcgg
    32341 tcaggagcaa ggaaaacgag gactggaaat taccgccgct ggtgggcaca accttttact
    32401 gattgggccg ccgggaacag gtaaaacaat gctcgccagc cgtattaatg gcctgttgcc
    32461 agatttaagc aatgaagagg cactggagag cgctgcgata ttaagtctgg taaatgctga
    32521 atcagtacaa aaacaatggc ggcagcgccc gttccgctca cctcatcaca gtgcgtcgtt
    32581 aactgcgatg gtgggcggtg gtgcaattcc agggccaggt gaaatttcgt tggcgcataa
    32641 cggcgtgctt tttcttgatg agctacctga atttgaacgg cgtacactgg atgccttgcg
    32701 agagccgatt gaatccgggc agatccatct ttcacgcaca cgagcaaaaa taacctatcc
    32761 agcccgtttc cagcttgttg cggcgatgaa tcccagccct accggacatt atcagggaaa
    32821 ccataaccgc tgcacgccag aacagacatt acgttatctc aaccggctct cggggccctt
    32881 tctcgaccgc ttcgatctct cactggagat cccattacca ccccccggca ttttgagtaa
    32941 aacggtagtg ccgggagaaa gcagcgccac cgttaaacaa cgcgtaatgg ccgccagaga
    33001 gcgccaattt aagcggcaga ataaactgaa tgcctggctg gatagtccgg aaatacgcca
    33061 attctgcaaa cttgagagcg aagatgcgat gtggttggaa gaaacgctga tccatctggg
    33121 gttatcgatt cgtgcctggc agcggttact gaaagttgca cgaaccattg ctgatattga
    33181 tcagtctgac attatcacac gtcagcattt gcaggaggca gttagctatc gtgcgattga
    33241 ccgtttgctc atccatctgc aaaaactact gacataaaaa aagggcattt cgcccttttt
    33301 attaatcgtc agaatcggtg tagtcttcag caccttcaac ctgcggttta ccgccggaaa
    33361 gggtgtgaaa acgttttgga cgcttaatac gcgtcatata cttggaccac acgcgttctg
    33421 cttctgtcac tggctcacgt tcgccacggc atactgctac gaagagtttc tcttcctcgg
    33481 taaccggctc gcgtttgcca agatccaact cattgaaggc ataaccatga cgctcaagca
    33541 gttgtgcctc tttgatggtg aaatcaccat gacgagagaa tccacgtgga taatgtttat
    33601 tgtcgaaata tcgattagtc gtcgtaaagc tttccgccat cctgcacgct cctaattctt
    33661 tgaccgagct agttatggcg cggagtatta gttacgcttg acagagtgta aaacaaaaca
    33721 tttaaatcat aacgacaaat aattttgcgg agagcactgt ggatacggaa ttgttaaaaa
    33781 ctttcctgga agttagccga acgcgtcact ttggtcgagc ggctgaatcg ctctatctga
    33841 cccagtcagc agtgagcttt cgaatcagac aactggaaaa tcaactgggt gtgaaccttt
    33901 tcacccgcca cagaaacaat atccgtttaa ccgctgccgg tgaaaaacta ctgccttatg
    33961 cagaaacgct catgagcacc tggcaggccg cccgcaagga ggtggcgcat acctcacgac
    34021 ataacgagtt ttctatcggt gccagcgcct cgttgtggga atgtatgctt aatcagtggt
    34081 tgggacgctt gtatcaaaat caggatgccc atacaggctt acagttcgaa gcgcgaattg
    34141 cccaacggca gtctctggta aaacagctgc atgaacgcca gcttgatctt cttattacca
    34201 ctgaagcgcc caaaatggac gaatttagta gtcagttgct ggggtatttc actttagcgc
    34261 tttataccag tgccccttct aaactaaagg gagatcttaa ctatctgcga ctggagtggg
    34321 ggccagattt tcaacagcat gaggcaggtt tgatcggtgc tgacgaagtt cccattctga
    34381 caaccagttc tgctgaactg gcacagcaac agattgcgat gcttaatggt tgcacctggc
    34441 tacccgtcag ctgggcgcgt aaaaaaggcg gcctgcatac cgttgtcgat agcacaacac
    34501 tttcacggcc gctttatgcc atatggctgc aaaatagcga taaaaatgcg ttgattcgcg
    34561 atcttttgaa aattaacgtg ctggatgaag tgtattaatc tgaatggctg gcaaggatgc
    34621 cggtagaagg atttacttcg gagaggatta tttcagataa aaaaaatcct tagctttcgc
    34681 taaggatgat ttctggcagg ggcggagaga ctcgaactcc caacacccgg ttttggagac
    34741 cggtgctcta ccaattgaac tacgccccta attag
//
LOCUS       DAJVPP010000034        34656 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVPP010000034 DAJVPP010000000
VERSION     DAJVPP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.034"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1966
                     /locus_tag="OQM34_001645"
     CDS             <1..1966
                     /locus_tag="OQM34_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCS1452136.1"
                     /translation="QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSR
                     NSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKT
                     GGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFEL
                     GNNWTADLGRVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTT
                     DSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQ
                     TTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTM
                     DSNNNSHSTNVPTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSA
                     VNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLG
                     AVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQ
                     GNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNS
                     AMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYS
                     QLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            1963..3051
                     /locus_tag="OQM34_001646"
     CDS             1963..3051
                     /locus_tag="OQM34_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS1452137.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3139..4437)
                     /gene="exuT"
                     /locus_tag="OQM34_001647"
     CDS             complement(3139..4437)
                     /gene="exuT"
                     /locus_tag="OQM34_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCS1452138.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4920..6332
                     /gene="uxaC"
                     /locus_tag="OQM34_001648"
     CDS             4920..6332
                     /gene="uxaC"
                     /locus_tag="OQM34_001648"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS1452139.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6347..7834
                     /gene="uxaA"
                     /locus_tag="OQM34_001649"
     CDS             6347..7834
                     /gene="uxaA"
                     /locus_tag="OQM34_001649"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCS1452140.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7917..8468
                     /locus_tag="OQM34_001650"
     CDS             7917..8468
                     /locus_tag="OQM34_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1452141.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8473..9717)
                     /gene="sstT"
                     /locus_tag="OQM34_001651"
     CDS             complement(8473..9717)
                     /gene="sstT"
                     /locus_tag="OQM34_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1452142.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10313..11278)
                     /gene="alx"
                     /locus_tag="OQM34_001652"
     CDS             complement(10313..11278)
                     /gene="alx"
                     /locus_tag="OQM34_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1452143.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11549..12547)
                     /locus_tag="OQM34_001653"
     CDS             complement(11549..12547)
                     /locus_tag="OQM34_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1452144.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSHSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12626..13318)
                     /locus_tag="OQM34_001654"
     CDS             complement(12626..13318)
                     /locus_tag="OQM34_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1452145.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13395..13898)
                     /locus_tag="OQM34_001655"
     CDS             complement(13395..13898)
                     /locus_tag="OQM34_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1452146.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            13983..15119
                     /gene="rlmG"
                     /locus_tag="OQM34_001656"
     CDS             13983..15119
                     /gene="rlmG"
                     /locus_tag="OQM34_001656"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1452147.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15404..15718
                     /gene="higB"
                     /locus_tag="OQM34_001657"
     CDS             15404..15718
                     /gene="higB"
                     /locus_tag="OQM34_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1452148.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15715..16131
                     /gene="higA"
                     /locus_tag="OQM34_001658"
     CDS             15715..16131
                     /gene="higA"
                     /locus_tag="OQM34_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1452149.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16176..18194)
                     /gene="fadH"
                     /locus_tag="OQM34_001659"
     CDS             complement(16176..18194)
                     /gene="fadH"
                     /locus_tag="OQM34_001659"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1452150.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGSAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18420..20771)
                     /gene="ygjK"
                     /locus_tag="OQM34_001660"
     CDS             complement(18420..20771)
                     /gene="ygjK"
                     /locus_tag="OQM34_001660"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCS1452151.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20788..21207)
                     /locus_tag="OQM34_001661"
     CDS             complement(20788..21207)
                     /locus_tag="OQM34_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452152.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21185..21538)
                     /locus_tag="OQM34_001662"
     CDS             complement(21185..21538)
                     /locus_tag="OQM34_001662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452153.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21539..21859)
                     /locus_tag="OQM34_001663"
     CDS             complement(21539..21859)
                     /locus_tag="OQM34_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452154.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(21993..23426)
                     /locus_tag="OQM34_001664"
     CDS             complement(21993..23426)
                     /locus_tag="OQM34_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1452155.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGHWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23489..23938)
                     /locus_tag="OQM34_001665"
     CDS             complement(23489..23938)
                     /locus_tag="OQM34_001665"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1452156.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(23935..27021)
                     /gene="ebgA"
                     /locus_tag="OQM34_001666"
     CDS             complement(23935..27021)
                     /gene="ebgA"
                     /locus_tag="OQM34_001666"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCS1452157.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRMMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27204..28187)
                     /gene="ebgR"
                     /locus_tag="OQM34_001667"
     CDS             complement(27204..28187)
                     /gene="ebgR"
                     /locus_tag="OQM34_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1452158.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28405..28736
                     /locus_tag="OQM34_001668"
                     /pseudo
     CDS             28405..28736
                     /locus_tag="OQM34_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28778..30157)
                     /gene="ygjG"
                     /locus_tag="OQM34_001669"
     CDS             complement(28778..30157)
                     /gene="ygjG"
                     /locus_tag="OQM34_001669"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1452159.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30575..32095
                     /gene="aer"
                     /locus_tag="OQM34_001670"
                     /pseudo
     CDS             30575..32095
                     /gene="aer"
                     /locus_tag="OQM34_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32249..32872)
                     /gene="nfeR"
                     /locus_tag="OQM34_001671"
                     /gene_synonym="yqjI"
     CDS             complement(32249..32872)
                     /gene="nfeR"
                     /locus_tag="OQM34_001671"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1452160.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33149..33894
                     /gene="nfeF"
                     /locus_tag="OQM34_001672"
                     /pseudo
     CDS             33149..33894
                     /gene="nfeF"
                     /locus_tag="OQM34_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(33948..34023)
                     /locus_tag="OQM34_001673"
     tRNA            complement(33948..34023)
                     /locus_tag="OQM34_001673"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33988..33990),aa:Ile,seq:cat)
     gene            34148..34654
                     /gene="mug"
                     /locus_tag="OQM34_001674"
                     /pseudo
     CDS             34148..34654
                     /gene="mug"
                     /locus_tag="OQM34_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8449 a         8920 c         9119 g         8168 t
ORIGIN      
        1 acaaggcggg aactttacct ttaactttct gccactcggt gcaatcgatg ggatgcgtat
       61 cgggtcgaca ctgagctatt taaaccaggc gcaaagccag caaggaaccc cagtaatggt
      121 tctgctttcg cgcaattctc gtgttgacgc ttatcgtaat gagcaacttt tgggatcgtt
      181 ttatctcaat agtggttcgc aatttattga taccagttcc tttccgccag gcagctacag
      241 cgtggcatta aaagtctacg aaaataacca actcacccgc accgagcttg tgccgtttac
      301 caaaaccggc ggtctgactg acggaaatgc gcaatggttc ttacaggcag gtaaaactac
      361 atcacaggtt tctgatgatg aaagttcagc ttatcagcta ggagtacgcc tgccattaca
      421 tccgcaatat gagctctacg cagggctggc gaatgccgat gatgtgagtg ctttcgagtt
      481 aggtaataac tggacggcag atttaggcag ggtggggaat cttgcaatca gcgccagcgt
      541 gttccgtaac gatgacggcg gcaaaggtga tatgcaacag gccaactgga gtaatccggg
      601 atggccgacg ttgggctttt atcggaccaa ctctgacggc gatgcttgta caaccgacag
      661 cagagagagc tataacgcct taagctgtta tgaaagtatt tccgcgacag tttcactgaa
      721 ttttgtcggc tggaatatga tgctgggtta tacctgcaca caaaataaca ctgatgatag
      781 tttgcgttgg gataaacagc agagctttga aaataactat cttcgccaga caacagcgca
      841 aagtatctct gaaactgtac aacttagcgc ttcccgcgct attgtgatgc gtgactggat
      901 tttgagtacg tcagtcggtg ttttccatcg taatgacaac ggtggcgata acgacgacaa
      961 cggcttgtac ttatcgtttt cgttatctga cacgccaacg atggatagca ataacaacag
     1021 ccattcaacc aatgtgccta cggattatcg ttatagcgaa caggatggcg atcaaacgtc
     1081 atggcagtta tcgcatacct tttataacga ttcattcagc cataaagaac ttggcgtaac
     1141 cgtcggaggc ctgaacaccg ataccataaa cagcgcggtt aacgggcgtt gggatgggca
     1201 atacggaaat gtctacgcta ccgtatctga tagttatgac cgtaagaatc atgatcatct
     1261 ttcggccttt acgggcactt atagctctac gctggctgtc agtcgctatg gcgttaattt
     1321 gggagccagt ggtacagacg atttgctggg tgcggtactg gtggatgtga aaggcttctc
     1381 tgaacaggat gaagagagtc aggatctgca actcgaagcg cgggtggcag gcagccgaac
     1441 tttgcagctt ggtcaaagtg acagtgtgtt gttcccttat cctggatttc agtctggttt
     1501 tgttgaggtt aacgacagta gccagggcaa tcagcagggg acaacaaaca tcattaacgg
     1561 tgcagggaat cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg aagtgtctgc
     1621 cagctttaac tacaactata tcggtcgttt attactgccg gcagcggtga aaaaattccc
     1681 gattgtgggg ctgaacagcg ccatgttact ggtagctgaa gatggcggat ttacacttga
     1741 aattaacggc agcgaaaaag agctctatct gctttccggg cagcaattcc ttaagtgtcc
     1801 actgagtgtt gtaaagaaac gcgccagcat tcgttacagc ggagatgtta cttgtagtgt
     1861 ggtgacttat tcacaattac cggagtcgat tcaggttcag gcacagttga aacagcctaa
     1921 attacgtgga aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt
     1981 gcggcgatat tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc
     2041 agtgaaatta cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa
     2101 gcgtcggcag gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa
     2161 acctgtacag ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt
     2221 gggatgcgtt ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa
     2281 gcgccgctag gatgcaaggg gggcaaaggg cttcagacga atcttaagga ttctaataga
     2341 tcttcatgta cagaagatgg tcaacattat tatatatacg atacaaagtt tcttacgctc
     2401 taccttgagc agacagagat gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt
     2461 aaattacatt cgaacagccc ggcccaggac tatttcgcaa atattaccct gaatacgctc
     2521 gaccccaacc atattgacgt gttcttcccg gagttcgccc acgccacgcc aagggtgcag
     2581 ttagacttgc atccaacagg aagcgttaac ggcagcaact acgcgcaaga tctgaccatg
     2641 ttggacatgt gcctgtacga tggttttaac ggtaatgcca tcagttatga aatcatgctc
     2701 aaagatgaag ggcgaccagc tgcagggcgc agagacggtt acttctctat ctatcgtcag
     2761 ggagggacca ccaccgacga gggagaacgc attgattacc gggtcaaaat gtacaacccg
     2821 gaaaccggtg ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa cagcattaac
     2881 ctgaaacgtg tgcgtccggt ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg
     2941 acacccttga ctctggcagt cgataaattc agcgtgatgg ataaacaggc cgggtactac
     3001 atgggcaaat tgtcagtaat ctttacgcct tccttgccaa ccatcaatta accctgcaaa
     3061 ccggacaaac gcttttttct atataaacga cgaagccgcc tgataaggcg gcttcgtcgt
     3121 tataacttac gttaaacgtt aatgttgcgg tgcgggatcg ttatgtgttt cctgcgccac
     3181 ctcaatcgcc ggtttgttct gcaatacggt ccagataacc agcgcaccca gcaggtcaaa
     3241 tactgccaga actgcgaaca gcgggctgaa gccgatggtg tcagccagtg caccgacaac
     3301 cagcgcaaac agcgtacttg ccagccatgc ggacatcccg gttaaaccgt tcgccgtcgc
     3361 cacttcgtta cgaccgaaca cgtcagaaga gagcgtaatc agcgcgccag acagtgcctg
     3421 gtgggcaaaa ccaccgatac acagcagcat aattgcgaca tacgggttgg tgaacagacc
     3481 gatcataccc gggccaatca tcagcacagc acccagcgtt acgaccatct tacgggaaac
     3541 gatcaggttc acaccaaacc aacgctggaa cagcggcggc aggtaaccac cgaggataca
     3601 accgaggtca gcaaacagca tcggcatcca ggcgaacatc gcgatctctt tcaggttaaa
     3661 gccgtaaact ttaaacatga acagcgggat ccacgcgtta aaagtacccc aggccggttc
     3721 tgcaaggaaa cgcggcagtg cgatacccca gaactgacgg ttacgcagga tctgaccaac
     3781 ggacattttc ttcgccgtgc tcacctggtg ctgggcttcc tgaccattaa taatatagtc
     3841 gcgttcttca tcggtcagat gcttctggtc gcgcggatgt ttatagaaaa tcagccatgc
     3901 catcgcccag ataaagctca atgcaccgga gatgataaat gccatctgcc agctgtgcat
     3961 cacgattgcc cataccacca gcggcggcgc aatcatcgca ccaatcgaag aacctacgtt
     4021 aaagtagcct actgcgatgg aacgctcttt cgccgggaac cattcggagc tggctttcag
     4081 acccgccggg atcatcgctg cttccgcggc accgaccgca ccacgagcaa cagctaggcc
     4141 accccagcta cctgccagcg cggttgcacc acagaacacg gcccacagca cagcaaacat
     4201 tgcataaccg attttcgtac ccagcacatc cagtacataa cctgctaccg gttgcatgac
     4261 cgtataagca gcagaatagg ctgcgatgat ataggaatac tgttgggtgg agatgtttaa
     4321 ctcttccatc agagttggcg cagctgccgc cacagtgtta cgcgtcaggt aaccaagcac
     4381 ggtgccgagc gtcaccagtg cgatcatata ccaacgtaac cctttaattt tacgcatgta
     4441 aaacctcatc gttttgttat atccgcagga agcgcggcca tcatcccgcc agaggctgaa
     4501 gatgttagtt acgaccgggt aaccgaaaaa aaaccggcac gccccgaacg ttgccatgaa
     4561 agtttcgtgc taattcggct tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa
     4621 tcggcaattt gcattccgtc gacttatgat ttgcgacggc agaaagataa cttgtcatac
     4681 aaccttaaaa ggtgagagcc atcacaaatg tgcgaatatt tgtagggaca ttacctgacg
     4741 acagcgaggc cagtactggc gcggcctgca gcgagattta ccactttgag agtaattttt
     4801 ttaactacgt ttattgatct aactcacgaa aatatcttcg gactctggaa attggtgtga
     4861 taactttgtc agcatcgcac cataagcaag ctagctcact cgttgagagg aagacgaaaa
     4921 tgactccgtt tatgactgaa gatttcctgt tagataccga atttgcccgc cgtctgtatc
     4981 acgactacgc aaaagaccag ccgattttcg attaccattg ccatttgccg ccgcagcaga
     5041 ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg gttgaagggc gatcactaca
     5101 aatggcgcgc tatgcgtacc aacggtgtgg ctgagcgtct gtgtactggt gatgcgtctg
     5161 accgtgaaaa atttgacgcc tgggcggcga ctgttccgca cactatcggc aacccgttat
     5221 accactggac gcacctcgaa ctgcgtcgtc cgttcggtat cactggcaaa ttgctttctc
     5281 cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact gctggcgcag gataacttct
     5341 ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt cggcaccacc gatgacccga
     5401 tcgattctct ggagcatcac gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc
     5461 tgccgagctg gcgtccggac aaagccttca atatcgaaca ggcgaccttt aacgactaca
     5521 tggcgaagct gggcgaagtt tccgataccg acattcgccg ctttgctgac ctgcaaactg
     5581 ccttgaccaa acgtctggat cacttcgccg ctcacggctg taaagtgtct gaccatgcgc
     5641 tggacgtggt gatgtttgct gaagcgaacg aagcggaact ggacagcatt ctggcgcgcc
     5701 gtctggctgg cgaaaccctg agcgagcacg aagtggcaca gttcaaaact gcggtactgg
     5761 tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca gcagtaccac attggcgcgc
     5821 tgcgtaataa caacctgcgt cagttcaaac tgctggggcc ggatgtaggc tttgactcca
     5881 tcaacgaccg tccgatggcg gaagagttgt ctaagctgct gagcaagcag aacgaagaaa
     5941 acctgctgcc gaaaaccatt ctctactgcc tgaacccgcg cgataacgaa gtgctgggca
     6001 ccatgatcgg taacttccag ggcgaaggta tgccgggcaa aatgcagttc ggttccggct
     6061 ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat gacccaactg gcgcagctcg
     6121 gtctgctgag ccgctttgtc ggtatgctga ctgacagccg tagcttcctg tcatacaccc
     6181 gtcacgaata cttccgccgc attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg
     6241 aagcaccggc ggacatcaac ctgctgggcg agatggtgaa aaatatttgc tttaacaatg
     6301 cgcgtgacta cttcgccatt gaactgaact aaggtctggg gttgatatgc aatacatcaa
     6361 gatccatgcg ctggataacg tcgcggtcgc tttagcagat ttggctgaag gcacagaagt
     6421 cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt gctcgtggac ataaatttgc
     6481 gttaacggat atcgcaaaag gggccaacgt catcaaatat ggcctgccga ttggttatgc
     6541 attggcggat attgcggcgg gggaacacgt ccacgcccac aatacgcgca cgaatctgag
     6601 cgatctggat cagtatcgct atcaacctga ttttcaggat ctgcctgcgc aagcggcaga
     6661 tcctgaagtg cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta acgagctgtg
     6721 gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg caaatccaga accgtttcct
     6781 gaaagagacc aacaacgccg aaggtaccga cggcgtgttc ctcttcagcc acacctatgg
     6841 ctgctcacaa ctgggcgacg atcacatcaa cacccgcacc atgctgcaaa acatggtgcg
     6901 ccacccgaac gcgggcgcag tgctggtgat tggtctgggc tgtgaaaaca accaggttgc
     6961 cgcattccgt gaaacgttgg gcgatatcga tcctgaacgc gttcatttca tgatctgcca
     7021 acagcaggat gatgagatcg aagccgggat cgagcatttg catcagctgt ataacgtgat
     7081 gcgcaacgat aaacgcgagc caggcaaact cagcgaactg aagtttggtc tggagtgcgg
     7141 tggttctgac ggtctttctg gtattactgc taacccgatg ctggggcgtt tctctgacta
     7201 cgtgattgct aacggcggta ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga
     7261 gcagttgctg atggaccatt gccgcgacga agcaacgttt gaaaaactgg tcaccatggt
     7321 caacgacttc aaacagtact ttattgccca tgatcagccg atctacgaaa acccatcacc
     7381 ggggaacaaa gcgggcggta tcaccacgct ggaagacaaa tcacttggct gtacccagaa
     7441 agcgggttcc agtgtcgtgg ttgacgtgct gcgttacggc gagcgtctga aaacgccagg
     7501 gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc tggcgggtgc
     7561 gggctgccat atggtgctgt tcagtactgg tcgcggcacg ccatacggtg gatttgtgcc
     7621 gacggtgaaa atcgccacca acagtgaact ggcggcgaag aaaaaacact ggatcgactt
     7681 tgacgcgggt cagctgatcc acggtaaagc gatgccgcag ttgctggaag aatttatcga
     7741 caccatcgtt gagtttgcca acggtaagca aacctgcaac gagcgtaacg acttccgcga
     7801 actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt taataaaaaa
     7861 cggcgtttca taatgtgaag cgccgtttgc ttttccatac ctgctaagga tttatcatga
     7921 ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt tctgattggt atcacaacat
     7981 ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc ggtctatagc gccgttattg
     8041 gcgtacattt ttttcttctg ggcacctatc ccgctggtgc cagtgccatc cttaatgcca
     8101 ttcgtacatt gattacctta cgcacgcgca gcttatgggt aatggcgatt tttattgtgc
     8161 tgactggcgg aattggcctc gcgaagttcc atcatcctgt cgaactattg ccggttatcg
     8221 gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg gctcaccatg cgctgcgtga
     8281 tgtggttttc aacgtgttgc tgggtgattc acaacttctg ggcggggtcg ataggcggca
     8341 cgatgattga gggtagtttt ctgcttatga atggcctgaa tatcattcgt ttctggcgga
     8401 tgcagaaaag gggaattgat ccgtttaaag tagagaaaac ccctcccgcc atagacgaaa
     8461 ggggttaaac aattaattac gcagggcgct atttgccaga cgatcgtctt ccgcctggca
     8521 agctgccgca gtgaacagca cgtcagttga agagttcagc gcggtttcgc aagagtcctg
     8581 caatacgccg ataataaagc cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc
     8641 gaacatatta caggccagtg ggatcagcag caaagaaccc cccgccacgc cggatgcgcc
     8701 acaggcacac agagaagcca ctacgctcaa cagcagcgct gtgggcagat cgaccggaat
     8761 acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat
     8821 attgatggtg gctcccagcg gaatagaaac tgaataggta tcgcgatcca gattcagctt
     8881 ttcacacagc gccatattca ccggaatgtt cgctgcagag ctgcgggtga agaaggcata
     8941 cacaccgctt tcgcgcaggc acagcagcac cagcgggaac gggttacgac gaattttcca
     9001 ccacaccagc aatgggttaa ccaccagcgc caccagtaac atacagccaa ccagcacgac
     9061 cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg tagaagaaac
     9121 cagcccaaaa ataccgaacg gtgcgaagcg aatgaccagt ttcaccataa aggtaacggc
     9181 attcgacata tcattgacca ggtttttggt ggtctcgtta ccgtgacgca gtgcgaagcc
     9241 gaggccgatc gcccacacca gaatcccgat gtagttacct ttcagcaggg cgtcgatggg
     9301 gttggaaacc atgctcatta ccagtccgcg catcacttcg acaatgcctg acggcggcga
     9361 aatatcaccc gcgctactgg acaagtgcag ggtagaaggg aaggcaaagc tgaagactac
     9421 tgcggccaga gctgcagaga aggtgcccag cagatagagg aacaaaatag gacggatatt
     9481 ggttttctgc ccgtgctggt ggttagcaat agatgccatt accagcatca acaccaggat
     9541 gggggcaaca gctttcagtg cgccgacgaa caaagtacct aacagaccaa cagcttccgc
     9601 cgcgggtttt gagatccatg ccagaagaat ccccagaaca aggccgacca ggatttgttt
     9661 taccaggctg ccatgagcca gacgccggaa tagccccggt gaacgttgcg tagtcatttt
     9721 tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc atctttcagg
     9781 atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg gaagcgaaaa
     9841 atgctggatt ttaaagagag gtcatgtttt gcaacttaat ggttgcattt atttaacata
     9901 aacagaggga aggactggct actaaggctg attagataac atatgggcaa aactatttgg
     9961 aatatgagaa cggttgcgct cctttaacgt gttatgccat ataaagtcac tggcgcaggg
    10021 agcgcgcagg gggcggccaa tcgccgccgc cccctgcacc cccaggctct ggcgaacaaa
    10081 atcgccgctg cgcggttccc tcagcctatc ccttgcggct accgggtcgg gcgcaaggta
    10141 acgtccctgt aagatgcgcc ctcgccccgg cctccaggga acgcttcggc gattttgacg
    10201 ccaccaacac ccgtgcggtc tttttgtaaa gagctataag ccgattacat aattaatagc
    10261 tcacctttgt ctggaatgag ttatctctag gaatggctta atatacagcg aatcattctc
    10321 cccgctgctt atcatgccga taattcaccc aggcgttgat aataaacgtc atcaccagaa
    10381 tgccaaacac cacgcccagc gagacggcga ttggaatatg gtagaagtcg acaatcagca
    10441 tcttgatacc gataaacacc agaatcaccg ccaggccata tttgagcatc gagaaacgct
    10501 ctgctacgcc cgccagcagg aaatacatcg cacgcaggcc gaggattgca aacaggtttg
    10561 aggtcagcac aatgaacggg tcagtggtca cggcgaagat agccggaatg ctatccacgg
    10621 caaaaatcac gtcgctcaat tccaccagaa tcagtaccag catcagcggt gtggcgtaca
    10681 gcaatccatt cttacgcaca aagaaatgct cgttgtcgat ggtgtcggtc atgcgcaaat
    10741 gaccgcgtag ccagcgcacc agcggcttgt cgccaatgcc tgattcatct tcatgggcga
    10801 gcgccatctt cacgccggta aacagcagga aggcaccgaa gatatacagg atccagtcga
    10861 actgcgaaat cagccagctg cctgtgaaga tcatgatggt acgcagaaca atcgccccga
    10921 gcacgccata caccagcacg cgacgttgta atgccgccgg aacggagaaa tagctgaaca
    10981 acatcagcca gacaaagacg ttatcgaccg ccagcgattt ctcaatcaga taacccgtga
    11041 gaaaggccag ggcctgtgga tcggcaacgg cgcgaccttc ggtttgcacc agataccacc
    11101 agaaagcggc gttaaacagt aacgacagcg tcacccagac cagcgaccag gccgcagcct
    11161 gtttcatggt catggcatgt gccccacgac gcccctgcaa caacaggtcg atagccagca
    11221 taatggcgac aacaacagcg aatccgcccc atagcaacgg cgtgccgaca gtattcatag
    11281 aagttcctta cacataaaaa aacggccaac gtcggaagac gtcagccgct gctttttatg
    11341 catagacctc gccttccggc aaggtctcac ttacaacaaa aaaggaatac gtctgcgtat
    11401 tcctttcggg ttgcccggtg accggatgtg gtttttcaca catcgtaatg acgatcgacc
    11461 ggcaatgaag ttactcccct ttgcaggtaa caaagtatga cagaccattc agtcagtcaa
    11521 tggtctgtta catacctttt acactgtttt actttggcaa gtatagtttt acgctatctg
    11581 ccggaaaaat caccccagtt tggcggcgga tctcggtcag cagtttggct gtgatgcgac
    11641 tgaccgccag ccctggatga tccaccagat gctcatccac cagggtagcg aacagctctg
    11701 cttcgtagag catggtatta atatgctgcg gctgggtgag atcctgcatc tggctgccac
    11761 gcggcacaaa gcagactttc tggcattcag acagtttttc aatcaccagc gatcccgctt
    11821 cgccctgaat ctcgcttccc aggacagaat cactgacctt tgagtgctgc aaggtgacgc
    11881 tgaaatcacc gtaatccatc accaccacgc cgtgggcgtc aacgccgctt gccagcaaac
    11941 tcgccgttgc ctgcacgctt ttcggctcac caaataacgc caccgccgac gccaggcagt
    12001 aaaagccgat atccataatt gaaccgttag agaatgccgg attaaaggtg ttgggattct
    12061 caccgtccag gtaacgttga taccgcgagg aatactggca atagttgaaa aagactttac
    12121 gcagtttgcc tgctttcggc agcgcctggc gcaacaaatg aaagttcggc aggcaggcgg
    12181 ttttaaatgc ctcaaacagc accacctgat tttcgcgcgc acaggcaatg gcggcatcca
    12241 cttccgccag attcgacgcc agcggttttt cgcaaatcac atgaatttta tggctaagga
    12301 aaagttgtgt ctgggaaaag tgcagggaat tcgggctggc aatatacacc gcgtcaatgg
    12361 catcgctttc cgccatcgct tccagcgacg taaacagatg ctcgacagaa aaatcattgg
    12421 cgaagtgctg agcctgctca aggctgtggg aatatacggc ggttaacttg tatttaccgc
    12481 tctcatgggc ggcctcgacg aactggcgag tgatccagtt cgtaccaatc acagcgaaac
    12541 gtatcataac gcgtggatac tccataaagg gattctgtcg tcaatgtagc acgccttttt
    12601 catcttcgac gtgcgcagat acgcattatt ctttcgccgg acagccatgc tcactaaacg
    12661 gcccaatcat gaccgatggc ccgagatatt tcggctggcg atggaggatt tttgcgtcaa
    12721 tcaccgcgct gacacgagcg agtttctccc gcagttgagt ataaaaaccg tgcttcatat
    12781 tgaggtctgg cgcgttaaac ccgatagcgt cgatgccgta ctgttttgcc agccagatgg
    12841 cgcgttggtt atgaaattct tgcgagataa tggtgatatg gttttcccca aacacctttt
    12901 tggcacgcac taccgaatcc agcgttgaga atccggcata gtcgcaaaag atcactttcg
    12961 ccggtacgcc tttagcgatc aaagcctgct gcatacctga tgcttcatcg taatttttac
    13021 gtccgttatc gccactcacc agtagccatt tcacttttcc ggcgtggtac aacgctgctg
    13081 cggtatcaat acgtcgggta aagtagcgat tacccggcct cgcccccagc aataaaccca
    13141 cgttacgcgc cgggacggcg ttgacatcgc cccaggtcag ctgcttgctg gcgttgacca
    13201 taacgcgatc ggcgataaaa atggtcgctc ctgccacaag taacagtaag caggcaatct
    13261 ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg ggcaaatgcg cgcagcatgg
    13321 tcgttatcct taacgaaagg ggggaggtcg attttaggcg ctaaagaaag aaaagataac
    13381 gcctgacatc attttcagat tttgtcctgc ccaagcgaca actgcgttag ccacagccgg
    13441 gtgtcgaact caagctggtg gtactgcggc tccatatggc agcacaattg atagaacgct
    13501 ttgttatgct ctttctcctt caggtgcgcc agctcgtgca ccacgatcat gcgcaaaaac
    13561 ggttccggcg cgttgcgaaa cacggtagcg acgcggatct ctgcttttgc tttcagcttg
    13621 ccgccctgca cacgagaaac agcggtatgt aacccgagcg cgttttttag cacatggatc
    13681 ttattgtcat acatcacttt attgatcggc ggggcattac gcagaaactg atttttcaga
    13741 tcctgagtat attgccagag agctttatcg gtggcgtagt cgtgtgttcc cggatagcgt
    13801 tttgccagca catcgcccag acgctgttcg ttaatcagtg ttcgcacctg cgaaagcagc
    13861 tgctcgggat aaccctgaag ataagtaaga ttgctcataa ccgcccacga aaaagagaaa
    13921 aagggtatac tcacgcaccc ttttcagggg caacgtcgaa attttatcat tcaggagggc
    13981 cgatgagcca cttagacaac ggtttccgtt cactgacact acaacgtttt ccggcgacgg
    14041 atgacgttaa cccgctacag gcgtgggaag cggcagatga atatttgctg caacagttgg
    14101 acgacacaga aatccccggc ccggtgttga tcctgaatga tgcctttggt gcgttaagct
    14161 gcgcgctggc ggaacataag ccgtacagca ttggcgactc atacatcagt gaactggcga
    14221 cgcgcgagaa tttacgccta aacgggattg atgagtcgag cgtgaagttt ctcgacagca
    14281 ccgccgacta cccgcaacag ccgggtgtgg tgctgatcaa agtgccgaaa acactggcat
    14341 tgctggaaca gcaactgcgt gcgctgcgca aagtggtcac gtcggataca cgtattattg
    14401 ccggtgccaa agcccgtgac attcacactt ccacgctgga actgttcgaa aaagtgctcg
    14461 gcccgaccac caccacgctg gcatggaaaa aagcgcgcct gattaattgc acgttcaatg
    14521 aaccgccgct ggccgatgcg ccgcagaccg ttagctggaa gctggaaggt actgactgga
    14581 ctatccataa ccatgcgaat gtcttctccc gcaccgggct tgatatcggc gcgcgcttct
    14641 ttatgcaaca tctgccagag aatctcgaag gggagattgt cgatctcggt tgcggtaatg
    14701 gcgttattgg tctgacgctg cttgataaaa acccgcaggc gaaagtggtg tttgtcgatg
    14761 aatcgccgat ggcggttgct tccagccgtt tgaacgttga aaccaacatg ccagaggcgt
    14821 tggatcgcag cgagtttatg atcaacaacg cgctctccgg cgtggagcct ttccgcttta
    14881 atgctgtgct ctgcaaccct ccgtttcacc aacaacatgc gctgaccgat aacgtcgcct
    14941 gggagatgtt ccactacgcc cgccgctgcc tgaaaatcaa cggtgagctg tatatcgttg
    15001 ccaaccgcca tctggattac ttccataaac tgaagaaaat tttcggcaac tgcaccacta
    15061 ttgcgacgaa taataaattt gtggtgctga aagcggtgaa gctgggacgt cgtcggtaag
    15121 attgggagtt acctgccgga tgcggcgtaa acgccttatc cggcctacgt tcagcacgtt
    15181 acccccaggc cggataagac gcggcaagcg tcgcatccgg caatgtctgc tcagttctgg
    15241 aatcgcgctt ccataaaaca aaaaatccct gtcaccatcc tctacattct cttgtttagc
    15301 gtttttctac gtttattctt ccgtcacaca gataaattca tccgttgcca atctgtcaac
    15361 ggatgttatc atattgccaa tttgtcaacg gacgtgatga agaatgcacc tgataactca
    15421 aaaagcattg aaagatgctg cggaaaaata cccgcaacat aaaacggagt tggtggctct
    15481 ggggaacacg attgctaagg gatatttcaa aaaacctgag tcattaaaag cagtattccc
    15541 atctctggat aacttcaaat atctggataa gcattatgtt ttcaatgttg ggggcaatga
    15601 attacgtgtt gtagcaatgg tcttttttga atcgcaaaag tgctacatac gtgaagttat
    15661 gacgcataaa gaatacgatt tctttaccgc tgttcatcgt actaagggga aaaaatgatt
    15721 gcgattgccg acatcttgca agcaggagaa aagctaactg ctgtggcacc ttttctggcg
    15781 ggtattcaga acgaggaaca atacacccag gcgctggaac tggtagatca tctgctgctc
    15841 aacgatcctg aaaacccctt gctggatctg gtgtgtgcca aaataaccgc gtgggaagaa
    15901 tcagcgcccg aatttgcgga atttaatgcc atggctcaag ccatgcctgg cggtatagcc
    15961 gtgattcgta cccttatgga tcaatatggt ttaacccttt ccgatctgcc ggaaattggc
    16021 agtaaatcta tggtgtcacg cgttttgagc gggaagagga aattaacgct ggaacacgct
    16081 aaaaaattgg caacgcgatt cggcatttct cccgccttgt ttattgatta acgcgacgtg
    16141 ccggatgcgt tgcatccggc gcttcaaata acgatttaaa tctccagcgc cagccgtgtt
    16201 ccctgggcaa ttgcccgtcg tgcgtccagc tccatagcca catcgcagcc gccaattaaa
    16261 tgcacagttt tcccgctatc aatcagcggt tgcgccagcg cgcggtttgg ctcttgccct
    16321 gcgcagatca ccacattgtc cactgctaat acctgcgttt cgccgttgat caccacatgc
    16381 agcccgtcat cgtcaatctt ctgataactt acgcctggga tcattttcac accccgcgag
    16441 agcaggatgg tgcgatggat ccagccggtg gttttgccta acccctgtcc cggtttgctg
    16501 gctttgcgct ggagcatcac aatttgccgt gggctacgcg ggatctgcat tccctgcggg
    16561 cttaagccac cagcctgttg taggctgctg tcgatccccc actcattaca gaacctggcg
    16621 atattctggc tggtggattc gcccggctga cttaaataca tcgccgtatc aaaaccaatc
    16681 ccgccacaac cgatgatggc aaccttgttg cctaccggcg ctttgtcgcg cagtacatcg
    16741 agataactca ataccttcgg atgatcgatc ccgtcgatag gcggaacacg cggcacgatc
    16801 ccactggcga ggatcgtttc atcgaacgcc tgtaactgat ccgccgtcac ggtgtgattg
    16861 agtttcagcg tcacgcccgt cacttcgatc atccggcggt agtagcgcag cgtttcgtaa
    16921 aactcctctt tgccggggat ctgtttggcg atattaaact gcccgccgat ctcgctgtgc
    16981 gcatcaaaca atgttacctg atgcccacgc gccgccgcgt taatggcaaa cgccagccca
    17041 gcaggtccag cgccgaccac cgccagattt tttttctgca cggcgggaag gattggcatt
    17101 ttggtttcgt ggcaggcgcg aggattcacc aggcacgagg tgactttgcc aacgaagatc
    17161 tgatcgaggc agccctggtt gcagccaata caagtgttga tctcatcggc tcgccctgat
    17221 tgcgcttttg acagcagttc cgcatccgcg agaaacggtc gcgccatcga tactatatcg
    17281 gcatcgccgc gcgagagaat atcgtcggca acctgcggat cgttaatccg gttggtggtt
    17341 accagcggca gcgagacgtg gcctttcagt ttgcgcgtga cccagctaaa tgcgccgcgc
    17401 ggcacgggcg tggcgatggt cggaatccgc gcttcgtgcc agcctattcc ggtgttgata
    17461 atggtcgcgc ccgccgcttc aatggcctgc gccagttcta ccgtttcggc aaaagtgccg
    17521 ccgccttcga ccaggtcgag catcgacaac cggtaaataa taatgaagtc gttgccgacg
    17581 cgttcgcgca ccgtacgcac tacttctacg gcaaatcgca tccggttgcg gtaatcgccg
    17641 ccccactggt cactacgctg attggtgcgc agcgtcagaa attcgttgat caaataccct
    17701 tcagagccca tcacttcgac gccatcgtat ccggcctccc gcgccagttg cgcgcagcgg
    17761 gcgaaatcgt cgatcagttg caggatctct tcatgggtga gttcgtgagg aacgaaacgg
    17821 ttgatggggg cctgcaatgc ggacggtgcg accagatgcg gttggtagct gtagcgcccg
    17881 gtatgcaaaa tttgcagggc gattttgccg ccttcctgat gtacggcttc ggtaatggtg
    17941 cgatggtgtg ggatctggct ggcgtcgttg agcattgcgc taccttccat gccaacgcct
    18001 gttaaatctg gtgcaatgcc gccgctgaca atcagcgcca cgccgtgacg ggcgcgttcg
    18061 gcataaaacg cagccagccg ttcagcaccg tccgggtatt cctccagccc ggtgtgcatt
    18121 gaacccatca acacgcggtt ttttaacgtg gtaaaaccta aatccagcgg ggcgaacagc
    18181 gacgggtagc tcataaaatt gtccagtatg taaaataatt gttatgtagt cggatgagtt
    18241 ctaatttagc cgtcgatgga gaaaagggaa aaggggagtg cggggtttgt gatgggattc
    18301 aaaaagaata cgcccggtgg taacgccgga tgcgacgctt gccgtgtttt atccggcttg
    18361 ggggattgca catgtagggc ggataaggcg ttcacgccgc atccgccagc cgttgcaaat
    18421 tactgcttac ggaaaaaatc gttatacaac atatacaaat gagcggcact ccaggagaaa
    18481 tttggtgcgc cttgctgcgc gcctgtcagc gggttgtaat tctcctgaat tgggccatcg
    18541 gcggttaatc ctttggcgtg cctgaagaac gtatccgcca gcttcagggc atcatcacga
    18601 taaccgtaac gctccatccc tttcagacca aaccagaact gatccaccca tacgcgcccg
    18661 cgccagtaga tatcagcgcc aaaagccgga ttggttaacg ccgccgttcc cagcggaaca
    18721 aaggtgttga actctttagg atcgagcatc accttcacca ccgcgtcggc attggcctgc
    18781 gttgccgcac cgttaaacag cggcgaccag ccttccggcc ctttaccgcg ctcaacaatc
    18841 ggtttgcccg cgcagccgtt cgccagtggt ttatcttcaa tacgtacgtc atagtagaac
    18901 tgggtagtcg ggtcgaacat acaggtgttg atgtagtccg cgagctgctg tgccaactgg
    18961 cgatagcgtt tggcctcttc tggcttaccg aggatggttg ccatctccgc cagataatgg
    19021 ttatcgctgt acatatagct ggcctgatcc accgactcct gcaatagcga gtagcccagc
    19081 aacgttccgt cctgactgcg gttttcggcg aatttcaccg tccagtcgct acgtttgccg
    19141 ccattagcga catatttatc caactgttct ttgtcgataa aaccaaagac ggcggcatca
    19201 tcacggcccg attcccacga cgcagcgacc tgtgccggaa tttccagact gtcgtactgg
    19261 cctttctcca ccacgcgggc gtagttgttc agcccagact gcgtctcttc tttatcgcct
    19321 tttttcaccg taaacagcat ctcgccgctc tcggtgttgt gggctttgtc gcgggtcgcg
    19381 ccatattccg gcaccccgtt gccgttatga tcgcggttac gtaaccacca gtcgtgatag
    19441 gccaccagtt tcgggtacat ctctgccaac caggctttat cctgggtgac gttgtatact
    19501 tccatcaccg accaggcggc aaggctgggt ttggtattac gttcgttcca gttgccgcca
    19561 tcgccaccac gctcggggct aaggttccac gctatcaggt cggggacaaa gcccacatcc
    19621 tgcggacgca cgctgtcgcc aggctggatc tgccaggaga agaccgcgcg gatattctct
    19681 ttggcgatat ccggattgaa atgcgccatc gcaaacgcct gcttccaggt atcccacggc
    19741 caggtctgat tgccggagaa ccagcgtccg gtcaccgacg gcgtgacggt gttataattc
    19801 accgcaccgc ccggtgagcg ccagttaccg ttgagcgttt cgatcgcttt caccgcgacg
    19861 cgcgtctgtt ccggcgtcgc atccggattg gttaaccctt tcttcaggta ttcttcccag
    19921 cgttgctgcg aggcggtgag ataaaacgcc ggacgcgcca gaatatcgcg gatctgcatt
    19981 tgctctttgc taacttcctg agcggtcagc agatgggaat aggtggtgta gagcgtggtc
    20041 gaaccgttga tatgcgcctt actggtaaag cgattaccgt tgatttcagt ctgcaccggc
    20101 agggatttat gcacctgata ttccgattcg ccggaggtca gcagatccca ggtggcgcgc
    20161 actttgccaa aggtgacttt caggccatca cgggtggcgc tgattttgcg ctgatagtca
    20221 gggtattcgc cggcaatggt tttatcggaa agcggtttcc cttctttcgc ttccagtttt
    20281 tccagcagtt cgccatccca caccagatcc agcggtttat tgctgatgat tttagtttcc
    20341 agtagtgacg tgcgcggcgt ggcgaagcgc agagtcattt cgacctgcac atcttttgct
    20401 gtcagttttt gcaccagcgc gccgggaata ctgtatgcct ccagcgtgaa gttgactttc
    20461 ttgccgtctt gccagacagt caggcggtcg aaattgctgg ccataaagtt gatgtattct
    20521 tccgtcagca gcgcaacgcc cggaaagccg cccatggtgt tcgggccgtc tggcaacaga
    20581 tgaccatgcc aggctccgag atcgaaaaac ggattaaaac gctggtgatc gtcgtaatcg
    20641 taatccttca tgtactgcgg cgcaccagta cggttaatca cgtttttata attgtcggcg
    20701 ttagcggcat gagcggaaaa acttatcagc agagcgcagg ttactggcgt taaaatagtt
    20761 ttgattttca tgagattaca gtccttatta ccagtagaaa tattgcatca ggaagatttg
    20821 gtcgctggga tcgttagagg catttttcat gtagaagcgg ctatcaaaag ccgtggcgaa
    20881 acgtccgccg ccatattcgt accagatacc aaactgcatg aaagaggtgg tttcatcgtc
    20941 agcgtggtca gggcgatgtt tggcgtaggt gtaagcggtg gagagataaa cgccttttaa
    21001 cgcttcatac atggcgctca ccatcatccc gctttcactt cccggtacgt ccagcccgtc
    21061 ggcacgtccg gtatggtgcc aggcgcgagc ggcaaagtta ggggtaaaca ggcccgtgag
    21121 atagagttgt cctgtgccgt cggtaatttc cagaccattc atccaggtga tgccttcggc
    21181 aaggtcatac tggatgtaac cgttcatcca ttgccggata ggtgtatttg tcgtcgtagc
    21241 gatcgtactt gccaaaatgt agccaggctt tgctttcgtc atgatgtccg gcaggtgtgg
    21301 cggtgacgct ataacgaaag ttgccgctta agttctgtac cttcagcgcg gtcataatgt
    21361 cgcgggtatt gggaatgacg tagcccaaat cgggtgtgaa gtcgccccac cattgcaggt
    21421 catccagcga ggaatcctga cgcagactga gcatgatttc ggtgccatca ttggtacgat
    21481 aaccgccata gaaacggtta atcccacctt cgaaaccacc ccagctatgg tcaggcacct
    21541 aggcattacc gtcgtggtcg gcctgcacgg tccagccttc gccgtaaatc aggccgaacc
    21601 aggagccgta cttcatctcc aggccacctt caatataggt gccgtcgctg tatttatgtt
    21661 tattctcacc ctctaaatcc atatatccac caagccctaa ttcgccgtaa aaacgaaatg
    21721 aaggagaaga tgtcgaggta attgcgggtg tttttgtgtc gtcatgttct gccggacgtg
    21781 cttcttctcc tgccgcagaa aaggcgtagc aaaacggcag agcaagtaat gctctgcatg
    21841 gtgcagtgat taatttcatt gtgttaatcc atgagctaaa gaaaaagtac agccttttat
    21901 cgttgtaatt ataaaaatat aaaggcagaa actattttcc ctctcctcat ccaggaagag
    21961 ggaaaatgcc tgctcatttt ttattagtct gcttaatcaa cattgctggc tggcgtcgct
    22021 tcaatatggc gctcttcagc cgttaatccc tttatatatt tgctgtattt ccaccacgca
    22081 aagccgagga agataacaat accgccgacg ttataaaaaa tgatggtcag aatattcgcg
    22141 ccagtcggga atgtcgaagc gacaaaccct acggcaaata tcgcaatcag cattgaaaca
    22201 acgataatac cggtgcggcg ggagcccata cggaaatcgc gtggcaggtg atctaatttg
    22261 gcgcgtaaat tcaggtaagc cagcatgatg aataacggcg gaagcatgga cgctgcggcg
    22321 gtcatattaa taatggtatt catcagatcc tgcacggtat tggaaccgag catcgggata
    22381 atcatcagcg ggatgacgat cagaaactgg atccatgctg cgcgcgccgg aacgccgttt
    22441 tcgttaagtt cgacggtttt cttaccaaag atgccttccg ggatttcgga gaagaaaatt
    22501 ttcaccggcg ttgcggtcca catcagcagg gaaccgaaca ttgcggtaaa ggacaccaga
    22561 ccgacaaagc gattcatcaa cgcttccggc agaccaaaat acgccgccat gccgtggaat
    22621 acctgcaccg atccgccggt aaatttcaac tctttgctgc tgacgaagac gttaatcagc
    22681 accgaggaga cggaatacag tacgccgata aaaatcccgg cgaggatgat cactttaacg
    22741 aacgatttcg aaccgccttt gacgtcgtta acgtacaccg cgacggactc cgcgccacct
    22801 gcggccataa agatccaggt ggtaacgccg aggaacgccc agttgaagtt cgggatcatc
    22861 gcgtcaacgg tgatcggatc ggcaggctgt acgccgccaa ccagtgccgt acctgccagt
    22921 aaaatgtagg agagcgttaa cagcagcatc agcgttgaag tgacggaggt aattggcccc
    22981 agcattttcg ccccgttggt ggaaacccag gtggagaagg cgaacagcac catactgata
    23041 acggtggtgg caaccggcgt cataatatat tcgtagccga ggaaggcata cgaagcataa
    23101 gcaataacgc gcggcaacag cgaggtgaaa aagaacagat ttacgaacca gtaagtgtag
    23161 gcagtaataa atgcccaatg tccgcccagc gaacttttta cccacgcgta gacaccggct
    23221 tctgagtttt tatttaacga aacaaattct gcgatgatca gacagaaggg aataaaataa
    23281 aaaatcgtcg cgaggaaaaa catcggtgcc gaggccagtc caagctcaat attattattg
    23341 ataacgttgt taaagctgta aacggcggca aaagtcagcg agagcaagcc gaatttgccg
    23401 attgtattac gtttggtatc agacataaca catctccgaa aatgtgtagg gtagcggtgg
    23461 cggcaaactc cttttgccgc cgtagttgtt atttgttatg gaaataacca tcttcgatgg
    23521 tgactttgag aaccactttt ttgaccgcgt tattgcagat aaaccgatac gcttcatgga
    23581 tatcgcaaat aacgatttgc ccttcgtgga cctctacggt ttctccgcag ccttttaaat
    23641 attcacggtc agtttcatca cgataatatt ccactacctg taatgtctct ttcggcgcat
    23701 attcaatttt ttgctgccct tgcaggtagt aatgcacttc aaaatagcgt cgatgcccgg
    23761 taaatagcgc atcggtcgcg gagtcatttt ccacacggta ggtcaatgaa tcaccgatgg
    23821 agtgggcgac gccaggctga atgttgtcga tattttcaat cgcttcaacg cagcgttgcc
    23881 acttcttgcc agaggcgtaa atctggcgga actgttctaa gttatcgatg atcctcattg
    23941 cttattctcg ctgtgcaaat tcgtggaaaa gaaccctgcg ccgaactcat acgacgccag
    24001 gctttgcgcg gtagcttctc cgccagaaac cggcagcaac gtaaagccgt agctgaagtc
    24061 acggaaccag acgcgccagg agtccagcac ctcgctgccc caggagttgg agccgaggcc
    24121 aagtagctgg tggtcgaggt tcagggtgat gtcatcactg cgctgcagct cgttacagtg
    24181 ctgggcagcg tggatgtttt cctgggtata gtgccaggcg ctgaagttaa ttgggcgctg
    24241 cggaaccacc agcagaccgt tgccgtggcg gttagtcagt gccgtccagc ggacatgctg
    24301 acggttgccg ttgttctgcg ggaagggata gttctcgaac atggcatcga cggtgctgcg
    24361 ccagatatcg atgatgttag cctgctggct gtcggcgtag ttttcgcccg gtccacgacc
    24421 gtaatacgcc acctgatcgt attcgccgtt aatccccatg gtgaaaccga tgcacggaat
    24481 gatgtgcgga tagtcgccgt aacgctcgcc ggaaagcgcc acgttaacct ggccatcggc
    24541 agcgatgcgc cagatgtagg tacagcgcat cccgaagtca aacaccggcg gtgcaataac
    24601 tgtgcggctg atgatcaaca cttcaccatc gctctgttct acggcaaagt cgcgcagatg
    24661 ttcctgcatg atctgcaaat gattcggctg ccacaaccct tcgtactcct gcttgtggtt
    24721 gtcgatcatc ggcttgaaga agttgatctt tggctcgcgg gtcagcagcg actcgccatt
    24781 tacctggcag gatgtcggtt tgccactcat ttttgagaag gtgatcgcga agttgtagcc
    24841 gcgaacggtg cagctcaaac gatcgtcttc cagcgtcagc ggacgcgcat tatttggtgc
    24901 gaaaggcact ggctgcgcgg tgttttcctt cagcgggaac tgataagtgg cgatagaatg
    24961 cccggcttcg ctgtagcggg tgcgggaatc tttggtcacc gtaatgttga ggaacgcttc
    25021 gcgggcgtcc agctgcggca gcgtgatctg caagggggct tcgctgttcg gcgcaacgtc
    25081 gcgcagttta atctgctgcg tcgcgagcgt ttcaccttcg gcgcgcacct ctgcgtgcag
    25141 ggtgtagtca tcaagcgtgg taaaccacag tttattttcg actttcaact cgccgcgagt
    25201 cagcgcgtgg atttttaccg gcgcgataac ctgtttgtac tctttcaggc ccgatcccgg
    25261 cgtctgatcg gaatagatca aaccatcaag acagaagtta tagttgttgg gatagtcgcc
    25321 gtagtcgccg ccgaatttat accagacatt gccgtggtcg tcctgtgcct ggatcccgtg
    25381 gtcgcaccac tcccagacat aatgaccctg aatgcaatcg tgcttataga agacgttctg
    25441 gtactccgtc agcccgcccg gtccgttccc catcgcatga gcatattcac agatgatgcg
    25501 cggcttcgga tgcgggtatt caccaaactc attcatcagc ggcacgcggg tgtacatggt
    25561 ggaaataata tcgaccactt cagcatcgcg atcttcttcg taatgaacca gtcgcgtgtc
    25621 atccagcgct ttcgccgcat ggtacatcgc gcggatgtta cagccatagc cggattcatt
    25681 gcccagcgac cagatgatga tcgacggatg gtttttctgt gcgtggatat ggcgaacaat
    25741 gcgctcgacg tagacttttt cccactgcgg atcgtcggta atacggctga tatcgccgac
    25801 attagcaaag ccgtgcgatt cgacgtcggt ttccgccatc acaaacaggc cgtagatatc
    25861 acacagttcg taaaaacgcg gatcgttcgg gtagtgagcg gtacgcacgg agttgatatt
    25921 gtgctgcttc atcaactgga gatctttctc gacgcgatcc attccaacgg cgcggccttt
    25981 gcgatgatcg ttgtcgtgac ggttgacgcc gtgcagcatc acataacggt tattgatcca
    26041 gaacagaccg tcgcgcactt tgatatcacg gaagccaacg cgttgtggca ccacttccag
    26101 aacgttgccg ttggcgtctt tcagcgtcat gaccagatgg taaagataag gggattctgc
    26161 tgaccattgc tgcggctgtt cgacagtaaa agcaaagctg gcgctggtca gtttttcaat
    26221 tgccaaatga tcaatggcgc tgctgtgcac cacgcgttcg ccatcaaaca gggtatattc
    26281 cagcgtcgtg acgacagggg aggcggcgag attttccagc accacttcgc aggaaagcgt
    26341 ggcatcgcaa taggcttcgt caaagtcggt acgcacagtg aaatcgttaa tatgcgttag
    26401 gtgttttccg accagataaa catcgcggaa gatccccgcc gaccaccaca tatcctggtc
    26461 ttccacgtag gtagagtccg cccactgcat catgcgcaca cacaacaggt tgtcgccggt
    26521 tttaaccatc gcgctgatgt caaactctgc ggtcaggcga ctgcccttgc tgaaacccac
    26581 atactgaccg ttaacgtaga cttcaaaata ggtttcgacg ccgtcaaatt taatcagcgt
    26641 ctgtttaccc tgccagccgt cgctgagggt gaaaatacgt tgataggcac cggttgggtt
    26701 atcgctgggg acaaacggca catcgatggg gaacggaaaa ccttcgtcgg tatattgcag
    26761 tttgccgtga ccttccattt gccacatggc ggggacggta atatgccccc agtcagccat
    26821 taactcagag gtgaaggctt ctggtacttg cagcggatgg tcaaaaaagt ggaaattcca
    26881 ctgactgctt aagggcagaa acaggctgct ggtttcgcgg gcaaaggtac gcgcttgcgc
    26941 aacagaatca tatgaaaaaa agtacgcacg cggcgcaagt cggttttcgt gggtgagctg
    27001 aatgttttcc cagcgattca tagggcctcc cggtagagag aaatcggaac cattcatggt
    27061 gatggtagtt tgtgtaattg ctggggggtt agtaaaattt ttactaaaaa gcagcaggta
    27121 aatgcattta ctttaactgg atcacgaaaa taaacggcag cactgtccgg caaaaatgat
    27181 tgccggacag tgtgtaaggg gatttaacgg gtcgtgccgc gcagttttaa tttgctggga
    27241 acgaagacta gcagcggcag cgcgcgacca tcgcgggctt tttcatacac caggttaacg
    27301 ccctgacttc ccatcatctc ggaatggatg cgcacggtgg agagcggcgg aaaggtaaat
    27361 cgcgcggtgg ggatatcgtt aacgctgata agcgaaatat cttgtgggat gttcaggcca
    27421 cgttcatgaa ttgcccgcag tacgccgata gcaatggaat cggaagcaac aaacagtgcc
    27481 ttcggatagt cttcccgcgc cagcatttgt tttgccagtt cataacccga cgaactggaa
    27541 aaaccgccgc gccaaatatc ctcttcgcgt accacttgtt tcagtcggcc atattccgca
    27601 aaggcgacct cacgaatatc cgccttgcca ggctcatctt caccgccaat aaaaccaata
    27661 cgattaacgc cctggttgat atagaagtcg atgatttctt tactgatgcg tgccagatcg
    27721 atatccaccg catcgtaacc gctgccgggt tcgtgaaagt cgataaaaca gatattgtcg
    27781 gtcaacgcgc tggcagcggc gcgcagggcg ggcgtgggtt tgccgacaat taaaataccg
    27841 gtgacgtttt taatgtctgg taatccgttg tgttcataac agttggtcag ttcgatcgcc
    27901 agcttttcgc actgagtttc aatgccgtgg cggatcgcca gatagtaagg atcgttgatc
    27961 tccagctcct gctggtagct gtagatagcc agaatatggt gttggttgac tgcgcctgtc
    28021 tgtagtttac gggcgctact ggtcttgtac tccagctttt cggcgatctc gagaatgcga
    28081 tgtttcgtct cttctttcac attcaatgtc ggatcgtcat ttaagaccct ggatactgtc
    28141 gccagggata cgccagcttc gattgcgatg tcttttaatg ttgccatgtt tacctttttt
    28201 actttccctg atgtgcgccc ccattgtaga ggaagtgctt atcgggtcac ctgttttcag
    28261 taaataaagc gcgtttcttt gacgctgtaa cggtaacaca gcggcttagg aatgtgatca
    28321 cgcctgcatt ctccattttt agcctacagg attgagaaaa acttttctat agtttagtaa
    28381 aattttaccc gaagggagga agcaatggaa accgtggctt acgctgattt tgcacgtctg
    28441 gaaatgcgcg tcggaaagat tgtggaagtg aaacgccatg aaaacgccga caagctgtac
    28501 atcgtacagg ttgatgtggg gaaaaaacac tgcaaaccgt gaccagcctg gtgccgtact
    28561 acagcgaaga ggagctgatg gggaaaacgg tggtggtctt atgcaatctg caaaaagcga
    28621 aaatgcgtgg tgaaacgtcg gaatgcatgc tgttgtgtgc ggaaaccgat gacggcagtg
    28681 aaagcgtgtt gttaacgccg gagcggatga tgcctgcggg cgtacgcgtc gtataaaaag
    28741 atcggatggc gacgtcgtat cgccatccga tttgatatta cgcttcttcg acacttactc
    28801 gcatggccgc cagcgcctta cgcgccgctt tgatcaccag ttcacactgt tcaatggtca
    28861 gtgtcagtgg cggttcaatg cggatcgttt tggcgttatt gagcgttccg gccaccagta
    28921 cgcgctggcg gaacatctcg ctggcaaagt tatagccgat ttcgttatca acaaactcaa
    28981 tcgccatcaa catcccttta ccacgcgctt cctgtaccag atcgggatat tcccgcgcca
    29041 gttgacggaa accgtccagc aacatatcgc ctttttgctc agcctgagcc ggtaagttct
    29101 gctccagcaa cacattgatg gtcgccagcg ccgccgcaca ggccagcggg ttgccgccaa
    29161 aggtggtggt atgcaggaat gggttgtcga acagaactga aaacacctct tcagtggcga
    29221 tggtcgcgcc aatcggcatc acgccgccgc cgagggcttt tgccagacac agaatgtccg
    29281 gctgaacgtt ctcatgttcg caggcaaaca tcttgcccgt gcgccccatg cccgtttgta
    29341 cttcatcgag gatcatcagt gcgccgaact catcgcacag cttacgcacg gcggtgagat
    29401 agcccggcgg cggcagaatt acgccacctt cgccctgaat cggttcgagg atcaccgcag
    29461 ccacatcatc accggttttt ttgcactcat taagagccgt gcgcatggct tcgatattgc
    29521 caaacggcac atgacggaac cctggcagca gtggcataaa cggtttgcgg aaggtcgatt
    29581 tcgccgtggc cgacagcgcg ccaagtgatt taccgtggaa cgcgccgctg gtggcaataa
    29641 aagtaaattt gccgcgcggc gactgataag ccttcgccag cttcagcgcc gcttcaacgg
    29701 actcggtgcc gctattacag aagaagctgt atttcagttt accgggcgtt agcgcagcaa
    29761 gggttttcgc cagcatagcc cgtaacggat cgagtagctc ctggctgtgc agtggttgtt
    29821 tcgcaagttg attctgtacg gcggaaacca caactggatt acggtgcccc acgttgaaaa
    29881 ttccaaaacc tcccaggcag tcgataaact cctgtccctg ggtgtcgaca agcgtattta
    29941 aacctcccgc ttgccactct acggctccgt aatccccgcc ggcggtaaca gatttgcgat
    30001 actctaaaaa ccccggattg acatgctctt tgaagtattc aatcacctct cggttaagtg
    30061 ctttcatctc ctcatgatcc agcgttcgct tctcaatgag attcagggcg tgggcgctgc
    30121 acgctaaagc cgatgcgctc gaaggtaacc tgttcaaaat atgctccgga ggctcgcgta
    30181 tcacatgata ccgatttaag tattgcaggg attgcgccac tccggctgcg atcgcgaaaa
    30241 tcgggataaa cacaaatgcg gttatcattt gtgcaacatt taatgctatc cgttaacatt
    30301 tgttgttatt ttgcacggtt tttgtcgggt atggatttga tgattgctgc gcaaatgcac
    30361 gttttcaggg caataattgc ctgatttttg ggcaagataa ttaacgtttt cgtatagtaa
    30421 cagagtgaaa gtattttaaa tcaaagaatt agatttgtgc tttaatcgca aattgcgatc
    30481 taaatcaaat taatcggtta aagataaccg cagcggggcc gacataaact ctgacaagaa
    30541 gttaacaacc atataacctg cacaggacgc gaacatgtct tctcatccgt atgtcaccca
    30601 gcaaaatacc ccgctggcgg acgataccac tctgatgtcc actaccgatc tgcaaagcta
    30661 tatcactcat gctaatgaca cttttgtgca ggtgagcggc tttaccttgc aagagttaca
    30721 agggcagccg cacaacatgg tgcgtcaccc ggatatgcca aaagcggcgt ttgcggatat
    30781 gtggttcacc ctgaaaaaag gggagccctg gagcggcatc gtgaaaaatc gccgcaaaaa
    30841 tggcgaccat tattgggtgc gggccaatgc ggtaccgatg gtgcgcgagg gaaaaatcag
    30901 tggctatatg tcgattcgta cccgggcgac ggatgaagag attgcggcgg tggagccgct
    30961 gtacaaagcg ttgaactccg gacgtaccag taagcgtatt cataaaggcc tggtggtgcg
    31021 taaaggctgg ctgggtaaac tgccttcatt accgcttcgc tggcggacgc gtggagtgat
    31081 gaccctgatg tttatcttgc tggcggccat gctttggttt gttgctgccc cggtggtgac
    31141 gtatttcctc tgtgtgttag tggtattgtt ggcaagcgct tgttttgaat ggcagattgt
    31201 gcgcccgata gaaaatgtcg cccgtcaggc actgaaggtg gcgaccggag agcgtaatag
    31261 tgttgagcac ctgaatcgca gcgatgagct ggggctgaca ttacgcgcgg tagggcagct
    31321 tggcctgatg tgccgttggc taattaacga tgtctcaagc caggtgtcca gtgtcagaaa
    31381 tggcagtgag acgctggcga aaggcaccga tgaactgaac gaacataccc agcagacagt
    31441 tgataacgtt cagcaaacgg tggcgaccat gaaccaaatg gcggcgtcgg tgaaacagaa
    31501 ctctgccacg gcgtcggctg ccgataaact ttctatcacc gccagtaatg cggcagtgca
    31561 gggtggggag gcgatgacca cggtgatcaa gacaatggac gatatcgccg acagtaccta
    31621 gcgcattggc accattactt cgctgattaa cgatattgcg tttcagacca atattctggc
    31681 cctgaatgcg gcggtggaag cggcgcgtgc cggcgaacag ggcaaaggtt ttgcagtggt
    31741 ggcaggggaa gtgcgtcatt tagccagccg cagcgctaat gctgccaacg atattcgcaa
    31801 gctgattgat gccagtgctg ataaggtgca atccggttcg cagcaggtac acgccgccgg
    31861 acggacgatg gaagatattg tggcacaggt gaaaaacgtc acccagttga tcgcctagat
    31921 tagccattca acgctggaac aggccgatgg gctttccagc ctgacccgtg cagtggatga
    31981 gcttaacctg atcacccaga aaaatgccga gctggtggaa gagagtgcgc aggtgtcggc
    32041 gatggtgaaa caccgcgcca gccgactgga agacgcggtg acggtgctgc attaatcggt
    32101 atgccggatc cggcggttgg agcacaatgc ctgatgcgat gctggcgcat cttatcaggc
    32161 ctgcggggtg tgtagcgggt gtaggccgga taaggcgcgt caagcgtcgc atccggcaat
    32221 tgcaccgcgc cactggcgga tgcggcgatt aatccagttg cgtaatatca aaagcggcgc
    32281 ggtcgatcac cgcaatgatc ttttttattt gtgcatcact gatatcgctc tggttgacgc
    32341 gtaaatccag caccgcttta aaattatcca gcgcccgctt catttgcggg ttctggcgca
    32401 gcgccgcgcc aacgcaacgc gctttgatgc gttcttcaat catctccact tgttcgcggt
    32461 tttcttccag ccactgcgcg ccttgttcgg tcagcgcaat ctgcttctta ccaccttcct
    32521 cttcgcggat ggtaatcagc gactgctcct gcagaaaatc cagcgtcggg tagatgacgc
    32581 ccgggcttgg ggtgtaattc ccctgggtta gattctcaat cgctttaatc aattcgtaac
    32641 cgtggctgtc atcgcgcgag agaatatcca gaatcaccag acgcaattca ccgtgaccaa
    32701 agaaacgttg ccgacgaccg ccgccgcgac cgtggcgacc accgcaacat tgaccgtgtt
    32761 catgctggtg accgtgtccg cagtgctcgt gttctgactt ctcacctttg cagcagccct
    32821 catggcgtgg ctggccttca tgtttacaac acccttcgtg atgatggctc atatcagcct
    32881 ccttttgctt gattagatat atctaattta tatctgatgt gagcattact gcaagtacaa
    32941 aaatgatttt taaacgtgga agcacctcac atctaggccg gataagacgt tcacgccgca
    33001 tccggcagca ttgctatggc actgaatgcc ttcacatact tcagacatat cagttctaca
    33061 aatcgcttgc atttatcatg attaacaatc attatcattt gcgaatttta tttagatata
    33121 tctgattacc atcacgaagg cgataacaat gaataacacc ccccgctacc cgcagcgcgt
    33181 tcgcaatgat ctgcgcttcc gtgaactgac tgtgctacgc gttgagcgca tcagcgccgg
    33241 ttttcagcgc attgtccttg gcggcgaggc gttggacggt tttacatcgc gcggctttga
    33301 cgatcacagc aaactcttct ttcctcaacc tgacgctcac tttgtgccgc caacggtaac
    33361 ggaagagagc atcgtctggc cggaaggacc acgcccaccg tcgcgtgact atacgccact
    33421 gtatgacgaa ctacgccatg aactggcgat cgatttcttt attcacgacg gtggggtcgc
    33481 cagcggctgg gcgataaact tacggtggca ggtccgcgcg gttcgctggt ggtgccggaa
    33541 gattacgcgt atcagctgta tgtctgcgat gaatccggaa tgcccgcact gcgccgccgc
    33601 ctggaaacgt tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt tagcgtgcgg
    33661 gataacgcct gtcaggatta tctcgcgcac cttgatggtt ttaatatcga atggctggca
    33721 catgatgagc aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc cgatgattac
    33781 ttcatctgga taaccggcga aggtaaagtc gttaagaatt taagccgccg ctttgaagcg
    33841 gaacagtatg acccacagcg ggtacgtgca gcggcttact ggcacgcaaa gtaactgata
    33901 tataaaaaga aaaaggctga cgatttctcg tcagcctttg ctatatctgg tggcccctgc
    33961 tggacttgaa ccagcgacca agcgattatg agtcgcctgc tctaaccact gagctaaggg
    34021 gccatggtag cggattataa agtaactccg tgtcgcaatc cagccattac cgcgcgcctg
    34081 ctgtttttat aaacaatgta ttttcaatcc gttatacttt tcctggtgat gttagaaagg
    34141 agtaaatatg gttgaggata ttttggctcc agggttacgg gtcgtgtttt gcggtatcaa
    34201 ccctgggctt tcatccgccg ggactggttt tccctttgct catccggcaa atcgcttctg
    34261 gaaggtgata tatcaggccg ggtttaccga ccgtcagttg aagccgcagg aggcacagca
    34321 tctgctggat tatcgttgtg gcatcaccaa actggtagac cgtccaacgg tgcaagccaa
    34381 tgaagtttca aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa aaattgaaga
    34441 ttatcagccg caggcgttgg cgattctggg caaacaagca tatgaacagg gattcagcta
    34501 gcgcggtgca cagtggggga aacaaacgct caccattggt tcgacgcaga tttgggtgct
    34561 gccaaatccc agcggtttaa gtcgcgtttc actggagaaa ctggttgaag cgtatcgcga
    34621 gctggaccag gcgctggtag tgcgtgggcg ataaaa
//
LOCUS       DAJVPP010000035        34368 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVPP010000035 DAJVPP010000000
VERSION     DAJVPP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.035"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..333)
                     /locus_tag="OQM34_001675"
     CDS             complement(<1..333)
                     /locus_tag="OQM34_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452161.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            703..1653
                     /gene="corA"
                     /locus_tag="OQM34_001676"
     CDS             703..1653
                     /gene="corA"
                     /locus_tag="OQM34_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS1452162.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1694..2074)
                     /locus_tag="OQM34_001677"
     CDS             complement(1694..2074)
                     /locus_tag="OQM34_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCS1452163.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2088..2504)
                     /locus_tag="OQM34_001678"
     CDS             complement(2088..2504)
                     /locus_tag="OQM34_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452164.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="OQM34_001679"
     CDS             complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="OQM34_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1452165.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3505..3972)
                     /locus_tag="OQM34_001680"
     CDS             complement(3505..3972)
                     /locus_tag="OQM34_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1452166.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4137..5006
                     /gene="pldA"
                     /locus_tag="OQM34_001681"
     CDS             4137..5006
                     /gene="pldA"
                     /locus_tag="OQM34_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1452167.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDASAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5139..6968
                     /gene="recQ"
                     /locus_tag="OQM34_001682"
     CDS             5139..6968
                     /gene="recQ"
                     /locus_tag="OQM34_001682"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1452168.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7032..7652
                     /gene="rhtC"
                     /locus_tag="OQM34_001683"
     CDS             7032..7652
                     /gene="rhtC"
                     /locus_tag="OQM34_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS1452169.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="OQM34_001684"
     CDS             complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="OQM34_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1452170.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8445..9467
                     /gene="pldB"
                     /locus_tag="OQM34_001685"
     CDS             8445..9467
                     /gene="pldB"
                     /locus_tag="OQM34_001685"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1452171.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9475..10275
                     /gene="yigL"
                     /locus_tag="OQM34_001686"
     CDS             9475..10275
                     /gene="yigL"
                     /locus_tag="OQM34_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1452172.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10351..11250
                     /gene="bioP"
                     /locus_tag="OQM34_001687"
     CDS             10351..11250
                     /gene="bioP"
                     /locus_tag="OQM34_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1452173.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11138..12091)
                     /gene="metR"
                     /locus_tag="OQM34_001688"
     CDS             complement(11138..12091)
                     /gene="metR"
                     /locus_tag="OQM34_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1452174.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12327..14588
                     /gene="metE"
                     /locus_tag="OQM34_001689"
     CDS             12327..14588
                     /gene="metE"
                     /locus_tag="OQM34_001689"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1452175.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14627..15442)
                     /locus_tag="OQM34_001690"
     CDS             complement(14627..15442)
                     /locus_tag="OQM34_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1452176.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15704..16465
                     /gene="udp"
                     /locus_tag="OQM34_001691"
     CDS             15704..16465
                     /gene="udp"
                     /locus_tag="OQM34_001691"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1452177.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16606..18033
                     /gene="rmuC"
                     /locus_tag="OQM34_001692"
     CDS             16606..18033
                     /gene="rmuC"
                     /locus_tag="OQM34_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1452178.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18128..18883
                     /gene="ubiE"
                     /locus_tag="OQM34_001693"
     CDS             18128..18883
                     /gene="ubiE"
                     /locus_tag="OQM34_001693"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1452179.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18897..19502
                     /gene="ubiJ"
                     /locus_tag="OQM34_001694"
     CDS             18897..19502
                     /gene="ubiJ"
                     /locus_tag="OQM34_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1452180.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19499..21139
                     /gene="ubiB"
                     /locus_tag="OQM34_001695"
     CDS             19499..21139
                     /gene="ubiB"
                     /locus_tag="OQM34_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1452181.1"
                     /translation="MTLGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEARFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21218..21487
                     /gene="tatA"
                     /locus_tag="OQM34_001696"
     CDS             21218..21487
                     /gene="tatA"
                     /locus_tag="OQM34_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1452182.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21491..22006
                     /gene="tatB"
                     /locus_tag="OQM34_001697"
     CDS             21491..22006
                     /gene="tatB"
                     /locus_tag="OQM34_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1452183.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22009..22785
                     /gene="tatC"
                     /locus_tag="OQM34_001698"
     CDS             22009..22785
                     /gene="tatC"
                     /locus_tag="OQM34_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1452184.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22827..23609
                     /gene="tatD"
                     /locus_tag="OQM34_001699"
     CDS             22827..23609
                     /gene="tatD"
                     /locus_tag="OQM34_001699"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1452185.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="OQM34_001700"
     CDS             complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="OQM34_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1452186.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24261..25754
                     /gene="ubiD"
                     /locus_tag="OQM34_001701"
     CDS             24261..25754
                     /gene="ubiD"
                     /locus_tag="OQM34_001701"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1452187.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25800..26501
                     /gene="fre"
                     /locus_tag="OQM34_001702"
     CDS             25800..26501
                     /gene="fre"
                     /locus_tag="OQM34_001702"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1452188.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26693..27856)
                     /gene="fadA"
                     /locus_tag="OQM34_001703"
     CDS             complement(26693..27856)
                     /gene="fadA"
                     /locus_tag="OQM34_001703"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1452189.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27866..30055)
                     /gene="fadB"
                     /locus_tag="OQM34_001704"
     CDS             complement(27866..30055)
                     /gene="fadB"
                     /locus_tag="OQM34_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS1452190.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERSKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30245..31576
                     /gene="pepQ"
                     /locus_tag="OQM34_001705"
     CDS             30245..31576
                     /gene="pepQ"
                     /locus_tag="OQM34_001705"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1452191.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31576..32190
                     /locus_tag="OQM34_001706"
     CDS             31576..32190
                     /locus_tag="OQM34_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1452192.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32229..33680
                     /gene="trkH"
                     /locus_tag="OQM34_001707"
     CDS             32229..33680
                     /gene="trkH"
                     /locus_tag="OQM34_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1452193.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33692..34237
                     /gene="hemG"
                     /locus_tag="OQM34_001708"
     CDS             33692..34237
                     /gene="hemG"
                     /locus_tag="OQM34_001708"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1452194.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8081 a         8684 c         9284 g         8319 t
ORIGIN      
        1 cttctgctga ccgttttcga tgtacaaacc gagcggcgca tagctttcat catagatgcc
       61 gccgttcatc gccatctgca cctgaccctg actattaata tccgccagca gagcatgtaa
      121 cgttccccac gcttcgccat tggctttttg ccagtacatt ttcacccgct ctgtttgagg
      181 attaacggta tacgcctgta cggtcagcgt cggatctgag agtgcgcaat catcagcggc
      241 aacagcaaac aagggaagta aggtgagggt gagaaaaatc cgtttgagat tcaaggtgat
      301 cattccttta ccaatgagta gccgatgcgc cattataggt cctagatgtg ggattttttt
      361 atcctgttag cgaccttgac gagtaccaaa aagcgcgaag ttcaactatt gttctgtggt
      421 gttctgttgc gtgttgacgg caaaattttg ctggcgtaac atgcgcgcac gatcactcta
      481 agaggacatt cgccttggac acacccagta gatactggct cactatcctg tcatccagga
      541 tcaactccta aggctatccc tttttgctga tagccttagc ggttgtcagc gacctcaatt
      601 tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa cccaatttgt ttctgtgtgc
      661 ccaccgaact gtccgatatt ttaagcattg ggagtcccgg tcatgctgag cgcatttcaa
      721 ctggaaaata accgactgac ccggctggaa gtcgaagagt cacaacccct tgtaaatgca
      781 gtatggattg atcttgtcga accggacgac gacgagcgac tgcgcgtaca atctgaactt
      841 ggccagagcc tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt
      901 gaagacgacg acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac
      961 gccggtaact ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag
     1021 cgtgaactgc ccgcttttcg tctgtatcgt atgcgtgccc gtagccagtc gatggtagac
     1081 ggtaacgcct acgagttgct gctggatctg ttcgaaacca aaatcgaaca gttggcagat
     1141 gaaattgaaa atatctatag cgacctggag cagttgagcc gggtgattat ggaagggcat
     1201 cagggcgatg agtacgacga ggcgctctcc acgctggcgg aactggaaga tatcggctgg
     1261 aaagttcgcc tgtgtctgat ggatacccag cgcgctctca acttcctggt gcgtaaagcg
     1321 cgtttaccgg gtgggcaact ggagcaggcg cgtgaaatcc tgcgcgatat cgaatccctg
     1381 ctgccgcata acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcaatgggt
     1441 tttatcaaca tcgagcagaa ccgcatcatc aaaattttct cggtggtatc cgtcgtgttc
     1501 ctgccgccaa cgctggttgc ttccagctac gggatgaact ttgagtttat gccagaactg
     1561 aagtggagtt tcggctaccc tggcgcgatt atctttatga tcctcgcggg cctggcaccg
     1621 tatctgtact ttaagcggaa gaactggttg taaaaagaag gcggtggctt agcctagcta
     1681 agccacctgt tattcaaagg ctccaggtat ttaacccttt tacctctttc tcataaaacc
     1741 atttgttcgt gttaacagca acataggctg ctacggcaat tcccagaatg ttaacgccaa
     1801 ttaatgcacc aacaaataga gatagaatgc caagtaatag aactataatt gcttttttcc
     1861 ataagcccag aacaaataaa tagatccagg aacagaagaa agcaatgaaa ttcatttgaa
     1921 tagttaatcg ttgtcttact tttaatgctt taaatgctgc tttatattct ggtgttgctc
     1981 cccagaatcc aggaaagcca tgttgatcat aaaaattaaa tcggtatttc catttttcac
     2041 ttaatgaatc atcgttcata tattccttac tcataaatac tccataacta ttgttttgat
     2101 gaatcagtag gtgcaagcat tagcatactg aaagtggaaa aataacaaat caaaaaaatc
     2161 atcgaaccat tgcctgaaca ggcaaaatct tcggctatca ttgtgatgat agagatgata
     2221 tatactgcta atgtaccaaa aacataaatt tttatatata tgaaaccact atcacggagt
     2281 cgttggcaat tcatgttgat gacgagataa tggagtgcga tggtagagac tataacaaga
     2341 aaacctgctt ccccatcgtt aaaaaagata ataagaaagg caaaaatgaa atttattaaa
     2401 ataaataaaa atatatatcg gtgtctggaa atcttaccgt tagatgttgg gataaacata
     2461 cgtaacataa attttacatc cttgtgtgag tctcctgtca gcatggcaat atgcccactc
     2521 tcatacaaga gtcggcatat tttttcagaa tatatttatt ttttatttgg acgttctacg
     2581 ctgcgtataa atcgcgtcca tcacaaaaat tgccagcgcc acccaaataa aggtgaaagt
     2641 caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag
     2701 cgtcgggcca atgtactgaa aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc
     2761 agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag
     2821 cgacatcggg ttttgcccca tatggctggt tgaactgtcg gcaatagcaa acaggtaaat
     2881 cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc
     2941 aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat
     3001 aggtagcgaa ccaaaagtcc acagctggac caacacgcca catatcgcca gaatcaccgc
     3061 cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac
     3121 cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca
     3181 gataaacagt agccagttgc caccaatcag tacggcagag actgccagca taaaaatttt
     3241 ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac
     3301 caccataaag aaaaacgacc agatcacgcg atgcgtcagg atctcatcgg cgggcacgta
     3361 gtaaatcagc ttgaagtatg ctggcgcaat accccaaata aaataagcgg caagagcgag
     3421 taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac
     3481 agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat
     3541 ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg
     3601 caacaggcta ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg
     3661 aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc
     3721 gtggcgggtt aaggtacttc ccacgcacac cagaccggcg gcgacatcca gcgccgacgc
     3781 aatgactccg ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg gctgattttt
     3841 aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca atgcgcggtt
     3901 aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag
     3961 tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt ttatgcttct
     4021 ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa
     4081 atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc
     4141 ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag
     4201 aggcaacggt gaaagaggtg catgacgcgt cagcggtgcg tggcagtatt atcgccaata
     4261 tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct
     4321 acacccaaac cagcgatctg aataaagaag cgattgccag ttacgactgg gcggaaaatg
     4381 cgcgtaagga tgaagtaaag tttcagttga gcctggcatt tccgctgtgg cgtgggattt
     4441 taggcccaaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca
     4501 atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt
     4561 ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc
     4621 acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc
     4681 tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata
     4741 ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc
     4801 acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg
     4861 gcgcggagtt aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt
     4921 acagcggcta tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtag
     4981 gggttatgct aaacgacttg ttttgatgaa cggttgagtg gttggcaaat ctggaatcca
     5041 gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa
     5101 aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga
     5161 atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc
     5221 ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg gttgttatgc
     5281 ccactggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta
     5341 ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctacaggcca
     5401 acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg cgaacagcaa cttgaagtga
     5461 tgacaggctg ccgcaccggg caaattcgct tactgtatat cgccccggaa cgcctgatgc
     5521 tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc gtcgatgaag
     5581 cgcactgtat ctcccaatgg ggccacgatt tccgcccgga atatgccgcg ctcggtcagt
     5641 tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca
     5701 cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt
     5761 ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga
     5821 tgcgctacgt gcaggaacag cgcggtaagt ctggcattat ctactgcaac agccgggcga
     5881 aagtagaaga caccgctgcg cgcctgcaaa gcaggggtat tagcgcggcg gcctatcatg
     5941 ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgatctgc
     6001 aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg
     6061 tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg
     6121 ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc
     6181 tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac
     6241 tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact
     6301 attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga
     6361 aacagtacga cggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc
     6421 agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc
     6481 gcgactatgg tcatgacaaa ctgaaagtct atggtatggg ccgtgataaa agccatgaac
     6541 attgggtgag cgtgatccgc cagctgattc atctcggcct ggtgacgcaa aatattgccc
     6601 agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt
     6661 tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg
     6721 gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg
     6781 atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg
     6841 aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc
     6901 tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat ggcgacgacg
     6961 aagagtagtc agcagcataa aaaagtgcca gtatgaagac tccgtaaacg tttcccccgc
     7021 gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt gtggcgctta
     7081 tgagccccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag
     7141 aagcgatgat gggcgtgctg ggcattacct gcggcgtaat ggtttgggct gggattgcgt
     7201 tgcttggcct gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggtag
     7261 gcggtggcct gtatctctgc tggatgggtt accagatgct acgcggtgca ctgaaaaaag
     7321 aggctgtatc tgcgcctgca ccacaggttg agctggcgaa aagcgggcgc agtttcctga
     7381 aaggcttact gaccaatctc gccaatccga aagcgattat ctattttggt tcggtattct
     7441 cattgtttgt cggcgataac gttggcacta ccgagcgctg gggcattttc gcgttgatta
     7501 ttgtcgaaac actggcgtgg tttaccgtcg ttgccagtct gtttgccctg ccgcaaatgc
     7561 gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg
     7621 gatttggcat tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg
     7681 ataaggcgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag
     7741 cgctcccacc agcataaaca acgagccgaa aatcttattc agcgccttca tctgctttgg
     7801 tcctttaatc catagagcaa tcctttgagc aagggtggcg taaccgatca tcacaataat
     7861 atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg gctgttgcgg
     7921 catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt
     7981 cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag
     8041 atcaattgcg ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc
     8101 tgcccacttt aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac
     8161 gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc
     8221 gccgcgataa ccgtggttga gcgaggtggt catagtgttg attgcaccag agcctggcga
     8281 caggcttaaa atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa
     8341 ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac
     8401 gcttaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa
     8461 aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt
     8521 tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg
     8581 tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga
     8641 gctacgtaaa atatgcggaa ctggcctacg acctgttcca tttggggttt gatgtcttaa
     8701 tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc
     8761 atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc
     8821 agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca
     8881 cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt
     8941 ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag
     9001 cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt
     9061 ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg
     9121 ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc
     9181 tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgcaaacg cttctcttac
     9241 aagctgaaga ggaacgcgtg gtggataacc gcatgcacga ccgtttttgt gaactccgca
     9301 ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt aataaaaggt gcttaccatg
     9361 agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatc gttgattttt
     9421 tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac
     9481 caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct
     9541 tacgccaaag aaactctgaa gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc
     9601 ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg
     9661 attacctcca atggtgcgcg cgttcacgat ctggatggca atctgatttt tgctcataac
     9721 ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt
     9781 actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc
     9841 ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc cgggattact ggagccggaa
     9901 ggcgtcagca aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag
     9961 gcgattaacg ctcgctgggg cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg
    10021 gaagtgatgg cgggcggcgt gtcgaaaggc catgcgctgg aagcagtggc gaagaaactg
    10081 ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg
    10141 tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agatctgcat
    10201 ccggagctgg aagttattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa
    10261 ctctatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat
    10321 ggatactcgt taatcaaaga gttttccatt gtggcgttac ttatcatcac cacgattctg
    10381 tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt
    10441 gcggtgctgg tgcgcgttgg cttggcggca ctcgtttttc tgccgtttct gcgtacccgt
    10501 agcaatagcc tgaaaacggt cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg
    10561 atgtatatgc tgagtttccg cgcttatctc tacctgacgg tctccgaatt gttgctgttc
    10621 accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg
    10681 cgctggggct atgcttttag cgccttgctg gcggtgattg gtgccgggat tattcgctat
    10741 gatcaggtca ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact
    10801 tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagtat
    10861 aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg
    10921 ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt
    10981 ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac
    11041 gccggaacgc tgggcattat gaataatatg cacgttccgg cagggctgct ggtaaacctg
    11101 gctatctggc accaacagcc gcactggcca acgtttatta caggcgcgct ggtgatcctg
    11161 gcctcactgt gggtgcatcg taagtgggtc gctccgcgct cttcacaaac ggcagatgat
    11221 cgcaggcgtg attgcgcgct gagcgaataa acgcttccgt aactggctga cgctgctcgc
    11281 catcgcgcac ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca
    11341 ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc
    11401 gcgcggcaac catttgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga
    11461 cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa
    11521 ttaatagcgt ctcgctggcg agatcttccg gtgtaatacg cgtttttgcc gccagtggat
    11581 ggtcaggtgc taacaccagg cgtacttcat aatcgaacat cggcgaataa tgcaggccac
    11641 tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct
    11701 gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga
    11761 aattttctaa agcgggtgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca
    11821 gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctgatttgc ggcagcacct
    11881 ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac
    11941 gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg
    12001 attgcgtctg atgcaacgtt gccgcagcgg ctgcgagcga gccgcagttc cgcaacgctt
    12061 gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt
    12121 tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa
    12181 aatcattcgt cttttaaatt tatggtgcgt tggctgcgtt tctccacccc ggtcacttac
    12241 ttcagtaagc tcccggcgat gaataaactt gccgccttcc ctaaattcaa aatccatagg
    12301 atttacatat aattagagga agaaaaatga caatattgaa tcacaccctc ggtttccctc
    12361 gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca
    12421 cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa
    12481 agcaagcggg tatcgacctg ctgccggtgg gcgattttgc ctggtacgat catgtactga
    12541 ccaccagttt gctgctgggt aatgttccgc cacgtcatca gaacaaagac ggttcggtag
    12601 atatcgacac cctgttccgt attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg
    12661 cagcggaaat gaccaaatgg tttaacacca actatcacta catggtgccg gagttcgtta
    12721 aaggccaaca gttcaaactg acctggacgc agctgctgga ggaagtggac gaggcgctgg
    12781 cgctgagcca caacgttaag cctgtgctgc tggggccggt tacttacctg tggctgggga
    12841 aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa cgacattctg ccggtttatc
    12901 agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg
    12961 cgctggtact ggaactacca caggcgtggc tggacgcata caaacccgct tacgacgcgc
    13021 tccagggaca ggtgaaactg ctgctgacca cctattttga aggcgtaacg ccaaatctcg
    13081 acacgattac tgcgctgcct gttcaggggc tgcatgtgga ccttgtacat ggtaaagata
    13141 acgttgttga actgcacaag cgcctgcctt ctgactggct gctgtctgcg ggtctgatca
    13201 atggtcgtaa cgtctggcgc gccgatctta ctgagaaata tgcgcaaatt aaggacattg
    13261 tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt gctgcacagc cccatcgacc
    13321 tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg gtttgccttc gccctacaaa
    13381 aatgccatga actggcactg ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg
    13441 cagagtggag cgccccaatt caggcgcgtc gtcactctac tcgtgtacat aatccggcgg
    13501 tagaaaagcg tctggcggcg atcaccgccc aggacagcca gcgtgcgaat gtctatgaag
    13561 tgcgtgctga agcccagcgt gcgcgtttta aactgccagc gtggccgacc accacgattg
    13621 gttccttccc gcaaaccacg gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc
    13681 tcgacgccaa caactaccgc acgggcattg cggaacatat caagcaggcc attgttgagc
    13741 aggaacgttt gggactggat gtgctggtac atggcgaggc cgagcgtaat gacatggtgg
    13801 aatactttgg cgagcacctc gacggatttg tctttacgca aaacggttgg gtacagagct
    13861 acggttcccg ctgcgtgaag ccaccgattg tcattggtga cattagccgc ccggcaccga
    13921 ttaccgtgga gtgggcgaag tatgcgcaat cgctgaccga caaaccggtg aaagggatgc
    13981 tgacggggcc ggtgaccata ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa
    14041 ccatcgccaa acagattgcg ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg
    14101 gaattggcat catccagatt gacgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta
    14161 gcgactggga tgcgtatctc cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg
    14221 cgaaagatga cacacaaatc cacactcaca tgtgttattg cgagttcaac gacatcatgg
    14281 attcgattgc agcgctggac gcagacgtca tcaccatcga aacctcgcgt tccgacatgg
    14341 agttgctgga gtcgtttgaa gagtttgatt atccaaatga aatcggtcct ggcgtctatg
    14401 acattcactc gccaaacgta ccgagcgtgg aatggattga agccttgctg aagaaagcgg
    14461 caaaacgcat tccggcagag cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg
    14521 gctggccaga aacccgcgcg gcactggcga acatggtgca ggcggcgcag aatttgcgtc
    14581 gggggtaaat ccaaaccggg tggtaatacc acccggtctt ttctcattac agcgacttct
    14641 tcccaccata ctgcttaaac cattccagca tacgctgcca gccatctttc gcagattcgg
    14701 catgatagct cgggcgataa tcagcgttga atgcatgccc ggcatccggg tacacgataa
    14761 tctctgcttt cgcattagca gcccgcagcg cctggcgcat cgtttcaacg ctctcctgcg
    14821 gaatgctgtt atcctgacca ccatataagc cgagaaccgg cgcgttaaga tcggttgcga
    14881 tatcaacagg ttgtttcggt gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg
    14941 ccactgcggc ttttagctgt ggattatgcg cggcatacag ccaggtgata cgtccacccc
    15001 agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg
    15061 cgacatgatc gagatcggcc agcacctgcg agtcaggcac ttttgctacc agaccgctaa
    15121 gcaacgtggg gatatcggca aaatcattcg gatcgccttc gcggaagtaa agttcaggtg
    15181 cgatagccag atacccctcc agcgccagac ggcgacaaat gtcgcggata tgttcatgca
    15241 cgccaaaaat ttcctgcact acaatgacca ctggcagtgg gccatcgctt tgctttggtc
    15301 tggcatggta agcaggcatg ttatcccctt gtgaagggat agaggtgaag cccgccacaa
    15361 tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc
    15421 cagattgttg tgttgttgcc atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt
    15481 taactatagt cagcatgcaa caaatcacat tgcctgaatc ggctcatctt ttatgcagtc
    15541 ctgcagaatg aagagtgatt tatgtgatat gaatcacttt tggtgggtaa atttatgcaa
    15601 cgcatttgcg tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc
    15661 tttttgcttc ttctgactaa accgattcac agaggagttg tatatgtcca agtctgatgt
    15721 ttttcatctc ggcctcacta aaaacgattt acaaggggct acgcttgcca tcgtccctgg
    15781 cgacccggat cgtgtggaaa agatcgccgc gctgatggat aagccggtta agctggcatc
    15841 tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc
    15901 taccggtatc ggcggcccgt ctacctctat tgctgttgaa gagctggcac agctgggcat
    15961 tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag ccgcatatta atgtgggtga
    16021 tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct
    16081 ggaattcccg gctgtcgctg atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc
    16141 tattggcgcg acaactcacg ttggcgtgac agtttcttct gataccttct acccaggtca
    16201 ggaacgttac gatacttact ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga
    16261 gtggcaggcg atgggcgtaa tgaactatga aatggaatct gcaactctgc tgaccatgtg
    16321 tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt atcgttaacc gcacccagca
    16381 agagatcccg aatgctgaga cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt
    16441 ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc
    16501 cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt ccaactggac gtttgtacag
    16561 cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc
    16621 atggtttacg cagttattgc gttggtgggt gtggcaattg gctggctgtt tgccagttat
    16681 caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg aagagatggt cgcggagtta
    16741 agcgcggcaa aacaacaaat tacccaaagc gagcactggc gtgcagagtg tgaattgctc
    16801 aataacgaag tgcgcagcct gcaaagtatt aacacctctc tggaggccga tctgcgtgaa
    16861 gtaaccacgc ggatggaagc cgcacagcaa catgctgacg ataaaattcg ccagatgatt
    16921 aacagcgagc agcgcctcag tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac
    16981 agcaatcgcc gggttgatga gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta
    17041 cgtgaacaac tggacggttt ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa
    17101 gaacgccata ccctgaccca cgaaattcgc aatctccagc aactcaacgc gcaaatggcc
    17161 caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg
    17221 ggcgaggtag tattgacgcg ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat
    17281 gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg
    17341 cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa
    17401 cgctatttta acgccgaaga cgactacacc cgcgaaagcg cgctacagga acatatcgcg
    17461 tcggtgcgta accatatccg tttgctggga cgcaaagatt atcaacagct gccggggctg
    17521 cgaactctgg attacgtgct gatgtttatt cccgttgaac ccgctttttt actggcgctt
    17581 gaccgccagc cggagctgat caccgaagcg ttgaaaaaca atatcatgct ggttagcccg
    17641 actacgctgc tggtggcgtt gcgtactatt gccaacctgt ggcgttatga gcatcaaagc
    17701 cgtaacgccc agcaaatcgc cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc
    17761 atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag
    17821 gcaatgaaaa aactctcttc aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc
    17881 ggtttaggag tagaaattaa acgcgagatt aatccggatt tggctgaaca ggcggtgagc
    17941 caggatgaag agtatcgact tcggtcggtt ccggagcagc cgaatgatga agcttatcaa
    18001 cgcgatgatg aatataatca gcagtcgcgc tagcccatta ggggtagtta agccgggtag
    18061 aaatctaggg catcgacgcc caatctgtta cacttctgga acaatttttt gatgagcagg
    18121 cattgagatg gtggataagt cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa
    18181 ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc gtggcatcaa aatacgatgt
    18241 catgaatgat ttgatgtcat ttggtattca tcgtttgtgg aagcgattca cgattgattg
    18301 cagcggcgta cgccgtgggc agaccgtgct ggatctggct ggtggcaccg gcgacctgac
    18361 agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg gtccttgctg atatcaatga
    18421 atccatgctc aaaatgggac gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga
    18481 gtatgttcag gcgaacgctg aggcgctgcc gttcccggat aacacctttg attgcatcac
    18541 catttcgttt ggtctgcgta acgtcaccga caaagataaa gcactgcgtt caatgtatcg
    18601 cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc
    18661 gctgagcaaa gcctatgatg catactcctt ccatgtgctg ccgcgtattg gctcactggt
    18721 cgcgaacgac gccgacagct accgttatct ggcagaatcc atccgtatgc atcccgatca
    18781 ggataccctg aaagccatga tgcaggatgc cggattcgaa agtgtcgact actacaatct
    18841 gacggcaggg gttgtggcgc tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc
    18901 cttttaaacc tttagtgacg gcaggaattg aaagtctgct caacaccttc ctgtatcgct
    18961 cacccgcgct gaaaacagcc cgctcgcgtc tgctgggtaa agtattgcgc gtggaggtaa
    19021 aaggcttttc gacgtcattg attctggtgt tcagcgaacg ccaggttgat gtactgggcg
    19081 aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc
    19141 gcgatcgcca gcagcttacc gcactgattc gcagtggtga gctggaagtg cagggcgata
    19201 ttcaggtggt gcaaaacttc gttgcgctgg cagatctggc agagttcgac cctgcggaac
    19261 tgctggcccc ttataccggt gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag
    19321 gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg ttatgtggcg gaagccatta
    19381 ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg
    19441 ctgccgtcga gcgtgctgtt gatgccctga ccaaacggct ggaaaaactg gaggctaaat
    19501 gacgctaggt gaagtacggc gcctatattt catcattcgc acttttttaa gctacggact
    19561 tgatgaactg atccccaaaa tgcgtatcac cctgccgcta cggctatggc gatactcatt
    19621 attctggatg ccaaatcggc ataaagacaa acttttaggt gagcgactac gactggccct
    19681 gcaagaactg gggccggttt ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct
    19741 ttttccaccg catattgccg atcagctggc gttattgcag gacaaagttg ctccgtttga
    19801 tggcaagctg gcgaagcagc agattgaagc tgcaatgggc ggcttgccgg tagaagcgag
    19861 gtttgacgat tttgaaatca agccgctggc ttctgcttct atcgcccagg ttcataccgc
    19921 gcgattgaaa tcgaatggta aagaggtggt gattaaagtc atccgcccgg atattttgcc
    19981 ggtcattaaa gcggatctga aacttatcta ccgtctggct cgctgggtgc cgcgtttgct
    20041 gccggatggt cgccgtctgc gcccaaccga agtggtgcgc gagtacgaaa agaccttgat
    20101 tgatgaactg aatttgctgc gggaatctgc caacgccatt cagcttcggc gcaattttga
    20161 agacagcccg atgctctaca tcccggaagt ttaccctgac tattgtagtg aagggatgat
    20221 ggtgatggag cgcatttacg gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg
    20281 cactaacatg aaattgctgg cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg
    20341 cgacagcttt ttccatgccg atatgcaccc tggcaacatc ttcgtaagct atgaacaccc
    20401 ggaaaacccg aaatatatcg gcattgattg cgggattgtt ggctcgctaa acaaagaaga
    20461 taaacgctat ctggcagaaa actttatcgc cttctttaat cgcgactatc gcaaagtggc
    20521 agagctacac gtcgattctg gctgggtacc accagatacc aacgttgaag agttcgaatt
    20581 tgccattcgt acggtctgtg aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg
    20641 acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc aatatggaag tgcagccgca
    20701 actggtgtta ctccagaaaa ccctgctcta tgtcgaaggg gtaggacgcc agctttatcc
    20761 gcagctcgat ttatggaaaa cagcgaagcc tttcctggag tcgtggatta aagatcaggt
    20821 cggtattcct gccctggtga gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat
    20881 gccagaactg cctgaactgg tttacgacag tttgcgccag ggcaagtatt tacagcacag
    20941 tgttgataag attgcccgcg agcttcagtc aaatcatgta cgtcagggac aatcgcgtta
    21001 ttttctcgga attggcgcta cgttagtatt aagtggcaca ttcttgttgg tcagccgacc
    21061 tgagtggggg ctgatgcccg gctggctaat ggcaggtggt ctgatcgcct ggtttgtcgg
    21121 ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg
    21181 gctttgttta atcatcatct accacagagg aacatgtatg ggtggtatca gtatttggca
    21241 gttattgatt attgccgtca tcgttgtact gctttttggc accaaaaagc tcggctccat
    21301 cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc
    21361 aaagcaggat aaaaccagtc aggatgctga ttttactgcg aaaactatcg ccgataagca
    21421 ggcggatacg aatcaggaac aggctaaaac agaagacgcg aagcgccacg ataaagagca
    21481 ggtgtaatcc gtgtttgata tcggttttag cgaactgcta ttggtgttca tcatcggcct
    21541 cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa acggtagcgg gctggattcg
    21601 cgcgttgcgt tcactggcga caacggtgca gaacgaactg acccaggagt taaaactcca
    21661 ggagtttcag gacagtctga aaaaggttga aaaggcgagc ctcactaacc tgacgcccga
    21721 actgaaagcg tcgatggatg aactacgcca ggccgcggag tcgatgaagc gttcctacgt
    21781 tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc atccataacc cggtggtgaa
    21841 agataatgaa gctgcgcatg agggcgtaac gcctgccgct gcacaaacgc aggccagttc
    21901 gccggaacag aagccagaaa ccacgccaga gccggtggta aaacctgctg cggacgctga
    21961 accgaaaacc gctgcacctt ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa
    22021 gatactcaac cgcttatcac gcatctgatt gagctgcgta agcgtctgct gaactgcatt
    22081 atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg ccaatgacat ctatcacttg
    22141 gtatccgcgc cattgatcaa gcagttgccg caaggttcaa cgatgatcgc caccgacgtg
    22201 gcctcgccgt tctttacgcc gatcaagctg acctttatgg tgtcgctgat tctgtcagcg
    22261 ccggtgattc tctatcaggt gtgggcattt atcgccccag cgctgtataa gcatgaacgt
    22321 cgcctggtgg tgccgctgct ggtttccagc tctctgctgt tttatatcgg catggcattc
    22381 gcctactttg tggtctttcc gctggcattt ggcttccttg ccaataccgc gccggaaggg
    22441 gtgcaggtat ccaccgacat cgccagctat ttaagcttcg ttatggcgct gtttatggcg
    22501 tttggtgtct cctttgaagt gccggtggca attgtgctgc tgtgctggat ggggattacc
    22561 tcgtcagaag acttacgcaa aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg
    22621 atgttgctga cgccgccgga tgtcttctcg caaacgctat tggcgatccc gatgtactgt
    22681 ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa
    22741 gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag aataaattca accgcccgtc
    22801 agggcggttg tcatatggag tacaggatgt ttgatatcgg cgttaatttg accagttcgc
    22861 aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc
    22921 tactcatcac cggcactaac ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt
    22981 attcgtcctg ttggtcaacg gcgggcgtac atcctcacga cagcagccag tggcaagctg
    23041 cgactgaaga agcgattatt gagctggccg cgcagccaga agtggtggcg attggtgaat
    23101 gtggtctcga ctttaaccgc aacttttcga cgccggaaga gcaggaacgc gcttttgttg
    23161 ctcagctacg cattgccgca gaattaaaca tgccggtatt tatgcactgt cgcgatgccc
    23221 acgagcggtt tatgacattg ctggagccgt ggctggataa actgcctggt gcggttcttc
    23281 attgctttac cggcacacgc gaagagatgc aggcgtgcgt ggcgcgtgga atttatatcg
    23341 gcattaccgg ttgggtttgc gatgaacgac gcgggctgga gctgcgggaa ttgttgccgt
    23401 tgattccggc ggagaaattg ctgatcgaaa cagatgcgcc gtatctgctc ccgcgcgatc
    23461 tcacgccaaa gccatcatcc cggcgcaacg agccagccca tctgccccat attttgcaac
    23521 gtattgcgca ctggcgtgga gaagatgccg catggctggc tgccaccacg gatgccaatg
    23581 tcaaaacact gtttgggatt gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt
    23641 gcttaatctc tttattaata agattaagca atagcatgga gcgagcctca ccatcgggtt
    23701 cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt aataatcacc ttatcacccg
    23761 gataaggggg ggccggatcg acaatgtctt tcggtttata taccgatagc tgatgaatta
    23821 ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac aaagtggctg acgccacgcg
    23881 tagcgctgat ggtcgtggta tgaatcactt ctggatcaaa ttccacaaac aggtagttag
    23941 ggaataacgg ttcactgact gcggtacgtt taccccgtac gattttttcc agggtgatca
    24001 tcggagcgag gcaattaacc gcctgtctct caaggtgttc ctgggcacgt tgaagttgcc
    24061 cgcgcttgca gtacagtaaa taccaggatt gcataatgac tcttatccgt ttaatcgggg
    24121 cgcaaggata gcaaaagctt tacgctaagt taattatatt ccccggtttg cgttataccg
    24181 tcagagttca cgctaattta acaaatttac agcattgcaa agatgaacgc cgtataatgg
    24241 gcgcagatta agaggctaca atggacgcca tgaaatataa cgatttacgc gacttcctga
    24301 cgctgcttga acagcagggt gagctaaaac gtatcacgct cccggtggat ccgcatctgg
    24361 aaatcactga aattgctgac cgcactctgc gtgctggtgg gcctgcgctg ttgttcgaaa
    24421 accctaaagg ctactcaatg ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg
    24481 cgatgggcat ggggcaggaa gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt
    24541 ttctgaaaga gccggagccg ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt
    24601 ttaagcaggt gttaaacatg ccgacaaagc gactgcgtgg tgcgccctgc caacaaaaaa
    24661 tcgtctctgg cgatgacgtc gatctcaatc gcattcccat tatgacctgc tggccggaag
    24721 atgccgcgcc gctgattacc tgggggctga ccgtaacgcg cggcccgcat aaagagcggc
    24781 agaatctggg catttatcgc cagcagctga ttggtaaaaa caaactgatt atgcgctggc
    24841 tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg tgcggcgcat ccgggcgaac
    24901 gtttcccggt ttctgtggcg ctgggtgccg atcctgccac gattctcggt gcagtcaccc
    24961 ccgttccgga tacgctttca gagtatgcgt ttgccggatt gctacgcggc accaaaaccg
    25021 aagtagtaaa gtgtatttcc aatgacctcg aagtgccagc cagtgccgaa atcgtcctgg
    25081 aagggtatat cgatcctggt gagatggcgc cggaagggcc gtatggtgac cacacaggtt
    25141 actataatga agtcgatagt ttcccggtgt ttaccgtgac gcatattacc cagcgtgaag
    25201 atgcgattta ccattccacc tataccgggc gtccgccaga tgagcccgcg gtactgggcg
    25261 tggcgttgaa cgaagtgttt gtaccgattc tgcaaaaaca gttcccggaa attgtcgatt
    25321 tttacctgcc gccggaaggc tgttcttatc gtctggcggt agtgacgatc aaaaaacagt
    25381 acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt
    25441 acactaaatt tgtgatcgtt tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga
    25501 tttgggcgat taccacccgt atggacccgg cgcgggatac tgttctggta gaaaatacgc
    25561 ctattgatta tctggatttt gcctcgcctg tctctgggct gggttcaaaa atggggctgg
    25621 atgccacgaa taaatggccg ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag
    25681 atccagatgt tgtcgcgcat attgacgcca tctgggatga actggctatt tttaacaacg
    25741 gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc gatccgacag agaaagcgca
    25801 tgacaacctt aagctgtaaa gtgacctcgg tagcagctat cacggatacc gtatatcgtg
    25861 tccgcatcgt gccagacgcg gccttttctt ttcgtgctgg tcagtatttg atggtagtga
    25921 tggatgagcg cgacaaacgt ccgttctcaa tggcctcgac gccggatgaa aaagggttta
    25981 tcgagctgca tattggcgct tctgaaatca acctttacgc gaaagcagtc atggaccgca
    26041 tcctcaaaga tcatcaaatc gtggtcgacc ttccccacgg agaagcgtgg ctgcgcgatg
    26101 atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg gttctcttat gcccgctcga
    26161 ttttgctgac agcgttggcg cgtaacccaa accgtgatat caccatttac tggggcgggc
    26221 gtgaagagca gcatctgtat gatctctgcg agcttgaggc gctttcgtta aagcaccttg
    26281 gtctgcaagt ggtgccggtg gttgaacaac cggaagcagg ctggcgtggg cgtactggta
    26341 ccgtgttaac ggcggtattg caggatcatg gtacgctggc agagcatgat atctatattg
    26401 ccggacgttt tgagatggcg aaaattgccc gtgacctgtt ttgcagtgag cgtaatgcgc
    26461 gggaagatcg cctgtttggc gatgcgtttg catttatctg agatataaaa aaacccgccc
    26521 ctgacaggcg ggaagaacgg caactaaaac tttattcagt ggcatttaga tctatgtcgc
    26581 atccggcaat tggtgcacga tgcctgatgc gacgctggcg cgtcttatca ggcctacatt
    26641 ggtgccggat cggtagaccg gataaggcgt tcacgccgca tccggcaagt ggttaaaccc
    26701 gctcaaacac cgtcgcaata ccctgaccca gaccgataca catcgtcgcc agaccaaact
    26761 gaacgtcttt gcgttccatc agattcagca gcgtggtgct gatacgcgca ccggaacaac
    26821 ccagcggatg acccagcgcg atcgcaccac cattgaggtt gatcttctcg tcaatctgct
    26881 ccattagccc gagatcttta atgcacggca ggatctgcgc ggcaaaggct tcgttcatct
    26941 caaacacgcc gatatcgctg gcagaaagcc ccgctttttt cagcgccagc ttcgaggccg
    27001 gaaccgggcc gtaacccata atcgatgggt cacaaccaac gaccgccatc gaacgcacac
    27061 gagcgcgcgg cttaagacct aattcatggg cgcggctttc actcatcacc agcatggcag
    27121 ctgcgccatc ggaaagtgca gaagatgtgc ccgccgttac cgtaccgttt actggatcaa
    27181 acgccggacg cagcgtggcg agggcttcca cggtggtttc cgggcgaatc acttcgtcgt
    27241 aattaaactg cttcaggacg ccgtcggcat cgtgaccacc agttgggatg atttcatttt
    27301 taaatgcggc cgactgcgtg gcggcccagg cgcgggcgtg tgaccgcgcg gcaaaggcat
    27361 cctgcatttc acggctgata ccgtgcatac gcgccagcat ttctgccgtt aagcccatca
    27421 tgcccgccgc tttggcgaca ttgcggctca ggccggggtg aaaatcgacg ccgtgactca
    27481 tcggcacatg gcctatatgc tccacgccgc caaccagaca tgcctgcgca tcgccagtca
    27541 tgatcattcg tgctgcgtca tgcagtgcct gcatggatga accacacaag cgattgacgg
    27601 taaccgccgg gacagagtgc ggcacttctg ccagcagcgc cgcgttacgg gcgatgttaa
    27661 aaccctgctc cagcgtctgt tgcacacaac cccagtaaat atcgtcgagg gccgccgctt
    27721 ccagcgccgg gttacgcgcc agcaggctac gcattaaatg agcggagaga tcttccgcac
    27781 gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg ggtgcgaatt gcatcgacaa
    27841 tgacaacctg ttccattgtg actccttaag ccgttttcag gtcgccaacc ggacgggctg
    27901 gctcaaccgg aggatagtac ggttcgttat gacgcgcttt attacgcaga ccttccggca
    27961 cttcatacag cgggccgagg tgctgatatt gctgtgccat atcgaggtat tttgcgctac
    28021 cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg agggaagccc aggccgtaga
    28081 ccagcgccat atccgcttcc gccggagtgg cgataatgcc ctcttccaga cagcgcacca
    28141 cttcgttgac catcgggatc atcatgcggg cgataatctc ttcttcgctg aaatcgcgct
    28201 tcggttggct cacttctgcc agcaggtctt caacggcggc gtcttcttct ttcttcggct
    28261 tacctttgct gtcttcttta taacgccaga aaccgaggcc gttcttctga ccaaagcggt
    28321 tggcatcaaa cagcgcgtcg atggcatcgc ggtaatcttt ctgcatccgc tgcgggaagc
    28381 ctgctgccat gacagcctga gcgtgatgcg cggtatcaat gcccacaacg tccagcagat
    28441 atgccgggcc catcggccag ccaaactgtt tttccatcac tttgtcgatc ttgcggaaat
    28501 ccgcgccgtc gcgcagcagc tggctgaaac cggcgaaata cgggaacagc acgcggttaa
    28561 caaagaagcc ggggcagtcg ttaaccacaa tcggcgtctt gcccatcttg ctcgcccagg
    28621 cgacaacttt cgcgatggtt tcgtcggagc ttttctcgcc gcgaataatt tctaccaacg
    28681 gcattcggtg gaccgggtta aagaagtgca tcccgcagaa gttttccggg cgttccagcg
    28741 cgttggccag ttcgctgata ggaatggttg aagtgttaga cgccagcacg gtatcctggc
    28801 gtactttttg ttcggtttct gccagtacgg cttttttcac tttcgggttt tcaacaaccg
    28861 cttctaccac aatatccacg cggtcaaatc cggcgtagtc gagcgttggg tggatcgtgg
    28921 agatcacgcc agccagtttc agaccatcga ttttgctgcg ctcaagctgc ttgttcagca
    28981 gtttcgcggc ttcggtcatg ccgagggtta acgacttgtc gttgatatct ttcatgacaa
    29041 ccggcacgcc tttccacgcg gactggtaag cgataccgcc acccatgatg cctgcgccta
    29101 gcacagcagc ctgtttcggg gtttcaacgt ctttggtgag tttcttcgct ttgcctttta
    29161 catattgatc gttaaggaaa atgccgacca gtgcgcgagc ttcgttggta tgcgccagcg
    29221 ggacaaaact tttgttttcc agatttaagg cttcttcacg accaaaacgg gccgcagctt
    29281 caatggtttt tactgcggtg atgggggccg gataatgttt ccccgccgtt tgcgcgacca
    29341 ttcctttagc gatggtgaag ctcatggtgg cttcaatctt gctcagtttt agcggttcct
    29401 gcttcggctg acgtttcgct ttccagtcga ggtcgccgtt aatggcctgg cgtaaaatcg
    29461 ccatcgcccc ttcaatcagt ttttctgctt tgactacgcc atccaccaga ccgattttca
    29521 gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat ttccagtgca ctgtcagcgc
    29581 ccagcatacg tggcatacgt acagaaccgc caaagcccgg catgatgccc agtttggttt
    29641 ccggcagacc gatgcgcaga tccggcgtcg ccagacgata atcggtcgcc agcacgcatt
    29701 cgcagccacc acccagcgca tagccgttga cggcagcaat ggtcggcacc ggcagatctt
    29761 ccaggcgatt aaacacgcta ttggcaaagt gcagccactg acttaactgt tcttcaggaa
    29821 cgaggaacag ggacaaaaat tcggtgatat cagcaccgac gataaaggct gctttgttcg
    29881 aacgcagcag cagccctttt agatctgatt gctgttccag cacgccgatg gcctcgccga
    29941 ggctggcgac ggtcgcagtg tcgagtttat taactgaacc tggggcatca aataccagtt
    30001 cggcaatgcc atcttccagc cagtcaaggt acagggtgtc gcctttgtaa agcatgtcag
    30061 tctcctgaat ccgcaaggtg atctggtcgt accagatgca tcggagtgtg tgttttgtgt
    30121 taaaaatatg caaataaaag attaaagaaa tgccgatctg atcacgctcg gcagaaatca
    30181 cgctctggat gaacgatgtg ctaagatgcg gagacttaag gtcaaaaaaa cagaagggta
    30241 aaaaatggaa tcactggcct cgctctataa aaatcatata gctaccttac aagaacggac
    30301 tcgcgatgcg ctggcgcgct tcaagctgga tgcattactt attcactccg gcgagctgtt
    30361 caacgttttt ctcgacgatc atccctatcc gtttaaagtg aacccgcaat tcaaagcgtg
    30421 ggtgccggta actcaggtgc caaactgctg gttgctggtg gatggcgtga acaagccgaa
    30481 actgtggttc tatctgccgg ttgattactg gcacaacgtc gaaccgctgc cgacctcctt
    30541 ctggactgaa gatgtggaag tgatcgcgct gccgaaagcc gatggcattg gtagcctgct
    30601 gcctgctgcg cgcggcaata tcggttatat cggtccggtg ccggaacgtg cgctgcaact
    30661 gggtattgag gccagcaaca tcaacccgaa aggggtgatc gactacctgc attattaccg
    30721 ctccttcaaa accgagtacg aactggcctg tatgcgtgaa gcgcagaaaa tggcggtcaa
    30781 tggtcatcgt gcggcagaag aagcgttccg ttctggcatg agcgagttcg atatcaatat
    30841 tgcctatctg accgcgaccg gtcatcgtga taccgacgta ccttacagca acattgtggc
    30901 gcttaacgaa cacgctgcgg tgttgcatta caccaaactg gaccaccagg caccggaaga
    30961 gatgcgcagc ttcctgctgg atgccggggc cgaatataac ggctatgcgg ctgacctgac
    31021 ccgtacctgg tcggcaaaaa gcgacaacga ctacgcacag ctggtgaaag acgtaaatga
    31081 tgaacaactg gcgctgatcg ccaccatgaa agcaggcgtc agctatgtgg attaccacat
    31141 ccagttccat cagcgcatcg ccaaactgct gcgtaaacat caaatcatca ccgatatgag
    31201 tgaagaagcg atggtcgaaa acgatctcac cggaccgttt atgccgcacg gtattggcca
    31261 tccgctgggc ctgcaggtgc atgacgtcgc cggttttatg caggatgata gcggtacaca
    31321 cctcgcggca ccggcaaaat atccgtacct gcgctacacc cgtattctcc agccgggcat
    31381 ggtgttaacc atcgaaccgg gtatctactt cattgaatcg cttctggcac cgtggcgtga
    31441 agggcagttc agcaagcact tcaactggca gaaaattgaa gcactgaaac cgttcggcgg
    31501 cattcgtatc gaagacaacg tggtgatcca cgaaaacaac gtggaaaaca tgacccggga
    31561 tctgaaactg gcgtgatgga aagctggtta attcctgcgg caccggtcac ggtcgttgaa
    31621 gagatcaaaa agagccgctt cataacgatg ttggcgcata ccgatggcgt tgaggcggcg
    31681 aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg cccgccacca ttgcgtggcg
    31741 tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt tctctgacga cggggagccg
    31801 gcgggaacgg caggtaaacc gatgctcgcc cagctaatgg gcagcggcgt cggggaaatt
    31861 accgctgtgg tagtgcgcta ctacggcggc atattgcttg gcaccggtgg gttagtgaaa
    31921 gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa cgacccaacg caagacgcca
    31981 ttaaccgaat atactttgca atgtgaatat agtcagttaa ccggcattga agcgttgctg
    32041 gggcagtgtg acggcaaaat tatcaacagt gattatcagg cattcgttct gctgcgggtg
    32101 gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc tggcggattt tagccgtggt
    32161 tcattgcaat tgttagcgat tgaagaataa tccccacttc gttttgcaga actaaggaag
    32221 cggcagagat gcattttcgc gccattaccc gaatcgttgg actactggtc atcttatttt
    32281 cagggaccat gattatccct gggctggtag cactcatcta ccgggatgga gcgggccgcg
    32341 cttttaccca gacctttttt gtcgccctcg ccattggctc tatgctgtgg tggccgaacc
    32401 gcaaagagaa gggcgaactg aaatcccgtg aggggtttct gatagtggtg ctgttttgga
    32461 ccgtgctggg tagtgtcggt gcgctccctt ttatcttctc ggaaagcccg aacctcacga
    32521 ttaccgatgc gttttttgaa tctttctctg gcctgaccac caccggggcc actacgctgg
    32581 tggggctgga ttcgctccct catgccatcc tcttttatcg ccagatgctg caatggtttg
    32641 gcgggatggg gatcatcgtg ttagcggttg cgatactgcc tatcctcggc gtgggtggga
    32701 tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga taacaaaatg cgcccgcgaa
    32761 ttgcggaaac ggcgaaaact ctgtggttga tttatgtctt gctgaccgtc gcctgtgcgc
    32821 tggcgttgtg gtttgccgga atggatgcct ttgatgccat cggccatagc tttgcgacta
    32881 tcgctattgg cggcttctcg acacatgatg ccagtatcgg ttatttcgac agtccgacta
    32941 ttaacactat cattgctatc ttcctgctga tctccggctg taactacggt ctgcactttt
    33001 cactgttaag tgggcgtagt ctgaaggttt attggcgcga tccggaattt cgcatgttta
    33061 tcggcgtaca gtttacgctg gtggttattt gtacactcgt actgtggttt cataatgtct
    33121 acagttcggc gctgatgaca attaaccagg cgtttttcca ggtggtatcg atggcgacaa
    33181 ccgccgggtt tacgactgac agcattgccc gctggccgct ctttttgccg gtactgcttt
    33241 tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg cggtggcctg aaagtgatcc
    33301 gcatcctgct gctgtttaag caggggaacc gtgagctgaa acgactggtg catccgaacg
    33361 ccgtctatag cattaagctg gggaatcgcg cactgccgga acgtatcctc gaagccgttt
    33421 ggggattttt ctccgcctat gcattggtgt ttattgtcag tatgctggcg attatcgcca
    33481 cgggcgtgga tgatttttct gcctttgcct cagttgttgc gacattgaat aacctggggc
    33541 cagggcttgg cgtggttgct gataacttta ccagtatgaa cccggtggct aaatggatcc
    33601 tgattgccaa catgctgttt ggtcgtctcg aggtctttac attactggtg ctctttaccc
    33661 cgactttctg gcgtgaatga tggagtaata cgtgaaaaca ttaattcttt tctcaacaag
    33721 ggacggacaa acgcgcgaga ttgcctccta cctggcttcg gaactgaaag aactggggat
    33781 ccagacggat gtcgccaatg tgcaccgcat tgaagaacca caatgggaaa actatgaccg
    33841 tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac tctgcgttcc aggaatttgt
    33901 caaaaaacat gcgacgcgac tgaattcgat gccgagcgcc ttttattccg taaacctggt
    33961 ggcgcgcaaa ccggagaagc gtactccaca gaccaacagc tacgcgcgga agtttctgat
    34021 gaactcgcaa tggcgtcccg atcgctgcgc ggtcattgcc ggggcgctgc gttacccacg
    34081 ttatcgctgg tacgaccgtt ttatgctcaa gctgattatg aagatgtcag gcggtgaaac
    34141 ggatacgcgc aaagaagttg tctataccga ttgggagcag gtggcgaatt tcgcccgaga
    34201 aatcgcccat ttaaccgaca aaccgacgct gaaataagca taaagaataa aaaatgcgcg
    34261 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    34321 ccactgacac ggaacaacgg caaacacgcc gccgggtcag cggggttc
//
LOCUS       DAJVPP010000036        34274 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVPP010000036 DAJVPP010000000
VERSION     DAJVPP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.036"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            585..2870
                     /gene="nrdA"
                     /locus_tag="OQM34_001709"
     CDS             585..2870
                     /gene="nrdA"
                     /locus_tag="OQM34_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1452195.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3104..4234
                     /gene="nrdB"
                     /locus_tag="OQM34_001710"
     CDS             3104..4234
                     /gene="nrdB"
                     /locus_tag="OQM34_001710"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1452196.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4234..4488
                     /gene="yfaE"
                     /locus_tag="OQM34_001711"
     CDS             4234..4488
                     /gene="yfaE"
                     /locus_tag="OQM34_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1452197.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4542..5192)
                     /gene="inaA"
                     /locus_tag="OQM34_001712"
     CDS             complement(4542..5192)
                     /gene="inaA"
                     /locus_tag="OQM34_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCS1452198.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5371..5613
                     /locus_tag="OQM34_001713"
                     /pseudo
     CDS             5371..5613
                     /locus_tag="OQM34_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5655..6731)
                     /gene="glpQ"
                     /locus_tag="OQM34_001714"
     CDS             complement(5655..6731)
                     /gene="glpQ"
                     /locus_tag="OQM34_001714"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1452199.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6736..8094)
                     /gene="glpT"
                     /locus_tag="OQM34_001715"
     CDS             complement(6736..8094)
                     /gene="glpT"
                     /locus_tag="OQM34_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1452200.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8367..9995
                     /gene="glpA"
                     /locus_tag="OQM34_001716"
     CDS             8367..9995
                     /gene="glpA"
                     /locus_tag="OQM34_001716"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1452201.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9985..11244
                     /gene="glpB"
                     /locus_tag="OQM34_001717"
     CDS             9985..11244
                     /gene="glpB"
                     /locus_tag="OQM34_001717"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1452202.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLIDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11241..12431
                     /gene="glpC"
                     /locus_tag="OQM34_001718"
     CDS             11241..12431
                     /gene="glpC"
                     /locus_tag="OQM34_001718"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1452203.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12624..13574
                     /locus_tag="OQM34_001719"
     CDS             12624..13574
                     /locus_tag="OQM34_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1452204.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13615..14085)
                     /locus_tag="OQM34_001720"
     CDS             complement(13615..14085)
                     /locus_tag="OQM34_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1452205.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14140..15342)
                     /locus_tag="OQM34_001721"
     CDS             complement(14140..15342)
                     /locus_tag="OQM34_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCS1452206.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15429..15971)
                     /locus_tag="OQM34_001722"
     CDS             complement(15429..15971)
                     /locus_tag="OQM34_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452207.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16250..16675
                     /gene="nudI"
                     /locus_tag="OQM34_001723"
     CDS             16250..16675
                     /gene="nudI"
                     /locus_tag="OQM34_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1452208.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16714..17316)
                     /locus_tag="OQM34_001724"
     CDS             complement(16714..17316)
                     /locus_tag="OQM34_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS1452209.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17624..18763
                     /gene="arnB"
                     /locus_tag="OQM34_001725"
     CDS             17624..18763
                     /gene="arnB"
                     /locus_tag="OQM34_001725"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1452210.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18767..19735
                     /gene="arnC"
                     /locus_tag="OQM34_001726"
     CDS             18767..19735
                     /gene="arnC"
                     /locus_tag="OQM34_001726"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1452211.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19735..21717
                     /gene="arnA"
                     /locus_tag="OQM34_001727"
     CDS             19735..21717
                     /gene="arnA"
                     /locus_tag="OQM34_001727"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS1452212.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21714..22604
                     /locus_tag="OQM34_001728"
     CDS             21714..22604
                     /locus_tag="OQM34_001728"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1452213.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22604..24256
                     /gene="arnT"
                     /locus_tag="OQM34_001729"
     CDS             22604..24256
                     /gene="arnT"
                     /locus_tag="OQM34_001729"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1452214.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24253..24588
                     /gene="arnE"
                     /locus_tag="OQM34_001730"
     CDS             24253..24588
                     /gene="arnE"
                     /locus_tag="OQM34_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1452215.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24588..24974
                     /gene="arnF"
                     /locus_tag="OQM34_001731"
     CDS             24588..24974
                     /gene="arnF"
                     /locus_tag="OQM34_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1452216.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24968..25240)
                     /gene="pmrD"
                     /locus_tag="OQM34_001732"
     CDS             complement(24968..25240)
                     /gene="pmrD"
                     /locus_tag="OQM34_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1452217.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25345..26700)
                     /gene="menE"
                     /locus_tag="OQM34_001733"
     CDS             complement(25345..26700)
                     /gene="menE"
                     /locus_tag="OQM34_001733"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1452218.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26697..27659)
                     /gene="menC"
                     /locus_tag="OQM34_001734"
     CDS             complement(26697..27659)
                     /gene="menC"
                     /locus_tag="OQM34_001734"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1452219.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27659..28516)
                     /gene="menB"
                     /locus_tag="OQM34_001735"
     CDS             complement(27659..28516)
                     /gene="menB"
                     /locus_tag="OQM34_001735"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1452220.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28531..29289)
                     /gene="menH"
                     /locus_tag="OQM34_001736"
     CDS             complement(28531..29289)
                     /gene="menH"
                     /locus_tag="OQM34_001736"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1452221.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29286..30956)
                     /gene="menD"
                     /locus_tag="OQM34_001737"
     CDS             complement(29286..30956)
                     /gene="menD"
                     /locus_tag="OQM34_001737"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1452222.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31045..32340)
                     /gene="menF"
                     /locus_tag="OQM34_001738"
     CDS             complement(31045..32340)
                     /gene="menF"
                     /locus_tag="OQM34_001738"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1452223.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32419..32724)
                     /gene="elaB"
                     /locus_tag="OQM34_001739"
     CDS             complement(32419..32724)
                     /gene="elaB"
                     /locus_tag="OQM34_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1452224.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32779..33240)
                     /locus_tag="OQM34_001740"
     CDS             complement(32779..33240)
                     /locus_tag="OQM34_001740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1452225.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33305..34222
                     /gene="rbn"
                     /locus_tag="OQM34_001741"
     CDS             33305..34222
                     /gene="rbn"
                     /locus_tag="OQM34_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCS1452226.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         8340 a         9218 c         8530 g         8186 t
ORIGIN      
        1 atttgatgaa taatatcagt gcgtcataat tcaagttaat aaccttaagg gataccagtt
       61 atatttaaac taaattaaag taatgaataa ttttcttata atataaggta aattaacaaa
      121 atggcttcgc atttaacaat aaccgaatag aaaacaacca tttcgacatc aacaatcgct
      181 tacattcgct tatatattga ccacaactga tacatcagat tatgtgatga ctcgtgctta
      241 gatcaatttt tgcaatcatt agcaaaaaga ttaataaacc atctatatca atttatccaa
      301 cctattctgc cgttcaagaa atcgccgaac agttattttt aacaaatttt tctcttccca
      361 ttgactttcc cggacacctt gtctgaccta aggtgcgcga aagccacttt ttccttcctg
      421 agttatccac aaagttatgc acttgcaaga gggtcatttt cacactatct tgcagtgaat
      481 cccaaacata ccccctatat atagtgttct aagcagcttc ccttactaca ggtagtctgc
      541 atgaaactat tgcggaaaga attccaaaaa caggtacgac atacatgaat cagaatctgc
      601 tggtgacaaa gcgcgacggt agcacagagc gcatcaatct cgacaaaatc catcgcgttc
      661 tggattgggc ggcagaagga ctgcataacg tttcgatttc ccaggtcgag ctgcgctccc
      721 acattcagtt ttatgacggt atcaagacct ctgacatcca cgaaactatt atcaaggctg
      781 ccgcagacct gatctcccgt gatgcgccgg attatcagta tctcgccgcg cgcctggcga
      841 tcttccacct gcgtaaaaaa gcctacggcc agtttgagcc gcctgcgctg tacgaccacg
      901 tggtgaaaat ggtcgagatg ggcaaatacg ataatcatct gctggaagac tacacggaag
      961 aagagttcaa gcagatggac acgtttatcg atcacgaccg tgatatgacc ttctcttatg
     1021 ctgccgttaa gcagctggaa ggcaaatatc tagtacagaa ccgcgtgacc ggcgaaatct
     1081 acgaaagcgc ccagttcctt tatattcttg ttgccgcgtg cctgttctcg aactacccgc
     1141 gtgaaacgcg cctgcaatac gtgaagcgtt tttatgacgc ggtttccaca tttaaaattt
     1201 cgctgccgac gccaatcatg tccggcgtgc gtaccccgac tcgtcagttc agctcctgcg
     1261 tactgatcga gtgcggtgac agcctggatt ccatcaacgc cacctccagc gcgattgtga
     1321 aatatgtttc ccagcgtgcc gggatcggca tcaacgccgg acgtattcgt gcgctgggca
     1381 gcccgattcg cggtggcgaa gcgttccata ccggctgtat cccgttctac aaacatttcc
     1441 agacagcggt gaaatcctgc tctcagggcg gtgtgcgcgg cggtgcggca acgctgttct
     1501 acccgatgtg gcatctggaa gtggaaagcc tgctggtgtt gaaaaacaac cgtggggttg
     1561 aaggcaaccg cgtgcgtcat atggactacg gggtacaaat caacaaactg atgtataccc
     1621 gcctgctgaa aggtgaagat atcaccctgt tcagcccgtc cgacgtaccg gggctgtacg
     1681 acgcgttctt cgccgatcag gaagagtttg aacgcctgta taccaaatat gagaaagacg
     1741 acagcatccg caagcagcgt gtgaaagccg ttgagctgtt ctcgctgatg atgcaggaac
     1801 gtgcgtctac cggtcgtatc tatattcaga acgttgacca ctgtaatacc catagtccgt
     1861 ttgatccgac catcgcgcca gttcgccagt ctaacctgtg cctggaaatt gccctgccga
     1921 ccaaaccgct gaacgacgtc aacgacgaga acggtgaaat cgcgctgtgt acgctgtctg
     1981 ctttcaacct gggcgcaatt aatagcctgg atgaactgga agagctggcg attctggcgg
     2041 ttcgtgcact tgacgcgcta ctggattatc aggattaccc gatcccggcc gccaaacgtg
     2101 gagcgatggg gcgtcgtacg ctgggtattg gtgtgattaa cttcgcttac tatctggcga
     2161 agcacggtaa acgctactcc gacggcagcg ccaacaacct gacgcataaa accttcgaag
     2221 cgattcagta ttacctgctg aaagcgtcta atgagctggc taaagagcaa ggcgcgtgcc
     2281 cgtggtttaa cgaaaccact tacgcgaaag ggatcctgcc gatcgatacc tataagaaag
     2341 atctggatgc catcgctaat gagccgctgc attacgactg ggaagcattg cgtgaatcaa
     2401 tcaaaacgca cggcctgcgt aactccacgc tttctgccct gatgccgtcc gagacctctt
     2461 cgcagatctc taacgccact aacggtattg aaccgccgcg cggttacgtc agcatcaaag
     2521 cgtcgaaaga cggtattttg cgccaggtgg tgccggacta cgagcacctg cacgacgcct
     2581 atgagctgct gtgggaaatg ccgggtaacg atggctacct gcaactggta ggtatcatgc
     2641 agaaatttat cgatcagtcg atctctgcta acaccaacta cgatccgtca cgtttcccgt
     2701 caggaaaagt gccgatgcag cagttgctga aagacctgct caccgcctac aaattcgggg
     2761 tcaaaacgct gtattatcag aacacccgtg acggcgctga agacgcgcag gacgatctgg
     2821 taccgtcaat ccaggacgat ggctgcgaaa gcggcgcatg taagatctga tattgagatg
     2881 ccggatgcgc cgtaaacgcc ttatccggcc tacggctcgg tttgtaggcc tgataagacg
     2941 cgccagcgtc gcatcaggct ccgggtgccg gatgcagcgt gaacgcctta tccggcctac
     3001 ggctcggatt tgtaggcctg ataagacgcg ccagcgtcgc atcaggcaca ggatgcggcg
     3061 taaaatgcct tatccggcat taaactccca acaggacaca ctcatggcat ataccacctt
     3121 ttcacagacg aaaaatgatc agctcaaaga accgatgttc tttggtcagc cggtcaacgt
     3181 ggctcgctac gatcagcaaa aatatgacat cttcgaaaag ctgatcgaaa agcagctctc
     3241 tttcttctgg cgtccggaag aagttgacgt ctcccgcgac cgtatagatt accaggcgct
     3301 gccggagcac gaaaaacaca tctttatcag caacctgaaa tatcagacgc tgctggattc
     3361 cattcagggt cgtagcccga acgtggcgct attgccgctt atttctattc cggaactgga
     3421 aacctgggtc gaaacctggg cgttctcaga aacgattcat tcccgttcct atactcatat
     3481 cattcgtaat atcgttaacg atccgtctgt tgtgtttgac gatatcgtca ccaacgagca
     3541 gatccagaaa cgtgcggaag ggatctccag ctattacgat gaactgatcg aaatgaccag
     3601 ctactggcac cttctgggcg aaggcaccca caccgttaac ggtaaaaccg taaccgttag
     3661 cctgcgcgag ctgaagaaaa aactgtatct ctgcctgatg agcgttaacg cgctggaagc
     3721 gatccgcttc tacgtcagct ttgcttgttc ctttgccttt gcagaacgcg aactgatgga
     3781 aggtaacgcc aagattattc gcctgattgc ccgcgatgaa gccctgcacc tgaccggtac
     3841 tcagcatatg ctgaatctgc tgcgcagcgg cgcggatgat ccggagatgg cggaaattgc
     3901 cgaagaatgt aagcaggagt gctatgacct gtttgtccag gcggcacagc aagagaaaga
     3961 ctgggcggat tatctgttcc gtgacggttc gatgattggt ctgaataaag atattctctg
     4021 ccagtacgtt gaatacatca ccaatatccg catgcaggca gtcggtttgg atctgccgtt
     4081 ccagacgcgc tccaacccga tcccgtggat caacacctgg ctggtgtctg ataacgtgca
     4141 ggttgctccg caggaagtgg aagtcagttc ttatctggtc gggcagattg acgcggaagt
     4201 ggacaccgac gatttgagta acttccagct ctgatggccc gcgttaccct gcgcatcact
     4261 ggcacacaac tgctgtgcca ggatgaacac ccttcccttc tggcggcgct ggaatcccac
     4321 aatgtggcgg ttgagtacca gtgccgcgaa ggttactgcg gctcctgtcg cacccgactg
     4381 gtcgcaggtc aggtggactg gattgccgaa ccgttagcct ttattcagcc gggggaaatt
     4441 ttgccctgtt gttgccgggc aaaaggcgat attgaaatcg agttgtgatc gggtactagc
     4501 gccagatacg acgcttacgc gtattatctg gcctacaacg attacatggc gtagtaatac
     4561 gctttcacct gctcccagtc cgctttaggg atcggctcca gatatttttc cagctggcgg
     4621 aagtcatgat taatcgcttt atcgcgacgc aagcggcgac gacttttctc cagatcaaga
     4681 aaaccagctt ctgccttacc ttctgttttc acatagatat ggcgaacata gcaacagcca
     4741 tgctgacgat taacgctatg cattttctta aacgccagtg ccactgcttt caacatggct
     4801 tgccgtactt cgtcagaata aggcgatact gcatgccggg cataccagtc agcaatgctg
     4861 atgaaccccg ccatatcttc agtcaccagc aacgcgcgcc attcaccctc aattttcacc
     4921 gcttcaccaa aaacgatctt cggcacgatg acaccggccc gttccagttc tttaattacc
     4981 gcaacctcac ggacaatcgt tggtcggccg aacggataac gtactgaatg aaacagatga
     5041 tgcgtcatgc gctttacata cagctttttg ccgttgcgct cgacgcattg caccccgctc
     5101 ataccattac ggcgatagtt aggctcttca acccagtcgc cctctgttgc ccaccagtga
     5161 ttaaattcgt cgtactttgc tgaaactgcc ataccctatc gcctgtcatt tttattaacg
     5221 acaatgacta taggtggtta cctgaggaaa atcttaatga aacgtgtcgt attaatgaat
     5281 ttcttttgcc gctacaggtg tgacaccgct tttgcccttc atttgcagcg catagattcc
     5341 accagccact accactaata gtgcgcccag cagataaaat gtgaatttaa tcagtcggat
     5401 acagcgatga attttattcg acagggatta ggcatcgcct tacaaccaga gttaacgctg
     5461 aaaagcattg caggtgaatt gtgttccgtt cctcacgaac caaccttcta tcgacagatt
     5521 tcgttgctga ctaaagaaaa gccggtagaa ggcagtccac tgtttttact acaaatgtgc
     5581 atggaacaat tagtggcgat tggaaaaatt tgatatgaga caggatggcg catgaacgcc
     5641 atcctgcagt aacattactc tttattaaga aattttactg ccttatcagg gaaatcagta
     5701 aacagcccat ttacacccgc tttgttatac agagcatcat ataactgatt cacatcagga
     5761 gtgtattcag gcagtttatc tgaccgcacg gtataaggat gcactaccag tttattctgc
     5821 tgggcatctt gcaccatgcc agtgagtttg atattacccg gctgcgatgt ctcctcaatc
     5881 aacatatggt aatccggacc aataccatct gcatattccg ccacctgttt catggctccc
     5941 ggcttaaaca tccagtcgta gttgtaatta acccagcttc catccggctg tttctgctgc
     6001 gtttcattcc agtcggtata ggcaatcagc tgtaccagat tgagctccat gcccattttg
     6061 ggttccagct cattcttaat acgcttcagc tcatcagcat caaaacattg caaataaact
     6121 ttatcgtctt taccggtgta accatatttc ttcagcactt ccagcgtttt tgccgcaata
     6181 tccttccctt cctgatgatg gaaccacggc gctttgattt ctggatagat accgatattt
     6241 ttcccggtag agtgatttaa cccctgaaca aattcaatct cttcttcaaa ggtgtgcacc
     6301 cggaagtcgg acttacccat tgggaaacgc cccggataag tctgtacttt tttaccgttt
     6361 tcaatatcga aaccttcggt aaatttcagc gacttaattt catccagcgt gaaatctatc
     6421 gcgtagtaac gaccgtcttt gcgcgcccga tccgggaaac gatcggcaac atcagtaaca
     6481 cgatcgagat aatggtcatg cagaacaacc agatggtcgt ctttggtcat caccaaatcc
     6541 tgttccagat aatccgctcc ctgcgcatac gccatcgctt ttgctggcag cgtatgctcc
     6601 ggcaaatatc cactggcacc gcgatgggcg atgactattt tttcgttgct gtccgccgcc
     6661 attgcactgc ttcccatgac tatagtgctc atcatgatcg ccatgctaag gtttttcagc
     6721 ttcaatttca tgccattagc ctccgttgcg tttttgcagt aattgttcat ggcgacgttt
     6781 ttcgccaatc atcacaacaa tcaacaagat aaccgccaga atgctgccgc caatcattac
     6841 cataaagccg ccatcccagc cgaagaagtc cacggtgtag ccaacaatcg cgctcgccgc
     6901 caccgaaccg cccaggtaac caaacagccc ggtaaagccc gctgccgtac ctgccgcttt
     6961 tttcggtgcc agttccagcg catgcagacc gatcagcatc acaggaccgt agatcaggaa
     7021 gccgataaca atcatacaaa tcatatcgac ggttgggtta cctgccgggt tcatccagta
     7081 aacgatagtc gcgatggtca ccagtgtcat aaagaagacg ccggttgccc cacggttacc
     7141 acggaagact ttgtccgaca tccagccgca cagcagggtg cccggaatac ccgcatactc
     7201 atagaagaag taggcccagg aggatttatc cagcgcgaaa tgcttaacct ctttcagata
     7261 agtcggtgac cagtcgagga tgccgtaacg cagcagataa acgaacacgt tggcgatggc
     7321 gatgtaccac agcagtttgt tcggcagcac gtactgcatg aagatttgct tcgcggtcag
     7381 ctcctgttcc gctttttcgt tatagtcgtc cggataatca tttttgtact cttcgatcgg
     7441 cggcaagcca caggattgcg gggtatcgcg catcatcgca aaggcgaata atgccaccag
     7501 aatggcgcag aaagcaggca tatagagcgc cgcatgccag tcattgaacc aggccatccc
     7561 cagcaggaac agcagcggcg gaataccacc accgacgttg tgcgcacagt tccacactga
     7621 cacaatgccg ccacgttctt tctgcgacca ccagtgcacc atagtacgac cacacggcgg
     7681 ccaccccatc ccctggaacc aaccgcagag gaacaacagt acaaacatca ccgcaatgct
     7741 cgacgtcgcc catggcacaa agcccataaa caacatcact gccgccgcca gaatcaaacc
     7801 tgcgggcagg aaaacgcgcg gattcgagcg atccgatacc gaacccatga tgaatttcga
     7861 aaatccataa gcaatcgaga tccccgaaag ggcaaaacct aaatcaccgc gtgagaatcc
     7921 ctgctcaacc agataaggca tagcaagcgc aaagttctta cgaaccaaat agtaagccgc
     7981 atagccaaag aatatcccca ggaaaatttg ccagcgcaat cgacgataag tcggatcgat
     8041 ctccgcggca ggtaagcgcg ctttgtgtgg cgctggttta aaaatactca acattgatag
     8101 cctccgtggc ccgtggtctt atttatgatt aacagcctga ttcagtgaga gaacctgccg
     8161 tttcttgagt tgccgcgatg ttaagaaaac attcataaat taaatgtgaa ttgccgcaca
     8221 cattattaaa taagatttac aaaatgttca aaatgacgca tgaaatcacg ttttactttc
     8281 gaatcatgag cgaatatgcg cgaaatcaaa caattcatgt ttttactatg gctaaatggt
     8341 aaaaaacgaa cttcagaggg ataacaatga aaactcgcga ctcgcaatca agtgacgtga
     8401 ttatcattgg cggcggcgca acgggagccg ggattgcccg cgactgtgcc ctgcgcgggc
     8461 tgcgcgtgat tttggttgag cgccacgaca tcgcaaccgg tgccaccggg cgtaaccacg
     8521 gcctgctgca cagcggtgcg cgctatgcag taaccgatgc ggaatcggcc cgcgaatgca
     8581 ttagtgaaaa ccagatcctg aaacgcattg cacgtcactg cgttgaacca accaacggcc
     8641 tgtttatcac cctgccggaa gatgacctct cattccaggc cacttttatt cgcgcctgcg
     8701 aagaagcagg gatcagcgca gaagctatag acccgcagca agcgcgcatt atcgaacctg
     8761 ccgttaaccc ggcactgatt ggcgcggtga aagttccgga tggcaccgtt gatccatttc
     8821 gtctgaccgc agcaaacatg ctggatgcca aagaacacgg tgccgttatc cttaccgctc
     8881 atgaagtcac ggggctgatt cgtgaaggcg cgacggtgtg cggtgttcgt gtacgtaacc
     8941 atctcaccgg cgaaactcag gcccttcatg cacctgtcgt ggttaatgcc gctgggatct
     9001 gggggcaaca cattgccgaa tatgccgatc tgcgcattcg catgttcccg gcgaaaggat
     9061 cgctgctgat catggatcac cgcattaacc agcatgtgat caaccgctgc cgtaaacctt
     9121 ccgacgccga tattctggtg cctggcgata ccatttcgct gattggtacc acctctttac
     9181 gtattgatta caacgagatt gacgataatc gagtgacggc agaagaggtt gatattctgc
     9241 tgcgtgaagg ggaaaaactg gcccccgtga tggcgaaaac gcgcattttg cgggcctatt
     9301 ctggcgtgcg cccgctggtt gccagcgatg acgacccgag cggacgtaac gtcagccgtg
     9361 gcatcgtgct gctcgaccat gctgaacgcg atggtctgga cggatttatc accatcaccg
     9421 gtggcaaact gatgacctat cggctgatgg ctgaatgggc taccgacgcg gtatgccgca
     9481 aactgggcaa cacgcgcccc tgtacgactg ccgatctggc actgcctggt tcacaagaac
     9541 ccgctgaagt taccttgcgt aaagtcatct ccctgcctgc cccgctgcgc ggttctgcgg
     9601 tttatcgtca tggcgatcgc acgcctgcct ggctgagcga aggccgtcta caccgtagcc
     9661 tggtatgtga gtgcgaagcg gtaactgcgg gtgaagtgca gtacgcggta gaaaatttaa
     9721 acgttaatag cctgctggat ttacgccgtc gtacccgtgt ggggatgggc acctgccagg
     9781 gcgaactctg cgcctgccgc gctgccggac tgctgcaacg gtttaacgtc acgacgtccg
     9841 cgcaatctat cgagcaactt tccaccttcc ttaacgaacg ctggaaaggc gtgcaaccca
     9901 tcgcctgggg agacgcactg cgcgaaagcg aatttacccg ctgggtttat cagggactgt
     9961 gtggtctgga gaaggagcag aaagatgcgc tttgatactg tcattatggg cggcggcctc
    10021 gccggattac tctgtggcct gcaactgcaa aaacacggcc tgcgctgtgc cattgtcacg
    10081 cgtggtcaaa gcgcgttgca tttctcatcc ggttcgctgg atttgctgag ccatctgcca
    10141 gatggtcaac ccgtggcaga cattcacagt ggactggaat ctttgcgtca gcaggcacct
    10201 gcacatcctt actctcttct cgggccacaa cgcgtgctcg atctcgcttg ccaggcgcag
    10261 gcattaatcg ctgaaagcgg tgcgcaattg cagggcagcg tagaactggc tcaccagcgg
    10321 attacgccgc tcggcacttt gcgtgcaacc tggttaagtt cgccagaagt tcccgtctgg
    10381 ccgctacccg cgaagaagat atgtgtagtg gggataagcg ggctgataga tttccaggcg
    10441 catcttgcgg cggcttcgtt gcgcgaactg gatctttcgg ttgaaaccgc agaaatagag
    10501 ctgccggaac tggatgtcct gcgcaataac gccaccgaat ttcgcgcggt gaatatcgcc
    10561 cgtttccttg ataatgaaga aaactggccg ctgttacttg atgcgcttat tcctgtcgcc
    10621 aatacctgcg aaatgatcct gatgcccgcc tgcttcggcc tggccgatga caaactgtgg
    10681 cgttggttga atgaaaaact accttgttca ctgatgcttt tgccaacgct gccgccttcc
    10741 gtgctgggca ttcgtctgca aaaccagtta cagcgccagt ttgtgcgcca aggtggagtg
    10801 tggatgccgg gcgatgaagt gaaaaaagtg acctgtaaaa atggcgtagt gaatgaaatc
    10861 tggacccgca atcacgccga tattccgcta cgtccacgtt tcgcggttct cgccagcggc
    10921 agtttcttta gtggcggact ggtagcggaa cgtaacggca ttcgcgagcc gattctcggc
    10981 cttgatgtgc tacaaaccgc cacgcggggt gaatggtata agggcgattt ttttgcgccg
    11041 caaccgtggc agcagttcgg tgtaaccact gatgagacgc tacgcccgtc gcaggcaggg
    11101 caaaccattg agaacctgtt tgccatcggt tcggtgctgg gcggatttga tcccatcgcc
    11161 cagggatgcg gcggcggtgt ttgtgccgtc agtgctttac atgccgctca acagattgcc
    11221 caacgcgcag gaggccaaca atgaacgaca ccagcttcga aaactgcatt aagtgcaccg
    11281 tctgcaccac cgcctgcccg gtgagccggg tgaatcccgg ttatccaggg ccaaaacaag
    11341 ccgggccgga tggcgagcgt ctgcgtttga aagatggcgc actgtatgac gaggcgctga
    11401 aatattgcat caactgcaaa cgttgtgaag tcgcctgccc gtccgatgtg aagattggcg
    11461 atattatcca gcgcgcgcgg gcgaaatatg acaccacgcg cccgtcgctg cgtaattttg
    11521 tgttgagtca taccgacctg atgggtagcg tttccacgcc gttcgcacca atcgtcaaca
    11581 ccgctacctc gctgaaaccg gtgcggcagc tgcttgatgc ggcgttaaaa atcgatcatc
    11641 gtcgcacgct accgaaatac tccttcggca cgttccgtcg ctggtatcgc agcatagcgg
    11701 ctcagcaagc acaatataaa gaccaggtcg ctttctttca cggctgcttc gttaactaca
    11761 accatccgca gttaggtaaa gatttaatta aagtgctcaa cgcaatgggt accggtgtac
    11821 aactgctcag caaagaaaaa tgctgcggcg taccgctaat cgccaacggc tttaccgata
    11881 aagcacgcaa acaggcaatt acgaatgtag agtcgatccg cgaagctgtg ggagtaaaag
    11941 gcattccggt gattgccacc tcctcaacct gtacatttgc cctgcgcgac gaatacccgg
    12001 aagtgctgaa tgtcgacaac aaaggcttgc gcgatcatat cgaactggca acccgctggc
    12061 tgtggcgcaa gctggacgaa ggcaaaacgt taccgctgaa accgctgccg ctgaaagtgg
    12121 tttatcacac tccgtgccat atggaaaaaa tgggctggac gctctacacc ctggagctgt
    12181 tgcgtaacat cccggggctt gagttaacgg tgctggattc ccagtgctgc ggtattgcgg
    12241 gtacttacgg tttcaaaaaa gagaactacc ccacctcaca agccatcggc gcaccactgt
    12301 tccgccagat agaagaaagc ggcgcagatc tggtggtcac cgactgcgaa acctgtaaat
    12361 ggcagattga gatgtccaca agtcttcgct gcgaacatcc gattacgcta ctggcccagg
    12421 cgctggctta aactcccttc tgatgcccgg taagcatgtg gttaccgggc atttttgcgt
    12481 acacgattcc gtgcccaatg tatgcgttgc aacgcagtga aaattcctct gaaaacgtct
    12541 cgcaaaggct gaaactggca gatgtcaaag gcctgggata accgtaatgt cgcgtcatca
    12601 taaatatcag gtgacggaca accatgaccg aatcaacaac ctcctccccg catgatgcgg
    12661 tatttaaaac ctttatattc acacccgaaa ccgcacggga ttttctcgaa atacatttac
    12721 cagaaccact gcgcaagctt tgcaacctgc aaaccttacg cctggaaccc actagtttta
    12781 ttgaaaaaag tttacgcgct tactactcgg atgttttgtg gtccgtggaa accagcgacg
    12841 gtgacggcta tatctactgc gtgattgaac atcaaagctc tgcagaaaag aatatggctt
    12901 ttcggccaat gcgctatgcc actgccgcca tgcagagtca cctagataaa ggctatgaca
    12961 gagttccgct ggtggtgcca ttgctgtttt atcatggcga aacatcgccc tacccgtact
    13021 cacttaactg gctggatgag tttgacgatc cgcaacttgc ccggcagttg tacaccgaag
    13081 cttttccgtt ggtggatatc accatcgtac ctgacgatga gatcatgcaa catcggcgta
    13141 tagctctgct ggaactgatt caaaagcata ttcgcgacca cgatttaatc ggcatggtcg
    13201 acaggatcac cacgcttttg gttagaggct tcactaatga cagccagcta caaacactgt
    13261 ttaattatct gctgcaatgc ggcgatacct cccgtttcac ccgttttatt caggagattg
    13321 ccgaacgttc accactacaa aaggagagat taatgactat tgctgaacgg ctacggcagg
    13381 aaggacatca aattggctgg caagaaggta aattagtagg tttgcagcaa ggtaaattag
    13441 aaggtttgca ggaaggtatg catgaacaag ccattaaaat tgctttgcgc atgctggaac
    13501 agggcattga tcgtgaccaa gtgctcgcgg ccacccagct aagcgaagcc gatctggcag
    13561 cgaataacca ctaattaaca caggcccaca gccgatcccc atgggccttt gatatcaata
    13621 actacctttt atgcgtggcc catttttgcc tgatttaaac atcgccagtc gttgatccag
    13681 ggcatcgctg tagagcatcg tgtcaacgcc aacagcgaca aagttcgctc cccacgccag
    13741 gcattgctgc gccatatcag gagccacagc cagaaaacca gccgctttac ccgcagcacg
    13801 gatccgccga atgctggttt caataattcg ctgcacttcc gggtgcccgg cgttatccgg
    13861 gtagcccaac gacgcagaaa gatccgctgg tccaataaac acgccatcaa tcccttcgac
    13921 gtcgaggatc tcgtccaggt tatccagtgc cgttttactt tccacctgca ctaacagaca
    13981 aagcgaatcg ttgacttgcg ccatgtaatt ctcaatgcgt ccccagcgcg cagcccgtgc
    14041 cacactggcc ccaacaccac gctcaccgta gggagggttg gacaaatttt gccggagggg
    14101 gaatgtgccg gtgcagtaaa ccggcacaga gtggcgaaat cagacggata aagtcatgga
    14161 ctcaacaacc tcaatccagc catgctcact agcgatgtcc tggccgttta accaacggcg
    14221 tagcatattc agtgccatca ttgcgcacac ttcctgacgg atagccaggc tgtagcgtgt
    14281 agtgctgaaa cgcacacgca gagcgaaagt gccgtctggc gtggctagcg caaagttgag
    14341 atgctcgttc tcgaaacccg aaacagccag tgccagcccg gcaaaatggt tggcccgccg
    14401 ttctgtaatc cagtgcgcag tttgcgccag ggtttcttcc tgtgaaggaa tcacttcaca
    14461 cgccagcaat ggagcacctg cgcgagaaag ttgcaaagcc aataaaccac cggtgaattg
    14521 ctcgctcaac gtcaggctga actggcggtt ttgcaattcg cgactgatct gcgcgggcag
    14581 tccttcggtg ccttcgaaaa tcacgctctg tccggcaaca cgtttaacgt ccagccacag
    14641 tttttccatc gcctgttgct cgcttgccgg tccggtgagt ttcagttcga tgatcggcat
    14701 tgaggagcga tagcccattg ttacgcccgg cggcagttgt agagtgtcca ggctttgtgc
    14761 cagatcgctt tccgaacgac caaaggtagt taaacgcaga caaaccggcg gctgtggtaa
    14821 agaaaaacgc tcgcgcaggc gcggcaggat ttcgtgctcg accatcacct taaattctga
    14881 cggtacgccg ggagtaaaga acatcaggca acgattaagc tgcacggcaa aaccacaggc
    14941 ggtgcctacc gggttattga taaattcagc actggcaggc agctccgctt gtttacggtt
    15001 gctcggtgcc attactcgtc cacgttcgtg gaaatagcgt tccatctctt tgagccaggc
    15061 ttcatgcagc accaggcctt caccttttgc tgtcgcagcg gcgagtgcgc ttaaatcatc
    15121 gctggtcggt cccagcccgc cgttaacgat cagcacatcg gcgtgctgac tacgttcgcg
    15181 aagaatggtg actaagtcat caaggttatc ccccaccgta ttgcggcgag ataatggcag
    15241 cccctgatga aagaaaaaat cggccagcca ggcagcgtta gtgtcaacga tttgcccgta
    15301 taacacttca tccccggtgg atagcatttc cacttttaac atggttatct cctgcttttg
    15361 ttatcgaatc actataacgc aaagggagat aaggcgtagt aagaaactga atgccgcgcc
    15421 ggaagggatc agaaactggc gttaacccca acatacaggc cgtcagccac agcgttgtcg
    15481 cggttaccgt ctttacccga cagattcagg tagcgataac ccgcctcaat actgactgga
    15541 cgcataatgg tgtaacgcgc gccagcattc gcttcctcat aactttgaat accgctggag
    15601 agcgaatccg gagagtagta atactcgcca aacaaacgga agctgttgcc aattttccac
    15661 tgcaaaccac ctcctaccgc tgcggcataa ccttcatcgc cgtaattcgg gttggtgtac
    15721 acgccttttc cgccaacggt cgccattaac ggcccgagag gcagattcaa ccccagcccc
    15781 acgcccgcga cgtcaccgtc gtcgtcgtta tgtgtccagt taccgcttaa agccaggccc
    15841 gtcgattcag tcccaaaacc gacaccaata ttggtgtatt ctttacccgc ctggccgctg
    15901 atgctcattg cattgaccga tgcagaaacc aacagcattc cggccagacc tgctagcgca
    15961 atttttttca ttatcccctt tcccacaagc aattaatcaa aattcccaaa gctgcgggat
    16021 tgtactgcct ggcacagagg attcaatgag cgaaaaaagg gatctagtca cttattgtaa
    16081 ctaaatactt cttaaataat taaacgccga aaaaataaac acactttacc gatacacaca
    16141 aaaataataa taagtgcagt tatcattgca aaagagatta ctgtcacttt cacatatttc
    16201 tgagttaccg tattctcatg gtttcttttt atttataagg aatacccctg tgcgacaacg
    16261 gactattgta tgccctttga ttcaaaatga tggtgcttat ttgctgtgca aaatggccga
    16321 cgatcgcggc gttttccccg gtcaatgggc gctttcgggt ggcggcgtgg agtctggcga
    16381 acgaattgaa gaggcactac gccgcgaaat tcgcgaagaa ctgggagaac agctgctttt
    16441 gacagaaatc acgccgtgga ccttcagcga tgatattcgc accaagacat atgcagatgg
    16501 tcgcaaggaa gagatttata tgatttacct gatttttgac tgcgtttctg ccaaccgaga
    16561 agtgaaaata aacgaagagt ttcaggacta cgcgtgggta aaacctgaag atctggtgca
    16621 ttatgattta aatgttgcca cccgaaaaac gttacgtttg aaaggtcttc tttaacaatg
    16681 atgacagcca tcactggctg tcagattgaa aatttagtgg ttaacgaatt ccccatccag
    16741 ataaactttg cctttttcca catgcatgac taaaccatcc agataatcag gtttaaatat
    16801 cgcatcacgc ttatttttag caatatatgt caggcaatga ttatgggtga aaataacgat
    16861 atttttatca ggcgctttgc tttgtaagtc cttaattgca ctataaatct cattaccgca
    16921 ctgaagaagt cgtttatcta ccgtcagctt tttacccgcg gaaaaccagg tcgccgactg
    16981 aatggtccgg acggtattac tggaataaag atcgaaatca gggatgtcag cgctaaaagc
    17041 gttgcccagt tcacgggcat cctgggtacc tttaaccgta atacctgttt tatctgacaa
    17101 gcattgattg gttgaacgat cgcaacgttc agcatggcga aacaaaacga caaccgggtg
    17161 ctgctgtgcc agtctggcca atgttttatt gtcgatacgt ggcaaaccat tgctactcca
    17221 ggcggcatga gtacccagtc cggcaattgc agcgagcgcc agtaaaatga tgaaatattt
    17281 ttttgacttc aatgatgagc ggcaaaaagc taacacgtaa actccaccta tagacaagcg
    17341 caaccagaca attaccgtga aattgagcga catttctggc gataattagc agttggtgta
    17401 atattaaaaa tcctacgatg tcggcaaaat gcctcaaatt tttgccaaat gcaaagccta
    17461 aataagaaaa aatataaaaa tttcaatatt tacgtctaat attagtttct taaggttaag
    17521 ttaatattct atccttaaaa tttcgctcca aatggcaaaa tatacacaac actctttata
    17581 gcaaatataa gtggacaggt attcaatggc ggaaggaaaa gcaatgtcag aatttttgcc
    17641 tttttcgcga ccagcaatgg gcgtggagga actcgctgca gttaaagagg ttctcgaatc
    17701 cggttggatc acaaccggtc cgaagaatca ggcgcttgag caagcctttt gccagttgac
    17761 gggaaatcag catgccatcg cggtcagttc agccaccgcc ggaatgcata tcacgctaat
    17821 ggcgttggaa attggcaagg gcgatgaagt gattacgcct tccctgacct gggtttcaac
    17881 cctcaatatg atttccttgc tgggtgcaac gccggtaatg gtggatgtcg accgcgatac
    17941 gctgatggtc acgcctgaag cgatcgaatc agccattacg ccacgcacta aagccatcat
    18001 tccggtgcat tatgccggtg cgccagcaga tattgacgcc attcgcgcca ttggcgaacg
    18061 ttacggcatc gcagttatcg aagatgctgc ccatgccgtc ggtacgtatt acaaagggcg
    18121 acatattggc gcaaaaggta ccgctatttt ttcatttcat gccattaaaa atattacctg
    18181 tgctgaaggt ggcctgattg taactgataa tgaaaacctt gcccgccagc tacggatgct
    18241 gaaatttcac ggtctgggtg tcgatgccta tgacagacaa acctggggcc gtgcaccgca
    18301 ggctgaagtc ttaacaccgg gctataagta caatctgacc gatattaacg ccgcgattgc
    18361 cctgacacag ttagtcaaat tagagcacct caacacccgt cggcgcgaaa ttgcccagca
    18421 atatcagcaa gcactggcag ctctcccctt tcagccatta agccttcccg cctggccgca
    18481 cgttcacgcc tggcatctgt ttattattcg tgtcgatgaa caacgttgtg gtatcagtcg
    18541 cgatgcgttg atggaagcgt taaaagaaag aggcattggt accgggttac atttccgcgc
    18601 cgctcacaca caaaaatatt atcgcgagcg ttttcccaca ctgtcgttac cgaataccga
    18661 atggaatagc gaacgcatct gttcgttgcc gctgttcccg gatatgacta ccgccgatgc
    18721 cgaccgcgtc atcacagccc ttcagcaact cgcaggacaa taagccatgt ttgaaatcca
    18781 ccctgttaag aaagtctcgg tggttattcc cgtttataac gagcaggaaa gcttaccgga
    18841 attaatcagg cgcaccacca cagcctgtga atcgttgggg aaagagtatg agatcctgct
    18901 gattgatgac ggcagtagcg ataattccgc gcatatgctg gtcgaagcct cacaagcgga
    18961 gaacagccat attgtgtcta ttttgattaa ccgcaattac gggcaacatt cagcgattat
    19021 ggcgggattc agtcacgtta ctggcgactt aattattacc cttgatgccg atctccagaa
    19081 tccgccagaa gaaatccccc gcctggtggc aaaagccgat gaaggttacg acgtggtagg
    19141 gactgtacgc cagaaccgcc aggacagctg gtttcgtaaa acagcttcga agatgattaa
    19201 ccggcttatt cagcgcacca ctggcaaagc gatgggtgac tacggttgta tgctgcgcgc
    19261 ctatcgccgt catattgtcg atgcgatgtt gcactgccat gaacgcagca cctttatccc
    19321 gattctggcg aatatcttcg cccgccgtgc aattgaaatt ccagtacatc atgccgagcg
    19381 tgagtttggt gaatccaaat acagttttat gcgcctgatt aatttgatgt acgacctggt
    19441 gacctgcctt accacaacgc cgctacgtat gctgagtctg ctcggcagca ttattgcgat
    19501 tggaggtttt agcattgcgg tgctgctggt gattttacgc ctgaccttcg gaccacaatg
    19561 ggcggcagaa ggcgtcttta tgctatttgc cgtgctgttt acttttattg gcgctcagtt
    19621 tatcggcatg ggattactcg gtgaatatat cggcaggatc tacaccgatg tccgcgcccg
    19681 cccccgctat tttgttcagc aagttatccg tccatccagc aaggaaaatg aataatgaaa
    19741 accgtcgttt ttgcctacca cgatatggga tgcctcggta ttgaagctct gctggctgcc
    19801 ggttacgaaa ttagcgccat ttttacccat accgataatc ccggtgaaaa agccttttat
    19861 ggttcggtgg ctcatctggc ggcggaaaga gacattccgg tttatgcgcc ggataacgtt
    19921 aatcatccgc tgtgggtgga acgcattgcc caactgtcac cagaggtgat tttctctttt
    19981 tattatcgcc atcttatttg cgacgagatt tttcagctcg ctcccgcagg tgcatttaat
    20041 ctgcacggtt cgctgttacc aaaatatcgt ggtcgcgcgc cgctgaactg ggtgctggtg
    20101 aacggtgaaa cggaaacggg cgttacattg caccgaatgg taaaacgtgc cgatgctggg
    20161 gccattgtgg cgcaactgcg cgttgccatt gcgccagacg atatcgctat tacgctgcat
    20221 cataaattgt gccatgccgc gcgccagcta ctggaacaga cattacccgc cattaaacac
    20281 ggtaatattc tggaaatcgc ccagcgcgaa aacgaagcca cctgttttgg tcgcagaacg
    20341 ccggatgaca gtttccttga atggcataaa ccggcatccg tactgcacaa catggtacgt
    20401 gccgttgccg atccgtggcc gggtgccttc agctatgttg gcaatcagaa attcaccgtc
    20461 tggtcgtcgc gtgttcatcc tcatgccagc aaagcacagc cggggagcgt gatttctgtt
    20521 gcgccactgc tgattgcctg tggcgatggc gcgctggaaa tcgtcaccgg acaggcgggc
    20581 gacggcatta ctatgcaggg ctcgcaatta gcgcagatgc tgggcctggt gcaaggttca
    20641 cgcttgaata gccagcctgc ctgcaccgcc cgacgccgta cccgggtact catcctcggg
    20701 gtgaatggct ttattggcaa ccatctgaca gaacgcctgc tgcgcgaaga tcattatgaa
    20761 gtttacggtc tggatattgg cagcgatgcg ataagccgtt ttctgaatca tccacatttt
    20821 cactttgtcg aaggcgatat cagtattcat tccgaatgga ttgagtatca cgtcaaaaaa
    20881 tgtgatgtcg tcttgccgct ggtggcgata gccacgccga ttgaatatac ccgcaacccg
    20941 ctgcgcgtat ttgaactcga ttttgaagag aatctgcgca ttatccgcta ctgcgttaag
    21001 taccgtaagc gaatcatctt cccgtcaact tcagaagttt atgggatgtg tagcgataaa
    21061 tacttcgatg aggaccattc taatttaatc gtcggcccgg tgaataaacc acgctggatt
    21121 tattcggtgt caaaacaatt acttgatcgg gtgatctggg cctatggcga aaaagagggt
    21181 ttacagttca ccctcttcct cccgtttaac tggatgggac cacgactgga taaccttaat
    21241 gcagcgcgaa ttggcagctc ccgcgctatt acgcaactca ttctcaatct ggtagaaggt
    21301 tcaccaatta agctaattga tggcggaaaa caaaaacgct gctttactga tatccgggac
    21361 ggtatcgagg cgttgtaccg cattatcgaa aatgcgggaa atcgctgcga cggcgaaatt
    21421 atcaacattg gcaatcctga gaacgaagcg agcattgagg aactgggcga gatgctgctg
    21481 gcgagcttcg aaaaacatcc gctgcgccat catttcccac cgtttgcggg ctttcgcgtt
    21541 gtcgaaagta gctgctacta cggcaaagga tatcaggacg tagagcatcg taaaccgagc
    21601 atccgcaatg cccaccgctg cctggactgg gagccgaaaa ttgatatgca ggaaaccatc
    21661 gacgaaacgc tggatttctt cctgcgcacc gttgatctta cggataaacc atcatgacca
    21721 aagtaggctt acgcattgat gtcgatacct ttcgtggcac ccgtgaaggc gtgccgcgtc
    21781 tgctggaaat cttgagtaag cataatattc aggccagcat ttttttcagc gtcggcccgg
    21841 acaatatggg ccgccatctc tggcgactgg tgaagccaca gtttttgtgg aagatgctgc
    21901 gctcaaacgc ggcatcgctt tatggctggg atattttact ggcaggtacg gcctggccag
    21961 gtaaagagat tggtcatgcc aatgccgata tcattcgtga agcggctaaa catcacgaag
    22021 tcggtctgca cgcctgggat caccatgcct ggcaagcccg tagcggtaac tgggatcggc
    22081 aaacaatgat cgacgatatt gcacgcggtc ttcgcactct ggaagagatt atcggtcaac
    22141 cggtaacctg ttctgccgct gcgggctggc gtgccgacca gaaggtgatc gaagcaaaag
    22201 aagcgttcca tttgcgctac aacagcgatt gtcgtggggc cataccgttc cgtccactgc
    22261 ttgaatcagg aaaccctggc actgcgcaaa ttccggtgac cttacccacc tgggatgaag
    22321 tgattggtcg ggatgtgaaa gcagaagatt ttaacggttg gttactcaac cgcatcctgc
    22381 gagataaagg cacgccggtt tataccattc atgcagaagt tgaaggctgc gcttatcagc
    22441 ataattttgt ggatctcctc aaacgcgcag ctcaggaagg cgtgacattt tgccctttaa
    22501 gcgaactgtt atcagagacg ttgccgctcg gacaagttgt tcgcggaaat attgccggac
    22561 gtgaaggctg gctgggttgc caacaaattg cgggtagtcg ctgatgaaat cggtacgtta
    22621 ccttatcggc atcttcgcgt ttattgcctg ctattacctg ttaccgatca gcacgcgtct
    22681 gctctggcaa cccgatgaaa cgcgttatgc ggaaatcagt cgagaaatgc tggcatccgg
    22741 cgactggatt gtgccccatc tgttagggct acgttatttc gaaaaaccca ttgccggata
    22801 ctggattaac agcattgggc aatggctatt tggcgcgaat aactttggtg tgcgggcagg
    22861 cgttatcttt gcgaccctgt taactgccgc gctggtgacc tggtttactc tgcgcttatg
    22921 gcgcgataaa cgtctggctc tactcgccac agtaatttat ctctcattgt ttattgtcta
    22981 tgccatcggc acttatgccg tgctcgatcc gtttattgca ttctggctgg tggcgggaat
    23041 gtgcagcttc tggctggcaa tgcaggcaca gacgtggaaa ggcaaaagcg caggattttt
    23101 actgctggga atcacctgcg gcatgggggt gatgaccaaa ggttttctcg cccttgccgt
    23161 gccggtatta agcgtgctgc catgggtagc gacgcaaaaa cgctggaaag atctctttat
    23221 ttacggctgg ctggcggtta tcagttgcgt actgacggtt ctcccctggg gactggcgat
    23281 agcgcagcgg gagcctgact tctggcacta ttttttctgg gttgagcata ttcaacgctt
    23341 tgcactagat gatgcccaac atagagctcc gttctggtac tacgtgccgg tcatcattgc
    23401 cggtagcctg ccgtggctgg gattactccc cggtgcactg tacacaggct ggaaaaaccg
    23461 caagcattcc gcaaccgtct atttgttgag ctggacgata atgccgctgc tgtttttctc
    23521 cgtcgctaaa ggcaaattgc ccacctatat tctttcttgc tttgcacctc tggcaatgct
    23581 gttggcgcat tacgcgttgc tggcagcaaa aaataatcct ctggcgctgc ggattaatgg
    23641 ctggattaac atcgcttttg gcgtcactgg cattattgcc acatttgtga tctccccctg
    23701 gggaccaatg aacacgccgg tgtggcaaac cttcgagagc tataaagtct tttgtgcctg
    23761 gtcgattttt tcgctatggg catttttcgg ctggtacacc ttaacaaacg tcgaaaagac
    23821 ctggtctttc gccgcgcttt gcccgctggg gctggcgttg ctggtaggat tttcaattcc
    23881 tgacagagtc atggaaggaa aacatccgca attttttgtc gagatgacac aagaatcact
    23941 tcagccaagc cgctatattc ttactgatag cgtcggtgtt gccgcaggtc tggcatggag
    24001 cctgcaacgc gatgacatca tcatgtatcg ccagacaggt gagttgaaat acggccttaa
    24061 ttatccggat gcgaaaggga gatttgtcag cggtgatgag ttcgcaaact ggcttaatca
    24121 acatcgtcag gaggggatta ttacactcgt gctttcggtt gaccgcgatg aagatatcaa
    24181 cagtctcgcc attccgcccg cagatgccat cgttcgtcag gagcgtctgg tgctgattca
    24241 gtatcgtccc aaatgatctg gctaacatta gtctttgcca gcttgcttag cgttgccggg
    24301 cagttgtgtc agaaacaggc aacctgcttt gtggcgataa acaaacggcg caaacatatc
    24361 gtgctgtggc tgggactggc gctggcttgt cttggtcttg ccatgatgct ctggctgctg
    24421 gtcttgcaga acgtaccggt aggcattgcc tacccgatgt taagtctgaa ttttgtctgg
    24481 gtgacgctgg ctgcagtaaa actgtggcac gaaccggtat cgccgcgtca ctggtgtggg
    24541 gtggcgttca ttattggtgg cattgtgatc ctcgggagta cggtgtaatg ggcctgatat
    24601 ggggattatt cagcgtcatt attgcctcag ttgcgcaatt aagcctgggt tttgcggcaa
    24661 gtcatctgcc gccgatgacg cacttatggg attttatcgc ggctctgtta gcttttggcc
    24721 tggatgcccg gatactgtta ctcgggctgc tgggttatct gctttccgtt ttttgttggt
    24781 ataaaacgct acataaactt gcgctaagca aagcctatgc cttgctcagt atgagttatg
    24841 tgctggtatg gattgcttca atggtacttc ccggctggga agggactttt tcgctgaaag
    24901 cactactggg agtagcctgt attatgagcg ggttgatgct gattttcctg cccacgacaa
    24961 aacaacgtta ctgagttttc cctgccgctg tacactgccg ttcccactca tcggggctat
    25021 aactgcttgc cgagaggact ttcaccgtat gcagcttttc acgattaata caataaagcg
    25081 cattctgcaa aggcgagagt aaatccccca ctttcacggc gaagtcactc ttcacttcgg
    25141 cgatcatttt tatcgcgccg ccagcatcgc agagcataag tacatgtctg ttatcttgtt
    25201 tattgcagca cgattttttg tgccagccat tccattgcat tatcctgttt gctaagagtt
    25261 ttcacatcaa tttacagatt aacagataaa ctctttatca acggcaatgg aatggcagaa
    25321 atgagaagtg aaacggaggg ttaattattg ctgacgttgc acccactctt ttagcgcctg
    25381 acgtgaaatt ttaataccgc cgtttttcag ctccggcggt aaagtcagcc agcgcaccgg
    25441 ttgttgaaaa cgagccagct tatctttcac ccattcgcca agatcaacgg tttgctggtc
    25501 atactccacc accgctaccg gccgatgacc aaactccttg tcggcaacgg aaacgataaa
    25561 cacctgcaaa accgcaggat gtgcagcaat aacacgctcg acttcttccg gctgaatacc
    25621 ctcaccgcca ctgaagaaca gattgtctaa acgtccgaca atggtcagct tgccattatg
    25681 catctcaccg cgatcgcgcg tagcgtacca gccttcgtca ttaaccagtg aaaccagttg
    25741 cccgttacgc cagtaacctt ctgccatact ggcagcccgc agccacactt cattattaac
    25801 gattttcact tcccgacccg gcagcggcaa accaacgtct gccaggccgt cggcttcttt
    25861 cgcacatacc gtggaggcaa actcggtcag accatagccg caaaagcaac gaatcccctg
    25921 ctcgcgcgcc tgttccgtca actcgaccgg gatagccgcg ccgccaagta acaccgcttt
    25981 cagggaaacg gaactgcggt taaccagcaa acgccagagt tgtgttggca ccagtgaagc
    26041 gtgagtacag cctgccagca tttgctccaa tggctgttta tcacgcaccg tcatccgcgc
    26101 accagcgtat aaccagcgcc acataattcc ctgaccggag acgtgaaaca gcggtaaaga
    26161 gagcaaccag tcatcgtgat cgccaaacgg aatcagcgat aacacacctt gcgcactggc
    26221 aagatgggcc tgataagtat ggacagcggc tttcggcaaa ccggtagaac ccgaggtcaa
    26281 cgtcattgag cacagacgcg tcggctgcca cgcagcggca tgtacgcctt caaccagctg
    26341 aatgtgcagc gacgttaatg ccggaaacgt gttttcccca tccggcacca gcgcaaattg
    26401 cagcgtcaga ttgggcagca attcttcaag caacggttgc ggcagctgag ggttcacggg
    26461 caacacccgc gccccgcatt gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt
    26521 ccacgcccgc aacatcacgc cgctgccctc aaccaccccc tgcgccgcaa agcccgtagc
    26581 taactcatcg acgcgagcac aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa
    26641 ggcgatggct tctccccgca cttgccgcca gtgacgccac ggccagtcag agaagatcat
    26701 aacaactgct cctgtacatc agcgtccact aacggcagtg ggctccccgg ccagcgacga
    26761 acctgctgcg cctgcatcag atccagcgtg tccagtcccg gaatggtgtc tggcgttaac
    26821 caggcagcaa tccgcgccag ttgcgttaga cctaagctcg actcaatgga agaactgatc
    26881 accgccgtca gccccagcgc atgcgccgcc tgtacctgct cgcgtacttt ttccagactg
    26941 cccgtgagcg tgggtttgat aactaccgcg cgcacgccct cttcagccac aaaggcaaaa
    27001 tccggctcgc gcaggctttc atcccaggca atggcaatgc cggtttcacg ggcaaacgct
    27061 cgcgaatcat cgcgggtttt gcacggctct tcgagaaacg cgatgcggtg gcgataatcc
    27121 gggttaacat atttggcaaa ctgctgacct ttcagcggtg tccaggcgcg atttgcgtca
    27181 agacgcaaat gcagatccgg aattgcctcc agcaacagat tcaccaccat gccgtcgcgc
    27241 accgcttcgt acaatcccac tttgaccttc gccactttct cgcccggcat atcggcaagt
    27301 ttgaggatca gatcgtccgg atcgccatta cacagcggtg ccgcacggta gttggctgct
    27361 tgcggcaacg tatctgtcag ttctgccaat gcacagctta cgccaaaggc cacggaaggc
    27421 atctgcggta gctcgcaatc gcctgccaac cagttattta cccaggcaag cagcacgctt
    27481 tgcgcctctt cccagttttc ctggctgaag cccggcagtg gggagatctc cccccaccct
    27541 tcgcgctcgc cctcacgcag gcaaacgtac agcccgtcgc gggtttttaa ccgcctgtcg
    27601 cgcagaacca cccccgcgtc catggggatc tgccagcggt atacctgcgc gctacgcatt
    27661 acggattccg tttaaatttg ctgaaatcag gctgacgttt ctggttgaaa gcgttgcgac
    27721 cttcctgacc ttcttccgtc atgtagaaca gcatggtggc gttgcccgcc agctcctgca
    27781 gccccgcctg cccgtcacag tcggcgttca gcgcagcttt caggcagcgc agcgccatcg
    27841 ggctgttttg cagcatttcg cggcaccaac ggacggtttc tttttccaga tccgccagcg
    27901 gtaccacggt gttgacaagg cccatatcca gcgcctgttt tgcgtcgtac tgacggcaca
    27961 ggaaccagat ttctcgcgct tttttctgcc cgacgatacg agccatgtag gaagcgcccc
    28021 agccgccgtc gaaggaaccg actttcgggc cagtctgacc gaagatggca ttatctgctg
    28081 cgatagtcag gtcgcacatc atgtgcagaa cgtgaccacc gccgatggag tagccagcca
    28141 ccatcgcgac aaccggtttc ggacaggtac ggatctgacg ctggaagtcc agcacgttca
    28201 ggtgatgtac gccggaatca tctttatagc cgccgtaatc accacgcact ttctggtcac
    28261 caccggagca gaacgcttta tcgcctgcgc cagtcagaat gatcacgccg atattgtcgt
    28321 cataacgcgc atctgccagt gcctggatca tctcttttac cgtcagagga cggaaggcat
    28381 tgcgcacctg cggacgatta atggtgattt ttgcgatacc gtcggtggat ttttcataac
    28441 gaatgtcctc gaaaccttcg gagcagtcgt gccattcaac cggtgcgtaa ggcattgctt
    28501 catcaggata aatcatattg tgtcctttag tcagaaacgc aagatctgcg ccagacttgc
    28561 gattacgcca gcgggatttt cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca
    28621 gtcggcagcc agttccgccg ccagggcgcg gaatttgctg tcacgttcac cacataaata
    28681 ataaaacgca aatgtgcggg cgctaaggtt agcacgtaaa tcaggctgga cggcgagaga
    28741 agtcgcctcc agcatggcgg caagggttgc gccattattg ttgctgcgca gcgccaccag
    28801 ctcccggcgt tgatcgtcat tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa
    28861 taccgccgtt aacggttctg tgcgaaaacg ctgcgcccat tggcgatcgg aacgctgacg
    28921 ttccgcacgt tgttcagcat tttgcagccc cggatgcccg ccttcgacaa taaccccaca
    28981 aagccccgcc agcccctggc aagccgccat catcgccacc cgtccgccaa gcgagtaccc
    29041 caccagccag aagtcaagga tgttgtaact aaccaaggtt ttacgcagta agtcggtgac
    29101 atcatcaaat ccatcgacgc taatcgccgc cgaaccacca tgacctagga gatcaacata
    29161 caaccgtgag tagtcggcaa acgcctcgcc cacttcttgc cattcgtggc aatcgccgga
    29221 aaaaccgtgc aaaaacacca gccagggtaa acctagtttt ccgtgttttg cctgcgcgtg
    29281 caggatcata aatggcttac ctgcgccaga agttgctgga gcgtttgcgc accatcggtg
    29341 tcgttaacca ccatttcaat caccgtggtg gttggcgtgc gccaggcgtc ggcaaatgcc
    29401 gtttcaagtt cctgccagtt ttgcggatga tgatatttca gctcgaacat cgcggcggcg
    29461 tgctcaaaat ggacgttttg cggcatcaga tagaaacgct cacgctcgct ttgcggcgtt
    29521 ggcaacagcg agaaaatttg cccgccgttg ttgttcacca caattaatac cagcggcgca
    29581 gaaacctgac gcaataacgc cagcgcgttg agatcgtaaa gcgcggagag atcgcccaca
    29641 atcgccagcg tcggtttgcc gcttgcccgc tgaacgcctg cggcggtcga aagcagtccg
    29701 tcgataccgc tggcccctcg gttgctgtac accgggtaac ctgccggaag ttgcgaaagc
    29761 gcatcaatca gacgtaccac caggctgtta ccaacaaaca attgcccctg ttcaggcaga
    29821 tagtcgctga tgcgatgcgc cagttgcgct tcgccaaacg catcacggcg ggcaataacc
    29881 gcctgcattg cctgttccgc caggcgcggg atttcaacgc accagggctg gcgtttttct
    29941 gccggatgca gctccagcca gtcggcaata ttggcaatta agcgacgtcc gcgatggtgt
    30001 gccggatcaa gtcgcccttc aatgtcatca acaatccagt actcttctgg ttcacagctt
    30061 gcctgccatt gcaggagccg tttccccgtc aggctacttc ccagttgcac cacaatttgc
    30121 gcctgctgca gctcgctggt cgctttggca ttgcctaacc aaagatcggc acacggcagc
    30181 ggctgcccgg tttgtgacag cacatcgcca atcagcggcc agccaagagt ttgcgcccac
    30241 agggcaactt ttttgccctc ttccgcactc atgcgcccgg caaccaccac gccgcgcttt
    30301 tgtcgccaga agaaccagtc gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc
    30361 cacggtttgt cgtcctgcca ccagtcaccc agacgctgtt gccagctaag cccggtatcg
    30421 tccatttcgc catacagcgg ttcagcaaac gggcagttga tatggactcc ccccgcatga
    30481 agcgtaccga gagcgtggtc gatggtagaa accagccaac gtgcggggat atcctgggtc
    30541 gggcgcggca atgaaatact gtgcgtgggg tgagaggcga acattcccgg ctggcgaatt
    30601 gcctgattcg cgccgcagtc aattagctcc ggcgggcgat cggcggttaa gagaatcagt
    30661 ttttctccgg ttaacccggc ttcaatcagt gccggataga gatttgccac cgccgtgccg
    30721 gaggtcacaa tcaccgccac cggctgcttg ctgactttcg ccagccccag cgccagatgc
    30781 cccaacccac gctcatcgaa atgggtgtgg tgaatgaatg cggaattctc cgccgccgct
    30841 aacgttaacg gtgtagaacg cgagcctggg gcgatacaga tgtgtctgac gccgtgacgc
    30901 gttaatgctt ccagaatgac cgccgcccag cgtcggttaa atgcgcttac tgacatgagt
    30961 ttgtccggta tcaatattgc ggctaagtat aaggagctac aaaaatagat tattgatatg
    31021 aatcggtaat gatgcgactc attactattc catttgtaat aaagtacgca gccctgccgc
    31081 tttgttgtcg atttcctgcc actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc
    31141 agcatataat cgcacgacat tgccgctaat ttttgctgag cgcagggaaa cgcagaattc
    31201 gctttgttgt aatgagagat agcccgcaga accggcgtac cattctcggg tgaacggttc
    31261 gtgacgggcg ataaactgtc gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg
    31321 ctgcaactga tgtaaacaga tcacatcatc cgctttgttg agtgaagtcc agatacagcg
    31381 gcgaagatgc tgcactttac gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg
    31441 ggtatcggcc tgtaatcgtt ggcagatatc ttccaccacc agcatgttct cgcgctggtt
    31501 tttatcatcc gccatcagcc actctcctaa ctgctgcgcc tgcttatcat caggatgatt
    31561 tgctactgtt cccgccagcg cttcagtacg cagcgctttg tcacgccgcc gccataaccg
    31621 ttccggtgaa gagccaagaa aagcattttc gccatcaaag gccatgtaaa aatggtagca
    31681 attcagattc agtcgacgac tggcagccat catcgccgcc gcgttgaccg gacttgcgaa
    31741 atgcaggtca gttgcccgag cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt
    31801 cgttgccagt tcgattaatt gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt
    31861 ggtggttaaa tgtaacccag gcaagggctt gatactcacc agtgtggcga taaattcttt
    31921 tgcctgaatc gcatcgtgct gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc
    31981 tttaccgcca cagcgtcgcc attccaggcg gggtaaaagt aaattgccct gcgacgggtc
    32041 aaaagcattc agcccccaaa tgcgtaagtc agcgtgttcc gggtgctggc gaagaaaacg
    32101 ttgtgcctgg tccaacgacg taaaacgggt aatcgcgccc aggacggcag cttcttcatc
    32161 accattacgt tgttgccagt agaattgcgg gtacgtttgc tgactggcca gccagctcaa
    32221 ggcatcaaaa gcgtctttga gagggaaagg aatatcgata acccgaatgc cgggtgtcgc
    32281 cggaatctct tgcgacaaat ggcgcagtag attttccagc gccgtagtaa gtgattgcac
    32341 gcggacctct ccctgttaaa agcctcacat tatacggggt actacaaaaa aaatgcagta
    32401 ccccggtgta gggaggtttt aacggcgtgc cagcaacagt cctagtacca gcccgacggc
    32461 cgcgcccaca ccaattcctt gccagggttt ttcgtggacg tagtcatcag cacgataaac
    32521 agcctgcttc gcccgatagt aataactgtc tgaagcctgg ctaacccgtt ttttcacatc
    32581 atccagcgct ttttctgcat gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc
    32641 agaggagcgg agcacctctt ccagtgtttc actaagcagc gtcaggtcgt catcgatacg
    32701 tgtatcacca aactgattag acattctcgt tctccatttg cgtaaaacct gtcactaact
    32761 atagacaaga gttgttgatt acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat
    32821 accatcttct tcatagacct ccgtcacagg gataaaaccg aaactctggt agaagttttg
    32881 cagatgcgcc tgcgccccga ggtaaacagg cttatcaggc cagtgatgcg tacatgtttc
    32941 gagtgtttta ctcatcagct gctgccccac tttttcacca cgcaacgctt cactgacaat
    33001 cacccgacct ataacgaccg gctcaagatc atcatcactt ttcagaatcc tcgcatacgc
    33061 caccagttca tcatttttcc aaccgagaat atggcgatta tcccccgtca aatcatcacc
    33121 atcgatatcc tgataagggc aattctgctc gacaacaaac accgcgcaac gcagttgtaa
    33181 taaggcataa agctgggaaa ccgataattc agagtgatgc agatcttgcc attcaatcat
    33241 aatgctctcc ttttagatgt atgccttata ctagccggaa tattttttga aacgtgatga
    33301 actcatggaa ttaatttttt taggtacttc agccggtgtg ccaacccgca cgcgcaatgt
    33361 cacggcaata ttgcttaacc tgcaacatcc gacccagagc ggactttggt tgtttgactg
    33421 cggtgaaggc acccagcatc agctactgca taccgccttt aaccctggaa aactggacaa
    33481 gatttttatc agtcaccttc atggcgatca tctttttggt ttacccggct tgctgtgcag
    33541 tcgttctatg tcaggcatta tccaaccctt aacgatttat ggtccgcaag gtatccgtga
    33601 atttgtggaa accgcgctgc ggattagcgg ctcatggacc gattatccgc tggaaattgt
    33661 cgaaattggc gctggcgaaa ttctcgatga tggcctgcgc aaagtaaccg cttatccgct
    33721 ggaacaccca ctggaatgtt atggctatcg tattgaagaa catgataaac cgggtgcatt
    33781 aaatgcccag gcattaaaag ctgctggcgt gccgcctggc ccactgtttc aggaattaaa
    33841 agcgggcaaa acaatcacgc tggaagatgg aaggcagatt aacggcgcag attacttagc
    33901 tgctccagtg ccaggtaaag cgctcgctat tttcggcgat accggcccct gcgatgccgc
    33961 acttgacctg gctaaaggtg tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat
    34021 ggaagccaaa gccaatagtc gcggccatag ctctacacgc caggctgcga cactagcccg
    34081 tgaggctgga gtcggcaagc taatcattac ccacgtcagc tcgcgctatg atgacaaagg
    34141 ttgtcaacac ctgttacgtg aatgcaggtc aattttcccg gcgactgaac tggcgaatga
    34201 tttcaccgtg tttaacgttt aactctgctt attattaaaa acagggtgaa acttgccctg
    34261 ttatagcaac ccgc
//
LOCUS       DAJVPP010000037        33700 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVPP010000037 DAJVPP010000000
VERSION     DAJVPP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.037"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..152
                     /locus_tag="OQM34_001742"
     CDS             <1..152
                     /locus_tag="OQM34_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1452227.1"
                     /translation="WLDLVAIFRKREYLPWRVQYVLDEMLREIRHQLAQSQQLRPEQA
                     AESED"
     gene            complement(149..1285)
                     /locus_tag="OQM34_001743"
     CDS             complement(149..1285)
                     /locus_tag="OQM34_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1452228.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNPE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1291..1614)
                     /locus_tag="OQM34_001744"
     CDS             complement(1291..1614)
                     /locus_tag="OQM34_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1452229.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            1785..1940
                     /locus_tag="OQM34_001745"
     CDS             1785..1940
                     /locus_tag="OQM34_001745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452230.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            complement(2159..3697)
                     /gene="aldB"
                     /locus_tag="OQM34_001746"
     CDS             complement(2159..3697)
                     /gene="aldB"
                     /locus_tag="OQM34_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCS1452231.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3862..4911)
                     /gene="yiaY"
                     /locus_tag="OQM34_001747"
     CDS             complement(3862..4911)
                     /gene="yiaY"
                     /locus_tag="OQM34_001747"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCS1452232.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVKEEDFAVLATNALKDACGFTNPI
                     QATHEEIVVIYRAAM"
     gene            complement(5101..6945)
                     /gene="selB"
                     /locus_tag="OQM34_001748"
     CDS             complement(5101..6945)
                     /gene="selB"
                     /locus_tag="OQM34_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1452233.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(6942..8333)
                     /gene="selA"
                     /locus_tag="OQM34_001749"
     CDS             complement(6942..8333)
                     /gene="selA"
                     /locus_tag="OQM34_001749"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1452234.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEAIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8431..9039)
                     /locus_tag="OQM34_001750"
     CDS             complement(8431..9039)
                     /locus_tag="OQM34_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1452235.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9110..10195)
                     /locus_tag="OQM34_001751"
     CDS             complement(9110..10195)
                     /locus_tag="OQM34_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1452236.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10198..10560)
                     /locus_tag="OQM34_001752"
     CDS             complement(10198..10560)
                     /locus_tag="OQM34_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1452237.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11097..13010
                     /gene="mtlA"
                     /locus_tag="OQM34_001753"
     CDS             11097..13010
                     /gene="mtlA"
                     /locus_tag="OQM34_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1452238.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHIRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13105..14253
                     /gene="mtlD"
                     /locus_tag="OQM34_001754"
     CDS             13105..14253
                     /gene="mtlD"
                     /locus_tag="OQM34_001754"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1452239.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14253..14840
                     /gene="mtlR"
                     /locus_tag="OQM34_001755"
     CDS             14253..14840
                     /gene="mtlR"
                     /locus_tag="OQM34_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1452240.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(14852..15061)
                     /locus_tag="OQM34_001756"
     CDS             complement(14852..15061)
                     /locus_tag="OQM34_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452241.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15345..15707
                     /locus_tag="OQM34_001757"
     CDS             15345..15707
                     /locus_tag="OQM34_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1452242.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16251..16934
                     /locus_tag="OQM34_001758"
     CDS             16251..16934
                     /locus_tag="OQM34_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1452243.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            16978..21640
                     /locus_tag="OQM34_001759"
                     /pseudo
     CDS             16978..21640
                     /locus_tag="OQM34_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22008..23663
                     /gene="lldP"
                     /locus_tag="OQM34_001760"
     CDS             22008..23663
                     /gene="lldP"
                     /locus_tag="OQM34_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1452244.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23663..24439
                     /gene="lldR"
                     /locus_tag="OQM34_001761"
     CDS             23663..24439
                     /gene="lldR"
                     /locus_tag="OQM34_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1452245.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24436..25626
                     /gene="lldD"
                     /locus_tag="OQM34_001762"
     CDS             24436..25626
                     /gene="lldD"
                     /locus_tag="OQM34_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1452246.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            25822..26295
                     /gene="trmL"
                     /locus_tag="OQM34_001763"
     CDS             25822..26295
                     /gene="trmL"
                     /locus_tag="OQM34_001763"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1452247.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26348..27169)
                     /gene="cysE"
                     /locus_tag="OQM34_001764"
     CDS             complement(26348..27169)
                     /gene="cysE"
                     /locus_tag="OQM34_001764"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1452248.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27249..28268)
                     /gene="gpsA"
                     /locus_tag="OQM34_001765"
     CDS             complement(27249..28268)
                     /gene="gpsA"
                     /locus_tag="OQM34_001765"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1452249.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28268..28735)
                     /gene="secB"
                     /locus_tag="OQM34_001766"
     CDS             complement(28268..28735)
                     /gene="secB"
                     /locus_tag="OQM34_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1452250.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(28798..29049)
                     /gene="grxC"
                     /locus_tag="OQM34_001767"
     CDS             complement(28798..29049)
                     /gene="grxC"
                     /locus_tag="OQM34_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1452251.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29191..29622)
                     /locus_tag="OQM34_001768"
     CDS             complement(29191..29622)
                     /locus_tag="OQM34_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1452252.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            29867..31411
                     /gene="gpmM"
                     /locus_tag="OQM34_001769"
                     /gene_synonym="pgmI"
     CDS             29867..31411
                     /gene="gpmM"
                     /locus_tag="OQM34_001769"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1452253.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31421..32704
                     /gene="envC"
                     /locus_tag="OQM34_001770"
     CDS             31421..32704
                     /gene="envC"
                     /locus_tag="OQM34_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1452254.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32708..33667
                     /locus_tag="OQM34_001771"
     CDS             32708..33667
                     /locus_tag="OQM34_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1452255.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
BASE COUNT         8381 a         8838 c         8598 g         7883 t
ORIGIN      
        1 ggtggctgga tctggtggct atcttccgta aacgggaata cctgccctgg cgggtccagt
       61 atgtgctgga tgaaatgctg cgtgaaattc gccaccagct tgctcagtcg cagcaactgc
      121 ggcctgaaca agcggcggaa agcgaagatt aatgatcgag ataaaggtaa tgcacccagc
      181 tggtcatgcg taatagcact ttgcgcatca ccgagacatg gctgaaatga tcagagtaga
      241 ccgccacctg ggcgtaaata ccgtctggta aagcgctcaa atcagtgtgc tcatccagtt
      301 caatggtcgc gataacgcca tctgaacccg gcgctgtgtt taacgtctgt aaggtgccgg
      361 tcgactgata agctccgccg ggaacagcag gactaatggc tgccagcttg ccgctgaata
      421 ccttacctgg cagagcatta aacaccactt ccgcatcgtc gccaggggcc agtcgcagca
      481 aggagttctg acggaactgc gccacaattt gtcgtttctg atcgggtata aacaccatca
      541 ccggacgtag cggcagcgac gcggcatagg tacccggacg aatcagcacc tgggtcacat
      601 aaccatcgct cggcgcacgc actatcgtct gctcagggtt atattttgct tccgcgagct
      661 gcgctttcag gctggcgatt tgagaatgtt cacccaacac caggctatcc agctggctct
      721 ggatctgttt ttgttccgcc gccgatgact ttacggaggc ttcctgcgcc agataatttt
      781 gccgcgccac atcaatatcg cgttctgaaa acgggtttac tttcgcctgg ctgccgcgtg
      841 cgtaacgctg atactcttta gcgaatttat cccgcgtggc ctttgcctgc tgagtattcg
      901 ccgccatctc atctaactct gcgcccaacg cccgctgttt atgttctgcg gtaacgatat
      961 ccgccatcag ccgatccacc cgcgcctgat aacgcgtcgg gtccagtcga aatagcacct
     1021 cacctttttt aatcagcgta tttttcttat ccgtcacttc gatcaccacg cctgtcacct
     1081 gtgggacaac aggaatagaa ataaccgctt tttgcgcctt gaaggtatac ggatggttat
     1141 agttcattaa cagaattaaa ccactgacaa taaatatgcc acccagggcc gctgtggaaa
     1201 tggtccattt atttacagga attttgaaga tcttaaacat tgcccatgca aaagccacat
     1261 aggtcaaaat aatcagtaaa tccataagta ttactcggca gaagaggatt tgatgtcggc
     1321 gagctgtttc tccagcccgg caatacgctg ttgtagttgc acaacggagg agtcatggat
     1381 ttgcattccc catccccgct ccgggcggta aagcgtggcc caaatccaca aaaatggcca
     1441 gataacatgc agcgtaaaaa gactcaccca accagcaaca tgaatggcgt cggcatgagg
     1501 atgattacgt tttttagcaa tcaggtaggg aatatcatgt aaaataataa tcccatagaa
     1561 tatcaccaga aatacaaaaa taagcactcc cagtgcaaaa tagtccagga acatatttcc
     1621 ctcaaagaat ataaaaaaga acaattaacg catattatga aatgccatga tgcagtgcaa
     1681 tttccttatt tatataacga catattgttt tttatatact ttctgaaatg tgctgtctgg
     1741 tttttcaaca ggacgaggtt tatcatattg aaatagtgga actattggcc aaactaatga
     1801 ataactccag taaaacatcc acagtacaga ttaagcgtat taaaccttca attatctacc
     1861 gtttattgct gattggcctc ggatcaccaa tggtgattta cggcctggtt cgcccgctca
     1921 ccatcgaaac gcgagattaa actcctgaca aaacacgccc cagaaacgca cttctgttgg
     1981 cgtagtctgg gttattgcgc acgtaggttt aaaacgtaaa ttgtaacaac gtgcgctttg
     2041 tttatgccgg atgcggcgtg tacgccttat ccggcctacg gtcaggttcc cgtaggcatg
     2101 ataagacgcg taagcgtcgc atcaggcaat gaatacccaa tgcgaccagc ttcttatatc
     2161 agaacagccc caacggttta tccgagtagc tcaccagcag gcacttggtt tgctggtaat
     2221 gctccagcat catcttgtgg gtttcgcgac cgatacctga ttgtttgtag ccaccaaacg
     2281 cagcatgtgc cgggtaagcg tgataacagt tggtccacac gcgcccagcc tgtatgccgc
     2341 gccccatctt ataggccaga ttaccgttgc ggctccagac gcccgcgccc aggccatatt
     2401 gcgtatcgtt cgccagctcc agcgcttctt ccatcgtttt gaaggtggtc accgccagca
     2461 ccgggccaaa aatctcctcc tggaacaccc gcatattgtt ctgaccaaac agaatcgtcg
     2521 gttcgaggta gtagccgtct ttcagttcac cttccagcag cttgcgccgc ccgcctgtga
     2581 gcacgtcagc gccctctttt ttaccgatat caatgtagtt gaggatggtt tccagttgcc
     2641 cgtgagaaac ctgcgcgccc atttgcgtca cgctgtcgag cgggttaccg ctgcgaatgc
     2701 tttcgacacg gcggatggcg cgttccataa agcgttcgta gattgactcc tgcactaagg
     2761 cacgactcgg acaggtgcaa acttcgccct ggttaaaggc aaacagtgca aagccttcca
     2821 gcgctttatc gaaaaaggca tcttcttcat ccatcacatc agcaaagaag atatttggcg
     2881 acttaccgcc caactccagc gtcaccggaa taatgttttg cgttgcgtat tgcataattt
     2941 gttggcccac ttccgttgag ccggtaaacg ccactttggc gatgcgtttc gaggtcgcca
     3001 gatattcgcc aatttcccca cctgcgccat tgaccacgtt caccacgccc ggcggcagta
     3061 aatcaccgac aatttccatt agcagcagta cagaaagcgg ggtaagacgt gcgggtttca
     3121 gcaccacaca gttgcccgcc gccagcgcgg gagccatttt ccagctcgcc atcagcagcg
     3181 ggaagttcca cgggataatc tgccccacca cgcctaacgg ttcatggaaa tgataggcca
     3241 cggtttcgct atcaacttca ctgatcccac cttcctgcgc ccgaatacac gaggcgaaat
     3301 agcggaaatg gtcaatcgcc agcggtacat ccgcagcact ggtttcgcga atgggtttgc
     3361 cgttatccca ggtttcagct gtcgctaaca gctcgaggtt ttgttccatt cgatcggcaa
     3421 tcttaaacag aatcgccgcg cgatcctgca ccgaggtgtg cgcccattta tctttcactt
     3481 tgtgcgcagc atccagcgcc agatcgatgt ctcgtttgcc cgaagacgcc acttcgcaca
     3541 gcagctgccc ggtcaccggc gtcagattct ggtaatactc accatcggca ggggctaccc
     3601 attcaccgcc aataaagttg tcgtagcggg cttttaactt gatggggaaa ccatactcgc
     3661 cgggcttaat ctgtgctgaa gggggattat tggtcatgat cgtctccttg cggtgtgagg
     3721 tataacaagg gtagacgtga ctggcgaaat cttcgccagt cggtaacagc tttacgacag
     3781 ctatcacgaa tttacgggca aggattacag agatagttga aaaagcggct gacaatttgc
     3841 cagccgttgt tggaatgatg attacattgc ggcgcgataa atcaccacaa tttcttcgtg
     3901 agttgcctgg atcgggttag taaagccaca ggcatctttc agggcattag tcgccagaac
     3961 cgcgaaatct tcttctttca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     4021 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     4081 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     4141 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     4201 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     4261 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     4321 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     4381 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     4441 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     4501 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     4561 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     4621 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     4681 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     4741 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     4801 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     4861 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     4921 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     4981 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     5041 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     5101 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     5161 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     5221 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     5281 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     5341 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     5401 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     5461 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     5521 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     5581 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     5641 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     5701 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     5761 gcgcatccct tcgccgttga gctggcgcgc ccaggcgaaa tcaggaagat taaccgcgcc
     5821 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     5881 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     5941 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     6001 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     6061 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     6121 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     6181 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     6241 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     6301 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     6361 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     6421 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     6481 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     6541 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     6601 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     6661 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     6721 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     6781 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     6841 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     6901 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     6961 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     7021 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     7081 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     7141 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     7201 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatggcctc tgcgctgcgg
     7261 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     7321 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     7381 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     7441 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     7501 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     7561 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     7621 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     7681 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     7741 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     7801 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     7861 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     7921 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     7981 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     8041 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     8101 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     8161 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     8221 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     8281 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     8341 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     8401 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     8461 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     8521 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     8581 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     8641 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     8701 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     8761 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     8821 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     8881 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     8941 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa tagaaagttt
     9001 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     9061 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     9121 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     9181 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     9241 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     9301 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     9361 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     9421 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     9481 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     9541 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     9601 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     9661 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     9721 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     9781 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     9841 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     9901 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg
     9961 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
    10021 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
    10081 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
    10141 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
    10201 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
    10261 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
    10321 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
    10381 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
    10441 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
    10501 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
    10561 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
    10621 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
    10681 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
    10741 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
    10801 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
    10861 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
    10921 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
    10981 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
    11041 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
    11101 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
    11161 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
    11221 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
    11281 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
    11341 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    11401 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    11461 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    11521 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    11581 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    11641 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    11701 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    11761 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    11821 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    11881 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    11941 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    12001 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    12061 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    12121 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    12181 catctccgct atctgcgggc gatgtgacta acgacctgag ccacatacgt aaaatcatcg
    12241 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    12301 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    12361 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    12421 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    12481 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    12541 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    12601 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    12661 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    12721 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    12781 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    12841 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    12901 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    12961 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    13021 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    13081 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    13141 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    13201 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    13261 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    13321 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    13381 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    13441 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    13501 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    13561 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    13621 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    13681 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    13741 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    13801 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    13861 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    13921 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    13981 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    14041 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    14101 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    14161 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    14221 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    14281 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    14341 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    14401 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    14461 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    14521 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    14581 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    14641 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    14701 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    14761 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    14821 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    14881 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    14941 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    15001 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    15061 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    15121 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    15181 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    15241 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    15301 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    15361 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    15421 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    15481 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    15541 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    15601 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    15661 aaatgggcct ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga
    15721 ctggttcgat cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
    15781 ctgtctactc gcctaattac caaacaattc actcccttta cttcgattta ccgctggttg
    15841 atattctgaa aatgcatttt taccgaaata gccatctata aacaataaaa atatatagaa
    15901 gatatcattt gttaattaac aagcatttac gtctcttttt ttcggattta attgtgaaat
    15961 tttaatcatc atattcacaa aatcaattat ctgtgaaatt tttcccagac atggtttctt
    16021 tcatatttga catttattaa taatcatcat tagaatccac gaaaaataat atattcatac
    16081 aaacacagac atcttttgaa acgaatgata catttttatg atgaatgctt aaaacaatat
    16141 ttaatcgaca cacatcactg tcattaatgt aaagcaacat agctcaccaa taatacaatg
    16201 gacaactcta ccggcattga tcgctggtcg gaggaaataa ggatttttac atgaacttga
    16261 agaagatttt tttcagtgct gtcactgttt cagttctctg cgcattaacg ggatgtgact
    16321 acattgaaga aggcaagcca gaaagtagtt tactgaaaca acaagaagaa cataataata
    16381 aaattgtgct acttgaaaaa caacaagcac agctaaagag tcaactcgaa actattcaaa
    16441 aacaacaaac tggcataata aacagtacaa agactttaac tcacgtaatt aaatccgtta
    16501 aagaccaaca aaatactttt atttttaccg agtttaaccc agcaaaaacg aagtatttca
    16561 tcctgaacaa cggttcggtg gctttagcgg gtcgagtgtt atccattgac gccacagaga
    16621 atggtagtgt tattcatatc tcactggtca acttattaag tacacctatc tcaaacattg
    16681 gttttaatgc gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc
    16741 aacaattact tttcaacaca tcaatgaaat ccacattgaa attattacca ggtcaatggc
    16801 aagacattaa tttgaccctg aagggtgtat cgcccaataa cctgggatat ctgaagttag
    16861 ccatcaacat ggaaaatatt cagttcgaca atcttccctc tgctgaaaat cggcagaaaa
    16921 gaagcaaaaa ataaatgcag ttagtacgct tataacaagg aatagcgagc atcattaatg
    16981 aacaaaatat ttaaagttat ctggaaccct gcgacaggga attatactgt taccagcgaa
    17041 acggcaaaaa gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt
    17101 gcaggtggaa tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga caacggtcag
    17161 ggtgttgatt acggtagtgg atcagctggc gacggctggg ttgctatagg caaaggggcg
    17221 aaagcaaata cttttatgaa caccagtggt tccagtactg ctgtgggtta tgacgctata
    17281 gctgaaggcc aatatagctc tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca
    17341 atggcctttg gggttagtgc aatatcagaa ggcgatagaa gtatagcact gggtgcctct
    17401 tcgtattcat tgggccaata ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa
    17461 ttgtctattg ctatggggga ctcttccaaa gcggaaggag caaacgccat tgccctgggg
    17521 cgtagcagtg tagctagcgg tactgacagc ctcgcatttg gcagacaatc acttgccagc
    17581 gcagcgaacg ctattgcgat aggtgctgag accgaagccg ctgaaaatgc aactgctatt
    17641 ggcaataatg cgaaggcaaa agggactaat agcatggcaa tggggttcgg aagccttgcc
    17701 gataaagtca atactatcgc attaggaaat ggcagccagg ctctggcaga taatgcaatc
    17761 gccataggcc agggcaacaa agctgatggc gtggatgcca tcgctctggg taatggtagc
    17821 cagtcgagag gcttaaacac cattgcctta ggcacagcca gtaatgcaac tggtgataag
    17881 agtcttgcgc ttggtagtaa tagcagtgcc aacggtatta actctgtcgc gctgggcgca
    17941 gattccattg cggatttaga caataccgtc tctgtcggca atagttcatt aaaacgcaag
    18001 atcgttaatg tgaaaaatgg cgcgatcaag tctgacagtt acgatgccat taatggttca
    18061 cagctttatg ccattagcga ctcggtagca aaaaaggctt ggaggagggg ctgcagtaga
    18121 tgttgatgac ggtactgtta cagcaccaac ctacaattta aaaaatggta gcaaaaataa
    18181 cgtaggggct gcgctcgctg tacttgatga aaacaccctg caatgggacc aaaccaaagg
    18241 caaatacagc gctgctcatg gtactagtag cccaactgcc agcgtaatca ccgatgttgc
    18301 ggatggcacg atttcagcct ccagtaagga tgcggttaac ggttcccaac tgaaagctac
    18361 caatgacgat gtcgaagcca acaccaccaa tatcaccaac ctgacggatt ccgttggtga
    18421 ccttcaggct gatgccctgc tctggaacga aactaaaaag gcattcagtg cagctcacgg
    18481 ccaggatacc accagcaaaa tcaccaacgt taaagatgcc gacctgacgg ctgacagcac
    18541 tgatgctgtt aacggctctc agctgaaaac caccaacgat gctgtggcga cgaataccac
    18601 caatatcgcc aataacactt ccaatattgc cactaacacc accaacatct ctaacctgac
    18661 tgagacggtg actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtatt
    18721 cacggcagct catggcaccg agaccaccag caaaataacc aacgttaaag atggcgacct
    18781 gacgactggc agcaccgatg ccgttaacgg ctctcagctg aaaaccacca acgatgccgt
    18841 ggcgacgaat accaccaata tcgccactaa caccaccaac atctctaatc tgactgagac
    18901 ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg tcttcactgc
    18961 agctcatggc aacaataccg ccagcaaaat caccaatatc ctggacggca cagtcactgc
    19021 aaccagttcc gatgccatta acggtagcca gctttatgac ttaagcagca atatcgccac
    19081 ctacttcggc ggcaatgctt ctgtgaatac tgacggtgtg tttaccggtc caacctacaa
    19141 aatcggtgaa acaaattatt ataacgtcgg cgatgcactg gctgcgatta actcctcatt
    19201 tagcacgtct ctcggcgatg ctctgctttg ggatgccacc gcaggtaaat tcagtgccaa
    19261 acacggtact aatggtgacg caagcgtgat cactgatgtc gcagatggtg aaatttcaga
    19321 ctccagttct gacgcagtaa acggctcaca actccacggc gtgagcagtt atgttgttga
    19381 tgcgctgggg ggtggtgccg aagtcaatgc agacggcacc atcactgcgc cgacgtacac
    19441 cattgctaat gctgattacg ataatgtcgg tgatgccctg aatgctatcg ataccactcc
    19501 tgacgacgct ctgctctggg atgcggacgc cggtgaaaat ggtgcattta gcgccgctca
    19561 cggaaaagat aaaactgcca gtgtaatcac taacgtcgct aacggtgcaa tctctgctgc
    19621 cagcagcgac gcgattaacg gctcacaact ctataccacc aataagtaca tcgctgatgc
    19681 gctgggtggt gacgcagaag tcaacgctga cggcaccatc accgcaccga cttacaccat
    19741 tgcgaacgcc gagtacaaca acgtcggtga cgccctggat gcgcttgatg ataacgccct
    19801 gctgtgggat aagactgcca atggcggtgc tggagcctac aatgccagcc atgacggtaa
    19861 agccagcatc atcactaatg tcgctaatgg cagtattagt gaggacagta ccgatgcagt
    19921 gaacggttct cagttgaatg cgacgaatat gatgattgag cagaacaccc aaattatcaa
    19981 tcagctcgct ggtaacaccg acgcaaccta tatcgaagaa aatggcgctg gtatcaacta
    20041 cgttcgtact aacgacaacg atttagcctt taacgatgca agcgcctctg gtgtcggcgc
    20101 tacagctgta ggttataacg ctgtcgcgtc tggtgccagc agcgtagcca ttggtcagaa
    20161 cagcagcagc accgttgata ccggtattgc gctgggtagc agctccgttt ccagccgtgt
    20221 gattgccaaa ggttctcgtg acactagcgt aacggaaaat ggcgtggtga ttggttatga
    20281 caccactgac ggcgaactgc taggtgcatt gtcaattggt gatgacggta aataccgcca
    20341 aatcatcaac gtagctgatg gttcagaagc ccatgacgcc gttacggttc gccagttgca
    20401 gaacgctatt ggagcggtcg ccactacgcc aaccaagtac tttcacgcca actcaacggc
    20461 agaagactca ctggccgttg gtgaagactc gctggcaatg ggtgcgaaaa ctgtcgttaa
    20521 tggtaatgca ggtattggta tcggtttgaa cactctggtt ctggctgatg cgatcaacgg
    20581 cattgctatc ggcagtaacg cacgcgcaaa ccatgcaaac agtatcgcaa tgggtaacgg
    20641 ttctcagacc acccgtggtg cacagactgg ctacaccgcc tacaacatgg acgcaccgca
    20701 gaactctgta ggtgagtttt ctgtcggtag tgaagacggt caacgtcaga tcacaaacgt
    20761 cgcagctggt tcagcggata ccgatgcggt taacgtgggt cagttgaaag tcactgatga
    20821 gcgcgtagcg caaaataccc agagcattac taacctgaac aatcaggtca ctaatctgga
    20881 tactcgcgtt actaatatcg aaaacggtat tggcgacatt gtcaccaccg gtagcaccaa
    20941 gtacttcaag accaacaccg atggcgtaga tgccaacgcc cagggtaaag atagcgttgc
    21001 tattggttct ggttccattg ctgccgctga caacagcgtc gcactgggta ccggttccgt
    21061 tgcagaggaa gaaaatacaa tctctgtagg ttcttctact aaccaacgcc gtatcaccaa
    21121 cgtagctgcc agtgttaatg ccaccgatgc ggttaacgtg tcgcagctga aatcttctga
    21181 agcaggcgga gtgcgctacg acaccaaagc tgatggttct atcgactata gcaatatcac
    21241 cctcggtggt ggcaacggca gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa
    21301 caacgacgcg gtgaactacg cgcagttgaa gcaaagcgcg caggaaacga agcaatacac
    21361 cgatcagcgg atggttgaga tggataacaa actgtctaaa actgaaagca agttgagcgg
    21421 tggtatcgct tctgcaatgg caatgaccgg tctgccgcag gcttatacac cgggtgccag
    21481 catggcttct attggtggcg gtacttacaa cggtgaatcg gcagttgctt taggtgtatc
    21541 gatggtgagc gccaatggtc gttgggtcta caaattacaa ggtagtacca atagccaggg
    21601 tgaatactcc gccgcactcg gtgccggtat tcagtggtaa tgatccatta agttagtgtg
    21661 actaagggca aacgccaaat tgcctgatgc gttacgcgca tcaggcaaga taaggcgctc
    21721 cgggtcaaca atctaagggc aattctctga tgaggattgc ccttttcttt accagacatc
    21781 tccccccaca agaattggcc ctaccaattc ttcgcttatc tgacctctgg ttcacaattc
    21841 cccaattaaa actcacatca atattgccaa tacataacat ttagttaacc attcattgtc
    21901 attatcccta cacaacacaa ttggcagtgc cacttttaca caacgtgtga caaggagatg
    21961 agcaacagac tcattacacg atgtgcgtgg actctaggag acctgcaatg aatctctggc
    22021 aacaaaacta cgatcccgcc gggaatatct ggctttccag tctgatagca tcgcttccca
    22081 tcctgttttt cttctttgcg ctgattaagc tcaaactgaa aggatacgtc gccgcctcat
    22141 ggacggtagc aatcgccctt gccgtggctt tgctgttcta taaaatgccg gtcgctaacg
    22201 cgctggcctc ggtggtttat ggcttcttct atggtttgtg gcccatcgcg tggatcatta
    22261 ttgcagcagt gttcgtctat aagatctcgg tgaaaaccgg gcagtttgac atcattcgct
    22321 cgtctattct ttcgataacc cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt
    22381 tcggcgcttt ccttgaagga gccgcaggct ttggcgcacc ggtagcaatt accgccgcat
    22441 tgctggtcgg cctgggtttt aaaccgctgt acgccgccgg actgtgcctg attgttaaca
    22501 ccgcgccagt ggcatttggc gcgatgggca ttccaatcct ggttgccgga caagtaacag
    22561 gcatcgacag ctttgagatt ggccagatgg tggggcgtca gctaccgttt atgaccatta
    22621 tcgtgctgtt ctggatcatg gcgattatgg acggctggcg cggtatcaaa gagacgtggc
    22681 ctgcggtcgt ggttgcaggc ggctcgtttg ccatcgctca gtaccttagc tctaacttca
    22741 ttgggccgga gctgccggac attatctctt cgctggtatc actgctctgc ctgacgctgt
    22801 tcctcaaacg ctggcaacca gttcgcgtat tccgcttcgg tgacttgggg gcgtcacagg
    22861 ttgatatgac gctggcccac accggttaca ccgcgggtca ggtgttacgt gcctggacac
    22921 cgttcctgtt cctgaccgct accgtaacac tgtggagcat cccgccgttt aaagccctgt
    22981 tcgcatcggg tggtgcgctg tatgagtggg tgatcaacat tccggtgccg tacctcaata
    23041 aactggttgc ccgtatgcca ccagtggtca gcgaggctac agcctatgcc gccgtgttta
    23101 agtttgactg gttctctgcc accggcaccg ccattctgtt tgctgcactg ctctcgattg
    23161 tctggctgaa gatgaaaccg tctgacgcta tcagcacctt cggcagcacg ctgaaagaac
    23221 tggctctacc catctactcc atcggcatgg tgctggcgtt cgcctttatc tcgaactatt
    23281 ccggactgtc atcaacactg gcactggcac tggcgcacac cggtcatgca ttcaccttct
    23341 tctcgccgtt cctcggctgg ctgggggtat tcctgaccgg gtcggatacc tcatctaacg
    23401 ccctgttcgc cgcgctgcaa gccaccgcag cacaacaaat tggcgtctct gatctgttgc
    23461 tggttgccgc caataccacc ggtggcgtca ccggtaagat gatctccccg caatctatcg
    23521 ctatcgcctg tgcggcggta ggcctggtgg gcaaagagtc tgatttgttc cgctttactg
    23581 tcaaacacag cctgatcttc acctgtatgg tgggcgtgat caccacgctt caggcttatg
    23641 tcttaacgtg gatgattcct taatgattgt tttacccaga cgcctgtcag acgaggttgc
    23701 cgatcgtgtg cgggcgctga ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc
    23761 tgagcgccaa ctggcgatgc aactcggcgt atcacgtaat tcactacgcg aggcgctggc
    23821 aaaactggtg agcgaaggcg tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg
    23881 gcgtcatgac acatggtcgg agcaaaacat cgtccagccg ctaaaaacgc tgatggccga
    23941 tgatccggat tacagtttcg atattctgga agcccgctac gccattgaag ccagcaccgc
    24001 atggcatgcg gcaatgcgcg ccacacctgg cgacaaagaa aagattcagc tttgctttga
    24061 agcaacgcta agtgaagacc cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc
    24121 gattgccgaa gcctcacata acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt
    24181 cctgcaatcc tcagtgaagc atagccgtca gcggatgtat ctggtgccac cggttttttc
    24241 acaactgacc gaacaacatc aggctgtcat tgacgccatt tttgctggtg atgccgacgg
    24301 ggcgcgtaaa gcaatgatgg cgcaccttag ttttgttcac accaccatga aacgattcga
    24361 tgaagatcag gctcgccacg cacggattac ccgcctgccc ggtgagcata atgagcattc
    24421 gagggagaaa aacgcatgat tatttccgca gccagcgatt atcgcgccgc agcgcaacgc
    24481 attctgccgc cgttcctgtt ccactatatg gatggtggtg catattctga atacacgctg
    24541 cgccgcaacg tggaagattt gtcagaagtg gcactgcgcc agcgtattct gaaaaacatg
    24601 tccgacttaa gcctggaaac gacgctgttt aatgagaaat tgtcgatgcc ggtagcactg
    24661 gcaccggtgg gtttgtgtgg catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg
    24721 gcggacgcgc atggcattcc gtttactctc tcgacggttt ccgtttgccc gattgaagaa
    24781 gtcgcgccag ccatcaagcg cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc
    24841 tttatgcgta acgcgctgga gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc
    24901 gtggatatgc cgacaccggg cgcacgctac cgtgatgcgc attcaggtat gagcggccca
    24961 aatgcggcaa tgcgccgcta cttgcaggcg gtgacgcatc cgcaatgggc gtgggatgtg
    25021 ggcctgaacg gtcgtccaca tgatttaggt aatatctcag cttatctcgg caaaccgacc
    25081 ggactggaag attacatcgg ctggctgggg aataacttcg atccgtccat ctcatggaaa
    25141 gaccttgagt ggatccgcga tttctgggat ggcccgatgg tgatcaaagg gatcctcgat
    25201 ccggaagatg cgcgcgatgc agtacgtttt ggtgctgatg gaattgtggt ttctaaccac
    25261 ggtggccgcc agctggacgg tgtactctct tccgctcgtg cgttgcccgc tattgcagat
    25321 gcggtgaaag gtgatatcgc cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc
    25381 gtgcgtatga ttgcgctcgg tgccgacacc gtactgctgg gtcgtgcttt cctgtatgcg
    25441 ctggcaacag cgggccaggc gggtgtagct aacctgctaa atctgatcga aaaagagatg
    25501 aaagtggcga tgacgctgac tggcgcgaaa tcgatcagtg aaattacgca agattcgctg
    25561 gtgcaggggc tgggtaaaga gttgcctacg gcactggctc cgatggcgaa agggaatgcg
    25621 gcatagtcgt ttgcgccccc tcaccctaac cctctccctc agggagaggg gaccgttcgg
    25681 cgctgtatgt actccctcac tctgaaacga caccgcactc tttttttctc cctcgcccct
    25741 ccggggaggg ccggggtgag gggaaaaggc cgcacggttc accaattttc tgctatcctg
    25801 cccccgcact aagggggcag tatgctaaac atcgtacttt acgaaccaga aattccgcca
    25861 aatactggca acatcatccg tctttgcgct aataccggct ttcgtctaca tatcatcgaa
    25921 ccgatgggat ttgcctggga cgacaagcgc ctgcgccgcg cggggctgga ctatcacgag
    25981 tttaccgccg ttacgcgtca tcatgactat cgcgcgttcc tcgaagcaga aaatccccag
    26041 cgcctgtttg ccctcaccac gaaaggtacg cctgctcaca gcgccgtaag ctatcaggat
    26101 ggcgattatc tgatgttcgg cccggaaaca cgcggcctgc cagcgagcat tcttgatgcc
    26161 ctgcccgctg aacaaaaaat tcgcattccg atggtgccgg acagccgcag catgaatctg
    26221 tccaatgcgg tgtcggtagt ggtgtatgaa gcctggcggc agctgggata tccgggagcg
    26281 ttgttgagag attagttact gtatgccgga tagatgatta catcgcatcc ggcacgatca
    26341 caggacatta gatcccatcc ccatactcaa atgtatggtt aataccgttg aaatgctggt
    26401 ccatatccat tgatggctta tcgctgtctg gtttaccgac aatacgaacc ggaacgccag
    26461 cggcggttgt atgcggcggc accggttgca gcaccacgga acctgcgcca atcttcgcgc
    26521 cgcgaccaac ttcaatattg ccgaggattt tcgcgcccgc gccaatcatc acaccttcac
    26581 gaattttcgg gtgacggtca ccaccagatt tacccgtacc gccaagcgtc acagattgca
    26641 gaatcgatac gtcgttttca attaccgccg tttcacccac aacgatgccc gtggcgtggt
    26701 cgagcataat gccacggcca atttttgctg ccgggtgaat atcgacctgg aacgtcacag
    26761 aaacctggtt ttgcagaaag attgccaatg cgcgacgccc ctgattccac aaccagtgac
    26821 cgatgcgata ggcctgcaag gcatgaaaac ccttcaggta taacaacggg gttgagtatt
    26881 tatcgactgc cgggtcgcgg gtacgcaccg cctgaatatc acaggccgca gaggcgatca
    26941 tttccgggtc agcggcgtag gcttcttcaa ccacttcacg gatagcaata gcaggcataa
    27001 ttggcgatga cagcttgttc gccagcatgt agctcagtgc actgccaagg ttttcgtgct
    27061 tgagtagcgt cgcgtggtaa aaactggcca gcattggctc acagtccgcc agcgttctgg
    27121 cttcggcttt aatattgttc cagacaattt ccagttcttc acacgacatt gcttactcca
    27181 cacgatgaga taatgaccgg cccgttctgc gcgggccggg tcatagcggt aacaaaggtt
    27241 ccctggggtt agtgactgct gcgctcgtcc ttgcgtgcac gacctaataa tgtcaatgct
    27301 gcctcgcgcg cgttttttcc gcaatataat acttgataaa tttcctcggt tattggcatt
    27361 tcaacgccga agcgatgcgc cagttcgcgg acttctttcg tattgcggta gccttccacc
    27421 acctgaccaa tcttctcctg cgcgctttgt acatccatgc cctgaccgag catcatacca
    27481 aaacggcggt tacgcgactg gttgtcggta caggtaagca ccagatcgcc cagccccgcc
    27541 atgcccataa aggtggcagg atcggcaccc agcgccgcac caagacgcga catttcagcc
    27601 agcccacggg tgatcagcgc cgtacgcgca ttcgcaccaa aaccgatacc gtcagacatc
    27661 cccgcgccaa tggcaatgac gtttttcacc gcgccgccaa gctgcacgcc aatgaaatcc
    27721 gggttgctgt aaacgcggaa acttttgccg cagtgcagca gctgctggag atcatcggca
    27781 aaggtctgat cggtcgaggc cagcgaaata gctgtcggta aacctgccgc cagttctttc
    27841 gcaaacgttg ggccagagat aaccgccagc ggaatttgat cgcctaaggc ctcacgcgcc
    27901 acgtcctgta acagacgtcc ggtttccgct tccagccctt tggtcgccca caccagacgc
    27961 gcatcaggac gcatcagcgg tttaatctgg cgcagcactt caccaaagac atggctgggt
    28021 acgacgacga gaatattacg actggctgcc agcgcagtgg cgagatcgct ttcaagatgg
    28081 agcgtatcgg gaaaaggcac atcggggaga aacgcggcgt tacagcggtc gcgttcaagc
    28141 gttgcgatat gttcagggtc atggccccag aggacaacct cgtggccatt tcttgccagg
    28201 gtaatggcaa gagcggtgcc gtacgagccg gcaccgatca cagtcattga agcattacgt
    28261 tggttcatca ggcatcctga tgttcttcag taccttcgcc agcctgctgc tgcaaatagt
    28321 tcatgaacag cgcatcgaag ttaaccggcg caaggttcag ttgcgggaat gtaccgcggg
    28381 ataccatgct ggtgatgcac tcacgagcat acgggaacag aatgttcggg cagtatgcgc
    28441 ccaggcaatg tgccatctgg gtgccttcga tacccgcgat ggagaaaata ccgccctgct
    28501 gaacttcaca caggaacgcg gtttcttcgc ccaaggaggc cgttacggta acacgcagta
    28561 ccacttcgta tacgtcatct gccagttggg aagatgccgt atccagatca agtttaactt
    28621 ctggttgcca atctttctgg aaaacgtgcg gcgcgttcgg cgcttcgaaa gagatatcct
    28681 tggtataaat acgttggatc tggaaagtca tttcagtgtt gttttgttct gacatgtgta
    28741 gaaaaccctt aagtgttgtc cttaaatact gcgcaatgta ataccaacgt tcacacgtta
    28801 tttcagcagg ggatccagtc caccacgtgc atccaatgca tacaagtcat cacagccgcc
    28861 aatgtgctgt gcgtcaataa aaatctgggg aaccgtggtg caaccgctgc gtttgatcat
    28921 ctcttcacgc ttggcggcat tgccatcgat cggcaactcc tggaaactca cgcccttgct
    28981 gctcagcagt gcttttgcac gatggcaata cgggcaggtt tctttggtat agatttcaac
    29041 attggccata atttagctcc tgttttttta ccctgaaatt tcatgttgca gggcggctgc
    29101 aaaatggagt gtccccagaa gcttataaca ataagtcgct gggggcctaa ttgcagctaa
    29161 cggcctgcat catgaaagac gacaggtaaa ttatttgccg cgcaccagag gcaagttttc
    29221 gccagcccag ccagcaacgc cctctttcag cacgaatact tgcgcaaaac cagctttcgt
    29281 cagcgcgttt gcaggctcct ggcactgcat gccagaaccg tctaccacga taaccgattt
    29341 gtctttgtgc ttctcaagct caccaacatt gttggctttg atttcgctcg gcaacagatt
    29401 gatagaacct gcgatatggc ctttacggaa gtcatcacgc tgacgtaaat ccacaaccac
    29461 agcgtcttct ttgttgatca gacgcgtagc ttcaccacga gtaatcacct tcactttcga
    29521 ggtcaggctc ttaaacgtag tcacaagaac cgccaccagt aacgcgatcc aggcgatact
    29581 cagtatggga tgacggccaa caaattgcat aatttcttgc atggggggta acaactcccg
    29641 acgtagtgat taaaaaaacc aggaaaggag tatacctgcg cggtgcggca aatacagcca
    29701 gcgcgttaac tggaatgcaa ttttgcgggg cacgacgaaa aaaaagcgca aatctggcat
    29761 cggcttgccg cgagcggtcg tatattttga tcttcagagg ctattttatc gattcagctg
    29821 tagtaaaatt acgcaaattt tgactcttga gtatgaggtt gtcgcaatgt cggtttctaa
    29881 aaaacctatg gtactggtga ttctggatgg ctatggctat cgtgaagaac agcaggataa
    29941 cgccattttt agtgctaaaa ccccggtaat ggatgcactg tgggccaatc gtccgcatac
    30001 cctgatcgac gcttccggtc tggaagtcgg tctgcctgac cgtcagatgg gtaactccga
    30061 agtaggccac gttaacctgg gtgccggccg catcgtgtat caggacctga ctcgtctgga
    30121 cgttgaaatc aaagatcgcg ctttctttgc taatccagtg ctggctggcg cggtacataa
    30181 agcgaaaaac gcaggtaaag cagtacacat tatgggtctg ctctccgcag gcggcgtaca
    30241 cagccacgaa gatcacatca tggcgatggt agaactggca gctgaacgcg gcgcagaaaa
    30301 aatctacctg cacgcattcc ttgacggtcg cgacaccccg ccgcgcagtg ctgaatcctc
    30361 gctgaaaaaa tttgaagaga aatttgctgc gctgggtaaa ggccgcgtag cgtccatcat
    30421 tggtcgttac tacgcgatgg accgcgataa ccgttgggat cgcgttgaaa aagcctatga
    30481 cctgctgact ctggcgcagg gtgagttcca ggccgatact gccgttgctg gtttgcaggc
    30541 agcttatgct cgcgacgaaa acgacgagtt cgtgaaagcg accgttattc gtgcagaagg
    30601 tcagccagat gcggcaatgg aagacggcga tgcgctgatt ttcatgaact tccgtgctga
    30661 ccgcgcgcgt gaaatcactc gtgcattcgt gaacgctgat ttcgatggct tcgcgcgtaa
    30721 gaaagtggtt aacgtcgatt tcgtgatgct gaccgaatac gccgctgaca tcaaaactgc
    30781 ggttgcttac ccacccgctt ccctggttaa caccttcggc gagtggatgg cgaaaaacga
    30841 caaaactcag ttgcgtattt ccgaaaccga aaaatatgcc cacgttactt tcttcttcaa
    30901 cggtggcgta gaagagtcgt tcaaaggcga agatcgcatt ctgatcaact caccgaaagt
    30961 ggctacctac gatctgcaac cggaaatgag ctccgcagag ctgaccgaaa aactggttgc
    31021 ggccatcaag agcggcaaat acgacaccat tatctgtaac tatccgaacg gcgacatggt
    31081 aggtcacacc ggggtgatgg aagcggcggt taaagcggtt gaagcactgg atcactgcgt
    31141 ggaagaagtc gcgaaagcgg ttgagtccgt gggtggacaa ctgctgatca ccgctgacca
    31201 cggtaacgct gaacagatgc gcgatccggc aacgggtcag gcacacacgg cacacaccaa
    31261 cctgccagtt ccgctgattt acgttggtga taagaacgtg aaagcggttg caggcggcaa
    31321 actttctgac atcgcgccga ccatgttgtc gctgatgggt atggaaatcc cgcaagagat
    31381 gactggtaag ccgctgttca tcgtggaata atccctcccc atgaggggaa aggcgattaa
    31441 taccatgaca cgggccgtga aaccgcgcag gtttgcaatc aggcccatca tctacgccag
    31501 cgttcttagc gctggcgtat tgttgtgcgc cttttccgcc cacgcggatg agcgtgacca
    31561 actcaaatct attcaggccg atatcgccgc aaaagagcgc gcggtacgcc aaaagcaaca
    31621 acaacgcgca agcctgctcg cacaattgaa aaagcaggaa gaagcgatct ctgaagccac
    31681 ccgtaagctg cgcgaaacgc aaaacacgct caatcaactc aataaacaga ttgatgagat
    31741 gaacgcgtcg attgccaaac tggagcagca aaaagccgcc caggagcgca gcctcgccgc
    31801 acaactggat gccgcattcc gtcagggcga gcataccggt attcagctaa ttctcagcgg
    31861 tgaagaaagc cagcgtggac agcgtttaca ggcttatttc ggctatctca accaggcgcg
    31921 acaagaaacc attgcccagt tgaagcaaac gcgtgaagaa gtcaccatgc agcgtgctga
    31981 actggaagag aaacaaagcg agcaacaaac gctgttatat gaacagcgcg cccaacaggc
    32041 gaaactgact caggctctga acgagcgtaa aaagacgctg gcggggctgg aatcttccat
    32101 ccagcaaggt cagcagcagc taagtgaact gcgtgccaac gaatcccgtc tgcgtaacag
    32161 cattgcccgt gcggaagccg cggcgaaagc gcgtgctgaa cgagaagcgc gtgaggccca
    32221 ggcggttcgc gagcgccaga aagaagcgac gcgcaaaggc accacctaca aaccgaccga
    32281 aagcgaaaaa tcgctgatgt cccgaactgg tggcctgggt gcgccgcgtg gtcaggcatt
    32341 ctggccggtt cgcgggccga cgctgcatcg ctatggcgag cagctacagg gtgaattacg
    32401 ctggaaaggg atggttatcg gtgcctctga aggtactgaa gttaaagcaa ttgccgacgg
    32461 tcgagtgatt ctggctgact ggctgcaagg ttacggtctg gtggtggtgg ttgagcacgg
    32521 taaaggcgac atgagtcttt acggctataa tcagagcgca ctggtgagcg ttggttcgca
    32581 ggttcgcgcg ggccagccaa ttgcactggt gggcagcagt ggcggtcagg gtcggccttc
    32641 actctatttc gaaattcgcc gccagggtca ggcggtcaat ccacagccgt ggttgggaag
    32701 ataagttttg tttccatttc gtcgtaacgt tcttgcattt gccgctctgt tggcgctctc
    32761 ctcccccgta cttgctggca aacttgccat cgtcattgat gattttgggt atcgcccgca
    32821 caacgaaaac caggtgctgg cgatgccttc cgctatctcc gtcgctgtat tacccgattc
    32881 accgcacgcc agagagatgg cgaccaaagc gcataacagc ggtcacgaag tgttgattca
    32941 tctcccgatg gcaccgttga gtaaacagcc gctggagaaa aatacgctac gcccggagat
    33001 gagcagcgac gaaattgagc gcattattcg tagtgcggtc aataacgtgc cctatgccgt
    33061 ggggatcaac aaccacatgg gcagcaagat gacctctaac ctgtttggta tgcagaaagt
    33121 gatgcaggcg ctggagcgtt acaatcttta cttcctcgac agcgtaacca tcggtaatac
    33181 ccaggcgatg cgcgccgcgc aaggtactgg cgtgaaggtg atcaaacgga aggtgttcct
    33241 cgacgattcg cagaacgaag cggacatccg tgtgcaattt aatcgcgcaa ttgatctggc
    33301 gcgtcgcaac ggttcaacca ttgccattgg gcatcctcac ccttcaacgg tacgcgtgct
    33361 acaacagatg gtttataacc tgccgccaga cattacgctg gtgaaagcca gcagcttgct
    33421 gaatgaaccg caggttgata cttctacacc gccgaaaaac gctgtgcctg atgcaccgcg
    33481 taatccgttc cgtggcgtga agctgtgcaa accgaagaaa ccgatagaac ctgtttatgc
    33541 taatcgcttc tttgaagtat taagcgaaag catcagccag agcacgctga tcgtttactt
    33601 ccagcatcag tggcaaggct ggggcaaaca gcccgaagcg gcgaagttta acgctagcgc
    33661 aaattaaagg cgctacgcgt aatgcgatgg tgcgaccgcg
//
LOCUS       DAJVPP010000038        33330 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVPP010000038 DAJVPP010000000
VERSION     DAJVPP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.038"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            65..1342
                     /gene="waaA"
                     /locus_tag="OQM34_001772"
     CDS             65..1342
                     /gene="waaA"
                     /locus_tag="OQM34_001772"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1452256.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1350..1829
                     /gene="coaD"
                     /locus_tag="OQM34_001773"
     CDS             1350..1829
                     /gene="coaD"
                     /locus_tag="OQM34_001773"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1452257.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1868..2677)
                     /gene="mutM"
                     /locus_tag="OQM34_001774"
     CDS             complement(1868..2677)
                     /gene="mutM"
                     /locus_tag="OQM34_001774"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS1452258.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2775..2942)
                     /gene="rpmG"
                     /locus_tag="OQM34_001775"
     CDS             complement(2775..2942)
                     /gene="rpmG"
                     /locus_tag="OQM34_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1452259.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2963..3199)
                     /gene="rpmB"
                     /locus_tag="OQM34_001776"
     CDS             complement(2963..3199)
                     /gene="rpmB"
                     /locus_tag="OQM34_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1452260.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3416..4084)
                     /gene="radC"
                     /locus_tag="OQM34_001777"
     CDS             complement(3416..4084)
                     /gene="radC"
                     /locus_tag="OQM34_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCS1452261.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4256..5476
                     /gene="coaBC"
                     /locus_tag="OQM34_001778"
     CDS             4256..5476
                     /gene="coaBC"
                     /locus_tag="OQM34_001778"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1452262.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5454..5912
                     /gene="dut"
                     /locus_tag="OQM34_001779"
     CDS             5454..5912
                     /gene="dut"
                     /locus_tag="OQM34_001779"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1452263.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6019..6615
                     /gene="slmA"
                     /locus_tag="OQM34_001780"
     CDS             6019..6615
                     /gene="slmA"
                     /locus_tag="OQM34_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1452264.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6652..7293)
                     /gene="pyrE"
                     /locus_tag="OQM34_001781"
     CDS             complement(6652..7293)
                     /gene="pyrE"
                     /locus_tag="OQM34_001781"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1452265.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7359..8075)
                     /gene="rph"
                     /locus_tag="OQM34_001782"
     CDS             complement(7359..8075)
                     /gene="rph"
                     /locus_tag="OQM34_001782"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1452266.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8202..9065
                     /locus_tag="OQM34_001783"
     CDS             8202..9065
                     /locus_tag="OQM34_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1452267.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9286..10110
                     /gene="dinD"
                     /locus_tag="OQM34_001784"
     CDS             9286..10110
                     /gene="dinD"
                     /locus_tag="OQM34_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1452268.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSITQLENSVKITEKK"
     gene            10401..11018
                     /locus_tag="OQM34_001785"
     CDS             10401..11018
                     /locus_tag="OQM34_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1452269.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11015..12697)
                     /gene="ligB"
                     /locus_tag="OQM34_001786"
     CDS             complement(11015..12697)
                     /gene="ligB"
                     /locus_tag="OQM34_001786"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1452270.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12955..13578
                     /gene="gmk"
                     /locus_tag="OQM34_001787"
     CDS             12955..13578
                     /gene="gmk"
                     /locus_tag="OQM34_001787"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1452271.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13633..13908
                     /gene="rpoZ"
                     /locus_tag="OQM34_001788"
     CDS             13633..13908
                     /gene="rpoZ"
                     /locus_tag="OQM34_001788"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1452272.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13927..16035
                     /gene="spoT"
                     /locus_tag="OQM34_001789"
     CDS             13927..16035
                     /gene="spoT"
                     /locus_tag="OQM34_001789"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1452273.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16042..16731
                     /gene="trmH"
                     /locus_tag="OQM34_001790"
     CDS             16042..16731
                     /gene="trmH"
                     /locus_tag="OQM34_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1452274.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16737..18818
                     /gene="recG"
                     /locus_tag="OQM34_001791"
     CDS             16737..18818
                     /gene="recG"
                     /locus_tag="OQM34_001791"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1452275.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18852..20057)
                     /gene="gltS"
                     /locus_tag="OQM34_001792"
     CDS             complement(18852..20057)
                     /gene="gltS"
                     /locus_tag="OQM34_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1452276.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20337..21728
                     /gene="xanP"
                     /locus_tag="OQM34_001793"
     CDS             20337..21728
                     /gene="xanP"
                     /locus_tag="OQM34_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1452277.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21849..23558
                     /locus_tag="OQM34_001794"
     CDS             21849..23558
                     /locus_tag="OQM34_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1452278.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23611..25929)
                     /gene="yicI"
                     /locus_tag="OQM34_001795"
     CDS             complement(23611..25929)
                     /gene="yicI"
                     /locus_tag="OQM34_001795"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1452279.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25939..27322)
                     /locus_tag="OQM34_001796"
                     /pseudo
     CDS             complement(25939..27322)
                     /locus_tag="OQM34_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27615..27709
                     /locus_tag="OQM34_001797"
     tRNA            27615..27709
                     /locus_tag="OQM34_001797"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27649..27651,aa:Sec,seq:tca)
     gene            28009..29193
                     /locus_tag="OQM34_001798"
     CDS             28009..29193
                     /locus_tag="OQM34_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1452280.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29605..30648
                     /locus_tag="OQM34_001799"
     CDS             29605..30648
                     /locus_tag="OQM34_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS1452281.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31163..31591
                     /locus_tag="OQM34_001800"
                     /pseudo
     CDS             31163..31591
                     /locus_tag="OQM34_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31621..32772)
                     /locus_tag="OQM34_001801"
                     /pseudo
     CDS             complement(31621..32772)
                     /locus_tag="OQM34_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8337 a         8773 c         8221 g         7999 t
ORIGIN      
        1 gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa gtcagctatt
       61 tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc tgatctggat
      121 acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg gtgaacgtta
      181 cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg tctccgtcgg
      241 tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc ctgatttacc
      301 gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg ctttcgggaa
      361 ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc gtttcctgaa
      421 taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta acctgattgc
      481 ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct ctgcccgctc
      541 ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc gtattacgct
      601 gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg caaaaaataa
      661 ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc agttggctgc
      721 taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga ttgccaccag
      781 cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt tacagcaatt
      841 cccgaattta ttgctcatcc tagtaccccg tcatccagaa cgcttcccgg atgcgattaa
      901 ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag tcccctccac
      961 cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt acggcattgc
     1021 cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc cgctggaagc
     1081 tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta aagacatttg
     1141 cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta cgcttgcaaa
     1201 agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc gtcatgccgt
     1261 tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc tggaacctta
     1321 cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat ccgggtactt
     1381 tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag atgttcgatc
     1441 acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc ctggaagagc
     1501 gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg gtcgggttta
     1561 gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt cgtggcctgc
     1621 gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc cacttaatgc
     1681 cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc tcttcatcgt
     1741 tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg gagaatgtcc
     1801 atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc catccggctc
     1861 gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca tgtttagtcg
     1921 ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa acctgcaatt
     1981 cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc gttgtaccac
     2041 cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat tcacactctg
     2101 ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac agtgattcgc
     2161 tggcatagat attccctacc cctaccacca gcttgttatc catcagccac ggtttaatcg
     2221 ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg tcgctaagcg
     2281 gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc cacagccagg
     2341 caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc accaaatcca
     2401 catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg ccagacatcc
     2461 ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta gcccgccgct
     2521 gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc ggccagcgca
     2581 agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc ggttctatgc
     2641 cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga atgaacagat
     2701 gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg tttttcgtta
     2761 catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg acgaacaact
     2821 ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt cgtagtggta
     2881 tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat acctttagcc
     2941 atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt tcagccagaa
     3001 ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg cgcagggtga
     3061 caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc aggaaacggc
     3121 gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc ttgccagtaa
     3181 cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag cttcgtatgg
     3241 ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt gaactctcga
     3301 ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata cgctgactca
     3361 gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata gcgggttaaa
     3421 tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata tggtcgagca
     3481 cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt ttatccgctt
     3541 tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc gaggcgttta
     3601 tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt agcgtgccgg
     3661 aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc accataaaga
     3721 tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc tccgggctga
     3781 gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt tcagcaatcc
     3841 cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat tgttcatatt
     3901 cagaggttaa caagccataa agagagccga aattctccag catctctttt gccagggtta
     3961 atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg acatccgtta
     4021 aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt gcattgtttt
     4081 tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt aaatcgacgg
     4141 ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg cacgacaaca
     4201 aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa tcatcatgag
     4261 cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct ataaaacccc
     4321 tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca tgaccgaagc
     4381 ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc ccgtttccga
     4441 cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg gtaaatgggc
     4501 tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg ctgccggaat
     4561 ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag ccgtgctccc
     4621 cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag aggtgcttgc
     4681 ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg gtgatatcgg
     4741 tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc atttttcgcc
     4801 cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc gtgaaccgct
     4861 cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg ctatcgccgc
     4921 cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt cactaccgac
     4981 gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag ccgccgtgaa
     5041 tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg attatcgcgc
     5101 agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg aattaacaat
     5161 aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag accatcgacc
     5221 ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc ggcaaaaacg
     5281 tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa ctcaaggatt
     5341 taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag tcttaccgct
     5401 tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc gttatgatga
     5461 aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg ctcccgactt
     5521 atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac gccgtagaac
     5581 tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt gccgatcctt
     5641 cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt atcgtgcttg
     5701 gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt tccgtgtgga
     5761 accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag atgatttttg
     5821 ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc gaccgcggtg
     5881 aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata acgtcataac
     5941 atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa gtgcttattt
     6001 tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg tcgcgaggaa
     6061 atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg tatcacgacg
     6121 gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca cttccccagt
     6181 aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat tactcgcatc
     6241 aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat tgtgttgctg
     6301 cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg tcatgcgcta
     6361 atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg tattgaagcg
     6421 cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta caccaccgat
     6481 gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc acgttttgtc
     6541 cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc gctaattgcc
     6601 gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc tttaaacgcc
     6661 aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg gcttctcttc
     6721 cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt tgtaatcacg
     6781 ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat cgagcgaaat
     6841 caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact cgcgaatcgc
     6901 cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg cgctaccaac
     6961 cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt acggcaggtc
     7021 caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga tccctttgta
     7081 agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt cagcgtaaaa
     7141 acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga agaaataggg
     7201 gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct tgctaagcgc
     7261 aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct tacttttcta
     7321 cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag cgccgccttc
     7381 tgcgtcgcta caatggattc gattccccct ctggccagcg ccaacaaggt gagtagctct
     7441 tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg cccgtcttcg
     7501 gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc cagatcgcaa
     7561 attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt catcgggttg
     7621 gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc cacgcaggca
     7681 cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca gtccagcgta
     7741 atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg ggcgatcaga
     7801 cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg agcgttacgg
     7861 gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg gccctgacct
     7921 ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt ggtatcgcca
     7981 aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag ggtaacggga
     8041 cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc ttgaaacaaa
     8101 tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag gcatcgatgg
     8161 ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc agtatgaccg
     8221 cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa atgcgctcgg
     8281 taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt agccttgaac
     8341 ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa tgtaccctgc
     8401 gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa ctggctaaac
     8461 agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa atcaacccgg
     8521 ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc gacgcgattg
     8581 ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc gcgcgcgaaa
     8641 ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc accgccgaag
     8701 tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag cgtctggtcg
     8761 cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag gagttggttc
     8821 tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg catgttaaag
     8881 agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac tttatgatgc
     8941 aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc gaagtgacaa
     9001 actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt cagaatattg
     9061 agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc actcaaacag
     9121 agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc cagattttgc
     9181 gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat ataaatacag
     9241 ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa cgaacatcat
     9301 caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg gtcagcaaga
     9361 caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt attaacgcgc
     9421 gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga ggagatcctc
     9481 gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt actctcccgc
     9541 tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat tgcggcaggg
     9601 cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga agcatttaag
     9661 caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga acataacaaa
     9721 caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt cgccatattt
     9781 cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat ccaccaactg
     9841 aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga actggcggct
     9901 aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt gaattcaaaa
     9961 cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac cattcaggaa
    10021 cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcac gcagttggaa
    10081 aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt tgttgattga
    10141 gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa acattgcacc
    10201 tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca gacaagaagt
    10261 cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa gttatcaatt
    10321 tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac cctgccagac
    10381 agatttttag ggagagaacc atgctgttac acattttgta tttggttggc attactgccg
    10441 aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt ggcgtaatta
    10501 ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact
    10561 atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac
    10621 tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg
    10681 cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg
    10741 gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc gttttgcgcg
    10801 atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc ggtgtctcat
    10861 ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga
    10921 ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt cttaagctgg
    10981 gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct
    11041 gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct
    11101 gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc gtgctaaata
    11161 aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt agcggtattc
    11221 ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac tgatgccata
    11281 actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata
    11341 aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc caaccggcct
    11401 caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag cgtgaaatga
    11461 actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag cggttttctg
    11521 gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac
    11581 cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc
    11641 gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga
    11701 gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt
    11761 cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc tggcccggta
    11821 gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg gtgacgaagg
    11881 gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca tttttaaccg
    11941 cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg
    12001 tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt
    12061 cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca
    12121 tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg
    12181 ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc tgcgtccagt
    12241 cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa
    12301 ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca
    12361 tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg gcaaccggat
    12421 gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc acaaagcagc
    12481 gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt
    12541 ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt
    12601 cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc
    12661 acaagatact tattaatatc gccatccata ctttcatctt cacctccgct gtagtcaggc
    12721 tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt ctgcgaggac
    12781 gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa cagctacgca
    12841 aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg ccgcgccgtg
    12901 tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct
    12961 caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag cctgattcag
    13021 gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc
    13081 caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt
    13141 aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat
    13201 ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat
    13261 atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt
    13321 attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc
    13381 gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa
    13441 tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt
    13501 attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc
    13561 aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg
    13621 agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt
    13681 tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat
    13741 ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa
    13801 ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagctggaa
    13861 gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg
    13921 tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac
    13981 caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca
    14041 cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat tctggccgag
    14101 atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagatact
    14161 cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag
    14221 ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac
    14281 tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc
    14341 gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc
    14401 gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt
    14461 aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc
    14521 aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa aatcctctcg
    14581 gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg tcgcgagaag
    14641 catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg
    14701 gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg
    14761 caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca
    14821 aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt
    14881 gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt tgccgcgcac
    14941 tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg
    15001 atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc
    15061 gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc
    15121 gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt
    15181 catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc
    15241 agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg
    15301 aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag
    15361 cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt
    15421 aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca
    15481 acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg
    15541 aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg
    15601 cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg ccctattcct
    15661 ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc
    15721 tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat
    15781 aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc
    15841 gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat
    15901 acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc tcgtgaccgt
    15961 gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc
    16021 cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc
    16081 gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt
    16141 tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct
    16201 ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg
    16261 aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg catgcagatt
    16321 ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta cactcgcccg
    16381 acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg
    16441 gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc
    16501 tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt
    16561 gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg
    16621 gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc
    16681 gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga
    16741 aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta
    16801 gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc
    16861 tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg
    16921 ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga
    16981 cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg
    17041 caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg
    17101 gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc
    17161 cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca
    17221 cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac
    17281 tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc
    17341 accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac
    17401 gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg
    17461 gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa aataaactcc
    17521 tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc
    17581 atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta
    17641 aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcac
    17701 tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc aactggtttg
    17761 caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt
    17821 ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca cacgccatct
    17881 tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa cagcatcgtt
    17941 ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc ttccatccgc
    18001 atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg
    18061 atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg actacggtcg
    18121 ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc tgcataactg
    18181 aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc
    18241 aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc
    18301 tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag
    18361 gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg
    18421 ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc
    18481 gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac aaaacgccgc
    18541 tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga
    18601 ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg
    18661 gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc
    18721 agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc ttgatagaac
    18781 gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg agaagaccgc
    18841 cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat caccagcgca
    18901 ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc catgtgcgac
    18961 gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt tgcaccgagg
    19021 ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc catcatgcgc
    19081 caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag aatcgccagc
    19141 atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc catcgccagg
    19201 aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac gcggtaaaag
    19261 cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca gacgaaggtc
    19321 ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac cgtcaggcag
    19381 atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg tcccacatcc
    19441 ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg cgtggtggag
    19501 tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac cagaccgaac
    19561 gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc aatgaacaat
    19621 ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc ggccaacagc
    19681 cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc attttgcatc
    19741 accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc ggcacgcaaa
    19801 ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa cggatcgcgc
    19861 agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag tagcgccagc
    19921 gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa aaaggagacg
    19981 gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag cgttgctaaa
    20041 gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat actcgtcata
    20101 cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg ctttcctggg
    20161 tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa ataccaaaag
    20221 ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg gcaaacgttt
    20281 gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg ttttcgatgt
    20341 ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa aacagcgaac
    20401 tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc gcctgtcagc
    20461 atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc caggcgctgg
    20521 gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc tccggtgtgg
    20581 catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg tctattcagg
    20641 gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg aaaaccggtg
    20701 gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg gcaagttgca
    20761 ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg ccgctggttt
    20821 ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg acctccattg
    20881 gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat ctgctgctgg
    20941 caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct tacttacgcg
    21001 tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt atgggcatgt
    21061 tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg ccgctctatt
    21121 acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt atgatcactt
    21181 cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag ccagtgtccg
    21241 gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg aactcgtttg
    21301 tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac ggagtgatcc
    21361 agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg ttgatcgtgc
    21421 tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg gttctgggcg
    21481 gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc gtttctcgtg
    21541 agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt ggtctgggcg
    21601 tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac ctgctctcct
    21661 ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc ccaccagaaa
    21721 aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct ctttcaccat
    21781 tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat ttcacgggat
    21841 ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct ctgttagtgg
    21901 tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat atcagcgcat
    21961 gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac cgtttttccg
    22021 cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag cccgcgcccc
    22081 tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa ccacgccatg
    22141 tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc gcgccgctac
    22201 cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg aatagcgaat
    22261 ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca gaagccggta
    22321 aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg ctcaatgatg
    22381 ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt acgctgacta
    22441 acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt aacgccgacg
    22501 gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc gaaaaatcgc
    22561 taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt cgcttggaag
    22621 tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat ctcagcttgc
    22681 gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg tcgatgaacg
    22741 ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac gcggaatttt
    22801 ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt atggtcagaa
    22861 cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg gcaattgccg
    22921 ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg acacccggtt
    22981 ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc attgatatcg
    23041 accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac ctgacgctgg
    23101 ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc gccgccgcga
    23161 cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac agcagcacgg
    23221 tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc accgcaagtg
    23281 tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt gtgccggtga
    23341 atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc aatattcagc
    23401 ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt agcgggcaat
    23461 tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg ggcgtcgttt
    23521 ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg ccgggtacga
    23581 ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg cattcccttg
    23641 aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca gaccatttac
    23701 tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac cagtcacagt
    23761 aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt cagcagcagg
    23821 cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg ccgtgccttc
    23881 gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag tgttatcacg
    23941 cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac taccgtcgag
    24001 ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact gcacatcgcc
    24061 cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt gacggtcaag
    24121 gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca tcggcgtacc
    24181 ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc atttcagttg
    24241 cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca cacgataaga
    24301 tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc agcgtttgta
    24361 aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga agccaaaacc
    24421 tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt agttagcgta
    24481 acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg agcgggcaaa
    24541 caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca gttcgttgta
    24601 gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc actgaacatc
    24661 aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg cgaccagacc
    24721 tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat aaatcgccag
    24781 acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga gtaaatagcc
    24841 tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt taatccagac
    24901 gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt cagggaaagt
    24961 cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac agtcaaagtg
    25021 gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt ttaccgtcgc
    25081 ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc acgggggcgg
    25141 cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg gcgtcgggcc
    25201 gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt tggagacttt
    25261 ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc cataaccacg
    25321 gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc cgccgtcccg
    25381 gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct ctcccagacc
    25441 gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc gttgattatt
    25501 cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt cgccgttgcg
    25561 cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt tgccactttt
    25621 aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca aaatattgag
    25681 cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca caccgacaat
    25741 accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct gccaggtacg
    25801 ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct gttcaacctc
    25861 gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt ttccatcgct
    25921 aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt gctggcaacg
    25981 gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg tgagtgagta
    26041 gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa tcgtgaatag
    26101 cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat catatccgcc
    26161 ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt tgagcacaaa
    26221 cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact cgccgtagtc
    26281 gacgatatcg aacatcatta cccactggat agatgtcacc agttgatgca acacaccaat
    26341 cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga acatcgcgag
    26401 gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac atttccagtc
    26461 ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag tggtgagaaa
    26521 agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca tcgccccacc
    26581 gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc gccactggtc
    26641 gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg gtgcttcaac
    26701 gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca cggaaaggac
    26761 cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat taaccagtgg
    26821 catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac gccaggattg
    26881 cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc agtaagggat
    26941 attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca tagatcattt
    27001 tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc ccgaatggca
    27061 gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt cgatcggcca
    27121 acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa aacatggttc
    27181 cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata tataacatta
    27241 cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt ttctctttaa
    27301 cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc tgagaccgat
    27361 aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt tattaattta
    27421 tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca ttgtaaaata
    27481 tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt aggcggaaaa
    27541 aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc gcctccatgt
    27601 agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc aaatccagtt
    27661 ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag tttacctctt
    27721 ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt atttatttgt
    27781 atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac tttagggggt
    27841 gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata cataacaacg
    27901 gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt tagttactgg
    27961 cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat ggcattaacc
    28021 gatatcaaag tgaaaaccgc aaaacccaaa gacaaaccct ataagcttgc tgatggcggc
    28081 ggtatgtatc tactgattaa cactaatggt tctaaatact ggcgcatgaa gtaccgcttt
    28141 gctggtaaag aaaagatgct ttctattggc gtataccccg atgtaacgct agccgatgct
    28201 cgcgagaaac gcagtgaagc caggaaactc ctcgctgctg gaggcgatcc aggggaggca
    28261 aaaaaggaag aaaaaatcgc gcagcaaatg agtctgaaaa atacgttcga ggctattgct
    28321 cgcgaatggc accagttaaa agctgatcgg tggtcattac gctatcgtga tgaaatcatt
    28381 gatacctttg aaaaagacat ttttccctac attggcaagc gccctattgc cgaaatcaaa
    28441 ccaatgaagt tactggaagc actgcgtaaa atggaaaaac gtggagctct ggagaaaatg
    28501 cgcaaggtcc gccagcgctg tggtgaggtg ttccgctatg caattgttac tggccgggca
    28561 gactataacc cagcccccga tcttgccagt gctttagcta caccgaaaaa agtacatttt
    28621 ccctttctta ctgctaatga acttccccac ttcctcaacg atctggcggg ttataccgga
    28681 agtataatca ctaagacagc tactcagatc attatgttaa ccggcgtacg aacacaagaa
    28741 ctgcgttttg cacgctggga agatatcgat tttgagacaa aattgtggga gatcccggca
    28801 gaagtaatga agatgaagcg acctcatatt gtgccgctct ctgagcaggt cattatgcta
    28861 tttaagcagc ttgaaccaat ctcaaaacat catcccctag tctttatcgg tagaaatgat
    28921 ccccgcaaac caattagtaa ggaaagcatt aaccaagtaa ttgaattact tggatataag
    28981 ggaagactca cagggcacgg tttcagacac accatgagta caattctgca tgaacaaggc
    29041 tttaattccg cctggattga aatgcagtta gctcatgtgg ataaaaattc catcaggggt
    29101 acctataatc atgccctgta tctcgatggc cgccgtgaaa tgatgcaatg gtacgctgat
    29161 tacattgatt cactctccag ccgggaaagt taagtcagaa atacttttcc ctgatgtttt
    29221 cctcaattaa gtattttatc ctctggccta caaggctgga ggatttttgt tattatatta
    29281 tttttttgcg tcttcaaact tattacaaaa acataattaa aaacaaattg acatttttaa
    29341 attggcaatt acaaaataca gccttgcagt tatttcgata cctttagcaa aggaatagtt
    29401 gattcagata taatttagca tttcaatcat ctgacatcat tataaaaaat gagattcaca
    29461 taaagataaa attattgttc tcaaggaaag catgaagggg cttcgtaagc aatattcatt
    29521 cggtgccaag acaggacttg atacacagaa tctcaacttt tatgctctca gactatatca
    29581 ccacatgttt tacaggctca gaaaatgaac gatagattat gctttgaagt gcacgataac
    29641 aagggttatt tcgtatttcc ggatacctgg tttggcccat tgctggggga gtttgaagaa
    29701 gtgcttgatg catacgacgc tgatgaaatc agcgagacga gttacatcaa caaactccga
    29761 cgccttgctc aacgggagcc ggactttatt gatatacacg ctcatctggc atatgcattt
    29821 ctggaacaga atgccccgcg taaggcactg aacgccgctc ttaaaggact ggcggcaggt
    29881 aatcgtatca tcccggaaag tttctgtggt gaaataatct ggatgcatcc cgaaaaccgg
    29941 ccttatctgc gcgcgcttta tgcagcaatt ctcgcaaatg tgcacttaca gcgccatcag
    30001 gatgcggtta tgctcactga taaaatactg gcatacaatc ctgaagataa tcagggggct
    30061 cgctggttgt tgggttcaga gctgttacgc accggcgatc atgagcgagc cttcagtgta
    30121 ctgaaagagc atgctgatga attttcacct tactggtatg aactcggtct cctgcatttt
    30181 ctgaacgggg aacatgttaa ggccgccaca gcttttcgcc atggctttgc cacaaacact
    30241 tacatcgcag aaatgctgtg cggaaatctg catccatttc cacttgctgt gtggcatgac
    30301 ttctccggct ctctggatac ggcagaagat tactatgcaa catactcacc attatgggga
    30361 caatatccgg aagcattgct gtttgtaaac tggctctata accattcctc cgtgctgcat
    30421 gaacgatcag aaatcattaa atgcgccgaa atgctgattc aggaagatga tttcgaaata
    30481 tgcgaaagta ttttaagaca gcaggagcac ctgtggaaaa gaattgacaa gacactttct
    30541 gaggaaattg tacagaaatg cagaaatatg aacggtgaat atatctggcc ctggatattg
    30601 cccttttccg cagcagggat gaaacattca agcatccagt atcagtagat attgcattag
    30661 tgtaacttta aaacaagtaa taatttatta tccaataata acactgtgtt ccttccccag
    30721 gtgattaccc tggggttatt ttctgaaagt ttcctttgca aaaggatagc gttgtaaatt
    30781 actttaacga attactccat gaactcatta aagttctttc gtaatacttt atcgtcaaac
    30841 acgttatagc tggcatcaaa aaccacagat ttaccctgcc agcgggtaat cccctcctct
    30901 ggcgttttcg gttgaactgg tatccccgtc caacacaggc agccattata aacagtaaaa
    30961 aattgtattc gctgttatga aatcaatagt cctgacgtac tgttgaatgt gcttgtgttc
    31021 tgaacctcgt acaaccagta catttgatat cgaattataa acaaaacatt gcatccaact
    31081 tttttaatat ggacaatagt tgtccatacg gtggttttat attcgataaa tcaatggatt
    31141 tcattgagtc acaggactgg taatggatga aaatgcttta aggtttgcct catactggcg
    31201 caactcgctt gcagatgctg agtcaggaaa gggctgtttt gaacggaaag acgcccaaaa
    31261 tttcactcac tggcatggga tagcggcggg acgtcttgac gaagcgattg tcaataaatt
    31321 ttttaaggga gaaaaagacg atgtcgaaac tgtcaatgtc atcttacgcc caaaagttta
    31381 tttccggtta ctgcagcatg gtaaggaccg ttccgcaggc acgcctgata ttgttacacc
    31441 gatagtgacg ccagccttgc taagccgtga gggtttttta tatccgacgc cagcgacctc
    31501 cattcccaga gacctgcttg aacctttgcc aaaaggagca ttttcgattg gtgagattgg
    31561 gcagtatgac aaatacaaga cgacccatac tgatcctacc cacgtaatgt ggacacggcc
    31621 ctaagcgaga ttatggtttt caaattgttc cgggctgaga ccgccacagg cactgtgacg
    31681 acgccagcga ttgtaatcac actcgatata attaaacacc gttgcccgca ttatttcccg
    31741 gctgctaaag cgttccccgt ggatacattc caccttcagc gaatgaaaga agctttccac
    31801 acaggcattg tcataacagt agcctttcgc actcatactg ccacgcaggt tgtgtcgctt
    31861 cagcattgcc tgataatccc ctgaacagta ttgaccacca cggtccgtat gaacaatgac
    31921 gctttccggg cgtcttctcc gccacaacgc catttgcagt gcatcacagg ccagttgtgc
    31981 tgtcattcgc ggtgacatcg accagccaat gacggcgcgt gaccacaggt cgatgactac
    32041 tgcgagatac aaccagccct catcggtacg caagtacgtg atgtcacccg cccacttctg
    32101 gtttcgggcc actggcgctg aagtcctgct ccagcagatt ctccaatacg ggcaggccat
    32161 gtgcacggta gctgaccggg ctgaacttcc ggctggcttt cgcccgcagc ccctgacgac
    32221 gcaggctggc ggcaatggtt ttaatattga actccggcag ttcgtcagca aggcggggag
    32281 caccgtatcg ctgctttgcc tcaaagaatg ccttatggac agcggcatcg caggtgagcc
    32341 gaaactgttg gcgcaggctc atctggtgac gacgcctgag ccagacatac cagccgctgc
    32401 gggcaacccg aagtacacga cacatcgctt tgatgctgaa ctctgcccga tgattttcga
    32461 taaagacata cttcatttca ggcgcttcgc gaagtatgtc gcggcctttt ggaggatgga
    32521 cagttcctca gcctgctccg ccagttgtcg tttaaggcgg acattttcag cggccagttc
    32581 gctttcgcgc tctgacgaac tcatttgttg ctgctgttta ctgcgccagg cataaagctg
    32641 agattcatac aggctgagtt cacgggctgc ggcggccaca ccgatgcgtt cagcgagttt
    32701 cagggcttcg ttacgaaatt caggcgtatg ttgtttacgg ggcttcttgc tgattgatac
    32761 tggttttgtc atgagtcacc tctggttgag agtttactca cttagtcctg tgtccactat
    32821 tggtgggtaa gatcaccctc cattaaaact aaaacgggcg ctttctgata tatcatctgt
    32881 aagcgcagta ataccattag tagcacctct cggcggcgca ttgattattg aatattataa
    32941 ctggaatgtt atatttcttg ttatgatgct gttcagtatt ataataatgt tattatcacc
    33001 aaagataact catgcaaatg agaaagtaat atgtcaaaac agtgcatcct ttgtgaccaa
    33061 agagtttatc tgcggcacta tacttgtctc attaatactc agttctctat tttgctacat
    33121 atcaatatca ccatcaattt tcatgcatac ttatggcctg agtactttta gttactcaat
    33181 tattttctcc ttctcagttt tatttttcat aatcggaaat caattatcaa agattgaaaa
    33241 attatccaac ctatggtttt tattacccaa tagccaattt tatcgcatta caaataaatg
    33301 gttcaaaaac aggatttgcc gcatctatta
//
LOCUS       DAJVPP010000039        33318 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVPP010000039 DAJVPP010000000
VERSION     DAJVPP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.039"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            14..1471
                     /gene="ravA"
                     /locus_tag="OQM34_001802"
     CDS             14..1471
                     /gene="ravA"
                     /locus_tag="OQM34_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1452282.1"
                     /translation="MSSSLEKGLYERSHAIRLCLLAALSGESVFLLGPPGIAKSLIAR
                     RLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTSGYLPEAEIVFLDE
                     IWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEADSSLEALYDRMLI
                     RLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKEIGEITLPDHVFEL
                     IFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVDLILLKDCLWYDAQ
                     SLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKTALTLIRLGGIFSR
                     RQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLSKGGEIRGKLNGIG
                     FAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQLEELESDWRKQHAL
                     FSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1465..2916
                     /gene="viaA"
                     /locus_tag="OQM34_001803"
     CDS             1465..2916
                     /gene="viaA"
                     /locus_tag="OQM34_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS1452283.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(2921..3913)
                     /gene="asnA"
                     /locus_tag="OQM34_001804"
     CDS             complement(2921..3913)
                     /gene="asnA"
                     /locus_tag="OQM34_001804"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1452284.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4065..4523
                     /gene="asnC"
                     /locus_tag="OQM34_001805"
     CDS             4065..4523
                     /gene="asnC"
                     /locus_tag="OQM34_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1452285.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4613..5056
                     /gene="mioC"
                     /locus_tag="OQM34_001806"
     CDS             4613..5056
                     /gene="mioC"
                     /locus_tag="OQM34_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1452286.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5435..7324
                     /gene="mnmG"
                     /locus_tag="OQM34_001807"
     CDS             5435..7324
                     /gene="mnmG"
                     /locus_tag="OQM34_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1452287.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7388..8011
                     /gene="rsmG"
                     /locus_tag="OQM34_001808"
     CDS             7388..8011
                     /gene="rsmG"
                     /locus_tag="OQM34_001808"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1452288.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8628..9008
                     /gene="atpI"
                     /locus_tag="OQM34_001809"
     CDS             8628..9008
                     /gene="atpI"
                     /locus_tag="OQM34_001809"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1452289.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9017..9832
                     /gene="atpB"
                     /locus_tag="OQM34_001810"
     CDS             9017..9832
                     /gene="atpB"
                     /locus_tag="OQM34_001810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1452290.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9879..10118
                     /gene="atpE"
                     /locus_tag="OQM34_001811"
     CDS             9879..10118
                     /gene="atpE"
                     /locus_tag="OQM34_001811"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1452291.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10180..10650
                     /gene="atpF"
                     /locus_tag="OQM34_001812"
     CDS             10180..10650
                     /gene="atpF"
                     /locus_tag="OQM34_001812"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1452292.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10665..11198
                     /gene="atpH"
                     /locus_tag="OQM34_001813"
     CDS             10665..11198
                     /gene="atpH"
                     /locus_tag="OQM34_001813"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1452293.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11211..12752
                     /gene="atpA"
                     /locus_tag="OQM34_001814"
     CDS             11211..12752
                     /gene="atpA"
                     /locus_tag="OQM34_001814"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1452294.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12803..13666
                     /gene="atpG"
                     /locus_tag="OQM34_001815"
     CDS             12803..13666
                     /gene="atpG"
                     /locus_tag="OQM34_001815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1452295.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13693..15075
                     /gene="atpD"
                     /locus_tag="OQM34_001816"
     CDS             13693..15075
                     /gene="atpD"
                     /locus_tag="OQM34_001816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1452296.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15096..15515
                     /gene="atpC"
                     /locus_tag="OQM34_001817"
     CDS             15096..15515
                     /gene="atpC"
                     /locus_tag="OQM34_001817"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1452297.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15866..17236
                     /gene="glmU"
                     /locus_tag="OQM34_001818"
     CDS             15866..17236
                     /gene="glmU"
                     /locus_tag="OQM34_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1452298.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17398..19227
                     /gene="glmS"
                     /locus_tag="OQM34_001819"
     CDS             17398..19227
                     /gene="glmS"
                     /locus_tag="OQM34_001819"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1452299.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19387..20208
                     /locus_tag="OQM34_001820"
     CDS             19387..20208
                     /locus_tag="OQM34_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCS1452300.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20195..22303
                     /locus_tag="OQM34_001821"
     CDS             20195..22303
                     /locus_tag="OQM34_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS1452301.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22300..23967
                     /locus_tag="OQM34_001822"
     CDS             22300..23967
                     /locus_tag="OQM34_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1452302.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            23970..25496
                     /locus_tag="OQM34_001823"
     CDS             23970..25496
                     /locus_tag="OQM34_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCS1452303.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25497..27113
                     /locus_tag="OQM34_001824"
     CDS             25497..27113
                     /locus_tag="OQM34_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS1452304.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27169..27321)
                     /locus_tag="OQM34_001825"
                     /pseudo
     CDS             complement(27169..27321)
                     /locus_tag="OQM34_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27344..27721)
                     /locus_tag="OQM34_001826"
     CDS             complement(27344..27721)
                     /locus_tag="OQM34_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452305.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28131..28502)
                     /locus_tag="OQM34_001827"
     CDS             complement(28131..28502)
                     /locus_tag="OQM34_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452306.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28563..29060)
                     /locus_tag="OQM34_001828"
     CDS             complement(28563..29060)
                     /locus_tag="OQM34_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452307.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29136..29924)
                     /gene="aadA1"
                     /locus_tag="OQM34_001829"
     CDS             complement(29136..29924)
                     /gene="aadA1"
                     /locus_tag="OQM34_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1452308.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(29982..30506)
                     /gene="sat2"
                     /locus_tag="OQM34_001830"
     CDS             complement(29982..30506)
                     /gene="sat2"
                     /locus_tag="OQM34_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1452309.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(30601..31074)
                     /locus_tag="OQM34_001831"
     CDS             complement(30601..31074)
                     /locus_tag="OQM34_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1452310.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            31406..31942
                     /locus_tag="OQM34_001832"
     CDS             31406..31942
                     /locus_tag="OQM34_001832"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCS1452311.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            32057..32383
                     /locus_tag="OQM34_001833"
     CDS             32057..32383
                     /locus_tag="OQM34_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1452312.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(32571..32810)
                     /locus_tag="OQM34_001834"
     CDS             complement(32571..32810)
                     /locus_tag="OQM34_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS1452313.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         8640 a         7770 c         8427 g         8481 t
ORIGIN      
        1 aagaatttcc cgcctgagca gttcgttgga aaaggggctt tatgaacgta gccacgccat
       61 ccgcttgtgt ttattagcgg cattaagtgg tgaaagtgtg ttcctccttg gcccgccagg
      121 tattgccaaa agtttgatcg cccggcgctt aaaattcgcc tttcagaatg cccgcgcgtt
      181 tgaatatctg atgacccgct tctccacgcc ggaagaagtt tttggtcccc tttctattca
      241 ggcgctaaaa gatgaagggc gctatgaacg tttaaccagc ggttacctgc cggaagcaga
      301 aatcgtcttt ctggatgaga tctggaaagc gggcccggca attcttaata ccttgctcac
      361 cgccattaac gagcgccagt tccgcaacgg tgcacacgta gaaaaaatcc cgatgcgtct
      421 actggtggcg gcctccaacg agctgccgga agcagacagc agtctggaag cgttatatga
      481 ccgcatgctg attcgtctat ggttagataa agtgcaagat aaagcgaatt tccgctccat
      541 gctgaccagt caacaggatg aaaacgacaa tccggttcct gatgccttgc aggtcacaga
      601 tgaagaatat gaacgctggc agaaagagat tggtgaaatt acgctgcccg atcatgtatt
      661 tgagctgatt tttatgctgc gccagcaact ggataaatta ccggatgcgc cttatgtctc
      721 ggatcgtcgc tggaaaaaag cgatccgctt attgcaggcc agcgcctttt ttagcggtcg
      781 cagtgctgtt gccccggttg atctcatttt gctgaaagat tgcctgtggt atgacgcgca
      841 aagcctgaat ttgatacaac aacaaattga tgtattgatg accggtcatg cctggcaaca
      901 gcaagggatg ttgacccgcc tgggcgcgat tgtgcaacgt cacctgcaac tccagcagca
      961 acaaagcgat aaaacagcct taacgttaat tcgtctgggc ggcattttca gccgtcgtca
     1021 gcagtatcaa cttcctgtta acgttactgc ttccactctg actctgctgc tgcaaaaacc
     1081 gttaaaactg catgatatgg aagtggttca tatctccttt gagcgtagcg cgctggaaca
     1141 gtggctgagt aaaggtggtg aaattcgcgg caaactgaac ggtatcggct ttgcccagaa
     1201 actgaatctg gaagttgata gcacccaaca tcttgttgta cgcgatgtaa gtttacaagg
     1261 cagtacgctg gcacttcccg gttcatcggc tgaaggtctg ccaggtgaaa taaaacaaca
     1321 actggaagag cttgaaagcg actggcgtaa gcaacacgct ttattcagcg aacagcaaaa
     1381 atgtctgttt atccctggcg actggttagg tcgcattgaa gacagcctac aggatgtcgg
     1441 tgcacagatt cgtcaggcac aacaatgcta acgctggata cgcttaatgt gatgctggcc
     1501 gtcagcgaag agggattgat cgaagagatg atcatcgcac tgctggcctc accgcagctg
     1561 gcggtcttct ttgaaaaatt cccacgactg aaggcggcaa tcactgatga tgttccccgc
     1621 tggcgtgagg cgctgcgcag tcggctgaaa gatgcccaag tcccgccgga actcaccgaa
     1681 gaggtgatgt gctatcagca aagccagctc ctctccacgc cgcagtttat tgtgcagcta
     1741 ccacagatcc tggacttact gcatcgtctg aattccccat gggcagaaca agcccggcag
     1801 ttggttgatg ctaacagcac aataacttca gcgttacaca cgctttttct ccagcgttgg
     1861 cgtttaagtc tgatcgtgca agcaacgacg ttaaatcaac agctattaga agaagaacgc
     1921 gaacaactgt taagtgaagt tcaggaacgc atgacgctga gcggacaact tgaaccgatt
     1981 ctcgcagata acaataccgc agctggtcgt ctgtgggata tgagcgccgg tcaacttaaa
     2041 cgtggcgact atcagtttat tgtgaaatac ggtgaatttc ttaacgaaca gccggaactg
     2101 aaacgcctgg cagagcagct ggggcgttct cgggaagcca aatcaatacc gcgcaacgat
     2161 gcgcagatgg aaaccttccg caccatggtg cgcgaaccgg cgacggttcc tgagcaggtt
     2221 gatggtctgc aacaaagcga tgatatttta cgtctcctgc cgccagaact ggcgacacta
     2281 gggataacgg aactggagta tgagttttac cgtcggctgg tggaaaaaca gttgctcacc
     2341 tatcgcctgc acggtgagtc gtggcgtgaa aaagtgctcg aacgtccggt ggtacataaa
     2401 gattacgatg aacagccgcg cgggccgttt attgtctgtg tggatacttc cggctcaatg
     2461 ggcggcttta atgaacagtg tgcgaaagcg ttctgcctgg ctttgatgcg cattgctctc
     2521 gctgaaaacc ggcgctgtta tattatgcta ttttccaccg agatcgtccg ttatgagctt
     2581 tcaggcccac aaggcatcga acaagcaatc cgttttttaa gccagcagtt tcgtggcggc
     2641 accgatcttg ccagttgttt tcgcgccatt atggaacgct tgcaaagcag ggaatggttt
     2701 gatgccgatg cggtggtgat ttctgatttt atcgctcagc ggttgcctga cgacgtgacg
     2761 agtaaagtga aagagctgca gcgggtacat cagcatcgct ttcatgccgt ggcgatgtcg
     2821 gcacacggca aacccggcat catgcgcatt ttcgatcata tctggcgctt tgataccggg
     2881 atgcgaagcc gcctgctcag acgctggcgg cgataaatta ttacagcaga gaagggacgc
     2941 tctcgcgaac agcagctggc catactccac actgaacctg gccgatatgc ggcagttgca
     3001 gcagcagcat agtcaaacga gactggccga taccgccgcc gatggtctgc ggcatttcac
     3061 cgcgcagcag cgcctgatgc cattccagct gcagacgatc ttcatcaccg gtcagcgcca
     3121 gctgatgctt cagcgtgtcg gcatccacgc ggatccccat ggaagaaagc tcaaacgcat
     3181 cttccagtac cgggttccac accagaatat cgccgttcag gcccgcatgg cccagctctg
     3241 acggggtgct ccagtcatca taatccggtg cgcgcacgtc gtgacgatga ccatcactca
     3301 acttgccgcc aatcccgacg aggaataccg cgccaagatc tttcgcaatc gcccgctcac
     3361 gccctttggc atcgagatcc ggataacgag acagtaactc ctggctgtgt acgaagtgaa
     3421 tctgatccgg caggaacggt gccaggccaa actcttcgtt aaccgcagct tcggttgctt
     3481 taattcccgc ccagatcgcc tctaccgtgc ttttcagagt cgagagttga cgctcaccgt
     3541 cgcccattac gcgttcccag tcccactggt caacatagac cgagtgcaac ggagaaagac
     3601 ggtcttcatc ggggcgaagg gctttcatgt gcgtgtacag cccttcgccc gcgctgaagt
     3661 cgtgttgccc taaggtctga cgtttccact tcgccagtga atgaaccact tcgaactggg
     3721 catcaggcag agctttcact tttacctgca ccgctttttc acagcccgac aagttatcct
     3781 gcgtgccatc ccccacacgg ctaagaatcg gcgcctggac ttcgatcagc cccagacgtt
     3841 cttccagttg gcgagaaaag tgagatttca cgaagctaat ttggcgttgt ttggcaatgt
     3901 aagcggtttt cattttttat actcctgcgt cctgttgctt atgattaagc aacaaaaacc
     3961 gacacacatg caataatcat tcaataaaaa gcctaacaca cttttgattc attaaaaaag
     4021 acggctaaaa tagaatgaat catcaatctg cataagaaaa tcctatggaa aattatctga
     4081 tcgacaatct ggaccgtggc atcctggaag cattaatggg caatgcgcgc accgcttacg
     4141 ccgaactggc gaaacaattt ggcgtcagtc cggggacgat tcacgttcga gtagagaaaa
     4201 tgaagcaggc ggggatcatt accggggcgc gtattgatgt cagcccgaag cagctcggtt
     4261 atgacgtagg ctgctttatc ggcattatat taaagagcgc caaagactac ccttccgcgc
     4321 tggcaaagct ggaaagcctt gatgaagtca ctgaagccta ttacacaacc ggccactaca
     4381 gcatctttat aaaagttatg tgccgttcga tcgacgctct ccagcatgta cttatcaaca
     4441 agatccaaac aattgatgaa attcagtcca ccgagacatt gatcgtcctg caaaacccga
     4501 tcatgcgtac catcaagccc tgatcggctt ttttaatccc atacttttcc acaggtagat
     4561 cccaacgcgt tcacagcgta caatacgcca ctcttaataa aggtggcggt ttatggcaga
     4621 tatcactctt atcagcggca gcaccctcgg cggtgccgaa tatgtagcag aacacctggc
     4681 tgaaaagctg gaagaggtgg gttttaccac cgaaacgctg cacggtccgc tgttggaaga
     4741 tttatctgcc tcggggatct ggctggttat cagctccacc cacggtgccg gagatattcc
     4801 ggacaacctt tctcctttct atgaagcatt gcaggaacag aagcccgatc tttctgcggt
     4861 ccgctttggc gcaatcggta ttggcagtcg tgaatatgac accttttgtg gggctatcga
     4921 taaactcgag gcagaactca aaaattccgg tgcaaaacag acaggcgaaa cactgaagat
     4981 caacattctt gatcacgaca ttccggaaga tccggcagaa gaatggctgg gatcgtggat
     5041 taatttactc aaataagtat acagatcgtg cgatctactg tggataactc tgtcaggaag
     5101 cttggatcaa ccggtagcta tccaaagaac aaccgttgtt cagtttttga gttgtgtata
     5161 acccctcatt ctgatcccag cttatacggt ccaggatcac cgatcattca cagttaatga
     5221 tcctttccag gttgttgatc ttaaaaatcg gatccttgtt atccacaggg cagtgcgatc
     5281 ctaataagag atcacaatag aacagatctc taaataaata gatcttcttt ttaataccca
     5341 ggatcccagg tctttctcaa gccgacaaag ttgagtagaa tccacggccc gggcttcaat
     5401 ccattttcat accgcgttat gcgaggcaat caccatgttt tatccggatc cttttgacgt
     5461 catcatcatt ggcgggggtc atgcaggcac cgaggccgcg atggctgcgg cgcgtatggg
     5521 tcaacagact ctgcttttga cacacaatat cgacactctg gggcagatga gctgcaaccc
     5581 ggcgatcggc ggtattggga agggacatct ggtaaaagaa gtggatgcac tcggcggtct
     5641 gatggcgaaa gcgatcgatc aagcgggtat ccagtttagg atactaaacg caagcaaagg
     5701 accggcggtt cgcgctaccc gagctcaggc ggatcgtgtg cgctaccgtc aggcggtacg
     5761 tacggcgctg gagaaccaac cgaacctgat gatcttccag caggcggttg aagatcttat
     5821 tgtcgaaaac gatcgcgtgg tcggtgctgt tacccaaatg ggactgaagt tccgtgccaa
     5881 agctgtggtg ctcaccgttg ggacgttcct cgacggcaaa attcatatcg gtctggataa
     5941 ttacagcggt ggccgtgctg gcgatccgcc atccattccg ctctctcgcc gtttgcgtga
     6001 actgccgctg cgcgttggtc gtctgaaaac cggtactccg ccgcgtattg atgctcgaac
     6061 catcgacttt agcgtgctgg cgcaacagca tggcgataac ccaatgccgg tattctcgtt
     6121 tatgggcaat gcgtccctgc atccccagca ggtgccgtgt tatatcactc ataccaacga
     6181 gaaaacccat gatgtgatcc gcagtaacct cgatcgtagc ccaatgtatg caggggtgat
     6241 cgaaggtgtc ggcccacgct actgcccgtc gatcgaagac aaagtcatgc gcttcgcaga
     6301 cagaaatcag catcagatct tccttgaacc ggaaggactg acctctaatg aaatttatcc
     6361 gaacggtatc tccaccagcc tgccgttcga tgtgcagatg caaatcgtcc gctctatgca
     6421 ggggatggaa aacgcgaaga tcgtgcgtcc gggttatgcc attgagtatg acttcttcga
     6481 tcctcgcgac ctgaaaccga cgctggagag caagtttatc caggggctgt tctttgctgg
     6541 tcagattaac ggcactaccg gttacgaaga agccgctgcg caaggtttgc tggccggtct
     6601 taacgctgcc cgtctgtctg ctgacaaaga aggttgggct ccggcacgtt ctcaggcgta
     6661 tctcggcgta ctggttgatg acctgtgcac tttaggaacc aaagaaccgt atcgtatgtt
     6721 tacttcgcgc gcagaatatc gtctgatgct gcgcgaagat aatgcggatc tgcgtttgac
     6781 tgaaatcggt cgtgaactgg gcctggtgga tgacgaacgt tgggcgcgct ttaacgagaa
     6841 acttgagaat atcgagcgtg agcgtcagcg tctgaaatcg acctgggtaa ccccgtcggc
     6901 ggaagctgca gccgaagtga atgctcacct gactgcgccg ctttcccgtg aagccagtgg
     6961 tgaagatctg ctgcgtcgtc cggaaatgac ttatgaaaaa ttaaccacgc tgacgccgtt
     7021 tgcccctgcg ttgacagacg aacaggcggc ggaacaggtt gagattcagg ttaaatacga
     7081 aggttatatc gcgcgccagc aagatgagat cgaaaagcag ctgcgtaacg agaacaccct
     7141 gctacccgcg acactggatt accgccagat atccggtctt tctaacgaag tgatcgccaa
     7201 acttaacgat cacaaaccgg cctctatcgg ccaggcttcg cgtatttctg gcgtcacgcc
     7261 tgcggccatc tccattctgc tggtgtggct gaaaaaacag ggtatgctgc gtcgtagcgc
     7321 ataacgcatt aaaaatgcct ggtaagcacc cgcttaccag gcaacgcatc aagaacaggt
     7381 aatcaccgtg ctcaacaaac tctccttact gctgaaagac gcaggtattt cgcttactga
     7441 tcaccagaaa aaccagctta ttgcctacgt gaatatgctg cataaatgga acaaagcgta
     7501 caacctgact tcggtccgcg atcctaatga gatgctggta cgccatattc tcgatagcat
     7561 tgtggtggca ccgtatctgc aaggtgaacg gtttatcgat gtcggcaccg gaccaggact
     7621 gccaggcatt ccactctcta tcgtgcgtcc tgaagcccat ttcactctgt tggatagcct
     7681 tggtaaacgc gtgcgtttcc ttcgtcaggt gcaacatgag cttaaactgg agaatattga
     7741 accagtacag agcagggtag aagagtttcc ttcagagccg ccatttgatg gcgtaattag
     7801 ccgcgctttt gcctctctga acgatatggt gagctggtgc caccatcttc ctggtgagca
     7861 aggccgtttc tacgcgctga aagggcaaat gccggaagat gaaatcgctt tgttgcccga
     7921 agaatatcag gtcgaatcag tggttaaact tcaggttcca gccctggatg gcgaacgtca
     7981 tctggtggtg attaaagcaa ataaaattta atttttatca aaaaaatcat aaaaaattga
     8041 ccggttagac tgttaacaac aaccaggttt tctactgata taactggtta catttaacgc
     8101 cacgttcact cttttgcatc aacaagataa cgtggctttt tttggtaagc agaaaataag
     8161 tcataagtga aaatatcagt ctgctaaaaa tcggcgctaa gaaccatcat tggctgttaa
     8221 aacattatta aaaatgtcaa tgggtggttt ttgttgtgta aatgttattt attaaaacag
     8281 tatctgtttt tagactgaaa tatcataaac ttgtaaaggc atcatttgcc aagtaaataa
     8341 atatgctgtg cgcgaacatg cgcaatatgt gatctgaagc acgctttatc accagtgttt
     8401 acgcgttatt tacagttttt catgatcgaa caggggtagc ggaaaagtcg caattgtatg
     8461 cactggaaaa atatttaaac atttattcac cttttggcta cttattgttt gaaatcacgg
     8521 gggcgcaccg tataatttga ccgctttttg atgcttgact ctaagcctta aagaaagttt
     8581 tatacgacac gcggcatacc tcgaagggag caggagtgaa aaacgtgatg tctgtgtcgc
     8641 tcgtgagtcg aaacgttgct cggaagcttc tgctcgttca gttactggtg gtgatagcaa
     8701 gtggattgct gttcagcctc aaagacccct tctggggcgt ctctgcaata agcgggggcc
     8761 tggcagtctt tctgcctaac gttttgttta tgatatttgc ctggcgtcac caggcgcata
     8821 caccagcgaa aggccgggtg gcttggacat tcgcatttgg cgaagctttc aaagttctgg
     8881 cgatgttggt gttactggtg gtggcgttgg cggttttaaa ggcggtattc ttgccgctga
     8941 tcgttacgtg ggttttggtg ctggtggttc agatactggc accggctgta attaacaaca
     9001 aagggtaaaa ggcatcatgg cttcagaaaa tatgacgccg caggattaca taggacacca
     9061 cctgaataac cttcagctgg acctgcgtac attctcgctg gtggatccac aaaacccccc
     9121 agccaccttc tggacaatca atattgactc catgttcttc tcggtggtgc tgggtctgtt
     9181 gttcctggtt ttattccgta gcgtagccaa aaaggcgacc agcggtgtgc caggtaagtt
     9241 tcagaccgcg attgagctgg tgatcggctt tgttaatggt agcgtgaaag acatgtacca
     9301 tggcaaaagc aagctgattg ctccgctggc cctgacgatc ttcgtctggg tattcctgat
     9361 gaatctgatg gatttactgc ctatcgactt gctgccgtac attgctgaac atgtactggg
     9421 tctgcctgca ctgcgtgtgg ttccgtctgc ggacgtgaac gtaacgctgt ctatggcact
     9481 gggcgtattt atcctgattc tgttctacaa catcaaaatg aaaggcatcg gcggcttcac
     9541 gaaagagttg acgctgcagc cgttcaatca ctgggcgttc attcctgtca acttaatcct
     9601 tgaaggggta agcctgctgt ccaaaccagt ttcactcggt ttgcgactgt tcggtaacat
     9661 gtatgccggt gagctgattt tcattctgat tgctggtctg ttgccgtggt ggtcacagtg
     9721 gatcctgaat gtgccgtggg ccattttcca catcctgatc attacgctgc aagccttcat
     9781 cttcatggtt ctgacgatcg tctatctgtc gatggcgtct gaagaacatt aatttaccaa
     9841 cactactacg ttttaactga aacaaactgg agactgtcat ggaaaacctg aatatggatc
     9901 tgctgtacat ggctgccgct gtgatgatgg gtctggcggc aatcggtgct gcgatcggta
     9961 tcggcatcct cgggggtaaa ttcctggaag gcgcagcgcg tcaacctgat ctgattcctc
    10021 tgctgcgtac tcagttcttt atcgttatgg gtctggtgga tgctatcccg atgatcgctg
    10081 taggtctggg tctgtacgtg atgttcgctg tcgcgtagta agcgttgctt ttatttaaag
    10141 agcaatatca gaacgttaac taaatagagg cattgtgctg tgaatcttaa cgcaacaatc
    10201 ctcggccagg ccatcgcgtt tgtcctgttc gttctgttct gcatgaagta cgtatggccg
    10261 ccattaatgg cagccatcga aaaacgtcaa aaagaaattg ctgacggcct tgcttccgca
    10321 gaacgagcac ataaggacct tgaccttgca aaggccagcg cgaccgacca gctgaaaaaa
    10381 gcgaaagcgg aagcccaggt aatcatcgag caggcgaaca aacgccgctc gcagattctg
    10441 gacgaagcga aagctgaggc agaacaggaa cgtactaaaa tcgtggccca ggcgcaggca
    10501 gaaattgaag ccgagcgtaa acgtgcccgt gaagagctgc gtaagcaagt tgctatcctg
    10561 gctgttgctg gcgccgagaa gatcatcgaa cgttccgtgg atgaagctgc taacagcgac
    10621 atcgtggata aacttgtcgc tgaactgtaa ggagggaggg gctgatgtct gaatttatta
    10681 cggtagctcg cccctacgcc aaagcagctt ttgactttgc cgtcgaacac caaagtgtag
    10741 aacgctggca ggacatgctg acgtttgccg ccgaggtaac caaaaacgaa caaatggcag
    10801 agcttctctc tggcgcgctt gcgccagaaa cgctcgccga gtcgtttatc gcagtttgtg
    10861 gtgagcaact ggacgaaaac ggtcagaacc tgattcgggt aatggctgaa aatggtcgtc
    10921 ttaacgcgct cccggatgtt ctggagcagt ttattcacct tcgtgccgtg agtgaggcta
    10981 ccgctgaggt agacgtcatt tccgctgccg cactgagtga acaacagctc gcgaaaattt
    11041 ctgctgcgat ggaaaaacgt ctgtcacgca aagttaagct gaattgcaaa atcgataagt
    11101 ctgtaatggc aggcgttatc atccgagcgg gtgatatggt cattgatggc agcgtacgcg
    11161 gtcgtcttga gcgccttgca gacgtcttgc agtcttaagg ggactggagc atgcaactga
    11221 attccaccga aatcagcgaa ctgatcaagc agcgcattgc tcagttcaat gttgtgagtg
    11281 aagctcacaa cgaaggtact attgtttctg taagtgacgg tgttatccgc attcacggcc
    11341 tggccgattg tatgcagggt gaaatgatct ccctgccggg taaccgttac gctatcgcac
    11401 tgaacctcga gcgcgactct gtaggtgcgg ttgttatggg tccgtacgct gaccttgccg
    11461 aaggcatgaa agttaagtgt actggccgta tcctggaagt tccggttggc cgtggcctgc
    11521 tgggccgtgt ggttaacact ctgggtgcac caatcgacgg taaaggtccg ctggatcacg
    11581 acggcttctc tgctgtagaa gcaatcgctc cgggcgttat cgaacgtcag tccgtagatc
    11641 agccggtaca gaccggttat aaagccgttg actccatgat cccaatcggt cgtggtcagc
    11701 gtgaattgat catcggtgac cgtcagacag gtaaaaccgc actggctatc gatgccatca
    11761 tcaaccagcg cgattccggt atcaaatgta tctatgtcgc tatcggccag aaagcgtcca
    11821 ccatttctaa cgtggtacgt aaactggaag agcacggcgc actggctaac accatcgttg
    11881 tggtagcaac cgcgtctgaa tccgctgcac tgcaatacct ggcaccgtat gccggttgcg
    11941 caatgggcga atacttccgt gaccgcggtg aagatgcgct gatcatttac gatgacctgt
    12001 ctaaacaggc tgttgcttac cgtcagatct ccctgctgct ccgtcgtccg ccaggacgtg
    12061 aagcattccc gggcgacgtt ttctacctcc actctcgtct gctggagcgt gctgcacgtg
    12121 ttaacgccga atacgttgaa gccttcacca aaggtgaagt gaaagggaaa accggttctc
    12181 tgaccgcact gccgattatc gaaactcagg cgggtgacgt ttctgcgttc gttccgacca
    12241 acgtaatctc cattaccgat ggtcagatct tcctggaaac caacctgttc aacgccggta
    12301 ttcgtcctgc ggttaacccg ggtatttccg tatcccgtgt tggtggtgca gcacagacca
    12361 agatcatgaa aaaactgtcc ggtggtatcc gtaccgctct ggcacagtat cgtgaactgg
    12421 cagcgttctc tcagtttgca tccgaccttg acgatgcaac acgtaagcag cttgaccacg
    12481 gtcagaaagt gaccgaactg ctgaaacaga aacagtatgc gccgatgtcc gttgcgcagc
    12541 agtctctggt tctgttcgca gcagaacgtg gttacctggc ggatgttgaa ctgtcgaaaa
    12601 ttggcagctt cgaagccgct ctgctggctt acgtcgaccg tgatcacgct ccgttgatgc
    12661 aagagatcaa ccagaccggt ggctacaacg acgaaatcga aggcaagctg aaaggcatcc
    12721 tcgattcctt caaagcaacc caatcctggt aacgtctggc ggcttgcctt agggcaggcc
    12781 gcaaggcatt gaggagaagc tcatggccgg cgcaaaagag atacgtagta agatcgcaag
    12841 cgtccagaac acgcaaaaga tcactaaagc gatggagatg gtcgccgctt ccaaaatgcg
    12901 taaatcgcag gatcgcatgg cggccagccg tccttatgca gaaactatgc gcaaagtgat
    12961 tggtcacctt gcacacggta atctggaata taagcaccct tacctggaag accgcgacgt
    13021 taaacgcgtg ggctacctgg tggtgtcgac cgaccgtggt ttgtgcggtg gtttgaacat
    13081 taacctgttc aaaaaactgc tggcggaaat gaagacctgg accgacaaag gcgttcaatg
    13141 cgacctcgca atgatcggct cgaaaggcgt gtcgttcttc aactccgtgg gcggcaatgt
    13201 tgttgcccag gtcaccggca tgggggataa cccttccctg tccgaactga tcggtccggt
    13261 aaaagtgatg ttgcaggcct acgacgaagg ccgtctggac aagctttaca ttgtcagcaa
    13321 caaatttatt aacaccatgt ctcaggttcc gaccatcagc cagctgctgc cgttaccggc
    13381 atcagatgat gatgatctga aacataaatc ctgggattac ctgtacgaac ccgatccgaa
    13441 ggcgttgctg gataccctgc tgcgtcgtta tgtcgaatct caggtttatc agggcgtggt
    13501 tgaaaacctg gccagcgagc aggccgcccg tatggtggcg atgaaagccg cgaccgacaa
    13561 tggcggcagc ctgattaaag agctgcagtt ggtatacaac aaagctcgtc aggccagcat
    13621 tactcaggaa ctcaccgaga tcgtctcggg ggccgccgcg gtttaaacag gttatttcgt
    13681 agaggattta agatggctac tggaaagatt gtccaggtaa tcggcgccgt agttgacgtc
    13741 gaattccctc aggatgccgt accgcgcgtg tacgatgctc ttgaggtgca aaatggtaat
    13801 gagcgtctgg tgctggaagt tcagcagcag ctcggcggcg gtatcgtgcg taccatcgca
    13861 atgggttcct ccgacggtct gcgtcgcggt ctggatgtaa aagacctcga acacccgatc
    13921 gaagtcccgg taggtaaagc gactctgggc cgtatcatga acgtactggg tgaaccggtc
    13981 gacatgaaag gcgagatcgg tgaagaagag cgttgggcga ttcaccgcgc agcaccttcc
    14041 tacgaagagc tgtcaaactc tcaggaactg ctggaaaccg gtatcaaagt tatcgacctg
    14101 atgtgtccgt tcgctaaggg cggtaaagtt ggtctgttcg gtggtgcggg tgtaggtaaa
    14161 accgtaaaca tgatggagct cattcgtaac atcgcgatcg agcactccgg ttactctgtg
    14221 tttgcgggcg taggtgaacg tactcgtgag ggtaacgact tctaccacga aatgaccgac
    14281 tccaacgtta tcgacaaagt atccctggtg tatggccaga tgaacgagcc gccgggaaac
    14341 cgtctgcgcg ttgctctgac cggtctgacc atggctgaga aattccgtga cgaaggtcgt
    14401 gacgttctgc tgttcgttga caacatctat cgttacaccc tggccggtac ggaagtatcc
    14461 gcactgctgg gccgtatgcc ttcagcggta ggttatcagc cgaccctggc ggaagagatg
    14521 ggcgttctgc aggaacgtat cacctccacc aaaactggtt ctatcacctc cgtacaggcg
    14581 gtatacgtac ctgcggatga cttgactgac ccgtctccgg caaccacctt tgcgcacctt
    14641 gacgcaaccg tggtactgag ccgtcagatc gcgtctctgg gtatctaccc ggccgttgac
    14701 ccgctggact ccaccagccg tcagctggac ccgctggtgg ttggtcagga acactacgac
    14761 accgcgcgtg gcgttcagtc catcctgcaa cgttatcagg aactgaaaga catcatcgcc
    14821 atcctgggta tggatgaact gtctgaagaa gacaaactgg tggtagcgcg tgctcgtaag
    14881 atccagcgct tcctgtccca gccgttcttc gtggcagaag tattcaccgg ttctccgggt
    14941 aaatacgtct ccctgaaaga caccatccgt ggctttaaag gcatcatgga aggcgaatac
    15001 gatcacctgc cggagcaggc gttctacatg gtcggttcca tcgaagaagc tgtggaaaaa
    15061 gccaaaaaac tttaacgcct taatcggagg gtgatatggc aatgacttac cacctggacg
    15121 tcgtcagcgc agagcaacaa atgttctctg gtctggtcga gaaaatccag gtaacgggta
    15181 gcgaaggtga actggggatc taccctggcc acgcaccgct gctcaccgcc attaagcctg
    15241 gtatgattcg catcgtgaaa cagcacggtc acgaagagtt tatctatctg tctggcggca
    15301 ttcttgaagt gcagcctggc aacgtgaccg ttctggccga caccgcaatt cgcggccagg
    15361 atctcgacga agcgcgagcc atggaagcga aacgtaaggc tgaagagcac attagcagct
    15421 ctcacggcga cgtagattac gctcaggcgt ctgcggaact ggccaaagcg atcgcgcagc
    15481 tgcgcgttat cgagttgacc aaaaaagcga tgtaacaccg gcttgaaaag cacaaaagcc
    15541 agtctggaaa caggctggct ttttttacgc gttgacccgt cctgaagagc gttcacatag
    15601 atcctgctga tataaaacac ccctgttttc ccgtttattc attgatcgaa ataagagcaa
    15661 aaacatccac ctgacgctta aatgaaggta ctgccttaat tttctgcaga caaaaggcgt
    15721 gacgatggtc gaaaatggca ctttcgtcag cggggataat ccgttattga acaatttatc
    15781 ctctgtccat ttcacgatga aaaaaatgta gttttttcaa ggtgaagcgg tttaaattcg
    15841 ttctcaaatt acagtcagga cgcgtatgtt gaataatgct atgagcgtag tgatccttgc
    15901 cgcaggcaaa ggcacgcgca tgtattccga tcttccgaaa gtgctgcata cccttgccgg
    15961 gaaagcgatg gttcagcatg tcattgatgc tgcgaatgaa ttaggcgcag cgcacgttca
    16021 cctggtgtac ggtcacggcg gcgatctgct taaacaggcg ctgaaagacg acaaccttaa
    16081 ctgggtgctt caggcagagc agctgggtac gggtcatgca atgcagcagg ccgcaccttt
    16141 ctttgccgat gatgaagaca ttttaatgct ctacggcgac gtgccgctga tctctgtcga
    16201 aacactccag cgcctgcgtg atgctaaacc ccagggtggc attggtctgc tgacggtgaa
    16261 actggatgat ccgaccggtt atggacgtat cacccgtgaa aatggcaaag ttaccggcat
    16321 tgttgagcac aaagatgcca ccgacgagca gcgtcagatt caggagatca acaccggcat
    16381 tctgattgcc aacggcgcag atatgaaacg ctggctggcg aagctgacca acaataatgc
    16441 tcagggcgaa tactacatca ccgacattat tgcgctggcg tatcaggaag ggcgtgaaat
    16501 cgtcgccgtt catccgcaac gtttaagcga agtagaaggt gtgaataacc gcctgcaact
    16561 ctcccgtctg gagcgcgttt accagtccga acaggctgaa aaactgctgt tagcaggcgt
    16621 tatgctgcgc gatccggcgc gttttgatct gcgcggtacg cttactcacg ggcgcgatgt
    16681 tgaaattgat actaacgtta tcatcgaggg caacgtgact ctcggtcatc gcgtgaaaat
    16741 cggcaccggt tgcgtgatta aaaacagcgt gattggcgat gattgcgaaa tcagtccgta
    16801 taccgttgtg gaagacgcga atctggcagc ggcctgtact attggcccgt ttgcccgtct
    16861 gcgtcctggt gctgagttgc tggaaggtgc acacgttggt aacttcgttg agatgaaaaa
    16921 agcgcgtctg ggtaaaggca cgaaagccgg gcatctgact tacctgggcg atgcggaaat
    16981 tggcgataac gttaacatcg gcgcgggaac cattacctgc aactacgatg gtgcgaataa
    17041 atttaagacc attatcggcg acgatgtgtt tgtcggttcc gacactcagc tggtggcccc
    17101 ggtaacagta ggcaaaggcg cgaccattgc tgcgggtaca actgtgacgc gtaatgtcgg
    17161 cgaaaatgca ttagctatca gccgtgtgcc gcagactcag aaagaaggct ggcgtcgtcc
    17221 ggtaaagaaa aagtaattct ggccgggtaa tccggtcatc tgggatgagg agataacata
    17281 atctccctcc cacaagcagt aactataaaa ataaccccac tctctacaag gctcggggcg
    17341 cccgaaaaaa cgggcataca ggttgaccga caacgatata aatcggaatc aaaaactatg
    17401 tgtggaattg ttggcgcgat cgcgcaacgt gatgtagcag aaatccttct tgaaggttta
    17461 cgtcgtctgg aataccgcgg atatgactct gccggtctgg ccgttgttga tgcggaaggt
    17521 catatgactc gcctgcgtcg cctcggtaaa gtccagatgc tggctcaggc agcggaagaa
    17581 catcctctgc atggcggcac cggtattgct cacactcgct gggcgaccca cggtgaacct
    17641 tcagaagcta acgcgcatcc gcatgtttct gaacacattg tggtggtgca taatggcatc
    17701 atcgaaaacc atgaaccgct gcgtgaagag ttaaaagcgc gtggctatac cttcgtttct
    17761 gaaaccgaca ccgaagtgat tgcccatctg gtgaactggg agctgaaaca aggcgggact
    17821 ctgcgtgagg ctgttctgcg tgctatcccg cagctgcgcg gtgcgtacgg tacagtgatc
    17881 atggactccc gtcacccgga taccctgctg gcggcacgtt ctggtagtcc gctggtgatt
    17941 ggcctgggta tgggcgaaaa ctttatcgct tctgaccagc tggcgctgtt gccggtgacc
    18001 cgtcgcttta tcttccttga agagggcgat attgcggaaa tcactcgccg ttcggtaaac
    18061 atcttcgata aaactggcgc ggaagtaaaa cgtcaggata tcgaatccaa tctgcaatat
    18121 gacgcgggcg ataaaggtat ttaccgtcac tacatgcaga aagagatcta cgaacagccg
    18181 aacgcgataa aaaacaccct taccgggcgt atcagccacg gtcaggttga tttaagcgag
    18241 ctgggaccga acgccgacga actgctgtcg aaggttgagc atattcagat cctcgcctgt
    18301 ggtacttctt ataactccgg tatggtttcc cgctactggt ttgaatcgct ggcaggtatt
    18361 ccgtgcgacg tcgaaatcgc ctctgaattc cgctatcgta aatctgccgt gcgtcgtaac
    18421 agcctgatga tcaccttgtc acagtctggc gaaaccgcgg ataccctggc gggcctgcgt
    18481 ctgtcgaaag agctgggtta ccttggttcg ctggcaatct gtaacgttcc gggttcttct
    18541 ctggtgcgcg aatccgatct ggcgctgatg accaacgcgg gtacggaaat cggcgtggcg
    18601 tccactaaag cattcaccac tcagttaact gtgctgttga tgctggtggc gaagctgtct
    18661 cgcctgaaag gtctggatgc ctccattgaa catgacattg tgcatggtct gcaggcgctg
    18721 ccgagccgta ttgagcagat gctgtctcag gataaacgca ttgaagctct ggcagaagat
    18781 ttctctgaca aacatcacgc gctgttcctg ggccgtggcg atcagtaccc aatcgcgctg
    18841 gaaggcgcat tgaagctgaa agagatctct tacattcacg ctgaagccta cgctgcaggc
    18901 gagctgaaac acggcccgct ggcgctgatt gacgcggata tgccggttat cgtcgttgca
    18961 ccgaacaacg aactgttgga aaaactgaaa tccaacattg aagaagttcg cgcgcgtggc
    19021 ggtcaattgt atgtcttcgc cgatcaggat gcgggttttg taagtagcga taacatgcac
    19081 atcatcgaga tgccgcatgt ggaagaggtg attgcaccga tcttctacac cgttccgctg
    19141 cagctgctgg cttaccatgt cgcgctgatc aaaggcaccg acgttgacca gccgcgtaac
    19201 ctggcaaaat cggttacggt tgagtaatca ctatcataga taaaaaaggg cttgtgggcg
    19261 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
    19321 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
    19381 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
    19441 gaggggcgtg gccaagggca tggtaaagac tatattccat ggctaacagt acaagaagtt
    19501 ccttcttcag gtcgttccca ccgtatttat tctcataaga cgggacgagt ccatcatttg
    19561 ctatctgact tagagcttgc tgtttttctc agtcttgagt gggagagcag cgtgctagat
    19621 atacgcgagc agttcccctt attacctagt gataccaggc agattgcaat agatagtggt
    19681 attaagcatc ctgttattcg tggtgtagat caggttatgt ctactgattt tttagtggac
    19741 tgcaaagatg gtccttttga gcagtttgct attcaagtca aacctgcagc agccttacaa
    19801 gacgagcgta ccttagaaaa actagaacta gagcgtcgct attggcagca aaagcaaatt
    19861 ccttggttca tttttactga taaagaaata aatcccgtag taaaagaaaa tattgaatgg
    19921 ctttattcag tgaaaacaga agaagtttct gcggagcttt tagcacaact atccccattg
    19981 gcccatatcc tgcaagaaaa aggagatgaa aacattatca atgtctgtaa gcaggttgat
    20041 attgcttatg atttggagtt aggcaaaaca ttgagtgaga tacgagcctt aaccgcaaat
    20101 ggttttatta agttcaatat ttataagtct ttcagggcaa ataagtgtgc agatctctgt
    20161 attagccaag tagtgaatat ggaggagttg cgctatgtgg caaattaatg aggttgtgct
    20221 atttgataat gatccgtatc gcattttggc tatagaggat ggccaagttg tctggatgca
    20281 aataagcgct gataaaggag ttccacaagc tagggctgag ttgttgctaa tgcagtattt
    20341 agatgaaggc cgcttagtta gaactgatga cccttatgta catcttgatt tagaagagcc
    20401 gtctgtagat tctgtcagct tccagaagcg cgaggaggat tatcgaaaaa ttcttcctat
    20461 tattaatagt aaggatcgtt tcgaccctaa agtcagaagc gaactcgttg agcatgtggt
    20521 ccaagaacat aaggttacta aggctacagt ttataagttg ttacgccgtt actggcagcg
    20581 tggtcaaacg cctaatgcat taattcctga ctacaaaaac agcggtgcac caggggaaag
    20641 acgttcagcg acaggaacag caaagattgg ccgagccaga gaatatggta agggtgaagg
    20701 aaccaaggta acgcccgaga ttgaacgcct ttttaggttg accatagaaa agcacctgtt
    20761 aaatcaaaaa ggtacaaaga ccaccgttgc ctatagacga tttgtggact tgtttgctca
    20821 gtattttcct cgcattcccc aagaggatta cccaacacta cgtcagtttc gttattttta
    20881 tgatcgagaa taccctaaag ctcagcgctt aaagtctaga gttaaagcag gggtatataa
    20941 aaaagacgta cgacccttaa gtagtacagc cacttctcag gcgttaggcc ctgggagtcg
    21001 ttatgagatt gatgccacga ttgctgatat ttatttagtg gatcatcatg atcgccaaaa
    21061 aatcatagga agaccaacgc tttacattgt gattgatgtg tttagtcgga tgatcacggg
    21121 cttttatatc ggctttgaaa atccgtctta tgtggtggcg atgcaggctt ttgtaaatgc
    21181 ttgctctgac aaaacggcca tttgtgccca gcatgatatt gagattagta gctcagactg
    21241 gccgtgtgta ggtttgccag atgtgttgct agcggaccgt ggcgaattaa tgagtcatca
    21301 ggtcgaagcc ttagtttcta gttttaatgt gcgagtggaa agtgctccac ctagacgtgg
    21361 cgatgctaaa ggcatagtgg aaagcacttt tagaacacta caagccgagt ttaagtcctt
    21421 tgcacctggc attgtagagg gcagtcggat caaaagccat ggtgaaacag actataggtt
    21481 agatgcatct ctgtcggtat ttgagttcac acaaattatt ttgcgtacga tcttattcag
    21541 aaataaccat ctggtgatgg ataaatacga tcgagatgct gattttccta cagatttacc
    21601 gtctattcct gtccagctat ggcaatgggg tatgcagcat cgtacaggta gtttaagggc
    21661 tgtggagcaa gagcagttgc gagtagcgtt actgcctcgc cgaaaggtct ctatttcttc
    21721 atttggcgtt aatttgtggg gtttgtatta ctcggggtca gagattctgc gtgagggttg
    21781 gttgcagcgg agcactgata tagctagacc tcaacattta gaagcggctt atgacccagt
    21841 gctggttgat acgatttatt tgtttccgca agttggcagc cgtgtatttt ggcgctgtaa
    21901 tctgacggaa cgtagtcggc agtttaaagg tctctcattt tgggaggttt gggatataca
    21961 agcacaagaa aaacacaata aagccaatgc gaagcaggat gagttaacta aacgcaggga
    22021 gcttgaggcg tttattcagc aaaccattca gaaagcgaat aagttaacgc ccagtactac
    22081 tgagcccaaa tcaacacgca ttaagcagat taaaactaat aaaaaagaag ccgtgacctc
    22141 ggagcgtaaa aaacgtgcgg agcatttgaa gccaagctct tcaggtgatg aggctaaagt
    22201 tattcctttc aacgcagtgg aagcggatga tcaagaagat tacagcctac ccacatacgt
    22261 gcctgaatta tttcaggatc caccagaaaa ggatgagtca tgagtgctac ccggattcaa
    22321 gcagtttatc gtgatacggg ggtagaggct tatcgtgata atccttttat cgaggcctta
    22381 ccaccattac aagagtcagt gaatagtgct gcatcactga aatcctcttt acagcttact
    22441 tcctctgact tgcaaaagtc ccgtgttatc agagctcata ccatttgtcg tattccagat
    22501 gactattttc agccattagg tacgcatttg ctactaagtg agcgtatttc ggtcatgatt
    22561 cgaggtggct acgtaggcag aaatcctaaa acaggagatt tacaaaagca tttacaaaat
    22621 ggttatgagc gtgttcaaac gggagagttg gagacatttc gctttgagga ggcacgatct
    22681 acggcacaaa gcttattgtt aattggttgt tctggtagtg ggaagacgac ctctcttcat
    22741 cgtattctag ccacgtatcc tcaggtgatt taccatcgtg aactcaatgt agagcaggtg
    22801 gtgtatttga aaatagactg ctcgcataat ggttcgctaa aagaaatctg cttgaatttt
    22861 ttcagagcgt tggatcgagc cttgggctcg aactatgagc gtcgttatgg cttaaaacgt
    22921 catggtatag aaaccatgtt ggctttgatg tcgcaaatag ccaatgcaca tgctttaggg
    22981 ttgttggtta ttgatgaaat tcagcattta agccgctctc gttcgggtgg atctcaagag
    23041 atgctgaact tttttgtgac gatggtgaat attattggcg taccagtgat gttgattggt
    23101 acccctaaag cacgagagat ttttgaggct gatttgcggt ctgcacgtag aggggcaggg
    23161 tttggagcta tattctggga tcctatacaa caaacgcaac gtggaaagcc caatcaagag
    23221 tggatcgctt ttacggataa tctttggcaa ttacagcttt tacaacgcaa agatgcgctg
    23281 ttatcggatg aggtccgtga tgtgtggtat gagctaagcc aaggagtgat ggacattgta
    23341 gtaaaacttt ttgtactcgc tcagctccgt gcgctagctt taggcaatga gcgtattacc
    23401 gctggtttat tgcggcaagt gtatcaagat gagttaaagc ctgtgcaccc catgctagag
    23461 gcattacgct cgggtatccc agaacgcatt gctcgttatt ctgatctagt cgttcccgag
    23521 attgataaac ggttaatcca acttcagcta gatatcgcag cgatacaaga acaaacacca
    23581 gaagaaaaag cccttcaaga gttagatacc gaagatcagc gtcatttata tctgatgctg
    23641 aaagaggatt acgattcaag cctgttaatt cccactatta aaaaagcgtt tagccagaat
    23701 ccaacgatga caagacaaaa gttactgcct cttgttttgc agtggttgat ggaaggcgaa
    23761 acggtagtgt cagaactaga aaagccctcc aagagtaaaa aggtttcggc tataaaggta
    23821 gtcaagccca gcgactggga tagcttgcct gatacggatt tacgttatat ctattcacaa
    23881 cgccaacctg aaaaaaccat gcatgaacgg ttaaaaggga aaggggtaat agtggatatg
    23941 gcgagcttat ttaaacaagc aggttagcca tgagaaactt tcctgttccg tactcgaatg
    24001 agctgattta tagcactatt gcacgggcag gcgtttatca agggattgtt agtcctaagc
    24061 agctgttgga tgaggtgtat ggcaaccgca aggtggtcgc taccttaggt ctgccctcgc
    24121 atttaggtgt gatagcaaga catctacatc aaacaggacg ttacgctgtt cagcagctta
    24181 tttatgagca taccttattc cctttatatg ctccgtttgt aggcaaggag cgccgagacg
    24241 aagctattcg gttaatggag taccaagcgc aaggtgcggt gcatttaatg ctaggagtcg
    24301 ctgcttctag agttaagagc gataaccgct ttagatactg ccctgattgc gttgctcttc
    24361 agctaaatag gtatggggaa gccttttggc aacgagattg gtatttgccc gctttgccat
    24421 attgtccaaa acacggtgct ttagtcttct ttgatagagc tgtagatgat caccgacatc
    24481 aattttgggc tttgggtcat actgagctgc tttcagacta ccccaaagac tccctatctc
    24541 aattaacagc actagctgct tatatagccc ctctgttaga tgctccacga gcgcaagagc
    24601 tttccccaag ccttgagcag tggacgctgt tttatcagcg cttagcgcag gatctagggc
    24661 taaccaaaag caagcacatt cgtcatgact tggtggcgga gagagtgagg cagactttta
    24721 gtgatgaggc actagagaaa ctggatttaa agttggcaga gaacaaggac acgtgttggc
    24781 tgaaaagtat attccgtaag catagaaaag cctttagtta tttacagcat agtattgtgt
    24841 ggcaagcctt attgccaaaa ctaacggtta tagaagcgct acagcaggca agtgctctta
    24901 ctgagcactc tataacgaca agacctgtta gccagtctgt gcaacctaac tctgaagatt
    24961 tatctgttaa gcataaagac tggcagcaac tagtgcataa ataccaagga attaaggcgg
    25021 caagacagtc tttagagggt ggggtgctat acgcttggct ttaccgacat gacagggatt
    25081 ggctagttca ctggaatcaa cagcatcaac aagagcgtct ggcacccgcc cctagagttg
    25141 attggaacca aagagatcga attgctgtac gacaactatt aagaatcata aagcgtctag
    25201 atagtagcct tgatcaccca agagcgacat cgagctggct gttaaagcaa actcctaacg
    25261 gaacctctct tgcaaaaaat ctacagaaac tgcctttggt agcgctttgc ttaaagcgtt
    25321 actcagagag tgtggaagat tatcaaatta gacggattag ccaagctttt attaagctta
    25381 aacaggaaga tgttgagctt aggcgctggc gattattaag aagtgcaacg ttatctaaag
    25441 agcggataac tgaggaagca caaagattct tggaaatggt ttatggggaa gagtgagtgg
    25501 ttaggctagc tacatttaat gacaatgtgc aggttgtaca tattggtcat ttattccgta
    25561 actcgggtca taaggagtgg cgtatttttg tttggtttaa tccaatgcaa gaacggaaat
    25621 ggactcgatt tactcatttg cctttattaa gtcgagctaa ggtggttaac agtacaacaa
    25681 agcaaataaa taaggcggat cgtgtgattg agtttgaagc atcggatctt caacgagcca
    25741 aaataatcga ttttcctaat ctctcgtcct ttgcttccgt acgcaacaag gatggagcgc
    25801 agagttcatt tatttacgaa gctgaaacac catatagcaa gactcgttat cacatcccac
    25861 agttagagct agctcggtca ttatttttaa ttaactccta tttctgtcga agctgtttga
    25921 gcagtaccgc tttacagcaa gagttcgacg ttcagtatga ggttgagcga gatcatttag
    25981 agataaggat cttacccagt tcatcgtttc ctaaaggggc gttagagcag tcggccgtag
    26041 tgcagctttt ggtttggttg ttttcggatc aagatgttat ggattcgtat gaaagtattt
    26101 ttaggcacta tcaacaaaat agagaaatta agaacggcgt tgaaagctgg tgctttagct
    26161 ttgaccctcc gcccatgcag ggttggaaat tacatgtaaa aggacgttct tctaacgagg
    26221 ataaggatta tttagttgag gaaatagtag gtttagaaat caacgctatg cttcctagca
    26281 caacagctat tagccatgcc tcttttcagg aaaaggaggc aggtgatggt agtacgcagc
    26341 acatagcggt ttcaacagag tcagttgttg atgatgagca tctacagttg gacgatgagg
    26401 aaacagccaa tatagacaca gacacacgag tcatagaggc tgagccgaca tggataagtt
    26461 ttagtagacc tagtcgaatt gaaaaatctc gcagggcaag aaaaagtagc caaactattt
    26521 tagaaaaaga agaagcaaca acaagtgaaa atagtaattt ggttagtact gatgagccac
    26581 acttaggtgg tgtcctagca gcggcagatg tgggtgggaa gcaggatgca accaattaca
    26641 actctatttt tgctaatcga tttgctgctt ttgatgagct actttcaatt ctaaaaacta
    26701 aatttgcatg tcgggtgctt tttgaagaaa ccttggtttt gccaaaagtt gggcgtagcc
    26761 gattacatct gtgtaaagat ggctcaccaa gagtgattaa agccgttggg gtgcaacgta
    26821 atggcagtga atttgtattg ctagaggtgg atgcatcgga tggggtgaaa atgctttcta
    26881 ccaaagtgtt gagtggcgtt gatagcgaaa catggcggaa tgattttgaa aagatacggc
    26941 gtggagtggt gaagagctca ttgaattggc caaatagttt gtttgatcaa ttatatggac
    27001 aagacgggca tagaggggtg aatcatccaa aggggttggg ggagctgcaa gtatcgagag
    27061 aggatatgga agggtgggct gagagagtgg ttagagagca atttacgcat taaaggaatg
    27121 actgaaagag cctgtaaacc cttttgtgta agtgcttttg ccggtcagtt aaaggtggcc
    27181 atttaaacgg tcaccaaatt cgatcataaa acggttcatg gccggcttcc agttgcggat
    27241 cggcatcgtc catttcttgg tcgccgcctg gatagccagg tacaccacct tcatcgctga
    27301 ttcgtccgta gggaacacct tgagttcata ttaatggaat tttctacaaa tagcctccgt
    27361 ggttttgagg ggggattaca gacgatccat agtagtaatc caatgagttc ttgagcgcgg
    27421 cgacatgttt ggacgccttg gcaaaaatta gagcctgctt gaagtgcagg cgagcacgtg
    27481 cttggacgat tgatccattc gcggtcaaaa actcaatctt ggatgacagc gtgtcaggaa
    27541 atccaatatc gaagtcccgc cccgtgtcaa agtagggcat ttcatgatca aaggacgaag
    27601 cttcccaggc cacttcccta atattccagc cgctagaaac aagatttcgt acgacttcat
    27661 caactttttt gctttccaac ttgtcacaaa gtgcataggc aagagctttg gcggcagaca
    27721 cgtgcgtttc ctcttaaaca taggtcgaac ggatacccga ggcaaagcgg agcattagtc
    27781 ccaaagcatc tccggtagat ccggaggctc catactgaag gcatggcatc ttgggcattg
    27841 atatttgaaa cttctctcta gatagcaggg atttcttggc cttgcctcat gctgatagct
    27901 tgtaaatatc acacgatcct tagccccaca aagtgagcat actccgtagc tttcatttat
    27961 agaaatcgtc gttagattgt cgcagcatgt gcacgttccg actatttgct caggaagcat
    28021 cgccatacct tggcctaggt aaagaagatc agactcgtag ccacatcggt ggcaactgag
    28081 cttgtacgca atgcccatct ggttgtctca cctgaatcgt aacgtcagag ctattcatcc
    28141 atccattcgg catcaatgta tgccactaag ccgaccagct gaccgtttgg actgaagtat
    28201 gttgcctgca ccccacttga catctcatca ataccaccgc actgcgctag gcgccggtcc
    28261 cacttgccca cagaagcgtc aggatcggct gagttcccca agacccttgt gagccatgcc
    28321 tcctttaact caacctctcg aatgattgcg tcggccagct cctccgcgtt atctagttcg
    28381 acatagtctg gaattcggac gtacgttctg aacacagctg gatacttact tgggcgattg
    28441 tcatacatga catcaacaat gtacccgttt gtgtaaccgt ctcttggagg ttcgtatgac
    28501 actagtggtt cccctcagct tgcgactaga tgttgaggcc taacatttta ttagagagca
    28561 ggctagttgc ttagatacat gatcttcagg ccgttatctg tcagggcaag cgaaaattgg
    28621 ccatttatga cgaccaatgc cccgcagaag ctcccatctt tgccgccata gacgccgcgc
    28681 cccccttttg gggtgtagaa catccttttg ccagatgtgg aaaagaagtt cgttgtccca
    28741 ttgttggcaa tgacgtagta gccggcgaaa gtgcgagacc catttgcgct atatataagc
    28801 ctacgatttc cgttgcgact attgtcgtaa ttggatgaac tattatcgta gttgctctca
    28861 gagttgtcgt aatttgatgg actattgtcg taattgctta tggagttgtc gtagttgctt
    28921 ggagaaatgt cgtagttgga tggggagtag tcatagggaa gacgagcttc atccactaaa
    28981 acaattggca ggtcagcaag tgcctgcccc gatgccatcg caagtacgag gcttagaacc
    29041 accttcaaca gatcgcgcat agtcttcccc agctctctaa cgcttgagtt aagccgcgcc
    29101 gcgaagcggc gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc
    29161 tcgcctttca cgtagtgaac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
    29221 tcttgtccaa gataagcctg cctagcttca agtatgacgg gctgatactg ggccggcagg
    29281 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
    29341 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
    29401 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
    29461 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
    29521 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
    29581 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
    29641 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
    29701 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
    29761 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
    29821 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
    29881 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt taactcctga
    29941 attaagccgc gccgcgaagc ggtgtcggct tgaatgaatt gttaggcgtc atcctgtgct
    30001 cccgagaacc agtaccagta catcgctgtt tcgttcgaga cttgaggtct agttttatac
    30061 gtgaacaggt caatgccgcc gagagtaaag ccacattttg cgtacaaatt gcaggcaggt
    30121 acattgttcg tttgtgtctc taatcgtatg ccaaggagct gtctgcttag tgcccacttt
    30181 ttcgcaaatt cgatgagact gtgcgcgact cctttgcctc ggtgcgtgtg cgacacaaca
    30241 atgtgttcga tagaggctag atcgttccat gttgagttga gttcaatctt cccgacaagc
    30301 tcttggtcga tgaatgcgcc atagcaagca gagtcttcat cagagtcatc atccgagatg
    30361 taatccttcc ggtaggggct cacacttctg gtagatagtt caaagccttg gtcggatagg
    30421 tgcacatcga acacttcacg aacaatgaaa tggttctcag catccaatgt ttccgccacc
    30481 tgctcaggga tcaccgaaat cttcatatga cgcctaacgc ctggcacagc ggatcgcaaa
    30541 cctggcgcgg cttttggtac aaaaggcgtg acaggtttgc gaatccgttg ctgccacttg
    30601 ttaacccttt tgccagattt ggtaactata atttatgtta gaggcgaagt cttgggtaaa
    30661 aactggccta aaattgctgg ggatttcagg aaagtaaaca tcaccttccg gctcgatgtc
    30721 tattgtagat atatgtagtg tatctacttg atcgatcagg cttttgtata tctccccacc
    30781 acctgaaaca atgacatgat ccgttatttt ctttaggttg gttaaagcat ctttaattga
    30841 tggaaagatc aatacgttct cattgtcaga tgtaaaactt gaacgtgtta cgaccgcata
    30901 ctttcggttg ggtaatgctc ccattgattc aaaagtcttg cgtccaacca acagccattg
    30961 gttataggta atagctttaa acaggagctg ttcacctttg gcactccatg gaatatcagg
    31021 gccattcccg ataactccat tcttcgatat agctaccatt agtgatagtt tcacaattct
    31081 tcctcagagg ttaacatttt attagtaagc atgctcgttt tgtcacccgc tgatgcttac
    31141 cgttaattaa tataatcact tgtattaaat agactttctt taaaaataca agacactctg
    31201 ttattacaaa tcgtgcatgc cgtctatcct gcttgcacga tgcacattat ctcacccaaa
    31261 gaactcataa cattttaatt tatttagcat taaattaaaa ctctaccggt aaacaagcat
    31321 ctctaggcgt aaaaaacagg cactttttta aatcccagct agaataggct gtataggcag
    31381 acagttgcaa gacaaggagc gttttatgtc taacagtcca tttttaaatt ctatacgcac
    31441 ggatatgcga caaaaaggtt atgcgctgaa aactgaaaaa acttacctgc actggattaa
    31501 gcgttttatt ctgtttcaca aaaaacgtca tcctcagacc atgggcagtg aagaggtcag
    31561 gctgttttta tccagcttag caaacagcag acatgtagcc ataaacacgc agaaaatcgc
    31621 tttaaatgcc ctagcttttt tgtacaacag gtttttacaa cagccgttgg gcgatattga
    31681 ttatatccct gcaagcaagc ctagacggct accctctgtt atctctgcaa atgaagtgca
    31741 acgcattttg caggttatgg atactcgcaa ccaagttatt tttacgctgc tgtatggtgc
    31801 aggtttgcgc attaatgaat gcttgcgttt gcgggttaaa gattttgatt ttgataatgg
    31861 ctgcatcact gtgcatgacg gtaagggtgg gaaaagcaga aacagcctac tgcccacgcg
    31921 cctaatccca gcaataaaat aactcattga gcaagcgcgg cttattcagc aagacgacaa
    31981 cttacaaggc gtagggccat cgctgccttt tgctttagat cacaaatacc cttctgctta
    32041 tcgacaagcg gcgtggatgt ttgtctttcc ctccagcacg ctctgcaacc acccgtataa
    32101 cggcaaatta tgccgccatc atctgcatga ctccgttgcg cgaaaggcat tgaaggcagc
    32161 cgtacaaaaa gcaggcatcg ttagcaagcg tgtcacttgt catacatttc gtcactcgtt
    32221 tgctacgcat ctattacaag cggggcgtga tattcgcact gtgcaagaac tcttagggca
    32281 taacgatgtt aagaccacgc aaatctatac gcatgtgttg ggtcagcatt ttgccggcac
    32341 caccagtcct gcggatggac tgatgctact tatcaatcag taaaagtaga taaatatctt
    32401 tatgtagccc tgagtagccc ctgatacttt ttatggtgaa aaagataaac aatcatagag
    32461 gggataaata tgagccagtt ctaccattgt tcagataagt ttaagcaaga tactgtcaat
    32521 cacagtgacc attcatggtc attacacttc ctccagttgc cagacaatgc ctaacataaa
    32581 aacgcaaaag cccaaaagtc agagcttagg tgttctaggc gttttctaag cgctgaagaa
    32641 atacgtctta ccgtagattg cgtgcgtttc tgaagcacat tttccttata catggcttta
    32701 tcccaggcag cttcatcaag ctcttggagt aacaagccag taataatgcg acactctcgg
    32761 gtttgtagcg agccgctggt gagatcggag ttatagcgga aaggggtcat ttgtaattat
    32821 ttgtgggtgg aggatagtta acttattatt gtagttggaa gaggctgctt tccaaacatt
    32881 agcacctcgt aatgcttgtt actgtatatt aatttatgct cttagcgcat gttaatgggc
    32941 tattacaatg cataatgtcc acatcaagat aactgtggct taacacctgt acaagcgaaa
    33001 aaaatcaatc acctgtccgg aaatacggga tcactgtaaa gtgttggtga ggcagtctag
    33061 gagggggatt taatttggga tactgatgaa aggattatga tcaattggat ataggatcgt
    33121 actaagctga gcggcaggtt agctagatta gttaaccaga taagtgaaat ctagttccaa
    33181 actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta
    33241 ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca
    33301 actattttgt ccgcccac
//
LOCUS       DAJVPP010000040        32919 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVPP010000040 DAJVPP010000000
VERSION     DAJVPP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.040"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(2..94)
                     /locus_tag="OQM34_001835"
     tRNA            complement(2..94)
                     /locus_tag="OQM34_001835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OQM34_001836"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OQM34_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1452314.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OQM34_001837"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OQM34_001837"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1452315.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OQM34_001838"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OQM34_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1452316.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OQM34_001839"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OQM34_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1452317.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OQM34_001840"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OQM34_001840"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1452318.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OQM34_001841"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OQM34_001841"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1452319.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OQM34_001842"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OQM34_001842"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OQM34_001843"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OQM34_001843"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1452320.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OQM34_001844"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OQM34_001844"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1452321.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OQM34_001845"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OQM34_001845"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCS1452322.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OQM34_001846"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OQM34_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OQM34_001847"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OQM34_001847"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1452323.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMAPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OQM34_001848"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OQM34_001848"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1452324.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OQM34_001849"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OQM34_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1452325.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OQM34_001850"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OQM34_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1452326.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OQM34_001851"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OQM34_001851"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCS1452327.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OQM34_001852"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OQM34_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1452328.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OQM34_001853"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OQM34_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS1452329.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OQM34_001854"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OQM34_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1452330.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OQM34_001855"
     CDS             complement(20900..21019)
                     /locus_tag="OQM34_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCS1452331.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OQM34_001856"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OQM34_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1452332.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OQM34_001857"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OQM34_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1452333.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25505)
                     /gene="hycC"
                     /locus_tag="OQM34_001858"
     CDS             complement(23682..25505)
                     /gene="hycC"
                     /locus_tag="OQM34_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS1452334.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLF
                     KSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWV
                     IYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENA
                     TCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIA
                     CPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLR
                     KWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25542..26153)
                     /gene="hycB"
                     /locus_tag="OQM34_001859"
     CDS             complement(25542..26153)
                     /gene="hycB"
                     /locus_tag="OQM34_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS1452335.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26278..26739)
                     /gene="hycA"
                     /locus_tag="OQM34_001860"
     CDS             complement(26278..26739)
                     /gene="hycA"
                     /locus_tag="OQM34_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS1452336.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26951..27301
                     /gene="hypA"
                     /locus_tag="OQM34_001861"
     CDS             26951..27301
                     /gene="hypA"
                     /locus_tag="OQM34_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1452337.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27305..28177
                     /gene="hypB"
                     /locus_tag="OQM34_001862"
     CDS             27305..28177
                     /gene="hypB"
                     /locus_tag="OQM34_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1452338.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28168..28440
                     /gene="hypC"
                     /locus_tag="OQM34_001863"
     CDS             28168..28440
                     /gene="hypC"
                     /locus_tag="OQM34_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1452339.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28440..29561
                     /gene="hypD"
                     /locus_tag="OQM34_001864"
     CDS             28440..29561
                     /gene="hypD"
                     /locus_tag="OQM34_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1452340.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29558..30568
                     /gene="hypE"
                     /locus_tag="OQM34_001865"
     CDS             29558..30568
                     /gene="hypE"
                     /locus_tag="OQM34_001865"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1452341.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30642..32720
                     /gene="flhA"
                     /locus_tag="OQM34_001866"
     CDS             30642..32720
                     /gene="flhA"
                     /locus_tag="OQM34_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCS1452342.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32757..>32919)
                     /locus_tag="OQM34_001867"
     CDS             complement(32757..>32919)
                     /locus_tag="OQM34_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1452343.1"
                     /translation="YQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRPVPELPE
                     KYRPKKPRE"
BASE COUNT         7755 a         9182 c         8583 g         7399 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgacatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggctc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgagcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttataccca gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagccccag caggataatg
    24661 ccgatatttt ccagggtgtg gtaagccagc aggcgctgga tgttgtgttc catcaacgca
    24721 tacagaccac cgacgaacgc ggtgatcatg ccgagcacca gcagcgcgat cccccaccac
    24781 agcggtgcat taccgcccag cagtgacagg gttaacatgc ccagcaggcc aattttcatg
    24841 actaccgtag aaaacaacgc ggcagctggc gcagaggcgt tagcatgtgc ctgcggcacc
    24901 cagccgtgca gcggaataat cccagccagc aggccaaagc caatcacacc gagcagccag
    24961 atatcggaac cgagcggcag ctgttgcata cgcatatcca gcaggcgcag atccagcgtg
    25021 ccataacgct gccacagcag ccagcaagca atcaccagca gcagagtgcc aagacgcccc
    25081 agcgcaaacc acagtttgcc ctctttgctg ttgctggtga ggaacaccgc acacaaggcc
    25141 atgacttcgg ccattaccac gaacatgccg aggttgctgg cgatgacggc gcagacggcg
    25201 gcagccatca acatattgat ctgcaagccg ttgcacttca cctgcgcgtg gcgatgccag
    25261 tcaatgttat agaggctgac aaacagaccg caaaggccga gggtaatcag ccaaatcgcg
    25321 ttaagcgggg agatttgcac atcgtagctt accagcgaca gcgcaccgct cacgccaacc
    25381 gcgccagtca gtacagtgaa gcccgcggct gccgtataca gactaccaac cgcgccgcca
    25441 attccggcta tccagccact tagcgctttt tgaaaagaga aatgccagaa cagcggcggc
    25501 gacaaaccat gccacgccgc tattgatcag ggaaattgcg ctcatttagc ctctccactt
    25561 tgagcctgct gaaacaaggt gagatcgcca aagtccgtgt taaaggtcag ctcacgctta
    25621 cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt agtcgggcac
    25681 atccgcacac aggccggacc ttgttcatca aagctacaaa ggtcacattt gacggcgatc
    25741 gcgcgaatac ctggcaccca gtcaagcaat gtgctgacac gcgccggagc aggcggtgcc
    25801 ggtggcgctt tcggggtatt ggcgtttgcc ggaatatcca gcggacggct gccggaaaat
    25861 tcaattgcgc caaacgggca ggcgatgccg cacagtttgc agcttacgca cagactttca
    25921 ttcaactgca cggccccatc aacgcgggtg atggcgttaa ccgggcagac caccgcgcag
    25981 ggtgcatctt cacagtggtg acagagctgc ggcgcagatt ctttttcatt gagcatcact
    26041 ctcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca ggcggcctca
    26101 caggtgtggc agccgataca gagcgtggag tcggcaatta caaaacgatt caccaggcat
    26161 tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt ttcgacactc
    26221 atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag cctcagctca
    26281 tgctgccggg ctttgtccct ttaccagttg gcttaaattc accggcacat tgttttcaat
    26341 ggcggtgtat aagctgtcgt aaacgccagc gattttttcg agatagtgtt ccagaacctg
    26401 ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca gttgcgcctg
    26461 cgcaatccgc tgtttgtctt cgccatcttt tgtttcgacg taatactcga tggtgtggct
    26521 gttaaagcca tccgccaggg tgacgtaaat acgaaaatgt tcaaaaagga cgaaacagag
    26581 ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata acgtgatccc
    26641 ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt aggcgacgat gccgctgtgc
    26701 gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac tcgtttaaca
    26761 gagatgccag ctttaagcat tttttgtgcc aacttttaat ttattgttat taaagagatt
    26821 tttaagctaa agatgaattt cgtcgccgtg tcgacgtgtc atttcgacat catcgacatt
    26881 attcaccgca gggataatca acactggcac aattattgct tgtagctggc aatagttaat
    26941 gggaggcgat atgcacgaaa taaccctctg ccaacgggca ctggaattga tcgaacagca
    27001 ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg gcgcattttc
    27061 ttgtgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg gcagcgtggc
    27121 ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa tgctggtgtg aaacatgcca
    27181 acagtatgtg acgctactga cccagcgcgt ccgccgctgt ccacagtgtc atggtgacat
    27241 gctacagatt gtggcagacg acggtttaca gattcggcgg atagaaatag accaggagtg
    27301 agcgatgtgt acaacatgcg gttgcggtga aggcaacctg tatatcgagg gtgatgaaca
    27361 taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc cgcagatgaa
    27421 aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag gcgacctgca
    27481 ttacggtcat ggcgaagcgg gcactcacac accgggcatg agccagcgtc ggatgctgga
    27541 agtcgaaatt gacgtgctgg acaaaaacaa ccgtctggct gaacgcaacc gcgcgcgctt
    27601 tgctgcccgc aagcaactgg tgctcaacct ggtttccagc cctggttccg gtaaaaccac
    27661 cctgctgacg gaaaccttaa tgcgcctgaa agacagcgtt ccgtgcgcgg ttattgaagg
    27721 cgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggtacac cagcgattca
    27781 ggtgaacacc ggtaaaggct gccatcttga cgcacagatg attgccgacg ccgcaccgcg
    27841 tctgccactg gacgataacg gtattctgtt tatcgaaaac gttggcaacc tcgtatgccc
    27901 ggccagcttc gatctcggtg aaaaacacaa agtagcggtg ctttccgtta ccgaaggtga
    27961 agacaaaccg ctgaaatatc cgcatatgtt tgccgccgcc tcgctgatgc tgctcaacaa
    28021 agttgacctg ttgccgtatc tcaactttga cgttgagaag tgcatcgcct gcgcccgcga
    28081 agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggcgaag ggatggacca
    28141 gtggctgaac tggctggaga cacagcgatg tgcataggcg ttcccggcca gatccgcacc
    28201 attgacggca accaggcgaa agtcgacgtc tgcggcattc agcgcgatgt cgatttaacg
    28261 ttagtcggca gctgcgatga aaacggtcag ccgcgcgtgg gccagtgggt actggttcac
    28321 gttggctttg ccatgagcgt aattaatgaa gccgaagcac gcgacactct cgacgcctta
    28381 caaaacatgt ttgacgttga gccggatgtc ggcgcgctgt tgtatggcga ggaaaaataa
    28441 tgcgttttgt tgatgaatat cgcgcgccgg aacaggtgat gcagttaatt gagcatctgc
    28501 gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt atggaagtgt
    28561 gtggcggtca tacccacgcc atttttaaat tcggcctcga ccagttactg cctgaaaacg
    28621 ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact gccgatgggt agaatcgaca
    28681 cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc gacgccatgc
    28741 gcgtaccagg gaaacaggga tcgctgttgc aggcaaaagc acgcggtgcc gatgtgcgca
    28801 tcgtttactc gccgatggat gcgttgaaac tggcgcagga gaatccaacc cgcaaagtgg
    28861 tgttcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact ctgcaacagg
    28921 cgaaagcgcg tgatgtgcag aatttttact tcttctgcca gcacattacg cttatcccga
    28981 cgctgcgcag tttgctggaa cagccggata acggtatcga cgcgttcctc gcgccgggcc
    29041 acgttagtat ggttatcggc actgatgcct ataattttat cgccagcgat tttcatcgtc
    29101 cgctggtggt cgctggtttc gaaccccttg atctactgca aggcgtggtc atgctggtgg
    29161 agcagaaaat agcggcccac agcaaggtag agaatcagta tcgtcgggtg gtaccggatg
    29221 ccggtaacct gctggcgcaa caggcgattg ccgatgtgtt ctgtgtcaac ggcgacagcg
    29281 aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg gattatcaac
    29341 gattcgatgc cgaagcacat ttccgcccgg caccgcagca ggtctgcgat gacccgcgcg
    29401 cgcgttgtgg tgaggtatta acgggcaaat gtaagccgca tcaatgcccg ctgtttggta
    29461 acacctgtaa tcctcaaacc gcatttggtg cactgatggt ctcctccgaa ggagcgtgcg
    29521 ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc aactcgccca
    29581 cggcagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg aagcctttgc
    29641 caacccgtgg ctggcagagc aggaagatca ggcacgtctt gatctggcgc agctggtagc
    29701 ggaaggcgac cgtctggcgt tctccaccga cagttacgtc attgacccgc tgttctttcc
    29761 tggcggtaat atcggcaagc tggcgatttg cggcaccgcg aatgacgttg cgggcagtgg
    29821 cgctattccg cgctatctct cctgtggctt tatcctcgaa gaaggattgc cgatggagac
    29881 actgaaagcc gtagtgacca gcatggcaga aaccgcccgc acggcaggca ttgccatcgt
    29941 tactggcgat actaaagtgg tgcagcgcgg cgcggcagat aaactgttta tcaacaccgc
    30001 tggcatgggc gcaattccgg cgaatattca ctggggcgca cagacgctaa ccgcaggcga
    30061 tgtattgctg gttagcggta cactcggcga ccacggggcg actatcctta acctgcgtga
    30121 gcagctgggg ctggatggcg aactggtcag cgactgcgcg gtgctgacgc cgcttattca
    30181 gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg gtggtgtaaa
    30241 cgcggtggtt catgagttcg cggcagcctg cggttgtggt attgaaattt ctgaatcagc
    30301 gctgccggtt aaacctgccg tgcgcggcgt ttgcgaattg ctgggactgg acgccctgaa
    30361 ctttgccaac gaaggcaaac tggtgatcgc cgttgaacgc aacgcggcag agcaagtgct
    30421 ggcagcgtta cattcccatc cactggggaa agacgcggcg ctgattggtg aagtggtgga
    30481 acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa cgaaccctcg atttaccaca
    30541 cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag ttagtcaatg
    30601 accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat acaccgatga
    30661 gtgatctcgg acagcaaggg ttgttcgaca tcactcggac actattgcag cagcccgatc
    30721 tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc gccgacaacg
    30781 cggctattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgcg tcgcgtgaaa
    30841 aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg gtacgcagca
    30901 ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga attttgtgaa acctggccgc
    30961 agctggccgc aggtgggcta taccccaaat ttggtcacta ttgcctgatg ccactggcgg
    31021 cgggagggca tatttttggt ggctgtgaat ttattcgtta tgacgagcgt ccctggagcg
    31081 aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc accgaacaaa
    31141 tccagagccg cgtcgtcaac aatgtcgact atgagttgtt atgccgggag cgcgataact
    31201 tccgcatcct ggttgccatt accaacgcgg tgctttcccg cctggatatg gacgaactgg
    31261 tcagcgaagt cgccaaagaa atccattact atttcgacat tgacgatatc agcattgtct
    31321 tacgcagcca ccgtaaaaac aaactcaaca tctactccac tcactatctt gataaacaac
    31381 atcccgccca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa cgcgtgttca
    31441 aaagtaaaga gatgctgctg atcaatctct acgagcggga cgatttagcc ccctatgaac
    31501 gcatgttgtt cgacacctgg ggcaaccaga ttcaaacctt gtgcctgtta ccgctgatgt
    31561 ctggcgacac catgctgggc gtgctgaaac tggcgcaatg cgaagagaaa gtgtttacca
    31621 ctaccaatct gaatttgctg cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg
    31681 ccctcgccta tcaggaaatc catcgtctga aagaacggct ggttgatgaa aacctcgccc
    31741 tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc cgcagcgaag
    31801 ccatgtacag cgtgcttaaa caagttgaaa tggtggcgca aagtgacagt accgtgctga
    31861 tcctcggtga aactggctcg ggtaaagagc tgattgcccg tgcgatccat aatctcagtg
    31921 ggcgtaataa tcgccgcatg gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg
    31981 aaagcgatct gtttggtcat gagcgtgggg cttttaccgg tgccagcgcc cagcgcattg
    32041 gtcgttttga actggcggat aaaagctctc tgttcctcga cgaagtgggc gatatgccac
    32101 tggagttaca gccgaagttg ctgcgtgtat tgcaggaaca ggagtttgaa cgtctcggca
    32161 gcaacaaaat cattcagacg gacgtacgtt taattgccgc gactaaccgc gatctgaaaa
    32221 aaatggtcgc cgaccgagag ttccgtagcg atctctatta ccgcctgaac gtattcccga
    32281 ttcacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg aaagccttta
    32341 ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattcctgcc gagacgctgc
    32401 gcaccttgag caacatggag tggccgggta acgtacgcga actggaaaac gtcattgagc
    32461 gcgcagtatt gctaacacgc ggcaacgtgc tgcagctgtc attgccagat attgctttac
    32521 cggagcctga aacgccgcct gccgcaacgg ttgtcgccca ggagggcgaa gatgaatatc
    32581 agttgattgt gcgcgtgctg aaagaaacca acagcgtggt tgccgggcct aaaggcgctg
    32641 cgcaacgtct ggggctgaaa cgcacgaccc tgctgtcacg gatgaagcgg ctgggaattg
    32701 ataaatcggc attgatttaa ctgcaaattg ccggacagat ctgcctgtcc ggcatactat
    32761 tcacgaggtt ttttcggacg atatttttcc ggcagttctg gcaccggacg cttgtcatcg
    32821 atgagatgac gcacggttaa gatcggatga cgccacagca ttctcggccc ggcccaacgc
    32881 ataatctgtt tcatctcttc acgctttgca ggctggtaa
//
LOCUS       DAJVPP010000041        32260 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVPP010000041 DAJVPP010000000
VERSION     DAJVPP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.041"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(5..541)
                     /gene="ssb1"
                     /locus_tag="OQM34_001868"
     CDS             complement(5..541)
                     /gene="ssb1"
                     /locus_tag="OQM34_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1452344.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            795..3617
                     /gene="uvrA"
                     /locus_tag="OQM34_001869"
     CDS             795..3617
                     /gene="uvrA"
                     /locus_tag="OQM34_001869"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1452345.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3652..4008)
                     /locus_tag="OQM34_001870"
     CDS             complement(3652..4008)
                     /locus_tag="OQM34_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1452346.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4012..4428)
                     /locus_tag="OQM34_001871"
     CDS             complement(4012..4428)
                     /locus_tag="OQM34_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1452347.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4539..5252)
                     /gene="aphA"
                     /locus_tag="OQM34_001872"
     CDS             complement(4539..5252)
                     /gene="aphA"
                     /locus_tag="OQM34_001872"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1452348.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5654..5858
                     /locus_tag="OQM34_001873"
                     /pseudo
     CDS             5654..5858
                     /locus_tag="OQM34_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6361..7554)
                     /gene="tyrB"
                     /locus_tag="OQM34_001874"
     CDS             complement(6361..7554)
                     /gene="tyrB"
                     /locus_tag="OQM34_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1452349.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7807..8886)
                     /gene="alr"
                     /locus_tag="OQM34_001875"
     CDS             complement(7807..8886)
                     /gene="alr"
                     /locus_tag="OQM34_001875"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1452350.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(8939..10354)
                     /gene="dnaB"
                     /locus_tag="OQM34_001876"
     CDS             complement(8939..10354)
                     /gene="dnaB"
                     /locus_tag="OQM34_001876"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1452351.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10437..11420
                     /locus_tag="OQM34_001877"
     CDS             10437..11420
                     /locus_tag="OQM34_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1452352.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQCAHEILESRATQGSSLLIP"
     gene            complement(11586..11828)
                     /gene="pspG"
                     /locus_tag="OQM34_001878"
     CDS             complement(11586..11828)
                     /gene="pspG"
                     /locus_tag="OQM34_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1452353.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(11962..12999)
                     /gene="dusA"
                     /locus_tag="OQM34_001879"
     CDS             complement(11962..12999)
                     /gene="dusA"
                     /locus_tag="OQM34_001879"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1452354.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13362..14347)
                     /locus_tag="OQM34_001880"
                     /pseudo
     CDS             complement(13362..14347)
                     /locus_tag="OQM34_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14665..15180
                     /gene="zur"
                     /locus_tag="OQM34_001881"
     CDS             14665..15180
                     /gene="zur"
                     /locus_tag="OQM34_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1452355.1"
                     /translation="MGKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15222..15431)
                     /locus_tag="OQM34_001882"
     CDS             complement(15222..15431)
                     /locus_tag="OQM34_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1452356.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15547..16872)
                     /gene="dinF"
                     /locus_tag="OQM34_001883"
     CDS             complement(15547..16872)
                     /gene="dinF"
                     /locus_tag="OQM34_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1452357.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSIAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(16945..17553)
                     /gene="lexA"
                     /locus_tag="OQM34_001884"
     CDS             complement(16945..17553)
                     /gene="lexA"
                     /locus_tag="OQM34_001884"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1452358.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17663..18031)
                     /gene="dgkA"
                     /locus_tag="OQM34_001885"
     CDS             complement(17663..18031)
                     /gene="dgkA"
                     /locus_tag="OQM34_001885"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1452359.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18202..20625
                     /gene="plsB"
                     /locus_tag="OQM34_001886"
     CDS             18202..20625
                     /gene="plsB"
                     /locus_tag="OQM34_001886"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1452360.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20780..21652)
                     /gene="ubiA"
                     /locus_tag="OQM34_001887"
     CDS             complement(20780..21652)
                     /gene="ubiA"
                     /locus_tag="OQM34_001887"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1452361.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21665..22162)
                     /gene="ubiC"
                     /locus_tag="OQM34_001888"
     CDS             complement(21665..22162)
                     /gene="ubiC"
                     /locus_tag="OQM34_001888"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1452362.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22385..23965)
                     /locus_tag="OQM34_001889"
                     /pseudo
     CDS             complement(22385..23965)
                     /locus_tag="OQM34_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24194..25114)
                     /gene="malM"
                     /locus_tag="OQM34_001890"
     CDS             complement(24194..25114)
                     /gene="malM"
                     /locus_tag="OQM34_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCS1452363.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSRTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25266..26606)
                     /gene="lamB"
                     /locus_tag="OQM34_001891"
     CDS             complement(25266..26606)
                     /gene="lamB"
                     /locus_tag="OQM34_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCS1452364.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26678..27793)
                     /gene="malK"
                     /locus_tag="OQM34_001892"
     CDS             complement(26678..27793)
                     /gene="malK"
                     /locus_tag="OQM34_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCS1452365.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28158..29348
                     /gene="malE"
                     /locus_tag="OQM34_001893"
     CDS             28158..29348
                     /gene="malE"
                     /locus_tag="OQM34_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCS1452366.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29502..31046
                     /gene="malF"
                     /locus_tag="OQM34_001894"
     CDS             29502..31046
                     /gene="malF"
                     /locus_tag="OQM34_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCS1452367.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31061..31951
                     /gene="malG"
                     /locus_tag="OQM34_001895"
     CDS             31061..31951
                     /gene="malG"
                     /locus_tag="OQM34_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCS1452368.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8211 a         8272 c         7799 g         7978 t
ORIGIN      
        1 caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg ctcgttagac ggcgctgccg
       61 gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa ctgattgcca ccctgcggct
      121 gctgaggctg accccaaccg ccctgcggct gaccaccacc gatattgcca cctgccggag
      181 cgccaccacc ctgacgacca cccagcatct gcatggtgcc gccaacgttc accacgactt
      241 ctgtggtgta gcgatcctga ccggattgat cggtccattt acgggtacgc agctgacctt
      301 cgatataaac ctgagaacct ttacgcagat attcgctcgc cacttctgcc agtttgccga
      361 acagcacaac gcggtgccat tcagtctgtt ctttcatctc gccggtcgct ttatcacgcc
      421 aggattcgga agtagccagc gtaatgttgg caactgcgcc accatttggc atgtagcgta
      481 cttccgggtc ctgacccaga ttaccaacga gaataacctt gtttacgcct ctgctggcca
      541 tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta aacgcgcgat tgtaccatta
      601 ccaatagcgc ttttactatg ttgtgacctc ggttccggga aacaaacctg gccagacatt
      661 gttacacaac actccggata atgcattcca atactgtata ttcattcagg tcaatttgtg
      721 tcataattaa ccgtttgtga tcgccggtag caccatgcca ccgggcaaaa aagcgtttaa
      781 tccgggaaag gtgaatggat aagatcgaag ttcggggcgc ccgcacccat aatctcaaaa
      841 acatcaacct cgttatcccc cgcgacaagc tcattgtcgt gaccgggctt tcgggttctg
      901 gcaaatcctc gctcgctttc gacaccttat atgccgaagg gcagcgccgt tacgttgaat
      961 ccctttccgc ctacgcgcgg cagtttctgt cactgatgga aaagccggac gtcgatcata
     1021 ttgaggggct ttctcctgcc atctcaattg agcagaaatc gacgtctcat aacccgcgtt
     1081 ctacggtggg gacaatcacc gaaatccacg actatttgcg tttgttgttc gcccgcgtcg
     1141 gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc gcaaaccgtc agccagatgg
     1201 tggataacgt gctgtcgcag ccggaaggca agcgtctgat gctgctcgcg ccaatcatta
     1261 aagagcgcaa aggcgaacac accaaaacgc tggagaacct ggcaagccag ggttacatcc
     1321 gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc gccgaaactg gaactgcaaa
     1381 agaaacatac cattgaagtg gtggttgatc gcttcaaggt gcgtgacgat cttacccaac
     1441 gtcttgcgga gtcgtttgaa accgcgctgg aactttccgg tggtacagcg gtagtggcgg
     1501 atatggacga cccgaaagcg gaagagctgc tgttctccgc caacttcgcc tgcccaattt
     1561 gcggctacag tatgcgtgaa ctggagccgc gactgttttc gtttaacaac ccggcaggtg
     1621 cctgcccgac ctgtgacggc cttggcgtac agcaatattt cgatcctgac cgcgtgatcc
     1681 aaaaccccga gctgtcactg gctggcggtg cgatccgtgg ctgggatcgc cgcaacttct
     1741 attacttcca gatgctgaaa tcgctggcag atcactataa gttcgacgtc gaagcgccgt
     1801 ggggcagcct gagcgcgaac gtgcataaag tggtgttgta cggttctggc aaagaaaaca
     1861 ttgaattcaa atacatgaac gatcgtggcg atacctccat ccgtcgtcat ccgttcgaag
     1921 gcgtgctgca caatatggag cgccgttata aagagacaga atccagtgcg gtacgtgaag
     1981 aattagccaa gtttatcagc aatcgcccat gcgccagctg cgaagggacg cgtctgcgtc
     2041 gggaagcgcg ccacgtttat gtcgagaata cgccgctgcc tgctatctcc gacatgagca
     2101 tcggtcatgc gatggaattc ttcaacaatc tcaaactcgc tggtcagcgg gcgaagattg
     2161 cggaaaaaat tcttaaagag atcggcgatc gcctgaaatt cctcgtaaac gtcggcctga
     2221 attacctgac actttcccgc tcggcagaaa cactttccgg cggtgaagcc cagcgtatcc
     2281 gtctggcgag ccagattggt gcgggcctgg ttggcgttat gtacgtgctg gacgagccgt
     2341 ctatcggcct gcaccagcgc gataacgagc gcctgttggg tacgcttatc catctgcgcg
     2401 atctcggtaa taccgtgatt gtggtggagc atgacgaaga cgcaattcgc gccgctgacc
     2461 atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg tgaagtggtc gcagaaggtc
     2521 cgctggaagc gattatggcg gtgcctgaat cgttgaccgg gcagtacatg agcggtaaac
     2581 gcaagattga agtgccgaag aaacgcgttc cggcgaatcc agaaaaagtg ctgaagctga
     2641 caggcgcacg cggcaacaac ctgaaggacg tgacgctcac gctgccagtc ggtctgttta
     2701 cctgcatcac aggggtttca ggttccggta aatcgacgct gattaacgac acactgttcc
     2761 cgattgccca acgccagttg aatggtgcga ccatcgccga accggcaccg tatcgcgata
     2821 ttcagggact ggagcatttc gataaagtga tcgatatcga ccaaagccca attggtcgta
     2881 ctccacgttc taacccggcg acctataccg gcgtgtttac gcctgtgcgc gaactgtttg
     2941 cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg acgtttcagc tttaacgtcc
     3001 gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat caaagtggag atgcacttcc
     3061 tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa acgctataac cgtgaaacgc
     3121 tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct ggatatgacc atcgaagagg
     3181 cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa gctgcaaacg ttgatggacg
     3241 ttggtctgac gtacattcgc ctggggcagt ccgcaaccac actttctggt ggtgaagccc
     3301 agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac cgggcagacg ctgtatattc
     3361 tcgacgagcc gaccaccggt ctgcacttcg ccgatattca gcaactcctc gacgtgctgc
     3421 ataaactgcg cgatcagggc aataccattg tggtgattga gcacaatctc gacgtgatca
     3481 aaaccgctga ctggattgtc gacctgggac cagaaggcgg cagtggcggc ggcgaaatcc
     3541 tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc ttcgcatacg gcacgcttcc
     3601 tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg gccttttcct ttcagagttg
     3661 taccagcaat ttacgctttt cttccggtag taaattcact gcctgctgat aagacgcatc
     3721 caccagataa tagatttgcg aatccggcag cgaaccgtcg agatacacgg tgctccagtg
     3781 cgctttattc agatggcggc taggacgcac atcgctgtgc tgctgacgta gcagctccgc
     3841 cagctccggg ctggttttca gcgaaacagc tgggcgattt tctacttctt tcaccatcgc
     3901 aaacagtaca tcttccactt tgatctgcgt cgctttccag tcgttatgca cgctctgttc
     3961 tgcgcttggt tttgccatgc aatattgtag caactccgaa atggtcattt tttactcccc
     4021 ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg cgatgttccc ccagccagat
     4081 gccttgccaa gtgccggtct gaatacgccc tttatgtacc ggcaatacaa gcgatgttcc
     4141 cagcattgag gatttgatat gagaaggcat atcgtctgct ccctcatagt catgctcata
     4201 atttccgttg tcgggaacgg tgcggaggaa aaaacactcc atgtcgtggc gtacggtggg
     4261 atcgcagttc tcattaagtg tcagagaggc ggaggtatgt tgcagcaaca gatgcagtaa
     4321 gccgatgtta acgcgcggca tatcagccag ctgattcaga atttcatccg ttaccagatg
     4381 aaacccacga gatttggcgc taagcgtgag cgtcttttga taccacatgt gctgctcctt
     4441 gataaaactc tcttaatcag tttgcagcaa gacagcgaaa ggataaaggt gtgattaaaa
     4501 aaacagcatt cgggagagca tcactctctc ccgctctgtc agtattctga attgacgatc
     4561 acctcttcac caaacgcacc cgcttgtggc aagggtttgt aggtagagtt ggaggcgcgc
     4621 agaatgcgga taccacgagc gccgacatcg cgtgcggcgg taatatcgtt atcggaatcg
     4681 ccataaaaaa ttcggatatt tttatcctgc agccattgcg attttgtatt ttgccctggt
     4741 ttatcgcccg caaagattac cggattcatg ttggtggcag gaatatgaaa attatccgcc
     4801 agcgtttttg aaaccgtttc tgttttcgtc gggctacgac cagtcacaaa gaagatcgcg
     4861 tcaccgcggc gtacatgcat atcaatcagc tggcgagcga cctcttttgg aatgctgaat
     4921 tcatcccagc cattattcat tttttcccag aacacaggat ttttcagata atcttcgctt
     4981 tctggcgaga aggttttttt gccgcgccag aaccccggac tggaaaaaag taccgtgtca
     5041 tcgatatcaa accccaccgc cattggcgga cgccctgcga ggctattttc aatttgtgcg
     5101 accgaaaccc aatgaattgg tgcctgttca gcaagcctgg caacgttagt cccagggtta
     5161 agcggtgaag gagatgaggc cagggcaaca gcggaactgt ttagagcgaa caataagcaa
     5221 acggcactga ttgcctgtgt gatcttgcgc atatttttcc ctaaatagtc agtatgttga
     5281 aactttttat tgtgagattt gttgcaaaaa ccatctgacc ataacgccag ctgccgggga
     5341 aaggaaggat tatctgcggt ttttgtgatg aaaagcagga tgattatatt taacccagta
     5401 aatacgttcc tgtctttttt tgtataaaaa atgtttatta ctcgatgtaa ggatacatta
     5461 attttacgtt tattatgatg aatcctcggc aaatgagcaa aatttgatag aacatttatc
     5521 tgctgtacga tttaatgaac ttaatgatac agcattacaa aaacaatcga agtttataaa
     5581 gatgatttct gattgaccag ccccctaccc tacaatggtg attaatttta ttactcgcga
     5641 tggagatgat gatatgaata tatcatatgt gaatagcaac aaaacgacat ctttacctgt
     5701 tgagctagat gcactaaata ataaagatat ctcttatgcc aaagatttct ttttatacat
     5761 agaaacacag ttaaaaattg caaaagattt ttgtagacct ggagaagaag tatcaagttc
     5821 tattgcaagt aaagtttttc acgcatttat tgatttagtt aacaaaatca ggggtaagaa
     5881 agactttatg tatatttgca cgctttgctg ttttgccgag gaggttaaag gtgattattc
     5941 tcattacagg acctttttat ttgatattgg taatcaatat aaggttaaac ttacacaaag
     6001 cggaaaaaag agttctcttt aacattagaa tttaacgata ctataattga atctcagaaa
     6061 gtcacaggca ataaggcaaa gcatattctt gaggatatag aaatattcta tcgtaacaaa
     6121 cccgatactt attattaaat ttaataatgg ttagatgtgc agaaattatt actccacacg
     6181 aaaaaatagg gaaaggaatt ccagataatt gggataaact taacaataaa agaactgaca
     6241 acgttttatt atctctcacg tagaacaacg gcatcaaaaa aatcatctac aaagaaatag
     6301 tgaaagcgac gacagtttaa tttcactgca ggctgggtaa ctccagcctg ctttcctgca
     6361 ttacatcacc gcagcaaacg cctttgccac acgttgtaca tttgccgtat ttaacccggc
     6421 gacacacatg cgaccgctgg cgatgagata gacaccaaat tcttcacgta gtcggtcaac
     6481 ctgagcggca cttaaaccgg tataactgaa catgccgcgc tgattaagca gataatcgaa
     6541 attgcgttct ggcatctctg tgcttaatac cttcaccaat tcctgacgca ttgccagaat
     6601 gcgagtacgc atctcttcta cttccgccag ccagctggct ttcaatgcct cgtcattcag
     6661 cactgcagcc accacctgcg caccaaaatt cggcgggctg gagtagttgc ggcgaactgt
     6721 tgctttcaat tgccccagta cgcggccagc ggcttcggca tcttcacaca gaacagaaag
     6781 tccgccgacg cgctcgccgt aaagggagaa aattttcgag aacgaattgc tcaccagagc
     6841 gggtaatcca gcgctggcaa tggcgcgaat agcgtaggca tcctcttcca taccggcact
     6901 aaatccttga taggcaatat cgaggaatgg gataagctcg cgggctttga gaatttcaat
     6961 caccgcatcc cactgatcat tagtgagatc ggcacccgtt gggttgtggc aacatggatg
     7021 cagcaacaca atactgcggg caggtaatgt tttcagcgtc gccaacaggt cattaaagcg
     7081 cacgctgtta gtcacttcgt cataccaggg gtaagtactc acttcgaatc cagccccggc
     7141 gaatattgct acgtggtttt cccaagtagg atcgctgacc cagacgcctg attccgggaa
     7201 gtagcgtttc aggaaatccg cgcccacttt caatgcccct gagccgccaa gggtttgaat
     7261 ggttgctacg cgctgttgtt gcagtaccgg atggtccgca ccaaacagca gcggcgcaat
     7321 ggcatggcga tagctgttaa gcccttccat cggtaaataa agcgaagcgc catggggctg
     7381 cgcattcagg cgcgcttccg cctccgccac ggctttcaat tgtggaataa ttccgtcttc
     7441 gttgtagtac agaccgatac ttaaattcac tttgtcgctg cgagggtctt ctttaaaacg
     7501 ctccataagc gtaagaatcg ggtcgccagc gtaggcgtca actttttgaa acacgcgatg
     7561 gttctccagg tttacgggca ggtggttaaa acacaataaa ccggaagaag gcgaagatcg
     7621 agtggatgtt caggagcgaa cggcaattag caacagagtg agactaatga taaacgtaca
     7681 tccgccagag gcacgacctt tataagagca tgaaagaaca tcaacttatt gaatttttag
     7741 gattttattg gccggataag gcattcacgc cgcatccggc acagacaatc aaatattaca
     7801 gaacgattaa tccacgtatt tcatcgcgac ccttgaagtc aggcgcgtaa taagttcgta
     7861 agcgcttact ttcgtcattt cagcgatacg ttctacgggc aaaccttcgc cccataaaat
     7921 gaccggatcc ccggctttgt cctgcgcctg tggacctaag tctacgcaga tcatatccat
     7981 cgccacgcgc ccgacaatcg gtacttcgcg accgttcacc agcactggcg taccggacgg
     8041 cgcggcgcgc ggataaccat cgccatagcc catcgcgact acgccaagac gggtatcacg
     8101 ttcgcttacc caggttccac cataaccaac aggctctccg gctttatgct cacgcacggc
     8161 aatcaggctg gaggttagtg acatcactgg ctgacagcca aaatcggcac cggtggagcg
     8221 atcttccagc ggcgagacgc cataaagaat gatgcccggg cgcacccagt caaaatgcga
     8281 ctgtggccac agcagaatgc cacccgacgc ggcaatggaa cgttgaccag gtttgccttc
     8341 gcaaaaggta ttaaagatag cgagttgttt ctcggttgcg ccacattttg gttcatccgc
     8401 gcgcgcaaaa tggctgacga tattcaccgg ctgacgaacg tttttgcact gggtcaggcg
     8461 atgataaaac gcctcagcct gttccggcct tacgcccaga cggtgcatac cggtatcgag
     8521 tttcatccag acggtaaccg gctcgtccaa gctagcctct tccagcgcag ccagctgttc
     8581 ttcgttatgc acggcggtat gaaaatgttg cgcagaaatc gtcggcagat ctctggcatc
     8641 aaaaaagcct tcgagtaaca gtacaggttt ggtgattccc cccgcacgca gtcgcagagc
     8701 ttcttcgaga cgggctacgc caaaggcgtc agcatcgggg agcgttcgcg cggtctcaag
     8761 aagaccgtga ccataagcgt tcgctttcac caccgcaacc attttactgg caggtgccag
     8821 ttcacgaaga cgttgcaggt tgtgtcgcag agcgcggcgg ttaatcacaa cagttgccgc
     8881 ttgcatttgt gttccttgat aattgtttgc tttaattacc taattcataa aataattatt
     8941 attcgtcgtc gtactgcggc cccgcatagt tgtcgaagcg cgaccattga ccgttaaagg
     9001 tcaggcgtac cgtcccgatt gggccgttac gttgtttacc gataataatt tccgcgatgc
     9061 cttttaaatc actgttttcg tgatacacct catcacgata gataaacatg atcaagtccg
     9121 catcctgctc gatagagcca gattcacgca ggtcggagtt gaccggacgt ttgtcggcac
     9181 gttgttccag agaacggttc aactgggaca gcgccaccac cggcacgttc agttctttcg
     9241 ccagtgcttt cagcgagcgg gagatttctg caatttccag cgtacggtta tcggaaagcg
     9301 ccggtacgcg catcagttgc aggtagtcga tcatgataag cccgatgccg ccgtgttcac
     9361 gggcaatacg gcgtgcgcgg gaacgcactt ccgttggcgt caggccggaa gagtcatcga
     9421 tatagatatt gcgtttttcg agcaaaatac ccatggtgcc ggaaatgcgc gcccagtctt
     9481 catcatcgag ctgcccggta cggattttag tctggtcaac gcgcgacagc gacgccagag
     9541 aacgcatcat gatctgttct gatggcatct ccagcgagaa gataagtact ggtttatcct
     9601 gcaacattgc cgcgttttcg acgaggttca tcgcaaatgt tgttttaccc atcgacggac
     9661 gcgcggcgac gatgatcaaa tccgacggct gcaagccagc ggtttttttg ttgagatcgt
     9721 cataaccggt gtttaccccg gtaacgccat cgtgtggctg ctgaaacaac tgctcaatac
     9781 gcgccacggt tgcgtcgagc acatcggcga tgttcttcgg cccttcgtct ttgtttgcac
     9841 gactttcggc aattttaaag acgcgggatt cagcaaggtc cagcagatct tcgctggtac
     9901 gcccctgcgg atcaaaaccg gcttcagcaa tctcattcgc aaccgagatc atctcacgga
     9961 caacggcacg ttcacgcacg atgtccgcat aagcactgat gttcgccgca cttggcgtat
    10021 tttttgacag ctctgccaga taagcaaaac caccgacgct atcgagttgc ccctggcgtt
    10081 ccaacgattc cgcaagggta atcagatcga taggactacc gctttcctgc aaacgcgcca
    10141 tttcagtaaa gatatgacgg tgtgggcggg tgtaaaaatc gtctgctacc acacgctcgg
    10201 ctacatcatc ccagcgttcg ttatccagca ttaaaccgcc caacaccgac tgctccgctt
    10261 cgatcgagtg cggaggcact ttcagcccgg caacttgtgg atcgcgttcg cggggttcag
    10321 cctgctgttt gttgaagggt ttatttcctg ccatagtgaa tggagttacc gaaataaaga
    10381 atgggtcgaa actttaccat atgaagcaga ccctgacgat acgttctgga ggacacatgg
    10441 caacacgaat tgaatttcac aagcacggtg gcccggaagt acttcaagcc gtagagttca
    10501 ctcctgccga tccggcggag aatgaaatcc aggtcgaaaa taaagccatt ggcatcaatt
    10561 ttatcgacac gtatatccgc agcggccttt acccgccgcc atcgctaccc agcggattag
    10621 gtaccgaagc ggcaggcatc gtgagtaaag tcggcagtgg tgtaaagcat attaaggctg
    10681 gcgatcatgt agtctatgcg cagtcggcgt taggcgctta cagctctgtg cataacatta
    10741 atgcggataa agcggcgatt ctgcctgcgg caatttcttt tgagcaagct gcggcatcct
    10801 tcctgaaagg cttaacggtt tattatctgc tgcgcaaaac ctatgaaatt aaacccgatg
    10861 aacagttcct gttccacgca gcggctggcg gcattggctt aattgcctgc cagtgggcaa
    10921 aagccctggg cgcgaaactc atcggcaccg taggaaccgc gcaaaaagcg cagagcgcgc
    10981 taaaagccgg cgcgtggcag gttattaact atcgtgaaga gaatctggtc gagcgattga
    11041 aagagatcac cggcggtaag aaagtgcgcg tagtgtacga ttccgtgggc agagacacct
    11101 gggaacggtc gctggattgc ctgcaacgcc gcggcttaat ggtcagtttt ggcaactcat
    11161 caggtgcggt taccggtgtg aacttaggca ttctcaatca aaaaggctcg ttgtatgtga
    11221 cacgcccttc cctgcaaggc tatatcacca cgcgggagga attaaccgag gccagtaatg
    11281 aactgttctc tttgattgcc agcggtgtga ttaaggtcga tgtcgccgag cagcagaaat
    11341 atccgctgaa ggatgcgcag tgtgcgcatg agattctgga aagccgggcg acgcaaggtt
    11401 ccagcctgct gattccataa aagaaatagg gcttccacct gggaagccct ttctttttat
    11461 agttcggctg tatgtagggt acagcacgat gaatctgtta gaggcgcaat agtgacagat
    11521 ttgattatca attcctattt tgttctaagg ataaaacctt aggttgtgat catccgcaca
    11581 atcccttagt aacgccagcg gtcataacgc tgatatttcg gcatttttgg tgctttaatc
    11641 gccttaataa cccacaccac cgcaatcgcc agtagtaacc acggcagcaa cttaatcatc
    11701 aatgccagca taccgccgag gaacataatg gccgtcgcca caaccagcgc ggcgataatg
    11761 cccagcaacg aaacgccggt gaccatcagc atgacaaaaa agccaatcac aaaaagtagt
    11821 tccagcatga tgctctccca aatatgaaat ctcttgctgg cattacaaga atcatgccaa
    11881 aaataatcta ttgatttaac agcaaaacgc cccgcgacgg tgcgcagggc gtggtgaatt
    11941 tgactacttt ttggtgaaaa gttaacgctt atccgccacc agtttgagcg cgtgttccag
    12001 cacgttaatg tcagcacccg ctttatgggc attttcactt aaataacgcc gccactgccg
    12061 cgcgccagga ataccctgga acaaacccag catatgccgg ataatatggc cgagatacgt
    12121 cccctggctg agttcatgct caatgtacgg atacatggca cgcactactg ccaccggatc
    12181 ggcatcggta tccgaggaac caaagatctc ccggtctacc gccgccagaa tacccggatt
    12241 ctgatacgcc tcgcgcccga ccatcacgcc atccatatgt tgcaaatgcg ctttagcttc
    12301 ttccagcgac ttgataccac cgttaatcga cattgtcaga tgcgggaagt cacgcttcag
    12361 ttgatacaca cgcggataat cgagcggcgg gatttcacgg ttttctttcg ggcttaaccc
    12421 cgaaagccag gctttacgtg cgtggatgat gaacatctca cactcgcctt tgccggaaac
    12481 ggtattgatg aaatcgcaga gaaattcata gctgtcctgg tcgtcgatgc caatacgcgt
    12541 tttcaccgtc accggaatcg acaccacatc gcgcatcgct ttcacgcagt cggcaaccag
    12601 ctgcgcatta cccatcagac acgcaccaaa catgccgttc tgcacccggt cagacgggca
    12661 gccgacattc aggttgatct catcatatcc gcgcgcttct gccagctttg cacactgcgc
    12721 cagcgccgcc ggatcgctac cgcccagttg caacgctacc ggatgttctt cttcactgta
    12781 cgccagataa tcacctttac cgtgaataat cgcccctgtg gtcaccattt cggtataaag
    12841 caacgtattg cgggaaagca gacgcaagaa atagcggcaa tgtctgtccg tccagtcgag
    12901 cattggtgca acactaaacc gaccactcca gtgaacgtca gttttttcag gcattgcgct
    12961 ggtttggttg attttttgca tttcagaatt accgtgcatt ttcaaatgta gagattattt
    13021 tatcgatata tcatggggtt atgttattca gcttcactgt tcaggaggct caatagcggg
    13081 gtactatacc ataacaacag gaagcgcctg tctcattgca aaagaaaatt gagatcctct
    13141 caaggcatga agctctcacg aagtgatgga aataatctta ttagccgtta actttgttaa
    13201 ggccaatgat aaacaatcca ggttcgacga taaataaaaa atcacacatt aaactctggt
    13261 gatatatctc cctgctaata gcattgatag agaaaaaaga acccaataaa tattgggtcc
    13321 ttttatataa tgccttccat actatcgaag aacttcacat attatttctc cgatttaacc
    13381 ccgaacaagt cataaattaa tttagaagtg tctgtaagta ttttaatctc ttcctttgag
    13441 gttgggtcaa acgacttcgc gaagctaatt aattgtggtg cagcatctcg cattttgctt
    13501 aaaatatcag gatcgagcgt tccttcattc accagatgtg acatcttatc cagatagcca
    13561 tcaaaattca tttctccccc atctgccaga cgcttcatct ctcccaggta cttcttcata
    13621 tcacgttgat ataatttttc aaactgagct ttcaggtagc tctcattatt ttcattaata
    13681 tacactgttt ctgataaatc tgcgtaggca ctagaatatg aaagtgaaat taacagcgaa
    13741 gcaaataatt ttaagctgtt gtcatcatgt tcctggcagg cattaacaaa cgttaaaaaa
    13801 ccagaaccta ttgcttgaaa atgtatattc gctaaaggtt cattatcctt agattcctca
    13861 taaaatagct gaaccgtgga gggaaggcca ttcttagttt ctacatcaaa agtacattgt
    13921 tcaatgggca ttgcatcttt attatcccca ggattacata tactattaat cgcgaaatga
    13981 aaaatagccc ggtcaacaac tgatgcaata aagtcacgat cgttcaaatc ctcatcagtg
    14041 caatcgtcag tatacttatt gacccacatt ttgagatcat ttatgtgctc aattaaaaac
    14101 acattttttt acgtgtatgc cataaattat gcgtgattcg ttgaataaac aaatctattt
    14161 ttgcaataat tatatcccga tgacgcagcg ctgcataatc aatagattta ccatatatat
    14221 tatttaattt acctttgatt gaacctctat tatccaattt atctttggtt gcatctttat
    14281 tttcgaattg atttgaattg agtgttttat taaagtatcc agttgcaccg ggtattatct
    14341 tcaacatgat tcatctacct tttttgtcgt accgtgtcct ccttataatt cttttgccat
    14401 ctggtcacta tcaaaaatcg ctcataattt ctgaactaca ggtatgcgtt taaacccagt
    14461 taaataaata gcgagttaac ttgaagcaat ttatttaaaa atgaattatt aattattatc
    14521 gtgttgataa tggtacgaca aggcaacaca acaccttacc tacaaaacag aaaatcaaca
    14581 ttacggcaac aataagggtt ctcggtgttt ttcctggaac atggtaaagt aaggacattc
    14641 ttaaccccca ctttgaggtg cccgatggga aagaccacaa cgcaggagtt attagcgcag
    14701 gctgaaaaaa tctgcgcgca gcgtaatgtg cgcctgaccc cacagcgcct ggaagtgttg
    14761 cgcctgatga gtctgcagga tggcgctatc agcgcttatg atctgcttga tttactgcgc
    14821 gaagctgaac cgcaagccaa gccgccaacg gtttatcgcg cgctggattt tctgcttgag
    14881 caaggttttg tgcataaggt ggaatccacc aacagttatg tgctctgtca tctgttcgac
    14941 cagcccacgc atacgtcagc catgtttatt tgcgatcgct gcggcgcggt gaaagaagag
    15001 tgtgcagaag gcgtggaaga cattatgcat acgctggcgg caaaaatggg gtttgccctg
    15061 cggcataatg tgattgaagc acatgggctc tgtgcggcat gcgtggaagt ggaagcgtgt
    15121 cgtcatcctg aacagtgcca gcatgatcac tctgtgcagg tgaaaaagaa accgcgttaa
    15181 gagggcgtac atccttgtac acgtcgggca ggagggatta attaccagcg atattcattg
    15241 cgggtttccc aatccacgac ctctttttct gcctgatctt tctgataacc ataacgttcc
    15301 tggattttac cgaccagttg atcacgttta ccttcaatga tcgtcatatc atcatcggtc
    15361 agtttgcccc attgctcttt cactttacct ttaaactgtt tccagttacc gccggcttca
    15421 tctttattca taatcaagac ctcatcgtta ggttgtgaat gagagtacgt tcacttttct
    15481 tctgaacgtg agattaagtg tagtcagcaa tttggcttag gataatttat tcagaatttt
    15541 taaccgtcac gttgcggcaa accaggtacc gttgcgccag tgacgccgcc agatagccgc
    15601 cagagaaagc ccgcgcaacg ccagaaagac ggttaatgcc agccacaaac catgattacc
    15661 cagccacggc agcgtaagga gcgtcagcgc aaaacctgcg gcggccaccg ctatactgtt
    15721 acgcatttcg gcggcacgcg ttgcgcctat aaacatgccg tccagcagat aacaccagac
    15781 gccaaccaac ggcaaaatca cctgccagat aagatagcgg tcagccagct gctgaatctg
    15841 ggttaacgac gtcagcaacg caatgatgtg ttcccctgcc agcaaataaa ccaccgaaaa
    15901 cagtaacgct acaatccccg actggcggca cgctgcccgc catacatcca gcaactggct
    15961 accgtcgcgc gcaccgtatg cctgaccgga gtgcgcttca accgcgtagg caaaaccatc
    16021 aagcgcatag gcggtaaagg tgagtagcgt catcagaacc gcgttaacag cgataatgtc
    16081 actccccagt cgcgcaccaa gtacggtgat cgcgccgaaa cagagttgca acaacagcga
    16141 acgcagcatg atatcgcggt taagcgccag caagcgacgg aagtttcctc gccaggcagt
    16201 tttcagcatt tcgccggaaa ttccgcgtag tttgaggatt ttacgcacca ttagcagacc
    16261 aatcagcaat gttgcatatt ccgcaataac cgtcgccagc gccgcgccct gcacgttcat
    16321 atgcagcccc atcaccagcc agacatccag cacaatgttg aggatattgc cgaccactaa
    16381 caaaattact ggcgcacggg catattgcac gccgagtaac caaccgagta ataccagatt
    16441 cgccagcgac gccggagcgc ttaaccagcg gatttcaaga aagcgccgcg cctgttccag
    16501 gaccgcttca ctaccgccaa caatatgcag cgccagatcg ataatcggcg tacgcaacag
    16561 cgcaattaac gccccggccc ccaacgccaa cagcaacggt tgcaccagcg cacgggctaa
    16621 tgcctgagga tttttggcac cataagcctg cgcagtcagc ccggtggtgc tcatgcgtaa
    16681 aaacagcaac agcataaaga gaaagctggt cgccgttgca ccaaccgcca cgccgcccaa
    16741 ataaaccgga ctatcaagat gaccaattac cgccgtatcg accagtccca gcaacggaac
    16801 ggtgatattg gagaaaatca tgggtaaggc gagatgccag agtgctttat cagatgaagt
    16861 gaggaatgcc atgcagacaa gcctgatgaa gagagatgaa aaacaaaccg cgacgccaag
    16921 cggcatcgcg gtctcagaga tatgttacag ccagtcgccg ttgcgaataa ccccaaccgc
    16981 cagcccttca atggtgaagc tctgctgacg aaggtcaacg acaattggtt taaactcgct
    17041 attttctggc aacagttcga ctttattgcc ctgttttttc aggcgcttaa cggtaacttc
    17101 gtcatcaata cgtgcgacaa cgacctgacc gttacgtaca tcctgagttt tatgcactgc
    17161 cagcaagtcg ccatccataa tgccgatatc tttcatcgac atcccgctga cgcgcagcag
    17221 gaaatcagca ttcggcttga acaaggaagg atcgacctga taatgacctt caatatgctg
    17281 ttgcgccaga agcggttcac cggcagccac acgacctacc agcggcaacc cttcttcctc
    17341 ttcctgcaac agacgaatcc cgcgtgatgc gccggaaaca atttcaataa cgcctttgcg
    17401 tgccagcgcc ttcagatgtt cttcagccgc gtttggggaa cggaacccca aacgctgcgc
    17461 gatttccgca cgcgtcggcg gcatacctgt ctggctgatg tgatcacgga tgagatcaaa
    17521 cacctcttgt tgcctggccg ttaacgcttt cattccgccc cctgggtgta tatacagtta
    17581 tactgtgagt atatacagca aaaggcgatt ttggaaccat aaactgcaca ataaaccaga
    17641 gatttatcga aatctggaag ggttatccaa aatgcgacca taacaggatg caccaggtaa
    17701 tcacggcgac gataatggca atcagcaccg cggcggatcc catatctttt gcgcgtccgg
    17761 aaagctcatg gtattcagag ccaattcggt caaccactgc ttcgatggcg ctattgagga
    17821 tttccacaat catcaccagc atcacggagc tgataagcag cacgcgggta attgcgtcca
    17881 catccagcca gcaggcgatg accaccgcca acaataccgc tacgccttcc tgacggaatg
    17941 ccgcttcgtt gatccatgca gcgcgtaaac ctttccagga atagccagca gctttgataa
    18001 ttcgggtgaa tccagttgta ttattggcca ttgaaagaac ctttttacat tatgagcgtc
    18061 aatatcagtg taccgttaaa tccgatgatt cgggtttgcg taacgccgcg cgaaacatga
    18121 gcggatacca cagaatttcc catgactttc tgctatcctt gccgcgcatt tgcattatta
    18181 accagaggct ttacatcgtt tatgtccggc tggccacgaa tttactacaa attactgaat
    18241 ttaccattaa gcatcctggt aaaaagcaag tctattccgg cagatcctgc cccggaactg
    18301 gggctggata cctctcgtcc aattatgtac gttttaccgt acaactcgaa agcagatttg
    18361 ctgacgttgc gcgcccagtg tctggcacat gatttgcctg acccgttaga gccgctggaa
    18421 atcgacggca cgctactgcc gcgctatgtg ttcattcacg gcgggccgcg tgtattcacc
    18481 tattacacgc cgaaagaaga gtctattaag cttttccacg actatctcga tttgcaccgt
    18541 agcaacccaa atctggatgt gcagatggtg ccagtatcgg tgatgtttgg tcgcgcgccg
    18601 gggcgtgaaa aaggcgaagt gaacccgccg ctgcgtatgc ttaacggcgt acagaaattt
    18661 ttcgctgtac tgtggctcgg tcgcgacagt tttgtgcgtt tctcgccgtc agtgtcgctg
    18721 cgccgtatgg cggatgaaca cggcacggat aaaactatcg ctcagaaact ggcgcgcgtg
    18781 gcgcgtatgc actttgcccg tcaacgtctg gctgccgtag gcccacgtct gcctgctcgt
    18841 caggatctgt ttaataagct gctcgcctcc cgcgccattg ccaaagcggt agaagatgaa
    18901 gcgcgcagca aaaaaatctc ccatgaaaaa gcgcagcaga acgcgattgc gctgatggaa
    18961 gagattgcgg cgaatttctc ttacgagatg atccgcctga ctgaccgcat tctgggcttc
    19021 acctggaacc gattttatca gggcatcaac gtccataacg ccgagcgcgt tcgccagctg
    19081 gcccacgacg gtcatgagct ggtgtatgtg ccttgccacc gcagtcacat ggactacctg
    19141 ctactttctt acgtgctgta ccaccagggg ctggtgccgc cgcatatcgc cgccgggatc
    19201 aacctgaact tctggccggc cgggccgatt ttccgccgtc tgggggcgtt ctttattcgc
    19261 cgtactttta aaggcaataa actttattcc accgttttcc gtgagtatct cggcgaactg
    19321 ttcagccgtg gttattccgt cgagtacttc gtggaaggcg gtcgttcccg cacggggcgt
    19381 ttgctggatc cgaaaaccgg tacgctgtcg atgaccattc aggcgatgct gcgtggcggc
    19441 acgcgtccga ttacgctgat tccgatctat atcggttatg agcacgtcat ggaagtgggt
    19501 acttacgcca aagaactgcg cggcgcgacg aaagagaaag agagcctgcc gcagatgctg
    19561 cgcggtttaa gcaagctgcg taatctcggt cagggttacg tcaacttcgg tgaaccaatg
    19621 ccgttgatga cctaccttaa ccagcacgta ccagactggc gtgaatctat cgatcccatc
    19681 gaagcggtgc gtccggcctg gttaacgccg acggtcaata atattgctgc cgatctgatg
    19741 gtacgcatta acaacgcagg cgcggcaaac gccatgaacc tgtgctgtac tgcgctactg
    19801 gcatcacgtc agcgctcact cacccgcgag cagttaaccg agcaactcaa ctgctacctg
    19861 gatctgatgc gcaacgtacc ttactccacg gactctaccg ttccttcagc cagcgccagc
    19921 gagcttatcg atcacgcgct gcaaatgaac aagtttgaag tcgagaaaga cactatcggc
    19981 gacatcatca ttctgccacg cgagcaagcg gtgctgatga cctactatcg caacaacatt
    20041 gcgcatatgt tggtgctgcc ttcgctgatg gcggcaattg tcacccagca tcgccacatc
    20101 tcccgcgacg tattgatgga gcacgtcaat gtgctttacc caatgctgaa agcggagctg
    20161 ttcctgcgct gggatcgcga cgagttgccg gacgttattg atgcgctggc aaatgagatg
    20221 caacgtcagg ggctgattac cctgcaagat gatgagttgc atatcaaccc ggcgcattct
    20281 cgcaccctac agctgctggc cgcaggcgcg cgcgaaacgc tgcaacgtta tgccatcacc
    20341 ttctggttgt tgagtgccaa cccgtcgatc aaccgcggta cgctggagaa agagagccgc
    20401 accgttgcgc aacgtctctc cgtgctgcac ggcatcaacg cgccggagtt cttcgacaag
    20461 gcggtgttca gttctctggt gctgacgctg cgtgatgaag ggtatatcag cgatagcggc
    20521 gatgccgaac cagcagaaac gatgaaggtt tatcagttgc tggcggagtt gattacatca
    20581 gacgtgcgtt tgacgattga gagtgcgacg cagggcgaag ggtaatcaga gagaattgcc
    20641 ggatgcggcg aaaacgcctt atccggccta ccatgacctg caaattcaat aaattgcgat
    20701 tcaccgggta ggcctgagaa gcgcaacgca tcaggcaacc cagaagaaag ccggatgatc
    20761 atccggcttt tttacatcat cagaaatgcc agtaactcat tgccagtcct aaaaacagta
    20821 ccagaccaac atagttatta ttcataaatg ctttaaagca ggcttcacgc tcgcggttgg
    20881 caatcagttt ttgttgataa acaaacagcg cgccagccac cacaattgac cagtaatatc
    20941 cccagcctaa gccatttaac tcaccgatga tcgccatcag tgccagtacg ccaatctgca
    21001 aaataccaat aatcaattta tcgtattggc cgaacaggat tgccgtggat ttaatgccaa
    21061 tcttcacatc atcatcgcgg tcaaccatcg catactgcgt gtcgtaagcc accgcccaga
    21121 gaatattggc gaggaacatt aaccagcaac tcaatggcac cgactcactc acagcggcaa
    21181 aagccattgg aatcgaccag ccaaacgccg cgcccagcac cacttgcggg agatgggtat
    21241 accgcttcat aaacgggtac acccacgcca atgccagagc ggcaatcgac aacagaatgg
    21301 tcatcgtatt cagcgtcaga accagtaaaa acgaaatcag taccagcact acaaacagcg
    21361 cgcgcgcctc tttctctgtt accgcgccgc tgggaagtgg tcggttcgcc gtgcgcttaa
    21421 catgaccatc aaacttgcga tcagcataat cattcaccac gcaaccagca gcgcgcatca
    21481 gccagacacc cgcgacaaac accgccatga tccagagctg aggaacgccc ggtgtcgcca
    21541 cccacaacgc ccataatgtt ggccagagca gcagtaacgc gccaattggc ttatccgtac
    21601 gcattaagcg atgaaacgcc agcagcttat tctgcgtcag actccactcc atattttttt
    21661 cctcttagta caacggtgac gccggtaaaa acagttctgt tagcaacagc ggtttaccgc
    21721 ttaatcgcag gcgggaacgt cgcccccaca gtccggcatc acggcctatc tcgataaaat
    21781 cccgggttag tgtcgatgat gtgaacagat agcgtcctaa cggcgtttta cccaattttt
    21841 gtaacgctag ttccggcccg cttaatgttg atacaggaac gacggtacga ccggcaagcc
    21901 acggttcacc atcggcacat aacaaaattt cacgtaacca gtaacgagac tctttcggca
    21961 gcagcggcag ttcttcgggg atttcattct gctcgacaaa cccctcgcgg atcatcgtca
    22021 cactcaccgt ttttccctgc tgttcaaaac gttttgtcat ggaatcctcc agcaacagcc
    22081 agtcgagcag ttgcggatcc agggcaggga tctctttaaa atagcgcagc gcacgcagtt
    22141 gcgttaacgc ggggtgtgac atgccgaact ctccgttaca taacgtaaag gcattgtatc
    22201 gcagaaaagt gtttacggga gatgtaaagc aaattaagtg atctaatgcg caacaaaaac
    22261 gcaacaatcg catgaagtgg taaaacgctg ttgaacctta cgctttgccg atgcccgtgc
    22321 tggcgagggc aaccataggg gcatcaccgc ccatgatgct aggaacggtg atgttttcct
    22381 ttacttaaat acctttattc tttatacgag tagctcgcca actcgcagta aaaattggga
    22441 aaagcgcata tttaccatga gatttggagc tgaaaagtgt ttcctgagta agattaatag
    22501 gcaacatctt atgtctggat ttatagatac acgtggaatt ccaattggta tcttttgtag
    22561 gattcaacat atcatgtaag cgctgggagc ttaccaccat aacaggccca tcatcgacag
    22621 aggagaacag gataaatata ttatcgtttc gtgttaattt attagtgtta aaggttctta
    22681 acttttcttc agtttctata aatataggtg aaacatcatc actacgaatc cactctttat
    22741 aaagatacca ggcatttgct ttgatcatat catccgttga tcgtaaggcc tgatcgataa
    22801 gttggataaa aaaattgtta tttttatcaa ccagcccttt attcatatta aatctgttaa
    22861 taaaatggat catctgttgg gtatcgaaat tcaccttact gaatcgatgt tgacgcataa
    22921 aattatcgaa cttgtcatct tccccagaat gtatataact acgtataatt ggatctttta
    22981 tataaggaca atggctaaat gttttcttca tattcttaat aaagttttta ttaacaatat
    23041 tgagaatttt taattgcatc accagtttta ctgccagaac ggatttcaat atcattgcat
    23101 tatctggcaa tgtatcaatc atttttataa tgttattgat ataaccatcg agatcttcat
    23161 tcccaaatat atctgaataa tcaatagtta attccaattg ttctgtaatg caatcttaaa
    23221 aattccctgt cgacattcgc acatgatcaa acataacagc tgtcaaattc gatttaataa
    23281 atgatatttc tttggcagac agaaactctg ctctggtgaa gtgcgaagcg taaagataac
    23341 aattatcgaa gtttgaattt ctaatctgac attcgcgaaa attactatga ccaagttgac
    23401 tctgcgagaa gtcaactgta tcaagaatat tttggctaaa atcaacacat tcgccactcg
    23461 ttttgagaaa aatcacattg tgtaaacgtc gacatttaaa tatgacatta tcaactgttg
    23521 ccttagtaaa attgcaattc tcaatttctt tacattcgaa agttacatgt tgtagatttg
    23581 atgataaaaa agacacctta gaaataacgt cacctttaaa atgacaattt gttaaatcaa
    23641 aatcagacag gtcgatatac ccctgcgcat ctttatccat cagaaaatta caagcgcatt
    23701 ctattttttc atttctgtag aattttacac ttatagtatc attactaatt gagacttcac
    23761 gtaacatatt tattgcatga tttttttcgt tccgttgttt tatatttaaa aatatatcac
    23821 tgatataaat aaagtaatct ctgtttaaac ctgtcacccc attgaaatta cgtagtaaat
    23881 caagatctac tatattattt ccttgcacca catcatgttt catcaaagag ttattgccgc
    23941 cagaatttgt gatatgacct atcataaaac ctctgtaata cagaaataaa ataacctgga
    24001 aaagtagcct gagcgtccag acactaccgg cgaaaggaaa agaatctctg ataatgcatt
    24061 gaaataatgg acattcttag tgaagaagaa gtgaagtact ataaaaaatc gctcatatcc
    24121 caggaaggaa gcataaaaaa aggtgcgcca ggagacgcac cagttgttgc aaaatcagca
    24181 ctgtggggcg taattacccc ttgcctttta cactgctgat aaaggtggaa cgggcagatg
    24241 tcgatcccag gcgttcagct tcatcaagca gttttaacgc cttatcaaca tcacctttcg
    24301 cgacagcgtt tttgatcgcg gtattaaaat aactttccgt gtcgttgagc atcggctcgc
    24361 ttttcttcac cggcaccgga gcaggtgcag ccacagctgg tgccgccgtg ttgcctaccg
    24421 taaccggagc tggagcggaa gaaccaaata gtggtcctac caacacgctg gagctggagt
    24481 tcgttttgac tttcagtttc agtaagccat cgctggtatg acgagcaacc ggatcgggaa
    24541 tatccgggat cgagttaccg acgcctttgg cataggcttt agccgggtcg agcagttggg
    24601 tcgtctgctg gagatctttt tccgtggtaa agaccagaac ataaagtttt tgctgcccca
    24661 acgccggtgt caggcgcata acgccttcca gccgctctgc actcatcacg cctagttcct
    24721 ggtaggtgaa ataactgctg gggaagaagg ctgatggggt catgttctga tcaagaatca
    24781 gcacgttcgg cgcaaaaacg ctggtttgtt tgttcacttc gctggtcagc gtcaggggca
    24841 gttcgccaat gtttgccggg acgctgtacg cagcaaccgg accactgatg ccgggaacgt
    24901 tcagttgttg gccgccggtc gccagttggg tggtctgggt tctagattga tcgaccggtg
    24961 tccaggtgag ttgttgcagc gcggcagatg gaatggctgg cgcgtcgctg gtgttttgcg
    25021 gtacgtagtt aacatcggca aggctaattc caggcgcgct tgccagtaac cctgctgata
    25081 aacagagggc gatgagactt ttattcattt tcattgtttt cacctcaaaa tctggagctc
    25141 agcggtagcc aggcaatagc gcgctaaacc cgataatcag aggggctttc gcccctttca
    25201 gataatgaca acctgttttt atgccggatg cggcgtaaac gccttatccg gcccaggttt
    25261 tgctattacc accagatttc catctgggca ccgaaggtcc actcgtcgct gtcgccacga
    25321 ccgaagctgc cgccgttgaa atcagcagga acggctttgc cgtagttcgg gttaaccttg
    25381 ctatcgccgt tgtagtcgta accccatttc tcatcccact tggcgtaggt tgcgaagaca
    25441 cgaatagccg ggcgtgacca gatgctgtcg ccagcctgcc attgttgtgc gagggtaatt
    25501 ttgtactgat tgttcttgtc gccggtgcgc tgggattcga cgttgtcgta gccgatttcc
    25561 atcacggtgc tcatgattgg cgtccacttg tacatcgggc gaataccaac ggtccaccac
    25621 ttggtgccgt tgtcgttatc ccagttgata tcctggtaca tacccacgta catcatgtcc
    25681 cagttgtcgc ccatggagat cgcaccgtgg tcgaggatac gcagcatgtg accgttgttg
    25741 ttgatattgt aggcaaattt ttcgttatca aacgcgacgc cagaaccctg cgacagacct
    25801 ttaccctgcg aggtcatcga gtcagtagcg tactgaacaa caaacttgtt aaagcccttc
    25861 aggacactct gagtatgttc agcagtgaat aaccagccgt ctttcgatgc gccatcaacc
    25921 agacgatagt tatcacgcag gttggcacga ccgtagtcga cacccagttc taatgtgccg
    25981 cccgggttga tttccatctg cgctaaacgc acatcgaaaa cgtcgttcgc ggtttcgttg
    26041 gtatagtcat aaatattgtt gctggcaaaa gaggaagaac caccagcttc agaggagcgg
    26101 gttgctgcca gagagagttt accgaagcca acatcgatgt tttccagacc ggcaccagga
    26161 ccagaaatat cccagtagta gaagtcgatc atatgaacgt catgacgttg gtagaagcgc
    26221 ttacctgccc agatggtgga acctggcagc cattcgatca ggttttttcc ctgcacgttt
    26281 gcttcacgga aggccggatc ggtagcttcc cagtcattct gttgcgcgac ggaataggcc
    26341 acgttagtgt cgaaatagaa gctcttatcg ccctctttcc acacttcctg acccaatttt
    26401 aattcagcat aagtttcaca ttcgttgcca agacggtatt tactttgagc accggtagtc
    26461 tggaaacact gttgttcacc gccgctacct gtccagccaa taccggaacg tgcatagccg
    26521 tggaaatcaa cagccattgc ctgagcagac attacgcccg ctgcgacggc aaccgccaga
    26581 ggaagtttgc gcagagtaat catcattcta tctcctgagt cattgctttt cttttttcac
    26641 atcacctgtg acaggctttg tgtgttttgt ggggtgctta aacgcccggc tccttatgca
    26701 gtcgacgaca tgcagtgcca tcctcacgga acagatggca acgctctggc ggcaggccga
    26761 tagcgaatgt ggcaccttct tctaccaaca ccacgtcgtt ctggcggtac accaggtttt
    26821 gacgaatgga agggatctgg atatagattt gggtttcgtt gccgagttgc tcgacgacct
    26881 gaacttcacc ctcaaggatg acgtcagcga tatcactcgg cagtagatgt tccgggcgaa
    26941 tacccagcga catattggct ccaacctgga catcacggct ttcaacaggc agccagactt
    27001 gttgacgatt tggcatcggc agctccacct gcacttgatc gattgcggtg gcggtcactt
    27061 ttaccggcag gaagttcatc tttggcgaac cgataaatcc ggcgacaaaa cggtctgccg
    27121 gatagtggta cagttccagc ggtttcccaa cctgtgccac gcgaccggcg tccagcacca
    27181 cgattttgtc ggccagcgtc atcgcttcga cctgatcgtg ggtgacgtaa atcattgtgc
    27241 ggcccaggcg tttatgcaga cgggagattt cgatacgcat ttgcacacgc agtgcagcat
    27301 cgaggttgga gagcggttca tcgagcaaaa atacgcttgg ctcggccacc agcgtacggc
    27361 caatcgccac gcgctgacgc tgaccaccgg agagcgcttt cggtttgcga tccagcaaat
    27421 gcgccagttg cagcacttcc gccacctggt tgacgcgttg gttaatcacc tctttttttg
    27481 cgccagccag tttcaggcca aatgacatgt tttctgctac tgacaggtgg ggatagagcg
    27541 cgtaagactg aaacaccata ccaacgccgc gttctgctgg cggagtgtca ttcatccgtt
    27601 tctcaccgat gaacaggtcg ccgctggtga tcgtctcaag cccggcaatc atgcgcagta
    27661 aagtcgattt accgcagcca gacggtccga caaacaccac gaattcacct tcatggatat
    27721 cgagattgat atctttcgat accacgacct cgccccaggc tttcgttaca ttttgcagct
    27781 gtacgctcgc catgcccttc tccctttgta acaacctgtc atcgacagca acattcatga
    27841 tgggctgact atgcgtcatc aggagatggc ttaaatcctc caccccctgg cttttttatg
    27901 ggggaggagg cgggaggatg agaacgcggc ttctgtgaac taaaccgagg tcatgtaagg
    27961 aatttcgtga tgttgcttgc aaaaatcgtg gcgattttat gtgcgcatcc ccacattacc
    28021 gccaattctg taacagagat cacacaaagc aacggtgggg cgtaggggca aggaggatgg
    28081 aaagaggttg ccgtataaag aaactagagt ccgtttaggt gttttcacga gcacttcacc
    28141 aacaaggacc atagattatg aaaataaaaa caggtgcacg catcctcgca ttatccgcat
    28201 taacgacgat gatgttttcc gcctcggctc tcgccaaaat cgaagaaggt aaactggtaa
    28261 tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga
    28321 aagataccgg aattaaagtc accgttgagc atccggataa actggaagag aaattccctc
    28381 aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcgcacgac cgctttggtg
    28441 gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc
    28501 tgtatccgtt tacatgggat gccgtacgtt acaacggcaa gctgattgct tacccaatcg
    28561 ctgttgaagc gttatcgctg atttataaca aagacctgct gccgaacccg ccaaaaacct
    28621 gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt
    28681 tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt
    28741 tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga
    28801 aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg
    28861 attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc
    28921 cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga
    28981 ccttcaaggg tcaaccatct aaaccgttcg ttggcgtgct gagcaccggt attaacgccg
    29041 ccagtccgaa caaagagctg gcgaaagagt tcctcgaaaa ctatctgctg actgatgaag
    29101 gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg
    29161 aagagttggc gaaagatcca cgtattgccg ccaccatgga aaacgcccag aaaggtgaaa
    29221 tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtacgtact gcggtgatca
    29281 acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actcgtatca
    29341 ccaagtaatg ctgtgaaatg ccggatgcgg cgtgaacgcc ttgtccggcc tacaaaactg
    29401 aaacgcatgt aggcctgata agacgcatca gtgtcgcatc aggcagttgt tgtcggataa
    29461 ggcgtgaaag ccttatccgt cctggaatga ggaagaaccc catggatgtc attaaaaaga
    29521 aacattggtg gcaaagcgac gcgctgaaat ggtcagtgct aggtctgctc ggcctgctgg
    29581 tgggttacct tgttgtttta atgtacgcac aaggggaata cctgttcgcc attaccacgc
    29641 tgatattgag ttcagcgggg ctgtatattt tcgccaatcg taaagcctac gcctggcgct
    29701 atgtttaccc gggaatggct ggaatgggat tattcgtcct cttccctctg gtctgcacca
    29761 tcgccattgc cttcaccaac tacagcagca ctaaccagct gacttttgaa cgtgcgcagg
    29821 aagtgttgtt agatcgctcc tggcaagcag gcaaaaccta taactttggt ctttacccgg
    29881 cgggcgatga gtggcaactg gcgctcagcg acggcgaaac cggcaaaaat tacctctccg
    29941 acgcgtttaa atttggcggc gagcaaaaac tgcaactgaa agaaacgacc gcccagcccg
    30001 aaggcgaacg cgcgaatctg cgcgtgatta cccagaatcg tcaggcgctg agtgacatta
    30061 ccgccattct gccggatggc aacaaagtga tgatgagctc cctgcgccag ttttctggca
    30121 cgcagccgct ctacacactc gacggtgacg gcacgttgac gaataatcag agtggcgtga
    30181 aatatcgtcc gaataaccaa attggctttt accagtccat taccgccgac ggcaactggg
    30241 gggatgaaaa gctaagcccc ggttacaccg tgaccaccgg ctggaaaaac tttacccgcg
    30301 tctttaccga cgaaggcatt cagaaaccgt tcctcgccat tttcgtctgg accgtggtgt
    30361 tctcgctgat cactgtcttt ttaacggtgg cggtcggcat ggttctggca tgtctggtgc
    30421 agtgggaagc gttgcgcggc aaagcggtct atcgcgtcct gctgattctg ccctacgcgg
    30481 tgccatcgtt catttcaatc ttgattttca aaggattgtt taaccagagc ttcggtgaaa
    30541 tcaacatgat gttgagcgcg ctgtttggcg tgaagcccgc ctggttcagc gatccgacca
    30601 ccgctcgcac gatgctgatt atcgtcaata cctggctggg ttatccgtac atgatgatcc
    30661 tctgcatggg cttgctgaaa gcgattccgg acgatttgta tgaagcctca gcaatggatg
    30721 gcgcaggtcc gttccagaac ttctttaaga ttacgctgcc gctgctgatt aaaccgctga
    30781 cgccgctgat gatcgccagc ttcgccttta actttaacaa cttcgtgctg attcagctgt
    30841 taaccaacgg cggcccggat cgtcttggca cgaccacgcc agccggttat accgacctgc
    30901 ttgttaacta cacctaccgt atcgcttttg aaggcggcgg gggtcaggac ttcggtctgg
    30961 cggcagcaat tgccacgctg atcttcctgc tggtgggtgc gctggcgata gtgaacctga
    31021 aagccacgcg aatgaagttt gattaaggga gataacaaaa atggcaatgg tccaaccgaa
    31081 atcgcaaaaa gcacgtttgt ttattactca cctgctactg ctacttttta tcgcggcgat
    31141 tatgttcccg ctgctgatgg tcgtcgctat ctcgctgcgt cagggaaact tcgcgaccgg
    31201 tagcctgatc ccggagcaaa tctcctggga tcactggaaa ctggcgttag gttttagcgt
    31261 tgaacaggct gatggtcgca ttacgccacc gccattcccg gtactgctgt ggctgtggaa
    31321 ctcggtaaag gtcgctggga tttccgcgat tggcattgtg gcgctctcca ccacctgcgc
    31381 ctacgctttc gcccgtatgc gctttccagg caaagcgacg ctgctgaaag gaatgctgat
    31441 tttccagatg ttcccggcag tactttcact ggtcgcgttg tatgcgttgt ttgatcgtct
    31501 gggtgagtac attccattca ttggcctgaa tactcacggc ggcgtaattt tcgcgtatct
    31561 gggtgggatt gcgctgcatg tctggaccat caaaggctat ttcgaaacca tcgacagttc
    31621 gctggaagaa gcggctgcgc tggatggtgc gacaccgtgg caggccttcc gccttgtcct
    31681 gttgccgctg tcagtaccga ttctggcggt ggtattcatc ctgtcgttta tcgctgccat
    31741 tactgaagtt ccggtcgcgt cgctgttact gcgtgacgta aacagctaca ccctggccgt
    31801 ggggatgcag caatacctca acccgcaaaa ctacctgtgg ggtgactttg ccgccgctgc
    31861 cgtgatgtct gcattaccga tcaccatcgt cttcttgctg gctcaacgct ggctggtcaa
    31921 cggcctgacg gcaggtggtg tgaaaggtta aagatgttgt tctgccaatg ttatgccgct
    31981 gcaccctcaa cttacgttat cccaacttgt gactattatt cggcgctcca cggagcgcct
    32041 ttttttcttt cgtctgcaat ctgaatcgtt cgccggttaa tattcccatc atagaactta
    32101 ttatttttac gttatttgtt ttcccactta cgataattct ctttcgtgct ctgagtcacg
    32161 gcaatagtat tgttttcatc aatttttgat aattatcaca attaagatca cagaaaagac
    32221 attacgtaaa cgcattgtaa aaaatgataa ttgccttaac
//
LOCUS       DAJVPP010000042        31507 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVPP010000042 DAJVPP010000000
VERSION     DAJVPP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.042"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1360)
                     /gene="yeaG"
                     /locus_tag="OQM34_001896"
     CDS             complement(<1..1360)
                     /gene="yeaG"
                     /locus_tag="OQM34_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1452369.1"
                     /translation="MNIFDHYRQRYEAAKDEKFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYDRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHL
                     FYV"
     gene            1796..2542
                     /gene="mipA"
                     /locus_tag="OQM34_001897"
     CDS             1796..2542
                     /gene="mipA"
                     /locus_tag="OQM34_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1452370.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2632..3393
                     /locus_tag="OQM34_001898"
     CDS             2632..3393
                     /locus_tag="OQM34_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS1452371.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3444..4328)
                     /locus_tag="OQM34_001899"
     CDS             complement(3444..4328)
                     /locus_tag="OQM34_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1452372.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4412..5407)
                     /gene="gapA"
                     /locus_tag="OQM34_001900"
     CDS             complement(4412..5407)
                     /gene="gapA"
                     /locus_tag="OQM34_001900"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1452373.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5749..6162
                     /gene="msrB"
                     /locus_tag="OQM34_001901"
     CDS             5749..6162
                     /gene="msrB"
                     /locus_tag="OQM34_001901"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1452374.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6204..6476
                     /locus_tag="OQM34_001902"
     CDS             6204..6476
                     /locus_tag="OQM34_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1452375.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6846..7922
                     /locus_tag="OQM34_001903"
     CDS             6846..7922
                     /locus_tag="OQM34_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1452376.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7949..9328
                     /locus_tag="OQM34_001904"
     CDS             7949..9328
                     /locus_tag="OQM34_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1452377.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSTNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9345..10390
                     /locus_tag="OQM34_001905"
                     /pseudo
     CDS             9345..10390
                     /locus_tag="OQM34_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10411..11247
                     /locus_tag="OQM34_001906"
     CDS             10411..11247
                     /locus_tag="OQM34_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCS1452378.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11252..12199
                     /locus_tag="OQM34_001907"
     CDS             11252..12199
                     /locus_tag="OQM34_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCS1452379.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12209..13189
                     /gene="ydjG"
                     /locus_tag="OQM34_001908"
     CDS             12209..13189
                     /gene="ydjG"
                     /locus_tag="OQM34_001908"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCS1452380.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13326..14084
                     /locus_tag="OQM34_001909"
     CDS             13326..14084
                     /locus_tag="OQM34_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1452381.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14202..15560
                     /locus_tag="OQM34_001910"
     CDS             14202..15560
                     /locus_tag="OQM34_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1452382.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15653..16294)
                     /gene="pncA"
                     /locus_tag="OQM34_001911"
     CDS             complement(15653..16294)
                     /gene="pncA"
                     /locus_tag="OQM34_001911"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1452383.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16305..17321)
                     /gene="ansA"
                     /locus_tag="OQM34_001912"
     CDS             complement(16305..17321)
                     /gene="ansA"
                     /locus_tag="OQM34_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1452384.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17488..19344)
                     /gene="sppA"
                     /locus_tag="OQM34_001913"
     CDS             complement(17488..19344)
                     /gene="sppA"
                     /locus_tag="OQM34_001913"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1452385.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19505..20056
                     /locus_tag="OQM34_001914"
     CDS             19505..20056
                     /locus_tag="OQM34_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1452386.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20173..21216
                     /gene="selD"
                     /locus_tag="OQM34_001915"
     CDS             20173..21216
                     /gene="selD"
                     /locus_tag="OQM34_001915"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1452387.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21221..23182
                     /gene="topB"
                     /locus_tag="OQM34_001916"
     CDS             21221..23182
                     /gene="topB"
                     /locus_tag="OQM34_001916"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1452388.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23309..24349
                     /locus_tag="OQM34_001917"
     CDS             23309..24349
                     /locus_tag="OQM34_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1452389.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24466..25805)
                     /gene="gdhA"
                     /locus_tag="OQM34_001918"
                     /pseudo
     CDS             complement(24466..25805)
                     /gene="gdhA"
                     /locus_tag="OQM34_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
     gene            26041..26313
                     /locus_tag="OQM34_001919"
     CDS             26041..26313
                     /locus_tag="OQM34_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1452390.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26279..26686)
                     /gene="nudG"
                     /locus_tag="OQM34_001920"
     CDS             complement(26279..26686)
                     /gene="nudG"
                     /locus_tag="OQM34_001920"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS1452391.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26773..27393
                     /locus_tag="OQM34_001921"
     CDS             26773..27393
                     /locus_tag="OQM34_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1452392.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLTFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27402..28709)
                     /gene="ynjE"
                     /locus_tag="OQM34_001922"
                     /pseudo
     CDS             complement(27402..28709)
                     /gene="ynjE"
                     /locus_tag="OQM34_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28776..29429)
                     /locus_tag="OQM34_001923"
     CDS             complement(28776..29429)
                     /locus_tag="OQM34_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1452393.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29429..30946)
                     /locus_tag="OQM34_001924"
                     /pseudo
     CDS             complement(29429..30946)
                     /locus_tag="OQM34_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30919..31507)
                     /locus_tag="OQM34_001925"
                     /pseudo
     CDS             complement(30919..31507)
                     /locus_tag="OQM34_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7914 a         7532 c         8158 g         7903 t
ORIGIN      
        1 ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag ttgaacacgc
       61 gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca tcgacaccgg
      121 cgtagtcacg atcgtagtca cgatattcct gatacgactt ggctttcgga tcggtgtctt
      181 tcagactttc gccatcataa acccgcatct ttgaataaat gctggagttt tctggctctt
      241 tcaggcgcga aagaatggaa aaacgtgaca gtgtttcgag cgtgccaggg gcgcatgggg
      301 cgtgagtcaa ttcactgtga ttaagcaatt tctcgtagat tttgatctct tcggaaatgc
      361 gcaagcaata cggcaccttc acgatgtaaa cacggtcgag gaaggcttcg ttgtttttgt
      421 tattacggaa agtgacccat tcggacttgt tcgagtgggc gagaataatc ccgttgaacg
      481 gcagggcgga gataccttcc gtcccgttgt agttaccttc ctgagtggcg gttaacaagg
      541 gatgcagcac ttttatcggt gctttaaaca tctcaacgaa ttccatgatc ccctgattgg
      601 cgcggcacag cgcaccggaa tagccgtagg cgtccgggtc attctgcgcg tagtgttcga
      661 gtttacgaat atcgactttc ccaaccagcg cggagatgtc ctggttgttc tcatcaccgg
      721 gttccgtttt ggcgatagca atttgttgca gaattgacgg ccagaccttc actacccgga
      781 acttagtgat atcgccacca aattcatgca ggcgttttgc cgcccatggc gacatgatgg
      841 tgccgagata acggcgagga atgccatact ctttttccag aatctgagca tcttcctgcg
      901 gattgaaaag acagaacgga tgatcgttga ccgggctacg ctcaccgttg gcgctcaata
      961 cataaatcgg tacgagctgc attaatgatt tcagtcgctc agcaagcgat gatttacccc
     1021 cacccacagg ccccagcaga tacaggattt gtttcttctc ttccagcccc tgagccgcgt
     1081 gtttcagata agagacaatc tgttcaatcg cgtcttccat gccgtaaaac tcttcaaatg
     1141 ccggataacg tgcaatgacc cggttagaaa agagtcgaga aagtctgggg tcctgggctg
     1201 tatcgaccat gacaggctca ccgatagcca tcaatagccg ctcagccgcg ttggcataag
     1261 cactgcgatc ttgccgacaa gtggtaagaa actcctgcag tgtgaacttt tcgtccttgg
     1321 cagcttcata tcgctggcga tagtgatcga atatattcat ggtatgccgt cctttcgttt
     1381 tttagcacag gttaagagcc gttcatatga atagtagagg ctcccggaag aggtaaactg
     1441 aacgacgtct cttgctaaaa cacagcaact ctcatgccat tttatcgacg atgagaacaa
     1501 cgtgcccggt gatacacctt caaaattaag cgtagatggc aaatggaaaa cttgcctgca
     1561 cggctttctt aatttcaatg gcatatcaac aactcatcca taaattttgc ataattaatg
     1621 taaagaccag gctcgccagt aacgctaaat ccatttggct gtaagcgcgg tgtcatccgc
     1681 ggcaggaaaa ttaaacagtt actttaaaaa atgaaaacgt aaaaaggttg ggtttcgatg
     1741 tattgacggg taaactttgt cgcccgctaa acatttgttt aaggaatgat taattgtgac
     1801 caaactcaaa cttctggcac ttggagtgct tatcgcaacg tctgcaggcg tagcgcacgc
     1861 tgaaggtaaa ttttccctgg gcgcaggcgt aggtgtcgtt gagcacccat ataaagatta
     1921 cgataccgat gtttacccag taccggtaat caactatgaa ggcgataact tctggttccg
     1981 tggcttaggt ggtggttact acctgtggaa tgacgcaacg gataaacttt caattaccgc
     2041 ttactggtcg ccgctttact tcaaagcgaa agacagtggc gatcaccaaa tgcgtcacct
     2101 ggatgaccgt aagagcacca tgatggctgg tctgtcttat gctcacttta cccagtacgg
     2161 ttacctgcgt accaccctgg ctggcgatac cctggataac agcaacggca ttgtctggga
     2221 tatggcctgg ttgtatcgtt acaccaacgg tggcctgacc gtgactccgg gtattggtgt
     2281 gcagtggaac agcgaaaacc agaacgaata ctattatggc gtatcgcgca aagagtccgc
     2341 tcgcagcggt ctgcgtggct ataacccgaa tgacagctgg agcccttacc ttgagctgag
     2401 cgccagctac aacttcctcg gcgactggag tgtttacggt accgcgcgct acacccgtct
     2461 gtctgatgaa gttactgaca gcccgatggt ggataaatcc tggactggcc tgatttctac
     2521 cgggatcacc tacaaattct gataatgcat ctttccaggg cattttgtgc ataaaaacag
     2581 ggcgtcacgc cctgttttgc attacggtgg tgcaatacct gggaggaaac aatgcaacaa
     2641 aaaatgattc aatttagtgg cgatgtctca ctgccagcca tagggcaggg aacatggtat
     2701 atgggcgaag atgccagtca gcgcaaaaca gaagttgctg cactacgcgc aggcattgaa
     2761 ctcggtttaa ccctcattga taccgccgaa atgtatgccg atggcggtgc cgaaaaggtg
     2821 gttggggaag cattaaccgg tctgcgagag aaggtctttc tcgtctctaa agtctatccg
     2881 tggaatgctg gcgggcaaaa agcgataaat gcatgcgaag ccagtttacg ccgtctcaat
     2941 actgactatc tcgatctcta cttattacac tggtctggca gtttcgcttt tgaagagact
     3001 gttgctgcga tggaaaaatt gatcgcccag ggaaaaatcc gtcgctgggg cgtttctaac
     3061 cttggttatg ccgatatgca ggaactctgg cagctgccgg ggggaaatca gtgtgccact
     3121 aatcaggtgc tttaccatct cggttcacga gggattgagt acgatctact cccctggtgc
     3181 cagcaacagc agatgccggt gatggcttac agtccattag cccaggccgg gcggttgcgc
     3241 aatggactgt taaaaaacgc ggtagtcaac gaaattgcac atgtccaaca acatgcagct
     3301 gcgcttgagg tcgaactttc gtcagcggaa ttagctatgc tggataaggc atatccggca
     3361 ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat acgtgccgga tgcatcgcac
     3421 ccggcacaca acattaacgc aaattaacgt ttggcaacgc gaatggattg cgccagatgt
     3481 gcaggtttct ctttggtgac tttttgcgtt tctgaagcgt aagccgtttc tacacaaaca
     3541 aaggtcttgt agccatcgtc cggcatgtcg cccatgctaa ctgaaagcgc cggtcccggg
     3601 ttccagccga caacgttcag atgatgctgg tggcctacgg cgataatacg attcagcgct
     3661 tcatcattaa tcacgctgca atcttgcgga ttcagataca cgcgatcggt acggtcaggg
     3721 aaggtctgaa taccgtcggt cagtacatct tctttcgcgt cattcacttt atcaatgaag
     3781 cgatcgccca gcccactgac gcttacctta gcgatatcac ccacgttaaa gtaggtatgc
     3841 agggcagagg tggtttcaaa ttcgccatgt gattcaagat cgatttcaca ggttttaccc
     3901 acgcggaaat gcgctaacag cgtaaagtcg tgcggccaga attttttcgt ctcttcgctt
     3961 tgtgtcaatt caaaagtcag cgctacgcca tcagcatctt catgatgtga tttcagcgtc
     4021 cacggcaggt tgcgggcaaa accgtgcgca ggcagacctt gttgtgccgc cggaccaaac
     4081 cacggccagc aaaccggtac gccaccgcga atagcgacgc catttttgaa cggtgtgttg
     4141 ttgctcaacc acagaacttc ttcttcaccc gcaggtttcc acgagagaag gtgtgcgccc
     4201 tgtaatgcaa aagaggcttt tacctgggga tgatcgacca caatgaggtc cagttcatcc
     4261 agtttacgac gggagaggac aggggagatt tgttcgatga ccggaagggc aaaaattttc
     4321 ttaatcatga cgcagtcctt taacttcatt ttatcaggta aaaaaaagag cgaccgaagt
     4381 cgctcttttt agatcacagt gtcatctcaa cttatttgga gatgtgagcg atcaggtcca
     4441 gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga taccagtttc acgaagttgt
     4501 cgttcagagc gataccagct ttagcatcga acacggaagt gcaaacttcg ccgttgaaat
     4561 cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag
     4621 cagctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga cgaacggtca
     4681 ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc cataccagtc agtttgccat
     4741 tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaggac gggatgatgt
     4801 tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca acggttttct
     4861 gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg aagttatcgt
     4921 tgataacttt agccagcgga gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt
     4981 cctggccagc atatttgtcg aagttagcgc ctttaacgaa catcggagtg ttgtctttag
     5041 acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt
     5101 cagtcaggaa cagaccagtt gcttcagcga caacgtcaac acccacttcg tcccatttca
     5161 ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg atcagatgac
     5221 cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt
     5281 atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt
     5341 tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata cctactttga
     5401 tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta aaattacaaa
     5461 aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct
     5521 aagcattacg cgactgactc gcctcacttt tcctttgggc ttgagaccac atggggtcgg
     5581 cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg atttaggtgc
     5641 agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt aatgttttgt
     5701 tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagtaaat ggcgaataaa
     5761 ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttttacgt gacgcagaat
     5821 catgggacag aaccgccatt tacgggtcgt ttactgcata acaagcgtga cggcgtatat
     5881 cactgtttga tctgcgatgc cccgctgttt cattccgaaa ccaagtatga ttccggctgt
     5941 ggctggccca gtttctacga accggtgagt gaagaatcca ttcgttatat caaagacttg
     6001 tcacatggaa tgcagcgcat agaaattcgt tgcggtaact gtgatgccca tctggggcat
     6061 gtctttcccg acgggccgca accaacaggc gaacgttatt gtgttaactc tgcttcttta
     6121 cgcttcactg atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact
     6181 tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc atgacgcctg
     6241 aggtatacca gcgtttgtcg accgccgttg aactggggaa atggcctgat ggcgttgcgt
     6301 tgaccgagga acaaaaagag aattgcctgc aactggtgat gctgtggcaa gcccgccata
     6361 ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac
     6421 agcagttaaa agaagatttt ggtatctcag caaagcctat tgccatgttt aagtaagtgg
     6481 ttcatcccct ttttgccgcg gctatgaagg ggatagccaa caatgacccc acgactcgtc
     6541 tcaattatct cgacccgacc tggctaagtc ccagtccgat ctcttgcagt cgcgttcacg
     6601 cctgaatgac ttctcctgaa cttaagtctc agacctattt ggccggtaat ccctctcgaa
     6661 tttgctcttt ttgcgtcccg ccttccacca acctgtaaca gacccagcca ttttctgcgt
     6721 aattgtgatc tgagttgtaa aatgcaggtc attatctgtg ttgtgcagat ttaatattga
     6781 cctcaaaaat catgtgggta agtatgatgg ccatctaagc aaaacaaaac acagaggatg
     6841 acgcaatgaa agcactggct cggtttggca aggcctttgg cggctacaag atgattcatg
     6901 tcccacaacc catttgtggc ccggaagacg tagtgattga aattaaagcc gcggcaatct
     6961 gcggcgcaga catgaagcac tacaatgtcg atagcggttc tgatgagttt aactctatcc
     7021 gcggccatga gttcgcaggt tgtattgcgc aggttggtga aaaagtcaaa gactggaaag
     7081 tggggcaacg cgtcgtgtcg gataacagcg gtcacgtttg cggcgtttgt ccggcctgtg
     7141 aacagggtga ttttctgtgt tgtacagaaa aagtaaacct tggtctggat aacaatacct
     7201 ggggcggtgg tttttccaaa tattgtctgg ttcctggtga aattctcaaa attcatcgtc
     7261 atgcgttgtg ggaaatccct gatggtgttg attatgagga cgcagccgta cttgacccta
     7321 tctgtaatgc ctataaatcc atcgcgcagc aatcgaaatt ccttcctggt caggatgttg
     7381 tcgtcattgg cactggccca ctcgggctgt tttccgtaca aatggcgcgg attatggggg
     7441 cggtaaatat cgtcgtcgtt ggtctgcaag aagatgtggc ggtccgcttc ccggttgcaa
     7501 aagaactggg ggcgacggca gtagtaaatg gttctaccga agatgtggtg gcgcgctgcc
     7561 agcaaatttg tggcaaagat aatctgggac tggtgattga atgctccggt gccaatatcg
     7621 cactgaaaca agccatcgaa atgctccgcc cgaatgggga agtggtacgc gttggaatgg
     7681 gcttcaaacc tcttgatttc tcgattaatg acattaccgc ctggaataaa agcatcattg
     7741 ggcatatggc ctatgactcc acctcatggc gtaacgctat ccggctatta gccagcggcg
     7801 ctatcaaagt caaaccgatg atcacgcatc gtatcggcct gtcgcaatgg cgcgaagggt
     7861 ttgatgcgat ggtcgataaa accgcaatca aagtgatcat gacttacgac tttgatgaat
     7921 aactactctc ctgaaggaac gttatcgcat ggaacagata acaaaaccgc attgtggtgc
     7981 acggctggat cgcttaccgg attgccgctg gcattcgtca atgtttgcca tcgtcgcgtt
     8041 tggcttgctt gtctgctgga gtaatgccgt tggtggcttg atcctcgcgc agctgaaagc
     8101 gttgggctgg acagataatt ccactacagc cacattctca gcaatcacga ccgccggaat
     8161 gtttctcggt gcgctggtgg gcggcatcat tggcgacaaa accggtcgca gaaatgcgtt
     8221 catcctctat gaggccattc atattgcctc gatggttgtc ggtgctttct caacgaatat
     8281 ggattttctt attgcctgcc gttttgtgat ggacgttggg ctgggagctt tactggttac
     8341 actgtttgct ggtttcaccg aatatatgcc cggtagaaat cgcggaacgt ggtccagtcg
     8401 ggtttccttt attggcaact ggtcatatcc gctctgctca ctgattgcga tgggactcac
     8461 gccgctgatt agtgcagagt ggaactggcg ggtacaactg cttattcctg caatattgtc
     8521 gcttatcgct acggcgctgg cctggcgcta ctttcctgaa tccccgcgct ggctggaatc
     8581 gcgcggacgg tatcaggaag ccgagaaagt catgcggagt atagaagaag gcgtcatacg
     8641 ccagacgggt aaacctttgc cgcctgtggt tattgctgat gacggtaaag cgccacaagc
     8701 ggtgccgtat tcagccttac tgacaggagt attactgaaa cgcgtgatat taggctcttg
     8761 tgtgctgatt accatgaacg ttgtgcagta cacactaatt aactggttgc caacaatatt
     8821 catgacccag gggattaatt taaaagactc gattgtttta aataccatga gtatgtttgg
     8881 cgcgccattt ggtattttta ttgccatgct ggtgatggat aaaattccgc gtaaaacaat
     8941 gggtgtgggg ctattaatcc tgattgcggt gctcggatat atctattcac tgcaaaccag
     9001 tatgttgctc ataacgctga ttggtttctt cctgattact ttcgtctata tgtacgtttg
     9061 ctatgcctcg gcagtgtatg tccctgaaat ctggccgaca gaggccaaac tccgtggctc
     9121 cggtctggcg aatgcggtag ggcgaatcag tggtattgcc gcaccttatg ccgttgcagt
     9181 gctgctcagt agttatggtg taacgggagt ctttattctt ctgggggctg tttcaaatat
     9241 tgtcgcaatt gctatcgcca ccattggaat tgaaaccaaa ggtgtctccg ttgaaagttt
     9301 aagtattgat gcagtagcca ataaataata cgggtggatt caaaatgaaa aattcaaaag
     9361 caatattgca ggtgccgggc acaatgaaaa ttatttcagc agaaatacca gtgcctaaag
     9421 aagatgaagt tttgattaaa gtagaatatg tcggtatttg tggttcagat gtacatggtt
     9481 ttgaatcagg cccgtttatt ccgcctaaag acccaaatca agaaattggc ctgggtcatg
     9541 aatgcgccgg gacggttgtg gctgtgggaa gccgcgtgcg caaatttaaa ccgggggatc
     9601 gggtaaatat cgaacctggc gttccttgcg gtcactgtcg ttactgtctg gaaggcaaat
     9661 ataacatctg cccggacgtt gattttatgg cgacacaacc caactaccgc ggcgcattaa
     9721 cgcactatct gtgtcatccg gagagcttta cttacaaact gcccgacaat atggacacga
     9781 tggaaggggc gctggtggag cctgccgcag tcgggatgca tgccgcgatg ctggcagatg
     9841 ttaaaccggg taagaagata attattctgg gagcaggttg tattggtttg atgacgttgc
     9901 aagcgtgcaa atgcctgggc gcaacggaaa ttgccgtcgt tgatgtactg gaaaaacgtc
     9961 tggcaatggg ggaacagcct ggtgcgacag tggttattaa tggcgcaaaa gaggacacta
    10021 ttgcacgctg tcagcaattt accgaagaca tgggcgcaga tattgttttc gaaacagcgg
    10081 gttctgcggt caccgttaaa caggcacctt taatctggta atgcgcggcg gtaaaattat
    10141 gattgttggt actgtacccg gcgattcggc aatcaatttc ctcaaaatca atcgcgaagt
    10201 cactatccag acggtattcc gctatgccaa tcgttatccg gtcacgattg aagctatttc
    10261 ttcagggcga ttcgatgtga aatcgatggt gacgcatatt tacgattatc gggatgtaca
    10321 acaggcattt gaagagtcag ttaacaacaa acgcgacatt attaaaggcg ttattaaaat
    10381 tagcgattaa ttaatacggg agaacagaac atgctcgcag atatcagata ttgggaaaac
    10441 gatgccacaa ataaatatta tgcaattgcc cattttaacg tatggaatgc agaaatgttg
    10501 atgggcgtta aagacgcagc cgaagaagcg aaatccccgg ttattatttc ttttagtaca
    10561 ggttttgtcg gtaacacctc atttgaagat ttctctcaca tgatggtatc aatggcacaa
    10621 aaagcaacgg tgccggtaat aactcattgg gatcatggtc ggagtattga gattattcat
    10681 aacgcctgga ctcatggtat gaattcatta atgcgtgatg cttctgcatt tgatttcgaa
    10741 gaaaatattc gtttaaccaa agaggctgtt gatttctttc atccgctggg tattccggta
    10801 gaggcggaat tagggcatgt cggtaatgaa accgtttatg aggaagcgct ggcgggctat
    10861 cactataccg atcctgacca ggcggctgaa tttgttgaac gtacgggctg tgactcattg
    10921 gctgtcgcca tcggcaacca gcatggggtt tatacgtcag agccgcaatt gaactttgag
    10981 gtcgtcaaac gcgtacgcga tgctgtttct gttccgctgg ttttgcatgg cgcatcgggg
    11041 atcagtgatg ccgacattaa aactgcaatt tcgttgggta ttgcgaaaat caacattcat
    11101 acggagctct gtcaggccgc aatggtagca gtgaaagaaa accaggatca gcctttcctg
    11161 catttagaac gtgaagtacg taaagctgta aaagaacggg cattggaaaa aattgaactg
    11221 ttcggttctg atggcaaagc ggaataatga tatggataat ctcgacgtta tttgtatagg
    11281 tgccgctatt gttgatattc cattacaacc ggtcagtaaa aatatctttg atgtagattc
    11341 ttatcctctt gaaagaatcg caatgagcac cgggggcgat gcaataaatg aagcaacaat
    11401 tatttctcgt ctggggcatc gtaccgcatt aatgagccgc attggtaaag atgcctcagg
    11461 gcaatttatt ctcgaccatt gtcgcaaaga gaatattgat attcaaagcc tgaaacagga
    11521 cgtcagcata gatacatcta ttaacgttgg actggtgacg gaggatggcg agcggacgtt
    11581 tgtcacgaac cgtaacggca gtttgtggaa attaaatatt gacgatgttg attttgcgcg
    11641 gtttcctcag gcgaaattat tatcactggc cagtattttc aacagtcctc tattggatgg
    11701 taaagcgcta acagaaattt ttacgcaagc gaaagcccgg cagatgatca tttgtgccga
    11761 tatgatcaaa ccgcggttga atgaaacgct ggatgatatt tgcgaagcat tgagttatgt
    11821 cgattatctg tttccaaatt ttgccgagga aaaattactc acagggaaag agacactgga
    11881 tgaaattgct gactgctttc ttgcgtgcgg cgtaaaaacg gtggtgatta aaacgggtaa
    11941 agacggctgc tttatcaagc gtggtgacat gacgatgaag gtgccggcgg tcgcaggaat
    12001 aaccgccatc gacaccattg gcgcgggcga taactttgct tcaggtttta ttgcggcact
    12061 gttagaaggc aaaaatctgc gtgaatgcgc acgctttgcc aatgcaacgg cggctatctc
    12121 ggttctaagc gtcggtgcca ccaccggcgt aaaaaacaga aagctggtcg aacaattgct
    12181 ggaagaatac gaaggataat gaaggcaaat gaaaaagata cctttaggca cgacggatat
    12241 aacgctttcg cgaatggggt tggggacatg ggccattggc ggcggtcctg catggaatgg
    12301 cgatctcgat cggcaaatat gtattgatac gattcttgaa gcccatcgtt gtggcattaa
    12361 tctgattgat actgcgctag gatataactt tggcaatagt gaagttatcg tcggtcaggc
    12421 gttaaaaaaa ctgccccgtg aacaggttgt agtagaaacc aaatgcggca ttgtctggga
    12481 acgaaaagga agtttattca acaaagttgg cgatcggcag ttgtataaaa acctttcccc
    12541 ggaatctatc cgcgaagagg tagaagccag cttgcaacgt ctgggtattg attacatcga
    12601 tatctatatg acgcactggc agtcggtgcc gccatttttt acgccgatag ctgaaactgt
    12661 cgcagtgctt aatgagttaa aggctgaagg gaaaattcgc gctataggcg ctgctaacgt
    12721 cgatgctgac catatccgcg agtatctgca atatggtgaa ctggatatta ttcaggcgaa
    12781 atacagtatt ctcgaccggg caatggaaaa cgaactgctg ccgctatgtc gtgataatgg
    12841 cattgtggtt caggtttatt ccccgctaga gcagggattg ttgaccggca ccatcactcg
    12901 tgattacgtt ccgggcggcg ctcgggcaaa taaagtctgg ttccagcgtg aaaacatgct
    12961 gaaagtgatt gatatgcttg aacagtggca gccactttgt gctcgttatc agtgcacaat
    13021 tcccactctg gcactggcgt ggatattaaa acagagtgat ttaatctcca ttcttagtgg
    13081 ggctactgca ccggaacagg tacgcgaaaa tgtcgcggca ctgaatatca acttatcgga
    13141 tgcagacgca acattgatga gggaaatggc agaggccctg gagcgttaaa tactcaacac
    13201 gattcacctc tgtttctgtg aaaactgtga tgaaaatctg tgattcaaat aggttatttt
    13261 gaaagtaaac atcggttatg cgataatcgc gctaatgtga tgtaaaaaga tacaggggtg
    13321 taatcgtggc ggcaaaagac aggattcagg caattaagca aatggttgcc aacgataaaa
    13381 aggtgacagt ctcaaatttg agtgggattt ttcaggtaac cgaagaaacc attcgccgcg
    13441 atcttgagaa gctggaagat gaaggctttt tgaccagaac ctatggtggt gctgttttaa
    13501 atacagcggt gttgacggag aatatccatt tttataagcg cgcttcatcg ttttatgaag
    13561 agaagctgct tattgcacgc aaggcactac cctttatcga caataaaacc accatggcag
    13621 ccgattccag tagtaccgta atggaattgc tcaaattgtt acaggaccgt agtgacctga
    13681 cgttgctaac caactcggcg gaagcgattc atgttctggc tcagtcagaa attaaagtcg
    13741 tttcaacggg tggggaacta aacaaaaata cactttccct gcaaggaaga attactaaag
    13801 agatcatcag ccgctaccat gtcgatatca tggtaatgag ttgtaaaggt cttgatatta
    13861 acagcggcgc gctggactct aatgaagcgg aagctgaaat caaaaagaca atgatccgtc
    13921 aggcgacaga agttgcgtta ttggttgatc actctaagtt tgatcgtaaa gcttttgtcc
    13981 agttagctga ctttagtcat attaattata taataactga taaatcaccg ggtgcagagt
    14041 ggatagcatt ttgcaaagac aataatatcc agctggtgtg gtaatttaag cacacgttgt
    14101 caatattatt tgttcctgcg actggaaata aaatgatgat aaagatgcgg caagtgaatt
    14161 tgccgcaatt gctacctttt atagcaaatc cgaggcaccc aatggaacaa tatgatcaaa
    14221 ttggcgcaag actggaccgc ttgcctttgg cccggtttca ttatcgtata tttggtataa
    14281 taagctttag tctgttatta acggggtttt tgagttactc aggtaatgtc gtcttagcaa
    14341 agctggtaag caatggatgg tcaaataatt tcctctatgc cgcctttacc tcggcattaa
    14401 tgtttggtta tttcatcggc tcacttactg gtgggtttat tggtgactac tttgggcggc
    14461 gcagggcgtt tcgcataaat cttctcatcg tcggtattgc tgcaacaggg gccgcttttg
    14521 tccctgatat gtactggctc attttctttc gcttcctgat gggaacagga atgggggcgc
    14581 tgattatggt tggctatgcc tcatttacgg agtttatccc cgcgacggtg cgtggaaaat
    14641 ggtccgcgcg gctctcattt gttggtaact ggtcgcccat gctgtctgcg gcgataggcg
    14701 tggtggttat cgcttttttt agttggcgaa taatgtttct tctgggtggt attggcatac
    14761 tgttagcctg gtttctctca ggtaaatact ttatcgagtc gccacgatgg ctggaaggga
    14821 aagggcaaat cgccggtgca gaaagccaac ttcgtgaagt agagcagcaa attgaaaggg
    14881 agaagagtat tcgtttaccc cagcttactt tgaaccagag caacagcaag gttaaggtaa
    14941 tcaagggtac cttcaggctc ctgtttaaag gggaaatgtt acgacgtaca ttagttgcga
    15001 taactgtttt aattgcaatg aacatttcgc tttataccat taccgtatgg ataccgacca
    15061 tatttgttaa ctccggcatt gatgtcgata aatcaatatt aatgaccgct gttattatga
    15121 ttggtgctcc ggtaggaata tttattgcgg cattaattat tgatcatttt cctcgtcgat
    15181 tatttggctc cgccttactt attattattg ccgtgttagg ctatatctat tcaagtcaga
    15241 ctacagagtg ggcgatttta atctatggtc tggtgatgat cttcttttta tacatgtatg
    15301 tttgcttcgc gtcggcggtt tatatcccgg agctttggcc aacgcattta cgcctgcgcg
    15361 gttcgggttt cgttaatgcc gtcggacgga tcgtcgcagt tttcacgccc tatggcgttg
    15421 cggcattatt aacacattat gggtcgatca cggtgtttat ggtgcttggt gtcatgttag
    15481 tgctctgtgc gctggttctc tccatttttg gcatcgaaac gcggaaggtg tcgctggaag
    15541 agatttctga ggtgaattaa atctttcgtt gatggcatca attgccagat gtaccactga
    15601 cgtgttttat ttggcctaca atgccagtac agaattgtag gccagcgtaa aattacccct
    15661 gcgtctcttc ccagtctgcc agcgtatata gcgttgcccc agctgctgac atctccataa
    15721 acgcgtgtgc actgtcctgg ggctggatat tcacgccacg acagccatcg gtaatcacgt
    15781 ttaccttata acctaactgt aacgcgtcca gcacggtaaa cttcacgcaa tagtcagtag
    15841 ccaggcccat aacgatcaat tcatcgattt catgatcgcg caaccagtca tcaagcgcgg
    15901 ttttctgccg acgtccgtta tcaaaaaagg cactgtaact gtcaactaaa ggattttcgc
    15961 ctttatggaa caccgctgcg atctcttttt gattcagtaa cggatgtaat tgtgcgcctt
    16021 cactgttctg cacacagtgt tcaggccaga aagtttgccg caggccgccg agttggcctg
    16081 gcgtataagg ctctacgccg tgctggctgg caaagctgcc gtgattcgcc ggatgccagt
    16141 cctggctggc gataaccgct tctccgcgcg actggcacca gtcaataagg cggttagcaa
    16201 cctccaccgt actgtcacct tccggtacgg cgagcgcgcc accagcacag aaatcatttt
    16261 gtaaatcgac cagtaacagg gcgcgatggg gcattgcagt ctccttaatc atccggcgtc
    16321 agttcgccgc gcaggttttg gctcatggcc ttgcgaatgg tttcagtatc cagttcctgg
    16381 ctcagcaggt aatgcagttt ggttagcgtg gcttctacag tcatatctgc accgtcaata
    16441 acgccggcat gggcgagggc gttaccggtg gcataaccac tcatattcac tttaccggac
    16501 atacattgtg tcaggttgac caccacaata ccgcgatcgc tggcttcttg taattcctgc
    16561 aggaaggctt tgttttgtgg cgcattaccc acgccatagg agcgcagaat caatgctttc
    16621 accggttggc gcagaaaatt gcgcacgacg tcagcagaaa tccctggata aatcgtcacc
    16681 acgccaattg gttgtggcgt gattggatga acgattaatt ccccttcacc gtgcggggcg
    16741 ggtggcgtat tcaaacgacg aatatggata ccagcttcca gtaacggagg aaggtttgga
    16801 gaggcaaacg catcaaaacc atcggcatgg gctttggtag tgcggttgcc gcgatacaat
    16861 cggttattga aaaagagcgt tacttcgttg atcggataat tcgccgcaac gtacaacgca
    16921 ttcagcagat taatttgtcc gtcagagcgt aactcagcca gcgggatttg tgaccctgtc
    16981 acaatgaccg gtttaccgag attctcgagc atgaacgaca gcgcagaggc ggtatacgcc
    17041 atcgtgtcgg taccgtgtag aatgacaaaa ccatcatagt cgtcatagtg cgctttaata
    17101 tcttcagcaa tatgctgcca gtcttccggc gtcatatcag aagaatccat caacggcgta
    17161 tattcatgaa tggtgaaatc tggcatctcc gggcgatgga attccggcat cagcgccagt
    17221 tggcgttgta gatgacctga caccggaata taaccctgct cggaacgctg catcccgatg
    17281 gtcccgcccg tgtaggcgac gtaaattgat ttcttttgca tgatattgat atgaatgata
    17341 tcgaaagagc aaaagtatat acgtgaggca ggaggagcgc acctgatact atgcggataa
    17401 ggaaatatgg ggcgagacag cgggaagagg attcgtcgag gtaaaaaata atgtggccga
    17461 cgatctgtcg gccactcagt acaagactta acgcacgttg gcgcaggtca ggcaaaacgc
    17521 ataacggttt tgtgggtcgt taaacgcggc cagcttgtcg ctttcacttt taacggtaga
    17581 ggctaccgag gcaagcggtg caggtaacat ggcctggaac gcatctggca acattgcccg
    17641 gacagaacca gacatgttgt ccatcacttt gtcgaagaag gtcggttcat caacgtagta
    17701 ttccagatgc cactgtttca cttttgcgag ctctgctgct ttggcaaccg catcatcgaa
    17761 atccccgaga ctatcgacca gcccgttagc ttttgcatcc tgaccggtcc agacgtggcc
    17821 ctgggcgatt ttatcaattt gctccggcgt cgaatgacgc gcatcagcaa ccagcgtgat
    17881 aaagcgttta tagccattct caatgcttaa ttgcatcatc tgctgcgctt ccggcggcag
    17941 tgccctggtg atagaaacat ccgccagcgg tgaagttgag acaccatcag tatgaacacc
    18001 aatcgaatcc agactatttt ctacggtggt gatcacgccg aagataccga tagaaccggt
    18061 cagggtgctg gggttagcca caatgtaatt agctggcgtg gaaatccagt aaccaccaga
    18121 tgccgccatg ccgcccatcg atacaaccac agacttaccc gctgcccggg ctgctgccag
    18181 ttcagcgcga atcacttcag acgcggtaac gctgccgcct gggctattaa cacgcaggac
    18241 aatcgctttc actttcgggt caaggcgagc atcgcggatt tgtgccgcag tggtatcacc
    18301 gccaacattc ccctgagttt cctcgccatc cataattgcg ccattagcga agacgacacc
    18361 gatgctgtca ccggtatctg ccggcgtttt caatgcgtaa tcgtaataac tgatggcgcg
    18421 ataattttta tcagtcttac tccagccgaa ctctttggtc agagcttttt cgatttcggc
    18481 actcgatgcc agtgcatcga ccagcttgtt ttccagtgca tatttcgcgg tatcgccacc
    18541 ggttttggtt aaaccctcaa gcaacccttg cgcgccaggg aatacctgct gagcagggat
    18601 ctgccggtta gcggcaacag tattcagata gttttgccac agctcaccaa tccagcggct
    18661 gtcagcttcg cgggctgccg gtgacatatc atcacgaata aacggttcaa cggcagattt
    18721 atacgtaccc acgcggaaca catgggtgga aactttcagc ttatccagca acgatttgta
    18781 gtacagaccg ttggtagcaa aaccgtgcag atcaaccacg ccttgcggtg acagccaaat
    18841 tttattggcg aaactggcga gataatattg cccctggctg tagttctcgc caacggcata
    18901 aaccggtttc ccgctgtcac gaaactcttt cagggctttg ccgatgtact gcatagacgg
    18961 ctggtcgccg cctgcgaagt ttttcagatc catcacgata ccggtgatat tgcggtcgtc
    19021 cttcgcctgg cgaatggtgt tgacgatatc aaacagtgag ttttcctgca gacgatcgga
    19081 actggcacca agcagctggc ggcttaattt actaaaccgc tgagaactgt ctggtttatc
    19141 gacgatcaca ccagaaatgt ccagcagcag tgcgccacga ctggccgttt ctttcgaatc
    19201 accaccactg acctgcatcc agatccccac accaaccagt acgaggaaaa taaagaacag
    19261 gttaagtacc atttcacgga cgaaattcag cagacgccac gtccatttaa aaaatccggc
    19321 aataaatcgc caaagggttc gcatgtattc tcccaactta aggtcacacc tgtcacaggc
    19381 gcaatagatg tggttatcgt aatgacccaa ccggcacttg tcagcaggaa ttgcggactg
    19441 tgctgtaaca aaatccactc tcgtgttaat tttgtgaata aatatcacga caggagttaa
    19501 tcaaatggat gcactcgaac tattgatcaa tcgccgtagc gcctcccgcc tggctgaacc
    19561 cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg ggtatgcgtg cgccggacca
    19621 taagtccatg caaccgtggc atttttttgt gattgaaggg gaagggtgcg agcgtttcag
    19681 cgccgtactg gaacaggggg cgattgctgc cggtagtgat gataaagcta tcgacaaagc
    19741 ccgtaatgcg ccgttccgcg caccgctcat catcacggtg gtggcgaaat gcaaagaaaa
    19801 tcataaagtc ccgcgatggg aacaggaaat gtctgccgga tgcgcggtca tggcgatgca
    19861 aatggcagcc gttgcccagg gatttggcgg catctggcgc agtggcgcat taactgaaag
    19921 tccggtagtg cgtgaagcat tcggttgccg tgagcaggat aaaattgtcg gttttctcta
    19981 cctcggcacg ccgcagttga aagcatctac gtcgattaac gtcccggacc cgacgccgtt
    20041 tgtaacttat ttctgataat tgtcgcgaaa ctgtctggat tgtgagcaaa ggcgcaggaa
    20101 ttcagacact ctcacttatc acttcacgga atgagggtta ccatacccgg attgcattga
    20161 caggagatgt ccatgagcga gaactcgatt cgtttgaccc aatacagcca cggagctggt
    20221 tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc tgcacagtga gcaggcgaag
    20281 tttgttgatc cgaatttgct tgtgggtaat gaaacccgcg acgatgcggc ggtgtacgat
    20341 ctgggcaatg gcaccagcgt tatcagtacc accgacttct ttatgccgat cgttgataat
    20401 cctttcgatt ttggccgcat tgcggcgact aacgccatca gcgatatctt cgcgatgggg
    20461 ggcaaaccga ttatggcgat tgcgatcctc ggctggccga ttaacaaact ttccccggaa
    20521 attgcccgcg aagtaaccga aggtggacgc tatgcatgcc gtcaggcggg tattgcgctg
    20581 gctggcggtc actccatcga tgcgccggag ccgatttttg gtctggcggt aacggggatc
    20641 gtaccgaccg agcgggtgaa gaaaaacagt accgcacaag ccggatgcaa actgttcctg
    20701 acgaaaccgc tggggatcgg cgttcttacc acggctgaga aaaaatcact gttgaaacca
    20761 gaacatcagg gactggcgac ggaagtgatg tgccggatga acatcgcagg cgcgtccttt
    20821 gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg gctttggtct gctgggccac
    20881 ttgagcgaaa tgtgtcaggg ggctggtgtg caggcacgcg tcgactatga agcgatcccg
    20941 aaactccccg gtgttgaaga gtacattaag ttgggcgcag tacctggcgg cactgaacgt
    21001 aactttgcca gctacggtca tctgatgggt gaaatgccgc gtgaagtgcg cgatctgctg
    21061 tgcgatccgc aaacttctgg cggtttgctg ctggcggtca tgccggaagc agaaaatgag
    21121 gttaaaacta cagccgccga gtttggcatt gaactgacgg cgattggcga actggtgcca
    21181 gcgcgcggcg gtcgtgccat ggttgagatc cgttaattta atgcggttgt ttattgccga
    21241 aaaaccgagt ctggcgcgcg ccattgctga tgtcctgccc aaaccgcacc ggaaaggcga
    21301 tggctttatc gagtgcggta atggtcaggt ggtgacttgg tgtatcggtc acctgcttga
    21361 gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc tggaatcttg cggatttgcc
    21421 gattgtcccg gaaaagtggc aattacagcc acgaccctcc gtgaccaaac aacttaacgt
    21481 catcaaacgc ttcctgcatg aagccagcga aatcgttcac gccggggacc cggatcgtga
    21541 agggcaattg ctggtggatg aagtgctgga ctatctgcaa ctggcaccgg aaaagcgcca
    21601 gcaggtgcag cgttgcttga taaacgacct gaacccgcag gcggttgagc gggcgatcga
    21661 ccgtcttcgt tccaacagtg agtttgtgcc attgtgcgtt tctgcgctgg cgcgagcgcg
    21721 tgccgactgg ctgtacggca tcaatatgac ccgtgcgtat accattctcg gtcgcaatgc
    21781 cggttatcag ggcgtacttt ccgtgggacg cgtgcagaca cccgtgctcg ggctggtggt
    21841 gcgccgcgat gaagagattg aaaacttcgt ggcgaaagac ttctttgaag tcaaagcaca
    21901 tatcgtgaca cctgccgatg agcggtttac cgctatctgg caaccgagcg aagcgtgtga
    21961 accgtaccag gatgaagaag ggcgcttgtt acatcgtcca ctggcggagc atgtggttaa
    22021 ccgcattagt ggtcaaccgg ctattgtcac cagctataac gataaacggg aatcagaatc
    22081 cgcgccgctg ccgttttcgc tttcggcgtt gcagattgaa gcggcaaaac gcttcggtct
    22141 gagcgcgcag aacgtgcttg atatctgcca gaagctgtac gaaacgcaca agctaatcac
    22201 ttatccgcgt tccgattgtc gctatttgcc agaagaacat tttgccggac gccacgcggt
    22261 gatgaatgcc atcagcgttc atgcaccgga tctgttgccg cagccagtgg tagatccaga
    22321 tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg caccacgcca tcatcccgac
    22381 cgcacggagt tctgcgatca acctgacgga gaacgaagcg aaggtctata acctgattgc
    22441 ccgtcaatac ctgatgcagt tctgcccgga tgcggtgttc cgcaagtgtg ttatcgaact
    22501 ggacattgcc aaaggcaaat ttgtcgctaa agcccgtttt cttgctgaag caggctggcg
    22561 cacgctgtta ggcagcaaag agcgcgatga agaaaacgac ggcacgccat tgcctgtggt
    22621 ggcgaaaggc gatgagttgc tgtgtgaaaa aggtgaagtg gtagagcggc aaacccagcc
    22681 gccgcgccat tttaccgatg caacactgct ttcggcgatg accgggatcg cgcgctttgt
    22741 gcaggacaaa gatctgaaaa aggtccttcg tgcgaccgat ggtctgggga cagaagcaac
    22801 ccgtgccggg attattgaac tgctgttcaa gcgtggtttt ctgaccaaaa aagggcgcta
    22861 tatccactcc accgacgccg gaaaagcgtt attccattcg ctgccagaaa tggcgacgcg
    22921 accggacatg accgcgcact gggaatcggt gctgacgcaa atcagcgaaa agcagtgtcg
    22981 ctatcaggac tttatgcagc cgctggtggg gacgctatat cagcttattg atcaagccaa
    23041 acgtacgccg gtgcggcagt ttcgcggcat tgtggctccg ggcagtggtg gcagtgctga
    23101 taagaaaaag gctgcaccgc gtaaacgtag tgcgaaaaaa agtccgccag cagatgaagc
    23161 cggaagcggg gcgatagcgt aagcgagcga atctttcgtg ctattcgagt catattctga
    23221 aatatccagc ggatcaagaa aattcgttgg atattttttt gcatggataa aattatcgcc
    23281 tctaaagtat gtaataacag ggaatgtgat gaaaaaagtt ttattgcaaa accatcctgg
    23341 gagcgagaag tattctttta atggctgggg aatatttaat agtaattttg aacgggtgat
    23401 taaagaaaat aaggccatgc tgctttgtaa gtgggggttt tatttaacat gtgttgtcgc
    23461 tgtaatgttt gtattcgcag cgataacatc caacggtttg aatgaaagag gcctgattac
    23521 cgcgggatgc tcttttcttt atctattaat tatgatgggg cttattgttc gggccggttt
    23581 taaagcaaaa aaagaacaac tgcattatta tcaggctaaa ggtattgagc cgctcagtat
    23641 cgagaagtta caggcgctac aattgatcgc accttatcga ttctatcata agcaatggtc
    23701 tgaaacgcta gagttctggc cgcgaaagcc tgaacctggc aaagatacct tccaatatca
    23761 tgtgcttcct tttgactcga tcgatatcat cagtaaaaga cgcgagtctt tagagaatca
    23821 atggggtatc gaagatagcg aaagttattg tgccttaatg gagcattttc tttctggcga
    23881 ccatggagcc aataccttta aagcaaacat ggaggaagcc ccagagcagg ttatcgcctt
    23941 gttgaataaa tttgctgttt ttccctcaga ctatatctct gattgcgcta ataatcgctc
    24001 cggtaaatcc tcggcgaagc taatatgggc ggcggaatta tcatggatga tctcgatatc
    24061 aagcacagct tttcaaaacg ggacaattga agaagaactg gcctggcatt atataatgct
    24121 tgcttctcga aaggcgcacg agttgttcga aagcgaagaa gattatcaaa aaaatagtct
    24181 aatgggattt ctttactggc atatctgctg ctatcgcaga aagttaacgg atgctgaact
    24241 tgaagcgtgt tatcgttacg acaagcagtt ttgggagcac tacagtaaga aatgccgttg
    24301 gcccataaga aatgttccct ggggagcatc atccgttaaa tactcataaa cgcctgaaat
    24361 tttgccgggg gcgctgcgct tgaccggcct acattagagc gtaactgcaa tgaattgtgc
    24421 ccatctgtag gcctgataag cgtagcgcca tcaggcattt acaacttaaa tcacaccctg
    24481 cgccagcatc gcatcggcaa ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt
    24541 ggtttgctca ccttcaccac catgctcaac acaggcatgg tggatatcca gcatgatgtg
    24601 atgcaaacgt gcgtcaactt tctcggcttt ccagcccagg cgcgcagcgt tttgtgccat
    24661 ttccaggccc gacgttgcta caccgccagc attagccgct ttacccggtg caaacagtac
    24721 gcctgcctgc tggaacagtt cagtcgcttc gatggtggtc ggcatatttg ccccttcagc
    24781 gacggcttta acgccattgg cgataagctg atgcgcggcg tcaacatcca gttcgttctg
    24841 ggtggcgcaa ggcagggcga tatcaaccgg tacagaccac ggctgttggc cttcgagata
    24901 gaccagacca aattctttgg cgtaatctgc cactcgacca tcgcggctgg ctttgatttc
    24961 gataagacgt gccagtttct ctttcgtgaa tccgctttca tcaactacag tgccgctgga
    25021 gtctgacgca gtgatcacac gagcaccaaa ttccatcgct ttttcgatag cgtactgggc
    25081 gacgttgccg gagccagaaa cggaaacgct catcccttca aaacccatac cgtggcgttt
    25141 taacattgct tctgtgaaat aaaccagacc gtagccggta gcttgcgaat aagactaccg
    25201 ccaaatgaaa ggcccttacc ggtgaagacg caggcggtat tgttggagag ctttttcatc
    25261 atccccgcca taaagccgac ttcacgacca ccaacgccga tatcacctgc cggaacgtcg
    25321 gtatccgcgc ccaggtggcg atacagttca gtcatcagcg tctggcaaaa acgcatcact
    25381 ttaccttcgc tttttccttt cggatcgaaa tcgctgccgc ctttaccacc gcccatcggc
    25441 agggtagtca gggcattttt gaaggtttgt tcaaagccga ggaatttgag aatggaaagg
    25501 ttaactgacg gatggaagcg cataccgcct ttgtacgggc cgatggcaga gctgaactgc
    25561 acacgccatg cacggttgac ctgtatctgg ttgcgatcat caacccatac cacgcgaaac
    25621 tggatcacgc gctccggttc aaccagacgc tccagtaatg acatctggcg atattttgga
    25681 ttttgttcaa gaaaaggcca gagtgtggtc attacttcac gaacggcttg cgcgaactcg
    25741 gtttgattcg ggtcgcgctt ttggacatgg ttgaggaatg actccagaga atatgtctga
    25801 tccatagata taaaaacctt atatattaat acgattgtgc ttatgttgtt tatgtcatgt
    25861 gcttttgcag ttttcgacta taccatcaag aatgtgacgg taagcaagaa aaaaatgacc
    25921 caggaaagca aattaataac gagagtaatc tcataaccaa atgcgataac tcagacgcta
    25981 aagttttgcc acccggctac cgttatcagt agcagagaca ataacgttaa ggagttccct
    26041 gtgagtcgag cattgttcgc cgtagtatta gcatttccat taattgcact ggctaacccg
    26101 cattatcgtc cggacgtgga ggtaaacgta ccgccagaag tgttcagttc aggcgggcag
    26161 tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc aaaattattc tgaaggggcg
    26221 gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg atgacaaaac ccttagcact
    26281 aatccgctgc tctggcggcg cgtaaagcca taaacgcctc taataatgga atgtcagcag
    26341 gggccagcgg atattgcagc gcctcttcag gtgagcacca gaccagcgcc tgatgttcat
    26401 gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc atgaagatgg ataatccggc
    26461 ccgaaacttc tcgctgatgg ctggcaacat actcacccac agttgcttcg atgtccaatt
    26521 cttcgtttaa ctcacgcacc agcacctgct gctggctttc atccagctcg actttacctc
    26581 cggcaaactc ccataatccc gcctgatcgc tctgggcggg gcgttgcgcg agtaaaattt
    26641 tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat tttcataaat tacacacggg
    26701 cagataagcg aaaaatgcca taatatccgc actgttgctc aatcagaaag gctttatatt
    26761 taaggagtgc ctgtgctaga ccgccatctt catccccgga ttaaaccgtt gttgcatcag
    26821 tgcgtgcggg ttcttgataa accgggcatt acgccagatg gtttaacatt agtgggattt
    26881 gccatcggcg tgctggctct gccgtttctg gcgctgggct ggtatctggc ggcgctagtc
    26941 gtcattttgt tgaacaggct gcttgatggt ctggacggtg cgctggcgcg gcgcagaggg
    27001 cttaccgacg cgggcggctt tcttgatatt tctctcgatt ttctctttta cgcgctggtg
    27061 ccgtttggct ttattcttgc tgcaccggag caaaatgcgc tggcaggcgg ctggttgttg
    27121 tttgcgttta tcggcacggg cagcagtttt ctcacctttg ctgcactggc agcaaaacat
    27181 cagattgata accccggtta tgcacataag tcgttttact atctgggggg attaaccgaa
    27241 ggcacagaaa cgatcttact gtttgtgctg ggatgtttat tccctgcatg gtttgcgtgg
    27301 tttgcgtgga tcttcggtgc gttgtgctgg atgacgacct ttacgcgggt gtggagtggg
    27361 tatctgacgc tgaagtcgct ccagcgtcag taattgcaat gttatttgct actgcccggg
    27421 ccgcgttcac cggttgcaac cggatttttt ggatcgctgc tccattcgta ccagccgccg
    27481 tcatacacgg aaacattctt ccaacccatg gcgcgtgcgt acataaaggt ttcggacgcg
    27541 cgccagccgg tgccgcagta gaatgaaact tgctgatctg gtttgatatt ccatgctttc
    27601 cacatagcgg taatatcatc ggcgctacgc atggtgccat ccgggttata gaaatcttcc
    27661 atatgcgtcg agtcgctacc agcgtgtccc caacgtgctc cggctatttc accttttggt
    27721 ttaatatagc tgtaaccgct ggtcgtaccg ataaattctg gccacgaacg aatgctcacc
    27781 agcgatgcat cctggcgatg cagcagtcca cgggcctgtt ccatatcgag catcaactgc
    27841 ggttgtgccg ggatcttcac gccgaaatcc ggttccgctt tcactttcgg tggcgttcct
    27901 cgctcaacag gcagtcccgc gtcggaccag gtttgccagc cgccatccag cagacgcaca
    27961 tctttcacgc ctgcgtagag cataatttgc gccacacgcg ctccagcgta tacgtcacgc
    28021 ccgtacagaa tgaccgtggt gtcatggcga atgccgtgtt ttgccagcat cgcttttagt
    28081 tgttcatcag aaactttgtt ccacagcggt tcgctttcca cttcgttggt atcgatgtag
    28141 tcagcgccag gaatatggct gataaggtaa agcttaggag cgccctaggc cgcttcaatg
    28201 actttccagt caccgacagg tttcgccgta acctctttac cttgttgcag gtcatgcagc
    28261 cattgcggat aaaccagctg ctcaaagtgc ggcagttttt gtagacggga aggttcgctt
    28321 agcgcgtcac tcagaatgga gatatgcgta aaacctgctt tttgcagtcg cgttttgacg
    28381 gcgtcgacat ctttgccatt accgtacagc gccaccgggg catcggcttt caggttatgt
    28441 tgcttgatcc acgcgttgag ctgttcggtg ctcattttgt cgagccagct agcagagagg
    28501 tttaaggcgg caggttcatg accagaaggg ccatttaagg tttgtggcca gccgttataa
    28561 aacgcgctgg ggcgagtatc tatcgctttg ccattttgtt gctgaagttg gtcaagtgta
    28621 agaggcttcg ccagttcagc ggcccacgaa gaagcgcaag ccagccctaa agccattgcc
    28681 agcgcggtca tttgagaaac acgtttcatc gaaaatcccg acattgtagt tttgagtgcg
    28741 ccattctgaa ctgacgcatt gagaaaaact ttgcgttatc gcagtttgtt gtaattttct
    28801 gaccactgcg ccatatccag aacagaacta tcagcaggaa catcctggag atcgtgcgtt
    28861 acctgaacga cggggatcgc cagggcgcga acttcgctga acacccactg gcgaaaatta
    28921 tcgcgcagag ccacatcaag acggctgaat ggctcatcca ggagtaacgc ttttggctgg
    28981 gcgagaaggg cgcgtagtag agcaacgcgc gctcgctgac cgccagacaa agtggcagga
    29041 tcctgatgga aagctccgtc aaggcctgaa cgctcaagtg catcattcac ggcattacgt
    29101 cgggcattcc ccttaagtgt cgccggtagc gccagcagta aattttgccc gacgctgaac
    29161 tggtcaaata acagtgcatc ctgaaaaaga atgccaatct gacgctgtgc ggtgggtagc
    29221 atgtcaatcc gttgctcatt gagccatagc tcacctgtac aagaaaactg tccggccagt
    29281 gcaccaatca tccatgaaaa cagagtggat tttccacagc cagacggccc cattaacgtg
    29341 acgatgtcac ctttatcaac cgtaaagtta acgtttgtca gcaagcggct ttctggtaaa
    29401 cgtagcgaaa catttttcac gcagagcatt agcggattcc ttgtctgaca taacctaccc
    29461 attttgcgac taacgcggtc agggcaaaaa taataagcgg taacaacagt tgccataacg
    29521 cctgggcggc gagaataccg ttgctgccgc cgctgcttaa tgccaccgcc tcagtggtga
    29581 gcgtcggaaa acgccccgcg cccagccaca gcgtcggcat atactgcgca atactgactg
    29641 aaaatcccac cgcgaaggca gtcagcacag ggcgcaacat gagtgggcat ttcacgtaaa
    29701 agaagatttt ggcccgcgac cagcccagtg tttgcgcaat caaaattaac cgtgaatcaa
    29761 tgcgctgcca ggcgggttgc aggataaaca gcatccacgg cattacccac agcagatgcc
    29821 cccagaccac cgccgtccag cttccatcca ggttcagcca tagcgccagc gtgtactggc
    29881 ccgccaccag tggcagagcg ggcaatagaa tgggcaacca tagccacaac tggcgacgct
    29941 gtgcccccca ttccagccac agcagtaaca ggagcaaagc gatgaatgtt gctaccagcc
    30001 ccattgtcag actgttgatg agtgcttcac tattgatcgt cgactgatcc gcgaggatcg
    30061 ccagcagaac cacacacagc acaccggtta agggtaaaaa actcgccagc gtagtgcctg
    30121 gtaataaagg tgtggcaggc ttgcgaatgc catctacgcg gggaatagtg cgccgccagc
    30181 tgcgccaaaa cgtaggcggc gagtaacagc atcagcagca gactggcgag tgcgcctttc
    30241 gtttgttgat ccgcgtcgcc ctgagttaac cactgccagc taattactgc cagcgtcggt
    30301 ggattacctg gcccaagaat aatcgccaca tccacgaccg aaagcgacca ggcaacaatc
    30361 gccagcatcg ccattgccag cgaaggcgcg acggagggca acagcagcca gttcaggcat
    30421 tgccagcggc tgtagcccag cgaatccagc acaatgacct gctgcaacag ccgtttttcg
    30481 ctcaatactg ccactaagat ccacagcaga aatgcgcttt ctttcactgc aagggtgagg
    30541 cccagaccga tccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca
    30601 gccctccgtc agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga
    30661 gcagccacgg cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa
    30721 tcaccagcag ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg
    30781 ccagtaatgc ctgcggtaac tgtggatcgg caaataacgc ctgccagcgt gtcaacgaca
    30841 acgcaggcga tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca
    30901 tcgcccacag caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc
    30961 attcacccat cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga
    31021 ttgcaatatt tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgcc
    31081 ccagacaacg ggatctgctt tacgcagttg cgcatcgggt gagagcagga aattggcaac
    31141 taccttcgcc gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc
    31201 aatcatcccc tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat
    31261 tttttgctgc gcatgtgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag
    31321 cagagcatcc atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg
    31381 atgcagtgca tcaagatatt gctacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc
    31441 cggcgcttct tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa
    31501 cgccgtg
//
LOCUS       DAJVPP010000043        31326 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVPP010000043 DAJVPP010000000
VERSION     DAJVPP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.043"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..944
                     /gene="espL1"
                     /locus_tag="OQM34_001926"
                     /pseudo
     CDS             1..944
                     /gene="espL1"
                     /locus_tag="OQM34_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1116..1223
                     /locus_tag="OQM34_001927"
     CDS             1116..1223
                     /locus_tag="OQM34_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452394.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1276..2034)
                     /locus_tag="OQM34_001928"
     CDS             complement(1276..2034)
                     /locus_tag="OQM34_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1452395.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2321..3250
                     /gene="pfkB"
                     /locus_tag="OQM34_001929"
     CDS             2321..3250
                     /gene="pfkB"
                     /locus_tag="OQM34_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1452396.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3351..3641
                     /gene="ghoS"
                     /locus_tag="OQM34_001930"
     CDS             3351..3641
                     /gene="ghoS"
                     /locus_tag="OQM34_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1452397.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3747..4607
                     /locus_tag="OQM34_001931"
     CDS             3747..4607
                     /locus_tag="OQM34_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1452398.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4648..5184)
                     /locus_tag="OQM34_001932"
     CDS             complement(4648..5184)
                     /locus_tag="OQM34_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1452399.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5331..5999
                     /gene="hxpB"
                     /locus_tag="OQM34_001933"
     CDS             5331..5999
                     /gene="hxpB"
                     /locus_tag="OQM34_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS1452400.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6162..6752
                     /locus_tag="OQM34_001934"
     CDS             6162..6752
                     /locus_tag="OQM34_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1452401.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6885..8275
                     /gene="tcyP"
                     /locus_tag="OQM34_001935"
                     /pseudo
     CDS             6885..8275
                     /gene="tcyP"
                     /locus_tag="OQM34_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8279..9095)
                     /locus_tag="OQM34_001936"
                     /pseudo
     CDS             complement(8279..9095)
                     /locus_tag="OQM34_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9372..9635)
                     /gene="cedA"
                     /locus_tag="OQM34_001937"
     CDS             complement(9372..9635)
                     /gene="cedA"
                     /locus_tag="OQM34_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCS1452402.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9818..12079
                     /gene="katE"
                     /locus_tag="OQM34_001938"
     CDS             9818..12079
                     /gene="katE"
                     /locus_tag="OQM34_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCS1452403.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12126..12884)
                     /gene="chbG"
                     /locus_tag="OQM34_001939"
     CDS             complement(12126..12884)
                     /gene="chbG"
                     /locus_tag="OQM34_001939"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCS1452404.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12897..14249)
                     /gene="celF"
                     /locus_tag="OQM34_001940"
                     /gene_synonym="chbF"
     CDS             complement(12897..14249)
                     /gene="celF"
                     /locus_tag="OQM34_001940"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1452405.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKKPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14354..15196)
                     /gene="chbR"
                     /locus_tag="OQM34_001941"
                     /pseudo
     CDS             complement(14354..15196)
                     /gene="chbR"
                     /locus_tag="OQM34_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15204..15554)
                     /gene="chbA"
                     /locus_tag="OQM34_001942"
     CDS             complement(15204..15554)
                     /gene="chbA"
                     /locus_tag="OQM34_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCS1452406.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15613..16962)
                     /gene="chbC"
                     /locus_tag="OQM34_001943"
     CDS             complement(15613..16962)
                     /gene="chbC"
                     /locus_tag="OQM34_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCS1452407.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17047..17367)
                     /gene="chbB"
                     /locus_tag="OQM34_001944"
     CDS             complement(17047..17367)
                     /gene="chbB"
                     /locus_tag="OQM34_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS1452408.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17666..18004)
                     /gene="osmE"
                     /locus_tag="OQM34_001945"
     CDS             complement(17666..18004)
                     /gene="osmE"
                     /locus_tag="OQM34_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1452409.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18206..19033
                     /gene="nadE"
                     /locus_tag="OQM34_001946"
     CDS             18206..19033
                     /gene="nadE"
                     /locus_tag="OQM34_001946"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1452410.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19263..20150
                     /gene="cho"
                     /locus_tag="OQM34_001947"
     CDS             19263..20150
                     /gene="cho"
                     /locus_tag="OQM34_001947"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1452411.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20110..20685)
                     /gene="ves"
                     /locus_tag="OQM34_001948"
     CDS             complement(20110..20685)
                     /gene="ves"
                     /locus_tag="OQM34_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1452412.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20888..21373)
                     /gene="spy"
                     /locus_tag="OQM34_001949"
     CDS             complement(20888..21373)
                     /gene="spy"
                     /locus_tag="OQM34_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1452413.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21703..22671)
                     /gene="astE"
                     /locus_tag="OQM34_001950"
     CDS             complement(21703..22671)
                     /gene="astE"
                     /locus_tag="OQM34_001950"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1452414.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22664..23997)
                     /gene="astB"
                     /locus_tag="OQM34_001951"
                     /pseudo
     CDS             complement(22664..23997)
                     /gene="astB"
                     /locus_tag="OQM34_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(23994..25472)
                     /gene="astD"
                     /locus_tag="OQM34_001952"
     CDS             complement(23994..25472)
                     /gene="astD"
                     /locus_tag="OQM34_001952"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCS1452415.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25469..26503)
                     /gene="astA"
                     /locus_tag="OQM34_001953"
     CDS             complement(25469..26503)
                     /gene="astA"
                     /locus_tag="OQM34_001953"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1452416.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26500..27720)
                     /gene="astC"
                     /locus_tag="OQM34_001954"
                     /pseudo
     CDS             complement(26500..27720)
                     /gene="astC"
                     /locus_tag="OQM34_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28166..28972
                     /gene="xthA"
                     /locus_tag="OQM34_001955"
     CDS             28166..28972
                     /gene="xthA"
                     /locus_tag="OQM34_001955"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1452417.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29139..29848
                     /locus_tag="OQM34_001956"
                     /pseudo
     CDS             29139..29848
                     /locus_tag="OQM34_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29853..30530
                     /gene="ydjY"
                     /locus_tag="OQM34_001957"
     CDS             29853..30530
                     /gene="ydjY"
                     /locus_tag="OQM34_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCS1452418.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30545..31252
                     /locus_tag="OQM34_001958"
     CDS             30545..31252
                     /locus_tag="OQM34_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1452419.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31252..>31326
                     /locus_tag="OQM34_001959"
     CDS             31252..>31326
                     /locus_tag="OQM34_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1452420.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYG"
BASE COUNT         8067 a         8031 c         7370 g         7858 t
ORIGIN      
        1 ggctgatagg tatgttcgca actgtgcaat tgctattctg cccctattac ctgtgggagt
       61 tccaggggct attttcgcgg gtattgcatc acgaatgcca tttgccccta tacatccatc
      121 ggcaatgttg ttaataatgg ctacaggcca gactcaacag cttattacat tattcaaaca
      181 gttgcccata ctccctgaaa aagaaatcat tgaaataata actgcgcaga atagcgttgg
      241 tacacctgct ttatttttgg ctatgatgaa cggacatact gacaacgtga aaatatttat
      301 gcaagaaatt cagtcactgg tagataatca catcattcat gaagataatc tggttaaatt
      361 actgcaaact aaaagtgcta acgaaacacc tggactttat atctccatgt tgtatggatt
      421 cgatgaaata atcgatatct ttctgaatac attaaccact ccaatagcac taagagcttt
      481 taaacaaaaa actggtgatg agtattttag ccatgaaaat acatgatggt gagccaggat
      541 tatacgccgc aatggaaaat aatcaccctt tgtgtgtcac acggttcctc tctaaaatta
      601 atggcatcgc ctttaaatac aagttgagca aagctaacat catggattta ttaaaaggag
      661 ctacagcaca gggaacccct gcattataca tcgccatgag caagggtaat gaagacgtcg
      721 tgttatctta tatatcgacg ctgggtgctt ttgcaaaaaa acattctttt agtcaacatc
      781 agttatttac actattggcc gctaaaaatc atgacaacat gtcagctgtt catatagcca
      841 ttcatcataa gcattataaa actgtagaaa catattatgc tgctataaat gtcatcagcc
      901 aaagcctgag ttttagtgct gatgaaataa agacgtattt ataaccagct atttacgata
      961 tatatcgata cataatttta tttttttcat aaaacgcaga aacagtgacc atgtgcacaa
     1021 cgccacccga cggctgtaat aacaaaaaat cccttgatgc ctgtcccttt tgttacactc
     1081 cgttatcacg cacaagagat atgcaggaca ctggtatgcc gactaaacgc tttgataaaa
     1141 aacactggaa gatggtggtg gtgctactgg caatctgtgg cgctatgttg ttgctacgtt
     1201 gggcagcaat gatttggggc tgagcagtga caaacgaaga cagccagacc gcataacacg
     1261 gtccggcaga taattttaca tgctgtagcc cagcgataag gttgtccgac ggtcggtatg
     1321 ttccggtgca gactctggag gttcagagtt ccatgtgacg ttgtaagcca ccttcagtcc
     1381 aaaatgttca ttaatagcaa catttaatgc gctttcggag ttcagcgttg tgtcttccgc
     1441 gccaaagacc gaaacaccct gcgtaaattt agcgttgtca gtcaactgcc atgcataagc
     1501 cccggaggca tagcccagcg gctgggtttc actggcattg tcggtatatt tgtcgtaacg
     1561 cacacctgga ccgaattcaa agcggaaact gtgtaccggg ccattcaaaa actgacgacc
     1621 ataacccgcg gtcaacacat cgcgctctcg atagccgtta taacggtctg tcagccagct
     1681 tgcctgacca aataaatagt cataatcagt taaattaaag cggctacgcc cgcccgccgc
     1741 atatttttct gaagaacgct catcattaga agaagtatta ctggcgttcc cccacagcga
     1801 ccaggcagtg gtttgtccat accaggtcat ggtggtgtca gccgtaagtg aggagctttt
     1861 cgtattgcct gattgtgcaa gatatcctgc gttcagatta ccttcgaaag gttttttagc
     1921 gctggcagga tcgtccatga cagtaaaaac ggaatcatcg gcagctgcat tcagtgacgc
     1981 aaacatgccc cccgccaaca taacgatggc aggaactgtc ttcaaaagct tcatttatca
     2041 agagtccgta caacaaaaaa agagaccatc gcggtcccgg aaactttctt aaggatcaaa
     2101 gattagcgtc cctggaaagg taacgaatta taaaaaggcg cgaataactt agcaatgtat
     2161 tcttatttca ttttttgaat aagcatgtgg cgaaaacaga tttttattta tatatattta
     2221 tctgcaaaat tttaaataaa gctctaataa atcatattgt taatttcttc actttccgct
     2281 gattcggtgc cagactgaaa tcagcctata ggaggaaatg atggtacgta tctatacgtt
     2341 gacacttgcg ccctctctcg atagcgcaac aattaccccg caaatttatc ccgaaggaaa
     2401 actgcgctgt accgcaccgg tgttcgaacc cgggggcggc ggcatcaacg tcgcccgcgc
     2461 cattgcccat cttggaggca gtgccacagc gatcttcccg gcgggtggcg cgaccggcga
     2521 acacctggtt tcactgttgg cggatgaaaa tgtccccgtc gctactgtag aagccaaaga
     2581 ctggacccgg cagaatttac acgtacatgt ggaagcaagc ggtgaacagt atcgttttgt
     2641 tatgccaggc gcggcattaa atgaagatga gtttcgccag cttgaagagc aagttctgga
     2701 aattgaatcc ggggccatcc tggtcataag cggaagcctg ccgccaggtg tgaagctgga
     2761 aaaattaacc caactgattt ccgctgcgca aaaccaaggg atccgctgca tcatcgacag
     2821 ttctggcgaa gcgttaagtg cagcactggc gattggtaac atcgagttgg ttaagcctaa
     2881 ccaaaaagaa ctcagtgcgc tggtgaatcg cgaactcacc cagccagacg atgtccgcaa
     2941 agccgcgcag gaaatcgtta atagcggcaa ggccaaacgg gttgtcgttt cccttggtcc
     3001 acaaggagcg ctgggtgtcg atagtgaaaa ctgcattcag gtggtgccac caccggtgaa
     3061 aagccagagt accgttggcg ctggtgacag catggtcggc gcgatgacac tgaaactggc
     3121 agaaaatgcc tctcttgaag agatggtccg ttttggcgta gctgcgggga gtgcagccac
     3181 actcaatcag ggaacacgtc tgtgctccca tgacgatacg caaaaaattt acgcttacct
     3241 ttcccgctaa caaaaacatt cccccagcat tgggggaatc accaccaacc tgtcggcaac
     3301 gcgtttctcc gactatgctc aaaagtcatg tgataacaaa ggggtgaact atggccagtg
     3361 gcgatcttgt ccgttatgtc ataaccgtca tgttgcatga ggatacattg actgaaatta
     3421 acgagttgaa taattacctg actcgcgacg gttttttgct caccatgacg gatgatgagg
     3481 gaaatatcca tgagctgggg actaacactt ttggacttat cagtacccaa agtgaagaag
     3541 aaattagaga actggtttcg gggcttaccc aaagtgcaac cggcaaagat cctgaaatca
     3601 ccatcacgac ctgggaggaa tggaatagca acagaaaata aatggttttt gggcaataat
     3661 cagtctgtgg tgtgcgttag ctcgtgtttt tacaccgcat tcttgcgcta accttatgat
     3721 ctggcagaca acatgggaga gacatcatgt ggcaggcaat cagtcgtctt ttgagcgagc
     3781 agttaggtga aggcgaaatc gaactgcgta atgaactgcc tggcggagaa gtccatgccg
     3841 catggcattt gcgctatgca ggacatgact ttttcgtcaa atgtgatgaa agggaactgc
     3901 ttcccggttt taccgccgaa gccgaccaac tggagttact gtcgcgtagt aaaaccgtca
     3961 ccgtgcctaa ggtttgggca gtaggcgctg accgtgacta cagttttctg gtgatggatt
     4021 atctcccacc tcgtccgctg gatgcgcata gcgcatttat tcttggtcag caaattgcgc
     4081 gtttacatca atggagtgac caaccacaat ttggcctcga ttttgataac tcgctctcca
     4141 caactccaca gcccaacacc tggcaacgtc gctggtcaac gttttttgct gaacaacgga
     4201 ttggctggca gttggaactg gcagcagaga aagggatcgc tttcggcaat atcgacgcca
     4261 tcgtcgagca tattcagcag cgtctggcct cgcatcaacc gcagccttct ctgttgcacg
     4321 gcgatttatg gtccggcaac tgtgcgctgg gtccggatgg cccgtacatt ttcgaccctg
     4381 cctgcgactg gggtgaccga gagtgcgacc tagcgatgtt accgctgcat actgaacagc
     4441 cgccacaaat ctatgacggc tatcagtcag tatccccgct acctgccgat ttccttgaac
     4501 gtcaaccggt ttaccaactc tacacgctgt taaatcgtgc aagattattt ggcggtcagc
     4561 atttggttat tgctcagcag tcattggata gattattagc agcatgatat gggttgagga
     4621 taatggccgc tccgtgcggc cttttgatta aataaaccca agcagagaaa agaacacata
     4681 acctgcagca atacaaataa ccggcaggat atatagtgag aaaaactgca ggaaaatagt
     4741 atggtgcgga acaacaatac gggactcaat ttgttcacgc gttagcccct cttccccttt
     4801 ggctttttcc agaatgagtt gatcttcaac accttcccgc aagaagcgcg cctggcgact
     4861 catccgggca ccggaatcct gcaacgccag tccgacaaaa atcaggatga aaatcaccca
     4921 gaacataacg ttcacaccac cattaaaatt aggggtcggg gagttatacc agaacagatt
     4981 caaaaaaggc gtattcgcct gcatcatatc gatcatgaca tgagcaaagt cgagcatgac
     5041 cgcattaatg ccttcctggt tttcctgccg ggtattcata aatttcagca aagaaatcaa
     5101 tgtagagatc aatgcaggta taaaaatcac ccaacccaaa atccttttca aaacagcaat
     5161 gcgtccagct tgttgatacg tcatgagttc tccttgatta agacgcgtcg tttcacttag
     5221 tttacctgta gatatctgtt ttcgcccatt ctttaaaggc gatatgatag gcgcttaatc
     5281 ataagcacgg cttaatacct tacacataat gctctaaagg agaggttgta atgtcaaccc
     5341 cgcgtcagat tcttgctgca atttttgata tggatggatt acttatcgac tcagaacctt
     5401 tatgggatcg agccgaactg gatgtgatgg caagcctggg ggtagatatc tcccgtcgta
     5461 acgagctgcc ggacacctta ggtttacgca tcgatatggt ggtcgatctt tggtacgccc
     5521 ggcaaccgtg gaatgggcca agccgtcagg aagtagtaga acgggttatt gcccgtgcca
     5581 tttcactggt tgaagagaca cgtccattat taccaggcgt gcgcgaagct gttgcgttat
     5641 gcaaagaaca aggtttattg gtgggactgg cctccgcgtc accactacat atgctggaaa
     5701 aagtgttgac catgtttgac ttacgcgaca gtttcgatgc cctcgcctcg gctgaaaaac
     5761 tgccttacag caagccgcat ccgcaagtat atctcgactg cgcagcaaaa ctgggcgttg
     5821 acccactgac ctgcgtggcg ctggaagact cggtaaatgg catgatcgct tctaaagcag
     5881 cccgcatgcg ttccatcgtc gttcctgcgc cagaagcgca aaatgatcca cgttttgtat
     5941 tagcaaacgt caaactctca tcgctgacag aactcaccgc aaaagacctt ctcggttaat
     6001 gaccaggggc agtgatcacg ctgcccctgg ttctttatct gaatttgcat tcaactgacg
     6061 gattaatcgt caatttaaga gaaagagtta caccgtcacc acttccgtgc actgtataaa
     6121 aatcctatac tgtacgtatc gacagtttag tgagttttat catgacggcg gaaggtcacc
     6181 ttctcttttc tattgcttgt gcggtatttg ccaaaaatgc cgagctgacg cccgtgctgg
     6241 cacagggtga ctggtggcat attgtccctt ccgcaatcct gacgtgtttg ttaccggaca
     6301 tcgatcaccc aaagtcgttt cttgggcagc gattaaaatg gatatcaaaa ccgatcgccc
     6361 gcgcttttgg gcatcgtggt tttacccaca gtctgctggc ggtatttgcg ctgctggcaa
     6421 ccttttacct taaggttccg gaaggctggt tcattccggc tgatgcgcta caaggaatgg
     6481 tgctgggtta tttgagccac atacttgccg atatgctgac acccgccggt gttcccctgc
     6541 tctggccatg ccgctggcgt ttccgcttgc ctatcctggt tccccaaaag ggcaaccaac
     6601 tggaacgttt tatctgcatg gcattatttg tctggtcggt atggatgccc cattcattac
     6661 ccgagaacag cgctgttcgt tggtcatcgc aaatgatcaa taccttgcag atccagtttc
     6721 atcggcttat taagcatcag gttgaatact aaaaaggcaa aaatcacctt tctggaataa
     6781 gcaattccat ttgaatataa gagccagctc acagttctgt taatcttgcg ccaacactat
     6841 gactgctacg cagtgataga aataataaga tcaggagaac ggggatgaac tttccattaa
     6901 ttgcgaacat cgtggtgttc gttgtactgc tgtttgcgct ggctcagacc cgccataaac
     6961 agtggagtct ggcgaaaaaa gtgctggtgg gtctggtgat gggtgtggtt tttggccttg
     7021 ccctgcatac catttatggt tctgacagcc aggtacttaa agattctgta cagtggttta
     7081 acatcgttgg taacggctat gttcaactgc tgcaaatgat cgttatgccg ttagtcttcg
     7141 cctctattct gagcgcggtt gcccgtctgc ataacgcatc tcagttaggc aaaatcagtt
     7201 ttctgaccat cggtacgctt ttgtttacca cgctgattgc ggcgctggtc ggtgtgctgg
     7261 tcaccaacct gtttggtttg acggctgaag gtctggttca gggtggtgca gaaactgcac
     7321 gtctgaacgc cattgaaagt aactatgttg gtaaagtctc tgatcagagc gttccgcagc
     7381 tggtcttgtc ctttatcccg aaaaacccgt ttgccgatct taccggagcc aatccgacgt
     7441 caattatcag cgtggtaatt tttgccgcat tcctcggcgt agctgcattg aaactgctga
     7501 aggatgatgc gccgaaaggg gaacgcgtct taaccgctat cgatacccta caaagctggg
     7561 tgatgaaact ggttcgcctt gtcatgcagt tgacccctta cggcgttctg gctctaatga
     7621 ccaaagtggt tgcaggttct aacttgcaag acatcatcaa actgggaagt ttcgttgtcg
     7681 cgtcctacct cggtctgctg attatgtttg ctgtgcatgg cattctgctg ggcattaatg
     7741 gcgtgagtcc gctgaagtac ttccgtaagg tatggcctgt gctgacgttt gcctttacca
     7801 gccgttccag tgctgcgtct atcccactga atgtggaagc acaaacgcgt cgtctgggcg
     7861 ttcctgaatc catcgccagt ttcgccgcct ctttcggtgc aaccattggt cagaacggct
     7921 gcgccggttt gtatccggca atgctgcggt gatggttgcg cctacggttg gcattaaccc
     7981 gctggacccg atgtggattg cgacgctggt cggtattgtt accgttagtt ccgcaggcgt
     8041 tgccggtgtc ggtggtggtg caactttcgc cgcactgatt gtgctgcctg cgatgggcct
     8101 gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg cttatcgaca tgggccgtac
     8161 ggcgctaaac gttagtggct cgatgacagc tggcacgctg accagccagt ggctgaagca
     8221 aaccgataaa gccattctgg atagcgaaga cgacgccgaa ctggcacacc gttaattctt
     8281 atgctggcaa cggtccgttt tgtatagggg ccgttgcttt actttttaaa tgttccatcg
     8341 cggcgatgca tactgactca tatttaaaca gatcgtcagc gaaaaaaatg tccaacttat
     8401 caatacattc ctggctcaat gaatgtgcct gtagtgagaa gcttaaaacc tcatacccga
     8461 gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagcaca ccacttccca
     8521 ttcccggaag atttagtgga ttaaaaagaa gattgatatt aagcattgat attatcgaag
     8581 gaaactttag gagactctca ttttttttga atcgcagcaa aaatgctccc ggcaggatgt
     8641 atataatttt tttcatagta tatagtcctt aaataatgaa taaccaacct attttttata
     8701 ttgatcaaat tcgtcatttt ttcgggtcgg aattcacagg taaaatccat cccaacaaaa
     8761 ttctcattaa tatctcttat ccgaaactca ctgccataaa gattattaat tatcactcta
     8821 tatttaacat tgtcctgcag atacgtatca aaactaagtg ctggcgctcc tagctttgag
     8881 gcatcatagt taatgttaga catataggca atcatacgat tgaagttcga ctctgctatt
     8941 tcatcgcagt cacattgacc cgttgatata aagaaagtga gtgccgccat ggtagactca
     9001 agcctttctt taaaagagtc gaaagccagg cttttaatat ttaaatcacc ataattactc
     9061 tgtattaagt ttgtagaaaa catctcccgc ctcatattgt taacaaaatt attatcttat
     9121 ttaaatctaa gtcatttaca atataagttt aagagcgacg ccacaggatg aactatcaaa
     9181 aataactcat catgattagc aaaacttaac cattttaaaa taaataaata attaaagaaa
     9241 aaagatcact tatttatagc aatagctcgt caaaggcagc tttttgttac aggtggtttg
     9301 aatgaatgta gcaacgaaat gcagaatttc aggtcctgta actcccggca aaaccgggag
     9361 gtatgtaatc cttactcagt cacttcccct tcctggcgga tctgatttgc ccaacgttgg
     9421 gcagattcag gcacagtaaa cgccggtgag cgcagaaatg actctcccat cagtacaaac
     9481 gcaacatatt tgccacgcag catccagaca tcacgaaacg aatccatctt tatcgcatgt
     9541 tctggcggcg cgggttccgt gcgtgggaca tagctaataa tctggcggtt ttgctggcgg
     9601 agcggtttct tcattactgg cttcactaaa cgcatattaa aaattagaaa aactgtagtt
     9661 tagccgattt agcccctgta cgtcccgctt tgcgtgtatt tcataacacc gtttccagaa
     9721 tagtctccga agcgggatct ggctggtggt ctatagttag agagtttttt gaccaaaaca
     9781 gcggcccttt cagtaataaa ttaaggagac gagttcaatg tcgcaacata acgaaaagaa
     9841 cccacatcag caccagtcac cactacacga ttccagcgaa gcgaaaccgg ggatggactc
     9901 actggcacct gaggacggat ctcatcgtcc agcggctgaa ccaacaccgc caggtgcaca
     9961 acctaccgcc cctgggagcc tgaaagcccc tgatacacgt aacgaaaaac ttaattctct
    10021 ggaagacgta cgcaaaggca gtgaaaatta tgcgctgacc actaatcagg gcgtgcgcat
    10081 cgccgatgat caaaactcac tgcgtgccgg taaccgtggt ccaacgctgc tggaagattt
    10141 tattctgcgc gagaaaatca cccactttga ccatgagcgc atcccggaac gtattgttca
    10201 tgcacgcggc tcagcagctc acggttattt ccagccatat aaaagcttaa gcgatatcac
    10261 caaagcggat ttcctctcag atccgaacaa aatcacccca gtatttgtta ggttctctac
    10321 cgttcagggt ggcgctggct ctgccgatac cgtgcgtgat atccgtggtt ttgccaccaa
    10381 gttctatact gaagagggta tttttgacct cgttggcaat aacacgccaa tcttctttat
    10441 ccaggatgcg cataaattcc ccgattttgt tcatgcggta aaaccagaac cgcactgggc
    10501 aattccacaa gggcaaagtg cccacgatac tttctgggat tatgtttctc tgcaacctga
    10561 aactctgcac aacgtgatgt gggcgatgtc ggatcgcggc atcccccgca gttaccgcac
    10621 catggaaggc ttcggtattc acaccttccg cctgattaat gccgaaggga aggcaacgtt
    10681 tgtacgtttc cactggaaac cactggcagg taaagcctca ctcgtttggg atgaagcaca
    10741 aaaactcacc ggacgtgacc cggacttcca ccgccgcgag ttgtgggaag ccattgaagc
    10801 aggcgatttt ccggaatacg aactgggctt ccagttgatt cctgaagaag atgaattcaa
    10861 gttcgacttc gatcttctcg atccaaccaa actgatcccg gaagaactgg tgcccgttca
    10921 gcgtgtcggc aaaatggtgc tcaatcgcaa tccggataac ttctttgccg aaaacgaaca
    10981 agcagcattc catccaggtc atattgttcc tggtctggac ttcaccaacg atcccctgtt
    11041 gcagggacgt ttgttctcct ataccgatac acaaatcagt cgtcttggtg ggccgaattt
    11101 ccatgagatt ccgattaacc gcccgacctg cccttaccat aatttccagc gtgacggcat
    11161 gcatcgtatg gggatcgaca ctaacccggc gaattatgaa ccgaactcga tcaacgataa
    11221 ctggccgcgc gaaacaccac cggggccgaa acgcggcggt tttgaatcat accaagagcg
    11281 cgtggaaggc aataaagttc gcgagcgcag cccatcgttt ggcgaatatt attcccatcc
    11341 gcgtctgttc tggctaagtc agacgccatt cgagcagcgc catattgtcg atggtttcag
    11401 ttttgagtta agcaaagtcg ttcgtccgta tattcgtgag cgcgttgttg accagctggc
    11461 gcatattgat ctcactctgg cccaggcggt ggcgaaaaat ctcggtatcg aactgactga
    11521 cgaccagctg aatatcaccc cacctccgga cgtcaacggt ctgaaaaagg atccatcctt
    11581 aagtttgtac gccattcctg acggtgatgt gaaaggtcgc gtggtagcga ttttacttaa
    11641 tgatgaagtg agatcggcag accttctggc cattctcaag gccatgaagg ccaaaggcgt
    11701 tcatgccaaa ctgctctact cccgaatggg tgaagtgact gcggatgacg gtacggtgtt
    11761 gcctatagcc gctacctttg ccggtgcacc ttcgctgacg gtcgatgcgg tcattgtccc
    11821 ttgcggcaat atcgcggata tcgctgacaa cggcgatgcc aactactatc tgatggaagc
    11881 ctacaaacac cttaaaccga ttgcgctggc gggagacgcg cgcaagttta aagcaacaat
    11941 caaggtagct gaccagggtg aagaagggat tgtggaagct gacagcaccg acggtagttt
    12001 tatggatgaa ctgttaacgc tgatggcaga acaccgcgtg tggtcacgca ttcctaagat
    12061 tgacaaaatt ccggcgtaaa tatggataag gtcacgtccc ctggggagtg accttaccac
    12121 cttacttaca catcaagata actacccaac cgataaccac gctcagcaat tgcatatttc
    12181 aacgacgctg atgtcagcac ttccagctcc gttaaccgtg ggaaacagta agcactctga
    12241 cggatagtat tatcgataaa cgccggatga cacatcacct ccagcgaagg atcaccccga
    12301 tggcttgcgt catccagcac ttgcaggaac agtgcctcgc taatctcttc accataaaac
    12361 gcgctactga atccctgcga actgcgcaga ttagccggta aatcaccagc gttagatagc
    12421 ggctgacggt cgatgcgcaa cgcaatcccc tcttcagccg caaacctggc aacaatcggg
    12481 aaaatctgcg ggaacatatg cacatgatga tggctatcaa gatgcgtagg tttgcgtcca
    12541 aatagctcaa tgaaacgcaa atactgactg gcaagctcct gagtaatttc ttccagcggt
    12601 aaagcatctt cttctgccaa ctgccagatc cattttccca gcacaccatc gcgggttaac
    12661 cccggcatag ctgtcagtgg cttgcccata gtaaggacaa agtgcatccc tatggccaga
    12721 ctcggttcat cacgactcaa ctgcaccgca tggtcaatag cctgcccatt caccagcgcc
    12781 gtcgtcgatg tgacaatccc attgcgacag gcctcgataa tgccgtagtt ctgacctttg
    12841 cttaagccaa aatcatcggc attaacaatc agtaagcgtt ccataatcag cctcggttaa
    12901 tgtgcttttt taagctctgc gatgcagtcg gcaaaatttg gcagccattt ctcgtgcgcc
    12961 agaatcatct cgcgtgccag cagctcagca tcgcgatcgg aatgcaccaa cggactaagg
    13021 tttagcgcca gtaacacatc gttaaattct ccgctaagtg cggcgttgct ggcagcaatc
    13081 tcgaagcctt taatggtgtg aatcagcccc atcactttat catcgaaatg cgtaatgcgc
    13141 ggatgtggcg tcgcgccatc gcgccccagc ttacaggtca tttctaccgc ccagtctgcc
    13201 ggaatattat caatatgccc atgatgcggg atattaacgt aatgttcagc ttgcttgtcg
    13261 ttgtagatag cgttgatcac ttcgcacgct gcatcagagt aataagcccc accgcgctgt
    13321 tccagttctt tcggcttaac tttcaactcc ggttttttat acagctcaaa aagttgtttc
    13381 tcgactttct gtactacctg cgctcgtgcg ccgcctttgt agtattcgcc catttcaata
    13441 gccagcatct ctttttgctt gaagtaataa agcaaataag aacacggcaa cagattcaga
    13501 gaacgaatta agccttcgct aaatggcaga tcgaaaatat ttttaacgcc agatactttt
    13561 aactgcccgg atgccacacc atcaagcaat tcggcaaagc gcgacttgcc atttaccagc
    13621 acatccttaa tgaacaccat atggttgagg ccaaacaaat cgatagataa atcatcgctg
    13681 tctttcagca tcagaacatc gtgaataaac atcttcatgc cgatcggaat attacacacg
    13741 ccgataaaac gtttaaatcc ggtatgacga taaacggctt cagtaaccat tcccgccggg
    13801 ttagtgaagt taatcaccca tgcattcgga caaagttctt cgacatcttt tacgatgtca
    13861 aaaatcaccg gaatggtacg cagaccttta aacaaaccgc ccgcgccgtt ggtttcctga
    13921 ccaagataac catgacttaa tggaatacgt tcatccagtt cacgcgccgg taattggcca
    13981 acgcgcagtt gggtagtaac gaaatcagca tctttcaatg cttcgcggca atccagcgtt
    14041 ttataaagct tcatcgggac gccagcgtta tcaatcatcc gttggcagag atcgaaaata
    14101 atatccagtt tagctttgcc accctcgaca tccaccagcc ataattcgct gaccggcaat
    14161 tcgtgataac gcttaataaa tccttccagt aactccgggg tatagctgct cccgccacca
    14221 atagtgacga cttttaattt ctggctcata atttctccct tcagtacaga atactgatat
    14281 ctggcatatc tgcccccccg gacataaata atccagcaac aggacagata tgtgaattgt
    14341 caggtataac gacttactga ttaaattcag tcaatttctt acgatatctc ttaggcgtaa
    14401 aggacgttaa tttcttaaac gttttaataa acaaactcgg gctactataa ccggcctcaa
    14461 acgcaatatc cgtcacggaa tagttggtca tttccagttg tttttttgca aaattaatac
    14521 ggatctcatt aataatctgc attggtgttt tgccataata tcgttgagtc gctcgcgtca
    14581 aatattcctg tgatttggct gacaacgtca ccatattctc cagcgccgat tcactaaact
    14641 gctctttatc atgcatcttt tctaccgtac ttttcagcca ctacggtaca tcatcaatca
    14701 cctgttcttc gcggtaatga cgtaaacggt taatgacata aaaagtaacc atctcaacaa
    14761 actcttccag ccctgtttcg cggaaattta atgaagaaat cactgtttcc acataggtga
    14821 gaaaagcgtt attggtccgg tatacctgcg aagcaacaaa gcaataaggc aacaacggca
    14881 ggtaatgctg ctcaaaaaag cgtttactga tcccaacgtt caatatgcgc gtggcaccaa
    14941 actcataaaa actttgatgg tgcgaaccta acggaataaa aacaaaatcg ccccgttcca
    15001 gtaacacgcg cttaccgtta atctcctaga aataacgccc ggttaatact agagtaaatt
    15061 cataatagtc gtgctgatgc agtccgctga tactctcagt tttgttatag ataaacacat
    15121 ggaagttttt gccattaaac aactgctgtt ctcgggcagt ggcaatttcc ggcgcgttaa
    15181 tcactggctg catcatcgac tccttatgcc ttcagttttt catgaagctc aattaattca
    15241 gtaatcagtt cacgcgcaag catggacgtc attaaatgat cctgagcgtg gaccagcacc
    15301 agactcactt tcatcttacc ttcgcccgca tcgccttcaa tcagtttcgt ctgtaccaga
    15361 tgcgcttcat tcaatgccat ccgtgactga tccatcatgg ctttcgctgc ggcaaaatcg
    15421 ccctgcttcg cctgtttcaa tgcggcatac gccaggctac gcgcttgtcc agagttgatg
    15481 ataagcccca tcaccacttc ttccagctct tcagcttctg tttgcgtatc aggaatatta
    15541 tcgagatcca tcatacatcg ttcctctctc ttcttaccgg caagattacc cgtaccggca
    15601 tcgattaaaa tttcaggggt tagcgatatc ttcttcgctc tcttctttat caatcgcatt
    15661 ttgtgctttg ttagccacca caacaaaggg cagataaatt aacgttgcga tgccaaggtt
    15721 gaagagtgcg accagcaatg cggcgacgct accgttggtg ttaaagaagg ctcccagacc
    15781 ggttggcatg gtccacggtg caatattggt caccggagga atgatgccca tgtagtacgc
    15841 tgcgagggtg attgccgcca gaatcggttg taccagtaca aacgggataa acatcaccgg
    15901 gttcatgata attggcagac caaacagaat cggttcgtta atctggaaga tgccggacgg
    15961 cagcgccagt tttgccacct gacgataatc agcacggcga gaggcgataa agatagccag
    16021 gatcaggcct aaagtcgcac cactgccccc aaggaaaata aaggagtcca gcatcggctt
    16081 ggcccagata tggaaggtct taccggctgc cagcgccgct tcgacggaac catattgctg
    16141 ataggtcgcg atattttcca gcgcccacgg cgtcataatg ccgttgtcca gtgcggtcag
    16201 cgccagcgcg ccatgaatac cgaagaacca gagcagtgga acaaagatca cataggccca
    16261 gcccaccacg ctacccaacg atgccagtgg ggttgagatg gtatccataa tgatctgatg
    16321 gaagttggtg ccccaggtat tcaacgccca ggcaataatc cccatcacgg aaagaataat
    16381 aaagccggga attaatgccg agaaagaacg cgatactgaa gcaggtacgc tgtcgggcag
    16441 tttaatgacc caattgcggc ggacaataaa ggtaaacatt tctgccacca ccaagccaat
    16501 aataatcccg gagatgatat tcgccccacc taaccagttt gcgccaaccg cataggcctc
    16561 accgacacta tatggggtga cggtcataaa tgctgcaacg gataacaacc cagccgccag
    16621 cgcatcgact ttacgctctt ctgccagcgc catgccaata aagaacggtg ccattaaaga
    16681 cattattcct aatgttccgt tatatacgtt gccgccaata cctttcagac cattaagtgt
    16741 ttcaatggtt gaggcgtcga ggcgaatacc taaggaataa aaaaacgacc cctccccaaa
    16801 gcttagaaaa acgttgttaa ttaatacaaa catggccccc gcaagggtta acggcattaa
    16861 gcgaataaag ccatttttga ttgcattaac gtgtggctgc tttcctattt taactgcaaa
    16921 agggaggagt accttttcaa gcgatgcaat aacattactc atagaaaaat acccttaaaa
    16981 accgcaattt aaatattacg gtattgattt atgaaataac tctttgacgg gaaaatttaa
    17041 aataaattaa tttgctgcgg cttttttaat cgctgcaacc gcagccttaa gcacgcctaa
    17101 accatcgact ttgccataaa gcagcgagtc aattacttca accggtttgt tgggtaacaa
    17161 acgctggatt tcgggcaaca tataagcaat ctgcggccct aataacacga catcggcatt
    17221 ctgacctttt tcaccagcca gtgtttccgg aaatgcttca ataatgaccg gaacttcata
    17281 tttttctgcc tgtgcgcgca tttttgatac cagtaaagag atagacatgc ccgcagaaca
    17341 aaacagataa atgtgtttct tttccataaa actgccctca tcgacgatta tctgtcagcc
    17401 agacactccg caagccttga cctgcttcca tgctctgggt aacttgcgaa accaacatga
    17461 ctttttttgt ggctgaaagg agtatacggt atagacctga gcaatagtat ttctttgacc
    17521 ttataaattg tttctccttg acctgtggtt atgaccctct tcacatttcg ggcaaataat
    17581 tcgcgaagat aattaagaac tcaggccaaa aaaaaccggc gcaatggccg gtttccgttg
    17641 ttactcaagc tttcagacga attgattact tcgcagcctg tggatcagtg tcgtattcag
    17701 cacaggtctg ataaccggag ttgatgacat gtccggtatc atctaacgcg acaaagtagg
    17761 tttctgcttt accatcacgt tgacccagga tgtaggtctg gcaagtaccg cgagcatgga
    17821 tcatgctcac ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc cggctcatgc
    17881 cttttttgac gtctttcacc acaggctgta caaactggtc tttggtacga tcataagctg
    17941 tacaacccgc cagcatggtt aataccgccg ctgcactcag aattcctgcc atattcttgt
    18001 tcatattccg tcctcttgtt tatcagcgtg ttagataagc ctggaataca ttgggcgctt
    18061 tttcaagccc gtgaacgaaa cggctccgct ttcagaggat tcctgtatga cgttttaacc
    18121 accattcagc ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt aaggaagttt
    18181 tgtcttttct gtctggaggg gttcaatgac attgcaacaa caaataataa aggcgctggg
    18241 cgcaaaaccg cagattaatg ctgaagagga aattcgtcgt agtgtcgatt ttctgaaaag
    18301 ctacctgcaa acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga
    18361 ctccacgctt gccggaaagc tgtgccagat ggcgattaat gagctgcgcc aggaaactcg
    18421 caacgaatca ctgcaattta ttgccgtacg cctgccctat ggtgttcagg ctgacgaaca
    18481 agattgccag gatgccattg cctttattca accggatcgc gtattaaccg ttaatatcaa
    18541 gggcgcggta ttggctagcg agcaggcatt gcgggaagca ggcattgaac tgagcgattt
    18601 tgtccgtggc aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat
    18661 gaccagcggt gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg gattcttcac
    18721 taaatatggt gacggcggta cggatattaa tccgctgtat cgtctcaaca aacgccaggg
    18781 taaacagtta ctgacggcat taggttgccc ggaacacctt tataagaaag ccccaactgc
    18841 cgatcttgaa gatgatcgcc cttctctgcc agatgaagtg gcactcggcg tgacctatga
    18901 caatatcgac gactatctgg aagggaaaaa cctacctgaa caggtcgcca gaacaataga
    18961 gaactggtat ctgaaaaccg aacataaacg ccgtccgcca attaacgttt tcgatgattt
    19021 ctggaaaaag taataatttg cacctattgg attgtgcgaa aaagagtaat ttgttcacgc
    19081 cggatgcggc gtgaacgcct tattcgacct acaaaactat gcaaattcaa tacattgcag
    19141 gagtcgaata ggcctgacag gcgtagcacg tcagacggtg taacctttgt catcaacccg
    19201 cctctttttt aatcgcctcc cgcctgttac actggataga taaccagcat tcggagtcaa
    19261 cagtggtacg gcgtttaact tctccgcggc tcgaatttga agctgcggca atttatgaat
    19321 atcccgaaca tttacattca ttccttaatg acttacccac ccgaccaggg gtgtatctgt
    19381 ttcatggcga aagtgacacc atgccgctct atatcggcaa aagcgttaat atccgcagtc
    19441 gggtgctttc tcatttacgc accccggatg aagccgccat gctacggcaa tcccggcgaa
    19501 ttagctggat ctgcaccgca ggcgaaatcg gcgctctgct ccttgaagcg cgattaatca
    19561 aagaacaaca gccgctgttt aataaacggt tgcgccgcaa tcgccagctc tgtgccctgc
    19621 aattaaatga aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt tcacgagccc
    19681 ccaacctgtt tggcctgttt gccaataggc gcgcagcttt gcaagcattg cagagcatcg
    19741 ctgatgaaca aaaactttgt tatggcctgt tgggactgga accgttaagt cgcggtcgtg
    19801 catgttttcg ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa gagagccatg
    19861 aggaacatgc gctacgcttg cgccagtctc tggagcgttt gcgagtggtg tgttggcctt
    19921 ggcaaggtgc ggtggcactg aaagaacagc acccggaaat gactcaatat catattattc
    19981 aaaactggct gtggctgggg gcggttaatt cgctggaaga agcgacaacg ttaattcgga
    20041 cacccgccgg gtttgatcac gacggttata aaattctttg taagccgctg ctttccggta
    20101 actatgaaat tactgaactt gatccggcga atgaccagcg agccagttga tttcactgaa
    20161 caacagttta ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga accagcacgc
    20221 gccctggtcg gtagtgagca gtttgtcgcc cagttgccac gccccgttaa tcacaaacac
    20281 caccccgccg cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga ctttcgcctt
    20341 acagacatcc aggcgcgtca taatattgaa atccatcgac atctgccctg ccgtcagttt
    20401 cgcttttacc acctggtccg ccgtaaaggc aaaaggctgt aacggtttta aggtatggtt
    20461 aaagcggtct gcgctttcaa ggagcatctc gccgccttcc agcaacgtca ctatcctttc
    20521 catgccggga aacaaagaaa actccccatt cgccgcgatg gatgcaatgc tggcacgcca
    20581 gtaaaaatca cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac cggcagcgtt
    20641 tcgccacaga ttcaccgaca ttttacgcat atcaaagtat tccatactcg ctcccggccc
    20701 gttcttttca ttgttatcgt tcaggtaacc gtggatggtt atcggcaact cgggtgattt
    20761 acgttagtgg tgatcaggct ttttttacaa ctgtttgaga agagaaaaga aaaccgccga
    20821 tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa gttttttggc
    20881 tgaaagatta ttcagcagtt gcaggcattt taccttttgc cgctggacgt tctgtcagac
    20941 gcttctcaaa attagcatta aattgctttt tctgttccgg cgtcaggatg ttgtaaattt
    21001 tgttctgggt ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc atttttgcga
    21061 tctgcgcttc agcttttgct ttatcgaagg tatcgctggc aatgatgtca tgcattgcgc
    21121 ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg gcctttcatg atttcgtgga
    21181 tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc tgatgcggac
    21241 cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc cggtgctgcg gtagtggtgt
    21301 ctgcggcatg ggccaggtta gccgcgccaa gagccagggt agaggcaaca aacagtgcag
    21361 ttaatttacg catattctat atccttcctt tcagttattt attacggctt tcttaagtag
    21421 cgtgccgtgt tgacgagatt aactttactg actttagcgt caattaatca gagcaacggt
    21481 aaaacaatga aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa agaaaatttg
    21541 atggagagtg atgagagaat attacaacac gatgattttg cagagattat gaagaactat
    21601 accggatgac tggtgataaa taaagcaaat aaccaggatt aatctgtatt aatttataag
    21661 aaagcaactt aatacccgca gaatgatttc tgcgggtaag tattagctta ttttttcgag
    21721 cattaatccc gcgcgtaatc ccaacgctac caacggatta gggaataaca catactctac
    21781 atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc agcaatgttc ctttctcaaa
    21841 cggcataaaa ttcagcgtgt cacttgccat atgcatttcg aaggacggcg agtggcgagt
    21901 aatttgcgaa accacccgat aacggagcgg cggtgttctc acgataccga cactctcacc
    21961 agatagcagc gcagcaattg cgctggcagt tactgcaaac tggcgaagat cgttttgccc
    22021 aaagggcaac gctttgccaa gttccaacgt acaggccagc gcgccaaaat gtctgacgct
    22081 gaaatgggta aacgtaccac caggctcctg atggaacacc agcgcctcca gccccgccgc
    22141 acccagccac gtcagaaatt tctcgtccca gggaatgtcg cgttgcggta atacaccgaa
    22201 ctgcggatgc aaggagccac gaattgcggt atgtagatca aggtgccaac gcacagattc
    22261 tttgccctgg tcataaaaat cttccaggca ctgttccagt tcgcgcgccc gacaggtttc
    22321 tccgctttca gcaaatagct gccaacgacc gccaaacatt cggttcatat cgctatggca
    22381 ataacgtttc ccttgcttca gcgcaggagg attcccgagg atcaccagca accgccaacg
    22441 taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg tccagcattt ccacaggtgc
    22501 cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg ccttgcggtg gcgttaatgg
    22561 cgtcagttcc agcacaccat cgcccagcca gcgccagcga acgccgttga tttctcgctc
    22621 ggtgataacc ggttttttac ccgttaaggt cagagcaaga aaattatcca ttgccccctc
    22681 cctcgcgctg gaacggataa accgaaccga gattcagtaa ttggctcaat acatccagtg
    22741 cttcccgccc ttcgcgcagc aattgcgggt cggccagatc ggcagcagta agacgatcgc
    22801 ggtagtaacg atccacccag tcattgagcg cattaaacaa ggtatcgttc atcatcaccg
    22861 ccggattcac cgcccggcgt tcttcttctg tcaataccac ccgcaaccgc aggcacgccg
    22921 ggccgccgcc attcgccatg ctttcacgta agtcaaagac ttttagttcg ctaatcgggt
    22981 tgtcagcggc aaggagttca ttgagataac cccatactcc ggcgtgttcc cgacactcct
    23041 gaggcagcac caacatcatg gaaccatcat cgcggctcaa cagttggctg ttaaacagat
    23101 aggtagacac cgcatccgac acggaaacct gagttgccgg aacttctatc gccataaaac
    23161 cgttcacccg cgtgccagta actgtgactg gcgagcgaac gcctgttggt ggcaaaacag
    23221 cacctggcgg ttactcacgg caatcacgtc attatgaaaa acgctctggt cgataacgtc
    23281 cgggttttgc tgggcgaaaa tcacctgttg gggattcacc tgattaagcc ttgccaccac
    23341 ctcgctggct tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat cattgccttc
    23401 ttctcgcccg tagacaaaaa gtagcatacc cggttcaccg taatgaccgc cgagacgatt
    23461 gtggtttgcc gccccctcat caccgagcaa cgctacctgc ggcaacgccg aatggacgct
    23521 aaatttctct tcgtccttaa aaatcgcttt taacagcgat tcagtgacgg gcgcttccag
    23581 cgaacggtga aatttattgt tcaggttggc aacggtgaga tgcactttgc catccagcgt
    23641 atcggcagat ggcgcgatcg ttgccgcatt ggctacccac attggcgaag cggaactgac
    23701 gctggaaagc cagtgcggtg cctggcgtgc aactttttcc agtacctgct catcgctacc
    23761 actgaatccc aactgacgca gcaccggaat aaacggacgc tcgtgcggcg ggatcacggc
    23821 ctgggggaat cccgcatcgg caagggcttt cattttcagt aagccctgtt ttgccgccag
    23881 tcgcgggtta gacacctgaa aacggtgacg ggtagaggct tcattaccaa acgacaggcc
    23941 cgcgtaatga tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg cgttcatcgc
    24001 accacctcat cggaaaaatc cagcccgggg ttaagcgtgg cgggcaatgt taacgagtcc
    24061 gactccaggc tcgccatagg ccatgcgcag taatctgcgg cataccaggc gctggggcga
    24121 tggttaccgg aagcaccaat gccgccgaat ggggcggtac tggcagcacc agtaagcggt
    24181 ttgttccagt taacaatccc cgcccgcgcc tccagcaaca gttgatcgaa tttttcccgc
    24241 tcgggggaaa ccagaccgca aaagagtccg aagcgagtgt tgttcgccat ccgaatcgcc
    24301 tcatcaaaag tatcataacg ccagacgcgc aaaagcggcc cgaacacctc ttcatccggc
    24361 acaccgccaa cgcccgtcat ttcaatgatc cctggtgtca gcaacgatgt ccctgcttgt
    24421 aataagcgcg gcgcaagcag ggttcgtccg cccatcgctt ccagttgctg ccaggcagta
    24481 accacctgct gtgcggcctg ttcagaaatc agcccgccaa taaacggctg cggttcgtca
    24541 tcccagttgc ccggcgttaa tcgctggctg acggcaacca gactagcaag aaacgcatcg
    24601 ccctgcgctc cgcttttgag caataaacgg cgggcgcagg tgcagcgttg cccggctgtg
    24661 acaaacgccg actgaatggt cagatggaca gccgcgtcga tatccgccac ctcatcgata
    24721 attagcggat tattaccgcc catctcaagg gcgagaattt tctccggctg accggagagc
    24781 tggcgatgca gctggtagcc ggtattggcg ctaccggtaa acagcaaacc gtcgagatcc
    24841 tccagcgcac tcagcgcctg acccgtttca cgcccgccct gcaccaggtt cagcacgccc
    24901 ggcggcaagc cagcctgctg ccataaacgc attaccgctt cgccactcca cggtgtcagt
    24961 tcgctgggtt taaagataat ggtgttacct gccagcaatg ccggaacgat atgtccgttc
    25021 ggcaaatgac cagggaaatt atacggccca aacaccgcca gcacgccgtg cgggcgatgt
    25081 cgcaggctcg ccgcaccgtc cggcatttca ctacgctgct cgccggtacg aacgtgatac
    25141 gccttaattg atatcgcgat tttattgatc atcgccgtca cttcggttgc cgcttcccag
    25201 cgcggcttac ccgtttctct ggcaataatc gcggttaatt cggctttatt gctttccagc
    25261 aatccggcaa agcgttcgac aacgacctga cgttcagcca atgagagccg cgcccagcgc
    25321 ggaaacgccg cacgggctgc ccgacaagcc tgttcgacct gagcggcatc ggcatcattg
    25381 ccttgccata acacctcgcc cgataccgga ttacgcttca cacgcgatgc gccctggccc
    25441 gttatccagt caccgttaat ccataaagtc atgctgtttt ctcctctgcg cacaggcgca
    25501 ccagacgaac gcgatcccct gcgtggcatt tgagggcatc cagttgtgcg gcggttaaaa
    25561 tcaaacgctc ggttgccgga tcggtacgcg ccagcaccac gcggaaatgg tgataatttt
    25621 cattggcgac caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct gccacttcca
    25681 ccagccgact tttacggatg gcgcgcacgc ggtcgatgtc acactcaagc gttggcccgc
    25741 cgtcgaagat gtcgatatag ttgcggtaac gaaaaccttc tttctccagc accgcgcggg
    25801 caggcgcggt ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg gataaaaagt
    25861 gggtatagat cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc ccggtgccgc
    25921 agagaaaatc ggcgcggcta aaatccatcg aaaagaagcg tttaccgagg ctttgccaga
    25981 acggtgaata gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact ttgtcattaa
    26041 acttgtcgcg aaaagccgcc ataaacataa agcgcgattt cgacagcaaa tagccgttgc
    26101 cctctttgcg ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg ccggtgtgat
    26161 cgttactaag aaacagcgtc ggcaatgcgt tatagacatt cagctctttt gaggcgtgaa
    26221 ccaacgtgcc gacgcgatag ttgtaccagg gatcgttcag cccaaccgcc acctcaatag
    26281 cgcaaatccc cgccacggtg cctgtctcgc tatcttccag cacgaacaca tagccctgct
    26341 cgcttttggg cagttcacct tgccaggttt tgattgccct ttcgatacgc gccgaaagcg
    26401 tggcttcatt ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca agctgcatca
    26461 gcgccgagac atctgagcgc tcaacgggac ggatgaccat catgatgaac ctcggctaac
    26521 aaagtgttcg caagcagctg caaagcgatc cagtccggtc gtcacctctt cttcgctgac
    26581 attgagcgca ggcgcaaaac gcaccacgtt gccacccgca atcagtacca tcacgcctgc
    26641 tttcgccgct tccagagaga tctgtttcgc ttgcccggcg taatcggcat tcagtacaca
    26701 gccaatcaac aaacctaagc cgcgaacttc actgaacaaa ccatagcggt gattaatagt
    26761 attaagacgc tcaacaaacc agtcgtgacg ctgtttaacg ccattaagca tctctggtgt
    26821 gttgatgagc tccagcactt tgcctgctac cgccgaggcc agcggattac cgccataggt
    26881 ggtgccatga gtgccaacgg tcatcacgcg ggcgcactct tcggttgcca acaacgcacc
    26941 gaccgggaaa ccgccgccca gcgctttggc ggtggttaac agatcgggcg tcacgccgta
    27001 gtgcatatag gcatacagct caccagtgcg cccgacgccg gtttgtacct catcaaaaat
    27061 cagcaaggca ttgtggcggt cacacaattc acgcagacct tgtaaaaacg cgttgctggc
    27121 tggcaccaca ccgccttcgc cctagatggg ctcgacaatc accgcacagg tggcgtcgtc
    27181 aatcagcgcg ctggcagagt taatatcgtt atatgcagca tgacgaatat ccgccggcag
    27241 tggcgcaaaa tcctgtgaat aggctggctg cccacccgca ctgacagtaa acagcgtgcg
    27301 accatgaaac gcatttttga acgccacgat gccactctta tggctgccgt agcggtcgtg
    27361 agcgaattta cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg agttacaaaa
    27421 gaagacgcga tcggcaaacg tggcgtcgat caattttttc gccagtcgca gtaccggctc
    27481 gttggtgtaa ccgttgccgg tatgccagaa cttactcgcc tgttcgttca gcgcttcacg
    27541 cagttccgga tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga agtcgatata
    27601 ctctttcccc tgctgatccc acaagcgcga accttcgcca cgtaccggta taaagggtgc
    27661 cggagcgtaa acaggtatca tccattcatc aaagttttca cgcgtaattg gctgagacat
    27721 agcgacctct acagtaaata attcgttatt tgtatgttaa caataagtaa tgtttgcgct
    27781 gtaaatgtag attgcagggt tcgtgccagc cagtgataaa agtgcataaa cggcggaggc
    27841 taactggaaa tcaaggagtt ataaccaaac catatgcatt taaagtgcat ataaagtgaa
    27901 tacgtttgcg atgtgggtga ataaaaagaa taaaaaacgc aatgttatgc agaagtaaaa
    27961 tataattcta gaattgtgat cattgacgaa atttactgga aattactgcg ccattctgac
    28021 gcagcgcgca ccaaaagcgg gcattttttg cgccatcgtt gacatcatta acaaccatcg
    28081 atcaaatcac ttaacaacag gcggtaagca acgcgaaatt ctgctaccat ccacgcactc
    28141 tttatctgaa taaatggcag cgactatgaa atttgtctct tttaatatca acggcctgcg
    28201 cgccagacct caccagcttg aagccatcgt cgaaaagcac caaccggatg tgattggcct
    28261 gcaggagaca aaagttcatg acgatatgtt tccgctcgaa gaggtggcga agctcggcta
    28321 caacgtgttt tatcacgggc agaaaggcca ttatggcgtg gcgctgctga ccaaagagac
    28381 gccgattgcc gtgcgtcgcg gctttcccgg tgacgacgaa gaggcgcagc ggcggattat
    28441 tatggcggaa atcccctcac tgctgggtaa tgtcaccgtg atcaacggtt acttcccgca
    28501 gggtgaaagc cgcgaccatc cgataaaatt cccggcaaaa gcgcagtttt atcagaatct
    28561 gcaaaactac ctggaaaccg aactcaaacg tgataatccg gtactgatta tgggcgatat
    28621 gaatatcagc cctacagatc tggatatcgg cattggcgaa gaaaaccgta agcgctggct
    28681 gcgtaccggt aaatgctctt tcctgccgga agagcgcgaa tggatggaca ggctgatgag
    28741 ctggggggtg gtcgatacct tccgccatgc taatccgcaa acagcagatc gtttctcatg
    28801 gtttgattac cgctcaaaag gttttgacga taaccgtggt ctgcgcatcg acctgctgct
    28861 cgccagccaa ccgctggcag aatgttgcgt agaaaccggc atcgactatg aaattcgcag
    28921 catggaaaaa ccgtccgatc acgcccccgt ctgggcgacc ttccgccgct aatttagcag
    28981 ctctcctggc tcaaactggg tcaggagaat taaccttgag aaaaatcaac aaactgtcag
    29041 taatgatttg ttgcctgccg tcctttgtta taccgtcact gcgtttttag ttgtctgacc
    29101 acttctctat tatcaagttt gatataggaa actccacgat gaacgctgag cgtaactttc
    29161 tgtttgcctg tcttattttt gcgctggtca tttacgctat ccacgctttc ggtttattcg
    29221 atctgctcac cgatttaccc cacttacaga cactcattcg ccagagcgga ttgttcggtt
    29281 atagcctcta tattctgtta ttcatcaatg ccccctcttt ctgttaccag gaagcatact
    29341 ggtgatcgct ggaggaatag tttttggccc gctcttaggg acacaactct cattaattgc
    29401 cgcaacgctg gcctcctcgt gctcattcct gctggcgcgc tggctgggac gtgatttact
    29461 gctgaaatac gtgggccata gccatacctt ccaggccatt gaaaaaggca ttgcgcgtaa
    29521 cggtattgat tttcttattc tgacccgctt aatcccgttg tttccttaca atattcaaaa
    29581 ttacgcttac ggattcacca ccatcgcctt ctggccttat acccttattt cggcactcac
    29641 gaccctgccc ggtattgtta tttataccgt gatggcaagc gatctcgcca atgaaggcat
    29701 tacgctgcgc tttattttac aactctgtct ggcgggcctg gcgctgttta ttctcatcca
    29761 gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg tctgcttcgc gccgcagccc
    29821 acttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca gtgtcatgga
    29881 aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc tgcgatcaaa
    29941 aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa ccgctgcgcg
    30001 tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat taatggtcgt ttcctcaccg
    30061 acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa tcgctgttta
    30121 tggcttatgc gcccccgaaa gcattttatg aagccctgaa agaggcaggt ggtacgccgg
    30181 gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc aaactggata
    30241 tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg attgttgaca
    30301 gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca gaagagaaga
    30361 aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc aatgcaacat
    30421 acacttatgg tgcggtggaa aaacacggcg aagtgaaatt caaaggcaat gcctcagttc
    30481 tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa agccaagata
    30541 aaggatgatg aagatacaat cgcgtaaaat ctggtactac cgtataaccc tcatcatcct
    30601 gttgttcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt ttatcaatcg
    30661 cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta ttcagtctta
    30721 cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca ttgctgcacc
    30781 gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt tctggggcgg
    30841 cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta tcgccagagt
    30901 gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca gtatggacgg
    30961 ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc cttttgtccc
    31021 tttcgatcca atcagctatg ctgccggtct gacttcaata cgttttcgct cgttttttat
    31081 cgccaccggg cttggtcaat taccggcgac tattgtttat tcctgggcag gcagcatgtt
    31141 aacaggcggt actttctggt ttgtcaccga actgtttatt ctgtttgccc tgaccgtggt
    31201 gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgact gatgggttta
    31261 ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca tcgtcgccat
    31321 tacggc
//
LOCUS       DAJVPP010000044        30243 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVPP010000044 DAJVPP010000000
VERSION     DAJVPP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.044"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            68..1726
                     /locus_tag="OQM34_001960"
     CDS             68..1726
                     /locus_tag="OQM34_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1452421.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1723..2679)
                     /locus_tag="OQM34_001961"
     CDS             complement(1723..2679)
                     /locus_tag="OQM34_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCS1452422.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2830..3945
                     /gene="macA"
                     /locus_tag="OQM34_001962"
     CDS             2830..3945
                     /gene="macA"
                     /locus_tag="OQM34_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1452423.1"
                     /translation="MKKRKTVKKRYVIALVIVIAELITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3942..5888
                     /gene="macB"
                     /locus_tag="OQM34_001963"
     CDS             3942..5888
                     /gene="macB"
                     /locus_tag="OQM34_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1452424.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5961..6185)
                     /gene="cspD"
                     /locus_tag="OQM34_001964"
     CDS             complement(5961..6185)
                     /gene="cspD"
                     /locus_tag="OQM34_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1452425.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6508..6828
                     /gene="clpS"
                     /locus_tag="OQM34_001965"
     CDS             6508..6828
                     /gene="clpS"
                     /locus_tag="OQM34_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1452426.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6859..9135
                     /gene="clpA"
                     /locus_tag="OQM34_001966"
     CDS             6859..9135
                     /gene="clpA"
                     /locus_tag="OQM34_001966"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1452427.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQITPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9478..9565)
                     /locus_tag="OQM34_001967"
     tRNA            complement(9478..9565)
                     /locus_tag="OQM34_001967"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9529..9531),aa:Ser,seq:gga)
     gene            complement(9819..10037)
                     /gene="infA"
                     /locus_tag="OQM34_001968"
     CDS             complement(9819..10037)
                     /gene="infA"
                     /locus_tag="OQM34_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1452428.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10322..11026)
                     /gene="aat"
                     /locus_tag="OQM34_001969"
     CDS             complement(10322..11026)
                     /gene="aat"
                     /locus_tag="OQM34_001969"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1452429.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11068..12789)
                     /gene="cydC"
                     /locus_tag="OQM34_001970"
     CDS             complement(11068..12789)
                     /gene="cydC"
                     /locus_tag="OQM34_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1452430.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12790..14556)
                     /gene="cydD"
                     /locus_tag="OQM34_001971"
     CDS             complement(12790..14556)
                     /gene="cydD"
                     /locus_tag="OQM34_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1452431.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14679..15644)
                     /gene="trxB"
                     /locus_tag="OQM34_001972"
     CDS             complement(14679..15644)
                     /gene="trxB"
                     /locus_tag="OQM34_001972"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1452432.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16188..16682
                     /gene="lrp"
                     /locus_tag="OQM34_001973"
     CDS             16188..16682
                     /gene="lrp"
                     /locus_tag="OQM34_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1452433.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16817..20845
                     /gene="ftsK"
                     /locus_tag="OQM34_001974"
     CDS             16817..20845
                     /gene="ftsK"
                     /locus_tag="OQM34_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1452434.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21000..21611
                     /gene="lolA"
                     /locus_tag="OQM34_001975"
     CDS             21000..21611
                     /gene="lolA"
                     /locus_tag="OQM34_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1452435.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21622..22965
                     /gene="rarA"
                     /locus_tag="OQM34_001976"
                     /gene_synonym="mgsA"
     CDS             21622..22965
                     /gene="rarA"
                     /locus_tag="OQM34_001976"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1452436.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23056..24348
                     /gene="serS"
                     /locus_tag="OQM34_001977"
     CDS             23056..24348
                     /gene="serS"
                     /locus_tag="OQM34_001977"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1452437.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24588..27032
                     /gene="dmsA"
                     /locus_tag="OQM34_001978"
                     /pseudo
     CDS             24588..27032
                     /gene="dmsA"
                     /locus_tag="OQM34_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27043..27660
                     /gene="dmsB"
                     /locus_tag="OQM34_001979"
     CDS             27043..27660
                     /gene="dmsB"
                     /locus_tag="OQM34_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1452438.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27662..28453
                     /locus_tag="OQM34_001980"
     CDS             27662..28453
                     /locus_tag="OQM34_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1452439.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28489..29115)
                     /locus_tag="OQM34_001981"
     CDS             complement(28489..29115)
                     /locus_tag="OQM34_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1452440.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29430..>30243
                     /locus_tag="OQM34_001982"
     CDS             29430..>30243
                     /locus_tag="OQM34_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1452441.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQ"
BASE COUNT         7454 a         7455 c         8167 g         7167 t
ORIGIN      
        1 gtattagcaa ggcctttccc gttatactgc gagcgtaaag gataagtcac atatttctgg
       61 aggggatatg attcttgagc gcgttgaaat tgtgggtttt cgcggtatca accgtttgtc
      121 gttgatgctc gaacaaaaca acgtcctgat tggggagaac gcgtggggta aatccagctt
      181 gctggacgcc ttaaccctgc tgctatcgcc agaatcagat ctctaccatt ttgagcgcga
      241 cgatttctgg ttcccgccgg gagatatcaa cgggcgagaa catcatctgc atattatttt
      301 gaccttccgc gaatcgctgc caggccgaca tcgggttcgc cgttatcggc cgctggaagc
      361 gtgctggacg ccatgcaccg atggctatca ccgtattttt tatcgtctgg aaggggagag
      421 tgcggaagac ggcagcgtga tgacactgcg cagttttctc gataaagacg gacatccgat
      481 tgatgttgag gatattaacg atcaggcacg ccatctggtg cgtttaatgc cggtgctgcg
      541 cttgcgtgat gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa atgtccccaa
      601 tgtggaagtc accgcgcgcc agctcgattt cctcgcccgt gagttgtcct cacatccgca
      661 aaatctctct gatggacaga ttcgtcaggg actttccgca atggtgcagt tgcttgagca
      721 ttatttctct gagcaggggg ctggacaggc gcgatatcgt ttaatgcggc ggcgggccag
      781 caatgagcaa cgaagctggc gctatctgga catcatcaac cggatgattg accgacctgg
      841 agggcgctcg tatcgggtta ttttgctcgg cctgtttgct actttattgc aggcaaaagg
      901 cacattgcga ctggataaag acgcgcgtcc gttattgcta attgaagatc cagaaacccg
      961 cctgcatccg attatgcttt ccgttgcctg gcatctgttg aatcttttgc ctttacagcg
     1021 tattgctacc accaactctg gcgagctgct ttcgttaact ccagtggaac atgtttgccg
     1081 cctggtgcgt gaatcttctc gtgttgccgc ctggcgtctg gggccaagtg gcttgagcac
     1141 cgaagacagc cgccgcatct cttttcacat tcgttttaat cgtccgtcat cgctgtttgc
     1201 tcgctgctgg ttgctggtgg aaggggaaac ggaaacctgg gttatcaatg aactggcgcg
     1261 tcagtgcgga catcattttg atgccgaagg gatcaaggtc attgagtttg cccagtccgg
     1321 gctaaagcca ctggttaaat ttgcccgccg aatggggatt gaatggcatg tactggtcga
     1381 tggcgatgaa gcagggaaga aatatgccgc tacggtacgc agcctgttga ataacgatcg
     1441 ggaagccgaa cgagaacatt taacggcgtt accggcgctg gatatggaac attttatgta
     1501 tcgccaggga ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa atgtaccgat
     1561 gaatctacgc aaaattatct cgaaagcgat ccatcgctct tccaaacccg atcttgccat
     1621 tgaagtggca atggaggcag gacgtcgtgg tgtggactcc gtaccaacgc tgctgaaaaa
     1681 aatgttctca cgcgtgctgt ggctggcgcg cggtcgcgcg gattaaccgc gaaacatcgt
     1741 ggccatttgt ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt cagcccgttt
     1801 tttactggcg atttcggcaa tctcttttcg tgctatctgt gctggaaggc ggtaatggcg
     1861 ttcagcatca catacgccgc caaccgactc ccagaacgcg ttgtaatcag catggatctt
     1921 gccttcttta tcgcgataac gcaggctgcg gtaaatatgc gtttcattgc tgacggcaat
     1981 aatctgctct acctgcaaac gttgggcaaa cagacaggcc gcttccatca cgaggcgttt
     2041 gggaaatagc ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg gaatttccca
     2101 ttttgcgcct tgcagtccgc caataaacat cgttcttttc ccctgatatt cacacagggt
     2161 aaacgtgatc tctgccagag gaataccttc gctgttgcgg aacaggattg tgctgtcacc
     2221 ttctttatcc attgagatca tcatggtcag ctcaagcgtg aactgctcac cgtttttgcc
     2281 ttccagttta gccagttgca gcccgggggt attcaaatat aagctgaatt cttccgccga
     2341 catacacccg cggagtaacg cataatggta acgtaacgcc tccagcaatt gcttacggct
     2401 aagattcgcg gcaaggtaag ggcgatgcag acgcacaggc agtcgcggct ggcgcgttaa
     2461 caacacattg agattaggcc agtgggaaag ttcgttcatc cactcaacgc ttaaacgcgg
     2521 cataatcaac gagcgcagca aaaatttctg gcgaaaacta cagcgatgcc agaatttacc
     2581 cggccgacac tgtccacgtg ccagactaag aaaaagtgac aggctgctga gagattcaga
     2641 tggcgtaaag gtccgttcag ttagctgcga catattcatg aaatcaatgg ttatacatga
     2701 cgtcgatttc accattgcgt atcttaacca aacatcaata gtgtgattac taacgtaaat
     2761 tttagggttt tgttgatatt tcgttgaagt taatgacccg gattggcata tggagtattc
     2821 agaatattta tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc gctggtgata
     2881 gtcatcgccg aactgattac gttatggaga attcttaacg cacccgtgcc gacttatcag
     2941 acactgattg tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg aaagctggac
     3001 gcgctgcgta aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac gctgtcggtg
     3061 gcgattggcg ataaagtaaa aaaagaccag cttttagggg tcattgatcc tgaacaggct
     3121 gaaaaccaga tcaaggaggt cgaagcaacg ctgatggagc tacgcgcgca gcggcagcag
     3181 gcggaagcgg agctgaaact ggcgcgggtg acgtattccc gtcagcaacg tctggcacaa
     3241 acgcaagcgg tttcactgca ggatctcgat accgccgcga cggagatggc tgtgaaacag
     3301 gcgcaaattg gcaccattga cgcgcaaatc aagcgcaatc aggcctctct cgatacggct
     3361 aaaaccaatc tcgattacac ccgtatcgtt gccccgatgg ctggggaagt gacgcaaatc
     3421 accactctgc aaggccagac ggtgattgcc gcacagcaag caccgaacat tctgacgctg
     3481 gcagatatga gcactatgct ggtaaaagcg caggtttctg aagcggatgt aatccacctg
     3541 aagccggggc aaaaagcctg gtttacggtg cttggcgatc cactgacgcg ctacgagggg
     3601 caaatcaagg atgtgctacc gacgccggaa aaggtaaacg acgctatttt ctattacgcc
     3661 cgttttgaag tacccaaccc caatggtttg ctgcggctgg atatgaccgc gcaagtgcat
     3721 attcagctca ccgatgtgaa aaatgtgttg acgatccctc tgtcggcgtt aggcgatccg
     3781 gttggcgata atcgttataa agtcaaattg ttgcgtaatg gtgaaacacg cgagcgtgaa
     3841 gtgacgattg gcgcacgtaa cgataccgat gttgagattg tcaaagggct ggaagcgggc
     3901 gatgaagtgg tgattggtga ggccaaacca ggagctgcac aatgacgcct ttgctcgaat
     3961 taaaggatat tcgtcgcagc tatcctgccg gtgatgagca ggttgaggtg ctgaagggca
     4021 tcagcctcaa tatttatgcg ggtgagatgg tcgcgattgt tggcgcttcg ggttccggta
     4081 aatcgaccct gatgaatatt ctcggctgcc tggataaggc caccagcggc acctatcgcg
     4141 tagccggtca ggatattgcc acgctggacg ccgatgcgct ggcgcaactg cgccgcgagc
     4201 atttcggctt tattttccag cgttaccatt tgcttccgca tttaaccgcc gagcagaacg
     4261 ttgaagtacc cgccgtctat gccggtcttg agcggaaaca gcgactgctt cgtgcccagg
     4321 agttgctgca acggctgggg ctggaagacc gtacagagta ttatccggca cagctttcgg
     4381 gtggtcagca acagcgcgtc agcatcgcgc gggcattgat gaacggcggt caggtaattc
     4441 ttgccgatga accgaccggc gcactggaca gccattctgg cgaagaggtg atggcgatcc
     4501 tgcatcagct gcgcgatcgc gggcatacgg tgattatcgt cacccacgat ccgcaggtcg
     4561 ctgctcaggc cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc aatcctcccg
     4621 ccattgaaaa agtgaatgtt gctggcggga cggagcctgt tgtcaataca gtgtctggct
     4681 ggcggcagtt tgtcagcggt tttaacgagg cgctgacgat ggcatggcgg gcgctggcag
     4741 cgaataaaat gcgtacttta ctgaccatgc tggggattat tatcggtatt gcgtcggtgg
     4801 tttccattgt cgtggtgggt gacgccgcca aacaaatggt gctggcggat attcgttcta
     4861 ttggtacgaa tactattgat gtctatcccg ggaaagattt tggcgatgac gatccgcaat
     4921 atcagcaggc gctgaagtac gacgacttaa tcgccatcca aaaacaaccg tgggtcgcct
     4981 cagccacacc tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt gatgttgctg
     5041 ccagtgccaa tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc ttcagtgaag
     5101 gaaacacctt taatcaggag cagctgaacg gtcgtgcgca ggtcgtggtt ctcgacagta
     5161 atactcgccg ccagcttttc ccccataaag cagatgtggt tggcgaggtg attctggtcg
     5221 gcaatatgcc cgccagagtc attggtgtgg cggaagaaaa acagtcgatg tttggtagca
     5281 gtaaagtgct gcgtgtctgg ctaccttaca gcacgatgtc cgggcgagtt atgggccagt
     5341 cgtggcttaa ctccattact gtcagggtga aagaaggatt tgacagcgcc gaggcggaac
     5401 agcaactcac gcgtttactt tcactgcgcc acggtaagaa ggatttcttt acctggaaca
     5461 tggacggcgt cttgaaaact gttgaaaaga ccacacgtac tttacaactg tttctgacgc
     5521 tggtggcggt gatttcgctg gtggtgggcg gtattggtgt aatgaatatt atgctggtgt
     5581 cagtgaccga gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg cgagcaagcg
     5641 atgttttgca acagttcctg atcgaagccg tactggtttg cctggtcggt ggcgcgttgg
     5701 gaataacact gtcactgtta attgctttca ccttgcagct tttcttaccc ggctgggaga
     5761 ttggtttttc accgttggcg ctgctgctgg cgtttctctg ctcgacggcc accgggattt
     5821 tatttggctg gttacccgca cgaaatgcgg cacgactgga tccagtagat gctctggcac
     5881 gggagtaatt tttgagataa aaatgccagc cgatcgggct ggcattttgc ctttaggatg
     5941 tacacaatga gacagaagag ctatgcgact gccgcttcta cttcgacggg cacaataaca
     6001 ctggcgtgat tgccttttgg cccctggtgg acatcaaact gaacggattg tccagctttt
     6061 agcgttctgt aaccatccat ctgaatggtg gaataatgag cgaaaatatc ttcgccgccg
     6121 ccttcagggc agatgaaacc aaaccctttg gcattgttga accacttaac agtacccttt
     6181 tccatgcttc gacatccttc gcaaatctta tacaagtaag atggaataaa ccggggtcag
     6241 agagggggct gttcaaaacc tcgccaactc tagaaataca atttagagaa ttagggcgag
     6301 ccgtcaagca tttgacaggg gccagggggc aggtatgaat caaaaatttg aagcagttaa
     6361 cgctattgac aggaatgtga cagatgtcgc tgatgccaac gatagatgat agttatctat
     6421 catgtggagt agattggtca ggcaaataag ctcttgtcag cggcagggcg ttctgccgat
     6481 aaccgtaacc gaagatgata actgacaatg ggtaaaacga acgactggct ggactttgat
     6541 caactggcgg aagaaaaagt tcgcgacgcg ctaaaaccgc catctatgta taaagtgata
     6601 ttagtcaatg atgattacac tccgatggag tttgttattg acgtgttaca aaaattcttt
     6661 tcttatgatg tagaacgtgc aacgcaattg atgctcgctg ttcactacca ggggaaggcc
     6721 atttgcggag tctttaccgc cgaggttgca gaaaccaaag tggcgatggt gaacaagtac
     6781 gcgagggaga atgagcatcc attgctgtgt acgctagaaa aagcctgaat gcaggcataa
     6841 aaattggggg aggtgcctat gctcaatcaa gaactggaac tcagtttaaa tatggctttc
     6901 gccagagcgc gcgagcaccg tcatgagttt atgaccgtcg agcacttgtt actggcgctg
     6961 ctcagtaacc catctgcccg ggaggcgctg gaagcgtgtt ctgtggattt ggttgcgctc
     7021 cgtcaggaac tggaagcctt tattgaacaa atcacacccg ttctgcctgc cagtgaagag
     7081 gagcgcgaca cacagccgac gctgagtttt cagcgtgtac tgcaacgtgc ggtcttccat
     7141 gtccagtcct ccggtcgcaa tgaggttacc ggtgcaaacg ttctggtcgc tatcttcagc
     7201 gaacaggagt cgcaggcggc atatctgttg cgtaaacatg aagtcagccg tctcgatgtg
     7261 gtgaacttta tctctcatgg cacgcgtaaa gacgagccga cacagtcttc tgatcctggc
     7321 agccagccaa acagcgaaga acaagctggt ggggaggaac gtatggagaa tttcacgacg
     7381 aacctgaacc agcttgcgcg tgtgggcgga atcgacccac taattggtcg tgagaaagag
     7441 ctggagcgtg ctattcaggt tctctgccgt cgccgtaaaa acaacccgct gctggtgggg
     7501 gaatctggtg tcggtaaaac cgcgattgca gaaggtcttg cctggcgaat tgttcagggt
     7561 gatgtgccgg aagtgatggc tgactgtacg atttactctc tcgatatcgg ttctctgtta
     7621 gcgggcacaa aatatcgcgg cgactttgaa aaacgtttta aagcgttgct caagcagctg
     7681 gagcaggaca ctaacagcat cctgtttatt gatgagatcc acaccattat cggtgcgggt
     7741 gcagcgtctg gtggtcaggt cgatgcggct aacctaatca aaccgttgct ctccagcggt
     7801 aaaattcgcg taattggttc gacaacctat caggagttca gcaacatttt cgagaaagac
     7861 cgtgctctgg cgcgtcgctt ccagaaaatt gatattactg aaccgtcgat cgaagaaact
     7921 gttcaaatca tcaatggcct gaaaccgaag tatgaagcgc accacgacgt gcgttatacc
     7981 gcaaaagcgg tgcgtgcggc ggtagagctg gcggtgaaat acattaacga tcgtcatctg
     8041 ccggataaag ccattgatgt tatcgacgaa gcgggcgctc gcgcacgtct gatgccggta
     8101 agcaaacgca agaaaaccgt taatgtggcg gatattgagt ccgtggtggc ccgtattgca
     8161 cgcattccag agaagagtgt ttctcagagt gaccgcgata ccctgaaaaa actcagcgat
     8221 cgcctgaaaa tgctggtctt cggccaggat aaagccattg aggcgctgac tgaagccatt
     8281 aagatggcgc gtgcaggttt aggtcacgaa cataaaccgg tcggttcgtt cctgtttgcc
     8341 ggtcctaccg gggtcgggaa aacagaggtg acggtacagc tttcgaaagc gttgggcatt
     8401 gagcttctgc gctttgatat gtccgagtat atggaacgcc ataccgtcag ccgtctgatt
     8461 ggtgcgcctc cgggatacgt tggttttgat cagggcggtt tgctgaccga tgcggtaatc
     8521 aagcatccac atgcggtgct gctgctggac gaaatcgaga aagcgcatcc ggacgtgttc
     8581 aatattctgt tgcaggtgat ggacaacggt acgctgaccg ataacaacgg acgcaaagcg
     8641 gacttccgta acgtggtgct ggtgatgacc actaacgccg gggtacggga aactgagcgc
     8701 aaatccattg gtcttatcca ccaggataac agcaccgatg cgatggaaga gatcaagaag
     8761 atctttacac cggaattccg taaccgtctc gacaacatta tctggttcga tcatctgtca
     8821 actgacgtga tccatcaggt ggtggataaa ttcatcgtcg agttgcaggt tcagctggat
     8881 cagaaaggtg tttctctgga agtgagccag gaagcgcgta actggctggc cgagaaaggt
     8941 tacgaccggg caatgggcgc acgtccgatg gcgcgtgtca tccaggacaa cctgaaaaaa
     9001 ccgctcgcca acgaactgct gtttggttcg ctggtggacg gcggtcaggt gacggttgcg
     9061 ctggataaag agaaaaatga gctgacttac ggattccaga gtgcacaaaa gcacaaggcg
     9121 gaagcagcgc attaatctga ttgtcaggta ggttggtgaa gtccgtaatc tcgtcagggg
     9181 ttacggactt tttatttatg gggtggaacg gtgaaaaccc tatttttgga ggtgaaggta
     9241 agttgttgat aattagtgct gctggaaggt aaggataaaa aagggtgctg caggagaatg
     9301 ggatggtttt gctttattaa caacgggcta aacgtgtagt atttgagttc actgccgtac
     9361 aggcagctta gaaattcaca ggtaacatac tccacccgcc caccatgttc actgccgtac
     9421 agacagataa aatgcgaaaa aaaagctcgc actttcgtac gagctcttct ttaaatatgg
     9481 cggtgagggg gggattcgaa cccccgatac gttgccgtat acacactttc caggcgtgct
     9541 ccttcagcca ctcggacacc tcaccaaatt gttttgctac caaacctcat gggtggcaac
     9601 ggggcgctac tatagggagt tggagtaaaa cggtcaagaa gaattttaat gataattatt
     9661 gtttgctcat actgtaaaca acttgtgcag tatatctaca tcgagacaag ttatggactt
     9721 atacttccaa agtacttcat acatatcaca aaataaaaag gccggttaaa ccgacctttt
     9781 actcgttctt tctcttcgcc catcaggcgg taaaacaatc agcgactacg gaagacaatg
     9841 cggcctttgc tcaggtcgta cggggtcagt tcaacagtca ctttgtcgcc cgtcaggatg
     9901 cggatgtagt ttttgcgcat tttaccggag atgtgtgcag taaccacgtg accgttttct
     9961 aactctacgc ggaacatggt attaggcaac gtttcaagaa cggtaccttg catttcaata
    10021 ttgtcttctt tggccatcta atcctctggg gtatcactac cgtaatttga accggcaaga
    10081 taatgccgaa gttctgtaaa taagtaaaga tttgcgcgct aaatcgcaac aaacaggttc
    10141 ggcacattac tccgaaaaca cacggctaag ccgcaccaaa agcgcaacgt ataagggagc
    10201 ggtgagataa acgatgggcg ttacctgacg cgaaaaattc cttatcggca gcggggtaat
    10261 gagcgtaacc aactctgcga ccgcaattat aacactctgg ggagaaatgt gccgaaaaca
    10321 ttcattcttg tggtgaaaac aagcaccgtg gtacccagaa attattcggc aatcgtccga
    10381 ggcgcatttg attgagataa ttaaggtaat cccggcgggg aatttcgcag gcaccaagcg
    10441 atgctgtgtg atcgttaagg acctggcagt cgataagctt accgccatga ccgataaatt
    10501 cctcacagaa taccagaagc gccgttttag acgcattttc catccggctg aacatggact
    10561 cgccacaaaa tagcgttccc tgggccacgc cgtacatacc gccgacaagc tcatcttcac
    10621 gccagacttc aatggagtgg gcatgaccga gttcgtgaag gcgatggtag gcttcgacca
    10681 cgccacgcgt gatccaggtt ccttcttcgc gatcgctggc acagccttca atgacctgac
    10741 caaaagcgta attcatcgtg acacgatagg gcgagcgttt atgaaatcgc ttcatactac
    10801 ggctgatatg cagtgattct ggccatagca ccgcgcgggg atcgggcgac caccagagga
    10861 tggggtcgcc tggagaaaac cacggaaaaa taccacgctg gtaagccatt aacaggcgcg
    10921 cagggctaag atcgccccca agtgccagca ggccgttagg ctcacgtaat gcgccttccg
    10981 gggaagggaa ggctattgaa tggcgagaaa gctgaaccaa gcgcatgacc gcaaaactcc
    11041 acgcaagtcg gatcgttcaa taatagctta caaaccctgc ttgaactggt aataacgccc
    11101 ctgtctggca agcagttctg cgtgagtacc ttgctcaata atttgcccgt tgtccatcac
    11161 tattatttgt tggaaacgag agagtccgcg aagtcgatgg gtgaccatta acaccgtttt
    11221 ctcacgcatc atttttgcaa gcaattcaag gatctggctt tcggttgtgg catctaagcc
    11281 ttcggtaggt tcatccagca acaccagtgg cgcatcatgt aacagcgtac gggcgatagc
    11341 cagacggcgc agttcaccac cggagagctg gcgtccgcct tcacctaacc aactgttgag
    11401 acctgcatcc tcgagcagct tctccagacc aacgcgacgc aagatctccg acagagcctc
    11461 atcactactg ccaggcgagg cgagtaacag attatcacgc agcgtggcgc taaacagatg
    11521 cactcgctga ggaacaacgc tgatggtctg tcgtagagcc gcttcattca ggctggctat
    11581 ggggctatcg ttaagcaaaa tctcgccctg ttgcgggtcc catgcgcggg tcagcagttg
    11641 taacagtgtt gatttgccgc atccggttcg cccgagaatc gctatatgtt ccccggcgtt
    11701 tacctgaaga gaaatccctt taagtgcctg ttgggattgc tccggataag tgaactgaac
    11761 atcccgtaat gtcagcgaaa cgcgatcggc aacacgagtt tgggtatcag ggaaggtgac
    11821 ctccggtttt tgatccgtta agtcagagat acgtacggca gaggcaatga cttgccccag
    11881 atgctgaaat gcacccgtta ctggtgccag tgcttcaaac gcggctaacg cacaaaagac
    11941 aaacagggca attaatgcgc cgggttgagc attgccgcca acgccgccag acgccatcca
    12001 cagcatcagg atcaccgcta acgcgccaat gagcagcatt atcgcttgcg acaatgcggt
    12061 cagttcagat tgacggcgtt gcgcttccag ccattgaatt tctgtattct ctagttgcgt
    12121 gcgataacga tcgctggcac caaaaatggt cagctcagct tgcccttgca gccaggccgt
    12181 cagttgttgg cgatactgtc cgcgaagatg agtcagattt tgcccggtgc tttttcccgc
    12241 acgataaaac agcggtggca tcaggaaaag cgtcagtaac ataacgctgc ccagcgtaaa
    12301 ggcgagggtg aaatcaagga aacttaaccc gattgtcacc accataatca ccacaaaagc
    12361 gcccaccagc ggcgagataa cgcgcaggta aagatgatcg agcgtatcaa catccgccac
    12421 cacgcggttg agcaattcgc cctgacgata gcgcgccagt ccggcagggg agaggggcaa
    12481 caatttgctg aaggtgtaaa tgcgcagatg ctgcaacacg cggaaagtcg cgtcgtgact
    12541 taccagacgt tcaaaataac gcccggcagt acgggtgatt gcggcaccac gcacgcccgc
    12601 agcgggtagc atatagttga agctgtacag tccggcaacc cccgcaaccg ctgaggccga
    12661 gaggaaccag ccggaaagtg tcaacagacc gatactggcg agcagcgtca caattgccag
    12721 cacaatacca agacttaaca tccatttatg acgtttatac agtgccagat agggtagcaa
    12781 agcgcgcatt taaatctcct cctgacgatg ggccagtaat gtggcgaatg ggccaccagc
    12841 cacacttaat tccgcgtaac gtccttgctc aataatccgg ccatcctgca taacccaaat
    12901 gacatcccag tcagcaagat cttctaactg gtgggtgacc attaacgttg tctggcgcag
    12961 agaggcggca ttcagcgcct ccattacgcg ctgttcactg tgagcatcaa ggctggcagc
    13021 gggttcatcc aacagtaata gcgaacaggg atttagtaac gcacgggcca ccgccacgcg
    13081 ctgcgcctgc cccacggaaa ggcgggcagc ctggtcgcca acaggtgtat caacgccttg
    13141 tggcaggagc ggtagaaact cgctgaccca ggcgttatcc agcgctgctt gtaattcttg
    13201 ttcgctggca tcaggtcgcg ccagtagtac gttatcccgc aattttgctg ccggtaattg
    13261 tgggttttgc ccaacccagg agagatgttt acgccatgat tctggtgata aatcacgtaa
    13321 ttctatcccg ttgattcgta atgatccctg atatgagaga aaaccagaaa gcgcgttcag
    13381 cagtgagctt ttacctgaac cgctgcggcc aaccaacact gcacgttggc ctgctggcaa
    13441 agtaaagttc agcggtccgg ccagcgtttt accttccggc gacgtgataa acagatcctc
    13501 ggcctcaatg gtcagcggat cggtcaatgc taattccgcc tcaccgcgct gcggatgggc
    13561 gagcggggtt tccataaacg ttttcagact gtcagctgca ccaacagcct gggctttagc
    13621 atgataaaac gtaccgagat cgcgtaatgg ctggaaaaac tctggcgcaa ggatcagggc
    13681 cagaaaacct gcagccagcg tcaccccagt atcgtagtga ccaaaatcca gctcgccgag
    13741 ataggaaaaa ccaaagtaga ccgccaccag agcaattgac agcgaggtaa aaaattcgag
    13801 aatgccggag gataaaaacg ccagtcgtag cacttccatt gtccgttggc ggaaatcttc
    13861 cgaagcagaa cgaatacttt caatttcagc ttcaccacga ccaaaaatac gcaatgtttc
    13921 catgccgcgc aggcgatcga ggaaatgccc acttaagcga gcaagagcga gaaagttacg
    13981 ccggttagca tcggcagccc ccattccaac cagcgccata aacaacggaa ttagcggtgc
    14041 tgtgcccagc agaatgagcg ccgcagccca gttagagggg aagatcgcca ccacaatcag
    14101 caacggcacc gacactgcca gcgccatttg cggcagatag cgtgcatagt aatcatgcat
    14161 atcgtcaatt tgctcgagta ccagcgtcgc ccagctcccc gcaggtttac cctgaatcca
    14221 cgctggccct gcttgttgca gacggtcgag aacctgacgg cggatggcaa agcggatatg
    14281 ctgcccggcg tgataaccca cccgttcgcg taaccagacc acccatgcgc gcaatacaaa
    14341 ggtcagaacc agtaacgtaa agggaagcag cagggcttca cggggaatat tctccataat
    14401 catatgttgc agaattcgcg ccatgaacca ggcctgggca atgatcaata tgccgcttac
    14461 aaagcccagc agacgagaaa tattcagcca acgttgggag atgacgcttt gctgttttaa
    14521 ccagcgggtt aactcttttt gacgagattt attcattgca cgcttagcag gtgagttatc
    14581 agaattattt gcagagcaat gttacaacgg ggaaaaaata aaggcgaccc atagtcgcaa
    14641 ggtgtcgcct tctttacttt tgttactgat ttgtaaaatt attttgcgtc agctaaacca
    14701 tcgaggtagc gttccgcatc aagtgctgcc atgcagccag taccggccga agtaatggcc
    14761 tggcgataaa tgtgatccat cacgtcgcct gcggcaaaga cgccaggaat gctggtctgg
    14821 gtggcattac catgaatacc cgactgtact ttgatgtagc cgttttccag ttccagctgc
    14881 ccttcgaaaa tcgcagtatt cgggctgtga ccgatagcaa caaacagacc ggcaacgtcg
    14941 agtgactcga tgttatcgct gttttgcgta tcgcgcagac gaacgccagt gacacccatt
    15001 tgatcgccgg tcacttcttc cagcgtacgg ttggtgtgca gaatgatgtt gccgttctcc
    15061 actttatcca tcagacgctt aataaggatt ttttccgcgc ggaaaccgtc acggcggtga
    15121 atcagatgca cttccgaagc gatgttagac agatacagcg cctcttcaac cgcggtattg
    15181 ccgccgccga tgaccgcaac tttctggttg cgatagaaga aaccgtcgca ggttgcacaa
    15241 gcagaaaccc cacggccttt aaaggcttct tcagagggca ggccgagata gcgtgcagaa
    15301 gctccggtgg caataatcag cgcgtcgcaa gtgtattcgc cgttatcgcc attcagacgg
    15361 aacggacggt tttgcagatc caccttgttg atatgatcaa aaatgatctc agtttcaaac
    15421 ttggtggcat gttcgtgcat gcgcgccatt aataacggac cggtcagatc gtttggatca
    15481 ccaggccagt tttccacttc cgtggtggtg gtcagttggc cgcctttttc catgccggta
    15541 atcagcacag gttgcaggtt ggcgcgcgcc gcgtagacag cagcggtgta tcccgccggg
    15601 cctgaaccca ggataagcag tttactgtgt ttggtcgtgc ccatgagatc cccatagttg
    15661 ttggcagaca atgggcagga ttgtagggaa tttacagacg taaaaaaaga gtatgacgat
    15721 tttgttaaca atttgtgcaa tcggcagcat cgataagcag gtcaaattct cccgtcatta
    15781 tcacctctgc tacttaaatt tcccgcttta taagccgatt aaatgatgaa taaacgcccc
    15841 tgttaatgaa tatctggcat gttgtactaa aaatcgatgt tttgctttga caatcccctg
    15901 gtgttttgcg aaaacattcg aggaagaaaa aaacagtatt cttatatgcg cataaccatg
    15961 tatgtaaata ccatgtttac cgtgctagtg aaatctacgt atggcgtgga cagacgccat
    16021 tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca ccgtagaccc aggcattgcg
    16081 cgccgtgaat cttcatgatt tcggtctatc gtgacgggta gcgactctga acagtgatgt
    16141 ttcagggtca gacaggagta gggaaggaat acagagagac aataataatg gtagatagca
    16201 agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa cattcttaat gagttgcaaa
    16261 aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt gggactttcc ccaacgccgt
    16321 gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat tcagggctat acggcgctgc
    16381 ttaaccccca ttatctggat gcatcacttc tggtattcgt tgagattact ctgaatcgtg
    16441 gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca aaaacttgaa gaaattcagg
    16501 agtgtcattt agtatctggt gatttcgact acctgttgaa aacacgcgtg ccggatatgt
    16561 cagcttaccg taagttgctg ggggaaaccc tgctgcgtct gcctggcgtc aatgacacac
    16621 ggacatacgt tgttatggaa gaagtcaagc agagtaatcg tctggttatt aagacgcgct
    16681 aacacggaac aggtgcaaaa tcggcgtatt ttgattacac tcctgttaat ccatacagca
    16741 acagtactgg ggtaacctgg tactgttgtc cgttttagca tcgggcagga aaagcctgta
    16801 acctggagag cctttcttga gccaggaata cactgaagac aaagaagtca cattgacaaa
    16861 gttaagtagc ggccgccgcc ttctggaggc attgctgatc cttattgtcc tgtttgccgt
    16921 ctggttgatg gctgccttac taagctttaa cccttcggac cccagctggt cgcaaacggc
    16981 ctggcatgaa cctatccata atttaggtgg gatgcccggt gcgtggttgg cagatacgct
    17041 gttctttatt tttggcgtga tggcttacac cattcccgtc attattgtcg gcggttgttg
    17101 gtttgcctgg cgtcatcagt ccagcgacga atacattgat tattttgccg tttcgctacg
    17161 catcattggc gttttggcgc tcatccttac ctcctgtggt ctggcggcaa tcaacgctga
    17221 cgatatctgg tattttgcct ccggtggcgt cattggcagc ttactaagca ctacgctaca
    17281 accactgcta cacagtagcg ggggaactat tgcgctgctc tgcgtttggg cagcgggcct
    17341 gacgttgttc accggttggt catgggtgac cattgctgaa aaactcggcg gctggatttt
    17401 aaacattctc accttcgcca gtaatcgtac ccgtcgcgat gatacctggg tcgatgaaga
    17461 tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac ggcaaacagc atgaatcacg
    17521 ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa cggttggcgg aaaaattcat
    17581 taatccgatg gggcggcaaa cagacgctgc gttgttctcc ggtaagcgga tggatgatga
    17641 cgaagagatt acctacactg cacgcggtgt ggctgccgac ccggacgacg tcctattttc
    17701 gggcaatcgt gcaacgcagc cagaatatga cgaatacgat ccattattaa acggtgcgcc
    17761 aattaccgaa cctgtcgctg tagcagctgc tgctaccacg gcgacacaaa gctgggctgc
    17821 gccggttgaa cctgtgactc agacgccgcc tgttgcctct gttgatgttc caccttcgca
    17881 acctacagta gcctggcagc ctgtaccggg tccacaaacg ggagagccgg ttattgctcc
    17941 tgcaccggaa ggttacccac agcagtcaca atatgcgcag cctgcagtgc aatataatga
    18001 gccgctgcaa caaccagtac agccgcagca gccgtattat gcacctgcag ctgaacaacc
    18061 tgcgcaacag ccgtattatg cccctgcgcc agaacaaccg gtggcaggta acgcctggca
    18121 agccgaagag cagcaatcca cttttgctcc acagtctaca taccagactg agcaaactta
    18181 tcagcagcca gccgctcagg agccgttgta ccaacagccg caacccgttg aacagcagcc
    18241 tgttgtggag cctgaacccg ttgtagaaga gacaaaaccc gcgcgtccgc cgctttacta
    18301 ttttgaagaa gtggaagaga agcgagcccg tgaacgtgaa caacttgcgg cctggtatca
    18361 accgattcca gaaccggtta aagaaccaga accgatcaaa tcttcgctga aagcaccttc
    18421 tgttgcagca gtacctccag tagaagccgc tgccgctgtt tctccgctgg catctggcgt
    18481 gaaaaaagcg acactggcga cgggggctgc cgcaaccgtt gccgcgccag tcttcagtct
    18541 ggcaaatagc ggtggaccgc gtccgcaggt caaagagggg attggcccgc agttgccacg
    18601 accgaaacgt atccgcgtgc caactcgtcg tgaattggcg tcttacggta ttaagctgcc
    18661 ctcacagcgt gcggcggaag aaaaagcccg tgaagcccag cgcaatcagt atgattctgg
    18721 cgatcagtat aacgatgatg aaatcgatgc gatgcagcag gatgaactgg cacgtcagtt
    18781 cgcccagaca cagcagcaac gctatggcga acagtatcaa catgatgtgc ccgtaaacgc
    18841 agaagatgca gatgctgcgg cagaggctga actggctcgt cagtttgcgc aaactcaaca
    18901 acaacgttat tccggcgaac aaccggctgg ggcgaatccg ttctcgctgg atgattttga
    18961 attttcgcca atgaaagcgt tgctggatga tggtccacac gaaccgctgt ttacgccaat
    19021 tgttgaacct gtacagcagc cgcaacaacc ggttgcacca cagcagcagt atcagcagcc
    19081 acaacagccg gttgcgccac agccgcagta tcagcagcca caacagcagg ttgcgccgca
    19141 gccacaatat cagcagccgc aacaaccggt tgcgccacag cagcaatatc agcagccgca
    19201 acaaccagtt gcgccgcagc cgcagtatca acagccgcaa caaccggttg caccgcagcc
    19261 gcagtatcag cagccacaac agccagttgc gccacagccg caggataccc tgcttcatcc
    19321 gctgttgatg cgtaatggcg acagccgtcc gttgcacaaa ccgacgacgc cgctgccttc
    19381 tctggatttg ctgacaccgc cgccgagcga agtggagccg gtagatacct ttgcgcttga
    19441 acaaatggcg cgtctggtgg aagcgcgtct ggctgatttc cgtattaaag ccgatgtcgt
    19501 taattactct ccggggccag ttatcactcg ctttgaactt aacctggctc ctggcgtaaa
    19561 agcggcgcgc atttctaact tgtcacggga ccttgcccgt tcactttcga cggtggcggt
    19621 gcgtgtcgtt gaagttattc ctggcaaacc ctatgtaggt ctggagttac caaataaaaa
    19681 acgacaaacc gtttatctgc gcgaagtttt ggataacgcc aaattccgcg ataatccgtc
    19741 gccattaacc gtggtgctgg gtaaagatat cgccggtgag ccggtggttg ccgatctggc
    19801 gaaaatgccg cacttgttgg ttgcggggac taccggttcc ggtaaatctg tcggtgtgaa
    19861 cgcgatgatc ctgagcatgc tttataaagc acagccagaa gatgtgcgtt tcatcatgat
    19921 cgacccgaaa atgctggagc tttcggttta tgaaggcatt ccgcacctgt taacggaagt
    19981 cgttactgat atgaaagatg ccgccaacgc gctgcgctgg tgtgttaacg agatggagcg
    20041 tcggtataaa ctgatgtctg cgctgggtgt gcgtaatctg gcgggttata acgaaaaaat
    20101 tgctgaagcc gatcgcatga tgcgtccgat tccagacccg tactggaagc cgggtgacag
    20161 tatggatgcc cagcatccgg tgctgaaaaa agaaccatac attgtcgtgt tggttgacga
    20221 atttgccgac ctgatgatga cggtaggtaa aaaagtggaa gagctgatag ctcgtctggc
    20281 gcaaaaagcc cgtgccgcgg gtatccacct cgtactggca actcagcgtc catcggttga
    20341 tgttattact ggtctgatta aagcgaatat tccgacccgt atcgccttta ccgtatccag
    20401 taagattgac tcacgtacca ttcttgatca ggctggcgcg gaatcactgc tgggtatggg
    20461 ggatatgctc tactctgggc cgaactccac gttgccggta cgtgtccatg gtgcttttgt
    20521 tcgcgatcag gaagttcatg ccgtggtgca ggactggaaa gcgcgtggtc gcccacagta
    20581 tgttgatggc atcacctccg acagcgaaag cgaaggtggt gcgggtggtt tcgatggcgc
    20641 tgaagaactg gatccgttgt tcgatcaggc ggtgcagttt gtcactgaaa aacgcaaagc
    20701 gtcaatttct ggcgtacagc gtcagttccg cattggttat aaccgtgcag cgcgtattat
    20761 cgaacagatg gaagcgcagg ggattgtcag cgaacagggg cacaacggta atcgtgaagt
    20821 gctggcccca ccgccgtttg actaattaat gcattgccgg ataaggcgcg gtagcgtcgc
    20881 atccggcact ctatcaactg aaaattcagt attttcttct ttcctcaagc tgattattag
    20941 cctggaatag agagtagagg gaactcccga tcgggagtga cgtaatttga ggaataatga
    21001 tgaaaaaaat tgctatcacc tgtgcattac tctcaagctt agtagcaagc agcgtttggg
    21061 ctgatgccgc aagcgatctg aaaagccgcc tggataaagt cagcagcttc cacgccagct
    21121 tcacacaaaa agtgactgac ggtagcggcg cggcggtgca ggaaggtcag ggcgatctgt
    21181 gggtgaaacg tccaaactta ttcaactggc atatgacaca acctgatgaa agcattctgg
    21241 tttctgacgg taaaacactg tggttctata acccgttcgt tgagcaagct acggcaacct
    21301 ggctgaaaga tgccaccggt aatacgccgt ttatgctgat tgcccgcaac cagtccagcg
    21361 actggcagca gtacaatatc aaacagaatg gcgatgactt tgtcctgacg ccgaaagcca
    21421 gcaatggcaa tctgaagcag ttcaccatta acgtgggacg tgatggcaca atccatcagt
    21481 ttagcgcggt ggagcaggac gatcagcgca gcagttatca gctgaaatcc cagcaaaatg
    21541 gggctgtgga tgcagcgaaa tttaccttca ccccgccgca aggcgtcacg gtagatgatc
    21601 aacgtaagta gaggcacctg agtgagcaat ctgtcgctcg atttttcgga taatactttt
    21661 caacctctgg ccgcgcgtat gcggccagaa aatttagcac agtatatcgg tcagcaacat
    21721 ttgctggctg cggggaagcc gttgccgcgc gctatcgaag ccgggcattt gcattctatg
    21781 atcctctggg ggccaccggg taccggcaaa acaaccctcg ctgaagtgat tgcccgctat
    21841 gcgaacgctg atgtggaacg tatttctgcc gtcacctctg gcgtgaaaga gattcgcgag
    21901 gcgatcgagc gcgcccggca aaaccgcaat gcaggtcgcc gcactattct ttttgttgac
    21961 gaagttcacc gtttcaacaa aagccagcag gatgcatttc tgccacatat tgaagacggc
    22021 accatcactt ttattggcgc aaccactgaa aacccgtcgt ttgagcttaa ttcggcactg
    22081 ctttcccgtg cccgtgttta tctgttgaaa tccctgagta cagaggatat tgagcaagta
    22141 ctaactcagg cgatggaaga caaaacccgt ggctatggtg gtcaggatat tgttctgcca
    22201 gatgatacac gacgtgccat tgcagaactg gtgaatggcg acgcgcgccg ggcgttaaat
    22261 acgctggaaa tgatggcgga tatggccgaa gtctatgata gcggtaagcg ggtcctgaag
    22321 cctgaattac tgaccgaaat cgccggtgaa cgtagcgccc gctttgataa caaaggcgat
    22381 cgcttttacg atctaatttc cgcactgcat aagtcggtac gtggtagcgc acccgatgcg
    22441 gcgctgtact ggtatgcgcg aattattacc gctggtggcg atccgttata tgtcgcgcgt
    22501 cgctgtctgg cgattgcgtc tgaagacgtc ggtaatgccg atccacgggc gatgcaggtg
    22561 gcaattgcgg cctgggattg ctttactcgc gttggcccgg cggaaggtga acgcgccatt
    22621 gctcaggcga ttgtttacct ggcctgcgcg ccaaaaagca acgctgtcta cactgcgttt
    22681 aaagccgcgc tggccgatgc tcgcgaacgc ccggattatg acgtgccggt tcatttgcgt
    22741 aatgcgccga cgaaattaat gaaggaaatg ggctacgggc aggaatatcg ttacgctcat
    22801 gatgaagcaa acgcttatgc tgccggtgag gtttacttcc cgccggaaat agcacaaaca
    22861 cgctattatt tcccgacaaa caggggcctt gaaggcaaga ttggtgaaaa gctcgcctgg
    22921 ctggctgaac aggatcaaaa tagccccata aaacgctacc gttaatgtta tcgttgcggt
    22981 aatgttgtta ctgtatccct gtggtcgcag gctgtggcca catctcccat ttaattcgat
    23041 aagcacagga taagcatgct cgatcccaat ctgctgcgta atgagccaga cgcagtcgct
    23101 gaaaaactgg cacgccgggg ctttaagctg gatgtagata agctgggcgc tcttgaagag
    23161 cgtcgtaaag tattgcaggt caaaacggaa aacctgcaag cggagcgtaa ctcccgatcg
    23221 aaatccattg gccaggcgaa agcgcgcggg gaagatatcg agcctttacg tctggaagtg
    23281 aacaaactgg gcgaagagct ggatgcagca aaagccgagc tggatgcttt acaggctgaa
    23341 attcgcgata tcgcgctgac catccctaac ctgcctgcag atgaagtgcc ggtaggtaaa
    23401 gacgaaaatg acaacgttga agtcagccgc tggggtaccc cgcgtgagtt tgactttgaa
    23461 gttcgtgacc acgtgacgct gggtgaaatg cactctggcc tcgactttgc agctgcagtt
    23521 aagctgactg gttcccgctt tgtggtaatg aaagggcaga ttgctcgcat gcaccgcgca
    23581 ctgtcgcagt ttatgctgga tctgcatacc gaacagcatg gctacagtga gaactatgtt
    23641 ccgtacctgg ttaaccagga cacgctgtac ggtacgggtc agctgccgaa atttgctggc
    23701 gatctgttcc atactcgtcc gctggaagaa gaagcagaca ccagtaacta tgcgctgatc
    23761 ccaacggcag aagttccgct gactaacctg gtgcgcggtg aaatcatcga tgaagatgat
    23821 ctgccaatta agatgaccgc ccacacccca tgcttccgtt ctgaagccgg ttcatatggt
    23881 cgtgacaccc gtggtctgat ccgtatgcac cagttcgaca aagttgaaat ggtgcagatc
    23941 gtgcgcccag aagactcaat ggcggcgctg gaagagatga ctggtcatgc agaaaaagtc
    24001 ctgcagttgc tgggcctgcc gtaccgtaaa atcatccttt gcactggcga catgggcttt
    24061 ggcgcttgca aaacttacga cctggaagta tggatcccgg cacagaacac ctaccgtgag
    24121 atctcttcct gctccaacgt ttgggatttc caggcacgtc gtatgcaggc acgttgccgc
    24181 agcaagtcgg acaagaaaac ccgtctggtt cataccctga acggttctgg tctggctgtt
    24241 ggtcgtacgc tggttgcagt aatggaaaac tatcagcagg ctgatggtcg tattgaagta
    24301 ccagaagttc tgcgtccgta tatgaacgga ctggaatata ttggctaata cccaattttt
    24361 ctgaatctaa aaagcgcctt tagggcgctt tttttgtctc cctttgatac cgaacaataa
    24421 ttactcctca cttacacgta atactacttt cgagtgaaaa tctacctatc tctttgattt
    24481 tcaaattatt ctatgtatac aagcctatat agcgagctgc tatagaaata attacacaat
    24541 acggtttgtt actggaatca atcgtgatca agcttgagtg agccattatg aaaacgaaaa
    24601 tccctgatgc ggtattggct gctgaggaga gtcgccgtgg tttggtaaaa acgacagcga
    24661 tcggcggcct ggcaatggcc agcagcgcat taacattacc ttttagtcgg attgcgcacg
    24721 ctgtcgatag cgccattcca acaaaatcag acgaaaaggt tatctggagc gcctgtacag
    24781 ttaactgtgg tagtcgctgc ccgctacgta tgcacgtcgt ggacggtgaa atcaaatatg
    24841 tcgaaacgga caataccggc gatgacaatt acgacggcct acactaggtt cgcgcctgcc
    24901 tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga ccgcctgaaa tatccgatga
    24961 aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg
    25021 acatcatcgc gaccaatatg cagcgcctga tcaaagagta cggcaacgag tctatctatc
    25081 tgaactatgg caccggtacg ctgggcggca ccatgacccg ctcctggccg ccgggaaata
    25141 ccctggtcgc gcgcctgatg aactgctgcg gcggttatct taaccattac ggtgactact
    25201 cttccgcgca aattgcggaa ggtttgaacc atacctacgg cggctgggca gatggtaaca
    25261 gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt tggtaataac ccaggcgaaa
    25321 cgcgaatgag tggcggtggg gtgacttact atcttgaaca ggcacgccag aaatctaatg
    25381 ctcgcatgat catcatcgat ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt
    25441 ggatccctat tcgtccagga acagatgccg cactggttaa cggtctggcg tacgtcatga
    25501 tcactaaaaa cctggtggat caggcattcc tcgataaata ttgcgttggc tatgacgaga
    25561 aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa agcttatatt ctgggtgaag
    25621 ggccagatgg cgtggctaaa acaccggaat gggcctcgca aatcaccggt gttccggcag
    25681 acaaaatcat caaactggct cgtgaaatcg gcagtaccaa accggcgttt atcagccagg
    25741 gatggggccc gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg
    25801 cgattctgac cggtaacgtt ggtattaatg gagggaacag cggcgcgcgt gaaggttcat
    25861 acagcttacc gtttgtccgt atgccgacct tggaaaaccc gatccagacc agcatttcga
    25921 tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacggcgctg cgtgatggtg
    25981 tgcgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat gccggtaact
    26041 gcctgattaa ccagcattct gaaatcaacc gtacccatga aatcctccag gatgataaga
    26101 agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa tatgctgaca
    26161 tcctgctgcc tgactgcacc gcttccgaac agatggactt tgcactggat gcatcctgcg
    26221 ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga
    26281 ccatctatga aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa cagtttactg
    26341 aaggccgtac ccaggaagag tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc
    26401 ctgaactgcc aacgtttgaa gagttccgca agcaggggat ctttaaaaag cgcgacccac
    26461 aagggcatca cgttgcttat aaagccttcc gtgaagatcc gcaggcaaac ccactgacta
    26521 cgccatcggg caaaattgag atttattcgc aggcgctggc tgacattgcc gctacctggg
    26581 aattgccaga aggcgatgtg atcgatccac tgccgatcta cacgccgggc tttgaaagtt
    26641 atcaggatcc gctgaacaaa cagtatccgc tgcagcttac aggtttccac tataaatctc
    26701 gcgttcactc aacttacggc aacgttgatg tgctgaaagc ggcttgccgt caggaaatgt
    26761 ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa cggcgataaa gtcaggatct
    26821 ttaacgatcg tggtgaggtt catattgagg cgaaagtgac gccacgaatg atgccgggcg
    26881 tggtcgcact gggtgaaggc gcctggtatg acccggatgc aaaacgtgtc gataagggcg
    26941 gttgtatcaa cgtactgacc actcaacgtc cgtctcctct cgctaagggg aatccgtcac
    27001 atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga
    27061 ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc ttgtaaagac
    27121 tacaaagatt tgacgccaga agtcagtttc cgccgcattt atgaatatgc tggcggcgac
    27181 tggcaggaag ataacggtgt ctggcaccag aacgtgtttg cctactatct gtcgatttcg
    27241 tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc cgagcggtgc gatgcataaa
    27301 cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca ttggctgccg ttactgtcat
    27361 atggcttgcc cgtatggcgc accgcaatat aacgaaacga aaggccatat gaccaaatgc
    27421 gatggttgtt atgaccgtgt tgcggagggt aaaaagccga tctgtgttga atcttgcccg
    27481 ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta aaaaacatgg cgatctggcg
    27541 gccgttgcgc cgttgccgcg agctcacttt accaaaccga atattgtgat caaacccaat
    27601 gccaatagcc gcccgaccgg ggataccact ggctatctgg caaacccgaa ggaggtgtga
    27661 gatgggaagt ggatggcatg aatggccgct gatgatcttc acggtcttcg ggcaatgtgt
    27721 ggcgggtggt tttatcgttc tggctttggc gctgctcaaa ggcgacctgc gagcagaagc
    27781 ccagcagcgt gttatcgcct gcatgtttgg cttatgggta ctgatgggca ttggctttat
    27841 cgcctctatg ctccatcttg gttcaccgat gcgcgccttt aactcgctca accgggtagg
    27901 ggcttcagca ctcagtaacg aaatcgccag cggttcgatc ttttttgctg taggcggcat
    27961 cggctggttg ctggcaatgc tgaaaaagct gtcaccggca ttgcgtacgc tatggctggt
    28021 ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg gtgcgtgtgt ataacagcat
    28081 tgataccgtt ccgacctggt acagcatctg gacgccgatg ggctgggcga tgcgtctgct
    28141 gccagccatt tctgtactgg cactggtggt gagtggcgtg atgtcagtga tgcagggcac
    28201 agagctggca actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg
    28261 tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc
    28321 acagctaaaa ggttatcagc ctgcggttcc gctactatcc gtctcattca ttctgcttct
    28381 ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc
    28441 cgtcgcaagc taatcagtaa caaccggggt ttcggccccg gttctctttt atttctgctt
    28501 cgttaacgtg tcataactgg tcatcaaatt acgataatca ggaatatggt tagagaacaa
    28561 cgtcgccagt ccttcaatat cattacgcca gtcacggtgc agctcgcaag ccacgccgaa
    28621 ccatgtcatc aattgcgcac cagcttgcga catgcgatcc cacgccgaat gccgggtaat
    28681 ttcattaaag gtccctgatg catcagtcac gacaaagaca tcaaaccctt cttcaatggc
    28741 tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt
    28801 tttacctgtc gctttgactg cttttacaaa atcttcgtta tcccaggcgt taatattgcc
    28861 cgggcgagca atgtaaggtg catcggggaa ttgtgctttc agttctagaa ctaatgggcc
    28921 gttaggaccg gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag
    28981 gtcacccagc gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga
    29041 aagtaaacca gcctggtgat caacaagcaa aacggcagca tcatttttat caagacgaac
    29101 atacggtttg gtcatcctct tctcctttcg agagtcagtg gtacgtctga aatcggtcag
    29161 acgcgatgca ttgctctgaa agcatagacg ggaaatatga gtttgctgtg accatgaaat
    29221 ttttcgactg aacgaagcgt atgaaatttg tgttagatca ataaaaacaa tcagatgaga
    29281 ttaatcatct cttcagcatt agcgttatga atgagtaata taaatcgata ataccgccgc
    29341 tgcgatatgc gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg
    29401 aaatttgaac ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt
    29461 tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc
    29521 tttggctcgc ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt
    29581 ttaccggcaa ccttgtcggt acattgctga cagggtatgt cattaaacgc attggcttta
    29641 accgcagcta ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc cttggcctga
    29701 tgattggttt ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga
    29761 tttgggtggt tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt
    29821 tgcttgctgc gtatatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca
    29881 gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt tacaggtttg acattggcag
    29941 ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac catgattcga
    30001 cgtcaattac ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta
    30061 tctcaggaat cgttctggga tctctatatg gcctgatgcc tctgtacctc aatcacaaag
    30121 gggtgagcaa tgccagtatt ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg
    30181 gacaatggcc gattggacgt ctggcggata agtttggtcg actgctggtt ttgcgtgttc
    30241 agg
//
LOCUS       DAJVPP010000045        30022 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVPP010000045 DAJVPP010000000
VERSION     DAJVPP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.045"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OQM34_001983"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OQM34_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1452442.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OQM34_001984"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OQM34_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1452443.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OQM34_001985"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OQM34_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1452444.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OQM34_001986"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OQM34_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1452445.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OQM34_001987"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OQM34_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1452446.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OQM34_001988"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OQM34_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1452447.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OQM34_001989"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OQM34_001989"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1452448.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OQM34_001990"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OQM34_001990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1452449.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADHI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OQM34_001991"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OQM34_001991"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1452450.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OQM34_001992"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OQM34_001992"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1452451.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OQM34_001993"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OQM34_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1452452.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OQM34_001994"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OQM34_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCS1452453.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OQM34_001995"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OQM34_001995"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1452454.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OQM34_001996"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OQM34_001996"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1452455.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OQM34_001997"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OQM34_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1452456.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OQM34_001998"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OQM34_001998"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1452457.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OQM34_001999"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OQM34_001999"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1452458.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OQM34_002000"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OQM34_002000"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1452459.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQMA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OQM34_002001"
     CDS             21622..22212
                     /locus_tag="OQM34_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1452460.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OQM34_002002"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OQM34_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1452461.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OQM34_002003"
     CDS             22684..23379
                     /locus_tag="OQM34_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1452462.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OQM34_002004"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OQM34_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1452463.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OQM34_002005"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OQM34_002005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1452464.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OQM34_002006"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OQM34_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCS1452465.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OQM34_002007"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OQM34_002007"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1452466.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OQM34_002008"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OQM34_002008"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1452467.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7036 a         7825 c         8254 g         6907 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcatacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcttggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ttggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccacat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatgtcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gatggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa gagaaaaatc ct
//
LOCUS       DAJVPP010000046        29937 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVPP010000046 DAJVPP010000000
VERSION     DAJVPP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.046"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..735)
                     /locus_tag="OQM34_002009"
                     /pseudo
     CDS             complement(1..735)
                     /locus_tag="OQM34_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(710..1510)
                     /gene="otsB"
                     /locus_tag="OQM34_002010"
     CDS             complement(710..1510)
                     /gene="otsB"
                     /locus_tag="OQM34_002010"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCS1452468.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1677..2663)
                     /gene="araH"
                     /locus_tag="OQM34_002011"
     CDS             complement(1677..2663)
                     /gene="araH"
                     /locus_tag="OQM34_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1452469.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2678..4192)
                     /gene="araG"
                     /locus_tag="OQM34_002012"
     CDS             complement(2678..4192)
                     /gene="araG"
                     /locus_tag="OQM34_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS1452470.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4262..5251)
                     /gene="araF"
                     /locus_tag="OQM34_002013"
                     /pseudo
     CDS             complement(4262..5251)
                     /gene="araF"
                     /locus_tag="OQM34_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            6048..6551
                     /locus_tag="OQM34_002014"
     CDS             6048..6551
                     /locus_tag="OQM34_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1452471.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6631..6882)
                     /locus_tag="OQM34_002015"
     CDS             complement(6631..6882)
                     /locus_tag="OQM34_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1452472.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6997..7083)
                     /gene="azuC"
                     /locus_tag="OQM34_002016"
     CDS             complement(6997..7083)
                     /gene="azuC"
                     /locus_tag="OQM34_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1452473.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7346..7669
                     /locus_tag="OQM34_002017"
     CDS             7346..7669
                     /locus_tag="OQM34_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1452474.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7840..8337
                     /gene="ftnA"
                     /locus_tag="OQM34_002018"
     CDS             7840..8337
                     /gene="ftnA"
                     /locus_tag="OQM34_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1452475.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8374..8613)
                     /locus_tag="OQM34_002019"
     CDS             complement(8374..8613)
                     /locus_tag="OQM34_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1452476.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8803..10014
                     /gene="tyrP"
                     /locus_tag="OQM34_002020"
     CDS             8803..10014
                     /gene="tyrP"
                     /locus_tag="OQM34_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1452477.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(10076..10741)
                     /gene="yecA"
                     /locus_tag="OQM34_002021"
     CDS             complement(10076..10741)
                     /gene="yecA"
                     /locus_tag="OQM34_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1452478.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10937..11023)
                     /locus_tag="OQM34_002022"
     tRNA            complement(10937..11023)
                     /locus_tag="OQM34_002022"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10987..10989),aa:Leu,seq:taa)
     gene            complement(11036..11109)
                     /locus_tag="OQM34_002023"
     tRNA            complement(11036..11109)
                     /locus_tag="OQM34_002023"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11075..11077),aa:Cys,seq:gca)
     gene            complement(11164..11239)
                     /locus_tag="OQM34_002024"
     tRNA            complement(11164..11239)
                     /locus_tag="OQM34_002024"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11204..11206),aa:Gly,seq:gcc)
     gene            complement(11391..11939)
                     /gene="pgsA"
                     /locus_tag="OQM34_002025"
     CDS             complement(11391..11939)
                     /gene="pgsA"
                     /locus_tag="OQM34_002025"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1452479.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11996..13828)
                     /gene="uvrC"
                     /locus_tag="OQM34_002026"
     CDS             complement(11996..13828)
                     /gene="uvrC"
                     /locus_tag="OQM34_002026"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1452480.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13825..14481)
                     /gene="uvrY"
                     /locus_tag="OQM34_002027"
     CDS             complement(13825..14481)
                     /gene="uvrY"
                     /locus_tag="OQM34_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1452481.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14777..14953
                     /locus_tag="OQM34_002028"
     CDS             14777..14953
                     /locus_tag="OQM34_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452482.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14940..15164
                     /locus_tag="OQM34_002029"
     CDS             14940..15164
                     /locus_tag="OQM34_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1452483.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(15197..15817)
                     /gene="sdiA"
                     /locus_tag="OQM34_002030"
                     /pseudo
     CDS             complement(15197..15817)
                     /gene="sdiA"
                     /locus_tag="OQM34_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(16047..16799)
                     /gene="yecC"
                     /locus_tag="OQM34_002031"
     CDS             complement(16047..16799)
                     /gene="yecC"
                     /locus_tag="OQM34_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1452484.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16796..17464)
                     /gene="tcyL"
                     /locus_tag="OQM34_002032"
     CDS             complement(16796..17464)
                     /gene="tcyL"
                     /locus_tag="OQM34_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1452485.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17479..18465)
                     /gene="dcyD"
                     /locus_tag="OQM34_002033"
     CDS             complement(17479..18465)
                     /gene="dcyD"
                     /locus_tag="OQM34_002033"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1452486.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18570..19370)
                     /gene="tcyJ"
                     /locus_tag="OQM34_002034"
     CDS             complement(18570..19370)
                     /gene="tcyJ"
                     /locus_tag="OQM34_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1452487.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19458..20009)
                     /gene="fliZ"
                     /locus_tag="OQM34_002035"
     CDS             complement(19458..20009)
                     /gene="fliZ"
                     /locus_tag="OQM34_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS1452488.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(20055..20774)
                     /gene="fliA"
                     /locus_tag="OQM34_002036"
     CDS             complement(20055..20774)
                     /gene="fliA"
                     /locus_tag="OQM34_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS1452489.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(20939..22591)
                     /gene="fliC"
                     /locus_tag="OQM34_002037"
     CDS             complement(20939..22591)
                     /gene="fliC"
                     /locus_tag="OQM34_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS1452490.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22857..24263
                     /gene="fliD"
                     /locus_tag="OQM34_002038"
     CDS             22857..24263
                     /gene="fliD"
                     /locus_tag="OQM34_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1452491.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            24288..24698
                     /gene="fliS"
                     /locus_tag="OQM34_002039"
     CDS             24288..24698
                     /gene="fliS"
                     /locus_tag="OQM34_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1452492.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24698..25063
                     /gene="fliT"
                     /locus_tag="OQM34_002040"
     CDS             24698..25063
                     /gene="fliT"
                     /locus_tag="OQM34_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCS1452493.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            25141..26628
                     /gene="amyA"
                     /locus_tag="OQM34_002041"
     CDS             25141..26628
                     /gene="amyA"
                     /locus_tag="OQM34_002041"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1452494.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26662..27075)
                     /locus_tag="OQM34_002042"
     CDS             complement(26662..27075)
                     /locus_tag="OQM34_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1452495.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            27262..28467
                     /gene="yedE"
                     /locus_tag="OQM34_002043"
     CDS             27262..28467
                     /gene="yedE"
                     /locus_tag="OQM34_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1452496.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28464..28697
                     /gene="yedF"
                     /locus_tag="OQM34_002044"
     CDS             28464..28697
                     /gene="yedF"
                     /locus_tag="OQM34_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1452497.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28804..29474
                     /locus_tag="OQM34_002045"
                     /pseudo
     CDS             28804..29474
                     /locus_tag="OQM34_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29514..>29937)
                     /locus_tag="OQM34_002046"
     CDS             complement(29514..>29937)
                     /locus_tag="OQM34_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1452498.1"
                     /translation="MIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYE
                     MRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADV
                     NGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7442 a         7734 c         7132 g         7629 t
ORIGIN      
        1 taagttgcgc cagttttggc ggcagtggcc cggcagcctg tttggctatt tctttcggtt
       61 caatgccgat cgggtagact tctgttcgaa atgcttttcc ccaggctgta tggctttttg
      121 cgctacgtgt cgtgacgcgg gtcaggttag aaaggcaatc caggaacgcc agacgatcgt
      181 tttctgtctg taaacccagc aaatcataat cacaaagctg ttcaagcaag gtgtcatatg
      241 tcggcagcgc gttgaaaatt tccggtgtcg ggaaaggaat atgcaagaaa aaaccaatgc
      301 gattattcac cccacgtttg cgtaattcat gcgcaaaggg caacaggtga taatcgtgga
      361 tccagataat gtcatcgtct tgcaacagcg gcagtaattt atctgccagc aacgcattta
      421 cgcgtagata gccgtcccag gcaggacgct gaaattgcac cagatcgagc cgataatgaa
      481 aagcgggcca gagaacggca ttggagaatt ggttgtagta ttcgtcaagg tcctgttcgc
      541 tgaggttaaa agaggcccac gtaatgttac cttttttagc ggctgatcct cattccctgt
      601 ttcaccactc cagccaaacc acagtccgcc tgcggctttc agtgccccca gtatgccaac
      661 ggcaaggcca ccggcactgg cggcgtgctc gtctggtggt gcaatccggt tagatactac
      721 gactaaacga ctcatagtca tcactcctgt tattttctct tttttgttgt aatgcggcgg
      781 ttatcatttc aagccagctc cagacatccg gcacacccgc cagtcgccat gatgcctgag
      841 ttgcacctgt gccaattttt actgacattc cgcccagtcg gttaacgact gcgaagccag
      901 attcatcggt taaatcatcg cccagaaata cgggcgttcg cccgataaaa ggagcttcct
      961 gcataaaggc tgcaatcgct tcacctttac tggtacctct cggtttgatc tcgacaacac
     1021 actttccctg ctgtaacgcc atttgtggcc agatctgagt aatacgttgc gctaatgtca
     1081 ttaatgcgtc ttcatgctgc ggagcctgac gataatgcag cgcaaaagcc atccctttcg
     1141 cctccagctc cgcgccggga tactgagcga tgaccgtatg cagttgcacg ctaatatcac
     1201 gcgcaatcgc atccggcaga tgaacgatat gtgttttacc attgatgtca cggcgctccg
     1261 ccccatgcac gcccgctaac gggaagcgat aaggttttgc cagtgcgtca agctccacca
     1321 ttgagcgccc tgatatcaat gccaatgcac catcacttgc ggttgccagt agctgtagtc
     1381 cttgcagaat attgtcaggc acgacgacct gatcgggatg cggtttgatt tccgccagcg
     1441 ttccatcaag atcaaaaaac caggcatatt tcgcggatag ttcaggggtt tcggttaacg
     1501 gttctgtcac ccggctctcc tccttctctt ttcttattgt gttgcgcaga ccgcgcttta
     1561 cagacatgta agtatagaca gtgtgacgga ggtcgccatt tgcaacgaag aacagccaac
     1621 cgttgtggcg gctggctgtg gtgggaaaaa acgttaaatt gttgtggaaa aaagcatcag
     1681 acagtgcgct tcgctttttg cttgtaacgg tcgaagatca ccgctgccag caggattaag
     1741 ccgcgaacca cgtactgcgc gaaaggagaa atattaagca ggttcatggc gttttccacg
     1801 gtgcctaaaa ttaagatacc cgccaccaca tatgagattt ttccgatgcc acctttcaga
     1861 gaaacgccac ctaaaacgca ggcggagata acaataagct cataaccaat cgacgtcatt
     1921 ggctgcccac tagtcatacg tgaagccaga ataattccgg ctatcgcaga taccaggcca
     1981 gagagaacaa agataataat tttggtgcga acaaccggta cacccgccag acgcgcagcc
     2041 tcttcgttcc cgccaatcgc cagggtatta cgaccaaagg tggttttatt cagcagcaaa
     2101 ccaaagataa tcagacacgc gacggtgagc cagattggcg caggcagacc gaaccagtta
     2161 gcgtaaccaa gggcaaagaa gctttcatct tcgataccga ccgctttacc gtctgaaatg
     2221 atgtacgcca gacctcgaac aatctgcatc gttgccagtg tcgtgatcag agcatttatt
     2281 ttcagtttgg cgataacaaa gccattgacc aggccacaga gaacgcccag caacaaaccc
     2341 gctgccacgc caatccacag gctttcagtc aggttgataa ccaccgccgt ggtgacaccc
     2401 gcacaggcaa ttacggaggc gacagaaagg tcaaagtcac cggaagcaag gcagaacaac
     2461 atgccacacg ccaccatccc cgacatggaa attgccaggc ccaacccttt catattaatg
     2521 aaggtggcaa aatttgggac aaaaatggca caggcgataa agagcaccgc aaacaccacc
     2581 agcatgccgt actgatccca gatacgcccg aagctgaatg acgacttagg tgcgccagac
     2641 cccgatgtag aaacagaaga catcatactc tccttactca ggcaactgcc tggctgactt
     2701 taggcatcgc aaggctcagt gcctgacgct catctgcctg ctcgtgtaac aattcaccgg
     2761 cgatttcgcc ttcccgcatc accacaatcc ggtcggcaac gccgaggact tcaggtaagt
     2821 cgctggaggc aaacagcacc gccacgccct gcgccgccag cgcataaatt acgttatata
     2881 tttcgtgctt agcgccaaca tcaatgccgc gcgtaggttc atccagcaaa atgaccttca
     2941 tctcttccga taaccagcgg cccagaatgg ctttttgctg atttccgcct gagagattca
     3001 tgatcagttg ctccgcgccc ggcgttttga tgttgagcga acgaatgtgc tgatcggcat
     3061 tgttttcttc ccaactgttg ttgattacac aaccgccgag cacatgtttg cgtctggcac
     3121 tgatgttgat attgtcgcga acggagtgca cgggaataat gccttccgct ttgcgagctt
     3181 ccgggcagag catcatgcct gcggcaatgg cgtggctcgg tttacgaata tcgatcggct
     3241 gttggtcgat ataaacctga ccggcggtga tttgcgtccc gccaaacaag cctttcatta
     3301 attcgctacg ccccgcccct accagaccaa acagcccaac aatttcacca ctgcgaaccg
     3361 ccagacttat tggcgtacgc acgcctagtg ctttcacagc atcaagacgc aggcgctcct
     3421 cgccataact acgaggttgc cagccgtaga tatcgccaat gtcgcgcccg accatcgcct
     3481 gcaccagcgc gtcgtggtca acctgctgca tatcggtaaa ggttttgaca taacgtccat
     3541 ctttaaagac agtgatggca tcgctgaggg caaatatttc ttccatacgg tgagaaacgt
     3601 ataagatcac ccgcccctct tttcgcagtt cacgaataac gcggaaaaga ttgccgattt
     3661 cgcgggcaga aagggagctg gttggctcat caaaggcgat aattttagcg ttacgcgcca
     3721 gcgctttggc gatttcaacc atctgccact gaccaatggc gagatatttc agcggcgtgt
     3781 ccgggtcaat atccatacca agatgtttaa gttgtaaacc cgcctcataa ttcagcaatg
     3841 agcgattcac aatgccgcct ttatgcggca gctggccgag atagatgttt tccgcgacgg
     3901 tcatttccgg cacgagatgc agttcctggt aaataatcgc cacacccgcg ttaagtgctg
     3961 cggtcgtgtc ggaaaaggac atttcctgcc cattaatcac tacagaaccc gtggttggcg
     4021 catagttgcc gctgaggatt tttaagagag ttgattttcc tgcgccattt tcacccatca
     4081 acgcatgaac ctgaccggca tagcagtcaa aactaatatc cgtcagcgcc ttaacgccgg
     4141 gaaacgtttt accgatgccg cgaaatgaga gatacggggt agactgttgc ataacgtctc
     4201 cgtgaatcac tggtcgttca caccccctct gcatcatgca gaggggaatt tttccggcaa
     4261 attacttacc gcctaaacct tttttctcca gttcttcttt aaagttgtca cgcgtgatca
     4321 gtaccacgtc tgtaacttcg gtaaattttg gcgtttcaac gtcttttgct acccagttgt
     4381 aaagcatttc gctggattta tagccatgta cgtccgggct tggcagcagg gaaccgtaga
     4441 agccggttgc ctgtgcttta gacagttcgc ttactgcatc cacaccgtta ataccaatac
     4501 cgatgatatc ggctgcttta aagccctgac cttcggttgc acgtacgccg ccaagtacgg
     4561 tgctgtcgtt cataccgacg atcagccaat gtttaacttc cggatgttga accagcattg
     4621 agttggcagc gtcaaatgcc cccgggatgt cgttagattt ggtaggtacc tgataaattt
     4681 gtttttccgg gaatcctgca gctttcagtg cgtccataga acccgttgta cggcggcggg
     4741 cggtatccag ttcgttggcg gtaatcgcca tcactgcgct ttctttgaca tcccagccac
     4801 gtttctgcat ctctttatac agttcctagc cctgacgttc gccaatttta gtcgccgcca
     4861 tcatcaccag aggaacggta tccattggct tacctttggc gttaacaaac tggtcatcca
     4921 ctgcaatgac tttcatatcg tagccacgcg ctttcgcgac gatggcagag ccgagtttcg
     4981 ggtccggagt acaaataacg aaaccttttg cgccactggc tgccaggctg tcgatcgcgt
     5041 tcaatgtttt ttcgccatcc ggcacggcaa tcttaataac ctcaaaccct aaatccttcc
     5101 cggctttatc ggcaaacttc cattcggtct ggaaccacgg ctcttccggt tgcttcacca
     5161 gaaaaccgag cttcaggttc tccgccatag cggattgtga cataacggct gccagaccaa
     5221 tggctgccag ggctttagta aatttgtgca tggttctctc cagctttagt gtcgttttgt
     5281 gtagtgcaaa aacgaatgac attcgttaaa ttaatcggaa aacaaagcat taccttttat
     5341 ctaaaagata agtgaccgtg ttgacatagt tttagcgaga aattaattct ctataggaga
     5401 gcaatatcac atcgcagaat tacagtgaga acgtgcataa atttagcggg aaaagacata
     5461 aggaaaagcc aatttgtcag acaaattgtt gaatgcacag cagattaatc cataagatta
     5521 gcctggaaat ccttgttgtc ttcggtaccc atgcgggatg tcttcttttt aaccagtcaa
     5581 taggccgcat tacctggcgt tgagtttttg aaatggtgta ataaccacaa ctcaaagatg
     5641 tggaaaatgc gcgtcattca tttcgccatt aattatcact gtgctcatta attaacagaa
     5701 cacgtataat gagagccatc tcgcaaaaat gaaaaaacgt tttataaaat catcacttca
     5761 tcatgaattc aaattcatcg attaatatca acaagataca aaaagcacta tcattaaaat
     5821 tcattgcatt tacattgatt tcaccaatga aatgtaaaaa tatataaact tgatgattta
     5881 agcattttct tataaccgtt cagacgttat tcttatttca gatcatcgtc agaattgact
     5941 ccacgatcac atttcggacc ggcagaaagg aattattctg caaacagtaa ttatggtgtt
     6001 ttgatttatc ttgcacctct ccacttctgg atataaggat attaggtatg gcaaccgctg
     6061 gaatgcttct caaactcaac tctcaaatga accgcgagtt ttacgcatcc aatctctacc
     6121 ttcacctgag taactggtgt tctgaacaga gtctgaacgg caccgccact ttccttcgcg
     6181 cccaggcaca gagtaatgtg acccaaatga tgcgcatgtt taactttatg aagagtgtcg
     6241 gcgctacccc catcgttaaa gccattgatg ttccaggtga aaaactgaac tctctggaag
     6301 aactgttcca aaaaacgatg gaagaatacg agcaacgttc aagcactctg gcgcagttag
     6361 ccgatgaagc gaaagaactg aatgatgatt caaccgtcaa tttcctgcgc gatctggaaa
     6421 aagaacagca gcatgatggc ctgttgctgc aaaccattct tgatgaagtg cgcagtgcga
     6481 aacttgcggg tatgtgccct gtgcagaccg accaacatgt tctgaatgtc gtgtcacacc
     6541 agctgcattg atcatacatc ggcgctaata cattgcgccg atgaagtttt tgagaaaccg
     6601 ctgcctcatc tgtttgaagc agcggttttt ttaatgggat tcaccctgtg gggtaaactt
     6661 gagttcaatc agcgcgatgg ctttttggat tgcccgcatg gtgaccgggt ctgcggcagc
     6721 gggatgatga gtaaagtcga tattcttcag ctgactggac attttttcac gaacttcaac
     6781 gggcgcgatt acatcgagaa catccagaat ttgtttgata accaactggc aagctaccac
     6841 atcagaaacc agttcctgat cggcattcag cggctgggac atcgtaaact cctgatagca
     6901 ttttgaaagc cgttatagta gcggcttcac atcttcagcg atagtcacat ccaccgtcat
     6961 caggacacaa aaaaacctgc cggagcaggt tttttgttat cggaacatat tgcctggcgg
     7021 tacgtctttg aacgtcttgc aatagttatt gaacatactt ttcaggattt tgcgcagttt
     7081 catcgcggca ctccgaccat ttgttataca ggtgttattg tctttgcgct tataatatga
     7141 caaccatcac aaaaatcaat ctttatgtga tacaaatcac ataaataccc ctttaatgtt
     7201 ataaaaatga taatcaaaaa acagcccccc tatttctgac acctacagat ggcaagaaat
     7261 agcgcctgcc aggcgtcttt tccggccatt gtcgcagcgc tgtgacgcgt aaaatagtgc
     7321 tttctcttac tcttctggct ggaccatgag acttctgatt ctgactcttt cattaataac
     7381 gctggcgggg tgtacggtta ctcgccaggc ccatgtgagc gaagttgacg cggcaacagg
     7441 tattgtacgg ttggtttatg accaggcttt tttgcagcat gcccataccg atcgttatgt
     7501 cagtcgcggg atcgccgatc gcgcatgcca gcaggaaggc tatacccacg cgatcccctt
     7561 tggtcagcca gtaggcaact gtagcctttt tgcgggttcc ttatgtttga atacggaatt
     7621 cactttgtca tatcagtgcc atcattccgc cttccctgtc tttctctaaa tattttaatc
     7681 agcgagggga tcttcgctga ttaaagaaat agcggtaatg cgttttattc ccattcgtat
     7741 ttttaataat tgaagtttat attttacctt ttgcaaataa taaaataaca aattatagtg
     7801 acgccacagc aacaaatata acctttgtgg agcactatca tgctgaaacc agaaatgatt
     7861 gaaaaactta atgagcagat gaacctggaa ctgtactctt cactgcttta tcagcaaatg
     7921 agcgcctggt gcagctatca taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc
     7981 caggaagaga tgacgcatat gcagcgtctg tttgattacc tgactgatac cggcaattta
     8041 ccgcgtatta ataccgttga atctccgttt gctgaatatt cctcacttga tgaattattc
     8101 caggaaacct ataaacacga acaattaatc acccagaaaa ttaacgaact ggctcatgct
     8161 gcaatgacca atcaggacta cccaacattt aatttcctgc agtggtatgt ttctgagcag
     8221 catgaagaag agaaactgtt caaatcgatt attgataaat taagcctggc aggcaaaagc
     8281 ggcgaaggtc tgtattttat cgacaaagaa ctctctaccc tcgacacaca aaactaatgc
     8341 tctccggcgg caggtttctg ccgccgtttc aaatcagtga cggcaaatct tactctgatc
     8401 ggtcgaaaaa ccttcttctg aaggtataaa tttgcctttt gctgccagaa acgctaccag
     8461 ctctcccgct gtcacccctt ctgccgagca cgtgtgaaaa cgtgcctgtt caccaaaacg
     8521 cgctttaatc gcagcttcca gactggcatg cgtatattgc tcgcctgatt caatcatcat
     8581 atttaacact tcatgaccgt gaatagagtc catcgttcct cctcaaaaaa agcctagcgt
     8641 agcgattacc gcttatgaag actttgcgcc agcgcaggac tgaatgcttt tattgtacat
     8701 ttatatttac acaatatgta acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg
     8761 ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa cagaaccctg
     8821 ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc aatgccgctg
     8881 gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct ttgggcgttg
     8941 atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc agataccggt
     9001 ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac gggcttcagt
     9061 atgatgttct taatgtatgc tctgactgca gcatacatca gcggtgccgg tgaattgttg
     9121 gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg cgtgctgttg
     9181 ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga tttatttaac
     9241 cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt gctgctaccg
     9301 catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc actgtctgca
     9361 atcccggtga tttttacgtc gtttggtttt cacggtagcg tgccgagtat tgtcagctat
     9421 atggatggca acattcgtaa gctacgctgg gtgtttataa tcggtagtgc catccccctg
     9481 gtggcatata ttttctggca ggtggcgacg cttggcagca ttgattcaac aacctttatg
     9541 ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg cgaaatggtg
     9601 gcctctccgc atgttgagct ggcggtgcat ttatttgctg atttagccct cgccacgtca
     9661 tttctcggcg ttgcgttagg cttatttgat tatctggctg atttgtttca gcgttcaaat
     9721 accgttggtg gacgattgca aactggtgca attacctttc tgccgccgtt ggcgtttgca
     9781 ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc gctggcggta
     9841 ctggcattga ttatcccttc actattgacc tggcaaagca gaaagcacaa tcctcaggcg
     9901 ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg tggtattgct
     9961 gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg gtgatcagat
    10021 agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatgtg acctcttaat
    10081 gcaggcagca ctgcttaaat ttcttaccac tgccgcacgg acaaggatcg ttacgccccg
    10141 gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc gccatccagt
    10201 atgcatgtaa atcaagcgcc gccagtcgaa tggcatctac gctctcttca aacgcttctg
    10261 gcgacatttt ctctacccgc tcgaagtttt cctcagtacc gtgcagcgca atcgcctcca
    10321 gcgctggttt taaagaatcg ggcaacgttg accagtcaga aagtgccacg ccccgcatat
    10381 agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct cgcaagccga
    10441 ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg gccatatgtt
    10501 gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccaacgc ggcacatagt
    10561 cagccccacc ccacacggca accagccact gttctggttc aatctcttgc ggagaactca
    10621 acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg tcagtgttgt
    10681 atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt cccgttttca
    10741 tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg gtccgtatca
    10801 gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt aacgacaagt
    10861 tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttatta ctttgatttt
    10921 attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg cgaacgccga
    10981 gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa gaaagtggag
    11041 gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata accgctttgt
    11101 taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg atgtctttta
    11161 aactggagcg ggaaacgaga ctcgaactcg agaccccgac cttggcaagg tcgtgctcta
    11221 ccaactgagc tatccccgca ttcatcaagc aatcagttaa tcacttgatt ttattatcgt
    11281 ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc gatgagtcaa
    11341 cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga tcactgatca
    11401 agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt cagtaccgca
    11461 gccacaaaga aaagtgcaat accgacgtac tcaacccaaa tgttcggacg ccacagcagc
    11521 caggccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga gacggccacg
    11581 ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat aatttcacgg
    11641 gcgatcatcg ttgccgccgg taatgtcacc caccagctgt gataatgctc ggttaccagc
    11701 accatggcga tagccacgag aactttatct gccacagggt caaggaaagc accaaaccgg
    11761 gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg
    11821 cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa gaccaataca
    11881 aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt aaattgcata
    11941 atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag agacatcaat
    12001 gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc acttttgcaa
    12061 tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc aacaacattt
    12121 gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt ttgaccttcg
    12181 cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga atatgctgga
    12241 taacatgcag cgcgggtgaa tctggcggca aactaaatcc ctcaccttcc ggctcaaaga
    12301 acagcgtttc cagtccagcc ttacgatctg ctcctttggc aacgccaagt agcagcggat
    12361 gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc gcaagctggc
    12421 ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg gctttaccat
    12481 aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg atgcctgtaa
    12541 tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc acacaggaag
    12601 cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc ttcacttccg
    12661 gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat tgctgcgaaa
    12721 gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa cgcgccctat
    12781 cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa agggaatcgg
    12841 cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt aaggtgcgca
    12901 tctggctgcc ttgtaaatag aactgaccta cgaaggtttc taccacctcg ctcagttccg
    12961 taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc tgacgaatga
    13021 acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg tcaaggtcgt
    13081 cgccggtatt ggaaacaaac tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc
    13141 gaatacatgc cgcttcttca aactccagat tctggctggc agtttccata cggctaatga
    13201 gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac tcgacctgct
    13261 gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag cgccctatct
    13321 ggtattgcag acacggacgc gagcgattgc gataaacact attttcgcac tggcgaatgg
    13381 ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc gggaacgggc
    13441 cgaaatattc acctttggca tgctttgcac cacgatgcat cgccagacgc gggtgggtat
    13501 cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg ttgtaacgcg
    13561 gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct gtatgcgtaa
    13621 ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt
    13681 tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg acatagataa
    13741 ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg gtttttaaaa
    13801 acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat tacacagacc
    13861 atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt tactgaacat
    13921 acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg agatctcatt
    13981 gaccttctgg cccttagtga tcatcagcat aatctgcaat tcacgttcag acaaactggc
    14041 aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt gagcgatatc
    14101 agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga cttcctgcgg
    14161 agccgcgcct ttgctgaggt agccagcagc acctgcctgc atgactttcg ctggtaaagg
    14221 gttttctgta tggaccgtaa gcatgatgat tttaacatca gctgtggaac gcgcgatttt
    14281 acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca ccacgtcaac
    14341 ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctctc cgacgacttt
    14401 tataccctta atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc
    14461 aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc tactgatagt
    14521 tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa tggtgggtat
    14581 tcgatttttc tctcgttttt gtcaattcga ttgtccacag ttatggtcac gcccgtcacc
    14641 aacggcttac cggatcgttt cacagtaagc cctacgatga aatgtgacaa aaattgactt
    14701 tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac ccgaacaata
    14761 cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg tattttatag
    14821 cactgccgat atcacgctca aaaaacaacc actgctattt ttagcaaagc ttatggtata
    14881 ctccgccgcc ttaacatttt tcaccacaaa ttttcattgc aacatgacga ggaaaataaa
    14941 tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat gaagtctcct
    15001 gcctgaaagc gatgctgacg ctgatgctgc aggcgatggg acaagctgac gcgggccgcg
    15061 tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat
    15121 tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa accggctgag
    15181 agcgtgcctt tcagccggtt attgacaaaa tcatcgctat ctctgctgat gtcttccctt
    15241 ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa attcatctct ggcgtcatca
    15301 ctatttcatc atttaaacgc atcagagcca tcagactttc gcgcaccagt aactgcattt
    15361 ttaattgcag ttcatcacta agaatgggta tttcgcgcgt gctgcaacgg gaaaaggaca
    15421 aaaagcccag cgcccgattt ggcagcatta aatactgagt gacaccgcgg cgtaaaccat
    15481 gtgcgtgcgc ggcttcccat aatggctgtg cttcgctgaa taagtcatca ttccacatta
    15541 aatggccctg actaaagttt tcagggttca gcacaggatc aattgcgaga aaattttttg
    15601 cctgataata actaacccac gactcagggt aattggtgta gaaagccact ttaggtcgcg
    15661 tgaatggtac cgggtggcga acacataacg aatagtaatc gtactccagc tgctgagcct
    15721 gaagctctat ttcatggtag gcctcttctg cggcctccat cctctgaaaa cgcaacagca
    15781 tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt aaaccgcaac gcccctgaga
    15841 gtgagtatca tttataatga taattcaaac tatcacataa ataagattta tatataattt
    15901 atattattca ggcaatgaat tacttttgca agccatcgca ttctcttatg ttattaatga
    15961 gttatgctga tttgttaagc agttttatca cgcttgaaat ggcgtcccgc cccgacaggt
    16021 gaatcgtcgg ggctgatttt ttcttattat tgcagcagaa gcttctcgag gaactggcgg
    16081 gtgcgaggct gctggggatc ggcaaataac gcttttgctg ccccctgctc gactatccgc
    16141 ccctggtcca taaagatcgc ccggtccgca acatcccggg caaagctcat ttcgtgtgtc
    16201 acaatcacca tcgtgcgctt ttcctgcgcc agctggcgga tggtgttcag gacttcgccc
    16261 accagctctg gatccagcgc cgacgttggc tcgtcaaaca aaatcacctc aggacgcatt
    16321 gccagtgcac gcgcaatcgc aacacgctgc tgttgaccgc cagacaaacg acgtggatag
    16381 ctggtttctt taccggccag cccaactttc gccagcagct cgcgagcgcg cgccgtggcc
    16441 tcttctttcg gttcgccttt gacgatcacc ggcccttcaa taatgttttc cagcaccgta
    16501 cgatgcggaa acaaattaaa gttctggaag acaaacccga cgtgctgacg caactggcga
    16561 atcagcgatt tttgctgact taatgaacgt gcagtatcaa tagtgatatc gccaacggtg
    16621 atcgttcccg cttccggttg ttccagcaga tttatgctac gtagcaatgt ggttttgccg
    16681 gaaccactcg gaccgataat tgccaccact tcgccaggct ttacctcgag gtcgataccg
    16741 tgcagcaccg tctgaccgtg gaattttttc accaggttct taacttcaat ggcactcatt
    16801 ttggttctct ctcctggcga ttaagttgat tctcaaaatg gttctgcaac gtcgataaca
    16861 cagtcgccat gatccagtag atgagcgaag ccgccaggta catggtgaac acctccagcg
    16921 tgcgcgaggt aatcaactgc gcctgacgga acagctccgg cacctggatt gtcgcggcca
    16981 gcgacgtatc ttttaccaga ctgataaagc tgttcgacag cggcggcaac gccacccgcg
    17041 ccgcctgcgg caaaatcgcg cgacgcatgg tctgccacgg cgtcatacca atactggcag
    17101 cggcttccca ctgaccttta tcaatagaag aaatggcggc acgcagcgtt tcggcggcat
    17161 aggcggcagt gttcagcgac agaccaatca ttgctgacgg aatgggatct aattcgatac
    17221 caaactgcgg caggccgtaa tagatcataa acagttgtgc gatgagcggc gtaccacgaa
    17281 aaatagagat ataaaaacgc gccagccagc gcacgggcca gattggcgac aggcgcatca
    17341 gcgcgagaat aaacccgagt agtaagccaa aaaacatgcc gccgatactg agttgcaggg
    17401 tatacccagc ccctttcaac aggaacggca aggaatcaat aaccagttgt atactttctt
    17461 gcatgagcgt tcccggagct aaacgtgggg atgataggcg aacagcgcag gcgcgccgcc
    17521 ggtatgaata aacagaatcg gcccttcatc tttgaagcgt ttctgactga taccgtcaat
    17581 cagccccgcc atcgcttttc cggtatacac aggatcaagc agaatgcctt ccagacgcgc
    17641 cagcaatttc actgcttcca tgccttcgtc gttcggcacg ccgtagccag gtgcaaaata
    17701 gtcatcccag agtaaaattt ccgctgatgc ggtcagctcc agttctttcg caatcgcctg
    17761 ttgtaggtta accactttcg gcaattgatc ggcaacggaa cgcgacacgg tcacgccaat
    17821 cagttcgctt tcaggcatca ggtgttccag cccaacagcc agtccggcgt gagttccggc
    17881 actgcccgat gcgactacca ccgacgaaat attaaccgcc ccttcacact gttgcgcgat
    17941 ttccagcgca ctctccacat aacctagcgc gccaagagca ttagaaccgc caaccggaat
    18001 gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc agttcttcca gttgggcatt
    18061 gggatcggtc agtgcgtcgc acatttcaat ctgggtattg aacagatcca gcaacaaacg
    18121 attgccgttg gttaaatagt tttctgcggt tgtgccaata ggattttcca gcagcgccac
    18181 gcagtgcaga ccgagtttcg ccgcgactgc ggcagtctgg cgcacatggt tagactggat
    18241 cgcgccggca gtaatcagcg tatcggcacc ttcgcgcagg gcatctgccg cgagaaattc
    18301 cagcttacgt aatttattgc cgcccattgc catgggggtg acctcatccc gtttgatgaa
    18361 aatttcccgt ccaagataat cagaaaagcg cggcagatat tcgagcggcg ttggcgcgcc
    18421 gataaactcc aaccgtggaa aacgggttaa attatgcagt ggcataacag cctccgatgt
    18481 gtgttgttgt gattttctta ttatgcacgc tgaaaacgcg taaataaaaa aggcgctagt
    18541 gaaagcgccc ttttttgtca ttatgctgat tatttggtca catcagcacc aaaccatttt
    18601 tcggaaagag cttgcagagt gccgtctttt tgcatttccg caattgcatc attcaccgct
    18661 ttcagcaggt cttcatttcc tttacgcagc gccacgccag actcctgacg ggagaatgct
    18721 tcaccggtta ctgccagcgt atcgttggtt ttcttcacca gatccagcgc cgccagacga
    18781 tcaacgagga tcgcatcgat acgccctacg cgcagatcct gatatttggt cgggtcatca
    18841 tcataggtac gcacatcgac accctgaaca ttctgccgca gccactcttc atagttggtg
    18901 cccagaccga cacccacttt tttgcctttc agatcagcag ccgttttaat ggtgccttcg
    18961 ttaccttttt tcaccagcgc ctgaataccg gaaatggtgt aaggggtaga gaaatcgtat
    19021 tttttcttgc gctcgtcaga aatggtgacc tgattaatca ccacatcaat gcgtttagag
    19081 tccagcgacg ccagcatacc gtcccatttg gtcggtttta gtgacgcctc aacgccaaga
    19141 tgttttgcca gctgttgggc aaattccact tcaaaaccgg ttaatttgcc gtcatccccc
    19201 tgaaaactga acggcggata agtgccttcc agccctacca gcagcgtgcc gctctctttg
    19261 actttattaa gcagaccttc atctgcaaaa cttttaacgc tcatgcccgc aaccagcgcc
    19321 acggccatca cacccatcaa tgcctgacgt cccagatgtg ctaatttcat attcaccccg
    19381 aatgttgtta tgtctgtttg cagtgtagag ccattgtttg taaacacaaa aacaactccg
    19441 ctacatctta ttcttattta atatatatca gaagcaggca ggctggagga tttctgcaca
    19501 agtccggtac aggtttggcg ctgatagtgt tgatacttcc gtaatgcgat gcggtaattg
    19561 ttggtgctgg tggtgggcag ccacggggcg aggatttcat caaggaaacc gtcctgcagc
    19621 tgatcgaggg aaatattttg ctcatgtaga tgattaccca aacggcgcag acgaacgaca
    19681 tattcacgca ccgtacctgg gctcatttca gtttgctcaa aaagaaattt cttaaagccg
    19741 ataatatcga aaaaatcact ctgcgttttg caatgtaaat caccgcaaaa tcggcacaat
    19801 gccgcccatg attgttgttc cgtttgccag cctttttcat caagcagcgt gtccaggcgg
    19861 gaaatctcga ttttattcac tattttgccg tcgcgaacta aggtaatgcg atcgagtaat
    19921 ttacggcaat gcgcgcaatg ggtctggctg tgtttaaagt ctttaagata gcggcttaat
    19981 ggccgtcttt tcaggtgctg caccatcatt aagaactcct ggtagtcaaa gttaaagtgc
    20041 ggcatttact gacgttataa cttacccagt ttagtgcgta accgtttaat ggcctggctg
    20101 tgtaactgac tgacccgcga ttccccgacc tccagcaccg cgccaatctc tttgagattc
    20161 agctcttcct ggtaatagag ggttaatacc agtttttcgc gctccggcaa cgtttcgatg
    20221 gcttccatca cccgctggcg cagattactg tccagcagtt gttgtagcgg gttttctcgc
    20281 tgatgatcat cagtaaccag ttcgatgcta tcgccgtgct cttcacgcca ctcatcgtag
    20341 gagaagagct ggctgttatt ggtgtcgagc agcatttgac gataatcggc aatatcgatc
    20401 cctaaacgtt ccgctacctc agtttccgtg gcgttgcggc caagttcctg ctccagttgc
    20461 cctattgcct gtgccacttc acgcgcgttg cgtcgcacgc tgcgcggcac ccagtcacgg
    20521 ctgcgaagtt catccagcat agcgccacgg atacgctgca ctgcgtaagt tgtaaatgcc
    20581 gttccttgta gggcgtcata gcgttcgaca gcattaagta acccaatgcc gcccgcctgt
    20641 agcagatcgt caagttccac gctcgcgggc agtcgaacct gcaggcgcaa tgcttcgtga
    20701 cgcaccagcg ggacataacg ctgccacagc gagtgtttat ccattacacc ttcagcggta
    20761 tagagtgaat tcacgataaa cagccctgcg ttatatgagt tatcggcatg attatccgtt
    20821 tctgcagggt ttttaatcgg acgattagtg ggtgaaatga ggggttattt gggggttacg
    20881 caaaaagccg aatgtaaaaa accccgccgg tggcggggtt tgagaggcat tcagacgatt
    20941 aaccctgcag cagagacaga acctgctgcg gtacctggtt ggcttttgcc agcacggagt
    21001 taccggcctg ctggataatc tgcgctttcg acatgttgga cacttcggtc gcatagtcgg
    21061 cgtcctgaat acgggactgc gcttcagaca ggttggtagt ggtgttgttc aggttggtga
    21121 ctgcggaatc caaacggttt tgcaccgcac ccagggagga gcggaatttg tcgatggagc
    21181 tgatggcttc atccagagct ttcaggggat cggctgtcgt agctgctttt gtttcagcat
    21241 ctgtcgtcaa tttaccatca gcatctttat aaactgcctt accgctgccg ttagtcactg
    21301 aaccatcatc ctgtaggtaa aaatctacag tatcagatgt ggtcaacgcc tcgttgttat
    21361 caacataaac cgaatcaaca ccttttgtta cagcgccagt tgtgccgttg acgctataac
    21421 ctgcagtagt gaaatcaaca ttgtttcccg aggttgatac agctttatcg aaagctgcct
    21481 gatcaacttt tgcacctgtt actgaataag taatagtgtc cggcgtagta ctattaccgg
    21541 taaaggagat agaggtaccg ccaatgtcaa tggttgtcgc tttggtatta ttggctgcaa
    21601 gtgtggacag actagcctca gtcaaagaag caccagagcc gtttttcgtt aagcgcccag
    21661 ttgtatcaat gtagagttta tctccattgc ttgccgtaat tttaccatca ctgctgatct
    21721 gaacatcctg tgcagaacca tcgatagtaa tagtgccctt agcggtatca ccaactcccg
    21781 gggtcaaata tttctgaaca tcggcagctg aagccgttgt ggtatcaaaa gagtatgact
    21841 tacttgcact gtcatacttg taattcgttg cactagccgc cgttccgaag ccgttattaa
    21901 tggttgcctg aacagtatca ccatcactaa ctccagccag caaatcagac gctttagcag
    21961 catcgtaacc cgcactcaca gtatatttgt tgccgaccac tgttgcatta gcagctacca
    22021 agtcagcttt agatgcagca gtattagcca cagccccgcc accattcaca ttaaacccat
    22081 tcagccccag cgtatcagag tcaattttct tcagatcaat agtgatagtc tggccgtcat
    22141 tcgcaccaac ctgaattttc atcgaaccgt cttttgccag tacgttcacg ccgttgaact
    22201 gggtctgacc ggatacgcgg tcaatttcgt cgagacggga tttgatttcg tcctgaatgg
    22261 agtccagatc cgaatcagag ttagtcccgg tagaagcctg aaccgtcagc tcacggatac
    22321 gctgtaagtt gttgttgatt tcggacagcg cgccttcagt ggtctgtgca acagaaatac
    22381 cgtcgttggc gttacgtgca gcctgagtca ggcctttaat gttagaagta aaacggttag
    22441 caatcgcctg acccgcggcg tcatccttcg cgctgttaat acgcaagcca gaagacagac
    22501 gctcgataga actcgacagc gcagactggt tcttgttgat attattttga gtgatcagcg
    22561 agaggctgtt ggtattaatg acttgtgcca tgattcgtta tcctatattg caagtcgttg
    22621 attacgtttt gggtttccac ccgtcggctc aatcgccgtc aaccctgtta tcgtctgtcg
    22681 taaaacaacc tttagaattt ttttcacaaa cagccatttt ttgttagtcg acgaaatact
    22741 cttttctctg ccccttattc ccgctattaa aaaaaacaat taaacgtaaa ctttgcgcaa
    22801 ttcaggccga taaccccggt attcgtttta cgtgtcgaaa gataaaagga aatcgcatgg
    22861 caagtatttc atcgctggga gtcgggtcag gtctggattt aagttcaatc cttgatagcc
    22921 tcaccgccgc gcaaaaagcg acgctaaccc ctatttcaaa tcagcaatcg tcgtttaccg
    22981 ctaaacttag cgcctacggt acgctgaaaa gcgcgctgac gactttccag accgccaata
    23041 ctgcattgtc taaagccgat cttttttccg ccaccagcac caccagcagc accaccgcgt
    23101 tcagtgccac caccgcgggt aacgccatcg ccgggaaata caccatcagc gtcacccatc
    23161 tggcgcaggc gcaaaccctg accacgcgca ccaccagaga tgatacgaaa acggcgatcg
    23221 ccaccagcga cagcaaactc accattcaac aaggcgacga caaagatccg attaccattg
    23281 atatcagcgc ggctaactca tcgttaagcg ggatccgtga tgccatcaac aacgcaaaag
    23341 cgggcgtaag tgcgagcatc attaacgtgg gtaacggtga atatcgtctg tcagtcacat
    23401 caaatgacac cggccttgat aatgcgatga cactctcggt cagcggtgat gatgcgctac
    23461 aaagttttat gggctatgac gccagtgcca gcagcaacgg tatggaggtc tcggtcgccg
    23521 cccagaatgc gcagctgacg gtcaacaacg tcgccatcga gaacagcagc aacactatca
    23581 gcgacgcgct ggaaaacatc accctgaacc tgaacgatgt caccacgggc aaccagacgc
    23641 taaccatcac tcaggacacc tccaaagcgc aaacggcgat taaagactgg gtgaatgcct
    23701 ataactcgct aatagatacc ttcagcagcc tgaccaaata caccgccgta gatgcgggag
    23761 ctgatagcca gagttctagc aatggcgcac tgctcggcga ctccacgctg cggacgattc
    23821 agacgcagtt gaagtcgatg ctgagtaata ccgtcagttc ttccagctat aaaacgctgg
    23881 cgcagattgg tatcacgacc gatcccagcg atggcaaact ggaactggat gccgacaaac
    23941 tcaccgctgc actgaaaaaa gatgccagcg gcgtaggtgc attgattgtt ggcgatggta
    24001 aaaaaaccgg catcacaacc accatcggca gcaacctgac cagttggctt tcgacaacgg
    24061 gcattattaa agccgctacc gatggcgtta gtaaaaccct gaataagtta actaaagact
    24121 acaacgccgc cagcgatcgc attgatgcgc aggtcgcgcg ctacaaagaa caatttaccc
    24181 aactggacgt tttaatgacc tcgttaaaca gcaccagcag ctacttaacg cagcagttcg
    24241 aaaacaacag taattccaag taatcattat tcatcgggag acaggtcatg tacgcggcaa
    24301 aaggcaccca ggcctatgca caaattggcg tcgaaagcac cgtaatgagc gccagccaac
    24361 agcagttggt caccatgcta tttgatggag tgctgagcgc actggttaga gcgcgactgt
    24421 ttatgcagga caacaatcag caaggcaaag gcgtctcttt gtcaaaagcg atcaacatca
    24481 ttgagaacgg actgcgggtg agtcttgatg aagagagcaa agacgaatta acccaaaact
    24541 taattgctct ttatagctat atggtcaggc gtttgctgca agccaattta cgcaacgatg
    24601 tctccgcagt cgaagaggtg gaagcattaa tgcgtaatat tgccgatgcc tggaaagagt
    24661 ctttactatc cccttcttcg attcaggacc cagtctgatg aacaatgcac cgcatttgta
    24721 tttcgcctgg caacaactcg tcgaaaaaag ccagctcatg ttacgcctgg caacggaaga
    24781 acaatgggac gaactcatcg ctagcgaaat ggcgtatgtg aatgcggtgc aggagatcgc
    24841 acacctgact gaagaggttg ccccgtccac tacgatgcag gagcagctcc gcccgatgct
    24901 gcacctgatt ctcgacaacg aaagcaaggt aaagcagtta ttacagattc ggatggatga
    24961 actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg gtgttaagtg cctatggcga
    25021 tcagggcggc tttgtgctgg ctccgcagga taacctcttt tgaatcttaa tgagccgatg
    25081 gctcgcgaat aatccgatta cggctacgct tctaatattc cccttgaatg gagtcgaaga
    25141 atgcgtaatc ccacgctgtt acaatgtttt cactggtatt acccggaagg cggtaagctc
    25201 tggcctgaac tggccgagcg cgcagacggt tttaatgata ttggtatcaa tatggtctgg
    25261 ttgccgcccg cctataaagg cgcatcgggc ggctattcgg tcggctacga ctcctatgat
    25321 ttatttgatc taggtgagtt tgatcagaaa ggcagcatcc ctactaaata tggtgataaa
    25381 gcacaactgc tggccgccat tgacgccctg aaacgtaatg acattgcggt gctgctggac
    25441 gtggtagtca accacaaaat gggcgcggat gaaaaagaag ctattcgcgt gcagcgtgta
    25501 aatgctgatg accgtacgca aattgacgaa gaaatcattg agtgtgaagg ctggacgcgt
    25561 tacaccttcc ccgctcgtgc cgggcaatac tcgcagttta tctgggattt caaatgtttt
    25621 agcggtatcg accatatcga acaccctgat gaagatggca tttttaaaat tgttaacgac
    25681 tataccggag aaggctggaa cgatcaggtt gatgatgaac tcggtaattt cgattatctg
    25741 atgggtgaga atatcgattt tcgcaatcat gccgtgacgg aagagattaa atactgggcg
    25801 cgctgggtga tggaacaaac gcaatgcgac ggctttcgtc ttgatgcggt caaacatatt
    25861 ccggcatggt tttataaaga gtggatcgaa cacgtacagg aagttgcgcc aaagccgctg
    25921 tttattgtgg ctgaatactg gtcgcatgaa gtcgataaac tgcaaacgta tattgatcag
    25981 gtggaaggca aaaccatgct gtttgatgcg ccgctgcaga tgaaattcca tgaagcatcg
    26041 cgcatgggtc gcgactacga catgacgcag atcttcactg ggacgctggt ggaagccgat
    26101 ccttttcacg ccgtaaccct cgttgccaac cacgacaccc aaccgttgca agccctcgaa
    26161 gcaccggtcg aaccgtggtt taaaccgctg gcgtatgcct taattttgtt gcgggaaaat
    26221 ggcgttcctt cggtgttcta tccggacctc tacggtgcac attacgaaga tgtcggtggt
    26281 gacgggcaaa cctatccgat agatatgcca ataatcgaac agcttgatga gttaattctc
    26341 gcccgtcagc gtttcgccca cggtgtacag acattatttt tcgaccatcc gaactgcatt
    26401 gcctttagcc gcagtggcac cgacgaatat cccggctgcg tggtggtcat gtcgaacgga
    26461 gatgatggcg aaaaaaccat tcatctggga gagaattacg gcaataaaac ctggcgtgat
    26521 tttctgggga accggcaaga gagcgtagtg acagacgaaa acggcgaagc gaccttcttt
    26581 tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg tgatttaaat ccatccccgg
    26641 cggcaagccg gggagatttc attacggcag tggcgtcggt aacggcgctt tatccagcgc
    26701 ggcggcacac tctgccgtcg ggcgatccac acgctccatc gtcataccat cgtactcaat
    26761 ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt acggtaacgt tagtgagatc
    26821 atcagacatc aatgtcaact tacccggcac ggcaatcccc cgctgccact gacggcaatc
    26881 caaggtatcg ccctctttag tgacaatcag gctgcctatc gcttccggac tgaccagcgc
    26941 acgttgcggc cctttggtct gccagtagcc cgccagccag tccggcgcgg gggttttcac
    27001 gacattgtta tagttttcga cttcggcaca gcccgccagc aacatgagcg cgcctgcaat
    27061 tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag aggattgtgg cattaaagct
    27121 ctgatgtcgc cagcctttcc aggttcagag agaaatttga tctaactatt atttttgtga
    27181 attattaccc cgaaaataaa tttgcatggt gtatgattcg cggctcttgt ttcaaacctt
    27241 ctgagttcag aggctacatt catgtcatgg cagcaattca aacacgcctg gttgattaaa
    27301 ttctgggcac ccatccctgc ggtcatcgcg gcgggtattc tctccactta ctattttggc
    27361 attactggca ccttttgggc tgtcacgggt gaatttaccc gttggggcgg tcagctcctg
    27421 cagctgtttg gcgtccatgc tgaagagtgg ggttatttta aaattatcca tctggaagga
    27481 tcgccattaa cccgcatcga cggcatgatg atcctcggta tgtttggcgg ctgctttgct
    27541 gctgcgctgt gggccaacaa tgtcaaacta cgcatgccac gcagccgtat ccgcattatg
    27601 caggccatca ttggcggcat tatcgccggt tttggcgcgc gtctggcaat gggctgtaac
    27661 ctggcggcgt tctttaccgg cattcctcag ttctcgctgc atgcctggtt ctttgccatc
    27721 gccactgcca ttggttcatg gtttggcgcg cgctttaccc tgctgcccat cttccgtatt
    27781 cccgtgaaaa tgcagaaagt ttctgccgcc tcaccgctga cgcaaaaacc ggatcaggcg
    27841 cggcgtcgtt ttcgtctcgg gatgctggtc tttttcggca tgctgggctg ggcgctgctc
    27901 acggcgatga accaaccaaa actggggctg gcaatgctgt ttggcgtcgg ctttggttta
    27961 ctgattgaac gtgcgcaaat ctgctttact tcggcgttcc gcgatatgtg gatcaccgga
    28021 cgtacccata tggcgaaagc aatcattatc ggtatggcgg tgagtgccat cgggatcttc
    28081 agttacgtac agttaggcgt tgaacctaaa atcatgtggg cgggaccaaa tgcggtaatt
    28141 ggtggtttac tttttggttt tggcatcgtg ctggcaggcg gctgcgaaac cggctggatg
    28201 taccgcgcgg tagaaggcca ggtgcactac tggtgggtcg gtctgggcaa cgtgatcggc
    28261 tcaacgattc tggcgtatta ctgggatgat ttcgctccgg cgctggccac cgactgggac
    28321 aaaatcaacc tgctgaaaac ttttggtccg atgggaggcc tgctggtgac atatttgctg
    28381 ctgtttgctg cgctgatgtt gattatcggc tgggaaaaac gcttcttccg ccgtgaggca
    28441 ccgcagactg ctaaggagat cgcatgaaaa atatcgttcc tgattaccgc cttgatatgg
    28501 tgggtgaacc ctgcccttat ccggcagtcg caacccttga ggcgatgccg caattaaaaa
    28561 aaggtgagat cctggaagtg gtgagcgact gtccgcagtc gatcaataat attccactgg
    28621 atgcgcgtaa tcacggctac acggtgctgg atattcagca agacgggccg accattcgtt
    28681 atttaattca gaagtaatcc ctcattcccg ctgtggccca cagcgggaac atcgttacct
    28741 ctgacacata gattttaccc gccccctctc ctattctttt cagtatgatc tcaggaggtt
    28801 gctatgtgtg gacgctttgc ccaatcccaa acgcgtgagg attaccttgc gcttctcgcg
    28861 gaagatattg aacgcgatat tccctacgat cccgaaccca ttggcagata caatgtcgcg
    28921 ccgggaacca aagttcttct gctcagtgaa cgtgatgaac accttcatct ggatccggtt
    28981 ttctggggat atgctcccgg atggtgggat aaaccgccgc tgattaacgc ccgcgtagaa
    29041 actgcctcaa ccagtcgcat gtttaaaccg ctctggcaac atggtcgggc aatctgtttt
    29101 gccgatggct ggtttgagtg gaaaaaagaa ggcgacaaaa aaagccttat tttatctatc
    29161 gcgctgacgg acaacctgtt tttatggccg cgataggtag cataccgttt gagcgtggtg
    29221 acgaagccga aggatttttg atcgtcactg cggcggcaga tcaaggtctg gtagatattc
    29281 atgaccgccg cccgctggtt ctgtcgccag aagctgcacg ggaatggatg cggcaagaca
    29341 ttggtggtaa agaagcctca gaaatcgcta ccagaagctg tgtgccagca aaccagttca
    29401 cctggcaccc tgtgtcgcgc acggtgggta atgtcaaaaa ccagggcgcg gagttaattc
    29461 aacctgtttg ttgacatcct ccacgccctg aatgacgagg acccctgcta cgttcaggct
    29521 gtcgcctgaa tcatttcggt gggttcctgc ttcaacgagc ggcctgactg caccatctct
    29581 ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac gagctccatt tacatcggcg
    29641 ttcgctgtat atccacatac ctggcatctg aatttacttt gtgacaggcg attttctttc
    29701 gctgtatgac cacagcacgc gcaacgctgg cttgtgtacg ctggcggaac agcaagcacc
    29761 tgaccgccac gccagagctg cttgtactca agctggcggc gcatttcata ccagccctga
    29821 tccagtatcg aacggtttaa acctgatttt gcccggacat tgcgccccgg ctggctgacc
    29881 gtacccgctg ctgactttga catgttgctg accttcaaat cctcaatgac tatcatt
//
LOCUS       DAJVPP010000047        28961 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVPP010000047 DAJVPP010000000
VERSION     DAJVPP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.047"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..899
                     /locus_tag="OQM34_002047"
                     /pseudo
     CDS             1..899
                     /locus_tag="OQM34_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1292..1633)
                     /locus_tag="OQM34_002048"
     CDS             complement(1292..1633)
                     /locus_tag="OQM34_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1452499.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1646..2518)
                     /gene="copD"
                     /locus_tag="OQM34_002049"
                     /pseudo
     CDS             complement(1646..2518)
                     /gene="copD"
                     /locus_tag="OQM34_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(2522..2896)
                     /gene="yobA"
                     /locus_tag="OQM34_002050"
     CDS             complement(2522..2896)
                     /gene="yobA"
                     /locus_tag="OQM34_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1452500.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3035..3265
                     /gene="holE"
                     /locus_tag="OQM34_002051"
     CDS             3035..3265
                     /gene="holE"
                     /locus_tag="OQM34_002051"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1452501.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3367..4023
                     /locus_tag="OQM34_002052"
                     /pseudo
     CDS             3367..4023
                     /locus_tag="OQM34_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4047..4709
                     /gene="exoX"
                     /locus_tag="OQM34_002053"
     CDS             4047..4709
                     /gene="exoX"
                     /locus_tag="OQM34_002053"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1452502.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4706..6766)
                     /gene="ptrB"
                     /locus_tag="OQM34_002054"
     CDS             complement(4706..6766)
                     /gene="ptrB"
                     /locus_tag="OQM34_002054"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS1452503.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(6976..7635)
                     /locus_tag="OQM34_002055"
     CDS             complement(6976..7635)
                     /locus_tag="OQM34_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1452504.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(7962..8318)
                     /locus_tag="OQM34_002056"
     CDS             complement(7962..8318)
                     /locus_tag="OQM34_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1452505.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8385..8675)
                     /gene="yebG"
                     /locus_tag="OQM34_002057"
                     /pseudo
     CDS             complement(8385..8675)
                     /gene="yebG"
                     /locus_tag="OQM34_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            8809..9987
                     /gene="purT"
                     /locus_tag="OQM34_002058"
     CDS             8809..9987
                     /gene="purT"
                     /locus_tag="OQM34_002058"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1452506.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10043..10684)
                     /gene="kdgA"
                     /locus_tag="OQM34_002059"
                     /gene_synonym="eda"
     CDS             complement(10043..10684)
                     /gene="kdgA"
                     /locus_tag="OQM34_002059"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1452507.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10721..12532)
                     /gene="edd"
                     /locus_tag="OQM34_002060"
     CDS             complement(10721..12532)
                     /gene="edd"
                     /locus_tag="OQM34_002060"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1452508.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALAFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12767..14242)
                     /gene="zwf"
                     /locus_tag="OQM34_002061"
     CDS             complement(12767..14242)
                     /gene="zwf"
                     /locus_tag="OQM34_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1452509.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14580..15449
                     /gene="hexR"
                     /locus_tag="OQM34_002062"
                     /gene_synonym="yebK"
     CDS             14580..15449
                     /gene="hexR"
                     /locus_tag="OQM34_002062"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1452510.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15577..17019
                     /gene="pyk"
                     /locus_tag="OQM34_002063"
     CDS             15577..17019
                     /gene="pyk"
                     /locus_tag="OQM34_002063"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1452511.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17150..18121)
                     /gene="lpxM"
                     /locus_tag="OQM34_002064"
                     /gene_synonym="msbB"
     CDS             complement(17150..18121)
                     /gene="lpxM"
                     /locus_tag="OQM34_002064"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1452512.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18241..19563)
                     /gene="mepM"
                     /locus_tag="OQM34_002065"
     CDS             complement(18241..19563)
                     /gene="mepM"
                     /locus_tag="OQM34_002065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1452513.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(19579..20511)
                     /gene="znuA"
                     /locus_tag="OQM34_002066"
     CDS             complement(19579..20511)
                     /gene="znuA"
                     /locus_tag="OQM34_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1452514.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            20590..21345
                     /gene="znuC"
                     /locus_tag="OQM34_002067"
     CDS             20590..21345
                     /gene="znuC"
                     /locus_tag="OQM34_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1452515.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21342..22127
                     /gene="znuB"
                     /locus_tag="OQM34_002068"
     CDS             21342..22127
                     /gene="znuB"
                     /locus_tag="OQM34_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1452516.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGMVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22274..23284)
                     /gene="ruvB"
                     /locus_tag="OQM34_002069"
     CDS             complement(22274..23284)
                     /gene="ruvB"
                     /locus_tag="OQM34_002069"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1452517.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23293..23904)
                     /gene="ruvA"
                     /locus_tag="OQM34_002070"
     CDS             complement(23293..23904)
                     /gene="ruvA"
                     /locus_tag="OQM34_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS1452518.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24043..24108)
                     /gene="yobI"
                     /locus_tag="OQM34_002071"
     CDS             complement(24043..24108)
                     /gene="yobI"
                     /locus_tag="OQM34_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS1452519.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24178..24780
                     /locus_tag="OQM34_002072"
     CDS             24178..24780
                     /locus_tag="OQM34_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCS1452520.1"
                     /translation="MNINYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(24782..25303)
                     /gene="ruvC"
                     /locus_tag="OQM34_002073"
     CDS             complement(24782..25303)
                     /gene="ruvC"
                     /locus_tag="OQM34_002073"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1452521.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25338..26078)
                     /locus_tag="OQM34_002074"
     CDS             complement(25338..26078)
                     /locus_tag="OQM34_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1452522.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26107..26559)
                     /gene="nudB"
                     /locus_tag="OQM34_002075"
     CDS             complement(26107..26559)
                     /gene="nudB"
                     /locus_tag="OQM34_002075"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1452523.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPVAAALTKSWSNRQAIEQFVINAA"
     gene            complement(26677..28449)
                     /gene="aspS"
                     /locus_tag="OQM34_002076"
     CDS             complement(26677..28449)
                     /gene="aspS"
                     /locus_tag="OQM34_002076"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1452524.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            28759..>28961
                     /locus_tag="OQM34_002077"
     CDS             28759..>28961
                     /locus_tag="OQM34_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCS1452525.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALK"
BASE COUNT         7043 a         7467 c         7126 g         7325 t
ORIGIN      
        1 ctcctgctgc cctaattgaa aagcttaaag gggtcgactc atttactatg gcaggctgga
       61 ttgaacgtta caaaacgatt cttataagga gaaaagcgtc cagaaatact tataaaactc
      121 gggtaaatca actggagaca ataaaagaaa aattaggagg ggttttactg acagaaataa
      181 ccactcgcca tatcgccgag tttcttgatt tgtagattga aggagggaaa aacacgatgg
      241 caggatcaat gcgttctgtg ttatctgata tgttccgcga agccattgtt gaaggacgta
      301 tatctcaaaa tccagtaacg ccaacaagag caccgaaaat agtagttaca agagaacggc
      361 tgaaactaaa gacatacaac tgcatcaggg aggcagcaga tcaacttccg gcatggttcc
      421 cattagctat ggacttagcc cttgtaacag gacaacgtcg cgaagacata acgaatatgc
      481 ggttcagtga tatttatgat gatcgtctcc acatcaggca aattaagaca ggaatgatga
      541 ttgctatccc cctgtcactc agccttcctg tcgctggttt acgacttggt gcagtagttg
      601 aacagtgccg catggtaagt aggggggatt atctaatcag tgccgggatt agaaaaaaca
      661 gccctgacgg cagcattcac ccggacggcc tgacaaagaa atttgtcgca gccagaaaat
      721 taacaggtat cctgttcagt gaaaacccac caacttttca cgaaatcaga agcctggctg
      781 gacgattgta caaagaaata tgtggagaag aatttgctca gcgtctactt ggccacacat
      841 cggagaagac aacaaaaatg tatcttgatg agagagaaaa aacgtactta ctgctctgat
      901 tttaacgtaa atggattgtt aaatgtattt tggttgtgat ataaccaaaa aagaccggaa
      961 tacagaaatt cgagtaaatt tcggggaatt tcggggagac gtttgcaact aattgatttt
     1021 aaatacaatt aaaaaaagac cgaatacgat tcctgtattc ggtccaggga aatggctctt
     1081 gggagagagc cgtgcgctaa aagttggcat taatgcaggc ttagttgcct tgccctttaa
     1141 gaatagatga cgacgccagg ttttccagtt tgcgtgcaaa atggtcaata aaaagcgcgg
     1201 tgctcatcag cttaaatgtt aaaaaccgcc cgttctggtg aaagaactga ggcggttttt
     1261 ttattggaaa tcaaaaggct attttaggta attaacagag tttttcagct cgttctataa
     1321 acggtgccag actcattttt tcgccgggat tgttaggatc atcaatctga atcaccgaaa
     1381 tgggttgggc attggtcttc ccactggcaa cttccttttg tgcgatatcg tttaaaggat
     1441 actgcacgag ggtacttggg ttgatgacat acaaagcatt acccggtcgg caagtcagca
     1501 ttacctcttc gcgattaaac gcccatttgt ctttacccac ttcaaaacga ctcacggtta
     1561 tcacctgtgg agccgccagc gccgctgcag aactggtgag taacagaaac gccagaatac
     1621 tttttttcat cattttcgta atccatcaaa aaggttcgag agtcgcaagc aggctgatga
     1681 tcaccagcac taccgcacct atcgcccatt cgagtttagt catccacaca aaccagggtg
     1741 ccgcacgatc ttcatcctgc ctcatgcgcg gtacaagaac ataacgatta gccagcgcaa
     1801 tgaccaccat aatcatcacc aggatggctt tgagcaataa gagttggccc cagtaagtcg
     1861 taagcgtggg gggaaatccg gtaatcaaca atgcattaag cacgccgctt gccagtacgc
     1921 cgatcaccgc aaaatgcccg catcaggaaa aacgcatcag cgcctgaata gcctagtgtc
     1981 gccaacggcc tttgatgagt tgcatacacc agagcaccgg cagtaatccc ccaaaccacg
     2041 ccgccgcaca aatcaggtga atcgcgtgat tagtctgatg gattttcgct gttacccctt
     2101 cattcagcgt cgcatgcccc actcccgcca gcagaataaa ttgcgcggta gtgagcataa
     2161 acagcaaacg tggcatattc cgcggttgca taagggcgac gatcaacgtt acgagggcga
     2221 gaacaatttg ccataaccag acgccaccaa actgcgtttg caaaacagcc tgccaaatgt
     2281 ttggcgaaaa tacatctgtc catcccgttc ccatcaaccc gccctgaacg gcgagcattg
     2341 cagtggcact gatcaaactc cagacggcag cgtgctgttg taaacgcaga gaacgcttcg
     2401 tcagcaagcg acgaatcatt aagggggcca gccaggcacc atacattgca aagccaaaga
     2461 ccagcatcaa cgaggtgaaa tggataaatc gcagcgcgat ccaggtaaac gccagcatga
     2521 tttatttcac gctaaaggtg tagtgtcctt tcgttttatg cccgtccacc gaaacaacat
     2581 gccagtcaac ggtgtaggtt ccgggtttca gcgaatcggc cagtggaaca atcagttgct
     2641 tttggtcctg ctcattccgc ttcgctggta atgttttaat attttcgttt tttggccccg
     2701 tgatttttgc accactgaat cctgtttcaa cgccttccga gaagtttaaa gtgattgcct
     2761 gcggggcagc tgtcacttgc gcgtttgccg caggatactg atgcgttaaa tgcgcatgtg
     2821 cccagacgga aggtgtcacc aacgaagtgg tcaggattgc cagcgcgtag cgaagggagc
     2881 gtgcagttga agccatatta tctattcctt tttgtaataa cttttttaca gagcataacc
     2941 ttgtctaatg tctgagtcga ggatcatcaa ttccggcttg ccatccaggc tcactcttag
     3001 taacttttgc ccgcgaatga tgaggagatt aagaatgctg aagaatctgg ctaaactgga
     3061 tcaaacagaa atggataaag tgaatgtcga tttggcggcg gccggggtgg cttttaaaga
     3121 acgctacaat atgccggtga tcgctgaagc ggttgaacgt gaacagccag aacatttgcg
     3181 cagctggttt cgcgagcggc ttattgccca ccgtttggct tcggtcaatc tgtcacgttt
     3241 accttacgag cccaaactta aataaaactt atacagagtt acactttctt acataacgcc
     3301 tgctaaatta tgagtatttt ctaaaccgcc cccataattt gcagtcattt tgaaaaggaa
     3361 gtcattatgt cgttctggaa agttgcagca gcgcaatatg agcctcgtaa aacctcactc
     3421 accgaacagg tagctcatca tctggagttt gtgagggccg ccgccaggca acaatgtcag
     3481 ctgttagttt ttccatcgct ttctttactg gggtgtgatt attcgcgacg tgcccttcct
     3541 gccccacccg atctttcact gttagacccg ctttgttatg ccgcaacgac ctagcgaatg
     3601 accatcattg ccggccttcc cgttgaatat aacgatcgct ttattcgtgg aattgcggtg
     3661 ttcgcaccgt ggcgaaaaac gcctgggaac taccatcaga gtcatggtgc atgtctgggc
     3721 cgtcgttcca gaaccatcac ggtggtcgat gaacaaccgc aaggtatgga tatggacccg
     3781 acctgttcac tgtttacgac agggcaatgt ctgggagaac ccgacctgct ggcgtcggcc
     3841 cgtcgcttac aatttttttc acatcagtac tcgattgccg tgctgatggc aaatgcccgt
     3901 ggtaacagtg cactatggga tgaatatggt cgtctcatcg ttcgcgccga tcgtggttca
     3961 ttattgttag tcggtcagcg ttcatcacag ggttggcaag gcgatatcat tccattacgc
     4021 taggcttttt cggcctggag catgccatgt tgcgcattat cgatacagaa acctgcggtt
     4081 tgcagggagg gatcgttgag attgcctctg ttgatgtcat tgacggaaaa atcgtcaacc
     4141 ccatgagcct cctggtgcgc cccgatcgtc ctattagtcc acaagcgatg gcgattcatc
     4201 gcatcaccga agccatggtc gccgataaac cgtggattga agatgtgatc ccacactatt
     4261 acggtagtga atggtatgtc gcgcacaacg ccagctttga ccgccgcgta ctgcctgaga
     4321 tgcccggtga gtggatttgc actatgaaac tggcccgtcg tttgtggcca gggctcaagt
     4381 acagcaatat ggcgttatat aaaacacgca agctcaatgt acagacaccg ccgggcctgc
     4441 atcatcaccg cgcgttgtat gactgttata tcaccgccgc gttgcttatc gatattatga
     4501 acacctccgg ctggacggca gaacagatgg ccgatatcac cggacgtccg tcgttgatga
     4561 cgaccttcac ctttggcaaa taccgtggca aagcggtttc cgacgttgcc gaacgctatc
     4621 cgggctatct gcgctggtta tttaataacc tggacagcat gagcccggag ctgcgtttaa
     4681 cactgaaaca ttatctggaa aatacttaat ccgcaggcgt agcgggtaat gttccctgcg
     4741 ccagcgcgac cagaaaagca tattccatcg ctacgccttc gtacgattta aagcggccag
     4801 atttaccgcc atggcctgag tccatgtcgg tacagagcaa taaaagatgg tcatcggttt
     4861 tcagctcgcg caatttagcg acccattttg ccggttccca atattgcacc tgagaatcgt
     4921 gtaaaccggt cgttaccagt aaatgcggat aagcctgtgc ggtgacgttg tcatacgggc
     4981 tgtagctttt catgtactcg taatattgcg gatcctgcgg atttccccac tcttcaaact
     5041 caccagtggt aagaggaatt gattcatcaa gcatcgttgt tacaacatca acaaacggta
     5101 cctgggcgat aacgccgtgg aataattccg ggcgttgatt aattgcaacg cccatcaaca
     5161 tgccccccgc acttccgccc atcgcataac agagcgaagg agagccatag cccagtttta
     5221 acaatgcatc gcaggcatca agataatcat taaacgtatt tttcttcttc agaaatttac
     5281 cgtcttcgta ccattgttgc cccagctcac caccgccgcg aacatggaca atggcgtaga
     5341 caaagccacg atctaacaaa ctcaagcggc taaaactgaa atcggcatca atacttgcgc
     5401 cgtaagaacc atagccatac accagcaacg ggttgtgtcc tttgcgaaaa tgtttgcgat
     5461 gatagaccag cgaaaccgga acttcgacgc catcacgggc gactatccac aggtgttcac
     5521 tgcggtaatt cgccgcatca aaaccaggca cttctgtttg ttttaataca cgacgctcac
     5581 cggtatccat atccagttca aacaaagtgt ctggtgtggt catggaagaa taaccataac
     5641 gcaatcgcgc ggtttcaggt tctggattgt aggcaatcca ggtcacatag gccggatcat
     5701 caaaggcaat gccaatgact tcccgggtct tgcggttaat ttggcgcaaa ctggttaacc
     5761 cgcgctgacg ctcttcaacc accagccagt cggtaaacag cgtaaaccct tccagcatga
     5821 tgttttcgcg tggcggaatt aactcttccc actgttgctc atcacgcata cgggtacggt
     5881 ataagccaaa gtttttgccg tggcgattgg aacgcagata aaaacgatgc tggtagtgat
     5941 caaggctgta ttcgtgatct ttgcggcgcg gcagaaaaac aaacggctcg gcatcggcca
     6001 tttccgcgtc cagcaggcga acttcactgg tggtggcgct ggccagatga atgacgacat
     6061 agtgcttcga cgtcgtttta tgcaggctga cataataggt atcgtctttt tcttcgtaga
     6121 tcagtgtatc ttgcgatgct ggcgtaccga tggcgtgacg ccagacctga taaggcagca
     6181 gcgtcaccgg atgcttgcga acatagtaga aagtccagga gtcatttgcc cagacaaagc
     6241 tgggttcaac gttatccagc agttccgggt accagttacc tgtttccaga ttacgaaaac
     6301 gaatgccgta ctggcgtcgg gaaagaaaat cttctgccag cgccataatg gtgttatcgg
     6361 gcgtaatcgc cattccgccc atcgaataaa actcactatg agctgcgcgc ttgttggcat
     6421 cgagcaatgt ttcccactca tcccactctt cgctgaatgc cgattgacgc tggtagatag
     6481 catattcaca gcctggttca taaatatgcc gatagcggta gccatttttt atgtagggcg
     6541 cagaaacttc tctttgcgga atgcggtcga tgatttcctt taagatgcga tcctgcaagg
     6601 cttgttgtga ggccatcacc cgatgaccgt aactattttc ttgttgcagg tagtccagga
     6661 cttttggctg agaacgcgta tcgtcccgca gccagtagta attatcgatg cgcgtatcgc
     6721 catgaagcgt catggcgtgg ggaatgcggg cggcttttgg tagcatgtta ttgttctttc
     6781 tggttggaaa catcttataa gggtggcaaa actcaccggg gatgcaagcg aaacagggtc
     6841 gtcattgctt agatgatgac aggtaatggc gcggatatcg aatgttatgc agacagagaa
     6901 aatcagcctg ttcaaatggc tgtgcggttc tggatagccc gaaatagtca acttcaggct
     6961 atccagagag cggaattatt ccgccaaagt gcgtttttgc tgttcgagat cgcgttcaat
     7021 gccttcacga acatcctggg ggattttcag cgcgtcaccc agtgcattca ggtaactgcg
     7081 ttccataaaa tggtcaatat caatagccgc gcagctcagg aaatagattt ccagcgcctc
     7141 ttcttcattg cggaccccgg tagccaggcg ttgtggatcc agcggttgtt cgattgcctg
     7201 ctcaatgagt acacgcccct gctcttccac gccggcttca cgcaattgct ggtcgattgc
     7261 cgcacgttca ttggcatcaa tatgaccatc acttttagcg gcaaagacca gcgcaaggat
     7321 caaacgttcc gtacgctcat ccagcggcgt actttgcgcg ccaaactgcg gttcgtcctg
     7381 atgcgccgcg cgaattttat ctttgtattt attccacagc accgtacccg ctaccgctcc
     7441 gccgccaacc agtaacgcgt tggtgccata tttcgtaaga agtttacgtg ctgatttatt
     7501 tgcgaccagc agcccagcca gtccgcctaa tgcgcctgac actaacagtt tgcccaatcc
     7561 ctgatccgca gacgaggagg tagaagaact gctttgccca agcagggatt gcagttgatt
     7621 taaccagtta gccatatttg ctcctcaata accattttta gtgcctgtag catagcggat
     7681 gaggatgtca gaaaatgtat atcgagacaa aagatgcgca aataagcggg gcggtaggcc
     7741 gttattcaaa gaaattcgcg ccaggtaagg tatttactgt atatcaagtg ataagggtat
     7801 tttaggtgaa agtagtttgt cgttttgagt ggcattgttt gatgctgatt gcccgatgcg
     7861 acgctgttgc gtcttctcag gcctacgatt gtggtgcgtt ctgggacgga caaggcactc
     7921 gcgccgcatc cgccaacggt caatcgaatt atcagaagcc attaacgccg ctgatattcc
     7981 gccattcccg ctttgcagtc cacgctgacc tggtaatgaa tatcggcact tttaccacgc
     8041 acggttagcg gtactgacca tttatcatct ttaccctgaa tgtcctgcaa actgacccag
     8101 gccacgggat cggcctgacc gacaattttt tgatcatctg cccagcgcgc cacgcgattt
     8161 tgttgataat cacgttttac gctcgcggca attccggcag catccagacc ttcacacttt
     8221 gggaaagtga ccgacttgct ggtttcatta ttggcagcga aaactgatgc gcaggcagaa
     8281 accaacaacc gccctaaaaa cgcccctctt tttttcatgt ttttctccat agcacaatga
     8341 ttcaggagaa agcatggtac aaattgtcag gagcgcaagt tgcttcaggc cgcgtgtgag
     8401 gcatcttcct cttcggattc agcaccgacg acctgtgggg acggtaattt gccggttttc
     8461 agaatggtgc tcagcacctc tttttgttct gccagccata gcgaaagggc ttcacgttgc
     8521 tcgtcttcca tttgcactgg agaatttgtc agctaggtgt caagcagatc cgccgtatca
     8581 agcattttgt cataagcatc ggcttccttt ttgctggtaa acgacatttt ctcttcgccc
     8641 tcacgaatga ctacgtattt aacttcaacc gccatttgca gcctctcata ataactgaga
     8701 ttttatacag tatatttctt ttcggttgag aaatcaacat cagcaataaa gacacacgca
     8761 aacgttttcg tttatactgc gcgcggaatt aatcagggga tattcgttat gacgttatta
     8821 ggcactgcgc tgcgtccggc agcaactcgc gtgatgttat taggctccgg tgaactgggt
     8881 aaagaagtgg caatcgagtg tcagcgtctc ggcgtagagg tgattgccgt cgatcgctat
     8941 gccgacgcac cagccatgca tgtcgcgcat cgctcccatg tcattaatat gcttgatggt
     9001 gatgcattac gccgtgtggt tgaactggaa aaaccacatt atatcgtgcc ggagatcgaa
     9061 gctattgcca ccgatatgct gatccaactt gaagaggaag gactgaatgt tgtcccctgc
     9121 gctcgcgcaa cgaaattaac gatgaatcgc gagggtatcc gtcgcctggc ggcagaagag
     9181 ctgcagctgc ccacttccac ttatcgtttt gccgatagca aaagcctttt ccgcgaggcg
     9241 gttgccgcca ttggctatcc ctgcattgta aaaccggtga tgagctcttc cggcaagggg
     9301 cagacgttta ttcgctctgc agagcaactt gctcaggcat gggaatacgc tcagcaaggc
     9361 ggtcgcgccg gagcgggccg cgtaattgtt gaaggcgtcg ttaagtttga cttcgaaatt
     9421 accctgctaa ccgtcagcgc ggtggatggc gtccatttct gtgcaccagt aggtcatcgc
     9481 caggaagatg gcgactaccg tgaatcctgg caaccacagc aaatgagccc gcttgccctt
     9541 gaacgtgcgc aggagattgc ccgtaaagtg gtgctggcac tgggcggtta tggtctgttt
     9601 ggtgtcgagc tgtttgtctg tggtgatgag gtgattttca gtgaggtctc ccctcgtcca
     9661 catgataccg ggatggtgac gttaatttct caagatctct cagagtttgc cctgcatgta
     9721 cgtgccttcc tcggacttcc tgttggcggg atccgtcagt atggtcctgc agcttctgcc
     9781 gttattctgc cacaactgac cagtcagaat gtcacgtttg ataatgtgca gaatgccgta
     9841 ggcgcagatt tgcagattcg tttatttggt aagccggaaa ttgatggcag ccgtcgtctg
     9901 ggggtggtac tggctacttc agagagtgtt gttgacgcca ttgaacgcgc gaagcacgcc
     9961 gccggacagg taaaagtaca gggttaaacc cggcaaaaaa acgctacaaa aatgcccgat
    10021 cctagatcgg gcattttgac ttttacagct tagcgccttc tacagcttca cgcgccagct
    10081 tagtaatgcg gtcgtaatcg cccgcttcca gcgcatctgc cggaaccagc caggaaccac
    10141 cgatgcacag cacgcttttc agtgccaggt agtcacggta gttagccgga gaaataccac
    10201 ccgtcgggca gaaacggacc tgggagaacg gacccgcgat cgcctgcagg gctttcacgc
    10261 cgccgttagc ttcagccggg aagaatttga actctttcaa accgtagtcc atacccagca
    10321 tcagttcgga aacagtgctg atccccggaa tcagaggaat agtcccttcg gtagcagctt
    10381 tcagcagcgg ctcggtcaga cccgggctaa ttgcgaactg tgcacccgct tcagtgactt
    10441 ctgccagctg ctgtggattc agcaccgtac cggcacccac aatcgcttca ggcacttctt
    10501 tggcgatagc acggatagcg tcaactgcac actcggtacg cagagtcact tccagaacgc
    10561 gcaccccacc agcaaccaac gcttttgcca tcggcaccgc gtgttccagt ttttttacca
    10621 cgataaccgg tacaaccggg ccggtggtca ggattgattc tgcacttgtt ttccagtttt
    10681 tcatcagagt tttctctcgc ctgattacaa atttgtcgtc ttaaaaagtg atacaggttg
    10741 cgccctgttc ggcaccggac agtttttcac gcaaggcgct gaataattca cgtcctgttc
    10801 ccacgcgtga cgcgctcagg tcaggaatgt gcggttcgcg agcagccagt tccgcttcgt
    10861 ctaccagcag tgtcagttcg cctgtctgtc cattcacacg aatgatgtcc ccgtcgcgca
    10921 cttttgccag cagcccgcca tcgtaggctt ctggtgttac gtggatagct gacggcactt
    10981 tacctgaagc gccggagagt cgtccatcgg taactaacgc aattttgaaa caccggtcca
    11041 ataatacacc aagtggcggc atgagtttat gtaattctgg cattccgttc gcttttggcc
    11101 cctgatgacg gacaacaacg acacaatcgc ggtccagcaa acccgcttca aaggccggca
    11161 taacgtcatg ctggctttca aaaacaaccg ctggcgcttc aatcacctgg ttctcaaccg
    11221 gcacggcaga ggttttcata accgcacggc ccaggttacc gcttaacact tttgtcccac
    11281 catgatgaga gaaaggttgt tcgaaggaag cgatcacatt gctgtcgagt gatttttccg
    11341 ccccttcccg ccagtccagt tcaccattat tcagccatgg ttcaagggta taacgagaca
    11401 gaccaaagcc tgccaccgta ttgacatctt catgcagcag gcctgctttg agcagttcac
    11461 gcaccagaac cggtacgcca cctgccgcct ggaagtggtt aatatcggcc ggaccgttcg
    11521 ggtagagacg tgccatcagc ggtacaacat cagaaaggtc agagaagtca tcccagttaa
    11581 tctgaatacc ggccgcgcgc gccatcgcca ccaggtgcat ggtgtggtta gtggaaccac
    11641 cggtcgccag cagtgcaacg ataccgttca ccaccacttt ctcatcgatc atcttaccga
    11701 tcggcatcca ttcattacca ttaccggtca tgcgtgtaac ctgacgcgca gcggcagcgg
    11761 tcaaagcatc gcgcagcgga gaatccggat gaacaaaaga agagcctggc aactgcatcc
    11821 ccataaattc caccaccatc tggttggtgt tggcagtacc gtagaaagta catgttcccg
    11881 gcgcatggta agacgcggct tctgactcca gtaaggccat gcggtccact ttaccttcgg
    11941 cataaagctg gcgaatacgc actttttctt tatttggcaa accgcttgcc atcggtccag
    12001 acggcacaaa caccgcaggc aaatgaccaa acgccagggc tgccatcgtc agacccggga
    12061 caatcttgtc gcacacaccg aggaacagag caccatcaaa catgttatgg gacagcccca
    12121 ccgccgcaga catcgctatc acttcgcggc ttagcagcga caattccatt ccatcctgcc
    12181 cctgggtgac accatcacac atcgccggaa caccgcccgc aacctgacca accgcattcg
    12241 cttcatgcag ggctttacga atgatttctg gatagtgttc ataaggctgg tgcgcggaga
    12301 gcatgtcgtt ataggaggtg atgatggcga tattgttacg caacatgctt ttcaaagagg
    12361 ctttgtcttc tggctggcag gcagcgaaac cgtgtgccag gttaccgcat gccaactgcg
    12421 aacgatgaac ggtcgacgtt ttcgcttgtt ctatccgggc gagataagca gagcgagtct
    12481 cgcgcgaacg ttcaatgatt cgatttgtta cgcgtaacaa ttgtggattc ataaaggctc
    12541 ctgaaattga gttgtcagag caggatgatt cacaacgcgt ttcattcaga ggatttatga
    12601 gtgaaacgcc tgtaaccgga gctcataggg caaaaacgtt tcagtcagtg taataaaaaa
    12661 agcctcgtgg gtgaatccgc acgaggcctg aaagtgtaaa aattgttcta caatctgtgc
    12721 aagatcatgt taccggtaaa ataaccataa aggataagcg ctgatattac tcaaactcat
    12781 tccaggaacg accatcacgg gtaatcatcg ccaccgaggc aacgggtccc caggttccgg
    12841 cctgatacgg tttcggcgca tcattgtcca tcgcccacgc ctcagtaatg gagtctaccc
    12901 atttccaggc ttcttccact tcgtcgcgac gtacaaacag tgcctgaata ccacgcatgg
    12961 tttccagcag caaacgttca taggcatccg ccagatgcgt ctgattaaag gtttctgaat
    13021 agctcagatc cagcttggtg atttgcaggt tatgtttgtg gtcaaggcca ggaactttat
    13081 tcagtacctg gatatccacg ccttcatcag gttgcagacg gatagtcagt ttattctgcg
    13141 gcagatcctg ccacgactct ttaaacagat tcagttcagg tgttttgaaa tagaccacga
    13201 cttcagaaca tttggtcggc agacgtttac cagtacgcag gtagaatggc acaccggccc
    13261 agcgccagtt atcaatgtcg acgcggatcg ccacgaaggt ttctgtattg ctgcttttgt
    13321 tcgcgccctc ttcttccaga tatcccggca cttttttgcc ctgggcgaag cccgcagtat
    13381 attgcccgcg tacggttttt tcgcgtacgt tggagcggtc gatgcggcgc agggacttca
    13441 gtactttcac tttttcatcg cggatgctgt ctgcgctcag gtcagatggc ggagacatcg
    13501 caatcatgca gagaatttgt agcaggtggt tctgaatcat gtcacgcatc tgaccggctt
    13561 tatcaaaata gccccagcgc ccttcgatcc ccacttcttc tgccacggta atctcaacat
    13621 gatcaatggt gcgattgtcc cagttattca caaacaggga gttagcaaaa cgcagcgcca
    13681 acaggttcag caccgtttct ttaccaagat agtggtcgat acggtaaacc tggcactcct
    13741 cgaagtattc gccaacctga tcattgattt cctgcgaggt cgccagcgac gtccccagcg
    13801 gtttctccat gactacgcgt gccggtttag cattcagttt tgcctcgcca agccctttgc
    13861 aaattgcgcc aaaagtgctg ggcggcatgg caaagtagtt aatagtgata cgattttttt
    13921 gatccagcat cgcgccgaga cggttgaatg cagcagtgtc attgacatcg agattacaaa
    13981 aatccagacg tgcactcagg gtgtcccata aaccttcatc aatggtttct ttcatgaaag
    14041 tttcgagcgc ctcgcggaca actttggtat atgccgcttt atcccagtca gcacgcccta
    14101 cgccgataat ccgggtgtcc gggttgagct gaccgacttt ttccagttga tacagggaag
    14161 gcagcaattt acgacgcgca aggtcgcctt tcgcgccgaa aatgaccagg tcacaggcct
    14221 gggctgtttg cgttaccgcc atgtcattct ccttaagtta actaacccgg tacttaagcc
    14281 agggtatact tgtaattttc ttacggtgca ctgtactgct tttacgagct tgcgaaaact
    14341 gtaaacgctt atccacccgt gcgattacgg gaaaagcgcg caaagtgcgg caaaactgat
    14401 aaaaaaatca tcgttttcca ttcggtaaaa atctgacact gatcatgtta tgaaaaaaaa
    14461 taacaacttt tttatctgct tttgtcatta acacggcaca caggcgtaat atctgacaaa
    14521 actgcatttc gatttctttc agtgcggaaa tcgtcattac ccgtgagtct ctttacatca
    14581 tgaatatgct ggaaaaaatc cagtctcagc tggaacattt gagcaaatca gagcgcaaag
    14641 ttgccgaggt cattctggct tcgcccgata acgcgatcca ttcgagtatt gctgctctgg
    14701 cgctggaagc caatgttagc gaaccgacgg tgaatcgttt ctgtcgcagc atggacacgc
    14761 gcggttttcc tgattttaaa cttcatctgg cacaaagtct ggcgaatggc actccctatg
    14821 ttaatcgcaa cgtcaatgaa gatgacagcg ttgaatcata cacggggaaa atatttgagt
    14881 ccgcaatggc aacgctcgat catgtccgtc attcactgga taaatctgcc atcaaccgcg
    14941 ccgtcgactt gctcactcag gcaaaaaaaa tcgccttttt cggattaggc tcttctgccg
    15001 ccgttgccca cgatgcgatg aataagttct ttcgttttaa tgttccggtg gtgtactccg
    15061 atgatatcgt gctgcaacgc atgagttgta tgaattgtag cgacggagac gtggtggtgc
    15121 tgatttctca cacaggaaga acaaaaaatc tggtcgagct ggcgcagctg gcacgcgaaa
    15181 acgacgccat ggtgattgcc ctcacctctg cgggtacccc gctcgcccgg gaagcaacgc
    15241 tggcaattac cctcgacgta ccggaagata ctgacattta tatgcccatg gtttctcgac
    15301 ttgcacagct gaccgtgata gatgtgctgg cgacaggatt tactttgcga cgcggtgcaa
    15361 aattcagaga taacttgaag cgggtcaagg aagcgctgaa ggaatcgcgt tttgataagc
    15421 agttacttaa tttaagtgac gatcgctaaa agcgactgtc actgtcctaa tcttatacga
    15481 catccgaatg agattaattt atcgccatcg cggcgttatt tcattcggat ttcatgttca
    15541 agcaacacct ggttgtttca gtcaacggag tattacatgt ccaaaaggct tcgcagaaca
    15601 aaaatcgtta ccacgttagg cccagcaaca gatcgcgata ataatcttga aaaagttatc
    15661 gcggcgggtg ccaacgttgt acgtatgaac ttttctcacg gctcgcctga agatcacaaa
    15721 atgcgcgcgg ataaagttcg tgagattgcc gcaaaactgg ggcgtcatgt ggctattctg
    15781 ggtgacctcc aggggcccaa aatccgtgta tccaccttta aagaaggcaa agttttcctc
    15841 aatattgggg ataaattcct gctcgacgcc aacctgggta aaggtgaagg cgacaaagaa
    15901 aaagtcggta tcgactacaa aggcctgcct gctgacgtcg tgcctggtga catcctgctg
    15961 ctggacgatg gtcgcgtcca gttaaaagta ctggaagttc agggcatgaa agtgttcacc
    16021 gaagtcaccg tcggtggtcc cctctccaac aataaaggta tcaacaaact tggcggcggt
    16081 ttgtcggctg aagcgctgac cgaaaaagac aaagcagaca ttaagactgc ggcgttgatt
    16141 ggcgtagatt acctggctgt ctccttccca cgctgtggcg aagatctgaa ctatgcccgt
    16201 cgcctggcac gcgatgcagg atgtgatgcg aaaattgttg ccaaggttga acgtgcggaa
    16261 gccgtttgca gccaggatgc aatggatgac atcatcctcg cctctgacgt ggtaatggtt
    16321 gcacgtggcg acctcggtgt ggaaattggc gacccggaac tggtcggcat tcagaaagcg
    16381 ttgatccgtc gtgcgcgtca gctaaaccga gcggtaatca cggcgaccca gatgatggag
    16441 tcaatgatta ctaacccgat gccgacgcgt gcagaagtca tggacgtagc aaacgccgtt
    16501 ctggatggta ctgacgctgt gatgctgtct gcagaaactg ccgctgggca gtatccgtca
    16561 gaaactgttg cagccatggc gcgcgtttgc ctgggtgcgg aaaaaatccc gagcatcaac
    16621 gtttctaaac accgtctgga cgttcagttc gacaatgtgg aagaagctat tgccatgtcg
    16681 gcaatgtacg cagctaacca cctgaagggc gttacggcga tcattaccat gaccgaatcg
    16741 ggtcgtaccg cgctgatgac ctcccgtatc agctctggtc taccaatttt cgccatgtcg
    16801 cgccatgaac gtacgctgaa cctgactgct ctctatcgtg gcgttacgcc ggtgcacttt
    16861 gatagcgcta atgacggcgt agcagctgcc agcgaagcgg ttaatctgct gcgcgataaa
    16921 ggttacttga tgtctggtga cctggtgatt gtcacccaag gcgacgtgat gagtaccgtg
    16981 ggttctacta ataccacgcg tattttaacg gtagagtaag tacgttgccg gatgcggcga
    17041 aaacgccaca tccggcctat agttcaatga tagttcaaca gatttcgaat attctgaagc
    17101 aaacttgaac ttatcatcag gcgaaggcct ctcctcgcga gaggcttttt tatttgatgg
    17161 gataaagatc tttgcgctta tacggctgga tttcgcccgg tttgcgagtt ttcagcaatt
    17221 ttagtatcca ggtgtattgt tctggtcgcg gaccaacaaa aatctcgact tcttcattca
    17281 tccgccgctc aatcgtatga tcatccgcct ctaacagatc atccatcggt gggcgcacct
    17341 gaatcgtcag acgatgcgtc ttgccatcat aaatcggaaa cagcggtaca acgcgcgcac
    17401 ggcatacttt catcaaacga ccaatcgcgg gcaacgtcgc tttataggtg gcaaagaaat
    17461 ccacaaattc gctgtgctct gggccatgat cctgatcggg taaataatat ccccagtacc
    17521 cctgacgtac cgactggatg aatggtttaa taccgtcatt tctcgcatgc agacgaccgc
    17581 caaagcgacg acgcaccgtg ttccagacat aatcaaaaac cgggttgccc tgattatgga
    17641 acatcgctgc cattttctgc ccttgcgagg ccatcagcat ggcaggaata tcgacggccc
    17701 aaccgtgcgg caccagaaag ataactttct cgttattacg ccgcatctct tcgatgatct
    17761 ccagcccttg ccagccaacg cgcggctgaa ttttctccgg cccgcgtatt gccaactcag
    17821 ccatcattgc catcgcttgc ggcgcggtgg caaacatctc atcaacaatc gcttcgcgtt
    17881 cagcttcact acgttctgga aagcagagcg acagattgat taacgcacga cggcgtgagc
    17941 tttttcccag tcgtccggca aaacgtccca gccgtgccag aatgggatca cggaactttg
    18001 gcggcgttaa agcgataccc gccatcgctg ctacacccag ccatgctccc cagtagcgcg
    18061 ggtggcgaaa ggatttatca aactcaggaa tgtattcgct attatttttt ttcgtttcca
    18121 tgcttttcca gtttcggata aggcaaaaat caatctggtg atagtgtagc ggcacaactt
    18181 gccccgcacc aaataaaaaa agccggtact gactgcgtac cggctgcgaa tggatgttaa
    18241 ttaatcaaac cgtagctgcg gcacaatctc tttggcctgc gccaggaatt cgcgacgatc
    18301 ggagccggtc agcccttcgg tacgcggcag ttttgccgtc agcgggttta cggcctgctg
    18361 gtttatccat acttcatagt gcagatgcgg cccagttgaa cgtccggtat taccggaaag
    18421 cgcgatacgg tcgccacgtt tcaccttctg tcccggtttc accagaatct tgcgcaagtg
    18481 catataacgc gtggtgtagc tgcgaccatg acgaatagcc acataataac ctgctgcgcc
    18541 actgcgtttg gcaaccacca cttcaccgtc acccactgaa agcactggcg taccttgtgg
    18601 catggcgaaa tcaacacctc tgtgtggtgc aacgcgacct gtcaccggat tagttcgacg
    18661 cgggttaaag ttagacgaga tacggaactg tttcgccgtc gggaatcgca agaatccttt
    18721 cgccagacca gtaccgttac gatcgtagaa tttgccatcc tcagcgcgga ttgcgtaata
    18781 atctttacct tctgaacgca aacgtacgcc cagcagctgg ctttgctcac gtttaccatc
    18841 aagcatttct cgagacatta ataccgcaaa ttcatcgcct tttttcagtt tgcggaaatc
    18901 catttgccac tgcatggctt taatcactgc gctcacttcg gcgctggtta aaccggcgtt
    18961 tctggcgctg gcaacaaagc tccctccgac ggtacctttc agcagattgt taacccactc
    19021 tccttgctgc atttcgctgg tcattttaaa accgttagcg gcagtacggt cataggttcg
    19081 ggtttcacga cgagacactt cccaggtgag gcgctgcagt tcgccgtccg cggttaatgt
    19141 ccaggagagt tgttgaccga ttttcaggtt acgcaattct ttgtcggcag cagcaagttg
    19201 ggtgatatca cccatatcaa taccatactg attgagaatg ctgcttagcg tatcgccagt
    19261 ggaaacaaca tattcatgca cgcccgcttc accggcgatt ttgtcatcca gttcgtcctg
    19321 gggaatggct tcatcttctt gtgcagcttg atcaatcggc tcactggctt caggtaagag
    19381 cgaacgaatt tcgttctgtt ccagctcaat ggttttgaca attggcgtgg catcacggtg
    19441 ataaacatag ggccgccaga cagcgacggc cagagtaaga acggtgagcg accccaacat
    19501 aacgcggtgt ggtcgcggta aattattaaa cgccagggcg acagagcggg ctatctgttg
    19561 cacgtaatca cttcctcatt aatctccttt caggcagctc gcatactggt tggctaattg
    19621 actcaggaat tctgaatagc ttgttttacc cagtttgata ttcgtcccca ggggatccaa
    19681 cgttcccata cgaacggatg tccctcgtgc gacgctctca acgaccgctg gcctgaactg
    19741 tggctcagca aaaacgcagg ttgctttttg ctcaaccaac tgtgttctta tttcatgtaa
    19801 acgctgcgcg ccaggttgaa tctcagggtt aacggtaaaa tgaccaagcg gtgtcagtcc
    19861 gaactgtttt tcgaaatagc cgtaagcatc gtgaaaaacg aaataacctt tccccttgag
    19921 cggcgcgagc tcgttaccaa cctgcgtttc ggttgaggcc aattgtgcct caaaatcctt
    19981 caggttggcg tcaagtttgg ctcgactttg cggcataagt tccactaatt ttccatggat
    20041 tgcaaccgct gtagcccgcg ctatctctgg ggaaagccaa agatgcatgt tgaaatcgcc
    20101 gtgatggtga tcttcgtcac ttttttccgc gtggtcgtga tcatcatcat cgccgtgaat
    20161 acttttcatc agtagcggtt tcacatcttc aagctgcgca atcgttacct gcttcgcttc
    20221 tggtaatttg cttaccggtt tttgcataaa cgcttccatc tccgggccaa cccaaacgac
    20281 taagtccgcg ttctgtaagc gttttacatc cgatggacgc agtgaataat catgttctga
    20341 agcgccgtca ggcagtaaaa cctccgtttc tgttacacca tcagcaatgg cagaagcgat
    20401 gaacccaacg ggtttaagcg aagcgacaac ggcggcatct gcggcctgtg ttgcacctcc
    20461 ccagagagcg gcggataatg ctgcgaaaag aagcgttttt ttatgtaaca taatgcgacc
    20521 aatcatcgta atgaatatga gaagtgtgat attataacat ttcatgacta ctgcaagact
    20581 aaaattaaca tgacaagtct ggtttccctg gaaaatgtct cggtttcttt tggccaacgc
    20641 cgcgtcctct ctgatgtgtc gctggaactt aaacctggaa aaattttgac tttacttggg
    20701 ccaaatggcg caggtaagtc gacactggta cgggtagtgc tcgggctggt aacacccgat
    20761 gaaggggtta tcaagcgcaa cggaaaactg cgcatcggct atgtaccgca gaagctgtat
    20821 ctcgacacca cgttgccact gaccgtaaac cgttttttac gcttacgccc tggtacacat
    20881 aaagaagata ttttgcctgc actgaaacgt gtccaggccg ggcacctgat taacgcaccg
    20941 atgcaaaagc tctctggtgg cgaaacgcag cgtgtactat tagcgcgagc attgttaaat
    21001 tgcccgcaat tattagtgct ggatgaaccc actcaaggcg tggatgtaaa tggccaagtg
    21061 gcgttatatg accttattga ccagttgcgt cgtgaactgg attgtggcgt tttaatggtt
    21121 tctcacgatc tgcatctggt gatggcaaaa accgatgaag tgctgtgcct gaatcaccac
    21181 atttgttgtt ccggcacacc ggaagttgtt tccctgcatc cggagtttat ttcaatgttt
    21241 ggtcctcgtg gtgctgaaca actgggtatc tatcgccatc atcataatca tcgtcacgat
    21301 ttacagggac gaattgtttt gcgtcgggga aatgatcgct catgattgaa ttattatttc
    21361 ccggttggtt agccgggatc atgctcgcct gtgccgcggg tccgctgggt tcgtttgtag
    21421 tctggcgtcg tatgtcttat ttcggtgata cgctggctca tgcctcatta cttggtgtcg
    21481 cgtttggttt gttgctggac gtgaatccat tctatgcggt gattgccgtt acgctgctgc
    21541 tggcgggcgg tctggtatgg ctggagaagc gtccacagct ggcgatcgac acgttattag
    21601 ggattatggc gcacagtgcc ctgtcgctgg gcctagtggt cgttagtctg atgtctaaca
    21661 ttcgtgttga tttgatggct tacctgttcg gtgatttact ggcagtgacg ccagaagatc
    21721 tcatctctat tgcgattggc atggtcatcg tggtggctat tttgttctgg caatggcgca
    21781 atttgctgtc gatgacgatt agcccggatc tggcgtttgt tgatggtgtg aaattacagc
    21841 aagtgaaatt gttgttgatg ctggtgacgg cattgacgat tggtgtagcg atgaaatttg
    21901 tcggtgcgtt gattattact tcgttgctga ttattcctgc agctactgcg cgtcgctttg
    21961 cccgtacgcc ggaacagatg gctggtgtcg ctgttttggt ggggatggtg gcagtaactg
    22021 gcggtttaac cttttccgcg ttttacgata cgccggcggg tccgtcggtg gtgctatgtg
    22081 cggcactgtt atttattatc agtatgatga aaaagcaggc cagctaatct atcgctgaac
    22141 acatttgtcg gatgcggcgc gagcgcctta tccgacctac ggttcgctat ctctggctgg
    22201 cctgataaga cgcgaacagt gtcgcatcag acacactgcc agtgtcggat gcggcgcgag
    22261 cgaccaatcc gacttacggc atttctggcg gcgttatgcc aaagtgattc cacgcccgcg
    22321 tcgtcgccat acgcccgcgc ggtgtacgct gcaaaaagcc ttgctgaatc aaataaggtt
    22381 ccagtacatc ttcaatggtt tcacgttctt cgccaatggc tgccgccagg ttatccagac
    22441 ctaccggccc accaaagaac ttatcgatta ccgccagcaa caatttgcgg tccatataat
    22501 cgaaaccttc agcatcgaca ttcaacatat ccagcgcctg agcagcgata tctgccgaga
    22561 tggtgccatc gtgcttcact tcggcgaaat cacgcactcg acgcagcaga cggttggcaa
    22621 tacgtggcgt accgcgagcg cgacgagcta cttcctgcgc accgtcatca ctcatctcaa
    22681 gccccataaa gcgtgcactg cgactgacga tatattgcag atccggcacc tgataaaact
    22741 ccagacgttg cacaatacca aaacggtcgc gcaacggtga tgtcagcgaa cctgcgcgcg
    22801 tggttgcgcc aatcagggta aacggcggca aatcaatttt aatggagcgt gccgccggac
    22861 cttcaccaat catgatatcc agttggtagt cttccattgc cgggtacagc acttcttcaa
    22921 caactggcga taaacggtgg atctcatcaa taaacagcac gtcatgcggt tcaaggttag
    22981 tgagcatcgc agccaaatcg cccgcctttt ccagcaccgg accagaagtc gtgcgtaaat
    23041 taacgcccat ttcattggcg acaatgttgg caagcgtagt tttacccaac cccggaggac
    23101 caaaaatcaa caaatgatcg agggcatcgc cgcgcagttt cgctgctttg atgaaaatct
    23161 ccatctgtga acgaacctgc ggctgaccaa catactcttc cagtaattta gggcgaatgg
    23221 cgcgatctgc tacatcttcc ggcaaagtgg taccggcaga aatcagacgg tctgcttcaa
    23281 tcatccttta cctcataacg cggcgcgtag ggcttcgcga attaatgttt cactgctggt
    23341 gtcagggcga gcgattttgc tcaccatgcg gcttgcttct tgtggtttat agcctagcgc
    23401 caccagcgcg gcaactgctt cttgttcagc atcgtcggtc gccgggctag caggagacgt
    23461 gagtaccagg tcggcggctg gcgtaaagag atcgccatgc aaacctttaa atcggtcttt
    23521 catttcaaca atcaagcgtt cggcggtttt tttgccaata cccggcagtt tcaccagtgc
    23581 ccccacttct tcacgctcaa cggcattaac gaactgctgc gctgacattc cggagaggat
    23641 cgccagggcc aacttcgggc cgacgccgtt ggttttgatc aactctttga acaacgtgcg
    23701 ttcttgttta ttgttaaaac cgtacagcag ttgcgcgtct tcacgcacca caaagtgggt
    23761 gaaaacgatc gcttcctgac ccgcttcagg gagttcataa aaacaggtca tcggcatatg
    23821 cacttcatag cctacgccgc ccacttcaat taacaccagc gggggttgtt tttcaatgat
    23881 gatgcctctg agtctgccta tcacatgacg ctcctgcgta atgaatcaaa gataatgctg
    23941 tatgataaaa aaatgctgga tagatatcca gcgaaggatg aagaaaactt gcgaggtgtc
    24001 ccgatgatct gaaaaatggc acagtataat ttattcaaca ggttatattg gaagcaaata
    24061 tttgaatatt acatattcag tgaagaaatg cgtaataaaa atatacattg cgactcctga
    24121 aaaataattt ttttatgcta ttacgtatat tcgtatctat ttcaatggaa tgacaacgtg
    24181 aatattaatt accctgctga atatgaaatt ggagatatcg tctttacatg tataggtgct
    24241 accttatttg gtcaaatatc agctgcatca aattgctgga gtaatcacgt cgggatcatt
    24301 atcggtcata acggtgaaga cttcctggtt gcagaaagcc gggttcccct ttcaaccatc
    24361 accacgctat ctcgttttat taaacgttcg tctaatcaac gctatgctat aaagcgatta
    24421 gacgctgggc tgacagaaca acaaaaacaa cgaattgttg aacaggttcc ctcccggcta
    24481 cgcaaacttt accacacggg ttttaaatac gaatcttcgc gccagttctg ttcaaaattt
    24541 gtctttgata tttataaaga ggcgctatgt attccggtgg gtgaaataga gacgtttgga
    24601 caactgttaa atagcaatcc gaatgcaaaa ctcactttct ggaaattctg gttcttaggt
    24661 tctattccgt gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca tccgggtttg
    24721 gtgttgattc acgcggaggg agttgaaacg cctcagccgg aactgaccga ggcggtataa
    24781 cttaacgcag tcgccctctc gtcaggttca gccgcgattc gctcatctgc atcgcattct
    24841 gactaacgtg gcagtgggtg atggcaatcg ccagcgcatc ggcggcatcc gcctgtggat
    24901 tagcgggcag tttcagcaag gtgcggacca tatgctgcac ctggcttttt tcggcactac
    24961 ccatacctac caccgtttgc tttacctgac gtgccgcgta ttcaaatacc ggcaattcct
    25021 gattcaccgc cgcaacaatc gccacgccgc gcgcctggcc cagtttcagg gctgagtcag
    25081 cgttcttcgc cataaagact tgttcaatgg cgaaataatc aggctggaac tgggtgatga
    25141 tttccgtcac gcccgcatag atgagcttca gacgagacgg taaatcatcc actttggtgc
    25201 gtatgcatcc gctacctagg taggacagtt gcctacctac ctggcggatg acgccgtagc
    25261 cggtcacgcg cgaacccggg tcaatgccga gaataatagc catcacgcgt ctccgttttg
    25321 ctgtttagca ggcatcatca gagagtcgct gcaacctcat cagagatttc accgttatgg
    25381 taaacttcct gcacgtcgtc gcaatcttcc agcatatcga tcagacgcat cagtttcggc
    25441 gcggtttctg catccatatc agctttggtg gacgggatca tggaaacttc cgcgctgtct
    25501 gctttcagac ctgccgcttc cagagcgtcg cgcactttac ccatttcttc ccatgcagtg
    25561 tagacatcaa tcgcgccgtc atcatatgtc acaacgtctt cagcacctgc ttccagagct
    25621 gcttccatga tggtgtcttc gtcgcctttc tcgaaggaga tcacgccttt tttgctgaac
    25681 agataagcta cggaaccgtc agtgccgagg ttaccgccac atttgctgaa tgcgtgacgc
    25741 acttcagcaa cggtacggtt gcggttgtca gacagacatt caatcatgat tgccgtgccg
    25801 ccaggaccgt aaccttcgta gatgatggtt tccatgtttg catcatcatc accgcccaca
    25861 ccacgtgcaa ttgcgcggtt cagtgtgtca cgggtcatgt tgttagacag tgctttatcc
    25921 accgccgcac gcagacgcgg gttagcgtcc ggatcgccac cgcccagctt agccgcggtt
    25981 accagctcac gaatgatttt agtgaagatt ttaccgcgct tagcatcctg cgcagcttta
    26041 cgatgtctgg tgttggccca tttactatga cctgccataa aaatatctcc agatagccct
    26101 gcctgttcag gcagcgttaa ttacaaactg ttcaatcgcc tgccggttgc tccaggactt
    26161 agtgagcgcc gccgcaacag gcgcatcaag ccacttgtaa gccagatgtt cagtgaaaac
    26221 gatctgccgc tcgtgcggaa gcgcaagaca gaaccatgat tccgtattac gcgtcacgcc
    26281 cggcgcatag cgatgacgta aatgtgaaaa aatttcaaac tctaccgtgc gctgacagtc
    26341 aattaaggtc agttgttcag cgacaacatc aatggtaacc tcttccttta cttcgcgcat
    26401 ggcagcttgc ggcgcggttt caccctcttc cacgctgccg gttaccgact gccagaaatc
    26461 gggatcgtca cgccgctgca acatcagcac ccgtttcgta tcttgtgcat agatgaccac
    26521 taagatcgaa acgggacgct tatacacttt atccttcacg ctgcctcttc gttgactgcc
    26581 ttcgctcatc ccattcacat agttatctat gctcatggga gttcactcag ttgccgcctt
    26641 gatgcaacgc gaatgatttc gtgtatttga gtcatatcag ttattctcag ccttcttcac
    26701 aacctgaatg ctcagctcag ccagtgcagt cgggttagca aagctcggtg cttcagtcat
    26761 cagacacgct gccgccgtgg ttttcgggaa ggcgataacg tcacggatat tgtcggtgcc
    26821 agtcagcagc atggtcagac ggtcaagacc gaatgccaga cctgcgtgcg gcggagtacc
    26881 gtatttcagg gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt cgttgatacc
    26941 cagaatacca aacaccgtct gctgcatatc accattatgg atacgtactg aaccaccgcc
    27001 cacttcgtaa ccattgatga ccatatcgta agcgttcgcc accgcatttt ccggtgcagc
    27061 tttcagttct gcagcagtca tatctttcgg tgaggtgaac ggatggtgca ttgccgtcag
    27121 gccgccttca ccgtcgtctt caaacatcgg gaagtcgata acccacagcg gtgcccattt
    27181 gctttcgtcg gtcagaccaa ggtctttacc cactttcagg agcagtgcgc ccatcccgtc
    27241 ggcaacaatt ttcttgttgt cggcaccgaa gaaaatcata tcgccatctt gcgcggcagt
    27301 acgatccagg atggcttcga tgatttctgc attaaggaac ttcgctaccg ggctgttgat
    27361 accttccaga cctttcgcgc gttcgttaac tttgatgtaa gccagacctt tcgcgccgta
    27421 gattttaacg aagttaccgt attcgtcgat ctgcttacgg gtcagcgatg cgccacccgg
    27481 aacacgtaga gcggcaacac ggcctttcgg atcgttcgcc ggccccgcaa atactgcaaa
    27541 ctcaacagat ttcagcagat cggcaacgtc ggtcagttcc atcgggttac gcagatccgg
    27601 tttatcagaa ccataacggc gttctgcttc cgcaaaggtc attaccggga aatcgcccag
    27661 atccacgccc ttcacttcca gccacaaatg acgcaccagc gcttccatca cttcacgcac
    27721 ttgcggcgcg gtcatgaaag aagtttccac atcgatctga gtaaattcag gctgacgatc
    27781 agcgcgcagg tcttcgtcac ggaagcattt aacgatctga tagtaacggt caaaaccgga
    27841 catcatcagc agctgtttga acaactgtgg ggattgcggc agtgcgtaga atttaccttt
    27901 gtgcacacga gaaggcacca ggtagtcacg cgcgccttcc ggcgtggctt tggtcagcat
    27961 cggagtttcg atgtcgagga agccgtggtc atccataaaa cggcgcacca ggctggtgat
    28021 tttagcgcgg gttttcaggc gctgcgccat ttccgggcga cgcaggtcga ggtagcggta
    28081 tttcagacgc gcttcttcgg tgttgacgtg gttagagtca agcggcagaa catctgcgcg
    28141 gttgatgata gtcagcgagg acgccagcac ttcaatttcg ccggtcgcca tatcgcggtt
    28201 aatatttttt tcgtcacgcg cacgtacggt gcccgtaacc tgaatgcaga actcattacg
    28261 cagttcagag gccagcttta acgcgtccgc acgatccgga tcgaaaaata cctgcacgat
    28321 accttcgcgg tcgcgcatat cgatgaagat caggctacca agatcacgac gacggttgac
    28381 ccaaccacac agagtcacct gctgccccac gtgggacaaa cggagctgtc cacaatattc
    28441 tgtacgcatg agatatccct taacttagct gccggcggat gccccctgct gcgcaggtga
    28501 ccaagtcgca gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct attatactgg
    28561 aaattctgcc gcaccgtaag agcctggccc gcgctggaac gcctcgttac cactttatat
    28621 cgggcctgaa atcagactct acgccagttt gctataaagg tgttgcccga actcataaaa
    28681 attaacaaaa tttgtcgttc cgccatcggc taatcgcatt aaggtgtgag gcacgatttt
    28741 gttttttcag gagtcagcat gcttgaactt aatgctaaaa ccactgcgtt ggtggtgatc
    28801 gatttacaag aaggcatctt accttttgcc ggtggtccac atactgccga tgaggtagtt
    28861 aatcgcgccg ggaagctggc ggcgaaattt cgcgccagcg gtcagcccgt gtttctggtg
    28921 cgagttggct ggtctgccga ttacgccgaa gcattaaaac a
//
LOCUS       DAJVPP010000048        28834 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVPP010000048 DAJVPP010000000
VERSION     DAJVPP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.048"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..99
                     /locus_tag="OQM34_002078"
     CDS             <1..99
                     /locus_tag="OQM34_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1452526.1"
                     /translation="EHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            121..1530
                     /locus_tag="OQM34_002079"
     CDS             121..1530
                     /locus_tag="OQM34_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1452527.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVVAGNVGGANEHSVSV"
     gene            1508..2287
                     /locus_tag="OQM34_002080"
     CDS             1508..2287
                     /locus_tag="OQM34_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1452528.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2284..3144
                     /locus_tag="OQM34_002081"
     CDS             2284..3144
                     /locus_tag="OQM34_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1452529.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3292..3867)
                     /locus_tag="OQM34_002082"
                     /pseudo
     CDS             complement(3292..3867)
                     /locus_tag="OQM34_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(3884..4144)
                     /locus_tag="OQM34_002083"
     CDS             complement(3884..4144)
                     /locus_tag="OQM34_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1452530.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4135..5406)
                     /locus_tag="OQM34_002084"
     CDS             complement(4135..5406)
                     /locus_tag="OQM34_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1452531.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5484..5846)
                     /gene="queD"
                     /locus_tag="OQM34_002085"
     CDS             complement(5484..5846)
                     /gene="queD"
                     /locus_tag="OQM34_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1452532.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6165..7964
                     /gene="cysJ"
                     /locus_tag="OQM34_002086"
     CDS             6165..7964
                     /gene="cysJ"
                     /locus_tag="OQM34_002086"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1452533.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTQAADEFLSELRVERRYQRDVY"
     gene            7964..9676
                     /gene="cysI"
                     /locus_tag="OQM34_002087"
     CDS             7964..9676
                     /gene="cysI"
                     /locus_tag="OQM34_002087"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1452534.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            9750..10484
                     /gene="cysH"
                     /locus_tag="OQM34_002088"
     CDS             9750..10484
                     /gene="cysH"
                     /locus_tag="OQM34_002088"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1452535.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            10748..10900
                     /locus_tag="OQM34_002089"
     CDS             10748..10900
                     /locus_tag="OQM34_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS1452536.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11094..12185
                     /locus_tag="OQM34_002090"
                     /pseudo
     CDS             11094..12185
                     /locus_tag="OQM34_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12331..13368)
                     /gene="iap"
                     /locus_tag="OQM34_002091"
     CDS             complement(12331..13368)
                     /gene="iap"
                     /locus_tag="OQM34_002091"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1452537.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            13620..14528
                     /gene="cysD"
                     /locus_tag="OQM34_002092"
     CDS             13620..14528
                     /gene="cysD"
                     /locus_tag="OQM34_002092"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1452538.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            14530..15957
                     /gene="cysN"
                     /locus_tag="OQM34_002093"
     CDS             14530..15957
                     /gene="cysN"
                     /locus_tag="OQM34_002093"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1452539.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            15957..16562
                     /gene="cysC"
                     /locus_tag="OQM34_002094"
     CDS             15957..16562
                     /gene="cysC"
                     /locus_tag="OQM34_002094"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1452540.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            16612..16935
                     /locus_tag="OQM34_002095"
     CDS             16612..16935
                     /locus_tag="OQM34_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1452541.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17129..17440
                     /gene="ftsB"
                     /locus_tag="OQM34_002096"
     CDS             17129..17440
                     /gene="ftsB"
                     /locus_tag="OQM34_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1452542.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17459..18169
                     /gene="ispD"
                     /locus_tag="OQM34_002097"
     CDS             17459..18169
                     /gene="ispD"
                     /locus_tag="OQM34_002097"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1452543.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18169..18648
                     /gene="ispF"
                     /locus_tag="OQM34_002098"
     CDS             18169..18648
                     /gene="ispF"
                     /locus_tag="OQM34_002098"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1452544.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            18645..19694
                     /gene="truD"
                     /locus_tag="OQM34_002099"
     CDS             18645..19694
                     /gene="truD"
                     /locus_tag="OQM34_002099"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1452545.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            19675..20436
                     /gene="surE"
                     /locus_tag="OQM34_002100"
     CDS             19675..20436
                     /gene="surE"
                     /locus_tag="OQM34_002100"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1452546.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20430..21056
                     /gene="pcm"
                     /locus_tag="OQM34_002101"
     CDS             20430..21056
                     /gene="pcm"
                     /locus_tag="OQM34_002101"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1452547.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21196..22335
                     /gene="nlpD"
                     /locus_tag="OQM34_002102"
     CDS             21196..22335
                     /gene="nlpD"
                     /locus_tag="OQM34_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1452548.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22398..23390
                     /gene="rpoS"
                     /locus_tag="OQM34_002103"
     CDS             22398..23390
                     /gene="rpoS"
                     /locus_tag="OQM34_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1452549.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(23510..23917)
                     /locus_tag="OQM34_002104"
     CDS             complement(23510..23917)
                     /locus_tag="OQM34_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1452550.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24064..24657
                     /locus_tag="OQM34_002105"
     CDS             24064..24657
                     /locus_tag="OQM34_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCS1452551.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            24657..25604
                     /locus_tag="OQM34_002106"
                     /pseudo
     CDS             24657..25604
                     /locus_tag="OQM34_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(25877..26440)
                     /locus_tag="OQM34_002107"
     CDS             complement(25877..26440)
                     /locus_tag="OQM34_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452552.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            26605..26856
                     /locus_tag="OQM34_002108"
     CDS             26605..26856
                     /locus_tag="OQM34_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1452553.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            26858..>28834
                     /locus_tag="OQM34_002109"
     CDS             26858..>28834
                     /locus_tag="OQM34_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1452554.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGN"
BASE COUNT         7018 a         7148 c         7820 g         6848 t
ORIGIN      
        1 gaacacggca gcgcgtgggc gttgctggaa gggctgaaaa agcagttcga tcctaacggc
       61 attatgaata cgggtactat ctatccgatt gaaaaataat gtatcaggca gcgttccgcg
      121 atgacggggc gttgcctttt cggcttctca ggcgagaagc cgttcttatt accggacaat
      181 gaaggggtaa agatgaacac ttcaccggtg cgaatggatg atttaccgct taaccgtttt
      241 cactgtcgaa ttgctacgct cactttcggc gcacacctga ccgacggtta tgttctaggc
      301 gtcattggtt acgccattat tcaacttacg cccgccatgc aactgacgcc gtttatggcg
      361 ggaatgatcg gcggctcggc gctccttggt ttgtttcttg gcagcctggt tcttgggtgg
      421 atctccgacc atattggtcg gcaaaaaatc ttcaccttca gctttttgct gattacgctc
      481 gcttcgttct tgcaattttt tgccaccacg ccagagcatc ttattgggct gcgcattttg
      541 atcggcattg gtctgggagg cgattactca gtaggtcaca ccttgctggc tgaattttcc
      601 ccgcgccgcc atcgcggtat tttgctgggc gcattcagcg tggtgtggac cgtaggctat
      661 gtgttggcaa gtattgccgg acatcacttt atttccgaaa acccggaggc ctggcgctgg
      721 ttgctggcat cggcagctct gcccgcgttg ttgattacgt tattacgctg gggaacgcca
      781 gaatcgccac gttggctact gtgccagggg cgttttgcag aagctcacgc tatcgtgcat
      841 cgctattttg gtccccatgt tttactgggc gatgaagtgg taacggcgac ccataaacac
      901 atcaaaacct tgttctcttc gcgttactgg cggcgcacgg cgtttaacag cgtcttcttt
      961 gtctgcctcg taatcccatg gtttgtgatt tatacctggc tgccaactat cgcccagact
     1021 attggtctgg aagatgcgct gactgccagc ctgatgctta atgcgttgtt aattgtgggc
     1081 gcgctgctgg gattagttct gacgcacctg ctggcacatc gcaaattttt gctgggaagt
     1141 tttttgctgc tggcggcaac gctggtagta atggcctgtt tgccttccgg cagttcatta
     1201 acgctgctgc tttttgttct cttcagcacc accatttcgg cagtcagtaa tctggtgggc
     1261 attttgcctg cggaaagttt tcctactgac attcgctcgc tgggcgtcgg ttttgccacc
     1321 gccatgagtc gacttggcgc ggcggtaagt actggcctgc tgccgtgggt gctggcgcag
     1381 tggggaatgc aagtcacctt attgctccta gcgacagtgt tgttggttgg ttttgttgtg
     1441 acctggctat gggcaccaga aactaaagcc ctcccgctgg tggtggcggg aaatgtagga
     1501 ggtgcgaatg aacattctgt tagcgtttaa agccgaaccg gatgccggaa tgctggcgga
     1561 aaaagagtgg caggcggcgg ctcagggtaa tagcggaccg gatgtttcgc tactgcgaag
     1621 tttactcggt gctgatgaac aggccgctgc cgcgctgctg ttagcgcaga gaaaaaacgg
     1681 tacgccgatg tctttaaccg cgttgagcat gggggatgaa cgggcgttgc actggctgcg
     1741 ctatctcatg gctcttgggt ttgaggaagc tgtattgctg gagacggcgg cagatctgcg
     1801 ctttgcgccg gaatttgtcg ctcgccatat tgccgaatgg cagcgtcaga atccgctgga
     1861 tctgataatt accggctgcc aaagcagcga agggcagaat gggcaaacgc catttttgct
     1921 ggcggaaatg ctgggctggc cctgcttcac ccaggtggag cgtttcactc tcgacgcgct
     1981 gtttatcacc ctcgaacagc gtactgaaca tgggttgcgc tgttgccggg ttcgcttgcc
     2041 tgcggtgatt gccgtgcgtc agtgcggtga agtggccttg ccagtaccgg gaatgcgcca
     2101 gcgaatggct gcggggaaag cagaaattat ccgtaaaact gttgctgccg aaatgccagc
     2161 gatgcagtgt ctgcaactgg caagagcaga gcaacgacgc ggagcgacgc tgattgatgg
     2221 gcaaacggta gcagaaaaag ctcaaaagct atggcaggac tatttacgcc aaaggatgca
     2281 gccatgaata tcgcaattgt gacaataaat caggagaatg ccgcaatagc gagctggctg
     2341 gcggcgcagg atttcagcgg ttgtacgctg gcgcactggc aaatagaacc tcagcccgta
     2401 gtggcggaac aggtgctgga tgcgatggtg gaacagtggc agcgaacgcc tgcggacgtt
     2461 gttctctttc cgccgggaac gtttggcgat gaactgtcga cccggctggc ctggcgttta
     2521 catggtgcaa gcatctgtca ggtgacttcg ttagatattc caaccgtaag catgcgcaaa
     2581 tcccactggg gaaatgcgtt aacggcgacg ctgcaaacag aaaaacgccc gttatgcctt
     2641 tccctggcgc gtcaggcggg agcagcgaaa aacgccacgt tgccctctgg aatgcagcaa
     2701 ctgaacattg tcccaggtgc gcttcccgac tggctggtta gcacagagga tctgaaaaat
     2761 gttagccgcg acccattagc tgaggccaga cgggtgctgg tggtgggaca gggcggagag
     2821 gcagataacc aggaaatcgt tatgctggca gaaaagctgg gagccgaagt gggctacagt
     2881 cgggcgcgag tgatgaacgg tggcgttgat gctgaaaaag tgattggtat ttccggacat
     2941 ctgctggcac ctgaggtgtg cattgttgtt ggcgcttccg gcgcagcagc gttaatggcg
     3001 ggcgtacgca atagcaagtt tatggtggcg attaatcatg acgccagcgc agcggtattt
     3061 tcacaggcag atattggcgt ggttgatgac tggaaagtgg tgctggaggc actggtgaca
     3121 aacattcacg cagaatacca ataacgtgct gccggatgat cctgcctacg gttctggact
     3181 ggggacctgt aggcctgata agacgcgtta agcgtcgcat caggcgctga atgtcggatg
     3241 tggcgtgcgg ccaacaaatc aaaacaaatt caattggtta cgattatccc gtcaaagcaa
     3301 ttttttcgct aacgtccaga ctccggtatt cgtggtggaa agcgccacga cacccgcgtt
     3361 aatcgcatta cgcgcatctt cttcatcgca taccagcccg ccagcaatca gcggttggcg
     3421 gattttttcc gtcacccagc ccagcacttt gggcatgcag cctggcagga tctcaataca
     3481 atccggattc gattgcgcaa cttacttatc aatgttgtga aacgaaatcg aatcaacaat
     3541 aaacaggtga tgaatacaga aaaaaacttc cgctcttgcc gcttttagca tcgatgcttt
     3601 ggtgctgata attccatccg cctcggtcac cagtttcaaa aactgaatca ccacttcttt
     3661 atttgacgcg ccttccagca aatcaacatg aataaaagca tatttcccgg cgttcttaat
     3721 ctttttaacg atattactga tggtgcagat attgccgtac aacacggaaa taaactggca
     3781 ttcagaatcg atggccagtt gcaggctggc attatcttta acggcagcaa tcaccggatt
     3841 ctggcggagc aggtgtaaga ggggcataaa ttatccttat tatttatcca aagcggtagg
     3901 tgatgccgaa tccggaaggc ggatagcgcc actgttgtag tgtggattcg tcgcacagca
     3961 aacggcaggt gccacactcc aggcagccgc gatagtcaat acgtaagtca ccttcctgtg
     4021 tgagagaaaa aagacctacc ggacaggcgt taatcaaccg ttgtaccatc tgacgctcaa
     4081 cggagtcagc cggaacaatg tgcggcgcat cagcaacgcg ccagagatta cgggctacag
     4141 acatcgcaga ctcctgataa catcgcccgc cagatgccac aggccatgac gacgtaaatg
     4201 atgccagaat aactggcgca gcggtggaac aggtttatca ccctgatccc ataaatcgcg
     4261 ggaaatatcc tgcattaacg caggccacgc ccgataccag ccagggcgtt gcaaaagcgc
     4321 cggaacatgc tgataacgtt gtagaacatc ccatagcagg ctgcgctcta cgttgtgatg
     4381 ataaagcgca aacagatttt gcggctcgcg gtgctggcag gcgcttatta gcgtttgtgc
     4441 cgccgcctgt gcgccagtca gcgccatatc catgccgcgc acggaaattc cggtattgac
     4501 gcaactgcgc aacgcatcgc ccaccagcag ccagccgtta ccggcgtatt gcaccggcat
     4561 actgtgcaag ccaccttctg gcaccagatg cgcaccatac tccagtgatt ccgtgttttt
     4621 gataagcgtg cgcactgccg gatgcgcttt aaagcgagtc agcagctcgc ttgccggaac
     4681 acgactttgc gtaagggaag agagcgggca aacaatccct aacgagagcg tttgttgatt
     4741 agtataaaga aatgcgccgc cgggtaagtc atcacagatt ccgccgctga acagcaacgc
     4801 tgcgccttcg ttattctcca gatgaaaacg ttcttcaata gcggatgttt ccagcgacag
     4861 cacttctttg atccccaacg ccatcgcttc gccagcagga cgagtcacta acccgtgacg
     4921 ttcagccagg acgctgttgg caccttctgc cagcaccaca taacgggcgc ggagaatatc
     4981 gtcaccacaa ataacgccac agactctgcc gttttcttca tacagtgcat ccaccgtcgc
     5041 tccggggatg cattcgacac cttctttttc ggcttcggca accagccacg gatcgaaccg
     5101 tgcacgtaat acactccagg attcaccgcc gggctgtaag ctggaaaacg tcgttgcgcc
     5161 atccggcgtt aacagggaaa ggctttcgtg agtgatgcgt cgttcaagag gcgcggtcag
     5221 atgaaattgc gggaggagtt ccgcgagtgc atgggtatat aaccgcctgc cggaaagatt
     5281 tttgctgccg gggatttcag cgcgttcgag taacaaaacg gataaatccg ctcgcgcgca
     5341 gcgtaacgcg caagcggtgc ctgcaatacc ggcaccaata ataataatgt cgcagtcgtc
     5401 ttccatgtca tgccccagat atccagaaca caacacccta acatagcgtt actaaaggga
     5461 aattgaccgc cgacacactc ttatcattcg ccgcgataga tacaccctgc ggtgcaggtt
     5521 tcttttacca tcaccgcact taacagcggc acaacgggtt taacctgatc ccaaatccat
     5581 tttgctaaaa cttcgctggt tggattttcc agacctggaa tatcattgag ataatggtga
     5641 tcgaggcgct cataagttgg cttaaacgcc gctttcagtt cagcgaaatc gataatccag
     5701 cccgtatgcg gatcgacttc cccggtaatt tccagtcgca ccataaagga atgcccgtgc
     5761 aggcgaccac atttatgccc ttccgggaca tgtggtaagc ggtgagcggc ttcgaaggtg
     5821 aaatctttaa ataacgtggt ggacatcata atttctctac aggacgataa aaccgccgta
     5881 gagtaccgga aaggctgttt tttcgcatta tctaaaacga ctttgttgcg caaaatcgct
     5941 gatttatctt aatgattggc taaattcatt tgtttttcat taggttggtt aatctatttt
     6001 gttgttaaag actattgcta aaacaggtta gtcgatttgg ttattagtta tcgctatccc
     6061 gtctttaatc cacaccgttc accccgttaa ccttaccttc tcttctgttt tatgggcgct
     6121 gacagggcgc agaaacagct ttgcttactg gaacataacg acgcatgacg acacaggtcc
     6181 caccttccgc gttgcttccg ttgaacccgg agcaactggc acgccttcag gcggccacga
     6241 ccgatttaac gcctacccag cttgcctggg tttctggcta tttctggggc gtgctcaacc
     6301 agcagcctgt tgcgcttgca gtgacgccag cgcccgccgc agaaatgccg ggtataacga
     6361 ttatctctgc ttcgcaaacc ggcaatgcgc gccgggttgc tgaagcatta cgcgatgatt
     6421 tattagcagc aaaactgaac gttaagctgg tgaacgcggg cgactataaa ttcaaacaaa
     6481 tcgccagcga aaaactgctc atcgtagtga cgtcaacgca aggggaaggg gaaccgccgg
     6541 aagaagccgt cgcgctgcat aagttcctgt tctccaaaaa agcgccaaag ctggaaaaca
     6601 ccgcgtttgc cgtgtttagc ctcggcgata gctcttatga atttttctgc cagtccggga
     6661 aagattttga cagcaagctg gcggaactgg ggggggaacg cctgctcgac cgtgtcgatt
     6721 ctgatgttga ataccaggct gctgccagcg agtggcgcgc ccgcgtggtt gatgcgctta
     6781 aatcgcgtgc gcctgtcgcg gcaccttcgc aatccgtcgc tactggcgcg gtaaatgaaa
     6841 tccacaccag cccgtacagc aaagacgcgc cgctggtggc tagcctctct gttaaccaga
     6901 aaattactgg gcgtaactct gaaaaagacg ttcgccatat cgaaattgac ttaggcgact
     6961 cgggcctgcg ttaccagccg ggtgacgcgc tgggcgtctg gtatcagaac gatccggcac
     7021 tggtgaaaga acttgtcgaa ctgctgtggc tgacaggcga tgaacctgtc actgtcgagg
     7081 gcaaaacgtt gcctctgaac gaagcgctac agtggcactt cgaactgacc gtcaacaccg
     7141 ccaacattgt tgagaattac gccacgctta cccgcagtga aacactgctg ccgctggtgg
     7201 gcgataaagc gaagttacag cattacgccg cgacgacgct gattgttgac atggtgcgtt
     7261 tctccccggc acagcttgat gccgaagcgc taattaatct gctgcgcccg ctgaccccgc
     7321 gtctctattc cattgcctcc tcgcaggcgg aagtcgagaa cgaagtacac gtcaccgttg
     7381 gtgtggtgcg ttacgacgtg gaaggccgcg cccgtgccgg tggtgcctcc agcttcctcg
     7441 ctgaccgcgt ggaagaagag ggcgaagtcc gcgtatttat cgaacataac gataacttcc
     7501 gcctgcccgc taacctggaa accccggtga ttatgattgg cccaggcacc ggtattgcgc
     7561 cgttccgcgc ctttatgcag caacgcgccg ctgacgaagc gccaggtaaa aactggctgt
     7621 tctttggcaa tccgcatttt acggaagact tcctgtatca ggtggagtgg cagcgctacg
     7681 tcaaagaggg tgtgctgacg cggatcgatc tcgcctggtc gcgcgaccaa aaagaaaaag
     7741 tttacgtaca agataaactg cgcgaacagg gcgcggagct gtggcgctgg atcaatgacg
     7801 gtgcccacat ttatgtctgc ggcgacgcta atcgcatggc gaaagacgtt gagcaggcac
     7861 ttctggaagt gattgccgaa tttggtggca tggacaccca agcggcggat gaatttttaa
     7921 gtgagctgcg tgtagagcgc cgttatcagc gagatgtcta ctaatgagcg aaaaacatcc
     7981 agggccttta gtggtcgaag gaaaactgac agacgccgag cgcatgaagc ttgaaagcaa
     8041 ctacctgcgc ggcaccattg cggaagattt aaacgacggt ctgaccggcg gctttaaggg
     8101 cgataacttc ctgctgatcc gcttccacgg catgtatcag caggatgacc gcgacatccg
     8161 cgccgaacgt gctgaacaga agctggagcc gcgccacgcg atgctgttgc gctgccgtct
     8221 gccgggtgga gttatcacca ccaaacagtg gcaggcgatc gataaatttg ccggtgaaaa
     8281 caccatctat ggcagcattc gcctgaccaa ccgccagacg tttcagttcc acggcattct
     8341 gaaaaagaac gtcaaaccgg tgcaccagat gctgcactcg gtcggtcttg atgcgctggc
     8401 gactgctaac gacatgaacc gtaacgtact ctgcacctcg aacccttacg agtcgcagct
     8461 gcacgcagaa gcgtacgagt gggcgaagaa gatttctgaa catctgctgc cgcgtacccg
     8521 cgcgtatgcg gagatctggc tcgatcagga aaaagtcgcc accaccgatg aagaaccgat
     8581 ccttggtcag acttacctgc cgcgtaagtt caaaaccacg gtagtgatcc cgccgcagaa
     8641 cgatattgat ctgcacgcca acgacatgaa cttcgtggcg atcgcggaaa acggcaagct
     8701 ggtgggcttc aacctgctgg tgggcggtgg gctttctatc gaacacggta acaagaaaac
     8761 ctacgcccgc acggcgagtg agtttggcta tctgccgcta gagcatacgc tagcggtggc
     8821 ggaagccgtc gtgacaactc agcgtgactg gggtaaccga accgatcgta aaaatgccaa
     8881 aaccaaatac acactggagc gcgtgggggt tgagacgttt aaagcggaag tggagcgtcg
     8941 cgcggggatc aaatttgaac cgatccgccc gtatgaattt acaggacgcg gcgatcgtat
     9001 tggctgggtt aagggcattg atgataactg gcacctgacg ctgtttatcg aaaatggtcg
     9061 catccttgat tatccggggc gtccgctgaa aactggcctg ctggagatcg cgaagatcca
     9121 caaaggtgat ttccgcatta cggcgaacca gaatctgatc atcgccggtg tgccggaaag
     9181 cgagaaagcg aagatcgaga aaattgccaa agagagcggg ttaatgaatg ccgtcacgcc
     9241 gcagcgtgaa aactcgatgg cctgcgtgtc attcccgact tgcccgctgg cgatggcgga
     9301 agcggagcgt ttcctgccgt cttttatcga caacatcgat aatttaatgg tgaaacatgg
     9361 tgtcagcgat gagcatatcg tgatgcgtgt aacaggctgc ccgaacggtt gtggtcgcgc
     9421 gatgctggcg gaagtgggct tggtgggtaa agcaccgggt cgctacaacc tgcatcttgg
     9481 cggcaaccgc attgggacac gtatcccacg gatgtataaa gaaaacatca ccgagccgga
     9541 aatcctggcg tcgcttgatg aactgatagg gcgctgggcg aaagagcgcg aagcgggtga
     9601 aggcttcggc gactttacgg tgcgtgcggg catcattcgc ccggtgctcg atccggcgcg
     9661 cgatttgtgg gattaaccat cagcccggtc ttgtaggcct gataagacgc gtgagcgtcg
     9721 catcaggcaa ggcaaacagt gaggaatcta tgtccaaact cgatctaaac gccctgaacg
     9781 aactgccgaa ggtagatcgc attctggcgc tggcagaaac taacgccgaa ctggaaaaac
     9841 tggacgctga aggccgcgtt gcctgggcgc tggataatct gcccggtgaa tatgtacttt
     9901 cttccagctt tggcattcag gcggcggtga gcctgcatct ggtgaatcag attcacccgg
     9961 atattccggt gatcctcacc gacaccggct atctgttccc ggaaacctac cgctttattg
    10021 acgagttaac ggacaaactc aagctcaacc tgaaagtgta ccgcgctacc gaaagcgccg
    10081 cctggcagga agcacgctac ggcaaactgt gggaacaggg cgttgaaggc attgaaaagt
    10141 acaatgacat caacaaagtc gaaccgatga accgggcgct gaaagaactg aatgcgcaaa
    10201 cgtggtttgc tggcctgcgg cgtgaacaat ccggcagccg tgccaattta ccggtgctgg
    10261 caattcagcg tggcgtattt aaagtgctgc cgattatcga ctgggataac cgaactattt
    10321 atcagtacct gcaaaaacat ggcctgaaat atcacccatt atgggacgaa ggatatttat
    10381 cggtgggcga tacccataca acccgtaaat gggaacccgg catggcggaa gaagaaacgc
    10441 gtttctttgg cttaaaaagg gaatgtggat tacacgaagg gtaaatattg ccggataaat
    10501 taaacgcctc tgtcagaaat gatgggggcg tttttatttt gcgagcagca ttccggcatt
    10561 taaaggtgaa catcgcaggg aaaattaaca ggtatagaat ggcgttgggt gcttgaggct
    10621 gtctggcgtt aagcattcgc gaggttccag atggacaaaa gccccaggcg atatttctat
    10681 caacctgagg ctcgcgttcg aacgtagcaa ttcggatgtt agtctcttct ttgcagggag
    10741 gcaagacatg ctgacaaaat atgcccttgt ggcaatcatc gtactgtgtt gtacagtact
    10801 gggattcacg ttgatggtag gtgactcgtt atgtgagttg agtatcagag aacgtggtat
    10861 ggagtttaag gcagttctcg cttacgaatc gaagaagtag ccaccacgcg ggggtaaaac
    10921 cccgcgtact gaattgtgat ggtttggtct cctgcaccct ttttttaaat ttatattttt
    10981 tatgtgaata tatagtctct atttattatt atgaatttca aatgaaaatc attaatttat
    11041 tcataatgta aatgttttat tggctgatat atatcataga tagtttttaa aatatgcgta
    11101 aatatccttt aagtttactg aaggataaaa atattgtgac tttctttgat ttctggggaa
    11161 aaaaccgacg tggcgagaaa gagggtatcg acgactatca ccttctttgc tggcatttgc
    11221 tggatgtggc cgcaatgggc tatttaatgg ttaaaagaaa ttgcttcggg ctggctgatt
    11281 attttcgtca attagggatt tctgacaagg aacaggcagc tcaatttttc gcctggttgc
    11341 tgtgctggca cgatattgga aaatttgccc gctcttttca acaactttac ctgccccctg
    11401 aattcaagat tcaggaaggt gccagaaaaa attacgaaaa aatctctcat tcaacgctgg
    11461 gctactggtt gtggaatcat tatttaagtg aatgtcagga gttacttcct tcatcttcgc
    11521 tctctccgcg caaacttagg cgagtgatag aaatgtggat gcccgtgacg acagggcatc
    11581 atggtcggcc ccctgatcgt atggatgaat tggataattt tctgcctgaa gacaaagctg
    11641 cagcacgaga ttttctcctt gaaatcaagc cactgtttcc gcgaatagag atccccacat
    11701 tctgggatga tgacgagggc gttgaactta taaaacaact ttcctggtat atctctgcaa
    11761 ccgtcgtact tgcagactgg acagggtcgt caacgcgatt ttttccacgc gtcgcacagg
    11821 caatggatat tgaagattac tggcaaaagg ctttagttca ggctcaaaac gcattaaccg
    11881 tctttccgcc acaagcagaa accgcgcctt tcaccggaat taatacgctg tttcctttta
    11941 ttgagaatac gacaccgtta cagcaaaagg tgctggatct ggatatcaat cagccaggac
    12001 cacagttgtt tattctggaa gatgtgaccg gcgcaggtaa aacagaagcg gcgcttatcc
    12061 tggcgcacag gttgatggct gcgggtaacg ctcaggggct gttttttggc ctgccaacaa
    12121 tggcaacggc caatgccatg tacgatcgac tggtcaaaac ctggcttgct ttctattcgc
    12181 cagaggtgtt ccccgctcca gcggagataa accggcgcaa tacggtgcgg ttttcccgga
    12241 agtaaaccgg acgcactgga tgcgatgatg ggtatcactt agaattcccc gcccctgcgg
    12301 tagaactccc agctcccatt ttcaaaccca tcaagacgcc ttcgccagct ccttcaccaa
    12361 cggtagcatt atccgcataa cgtcacggca gcgacgttct attcttccag gaagtgcctt
    12421 atcaatatgt tgttgattat ccagtcttac gtcatgccag ctatttcccg ccgggaaggc
    12481 aggtgttttt gcgcgttgct gataaccatc cttatttcca agattccagt tagtcgcttc
    12541 caccgaaagt acagcaatgc ccgctttgtc gaatatttct gcgtcattac aacacccagt
    12601 gcctttcgga taatttttat tcaaacccgg attggtcgtt gcggctattc cgtgactgcg
    12661 tgcaattgcc agcgccctgt cgcgcgttaa tttccttact gcttcagggg tttttacacc
    12721 gctgttgaaa tacaatttat cgccaacaat taagttatcg agattaatca ccagcagcgt
    12781 atttttcttt tcggtgtcac tcatccgctt gagtaaattc tcagcgccta attttccttc
    12841 ctcttcgccg ctggtcgcca caaatcgaat accatactcg gtaggcgtat ttttcaggcg
    12901 ttctgccagt tccagcatga cacctaaacc tgcggcgtta tcatctattc cttgtaacgt
    12961 cagtccgccg agattggcat ctgcgtcagc atcgctcagc ggggcgtaag tatccagatg
    13021 cgccataatg atgatctgct gcggcgcttt gccttcatga gcggcaatca ccgtacttcc
    13081 tgtcacgtta tgccagttct tgcgattatc gcgggcggta taaatatacc gactattaaa
    13141 tgtccgaata tcactgcgat aacccatttg ctgaaactgt tggcgaatat aatcggcaga
    13201 taacatttct gctggagttc cggtcatgcg tcccggaaag aaagtagcaa tatgtcgtgc
    13261 ctgggtatta gcaaaatcgc caggtttagg tgacgaggcg tgtacgggga gaataaagca
    13321 tacgccgagc gccagggcag cggtacggtg gcgcaatgcg gaaaacatag tgagtcctta
    13381 aataccatgc aaattttttt accgccatag tatgaaactg ccgctgcgct aaaacaattt
    13441 caaatcttcc taaacgcccg aaatccggtg ccttaagcac tttttgatat tagctttgcc
    13501 aaatcgttat tccgttaagg aactactcat tctaattggt aatttcattc gttctcttac
    13561 gctccctata gtcgaaacat ctgatggcaa gaaaatagcg gtattgcaaa ggaacggtta
    13621 tggatcaaaa acgacttact cacctgcggc aactggaggc ggaaagcatc cacattattc
    13681 gcgaggtggc ggcagaattc tcaaatccgg tgatgctcta ctctatcggt aaagattcca
    13741 gcgtcatgct gcatctggcg cgcaaggcgt tttatccagg tacgctgcct ttcccgttgc
    13801 tgcatgtcga taccggctgg aaattccgcg agatgtatga gttccgcgat cgtacggcga
    13861 aagcctacgg ctgcgagctg ctggtgcata aaaacccgga aggcgtggcg atggggatta
    13921 atccattcgt gcacggcagc gcgaaacata ccgatattat gaaaactgaa ggcctgaaac
    13981 aggcgctgaa caaatacggt tttgatgccg ccttcggtgg tgcgcgccgt gacgaagaga
    14041 aatcccgcgc taaagagcga atctactctt tccgtgaccg cttccatcgc tgggatccga
    14101 aaaatcagcg cccggagctg tggcacaact acaacgggca aattaacaaa ggcgaaagca
    14161 tccgcgtctt cccgctctct aactggaccg agcaggatat ctggcaatac atctggctgg
    14221 aaaatatcga cattgtgccg ctgtatctcg ctgcggaacg tccggttctg gaacgcgacg
    14281 gtatgttgat gatgattgat gacaaccgta tcgacctgca accgggcgaa gtgatcaaaa
    14341 aacggatggt gcgtttccgt acgctgggct gctggccgct gaccggtgcg gtggagtcaa
    14401 atgcacaaac actgccggaa atcatcgaag agatgctggt ttccaccacc agtgaacgtc
    14461 agggtcgcgt gattgaccgc gaccaggcgg gatccatgga gctgaaaaaa cgtcaggggt
    14521 atttttaaga tgaacaccgt acttgcacaa caaatcgcca atgaaggcgg cgtcgaagcc
    14581 tggatgattg cgcaacaaca taaaagcctg ctgcgttttc tgacctgtgg tagcgtcgat
    14641 gacggcaaaa gtaccctgat tggtcgtctg ctgcacgata cccgccaaat ctacgaagat
    14701 cagctctcat cgctgcataa cgacagtaag cgtcacggca cccagggcga aaagctggat
    14761 ctggctctgc tggtggacgg cctgcaagct gagcgcgaac agggcatcac tattgatgtg
    14821 gcctaccgct atttctctac cgagaagcgt aaatttatta tcgccgacac cccagggcac
    14881 gagcagtaca ctcgcaatat ggcgactggc gcatcgacat gtgaactggc gatcttactg
    14941 attgatgccc gtaaaggcgt gctcgatcaa acccgtcgtc acagttttat ctccacactg
    15001 ttggggatca aacatctggt cgtggcgatc aacaaaatgg atctggtgga ttacagtgaa
    15061 gagacgttca cccgtattcg tgaagattat ctgacctttg ccgggcagct gccggataat
    15121 ctggatatcc gctttgtgcc gctctccgca ctggaaggtg acaacgtggc ttcgcaaagt
    15181 gaaagtatgc cgtggtacag cggtctgaca ctgctcgaag tgctggaaac cgtggagatc
    15241 cagcgagtgg tggatgccca gccaatgcgc ttcccggtgc agtacgttaa ccgcccaaat
    15301 ctcgattttc gtggctacgc cggaatgctg gcatccggtc gcgtggaagt cgggcaacgt
    15361 gtaaaagtgc tgccctctgg tgtggaatca aacgtcgcgc ggatcgtgac ttttgatggt
    15421 gatcgcgaag aagtctttgc cggagaagcg atcaccctgg tgctgacgga tgagatcgat
    15481 atcagccgtg gtgatctgct gctggcggca gacgaagcgt taccggcggt gcagagtgcg
    15541 tcggtggatg tggtatggat ggcggaacag ccgctttccc cgggccagag ttatgacatc
    15601 aaaattgccg gtaagaagac gcgtgctcgt gttgatggca ttcgttatca ggttgatatt
    15661 aataacctta cccaacgcga agttgaaaac ctgccgctga acggcatcgg cctggtggat
    15721 ctcacttttg acgagccact ggtgttagat cgctatcagc aaaatccggt taccggtggg
    15781 ctgattttta tcgatcgcct gagcaatgtg accgtgggtg ccggtatggt gcacgagcca
    15841 gttagccagg caacagctgc gtcatctgaa ttcagtgcat tcgaactgga attgaatgca
    15901 ctggttcgtc gccactttcc gcactggggc gcgcgcgatt tgctggggga taaataatgg
    15961 cgctgcatga cgaaaacgtc gtctggcata gtcatccggt cactgtgcaa caacgcgaac
    16021 tacaccacgg tcatcgtggt gtagtgctgt ggtttaccgg cctctccggg tccggtaaat
    16081 caacggtcgc cggggcgctg gaggaggcgt tacataaact cagcgtcagt acgtatctgc
    16141 tggatggcga caatgttcgc cacggattat gcagcgatct cggttttagc gatgccgatc
    16201 gtaaagagaa tatccgtcgc gtcggtgaag tggcgaattt gatggttgaa gccggactgg
    16261 tggtgctgac cgcatttatc tcgccacacc gcgccgaacg ccagatggtt cgcgaacgcg
    16321 taggagaagg gcgctttatt gaagtgtttg tcgatacgcc gctggcgatt tgcgaagccc
    16381 gcgatccaaa aggcttatat aagaaagcgc gtgccggtga actgcgcaac tttacgggaa
    16441 tagattccgt ttacgaagcg cctgaatcgg cagaaattca tctcaatggt gaacaattag
    16501 taacaaattt ggtacagcaa ttattagatc tgttgagaca gaacgatatt atcagatcct
    16561 gagacaccac cgggtttcca tgcccggttc gtcaaagtaa caggatcaaa tatgcgtaat
    16621 agccataaca ttacactaac aaataacgac agccttactg aggatgaaga aactacatgg
    16681 tcactgcctg gtgccgtggt cggttttatc tcctggttat ttgcgctggc gatgccgatg
    16741 ttgatttatg gctctaacac gctgttcttc tttctctaca cctggccttt ctttctggca
    16801 ctgatgcctg tcgcggtagt ggtggggatt gcgctgcatt cattgatgga cggaaagcta
    16861 cgctacagta ttgttttcac cctggtgaca gtaggcatta tgtttggcgc actgtttatg
    16921 tggctactgg gctaatttgt actttcttgt ctcctctgtt cataattcaa accgtaacta
    16981 ataatgagat tatgttctgc acgccctggg tatacgtaac aatggacaaa tgtggtacat
    17041 ttgcccgcgt tgtcgcggta tccccaacag aggatgtaga gtcgtcttcg gatgcatggg
    17101 atgatgatgc agtttttcag ggggcaggat gggtaaacta acgctgctgt tgctggctat
    17161 tctggtctgg ctacagtatt cgctgtggtt cggtaagaac ggtatacatg actatacccg
    17221 cgtcaatgat gatgtggcgg cactgcaagc tacaaacgcg aaacttaaag cgcgaaacga
    17281 tcaacttttt gccgaaattg acgatctcaa tggcggccag gaggcgctcg aagagcgtgc
    17341 gcgtaatgaa ctcagcatga ccaggccggg cgaaactttt tatcgtctgg tgcctgacgc
    17401 gtcgaagcgc gcacagtctg cggggcaaaa caatcgataa atcagcccgg gaattaacat
    17461 ggcaaccact catttggatg tttgcgccgt ggttccggcg gccggatttg gccgtcgaat
    17521 gcaaacggaa tgtcctaagc aatatctctc aatcggtaat caaaccattc ttgaacactc
    17581 ggtgcatgcg ctgctggcgc atccccgggt gacacgtgtc gtcattgcca taagtcctgg
    17641 cgatagccgt tttgcacaac ttcctctggc gaatcatccg caaatcaccg ttgtagatgg
    17701 cggtgatgag cgtgccgatt ccgtgctggc aggtctgaaa gccgctggcg atgtgcagtg
    17761 ggtattggtg catgacgccg ctcgtccttg tttgcatcag gatgacctcg cgcgattgtt
    17821 ggcgttgagc gaaaccagcc gcacgggagg gatcctcgcc gcaccagtgc gcgatactat
    17881 gaaacgtgcc gaaccgggca aaaatgccat tgctcatacc gttgatcgca acggcttatg
    17941 gcacgcgctg acgccgcaat ttttccctcg tgagctgtta catgactgtc tgacgcgcgc
    18001 tctaaatgaa ggcgctacca ttaccgacga agcctcggcg ctggaatatt gcggatttca
    18061 tccgcagttg gtcgaaggcc gcgcggataa cattaaagtc acgcgcccgg aagatttggc
    18121 actggccgag ttttacctca cccgaaccat ccatcaggag aatacataat gcgaattgga
    18181 cacggttttg acgtacacgc ctttggcggt gaaggcccaa ttatcattgg tggcgtacgc
    18241 attccttacg aaaaaggatt gctggcgcat tctgatggcg acgtggcgct tcatgcgttg
    18301 accgatgcat tgcttggcgc ggcggcgctg ggggatatcg gcaaactgtt cccggatacc
    18361 gatccggcat ttaaaggtgc cgacagccgc gagctgctac gcgaagcctg gcgtcgtatt
    18421 caggcgaagg gttataccct tggcaacgtc gatgtcacta tcatcgctca ggcaccgaag
    18481 atgttgccgc acattccaca aatgcgcgtg tttattgccg aagatctcgg ctgccatatg
    18541 gatgatgtta acgtgaaagc cactactacg gaaaaacttg gatttaccgg acgtggggaa
    18601 gggattgcct gtgaagcggt ggcgctactc attaaggcaa caaaatgatt gagtttgata
    18661 atctcactta cctccacggt aaaccgcaag gcaccgggct gctgaaagcc aatccggaag
    18721 actttgtggt ggtggaagat ttgggctttg agcctgatgg tgaaggtgag catattctgg
    18781 ttagaatcct caaaaacggc tgcaataccc gttttgtggc ggatgcactg gcgaaattcc
    18841 tgaaaattca ttcccgtgaa gtcagcttcg ctgggcaaaa agacaaacat gctgtaacgg
    18901 aacagtggtt atgtgctcgc gtgccgggca aggaaatgcc cgatctgagc gcctttcaac
    18961 tggaaggctg ccaggtgctg gagtatgcgc ggcacaagcg caagctgcgt ttaggggcgc
    19021 tgaaaggtaa cgcctttacc ctggttctgc gcgaagtgag caatcgcgat gacgttgaac
    19081 aacgtctgat cgatatttgc gtaaaaggtg taccgaacta cttcggtgcc caacgttttg
    19141 ggattggcgg tagcaacttg cagggcgcgc tgcgctgggc gcaaaccaat actccggtgc
    19201 gcgatcgcaa taaacggagt ttttggttgt cggcagcccg cagtgcgttg tttaatcaga
    19261 ttgttgctga gcgcctcaaa aaagcagacg ttaatcaagt tgttgacggc gatgcgctac
    19321 aattagccgg acgtggtagc tggtttgtag caaccaccga agaactggcg gaattacagc
    19381 gtcgcgtcaa cgataaagag ctgatgataa ccgccgcatt gccgggcagt ggcgaatggg
    19441 gaactcagcg tgaagcgctg gcattcgaac aagcagctgt cgccgcagaa actgaattac
    19501 aagctttact ggtgcgcgaa aaagttgaag ccgcgcgcag agcgatgctg ctgtatccgc
    19561 aacaattaag ctggaactgg tgggatgacg tcaccgtaga gatccgtttc tggcttccgg
    19621 cgggtagttt tgcaaccagc gttgtcaggg aacttatcaa cacaacaggt gattatgcgc
    19681 atattgctga gtaatgatga cggggtacat gcacccggta tacaaacgct ggcgaaagcc
    19741 ttgcgtgagt ttgctgacgt tcaggtggtc gcccccgatc gtaaccgcag cggcgcttca
    19801 aattctctga cactggaatc ctccctgcgc acgtttacct ttgaaaatgg tgatattgct
    19861 gtgcaaatgg gaaccccgac cgattgtgtc tatcttggcg tgaatgctct gatgcgtccg
    19921 cgcccggaca ttgttgtgtc cggaattaac gccgggccga atctggggga tgatgttatt
    19981 tattccggta cggtagccgc cgcgatggaa ggccgtcatt taggttttcc ggcgctggcc
    20041 gtctcgcttg acgggcataa acattacgac actgccgcgg cggtaacctg ttcaattttg
    20101 cgcgcactgt gtaaagagcc gctgcgcacc ggacgtattc ttaatattaa cgttcaggat
    20161 ttacccttgg atcaaatcaa aggtattcgc gtgacgcgct gcggtacacg acatccggca
    20221 gatcaggtga tcccgcagca agatccgcgc ggcaatacgc tgtactggat tggcccgccg
    20281 ggcggtaaat gtgatgccgg tccggggacc gattttgctg cggtagatga gggctatgtc
    20341 tccatcacgc cgctgcatgt ggatttaact gcgcatagcg cgcaagatgt ggtttcagac
    20401 tggttaaaca gcgtgggagt tggcacgcaa tggtaagcag acgcgtacaa gcacttctgg
    20461 atcaattacg tgcgcaaggt attcaggatg agcaggtgct gaatgcactt gccgccgtgc
    20521 cgcgtgaaaa attcgttgat gaagcgtttg aacaaaaagc ctgggacaat atcgccttgc
    20581 cgataggtca ggggcagaca atttcgcagc cgtatatggt ggcgcgaatg accgaattac
    20641 tcgagctgac gccgcagtcg cgggtgctgg aaattggcac tggttcggga tatcaaacgg
    20701 caatcctggc gcatcttgtc cagcatgttt gctcggttga acggattaaa ggcttgcagt
    20761 ggcaggcacg tcgccgcctg aaaaatcttg atttacataa tgtttcaacc cgtcatggtg
    20821 atggatggca aggttggcag gcacgtgcgc cgtttgacgc tatcattgtt acggcggcac
    20881 cgccggaaat tccaactgcg ctaatgacgc agctggacga aggcgggatt ctcgtcttac
    20941 ccgtagggga ggagcaccag tatttgaaac gggtgcgtcg tcggggaggc gaatttatta
    21001 tcgataccgt ggaggccgtg cgctttgtcc ctttagtgaa gggtgagctg gcttaaaacg
    21061 tgaggaaata cctggatttt tcctggttat tttgccgtag gtcagcgtat cgtgaacatc
    21121 ttttccagtg ttcagtaggg tgccttgcac ggtaattatg tcactggtta ttaaccaatt
    21181 tttcctgggg gataaatgag cgcgggaagc ccaaaattca ccgttcgccg cattgcggct
    21241 ttgtcactgg tttcgctatg gctggcaggc tgttctgaca cttcaaatcc accggcaccg
    21301 gtcagctccg ttaatggcaa tgcgcctgca aatactaatt ctggtatgtt gattacgccg
    21361 ccgccgaaaa tggggacgac gtctacagcg cagcaaccgc aaattcagcc ggtacagcag
    21421 ccacaaattc aggctactca acaaccgcaa atccagccag tgcagccagt agctcagcag
    21481 ccggtacaga tggaaaacgg acgcatcgtc tataaccgtc agtatgggaa cattccgaaa
    21541 ggcagttata gcggcagtac ctataccgtg aaaaaaggcg acacactttt ctatatcgcc
    21601 tggattactg gcaacgattt ccgtgacctt gctcagcgca acaatattca ggcaccatac
    21661 gcgctgaacg ttggtcagac cttgcaggtg ggtaatgctt ccggtacgcc aatcactggc
    21721 ggaaatgcca ttacccaggc cgacgcagca gagcaaggag ttgtgatcaa gcctgcacaa
    21781 aattccaccg ttgctgttgc gtcgcaaccg acaattacgt attctgagtc ttcgggtgaa
    21841 cagagtgcta acaaaatgtt gccgaacaac aagccaactg cgaccacggt cacagcgcct
    21901 gtaacggtac caacagcaag cacaaccgag ccgactgtca gcagtacatc aaccagtacg
    21961 cctatctcca cctggcgctg gccgactgag ggcaaagtga tcgaaacctt tggcgcttct
    22021 gaggggggca acaaggggat tgatatcgca ggcagcaaag gacaggcaat tatcgcgacc
    22081 gcagatggcc gcgttgttta tgctggtaac gcgctgcgcg gctacggtaa tctgattatc
    22141 atcaaacata atgatgatta cctgagtgcc tacgcccata acgacacaat gctggtccgg
    22201 gaacaacaag aagtgaaggc ggggcaaaaa atagcaacca tgggtagcac cggaaccagt
    22261 tcaacacgct tgcattttga aattcgttac aaggggaaat ccgtaaaccc gctgcgttat
    22321 ttgccgcagc gataaatcgg cggaaccagg cttttgcttg aatgttccgt caagggatca
    22381 cgggtaggag ccaccttatg agtcagaata cgctgaaagt tcatgattta aatgaagatg
    22441 cggaatttga tgagaacgga gttgaggttt ttgacgaaaa ggccttagta gaagaggaac
    22501 ccagtgataa cgatttggcc gaagaggaac tgttatcgca gggagccaca cagcgtgtgt
    22561 tggacgcgac tcagctttac cttggtgaga ttggttattc accactgtta acggccgaag
    22621 aagaagttta ttttgcgcgt cgcgcactgc gtggagatgt cgcctctcgc cgccggatga
    22681 tcgagagtaa cttgcgtctg gtggtaaaaa ttgcccgccg ttatggcaat cgtggtctgg
    22741 cgttgctgga ccttatcgaa gagggcaact tggggctgat ccgcgcggta gagaagtttg
    22801 acccggaacg tggtttccgc ttctcaacat acgcaacctg gtggattcgc cagacgattg
    22861 aacgggcgat tatgaaccaa acccgtacta ttcgtttgcc gattcacatc gtaaaggagc
    22921 tgaacgttta cctgcgaacc gcacgtgagt tgtcccataa gctggaccat gaaccaagtg
    22981 cggaagagat cgcagagcaa ctggataagc cagttgatga cgtcagccgt atgcttcgtc
    23041 ttaacgagcg cattacctcg gtagacaccc cgctgggtgg tgattccgaa aaagcgttgc
    23101 tggacatcct ggccgatgaa aaagagaacg gtccggaaga taccacgcaa gatgacgata
    23161 tgaagcagag catcgtcaaa tggctgttcg agctgaacgc caaacagcgt gaagtactgg
    23221 cacgtcgatt cggtttgctg gggtatgaag cggcaacact ggaagatgta ggtcgtgaaa
    23281 ttggcctcac ccgtgaacgt gttcgccaga ttcaggttga aggcctgcgc cgtttgcgcg
    23341 aaatcctgca aacgcagggg ctgaatatcg aagcgctgtt ccgcaagtaa gtaagcatct
    23401 gtcagaaagg ccagtctcaa gcgaggctgg cctttttctt ttgtttggga agcttgtgtt
    23461 tttgtcgcct ggataagaca cgtcagcgtc ctacccggca atcagcattc tatgtgctca
    23521 tcatcttgcg caccaactgc ataaacagct cctgctcttc tccactcaaa cgccccaaaa
    23581 attcctcatc cacgctgtca ccaataggta tcgccgcagc aagtagcttt tctccttcgg
    23641 cagtcagcca gacaaagcgt cgccgcttgt cagcgacgtc atgttcacgt ctgactaagc
    23701 cacgattttc cattcttgcc aacatttctg ctagcgttgc tttggtgctg actgctgctt
    23761 ctatcagtgc cacctgttca ataccaggtt tatcagcaat ggcgcgcatc accgcgtact
    23821 gtggtttggt caggtcaggt aactcgtgct gccagcgagc cgtgtgctgc tgaaaaagct
    23881 gtcgtaactg atggaacgct ttatttcgta acgccatgta aactccgggc taattctctg
    23941 tcgctatcat agcggttttt tagtgacgaa gaagatgttg ttacttttca atccttgccg
    24001 ctgtggaaat gaaggagtat gttaataata acgttcgcat acgaataatt aagagaataa
    24061 gcaatgaaac tgatcgtcgg gatgacaggg gctaccggtg cgcctcttgg tgtggcatta
    24121 ctgcaagcgc tgcgtgagat gccgaatgtc gagactcatc tggtgatgtc gaagtgggcg
    24181 aaaaccacca ttgaactgga aacgccttac agcgctcgcg atgttgctgc cctcgcagac
    24241 ttcagccata acccggcgga tcaggcggcg atcatctcat ccggttcttt tcgtaccgac
    24301 ggcatgatcg ttattccgtg cagtatgaaa acgctcgccg gtatccgcgc tggttacgcc
    24361 gatggcctgg tagggcgcgc ggcggacgtc gtgctcaaag aaggccgcaa actggtgctg
    24421 gtgccgcgtg aaatgccgct tagcaccatc catctcgaaa atatgctcgc actttcacgc
    24481 atgggcgtgg cgatggtgcc gccgatgcct gccttttata accatcccga aacggtagat
    24541 gacattgtcc accatgtggt agcccgcgtg ctggatcaat ttggcctcga acatccccac
    24601 gccaggcgct ggcaaggatt accgcaggcc cggaattttt ctcaggagaa tgaataatgg
    24661 catttgatga tttacgcagc tttttacagg cgcttgatga ccacggccag ttactgaaaa
    24721 tcagcgaaga agtgaacgct gagccggatc tggcagcagc agctaacgcc accgggcgta
    24781 tcggcgacgg cgcgcccgcg ctgtggtttg ataatattcg cggctttacc gatgcccgcg
    24841 tggcgatgaa taccatcggt tcctggcaga accacgcgat ttcccttggc ctgccaccaa
    24901 acaccccggt caaaaagcag attgatgagt ttatccgccg ctgggataac ttcccgattg
    24961 ccccggagcg ccgcgccaat ccagcctggg cgcagaacac cgtcgatggc gaagagatca
    25021 acctgttcga tattctgccg ctgtttcgcc tgaatgatgg cgatggcggt ttctatctcg
    25081 acaaagcgtg cgtggtttcc cgcgatccgc tcgacccgga taacttcggc aagcagaacg
    25141 tcggcatcta ccgcatggaa gtgaagggca agcgtaagct cggcctgcaa ccggtgccga
    25201 tgcacgatat cgccctgcat ctgcataaag cagaagagcg cggtgaagat ctgccgattg
    25261 cgatcacgct cggtaacgat ccgatcatca cgctgatggg ggccacgccg ctgaaatacg
    25321 attagtctga gtatgaaatg gcaggcgcgc tgcgtgaaag cccgtacccg atcgccaccg
    25381 ctccgctgac cggttttgat gtgccgtggg gttcagaagt gatcctcgaa ggggttatcg
    25441 aaagccgtaa acgcgaaatc gaagggccgt tcggtgagtt taccgggcac tactccggtg
    25501 ggcgcaacat gaccgtggtg cgcatcgata aagtctctta tcgcagcaaa ccgattttcg
    25561 aatcactcta tctcggtatg ccgtggaccg aaatcgacta cctatgacta ggtaatttat
    25621 tcaagataac cgggaagaga ttaaaaaaga cgggaataga ttggaatcaa caagttagga
    25681 actcaaggtt ttcaagataa agcgagtcca aaaatgttaa acggttcgat atcaataaaa
    25741 cattcaagat aactcacagg ttcttacaat agcctttaaa gaccgttcaa aaacgattca
    25801 ggtgtttatt atagcgctga tgggtgcaag cgccaaaacc aatgtaattt tagcacagct
    25861 aggcacttgc actgggttat gcgctggctg ctttctgagg tagatttaag tgatcgccct
    25921 tagcttcgga caacgcgtaa atctctctaa ccttttcgtc tggcaaatta ccaaggctta
    25981 acgctatggc tctacgaact ccaactagag cgtgcaactc ttggatgtgc tcatccagaa
    26041 gaatgagtaa ttgctccgct ccttttctcc caagaacatc agctacttgt tgtttttctt
    26101 gtggtttaag aaatgacaag aacattagaa tttgagcatc aacattatcc gcattctctt
    26161 ttttgggttc tttggaatct tttagttcaa catcaccctc tcccgtcatg agccattcca
    26221 aggtaacatt ttcgctttct gctatctttt ttaactgatg cgggcgtggt atcggtgcgt
    26281 aatcccgcct gttgtaataa ttttttaaag tgccttcatt gatcccccat gcccgagcag
    26341 ccgcatttct tgaagggtat cgttctacta acctaaagat tctttcgata atcggttctt
    26401 ttccatcggc aggaaaagaa ccttggggtg cttttctcat tcttggttcc tttctatcta
    26461 attcattgat aaataataaa attaaaaaat aaaccataaa aacaccaaag aaccctttac
    26521 agggaatctt tggtatctta atctttatcc atacggatat ccgaccggat atccgcgcgg
    26581 gtaattttgt aggttaaagg aaagatgaga caaagagaag atagtgaccg cgattggcct
    26641 tcgctgaaga ttcagctggc gctcaaagat aacgggtggt cttccttccg ggagctttca
    26701 ctccatgctg gttatgctca ggattcactg aaagcggtag ttcgcacacc aaacaaaaaa
    26761 tacgaacgcg taatttctga cgctataggt gtaccgccag aaatcatctg gcccagccgg
    26821 tatcgtaacg atggatttat caggaaggcg tcgtgatatg cagtcgtacg ttaccgtaaa
    26881 tgacctgctc ggcgtaccag gaatgccagc caccacaaaa ggcatccgac aggcgttaca
    26941 gcgtttttct cgtgatcttg gtgacgttag tcgccgtcgg gaaggcacaa aagccattga
    27001 gtaccacatt gattgtctgc ctgaaataac acgaaaggca ctgcgtgaac gctatgtgga
    27061 acagctggtg gctacagaga acaatgtttc tgaagtgaaa gctgttacca gaaaaacacg
    27121 caatcctgac gctgtccagg caatcgaagc atatcgcggt tctccacaac tgatggaaga
    27181 acgcctgaat gcgctgaccg aaaaccagcg ctgggtatct gaagcaagag ctgcgctggt
    27241 ggtggaagtg ctgaagctgg aaagcgccgg taaccccggg cgactgaaag ccattaactt
    27301 tcttgttgaa aaagcccgta aaggtgagct gccggagcgc ctgcaacagg ccgcagttaa
    27361 cgccaatgca aaacgtggcg ctaatcgtac tatcagccgc gatccacttt accagtgggt
    27421 tctgaaatac aaccagtcgc agaacgccgc cgaacggctg ctgttactgg caccgggaaa
    27481 acgcgacgaa ataaaaccgg aagaaatcag ctggctgcct gaatttctgg cgcagtatcg
    27541 ccaggtgaac ggcagaccca tgtcagaagc ctacgaggat tttgtcgcag aatggcagcg
    27601 tcgccatgcg gacgaaccgt acatgctgga ggtgatgcct tcctatgatg ttgtccggta
    27661 tgccatgaaa aaactgccgg aagtggtgaa acaaaaaggg cgcgttaccg gcagtgaata
    27721 ccgtcagctt gaaggattca cccgccgcga ctggacagct atgccggtga attatgtctg
    27781 gattggtgac ggtcacggta tgaagctgaa atgtgcgcat ccgatccacg ggcgtccatt
    27841 ctcaccggaa gtcacgtttg tgatcgatgg tggaacacgg tttgttgttg gctggagtct
    27901 tgaccttgcc gaaaacgtgt tcgccgttgc cggtgcgata cagcacggca ttcgcaacca
    27961 cggaaaaccg ttcctgtatt actcggataa cggttccggt gaaacggctg acatgctgga
    28021 caaagaggtc gtgggtattc tgccacgcct ggggattaaa caccctaccg gtattgccgg
    28081 taatccacag ggacgcggca ttattgaacg cctgaaccgg acactgccga tgcgtatagc
    28141 caggaggtat cgcacctatt ttggcaaggg tgctgaccgg gaatcgttgc gtgtacttaa
    28201 ccgtgatctg cgttcagcgt ttaacgccct gcaacaggac aagcctctga acgaccgcca
    28261 gaaagcggca atgcgcgagc tgccatcgtg ggcggagctt attgaggcta ttcgtgaagg
    28321 cgtggaatgg tacaacaacc gcccgcattc tgagctgccg atgaaaccga acggacggca
    28381 ttacagcccg acagagttca gaaaaaaacg ccaggcagaa gaagacaccg aaattgagtg
    28441 gctgagtgat cttgagctgc gcgacatgtt ccgcccaatg gtggaacgac cggtaagacg
    28501 ctgcgaaatt cagtggctga acaatattta ctacgcgccg gagttgcgtg acgaacacgg
    28561 tcgcaaagtg cttatcagtt atgacattca tgacgccgag cgaattacgg tacgtcgcaa
    28621 ggacggcagc tttatctgtg aggcgatatg gaacggcaat aaacgcgcag cctttgctgt
    28681 cagtgctgaa tatcacaaac aacagcagcg tattaaagga atgcgcaaac gcgctgagga
    28741 aaaaattcgt gatgctgaag atgaaggtat tcagattctg gaacacaaac aggctgaacc
    28801 ctggctgagt aatgtttatc gcccggttgg taat
//
LOCUS       DAJVPP010000049        28749 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVPP010000049 DAJVPP010000000
VERSION     DAJVPP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.049"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            353..1711
                     /locus_tag="OQM34_002110"
     CDS             353..1711
                     /locus_tag="OQM34_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCS1452555.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVTNDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1950..3335)
                     /locus_tag="OQM34_002111"
     CDS             complement(1950..3335)
                     /locus_tag="OQM34_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1452556.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OQM34_002112"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OQM34_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1452557.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..4109)
                     /locus_tag="OQM34_002113"
     CDS             complement(3729..4109)
                     /locus_tag="OQM34_002113"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCS1452558.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4464..4898)
                     /locus_tag="OQM34_002114"
     CDS             complement(4464..4898)
                     /locus_tag="OQM34_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCS1452559.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4886..5287)
                     /locus_tag="OQM34_002115"
     CDS             complement(4886..5287)
                     /locus_tag="OQM34_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452560.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5547..6116)
                     /locus_tag="OQM34_002116"
     CDS             complement(5547..6116)
                     /locus_tag="OQM34_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCS1452561.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6861..7004)
                     /locus_tag="OQM34_002117"
                     /pseudo
     CDS             complement(6861..7004)
                     /locus_tag="OQM34_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(7338..7604)
                     /locus_tag="OQM34_002118"
     CDS             complement(7338..7604)
                     /locus_tag="OQM34_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452562.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7745..7951
                     /locus_tag="OQM34_002119"
     CDS             7745..7951
                     /locus_tag="OQM34_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1452563.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8046..8648
                     /locus_tag="OQM34_002120"
     CDS             8046..8648
                     /locus_tag="OQM34_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452564.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            8819..9016
                     /locus_tag="OQM34_002121"
     CDS             8819..9016
                     /locus_tag="OQM34_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452565.1"
                     /translation="MFFTARYTPGSQQTECAGAVIHQKWHAAVHTHPGDNMYVATAIP
                     ALWKRLTQPAGYSFRHPGMSA"
     gene            9848..10765
                     /locus_tag="OQM34_002122"
     CDS             9848..10765
                     /locus_tag="OQM34_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452566.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            10850..11722
                     /locus_tag="OQM34_002123"
     CDS             10850..11722
                     /locus_tag="OQM34_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCS1452567.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            12094..14940
                     /gene="ag43"
                     /locus_tag="OQM34_002124"
     CDS             12094..14940
                     /gene="ag43"
                     /locus_tag="OQM34_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCS1452568.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            15011..15169
                     /locus_tag="OQM34_002125"
     CDS             15011..15169
                     /locus_tag="OQM34_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCS1452569.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            15324..16142
                     /locus_tag="OQM34_002126"
     CDS             15324..16142
                     /locus_tag="OQM34_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCS1452570.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            16484..16957
                     /locus_tag="OQM34_002127"
     CDS             16484..16957
                     /locus_tag="OQM34_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCS1452571.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            16973..17449
                     /locus_tag="OQM34_002128"
     CDS             16973..17449
                     /locus_tag="OQM34_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCS1452572.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            17512..17733
                     /locus_tag="OQM34_002129"
     CDS             17512..17733
                     /locus_tag="OQM34_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCS1452573.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            17896..18270
                     /locus_tag="OQM34_002130"
     CDS             17896..18270
                     /locus_tag="OQM34_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCS1452574.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            18317..18691
                     /locus_tag="OQM34_002131"
     CDS             18317..18691
                     /locus_tag="OQM34_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCS1452575.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            18688..19179
                     /locus_tag="OQM34_002132"
     CDS             18688..19179
                     /locus_tag="OQM34_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452576.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            19191..19388
                     /locus_tag="OQM34_002133"
     CDS             19191..19388
                     /locus_tag="OQM34_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452577.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            19473..20033
                     /locus_tag="OQM34_002134"
                     /pseudo
     CDS             19473..20033
                     /locus_tag="OQM34_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            20105..21109
                     /locus_tag="OQM34_002135"
     CDS             20105..21109
                     /locus_tag="OQM34_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCS1452578.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            21391..21672
                     /locus_tag="OQM34_002136"
                     /pseudo
     CDS             21391..21672
                     /locus_tag="OQM34_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(21822..21909)
                     /locus_tag="OQM34_002137"
     tRNA            complement(21822..21909)
                     /locus_tag="OQM34_002137"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21873..21875),aa:Ser,seq:gga)
     gene            22144..23082
                     /gene="ghrA"
                     /locus_tag="OQM34_002138"
                     /pseudo
     CDS             22144..23082
                     /gene="ghrA"
                     /locus_tag="OQM34_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            23137..23874
                     /gene="ycdX"
                     /locus_tag="OQM34_002139"
     CDS             23137..23874
                     /gene="ycdX"
                     /locus_tag="OQM34_002139"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1452579.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            23898..24452
                     /gene="ycdY"
                     /locus_tag="OQM34_002140"
     CDS             23898..24452
                     /gene="ycdY"
                     /locus_tag="OQM34_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1452580.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            24554..25045
                     /locus_tag="OQM34_002141"
     CDS             24554..25045
                     /locus_tag="OQM34_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1452581.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(25178..25884)
                     /locus_tag="OQM34_002142"
                     /pseudo
     CDS             complement(25178..25884)
                     /locus_tag="OQM34_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(25911..26327)
                     /gene="csgF"
                     /locus_tag="OQM34_002143"
     CDS             complement(25911..26327)
                     /gene="csgF"
                     /locus_tag="OQM34_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCS1452582.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(26352..26741)
                     /gene="csgE"
                     /locus_tag="OQM34_002144"
     CDS             complement(26352..26741)
                     /gene="csgE"
                     /locus_tag="OQM34_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCS1452583.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(26746..27396)
                     /gene="csgD"
                     /locus_tag="OQM34_002145"
     CDS             complement(26746..27396)
                     /gene="csgD"
                     /locus_tag="OQM34_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1452584.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQYLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            28148..28603
                     /gene="csgB"
                     /locus_tag="OQM34_002146"
     CDS             28148..28603
                     /gene="csgB"
                     /locus_tag="OQM34_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCS1452585.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            28644..>28749
                     /locus_tag="OQM34_002147"
     CDS             28644..>28749
                     /locus_tag="OQM34_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCS1452586.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT         7125 a         7257 c         7332 g         7035 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtgacg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttcg gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcttttt
     2461 ctgtccgggc tgtaagcgaa ccacactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc aatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagc
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttggg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtcg ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc
     3661 gttgaagcta tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggagg cagcaacgag cagctgttta aattcgggag gataattagg gcagcctttt
     4081 cgcctgccgg gagtcacatt tttctgcata tctgacactt tggttcccac tacttatttg
     4141 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtacgctta
     4201 ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg gaaacgcaca
     4261 cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac gaatggaatg
     4321 acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa aggccaccaa
     4381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa aggcatcacc
     4441 gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc cctggcatct
     4501 tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc gcagcgtggg
     4561 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     4621 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     4681 gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg gtcatgttca
     4741 aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
     4801 cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct
     4861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
     4921 gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa taatcgtggt
     4981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
     5041 gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa gggcactgtt
     5101 gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca gtttcgtatt
     5161 gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca taaggtgcgg
     5221 cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata aaggattttg
     5281 cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
     5341 ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa
     5401 agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact gacgtaaaaa
     5461 acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata cttttggttt
     5521 gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg
     5581 gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag cacttgctga
     5641 aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa atgcaccagc
     5701 gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt aatcatcccg
     5761 gccagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg gttgaccagc
     5821 agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc ccagacgtaa
     5881 tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg
     5941 attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt tgctgccggt
     6001 acatccggga gacgcaggtc aacccgcaat gccgtcaggc ggggatgaac attcagtgcg
     6061 tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta ggtcatggtt
     6121 aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa
     6181 gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg tcgtctccgg
     6241 actgacggtt gaattttcag cgtcgttttt taatgtaaaa aaccggttac gggtaaggct
     6301 gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga aacgcgatta
     6361 ctcataatac agtgtgttgc ccgtattgtg gttctgtcga ccccttcggc tactaccgga
     6421 atacagaccg tatcgttacc atactgctgg cgctgattat tgtggtcctg ctgactactg
     6481 tcagcgtcag tgtgtgtata ctgtgtagct ggtgagataa cggttcatgt atcccctgag
     6541 ctgacagtgt accgtgtata ttaagcgtaa ccgacaggtt ctccttcata ccatattaaa
     6601 ttttccaggg attgccgctg ccttatttcg tcccatacag gaaaactcat ctgggtttct
     6661 gacaaatacg cgaaactcac agcccctcaa tatcaaccac acgttgtaag ccggtaatac
     6721 ctgaaaatca gcctattacc gcccacgcct ccggcaccat tcccttttga tttcgaattc
     6781 tttgttgtcg ctataccgct taatgttccc ggtcctccgg tgcagataac accggagtca
     6841 tcgtagcgct gcttcaatat tcaggtgacg tatttccaga cggaagccgg cgcgcgatcg
     6901 taaagtttgt tcattgtcag ttctgccagc cggttgtcaa cggcagagtt aaaggcttcc
     6961 agctccgcaa gggtaagttt ataacgcgtg tgggaattca cttttcccgc gtctcctgac
     7021 gtattacggg ctgccactta cctggtgtcc cgcaatacag ccttagtgtt ccgttatcgg
     7081 ggatccggac gcaccgcaac aaccggggaa gtatttctgg tttagagcag tgtcaagagg
     7141 gatttctcag cccctcactc accggctcag tctcagggca ggagcattaa tacgctcagt
     7201 tcatgtctta tacaggaatg ggcacagggg gagctggcgt gtcactgctg attttacgca
     7261 tcagcgttca tgtatctgca ctctgataca tgaacgattc acagcgctct gttaccggag
     7321 agatttactc tcttagctta ctctggcaaa tcctttacgt tactctctga tgacttctta
     7381 tatttataat accaatcaga cagccttatc ccatcggtga tatcagtctc ctcctcgcca
     7441 ggaacgggct gactcactgt aactacctcc gaaaacaact ctgcaattat cagaacgcct
     7501 gcttctctcc ctgtcctcac gaaaactatc ccctctttat cgcgcgtgcg tgcggaagca
     7561 tcttttcgca acaaccaccc ggtataccgg tacggctcag ccaccgcaac ctccccgttt
     7621 atctcctggc ggtcacattc ccgattattt tttacgtttc tccccggttg ttatgccggt
     7681 gaaggtggcg cgctgttttc atgaccacac cggctggttt aacaacatcc ggaggaacat
     7741 tctcatggct accccagttt cactgatgga tgaccagatg gtcgacatgg catttatcac
     7801 tcaactgacc ggcctgacag ataagtggtt ttacaggctc atcagggatg gggcctttcc
     7861 ggctcccatc aagctgggcc gcagctcccg ctggctgaaa agtgaagtgg aagcctggct
     7921 acaggcgcgt attgcacagc cccgcccgta atttctgacc gttatccgtt cacccgcagc
     7981 agacgtaatc cccggcctgc cggcggcatt ctgctgcctg ttatatcccc gtgaggaata
     8041 tgaaaatgaa acaacagtat cagacccgct acgaatggct ccacgaaagc taccagaaat
     8101 ggctgaccgg cttcacccgg cacgccgtat cctggggcgt gtgtcatccg aatatctact
     8161 acttccataa tctgacgccc ggttgggtgt cattcaatgg tgaaaaaccg gagattgcca
     8221 tcgtacccca gagcctgcac cggctgattt atggccctga caaacgggcc acgccgcccc
     8281 tggatgatga tctgatagtg aatttatgca ccagtgaaca tctgctggtt catcatccga
     8341 tgctggaagg cattctcctg tctgaatgtg aacgtctgag gcagcgttct ctggcaaata
     8401 aactgatcag cctcttccgt cagtttggtg gcacggagtt gcgcctcaaa ctggtctggc
     8461 tttgctggct tgatttaatg accggaaaca gccttgaaga ctggaaagag aacctgaaac
     8521 ggaaatcaga aaaagagctg gaggaatgga tcatcaaccg tcagaggcag agcgcagcac
     8581 tgacggatct gatggatcag tacgtgctcc tggcataccg cacaacggtt gacgatagcc
     8641 gcaactgatg ccggttgctt ccggaaggtg tgacaaaata cggaaatgat gatgctcccg
     8701 gcccccatgc tcacctgcag agcgtgttcg tcgtcatcat actgagccat gacgggcgtc
     8761 ttaagggcat cctgtcattg ctgagcaggc aacaccacca gaggagccac ggacagggat
     8821 gttctttaca gcccgataca cgccaggttc tcagcagact gaatgtgcag gtgcggttat
     8881 tcaccagaaa tggcatgctg cggtacatac ccatcctggt gacaatatgt acgtggcgac
     8941 agcgatcccg gcactgtgga agagactcac tcagcctgca ggatattctt tccggcatcc
     9001 gggaatgtcc gcctgaatgc aaacaggatg atatttgaat ccgtaatact gttctttcat
     9061 cataaataat ttatgcagat acagttaacc gttacagtag ccggtttgtt ttaccgggaa
     9121 ataatatccg tcagactgat accggaacat atctgaaaca cgttatgatg ttttgttatc
     9181 agaaatgttg tatattctta tccgtggcga cagtttaatg ccctctggta tcacctgata
     9241 atgtatatcc tgcgatgatt cgttatattt acgggacacg cctcttttca ttgtgctcct
     9301 tttcagactt cattttccgt aagtgttccc cggcataaag gaggtacctt tctggcgttt
     9361 ataactctct ttatcggtga cgccggtaat acagggaata ccacaccgac aaacgaggta
     9421 ttaacagacg atatttcagt gtccataatt tttctgtccg gttaaataac gtcagtctgc
     9481 tgccctctga ctgtaatcat caaagccgga atgcataccg ggttacaacg taaaaaaagt
     9541 gatgctggcc cccggtgcag ctggcgggcg aaacatgttt acctttcaat accccctgtc
     9601 tgcagtctcc actcttccat cctcttaagc tacgtcggcc acatgagtgc acttccatga
     9661 taacaggtat tcctgtcagc ataacgaagc cggttacctt cccttcctcc tgcatttcct
     9721 gtcagaatga ctatacagcg cgcagattgc ctttttattg accatcttaa tataccgtta
     9781 taaacttctt tttatcatat tattatcacc ttattatgtt taatttttaa gcagtcggag
     9841 aaagaggatg gttcagaaaa ttttgtcgga taaggttatg aatgaaagaa caaatgcgta
     9901 ttactcttat tatcttggag agcgtaatat ctccgtactg ccccttaatg tttatgatcc
     9961 cccggaacgt tttatcgctt acataaaaaa aaacagagaa aatctgaaca tcacactttc
    10021 agattttgaa cttgaacaaa taatttctgg tatgcgtctg aaggccctgg ccttcctggt
    10081 ccccctggaa aaaatatcct ggatcgcggg cagtgaacgc gcatgcttat tttcctggta
    10141 tctgttgatg cagtttatcc agaataacag ggcaaaaata agcgcagact tgcttcaaaa
    10201 gaataaactg tatcttaaag aagagtatct ggaagggaat gccttcccgt cggactcatc
    10261 gacacagttc agacaaatac tcagagttct cgatattctt tctgataaga atttgcggga
    10321 tgagtggata atccagacca aagacagatg gatgcgggca tttaaatcaa agagtccttt
    10381 cagttacctc ctcccggaga acgaacatga atgcatatgg acatggaatt atttgaaggg
    10441 aaaaaatatt gcactggaaa aactggccag ttttcctggt tctgctgata tttaccacgc
    10501 aattcacctt tcatttgaca tatgggttac ctgtccactc acctcacctg acgatataaa
    10561 aaatttcaga aacagtttca ataaggccaa ggcgcagcgc aagtacaaaa aaatgcagga
    10621 agataaagtc aatgtacagt tttttcttga cgcagaaacc agggcgcagc tcaaagaatt
    10681 atcgagggtc agaagactca gtaccggcga aatgctgcat gacctgattg ttgaagagta
    10741 taaaaggtac cgacattcca gataatttca gacatatatt atctccgtga gtcccccgcc
    10801 acctttccgg tgtgcggggg ttttgtcttt tttcaccggg aatacatgta tgaatccgtc
    10861 tgatgccatt gaggcaattg aaaaaccgct ctcctccctg ccttactcac tttcccgtca
    10921 catcctggaa catctgcgca aactcaccag tcacgaaccc gtgattggca ttatgggtaa
    10981 aagcggggcc ggtaaatcct cactctgtaa tgcactgttt cagggggagg tcaccccggt
    11041 cagtgatgtt cacgccggca cccgggaagt gcagcgtttc cgtctgagtg gccatggtca
    11101 cagcatggtt atcactgacc tgcccggggt gggcgagagc cgggacagag atgcagagta
    11161 tgaagccctg taccgtgaca ttctgcctga acttgacctg gtactgtggc tgattaaagc
    11221 cgatgaccgt gccctgtctg tggatgagta tttctggcga cacatcctgc accggggaca
    11281 tcagcaggtg ctgtttgtgg tgacgcaggc cgacaaaacg gagccctgcc atgaatggga
    11341 tatggccggt attcagcctt ctcccgcaca ggcacagaat attcgcgaaa aaacggaggc
    11401 ggtattccgt ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg
    11461 ggaactggat acgctggtca gtgcgctaat gacagcactt cccgaccatg ccgccagtcc
    11521 cctgatgacc cgcctgcagg acgagctgcg cacggaatct gtccggggtc aggcccgtga
    11581 acagtttacc ggtgcggtgg accggatatt tgacacggcg gagagcgtct gtgttgcctc
    11641 tgttgcccgt acggttctgc gtgccgtccg tgacacggtg gtctctgttg cccgcgctgt
    11701 atggaactgg attttcttct gaacagtctg ccagggaaat gcctgataac attacgttaa
    11761 ccaactgaaa acatgacaca aacccctcat actcctcctg tttctcttgc gtgaccgcct
    11821 tgtcgttaat agaataaaac tatcgataaa acagatatcg atcgttgcta tcgatcgttt
    11881 atagtggtta atggtctgcg aaaaggcagc tgaatctctt catcatgcag aacggaattg
    11941 cacacaacgg actgatactt ctctggcgga tgagagaggg gaagcattta tgtccgggaa
    12001 aaaccacttt atctgaccat gctgttttgt acctgccggt atccactttt gtgggtaccg
    12061 gcttttttat tcaccctctg taaggaaaag ctgatgaaac gacatctgaa caccagctac
    12121 aggctggtat ggaatcacat tacgggcacc ctggtggtgg cttccgaact ggcccgctca
    12181 cggggaaaac gcgccggtgt ggcggttgtg ctgtctcttg ctgctgtcac atcagtcccg
    12241 gcactggctg ctgacacgat tgtacaggcg ggagaaaccg tgaacggcgg aacactgaca
    12301 aatcatgaca accagattgt tctcggtacg gccaacggaa tgaccatcag tagcggtctg
    12361 gagtatgggc cggataacga ggccaatacc ggcgggcaat ggatacaaaa tggcggtatc
    12421 gccaacaaca ctactgtcac cggtggtggt cttcagagag tgaatgccgg aggaagcgtt
    12481 tcagacacgg ttatcagtgc cggaggcgga cagagccttc aggggcaggc agtgaatacc
    12541 actctgaacg gcggtgagca gtgggtacat gaaggcggga ttgcaacggg taccgtcatt
    12601 aatgagaagg gctggcagac cgtcaaatcc ggcgcaatgg caaccgacac ggttgtgaat
    12661 accggcgcgg aagggggacc agatgcagaa aatggtgata ccgggcagtt tgttcgcgga
    12721 aatgccgtac gtaccactat caatgaaaat ggtcgtcaga ttgtggctgc tgaaggaaca
    12781 gcaaatacca ctgtggttta tgccggcggc gaccagacgg tacacgggca tgcgctggat
    12841 accacactga atggcggtta ccagtatgtg cacaacggcg gtacggcgtc tggcactgtt
    12901 gtaaacagtg acggctggca gattatcaag gaaggtggtc tggcggattt caccaccgtt
    12961 aaccagaaag gcaaactgca ggtgaacgcc ggtggtaccg ccacacatgt caccctgaaa
    13021 cagggtggag cactggttac cagtactgcg gcaacggttc ttggcagcaa ccgtctgggc
    13081 aatttcacgg tggaaaacgg taaggctgac ggagttgttc tggagtccgg cggtcgcctg
    13141 gatgtactgg aaggccattc agcgcagaaa acacgggtgg atgacggcgg aaccctggca
    13201 gtgtctgccg gtggtaaggc aacaggtgtc accatgacat ccggtggtgc cctgattgca
    13261 gacagtggtg ccactgttga ggggaccaat gccagcggta agttcagtat tgatggcaca
    13321 tccggtcagg ccagcggact gctgctggaa aatggcggca gctttacggt taatgccgga
    13381 ggactggcca gcaacaccac tgtcggacat cgtggaacac tgacgctggc tgccggggga
    13441 agtctgagtg gcagaacaca gctcagtaaa ggtgccagca tggtactgaa tggcgatgtg
    13501 gtcagtaccg gcgatattgt taacgcaggg gagattcgct ttgataatca gacgacaccg
    13561 gatgccgcgc tgagccgtgc tgttgcaaaa ggcgactccc cggtaacgtt ccataaactg
    13621 accaccagta acctcaccgg tcagggtggc accatcaata tgcgtgttcg ccttgatggc
    13681 agcaatacct ctgaccagct ggtgattaat ggtggtcagg caaccggcaa aacctggctt
    13741 gcgtttacaa atgtcggaaa cagtaacctc ggggtggcaa cctccggaca gggtatccgg
    13801 gttgtggatg cacagaatgg cgccaccaca gaagaaggtg cgtttgccct gagtcgcccg
    13861 cttcaggccg gcgcctttaa ctacaccctg aaccgtgaca gcgatgaaga ctggtacctg
    13921 cgcagtgaaa atgcttatcg tgctgaagtc cccctgtata catccatgct gacacaggca
    13981 atggactatg accggattct ggcaggctcc cgcagccatc agaccggtgt aaacggtgaa
    14041 aataacagcg tccgtctcag cattcagggc ggtcatctcg gtcacgataa caacggcggt
    14101 attgcccgtg gagccacgcc ggaaagcagc ggcagctatg gcttcgtccg tctggagggt
    14161 gacctgctga gaacagaggt tgccggtatg tctctgacga caggggtgta tggtgccgca
    14221 ggccattctt ccgttgatgt taaggatgat gacggttccc gcgccggcac ggtccgggat
    14281 gatgccggca gcctgggcgg atacctgaat ctgacacaca cgtcctccgg cctgtgggct
    14341 gacattgtgg cacagggaac ccgccacagc atgaaagcgt catcggacaa taacgacttc
    14401 cgtgcccggg gctggggctg gctgggctca ctggaaaccg gtctgccctt cagtatcact
    14461 gacaacctga tgctggagcc acaactgcac tatacctggc agggacttac cctggatgac
    14521 ggccaggata acgccggtta tgtgaagttc gggcatggca gtgcacaaca tgtgcgtgcc
    14581 ggtttccgtc tgggcagcca caacgatatg agctttggtg aaggcacctc atcccgtgac
    14641 actctgcgcg acagtacaaa acaccgtgtg agtgaactgc cggtgaactg gtgggtacag
    14701 ccttctgtta tccgcacttt cagctcccgg ggtgacatga gcatggggac agccgccgcc
    14761 ggcagtaaca tgacgttctc accgtcccgg aatggcacgt cactggacct gcaggccgga
    14821 ctggaagccc gtgtccggga aaatatcacc ctgggcgttc aggccggtta tgcccacagc
    14881 gtcagcggca gcagcgctga aggttataac ggtcaggcca cgctgaatgt gactttttga
    14941 taatcaggtc actctccttc aggccccggt catcatgacc ggggcttttt actttcaggg
    15001 agcaacagat atgccgggtt gtacatcacg tttattgccc gaaggccctt tctcacgcga
    15061 acaggctgtt gccgtcaaaa cagcttaccg caatgtgttt actgaagatg accagggaac
    15121 atattcccgg ctggttatcc gtaatgctga aggtcagttg cgctggtgat gccggaacac
    15181 agcttaatac tcagctcgcc agttaagggc ttctcagaca ataaacgtca ttcatttcat
    15241 ctgtcaggcc gcgtcttctc cgggagacgc agctttttca tttattacac cagtaaatcg
    15301 taaccaccga taaggagcaa attatgcgac tagccagtcg ttttggttat gcgaaccaga
    15361 tacgccgtga ccgtccgctg acacacgaag aactgatgca ctatgttccc agtattttcg
    15421 gtgaagaccg gcatacttcc cgcagtaaac gatatgcgta cattcccacc atcaccgtac
    15481 tggaaagcct gcagcaggaa ggttttcagc cattcttcgc ctgccagacc cgcgtgcgcg
    15541 acccgggccg ccggggatac acaaaacaca tgctgcgtct gcggcgggcc ggagagataa
    15601 acggagaaca tgtccctgaa attattctgc tcaactctca tgacggtacc tccagctacc
    15661 agatgctgcc gggttacttc agattcgtct gccagaatgg ctgcgtctgc ggtcagtctc
    15721 tgggggaagt gcgtgttcca caccggggaa atgtggtgga gaaagttatc gaaggggctt
    15781 acgaagtggt gggggttttt gaccggattg aggaaaagcg tgatgccatg cagtcgctgg
    15841 tcctgccgcc accggcacgc caggcgctgg cacaggcggc actgacttac cgttatggtg
    15901 acgaacatca gccagtcacc accgctgaca ttctgacacc acgacgcagg gaggattacg
    15961 gtaaagacct gtggagcgca tatcagacca tccaggagaa tatgctgaaa ggcgggattt
    16021 ccggccgcag tgcaaaagga aaacgtatcc acacccgtgc cattcacaac atcgacaccg
    16081 atattaagct caaccgcgca ttgtgggtaa tggcagaaac gctgctggag agcctgcgct
    16141 gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg ccccggaggc
    16201 gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta cccgtctgct
    16261 ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg catctggcca
    16321 tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc gaatatcatt
    16381 catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc cagctcccga
    16441 cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaaa cattgtctca
    16501 gaacaccacc gcttcggcct gtgcaccgga gactggcctg caacaactgg ttgccactcc
    16561 cgtccctgat gaacagcgca tcagcttctg gccgcagcat tttggcctca ttccacagtg
    16621 ggtcaccctg gaaccccgtg tcttcggctg gatggaccgt ctgtgtgaag actactgcgg
    16681 tggtatctgg aatctgtaca ccctgaacaa cggcggggca tttatggcac ccgaaccgga
    16741 tgacgatgat gacgaaacat gggtactgtt caatgccatg aacggtaacc gcgctgaaat
    16801 gagcccggaa gccgccggca ttgctgcctg tctgatgacg tacagtcatc atgcctgtcg
    16861 tacggagtgt tatgccatga cggtccatta ttaccggctg cgggattacg ccctgcagca
    16921 tccggaatgc agcgccatta tgcgcatcat cgactgaatg gaggcaggga caatgcagca
    16981 gctttccttt ctgcccggaa acatgacgcc cggcgagcgc agtctcattc tgcgggccct
    17041 gcaaaccctg gaccgccatc ttcatgaacc cggcgtggcc ttcacctcca cccgtgcggc
    17101 acgggaatgg ctgattctga acatggcggg actggagcgg gaagagttcc gggtgctgta
    17161 tctgaacaac cagaatcagc tgattgccgg tgaaaccctc ttcaccggca ccatcaaccg
    17221 tacggaagtt catccccggg aagtgattaa acgcgccctg taccacaatg ccgctgccgt
    17281 ggtgctggcg cacaatcacc cgtccggtga agtcacaccc agtaaggcag accggcttat
    17341 caccgaacgt ctggtacagg cactgggcct ggtggatatc cgggtgccgg accatctgat
    17401 agtcggtggc aaccaggttt tctcctttgc cgaacatggt ctgctttaac ccgtcacaac
    17461 cacatcacac ctgttttcac ttttatcttc tgtcttcaga ggtatcacat catgagaatc
    17521 atcacccgtg gtgaagccat gcgtattcac caacagcatc ctgcatcccg tctttttccg
    17581 ttctgtaccg gtaagtatcg ctggcacggc agtgctgaag cgtatactgg tcgtgaagtg
    17641 caggatattc ccggcgttct tgccgtgttt gcagaacgcc gtaaggacag ttttggcccg
    17701 tatgtccggc tgatgagtgt caccctgaac tgatgttccg tcacccggga aacgagtacg
    17761 accagatgac ctcagacgcc gctgtggatg aggccattct catcaatgca tacatcttca
    17821 ccgaaaccgg tcagcgcgcc ggctgacctg tcatcgttaa accattctta accacttttc
    17881 agagaggatt ttatcgtgtc agacaaactc tccgggataa ctcatcccga tgacaatcac
    17941 gaccgcccct ggtgggggct tccctgcaca gtgaggccct gttttggcgc tcgtctggtg
    18001 caagagggta accgcctgta ttaccttgcc gaccgcgccg gtatcagagg ccgcttcagc
    18061 gacgcagatg cataccatct ggaccaggcc tttccgctgc tgatgaaaca actggaactc
    18121 atgctcacca gcggtgaact gaatccccga tatcagcata ccgtcacgct gtatgcaaaa
    18181 gggctgacct gcgaagccga caccctcggc tcctgtggtt acgtttatct ggctgtttat
    18241 ccgacaccag cacccgcaac cacctcataa ccccgaagct ctcaccttta gcactaccag
    18301 ttacagagaa cttaagatga aaacattatc cgacacacat gtacgggagg tatctcgctg
    18361 cccgtctccc gtcaccatct ggcagacact gctcatccga ctgctggacc agcattatgg
    18421 cctcacactg aatgacacac cgtttgtcga tgaacgtgtg attgagcagc atattgaggc
    18481 aggcatttca ctgtgtgatg cagttaattt tctcgtggaa aaatacgcgc tggtgcgtac
    18541 cgaccagccg ggattcagca cctgtccccg ctctcagtta ataaacagta ttgatatcct
    18601 ccgggctcgc cgggcgaccg gcctgatgac ccgcgacaat tacagaacgg taaataacat
    18661 taccctgggt aagtatccgg aggcgaaatg atgaaactgg ccctgacact ggaagccgac
    18721 agcgttaacg tacaggcact gaacataggg cgcattgtcg ttgacgtcga tggtattgag
    18781 ctcgctgaac tgattaacat ggtctgcgat aacggctact cccttcgtgt tgttgatgaa
    18841 tctgaccaga cctcagcaga atgcacgcca ccatttgcta cccttaccgg catacgctgc
    18901 agtaccgcac atatcacgga aacggacaac gcctggttgt actcgctgtc acaccagacc
    18961 agtgacttcg gtgaatcaga atggattcat tttacaggta gcggatatct gttacgtacc
    19021 gatgcgtggt catacccggt tctgcggctt aaacgcctgg ggctgtcaaa aacgttccgt
    19081 cgtctggtcg tcacactcat ccggcgttat ggcgtcagtc tcattcatct ggatgccagc
    19141 gctgaatgcc tgccgggttt acccactttc gactggtaac caggaacaac atgaaatcat
    19201 taaccacgga aacagcgctg gatattctga ttgcgtggct gcaggacaat atcgactgcg
    19261 gatcgggaat tatctttgac aacgatgagg ataaaacgga ttcagcagca ctgttgccct
    19321 gtatcgaaca ggcccgggag gatgtccgta ccctgcgtca tctgcagctt ctgcaccaga
    19381 accggtgaag ctcactcacc agacttcatt cctctgacgc cagcctgcac agggctggcg
    19441 ttttcattta tctgcaaaaa ggaatatcga ttatgtcaga tatcacaatc tcccgtccgg
    19501 aagtggtcaa cgggcatacg gacgttatct gctcaaccag cattcgccac attctggctg
    19561 tacgaaagag tacactgttg caaatcgaca cacttatccg acaactggct gaaatctcgg
    19621 tgctgacgga aagcatcgga ggtaaaactg ccccggactg ggccatgaaa caggattttc
    19681 gctgcggctg ctggctgatg gagaaacccg aaacagcaat gaaagccatt acccggaatc
    19741 ttgatcgcga aatctggcgt gacctgatgc aacgttccgg gatgctgtca ttaatggata
    19801 cgcaggcccg tgatacatgg taccggtcac tggagtacga taattttccg gaaatcagcg
    19861 aagcgaacat tctgagcaca tttgaacaac tgcaccagaa taaggatgag gtgtttgagc
    19921 gaggggtgat caacgtcttc agagggctga actggaatta caaaaccaat tgcccctgta
    19981 aatttggcag taaaattatc gtcaacaatc tggtgagatg ggaccggtgg ggataatgat
    20041 atggtcaccc ccaccttaac agcagtacgc tgttaaggta aatcccttta acggagagac
    20101 catcatgcag aacatcacac ttattggtat cgatctcgga aagcactcct tccacattca
    20161 ttgtcaagac aagtctggta aggctcttct gcgtaaaaaa tttacccgta ctaaactgat
    20221 gcagtttctg gcgacatgtc cgccctcagt tgttgtaatg gaagcctgtg cgggtgcaca
    20281 ctttatggct cgctgtatct ctgattttgg gcatgaaact aagctgattt ctccacagtt
    20341 cgttcgcccg tttgtcaaaa gtaataagaa tgattttgtt gatgcagaag ccatatgcga
    20401 agcggcttca cgaccatcaa tgcgttttgt ccagccaaga actgagaccc agcaggccat
    20461 gagggcgtta caccgggtca gagaatcgct catcagagat aaggtgaaaa caaccaacca
    20521 gatccacggt ttcttgctgg aatttggaat cagcctgccg ataggtgatg ctgtaataaa
    20581 acggttgtct ctcgtgttgg ctgaacacga aatccccgaa tatctgagcc gccttttggt
    20641 gaggttgcat acccactact tttaccttgt tgagcaaata gcagagctgg aatcagaatt
    20701 aagtcagtca atcaacgctg atgataccgc tcagcgcatt atgacaattc ctggtgttgg
    20761 tccgattact gccagcttac tatcgtcgca acttggtgac gggaaacagt tctcatgcag
    20821 cagggatttt gcagcttcca caggacttgt tccccggcag tatagcactg gaggaaaatc
    20881 gactctgttg ggtatcagta agcgaggtga taaaaattta cgccggttac tggttcagtg
    20941 tgcccgctca ttcataatgc gacttgaacg caagcatgga aaacttgctg agtgggttcg
    21001 ggagcaactc aacaagaagc attcgaatgt ggttacctgc gctctggcaa ataaactggc
    21061 tcgaattgcc tgggcaatca ctactcagaa aaaagaatat caggcctgat atagatgctg
    21121 atataactca attagtttat tttaaacaat aagatacaag ttacctttct ggttttgcga
    21181 agactgatta ttgatgacat gaacggcaaa tcgacctgat taaaaaccta acagatgaaa
    21241 tggctcactg aagccgaggg gatttttagg attatcaggt gcggaactca tcggggcgcg
    21301 ggcatccttg cccacagaga cgccggatag atttaagcaa gccaaccatt aatcaaaaat
    21361 cagtgttgca aaaacggggg tgaccataga tttcatctta tcaccggaca acaggctgac
    21421 cgactcgccg atctggaaag aatgttgcac ctgttcagcg gtaaaccaat ccccgacaac
    21481 cggggaaaca tcacccttcg tctggatgat cacattcagt ctgttcaggg taaagagagc
    21541 tatgaagatg agatgttcag catcagatac tttaagaagg gctccgcgca tatcacgttc
    21601 agaaaaccag aactggttga cagactgaat gatattatcg caaaacacta tcctgatatg
    21661 ctggcagtat agctttgcca gtatgctcaa agatcggaaa ttgtccgata agatatgatc
    21721 gcggggcata taaaccggtt tcagatggca tacccgatgg catactgtct ggaaggaaaa
    21781 ataaaaaagc gcatgttaaa caataacatg cacttataat ctggcggtga gggggggatt
    21841 cgaacccccg atacgttgcc gtatacacac tttccaggcg tgctccttca gccactcgga
    21901 cacctcacca aattgtcgtt cctgtcttgc tggaacgggc gctaatttag ggaaatcatg
    21961 acctgaggtc aacaaacttt ttgaaaaaat cgcgcgttta ttcaaacatt caatcaatgt
    22021 gtggttttaa taagcgaaat ctgctttttt tgccaccgac cacggatttg ttatgctggt
    22081 ggcctttgta gatcataacg ataagtgcga ataaatttcg cacaacgctt ttcgggagtc
    22141 agtatggata tcatctttta tcacccaacg ttcgataccc aatggtggat tgaggcactg
    22201 cgcaaagcta ttcctcaggc aagagtcagg gcatggaaaa gcggagataa tgactctgct
    22261 gattatgttt tagtctggca tcctcctgtt gaaatgctgg cagggcgcga tcttaaagcg
    22321 gtgttcgcac tcggggccgg tgttgattct attttgagca agctacaggc acaccctgaa
    22381 atgctgaacc cttctgttcc actttttcgc ctggaagata ccggtatggg tgagcaaatg
    22441 caggaatatg ctgtcagtca ggtgctgcat tggtttcgac gttttgacga ttatcgcatc
    22501 cagcaaaata gttcgcattg gcaaccgctg cctgaatatc atcgggaaga ttttaccatc
    22561 ggcattttgg gcgcaggcgt actgggctgt aaagttgctc agagtctgca aacctagcgc
    22621 tttccgctgc gttgctggag tcgaacccgt aaatcgtggc ctggcgtgca aagctttgcc
    22681 ggacgggaag aactgtctgc atttctgagc caatgtcggg tattgattaa tttgttaccg
    22741 aatacccctg aaaccgtcgg cattattaat caacaattac tcgaaaaatt acctgatggc
    22801 gcgtatctcc tcaacctggc gcgtggtgtt catgttgtgg aagatgacct gctcgcggcg
    22861 ctggatagcg ggaaagttaa aggcgcaatg ctggatgttt ttaatcgtga acccttaccg
    22921 cctgaaagtc cgctctggca acatccacgc gtgacgataa caccacatgt cgccgcgatt
    22981 acccgtcccg ctgaagctgt ggagtacatt tctcgcacta ttgcccagct cgaaaaaggg
    23041 gagagggtct gcgggcaagt cgaccgcgca cgtggctact aataaagcat caggattcct
    23101 gctatccttg gcgggaattg aatacaggag agagttatgt atcccgtcga ccttcatatg
    23161 cataccgttg ccagcacaca tgcatatagc acattgagtg attacattgc ccaggccaaa
    23221 caaaagggca ttaaactttt tgcgatcacc gatcatggcc cggatatgga agatgcgccg
    23281 catcactggc acttcattaa catgcgtatc tggccgcgag tggttgatgg ggtagggatc
    23341 ctgcgcggca ttgaagctaa cattaaaaat gttgatggtg aaattgactg cagcggtaaa
    23401 atgtttgact cgctggatct gattattgcc ggttttcatg agccggtttt tgcgccacat
    23461 gacaaagcga ccaatacaca agcgatgatc tccactatcg ccagcggcaa tgtgcatata
    23521 ataagccatc ccggaaatcc gaaatatgaa atagatgtta aagccgttgc tgaagcagcc
    23581 gcgaaacatc aggtggcgct ggaaatcaat aattcctcat ttttacactc acgtaagggc
    23641 agtgaagaca actgtcgtgc ggtagctgca gcggtacgtg atgctggagg ttgggtggca
    23701 ttaggctcgg attctcacac cgcgtttacc atgggggaat ttgaagagtg tcttaaaatc
    23761 ctcgacgcgg tagattttcc gctagagcgc attttgaatg tttctccgcg ccgcttactg
    23821 aacttccttg aatctcgcgg tatggcaccg attgcggaat ttgcagacct ttaattacta
    23881 gtaatggaaa tatattaatg aacgagtttt ctatcctctg tcgtgtgctg ggttcgctct
    23941 attaccgcca accgcaagat cctttactgg tgccgctgtt tactctgatt cgtgagggga
    24001 aactggctgc gaactggcca ctggagcagg atgagttact gacgcgttta cagaaaagct
    24061 gtgatatgac gcaagtctct gccgattaca atgcgttgtt tatcggcgat gaatgtgccg
    24121 tgccgccata tcgtagcgca tgggttgagg acgcaacgga agcggaagtg cgcgcttttc
    24181 tttccgaacg agggatgcca ctggcggata cgccagccga tcacatcggc acattgctgc
    24241 ttgcggcttc ctggctggaa gatcagtcaa cggaagatga gagcgaagca ctggaaacac
    24301 tgttcagtga gtatctgtta ccctggtgtg gtgcgttcct cggcaaagtg gaggcccatg
    24361 caaccacgcc tttctggcgc accatggcac cgctaacccg tgatgccatt agtgcaatgt
    24421 gggacgagct ggaagaagat tctgaagagt aaatgtgatc atcgtcactc ataactgcaa
    24481 cagataattt gttgttgcat aaaatgtgtg ctcgatctca ttcatggccg cgttttctgc
    24541 tatcatgcgc ggcatgaaca tacttctctc tattgcaatc acaacaggca ttctctccgg
    24601 tatctgggga tgggtggctg tttccctggg cttactaagc tgggcgggct tcctaggctg
    24661 tacggcctac tttgcttgcc cgcaaggtgg gctgaaaggg ctggcgatct ccgctgcaac
    24721 cctgcttagt ggcgtggtgt gggcgatggt cattatttac ggtagtgcac tggcaccaca
    24781 tctggaaatt ctcggttatg tcataaccgg tattgtcgcg tttctgatgt gtattcaggc
    24841 caaacagctg ctgctttcat ttgttccggg gacgtttata ggcgcatgcg cgacgtttgc
    24901 cgggcagggt gactggaaac tggtgttacc ttcgctggcg cttgggctgg tatttggtta
    24961 cgcaatgaaa aacagcggcc tgtggctggc ggcgcgtagt gcaaagacgg ctcaccgtga
    25021 gcaggaaatc aaaaataaag cgtgaggggc actcacgctt tcgcttaaac agtaaatgcc
    25081 ggattattaa ttccggcatt tttatctgtc aggattccgg tggaaccgac atatggcggt
    25141 atttcaccag aatgtcattc tgccgttctg ctttatttta caaatcccac agaccacggt
    25201 cgataccatc attaatcagg aaaatgaccc ctgtttcgat agccgacatc agacacagca
    25261 taacgggttc gtttgaggtg taacccactt ccccttccag caagcgctgg tagtcaataa
    25321 agcggaaaac cccggcctga acttcatagg aaagtatcgt cttactggtg ttcaccgagg
    25381 agaggatctc accggtactc acattgacga cacgcaggtt cacggcaatc tgatcgagct
    25441 ggtattgcgt gtctgcaccg atcccaaaat atcttgcccc aacaccgccc gatttaacgt
    25501 tgctttcata accgataatc gaaccttcaa ccataatatt tgccgccgtt aaagattgca
    25561 gcgggattcg gttattaatg gcaaccgtgc cgttttcttg tgccgcacga ataaccttgc
    25621 gctcgttaag caggttttgt aagccctggc gctttgcgga acagcagtgg agaagttact
    25681 tgccgggtag ggtttaaatt gcccggtttc gtcctgaatg ttgtataccg aaacaaagat
    25741 cttacccgtc ggcgctggca gatgggtcaa atctttgtag ctctgagcac gaggcattaa
    25801 tgtcggtctg gcggcttctt taggcggggc ggttaagcat ccgctcagta acatgacggc
    25861 aaccaaaaga aataagcgct gcatgattat tttccttatg aagctggggc ttaaaaatcg
    25921 gttgagttat tttgtaaacc cgaaacctgg atggtcgagg tttgtccggt tttacgatct
    25981 gtcacgttca actgcaattg accatcgcgg ttagcaatat caacaatata atcgttggtc
    26041 accatgcggc ccggtttacc ggtattaata ttcgacagta gcccacctaa aatttgtgac
    26101 tggatggcct gagtaaagtt atctaacgct gagggtgttt caataccaaa gtcatcgtta
    26161 tagctcggat ctttataaga gttttgggcc tgagcgctat ttaataaaaa agcgccatta
    26221 tttgggttac caccaaagtt tggattacgg aactggaaag tcatggttcc agcccaactt
    26281 aatggcgaaa taagcatgag tagaactact gcatgtttga cacgcattgc agcctccgaa
    26341 caatttttta tttagaattc atcatgcgcc aaatcgtccg tacttaataa cgcctgattt
    26401 atctggcgac gatttagtgc ttcttcagtt tgaatcagtg caaagacgac agttttctcg
    26461 aagtctcttt tcaacgggaa taaaaaagtc tggaaaataa catcctgatt gaccgttatt
    26521 gtgatccagc ttccccatcg tgcactgggc ctttcattaa tcgttaagtt acccgtatag
    26581 tcactttccc atttatcact aaaggctcgg taaaaatcat ggccaataga tgaaacagta
    26641 tggtcagtta acaatcccgg gacttctacc tcaacggcgt gaagattccc tgcggcgaac
    26701 agaaattctg ccgccacaat ccagcgtaaa taacgtttca tggctttatc gcctgaggtt
    26761 atcgtttgcc caggaaaccg cttgtgtccg gttttttacg gctatcttct tgaaaagatt
    26821 ataaagatgc gttttaaccg tattttcgct gatgaacaac gaacgagcga tctcgttatt
    26881 agacgcgccg atacgcagct tattcaggat ctctttttcc cgatgagtaa ggagggctga
    26941 ttccgtgctg ttataacgat agttaccaga atgtgtaatc aggtaactgg caagcttttg
    27001 cgtaaagtag cattcgccac gcaggacgcc ttgcaaccca ttgacaacac gttcttgatc
    27061 ctccatggca taaaaaacgc cgttgatatg aggccagttt tcaatgtcgc ggtacgggta
    27121 atcttcaggc gtatttagca acaatatttt gatattgttg tttttcctgc tcaaagtatc
    27181 ctgccaataa tggataagct ttttatccgc ttccatcata tccagaagaa taatagagcc
    27241 tgaagagata tcgtccagag aacgttgaat attatgtaat tttcctgtaa ttgctagcga
    27301 ttgtttaagg tactgcaaga gagctgtcgc ctgcaaagaa ggtttagtga tcaacaataa
    27361 tgtatgacca tgaatactat ggacttcatt aaacatgatg aaaccccact tttttattga
    27421 tcgcacacct gacagctgcc tctaaaatag aagcaccaga agtactgaca gatgttgcac
    27481 tgctgtgtgt agtaataaat cagccctaaa tgggtaaaat ataaaactaa tggattacat
    27541 ctgatttcaa tctagccatt acaaatctta aatcaagtgt taaacatgta acaaaatgta
    27601 actcattata ttaaaatgtt aacattaagg ttttattaag tttagaaatg atagaaaagt
    27661 tgtacatttg gtttttattg cacaatttta aaaaatcata caaatggtgc taacttacta
    27721 ataatacata taaaaatatt tcggtgtagt cctttcgtca tgtaaaacgt ttttgttttt
    27781 tctccacacc tccgtggaca attttttact gcaaaaagac gaggtttgtc acggcttgtg
    27841 cgcaagacat atcgcagcaa tcagcgacgg gcaagaagaa tgactgtctg gtgatttttg
    27901 atagcggaaa acggagattt aagagaaaac aaaatatttt ttgcgtagat aacagcgtat
    27961 ttacgcgggt tttaatactt tggtatgaac aaaaaaagaa aaatacaacg cgcgggtgag
    28021 ttattaaaaa tatttccgca gacatacttt ccatcgtaac gcagcgttaa caaaatacag
    28081 gttgcgttaa caaccaagtt gaaatgattt aatttcttaa atgtacgacc aggtccaggg
    28141 tgacaacatg aaaaacaaat tgttatttat gatgttaaca atactgggtg cgcctgggat
    28201 tgcagccgca gcaggttatg atttagctaa ttcagaatat aacttcgcgg taaatgaatt
    28261 gagtaagtct tcatttaatc aggcagccat aattggtcaa gctgggacta ataatagtgc
    28321 tcagttacgg cagggaggct caaaactttt ggcggttgtt gcgcaagaag gtagtagcaa
    28381 tcgggcaaag attgaccaga caggagatta taaccttgca tatattgatc aggcgggcag
    28441 tgccaacgat gccagtattt cgcaaggtgc ttatggtaat actgcgatga ttatccagaa
    28501 aggttctggt aataaagcaa atattacaca gtatggtact caaaaaacgg caattgtagt
    28561 gcagagacag tcgcaaatgg caattcgcgt gacacaacgt taattaccat tcgactttta
    28621 aatcaatccg atgggggttt tacatgaaac ttttaaaagt agcagcaatt gcagcaatcg
    28681 tattctccgg tagcgctctg gcaggtgttg ttcctcagta cggcggcggc ggtaaccacg
    28741 gtggtggcg
//
LOCUS       DAJVPP010000050        28642 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVPP010000050 DAJVPP010000000
VERSION     DAJVPP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.050"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1957)
                     /gene="yccS"
                     /locus_tag="OQM34_002148"
     CDS             complement(<1..1957)
                     /gene="yccS"
                     /locus_tag="OQM34_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1452587.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDA"
     gene            complement(1967..2413)
                     /locus_tag="OQM34_002149"
     CDS             complement(1967..2413)
                     /locus_tag="OQM34_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1452588.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2536..4590
                     /gene="helD"
                     /locus_tag="OQM34_002150"
     CDS             2536..4590
                     /gene="helD"
                     /locus_tag="OQM34_002150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1452589.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4622..5080)
                     /gene="mgsA"
                     /locus_tag="OQM34_002151"
     CDS             complement(4622..5080)
                     /gene="mgsA"
                     /locus_tag="OQM34_002151"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1452590.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5176..5838)
                     /locus_tag="OQM34_002152"
     CDS             complement(5176..5838)
                     /locus_tag="OQM34_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1452591.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6011..6424
                     /locus_tag="OQM34_002153"
     CDS             6011..6424
                     /locus_tag="OQM34_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1452592.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6469..6786)
                     /gene="hspQ"
                     /locus_tag="OQM34_002154"
     CDS             complement(6469..6786)
                     /gene="hspQ"
                     /locus_tag="OQM34_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1452593.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(6844..8034)
                     /gene="rlmI"
                     /locus_tag="OQM34_002155"
     CDS             complement(6844..8034)
                     /gene="rlmI"
                     /locus_tag="OQM34_002155"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCS1452594.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8129..8407
                     /gene="yccX"
                     /locus_tag="OQM34_002156"
     CDS             8129..8407
                     /gene="yccX"
                     /locus_tag="OQM34_002156"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1452595.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8404..8733)
                     /gene="tusE"
                     /locus_tag="OQM34_002157"
     CDS             complement(8404..8733)
                     /gene="tusE"
                     /locus_tag="OQM34_002157"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1452596.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8824..9483)
                     /gene="yccA"
                     /locus_tag="OQM34_002158"
     CDS             complement(8824..9483)
                     /gene="yccA"
                     /locus_tag="OQM34_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1452597.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9690..9777)
                     /locus_tag="OQM34_002159"
     tRNA            complement(9690..9777)
                     /locus_tag="OQM34_002159"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9741..9743),aa:Ser,seq:tga)
     gene            10203..11321
                     /gene="hyaA"
                     /locus_tag="OQM34_002160"
     CDS             10203..11321
                     /gene="hyaA"
                     /locus_tag="OQM34_002160"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1452598.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLIALDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQSTETEHQPGNEDKQA"
     gene            11318..13111
                     /gene="hyaB"
                     /locus_tag="OQM34_002161"
     CDS             11318..13111
                     /gene="hyaB"
                     /locus_tag="OQM34_002161"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCS1452599.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13130..13837
                     /gene="hyaC"
                     /locus_tag="OQM34_002162"
     CDS             13130..13837
                     /gene="hyaC"
                     /locus_tag="OQM34_002162"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCS1452600.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            13834..14421
                     /locus_tag="OQM34_002163"
     CDS             13834..14421
                     /locus_tag="OQM34_002163"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1452601.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14418..14816
                     /gene="hyaE"
                     /locus_tag="OQM34_002164"
     CDS             14418..14816
                     /gene="hyaE"
                     /locus_tag="OQM34_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1452602.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14813..15670
                     /locus_tag="OQM34_002165"
     CDS             14813..15670
                     /locus_tag="OQM34_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1452603.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15804..17348
                     /gene="appC"
                     /locus_tag="OQM34_002166"
     CDS             15804..17348
                     /gene="appC"
                     /locus_tag="OQM34_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1452604.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMVLWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17360..18496
                     /gene="appB"
                     /locus_tag="OQM34_002167"
     CDS             17360..18496
                     /gene="appB"
                     /locus_tag="OQM34_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1452605.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18508..18600
                     /gene="cbdX"
                     /locus_tag="OQM34_002168"
     CDS             18508..18600
                     /gene="cbdX"
                     /locus_tag="OQM34_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1452606.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18680..19978
                     /gene="appA"
                     /locus_tag="OQM34_002169"
     CDS             18680..19978
                     /gene="appA"
                     /locus_tag="OQM34_002169"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1452607.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20093..22273)
                     /gene="etk"
                     /locus_tag="OQM34_002170"
     CDS             complement(20093..22273)
                     /gene="etk"
                     /locus_tag="OQM34_002170"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS1452608.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22293..22739)
                     /gene="etp"
                     /locus_tag="OQM34_002171"
                     /gene_synonym="yccY"
     CDS             complement(22293..22739)
                     /gene="etp"
                     /locus_tag="OQM34_002171"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1452609.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22727..23866)
                     /locus_tag="OQM34_002172"
     CDS             complement(22727..23866)
                     /locus_tag="OQM34_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS1452610.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(23912..26008)
                     /locus_tag="OQM34_002173"
     CDS             complement(23912..26008)
                     /locus_tag="OQM34_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1452611.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QPYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26008..26754)
                     /locus_tag="OQM34_002174"
     CDS             complement(26008..26754)
                     /locus_tag="OQM34_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1452612.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQF
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26751..27395)
                     /locus_tag="OQM34_002175"
     CDS             complement(26751..27395)
                     /locus_tag="OQM34_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1452613.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27502..27807)
                     /locus_tag="OQM34_002176"
     CDS             complement(27502..27807)
                     /locus_tag="OQM34_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452614.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28249..28461)
                     /gene="cspH"
                     /locus_tag="OQM34_002177"
     CDS             complement(28249..28461)
                     /gene="cspH"
                     /locus_tag="OQM34_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCS1452615.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         6934 a         7263 c         7545 g         6900 t
ORIGIN      
        1 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
       61 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
      121 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
      181 ataccaccga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
      241 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
      301 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcaggccaga
      361 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
      421 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
      481 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
      541 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
      601 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
      661 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
      721 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
      781 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
      841 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
      901 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
      961 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
     1021 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
     1081 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
     1141 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
     1201 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
     1261 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
     1321 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
     1381 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
     1441 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
     1501 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
     1561 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
     1621 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
     1681 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
     1741 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
     1801 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
     1861 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccagg
     1921 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
     1981 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
     2041 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
     2101 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
     2161 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
     2221 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
     2281 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
     2341 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
     2401 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
     2461 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
     2521 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac
     2581 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
     2641 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
     2701 gagctggaat ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac agaatggggc
     2761 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
     2821 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
     2881 gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat ccgccaggct
     2941 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
     3001 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
     3061 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
     3121 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
     3181 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
     3241 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
     3301 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
     3361 catgcgttgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
     3421 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
     3481 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
     3541 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
     3601 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
     3661 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
     3721 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
     3781 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
     3841 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
     3901 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
     3961 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
     4021 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
     4081 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
     4141 aaagccgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
     4201 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
     4261 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
     4321 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtggt
     4381 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
     4441 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
     4501 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
     4561 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
     4621 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
     4681 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
     4741 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
     4801 ttagtggatc ccagaagaat atcaatacat caattttccc ttctgagatc aatgcgccaa
     4861 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
     4921 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
     4981 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
     5041 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
     5101 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
     5161 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
     5221 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
     5281 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
     5341 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
     5401 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
     5461 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
     5521 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
     5581 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
     5641 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
     5701 accgcgcagc agagagctgg aaaccttctt gccgtcgagt accagaagat cgacatcggt
     5761 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
     5821 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
     5881 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
     5941 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
     6001 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
     6061 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
     6121 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
     6181 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
     6241 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
     6301 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
     6361 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
     6421 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
     6481 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
     6541 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
     6601 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
     6661 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
     6721 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
     6781 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
     6841 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
     6901 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
     6961 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
     7021 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
     7081 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
     7141 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
     7201 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
     7261 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
     7321 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
     7381 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
     7441 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg
     7501 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
     7561 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
     7621 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
     7681 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
     7741 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
     7801 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
     7861 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
     7921 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
     7981 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
     8041 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
     8101 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
     8161 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
     8221 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
     8281 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
     8341 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
     8401 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
     8461 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
     8521 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
     8581 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
     8641 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
     8701 cgtttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
     8761 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
     8821 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
     8881 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
     8941 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
     9001 atgcagagca ggcagttgca ggaagatatt agcaatcata ccaatcagta ccaccacaat
     9061 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
     9121 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
     9181 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
     9241 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
     9301 accatacata cccaccagag tcagaatcag acccggagat ggcagcatca gcaccgtact
     9361 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
     9421 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
     9481 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
     9541 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
     9601 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
     9661 gtagcgttta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
     9721 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
     9781 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
     9841 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagtttgatc atttcatcac
     9901 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
     9961 aagaaaaata ggtccatttt tatcgctaaa agataaatcc acacagtttg tattgttttg
    10021 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    10081 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    10141 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    10201 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    10261 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    10321 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    10381 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg gcgaaggacg
    10441 tcatcctttc cctgattgcc ctcgattacg acgataccct gatggctgcc gccggaaccc
    10501 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    10561 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    10621 ttattgaaaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    10681 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    10741 acaaagtcat caccgacaaa cccattatca aagtacctgg ctgcccgccg atcccggatg
    10801 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    10861 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    10921 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    10981 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    11041 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    11101 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    11161 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    11221 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caatctacag
    11281 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    11341 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    11401 aggccacatg cgctgtgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    11461 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    11521 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    11581 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    11641 cattatgctg gcaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    11701 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    11761 ggcgcaaagt ctctcctctt ggccgaaatc atcccctggc tatttcttcg acgtacaaaa
    11821 tcgcctgaag aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg
    11881 gcacccgcag tacaaactgc cgccagaagc caacctgatg ggctttgccc actatctcga
    11941 agctctcgat ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc
    12001 gcatccaaac tggattgtcg gtgggatgcc ttgcgccatt aacattgacg aaagcggcgc
    12061 ggtcggggcg gtcaatatgg aacgcctgaa cctggtgcag tcgattatca cccgcacggc
    12121 agacttcatt aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc
    12181 gtggagcgaa atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcattccc
    12241 ggatattgcc aacgactttg gtgagaaaag tctgctgatg cctggcggcg cggtgattaa
    12301 cggcgacttc aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt
    12361 tgtcgatcac gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg
    12421 cattaccgac ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca
    12481 gctgaatgaa caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat
    12541 ggaagtgggg ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt
    12601 tgagtcggtc gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac
    12661 gttgggccgc attttgtgcc gcgcgcacga agcgcagtgg gccgcaggta agttgcagta
    12721 tttcttcgac aagctgatga ccaacctgaa aaacggcaat ctcgccactg cttccactga
    12781 aaaatgggaa cctgcaacct ggccgacaga gtgccgtggc gtcggcttta ccgaagcgcc
    12841 gcgcggagcg ttaggccact gggccgccat tcgcgatggc aagattgatc tctaccagtg
    12901 cgtggtgccg accacctgga acgccagtcc gcgcgatccc aaagggcaga ttggcgctta
    12961 tgaagcggcg ctgatgaaca ccaaaatggc gatccccgag caaccgctgg agatactgcg
    13021 tactctgcac agctttgatc cgtgcctcgc ctgttcaact catgtgctgg gcgacgacgg
    13081 tagcgagctg atctccgtgc aggtgcgtta acagcgaagg agaatcatca tgcaacagaa
    13141 aagcgacaac gttgtcagcc actatgtctt tgaagcacca gttcgtatct ggcactggct
    13201 gacggtgtta tgcatggcgg tgttgatggt caccggttac tttatcggca agccgctacc
    13261 ttccgtcagt ggcgaggcga cgtatctgtt ctatatgggt tacatcaggt taattcactt
    13321 cagcgccggg atgattttta ccgtggtctt gctgatgcgg atctactggg cttttgttgg
    13381 caatcgttac tcccgcgagc tgtttatcgt gccggtatgg cgtaaaagct ggtggcaggg
    13441 cgtgtggtat gaaatccgct ggtatctgtt tctggcaaaa cgtccgagtg ccgatatagg
    13501 ccataacccc atcgcccagg cggcgatgtt cggctatttc ctgatgtcgg tctttatgat
    13561 catcaccggt tttgcgctgt acagcgaaca cagccagtac gctatttttg cgccgttccg
    13621 ttatgtggtg gaatttttct actggacggg tggcaactca atggacattc acagctggca
    13681 tcggctgggg atgtggctga ttggggcgtt tgtgatcggt catgtctaca tggcgctgcg
    13741 tgaagacatc atgtccgacg acacggtgat ctccaccatg gtcaacggct accgtagcca
    13801 caaatttggc aaaataagta acaaggagcg ttcatgagcg agcaacgcgt ggtggtcatg
    13861 gggctgggca acctgctgtg ggccgatgaa ggcttcggcg tgcgggtggc ggaacggctg
    13921 tatgcccatt accactggcc cgagtatgtg gagattgtcg atggcggtac tcagggactg
    13981 aacttgctgg ggtatgtcga aagcgccagc catctgttga ttctcgatgc cattgactac
    14041 gggctggaac ctggaacgct gcgaacctat gccggagaac gcattccggc ctatctcagc
    14101 gcgaagaaaa tgagcctgca tcagaacagt ttctccgaag tgctggcgct ggcagatatc
    14161 cgcggacatc tgccagcaca tattgccctc gtcgggctgc aacccgcgat gctcgacgac
    14221 tacggcggca gcctgagcga actggcacgg gagcaactgc ccgctgcgga acaatcggcg
    14281 ctggcgcagt tggctgcgtg gggaagtgtg ccgcaaccgg ctaatgaatc gcgttgtctc
    14341 aattatgact gtctgtcgat ggaaaattac gaaggcgttc gcttgcgcca gtaccggatg
    14401 acacaggagg agcagggatg agcaacgaca cgccatttga tgcgttgtgg caacgaatgc
    14461 tggcgcgcgg ctggacgccg gtcagtgaat cccgtcttga cgactggctt acgcaagcgc
    14521 cagacggcgt ggtgttatta agcagtgacc cgaaacgcac gccagaggtc agcgataatc
    14581 cggtaatgat tggcgaatta ctgcacgagt ttcccgacta tacatggcag gtggcgattg
    14641 ctgaccttga gcagagcgaa gccatcggcg atcgctttgg cgcctttcgc tttcctgcca
    14701 ctttagtgtt taccggcgga aactatcgcg gcgtgctgaa tggtattcac ccgtgggcgg
    14761 aactgataaa cctgatgcgc gggcttgtcg aaccgcagca ggagcgtgcc tcatgagcga
    14821 aacttttttc catctgctgg ggccaggaac gcaaccgaac gatgacagtt tcagcatgaa
    14881 tccactgccg atcacctgtc aggtgaatga tgaaccgagt atggcggcct tggagcaatg
    14941 tgctcacagc ccgcaggtga ttgcgctgtt aaacgagtta caacatcaac taagcgaacg
    15001 ccagccgccg ttgggcgagg tgctggcagt cgatctgtta aatctcaacg ccgacgatcg
    15061 tcactttatc aatacgcttc tcggggaagg ggaagtgtca gtgcgcattc agcaggctga
    15121 cgacagtaaa agtgaaatac aggaggcgat cttctgcgga ttatggcggg tgcgcagacg
    15181 tcgcggcgaa aagttgctgg aggacaaact ggaggctggc tgcgcgccgc tggcgttgtg
    15241 gcaggcggca acgcaaaatc tcttgccgac agattcgctg ttaccgccgc ccattgatgg
    15301 cctgatgaat ggcctaccgt tggcgcatga gttactggca catgtacgta accccgacgc
    15361 gcagccgcac agcattaatc tgacgcaatt acccatcagc gaggctgatc ggctttttct
    15421 ctcgcgtctc tgtgggccgg gaaatattca gattcgtacc attggctatg gcgagagcta
    15481 tatcaacgcc acggggttac gccatgtctg gcatttacgc tgtacggaca ccttaaaagg
    15541 cccgttactg gaaagttatg aaatctgccc aataccggaa gtggtgctgg cagcgccaga
    15601 agatttggtc gactctgcgc agcggcttag cgaggtatgt cagtggctgg cggaagctgc
    15661 accgacgtaa aaagacggta agtatcgctt tcagtcttat gaatatcgca atcggcgaat
    15721 acctctggtc gtagagtttc aggataaaga gggagatcta acattatcgg gttatttttc
    15781 tctcttcgcc tacaggagtg cgcatgtggg atgtcattga tttatcgcgc tggcagtttg
    15841 ctctgaccgc gctgtatcac tttttatttg taccccttac cctggggctg atttttttgc
    15901 tggctatcat ggaaaccatt tacgtggtca ccggcaaaac aatctaccgc gatatgacgc
    15961 gcttctgggg taagctcttc ggtatcaatt ttgctcttgg cgtggctacc ggcctgacca
    16021 tggagtttca gtttggtact aactggtcat tctattccaa ctatgtgggc gatatttttg
    16081 gcgcaccact ggcgatggaa gcattaatgg ccttcttcct cgaatccacc tttgtcgggc
    16141 tgttcttctt cggctggcaa cggctgaata aataccagca cctgctggtg acgtggctgg
    16201 tggcgttcgg ttcaaatctc tctgcattgt ggatattgaa tgccaacggt tggatgcaat
    16261 acccgaccgg tgcgcatttt gatatcgaca ccctgcgtat ggagatgacc agcttcagcg
    16321 agctggtctt taacccggtc agccaggtga aatttgtgca caccgtcatg gcgggctacg
    16381 tgaccggggc catgtttatt atggcgatca gcgcctggta tttactgcgc ggacgggagc
    16441 gcgatgtcgc attacgctcg tttgccatcg gttccgtctt cggtactctg gcgattatcg
    16501 gtaccctgca actcggagac agttctgcgt atgaagtcgc gcaagtacaa ccggtaaaac
    16561 tggcggcgat ggaaggggag tggcaaacgg aacctgctcc tgcaccgttc catgtggttg
    16621 cctggccgga acaggatcaa gagcgtaacg cctttgccat caaaattccc gcgctgctag
    16681 ggatcctcgc cactcactca ttagataaac ccgtgccggg tctgaagaat ttgatggctg
    16741 aaacctaccc acgcttgcaa cgcggacgta tggcctggct gttaatgcag gaaatatctc
    16801 aaggcaatcg tgagccgcat gtgttgcagg cattccggga gctggaaggc gaccttggtt
    16861 acggcatgtt gctctcccgc tatgcgccgg atatgaatca tgtcacagcc gcacagtacc
    16921 aggcggcgat gcgtggcgcg atacctcagg ttgcgccggt attctggagt ttccgcatca
    16981 tggtgggctg tggttccctg ctgctactgg tgatgctgat tgcgcttgtc cagacgctgc
    17041 gtggcaaaat cgaccagcat cgctgggtgc tgaaaatggt gctctggagt ttgccgttgc
    17101 cgtggattgc gattgaagcc gggtggttta tgaccgagtt tggtcgtcag ccgtgggcga
    17161 tacaggacat cttaccgaca tactccgcgc actccgcttt aaccacagga caactggctt
    17221 tctcactgat catgatcgta gggctttaca ccctgttctt aatcgccgaa gtctacctga
    17281 tgcagaaata tgcccgtctg gggccgagcg cgatgcagag tgaacaaccg acgcagcaac
    17341 aggggtaaag gagaaaatca tgtttgatta tgaaacattg cgcttcatct ggtggctgct
    17401 gattggcgtg atcctggtgg tctttatgat ctccgacgga tttgacatgg ggatcggctg
    17461 tttgctgccg ctggtggcgc gtaatgatga tgaacgccgg atagtgatta atagcgttgg
    17521 tgcgcactgg gaaggcaatc aggtctggtt gatcctcgca ggtggggcat tatttgccgc
    17581 ctggcccaga gtgtatgcag cggcgttttc cggcttttat gtggcgatga tcctggtgct
    17641 gtgctcgctg ttcttccgtc cgctggcctt tgattatcgc ggaaaaattg ccgatgcacg
    17701 ctggcgtaaa atgtgggatg ccggtctggt catcggcagt ctggtgccgc cggtagtctt
    17761 cggtatcgcc ttcggcaact tgttgctcgg cgtgccgttt gccttcacac cgcaattacg
    17821 cgtggagtat ctcggcagct tctggcaact gctgacgcca ttccctttat tgtgtggttt
    17881 gctcagcctt gggatggtga ttttgcaagg tggcgtctgg ttacaactga aaactgttgg
    17941 tgtgattcat ctgcgttcac agctggcgac taaacgcgct gcactgttgg tgatgctgtg
    18001 ctttttgctg gcaggttact ggctgtgggt cggtattgat ggctttgtac tgctcgccca
    18061 ggatgctaac ggtccttcca atccgttaat gaaactggtg gcagtgctac ctggtgcctg
    18121 gatgaataat ttcgtcgaat cgcccgtttt gtggatcttc ccgctgctgg ggttcttctg
    18181 cccattgctg acggtgatgg cgatttatcg tggtcgcccg ggttggggat ttttgatggc
    18241 atcattgatg caatttggcg tgattttcac ggcaggcatc acgctgttcc cctttgtcat
    18301 gccgtcaagt gtgagtccaa tctccagcct gacgttgtgg gacagcactt ccagtcagct
    18361 gacgctgagc attatgttgg taatcgtgct gatatttttg cccattgtgt tgctctacac
    18421 tctctggagc tactacaaaa tgtggggacg catgacaaca gaaactctcc gccgtaacga
    18481 aaacgagttg tactaaggag caggaacatg tggtatttac tttggttcgt cggcattttg
    18541 ttgatgtgtt cgctctccac ccttgtgttg gtatggctgg acccgcgtct gaaaagttaa
    18601 cgaacgtagg cctgatgcgg cgcattagca tcgcatcagg caatcaataa tgttagatat
    18661 gaaaagcgga aacatatcga tgaaagcgat cttaatccca tttttatctc ttctgattcc
    18721 gttaaccccg caatctgcat tcgctcagag tgagccggag ctgaagctgg aaagtatggt
    18781 gattgtcagt cgtcatggtg tgcgtgctcc aaccaaggcc acgcaactga tgcaggatgt
    18841 caccccagac gcatggccaa cctggccggt aaaactgggt tggctgacac cgcgcggtgg
    18901 tgagctaatc gcctatctcg gacattacca acgccagcgt ctggtagccg acggattgct
    18961 ggcgaaaaag ggctgcccgc agtctgctca ggtcgcgatt attgctgatg tcgacgagcg
    19021 tacccgtaaa acaggcgaag ccttcgccgc cgggctggca cctgactgtg caataaccgt
    19081 acatacccag gcagatacgt ccagtcccga tccgttattt aatcctctaa aaactggtgt
    19141 ttgccaactg gataacgcga acgtgactga cgcgatcctc tgcagggcag gagggtcaat
    19201 tgctgacttt accgggcatc ggcaaacggc gtttcgcgaa ctggaacggg tgcttaattt
    19261 cccgcaatca aacttgtgcc ttaaccgtga gaaacaggac gaaagctgtt cattaacgca
    19321 ggcattacca tcggaactca aggtgagcgc cgacaatgtc tcattaaccg gtgcggtaag
    19381 cctcgcatca atgctgacgg agatatttct cctgcaacag gcacaaggaa tgccggagcc
    19441 ggggtgggga aggatcaccg attcacacca gtggaacacc ttgctaagtt tgcataacgc
    19501 gcaattttat ttactacaac gcacgccgga ggttgcccgc agtcgcgcca ccccgttatt
    19561 ggatttgatc atggcagcgt tgacgcccca tccaccgcaa aaacaggcgt atggtgtgac
    19621 attacccact tcagtgctgt ttattgccgg acacgatact aatctggcaa atctcggcgg
    19681 cgcactggag ctcaactgga cgcttccagg tcagccggat aacacgccgc caggtggtga
    19741 actggtgttt gaacgctggc gtcggctaag cgataacagc cagtggattc aggtttcgct
    19801 ggtcttccag actttacagc agatgcgtga taaaacgccg ctgtcattaa atacgccgcc
    19861 cggagaggtg aaactgaccc tggcaggatg tgaagagcga aatgcgcagg gcatgtgttc
    19921 gttggcaggt tttacgcaaa tcgtgaatga agcacgcata ccggcgtgca gtttgtaatg
    19981 cataaaaaag agcattcagt tacctgaatg ctctgaggct gatgacaaac gaagaactgt
    20041 ctaatgcgta gaccggaaaa ggcgttcacg ccgcatccgg tcactttcag ttttactctt
    20101 tctcggagta actataaccg taatagttat agccgtaact gtaagcggtg ctggcgcgtt
    20161 taatcacacc attgaggatt gcgcctttaa tattaacgcc agcctgttcc agacgctgca
    20221 ttgacaagct cacctctttg gcggtgttca agccaaaacg cgcaaccagc aggctggtgc
    20281 cgacagagcg tcccacgacc gcagcattgc tcaccgccag catcggcggc gtatcgacaa
    20341 tcaccagatc gtaatggtcg ttcgcccatt ccagtaattg acgcatccga tcgcgcatca
    20401 gcagttcaga cgggttaggt ggcacctgac cgcgagtaat cacatcaaag cctcctttgc
    20461 caaaatgctg gatcactttg ttgagctcat ctttacctgc cagatattcc gacaagccat
    20521 gttcattact cacggtaaac aggttatgcg aataaccacg gcgtaagtcg gcatcaataa
    20581 ataacacttt ttgatcggac tgggcgatca ccgctgccag agttgaactg acaaacgttt
    20641 taccactgtc tggcgtcgca ccggtgatca tcagaatgtt attctcagtc tccatcatag
    20701 cgaaatgcag actggttcgt agcgcacgta cggcttccac agcagaatcc gccgggttat
    20761 ccaccgccag gaaggggata tttttagtac gatggcgctg ctgattagaa aataaatttt
    20821 tcttacgcag acgggtgcgt ttatccagcc actcggacat tgggatagtg gaataaacgc
    20881 tgatgccgtg ctcttccagt tgttccgggg cttctacacc acgacgcaac atcgcacgcg
    20941 ccagcacggc actcaccgaa ataaacaggc caagaataaa accaagcacc acattcaacg
    21001 cttttttcgg tttcactggc tgcggctgag tgactgccgg gtcgataatc cgcacgttac
    21061 caatggcact ggatttcgaa atactcaact cctgctggcg gttaagtaat tgcagatata
    21121 ccgcacggcc cgcttctacg tcacgactta aacgcaacac ttcctgttgg gtggaaggca
    21181 ttgccgatac ccgcttattc aggcgtttgc gttcttgctc cagcgtctgg cgtttttcca
    21241 gcagcgcacg ataagttggg tgatctttct tatacagctg ggagatctct gcctcgcgga
    21301 aggtcagctc attgagttga ttatcaacgt tcacaatctg ctcaagaacg gctttggctt
    21361 ccaggttaag gtcaaccgaa tcgcgctgct ggcgataaac gttgagtttt tcttccgcct
    21421 ggtctagctc gctgcgcact tcaggtaact ggcgctgtaa gaattcaagg ctttgtgaat
    21481 cctgcgccgc ctggcgagcg atattctgtt gcaaatagtt gttagcgatg ctgttcagaa
    21541 tacgagtaat caactgggga tcatcaccag tcatggtaag ttccagcatc ccgctttctt
    21601 tactgcgttc gctaacggta aaggtttcct gcaatgcgtt aatcgcttcc agttcggtac
    21661 gctggctcag gacaaactgt gttcctggtt tggccttaat gtccgcgata gtcagcgcaa
    21721 cgccatcttt ttccagacgc tggccgacca taccattgac ggtgaactct tcaccttcca
    21781 gtgtatagtg gccgttttcc ccaaccgtga gtgtcagttg ctgatcctga ccattcagtt
    21841 gtggaatatg catccagctg atcgccagct cacctagttt ttctttggtt aatctcgccc
    21901 agccgcgacc cacaatcgga aaatacttct gctcaactat gtcgcgcaga ttcagttcag
    21961 caatggtttt accgagaatc atgcgcgatt gcagcagttg gatctccggt gcagactcgg
    22021 gcgatgagtt agggatcata tcgctcaggc cgctgagaat ggcgttgccc tgtttttgct
    22081 caacctggac cagagtatct gcctgataaa ttggtgtgct taacagcgag taagcgacag
    22141 cgatcagcgt gaataacgcg gtcacgctga taataaactt acggtgatcc cataactcgc
    22201 cgaccagacg aagcagatcg atctcatttt cctgagtgct gcctggtggc gtattcatat
    22261 ttttagttgt catcgtattt tataaactca aattaccggc tgaggcgctt cgcccattcc
    22321 tgactggcgc gctccaacat accgtagaca tgttcaaatg cgtcctgact tttacgatag
    22381 ggatccggga tctctttctg ttccagccat tgcccaaaca gcattgtctt cccgcggacc
    22441 tcgggagcaa ttgcggtaac ctgagcaata tgttccgact ccattgccag aatcagatcg
    22501 taatttcgtg ccatctcagc agtaagctta cgtccggcat gtccttcaag agatacgcca
    22561 tgattagcgg caacatctgc cgctgtcgca tctgcagggt gttttaccag accatgaaca
    22621 cctgccgatt tgacttttac gcccggcaga cgtttacgca gcaagcgctc gccaatgggc
    22681 gaacggcaaa tgttgccggt acaaaccact aggattgagt taaattttag ttgggccatg
    22741 tttgaatcca ccgtgaagtc tctgtcaggt catgcacccc ggaaatcgtc ggtaccagca
    22801 gagaaatcac acggttccaa cgagccagcg gcgcggtagt gacataaaca atgtcgtaag
    22861 gttcaagctg gaattccgtt ccgagtatca ttgccgatgc gtccttcgca ttcagctgat
    22921 aaatattggc gattttgcca ttctgcttgt tttgcgttgc acgaataaca aaaatccccg
    22981 tcgcatcagc gacatcctga ttcataccct cagcgttacc cagcgcttct gccagggtca
    23041 taccgctgcg gtccattttc agcgtgctct gtttacccac ttcacccatc acgaagactt
    23101 tcagatcatc gttgcgcgga ataaataaaa tatcgcccgg gtgcagcagt ttgttctggc
    23161 gcaagtcgcc acgctgcatc agcgcataga ggttcacttt ggtctttacg ccattctggg
    23221 tgagcaccac gttgcgccag tctgcatctg cggtcagacc gcccgcggcg ttaatcgcat
    23281 ccatgatggt cagaggaata ttggtaatcg gctgctgacc cgatttgctg acttcgccgg
    23341 tgacgtacgc tttttgcgat cggaacgccg cgacgctgac atcgacttgc gggctttcaa
    23401 tgactgaatc gagacgcgct gtaatctcgt tgcgtacctg ggtaagtgtt ttcccggcca
    23461 ctttcaggcg accaatatag gggtagaaaa tcgcaccatc ggcgttaacc cagttgccgg
    23521 tatcactggc gctacggtat tgccctgcgg gggtggttag ttccgggtga tcccagaccg
    23581 taaccatcaa cacatcacca atcccgatac gatattcata gtttgcgatt tgctgctcca
    23641 gctccgggtt agcctgcgac ataatcggtt tggcgcgcag ctgatcaatc aatcctggcg
    23701 tgacgggata gatattgacc attttgtcca ggtcatactg attgtcaggt agttcaatga
    23761 catccttgtt actggtcgat aaattctgac caggaacaag ggtacatgct gtcaaagaca
    23821 gaacagccaa tgtcagtacc gaaaacttaa aaatgttctt tttcattggt ttatatttat
    23881 cagtcaatgt aatgttatga attacgtcag gttattgtcc aaccggtact tctcgctctg
    23941 ccgtcattgg atcgagctgg tatttgcgtg acagcatttg tccaccatca cgcgtcaatg
    24001 gtgtccacga aaccaccgcg cggttgcggt taggtccaat ggtcatcaag tcgaacggaa
    24061 tcgagatata aaaaccttta ctaaagccgc cttcgccgta gtcatcttta gatacgttac
    24121 tgattgccgc ccatacccct accgccacgc cgctgtcaaa gcgtttggcg acgtcgatcg
    24181 ttgcgccttt atcttttgcc agatattgcc caacgctaag tttcatcagt acgccattga
    24241 gcgtcggcgg gttccagtaa gccgtcacga aacctgtggg cgtggaataa tcggtgaagc
    24301 gcatcatgtt gtcccagtca cgctgcttca cgtagttaac gtccacaccc agcgcccagc
    24361 tggcatctag cgggcgataa agaagctcgg aaccgacacc ggcatacatc gtttccagat
    24421 aaccgccata cacctggcca tagaaaccat tgcctaagtc ggcaaagtaa ttcgcttgca
    24481 agttgttgag ataaacgtca ttgcgaacgt aatcacggat atgcgtgcgc acgcggggca
    24541 gggtagagtc cgcgggtaac agtgaagact taaacttgtc gtagttgttg taaatattgg
    24601 tgaaaatacc gtcatcaagc agcaggtggt cggtaaacca gtagcgggcg ctggacatca
    24661 gccccagctg gaacatatag aaatcttccg ggccgcccag cgactgtgaa agtgttgggt
    24721 tgaaagagta gctaaagcga tcttcacgaa tacggtagcc ccgaccaaag gcagaaagat
    24781 cttctgcttc aacacgttgt tgttgcagtt gctctgattt accaggcgct gtgccggcca
    24841 gctgtttgcg caggctggct acgtcggttt cggtagtcac catcgccata tgctcgcgct
    24901 tttgcgtcac gctaatcttc tcaacgcctt gcggcaggtt attcaccaga atccggttgg
    24961 cacgatcgac cgcttcgcga gagtcacggt atttgtattg ctgaccagac atatacagcg
    25021 tcttatcgcg tagctgaatt tctggcgcgt caaagcccgc gttatacttc agggcggtaa
    25081 gttggtttgc taccgtggtg tactgcaaac cttcagactc tggcgcaggc tgccaggccg
    25141 gttttggcgt atcgcgtagg gcagggcgca gatcgttgaa attggtccgt aaagtgaaac
    25201 caaacatcaa cgtgtttccg cgttcataac tgaggttgag gtctgcccag ctggcagcgc
    25261 gataaactgc accgacgttg aaatggcttg cctgaggcag tttgccagcg aaatcattct
    25321 ggtaattgtt gccgtcgtat tcgagtttca gacgcagggg attccacggc gtttgatact
    25381 caatgccgcc aaagatggaa gccgggccac gaaagatatc gctaaagctg atatcgcccg
    25441 catcgtgaga ctctgcgcga tgacaatatt tatcgctcac ccggcaaaac gggttggtaa
    25501 tattgcccgc attaccggcg tagccccatg ccatcccgag ggtgaaatca aatggccccg
    25561 cttgcttgct ggccaccaga tattcaccgt caaacaaacc cgtaccagca atatcacgtt
    25621 taccaaacgc cacttgcggt agccagtaac cttcttccca caggcgaagt ttaaaatcga
    25681 atgatttgtc tttgtatggc tgatcgccgc tgaaatcttc ccactggcta tatttgcgtg
    25741 tgcgcacatc cgtataacga atggtgcctt ccagccatgg gaacagcgcc acggaggtgg
    25801 agtagaaccg gtattgatcg ttatcccggt agttgacgct gaattcacct tccggtgcga
    25861 tgcgcgcatt tggcatctgc aacaatcctg tgccgccaaa gtctgattgc gacgaaccca
    25921 gcggatccgg gtaggttaat acttcagcat gacaggcact ggagacggca atggccaggc
    25981 agcttaaaag ataagaattc ttcttcatca gtcaggaaca cgttgcgtta aaacagacac
    26041 aatctggtca tttaaatcag catatttttc gggcagtaca tgcgcagaaa agcccaacca
    26101 aaactggctg ccaggcggtg gttccacatg tcttttattc cacagggcaa caggggcgac
    26161 gacagtctca ccctcaggcg taatcaccat aacgttattc ttgtctgcgc ctgcaaggcg
    26221 ggggtgatct tgcagataat cggtaacgct acggcctgcc tgccagggca attgcccagc
    26281 cccagaaacc gcacccagca gagtaatagt caccggacgc tgtacggtat agagcgtata
    26341 gtccccgacc aacgggggat tgctgttctc atccactcgt acaaaatcag ggtccagttt
    26401 gaccggtaag cgaccagtga tattgagatt gagtaactgc tggcgcacgg atttaatcgt
    26461 tgccgccaca tcgtcgtcag cttctgcttc ccaggaagca agctgtgcca tgacatgttg
    26521 gtagtcttta agcgctttag ctttcgccgc agagtcggtc agcaaggcac caggccacca
    26581 gaggcgatcg cgcagttgcg gttgtgtcac caactggaca acattttcca cggggccaac
    26641 ggaaagtgtc tgttgttcgc taggcagata aatagtcacc gttccttgcg caaaggcatg
    26701 gggtgtcatt acgtaaagta cgctggcaat gaaatacgac tgtaatttat tcatggtgcg
    26761 ggtttaagaa acgtcatttc tacgggaata acccctgcgc ctaacatttg ccgactttga
    26821 cgaacctggc cagtagccga atcgacccag aatgtgtttt gccactgttt ttctggcagc
    26881 gtggaagtca cctcttcctg ccagacatta cacagcacat tgtgacccgc aatattcagc
    26941 gtctctaatc cagcaaagga aaaagtagaa cttagggtcg cagagcgaaa atggttgtct
    27001 tcactccaga gaatatcgcg cgtccagcgg ctgccatctt ttattgccag cgcgttacgc
    27061 agaggggccc gcccgggatt tgtcacttcc agcagattat tattaagttt gacggtcttt
    27121 aaaagacgtc cattgtgggt aaccagcatg gcgttatcct gggataacca tttgctttgt
    27181 tcttgttcta tatatcccag taccacaaag attcgttgcc cttcattaag gcgtaaatac
    27241 atcgtggaat aaggcaacgc ctggatctgc tgagccgcta cagtgatatc ctgattatcg
    27301 aaaaggctgg cgcgaaatgt atcgaccata ctttgctggc tgtgcgtaca tcccgcaaga
    27361 aatagtgcga aaatcgataa aataagaggg cgcaccttta atccttgaaa ggttaattaa
    27421 aaaaatgcaa atctctaaag cccggtacga atatcgcaac cgggtattat ttaacttgaa
    27481 tttcgacgcg tttaagcaga attagcgcgt agtggacgtg gtggttgagg tgttatgaga
    27541 accgccatcg ccactgtcat ttgctgcaac tactgccaac gtcgccacac caacagccgt
    27601 tgccacaccg acaactgtcg ccgtgccgac agcgccagca gccacgccac tagtggtagc
    27661 cgcagtcgtg gttgcacctg tagtggtcgt tgtggtgcca gtagtacccg tcgttgcctc
    27721 ggtcccgttt gctgcttcag gggcagcaaa cgcagctggc gtcgtcaaca tgaaggccat
    27781 aaggattgca gaaagcttgt gtttcataac ttttccttta ttcatcgcat ggacaatacg
    27841 tacgcttgag atcaggattg tctactttga gtataacatc cttgtgaaat ctccctttcc
    27901 tcataccgct agcactatat actgcgggtg ggaaaggcgc gcagagggaa atataagatt
    27961 gttaacgatt tccctctgga aatacgactt gtgagcataa gataaatctt agaaaaatac
    28021 gaattaaaga taagaaaatt gttacgaaag ctattaatta ttgttggtaa tatagtttca
    28081 agtggtacta taactggctg ttaaatggca tttataacta ttaggtgcag agatattcgc
    28141 ttaaatggat tagtttctta gtggaaaagt aactgatatt ataatgtatg gtgttgacta
    28201 ttgaactatt ccaataaaac cagtcagctt taaacaagca gcgtcatatt aagagagata
    28261 aacatttgcc gctgttggtc ctcgcaggcc atttacgcgg caaaattcca cgcgtaatcc
    28321 tggtataagc acttctgcgt cgcggggagt gaatgcggaa atatggacct ggacttcttt
    28381 acgaccgtcg gaggggataa tgaatccttt gccgcttttg cgatcaaagg ttttgacaat
    28441 tcctgtcatt ttacgggaca aacaaattcc ttactgaaaa tactgcgctg cactatacgg
    28501 ggttaataaa ataaagccag cgatatttaa gaccgccgga cggctaaaat aaaatttgct
    28561 taatctcaat tatcatgcct taatagctgc gtcggtttga aagacagaca gcatacaaag
    28621 tagtttacta aagcagttct ca
//
LOCUS       DAJVPP010000051        28131 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVPP010000051 DAJVPP010000000
VERSION     DAJVPP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.051"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            556..1326
                     /locus_tag="OQM34_002178"
     CDS             556..1326
                     /locus_tag="OQM34_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS1452616.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1317..1790
                     /locus_tag="OQM34_002179"
     CDS             1317..1790
                     /locus_tag="OQM34_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1452617.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1897..2436
                     /gene="dnaT"
                     /locus_tag="OQM34_002180"
     CDS             1897..2436
                     /gene="dnaT"
                     /locus_tag="OQM34_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1452618.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2439..3176
                     /gene="dnaC"
                     /locus_tag="OQM34_002181"
     CDS             2439..3176
                     /gene="dnaC"
                     /locus_tag="OQM34_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1452619.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3225..3719
                     /locus_tag="OQM34_002182"
     CDS             3225..3719
                     /locus_tag="OQM34_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1452620.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3973..6264
                     /gene="opgB"
                     /locus_tag="OQM34_002183"
     CDS             3973..6264
                     /gene="opgB"
                     /locus_tag="OQM34_002183"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1452621.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFNGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6402..7424)
                     /gene="lgoD"
                     /locus_tag="OQM34_002184"
                     /pseudo
     CDS             complement(6402..7424)
                     /gene="lgoD"
                     /locus_tag="OQM34_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7563..8477
                     /locus_tag="OQM34_002185"
     CDS             7563..8477
                     /locus_tag="OQM34_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1452622.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8692..9758
                     /locus_tag="OQM34_002186"
                     /pseudo
     CDS             8692..9758
                     /locus_tag="OQM34_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9807..11471)
                     /gene="tsr"
                     /locus_tag="OQM34_002187"
     CDS             complement(9807..11471)
                     /gene="tsr"
                     /locus_tag="OQM34_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCS1452623.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11590..12036)
                     /gene="hpaR"
                     /locus_tag="OQM34_002188"
     CDS             complement(11590..12036)
                     /gene="hpaR"
                     /locus_tag="OQM34_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1452624.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12308..13597
                     /locus_tag="OQM34_002189"
     CDS             12308..13597
                     /locus_tag="OQM34_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1452625.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAIWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13594..15060
                     /gene="hpaE"
                     /locus_tag="OQM34_002190"
     CDS             13594..15060
                     /gene="hpaE"
                     /locus_tag="OQM34_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1452626.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQDYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMHVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15062..15913
                     /gene="hpaD"
                     /locus_tag="OQM34_002191"
     CDS             15062..15913
                     /gene="hpaD"
                     /locus_tag="OQM34_002191"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1452627.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15923..16303
                     /locus_tag="OQM34_002192"
     CDS             15923..16303
                     /locus_tag="OQM34_002192"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1452628.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16371..17174
                     /gene="hpaH"
                     /locus_tag="OQM34_002193"
     CDS             16371..17174
                     /gene="hpaH"
                     /locus_tag="OQM34_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1452629.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17185..17988
                     /gene="hpaI"
                     /locus_tag="OQM34_002194"
     CDS             17185..17988
                     /gene="hpaI"
                     /locus_tag="OQM34_002194"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS1452630.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18010..19365
                     /gene="hpaX"
                     /locus_tag="OQM34_002195"
     CDS             18010..19365
                     /gene="hpaX"
                     /locus_tag="OQM34_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1452631.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19375..20265
                     /gene="hpaA"
                     /locus_tag="OQM34_002196"
     CDS             19375..20265
                     /gene="hpaA"
                     /locus_tag="OQM34_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCS1452632.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPSNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20292..21229
                     /locus_tag="OQM34_002197"
                     /pseudo
     CDS             20292..21229
                     /locus_tag="OQM34_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21456..23014
                     /gene="hpaB"
                     /locus_tag="OQM34_002198"
                     /pseudo
     CDS             21456..23014
                     /gene="hpaB"
                     /locus_tag="OQM34_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23032..23544
                     /locus_tag="OQM34_002199"
     CDS             23032..23544
                     /locus_tag="OQM34_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1452633.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23937..26087
                     /gene="btsT"
                     /locus_tag="OQM34_002200"
     CDS             23937..26087
                     /gene="btsT"
                     /locus_tag="OQM34_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1452634.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26137..26340
                     /locus_tag="OQM34_002201"
     CDS             26137..26340
                     /locus_tag="OQM34_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1452635.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26351..27307
                     /gene="yjiA"
                     /locus_tag="OQM34_002202"
     CDS             26351..27307
                     /gene="yjiA"
                     /locus_tag="OQM34_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1452636.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27376..>28131)
                     /locus_tag="OQM34_002203"
     CDS             complement(27376..>28131)
                     /locus_tag="OQM34_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCS1452637.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6901 a         7356 c         7035 g         6839 t
ORIGIN      
        1 tttccagcaa attaatagat taagcgaata aattatcctt agcattcact ttcatatcag
       61 catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat caaatgataa
      121 cctggtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag aaatacttaa
      181 tgaaaaacag aaatctcatt catttaattt ataattaaat gatatctctt cccgcagaat
      241 gcgggaatca cacaatcaat atttattcaa taaaatctga tgattttaac ttgttaacac
      301 taaaaaacgc gcccagctat ctattatcct gacccgtaaa ggctaaacaa taaattcatt
      361 atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac
      421 cgagcttgtc ccaataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa
      481 caaccgtgct tcatgataca aatggattct cattatctga acaatacgca gcacgtctat
      541 gacaaagggc gagttatgca aactgagcaa cagcgagccg taacgcggct ttgtatccag
      601 tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca
      661 cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc gaacgccata
      721 gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc
      781 ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat
      841 ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcaaccatt acgttaccca
      901 agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac
      961 ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat gtatatccgc
     1021 cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac cgctttcgcc
     1081 gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt
     1141 gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa tgccgtcgcc
     1201 gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg
     1261 acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct acgcggatgg
     1321 gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc attcctgcgg
     1381 tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg
     1441 gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt
     1501 caacctttat ggcttctatg ctggtcggta ccattggtat tcaatggtcg cgctggtatc
     1561 tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg
     1621 cttataccgc aatgatttcg tcggtaaaaa tcagccagtt aggttacagc gaaccgttga
     1681 tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg
     1741 gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg
     1801 cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
     1861 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
     1921 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
     1981 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
     2041 cgcctggctg aactgctggc gctggaagaa aagctagcgc gtccgggaag cgatgtcgct
     2101 ctggacgacc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
     2161 ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg
     2221 ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
     2281 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
     2341 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa
     2401 cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
     2461 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
     2521 agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa
     2581 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga
     2641 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
     2701 cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
     2761 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
     2821 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
     2881 atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
     2941 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
     3001 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
     3061 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttat
     3121 gggtgatctt caactgggat agctaccgta gccgggtaac gggtaaagag tattaatccc
     3181 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactatta
     3241 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
     3301 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
     3361 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccaag
     3421 ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa
     3481 agctggcttc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
     3541 tgaacagtga agagcaaaaa gaagacacca actatctgga cggtattcag ggtttgctga
     3601 aaacaaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa
     3661 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
     3721 ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc
     3781 ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt
     3841 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
     3901 gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
     3961 gaggatcgcc cattgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
     4021 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacgttaac ggtgctgggg
     4081 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
     4141 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
     4201 atcctgccgg gtatcggcat tgtgctgggg ctgacggcgg tgttcggtgc gctgggctgg
     4261 atcctgcgcc gtcgtcgtca tcatccgcac cattttggtt acagcctgct ggcgctctta
     4321 ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga actggtgaaa
     4381 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
     4441 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
     4501 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
     4561 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
     4621 gcttctcagt gcggcatacc gctgttcgcc ccctttgaag gcaacgcctc cgcctctgtc
     4681 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
     4741 aactatttcg tgcagggcgc aaatctgcgt tttgccggta aagatgtgtt cctgaaatcg
     4801 cacggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtagc cgacccgcac
     4861 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
     4921 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
     4981 cacccggatg gttttatctc gcgcacctgt aaccgcaaaa aatatgattt taacggtaag
     5041 ccgaatcagt cattcagcgc ggtaagttgc agccaggaga atatcgcgac gtttatcaac
     5101 aaaatcaaag cgtcaccgtg gtttaaagat actgtcatcg tcgtctcttc tgaccattta
     5161 gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa cctgtttttt
     5221 gtcattcgtg gcgacaagcc gcagcaaaag acgctggcag tgaagcgtaa cacgatggat
     5281 aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc
     5341 agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
     5401 tggaagccag atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
     5461 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
     5521 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
     5581 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgacag ttgctacaag
     5641 atggcacaac tctgggctcc ggaactggca ctttccaccg actggtgtgt ctcgcagggg
     5701 cagcttggcg gtcagcaaat tgttcagcat gttgacaaaa caacatggaa gagcaagacg
     5761 gcatttaaag atacggtcat cgacatggcg cgttacaaag gcaatgtcga tacgctgaag
     5821 attgttgata acgatattcg ctacaaagcc gacagtttca tctttaacgt cgccggtgcg
     5881 ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg ccgctggtcc
     5941 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc gaagaaattt
     6001 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
     6061 gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac cacactgcat
     6121 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
     6181 aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat ggtggaaatt
     6241 aaagtggtag aacgtgaagg gtaattcctg actgatgtgt aggtcgcata gggtgcgacc
     6301 tacacgcctt ctccggctta caacacatta ttaagaaaca aaaaaaacag cgcccaacac
     6361 ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt aattacaccc
     6421 ttaatcaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac
     6481 ggatagcgat gggttaacat catgtcggca gtgattttcc cttccgccat cagtcgaccg
     6541 actttagcga aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta
     6601 tggaattccg gatcggagaa ctgcaactca cctttaaaca gaccgacaaa taccaccgtg
     6661 ccgccgtgac gaatcagatt cacggtatta ttcatcgcat gttgattacc tgtcgcgtcg
     6721 atcactttct gcgccagcga accaccaaac tgcgcccgca gctgagcgtc aaaatcttcg
     6781 gctgacgggt ccagcacggg caactccaga cgcgttgcca catgttcacg gcgcgccgga
     6841 ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga
     6901 ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc
     6961 accgcatgag cgctaatagc gaaaggttca atcaatgcgg ccgcctgcgg gtcaataccg
     7021 tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga
     7081 tgcacgccaa tgactgaaat tttttcacag cagttggtac gcccgctttt acacgccggg
     7141 cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca
     7201 atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac ccgtggataa
     7261 ctaaaaaatg gttgatttcc accctaggca tgaatatcgg taccgcaaat cccgacagac
     7321 ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact
     7381 aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg gctatccatt
     7441 ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa aatctgtttt
     7501 gtgaaaacac acgcataaaa atgttttaaa tcgggtttta atggactcac aaggaggaaa
     7561 atatgagtcg ttcacaaaat ttacgccaca atgtgattaa ccaggtcatt gatgatatgg
     7621 cgcggggtca tatcccttcc ccacttccat cgcaaagtgc gctggcggaa atgtacaaca
     7681 tcagccgcac tacagtgcgt catattctca gccaattacg cgaatgcggc gtcttaacgc
     7741 aggttggaaa cgactatgtt attgcgcgta aacctgacca tgatgacggt ttcgcctgta
     7801 ctaccgcttc aatgagtgaa caaaacaaag tgtttgagca ggcttttttc acgatgataa
     7861 accagcgtca gttacgccct agggaaactt tttctgaact gcaactggct cgggccgcgg
     7921 gtgtcagccc ggtggttgta agagaatatc ttttaaaatt cggccgttat aatcttattc
     7981 aaagcgaaaa gcgcggccag tggagcatga agcaattcga tcaatcctac gccgagcagc
     8041 tgtttgagct acgcgaaatg ctcgaaacac attcacttca gcactttctc aacctgcctg
     8101 atcatgatcc ccgctggctg caggcgaaaa ccatgctaga acgtcatcgg ctactgcgtg
     8161 acaacatcgg caatagcttt cgcatgttct cgcaacttga cagagatttt cactccttac
     8221 tgctttcagc tgccgacaat atcttttttg atcaatcgct tgagatcatc tcagtcatct
     8281 ttcattttca ctatcaatgg gacgaaagcg atcttaaaca gcgcaatatc attgccgtcg
     8341 atgaacatat gaccattctc agcgctctga tttgccgcag tgaccttgat gccacccttg
     8401 cgctccgtaa ccatttgaat tcagctaaac aatcaatgat tcgatccatt aatgagaaca
     8461 cgcgttatgc tcattaagta cggattaaaa acagcaaact attagcgggt gcacggtttt
     8521 atcaaaagat aaataccggg aaattcccgc ttacctatgc tcacaatcag tcgactaaaa
     8581 atgtttttgt gagcggtagt aaagtcctaa aactttaacc tgccgttaat gtcaaccttg
     8641 cgtaatgcac aattacgtaa acaattaaaa ttataaatac aggagtccgg cgtggaaaaa
     8701 gaaaatatca ccctcgatcc gcgttcttca tttactccat cttcgtcggc agatattccc
     8761 gtgccaccag atggattagt tcaacgcagt acccgaatta aacgcattca aaccaccgcc
     8821 atgttgttat tattttttgc ggcggtaatc aattatctcg accgcagttc gctgtcggta
     8881 gcaaatttaa cgattcgtga agaattggga ttaagtgcca ccgaaatcgg cgctttgctc
     8941 tccgtgtttt cactcgctta cggcattgcg caacttcctt gcgcgggatg tttattacca
     9001 ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc aaccgcgagc
     9061 acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc aatgcccgcc
     9121 gagatccgct caattttgcc gaatggcgca gcctgttccg taaccgcaca atgtggggaa
     9181 tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc tggcttcctg
     9241 gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg gcggctatcc
     9301 ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac tggctggtca
     9361 aagggggaat ggctccgatt aaaagccgta agatctgcat tattaccggg atgttctgtt
     9421 ctgccgcctt tacgctgata gtaccacaag cgacaacatc catgatggcg gttctgctga
     9481 ttggcatggc actgttctgt attcactttg ccggaacatc ctgctggggc ttgatccacg
     9541 tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt gccagcttca
     9601 tctgcgcctc tttcgcgccg atcattactg gttttattgt tgatacaacc cactcattcc
     9661 gtctggcact aatcatttgc ggttgcgtca ccgcagcggg agcactggcg tacatcttcc
     9721 tggttcgcca gccgatcaac gacccgcgta aagattaaac tccctatgcc cgataacatt
     9781 ttgcttatcg ggcattttta tggcgattaa aacgtttccc agttctcccc gctatctgcc
     9841 actgccattt tacgcggcgt agctggcgtc acggttttta ccacagccga tgtttcacgc
     9901 tgctgttgct gctgttgctg aatccggaac actgccacag cttcggtcag gcgactggcc
     9961 tgctcttcca gcgcggcggc ggcagcggca gattcttcca ccagcgcggc gttctgttga
    10021 gttacccggt ccatctcagc aaccgctaag ccaacctgat cgataccacg gctctgctca
    10081 tcagaagcag aagctatttc gcccataatg tccgtcacgc gggtcacagc actgacaatc
    10141 tccgccattg tttccccggc gctttcgacc agcgtagagc caacatccac tttccccacc
    10201 gagtcttcaa tcaggctttt aatttcacga accgcctggg cgctacgctg ggccagatta
    10261 cgcacttctc ccgcaaccac cgcaaaacca cgtccttgct cacccgcacg cgctgcttca
    10321 accgccgcgt tcaaagccag aatattggtc tggaaggtaa tgccgtcaat tacgctgata
    10381 atatcggcga ttttctgcga actggtggag atatcgcgca tagtctgcac cacgttatct
    10441 accactttgc cgccgcgttg cgccgtttca gaagcactta atgccagatg gctggcctgg
    10501 cgcgcattct cggcgttctg tttcaccgtt gcggtcagtt gctccatgct ggctgccgtc
    10561 tcttccagcg aagcggcctg ttgctcggtg cgcgaagaga gatcattatt gccggtagcg
    10621 atttcgctgg caccgctata gatggcattg gccccgttgc gcacatcacc gacggtacgc
    10681 atcagctctc cctgcatatg gcgcaaactc tctgccagtt gccccatctc attagagcca
    10741 tccacctcaa tcggtttcac cagatcgccg cctgcaatat gacgaatgct gtcaatcagg
    10801 cgattcattg gcgctaccag cgaggcttta ataccgaacc agacggcgaa gatgaccgcc
    10861 agtacgacga tcatcacgcc caccagaatc cacatcgcct ggctgtagga ggcattgtta
    10921 tcgctgacgg cgatatcata gagccgatcg ttttgctcca tgtaagccac atactgcttc
    10981 tcgaaaccgt cctgatatcc ctgggtcggc tgatcaaaga actcgttgat tttgcctgca
    11041 cctaacagtt ggatcagctc cgccagcgca ttgtgataaa tatcgtaatt acgtttgatc
    11101 tctgccgctg cggcggtgct ctgacgcggg tcacgcggca acgcttcgta atccgcccag
    11161 tttttttccg cctgtttcag cgaaatactg gcactctgca tcagctcagc aacggttgaa
    11221 ccgctaccaa tattattctg atccatcatg tagcggatac ccgcgcggtt gagggtgtta
    11281 cgcgtctgca acaacgcgac ccagctgcca ttcagcgtgg gttgctgctg gcgaatggtt
    11341 tgtaaaacag tgaaattttc tttgtcattc tttaaggcat taaaaaacag accgcctgat
    11401 gtcagttgta aaaggccaaa aacggccaaa accagcagta agctggtcac gatcttgata
    11461 cgttttaaca tggtttctct ttcctgtgga ccgatgcaag attttcggcc tggaaagcaa
    11521 aaactttatg aaaaggtgta acccgccatc catggaaaaa ccaggcgctg gttatcagat
    11581 actaaaaagt tattcattat cgcccgggat gtcttcttgt cgggaattgc ccagagcaat
    11641 aaattcctct aacagatggg ttaactgttg cattttctcg gcagtaaact gagcttcaat
    11701 ctgccgatag gcttcttcaa tctgtgtctg agcgcggtta tacagcgcct gcccctcttt
    11761 ggtcagcgag atatagagct tccgctgatc attgatcggc tttaatcgca acactaaacc
    11821 gtcgcgttcc atccgcgtga ggatcccggt caggcttggg cgcaaaatgc aggcgcgata
    11881 cgccagatcg tgaaaatcca ttgatgggct ttccgccaga atacgcacga tgcgccactg
    11941 ttgctcagtc aaattatgcc gtttaacgat tgggcgaaaa taactcatcg ccgcttctcg
    12001 cgcctggagc aacgcaatgg ttagtgagtc gtgcattatc tttcccctga aaaattcaaa
    12061 atcattaata tagaaacaat taatcatcac gttaccggga ctgagctgcc cccgcaattc
    12121 ttcattttat caaaattgat gatattttat taacttactg atatttaata ttttattatc
    12181 tttaaatgta aatagtttgt taattaaatc acatttatat cacttacttt tgccaaacta
    12241 cgcaatcggg aataaaagta atcattaaca tattaatgat taagatgata gaatccgagg
    12301 agtggtaatg aaaggcacta tcttcgccgt agcgttgaac catcgcagcc aacttgatgc
    12361 ctggcaggaa gcgttccagc aatctcccta caaagccccg cctaaaactg cgatctggtt
    12421 tattaaaccg cgcaatacgg tgattggttg cggtgaaccg attccctttc cacagggtga
    12481 aaaagtactg agcggtgcga ctgttgcgct gattgtgggg aaaacggcga cgaaagtacg
    12541 tgaagaagat gcggcagagt acatcgccgg atatgcgctg gctaacgacg tcagcctgcc
    12601 ggaagagagc ttttaccgtc cggcaatcaa agcaaaatgt cgtgatggat tctgccccat
    12661 tggcgaaacc gtggctctca gcaatgtcga taatctgact atctataccg agatcaacgg
    12721 gcgtcctgcc gatcactgga ataccgccga tttacaacgt aacgccgcac agctgctgag
    12781 cgccctgagc gaatttgcca cgctgaatcc aggcgatgct attctgctcg gcacgccaca
    12841 ggcgcgcgtg gaaatacagc caagcgatcg cgtgcgtgtt ctcgcagaag gtttcccgcc
    12901 gctggaaaat ccggtagtgg acgaacgtga agtgaccacg cgcaagagct tcccaacgct
    12961 gccacacccg cacggtacgc tgtttgccct cggcctgaac tacgccgacc acgccagcga
    13021 actggaattt aagccaccgg aagagccgct ggtgttcctg aaagcgccga ataccctcac
    13081 tggcgacaac cagacctccg tgcgcccgaa caatattgaa tacatgcact acgaagcgga
    13141 gctggtggta gttattggca agcaggcgcg taacgtcagc gaagccgatg ccatggatta
    13201 tgtcgcgggc tacaccgtgt gtaacgacta cgccattcgc gactatctgg aaaactacta
    13261 ccgccctaac ctgcgggtca aaagccgcga cggactgacg ccgatgcttt caaccatcgt
    13321 gccgaaagag gcgatcccgg acccgcataa tctgaccctt cgcaccttcg tcaacggcga
    13381 gttacgccag caaggcacca ccgccgatct gatcttcagc gtgcccttcc tgatcgccta
    13441 cttaagcgaa tttatgaccc tgaatccggg cgacatgatc gccaccggca caccaaaagg
    13501 cttatctgac gtggtgcctg gcgatgaagt agtggtggaa gtagaaggcg tgggccgcct
    13561 ggtgaaccga attgtgagtg aggaaacagc gaaatgaaaa aagtaaatca ttggatcaac
    13621 ggcaaaaatg ttgcaggtaa cgactacttc cagactacca atccggcaac gggtgaagtg
    13681 ctggcggatg tggcctctgg cggtgaagcg gagatcaatc aggcggtagc ggcagcgaaa
    13741 gaggcgttcc cgaaatgggc tgatctgccg atgaaagagc gtgcgcgcct gatgcgccgt
    13801 ctgggcgatc tgatcgacca gaacgtgcct gagatcgccg cgatggaaac cgcggacacg
    13861 ggcctgccga tccatcagac caaaaatgtg ttgatcccac gcgcttctca caactttgaa
    13921 tttttcgcgg aagtctgcca gcagatgaac ggcaagacct atccggttga cgacaagatg
    13981 ctcaactaca cgctggtgca accggtaggc gtttgtgcgc tggcgtcgcc gtggaacgtg
    14041 ccgtttatga ccgccacctg gaaggtcgcg ccgtgtctgg cgctgggcaa taccgcggtg
    14101 ctgaagatgt cggaactctc cccgctgacc gccgaccgcc tgggtgagct ggcgctggaa
    14161 gctggtattc cggcgggcgt gctaaacgtg gtacaggact acggcgcaac cgcaggcgat
    14221 gcgctggtcc gtcatcatga cgtgcgtgcc gtgtcgttca ccggcggtac cgccaccggg
    14281 cgcaacatca tgaaaaacgc cgggctgaaa aaatactcca tggagctggg cggtaaatca
    14341 ccggtgctga tttttgaaga tgccgatatt gagcgtgcgc tggacgccgc cctgttcacc
    14401 atcttctcaa tcaacggcga acgctgcacc gccggttcgc gcatctttat tcagcagagc
    14461 atctatccag aattcgttaa acgctttgcc gaacgcgcca accgtctgcg cgtgggcgat
    14521 ccgaacgatc cgaataccca ggttagggcg cttatcagtc agcaacactg ggaaaaagtc
    14581 tccggctata tccgtctcgg cattgaagaa ggcgcaaccc tgttggcggg cggcccggat
    14641 aaaccgtccg acctgcctgc gcacctgaaa ggcggcaact tcctgcgccc aacggtgctg
    14701 gcggacgtag ataaccgtat gcacgttgcc caggaagaga ttttcgggcc ggtcgcctgc
    14761 ctgctgccgt ttaaagacga agccgaaggc ttacgcctgg caaacgacgt ggagtacggc
    14821 ctcgcgtcgt acatctggac acaggatgtc agcaaagtgt tacgcctggc gcgtggcatt
    14881 gaagcaggca tggtgttcgt caacacccag aacgtgcgcg acctgcgcca gccatttggc
    14941 ggcgtaaaag cctccggcac cgggcgtgaa ggcggtgagt acagcttcga agtgttcgcg
    15001 gaaatgaaga acgtctgcat ttccatgggc gaccatccaa ttccgaaatg gggagtctga
    15061 tatgggtaag ttagcgttag ccgccaagat cacgcacgta ccgtcgatgt atctctctga
    15121 actaccagga aaaaaccacg gttgtcgcca gggtgcgatc gacgggcata aagagatcag
    15181 caagcgttgc cgggaaatgg gcgtcgatac cattatcgtt ttcgatactc actggctggt
    15241 caacagtgct tatcacatca actgtgcaga ccattttgaa ggcgtctaca ccagcaacga
    15301 gctgccgcat tttattcgcg acatgaccta caactacgag ggcaacccgg agttggggca
    15361 gctaattgcc gaggaagcct taaagctcgg cgtgcgggca aaagcgcaca atattcccag
    15421 cctgaaactg gaatacggca cgctggtacc gatgcgctac atgaatgaag acaagcactt
    15481 caaagtggtc tccatttcgg ctttctgcac ggttcacgat tttgccgaca gccgcaagct
    15541 gggcgaagcg attctgaagg cgatcgaaca gtacgacggc accgtggcgg tccttgccag
    15601 cggttcgtta tcgcaccgct ttattgacga tcagcgtgcg gaagaaggga tgaacagcta
    15661 cacccgcgag ttcgaccgcc agatggacga gcgtgtggtg aagctgtggc gcgaaggcca
    15721 gttcaaagag ttttgcaata tgctgccgga gtacgccgac tactgctacg gcgaaggcaa
    15781 tatgcacgac acggtgatgc tgctggggat gctcggctgg gataaatacg acggcaaggt
    15841 ggagtttatt accgagctgt tcccaagctc tggcaccggt caggttaacg ctgttttccc
    15901 gctgcccgcg taaggacgtt ttatgccgca ctttatcgtg gaatgtactg aaaatattcg
    15961 tgaagaagcc cgcttacctg agctgtttgc cagtgttaac actgcactgg cggcaacggg
    16021 gatttttccg cttgggggaa tacgtagccg ggcgcactgg atcgatacct ggcaaatggc
    16081 tgatggcaag cacgattacg cctttgtgca tatgacgcta aaaatcggca gcggacgcag
    16141 tctggaaagc cgtcaggaag tgggcgaaat gctgtttgat ctgattaaaa cgcacttcgc
    16201 ctcactgatg gagagccgtt acctagcgct ttcatttgag attgcagagc tgcacccgac
    16261 gttgaatttc aaacagaaca acgtacacgc gttgtttaaa taaatttgca ccagcttcca
    16321 ggccggatga taaggcgctg ccgcatccgg caatgccaca ggatatcgct atgttcgata
    16381 aacacaccca caccctgatc gcccagcgtc tggatcaggc agaaaaacag cgcgaacaga
    16441 tccgcgcgat ctcgctggat tacccggaga tcaccatcga agacgcctac gccgtgcagc
    16501 gcgaatgggt tcgcctgaaa atcgccgaaa gtcgcacgct gaaaggccac aaaatcggtc
    16561 tgacctcgaa agcgatgcag gcaagctcgc agatcagcga accggattac ggtgcactgc
    16621 tggacgacat gttcttccac gatggcagcg atatcccgac cgatcgcttt atcgtgccgc
    16681 gcattgaagt ggagctggct tttgtgctgg caaaaccgct gcgtgggcca aactgcacgc
    16741 tgttcgacgt ttacaacgcc acggactatg tgatcccggc gctggagctg atcgacgctc
    16801 gctgccacaa catcgatccg gaaacccagc gcccgcgcaa agtattcgac accatttccg
    16861 ataacgccgc caacgccgga gtgatcctcg gaggtcgtcc gatcaaacca gacgaactgg
    16921 atctgcgttg gatctccgcc ctgatgtatc gcaatggcgt gattgaagaa accggcgtcg
    16981 ccgctggtgt gctgaatcat ccggcaaacg gcgtggcctg gctggcgaac aaactcgcac
    17041 cgtatgacgt acaactggaa gccgggcaaa tcattctcgg tggttcgttc acccgcccgg
    17101 ttccggcgcg taagggcgac accttccacg tcgattacgg caacatgggc tccattagct
    17161 gccgctttgt ttaaggagag aacgatggaa aacagtttta aagcggcgct gaaagcaggc
    17221 cgtccgcaga ttggattatg gctggggctg agcagcagct acagcgcgga gttactggcc
    17281 ggaccaggat tcgactggtt attgatcgac ggtgagcacg cgccgaataa cgtgcaaacc
    17341 gtgctcaccc aactacaggc gattgcgccc taccccagcc agccagtggt acgcccatcg
    17401 tggaacgatc cggtgcaaat caaacaactg ctggacgtag gcacacaaac cttactggtg
    17461 ccgatggtac aaaacgccga cgaagcccgt aaagcggtac gcgccacccg ttatcccccc
    17521 gccggtattc gcggtgtggg cagtgcgctg gctcgcgcct cgcgctggaa tcgcattcct
    17581 gattacctgc aaaaagccaa cgatcaaatg tgcgtgctgg tgcagatcga aacgcgtgag
    17641 gcaatgaaga acttaccgca gattctggac gtggaagggg tcgacggcgt gtttatcggc
    17701 ccggcggatc tgagcgccga tatgggttat gccggtaatc cgcagcaccc ggaagtacag
    17761 gccgccattg agcaggcgat cgtgcagatc cgcgaagcgg gcaaagcgcc ggggatcctg
    17821 atcgccaatg agcaactggc aaaacgctat ctggaactgg gcgcgctgtt tgtcgccgtc
    17881 ggcgttgaca ccaccctgct cgcccgcgcc gccgaagcgc tggcagcacg gtttggtaat
    17941 agttcttcta tttcgatggc gaagcaaaac aacaactctg tctactaaac gtacgatgtg
    18001 gagcgcatca tgacgacctc taccctgcaa tataaagata ataaaactgt tgaaattgaa
    18061 catcgtgtca ttaataaatt atttcgtcgc ctaatcattt ttctttttat tctgtttgtt
    18121 ttttcgtttc ttgatcgcat caatatagga tttgccggac tgacaatggg caaggatctt
    18181 ggcctgacgt ccacaatgtt tggcctcgcc gcaacactct tttatgtcac gtacgtcatg
    18241 tgcgggatcc ccagtaatat gatgttagcg aaaattggcg ctcgtcgatg gatcgctggg
    18301 atcatggtgg tctggggaat tgcttccacc tgcacaatgt ttgcaacaag ccccgaaaca
    18361 ctctatctac tgcgtatgct ggttggtatt gctgaggcgg gttttttacc tggtattttg
    18421 gtttatctga cctggtggtt cccggcatat catcgggcca gagccaatgc cttatttatg
    18481 atagcgatgc cagtcactat gatgctggga ttgatccttt caggctacat tctggccttg
    18541 gatggactgt ggaatctaaa aggctggcaa tggctctttt tgctggaagg tctgccatcg
    18601 gtcattcttg gtgtcgtcac ctggttttat ctcaacgaca caccagacca ggcgacctgg
    18661 cttgatgaaa acgaaaaaca ggctttaaaa acgatgattc tccgtgaaca agagagtgca
    18721 tttgcccatg catctgttcc acgctcaacc ttacgtgaag tcctgacccc agccatcatg
    18781 ctatatacac ttgcttattt ttgcctgact aatactctga gtgcgattaa tatctggacg
    18841 ccgcaaatcc tacagagttt caatactggc agtagcaaca tcatgattgg tttgctggcg
    18901 gccatccccc aattctgcac aatttttggc atgatctggt ggagtcgtcg ttccgaccgt
    18961 cttaaagaac gcaaaaagca tacgattctt ccctacctgt ttgccgctgc cggatggtta
    19021 ctcgcttctg cgacagatca cagcctcatt caactgagca gcatcattat ggcctcaatg
    19081 ggatcattta ccgcgatgac tatcttttgg acaacaccgg atcaagttat aagcttgcaa
    19141 tcgcgtgccg ttgcgatggc tgtcatcaat gctgtcggca atgtaggttc agcagtcagt
    19201 ccattactta tcggtatttt gaaagatgca accggcagtt ttagctcagg tttgtggttt
    19261 gtagcaggat tgctggtact gggcgcgtta gtcatgaccc gaatccctat ggggagtcgg
    19321 gaacactctc tttcatctga acttatcgca ccaaaaccgc aataaggagt catgatgtcc
    19381 agtgaagcta tctttgaaca tggttatgaa aatattgatt tggtccggga atatgatgcc
    19441 cgctacgctg gtgaagatgt gcattatgaa acatttgccc ggttagcgaa tttctttgga
    19501 cgcgatatgc gccctcactg gcacgataga tattttcaac tccattatct ggtcactgga
    19561 cgcattactt tacaactgga tgagcacttc tattctgtgc gcgcaccgct ttttgtacta
    19621 acaccaccat cggtgccaca tactttcttt actgatcctg atagcgatgg gcatgttctg
    19681 accgtacgtc aggagttaat ttggcccctg gtagaaaagc tctggcctgg tagtggagat
    19741 gccgcaatgc tacaaggtat ctgtctgtca ctggagggaa tggaacatac acaggcggca
    19801 ttaaatcatt attggccgat aatatccctg gagtttcaac aaaataagca aggacgggaa
    19861 attctattgc aatcactggc gcaaagtatt tttacaatcc tactgcgtga agcaccatca
    19921 aatgatattt caacatgcag cgtgcgcggc gagatgtgtt tattccagaa gtttaatcgg
    19981 ctgattgacg aaaattatcg ccaacattta atggtccctg aatatgcaac aatgctcggt
    20041 atgagtgaat cacggcttac cgagctgtgc cgacgttttg ccaatcaatc gccgaagaga
    20101 ttgattttcg aacgagtgag ccgggaagcc agacggcttc tgctctatag cgagcaaagc
    20161 atcaaccaga tagcccttga tcttggctac aaagatcccg cctatttcgc ccgttttttc
    20221 aaccgaatga tgggttgctc accaacacaa ttccgtggtc gctgaccatt tatcaacctg
    20281 agagactcga catgaaaata tctttacgct cactatttgt ggcgtgcctg attgcgccag
    20341 tggcattcac ctacgcctcc gaaccgcaat aaatgaaaat tgtactactt ggaacgaaag
    20401 gtggcccttc tttattaaaa gctgaccgtt taccgatggc aacggcactt attattggca
    20461 ataaaacctg gttaattgat gctggttacg gggccagctt acaactggta aaaaataata
    20521 ttccactgcg ccagattgat aatattttac tcacccattt acacagtgat catattctgg
    20581 actatccatc agtcttaatg aatgcatggg ctagcggtct gcaggacaaa actattcatg
    20641 tattcggtcc gccaggcacc cgagccatga ccgctgccag ttggcaggtc ttcgatcggg
    20701 acatcacact gcgtatggaa gaagaaggaa aacccgatcc tcgcaaactc gttacagctc
    20761 aagatgttgg tgaaggcgta gtttataaag atgataccgt gaccgtgact gcattaaagg
    20821 tccctcattc tcctttccct gacggtgagg cttttgctta tcgcttcgac acacaaggca
    20881 agcgaattgt cttctcagga gatacctcgt actttccccc actggcaact ttctcagaag
    20941 gcgctgatat acttgtgcat gaggccgtac atgagccctc cgtcgctaag ctggcagaca
    21001 gtattggcaa tggtaaaaca ctggcaaaag ccattgcttc tcaccatacg actattgagg
    21061 atgtaggcaa aattgccaat gaagcaaaag tgaaaaaact catccttagc cacctggtac
    21121 caatcaccgt tccggatgaa acatggaagc aagaagcaca gaaaactatt caggccctat
    21181 cgtcgttggt catgaaaata tgacgattac cattccattc gccccataaa aaattccgtc
    21241 cccgttagcc cttgttagcg gggaatatta tgatttgcct tactgtaagt tgatcacgat
    21301 cgcagtttac tatttaactc caccttgctg tcgaaaagta ccaacgttcg cctgaaacgt
    21361 ctcttcccga caaccgcgtt caacgcttca attaaataac aaaaacaaca cacaaaatta
    21421 acaatcgtat tccgattaat actgtagagg tcgacatgaa accagaagat ttccgcgcca
    21481 gtacccaacg tcctttcacc ggggaagagt atctgaaaag cctgcaggat ggtcgcgaga
    21541 tctatatcta tggcgagcga gtgaaagacg tcaccactca tccggcattt cgtaatgcgg
    21601 cagcgtctgt tgcccaactg tacgacgcgc tgcacaaacc ggagatgcag gactctctgt
    21661 gttggaacac cgacaccggc agcggcggct atacccataa attcttccgc gtggcgaaaa
    21721 gtgccgacga cctgcgccag caacgcgacg ccatcgctga atggtcacgc ctgagctatg
    21781 gctggatggg ccgtacccca gactacaaag ccgctttcgg ttgcgcactg ggcgcgaatc
    21841 cgggctttta cggtcagttc gagcagaacg cccgtaactg gtatacccgt attcaggaaa
    21901 ctggcctcta ctttaaccac gcgattgtta acccaccgat cgtcatttgc cgaccgataa
    21961 agtgaaagac gtttacatca agctggaaaa agagactgac gccgggatta tcgtcagcgg
    22021 tgcgaaagtg gttgccacca actcggcgct gactcactac aacatgattg gcttcggctc
    22081 ggcacaagtg atgggcgaaa acccggactt cgcactgatg ttcgttgcgc caatggatgc
    22141 cgatggcgtg aaattaatct cccgcgcctc ttatgagatg gtcgcgggtg ctaccggctc
    22201 gccatacgac tacccgctct ccagccgctt cgatgagaac gatgcgattc tggtgatgga
    22261 taacgtactg atcccatggg aaaacgtgct gatctaccgc gattttgatc gctgccgtcg
    22321 ctggacgatg gaaggcggtt tcgcccgtat gtatccgctg caagcctgtg tgcgcctggc
    22381 agtgaaactc gacttcatta cggcactgct gaaaaaatca ctcgaatgta ccggcaccct
    22441 ggagttccgt ggtgtgcagg ccgatctcgg tgaagtggtg gcgtggcgca acaccttctg
    22501 ggcattgagt gactcgatgt gttcagaagc gacgccgtgg gtcaacgggg cttatttacc
    22561 ggatcatgcc gcactgcaaa cctatcgcgt actggcacca atggcctacg cgaagatcaa
    22621 aaacattata gaacgcaacg ttaccagtgg cctgatctac ctcccttcca gtgcccgtga
    22681 cctgaacaat ccgcagatcg accagtatct ggcgaagtat gtgcgcggtt cgaacggtat
    22741 ggatcacgtc cagcgcatca agatcctcaa actgatgtgg gacgccattg gcagcgaatt
    22801 tggtggtcgt cacgagctgt atgaaatcaa ctactccggt agccaggatg agattcgcct
    22861 gcaatgtctg cgccaggcac aaagctccgg caatatggac aagatgatgg cgatggttga
    22921 tcgctgcctg tcggaatacg accagaacgg ctggactgtg ccgcacctgc acaacaacga
    22981 cgatatcaac atgctggata agctgctgaa ataacgcagc aggaggttaa gatgcaatta
    23041 gatgaacaac gcctgcgctt tcatgacgcg atggccagcc tgtcggcagc ggtaaatatt
    23101 atcaccaccg agggcgacgc cggacaatgc gggattacgg caacggctgt ctgctcggtc
    23161 acggatacac caccgtcgct gatggtgtgc attaacgcca acagtgcgat gaacccggtt
    23221 tttcagggca acggcaagtt gtgcgtcaac gtcctcaacc atgagcagga actgatggcg
    23281 cgccacttcg cgggcatgac aggcatggcg atggaagagc gttttagcct ctcatgctgg
    23341 caaaaaggtc cgctggcgca gccggtgcta aaaggttcgc tggccagtct tgaaggagag
    23401 atccgcgatg tgcaggcgat tggcacacat ctggtgtatc tggtggagat taaaaacatc
    23461 atcctcagtg cagaaggtca cggacttatt tactttaaac gccgtttcca tccggtgatg
    23521 ctggaaatgg aagctgcgat ttaagctcca gccccttcac gcattcgtcg aaggggcatt
    23581 tttattgtga tctcaatcac atatctaccc gatgaataat tcttgcccaa ccgctcaccg
    23641 ccattcctga ccgcttacat ccctaaaata accactcagt tatttacctt actttacgcg
    23701 cacgtatctc tggcaacatc actgcaggat agcggtcaat ttacctcctc aaacgcaacg
    23761 caaacctaga acggcttcgg cctatgatta acaaattact ctatggattt tggtttgcag
    23821 gaaggcagca agtgagtgaa tccccgggag cttacaaaag taagtgtcag aggtgaacga
    23881 acgcagctgc cgcacctgta agccaaaaga cgacgagtaa aatgccaggt tttactatgg
    23941 atacgaaaaa actattcaag cacataccct gggtgattct cggaatcatc ggtgcattct
    24001 gcctcgcggt agttgcatta cgtcgggggg agcacgtcag cgccctgtgg atcgtggtcg
    24061 cctctgtgtc ggtgtatctg gtggcgtatc gctactacag tctgtacatc gcccagaagg
    24121 tgatgaaact cgaccccacg cgcgcgacgc ctgcggttat taacaacgac ggtctgaact
    24181 acgttccgac caaccgttac gtgttgtttg gtcaccactt cgccgctatc gccggtgctg
    24241 gtccgctggt gggaccagtt ctcgccgcgc agatgggcta cctgcctggc acgctgtggc
    24301 tgctggcggg ggtagtgctg gccggtgcgg tacaggactt tatggtgctg tttatctcct
    24361 ctcgccgtaa cggcgcatct ctcggtgaga tgatcaaaga agagatgggg ccagtaccgg
    24421 ggactatcgc gctgtttggc tgtttcttaa tcatgatcat catcctcgcc gttctggcgc
    24481 tgattgtggt taaagccctg gccgaaagtc cgtggggtgt cttcaccgtt tgctcaaccg
    24541 taccgattgc gctgtttatg ggtatctaca tgcgctttat ccgtccgggg cgtgtgggtg
    24601 aagtctctgt tattggtatc gtgctgctgg ttgcctctat ctacttcggt ggcgtgattg
    24661 ctcacgatcc gtactggggt ccggcactga cctttaaaga caccaccatt accttcgcgc
    24721 tgattggcta tgcgtttgtt tccgcactgc tgccggtgtg gctgatcctc gcaccgcgcg
    24781 actatctggc aaccttcctg aaaatcggcg ttatcgtcgg cctggcgctg ggtatcgtgg
    24841 tgctgaaccc ggaactgaaa atgcctgcga tgacccagta cattgacggt actggcccgc
    24901 tgtggaaagg cgctctgttc ccgttcctgt tcatcaccat cgcctgtggt gcggtatctg
    24961 gcttccacgc gctgatctct tccggtacga cgccgaaact gctggctaac gaaaccgacg
    25021 cgcgtttcat tggctacggc gcaatgctga tggagtcctt cgtggcgatt atggcgctgg
    25081 ttgcagcgtc catcatcgaa ccgggtcttt acttcgcgat gaacaccccg cctgctggcc
    25141 ttggcatcac catgcctaac ctgcatgaaa tgggtggcga gaacgcgccg atcatcatgg
    25201 cgcagctgaa agacgttacc gcacacgcgg cagcgaccgt cagctcctgg ggcttcgtga
    25261 tttcgccaga gcagatcctg caaaccgcga aagacattgg tgagccttct gtcctgaacc
    25321 gtgcaggtgg tgcgccgacg ctggcggtag gtatcgctca cgtgttccac aaagtgctgc
    25381 cgatggctga catgggcttc tggtatcact tcggtattct gttcgaagcc ctgttcatcc
    25441 tgaccgcgct ggatgcgggt acccgttctg gccgctttat gctgcaagac ctgctgggta
    25501 acttcatccc gttcctgaaa aaaaccgact ctctggttgc cggtatcatc ggtactgcgg
    25561 gctgtgtggg tctgtggggc tacctgctgt atcagggcgt ggtcgatccg ctgggcggcg
    25621 ttaagagcct gtggccgctg ttcggtatct ccaaccagat gctggcagcc gtagcgctgg
    25681 tactgggcac cgttgtgctg attaagatga agcgcaccca atacatctgg gtaactgttg
    25741 ttccggctgt atggctgctt atctgcacca cctgggcgct gggtctgaaa ctgttcagca
    25801 ccaacccgca gatggaaggc ttcttctaca tggcaagcca gtacaaagag aagattgcta
    25861 acggtactga cctgacggcg cagcagattg ccaacatgaa ccacatcgtt gtgaacaact
    25921 acaccaacgc aggcctgagt attctgttcc tgattgtggt gtacagcatc atcttctacg
    25981 gtttcaaaac ctggcttgcg gtgcgtaaca gcgacaaacg tactgacaaa gaaacaccgt
    26041 acgttccaat cccggaaggc ggcgtgaaga tctcttcgca ccactaaccg tgtttagccc
    26101 cgcttcggcg gggctttgtt cttatcagag tgaactatgt ttggtaactt aggacaggca
    26161 aaaaaatatc tcggccaggc ggcgaagatg ttgattggta ttccagacta cgacaactac
    26221 gtcgagcata tgaagaccaa ccatcccgac aagccgtaca tgagctatga agaattcttc
    26281 cgcgaacgcc agaacgcgcg ctacggcggc gatggtaaag gcggtatgcg ctgttgttaa
    26341 tggagagagc atgaacccga ttgcagttac cctactcacc ggttttttag gcgcgggcaa
    26401 aactaccctg ctgcgccata ttcttaacga acaacacggc tacaagattg ccgtgattga
    26461 aaacgaattc ggcgaagtct ccgttgatga tcaactgatt ggcgaccgcg ccacgcagat
    26521 caaaacgctg accaacggct gcatctgctg ttcgcgctcc aacgagctgg aggacgcact
    26581 actcgacctg ctggataatc tcgacaaggg caatattcag ttcgatcgtc tggtgattga
    26641 atgcaccggc atggccgatc ccggcccgat tattcagacc tttttctccc atgagatttt
    26701 atgccagctc tacctgctgg acggcgtgat tgcgctggtg gatgcggtac atgccgatga
    26761 gcagatgaac cagttcacca tcgcccagtc ccaggtgggc tacgccgacc gtattctgct
    26821 gactaaaacc gacgtcgcag gcgaagcaga gaaactgcgt gaacgcctgg cgcgcatcaa
    26881 cgcccgcgca ccggtctaca ccgtcaccca cggcgacatc gacctgggcc tgctgttcaa
    26941 caccaacggt tttatgctgg aagagaacgt cgtcagcacc aaaccgcgtt tccactttat
    27001 cgcggataaa caaaacgata tttcgtcaat tgtggtggaa ctggattacc cggtagatat
    27061 cagcgaagtt tcccgcgtga tggaaaacct gctgctggaa tcggcggata aactgctgcg
    27121 ttacaaaggg atgctgtgga ttgacggcga acctaatcgc ctgctgttcc agggcgtcca
    27181 gcgcctctac agcgccgact gggacaggcc gtggggcgat gaaaaaccgc atagcacaat
    27241 ggtgtttatc gggattcagt tgccggagga agagattcgg gcggcgtttg cgggattgag
    27301 gaagtaagtt cgggaaagat caactttttg agagttccac cgcaacatta ttacgagtgg
    27361 aactctttag atatgtcaga ccaaccaaag cttcttcaat tgcgttttca ttttttcatg
    27421 tctcattttc agttcagcca atccccattc tgaagaatta caaacatctt ttgttaaata
    27481 aaaagagctt ttactattaa gtttctcata aatagatttc tttttgataa aatcatagtg
    27541 ttgtgtttca gaatttttgg aaccacttat gagtgttaaa ttacctattt tatgcagcca
    27601 gtagacatgc tcttgaggtt ggaatctatt aatccagtat tcattaacta atgcttgagg
    27661 aagaatatgc tcaattgtga ttctccccgt ataggtttta atcacgctct catcttgttg
    27721 ctccatatct aagcgaagca aaattgcttt tattaagttc acttgattcg gacgaggctc
    27781 atataaatct tcatccagag cagctataaa acctgaatta tctgcatgtt ggtttatctg
    27841 atttatcaca gaatcaaaag gcatatcatt atttattgct accagagcag aataacaaac
    27901 catttctctt tggcttttaa tttgtttttt taaccaccca tgcatatata ccttctcaaa
    27961 ggcggtaata aattgagaaa aatcatctaa attaaaatct tctgtacgag ccattcgatt
    28021 catgaatgcc ataaccggag gaatccactc atctacccct aaattactta gcgatgctat
    28081 ttttcgtcgt attgaagggt gttcaaaatc attctctaaa atcttagtat a
//
LOCUS       DAJVPP010000052        27793 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVPP010000052 DAJVPP010000000
VERSION     DAJVPP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.052"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..982)
                     /gene="yjfF"
                     /locus_tag="OQM34_002204"
                     /pseudo
     CDS             complement(1..982)
                     /gene="yjfF"
                     /locus_tag="OQM34_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(969..1991)
                     /locus_tag="OQM34_002205"
     CDS             complement(969..1991)
                     /locus_tag="OQM34_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1452638.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2005..3507)
                     /locus_tag="OQM34_002206"
     CDS             complement(2005..3507)
                     /locus_tag="OQM34_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1452639.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3584..4531)
                     /gene="ytfQ"
                     /locus_tag="OQM34_002207"
                     /pseudo
     CDS             complement(3584..4531)
                     /gene="ytfQ"
                     /locus_tag="OQM34_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4841..5371
                     /gene="ppa"
                     /locus_tag="OQM34_002208"
     CDS             4841..5371
                     /gene="ppa"
                     /locus_tag="OQM34_002208"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1452640.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5750..6091)
                     /locus_tag="OQM34_002209"
     CDS             complement(5750..6091)
                     /locus_tag="OQM34_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1452641.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6094..9873)
                     /gene="tamB"
                     /locus_tag="OQM34_002210"
     CDS             complement(6094..9873)
                     /gene="tamB"
                     /locus_tag="OQM34_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1452642.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9870..11603)
                     /gene="tamA"
                     /locus_tag="OQM34_002211"
     CDS             complement(9870..11603)
                     /gene="tamA"
                     /locus_tag="OQM34_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1452643.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIPRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11809..12438
                     /gene="msrA"
                     /locus_tag="OQM34_002212"
     CDS             11809..12438
                     /gene="msrA"
                     /locus_tag="OQM34_002212"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1452644.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGVCLPPEA"
     gene            12761..14104
                     /locus_tag="OQM34_002213"
     CDS             12761..14104
                     /locus_tag="OQM34_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1452645.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14166..14372)
                     /locus_tag="OQM34_002214"
     CDS             complement(14166..14372)
                     /locus_tag="OQM34_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1452646.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14697..15251
                     /locus_tag="OQM34_002215"
     CDS             14697..15251
                     /locus_tag="OQM34_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1452647.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15314..16252)
                     /locus_tag="OQM34_002216"
     CDS             complement(15314..16252)
                     /locus_tag="OQM34_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452648.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16464..17204)
                     /gene="cysQ"
                     /locus_tag="OQM34_002217"
     CDS             complement(16464..17204)
                     /gene="cysQ"
                     /locus_tag="OQM34_002217"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1452649.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17394..19337
                     /gene="cpdB"
                     /locus_tag="OQM34_002218"
     CDS             17394..19337
                     /gene="cpdB"
                     /locus_tag="OQM34_002218"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1452650.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19463..19843)
                     /locus_tag="OQM34_002219"
     CDS             complement(19463..19843)
                     /locus_tag="OQM34_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1452651.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            19932..20792
                     /gene="qorB"
                     /locus_tag="OQM34_002220"
     CDS             19932..20792
                     /gene="qorB"
                     /locus_tag="OQM34_002220"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1452652.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            20901..21866
                     /locus_tag="OQM34_002221"
     CDS             20901..21866
                     /locus_tag="OQM34_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1452653.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            21974..22636
                     /gene="ytfE"
                     /locus_tag="OQM34_002222"
     CDS             21974..22636
                     /gene="ytfE"
                     /locus_tag="OQM34_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1452654.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22681..24093)
                     /gene="cycA"
                     /locus_tag="OQM34_002223"
                     /gene_synonym="dagA"
     CDS             complement(22681..24093)
                     /gene="cycA"
                     /locus_tag="OQM34_002223"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1452655.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24401..25021)
                     /gene="fklB"
                     /locus_tag="OQM34_002224"
     CDS             complement(24401..25021)
                     /gene="fklB"
                     /locus_tag="OQM34_002224"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1452656.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25240..25785
                     /locus_tag="OQM34_002225"
     CDS             25240..25785
                     /locus_tag="OQM34_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCS1452657.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25769..26416)
                     /locus_tag="OQM34_002226"
     CDS             complement(25769..26416)
                     /locus_tag="OQM34_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1452658.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26628..27419
                     /locus_tag="OQM34_002227"
     CDS             26628..27419
                     /locus_tag="OQM34_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCS1452659.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27429..>27793
                     /locus_tag="OQM34_002228"
     CDS             27429..>27793
                     /locus_tag="OQM34_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCS1452660.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAR"
BASE COUNT         6837 a         7405 c         6830 g         6721 t
ORIGIN      
        1 tgttgactct tgtcactggc gaactctgac gattctccca cagcaccgtt aacccacgct
       61 gtaatgctat aaaaataaac aacaaaatac cgatggcgat tttcgtccac taggaactca
      121 gcgtgccgtc aaagttgatg taagtctgga tcagtccctg aattgccacg ccaaaaagcg
      181 tcccaagcac cgtcccgacg ccaccactta acaacgtacc gccaatcacc accgacgcga
      241 tggcgtccag ttccacacct acgcccgcca gtgcatatcc ggcttgggta taaatcgaga
      301 agacaatccc cgccagtgtt gccagtccgg tggagagcat ataaatgcga atagtggtgc
      361 tgcgagtaga aatccccatc aggttcgccg acgtcgcgtt gccgccaata gcgtacacct
      421 gattgccaaa acgggtacaa tgcgcaagga aaataccgat aaccaccacc gccagcatca
      481 acagccccat cgcactaagg cgaccgccgc cggggatttt ccacgcaagg cttgagagcg
      541 tgtcataaat gggatggttt atcggaatcg actcttccga aacgagatag ctgacgccgc
      601 gcaaaaagaa catccccgca agcgtaatga taaatgccgg gatcttcagg gcgtcgatca
      661 gaagccccat aaatgcgccg aaggcacagc ccatcaccag cactagcgga aacgccagca
      721 gcggcgagag gccgaaatcg ccaaccactt ttgccagaaa cacgccagta aaggcgatca
      781 ccgaaccgac ggagagatcg ataccgccag agaggatcac aaaggtcatg ccaacggcga
      841 ttatcccaag aaaggcgtta tcggtcagga tattgcagat cacccttgtg gaagcaaaac
      901 cgggaaactg ggtcaggcag tagagatagc ccagcacaaa gacgccgatg gtgatcatca
      961 gcggcaagtt acgttttatc atggctacgt actcctttaa tcagactgat aaagcgttgc
     1021 gactgaacaa tcagcacaca aagcaccacc accgctttca ccacctggtt catctccggc
     1081 ggaaagcccg aaagcaaaat tccggtgttc atcccctgaa taatcagcgc ccccactacc
     1141 gagagcagca ggttaaagcg cccgcccatc agcgatccgc cgccaatcac caccgcgaga
     1201 atggcgtcca gctccagcca taatccggcg ttgttggcat cggcaccgcg aatatccgcc
     1261 gccacgataa tgcccgcaat cgccgcacat agcccgctca acacgtaggt gagcatgacg
     1321 atgattcgcg tgtttacccc ggcatttttt gccgcccgaa tgttgatacc aacgacttcg
     1381 ataaacatcc ccagcgccgt tttgcgggtc aacagccaga acaggagaag cgtcagcacc
     1441 gcgataatga ccggcgttgg caggaacaac agcgatccgc tgccaaacca tgagagatcc
     1501 ggcgagttaa acgtgacgat ctgcccggcg gtgatcagtt gcgccacgcc gcgcccggcg
     1561 accatcagga tcagggtggc gacaaacggc tgaattttga ggatcgctac cagtatgccg
     1621 ttccacaatc ccgccaggat gccggtgccc agggcgctta acaaaacaat cggcaggctg
     1681 aatcccgcga ccgtcatcgc agccgttgtg gctccggcga tagccatcac cgcccccaca
     1741 gagagatcga taccaccggt ggcgatcacc agcgtcatac caatcgccag taacgcaacg
     1801 ggagctgcac ggttaagaat gtcgataggg ctaccgaaca aacgcccatc ctgaagcacc
     1861 acctgccaga aatgcggggc taccaggcta tcgaccagca gcaccagcaa cagtgccgcc
     1921 agctgcggca tccccgtcgg ccagtgaaaa cgcctttttg gcggcgtagt gtcagggaga
     1981 gattgaggca tcacactgtt ctccttacgc cgcaatggcg ttcatgatcg ccggaacgga
     2041 aagctgtgcc agcgggatct ccgccacctg tttgcgatcg cgcatgatga tcacccggtc
     2101 ggcatagccc accagctctt ccagttcgga ggagatcacc agcagcgcca gaccgtcagc
     2161 acacagcgtt tcaatcaggc ggatgatctc ggcgtgggca ccaacatcaa tgccacgggt
     2221 tagctcatca aggatcagaa attgcggtcg ggtcagtagc caacgtgaaa gcagcacttt
     2281 ttgctgattg ccaccggaga gaaattcaat cggttgttca gttgaaggtg tgcgaatgcc
     2341 aagctggcgg ataaagcgtt cggcaatctc ttgctgctct ttgcgggaaa tgggtcgtag
     2401 ccagccgcgc tgggcctgga gagcgaggat gatattttcc cgcaccgagg cggcggcgat
     2461 gatgccatcg gttttcctgt cttccgggca gaatccgatg cccagtactg aagcctgatg
     2521 tggcgatcgc agggtttgcg gtttgccttt gatcaacgcc gtgccgctgt cagcaggttt
     2581 gataccgaag atcacttcgg cggtttcggt acgtccggat cccagcaagc cagccagacc
     2641 gacgatctcg ccggggcgca cttcgagatc aaacggtgct atcgttcctt ttttgccgta
     2701 atttttgaac gcggcaacgg gtttgtcgct caacaatgtt cgcccggcac gctgtagcgc
     2761 gtgggtatcc agctcgcgcc ccagcatcat ttttaccagt tcgatctgcg gtagctcgcg
     2821 cgtttcccga cagcctacga aactgccgtt gcgtaagacg gtgatccgat cgctgacctg
     2881 atagacctga tcgagaaagt gagtgacaaa gatcaggctg acgccgcgat cgcgcaactg
     2941 acgcatcagg ccaaataata gctccatttc ctgggtgtcg aggctggcgg tgggttcatc
     3001 gaggatcagc actttggcgg agagatcgat agcccggcaa atggcgacga tttgctgcat
     3061 cgccaccgaa aaacggttaa gcggttcgcg cacgtcgagg gagaaaccgt aagatgccat
     3121 cagttcggtg gcgcgctttt ccatctcttt acggcgtaga aggccgaaac gtttgggttc
     3181 gcgacctatg aatagattat cagcaaccga catattgggt agcaggttga cttcctgata
     3241 gacggtgccg ataccgagtt gttgtgcatg ggcggtattt ttcggtgaga tagtctggcc
     3301 ttccagccag atggtgccgc gatcggcgtg gtatacacca gttaatgctt tgattagcgt
     3361 tgattttccc gccccgtttt caccaagcag cgccatgatt tcgccacggc gcaggctgaa
     3421 atcaacgttg tctaacgctt tgacgccggg gaaaaattta cttaatcctt cggtgcggag
     3481 gatctcctgg tgttggtcgg tcgtcatggt ttcccccggt gtaaagcgtg ctcccagtag
     3541 gcatgataag acgcggtaag cgtcgcatca ggcatagcaa ccctcaatac cccatatttt
     3601 tcttcttctc taactcttct tttgcggtat caggcaggta aagggtggat ttggttaacg
     3661 tcagcttttc aggcatggtg ccgtcttttt tgtatttctc cagcgcatcg aaagcgggtc
     3721 ccgccatgtt cggcgtcagt tcaacactgg cgttcgcttc gccatccatc atcgctttgt
     3781 agatgtccgg tacaccgtca atggaacccg tgaggatatc tttgcccggt ttcaggcccg
     3841 cttctttaat tgcctgaatt gcaccaatca ccatgtcgtc gttatgggcg taaaccatgc
     3901 agatgttttt gccgttgttt tccgctttga taaagctctc catgacttct ttgcctttac
     3961 tgcgggtgaa gtcacctgac tgcgagcgga taattttgat atttggcgca ttcttaatgg
     4021 cttcggcaaa gcctttctta cggtcaatgg cgacgctggc cccaacggtg ccctgcagct
     4081 ccaccacgtt gcatggtttg ccattcactt cttttaccag ccagtcaccg atcaacttac
     4141 cttcgaggat gttgtcggca gtaacggtgg tcatatagag agatttgtct ttcacatcga
     4201 tggaacggtc gagcaagaag accgggattt cggcatcttt cgcctctttt aataccggtt
     4261 cccaacccgt cgcaaccact ggagcgataa agatcgcatc caccccttat gcaacgaagg
     4321 aacgtaccgc tttaatctgg ttttcctgct tttgctgacc atcggcaatt ttcaacgtaa
     4381 ttccgcgttt ttcggcctca cttttcgcca cattggtttc tgcggcgcgc cagccggatt
     4441 ccgatccgac ctgcgaaaat cctacggtta atggagcggc caacgccata gacgacatgg
     4501 ctgccgagac tacaagtaag cgtttccaca tagggttgtc ctcgtcgggg taattattat
     4561 tggtgaaaag atgttcgcga aaaaactata gacaattcgt tatgtaacgg attgcgttac
     4621 atcacacttc aaaaaagtaa ataaaacgtt aatcacaagt ttgtaatcgc tttcatctca
     4681 ctatgaaaaa tgcggctacg gttatggatt ttcctgctct gtataccgtc ttaaaactgg
     4741 cgaaaaagga aaatgaagac gaaaacaagc aaagacattc ggcgcgagtt ggctataata
     4801 cttggcactt gtttgccaca tatttttaaa ggaaacagac atgagcttac tcaacgtccc
     4861 tgcgggtaaa gatctgccgg aagacatcta cgttgttatt gagatcccgg ctaacgcaga
     4921 tccgatcaaa tacgaaatcg acaaagagag cggcgcactg ttcgttgacc gcttcatgtc
     4981 caccgcgatg ttctatccgt gcaactacgg ttacatcaac cacaccctgt ctctggacgg
     5041 tgacccggtt gacgtactgg tcccgactcc gtacccgctg cagcctggtt ctgtgatccg
     5101 ttgccgtccg gttgccgttc tgaaaatgac cgacgaagcc ggtgaagatg cgaaactggt
     5161 tgcggttccg cacagcaagc tgagcaaaga atacgatcac attaaagacg taaacgatct
     5221 gcctgaactg ctgaaagcgc aaatcgctca cttcttcgag cactacaaag acctcgaaaa
     5281 aggcaagtgg gtgaaagttg aaggttggga aaacgcagaa gccgctaaag ctgaaatcgt
     5341 tgcttccttc gagcgcgcaa agaataaata agttcttcta gcgcaataac cctgaacgcc
     5401 gggcttcggt tagtaagggt ttttttatgc ccgcgataaa taaactctct attccaccat
     5461 cattattctc agcggttgca aggcttgaac ggtaagaaca agcaaacccg accaccattt
     5521 tgctgttcat agccacttgc tggaagttag ccgacctcac tcatactcac cgttcaaagc
     5581 gtgcccccgt tttacccctg cacatttgtg aacgccagca gagtttttta tgcgattgca
     5641 gatatggaaa gcggctcgga gagaaaagca aaagatggaa aagttacaaa aaaaatcgcg
     5701 agatacatcg cataaaacaa cgccacccga aggaggcgtg cttttattat tacttatccc
     5761 tgtctaacca gtcgccgctt tcaattagct ttaatccatc gacgggtcgt tgataaacgt
     5821 acatccatgc actcccatac ggcgtctgaa tcaactggcg cgcgtattca ccgcccctgg
     5881 tgcgcaaggc atcaagttcg gccagcgtgg cgttgtcaat acgataaact tcaccgtgta
     5941 ccgttccgtt ccccggaact gcgcctggat agtggcccag gctatacaac tggtagttat
     6001 cgatactgaa atcgcccagt aactgggcat tggtcatcca gtgactgttg ccttgtttgt
     6061 ggcgtaaact gccgtagaca aatattcgca ttgctaaaac tcgaactgat agagcaaatc
     6121 cagtgcctgg tctacaccag acacggcttc cagatatagc ttaggcatca ggcgataacg
     6181 taacgtgagt gttgctatag agtcaaatat acccacgccg tatttcactt gcagacctgg
     6241 caatacatag ccgctgacca ctacctggga ggagtcgcct actcccaggg tgtcgagcgc
     6301 taaattgctt acgccaaacg tctcgccgat tttacccaca atctggccac tttgcgcaac
     6361 ccccagacca atcagcatcg aggtcattgc cgcactgtcg ctctgatcgc tctccagccc
     6421 ttgtccacgt agcaaataag acaatgcagc ttgttgcgac atcgccgggt cagagaagat
     6481 ctccgctttc ggttcgtccg ccagaccagt gacgcgaact ccggcgatta catcgtcttc
     6541 tgtagcatcc gggttacgaa tagcttcaat attaagatac ggttgatccg gcggaccaga
     6601 gaacagtaac tcacccttac gcacaatcag atcctgacca taggcatgga agcgcccttc
     6661 agggatgttg atctgcccgt tcaggcccag cccttgtttg tcctgtacga cgttaagatc
     6721 gcccgtcagc cgcgctttca ggccaaaggc gtcaatgcgc acattgttgc caacgtggac
     6781 aatcaggtta ctgttaatcg gaatcgacgc cgttttcggc tcttccggtt gcaggttatc
     6841 gttaagcatc accacatcgc tggagacgcc tactgcgctt tccggcagat cgtgcaccac
     6901 gatgcgcgcc cacgggacat ccacgcgacc atcgagggta aacaggtttg gtgtagcctc
     6961 gaatacaaca tctggcgata catccattcg taccatcggc ggcacggtga tccgcacttt
     7021 actgcctttc gccgttactc gtgcccgcca gttttcaatt tggctccagt cggcattacc
     7081 gttcaggtag atttcaccct gctgggtccg tactgtaccg gcaagcgtcg agcgcatacc
     7141 gttaaagttg accgcaagct ggctcggctg catatcaaac ggcataaagt tgccgtcgat
     7201 atccacaccc gtaacctgaa gctgaccaaa caactgcggg ctttgcacat caccacccag
     7261 acgcaagttg gcactcacca tccccgctgc tttttcccca cgggtaaaga tggggtttat
     7321 catcgcaagg ttgaagttac ggatattgac gttgccacca agattacggc ggccttgcgg
     7381 atcggtcacc tgcacctgtc catcaaactg gccgttattg gtcaggcgga tggtccagcc
     7441 caattcggca cggttgttac gcaattccgc cgtcagattc agtgtctgaa acgccaccgg
     7501 cagcgccgca tcgttgacgg tttgcgttac ctgcacgtta cgccccgaaa gggtgatact
     7561 gccctgcggc agcccctctt tcgtggtgtc ccaggcaaca tccgctttac ccgtgaagat
     7621 accgctggcc tgagtggttt ctggcataaa cggtttcagc atggcgaggt cgaagcggtt
     7681 gagattcacc accgcacgcc cttcggcccc cgcatcgata gtttgcggca cgcacagttc
     7741 cgcattcggg ttaagccagc agtgtggccc gatgctgatt ttttgctcct tattgcggta
     7801 atccagcgca atatcgcggg tcagcgacca cgggccgacc ggcgtctgga agcgggtatt
     7861 gctaagagtt cccttccagc gttcttcttt gcgatcaaaa cttcctgcca gattaagctg
     7921 cccggagaca ggctcgccct gaatccgcaa ctgtagctcg tgctgctttt cgctgccttt
     7981 ggcattcagg gtgacgaggt tgatatttac atccggctgc gaaatttgct caacgcgtac
     8041 gtcgagtttc ccggcgatct gatcggtgga tttaatgtcg ccttccacgc gaacctgcgc
     8101 cacggaaagt tcctgccagc gcaggccgcg cgcggtgata tctgccagta gttgtggcgc
     8161 ttccaccgtg ccgcgtactt tcaccagccc tttcgctgta ccgccaagcc ccggcagcgc
     8221 gttatccagc cccggcgcgt tgatggtggc atcaagattg agatctttta cccccagctc
     8281 gcctttcact tcggcactgt ttggcccgag ttccagatga agcccaggga tcatccactg
     8341 catataactg ttgcctttca gcgtgccgtc aacgttcact ttgttctgtt tgacgttacc
     8401 ggtcagcttc agttctggca cctccatctg ccaggtgccg ccgtacaggc taccgcgggt
     8461 tttaatcaag ccattgagtt tcgacggcca ctccgggaac tctttggcgg tgttaatgcc
     8521 attaagcgtt agctcaccgc gccaactaat ggcctgctgc cagtcgagca acgccttgag
     8581 ttcagttttc ccttccagcg ccgcgacggt gagtttgtcg agattgacct gctgttcatt
     8641 acctttggca tcaagggtaa tggtggctgg cgggatctcc tgtcccttca ctgccgtacg
     8701 catagagagc gtgtaatcgg tcattttgcc ggtaagtttc agtttcagat catccgcctg
     8761 atactgcttc tcaccagtga acggccagta aagctgtttg ctgttcactt ccacgttgag
     8821 tggcaatccg gcttccgcca gtcgcgtctg ggcgcgtaaa tccatatcca ccggaccgga
     8881 aaggttaacg cctatctcca gctgttcgcg cagcgcgccg cccactttca gcttcacctt
     8941 ttcacctttc aacggctcca cgttcagtgt gctgttgagg gtgatatcca ccggccagtt
     9001 gtctgacagc tgcgccgtgc cgctggcgtt gacgatcccc tggttggaat cgatatccag
     9061 ggcgtccagt ttagtattgc cgtcaatgct gctcactttc agcagcatgg tgcgcacggt
     9121 gatgtccgtg tcgcccgtca cgcgcagctg ctcgccctta aactcttcaa tgttcaggtt
     9181 aagcggcaaa tgcacgtcgg tcatttccgg caataccggg cgagaaaaga gatctttcag
     9241 cgtttcgccg agcggctttt cctccggctg cggattttca attttcggtt cgaccacttc
     9301 ttcctgcgcc acttccacca ctttcggcag agcaatcagc aggcctttca gcgacgtcgg
     9361 tttcagggtc agggttttct cctgccagtt caggccggag gtgaagtcca tcaccgacac
     9421 cgtggtgtca tcaatcttga tgttgacgtt gtccagcgcc acccgtgtca gggtgatggg
     9481 atacggcgtg gagagatcca gcggaccgct atcttcctct tcttcaacct gttcagaagg
     9541 aggcattttt ttactgtcga tgttgacctg aatgtctttc agcgccaagt cattaataca
     9601 aacgctactg ttccacaggc actcaagacc gacccccaga tgcagattac ccgcttttac
     9661 cgccacgcct agctgctcat aacgaacgtc agacaaggtg agatcgcgcc agccgccggt
     9721 gaccttgcca atatccagtc ctggcaccca gcgatccgcc gctttaaata caagatgcag
     9781 gccgctggtg gtgcccacca gaaacgccac cgatcccagc aacagtaaga taacgataac
     9841 cacgccgagg ctgatttttt tccataaact cataattctg gccccagacc gatgtaaaac
     9901 tgtaacccgt gttcgtcttt atccgcgacc ggtacggcaa aatcgagttt gattggcccg
     9961 accggcgatt cccagcgtac gccgaccccg gtaccggttt taaagtcgct gcggggaata
    10021 tcgcttaccg cttcgccact atcgacaaac accgcgcccc accattttcc ggtcacgttg
    10081 tactggtatt ccagcgatcc ggttatcaac ttcgaggccc ctttcaggtc accgttggcg
    10141 tatttcgggg cgatagattt gtatttgtag ccgcgaatac tgcgatcgcc cccgacgaag
    10201 aaacgcagat ccggcggtac tttgtcgaaa tcaccggttt caatccagcc cagcgtgccg
    10261 cgtgtcacaa agcgatggcg atcgtacagt gtgcggatcc agacgtgctg cgcctggaaa
    10321 acggagaaat cgacatctga accccaggcc gtgttggagt agtcgataga gtagcgttgc
    10381 gagtctcccc aggttggcat caggccacca cgagaacgcg tgcggctaat catcacccca
    10441 ggataaaaca gcatcgtggt gttggtaatt tcaccctggg taaagtggtc gaggctccag
    10501 cgcaggttaa tggcacgctg ccagccgctg gagagatccc agtagcgaga agccaccagc
    10561 gtagtggagt cagattcggt atcgttcagg tcagtgcgct taaaaccgcc ctgcaccaaa
    10621 taatattgtt ccagtggatt cttcagcagc ggcattttat agctgaagtc gagggtctgt
    10681 tccggcgcgg aaatactggt actggtggtc aaactgtgac cgtatgagtt catccacggc
    10741 tttttccacg tcgctttcac gcgcggtccc acgtccgtag agtaaccgac cccggtttcg
    10801 atggtgtttt ctgttcgcgg cgaaaccacg cccgtcaatg gtaatacttt cgtttcgcgc
    10861 gctttatcaa attgtggagc caccaccacc gagttaaacc agccggtagc agaaagtcgg
    10921 cggttcagtt ccgccagatc tttcgattca tactcatcgc cctctttaaa cggcaccaga
    10981 ttttgcaggt attcatcgcg gatttgtgat ccttcaaagg tcacatgccc aaagcggtaa
    11041 cgttcgccac tgttataatc aatatcccag aaggctttat gcaggccgag cgcaatgccc
    11101 agctgcgctt tggtaaattc gctatcgaaa taacctttac gcaacgcaat actggttaag
    11161 gactttttga aattttcata atcgccctgg ttcagtaccg taccaatagc cgggcgagta
    11221 tcgagcaatt tcaaatagtc tttatcggtc cgcgcgccgc cgcgcaatac cacatcggtg
    11281 ccgccaatta acaccggcac acctggcgtg actttggcga tcaatacctg ccgccctttc
    11341 tttggcggtg gacggagatc aaattcaatg gtcggctggt aataacccag cgctttcaga
    11401 ccttcacgga tggcatcatc gacgcgtgcg cgaaagcgac ggtctggcgt cacttcatca
    11461 ctttcaatcg tagaaagctg cgcacgaacg ttcttttcca gctgtcccga taacccctcg
    11521 acctgtagac ggacgttcgc ggcgacggca gatccgctta agcagagtaa gcttacacag
    11581 cataactgtc ggatatagcg cacattttct cctgaatatc cttttttcct gcccctggaa
    11641 cgccgttaaa cggcttaaca aaaatccagt aatatggatt aaaaagcaga ctaaacccca
    11701 aatatttctt atgttttact ttagacctat tcacggtggt tattgtgtgc aaatacgcct
    11761 cttgttacaa ccttaacccc aatgaccgat tttcgggaga gcgacaccat gagtttattt
    11821 gataaaaagc atctggtttc ccccgccgat gccctgcctg gacgtaacac cccgatgccc
    11881 gtagccacgc tgcatgcggt caacggtcac tcaatgacca atgtacctga cggaatggag
    11941 attgccattt ttgcgatggg ttgtttctgg ggtgtggagc gtctgttctg gcagttaccc
    12001 ggcgtttaca gcaccgccgc aggctatacc ggcggctata cgccaaatcc gacttatcgg
    12061 gaagtgtgct ccggtgatac gggtcatgcc gaagcggtac gcattgttta cgatccttcc
    12121 gtcatcagct atgagcagtt gctacaggta ttttgggaga atcacgatcc cgcccaggga
    12181 atgcgtcagg gcaatgacca cggcacgcag tatcgttcag cgatttatcc gctgacccca
    12241 gaacaggatg ccgcagctcg cgccagtctg gaacgttttc aggcggcgat gcttgccgcc
    12301 gatgacgatc gtcacatcac cacggaaatc gctaacgcca caccgtttta ttatgccgaa
    12361 gatgaccacc agcaatatct gcataaaaac ccatatggtt actgtggaat tggcgtctgt
    12421 ctgccgccgg aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg
    12481 tctatgcctg atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat
    12541 attgcaagga ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag
    12601 cagtctgaat tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa
    12661 aatgccggtt cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac
    12721 tttcccttcc gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt
    12781 catactctgc ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc
    12841 ctcacgcaaa atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt
    12901 tctgaatatg caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc
    12961 agtggcgatt ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct
    13021 gttctcccgc tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc
    13081 gttagtgact ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat
    13141 tgcgccagaa gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg
    13201 caccccgctg gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact
    13261 gccaatggta cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg
    13321 tgcgctggcg ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct
    13381 ggaatcccgt accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct
    13441 ccacgaagat gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct
    13501 cgtctgtaat gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa
    13561 ccgcgtgctg gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct
    13621 gattgtgccg gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga
    13681 agacttcgcg gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga
    13741 cgtgatgacc acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc
    13801 ccgtgatgag aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt
    13861 gctggatatt gacgagttcc cgcagtcggg caactacgaa accatcggcg gctttatgat
    13921 gtttatgctg cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt
    13981 tgaagtggtg gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag
    14041 caaggccacc gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc
    14101 gtaaccccca gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac
    14161 tttggttaag ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact
    14221 gacaaatgca gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa
    14281 gcgccaatgt ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc
    14341 tgaagtgggt tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg
    14401 tgcggtgcca caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata
    14461 gactatccgg gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt
    14521 cagaattatc tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt
    14581 tgtgcgcttg accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg
    14641 attaaaactc agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga
    14701 ccctacgcaa gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc
    14761 agttcgaaat cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc
    14821 ttgataaaga tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc
    14881 gagtactgca acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg
    14941 aagcgattaa atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca
    15001 ccgacgacgc gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa
    15061 agctttatcc ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc
    15121 agctggatga agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg
    15181 ccaaagacgg ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt
    15241 tgctcaaata atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta
    15301 tgggataggg gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt
    15361 gctctttttt aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa
    15421 tattactatc tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga
    15481 cttgttggca acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca
    15541 aaacaacaga ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc
    15601 ccaaagattc tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc
    15661 catcgcattc tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt
    15721 tgcttactac atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa
    15781 tataaccttt aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat
    15841 acaatcttct gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat
    15901 actcatttat tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg
    15961 tatattcagc accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa
    16021 cagtttcatt ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac
    16081 gaaaggactc ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa
    16141 atgcatgctg aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta
    16201 caaataactc caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac
    16261 cttattttat ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc
    16321 aaaaaacgct cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac
    16381 aatctctttt tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa
    16441 tacactgttt ctgccatctg aatttagtaa atggacactc tgaaccctgg attcaggaac
    16501 gactcgcgcg gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca
    16561 gctgcagcca cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg
    16621 tacagctgcg cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg
    16681 gtctgatgtt cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg
    16741 ctgataacca ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac
    16801 tcttctttcc aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac
    16861 accacaccta aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca
    16921 ttacgtttaa taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag
    16981 tgctgacgga cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc
    17041 agcgtacgta aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga
    17101 gaattgtccg ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg
    17161 gcatcgcctg cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc
    17221 tgtgagcggt gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg
    17281 tgatagtgtc atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa
    17341 ggcgtttatt ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta
    17401 agtttagcgc aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg
    17461 atctgcgtat catggaaacc actgatctgc atagcaacat gatggatttc gattattaca
    17521 aagacaccgc cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc
    17581 gcaatgaagt gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc
    17641 tggccgatta catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg
    17701 cgctgaatac gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc
    17761 tggattacct gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca
    17821 ttgacgccag aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg
    17881 tcgataaaga cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc
    17941 aaatcatggg ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg
    18001 aaaccgtgcg caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc
    18061 tggcgcattc cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt
    18121 acctcagtga aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc
    18181 cgggtaaaga ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg
    18241 ttccggcggt aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca
    18301 gtaatgacag cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg
    18361 acatcgccaa taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag
    18421 ccgatcacga tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata
    18481 tgtatagcta tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc
    18541 aaaaagcgta tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc
    18601 tttcagctgc cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg
    18661 aggtggaaaa aggccagttg accttccgta atgccgccga tctttatctc taccccaata
    18721 cgctgattgt agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg
    18781 gacagtttaa ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg
    18841 gtttccgcac ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc
    18901 aacccgcccg ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga
    18961 acctgacctt taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata
    19021 actatcgcgc ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt
    19081 caccggatga gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg
    19141 gggaaattca tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga
    19201 aactggatat ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga
    19261 aagggcagta tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg
    19321 tggatttgag taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt
    19381 attggcggat gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg
    19441 ataagcgcag cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg
    19501 gcaaattcaa ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca
    19561 gcggcgtgag gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa
    19621 aaccatcctg ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga
    19681 ttttgcgccg caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca
    19741 ccccccaacg gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga
    19801 gattaccctc tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt
    19861 gcacgtactt actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat
    19921 ggagacttcc catgatcgct attactggtg ccactggcca acttggtcac tatattattg
    19981 aatccttgat gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa
    20041 aagcccaggc cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag
    20101 ccgcactgac atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag
    20161 tgggtcaacg tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga
    20221 aatttatcgc ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg
    20281 agcacatcga gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca
    20341 acggctggta cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat
    20401 ttatcggtgc ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag
    20461 ctgcggcacg cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg
    20521 atagtgcctg gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg
    20581 ttacctatca aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc
    20641 ccgacggact ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt
    20701 ttgataacag caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa
    20761 gcgtaagcca tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc
    20821 tctctgataa tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga
    20881 cagaccaatg gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga
    20941 tgtgggggct tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt
    21001 cgatggggcg ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg
    21061 tgcgtctgcg tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg
    21121 gcaacctcat ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt
    21181 ccacgatgat tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata
    21241 gccagcgcga cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca
    21301 tcggcctggc gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg
    21361 cacgttatac ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg
    21421 ccctgcgcaa cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga
    21481 cggcgcaggc gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact
    21541 ggctgaatat acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta
    21601 ttagtctgat ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg
    21661 tcgccagcca gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg
    21721 caggtttgct gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg
    21781 gtatcgcgct gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt
    21841 taactgaatc tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac
    21901 gtaagggtta tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa
    21961 tgaggtatca gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc
    22021 tcgcgcttcg gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac
    22081 gctggcgcgc gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc
    22141 aaagcttgct gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat
    22201 tgaccatatc atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct
    22261 gcaagcgact aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac
    22321 aaaatacctg accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat
    22381 cctcttcccg atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat
    22441 ggaaagcgag cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa
    22501 cgtcacaccg ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga
    22561 actgattgat gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc
    22621 cctagcgggt gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg
    22681 ttatttccgc agttcagcag cccgcttctt accaataaac agccagccca accccagcgc
    22741 gataaaccac agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa
    22801 cacgaccacg aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat
    22861 cttgtagatc gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca
    22921 aaggataatc gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa
    22981 cgcgccaatc acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca
    23041 cgagaaggtc aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg
    23101 cgcaacacct tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt
    23161 agcggaagac gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa
    23221 acctaccaac acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg
    23281 tgtcacggac ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat
    23341 ggagttaatc gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc
    23401 taccagctca atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact
    23461 taaacctttc gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc
    23521 ggtcggtgac tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat
    23581 ggcgacgatt ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc
    23641 aaggttgagc gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc
    23701 ggggaaccag aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac
    23761 ccagcagaac cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga
    23821 agcgaagtca ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg
    23881 catcacgaaa aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc
    23941 aaggctaatc gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc
    24001 aataagctga acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg
    24061 atcatcggca acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt
    24121 tgttcaggct ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat
    24181 cgatattagg taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat
    24241 gtttaaagtg agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt
    24301 acggggtctt tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat
    24361 gcctgctccc ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt
    24421 cgacttcaaa caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat
    24481 atgccagttc ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg
    24541 cttcaatcca gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa
    24601 cggagctgtc aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt
    24661 cggtacgtgc cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt
    24721 cggtgctatt cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag
    24781 ccatcgcctg gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg
    24841 cgcgatgcac cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac
    24901 ctgcaaccag tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta
    24961 cctgcaaacc aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca
    25021 tcgtatttcc tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg
    25081 gtaaaacttt gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc
    25141 ccgtatgaat ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag
    25201 gctatgattg aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta
    25261 aaaccaaccc tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg
    25321 ccgccgatgc atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg
    25381 cttccatctg atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt
    25441 cagtcgcaat cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc
    25501 cagccagata gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg
    25561 atggcgcagc tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag
    25621 gtagaaggcg cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc
    25681 cagaacgcca gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc
    25741 ctgtttaccc gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca
    25801 aaagccagct aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg
    25861 aaaccatact gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc
    25921 caagcgcagc aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt
    25981 atttcttccc ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta
    26041 agagacgctg tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt
    26101 ttaaaaacca aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg
    26161 ctttttgtgg cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc
    26221 aggtatattc cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg
    26281 tctgtttact acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat
    26341 attcggcaaa ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc
    26401 tggtatcaat atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat
    26461 ttgatttcag cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc
    26521 ctgtgatcgc catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca
    26581 agctaatcat caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc
    26641 gtcctgttgc cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc
    26701 ttgctcgtgg cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa
    26761 aactgcatat cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg
    26821 ctgatgtcgg tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg
    26881 ggcggctgga ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc
    26941 tcgacctcga accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct
    27001 tccttgctca ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc
    27061 cgcaccgctc gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg
    27121 gcgaatacac cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc
    27181 tttgtaatga acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga
    27241 ctattccggc aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac
    27301 gttggggtta tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga
    27361 tttacacctg tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag
    27421 gctgaatcat gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac
    27481 tgggctataa aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg
    27541 caattgcccc ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc
    27601 ttgccgccag taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga
    27661 cactggcgga acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg
    27721 tgcttaacgc cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc
    27781 tatatgaagc gcg
//
LOCUS       DAJVPP010000053        27484 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVPP010000053 DAJVPP010000000
VERSION     DAJVPP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.053"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..358
                     /locus_tag="OQM34_002229"
     CDS             <1..358
                     /locus_tag="OQM34_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1452661.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            620..982
                     /locus_tag="OQM34_002230"
     CDS             620..982
                     /locus_tag="OQM34_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCS1452662.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            979..1893
                     /locus_tag="OQM34_002231"
     CDS             979..1893
                     /locus_tag="OQM34_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1452663.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRMTAEWFYKLHNKISNPKIEENVGDFRLMS
                     RGVVENIKLLPERNLFMKGVLSWVGGSTDVVEYARAERVAGSTKFNGWKLWNLALEGI
                     TSFSTFPLRMWTYIGLFVAGLAFLYGAWMIIDTLAFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYTEVKQRPRYLLKEK"
     gene            1895..3208
                     /locus_tag="OQM34_002232"
     CDS             1895..3208
                     /locus_tag="OQM34_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452664.1"
                     /translation="MAERIRYVFLAIFCALLCKESIDIWMMNNGDFDRAITPFMNGIR
                     QFSHDGTLLYTLKENFSSIVNYEYKSSFSYILYLYAYVISIFTHTFDLRLWSSLSKIV
                     YICALYKLFVEINNKKYSYLLFPVSALLLFTPSILSQFTAFYQEQIVITLLPVITYII
                     LKKEISRFDKVLLVACAALIATSKSQFFYIPILISLISIIFIDIRDKKFHLAMVIATI
                     IALSFSFFSPSATKYNSYHSLYFGTLLYNKSNGKENPSWAVDECIGVDAWGNKFDLDK
                     GAVTTKDAGACFAKNKERGLKDSLLLMLKQPSMFLLLPFDSGVRTQLTEDYFHVFKEN
                     KLIISKSVFLNEVQKIKDSALSTVRIPVAFIAMIASLIFIRKKYSVAIFTLSAFSISQ
                     FYISFIGEGYRDLDKHLFAMNFSFDLMIFIVLSIILDKVASGIYK"
     gene            complement(3243..3722)
                     /locus_tag="OQM34_002233"
     CDS             complement(3243..3722)
                     /locus_tag="OQM34_002233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452665.1"
                     /translation="MTLEMRGNTCLGVGYTASASRPDVSISGYSANEQAIFIDYDNLW
                     IPSQSPVVDATVKWKSSPDFAFGVSVVGATGAMGNRRNILSVTRGATGVYTVNANHDI
                     ANVAVEITTVGVPGYGIIGGYPTANSFVVRMYNTAGAPTDIDFIALGKDYRARFNAT"
     gene            3881..5027
                     /locus_tag="OQM34_002234"
     CDS             join(3881..4131,4130..5027)
                     /locus_tag="OQM34_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1452666.1"
                     /translation="MSGKRYPEEFKIEAVKQVVDRGHSVSSVATRLGITTHSLYAWIK
                     AYGPDSSTNKVQSDAQAEIRRLQKELKRVTDERDILNLQRGVLRKAVRLRYAFIRDNT
                     HCWPVRLLCRVLDVHPSGFYAWLQQPDSRRHHADLRLTGLIKQFWLESGCVYGYRKIH
                     LDLRDTGQQCGVNRVWRLMKRAGIRAQVGYRSPRARKGETSIVTPNRLQRQFNSEAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKVIGWSMQPRMTKDIVLNALLMAVWRRNPQ
                     KQVLVHSDQGSQYTSYEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARGDIFDYIEMFYNSKRRHGSSDHMPPTEYENQYYQRLGSV"
     gene            5044..5559
                     /locus_tag="OQM34_002235"
                     /pseudo
     CDS             5044..5559
                     /locus_tag="OQM34_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(5531..5950)
                     /locus_tag="OQM34_002236"
     CDS             complement(5531..5950)
                     /locus_tag="OQM34_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1452667.1"
                     /translation="MSYFYSASTNGFYSTEFHGTNIPDDAVEISESEWKTLINSQSVT
                     KMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQDAVDLGMATDD
                     ETKLLLAWKKYRVLLMRVNTSTALDIEWPTPPAVQAR"
     gene            complement(5955..6605)
                     /locus_tag="OQM34_002237"
     CDS             complement(5955..6605)
                     /locus_tag="OQM34_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452668.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPM
                     TFSRVLAVTVTLNVTGAAGQPTVSATNVHNTGFNITVSPGSGYGASSDAYYIAMGY"
     gene            complement(6609..7193)
                     /locus_tag="OQM34_002238"
     CDS             complement(6609..7193)
                     /locus_tag="OQM34_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCS1452669.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSARDPAIAGAAPSLTRVHQRAD
                     ALMRELDPHTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPVATNTTWMTCGD
                     PCDSALRFWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(7184..8242)
                     /locus_tag="OQM34_002239"
     CDS             complement(7184..8242)
                     /locus_tag="OQM34_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1452670.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSTGGVLRVPIACSSAGAVGNADDGTSLILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIAGSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHKVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            complement(8229..8654)
                     /locus_tag="OQM34_002240"
     CDS             complement(8229..8654)
                     /locus_tag="OQM34_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCS1452671.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(8654..9202)
                     /locus_tag="OQM34_002241"
     CDS             complement(8654..9202)
                     /locus_tag="OQM34_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCS1452672.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     IEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPKARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(9202..10281)
                     /locus_tag="OQM34_002242"
     CDS             complement(9202..10281)
                     /locus_tag="OQM34_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS1452673.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(10278..11654)
                     /locus_tag="OQM34_002243"
                     /pseudo
     CDS             complement(10278..11654)
                     /locus_tag="OQM34_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309364.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
     gene            complement(11679..13586)
                     /locus_tag="OQM34_002244"
     CDS             complement(11679..13586)
                     /locus_tag="OQM34_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1452674.1"
                     /translation="MADNFQLKAIITAVDRLSGPLKGMQRQLKGFQKEVSSLALGAAG
                     AGTAIMGALALPVKSAITLESKMADVRKVVDGLDTPDAFKAMTEQVRALSTELPMSAD
                     GIAEIVAAGGQAGIARDELMQFATDAVKMGVAFDTTAEESGQMMAQWRTAFNMTQDEV
                     AGLADKINYLGNTGPANAKKISDIVTRIGPLGGVAGVASGEIAAMGATIAGMGVESEI
                     AATGVKNFMLSLTAGNSATKSQKQALRFLRINPKKLAADMQKDARGTMLSVLDAMAKV
                     PKEKQAAVLNALFGKESLGAIAPLLTNLDLLRTNFRRVADSQQYGSSMQKEYASRAAT
                     TENQLLLLQNQLDAISSTLGETFLPEVNDGLEAVKPLLEEVRTFVRENPELVKTIAKI
                     GLALLTVGAAAGSLSRIMKVLGGVMNMTPAKGLIALLVGGAYLIIDNWETVGPVIKKV
                     WHVVDETAQVMGGWETVLKAIALFMATKWVADVTKSITAVTREMRTLGKVSAETGLMG
                     KGRGFIGKAGVYGFLGTLMYEPVKDTLESVVPEDTVNWLDNKGLFLASDWTPFFDRKE
                     YEQYQASLSQYKPNVPLLNPSSSMTQHSELKVTFENAPLGMKIIDVPGKADPLMKITH
                     DVGYSPFRFPR"
     gene            complement(13671..13994)
                     /locus_tag="OQM34_002245"
     CDS             complement(13671..13994)
                     /locus_tag="OQM34_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1452675.1"
                     /translation="MIELVLKKPIIAHKETLHVLEIREPTYDEIEALGFPFSVSPDGG
                     MKMDSQVALKYIPLLAGIPRSSAAQMTKLDIFKAGMIVMRFFTGLETEESSGSDSTMS
                     RGSGN"
     gene            complement(13991..14347)
                     /locus_tag="OQM34_002246"
     CDS             complement(13991..14347)
                     /locus_tag="OQM34_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS1452676.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(14347..15843)
                     /locus_tag="OQM34_002247"
     CDS             complement(14347..15843)
                     /locus_tag="OQM34_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS1452677.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGTGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATV
                     TLTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIKDAINAVPALPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKDTQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVLERDASDPNRLNTLFPPDYVNQLRVFAVVNQLRLQYSEESA"
     gene            complement(15827..15997)
                     /locus_tag="OQM34_002248"
     CDS             complement(15827..15997)
                     /locus_tag="OQM34_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCS1452678.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(16006..16566)
                     /locus_tag="OQM34_002249"
     CDS             complement(16006..16566)
                     /locus_tag="OQM34_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452679.1"
                     /translation="MKLTPVIAALRARCLYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEIPLPS"
     gene            complement(16563..17087)
                     /locus_tag="OQM34_002250"
     CDS             complement(16563..17087)
                     /locus_tag="OQM34_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452680.1"
                     /translation="MDQTAFLHVDFKQPEEMEFNRARLRRAFVQIGRVYMRDARRLVM
                     RRGRSAPGENPGYQTGRLARSIGYYVPRKSSRRSGLMVRISPNQKNGQGNRRFPEGSA
                     YYPAFLYYGVRHAAYGMSKKDKRQKKQHSSRWRLAPRNNFMADVIDQRRYWTQKLLSR
                     ELQRSLRPVRRKKT"
     gene            complement(17059..17469)
                     /locus_tag="OQM34_002251"
     CDS             complement(17059..17469)
                     /locus_tag="OQM34_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1452681.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDRVYRVKRQR
                     DLNGARRFLLLECTELGAEEQMGGRSGSDSIFTR"
     gene            complement(17466..17789)
                     /locus_tag="OQM34_002252"
     CDS             complement(17466..17789)
                     /locus_tag="OQM34_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCS1452682.1"
                     /translation="MLLTMEEIKAQLRLDEDFDVDDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(17792..17992)
                     /locus_tag="OQM34_002253"
     CDS             complement(17792..17992)
                     /locus_tag="OQM34_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452683.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(18041..19246)
                     /locus_tag="OQM34_002254"
     CDS             complement(18041..19246)
                     /locus_tag="OQM34_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCS1452684.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGDATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAVA"
     gene            complement(19261..19911)
                     /locus_tag="OQM34_002255"
     CDS             complement(19261..19911)
                     /locus_tag="OQM34_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1452685.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            complement(19889..21130)
                     /locus_tag="OQM34_002256"
     CDS             complement(19889..21130)
                     /locus_tag="OQM34_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1452686.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRAAGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(21130..21312)
                     /locus_tag="OQM34_002257"
     CDS             complement(21130..21312)
                     /locus_tag="OQM34_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452687.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(21324..23057)
                     /locus_tag="OQM34_002258"
     CDS             complement(21324..23057)
                     /locus_tag="OQM34_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1452688.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFANMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDLVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(23054..23548)
                     /locus_tag="OQM34_002259"
     CDS             complement(23054..23548)
                     /locus_tag="OQM34_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1452689.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(23674..24024)
                     /locus_tag="OQM34_002260"
     CDS             complement(23674..24024)
                     /locus_tag="OQM34_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1452690.1"
                     /translation="MPPRTPKACRVRGCSHTTTDSSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDCIRARVLKRDKGLCQLCLRAGVVREAKTVDHIIPKARGGTDADSNLQSLC
                     WPCHKAKTAREWLK"
     gene            complement(24476..24868)
                     /locus_tag="OQM34_002261"
     CDS             complement(24476..24868)
                     /locus_tag="OQM34_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCS1452691.1"
                     /translation="MKLSYKLAISAFFFTVIGSFIWSANHYYSKYQHEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRTQADIKVAVADDDCAVRPVP
                     AAAADRLRKFADSLRASSGYTVTGESDR"
     gene            complement(24852..25328)
                     /locus_tag="OQM34_002262"
     CDS             complement(24852..25328)
                     /locus_tag="OQM34_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCS1452692.1"
                     /translation="MQVLNSQRIAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLISRFKEAGGVVNEVEL"
     gene            complement(25332..25658)
                     /locus_tag="OQM34_002263"
     CDS             complement(25332..25658)
                     /locus_tag="OQM34_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCS1452693.1"
                     /translation="MPHNPNTWLELFQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            26020..27097
                     /locus_tag="OQM34_002264"
                     /pseudo
     CDS             26020..27097
                     /locus_tag="OQM34_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015700614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27116..27442
                     /locus_tag="OQM34_002265"
     CDS             27116..27442
                     /locus_tag="OQM34_002265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452694.1"
                     /translation="MSFFKFIIRFLSAFAFGFAIFYFASRKIDLSGISTPWSLITLLA
                     FPFSYCIAAFFKVSEADENTSLSDNELRRLRPIIDAKKRHLGFLIFFYLIAAFSGAIG
                     VMTPTY"
BASE COUNT         6630 a         7469 c         6596 g         6789 t
ORIGIN      
        1 ggtgtttgtc cacaccaaac ctgcctaccg aagcgacgga acaaaaacag cagcggtaag
       61 gaagatgaga accgacagca acaaggcatg gaagggagcg ttaaagcggg caggcattag
      121 caacttccgc ttccatgacc tgaggcatac ctgggcaagc tggctggttc agtccggtgt
      181 ctctcttctt gcgcttaaag agatgggagg atgggaaact ctcgaaatgg ttcaaagata
      241 cgcccacctt tcagccgggc atctcaccga gcacgcgagc aaaatcgatg cgattataag
      301 tcgcaatggc acaaatacgg cacaagagga gaacgtagtt tacttaaatg cgaggtaact
      361 tattgattta aatggtgccg ataataggag tcgaacctac gaccttcgca ttacgaatta
      421 taagaatccg attctaattc aaagcattac cccatcaaca ctgcgctcac acgtcccacc
      481 acatcaaaac atgtaaagcc ttgcaagcca ttgtgaggcc ttatgtgtct cagttttgtc
      541 tcatcagaca tagcaagtat cgatcaattg agacttggat gatagacttc atgcctttca
      601 gagctcattg attaaataaa tgttaaagtt atttgtaaag tacacatcta taggtgtgct
      661 gaacacactt atacactggg tggtttttgg tgtatgtatc tatgccgcac ataccagtca
      721 ggctctggca aacttcacag gtttcgtagt ggctgtgagc tttagcttct tcgcgaatgc
      781 aagattcaca ttcaaagcat cgactacagc gatgcgctac atgttatacg tgggatttat
      841 gggaatattg agtgtgattg ttggttgggc agctgataaa tgctcacttc ctccaatagt
      901 cactcttatc accttctccg ccatcagtct ggtgtgcggt ttcgtctatt caaagttcat
      961 tgtctttagg gatgcgaaat gaaaatatct cttgtcgttc ctgtcttcaa tgaagaagaa
     1021 gcgatacctg ttttctataa aacggtacgt gaattccaag agttgaagcc atatgaagta
     1081 gaaattgtat tcataaatga cggaagtaaa gatgccacag agtcaattat taacgcgctg
     1141 gctgtttcag acccgctagt tgttccgctg tcatttacac gcaactttgg taaagaacca
     1201 gccttatttg cagggttaga ccatgcaagc ggcgatgctg taattcctat tgatgtcgat
     1261 ctgcaggacc cgattgaggt tatccctcat cttattgaaa agtggcaggc aggtgctgat
     1321 atggtactgg ctaagcgctc agaccgttct acagacggaa gactgaagcg catgactgct
     1381 gagtggttct ataaacttca caataaaata agcaatccaa agattgagga gaacgtcggc
     1441 gatttccgac taatgtctcg cggagttgtg gaaaacatta aattattacc agaacgcaac
     1501 ctgttcatga aaggagtatt gagctgggtt ggtgggagta cagatgttgt tgaatatgct
     1561 cgcgctgagc gtgttgctgg cagcactaag tttaatggct ggaagctctg gaacctggcg
     1621 cttgaaggga ttaccagctt ctcaacattt ccgttacgca tgtggacgta cattggcctg
     1681 ttcgttgcag gcctggcttt cctgtacggt gcctggatga tcattgatac gcttgcattc
     1741 gggaatcctg tgcgcggtta tccatcattg ctggtatcaa tactttttct cggtggtgtg
     1801 cagctcattg gtatcggagt tttgggcgag tacattggcc ggatttatac cgaagttaaa
     1861 cagagacctc gctacttgtt aaaagagaaa taaaatggct gaaagaataa ggtatgtatt
     1921 ccttgcaatt ttttgtgcat tactgtgcaa ggagtcaatt gacatatgga tgatgaataa
     1981 tggcgacttc gatagggcca taacaccttt catgaatggg atcagacagt tctcacatga
     2041 tggcacatta ctttataccc tgaaggaaaa tttcagttca attgtgaact atgagtataa
     2101 aagctcattt agttatattc tttatttata cgcttatgtt atatcgatat tcacacacac
     2161 atttgatctt aggttatggt catctctttc caaaatcgtg tatatttgtg cgttatataa
     2221 actatttgta gaaataaaca ataaaaaata ttcttatctt ttatttccgg tatcagcatt
     2281 attgctattt actccttcca tactttcaca attcacagca ttttatcagg agcaaatagt
     2341 aatcacttta cttccggtaa ttacttatat aattcttaaa aaagaaatat caaggtttga
     2401 taaagtatta ctagttgcat gcgcagcgct aatcgccaca tcaaaatctc agttcttcta
     2461 tattcccatt ttaatttctt taatttcaat aatatttatt gacataagag ataaaaaatt
     2521 tcacttagct atggtaattg cgacgataat tgcattgtca ttttcatttt tctcgccaag
     2581 cgcaactaaa tataacagct atcactccct ttatttcggg actttgctat acaataaaag
     2641 caatggaaaa gaaaatccat cgtgggccgt ggacgaatgc attggtgttg atgcatgggg
     2701 aaataaattt gatttggata agggagctgt taccacaaaa gatgctggag cctgcttcgc
     2761 aaaaaataaa gaaaggggat tgaaggattc tttgcttttg atgctaaagc aaccttccat
     2821 gttcctcctt cttccttttg attctggagt tcgaacccaa ttaactgaag attacttcca
     2881 tgtcttcaag gaaaataagc taataataag taagagtgtt tttttgaatg aagttcaaaa
     2941 aataaaagac tctgctctat caactgtgag gattcctgtt gcatttatag ccatgatagc
     3001 atcactgatt tttatccgaa aaaaatattc tgttgcaatc tttacattat ctgcttttag
     3061 catctctcag ttttatatta gcttcattgg cgaaggatat agagatttag acaagcatct
     3121 ttttgcaatg aacttttcat ttgacctaat gatatttata gtactatcaa ttatattgga
     3181 taaggtagca tcaggaattt ataagtaaac atccccccca cagttatcct ggggggggga
     3241 ttttatgtag cgttaaatct tgctctatag tcttttccta atgctatgaa atctatgtca
     3301 gtgggagcgc ctgcagtatt gtacatcctt acaacgaatg agtttgcggt aggataacca
     3361 cctattattc cgtatcctgg tacccctaca gtagttattt ctacagccac gtttgctata
     3421 tcatgatttg catttactgt ataaacccca gtcgctcccc ttgtaacact gagtatattt
     3481 ctcctgtttc ccattgctcc agtcgcacca acaacgctca caccaaaagc gaagtcagga
     3541 gagcttttcc atttaacagt agcatccaca accggtgatt gtgatgggat ccataagtta
     3601 tcataatcga taaaaatagc ctgctcattg gcggaatatc cacttatact aacatctggc
     3661 ctcgatgcgc ttgccgtata gccaacccca aggcaggtat taccgcgcat ctctagtgtc
     3721 atcttaaccc cttccgggta agaataatta ccgcccaata ctacaacctg gctgacgggg
     3781 tactggctgg aattattgtt aaaattagag ttaataatga atcgccacag gtttaacaga
     3841 cacctcagag tcatttaaga tgacttaaag agaggtgccc atgagcggta agcgttatcc
     3901 cgaagagttt aaaattgaag cggtaaaaca ggttgttgat cgcggccatt ctgtttccag
     3961 cgtggcaaca cgtctcggta tcaccaccca cagtctttat gcctggataa aggcatatgg
     4021 cccggattcc tcaaccaata aagtccagtc agacgctcag gctgagatcc gacgcctcca
     4081 gaaagagctg aagcgggtta ccgacgaacg ggacatattg aatctacagc ggcgtacttc
     4141 gcaaagctgt ccgactgagg tacgccttta tccgtgacaa cacccattgc tggcctgtcc
     4201 gactgctttg tcgggtgctg gatgtgcatc cgagtggttt ttacgcctgg cttcagcagc
     4261 cggattcacg gcggcatcat gctgacctga ggctgacggg gctgataaag cagttctggc
     4321 tggagtcggg ttgcgtttat ggttatcgca agatccacct cgacctgcgg gataccggac
     4381 aacagtgcgg agttaaccgt gtctggcgac tgatgaagcg tgccgggata agggctcagg
     4441 tcgggtaccg tagcccacgg gcacgtaagg gtgaaaccag catcgtgacg cccaacaggc
     4501 tccagcggca gttcaactcg gaagcaccgg atgagcgttg ggtaacggac atcacctaca
     4561 tccggactca cgaaggctgg ctgtatctgg ctgtggttgt tgacctgttc tcgcgcaaag
     4621 ttattggctg gtcaatgcag ccccggatga caaaggatat tgtcctgaat gcacttctga
     4681 tggccgtgtg gcgacgtaat cctcaaaagc aggtgctggt tcattctgat caaggcagtc
     4741 agtacacaag ctatgagtgg cagtcgttcc tgaaatcaca cgggctggag ggcagtatga
     4801 gccgtcgcgg taactgtcat gacaacgcag tcgcagaaag ttttttccag ctactgaaac
     4861 gtgaacggat aaagaaaaag atctacggaa cgcgagaaga agctcgcggt gatatttttg
     4921 attacatcga aatgttttat aacagtaagc gtcggcatgg ttcgagtgat catatgccac
     4981 caactgaata cgaaaaccaa tattatcagc ggctcggaag tgtctagatt atctgtggcg
     5041 attcatattt ctgcatcaac tgaatatctt gctgtgggtg tcggtatccc tgcatattcc
     5101 tgtttagatg ctcctggctc atataaagct ggttatgcaa tttgccgttc tgcagatttt
     5161 aactcatggg aatatgtgcc agaccatcgc ggtgaaaacg tttatagcac cgaaacagga
     5221 gaatcgaaag aaatcacagc tctgggtgat taccctgaaa atacaaccac tatcgccccg
     5281 ttaacgccat atgataaatg ggatggtgag aaatgggtga cggatactga ggcacagcat
     5341 agcgccgcag taggtgcagc agaagtacag cgccagtctc tggttgatgc tgctatggct
     5401 tccatcagtc tgattcagct gaaattgcag gccggacgta aactgacgca ggcagaaaca
     5461 acccgcctta acgctgtgct ggattacatt gacgcggtga cggcaacaga taccagcacc
     5521 gcgccggatg tcatctggcc tgaactgccg gaggcgtagg ccattcaata tcgagtgctg
     5581 ttgaagtatt aacacgcatc aatagcacgc gatatttttt ccatgccagt aacagtttcg
     5641 tttcatcatc tgttgccata cctaaatcaa ccgcatcctg aagcggcgct attacattcg
     5701 ttgcctctgc tatcagtgca gatttctttt catcatttat taaggctaat tgttctggtg
     5761 ttggagaagg acggtcaaca atgacaggat gaccgttctc accacaggta atcatttttg
     5821 ttacactctg tgagttaatc agtgttttcc actctgattc cgagatttcc actgcatcat
     5881 caggaatatt ggtgccgtga aattcagtcg aataaaatcc gtttgtcgat gcagaataaa
     5941 aatatgacat ttcgttaata tcccattgca atgtaatacg catcagaaga tgcaccatat
     6001 cctgaaccgg gcgacacagt aatattaaat ccagtgttat ggacatttgt cgccgataca
     6061 gtcggctgcc cagccgcgcc agtaacgttc aatgtaactg tgacagcaag gactcttgag
     6121 aatgtcatcg ggaacctcat cccgtatgtt gaagatacat taggggagcc attcaactga
     6181 ccgcactgta aaattaatcc agacggaaat ttttgcgcag cagccgttgc ggtattactt
     6241 gacgcgaaca ggctcatatc aggtatctga ttcgcccctg tacctacatt ccttttagct
     6301 gcttctccca aaccaaggtt ttcgagagcc gttttcaccg tgccatccga tttgatatcg
     6361 ccaaacggat tcttgcggct taacagcagc gcgcgaagtg cggtaagcaa ctgatcgtgc
     6421 cgccccttct ccaggctggc accggatgcc tccaccacgc tgcaaagctc ctcctgcaac
     6481 atgtcaaagt agtcatcatc cagatcggtg gcaggcgtgc cagtctgggg gttaccacgg
     6541 gtaaaaccgt tcttacccgc gccgaactta tccttctgcg cggttttcgt gtctatacga
     6601 tgcatggatt actccggata tttaaaaatc acataggtat gcgacgggca gagtttgtta
     6661 agcacacact cgacaacggt gtccccccag aagcgcagcg cggaatcaca gggatcgcca
     6721 catgtcatcc aggtggtgtt ggtggcgact ggcatgttga cctgccagta ataccgccat
     6781 tccggcgcat tcaccgcgtc agtacaggca gatgagcagg tgaacgtgct tttgtcgtat
     6841 cgcgtgatgg tagcgtctgg tctgcccagg gcagcaagct gtgcaaggta aaaatcctca
     6901 ttgatgccgc ccgccaggtt aaccttcgca tccagtcgtt gctgacgctg gcgaagggtc
     6961 tgtgtccctg cgggaataca ttcatccggc agaccgcaca gacgctccca gcggtttatc
     7021 agttcagtgg tggtgtgcgg atccagctcc cgcatcaggg catccgcacg ctgatgaacg
     7081 cgggttaatg acggtgccgc accggcaatc gccggatctc tggctgacca cgccggaccg
     7141 ggtggcaaca gtgccgacaa cagacggatg taatcatcgt ttgtcacgtc catgaaatcg
     7201 tccccagtac cgccagttca ttttttgcaa tggagatatt gtccgccggt gcaagcaact
     7261 gatggctgta ttccccgttc gcaccggaaa tcgcttcact gatacgtgac accttcagtt
     7321 ctccctgcgg ataaccatca cgcagcagga acgaacgcaa ctcggcggta atggcagccc
     7381 gtatttccgg tgtgtccggc gtcacacgga tatgaaaatc cactttatgc gccaccggcc
     7441 tgaatacata caaatcagag cctgccaccg gggccagtgg cccgatatgt tgtcttgccg
     7501 ccgtttccgt tgattcttcc ggaatgggat taatcaggtc actgccggca atcatcacac
     7561 cgacagtccc cgttcccatc cagtgtcggt atgtccatgc gcgggtaatg ccgggcactt
     7621 ctttagccca gacgacatag tccccgtcag ccccgccctg aggcgtccag taataccgct
     7681 caatgacgcg ggcgcgccac gtttccagct cttcagtatc aaatccacct gtcagggtat
     7741 ctgccacgcc ggaagacggc agaccattca ccggcgtgac caggattaat gacgtaccgt
     7801 cgtcagcgtt accgaccgcg cctgcacttg agcaggcgat cggcacgcgc aggacaccac
     7861 cggtgctggt tgcatcggca gttgccgtgt actgcaccag gtcatcgcgc tgaataacac
     7921 tcccggcggt caccttcagg ccatcgctga caccttccca gcgcatatac ccgctggcag
     7981 ccgtggcccc cttgcgcgga caccgtttca tcgcagcatg tcgcgccagc caggactcat
     8041 cgcacaggtc aggcagcatg ttcattgcca gataatcgat gtaaccgtaa accgtatgca
     8101 gcgccgccgc atacaccttt gcccgcacgt cttcatccat gcgccggagc gtgtcgctga
     8161 cgtccagcct ggcgaataaa tcgttacgga gcatactgat attttctgcc agcgtcgggc
     8221 gctgaaattc actgtccgcc atgcgttatc gcactccaca gatcatcaaa agaaatcatt
     8281 actggtccat cacgacgcca gagggtgata ctgttaccca gctcattaat cccggtgcgg
     8341 cggatatcca gatcaatacg ggacaccacg ccgtcatcaa tcatccattg caggcattcg
     8401 cggatatatc cccttaccgt ctgcaccagc tgattggtca gtttgctgcg ctgaagcagc
     8461 cacagtcggg agccgtaacg gtcattctgt accgcaggcc aggtatcccc ccaccatccc
     8521 atcgggacgt cggcgttgtc atcaggctcc gcccgccgcc aggtaaacag ggaaatcacc
     8581 acggcgcggg tcagcggatc cagcggtgcg ctggcgcagg tgcgtttacc gttcaccgtc
     8641 agccacagtt ccatcatgcc tccatcgctt tatcaggttt gtcggtgtta ctgccctgac
     8701 cgttctctct gtgacgatgc ccgttatagg caagccgcat cgctgacatg gtggtgccgc
     8761 cggagtcgca caggtctttc acctgtcctg tcacttccag gtccatttca aaacgtgcct
     8821 taggcgcatt gcgaaacgtg atcgttttac ctgcaccgtc caccacgatc ccctcccggg
     8881 tcagcgtcac agactgcccc tgatcgtcat agacagccac ctcacccgtc tgcagccctt
     8941 tcaggcggta gcgccggtcc gacaccgtaa caaccaccgc atgagaacgg tcgccatccg
     9001 gaaacaacac caccgcttcc gcaccgctgt ttgcccttgc ggtaaaaccg tagggttcaa
     9061 gatgttcaat cccggctttg ggttcaccgg caatcaggga cacatccacg gtctgacatt
     9121 tcgtggcggc actgatgctt ttcaccactg cccgcccaat caggccgagg agttgtcgct
     9181 gcatggcttc aatcgtcctc atcagaacgg gtcctcctgt actctggctt ttttcttttt
     9241 ccgcgcgccg ggggcttcgg gttcaggcag ataagcatca ggcgggccga cacggatttc
     9301 cgtcagggtg ccgttctggt cctgagtaaa cgtgacttcc gaaacaagca gttcggtatt
     9361 gtcgaaacca cagaccggat caaagacaat cacccgctgg ttgggctgcc acagcgtacc
     9421 gttaccctgt cgccagccct gcaccacata ggtggtttca tccgtccgcg ccgcccgttg
     9481 ccgggcttca aagtcagcac gcgcaataca gcctgccccc gtagcctgcc ctgtctgcct
     9541 gatatacatc ggacggtaac gggcaataaa tgcgtcctct gtgcgggccc gcagcgcggt
     9601 ggtggtggcc tcaccgaaat catcgtcgtt tccggcacgc tgccccgcca cctggtaaac
     9661 agaaaatcgc tcccggatac tcttctccgt atcgcaggaa aggatgtttt ccccgagtac
     9721 cagcgcagta tgtgcccgcg ttgagccaat accgccaatc accagcctgc cgtgcgggtc
     9781 gtcgtaagcc agtgcctgct gctgaccgag tattttgttg attacctcaa tcaccgtttc
     9841 accgtgatca ggctgaacat caggaataac acccgacggc gcaccgctgt tcaccacctc
     9901 aatgccgaaa ggcgcagcaa gcgcctgcgc aatctgtacc agcgatcgtc cgttaaactg
     9961 tgtcggttcg gctgcacagt caatcaggtc agcggtcaga ctgcgtccgg caataccggt
    10021 gctgaccgaa cgggcatcgt aacgaacggg cgtcgcctcc acccagccgg tgatcaccag
    10081 ctcatcacca atcagcacct ccacttttga accgttttta atgcgcggct gaagcgtggt
    10141 aataccctca tctcccggcc actggcgggt gatctccaca ctgaaatccc gcgccagccg
    10201 ttcaataccg gcaccgatgc gcaccgatgt ccagccattc cactcccggc catttacccg
    10261 tagcgtgaca ttgtcgttca ttgcactggc accttcagag ggatcaccgg cacaaagccg
    10321 ggatgcgtaa tggcattacg ccggataatg tccgcgtcac gcgccgcgtt atcaaactag
    10381 gtcgccgcca gcaccagcgc gggtaaaacc tcatccggtg tgcgctgaat gatccgtgca
    10441 gactgttcaa ggcgcgtgtt gatatccgca ttcagatctg ctttcacccg gcgcagcgcc
    10501 agaaacagcg catcgctggt tgtacgggac aactccttat caattgccgt attcagtgtg
    10561 tcgcgaatgt cagtcagttc ttcccacgtc ggcaggtcaa ccgtgttttt caccgccggt
    10621 gcattgttca gtgccggatg cgtgacggaa ggccagcaag tgctctgcgc gggtgttgtt
    10681 gcctgcccca ctgcggcatt ctgcatcacc gcggaagttg ttggcgcagg caatcgggtg
    10741 acggcatacg ccgcttcgct gattgcggtc gtacgaaggg tgctggcaac cacgttacgc
    10801 tgctgcgtcg ccgtggcggt ggttttactg tccgttttcc agacgccgcg gggttgcaga
    10861 tcgctgccga ggctgacacc ggaaagcgtt ttgatcatgg tgaccaggtc gctggcgtta
    10921 ccataaaggc gtttcccggt acgccacatt ttctgcacct gctcaacgaa atttttgcct
    10981 gacgatggcg gcggcagaag taccgagata tccccctgca acagcctggc ggcatccgat
    11041 acggcagaat ccaccacttt catcgcatca gaaacatacc ccagcattat gctggcatta
    11101 ccgataacgt cgttctgcac gaaatccgcc acaccatcga tactgaaacc gctgaagctg
    11161 tcactgatgc agtcatccag tgcagaacag gatgacatca gcgtctgcgc cgtcgccgca
    11221 cctgatgtgg ggtaagagag ttctcccgct tcgacaaact tcaggtcaaa gcggacaata
    11281 cgcccttcac tcttcgatgt gctgacccga acttctccgt caacacagac tttcagctca
    11341 ccgtatgtcg gatggacaag cgtgccggga ccgggtttat tcagcgcgtc aatcaggcga
    11401 tcgcgctggt caaagcagtc atctcccacc acataagccg tgatggacgg gcgaaaagtg
    11461 attttcccca ggtcttcggt atagggtttg tcgcggttcg ggtattcatg tgtttccaca
    11521 cggcgaccgg ttcccgcact ttcttcttca accttaaacg gcacgccgcg aaatgacgcg
    11581 tcctgaagtc tgtcacgcca gcctgaagac gacgaaagta atgaaggtcg ggtgggaaat
    11641 gaggataaat ccatagactg acctcaaaaa ggactgcgtt atcgtggaaa acgaaaaggg
    11701 gaatacccca catcgtgcgt gattttcatc aggggatcgg ctttgcccgg tacatcaatt
    11761 atcttcatac ctagcggagc attctcgaac gtgactttca gctcgctgtg ctgtgtcatg
    11821 gaagaagatg gattcaacag cggaacattg ggtttgtact ggctcaggct ggcctgatac
    11881 tgctcgtact ctttacgatc aaaaaaaggc gtccagtctg aagccagaaa cagcccttta
    11941 ttatccagcc agttaaccgt atcttcagga acaacacttt ccagagtatc tttaaccggc
    12001 tcatacatca gggttcccaa aaaaccatat accccggcct tcccgataaa gccgcggcct
    12061 ttccccatca atcccgtttc tgccgatacc ttccccagcg tacgcatctc tctggtcact
    12121 gcggtaatgg atttggtaac gtcagcaacc catttggttg ccataaacag ggcaatcgct
    12181 ttcagaacag tttcccatcc ccccatcacc tgcgccgttt catccaccac gtgccagact
    12241 ttttttatga cagggcctac ggtttcccaa ttatcaataa tgaggtaagc gccaccaacc
    12301 agaagagcaa tcagcccctt agcaggcgtc atattcatca caccgccgag aactttcatg
    12361 attctggaca aagagcctgc agcggccccc actgtcagta aagccagacc gattttagca
    12421 atggtcttaa cgagctccgg gttttcacgg acaaacgttc tcacttcctc aaggagcggt
    12481 tttaccgctt caagaccatc attaacctca ggaagaaacg tttcccccag cgtggaagaa
    12541 atggcatcaa gttgattttg cagaagtaaa agctggtttt ccgtcgtcgc tgccctcgaa
    12601 gcatattcct tctgcatcga actgccatac tgctgggaat ccgcaacccg cctgaagttg
    12661 gtacgcaaca aatcaaggtt agtcagcaga ggtgctatcg cgcccagaga ctctttcccg
    12721 aacagggcat tcagcacagc tgcctgtttt tctttaggca ctttagccat cgcatccagt
    12781 acagacagca tggtgccccg ggcatctttc tgcatatcag cagctaattt cttcggattg
    12841 atccgcagaa aacgcaatgc ctgtttctgc gattttgtcg cagaatttcc cgcggtcagg
    12901 gaaagcatga agttcttgac ccctgtggcg gcaatttctg actccacgcc catcccggca
    12961 atggttgccc ccattgccgc gatttcgccg gaagccacac ctgcaacacc acctaaagga
    13021 ccaatacgcg taacaatatc ggagattttc ttcgcgttcg ctgggccggt attaccaagg
    13081 tagttgattt tgtcagccag cccggccact tcatcctgcg tcatattaaa cgcagtacgc
    13141 cactgggcca tcatctgccc ggactcttca gccgtggtat caaaggccac gcccatcttc
    13201 accgcatcag tggcaaactg catcagttca tcacgtgcaa tcccggcctg accgccagcc
    13261 gccacaattt ccgcgatccc gtctgcagac atgggaagct cagtagacaa agcgcgtacc
    13321 tgctccgtca tggccttaaa cgcatccggc gtatccagac cgtccaccac tttgcggaca
    13381 tcagccatct tcgattcaag ggtgatggct gattttacag ggagtgccag tgcccccatt
    13441 attgcagtac ccgccccggc agcgcccaga gcaaggctgg agacttcttt ctgaaatccc
    13501 ttaagctgac gctgcatacc tttaagcggg ccggacagcc tgtcaacagc ggtgatgatg
    13561 gctttcagct gaaaattatc agccatgctt catctcctca tttatacgga cagcctctgc
    13621 ctccagatca gcaaagtggg aaatagccgt ccggcgaagt tcaagggggt ttaatttcca
    13681 gaaccacgcg acattgtaga atcgcttccg gagctctctt ccgtctccaa gccggtaaaa
    13741 aaacgcatta caatcatgcc tgccttgaaa atatccagct tcgtcatctg cgctgcagac
    13801 gagcgcggga tcccggccag aagcgggata tatttcagcg ccacctgact gtccattttc
    13861 ataccaccat caggcgaaac agagaaaggg aaccccagcg cctcaatctc gtcatacgta
    13921 ggctcacgta tttccagcac atgcagtgtt tctttgtggg cgatgatcgg ttttttaagt
    13981 acaagctcaa tcactggtaa tccccttctt caccgtggaa ctcaagatca accgtgcctt
    14041 cttcggcatt atggttcgct tcgccgtgca gccaggcaga cgacaataca tagacctgac
    14101 cgttcgccag ctcggcagtg atggtcatct catcagacga ggtgattttg ctcaccggaa
    14161 aattcttcgg caccttgaag gtccctttga cataaggcgc acggtgagtt tccttgcggt
    14221 ccactgaacc gtccaggccg atgatgtcat cattgaccgt cctgttcatg ggcacctcaa
    14281 tgccgccggt cagcgatagc tgctgaccgt caattttgaa ataacaggtt cccccgatac
    14341 gggccattat gcagactcct ctgaatactg aagacggagc tggttaacca cggcaaagac
    14401 acgcaactgg ttaacatagt caggcgggaa cagcgtgttc aggcggttcg gatcgctggc
    14461 atcacgctcc aaaaccaggt actgcttaaa cagttcgtag ttttccacga tccccgctcg
    14521 ctcaagctga cggtaggttg ccagcagttc ccctttgatc accgccgggg tgacaatcgc
    14581 ctgaccggga ccaaagcggg taccgtcact ggcaagcttg tgacgcccgt acttactggt
    14641 aatgacggat ttcagtttgc gcagtacata cgcgctggta tgcagcgtct cgctgtcgag
    14701 gtagctgtta tccgcaaccc cgtaagcgtt tttcctgtac gtggtgacat cacgctgaat
    14761 gcgcagtacc ccgctttcga catacgccgt tgccacgcca tgagacagca gggtctgttg
    14821 ttcggtcatc gtgaaccgtt tccccttcgg cgcaggcagc atacccacca gctcaccggt
    14881 ctgcgtggga cgtgccggat cgttgcgaat aaacaccgct gcgcgggcgg tacggcttgc
    14941 cgccagctca tcggcaggcg tctgggtgtc tttttcgtac cccgccaggg taatgtgctg
    15001 ctggttaaac tggtcacctg cggtcaccag ttctgacagc gtgccgatct ttgccgtata
    15061 cacatgacca tacagctgac gcgcatagct ccagcgaccg ctggtatcgt tcatctcggt
    15121 caccagcgtg ttaacggagg ccgtgtcgtt gaacggcagg ccgatataat caaacggctc
    15181 atccgccatt gcagccaccg cgccggtgag aaccggagca cccgttccgg cggtacccgt
    15241 cgccacggca atctgtacgc ccgctggcag cacttcgccc ccaccaaagc cgtagtaatt
    15301 gaggctgaca ggaatttcat tcccgcaaag ccccttatga cgcgcggtca gtgtgaccac
    15361 gcctgccgaa gatgaagccg taaacggcag ggccggaacg gcattgatgg catctttgat
    15421 actgctggca atcgtcgtga cgttatcgcc gttggtcacc ggagcctgca cgcgggtacg
    15481 tcccacatag acattcaccg tgccgctttc ggttgcttcc ccggtcaccg tcagcgtaac
    15541 cgttgccgcc gcgcctgtgg cttccggaac ggcaatcaca tacagctcac caaacgggtc
    15601 ggtctggcga taagcctcga ccatacgcgc cagctgactt cccgtaccac aaatctggcg
    15661 tgcatagtct gccgacggca tcagcaccag actgttggca acaatctctg caccgttatt
    15721 ggcatgacca atcagcagcg atgctccgct gtcctgtgca gtattcgcag cctggttatc
    15781 catttccgca taaaacagcg gaaccagcgt attcgacgga atggtgttaa agcttatcgt
    15841 catcggtgtt caccttttta ttcacgcgcc ggatatcacc cgctgcttca cggcgcagcc
    15901 agtagttgtt ctcgtcaaca tttcgccctt cggcgggcaa aaggtcgccg cgggcagggt
    15961 caggaactga ccgcccttta acaggtttga caaacatgat gatcctcagg aaggaagggg
    16021 tatttcggtg tgatgttcga tatcgccgtc aggcccgtta ccgggatcga gataatcaac
    16081 atcaatcgcc agcgttcgca gttcatccag actgttcagg tcatcctgct ggcgggtatc
    16141 gtcttcggtc agctcgctga tgaccgaaaa atcgaactga taaatcagct catgacgatt
    16201 cagatccagc agcgtgccgc cgtcataggt aatcgggtta ccgcacgctt ccgggttcca
    16261 gcccagcaga gccttaaaga gcatctgccg gacatcgtcc accacatcat acgaggcaaa
    16321 ctgaccgcgc tcatcacgcc cgttactcag tatgacaacc acggagaagc cctctttcag
    16381 ctcctgccag tagtcggtct gacttttgtt ttctcccgga gagtcatcac ccggtaccac
    16441 atatgccgcc gggagtttca actttccgac ctccggcaga tttttgaact gggccgcgcc
    16501 tgccacccgg ttttcaaaat acagacagcg ggcacgcagc gcagcaataa caggagtcag
    16561 tttcatgttt ttttccttct tacaggacgt aatgaccgct gtaactcacg ggacagtaac
    16621 ttttgtgtcc agtaacgacg ctggtcgatg acatcagcca taaagttatt acgtggtgcc
    16681 agccgccagc gggatgaatg ctgctttttc tggcgcttat cctttttgct catcccgtat
    16741 gcggcatgac gcacaccgta atacagaaac gccggataat acgcagaacc ttcaggaaaa
    16801 cgccggttac cctgcccgtt tttctggtta ggggaaatcc tgaccatcag gccagaacga
    16861 cgggagcttt tacgggggac gtaataacct atagaacgcg caagtcgtcc ggtctgatag
    16921 ccggggtttt cacctggagc agaccgacca cgtcgcatca ccagccgccg ggcatcacgc
    16981 atatagacac gcccgatttg aacaaatgcc cttcggagcc tggcacggtt aaactccatc
    17041 tcctccggtt gtttgaaatc aacgtgtaaa aatgctgtct gatccactgc gtcctcccat
    17101 ttgttcttcg gcacccagtt ccgtacactc cagcagcaga aagcgccgcg ccccgttcag
    17161 atcacgctga cgtttcaccc ggtacacacg gtcatcacag accacctcat aatcagcagt
    17221 gatcccccgg cggtagcgaa tggtgatgta atgggtgatg gcgtccccgg tctgcgcggt
    17281 ttcctgccag gtggtggcac tggtctggat aaccttcgcc catgtccgga acgtaaccgg
    17341 gtattgaggc tccacgccaa agttatccgc gggcatatcc acccgctggc ggatcaggac
    17401 gcgtttattc agttcaccgg ggtccggcag aatgtaggtt gcgctggtct gcgcctgacg
    17461 aattttcatt gcggaaagta cctgtacggg ccgacaagcc agccaaaact ctgcggcatg
    17521 tcgagtttct ccacttccgt aaccgacgag cggttttcgt aaaaatggct gataagcatc
    17581 agcatcccca gacgaatatc atccggcagg tgcagcccgt ccggatcgct gtccggaatg
    17641 gtttcatccg gtgcatagag cttccggttc agatacgttt ccgtccgctt ttgtgccgca
    17701 caggccagca gttgcagatg gcggtcatca acatcgaaat cctcatccag ccggagttgg
    17761 gctttaatct cttccattgt cagaagcata ctcagccctc tttactggtc gtggcttttt
    17821 tctcttttgt cgctttactg ctttttgcac tggttccgcg ctctgctaac ccggcctgaa
    17881 gtgcaatctc ctgcacccgg gcaggaagcg ccccgtcgtc atactcaccg gcccgaatga
    17941 cctcaacacg cataccgtcc ggtgaccatt tcagatcttg tttcaggatc atgattcttc
    18001 acccgtcaga acaggggcgc ggttccgcgc ccctgagtga ttacgccact gcaatcttca
    18061 gcagtttgat ggcctgcgaa tcgaccagca tgccgccggt gcgtttggtg gtataaaaac
    18121 cgacaaacgg tttattggtg tacgggtcac gcagaatgcg ggtgccgata cggtcaacga
    18181 tggtgtaacc ccgtttgaag ttaccaaatg caatggcttt cgcatcagcg gcgatatccg
    18241 gcatctgttc gttttcagcg ataccgtaac ccgccagaga ggacggctgc cccagctcca
    18301 gccccggacg ccacagatag ttaccctcgc tgtctttcag cagacggatg gcaaacaggc
    18361 tattgttgtt catcatgaac ttcgcgcctg tgcggtgtgc cttacgcagc gtgtaaatca
    18421 gtttgataat ggcgtctgcg gtcaccgccg tcgcgtcgcc ggatacaata tgctgaagtt
    18481 tgccgaacgc ccggacctta tcggtttcat ccgtggattc atacgccagg aaccctttcg
    18541 gcttcttggt accatcgccg gtggtaaagg caatttcttc ctgttcggca aattcggttg
    18601 ccagctcgct gttgatccat gcttccacgt tgaaaaaggc atcatccagc attttctggg
    18661 tggcctgcgg gttaccgtag atttccccca tgaaaggttc aatcaggccc agttttgagg
    18721 tggcagtctg ggagcgcgcg tcagtctcgc caacccatcc ggaagccgtg ccgcccagat
    18781 tcaccagttt tttgtagtcg gaaccaccaa cggtgatcac cgtggcttcc tggcgcatca
    18841 ccacttcatc tttcagcagg gtgagaatgt tgcgatccag tgcttccggc acggcatagc
    18901 cgccgtcttc atcggtgccc acctgtaatg ccttgcgctc cagatcgcgc agaccatctt
    18961 cacggccttt acgcaggaag cccacaaacg cttctttatg ctcggtggcc agtttatttt
    19021 gcgcaccacc tgccggacgt ttcagctcaa gcagctcttt ttcaagatcg cttttgaggt
    19081 tttccagctc gctgagtttc ccgttcaggg tttccacctg cccggcaagc ttgccttttt
    19141 cctgctcaat cgcatccacg cgcttgtcgt tctttgcttt gaagtcgtca aacttctgct
    19201 gcagctcctg cgcgacctgt tcgacatctt taatatcaac cgccatcata tttctcctga
    19261 ttagaagttc agatttttca gtgcattcag tgcagagccc acatcctcag cgtcgcgcag
    19321 ggacagtgcg ccatagcccc cggccatgaa tgctttggcc tgggtacggg agagtccgac
    19381 atcacgcagg actctttcga tttttttctg ttcggggatt tccccgcggg ccagtgcgtt
    19441 cttgacgtcg ctgatccgcg cctcgtcgtt agacgggaac gtcaccaggc tgacttccca
    19501 gaggtcgatt tctttcagca gaaaggcttc tttgctccgg tcgtattccc agtctttcag
    19561 gacgtaccca atagaaaggc cggttaacga accggccttc atgtgtgcat gtgcgcgttt
    19621 tgcgagggga tcatcatcaa taagcaaccg tcccctgacg taaagcccga catcgtcttc
    19681 cttcatttcg gtgtaaacac cgatgggttc atccatgcgg tgctgccaga gcagcgcagg
    19741 taacgctttt ctgtcactcc acgcccgcag ggaagcagca aatgccccgg acatcaccac
    19801 atcatcgtgg ctgtccttta caccaaagac ggagccatac ccttcaaact caccggagtc
    19861 actgacagat ttcagactca gcggtacatc aagacgttgt ttcgtctgca ttggcgttat
    19921 ccttctgctt accggcttta ctgccatcgg agggtttcgt ggtcatgttc atcggtgtga
    19981 gatagacatc accaccggga cgcggattca tatcttccag gtcgcggcag tcattgggag
    20041 agtaaattcc ccagttgatc ccggtggcgt aggcttcaaa acgggacttc atatccccgc
    20101 gcagtaacgc cccggcgtta aatttggcgt aataaacgcc ctgcttactt tttcgtacca
    20161 gtccggtgtt gatccgctgt tcgatgcggg tcagatacgg caccagtgaa tagttgataa
    20221 atcccagccc cagctcttcg atattgttga aggtggcgcg atcggtgttc tgcaccatgt
    20281 gcaacggcac ccggaacaga cgacagattt cttcaagctg aaacttgcgg gtttccagga
    20341 actggctgtc ctcggcgttc agcgccatcg acttccagtc cagccccatc tcaaggatca
    20401 tcgggcggtg agcattgcca agcccggtgt gacgctcctc aaaatctttc ttcaggcgct
    20461 cataagcctg atctgacagc gtctgctctg tacgcaacac acccgacgtc accgcgccat
    20521 tgctgaacag tctggccccg tgctcttcgg tcgctgccgc cagcgatatt gcctcgcggg
    20581 cataggcgat gggattcagc cccaccagtc cgtccagcgt cagcgtgcgc acatgccaga
    20641 tatcctcctg gctcagtaca tccgtggagc catccgggaa tgtgacctga tagaccggct
    20701 cccagctact gttaagcttc ggtaccacac agccgggatc gacgggcagc agttcagcca
    20761 cttcgccaaa tgctttcact ttgtaggcgt aaaagtttcc ccgcaggcac agacaggtga
    20821 ccaccagctc ccagaactcc tgcggcgtca tatagccatt gggatgcgtg gagatcagct
    20881 tatgcagacg ttcgccagcg gctctctgct tcagactgcc gttcaggtga tacaggttgc
    20941 agggcaacat cccgaccgac tccgccagca ccctgacaca ggaaaaaacc gccgtcagtc
    21001 gcatggcccg ctggctgctg atctgctttc cggtataggt gtcgtaggac aacccgatag
    21061 catccgccag ctctgctggc gtggtcaccg gtgcgtcact ttttcgttga aataatcccg
    21121 aaaagaacac tatttacctc cgccgacaga cgactgtgta cggtcgagat atcgcgccac
    21181 cagccacgac cagaacagac acaacgcccc ggcaacaaca aaccccgccg ggggataaat
    21241 cagccaggca ccatacgcca gcaaaagcgc ccccagcacg cccaccagag gcgcgagaat
    21301 cagcatgatc ataattacct cagttaaagc gagcggatcc cataggactc aatgtggtca
    21361 gacagcgtgt cttctttctc gtacagcatg gctctgccaa ccgccataat cagcgcaact
    21421 gcaccatcga ttttgttttc cgcctgctct ttgacgggct tcactaaatc atcgttacct
    21481 ggcatgtttt tgccgaccac attgccgata caccaggtca tgatgggatt gccgtcatga
    21541 tgaaagcgtc ccgattcaat cgctgcctcc agctctttca tcgggtcgga catattggcg
    21601 aagttctgga cgatagtaac gggattcagg tcttcatcag caaggtcatg tgacagcccg
    21661 gtcgctccga aagggtcgat gggtgactca ctgaccgggc tgattttgtt cgccgctttg
    21721 gcctcttcga ggatgtagcg ataatccacc tctgcaccat cggtaacggt cagaacgccc
    21781 atttccaccc atttctgaaa gcgttcggct gtccggcgat cttcattttt ctcgacgcta
    21841 tacaccgtgt catacggtac ccagaaacgc ggggccacac tgtagtaatg cgttttaccg
    21901 tcaatctcgc gggtataaag tcgcgccatg ctgttcatat ccagcttacg cgccaggtca
    21961 aaggccagaa tgcacggctg cccctcgaac tgctcaagag tcagtgattt atcctcgcag
    22021 ctctgccagc tcaccaggtt gaaatacgcc gaacgcgccg acacccagat attgaggtgt
    22081 tttgttttaa agacgtttgc cagacgggcg ttatttttcg cacgctgctg ctgacttaac
    22141 aaaaattcgc gataaaccga cacgccaata tttggattgg ctttttccag cacctgcggg
    22201 tcggtccagt cgtcaccttc atcaacggta tagatgatcc cgaacagttc atcgttaggc
    22261 accgagccgt tgagcatctc gatgacttcc cgccgtttgt cgtagcacgg cccctcaatg
    22321 ttgtacccgg cggtggtgat ggcccacatc agtggctgac gtcgcgcccc catcccggta
    22381 agcattgtgg tataaagcgc atcggtggca tgctcgtgat attcatcaac cacggcacag
    22441 tggggtgatg aaccatcacc tgggttgccg atcagcggtt caaaccgcgc gccatcctcc
    22501 ggacggttca tgtttgaggc gttaacctca atcccgaacg cttccgtcag catgggtgtg
    22561 cgtttacaca tcagtcgcgc cgggcgaaag acttcccacg cctgtttctc tgtcgtggca
    22621 ccggaataca cttccgcgcc aaactcgtta tcacaggcaa aacaatacag ggcaacaccg
    22681 gcagagattg ctgatttgcc gttcttacgg gggatttcgg tgtacacctc ccggaagcgg
    22741 cgcaaccggg tgcctttatt gacccagcca aacgcacagc agatcacaaa tagctgccac
    22801 ggctccagcg tgatgggcat ccgtttgaat gcccactccc ccttggtgtg cggcaacagc
    22861 tgaataaatt tcgcggcccg ttcagccagg tccttgtcga agcggtaacg aaacgactta
    22921 cttttttccg ccatcaggtc atcaagatgg cgctggcagg cctgaatcac aaactggcag
    22981 gcaacaatct ttccgcgcac gacatcccgg gcatactgat ttgcagcatt tacgttgggg
    23041 taagatttcc ggctcatgat tcgataattt tcagaaacgg gttagtggct ttcttctgcc
    23101 ccgccaggcc aatcagacgc tggcggctgc tggggtcgag tccgagcatt gcccccgtac
    23161 tgctcatctc ggactcctgt tcttttttgg cggtcagctc cggatttttg accatgccgc
    23221 ccattgcacc ggtgatggtg ttgccctgtc tggcaatatt tttcacggca cgtcgccaga
    23281 actcataggc cacgcaccac cgctcaagca ctgcgaggtc agtcacgcac agcaggccct
    23341 gaccgcagag ttctttggtt gtcagttgcc acatgatcgt ggcgagaggg agctcttctt
    23401 cagcgaacca ctccggtggc tcaacacctt tgatgggcgt aaaaacgggt tcatctttgt
    23461 tcagggctcg cttgccgggg tttccggcca gcgccttgcg cgccgttggc ttggggcgac
    23521 gcccggaacg ccccgccgtt ccagccatat gcggcactcc tggttaaatt tcatttttcg
    23581 cgggtataaa aaaacgatgg ggcgggcagt ccggaagacg tcaggtcaca gggatttgac
    23641 ccgcccctcc cctctggcag tgggaactgg ttcttacttc agccattcac gggccgtctt
    23701 cgccttatga cacggccaac acaggctctg tagattactg tcggcatcag tgccgccacg
    23761 cgctttaggg atgatgtggt caacggtttt cgcctcacgc accacaccag cacgcagaca
    23821 taactgacac aggcctttgt cacgcttcag gacacgcgcg cggatacagt cccacttcga
    23881 accgtagccg cgctgatgac gggactggcc tggcttgtat tgcttccagc cttcgctttt
    23941 gtggctttcg caatagcctg acgagtcagt ggtggtatgg gagcagccgc gaacacggca
    24001 ggcttttggg gttcgtgggg gcatatgtac tccaatgaag aaaccagcga aatagctgcc
    24061 ttcattcgta gtaaaccttt ttcatcaacg cagtaatgga ttctttgaag agtcgcaatc
    24121 aatacgactc actaatgggg aggcatgtcc aacgcgttgg acgagttgcc tacttgattt
    24181 aggtgacact ttagaaggac agaatgcctt cctcactcaa ataacatcaa ttaaggaggt
    24241 tcaacatgct tcgttccaaa agtcagttga ctgtaattat tactacgtca ccttatgccg
    24301 aagacctttt ccgcttcact ttgagcctga ttcacttcta cctgaccggc tcgcctctat
    24361 ccttctgatc cccgctttat ccagattgca ctgccctagc gccgacaaca gactgacatt
    24421 caaatctagg ctacccgcat aagtcagcgg ttcagaaatg gctggttgcg gggtttcagc
    24481 ggtcagactc gccggtaacg gtatagccgg aactggcacg taaactgtcc gcgaactttc
    24541 gcaaccggtc agcagcggca gcaggcacag ggcgtacagc acaatcatca tccgcaacag
    24601 ccactttgat atcagcctgg gttctctgtg actccagtgc gatctgttgt tttgcatgct
    24661 ggttagcctc cagaactgta ttgacgattt gtagtgattg caggacgtta ttggtaatgg
    24721 ctgttgctga ttcagcattt cgtacagcct catcagcacg tttcttttcg tgctgatatt
    24781 tgctgtagta gtggttagca gaccagatga aagaaccgat gacagtaaag aagaatgcag
    24841 agatagccag cttatagctc aacttcattt accaccccac cagcctcttt aaaccgggaa
    24901 atcaggtcac cgattttatg ttcatactga ccgtaacctg caccaggtaa cgacgcccag
    24961 atattgctgc aacgatcgat agcctgacga atatcgccgc gatcaatcat cggtaaagcg
    25021 ccacgctctt taatctgctg caatgccaca gcgtcctggc ttttcggaga gaagtctttc
    25081 aggccaagct gcttgcgata ggcatcccac caacgggaaa gaagctggta acgtccggcg
    25141 gctgttgatt tgagttttgg gtttagcgtg acaagtttgc gagggtgatc agagtaatca
    25201 gtaaacagtt cgccgccaac aataacatca taaccgtggt tacgtgtcgg ttgtcgcccg
    25261 ttatccgttc cttctgacca tgccaccata tcgaggaaag ctatacgctg ggaatttagt
    25321 acctgcataa attactcctt agagccacca aacttattac cgattactcg cattgcagcc
    25381 ccacgaatag catcaacacc gatcagcccc acaccaccac caatggcaac agatagtgat
    25441 ttaggccatc cgacatactc aagagcggat gcaaaagtca gcgtcagagc gccacagagt
    25501 agaatttcga gtgtttttcg cttccagccg ccaccaccgc caaaataggc aatacgtaaa
    25561 ccagccataa caatcgacat aatcactgcg cccagcggtg tgtctccacg ccaccagctc
    25621 tggaacaact ccagccaagt atttgggtta tgaggcattt gtagttatct ctcacctcgc
    25681 caatacagga ggtgccaatt gagggaatac cacgtaccgc acatcagaag cggaaacgtc
    25741 aaagaagccg agtcaatgga taaatgtggg atagaccagg accaacgaat ccccagccca
    25801 agaaacgaca aaacccgctc gacggcgggt ttaagctgtg tggcgaagta accactctta
    25861 acacagtaat ctagaaaatg cggaccgcgt tagtgatttt ttcaactttt tgatttatct
    25921 tattgaacac aagaaaccac tgcctataaa tcatatcaac ccaaaccata aaaaccaaaa
    25981 aatcaaatga ttacattaac tagataagga acaaagttaa tgaaagaaag ccgcagcaag
    26041 gtcattcaat ataaacgtgc agtcatccct aattgcacag caacgcttca acagatcata
    26101 gaatctataa tttctgaaaa tggcgcagcc tataaagtta gcgcaagaag agaacagatt
    26161 aacccctccg atagcaatag tggatttaga gtggttaacc gcagcagcac tttcaagaca
    26221 gtgttattcg ggcaacttat tttgtttgaa caaggtaaaa gccaaacact tatgacaatc
    26281 gctgatgatg tcaattatta tgacatcaat gctataacat cgaagcaaat taaattagcc
    26341 gaagacgaaa aaataagtga agctgaaaaa caaaaaataa cacgagaatt tattgattca
    26401 atactttact ttgggattcg tgataatcat gtgatgatcg tccaatcacc ggctctaagg
    26461 actaaagaca tagaaagcca tcttaattgg ttaatccatt catttagcac tatttttaat
    26521 ggtgacgact tccttgtact tcaagataaa ccaactgaag acaccataaa aaaaatgcat
    26581 gaaagtccag tcaagaaaat aaatttcggc agtatcccaa tcaaaaattc tgattctgaa
    26641 gatgtagtta aaataaataa tataagtaac acaaacgacc tccccacaac aataaaagaa
    26701 caggtcgaaa aagttaagaa aataaaattc atgccaactg gtaaaggtgg cgatattata
    26761 aaagctgctt ttggcgaagc atggtttaat aatttacaac tggaagattc tcttgatgaa
    26821 tccaatttac aagttaatct agaaataacc tatttccgca agacaaacaa agatggtcaa
    26881 cgtgtactcg atactttagc aacttcactc agaaatatag acgatgaaga tattagtata
    26941 aaccttcaag gtggtggcat aattaaaggc agtgatttaa aactatccgg taaagtaaac
    27001 gtccaatata acaatggact tattgatgaa aatgatttat atttacaaat ggctgacttc
    27061 aaaggttcaa actggagaag taaaagttaa aaactagaac caacaggagg cggaaatgag
    27121 cttctttaaa tttattataa gattcttatc cgccttcgct tttgggtttg caatatttta
    27181 ctttgcatct agaaaaattg acttaagtgg catatctact ccatggtcat taataacctt
    27241 attagcattc cctttcagtt attgtatcgc agcctttttt aaagtttctg aagccgacga
    27301 aaacacatca ctatcagata atgaactaag aagattacga ccgataattg atgcaaaaaa
    27361 aagacatctt ggatttctta tcttttttta tcttattgct gctttttcag gagcaattgg
    27421 ggtaatgact ccaacttatt gatagtgttt tatgttcaga taatgcccga tgactttgtc
    27481 atgc
//
LOCUS       DAJVPP010000054        27336 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVPP010000054 DAJVPP010000000
VERSION     DAJVPP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.054"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OQM34_002266"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OQM34_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1452695.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OQM34_002267"
     CDS             complement(1614..1937)
                     /locus_tag="OQM34_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1452696.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQM34_002268"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQM34_002268"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1452697.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQM34_002269"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQM34_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1452698.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQM34_002270"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQM34_002270"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1452699.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQM34_002271"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQM34_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1452700.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OQM34_002272"
     CDS             7537..8574
                     /locus_tag="OQM34_002272"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1452701.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQM34_002273"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQM34_002273"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1452702.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OQM34_002274"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OQM34_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1452703.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQM34_002275"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQM34_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1452704.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OQM34_002276"
     CDS             10745..11626
                     /locus_tag="OQM34_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1452705.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQM34_002277"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQM34_002277"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1452706.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OQM34_002278"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OQM34_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1452707.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQM34_002279"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQM34_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OQM34_002280"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OQM34_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1452708.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OQM34_002281"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OQM34_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1452709.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OQM34_002282"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OQM34_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1452710.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OQM34_002283"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OQM34_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1452711.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OQM34_002284"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OQM34_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1452712.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OQM34_002285"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OQM34_002285"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1452713.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OQM34_002286"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OQM34_002286"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1452714.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OQM34_002287"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OQM34_002287"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1452715.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OQM34_002288"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OQM34_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1452716.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OQM34_002289"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OQM34_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1452717.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQM34_002290"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQM34_002290"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1452718.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OQM34_002291"
     CDS             24893..25273
                     /locus_tag="OQM34_002291"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1452719.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OQM34_002292"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OQM34_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1452720.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQM34_002293"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQM34_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1452721.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27336
                     /locus_tag="OQM34_002294"
                     /pseudo
     CDS             26442..27336
                     /locus_tag="OQM34_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6735 a         6808 c         7170 g         6623 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcttgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgga ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctggggcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggtgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc ctggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgca cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttat atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggc
//
LOCUS       DAJVPP010000055        27261 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVPP010000055 DAJVPP010000000
VERSION     DAJVPP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.055"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..131)
                     /gene="yjdI"
                     /locus_tag="OQM34_002295"
     CDS             complement(<1..131)
                     /gene="yjdI"
                     /locus_tag="OQM34_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCS1452722.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNL"
     gene            312..1943
                     /gene="dcuS"
                     /locus_tag="OQM34_002296"
     CDS             312..1943
                     /gene="dcuS"
                     /locus_tag="OQM34_002296"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1452723.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1940..2659
                     /gene="dcuR"
                     /locus_tag="OQM34_002297"
     CDS             1940..2659
                     /gene="dcuR"
                     /locus_tag="OQM34_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1452724.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3230..4570
                     /gene="dcuB"
                     /locus_tag="OQM34_002298"
     CDS             3230..4570
                     /gene="dcuB"
                     /locus_tag="OQM34_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1452725.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4648..6294
                     /gene="fumB"
                     /locus_tag="OQM34_002299"
     CDS             4648..6294
                     /gene="fumB"
                     /locus_tag="OQM34_002299"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1452726.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6417..7046
                     /locus_tag="OQM34_002300"
     CDS             6417..7046
                     /locus_tag="OQM34_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1452727.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7185..8606)
                     /gene="melB"
                     /locus_tag="OQM34_002301"
     CDS             complement(7185..8606)
                     /gene="melB"
                     /locus_tag="OQM34_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS1452728.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8709..10064)
                     /gene="melA"
                     /locus_tag="OQM34_002302"
     CDS             complement(8709..10064)
                     /gene="melA"
                     /locus_tag="OQM34_002302"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS1452729.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10347..11255
                     /gene="melR"
                     /locus_tag="OQM34_002303"
     CDS             10347..11255
                     /gene="melR"
                     /locus_tag="OQM34_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS1452730.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11454..13721
                     /gene="adiA"
                     /locus_tag="OQM34_002304"
     CDS             11454..13721
                     /gene="adiA"
                     /locus_tag="OQM34_002304"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1452731.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14045..14806
                     /gene="adiY"
                     /locus_tag="OQM34_002305"
     CDS             14045..14806
                     /gene="adiY"
                     /locus_tag="OQM34_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1452732.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14943..16304
                     /gene="adiC"
                     /locus_tag="OQM34_002306"
     CDS             14943..16304
                     /gene="adiC"
                     /locus_tag="OQM34_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1452733.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKDKFPLI
                     QQT"
     gene            16384..18027
                     /gene="eptA"
                     /locus_tag="OQM34_002307"
     CDS             16384..18027
                     /gene="eptA"
                     /locus_tag="OQM34_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1452734.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18024..18692
                     /gene="basR"
                     /locus_tag="OQM34_002308"
     CDS             18024..18692
                     /gene="basR"
                     /locus_tag="OQM34_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1452735.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18693..19793
                     /gene="pmrB"
                     /locus_tag="OQM34_002309"
     CDS             18693..19793
                     /gene="pmrB"
                     /locus_tag="OQM34_002309"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1452736.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19769..19858)
                     /gene="pmrR"
                     /locus_tag="OQM34_002310"
     CDS             complement(19769..19858)
                     /gene="pmrR"
                     /locus_tag="OQM34_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1452737.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19970..21472)
                     /gene="proP"
                     /locus_tag="OQM34_002311"
     CDS             complement(19970..21472)
                     /gene="proP"
                     /locus_tag="OQM34_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1452738.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21736..22614)
                     /locus_tag="OQM34_002312"
     CDS             complement(21736..22614)
                     /locus_tag="OQM34_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCS1452739.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22611..24839)
                     /gene="crfC"
                     /locus_tag="OQM34_002313"
     CDS             complement(22611..24839)
                     /gene="crfC"
                     /locus_tag="OQM34_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1452740.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIVERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25241..25576
                     /locus_tag="OQM34_002314"
     CDS             25241..25576
                     /locus_tag="OQM34_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1452741.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25734..26177
                     /gene="yjdN"
                     /locus_tag="OQM34_002315"
     CDS             25734..26177
                     /gene="yjdN"
                     /locus_tag="OQM34_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCS1452742.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26310..27098
                     /gene="phnC"
                     /locus_tag="OQM34_002316"
     CDS             26310..27098
                     /gene="phnC"
                     /locus_tag="OQM34_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1452743.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALC
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27123..>27261
                     /locus_tag="OQM34_002317"
     CDS             27123..>27261
                     /locus_tag="OQM34_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="with PhnCE is involved in the transport of
                     phosphonates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1452744.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NL"
BASE COUNT         7009 a         7100 c         6614 g         6538 t
ORIGIN      
        1 ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg acatatcgca
       61 gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc gtccagtagc
      121 gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc atagccccgg
      181 aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat atagccaatc
      241 atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa atttgtcaca
      301 caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt ccgatgaaat
      361 tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta ttggtggtgc
      421 atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc aacaaggcac
      481 tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg cagaaaaaac
      541 cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac gatctgctgt
      601 ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc cagcgtattg
      661 gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa aatgtcgcta
      721 tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac gatgaaaatc
      781 ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc caacagatca
      841 atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc ggactgattg
      901 gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa ccctacgaaa
      961 tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa ggcgtcgttg
     1021 ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa ttgctgaatt
     1081 accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg tcacaggtgg
     1141 tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag attacgatta
     1201 aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt atcatcggtg
     1261 ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga ctcgacggtc
     1321 tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat aaattgcatg
     1381 tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac attctcaaaa
     1441 cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa tctccggtta
     1501 tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat acgctgattt
     1561 taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg acgctgatta
     1621 ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa cccggtggcg
     1681 aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt aacgatgatg
     1741 gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc tcgacaaaag
     1801 gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat ctcggcggca
     1861 gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag ataccctggg
     1921 acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg caatggtcgc
     1981 ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg gaacagcctc
     2041 gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg acctgatatt
     2101 gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc tgcataacgc
     2161 gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa ccattaaaga
     2221 ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt cccgctttga
     2281 agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc agtattatga
     2341 ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg atcctcgtcg
     2401 cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga ttgacgcgca
     2461 tcaggactat gagttttcaa ccgacgagct ggctaacgag gttaacatct cgcgtgtttc
     2521 ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca gtatccatta
     2581 tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact actcactgct
     2641 gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta ttaccgtaat
     2701 gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc agatcaaata
     2761 taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg tgtttttaat
     2821 ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt gtatttttac
     2881 ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct gagaacaaaa
     2941 aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc tgcgcagtac
     3001 aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta tcacagtgca
     3061 taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct aaagccggta
     3121 acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca gaacgtgact
     3181 gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca tgttatttac
     3241 tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg gtggtatcgc
     3301 gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc accttcagcc
     3361 aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg catcggcgac
     3421 cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc tgctgcgccg
     3481 caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga ccattctttg
     3541 cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg ccattaagaa
     3601 caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga tggggattat
     3661 cgccagtccg gtgtcggttg cggttgtgtc tctggttgcg atgctgggta atgtcacctt
     3721 tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat cgacgttaat
     3781 cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg ataaagacga
     3841 agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg gtgataccgc
     3901 gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga ttttcctcgg
     3961 ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat ccttcggcgg
     4021 caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg gggcgctgat
     4081 tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct tccgttccgg
     4141 tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca tgttcggtgc
     4201 gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc cgtgggcgta
     4261 tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg cgctggcggc
     4321 gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg cttcagcacc
     4381 ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag cgattcagtt
     4441 tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt ttattctgcc
     4501 ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg cgatgtacgg
     4561 gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat tttcgaataa
     4621 caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta ccaggcacct
     4681 ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta cgttagcgtt
     4741 gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac cctgctggcg
     4801 cagcaagcct ttcacgacgc ttctttcatg ctacgtccgg cacaccagaa acaggttgcg
     4861 gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact gcaattctta
     4921 agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac cggcaccgcg
     4981 atcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga agaagcgctg
     5041 tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca aaacgcggcg
     5101 ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat cgacctgtac
     5161 gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg ctctgccaac
     5221 aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact gaaaaacttc
     5281 ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca tatcgcgttt
     5341 gtgattggcg gtacgtctgc ggaaaccaac ctgaaaaccg tcaaattagc aagcgctcac
     5401 tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg cgatgtccag
     5461 ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca gtttggcggt
     5521 aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc atcctgcccg
     5581 gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat caaccgcgaa
     5641 ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca agaactgcgt
     5701 caggctggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa agagatcctc
     5761 gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac cattattgtg
     5821 ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa agaacttccg
     5881 cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc tgccggttat
     5941 ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt ggatctgctg
     6001 caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca gcaggttacc
     6061 gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc ggcggcggta
     6121 ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct gggtatggaa
     6181 gctatctgga aaatcgaagt agaagatttc ccggcgttta tcctggtcga tgacaaaggt
     6241 aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa gtaacctctt
     6301 cggcccagcg cctggcagca tgctgccagg tgatccccct gggccacctc ttttgcgatt
     6361 gtaatttcat gcttgctggt gaatagtcag tattttccct gacttgcgaa ctcaccatga
     6421 cccgcacact caagccgtta attcttaaca ccggcgcact ggcgctaacg ttaatcctga
     6481 tttataccgg catttcggcc catgacaaac tcacctggct gctggaagtg acaccggtga
     6541 ttatcgtcgt gccgctacta cttgccaccg ccagacgtta cccgttaacg ctgctgctct
     6601 atacgctcat cttctttcac gccatcatcc tgatggtagg cgggcagtac acctacgcga
     6661 aagtccccgt tggttttgag gtgcaggaat ggctggggtt gagtcgtaat ccgtatgaca
     6721 agctggggca ctttttccag gggctggtgc ctgcactggt ggcacgagaa attctcgttc
     6781 gcgggatgta cgtgcgcgga cgtaaaatgg tggcgtttct ggtctgttgc gtggcgctgg
     6841 cgataagcgc gatgtatgaa ttaatcgagt ggtgggcggc actggcgatg ggccagggag
     6901 cggatgattt tctcggtacc cagggcgacc agtgggacac gcaatccgat atgttctgcg
     6961 cgctgcttgg cgcattaacg acagtgatat tgctcgctcg ctttcattgc cgccagctac
     7021 ggcgctatgg cttgataact ggataacaac gtgcactttg ttgatgccgg atgcggcgtg
     7081 aattccttat ccggtctaca aaatttgcaa atccaaagca ttacattaag acgtggcctg
     7141 ataaacatat cgcatcaggc cattttcgtt agctattgtc cacgtcaggc tttcacatca
     7201 ctcactgcac cgaccggaat atcagcatga acaggctcgg gcggtacttt gcgatattta
     7261 tccagcagat gaatctggat cctgcgcagc gtgtcgccat tgaggcgata gaaacggaag
     7321 tagagaatca gcgttaccat gaaaaacaga gtcggtagag caatcataat aaactgcata
     7381 cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat ccctaacaca
     7441 accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt ctgcacggag
     7501 taagcgatgc tttcacagcg tacgtgcagt ttatattcac cgtaatcaac ggtatctgcc
     7561 accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa aatacccgca
     7621 atcacaatga ggacgacgtt gtgatagctc attaatgcca ttaacaggag aacaccacag
     7681 cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa tgatttaact
     7741 aagcgtggga agaatactaa cgtcaccagg ttagcagctc ccgcatacga cagataatag
     7801 gggaacaaat ccgcatcacc gataacatat gagaaataat agatagcaaa tccggtaata
     7861 atgttgctgg ctacattata agcaagagcc atacccaaga ggcatgaaag ctgatcgttt
     7921 ttataaataa gcgcaacgat ggctttaagt gtcagatggc ttccttcagc agacgattga
     7981 ttgtctgacg aaaagacttc atgcacattg cgcagagtga tgatggttga aacaataaaa
     8041 aaggcgatca ataccagagt gaacatctga aagccaaatc cccgatcgcc accgccgaca
     8101 taattaacaa atggtagcgt cacacctgcc gtaacaaagc ctgccagact ggcaaaaaaa
     8161 cgcggataag gaaccagttg ttcgcgttca cgtttatcga gcgtgatggt tggaaccagc
     8221 gaccagaagg gaatatccat aatggtgtaa gtcatgcccc agaggatgta ggtcacgcaa
     8281 acaaagacaa tctgagtagt accttcaaac agatgcgcac taaagaggag aaataagatt
     8341 acagagtttg ccaacgtacc gatcaggatc cagggtttga acttacccca tcgcgatcgc
     8401 gtagcattta caatccatcc cataatcgga tcgttaatag catcccagat cctcgccacc
     8461 agaaacaaag taccgaccaa acccacagac agcccgacga catcggtgta gtaatacatg
     8521 aggtacatat acacaatgcc gatcgcaaaa tccttcccga acgctccaaa tccataactg
     8581 agttttgtag tcattgaaat gctcataggg tatcgggtcg ctgttaccaa cgcccgcctt
     8641 ctgttattgg tatcgtcaga ggtgtgcgcc ggaataaatc ccccggcgca gtagcgttta
     8701 gtcgcgtttt aacggtgcaa ccagcttggc agccagtcgc cgtgggcggc aatcaggtcg
     8761 tcaacaagag catatatttc gtcaatgccc agcacggcgg cagtatgcgg gtccatcatc
     8821 gcggcgtggt aaacacggtc gcgattttcc gtaagaatag cttcggtcag cagcgtctgt
     8881 acgttgatgt tggtttgcat cagggcggcc agatgcgaag gtagcgtacc gactttggtc
     8941 ggctgaatgc cattagcatc aaccagacag gctacttcca cgcaacatcc ttgtggcagg
     9001 ttatcaatca aaccatcgtt acggacgttg ccgtaaatca cactcggctc gccagtccag
     9061 atagcgttca tgattgtgct ggcatattcc cgtgacggtt taatatcaat ccgggaggcg
     9121 tttttatact cctccagctc tttatgccag ttcgccagct gctcgacgca gcgtttcggg
     9181 tactcatcca gcggtacttt ataacgctca atcaaatcct cacgacctag cttaataaac
     9241 cacggcgtat attcggcaaa atgttctgac gattccgtga cgaagtagcc cagctttttg
     9301 aacatttcgt agcgcacaat attctggcag cgagtattac catgaatatt tggcttcggt
     9361 gcctgccctg cttcataagc cgccagcagt tccgggtaga gattcacgta actgccgtcg
     9421 gcggttttgc gctccagctc caggtaaaac gccatatggt tgatacctgc gcaacggtaa
     9481 cgcagcgtag ccgggtcgat attgaggtca cgcgccagct cttccgccgt tccctgcacc
     9541 gaatggcaca gcccgacctg tttgatatac ggatagcggg catacatcgc ccaggtattc
     9601 atcgccattg ggttaacata gttgagcatg gtggcatcgg ggcagacttc cgtcatgttc
     9661 tcgcaaattt gccacagatg cggaatggta cgtagcgcgc gcataatacc gcccggcccc
     9721 aacgtatcgg caatggtttg ttccagacca tgccgcttac agacctcgaa atcagtcacc
     9781 gtgcaaggtt cataaccgcc aatctgaaat gccaccacga caaaatcggc atcctctaag
     9841 gcttctttct gttgggtgtg gcaggtgatt ttgccgctgg cccctgctga atccatcagc
     9901 ttacgcacca caatatgcga ctcttccagg cgggtggggt caatgtccat cagggcaata
     9961 tgcgccgttt tcagcgcctc gcgatggaac acatcaccaa gaatattttt aacgaaaatc
    10021 gtcgaaccag cgccgataaa tgtaattttg ggtgcagaca tcatgcagat ctcctggctt
    10081 gcttgaataa cttcatcatg gcaggcgaat ctgcagaaaa cctccggctt ccaggttgag
    10141 cattcccata aactcagatc ctgcgtgaag cagcagtaaa tctgagttta tgggaataaa
    10201 ttacctgaaa agtagagttt tcggcctcat gatgctcgga cagtcccctc gcccctctgg
    10261 gaagagggtg aaaaccgtgc tcccactcgc agtcatcctc cctcactcct gccataattc
    10321 tgatattcta ggaaagagag ccatccatga atacagatac gtttatgtgc agcagcgacg
    10381 aaaaacagac ccgcagcccg ctgtcgctgt attcggaata ccagcggatg gagattgagt
    10441 ttcgcgcacc gcatatcatg cccaccagcc actggcatgg tcaggtcgaa gtgaatgtgc
    10501 ctttcgatgg cgatgtggaa tacctgatca acaatgaaaa agtgaatatc aatcagggtc
    10561 atatcacgct gttctgggcc tgtacaccgc accaactaac agataccgga acctgtcaga
    10621 gcatggcgat ttttaatctg ccgatgcatc tgtttctctc ctggccgctg gataaagacc
    10681 tgattaacca cgtcactcac ggcatggtga tcaaatcact tgccacacag caacttagcc
    10741 cgtttgaagt acgccgctgg cagcaggaat taaacagtcc gaatgagcaa attcgccagc
    10801 tcgccattga tgaaattggc ctgatgctca agcgatttag cctctctagc tgggaaccga
    10861 ttctggtcaa taaaacctcg cgcacacaca aaaacagcgt ctcgcgccat gcgcaatttt
    10921 atgttagcca gatgctgggc tttattgccg aaaactatga tcaggcgctg accatcaacg
    10981 atgtggctga gcacgtcaaa cttaacgcca actatgcaat ggggatattc cagcgggtca
    11041 tgcaattgac gatgaaacag tacattaccg cgatgcgcat caaccacgtt cgcgcgttac
    11101 tgagcgatac cgataaaagt attctcgata ttgccctgac ggcaggcttt cgttcaagta
    11161 gtcgttttta cagcacgttc ggcaaatatg tcggcatgtc gccgcaacaa taccgcaaac
    11221 ttagccaaca gcgccgccag acgtttcccg gctaaagacg atatcagtat cagccaaaaa
    11281 aatagtttag ccgtcgataa taagcagtga aaataattta aaataatcac ataacgtatt
    11341 gtttataaaa ctaaatattt caaaaatgtt tgtttttcac gcgctttaca gcccgaaaag
    11401 gccggaagat acttgcccgc aacgaagatt ccttcataac cgggtaagca atgatgaaag
    11461 tattaattgt tgaaagcgag tttctccatc aagacacctg ggtcggtaac gccgttgagc
    11521 gtctggcaga tgctttaagc cagcaaaatg ttaccgtgat taaatccacc tcctttgatg
    11581 atggttttgc cattctctct tcaaacgaag ccattgactg cctgatgttc agctatcaaa
    11641 tggagcatcc ggacgaacat caaaacgtca gacaattgat cggtaagctt catgagcgcc
    11701 aacaaaacgt gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc gcaatggacc
    11761 gggatctgct ggagcttgtc gatgaattcg cctggattct ggaagatacc gccgacttta
    11821 tcgccggacg cgccgttgcc gcgatgaccc gctaccgcca gcagctgttg ccgccactgt
    11881 tcagcgcgct gatgaaatat agtgacatcc atgaatattc ctgggcagcg ccaggccacc
    11941 agggcggcgt tggttttacc aaaacacccg ccggacgttt ctaccatgac tactatggtg
    12001 aaaatctgtt ccgcaccgac atgggcatcg aacgaacttc cctcggttct ttgcttgacc
    12061 atactggcgc atttggcgaa agcgaaaaat atgccgcacg cgtatttggt gccgatcgct
    12121 cctggtcggt agtcgtcggt acttccggct ctaaccgcac catcatgcag gcttgcatga
    12181 ccgataacga tgtcgtggtc gttgaccgta actgccataa atccatcgaa caaggtttga
    12241 tgctgacagg cgcgaaaccg gtctatatgg tgccaagccg caaccgctac ggcattatcg
    12301 ggccaatcta tccgcaggaa atgcaacctg aaaccttgca aaagaaaatc agtgaaagcc
    12361 cgctgaccaa agacaaagcc gggcaaaaac cgtcttactg cgtggtaacc aactgcacct
    12421 atgacggcgt gtgttataac gctaaagaag cgcaggatct gctggaaaaa acctccgatc
    12481 gtctgcactt tgacgaagcc tggtacggct atgcacgttt caacccgatc tatgccgatc
    12541 actatgccat gcgcggcgaa cctggcgatc acaacggtcc taccgttttc gccacccact
    12601 ccatccacaa actgctgaat gcgctgtcac aggcttctta tattcatgta cgtgaaggtc
    12661 gtggggcgat taacttctcc cgcttcaacc aggcctacat gatgcatgcc accacctccc
    12721 cgctgtatgc catctgcgca tccaacgacg tggcggtgtc gatgatggac ggcaacagcg
    12781 gcctgtcact gacacaggaa gtgattgacg aagcggttga tttccgtcag gcgatggcgc
    12841 ggctatataa agagttcacc gctgacggta gctggttctt caaaccgtgg aacaaagaag
    12901 tcgtcaccga cccacaaacc ggcaaaacct atgactttgc tgacgcacca accaaactgc
    12961 tgaccaccgt tcaggactgc tgggtaatgc atccgggcga aagctggcac ggcttcaaag
    13021 atattccgga taactggagt atgctcgacc caattaaagt cagcatcctt gctccgggaa
    13081 tgggtgaaga tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc actgcctggc
    13141 ttggtcgcca cggcattgtg cctacccgca ccactgactt ccaaattatg ttcctgttct
    13201 ctatgggcgt aacccgtggg aaatggggga ctctggttaa caccctttgc tccttcaaac
    13261 accactatga cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt gaacaatatc
    13321 ctgacactta cgcgaacatg gggattcacg atctgggtga caccatgttt gcctggctga
    13381 aagaaaacaa ccctggcgca cggttgaacg aagcctattc cggcctgccg gtggcggaaa
    13441 tcaccccgcg tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg gtatccattg
    13501 aaaatctgcc aggacgcatc gcggcaaact cagttatccc gtatccgcca ggaatcccga
    13561 tgctgctgtc tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt tatttacgct
    13621 cgctgcaatc ctgggaccac catttccctg gatttgaaca cgaaactgaa gggactgaaa
    13681 ttattgacgg tatttaccac gttatgtgcg tgaaagcgta accactattc cgctgaaggc
    13741 gtaattgttt aaataacatt acgccgcctg gccttaggcc ttttgagtat ggcaacgttt
    13801 tcataaaaat tgctgcaaac aaaaatgtca tactttttgc gcggccccac cccgcgcttt
    13861 ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta taaaaatttg
    13921 taatattagt aaaactcgtt atttttatgc atgtttatat tcatcatgca attatataac
    13981 catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac tcaggagtac
    14041 atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa aagatgtctg
    14101 gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg cgttattaaa
    14161 ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg ctcatattag
    14221 tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc aaataccagt
    14281 atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag acgtctttcg
    14341 cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg aacgaacacg
    14401 cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc tttcattaat
    14461 gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg aaagcgatat
    14521 tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc caagtctgtt
    14581 aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca cctgccgcat
    14641 gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg tatcacagtc
    14701 ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct acggtatgac
    14761 gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt taaccttaac
    14821 gaagagctat attaataacg gcatcagcga taacccggtc gataataatt caactatcga
    14881 atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc aggagtttaa
    14941 ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc ctgatggtgt
    15001 cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc tccactggcg
    15061 ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc tcgatggtat
    15121 acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac gcccgccgct
    15181 gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc tgctggatcg
    15241 gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc ccgattctga
    15301 aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc gtcctgctga
    15361 acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg ctggcgctga
    15421 tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa acctatatgg
    15481 cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc cttaacgtta
    15541 cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg gtgaaaaacc
    15601 cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc gtttgctatg
    15661 tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc gtttctgcct
    15721 cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc attgtttcct
    15781 tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg gcgggtcaaa
    15841 cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt gtaaataaag
    15901 cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc ttccagctca
    15961 gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc tcggtcatct
    16021 ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc cacggtcact
    16081 ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc tactgcattt
    16141 gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc ctgatggtca
    16201 tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat cccttagatg
    16261 caccaataag caaagataaa ttccccttaa tccagcaaac ataaaagcca accttaagaa
    16321 cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga tggaaacacc
    16381 gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg gctattgttg
    16441 gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt gttgcaggcg
    16501 ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt cgtcgctttc
    16561 agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg accactggcc
    16621 tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta cggcatcgtc
    16681 atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag ttatgcgctg
    16741 atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctgc gctgattgcc
    16801 tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt ccgtggagcc
    16861 aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta taaagactac
    16921 gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc taacagcatt
    16981 gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct ggtgcgaatt
    17041 ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa tttgaccatc
    17101 ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta cccgcgtgaa
    17161 actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc atcttgcggc
    17221 acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga gcactacaaa
    17281 gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc gggcatcaac
    17341 gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt gcctcaccag
    17401 aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta tgacgaagtg
    17461 ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt gattgtctta
    17521 cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca gttcaggaaa
    17581 tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca actggtgaac
    17641 acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat taatctgctt
    17701 aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca cggtgaatcg
    17761 ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc ggatagccaa
    17821 aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta tcaggttgac
    17881 cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa tttattctcc
    17941 acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga tgatattctg
    18001 caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat acgctgttat
    18061 tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc gtgacaaccg
    18121 cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta ctggatttag
    18181 ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa aaatacaccc
    18241 tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc gggctggatg
    18301 tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac gcccgtatcc
    18361 gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt ggcaatctga
    18421 cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt ctgacgccca
    18481 aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta catcgggaaa
    18541 ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc ctggaagtgc
    18601 atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg cgcggctttg
    18661 gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt ctgcgccgac
    18721 caatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg gtgtttgagc
    18781 tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg tttgagcagg
    18841 cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc gaggcggtcg
    18901 ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc tgctatcagg
    18961 cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa gcgcgcaccg
    19021 ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa gcggtggttt
    19081 cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa aggttgttta
    19141 ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg catctggaac
    19201 tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt gatcagatga
    19261 tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt tcttccggta
    19321 attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac gaactcagta
    19381 ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc gacatcaccg
    19441 ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa aacgcccatc
    19501 gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc ggagcggtca
    19561 tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag ttgagtaaag
    19621 cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt attgtcagcc
    19681 gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag acttccggca
    19741 cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata taaagaaagc
    19801 tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg tttttcattg
    19861 cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg aaccttaaga
    19921 gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct tattcatcaa
    19981 ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca atctcgtggt
    20041 caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt tccttcgctt
    20101 cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc gtctctttca
    20161 tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag gcaggcatca
    20221 tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga ccggcaacca
    20281 gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc gggaacatcg
    20341 ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc agcatcagta
    20401 acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc gggatcgcca
    20461 acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa cggtcactca
    20521 gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata ataatcagca
    20581 ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata taggtcagca
    20641 acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac aggctgcgcc
    20701 agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa ccttcgcggt
    20761 cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc agcgcatggc
    20821 gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata cgccagcccc
    20881 agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg cccgcaccca
    20941 gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca cgtttacggt
    21001 caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg accgagaaac
    21061 cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc gtgtcgtagg
    21121 acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg atagcgagga
    21181 tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca agcggtcgaa
    21241 tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg gggtcagccc
    21301 ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca aaatcaaacc
    21361 attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta ccgtcatcaa
    21421 taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc atagctttcc
    21481 tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc cgcaaaaggg
    21541 gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct tacaccctcc
    21601 ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa tgaccttttt
    21661 accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt aggactcatt
    21721 gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca ttgcccccac
    21781 caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc agcgttgggt
    21841 cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc aacttaaaaa
    21901 atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat ggtaatagtg
    21961 acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt tctcgcgcaa
    22021 atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat cgccaaatgc
    22081 accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc aggcgttata
    22141 cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct gcaccagtag
    22201 gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc actcatacaa
    22261 ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat ccattttttg
    22321 cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat ccagcatcga
    22381 cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca tgccgccgtt
    22441 cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt cacgaaactg
    22501 ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca ctaaaaagcg
    22561 cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg tcaatatcgt
    22621 tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt gtaaatccag
    22681 ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac cgattcattt
    22741 tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa acatgaagcg
    22801 aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg ctctgccaga
    22861 actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg ttgcaacaca
    22921 ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc gggctgattc
    22981 aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc gtccgttgct
    23041 tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg aactacgccg
    23101 tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc cattttgact
    23161 tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga ttccatctcg
    23221 gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa cagtgtctca
    23281 cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc ggctaactgc
    23341 gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag ggccagctcg
    23401 atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag ctgcaaactt
    23461 tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa cccgtgcgca
    23521 cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg cgcagcagat
    23581 cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg aattggctgg
    23641 gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc gaggtccgcc
    23701 agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc cacccagcgt
    23761 tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc ccgattcgcc
    23821 aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca gcctttcatc
    23881 agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg atcctgttga
    23941 tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa aatcgcctca
    24001 cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc cagtaccgcc
    24061 gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc ggcttcattt
    24121 ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc cgccagatgg
    24181 acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga aaaaggaaaa
    24241 tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag acaatgaaaa
    24301 ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat ccgctgcata
    24361 agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat atcgcaatca
    24421 cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg tgaaaaatgc
    24481 agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc agtcattggg
    24541 cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt tgatttccct
    24601 gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt acacaactca
    24661 ttattcagca tcgcgtgacg ttcgacgata ttaggctgac tccagggtaa agccagttgt
    24721 ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa ctgttggcga
    24781 gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg tgtgtacata
    24841 gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt ttttgagttc
    24901 tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg ttgtttttta
    24961 tcagaataaa taacgtcttc tgatgcgttt aaaacgtcag aaagataaaa atatcatgtg
    25021 aataaaaaaa agaacaagta gagcattaac attatcttaa ataataaata gaggcaaaaa
    25081 gattattttc tttttgcgtt tcctttcaaa tgaaaacgat cgtcgtctaa aatcagcagt
    25141 acccccgaca aactcaggga ttttgtgtat aattgcggcc tttttcggca atctgccgtt
    25201 ttttggcgct tttgccctgc tgacttttga ggaaatccac atgtcattac cacactgccc
    25261 aaaatgcaac tccgaataca cttacgaaga taacggcatg tacatctgcc cggaatgtgc
    25321 ctacgaatgg aacgacgcag aacctgcaca ggaaagcgac gagctgatcg ttaaagatgc
    25381 taacggcaat ctgctggctg acggcgacag cgttaccatc attaaagatc tgaaggtgaa
    25441 aggtagctct tcgatgctga aaattggcac caaagtgaaa aacatccgcc tggttgaagg
    25501 cgaccataac atcgattgca aaatcgacgg ttttggtccg atgaaactga aatctgagtt
    25561 tgtgaaaaag aactgattgt attgtgatcg gtaagccgga taaggcgctc gcgccgcatc
    25621 cggcaacgat gccgactgcc tgatgcgacg cttgcgcgtc ttatcaggca gcctctcatc
    25681 tgtataaatt tcgaactaca cttaactggc ttctcttaac tgaggtcacc atcatgccgt
    25741 taagtcccta cctctctttt gccggtaact gttccgacgc gattgcctat tatcaacgta
    25801 cgttgggcgc ggaactgctc tataaaatca gcttcggcga aatgccgaaa tcagcgcagg
    25861 acagcgccga gaactgccct tccggaatgc aatttcccga taccgccatc gctcatgcca
    25921 acgtgcgcat tgccggaagc gacatcatga tgagcgatgc tataccgtca ggaaaagcca
    25981 gctactccgg ctttacgctg gtgctcgatt cgcaacaggt cgaagaagga aaacgctggt
    26041 ttgataacct tgccgctaac ggaaaaatcg aaatggcctg gcaggaaact ttctgggcgc
    26101 atggctttgg caaagtcacc gataaatttg gcgtaccgtg gatgattaat gtcgtcaaac
    26161 aacaaccaac gcaataaccc gccgggaggc ccgccctccc gcactgtcat cgaattcccg
    26221 ttaactcttc atctgttagt cacttttaat taaccaaatc gtcacaataa tccgccacga
    26281 tggagccact tttttaggga ggctgcatca tgcaaacgat tatccgtgtc gagaagctcg
    26341 ccaaaacctt caatcagcat caagcgctgc atgcggttga tctgaacatt catcacagtg
    26401 aaatggtggc tctgcttggg ccgtcgggtt ccggcaaatc caccctttta cgtcatttaa
    26461 gcggtttgat taccggcgat aaatccgccg gtagccatat cgagctactg ggccgcactg
    26521 tccagcgcga aggccgcctg gcccgcgata tccgcaaaag ccgcgccaac accggctaca
    26581 tctttcaaca attcaacctg gtgaaccgcc tgagcgtact ggagaacgtg ctgattggcg
    26641 cgctcggcag cacgccgttc tggcgcacct gttttagctg gttcaccggc gagcagaaac
    26701 agcgcgcgtt acaggcgctg acccgcgttg gcatggtgca ttttgcccat cagcgcgttt
    26761 ccaccctctc cggcggccag cagcaacgtg tggcgattgc ccgtgcgctg atgcagcagg
    26821 cgaaagtgat tctggccgat gaacccatcg cctcgctgga cccggaatca gcgcgcatcg
    26881 tgatggacac cctgcgcgac atcaaccaga acgacggcat caccgtggtc gtcacgctgc
    26941 atcaggtgga ttacgccctg tgctactgcg aacgcatcgt cgccctgcgt caggggcacg
    27001 tcttctacga cggcagcagc caactgtttg ataacgaacg ttttgaccat ctctaccgca
    27061 gcattaaccg catcgaagag aacgcgaaag ctgcctgata tccccatcat tgaggaaaac
    27121 gaatgaacgc taagataatt gcctcgctgg ccttcaccag catgttcagc ctcagcaccc
    27181 tgttaacccc ggcacacgcc gaagagcagg aaaaggcgct gaatttcggc attatttcaa
    27241 cggaatcaca gcaaaacctg a
//
LOCUS       DAJVPP010000056        27185 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVPP010000056 DAJVPP010000000
VERSION     DAJVPP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.056"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            38..2251
                     /gene="tssI"
                     /locus_tag="OQM34_002318"
     CDS             38..2251
                     /gene="tssI"
                     /locus_tag="OQM34_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1452745.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHEGRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            2274..2719
                     /locus_tag="OQM34_002319"
                     /pseudo
     CDS             2274..2719
                     /locus_tag="OQM34_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            2741..3148
                     /locus_tag="OQM34_002320"
     CDS             2741..3148
                     /locus_tag="OQM34_002320"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCS1452746.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            3145..5622
                     /locus_tag="OQM34_002321"
     CDS             3145..5622
                     /locus_tag="OQM34_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1452747.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWSQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDRSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELVKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDEQGLAVSRTDARGGEVALAHDARGQLRRYTDCSGCATGYE
                     YDEDGNLTAVTDAEGKTVRISYNRQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGS
                     VQSWQYTPRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQ
                     RPDGLIRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNR
                     LDQITEVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTD
                     ITLPDGQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADN
                     RLSWQRSLPGGSQNPQQAVLPRRRTTA"
     gene            5619..6608
                     /locus_tag="OQM34_002322"
     CDS             5619..6608
                     /locus_tag="OQM34_002322"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452748.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            6708..6911
                     /locus_tag="OQM34_002323"
     CDS             6708..6911
                     /locus_tag="OQM34_002323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452749.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7013..7249
                     /locus_tag="OQM34_002324"
     CDS             7013..7249
                     /locus_tag="OQM34_002324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452750.1"
                     /translation="METHALSDDCLDCVYDWYAEKNGQFFSATSPEELLGLVAMWEVR
                     GNDWRANLNEINTYQKAIEESPFFDKNGNEIESD"
     gene            complement(7315..7547)
                     /locus_tag="OQM34_002325"
                     /pseudo
     CDS             complement(7315..7547)
                     /locus_tag="OQM34_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(7657..8427)
                     /gene="yafV"
                     /locus_tag="OQM34_002326"
     CDS             complement(7657..8427)
                     /gene="yafV"
                     /locus_tag="OQM34_002326"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1452751.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            8608..9054
                     /gene="ivy"
                     /locus_tag="OQM34_002327"
     CDS             8608..9054
                     /gene="ivy"
                     /locus_tag="OQM34_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1452752.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9097..11541)
                     /gene="fadE"
                     /locus_tag="OQM34_002328"
     CDS             complement(9097..11541)
                     /gene="fadE"
                     /locus_tag="OQM34_002328"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCS1452753.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            11781..12359
                     /gene="lpcA"
                     /locus_tag="OQM34_002329"
     CDS             11781..12359
                     /gene="lpcA"
                     /locus_tag="OQM34_002329"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1452754.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            12565..13332
                     /locus_tag="OQM34_002330"
     CDS             12565..13332
                     /locus_tag="OQM34_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1452755.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13303..14043)
                     /locus_tag="OQM34_002331"
     CDS             complement(13303..14043)
                     /locus_tag="OQM34_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1452756.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            14427..14948
                     /locus_tag="OQM34_002332"
                     /pseudo
     CDS             14427..14948
                     /locus_tag="OQM34_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15124..15621
                     /gene="rayT"
                     /locus_tag="OQM34_002333"
     CDS             15124..15621
                     /gene="rayT"
                     /locus_tag="OQM34_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCS1452757.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(15838..17550)
                     /locus_tag="OQM34_002334"
                     /pseudo
     CDS             complement(15838..17550)
                     /locus_tag="OQM34_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            17522..18307
                     /gene="lafU"
                     /locus_tag="OQM34_002335"
     CDS             17522..18307
                     /gene="lafU"
                     /locus_tag="OQM34_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS1452758.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PLKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            18378..19433
                     /gene="dinB"
                     /locus_tag="OQM34_002336"
     CDS             18378..19433
                     /gene="dinB"
                     /locus_tag="OQM34_002336"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS1452759.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            19485..19778
                     /gene="yafN"
                     /locus_tag="OQM34_002337"
     CDS             19485..19778
                     /gene="yafN"
                     /locus_tag="OQM34_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCS1452760.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            19781..20179
                     /gene="yafO"
                     /locus_tag="OQM34_002338"
     CDS             19781..20179
                     /gene="yafO"
                     /locus_tag="OQM34_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCS1452761.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            20189..20641
                     /locus_tag="OQM34_002339"
     CDS             20189..20641
                     /locus_tag="OQM34_002339"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1452762.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLTKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            20887..21930
                     /locus_tag="OQM34_002340"
     CDS             20887..21930
                     /locus_tag="OQM34_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS1452763.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            21992..22606
                     /locus_tag="OQM34_002341"
                     /pseudo
     CDS             21992..22606
                     /locus_tag="OQM34_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(22663..24120)
                     /gene="pepD"
                     /locus_tag="OQM34_002342"
     CDS             complement(22663..24120)
                     /gene="pepD"
                     /locus_tag="OQM34_002342"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1452764.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            24382..24840
                     /gene="gpt"
                     /locus_tag="OQM34_002343"
     CDS             24382..24840
                     /gene="gpt"
                     /locus_tag="OQM34_002343"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1452765.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            24932..26176
                     /gene="frsA"
                     /locus_tag="OQM34_002344"
     CDS             24932..26176
                     /gene="frsA"
                     /locus_tag="OQM34_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1452766.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            26234..26635
                     /gene="crl"
                     /locus_tag="OQM34_002345"
     CDS             26234..26635
                     /gene="crl"
                     /locus_tag="OQM34_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1452767.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(26674..>27185)
                     /locus_tag="OQM34_002346"
     CDS             complement(26674..>27185)
                     /locus_tag="OQM34_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1452768.1"
                     /translation="KQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGK
                     RAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSL
                     GYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDT
                     VAVGMTYQF"
BASE COUNT         6628 a         6876 c         7330 g         6351 t
ORIGIN      
        1 ggctagattt taatccggta ttaaaggagc aaatatcatg agttttgtat ctacgggtaa
       61 taaaccagtt ggaaacggtg gacccgtcat caccacacca ccaatagctg gcgaaagtgg
      121 cggtatgagc acaggttcag ctgtaaccga tgtatctggt gccgccgagg agatggcaga
      181 acaggctgcg gcggacctgt ttggtgcgct gccggaaccg tcaggtctgg ttaaagccgc
      241 agtagctgct gcgcaggctg ccgctgcagc agcagggatc tcggatatgg ctggtgcagt
      301 tcaggatgca gcggcgtcgc ttgcagccgg tgctccgggg gcgcataacg tcacagtaag
      361 tggtagtgcg gtgccgccgc agatgctttt gtttgcaggc atgaatggtt cggaagggct
      421 tgggaatctg ttttcttaca cagtacagct taaaacgctg gacgccctga acctggggta
      481 cgtttccccc gctgctaacc tgccgcttca gctcatggtg ggcaaagacc tgtgcgtcag
      541 tatcgaactg gacggcggcg gaaaacggta tatcagcggg ctggtgacgg cggcgagggt
      601 ggtcggccat gagggccgtt cggtaacgta tgaattacgc attgagccat ggctgaagct
      661 gctgacccat accagcgact acaaagcgtt ccagaacaaa accgtggtgg atattctcga
      721 tgaagtactg gatgaatatt cctttccggt ggaaaagcgg ctggtggaaa attatccacc
      781 ccgcgcctgg caggtgcagt atggtgaaac cgacttcgat ttcatccagc gcctgatgca
      841 ggaatggggg atttactggt ggtttgagca cagcgagaac agccacacgc tggtgctggt
      901 ggatgccatc aatggtcata aagcgtgccc tgactcgccg ctggtggagt ggcaccagga
      961 agggctgaaa ctggataagg aatttatcca caccattacg gccagtgaga ggctgcgtac
     1021 tggaaagtgg gtgatggacg attttgattt tatgaagcct cgttcgctgc ttaaaagtac
     1081 cgtggccagt ccgcgtgata ccggtcatgc tgaatacgag cattacgaat ggcctggtga
     1141 ttacttcact acgggcgaag gcgagatgct gacgcgcatt cgtatggaag cgcagcgcag
     1201 cccaggcagc cgggcacatg gggccggtca tatccgtacc ctcatgaccg ggtacacctt
     1261 tacgttgatg aatcacccga cggcagaaat taatcaggaa tatctgctgg tacagaccac
     1321 tctgttttta cgggataatg cgcagcacag cggtcaggat cagcatttta cctatgtcac
     1381 cacgtttgaa ctgcacccca ccagcgaagt ttaccgccca caacgaaccc tgagcaaacc
     1441 ccacaccaaa ggcccgcaga gcgccatcgt caccggcccg gcggggcagg aaatctggac
     1501 ggataaatat ggccgggtga aggtgcagtt tggctgggac cgttacggaa agaatgacga
     1561 aaacagttcc tgctggatcc gcgtcagcta tccgtgggcg ggtaaaggat tcggcatgat
     1621 ccagatcccg cgtattggcc aggaagtact ggtggatttc aaaaacggcg atccggattt
     1681 gcccatcatc gtggggcgca cctataacca ggacaccatg ccgccgtggg ggctgccggg
     1741 catggcgtcg cagagtggga tattcagcca ctcattgcag ggagggccga ctaacggaaa
     1801 tatgctgcgc ttcgatgata aaacgggtgc ggaagaggtg aagttccatg cggaaaaaga
     1861 cctcaatacc acggtaaaaa acaatgaaac ccacacggtc atggtcgatc gcataaaaac
     1921 cattgtcaaa aatgaaacca tcagcgtggg agaagaccgt aatgccacca tcacaaaaaa
     1981 tgatggtctt tccgtcaaac tggctcaaac catcaatgtc ggaacgacat atcgtctgga
     2041 tgttggcgat caatttaccc ttcgttgcgg caatgcagcg cttgtattac acaaggatgg
     2101 ctcgattgag ttttgcggaa aacaactgtt actgcacacc agcgatgtta tgcaactcat
     2161 tggcagtggc attgatatga atccggatgg cggcacagcc atgactgcgg atgatattgc
     2221 cccgttacct acccccgacg tttctgaata aaaaatataa aaggaatttt tctatgacca
     2281 gcctttaccg tattcaggag ggctgctttg ccctgcctga aacttttctt gatcgcaccg
     2341 tcaatatctt cgtcccatcg ggcaatgaaa gggcaactcc cagtctgaat atttcccgcg
     2401 atacgctcag gccagatgaa aatctgacca cctatattga ccgccagata gccttgatga
     2461 aaaaaaaaac ctaggccagc accgggtact ttcgcgggga acggcactgg cgggaactgg
     2521 cgaagctgcc atgacaggtg aacagattct cgcaactcat cgttctggaa aaacagaagt
     2581 gtaccagcgt caggccgggt ttatcaccgg gccagcaaag gtactaatgt tgacgctgac
     2641 cactcagcgt ccttttgatg atcacaccga ccagctatgg actgaatggt tgactagttt
     2701 tcagcctgct aaaagctaaa aaaaacaggg aggttttgtc atgtatgaag ccgcacgggt
     2761 ggatgacccc atctaccaca ccagcgccct tgccgggttt ttgatagggg ccatcatagg
     2821 tatcgccatt attgccgtcg ccagctttgc tttttttacc tgcggctttt tggccgggct
     2881 gattttgagt tttctggctg atcagatagc ttccggagtc ctgcaactgg gtgaggctat
     2941 tgggcgctcg atacattcca cagtgggaaa aattctgacc ggttctgata atgtcagcac
     3001 caacagtcgc ccggccgccc gggccgtact gagtacggtg atatgcgaag accattcccc
     3061 ggaaatccgc attgcccaag gttccggcaa catctacatc aacagccagc tgcccgtaag
     3121 gatgaccaca ctgagtgtga tgcagtgatt gaggacggct cccccaatgt ctttctcggc
     3181 ggcggtacgc agacggtact ggaaatcagc ccggaaatcc ctgactggct gcgtcaggtg
     3241 gtggatgtgc tgtatgtggt cgccggaatg cttggcgggc tggctggcgc ctggagtcag
     3301 gcggcaaaga tggggtcgaa atttggtaca aaatgtgcgg caaaatttat tggtggcgaa
     3361 atggtcggga tggccatctc tgacacagtc atgggattgt tcagcaatcc ggtagatgtc
     3421 accactgggc aaaaaatttt gctgccggag acggatttta ccctgccagg acgcctgcct
     3481 gtcacctgtt cgcgctttta cgccagccac ctggaaactg aaggattgct gggcagagga
     3541 tggcggctga actgggagat aaacctgcgt gaggatgaaa catatatcac cttcatcggt
     3601 gtacagggtc gggaactgag ttatccgaaa gagatgctga taccgggcca ccagatattc
     3661 gacccggaag agcagtttta cctgagccgt ctgcatgacg gatgctatgt gctgcattac
     3721 accgaccgca gctattacgt gtttgatgaa tttgatgacc acggcgtcgc gccgttactt
     3781 tttatggaga cgccgtaccg gcagcgtatt gctttcgggc gtgagaacgg caggctggtg
     3841 cgggtggcct ccagcagcgg acaccatctg ttattgcacc gcacgatgac gcaagcaggg
     3901 gaacgcctgt cgcatattga acttgtgaag ggcgggcgtc ccggtaacct ggtggaatat
     3961 cgttacgacg ataacgggca actgactggc gtggtgaacc gggctggtgt gacggtgcgc
     4021 cagtttgcct atgaaaatgg cctgatgaca gagcatcgca acgcgacggg ctttacctgt
     4081 acctaccact gggaagagat tgaaggtttc ccgcgcgtgg tggaacacac gaccagcgac
     4141 ggcgaggatt accggttcca ctatgatttt gctggcgggc agacggtggt aaccggcagg
     4201 ccggagcaga agtggcagtg gtggtttgat gaagagacgt atgtgactgc gcaccggacg
     4261 ccgggcggcg gaatgtaccg cttcacgtac aacgaaaacc attttccggt agcggtggaa
     4321 ctccccggcg aacgcagggt aacgctggaa tacgacaccc tgagccgggt ggtgaaagag
     4381 acggacccgg caggccgggt gacacagacg cagtggaacg gcagttttgc tgaaatcacg
     4441 cgccgggcgc tggatgacga ccacgtatgg aaggcggatt acaacgaaca cggccaggtg
     4501 atacgggaga cggacccgga agggcgcgtc acgcggtacg ggtatgatga acaggggctg
     4561 gcggtcagcc gcacggatgc gcggggcggc gaggttgcgc tggcgcacga tgcgcgcggt
     4621 caactgaggc gttacaccga ctgttcaggg tgcgccacgg gttatgaata cgatgaagac
     4681 ggcaacctga cggcggtgac ggacgcggag gggaaaacgg tacgcatcag ctataaccgt
     4741 caggggctgc cggagacggt ctgccacccc ggtaaacagc aggaccgtta cacctggaac
     4801 gcgctggggt tactgagcag ccaccgtcgc atcacgggca gtgtgcagtc ctggcagtat
     4861 acgccgcgcg ggatgctggc gcgccatacg gatgaagaga aacgggagac gcgctggcag
     4921 tacaccccgg aagggctggt tgccagtctg agcaacggca acggggctca gtaccggttc
     4981 agctacgacg gcgacggacg gctgacggga gaacagcgcc cggacggcct gataaggatg
     5041 tttgcgctga atgcggacgg gtttccggtc atcatcccga cgcagggcac agagggcggg
     5101 gtgcgcaatg aacagcagga gcgtgatgcg ctgggccgcc tgctgcgcag tgacacgcag
     5161 cacagtacac ggaccttcag ctacaaccgt cttgaccaga taacggaagt gacgctcacc
     5221 cccacggaag aaggggaacg gctgcaccac atgcaggcgg acacggtgcg ttttgcgtat
     5281 gaccgcagcg ggtggctgac ggcagagcac agcgtgcacg gcagtatcaa ataccggcgc
     5341 gatgcgctgg gcaatcccac cgatatcacg ttgccggacg ggcagcatct cagtcatctt
     5401 tactacggca gcgggcatct gctccagacg gcactggacg gtatcacggt cagtgaatat
     5461 gagcgtgaca gcctgcaccg tcaggtgata cgcacgcagg ggaaactggc gacgttcagc
     5521 ggctacaacg ccgacaaccg gttaagctgg cagcgcagcc tgccaggtgg cagccagaat
     5581 ccacagcagg cggtactccc ccggcgcaga acgacagcgt gacggcgcgg gactatatct
     5641 ggaacgcaga cggtgaagtc ggcggcatca atgacaaact gcgcggttgc ctggtgttca
     5701 gctatgaccg cagcggctgg ctgaccagcc gcacggggca gatgtatgac catgaccatt
     5761 attattacga caaagcgggc aacctgctga cggatgagta tcagggagcg gtgatggata
     5821 accgcctgcc gggttacggg cgtgaccgct accgctataa cgaatggggc gaactgacag
     5881 agcgcaggga ccagcagctg gagtggaacg cgcagggcca gttaacgcgg gtcatcagca
     5941 gcaacagcga gacgcggtat cagtacgatg cgctgggcag gcgcatcagc aaggcgacca
     6001 gcaatctcca taccgaccgg ggagaacgaa gccggacaac gtttgtgtgg gaagggttcc
     6061 ggctgttaca ggagacaacg tggcagggaa agcggacata cctgtacgat gcggaacagc
     6121 cgtacacgcc ggtggctgcc atcacggggc gcggagagag ccagaaaatc tggtattacc
     6181 acacggacct gacgggcacg gtacacgagg taaccgcgcc ggacgggacg ctggtgtggg
     6241 cgggctacca ggcgggattt ggtgaaaacc ggggcgacat cagcaacagc ggggcgtact
     6301 ttgagcagcc gcagcggctg ccggggcagt actttgacga agaaacaggg ctgcattaca
     6361 atctgttcag gtattatgcg ccggagtgcg ggaggttcgt aagccaggac ccgataggat
     6421 taaacggtgg gttgaactta tatgcttatg cgcctaatcc gttaggttgg atcgatccgc
     6481 taggactgaa ttctttagga atagattcaa ataatatatt tagaggagat ggtaactata
     6541 aaggtggcgg aattggacag ccaaagagtg gcacaatatc agcacaggac attattgatc
     6601 atctctaaga aaaaggtgat gggcgtttaa catcttttag tactaaaaaa acatctagtt
     6661 ccaactctcg tggtgtagat tttttctcaa aaaaccatgt tttcaaagtg gcagtttctg
     6721 atctcgatga tttagttaaa aataatcaga tcactgtttt aacacaagaa aaagtaaaag
     6781 caatatttgg tttagaatca agcaagaaaa ttagaaaaga acttggtaat attcttcaga
     6841 atatggctag gaataatgag gtgttagttc atgggcaaat tcttggaaat aaagttagcc
     6901 gatgtaatta gttagtggag aggaagttgt ggacgaaatt agaataagaa ttgctggtaa
     6961 tacggctgcg ccttgtttcc atgtgctaaa aaataaggga taaaaagtaa caatggaaac
     7021 tcatgcatta tctgacgatt gtttagattg tgtatatgat tggtatgcgg aaaaaaatgg
     7081 acaatttttt agtgcaacga gtccagaaga gttacttgga ttagttgcaa tgtgggaagt
     7141 tcgtggtaat gactggagag ctaatttgaa tgagataaat acttatcaaa aagccataga
     7201 agaatccccg ttttttgata aaaacggcaa tgaaatagag tctgactaaa ttttattgat
     7261 atagcaacga taataaccgg aaatttcttc cagatctttc cggcttttta ttatttaact
     7321 ttcgttcagc acactaaaca tcccgtctcg catcccctta actggctgtt taacctgata
     7381 aagttccctg cgcagttcct gtacttcgtt gttatcccaa gcaggatcaa acatccctcc
     7441 gagatcttca cggtaacgcc cgtgccgatc tcaaatcccg cttttctcag ccatttggcg
     7501 ctgattttga tcaccggcgt gtcgctgcgg ttgggcagat aacccacggt acagcgacgc
     7561 tgtgttttgg aaatggtgac ttctggcgta atattgtgtt taagttaggg gctttcgtaa
     7621 tctagcccag cccccggccc taaacacaac ccccactcac cacaacctaa actcatccgc
     7681 atcccgccat gccggaaact tttctctata ttcccgcaat gccaccatcg acagctccgc
     7741 atcaatacgc gttgccttat gcgcgtcggc ggtagcgata atctcgcctt gcggattaat
     7801 cacccggctg tcaccgcgat aatggcagcc gttgccatcg ctgccgacgc gattgcatcc
     7861 cgccacatac gcctgattct caatcgcgcg ggccgtcagc aatgcctgcc agtgcagaga
     7921 gcgcggagct ggccagttgg cgacgtacag ggcgaggtca taatcgttga gattgcgcga
     7981 ccacactgga aaacgtaagt cgtagcacac cagcggcaaa atacgccagc cgcgccattc
     8041 cacaatcact cgcgcattgc ccgctttata atgcagatgc tcatctgcca tgcggaacag
     8101 atgacgctta tcataaaaat gtaccgtgcc gcccggctca accagcaaaa agcggttaac
     8161 cgaaccagac tccgtttgta atgcaacact gcctgcaatc agcgcattgc actgctgcgc
     8221 cttagctgtc atccagttca ctacgtcatc ttgtgcaagc gacgaagctg ccgcttccat
     8281 ggcaaagccg ctggtaaaca tctccggtag aacgatcaca tcgcgcccgg taataccttc
     8341 cagttgacga tcaaaatggc gcaggttggc aggaccatcc acccacacca acggttgctg
     8401 caaaagcgta atcttcaaac taggcacggt gtacaactcc tttatgcgaa gggttttata
     8461 actttaacac cttatcaggc agttgcctta gcgcagaata aattgataac aaatgctgat
     8521 attggaaata tctgatttgc aaattatcgt gttatcgcca ggctttagga ggttaataac
     8581 atgggcagga taagctcggg aggaatgatg tttaaggcaa taacgacagt cgccgcactg
     8641 gtcatcgcca ccagtgcaat ggcgcaggat gatttaacca ttagcagcct tgcaaagggc
     8701 gaaaccacca aagctgcgtt taatcagatg gtgcaagggc ataagctacc tgcatgggtg
     8761 atgaaaggcg gtacttatac tcccgcacaa accgtaacgt tgggagatga gacgtatcag
     8821 gtgatgagcg cgtgcaaacc gcatgactgt ggctcgcaac gtatcgctgt gatgtggtcc
     8881 gagaaatcta atcagatgac ggggctgttc tcggctattg atgagaaaac gtcgcaagag
     8941 aaactcacct ggctgaatgt gaacgatgcg ctttcgattg atggtaaaac ggtgctgttc
     9001 gcggcgttga ccggcagcct ggaaaaccat ccggatggct ttaattttaa ataattagcg
     9061 gataaagaaa cggagccttt cggctccgtt attcaattac gcggcttcaa ctttccgcac
     9121 tttctccggc aactttaccg gcttcgtcgc cagctcttcc ggatcaaagt catcaacgtt
     9181 aatactgcgc agacggcttt cttccgcttt caccagaata gcggcttcat ctttatcaat
     9241 cagccccttc gtcagcgcgt tgtgcgccag ttcatccaga cgggtaaatg gcagattttt
     9301 acccagctct ttacagatcc gctgatgaat cgggtcggcg gcaatcacat ccaccaacgc
     9361 ctcttccagt aaaccaaccg ggttatgctc gcttggcgtc aggtactgac cgcgaccaat
     9421 acgtgaacga gtggcgttcg gcacttgtaa aatcttcgcc actttatgat ccagcttgtc
     9481 agacggtgcc agataatgac gtccggtcgg gaagatcacc acattcagca gcccggcaac
     9541 cacgcggttc gggaagtttt gcagtaaatt gtccattgcc tgttctgcct gatacagcgc
     9601 atcctgtacg ccccagtgca ccagcggcag atcggcttca ttacggcctt cgtcgtcata
     9661 acgcttcaga acggcagagg cgaggtaaag ctggcttaaa atatccccca gacgtgccga
     9721 gatacgctca cgacgtttca ggctgccgcc cagcagtgcc atcgagacat cagaaagcag
     9781 ggcgaggttg gcgctcaggc ggttcaggtg ctgatagtag cgtttagtgg catcgccggt
     9841 tggcgtgctg ctggttaaac cgcgcgtcag gcccagccag aagctgcgaa ctttgttgct
     9901 accgacgtga ccgatatgtt tgaacaacag tttatcgaac gcgttgacgt cattgttctt
     9961 cgccgcttcc atctcttcca gcacgtacgg atggcaacga atcgctcctt gtccgaagat
    10021 catcatgctg cgggtcagaa tgttagcccc ttcaacggtg atggcaatcg gtgcgccctg
    10081 gtaagcacgc gccaggaagt tgctttgccc gagcataatg cctttaccgc cggtaatatc
    10141 catcgcatca ataatcgact gctgcccgcg gtgggtacag tgatacttaa cgatagccga
    10201 cagcacggca ggtttttcgc cgagcataat gccgtaggta atcagcgatg ccgcagcatc
    10261 cattacataa gcgttcccgg caatacgcgc cagcggctct tcaatccctt ccatcttacc
    10321 gatagagatt ttgaactgac ggcgaatgtg agaatacgcg cccgttgcca gcgctaccga
    10381 tttcacgccg ccggttgagt tggaaggcag ggtgatgccg cggcctaccg agaggcactc
    10441 caccagcatc cgccagcctt gcccggccat tttcggcccg ccgatgatgt aatcgatcgg
    10501 cacgaagaca tctttaccgc gcgtcggtcc gttctggaac ggtacgttca gcgggaagtg
    10561 gcgacgacca atttccacgc ccggcgtggt ggttgggatc agcgcacagg taatgcctaa
    10621 atcttctgca ccgccgagta atttttccgg gtcggagagt ttaaacgcca gcccaagcac
    10681 ggtcgcaatc ggtgccagcg taatgtagcg tttgttccag gtcagacgca tccccagcac
    10741 ctgctggccc tgccattcgc ccatgcagac aatcccggtg tccggaatcg cgcccgcatc
    10801 ggaacccgct tccgggctgg tcagtgcaaa gcaggggatc tcctgaccac gcgccagacg
    10861 cggcagatag tgatctttct gctcgtcagt gccgtaatgt tgcaacagtt cgcccgggcc
    10921 taatgagttt ggcacgccga cggtaatcgc caggatcccg ctcacgccag agagtttttg
    10981 cagcacacga gactgggcat aagctgagaa ctccagtccg ccgtactctt ttttgataat
    11041 catcgcgaag aagcgatgct ctttaaggta cgcccacaac tccggcggca gatccgccag
    11101 ctcatgagta atctggaaat cattcgccat ccggcaggct tcttctaccg ggccgtcgag
    11161 aaacgcttgc tcttcagcgg tcaggcgcgg ctgcggatag ttatgcagct ttttccagtc
    11221 cggcttgccc tggaacaagt cgccctccca ccaggtggtg cccgcatcaa tcgcttcttt
    11281 ctcagtgcgc gacatcggcg gcatcacctt acggaaaccg cgaaataccg gcgcggaaat
    11341 catcgactta cgcataggcg caaagttaaa tggcacgagg ataatggtca gaggcaccag
    11401 tacccacgcc gaccacagac cagcaacgcc gagggcggct gtccaggcga gcaaaatcag
    11461 actgctgata aataagctca cgcggtgata gaacaacgcg ccgagcagga caaccgtagc
    11521 gagaatactc aaaatcatca taacgaaaag ccccttactt gtaggaggtc tgaccacttg
    11581 tgatgatatg gttgtagtgg atgtaaaaac atttagcaat gtgtttacaa tataattaca
    11641 acaaagctca cattgttgct gtttttatcc gcacttcagg tcaaaaagtc ctggtcatag
    11701 cacctgcccg tacttctcgc ttttggcggt atccggtaca ctgcattttg tctattacat
    11761 ttatgctgaa ggatatcctc atgtaccagg atcttattcg taacgaactg aacgaagcgg
    11821 cggaaacgct ggctaacttt ttaaaagatg acgccaatat tcacgccatt cagcgcgcgg
    11881 cggtcctgtt agcagacagc tttaaagccg gtggcaaagt gctttcctgc ggcaacggcg
    11941 gttcccattg cgacgctatg cactttgccg aagagttgac cggtcgctac cgtgaaaacc
    12001 gtccgggcta cccggcgatt gctatttctg acgttagtca tatttcctgc gtcggtaatg
    12061 atttcggttt caatgatatt ttctcccgct acgttgaagc ggtaggtcgc gaaggcgatg
    12121 tactgctggg gatctccacc tccggtaact ctgcaaacgt gatcaaagcg atcgcagcgg
    12181 cgcgtgagaa ggggatgaaa gtgatcaccc tgaccggtaa agacggcggt aaaatggctg
    12241 gcacggcgga tatcgaaatt cgcgttccgc actttggtta tgccgaccgc attcaggaga
    12301 ttcacattaa agtgatccat atcctgatcc agttaattga aaaagagatg gttaagtaag
    12361 tctggcgtag gccggataag gcgtttacgc cgcatccggc acttgtgcgc tgatgcctga
    12421 tgcgatgctg acgcgtctta tcatgcctac aaatctgtac ccgaaccgta ggccgaataa
    12481 ttcgtttacg ccgcatccga cctgaaaatt cttaaatcaa tcttcgccgg ggtccatgcg
    12541 ctcccgctgt tgtggaggtt acccatgtgc gaattgctcg ggatgagcgc caacgtccct
    12601 accgatatct gctttagttt caccggactt gtacagcgtg gtggtggaac cgggccacat
    12661 aaagatggct ggggcattac cttttacgaa ggtaaaggct gtcgcacatt taaagatcca
    12721 caacccagct ttaattcccc catcgccaaa cttgtccagg actacccgat aaaatcctgt
    12781 tcggtggtgg ctcatattcg ccaggctaat cggggcgagg tggcgctgga aaatactcac
    12841 ccatttaccc gcgagttatg ggggcgtaac tggacttatg cccataacgg acaactgacg
    12901 ggctacaaat cactggaaac cggcaacttc cgcccggtcg gtgaaaccga cagcgaaaaa
    12961 gccttttgct ggctgctgca taaattaacg cagcgttacc cgcgcacgcc gggcaacatg
    13021 gcggcagtgt ttaaatatat cgcctcactg gcggatgaac tgcgggcgaa gggcgttttc
    13081 aacatgctgc tttcggacgg gcgctatgta atggcgtatt gctcgactaa tttacactgg
    13141 atcacccgcc gcgcgccgtt tggcgtggca acgttgctgg atcaggatgt ggaaatcgac
    13201 ttcagctcgc agaccacacc gaatgatgtg gtcacggtga ttgcgacaca gccgctgacg
    13261 ggcaatgaaa cctggcaaaa aattatgcca ggcgaatggc gcttattttg cctcggggag
    13321 cgtgtagttt gatgccagtt gtggctgcac cacttcgtgg cttaacggct tgctgaccac
    13381 gtaacggcca ttggccacag aaacggtcgg tggcttacgg gtttgctcaa agtagtcgta
    13441 gcccggcttc agctgctccc agaagtcctt aaagttggaa tatttatggc gcttcatatt
    13501 ggcgtcggtc atgcggaatg ggtaaatact cacctgcacg ctcggctgac caaacaccag
    13561 cgcaccagta acgaactgga agatctcatc aataccctga ttggtcattg cgtagcagcc
    13621 gatggataca caatcgccgt ggatcatcag gtatttccct tcataaccat gcgcacggtc
    13681 ataagcattg gggaatccaa tattaatcgc tttgtagtaa cggctgtctg gttttaactg
    13741 attacgctgg acgctataaa acccttccgg gcttttgaaa tcgccctgac gctgttttgg
    13801 ccctaagccg ccggaatatt tacagatttt ataactgtcg agcagttgat attgctcgcc
    13861 cattttgacg taaagatcga gcgtacgttc ttccttgaag atctggatgt agacagggga
    13921 tcccatcaac tgctgcttat actctttgct cactggcgtg gtcgagctac tgctgcccag
    13981 caaaccggca aacgaaacgc acgggatcaa caacatcgca agaattaatg cgattttacg
    14041 catactgctt attccttgat aaaacggtta cacacgccag gacggcaaaa tggatcccaa
    14101 atcggaatag tctggatttg gaaggctcac attatcacca aaagagtttt acgcaagcct
    14161 gtcggcgctg ggtttacaaa tttagcacag gggcagtttt caatagtccg aaaagcgcat
    14221 gtatcattta tttcctaata ttatgaaatc tcaatagtaa tatgcaaata agcaattaat
    14281 aatcccgttt actcctccgg tcttttaaaa ttctcacctt cattcagccg gtttatgtct
    14341 ttcatgtctt cgttcttgct cggcagattt ttgcatccag gcgtgttttc gttatgcgtt
    14401 ttgctccctt tactcgccag cgccaccaca accgggagca gataaaacag catccagaat
    14461 ggtttcctgc gccgctcaag gcgagtgaca gacgctggca ggcgctggcg gaaaacaacc
    14521 actttttaag cagcgatcac ctgcataaca tcaccgaagt ggcgattcac cgcctggagc
    14581 agcagcttgg caagccttac gtctggggcg gcacgcggcc cgatcaaggc ttcgattgta
    14641 gtgggttagt tttttatgcc tacaacaaga tccttgaggc gaaactcccg cgcacggcca
    14701 atgagatgta ccactaccgt cgggcaacga ttgtggcgaa taacgacctg cgccggggag
    14761 atttgctatt tttccatatc cacagccgcg agattgccga ccatatgggg gtgtatctgg
    14821 gcgacggtca gtttattgag tcactgcgca ccggggaaac cattcgggta agccggttag
    14881 ccgaaccttt ctggaaggac cattttttag gcgcgcgcag gattttgacg gaagagatga
    14941 ttttgtaggc cggataaggc gtttacgccg catccggcgg ttgtgcacgg atgcctgatg
    15001 cgacgctgac gcgtcttatc atgcctacaa atctgtgcgt ttttgcgagc cgctttcccg
    15061 atataacaca gcgtcattgt cggcctttcc ttttcctctt accgttgaga aaaaggagtc
    15121 gctatgtcgg aatatcgtcg ttattacatc aaggggggaa catggttttt cacggtaaat
    15181 ttacgcaatc gtcgaagcca tcttctgacc acccagttcc agacgctccg taacgccatt
    15241 attaacgtta agcgagacag accttttgaa attaacgcct gggtcgtttt gccggagcat
    15301 atgcattgca tatggacatt acccgaaagc gatgatgatt tttcctctcg ctggcgggag
    15361 attaaaaagc agttcaccca tgcttgcgga ttgaaaaata tctggcaacc gcgtttttgg
    15421 gagcatgcca ttcgcaatac aaaagattat cgccatcatg ttgattatat ttatataaat
    15481 ccagagaaac atggctgggt aaagcaggtg agtgattggc cattctcaac gttccatcgc
    15541 gatgtcgcgc gagggttata tcccatcgat tgggcgggag acataacgga tttaagtgcc
    15601 ggggagcgta tcattttata actgtgcgct ggtgccggat gcgcttacgc ttatccggcc
    15661 tacaaatcgt gctttaacgg taggccggat aaggcgttca cgccgcatcc ggcagttatg
    15721 cgcagatgcc tgatgcgacg ctggcgcgtc ttatcatgcc taaaaacttg tgcctgaacg
    15781 gtaggccgga taaggcgttt acgccgcatc cggcaattgt gcaccgatgc cggattctta
    15841 catcaacgcc cccataatct tcaactccag ctcatccggc acttcgttat acgacagcac
    15901 atgcagcccc ggcgcaaaca accttgcata acgcgccagc aaagggcgta gctgcggcgg
    15961 caccagcagc accggatctt tccccgccgc tttcatctgc tctttcacct gtggcatcgt
    16021 gctctggaac tggttgagca tattcgggtc aaccggcacg ctgtcgagca tcactttgcc
    16081 ggcctgttgc gcctgattca ctacgttggt cagcagattt tccagctcat tattcagcgt
    16141 atacaccgtc agctcctgct tgcgaacgaa cggatgagta atgctgcgcc gcagcgccag
    16201 gcgcacatcg gccgccagca gaatatgatc tttagtcacc gtgctactgg cgaccagcac
    16261 ggtggcgatg gtgacgatat cgcgcaggga aacgccttcg gtcagcagcg cccggtacac
    16321 tttcagcaat tggctgtaat tgagcgccgc gcttaaatcc tccgccaggc gcggtgccat
    16381 cgacgacaaa cggttatgca actgcgtgat gtcatcatag ttaaacaaat caggaatata
    16441 gctgcgcaca atcttgttca catgggtggc aatcacactg gcgctgtcga tcacctgata
    16501 ccccatattc agagccttcg ctttctgcgc aggctggatc caggtgaccg gtatcccgta
    16561 cgccgggtcg ttccccagca cgccgtcaat ctcgccgtag gtttcgctgg agggcagcgc
    16621 catcagttta tccgccggaa tatccgcttc atcagcctca atgccgttga tgaaaatagc
    16681 gtactggctg ggcttgaggc ggaagttttc ccgaatgcgg atctccggca gcagcacgcc
    16741 gttaccgtcg gaaatcacct gccgaacgcc gcgaatccgc tgggtgagcg ggttgccctg
    16801 cgctttgtcc accagcgcca ccagcttgta gccgaggctt aagctgatgg gctcgatcag
    16861 cggaatagtt tcccaactga cctgttgttc gctggtttca gtgatggtgc gggtcagcgt
    16921 ttcgaggctt ttctcttccg cctccgccgc ctgcgggcgt ttgctcatcc gccagccggt
    16981 aaagccaagc agggcgctga acagcaaaaa cggcagatgc ggcatccccg gcaccaccgc
    17041 cagcacgaac atgatcccgg tggcggtgta gagcaccgac gggcttgcca gcaactggtg
    17101 gcgcacgtca tgggcgatat cgccgttgtc gctgacgcgg gtgacgataa tcgccgccgc
    17161 ggtggagagc agcagggaag ggatctgcgc caccaggccg tcgccgatgg tcatcagcac
    17221 gtattgctgg aaggcggcgt cggcgctcag gttgtatttg aagatgccga tacagacgcc
    17281 gccgctcagg ttgatcgcca gaatcatcat cccggcgatg gcgtctccgc gtacaaactt
    17341 cgatgccccg tccatcgcgc cgtagaaatc ggcctcgctg gcaacatctt tacgccgggt
    17401 ttgcgcctgc gcctggttga tcaatccggc gttaagatcg gcgtcaatcg ccatctgttt
    17461 gccgggcatc gcgtccaggg taaagcgggc agaaacctcg gaaatacgct cggcctcttt
    17521 ggtgacgacg ataaagttga ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat
    17581 cgccgcgctg cacgggcagt cgatttttaa cggtggtggg ctgtcgccgt taaataaaat
    17641 cagcccgtct catccgctaa aacctgccac cgtggcggtg ccggaagaga cggagaaaaa
    17701 agcgcgtgat gtgaatgaga aaacggcgct gctgaagaag aaatccgcca ccgagcttgg
    17761 cgagttggca accagtatca acaccattgc ccgtgatgcg catatggaag cgaatctgga
    17821 gatggagatt gttccccagg gattacgcgt gctgattaaa gacgaccaga atcgcaatat
    17881 gttcgaacgc ggcagcgcgc agattatgcc gttctttaaa actttgctgg tggagctcgc
    17941 gccagtgttc gactcgctct ataacaaaat tattattacc gggcataccg atgcgatggc
    18001 ctacaaaaac aatatctaca acaactggaa cctttcgggc gatcgcgcgc tttcagctcg
    18061 tcgggtgctg gaagaggccg gaatgccgga aaataaagtg atgcaggtaa gcgcaatggc
    18121 ggaccagatg ctgctggacg cgaagaatcc gcaaagcgcg ggcaatcggc gtattgagat
    18181 tatggtgctg accaaaagtg cgtccgatac gctgtatcaa tactttggtc agcatgggga
    18241 taaagtggtg cagccgctgg tgcaaaagct ggataagcag caggtgcttt cgcagcgaat
    18301 gcgctaaatg ctgaatcttt acgcatttct caaaacctga aatcactgta tactttacca
    18361 gtattgagag gtgagcaatg cgtaaaatca ttcatgtgga tatggactgc tttttcgcag
    18421 cggtggagat gcgcgacaat cccgctctgc gcgatatccc tattgctatt ggcggcagcc
    18481 gcgaacgtcg gggggtgatc agcaccgcca attatcccgc gcgtaaattt ggcgtacgta
    18541 gcgctatgcc tactgggatg gcgctcaaat tatgcccaca tctcaccttg cttccggggc
    18601 gctttgacgc ctacaaagaa gcctcaaatc atatccgtga aatcttctcg cgctacacct
    18661 cgcgcattga accgttgtca ctggatgagg cttatctcga tgtcaccgat agcgtccatt
    18721 gccacggttc tgcgaccctc atcgcccagg aaatccgcca gacgatcttc aacgagctgc
    18781 aactgacggc atctgcgggc gtggcaccag taaagtttct cgccaaaatc gcctccgaca
    18841 tgaataaacc caacggtcag tttgtgatta cgccggcaga agttccggca tttttacaaa
    18901 ccttaccact ggaaaaaatc cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga
    18961 tggggctgcg gacctgcggt gatgtacaaa agtgtgatct ggtgattctg cttaaacgct
    19021 ttggcaaatt tggccgcatt ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta
    19081 acagcgaacg gttacgaaaa tccgtcggcg tggaacgcac gatggcggaa gatattcatc
    19141 actggtctga atgtgaagcg attatcgagc ggctgtatcc ggaacttgaa cgccgtctgg
    19201 caaaggtaaa acctgattta ctgattgctc gccagggggt gaaattaaag ttcgacgatt
    19261 ttcagcagac cacccaggag cacgtctggc cgcggctgaa taaagctgac ttaatcgcca
    19321 ccgcgcgtaa aacctgggat gaacgccgcg gcgggcgcgg tgtgcgtctg gtggggctgc
    19381 atgtgacgtt gcttgacccg caaatggaaa gacaactggt gctgggatta tgatgtatac
    19441 tattatgtat attctggtgt gcattattat gagggtatca ctgtatgcat cgaattctcg
    19501 ctgaaaaatc ggtcaatatc actgagttac gtaaaaaccc agctaaatac tttattgatc
    19561 aaccagttgc ggttctttct aataatcgcc ccgcaggata tctcttaagt gccagcgcat
    19621 tcgaagcgtt aatggacatg cttgctgaac aagaggagaa aaagcccata aaggcgcgct
    19681 ttcgtccaag tgctgcaaga ttagaggaaa ttacacgccg cgctaaacaa tatcttaatg
    19741 atatgacgga tgatgatttc aatgacttta aggaataagg atgcgggtat tcaaaacaaa
    19801 acttattcgc ctgcaactta cagcagagga acttgatgcg ttaacggcgg attttatttc
    19861 ctataagcgt gacggtgttt tgccagatat atttggtcgc gatgcactct acgacgactc
    19921 gtttacctgg ccattaatca aatttgagcg agttgctcat attcatctgg caaatgtgaa
    19981 taatccattt ccgccacagt tgcgccaatt cagcagaacg aatgacgaag cgcatttggt
    20041 atattgtcag ggtgcgtttg atgagcaagc atggttgctc attgccattc tgaagcctga
    20101 acctcataaa ctggctcgag ataacaacca aatgcataaa attggaaaaa tggcagaagc
    20161 gtttcgcatg cgtttttgaa tttatattat gaataacata caaataagag attatcagcc
    20221 tggcgatttt cagcaactat gcgctatttt cattagagcg gttacgatga ccgtcagtca
    20281 gcattattca ctacaacaaa ttgccgcctg ggcgcagatt gacgaatctc gctggaagga
    20341 gaaactcacg aaatcacaag tgcgggttgc agttattaat gcaaaactgg tcggctttat
    20401 tacctgcgtt gaacactata tcgatatgtt atttgttgac cctgaataca cccgccgtgg
    20461 ggttgccagc gctttgttaa aaccttttat taagtctgaa tccgaactta cggtggacgc
    20521 aagcataacc gcaaaaccct tttttgaacg ttatggtttt cagacagtta agcagcagcg
    20581 cgttgaatgc cggggagcgt ggtttattaa tttttatatg cgttataaac cgcaacatta
    20641 aatccagctt gcaatgaaaa taacgcccgc ctagtatgtg cgcgttttct tattccacaa
    20701 actgcaagga ggtaaatcat gacaaatcct ttatcaatga ctctttgcag acctttccag
    20761 gattaattct ttttttcttg ccctggattc gtctgccatt tcctgatttt ttaattgtta
    20821 ctctcaataa ttcaagtcgc agcacatgca acttgaagta tgacgagtct atttacggaa
    20881 tggtatatgg gcaattatat tcgtccctta tccgatgcgg tatttaccat cgcatctgat
    20941 gacctgtgga tcgagagttt agcgatccaa caattacaca ccacggcaaa tttacccaac
    21001 atgcagcgcg tagtggggat gccagattta caccccggac gcggctaccc gattggcgca
    21061 gcgttcttct ccgcaggccg tttttaccca gcactggtcg gcaatgatat cggctgtggc
    21121 atggcgctat ggcaaacaga tattctcgct cgcaaataca acgccgataa gtttgaaaag
    21181 cgattatcag ctttggatga cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca
    21241 gcatttgcac agcatccgtg gcgcagctcg cttggctcta tcggtggtgg caatcatttc
    21301 gctgaactgc aacaagttga tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg
    21361 gatgcgcagc atctgcaact gctggttcat agcggctcga gggggctagg gcagtctatt
    21421 ttacagcggc atattgcctc gttttcgcat catggtttgc ctgaaggcag tgacgacgcg
    21481 ctggcgtttg cgcgttttaa tcgccacctg atcgctttgc gcataatgca acaggttaag
    21541 gccaccggaa gtccggttct ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt
    21601 gatcaacaag gggtcttaca tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt
    21661 attcccggtt cacgcggtga ttactccagg ctggttcagc ccgtcaggag tgaggaaaca
    21721 ttgcattcgc tggcgcatgg ggctgggcgt aaatgggggc gcaccgagtg taaagggcgt
    21781 ctggcagcga aatacacagc gacgcaactc tcgcgtactg aacttggcag tcgggtaatt
    21841 tgtcgcgata aacaactcat cttcgaagaa gcgccacaag cctataaatc ggctgaaagt
    21901 gtggtgcaat gtctgggcag gccggattaa ttattcctgt cgcgcggctg cgtccggtgc
    21961 taacgctcaa aaacagtgga gggaaaaaag gatgatcttg ctataactct cctcagcgca
    22021 ggggccggaa gaatgttgtc tcgcagtgaa aaaggcgctg gacaggctaa ttaaagaagc
    22081 tgcccgacaa gacgtcgcgg taacggtgct ggaaacagaa acaggccgct actctgacac
    22141 gctgcgttcg gcgctgattt ctctggatgg cgacaacgca tgggcgttaa gcgaaagctg
    22201 gtgtggcact attcagtgga tttgcccgag cccgtatcgc ccacatcacg ggcgtaaaaa
    22261 ctggtttctg ggcattgggc gttttaccgc tgatgagcag gagcaatccg atgcgatccg
    22321 gtatgagacg ctgcgttcgt cggggccggg cggtcaacat gtcaataaaa ccgactcggc
    22381 ggtacgcgcc acgcatttgg catccggtat tagcgtgaag gttcagtcag agcgcagcca
    22441 gcatgctaac aaacggctgg cgcgattgct gattgcctgg aagctggatc agcagcaata
    22501 ggaaaatagc gcggtgctga aatcacagcg gcgaatgttc catcaccaga ttgaacgtgg
    22561 caacccgcga cgaacgttta cagggatggc ttttatcgaa ggataatgaa ggatgaaact
    22621 gccggaaggc gattaaacgc catccggcag caaatcaaat aattacttcg ccggaatttc
    22681 tttcagcagt tcagtcagca gtgtccagta atgacctacg ctttcgatgt gaacttgctc
    22741 atccggagag tgtggaccgg tgatagttgg cccgatagaa accatgtcca tttccggata
    22801 cggttttttg aacagaccac attccaggcc cgcgtggata atctggatgt tcggcgtctt
    22861 gttgaacagg cgctgatagg tttcacgtac cagatgcatc accggagaat tagcgtccgg
    22921 ctgccagcca ggatatgcgc ctttcgcttc ggttttcgcg ccagccagtt tacccagcga
    22981 atccagcatg ctcaccacgt agtctttacc gctgtcgatc agtgaacgga tcaggcagtg
    23041 aatttctacg ttattgtcag tcatggtcac cacaccgacg ttcagggagg tttcgaccac
    23101 accttttgcc acgtcggaat tgcggatcac accgttcggg gtggcgttca gcagacgaat
    23161 aaaggtatcg cgagatttcg caatcagggc agctttatcg ttcgctacag agtccagcaa
    23221 caaggccaga tttttctctt tctctgccag ctcgtttttc aggatctcct gataggtatt
    23281 caccagagat ttcagtgcgt cgactttatc agctgcgaca gcaatggtcg caaaggcttc
    23341 acgcgggatg gcgttacgca gtgtgccgcc gttgaaatcg ataaggcgca ggtccagttc
    23401 ttctgcatga cccgccagga agcgcaccag cagtttgttg gcattaccca ggccaacgtg
    23461 gatttcaccg ccggagtgac cgcctttcag accttttaag gttaacttga aggtttcaaa
    23521 accagctgga accgcttcac gatctaaatg caggttggag gtgaagtcga taccccccgc
    23581 acaacccatg tagatttcac cttcttcttc ggagtcggtg ttaatcagaa tatcagcctg
    23641 caaccagttg ctctgtaagc cgaacgcacc gtccataccg gcttcttcgg tcatggtcag
    23701 cagcacttcc agcgggccgt gaaccacgtt ttcgtcagcc agaaccgcca gtgcagaggc
    23761 cataccaatg ccgttatccg cacccagcgt ggtgccgcgc gctttaaccc attcgccatc
    23821 aatataaggc tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat ttttctgcgg
    23881 caccatatcg aggtgggcct gtaagacgac cggtttacga ttttccatac ctgcggtagc
    23941 aggtttacga atcaggatat tacctacctg atcgcgttcg acatggaaac ctttctcttt
    24001 tgcccaacca acaatgtatt cagcgagttg ctcttcatga taggacgggt gaggaataga
    24061 acagattttg gcaaaaatat cccacagcgg ctgtggagat aattgagaca gttcagacac
    24121 gttaagtctc cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg ttagcagaaa
    24181 atgttgtcaa cacaagacag gcttgcgaga tatgtattga gaataccact ttatcccgcg
    24241 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
    24301 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
    24361 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
    24421 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
    24481 gccgtaagcc gtggcggtct ggtaccgggg gcgttactgg cgcgtgaact gggtattcgt
    24541 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg
    24601 ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc
    24661 ggtggtactg ctgttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
    24721 gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc
    24781 tggatcgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
    24841 tcttttcaac gcctggcatt gccgggcgtt gttcttttta acttcaggca ggttacaata
    24901 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
    24961 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
    25021 cgcacaaccg cctgtgcagt cggctcttga tggtaaaacc atccctcact ggtatcgcat
    25081 gattaaccgt ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca
    25141 ggcacgtatt gtgatgagcg atgctgaacg taccgacgat gatttatacg ataccgtgat
    25201 tggctaccgt ggtggcaact ggatttatga gtgggccacc caggcgatgg tgtggcaaca
    25261 aaaagcctgt acggaagaag atccgcaact cagtggtcga cactggctgc atgcggctac
    25321 gttgtacaac attgccgcct atcctcatct gaaaggagat gacctggccg agcaagcgca
    25381 ggctttatca aaccgcgcct atgaagaggc cgcccagcgt ctgccgggca cgatgcggca
    25441 gatggagttt accgtacccg gcggtgcgcc catcaccggc tttttgcata tgccgaaagg
    25501 cgatggcccg ttcccgacag tattaatgtg tggtggtctg gatgcgatgc agacggacta
    25561 ttacagcctg tatgaacgtt attttgcgcc gcgcggcatt gcgatgctga ctattgatat
    25621 gccgtcggtg ggcttttctt caaaatggaa gctcacccag gactccagcc tgttgcatca
    25681 gcacgtctta aaggcgctgc ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt
    25741 tggtttccgt ttcggcgcta acgttgccgt gcgtctggca taccttgaat cgccgcgtct
    25801 gaaagcggtt gcctgtcttg gtccggtagt tcataccctg ttgagtgatt ttaagtgcca
    25861 gcaacaggtg ccggaaatgt atcttgacgt tctggcgagt cgtttgggga tgcatgatgc
    25921 ttccgacgaa gcgttacgcg tggagctgaa tcgctattca ttaaaagtgc aaggattgct
    25981 ggggcgtcgc tgcccaacgc caatgttatc aggctactgg aagaacgatc cgttcagccc
    26041 ggaagaggac tcacgcttaa tcacctcatc atctgctgac ggtaaattat tagagatccc
    26101 atttaacccg gtgtatcgga attttgacaa aggtcttcag gaaatcaccg gctggatcga
    26161 aaaacgcttg tgttaaaaat ttgctaaatt ttgctaattt ggtaaaacag ttgcatcaca
    26221 acaggagata gcaatgacgt taccgagtgg acacccgaag agcagattga tcaaaaaatt
    26281 taccgcacta ggcccgtata ttcgtgaagg taagtgcgaa gataatcgat tctttttcga
    26341 ttgtctggct gtatgcgtca acgtgaaacc ggcaccggaa gtgcgtgaat tctggggctg
    26401 gtggatggag ctggaagcgc aggaatcccg ttttacctac agctaccagt ttggtctgtt
    26461 cgataaagca ggcgactgga agagtgttcc ggtaaaagat actgaagtgg ttgaacgact
    26521 ggagcacacc ctgcgtgagt ttcacgagaa gctgcgtgaa ctgctgacga cgctgaatct
    26581 gaagctggaa ccggcgaatg attttcgtga tgaaccggtg aagttaacgg cgtgagtgaa
    26641 atgtgccgga tgcatcacat ccggcaatat tcattaaaac tgatacgtca tcccaaccgc
    26701 gacagtatca tcattattaa tattcagctt attatcgcta tccagttggt tgattttata
    26761 atcaacaaac gctgacatat ttttgttgaa ataatacgta gcaccgacgt cgatataatt
    26821 gaccagatct tcatcaccga tgccctcaat atctttccct ttcgataaga cataacccag
    26881 cgatggacgc agaccaaagt caaactggta ttgagcgacc gcttcaaagt tctgtgtctt
    26941 attggcaaag ccgccagtta ttggcgtcat tttgcgtgtt tcagaataga aagttgccag
    27001 ataaatatta ttggcatcgt atttcagacc tgttgcccat gcttctgcac gcttgcctgt
    27061 gccacggctt tgcaggttct gctcgttggt gcgatctgag ttggtatagg ccccactaat
    27121 ggcgaaatcg ctgccgccaa agtcatatgt caatgacgtg ccgaagccat cgccgttttg
    27181 ctttt
//
LOCUS       DAJVPP010000057        26135 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVPP010000057 DAJVPP010000000
VERSION     DAJVPP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.057"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..221)
                     /locus_tag="OQM34_002347"
     CDS             complement(<1..221)
                     /locus_tag="OQM34_002347"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1452769.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKKLARLQRQLSR"
     gene            complement(354..659)
                     /locus_tag="OQM34_002348"
                     /pseudo
     CDS             complement(354..659)
                     /locus_tag="OQM34_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            727..1342
                     /gene="tnpA"
                     /locus_tag="OQM34_002349"
                     /pseudo
     CDS             727..1342
                     /gene="tnpA"
                     /locus_tag="OQM34_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1670..2752
                     /locus_tag="OQM34_002350"
     CDS             1670..2752
                     /locus_tag="OQM34_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCS1452770.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3241..4039)
                     /locus_tag="OQM34_002351"
                     /pseudo
     CDS             complement(3241..4039)
                     /locus_tag="OQM34_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4049..4600)
                     /gene="ybcL"
                     /locus_tag="OQM34_002352"
     CDS             complement(4049..4600)
                     /gene="ybcL"
                     /locus_tag="OQM34_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCS1452771.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4769..5101)
                     /gene="emrE"
                     /locus_tag="OQM34_002353"
     CDS             complement(4769..5101)
                     /gene="emrE"
                     /locus_tag="OQM34_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCS1452772.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5446..5760)
                     /gene="fliE"
                     /locus_tag="OQM34_002354"
     CDS             complement(5446..5760)
                     /gene="fliE"
                     /locus_tag="OQM34_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1452773.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5974..7639
                     /gene="fliF"
                     /locus_tag="OQM34_002355"
                     /pseudo
     CDS             5974..7639
                     /gene="fliF"
                     /locus_tag="OQM34_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7632..8627
                     /gene="fliG"
                     /locus_tag="OQM34_002356"
                     /pseudo
     CDS             7632..8627
                     /gene="fliG"
                     /locus_tag="OQM34_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
     gene            8620..9306
                     /gene="fliH"
                     /locus_tag="OQM34_002357"
     CDS             8620..9306
                     /gene="fliH"
                     /locus_tag="OQM34_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1452774.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9306..10679
                     /gene="fliI"
                     /locus_tag="OQM34_002358"
     CDS             9306..10679
                     /gene="fliI"
                     /locus_tag="OQM34_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCS1452775.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10701..11042
                     /gene="fliJ"
                     /locus_tag="OQM34_002359"
                     /pseudo
     CDS             10701..11042
                     /gene="fliJ"
                     /locus_tag="OQM34_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11039..12166
                     /gene="fliK"
                     /locus_tag="OQM34_002360"
     CDS             11039..12166
                     /gene="fliK"
                     /locus_tag="OQM34_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1452776.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSVPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12271..12735
                     /gene="fliL"
                     /locus_tag="OQM34_002361"
     CDS             12271..12735
                     /gene="fliL"
                     /locus_tag="OQM34_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1452777.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12740..13745
                     /gene="fliM"
                     /locus_tag="OQM34_002362"
                     /pseudo
     CDS             12740..13745
                     /gene="fliM"
                     /locus_tag="OQM34_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
     gene            13742..14155
                     /gene="fliN"
                     /locus_tag="OQM34_002363"
     CDS             13742..14155
                     /gene="fliN"
                     /locus_tag="OQM34_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1452778.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14158..14523
                     /gene="fliO"
                     /locus_tag="OQM34_002364"
     CDS             14158..14523
                     /gene="fliO"
                     /locus_tag="OQM34_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1452779.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14523..15260
                     /gene="fliP"
                     /locus_tag="OQM34_002365"
     CDS             14523..15260
                     /gene="fliP"
                     /locus_tag="OQM34_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCS1452780.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15270..15539
                     /gene="fliQ"
                     /locus_tag="OQM34_002366"
     CDS             15270..15539
                     /gene="fliQ"
                     /locus_tag="OQM34_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1452781.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15548..16333
                     /gene="fliR"
                     /locus_tag="OQM34_002367"
     CDS             15548..16333
                     /gene="fliR"
                     /locus_tag="OQM34_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS1452782.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16618..17241
                     /gene="rcsA"
                     /locus_tag="OQM34_002368"
     CDS             16618..17241
                     /gene="rcsA"
                     /locus_tag="OQM34_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1452783.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17285..17473)
                     /gene="dsrB"
                     /locus_tag="OQM34_002369"
     CDS             complement(17285..17473)
                     /gene="dsrB"
                     /locus_tag="OQM34_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1452784.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17636..17863
                     /gene="yodD"
                     /locus_tag="OQM34_002370"
     CDS             17636..17863
                     /gene="yodD"
                     /locus_tag="OQM34_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCS1452785.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18161..18976
                     /locus_tag="OQM34_002371"
                     /pseudo
     CDS             18161..18976
                     /locus_tag="OQM34_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18973..20667)
                     /gene="yedQ"
                     /locus_tag="OQM34_002372"
                     /pseudo
     CDS             complement(18973..20667)
                     /gene="yedQ"
                     /locus_tag="OQM34_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20838..21020)
                     /locus_tag="OQM34_002373"
     CDS             complement(20838..21020)
                     /locus_tag="OQM34_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1452786.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21099..22016)
                     /locus_tag="OQM34_002374"
     CDS             complement(21099..22016)
                     /locus_tag="OQM34_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1452787.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22189..23109
                     /gene="yedA"
                     /locus_tag="OQM34_002375"
     CDS             22189..23109
                     /gene="yedA"
                     /locus_tag="OQM34_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCS1452788.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTSKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23098..23568)
                     /gene="vsr"
                     /locus_tag="OQM34_002376"
     CDS             complement(23098..23568)
                     /gene="vsr"
                     /locus_tag="OQM34_002376"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1452789.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23549..24967)
                     /gene="dcm"
                     /locus_tag="OQM34_002377"
     CDS             complement(23549..24967)
                     /gene="dcm"
                     /locus_tag="OQM34_002377"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1452790.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(25034..25574)
                     /locus_tag="OQM34_002378"
                     /pseudo
     CDS             complement(25034..25574)
                     /locus_tag="OQM34_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25614..25871)
                     /locus_tag="OQM34_002379"
     CDS             complement(25614..25871)
                     /locus_tag="OQM34_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452791.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            complement(25872..>26135)
                     /locus_tag="OQM34_002380"
     CDS             complement(25872..>26135)
                     /locus_tag="OQM34_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1452792.1"
                     /translation="SFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTR
                     EEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT         6592 a         6699 c         6417 g         6427 t
ORIGIN      
        1 cggcttaact gtcgctgaag tctcgccagc ttcttctggt ttttctgaaa actgtttaca
       61 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
      121 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
      181 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
      241 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgagcg
      301 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
      361 aacgccgagc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
      421 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
      481 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
      541 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
      601 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
      661 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
      721 ttcctgatgc atgctcaccg ggtctttgtt accaagtacc gacgccagat ttttgaccat
      781 gacgcgacag aaaaactacg cacttacttt tcaaatgtat gtgcttattt tgaagctgaa
      841 ctggttgaaa tggatggcga accagatcac gtccatctgt taattaacta tccccccaaa
      901 ctggcgatat ccagtctggt aaacaaacag cctcaaaggc gtatcgggta ggttactgcg
      961 acgggatcga ccagatattg ctgccaggta ttaccgcaaa gatgttttgt ggcgtcccgg
     1021 ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca
     1081 acagcaaaca cctggtcaga tgaaaaaacg agccttatat ccccgccctg aaggacgggc
     1141 ttttacggtg cactggataa gcctggagat tatctacacc tgcatggaca tcattgtact
     1201 atgccccgta catgccccat cacatcctca acatcaaagt attagagcct tgcaaaccat
     1261 tatgcgtctc agtttgtctc atctatctta cgcaaaaaaa atagaactaa tatcaagcgg
     1321 cgctatattg cgtattaaat aaaaccacct caattacatt aataaatcta aataaatatc
     1381 atgcaaataa tttccatggt tattttcagg ctatttaaat taaaaaaata aattatgagt
     1441 caatcataaa taacaacctt taatataatt tgactaccaa atttatattc gttcacaaaa
     1501 tagtcatgca ttgtgtgcaa tagaaacagt tcagataaaa atagggatct acttcacaat
     1561 ttaaatgaga aactaaaact tacatcttga aataatctca ttgattggat gaatatttgt
     1621 agtgcagtga catcattttt aaataatagt tcaaataaaa gggctcacga tgaaaaaatt
     1681 aacagtggca atttctgctg tagctgcatc agtactgatg gcgatgtctg ctcaggcagc
     1741 agagatttac aataaagaca gtaacaagct ggatctgtac ggaaaagtta atgctaagca
     1801 ctacttctct tctaacgatg cagatgatgg cgacacaact tatgttcgtc tgggcttcaa
     1861 aggcgaaact caaatcaacg atcaactgac tggtttcggt cagtgggaat atgaattcaa
     1921 aggcaaccgt gctgaatctc aaggttcttc caaagataaa acccgtcttg ccttcgctgg
     1981 cctgaaattc ggtgactacg gctccatcga ttatggccgt aactacggtg tagcatacga
     2041 tatcggtgca tggaccgacg ttctgccaga attcggtggc gatacctgga cccaaacaga
     2101 tgtgttcatg actggtcgca ccacaggtgt tgcaacctat cgtaacaacg acttctttgg
     2161 tctggttgat ggtctgaact ttgctgctca gtatcagggt aaaaatgacc gcactgacgt
     2221 aactgaagct aacggtgatg gtttcggttt ctccactact tatgagtatg aaggattcgg
     2281 tgtaggtgca acctatgcta aatctgaccg cactaatgat caggttatct acggtaacaa
     2341 cagcctgaat gcatctggtc aaaatgctga agtatgggca gctggtctga aatatgatgc
     2401 gaacaacatc tatctggcta ccacctattc tgaaacccag aacatgactg tttttggtaa
     2461 taaccatatt gccaacaaag cacaaaactt cgaagtagtt gcacaatatc agttcgactt
     2521 cggtctgcgt ccgtccgttg cttacctgca atctaaagga aaagacttgg gtgcgtgggg
     2581 tgatcaggac ctgattgaat atattgatgt aggtgcaacc tattacttca acaaaaatat
     2641 gtccactttt gttgattaca aaatcaacct gattgataag agcgatttca cgaaagcatc
     2701 tggcgttgct accgatgata tcgttgctgt aggattggtt taccagttct aatctgatta
     2761 ctcaaagata tgttgtggga ggctttgcct ccccaacata taagtggcgc tctcaagcca
     2821 ctttccttag aagcactatc ttgcttctga ctatataaat taccctttat tttctgggac
     2881 gtttcaacgc cccattttta ataatactca gtgaacaatt aacgtatcaa ttagatttaa
     2941 taacaacgat atccgtacct aacctgatat ctaatgtcat aagcatccct ttaattatcc
     3001 cctcaacctc ttgtaaattt cccactcttg agctccacat agcccaaacg ccggatgcaa
     3061 aaaaaatcac acgtgcatct ctgaaattgt gatggaggaa gtgagtatca attatatgtg
     3121 cttataaacg ttcccgattg gcacagaagt caccggattg ttcaggctcc aataggttga
     3181 gtatgtcgta tttattttcc aaaataaaaa tcgatgctgt tgctccttaa taaaatactt
     3241 ttactctctg gacgcttttc ttagctctct tggtgttata ttaaaacgat tatggaacct
     3301 ttcagtaaaa cgagaaggac acttgtaacc attttccctg gcaatctcgc tgatgggttt
     3361 tactgtcgtt tgtatagcag gacaacgcat tatttaacct cacgtcgtcc aatatacttt
     3421 gaaaacttat tccctcgctt gccagacggc gatgcaatgt ggaaacagaa atgtagagat
     3481 atctcgctac cttgtttgct gtccattttg ttctaggttc ggatagcatt aggttataac
     3541 aacgacttat taatgattgt ttactatctg ataaaagtaa acaattaaca tgatgtgctc
     3601 ctaatgaaag tagaacgccc attgctaagt gttcctgaat ttgcgttgag aagcctcggg
     3661 aaatagatgt ttttagttgc tcccaacaat atattaactc ggggttctga ggtaagcaga
     3721 aacctgtcgt gttacgaatt tgatcagtta tcgcataaaa tttttgaaac ttttcaatta
     3781 gatcaatggg ataataaagc atttctgcaa gataaagacc tgcttctgga taattcacaa
     3841 tataaaattc atatccacag ggaaataata ttatctgatt attatcaaca gttagactat
     3901 gcgtctccca attgataact ttttttccct gacggatacg acataaagct ggcataagag
     3961 gcttaaccct gtgaatctca tgatgtttat gcatccgtat ttcttcaatc tttaggttag
     4021 acttatctct tgccagcata ctcttaccct attttatctc ataaactggt gttatctcag
     4081 cggttgcaat tttattggca ttaagcatat aaccaactaa cgctccgctg gaattagaat
     4141 ccacaggaat cttatcagtt tttagagccc atactttaaa ctggtaatga tggggtttat
     4201 ctcctttggg aggacatgct ccaccaaatc ctgcataacc aaaatcattt cggccttgaa
     4261 cagcaccagt cggaagtttt gtaccatcac gtcttcctgc atcagtgggc aaatatgtta
     4321 cagttgctgg aatattagca acagtccagt gccaccatcc actgcctgta ggagcatctg
     4381 gatcatacac agttacggca aagcttttgg tgccttcagg agcaccagac caggttaatg
     4441 agggcgatgt attaccacct tcacacccaa atccagaaaa tacatgagac gttgtaagtt
     4501 gctctcctgt ttttatttca ttactagtga cctgaaatgc tgcggcctgc gcagaaaatg
     4561 ttatgaatgc caatacactt gaaacgataa gttttttcat aaaaacctca ttgttgtgac
     4621 ctatcgttat tttatttgat gtgccttgat ctcattatga ataaaggcgc aatgatcgcg
     4681 caaatgcaat cataattata aaggtgcccc gaacattagc cataatatgc acaaatcaga
     4741 ttcctgttat ctcaaggaga attatttttt aatgcggtgc acttcgtgac aacaagttaa
     4801 tgactaacac accggcacaa atcaacatca tgcctatgat agctggcagg tccagtcgtt
     4861 gaccaaaaaa tccccaggaa agcaaactaa tcagaacgat accgactcct gaccagatag
     4921 cataagcaat ccctgtaggt atataagcca gcgtctgagc taataaccag aatgatgcac
     4981 aatagcaaat aattgtgcca acagagggcc ataaccgtgt aaacccctct gaaaacttca
     5041 ttaaggttgt accaatgacc tctgcaagta ttgcgccacc aagataaata taagggttca
     5101 tagcatattc tttcctgttc atactggaga gaattgtact acagtttgaa cacaactcac
     5161 cgttttcatc ctggtgtatg tgtatccaag gggctctggc gtggcaagag ataaaggcag
     5221 gaacaataat gatcagaagg aaccccaggc taacgaatga gttcactctt cctgggagca
     5281 aagcgaagac aaaacatgtt atcgcgttta cggatcgtgg atggcagaaa acaaccagga
     5341 tcaggtatcc attttaaacc agaaattgag tgagtttgcc ccatccatgc cccacgctgt
     5401 gggatcggat ataattaaac aagcatagtc aacaggttac acaacctaca cctgcatgga
     5461 catcacttcc tgatacgccg ccaccagctt attacgcacc tgaatcccca tttgcataga
     5521 aactgaggct ttttgcatat cggtcatcac atcgtttagc gccacgccgg gttcaccgag
     5581 ggtgaatttt tctgcctgcg tgcgggcgac tgtttgtgta tcgcttatgc gatcgagcgc
     5641 ggcgtgcagc tgcccggcaa aactaatggt cggttgcggc agtgattcct gcgcacgcgc
     5701 actcatcgcc gtagcctgta actggctgat aaccccttca atcccctgta tcgctgacat
     5761 tttcgtctcc cggataattt ctggtaacaa agcctaccag taagtcaata agacaaaggc
     5821 gctaaatagc aacaaaaaaa ctggtttatt ggcggataga aaaaacgaaa gcacaaataa
     5881 tgggagcgtc aatttttcga gtttgctgac ccgagagtga gtcttgttcc actttgccaa
     5941 taacgccgtc cataatcagc cacgaggtgc gcgatgaatg cgactgcagc ccagacaaaa
     6001 tctcttgagt ggcttaatcg cctgcgtgcg aatccgaaaa ttccattgat tgttaccggt
     6061 tccgcggcag tggcggtcat ggtcgcactg atcctgtggg cgaaagcccc cgactaccgc
     6121 acattattca gcaatctttc cgatcaggat ggtggcgcaa ttgtcagcca actgacgcaa
     6181 atgaatattc cttaccgctt cagcgaagcc agcggcgcta ttgaggttcc ggcagataaa
     6241 gttcacgaac tgcacctgcg cctggcgcaa caaggtttgc ccaaaggcgg cgcggtcggt
     6301 ttcgaactgc tcgatcagga aaagtttggt atcagccagt tcagcgaaca ggtgaattat
     6361 cagcgggcgc tggaaggcga gctttctcgt accatcgaaa ctatcggccc ggtaaaaggg
     6421 gcgcgcgtac atctggcaat gccgaaaccg tctttattcg tccgtgaaca aaaatcccct
     6481 tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac tcgatgaagg gcaaattagc
     6541 gctattgtac atctggtttc cagcgcggtt gctggtctgc cgcctggaaa cgtcacgctg
     6601 gtggatcagg gcgggcatct gttaacccag tccaatacta gcgggcgcga tcttaatgac
     6661 gctcagttga aatatgccag cgatgtcgaa ggccgtattc agcagcgtat tgaagcgatc
     6721 ctgtcaccta ttgttggtaa cggtaatatt cacgcctagg tcacggcgca actggacttc
     6781 gccagtaaag aacaaacgga agaacagtat cgccctaacg gtgatgaatc tcatgcggcg
     6841 cttcgttcac gccagcttaa tgagagcgag caaagcggtt ccggttatcc gggcggcgta
     6901 ccgggggcgt tgtcgaatca accggcacct gcgaataacg cgccaatcag cacgcctccg
     6961 acaaatcaaa ataaccgcca gcagcaggcg agcaccacca gcaatagtgg gccgcgtagc
     7021 acacagcgga atgaaaccag taactacgaa gtcgatcgca ccattcgtca taccaaaatg
     7081 aacgtgggcg atgtgcaacg tctgtcagtc gcggtagtgg tgaattacaa aaccttgcca
     7141 gacggtaaac cattgcctct cagcaacgaa cagatgaagc aaattgaagc tctgacccgc
     7201 gaggcgatgg gcttttctga aaaacgcggt gattcgctca atgttgttaa ctcgccgttc
     7261 aatagcagtg acgaaagcgg cggagcactg ccgttttggc aacagcaagt gtttatcgat
     7321 cagttgctgg ctgccggtcg ctggttgctg gttgctggta ctgctggtgg cgtggttgct
     7381 gtggcggaaa gcggtacgcc cgcagctaac acgtcgcgcc gaggcggtga agactgtgca
     7441 gcaacaggcg caggcccacg aggaagtgga agatgcggtg gaagtccgtc tgagcaaaga
     7501 cgaacaactc caacaacggc gcgctaacca acgactgggg gcagaagtca tgagccagcg
     7561 tatccgtgaa atgtctgata acgatccgcg cgtggtggcg ctggtcattc gccagtggat
     7621 aaataacgat catgagtaac ctgacaggca ccgataaaag cgtcatcctg ctgatgacca
     7681 ttggcgaaga ccgggcggca gaggtgttca agcacctctc ccagcgcgaa gtgcaaaccc
     7741 tgagcgctgc aatggcgaac gtcacgcaga tctccaacaa gcagctaacc gatgtgctgg
     7801 cggagtttga gcaagaagct gaacagtttg ctgcactgaa tatcaacgcc aacgattatc
     7861 tgcgttcggt attggtcaaa gctctgggtg aagaacgtgc cgccagcctg ctggaagata
     7921 ttctcgaaac ccgcgatacc gccagcggta ttgaaacgct caactttatg gagccgcaga
     7981 gcgccgccga tctgattcgc gatgagcatc cgcaaattat cgccaccatt ctggtgcatc
     8041 tgaagcgcgc ctaggccgcc gatattctgg cgttgttcga tgaacgtctg cgccacgacg
     8101 tgatgttgcg tatcgccacc tttggcggcg tgcagccagc cgcgctggcc gaactgaccg
     8161 aagtactgaa tggcttgctc gacggtcaga atctcaagcg cagcaaaatg ggcggcgtga
     8221 gaacggcagc cgaaattatc aacctgatga aaacgcagca ggaagaagcc gttattaccg
     8281 ccgtgcgtga attcgacggc gagctggcgc agaaaatcat cgacgagatg ttcctgttcg
     8341 agaatctggt ggatgtcgat gatcgcagca ttcagcgtct gttgcaggaa gtggattccg
     8401 aatcgctgct gatcgcgctg aaaggggccg agcagccact gcgcgagaaa ttcctgcgca
     8461 atatgtcgca gcgtgccgcc gatattctgc gcgacgatct cgccaaccgt ggtccggtgc
     8521 gtctgtcgca ggtcgaaaac gaacagaaag cgattctgct gattgtgcgc cgtcttgccg
     8581 aaactggcga gatggtgatt ggcagcggcg aggataccta tgtctgataa tctgccgtgg
     8641 aaaacctgga cgccggacga tctcgcgcca ccaccggcag agtttgtgcc aatggtcgag
     8701 tcggaagaaa ccattattga agaggccgaa cccagccttg agcagcaact ggcgcaactg
     8761 caaatgcagg cccacgagca gggttatcag gcggggattg ccgaaggtcg ccagcaaggt
     8821 cacgaacagg gctatcagga aggactggcc caggggctgg aacaaggtct ggcagaggcg
     8881 aaggctcaac aagcgccaat tcatgcccgg atgcagcaac tggtcagcga atttcaaact
     8941 acccttgatg cgcttgatag tgtgatagct tcgcgcctga tgcagatggc gctggaggca
     9001 gcacgtcagg tgatcggtca gacaccaacg gtggataact cggcactgat caaacagatc
     9061 caacagttgt tgcagcaaga accgttattc agcgggaaac cacagctgcg cgtacacccg
     9121 gatgatctgc aacgtgtgga tgatatgctc ggcgcaactt taagtttgca tggctggcgc
     9181 ttgcggggcg atcccaccct ccaccctggc ggatgtaaag tctccgccga tgaaggcgat
     9241 ctcgacgcca gtgtcgccac tcgctggcaa gaactctgcc gtctggcagc accaggagtg
     9301 gtgtaatgac cacgcgcctg actcgctggc taaccacgct ggataacttt gaagccaaaa
     9361 tggcgcagtt gcctgcggta cgtcgctacg ggcgattaac ccgcgctacc gggctggtgc
     9421 tggaagccac cggattacaa ttgccgctcg gcgcaacctg tgtcattgag cgccagaacg
     9481 gcagcgaaac gcacgaagta gaaagcgaag tcgttggctt taacggtcaa cggctgtttt
     9541 taatgccgct ggaggaagtg gaaggtgtcc tgcccggcgc gcgtgtatat gccaaaaaca
     9601 tttcggcaga agggctgcaa agcggcaagc agttgccgct cggtccggcg ttattaggtc
     9661 gtgttctgga cggtagcggt aaaccgctcg atggcctgcc cttccccgat acgacggaaa
     9721 ccggtgcgct gattaccccg ccatttaacc cgttgcaacg tacaccgatt gaacatgtgc
     9781 tggacaccgg cgtgcgccca atcaatgccc tgcttaccgt cggacgcggg cagcgtatgg
     9841 ggctgtttgc cgggtcgggc gttggtaaaa gtgtgctgct ggggatgatg gcacgctaca
     9901 cccgcgccga tgtcattgtc gtgggtttga ttggtgaacg tgggcgcgaa gtaaaagatt
     9961 ttattgagaa tatcctcggt gccgaaggac gtgcacgctc agtggtgatt gccgctccgg
    10021 cggatgtttc tccgctcctg cgaatgcaag gtgccgccta tgccacgcgc attgccgaag
    10081 attttcgcga tcgtggtcag catgtgttgc tgattatgga ctccctcacc cgctacgcga
    10141 tggcccagcg tgaaattgcg ctggcgattg gcgaaccacc tgccactaaa ggttatccac
    10201 cgtcggtgtt tgccaaatta ccggcactgg tcgagcgtgc cggtaatggc attagcggcg
    10261 gcggctcgat taccgcgttt tataccgtgc tcaccgaagg cgatgaccag caggacccca
    10321 ttgctgactc cgcgcgggca atcctcgacg gtcacattgt gctgtctcgc cgactggcgg
    10381 aagccgggca ctatccggct atcgatattg aagcgtcgat cagtcgcgca atgacggcgt
    10441 tgatcagtga gcaacattac gcgcgagtgc gcaccttcaa acagctgttg tcgagttttc
    10501 agcgtaaccg cgatctggtt agcgtcggcg cgtatgccaa aggcagcgat ccgatgctcg
    10561 ataaagccat cgccctgtgg ccgcagctgg agggctattt gcaacaaggc atttttgaac
    10621 gcgcggactg ggaagcgtct ctccaggggc tggagcgtat tttcccgaca gtgtcataac
    10681 ccaggagata acggcagatg gcggaagaac agctcaaaat gttgatcgat tatcagaatg
    10741 aatatcgcaa taacctcaat agcgatatga gtgccgggat gaccagcaac cgctggatca
    10801 actatcagca gtttatccag acgctggaaa aagccattac tcagcatcgc cagcaactta
    10861 atcagtggac gcagaaagtt gacattgccc tgaacagctg gcgagaaaag aaacaacgtt
    10921 tgcaggcctg gcagacactg caggaacggc aatccacggc ggcactgctt gcagaaaacc
    10981 gcctcgatca gaaaaagatg gatgagttcg cccagcgcgc cgccatgagg aaacctgaat
    11041 gattcgctta gcgcccttga ttaccgccga cgttgacacc accacattgc ctggcggcaa
    11101 agccagcgat gctgcacaag attttctcac gttgttgagc gaagcattag caggcgagac
    11161 aacaactgac aaagcggccc cccagttgct ggtagcaaca gataagccca cgacaaaagg
    11221 cgagccgctg gtcagcgata ttcttgccga tgcgcaacaa gcggatttac tgatccctgt
    11281 ggatgaaaca cttcctgtca tcaacgacga acaatccaca tcaacaccgt taaccaccgc
    11341 tcagacgatg acgttggctg cggtggctga caaaaatacg acaaaagacg aaaaagcgga
    11401 tgatcttaat gaagacgtca ccgcaagtct gagcgccctt tttgcgatgt tgccgggttt
    11461 tgacaatacg cccaaagtga ctgatgcacc gtcaaccgtg ttaccggcag agaaaccaac
    11521 gctcttcaca aaactgactt ctgcgcaact cactacagca cagcctgatg acgcccccgg
    11581 cacaccagct cagccattaa caccgctggt agcagaagcc cagagtaaag cggaagtcat
    11641 cagcacgcct tcgccggtga ccgctgacgc cagcccgcta atcactccac accagacaca
    11701 gccactgccc accgtcgccg cgcctgtttt gagtgtaccg ctgggttctc acgaatggca
    11761 acaatcattg agccagcata tttcgctgtt cacccgccag gggcaacaaa gtgcagagtt
    11821 gcgtctgcac ccacaggatt taggtgaagt gcaaatttcc ctcaaagtgg atgataacca
    11881 ggcgcagatc cagatgattt caccgcatca gcacgtacgc gccgccctgg aagcagcgct
    11941 gccggtactg cgtacgcagc tggccgaaag tggcattcag ttagggcaaa gcaacattag
    12001 tggcgaaagc tttagtggtc agcagcaggc cgcttcccag caacagcaaa gccaacgcac
    12061 agcaaaccat gaacctctgg cgggggaaga cgacgatacg cttccggttc ccgtctcttt
    12121 acaagggcgt gtaacaggta acagcggcgt tgatattttc gcctaacgtc agaggtagca
    12181 ccgtaatccg cgtcttttcc ccgctttgtt gcgctcaaga cgcaggataa ttagccgata
    12241 agcagtagcg acacaggaag accgcaacac atgactgatt acgcgataag caagaaaagc
    12301 aagcgatcgc tttggatccc gattctggta ttcattaccc tcgcggcctg tgccagcgca
    12361 ggttacagct actggcattc gcatcaggtt gccgctgacg acaaagcgca gcaacgcgtc
    12421 gtgccctccc cggtcttcta tgcgctggat acctttacgg tcaatttggg cgatgcggat
    12481 cgcgtgcttt atatcggcat aaccctgcga ctgaaagatg aagcaacccg ctcgcggctg
    12541 agtgagtatt tgccggaagt ccgtagtcgc ttgctgttac tgttttcacg tcaggatgct
    12601 gccgtactgg cgacagaaga aggcaagaaa aacctgattg ccgagattaa aaccacactt
    12661 tccaccccgc ttgttgccgg gcaaccgaaa caggatgtca ccgacgtgct gtataccgct
    12721 tttattctgc gataacgaca tgggcgatag tattctttct caagctgaaa ttgatgcgct
    12781 gttgaatggt gacagcgaag tcaaagacga accgacagcc agtgttagcg gcgaaagtga
    12841 cattcgtccg tacgatccga atacccaacg acgggttgtg cgcgaacgtt tgcaggcgct
    12901 ggaaatcatt aatgagcgct ttgcccgcca ttttcgtatg gggctgttca acctgctgcg
    12961 ccgtagcccg gacattaccg tcggggccat ccgcattcag ccgtaccatg aatttgcccg
    13021 caacctgccg gtgccgacca acctgaacct aatccacctg aaaccgctac gcggcacagg
    13081 gctggtagtg ttctcgccga gtctggtgtt tatcgccgtg gataacctgt ttggcggcga
    13141 tggacgcttc ccgaccaaag tggaaggtcg cgagtttacc catactgaac agcgcgtcat
    13201 caaccgtatg ttgaagctgg cgctggaagg ctatagcgac gcctggaaag cgattaatcc
    13261 gctggaagtt gagtacgtgc gttcggaaat gcaggtggaa tttaccaata tcaccacctc
    13321 gccgaacgac attgtggtta acacgccgtt ccatgtggag attggcaacc tgaccggcga
    13381 atttaatatc tgcctgccat tcagcatgat cgagccgcta cgggaattgt tggttaaccc
    13441 gccgctggaa aactcgcgta atgaagatca gaactggcgc gataacctgg tgcgccaggt
    13501 gcagcattca cagctggaac tggtggccaa ctttgccgat atctcgctac gcctgtcgca
    13561 gattttaaaa ctgaaacccg gcgacgtcct gccgatagaa aaacccgatc gcatcatcgc
    13621 ccatgttgac ggcgtcccgg ttctgaccag tcagtactgg tactctcaac ggtcagtatg
    13681 cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag gaacagccca
    13741 aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg atctgtgggc
    13801 tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc gctgccgacg cggtgttcca
    13861 gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga ttatggatat
    13921 tccggtcaag ctgaccgtcg agctgggccg tacgcggatg accatcaaag agctgttgcg
    13981 tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc tggatattct
    14041 gatcaacggt tatttaatcg cccagggcga agtggtggtt gttgccgata aatatggcgt
    14101 gcggatcacc gatatcatta ccccgtccga gcggatgcgc cgcctgagcc gttagtgatg
    14161 aataaccatg ctactgtgca atcttccgcg ccggtttctg ctgagccact gctgcaggtg
    14221 agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct ggtaaaacgg
    14281 ctgggatttg cccctaaacg cactggcgtt aacggtctga aaattagcgc cagtgcttcg
    14341 ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag atgcacggct ggtgctcggc
    14401 gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc aacggaagag
    14461 ataccgcaga ccgattttca gtcggtcatg aaaaatttgc ttaagcgtgg cgggagatcc
    14521 tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg gctgattacg cccctcgcct
    14581 tcgcgcaact gccgggtatc accagccagc cgctacctgg cggtggacaa agctggtcgc
    14641 tcccggtgca gacgctggtg ttcatcacct cgttgacgtt cattccagcg attttactga
    14701 tgatgaccag tttcacccgc atcattattg tttttggttt attgcgtaac gcgctgggaa
    14761 cgccctccgc gccacctaac caggtattgc tggggctggc actgtttttg acctttttta
    14821 ttatgtcgcc agtgatcaac aaaatttatg tcgatgcgta ccagccattc agcgaagaga
    14881 aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag tttatgctgc
    14941 gtcagacccg cgaggcagat ttagggttgt ttgccagact ggcgaatacc ggcccgttgc
    15001 aggggcctga agctgtgccg atgcgcattt tgctcccggc ctacgtgacc agcgagttga
    15061 aaaccgcatt tcagataggc ttcacgattt tcatcccttt tttgattatc gacctggtga
    15121 ttgccagcgt gttgatggca ttggggatga tgatggttcc ccccgccacc attgctctgc
    15181 cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc ggttcgctgg
    15241 cgcagagctt ttacagctag agaggcaaaa tgacacctga atcggtcatg atgatgggaa
    15301 ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct gttgctggta gcgctggtca
    15361 cgggccttat catcagtatt ttgcaggcag ccacgcagat taacgaaatg acgctgtcgt
    15421 ttattccgaa aatcatcgcc gtatttatcg ccatcattat tgccggaccg tggatgctca
    15481 atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc atcgggtagc
    15541 ccgtacaatg atgcaggaga caagcgatca atggctttcc tggttaagcc tgtacttctg
    15601 gccgttactg cgcgtgctgg cgctcatttc taccgcgcca attctgagcg aacgcagcgt
    15661 gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgccattg ccccatcatt
    15721 acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg tacagcagat
    15781 cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg tgcgaaccgc
    15841 tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acatttgtcg atcctggcag
    15901 ccatctcaat atgcccgttt tagcgcgcat catggatatg ctggcattac tgctgttcct
    15961 gacatttaac ggtcatttat ggttgatttc actgctggtc gatacctttc acaccctgcc
    16021 gattggtagc gaaccgttga acagcaatgc gtttctggca cccaccaaag cagggagttt
    16081 gattttcctt aacgggctga tgctggcgtt accgctcatt actctgttgc tgaccctgaa
    16141 tctggcatta ggtttactga atcgtatggc cccgcaatta tccatttttg ttattggatt
    16201 tccattaact ctgactgtcg gcatctcttt aatggcggca ttaatgccgt taattgcgcc
    16261 tttttgcgaa catttattca gtgaaatttt taatttgctg gctgatatta ttagtgaatt
    16321 accattaata taattccgta acgtttatca tgctacccta aggattatcc gaaaaataat
    16381 atctgcgaac atcttccagg atactcctgt agcgaaatat ttgttttaag ctcactcaca
    16441 tatcgcaaca tttactttaa gacaattcca ggcaaattat acaacacttt acgggatagt
    16501 aagtccgcct gaaaaattgc gagagtggcg cattaggtga cccatgttgt tccgtttagt
    16561 catgatgaaa tattcaggta aggggaatta tcgttacgca ttgagtgagg gtatgccatg
    16621 tcaacgatta ttatggattt atgtagttac acccgactag gtttaaccgg gtatctgttg
    16681 agtagagggg ttaaaaaaag agaaatcaac gacattgaaa ccgttgatga ccttgccata
    16741 gcttgtgatt cacagcgtcc ttcagtggtg tttattaatg aggactgttt catccacgat
    16801 gcttctaaca gtcagcgcat caagcacatc attaatcaac atcccaatac gttatttatc
    16861 gtttttatgg caattgccaa tgttcatttt gatgaatatc tattggtcag aaaaaattta
    16921 ttgatcagtt ctaaatcgat taaaccggaa tctctcgacg atatccttgg cgatattctg
    16981 aaaaaagaga caacgataac ctcgttttta aatatgccga cgttatcatt gagccgaacc
    17041 gaatcgagta tgttgcgaat gtggatggca ggtcagggaa ccattcaaat ctccgaccaa
    17101 atgaatatca aagccaagac cgtttcatcg cataaaggta atattaaacg taagatcaaa
    17161 acgcataata aacaggttat ctaccatgtc gtccgactga cggataatgt gactaatggt
    17221 atttttgtca acatgcgcta acacattctg actggtggtt tcccaccagt caggctgaat
    17281 aagattactc tgctttctca acaaagatac cgtcctgatg ccctgcttca ttaaagaacc
    17341 agatgccgag cgggtagtct tccagcgcaa ccaggtacat tgtgccttca ctaaactcct
    17401 caactgccag taccactcca ggacgacgcg gaccgccatc cgttttgact gttacccgat
    17461 cattcacctt catcgttttc ctcctgtggc tttgtgccag tgtagaacaa tttcgttttt
    17521 ctggcagcgc caagcgcgcg cgagtgctga ttttctcgac ggtctatact taagagatgc
    17581 caggcggact taacgactgg cggcaacaac agagtaacgg ttgcgaggaa agatgatgaa
    17641 aaccgcaaaa gtgtacagcg ataccgcaaa acgtgaggtc agcgtcgatg tcgatgccct
    17701 gctggcggcg atcaatgaaa ttagcgaaag cgaagttcat cgcagccaga acgattctga
    17761 acacgttagc gtcgatggac gtgaatatca tacatggcgt gaattggcgg atgccttcga
    17821 actggatatt catgacttca gcgtctctga agtgaatcgt tgaatgcaca ataaaaaaat
    17881 cccgaccctg agggtgtcgg gatgaaactt gcttaagcaa gaagcactta aaaaattcgt
    17941 tacaccagga aatctgatgt gttcatcacc ttatctgcaa tttttttcgc tgacaagaaa
    18001 atattcgcgc tatgaatgat taacgctact tatgaataga aatgtgacgc gcgtcacccc
    18061 ttggtggctc tggtgtcaac gggatcgctc ccgaaaaaat catctttgat tatttgtgct
    18121 gttttgagaa tcatcctggt aaggggagta ctgcgcgacc atgttttcaa ttcaacaacc
    18181 actactggtt tttagcgatc ttgatggcac cctgctggac agtcatagtt atgactggca
    18241 accggcagcc ccctggctca gccgtttaca cgaagccaat attcccgtca ttctctgtag
    18301 cagtaaaact ttagcggaaa tgctgtactt gcaaaaaatg ttggggctac aaggcttacc
    18361 gctgattgca gagaatggcg cagtgatcca gcttgctgaa caatggcagg atatagacgg
    18421 ttttccacgc atcatctcag gtattagcca tggcgaaatc tgccaggttt taaatacgct
    18481 acgcgagaaa gaacatttta aattcacgac ttttgatgat gtcgacgatg caaccattgc
    18541 cgaatggacg ggattaagcc gtagctaggc ggcgctgacg cagctgcatg aggcgtcggt
    18601 aacgctaatc tggcgcgaca gtgacgagcg tatggcacaa tttatcgctc gtctgaacga
    18661 actgggcttg cagtttatgc agggtgcgcg cttctggcac gtactggatg cctctgccgg
    18721 aaaagatcag gctgccaact ggattatcgc gacctatcaa caattgtcag gcagacgccc
    18781 aaccacactt ggcctgggcg atgggccaaa cgatgcgcct ttactggagg taatggatta
    18841 cgcggtgatt gtgaaagggc taaaccgtga aggggtacat ctgcatgatg aggatccggc
    18901 ccgcgtctgg cgaacgcagc gtgaaggacc ggaaggctgg cgtgaagggc tggaccattt
    18961 tttctccgcc cgttaagcgt tatcgctcgc gaatacccga ttgcgcccgg cctgttttgc
    19021 gaggtaaagc cgacggtcgg ccaatgactg gagttgttca aaatcataat caccggtttc
    19081 ctcgctgcta cttaccccca gcgaggcact gatgcgtatc gttgtactct tggcgatcag
    19141 catctctttt tcatttaact tcaggcgaat acgttctgcg acctccgcag cctgcgtcag
    19201 attcgcgcct ggcagaatca cacaaaactc ctcaccaccg acccgcccgg caacgtcctg
    19261 cgcacgcaag gaactgctaa ttaatccggc agcatgagaa agaacacggt cgcccgcctg
    19321 atgaccaaag cggtcattaa tcgctttaaa atggtcaaga tcgacctgaa tgacagaaaa
    19381 aggatgttgg tgcgtctgac acaatttagc gagcggacgg gctttttcga acagtgcgcc
    19441 acggttatat aaacgcgtta aggtgtcgtg ccacgcctgc cactgcaacg agctttgcag
    19501 aacatacatg ttgctgacca tccggcgaat cacataccag gagaggagta acatggtggt
    19561 aaagagcgcc cacagcaggg ttaatgcaat gctgatactg ccgaaatcgc cgcgcacgcc
    19621 ttcgcttagc gtatggacgc gcgccagcac accgtcgaaa tggtccagac gttcccagct
    19681 aacatagcga ctgtccatac gaatgccgcc ccgcgtgtca tgttccattg cctgcgccag
    19741 caaggccagt tcacgaggat caaaaatatt ccctgttgga tggtcaggat tggaagaggt
    19801 caaaaatctc agcttactgt catagagctg atactcacca tcgaggtttt tatcgatggc
    19861 gtttcttaaa aattgctgca tagtacgcac gggaatactc atccccagca cgccatacca
    19921 gtagttatta ctgtctaccg gaacactgac ggtaacctgc ggttcagtat tgctggcgtg
    19981 ttccggttgc gaagtgaacc agcgtaccgc gcggtgacga ttttctcgtt gcgaatggcc
    20041 gataaaccag ggttgggtga catagccgta ataacgcgtt ggtacattgc gcgtaaacaa
    20101 ggtcggctgc gtcgaaacgt aaaatccagc acgcgagaca tacatcgcct gttcaaccat
    20161 cgacgaggag ttgtgcgcca gtcgcagcaa gtaaccaact tccagtgcag cggtaatttc
    20221 attgtcgagg ctttcatttt cgcgagacag gaaattcccc tcgctgacta atgcatccga
    20281 tacgccattg actggcaggg tgcgccgtcg gttgagttcg atttgccaag cgtgctcgtc
    20341 gcgatgctgt tcgaactcgg ttacggtatt acgcagagag gtgaaatcca gtggcgccac
    20401 gagagcttcg cgcatgccat tacgcaaaaa gatcagtttg tcgacgttat actgcaaatg
    20461 cttatcgagc gcgttggcta cgttttccag atgattacgc tagctggaga tataggcatc
    20521 ttccagcacc accacttcac gccaggtgag caaggtggaa aaaagcaata ccacgataaa
    20581 gcagagatta acgacatgac cgggccccag gcggcgtgcg agttttttca accagctctg
    20641 gttttccatt tttgtctcgt gctgcaccct gacgactccc aacctgcttt tttatgattc
    20701 tggcataacc cggcaatagc gtaccgcaaa acagcgataa ttttataatg gtggcatgat
    20761 aagacgcgac tatcattcat tgcaggcatt ggaacaccag gccggatgtg atccggcctg
    20821 gtgttaatga taccctttca aacttcttcc gcagaacgac tcccctcccc cggaacaaga
    20881 tcggtttcat gaaaagcttc ccgcttgacc ccgtaaccgt cataccaacg acactcaacc
    20941 ataccgctgg agtatccagt gacaatcatc cgcgggccgc cctctttaac cgtaacttcc
    21001 tcactaacca taaagctcat actcgcctcc ttttttcgag tgaaacttgt tcaccttagt
    21061 tgaagatggc gaattttgca taatagccag tgcgagtatt aatgtgcctg accgcgcatt
    21121 ttcgctactg ctttcactcc cagcaccacg atgccgccga tgataaatcc aagaatcaga
    21181 tttaaaacag tcggtaatat catcgccacc actgcacttt gctgcccggc gatatgttca
    21241 atagcgtgat gcagcggcgc aataccatgt accacaatcc cgccgccgac gaggaacatc
    21301 gccagcgtgc cgacaatcga taatgctttc atcagccaag gcgcgataat caataaccct
    21361 ttacctaatg cctgcatcag cgcgctagat ttttccgcca gccaataccc taggtcatca
    21421 atcttaacga taacccctac cagaccgtag acgcccacgg tcactaccag cgcgatgcct
    21481 gaaagcacca gcacctgatt aagcaacggc gcctctgcca caatccccag cgtgatggcg
    21541 acgatttccg cagacaaaat aaaatcggta cgaatcgccc ctttaatttt gtccttttca
    21601 aacttcagcg gatcctgcgc cgccagcttc tccagacgct gctggctctg cgctggatct
    21661 tctttatgtt tacgcgcctc cagcatatgc agcactttct ctaccccttc aaagcagaga
    21721 aacgcgccac caatcatcaa cagtggcgta atcgcccacg ggataaacgc actgatgatc
    21781 agcgccagcg gcaccagaat cactttattg atcagcgatc ctttcgccac gccccagacc
    21841 acgggaagtt cccggttggc ccgcacgcct gaaacttgtt gcgcattgag cgataaatca
    21901 tcccctaata caccggcggt tttcttcgcc gccagtttgc ccatcacaga gatatcgtcc
    21961 agcagtgtgg cgatatcatc gagcaacgtc agtaaactac tcccggccaa aagcattccc
    22021 tctcattttt attgttgaag ggtgtaagta tgaagcaaaa acggcagctc tgatacttgc
    22081 ttcacaggtc aacatttatt taacaatcaa gtgcaattat atctctcgcc agcagaatga
    22141 ttttcacgtt tactatgaac caccttttta tttccgctgt gagggattat gcgtttccgc
    22201 cagttgttac cgctttttgg cgcgctgttt gcgttgtata tcatttgggg ctcaacctat
    22261 tttgtcattc ggattggcgt gggaagctgg cctccgttaa tgatggcggg cgttcgattc
    22321 caggcagccg gtattttatt gctggcattt ttgctactgc gcggacacaa acttcccccg
    22381 ctacgtccgc tgctcaatgc cgcgctgatt ggcctgttat tgctggctgt cggtaatggc
    22441 atggtgacgg ttgccgaaca tcaaaatgtt ccttccggca tcgccgccgt agtgattgca
    22501 accgtgcccc tctttaccct gtgcttcagt cgcctgtttg gcattaaaac gagcaaactg
    22561 gaatgggtgg gtattgccat tgggcttgcc ggaatcatca tgctcaatag cggtggaaat
    22621 ttaagcggca atccgtgggg cgcgattctg attttaatcg gctcgattag ctgggcgttt
    22681 ggctcagttt atggctcgcg cattacctta cctgtaggga tgatggcggg tgcgattgag
    22741 atgctggcgg caggcgtggt gttaatgatc gcatcgatga ttgcgggtga aaaactgacg
    22801 gcgctccctt ccctgtcagg cttccttgcg gtcggctatc tggcgctgtt tggttcgatt
    22861 atcgccatta acgcttatat gtatttaatc cgtaatgtca gtccggctct cgccaccagc
    22921 tacgcctacg ttaacccggt ggtcgcggtc ttgctgggta cgggactggg tggcgaaacg
    22981 ctgtcgaaga ttgaatggct ggcgctcggc gtaattgttt tcgcggtggt actggtcacg
    23041 ttgggaaaat atctcttccc ggccaaaccc gttgtcgcgc cagttattca ggatgcatca
    23101 agcgagtaaa tgaatcccct gcgtgtcgat ctgcgcgctg gcaccttcgc cgcagatcca
    23161 ctcttccaga cgctcggtaa gcgccgcatc cgtcagcttc tcgcgcctac gtaacgcaca
    23221 ctcccagaca atcagtacgc gccagccgag ctcctgcaag cgactgatat cgcggcgatc
    23281 gcgttcaaca tttttaccta tcttctccag ccagaactca gttcgagtcg caggcacttt
    23341 aaacagatag cagtgatgat gatgccagaa gcagccgtgg gtaaatatca cgcagcgata
    23401 ttcatcaacg acaaaatccg ggcgtccggg cagactggcg tcctgaacgc gaaacgcaag
    23461 gccctgcccg gttaacaaac tggcgaggcg cttctcaatc gccgtatcac gcgtggcaat
    23521 cgcgcgcata tttttgctgc gggtggcctt atcgtgaacg tcgaccatgt tgtgcctctt
    23581 gctgacgcaa cgccaccgcc tgtttgattt ttggctcaag cagttttgcc accgcggcaa
    23641 agaccggcac gaccaccgag ttgccgaact ggcgataggc ctgagtgtcc gaaaccggaa
    23701 tgcggaattt cgcttctccc ggcgcttcaa aacccattaa gcgcgcgcat tcacgaggcg
    23761 ttaaccgacg tggacgatgt tgctgattca gcggatcgtc aaagtctttc tcacccgtgg
    23821 ccatatccca accgcgatcg attaaaattt ccgcgccatc tttgtaataa cgagcagaca
    23881 gcgtacgggt gacgctttgc ggattgttcg gataaaccat tccataaccg aagccgttac
    23941 cgcgcgcctg atgttttttc gcatatcgat agaggtactt ccacagcacc ggcgtcagga
    24001 tatatttcgc ctcaaccatc gggtccagca gctgcgccag cgtcactcgc tgtgcaggga
    24061 aacattcgct gatatcacgc agagtaaaat cggctttcag attcagatcg cgacgaaaac
    24121 ccaccagcac gatgcgttca cggtgctgcg gcaggaaatg tttaccatcg atgattttcg
    24181 gatcgtccgg cccgttatct tctgcatcgg caacgtcata gcccagttcg tccagcgtct
    24241 gcatgatgat gcggaacgtt tttcccttat cgtgactctt caggtttttg acgttttcga
    24301 gcacaaacat cgccggacga cgcgcgtcga taatgcgcac cacatcaaag aacagcgtgc
    24361 cctgggtatc gcaggcaaaa ccgtgcgccc gcccgagcga gttctttttc gatacgccag
    24421 ccagcgaaaa tggctgacaa gggaaaccgg ccagtaaaac atcgtgttca ggaatgtgtt
    24481 gacgaatatg ttccgccgcc gcctcatcac tcacgccttc ttgatgactg agggtgatgt
    24541 cgcggatatc ttcattaaaa tgatgcgtcg ccgggtcgca ataatggttg gctttataag
    24601 tgcgtaccgc atgtttgttc cattcgctgg taaacacgca ctgtccacca atcgattcaa
    24661 aaccacgacg gatgccgcca attccggcaa atagatcgat aaagcgaaac gcataatgcg
    24721 gatgatgttc cggtggtttg ggtaacaacg tttgcagatg ggcaaactct ttctcactta
    24781 aacggtgcca tgccgagtca ttcgccagcg cacgttttaa aattgccgcg ctccagtgat
    24841 tctcacccac accattaagc tgcgccacca gcattttaac atcataaatt tgcagcagtt
    24901 tctccagcat tgcctgtgcg gcattcccgg tgctgtatga atcggttact gatatatttt
    24961 cctgcataga tttaaccggc catctgagat ggccgacagg ttaacataat tactgccgtt
    25021 tagggccacg cgctcagcca gcgggtgaaa acgcatctat caccttgtga tcgatacctt
    25081 cattctcccc cgccagttcg gcaccgagct tcgccatcaa acagcgccaa gctcaatgcc
    25141 cctgaaacgg caaatacgcg cgccaggcca atcgccccca gggcttccag ccgatccgca
    25201 tcctggacga ttttagcttc cgttgttaag ggggcgattt gcgcgctgaa actgtgagcg
    25261 gcaatggcat gacaaacggc ctcaattttc tccgccggaa attgcacgaa ctcttcacgg
    25321 agcaggcgac gcgtctcttc tgccgccagg attgaagaac gctgccgttg cggatgattt
    25381 ttcgccaggc tcacgatatc gtgaaaataa caggcggtta aaatcaccag catatcgaca
    25441 tcgtcatctg ccgccagttt ctgtgccgta gcccagacac ggcgaaaatg acacaggtca
    25501 tgcgccgcgt cctgatgttg atgatggttt ttcaaccagt tctcgaactg tgcctgccag
    25561 tgttgtaact ccatacgcgc tccgttgcca aagagtcgca accttagcag tttttattca
    25621 tagcgtctgc aacgtgcggg acgaggtcgg gtgtaccatg caatgccgcc taacaaggca
    25681 ccaaccaggg ccataaacag aaatccgacc aatgctccta accacttccc cgcggtagat
    25741 cctaaatcac tttcaaatcc cgacacgggg gcatcgggga tccggctcat cacccagatg
    25801 tatcgcgcca ttgcccagac gaaatagcca caccatgtat agaaagccca caatgaatcg
    25861 ccacggataa tctagacact tccgagccgt tgataatact ggttttcata ttctgtcggt
    25921 gacatctgtt cgctagaacc atgccgacgc ttactgttat aaaacatttc gatgtaatca
    25981 aaaatatcac tgcgggcttc ttcccgcgtt ccgtagatct ttttctttat ccgttcacgt
    26041 ttcaacaact ggaaaaaact ttctgcaacc gcattatcat ggcagttacc gcgacggctc
    26101 atgctaccct ccaggccgtg tgatttcagg aacga
//
LOCUS       DAJVPP010000058        25790 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVPP010000058 DAJVPP010000000
VERSION     DAJVPP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.058"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1184
                     /gene="mdtO"
                     /locus_tag="OQM34_002381"
     CDS             <1..1184
                     /gene="mdtO"
                     /locus_tag="OQM34_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1452793.1"
                     /translation="QCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            1181..2647
                     /gene="mdtP"
                     /locus_tag="OQM34_002382"
     CDS             1181..2647
                     /gene="mdtP"
                     /locus_tag="OQM34_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1452794.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            2845..3084
                     /locus_tag="OQM34_002383"
     CDS             2845..3084
                     /locus_tag="OQM34_002383"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452795.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            3094..3264
                     /locus_tag="OQM34_002384"
     CDS             3094..3264
                     /locus_tag="OQM34_002384"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1452796.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            3313..4725
                     /gene="fdhF"
                     /locus_tag="OQM34_002385"
     CDS             3313..4725
                     /gene="fdhF"
                     /locus_tag="OQM34_002385"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS1452797.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            4726..4992
                     /locus_tag="OQM34_002386"
     CDS             4726..4992
                     /locus_tag="OQM34_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCS1452798.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            5086..5775
                     /locus_tag="OQM34_002387"
     CDS             5086..5775
                     /locus_tag="OQM34_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1452799.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(5853..7166)
                     /gene="gltP"
                     /locus_tag="OQM34_002388"
     CDS             complement(5853..7166)
                     /gene="gltP"
                     /locus_tag="OQM34_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1452800.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(7508..8104)
                     /gene="nrfG"
                     /locus_tag="OQM34_002389"
     CDS             complement(7508..8104)
                     /gene="nrfG"
                     /locus_tag="OQM34_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1452801.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(8101..8484)
                     /gene="nrfF"
                     /locus_tag="OQM34_002390"
     CDS             complement(8101..8484)
                     /gene="nrfF"
                     /locus_tag="OQM34_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1452802.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(8477..10195)
                     /locus_tag="OQM34_002391"
     CDS             complement(8477..10195)
                     /locus_tag="OQM34_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1452803.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(10215..11171)
                     /gene="nrfD"
                     /locus_tag="OQM34_002392"
     CDS             complement(10215..11171)
                     /gene="nrfD"
                     /locus_tag="OQM34_002392"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1452804.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(11168..11839)
                     /gene="nrfC"
                     /locus_tag="OQM34_002393"
     CDS             complement(11168..11839)
                     /gene="nrfC"
                     /locus_tag="OQM34_002393"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1452805.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(11836..12402)
                     /gene="nrfB"
                     /locus_tag="OQM34_002394"
     CDS             complement(11836..12402)
                     /gene="nrfB"
                     /locus_tag="OQM34_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1452806.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(12447..13883)
                     /gene="nrfA"
                     /locus_tag="OQM34_002395"
     CDS             complement(12447..13883)
                     /gene="nrfA"
                     /locus_tag="OQM34_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1452807.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            14276..16234
                     /gene="acs"
                     /locus_tag="OQM34_002396"
     CDS             14276..16234
                     /gene="acs"
                     /locus_tag="OQM34_002396"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCS1452808.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            16434..16748
                     /locus_tag="OQM34_002397"
     CDS             16434..16748
                     /locus_tag="OQM34_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCS1452809.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            16745..18394
                     /gene="actP"
                     /locus_tag="OQM34_002398"
     CDS             16745..18394
                     /gene="actP"
                     /locus_tag="OQM34_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS1452810.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            18572..19864
                     /locus_tag="OQM34_002399"
     CDS             18572..19864
                     /locus_tag="OQM34_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1452811.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(20018..21667)
                     /locus_tag="OQM34_002400"
     CDS             complement(20018..21667)
                     /locus_tag="OQM34_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1452812.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(21818..23167)
                     /gene="ghxP"
                     /locus_tag="OQM34_002401"
                     /gene_synonym="yjcD"
     CDS             complement(21818..23167)
                     /gene="ghxP"
                     /locus_tag="OQM34_002401"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1452813.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(23873..24337)
                     /gene="soxR"
                     /locus_tag="OQM34_002402"
     CDS             complement(23873..24337)
                     /gene="soxR"
                     /locus_tag="OQM34_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1452814.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            24423..24746
                     /gene="soxS"
                     /locus_tag="OQM34_002403"
     CDS             24423..24746
                     /gene="soxS"
                     /locus_tag="OQM34_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS1452815.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(24749..25790)
                     /gene="pdeC"
                     /locus_tag="OQM34_002404"
                     /pseudo
     CDS             complement(24749..25790)
                     /gene="pdeC"
                     /locus_tag="OQM34_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
BASE COUNT         6505 a         6944 c         6538 g         5803 t
ORIGIN      
        1 gtcagtgctg gcaaagcgac tggcggatca gtgaaagtga agcggtggcg gcacgggaat
       61 gtaacctgga gaatatctgc cagacgttgt tacaactggg ccagatgaac ccgaatacgc
      121 cgccaacgcc cgccgccaaa ccgccatcaa tggtcgccga tgcttttacc aatccagact
      181 atatacgcta cgcggtaaaa acattgctcg cctgtttgat ctgttacacc ttttacagcg
      241 gcgtggactg ggaaggcatt cacacctgta tgctgacctg cgtgatcgtc gctaatccaa
      301 atgtcggttc gtcgtaccag aagatggtgc tgcgttttgg cggggccttt tgcggcgcga
      361 ttctggcgct gttattcacg ctactggtca tgccctggct ggacaatatt gtcgaattgc
      421 tgtttgtgct ggcaccgatt ttcctgttgg ccgcatggat tgccaccagc tctgaacgct
      481 cttcttatat cggcacacag atggtggtca ccttcgcgct cgccacgctc gaaaacgttt
      541 ttggcccagt gtacgacctg gtggaaattc gcgatcgcgc cctgggtatc atcattggta
      601 ccgtggtgtc cgcgatgatt tacacctttg tctggcctga aagtgaagcg cgcacgctgc
      661 cgcaaaaact ggctggcgcg ctgggtatgt taagtaaagt aatgcggatc ccacgccagc
      721 aggaagtcac ggctctgcgc acttatctgc aaattcgtat cggtctgcat gcggcgttta
      781 atgcctgtga agagatgtgc caacgcgtgg cgctggagcg tcaactggac agcgaagaac
      841 gcgccttact gattgaacgt tcgcaaacgg ttattcgtca gggccgcgat atccttcacg
      901 cctgggatgc cacctggaat tcggcgcagg cgctggataa cgcactacag ccggacagag
      961 caggtcagtt tgccgacgcc ctggagaaat acgctgccgg tctggcaacc gcactcagcc
     1021 gttctcctca aataacgctt gaagagacac ccacctcgca ggccatcctg cccaccttat
     1081 taaaacagga gcaacacgtc tgccagcttt tcgcccgctt gccagactgg acagccccgg
     1141 cattaacgcc cgccacggaa caggcacaag gagccacgca atgatcaatc gtcaactttc
     1201 acgtctgctg ttgtgcagca ttctcggcag cacgacgctg atttccggct gtgccctggt
     1261 acgtaaggat tctgcgcctc atcaacagct caaaccggaa caaatcaaac tggccgatga
     1321 tattcatctt gccagctccg gctggccgca ggcgcagtgg tggaaacaac tcaatgaccc
     1381 gcagttggat gccctgatcc aacggacgct aagtggttca cacaccctcg ccgaagcgaa
     1441 actgcgggaa gaaaaagcgc aatcgcaggc cgatttgtta gatgccggtt cacagttgca
     1501 ggtggcagcg ttagggatgc ttaaccgcca acgtgtctcg gcgaacggct ttttaagccc
     1561 ttatgcgatg gatgcgcccg cactggggat ggacgggccg tactatacgg aagccacagt
     1621 aggtttgttt gccggactgg atcttgattt gtggggtgtg catcgctcag cggttgccgc
     1681 cgccattggc gcgcataatg ccgcgctggc agaaaccgca gcagtagagc tatcgctgac
     1741 cacgggcgta gcgcagcttt attacagtat gcaggccagc tatcagatgc tcgatctgtt
     1801 agaacaaact cgcgatgtga ttgattacgc ggtgaaagcg caccaaagta aagtggcgca
     1861 cggtctggaa gcacaagtgc ctttccacgg cgcgcgggca cagattctgg cggtcgataa
     1921 acaaattgct gccgtcaaag ggcaaatcac cgaaacgaga gaatctctgc gcgcattgat
     1981 tggcgcggga gccagcgata tgccggagat caaacctgtg gcattaccac gagtccagac
     2041 cggcattccg gcgacactct cttatgagtt gctcgccaga cgcccggatc tgcaagccat
     2101 gcgctggtat gttcaggcgt cattagatca ggtggattcc gcgcgggcgt tgttctatcc
     2161 gagctttgat atcaaagcgt ttttcggcct tgatgccatc cacctggata ccttattcaa
     2221 aaaaaccagt cgccagttca acttcatccc gggtctgaaa ttgccgctgt ttgacggtgg
     2281 acggttgaat gccaatctcg aaggcacgcg cgccgccagc aacatgatga ttgaacgtta
     2341 caaccagtca gtactgaacg cggtgcgtga cgttgccgtc aacggcacgc gtctgcaaac
     2401 gctcaacgac gagcgagaaa tgcaggctga acacgtggaa gcaacgcgct tcacccagcg
     2461 cgctgccgag gccgcctatc agcgcggctt aaccagccgc ttacaggcca ctgaagcccg
     2521 gttgccagta cttgccgaag agatgtcatt actgatgctg gacagccgcc gggtgatcca
     2581 aagcattcag ttgatgaaat cgctgggcgg cgggtatcag gcggctcccg tcgtcgagaa
     2641 aaaataaaat gtctgccgcg tgatggctgt cacgcggtat ttcgtttcgt cacgtcaaaa
     2701 ctgacgacag cctgtttttc gtcagagttt tgaataaata gtgcccgtaa tatcagggaa
     2761 tgaccccaaa taaaatgtgg cataaaagat gcatactgta gtcgagagcg cgtatgcgtg
     2821 atttgattaa ctggagcgag accgatgaaa aaagtcgtca cggtttgccc ctattgcgca
     2881 tcaggttgca aaatcaacct ggtcgtcgat aacggcaaaa tcgtccgggc ggaggcagcg
     2941 caggggaaaa ccaaccaggg taccctgtgt ctgaagggtt attatggctg ggatttcatt
     3001 aacgataccc agatcctgac cccgcgcctg aaaaccccca tgatccgtcg ccagcgtggc
     3061 ggcaaactcg aacctgtttc ctgagatgag gcactgaatt acgttgccga gcgcctgagc
     3121 gccatcaaag agaagtacgg tccggatgcc atccagacga ccggctcctc gcgtggtacg
     3181 ggtaacgaaa ccaactatgt aatgcaaaaa tttgcgcgcg ccgttattgg taccaataac
     3241 gttgactgct gcgctcgtgt ctgacacggc ccatcggttg caggtctgca ccaatcggtc
     3301 ggtaatggcg caatgagcaa tgctattaac gaaattgata ataccgattt agtgttcgtt
     3361 ttcgggtaca acccggcgga ttcccaccca atcgtggcga atcacgtaat taacgctaaa
     3421 cgtaacgggg cgaaaattat cgtctgcgat ccgcgcaaaa ttgaaaccgc gcgcattgct
     3481 gacatgcaca ttgcactgaa aaacggctcg aacatcgcgc tgttgaatgc gatgggccat
     3541 gtcattattg aagaaaatct gtacgacaaa gcgttcgtcg cttcccgtac agaaggcttt
     3601 gaagagtatc gtaaaatcgt tgaaggctac acgccggagt cggttgaaga tatcaccggt
     3661 gtcagcgcca gtgagattcg tcaggcggca cggatgtatg cccaggcgaa aagcgccgcc
     3721 atcctgtggg gcatgggtgt aacccagttc taccagggcg tggaaaccgt gcgttctctg
     3781 accagcctcg cgatgctgac cagtaacctc ggtaagccgc atgcgggtgt aaacccggtt
     3841 cgtggtcaga acaacgtgca gggtgcctgc gatatgggcg cgctgccgga tacgtatccg
     3901 ggataccagt acgtgaaaga tccggctaac cgcgaaaaat tcgccaaagc ctggggcgtg
     3961 gaaagcctgc ctgctcatac cggctatcgc atcagcgagc tgccgcaccg cgcagcgcat
     4021 ggcgaagtgc gtgccgcgta cattatgggc gaagatccgc tgcaaactga tgcggagctg
     4081 tcggcagtac gtaaagcctt tgaagatctg gaactggtca tcgttcagga catctttata
     4141 accaaaaccg cgtcggcggc ggatgtcatt ttgccgtcaa cgtcgtgggg cgagcatgaa
     4201 ggcgtgttta ctgcggctga ccgtggcttc cagcgtttct tcaaagcagt tgaaccgaaa
     4261 tgggatctga aaacggactg gcaaatcatc agtgaaatcg ccacccgtat gggttatccg
     4321 atgcactaca acaacaccca ggagatctgg gatgagttgc gtcatctgtg cccggatttc
     4381 tacggtgcga cttacgagaa aatgggcgaa ctgggcttca ttcagtggcc ttgccgcgat
     4441 acttcagatg ccgatcaggg gacttcttat ctgtttaaag agaagtttga taccccgaac
     4501 ggtctggcgc agttcttcac ctgcgactgg gtagcgccaa tcgacaaact caccgacgag
     4561 tacccgatgg tactgtcaac ggtgcgtgaa gttggtcact actcttgccg ttcgatgacc
     4621 ggtaactgtg cggcactggc ggcgctggct gatgaacctg gctacgcaca aatcaatacc
     4681 gaagacgcca aacgtctggg tattgaagat gaggcattgg tttgagtgca ctcgcgtaaa
     4741 ggcaaaatta tcacccgtgc gcaggtcagc gatcgtccga acaaaggggc gatttacatg
     4801 acctaccagt ggtggattgg tgcctgtaac gagctggtta ccgaaagctt aagcccgatt
     4861 acgaaaacgc cggaatacaa atactgcgcc gtgcgcgtcg agccgatctc cgatcagcgc
     4921 gccgccgagc agtacgtgat tgacgagtac aacaagttga aaactcgcct gcgcgaagcg
     4981 gcactggcgt aatacagtcc tttctacagc ctcctttcgg aggctgtttt tttatccatt
     5041 cgaactcttt atactggtta ctccctaccc aatcgtatta tcaaaatgaa aaaaattatc
     5101 gcattgatgt tgtttttgac attctttgcc cacgcgaacg actccgagcc tggcagccag
     5161 tatttaaagg cagcagaggc cggggaccga cgtgcacaat attttcttgc cgacagctgg
     5221 tttagctccg gcgatttgag caaagccgaa tattgggcac agaaagccgc cgacagcggt
     5281 gatgctgatg cctgcgcgct gttggcgcag atcaaaatca ccaatccggt cagtctggac
     5341 tatccacaag caaaagttct tgcagagaaa gcggcgcaag cgggcagtaa agaaggtgaa
     5401 gtaacgctgg cgcatattct ggtaaatact caagcgggta aaccggatta tccaaaggcg
     5461 atttcgctgt tagaaaacgc ctcggaagat ctggagaatg actccgccgt cgatgcccaa
     5521 atgctgcttg gtttgattta cgccaacggc gtgggcatta aggccgacga tgacaaggca
     5581 acctggtatt tcaaacgcag ctctgcaatt tcccgaaccg gttattccga gtactgggcg
     5641 ggaatgatgt tcttaaacgg tgaggaaggt tttatcgaga agaacaaaca aaaggcgctg
     5701 cactggttga acctgagctg tatggaaggg tttgataccg ggtgtgaaga gtttgaaaaa
     5761 ttaaccaacg gttaaggtag gcctgataag acgcggcaag cgtcgcatca ggcatccaca
     5821 cattgccggg ggatatcccc ggcaatcttc aattattgat ccgcagtttt atcaaatttg
     5881 cccagcactt cacgctcata agccagcgcc tttttgcggt caaatttgtg ttcccacttg
     5941 gcaatcacca gcaccgccag cgcattaccc accacgttca gcgcagtacg cgccatgtcg
     6001 aggatacggt caacaccagc aataaacgcc agaccttcca gcggaatacc tacgctaccc
     6061 agcgttgcca gcaacaccac aaacgacacg ccagccacgc cagcaatccc tttcgaggtc
     6121 accatcagcg tcagcaccag aatgatttcc tgccagatgg acaggtcaat gccatacagc
     6181 tgagcgatga aaatagcggc aatactttga tacagcgtcg aaccatcaag gttaaaagag
     6241 taaccggtcg gcaccacgaa gctggtgatc gacgccggtg ctccgtaggc ttccatcttc
     6301 tcaataattc gcggcagtac gctttcagag ctggcagtgg agtacgccag aatcagctca
     6361 tctttcagaa tacgaatcag gatccagacg cttaacccgc acagacgtgc cacaattccc
     6421 agcactacca gcgcgaagaa cagaatggcg aaatgcacca gcagcaccag tttcgccagt
     6481 ggccacagag acgagaaacc aaagttagcc accgtcaccg caatcagcgc aaacacaccc
     6541 accggtgcat aacgcatcac catgtgagtc actttaaaca tggtttcaga gatggagcgg
     6601 aacacggtca ccagcggttc acgatgcgtc gcgggcaggg aagaaagccc cagaccaaac
     6661 agcaccgaga aaaagatgat cggcagcatt tcgcctttcg ccatcgacgc cacaatgttc
     6721 gtcggcacca gcgacaaaat cgtgcccata atgccgtggg aactgctttg taccgcttcc
     6781 gtagtgctct gatatttcga gatatcgacg gtcgccagct gcgacatatc caccccggca
     6841 ccgggctgga agacgttcgc cagagtgatc cccaaaatga tggcgacggt ggtgatcacc
     6901 tcgaagtaaa taatggtttt cgcgccaata cgcccgagct gtttggcatc accaacaccc
     6961 gcgataccca ccaccagcgt ggagatcaca atcggcacaa caatcatttt gatcagatgg
     7021 atgaagatat cacccgccgg agagagcaaa ttgacgacca gccagtcgcg gctgtcgcta
     7081 tggtagtgca ggtagcttcc caggagaatg cccagcacca tagcaaacag aatctgccag
     7141 gccaggctga attttatatt tttcatcaga ataacttcct caatggagcg ccgttccccg
     7201 ttcagaaacg cgagaacgtg acatactgcc agggcatgaa ttgtgttgcg ttggtttgta
     7261 gattttttta acgcttatga tcagtatcat ttgcgcggta tgttgcgcaa ccctttaaca
     7321 acagagcttt tcgctgcatt aatgcataat aacgcgagtt tatggcagga ttaatgaata
     7381 accatttatt atgggaagat ttttatttgt ttaatctgca ataatactga ttggtgatta
     7441 tttttggcat atgttcattc gattattttt tatgcagctc aatgacataa tcttcatcac
     7501 cagatgatta atccgatctc cgctgcaaca gttttgccat attaatcgac tcgaccagct
     7561 gtgtccggtt gatccggggt gagttgagat ccatcacttt ttgccatagt tcgatggctt
     7621 gcgcgtaatt cgcctgcata aacgcatcgg aagccagcag catcagagcg gtgatttcgt
     7681 tagagtccag cgcgagggct ttgtcgatca ttgcgcgagt ctgggcggtc atatgctggc
     7741 tggcctggta ataaagcacc gtcgccagcg ccgcatacag ttcagcgttc tcaccacgta
     7801 gttgcaacgc ctgacgatac gccagcagcg aattgctgta atcgttttgc cacagatagt
     7861 actcgcccag taacgcccat tgttcgctgt tttgtggatt agcgcggatt ttatcctgca
     7921 atgcctgaag ctgcgcttcc ggggtttgct ggctggcaaa ctgatgtagc ggatcgcgct
     7981 gacgctgata ctccgcacgt accgcctgcc attttgggct taacagataa ctgccgacac
     8041 agaggaatac catcaatatc gtgagtgttg tcagcatttt caccggtatt tgaggctgtt
     8101 tcatcgctta gccctcactc gccagaggat cagtgccatc agcaataaca acaccactgg
     8161 cagcgcccac aacaccagcg tctgacccgt taacggtggg ttatagcgaa caaaatctcc
     8221 gtagcgttcg gtcatccagc caatgatttc gacttcgttc ttcccctccg acaccatgct
     8281 ataaacctga tggcgcatac tgaccgccac cggcgcattg gattcgagta agttttgatt
     8341 ctggcactgc ggacaacgta actggctggc aatatttaac gcctgttgct gttgttgcgg
     8401 attggcgaat tgccaggtgt ctacaacctg agcgcgcgca aaacaggtaa ataacagcag
     8461 tagcgtgaga agccctttat tcatcgcgct tcttcccccg ccatccgctt aacaatgcgc
     8521 ccgcaatcat caacaatcct cccccccaga tccagcgcac accgctttgt acatacaaac
     8581 gaaaagcgta acgatccgct ctggtttttt cacccatgac cgcataccag tcatggatgc
     8641 cgttccagcg aattgacggt tccatcattt gctgacggcg tgcttcataa aaacgccgct
     8701 ccggcgttaa ttcaccaatg cgttgctgat ggtcaaacag cgccactatc gctttttcgc
     8761 tggtgtaatt gcctctggct tgcagatcga gtcgctcaaa acggaaggtg tatcctgcca
     8821 gcgtcacctg ctgacccggc tgtaaattca ggctgatctc ctgacgactg acactggaaa
     8881 ccacgatccc cgcggcaaat aacagcacgc cagcatgagc taccagcgcc ggaagctgca
     8941 cgcgtttgcc agagacaaac gtcgccaaca caatcaccac cagcatcaac agaccaaacg
     9001 gtaacgtcgc gcggttaaaa tacggcgcgc caacggagag gcgtccccag cccagcaggc
     9061 cgtaaatcat cggataaagc gttcccacca gcacgatcag tagcactgcg caaaacagca
     9121 acagcgtagc gaggattaac atttcccgcg ataaccccga aaaacgcacc gccgggccac
     9181 cgtcccgcgc tcgccagcca tacagagcca aagacgcaag gctaatcagt gcaaacaggc
     9241 tgaacaacgg cacggcgcgg acgttatcca gtgcgaacgc atgaaccgaa accagaatgc
     9301 cagaacggac aattaaggtg cccagcagcg acagcatcag agtaactatc gccagcaaca
     9361 gcgaccagtg gcggaaaatc ccccgctggc gtgtcaggga caaactgtgc agcagcgcag
     9421 tggcagaaag ccagggtaat aaagaggcgt tttccaccgg atcccagaac caccagccgc
     9481 cccagccgag ttcgcagtag gcccaccagg aaccgaggat gatccccgcc gttaatgcac
     9541 tccagccagg taacgcccag cgccagcaaa ttcgggcgct ggccgcatca aactcgccgc
     9601 gcagtagact cgccagcgcc acgctcgccg ctaccatcaa accgccatag ccaaggtaaa
     9661 gcagcggtgg atgaaagata agaccgggat gttgcagcat cggattgaga tcgcggcctt
     9721 cgattgctgg tggaaatatc cgcacgaagg gatcggacca cagcaccaca aacagtagca
     9781 gtgcggcgag catgagagat aaaacggcta gcgtcagcgg aaatagcgga tcgctttgct
     9841 gccgataatg ccaggcaaac agcgcgctcc aggcggaaag cagcagcacc caaagcagca
     9901 gcgaaccttc atgaccgccc cacaccgccg ccagtttgag ttcccacgac agcaggctgt
     9961 agctatgttg ggcgacataa atgaccgaga aatcgctgat gagaaaacaa tacgtcagta
    10021 cgccaaaggc gagcagcagg agcgcgaact gcgccagaat gccgatgcaa gtgagtcgca
    10081 tcatggcagg ccagcgcaac cgcactcccg caaaggccgt caacggggtc aacacgttga
    10141 ccccgagact taacaacaac gccagaaaac cgacttcagg aaggaatacg tccagaaacc
    10201 acctcttttc tggcttacgc caccgtcaac tgtccggcgt agagaatgaa aaagcgcagc
    10261 atcaacacgc cgaccagact ggccccacac gccgccagca cggcaggaat gccggaactg
    10321 cgattgaccc acggtttgag caacattggc acaatcagcc ccagcccggc gacaccaagc
    10381 cagaaccacc acgtccagaa accgccacct aatgccgcca ccagcgcacg aactttaccg
    10441 tcatcgccca gcgccagacc gacaaaaaac gccaccagca ggaagatttc accccatacc
    10501 acggggattt ccatccggtg aacaaagtgc gcttccgtgg aatgcgggtt actgcgttgg
    10561 cgtatcgcca tggcgatcag cgccaccgcc gcaccggacg agatgccgga gaagaggagc
    10621 agcaccggca ggctcgggtt attgaggaac ggatacgatt tcagcgccga cagcagaaag
    10681 ccggtataag cccccagcag caccgccaac accagcatca gcgtttccag tccgcgatga
    10741 acgggcgtca gcaggctcag aaccttttgc acgatcccca gcttcggcaa ccagcgttgt
    10801 tgcagggcaa gcaaatcatg ttcaaagatt ttcgccagcc acagcaccag caccaccatg
    10861 tagagctgaa acagcatcac ccccatcgac atcaccgagg taaaactgta gtggaacatc
    10921 agcttccaga aggtccacgg tcttgtcagg tggaagacga ggatcaacag accgaggatc
    10981 accgcgcccg gcccgacaat cagcgtggtg cgcagcaacg tactgtctgc accgcccgcc
    11041 tgcgggtaga agcgacgtaa cagcacggcc agcgtcacca gaccggcaga aataccaatc
    11101 aaaaacaggt agatggcaat cggccagtcc cacaccagcg attcaaaatg aaatgcggaa
    11161 gtctgcgtca ttggctcacc tccccgtatt taaacggtac gcggtacagc ttcggtttgg
    11221 ttcccagcgc cagcttgtag cggtaagtag gcttctggcg cagcagttgc gaaatctcac
    11281 tgttaggatc gtccagattg ccaaacgtca gcgccttcgt cgggcaagct tcaacgcacg
    11341 cgggaagctt accggcctgc aaattggtct tacgacagaa atcgcatttg tccgccgtct
    11401 tcgtgaccgg atggataaag cgcacgcggt acggacaggc ggcgatgcag tactgacaac
    11461 cgacgcagag atcaggatta acatcgacaa tcccgctagc ggcatcacga aaagacgcac
    11521 cggtcgggca gacgtcaacg cacggcgcat gatcgcagtg ctggcaagac ttacggaaga
    11581 agcgatattt cacatcagga aattcgccct gcggctcgct acgaataatc gtcaggcgcg
    11641 agacgccttc cggcaccttg ttcacttccc gacaagcatc catacaggcg gtacagccga
    11701 tgcataacga ctcgtcatgc accatgccgt aacgcacccc attgatattc aacgttttcg
    11761 ccaccacgcg ccctgcggtg ccgctgacgg ctgccagcac gccgacgccg gtgagaaact
    11821 ggcgacgaga ccaggtcatg gctgctcctt aagcaacgga acagacgccg ggttaaagtt
    11881 cggattggtg cgctgatcgc tgtggcaatc gacgcaaatc ttaatccgtc ctttgtcact
    11941 taacgtctgc atcgtatctt gctgtggatg cagggaatgg cagctggcgc acgccacttt
    12001 ggttacgtgg acatcgtgcg gccagaacgc tttttgcaac tgttccggca gatgacagga
    12061 catacagacg ctgttctgct ctccgacctt atacatcggc tcgttaaagc gcatcacatc
    12121 tttcacccct tcgcggtgtt gcggtgatgc ttggccgtga cagttggtgc aggtgaccgg
    12181 caatttgtta ttcgggttga taacggaagc atgtttgcca tgcatacctt cggtatctgg
    12241 tttatgacag tccagacagg cggcatccgg gttacgctgc tgggtaactt cgtagcgatc
    12301 accagatgcc tgcaccgcag gggtagcggt aattccgttc aggctccaca acaggcccga
    12361 cgccagcacc ccagcagtta ataacgaacg taatacgctc atattcactc cattgtgttt
    12421 tccgcccctt gcgaggcgga acggggttat tggcttaaca gaccgttttt acgtgcctgc
    12481 tcttcccact gcgggatcac cgttttgatg aagtcctgct tctcggcctt gatttgttcc
    12541 atgttcaggc caatggcctg ctgggctttc tctttggtcg agatatccgg gatctggatt
    12601 tcatgggtga tacctttggt cgccagcagg cgcgccagtt tggtgcgcgc atccgccgct
    12661 ttatccatcg ccgtaccgag catccgtaga ccttcttccg gtgcgtgcat atgaataccg
    12721 tgggaagcga tcgccagatc ccaacgccac tgggcatgac ggatatcgtc ctgaatcggc
    12781 ttcatttcgg cttccgtcgc gcctgcatcc agcgccgctt tcgcttcgaa gtgagcgtga
    12841 accagttgat cttcaacctt gattttcagg tcgttaatcg attgcttacg ttccgcgacc
    12901 actttttgca aggcagcttt atcctgggta tggcagttcg cacaagtctg ggcgaagtta
    12961 tcaaacggat taccaatttt atggtcggtg tagagtttgc cttcggcgtt ctgcactttt
    13021 ggcatatggc agtcgataca ggtcacgttg tttttaccgt gaatgcccgc tgtccaggtt
    13081 tcatattccg ggtgctgcgc tttcagcatt ggcgttttcg acagggagtt agtccagtca
    13141 gagaaggcaa ttttgtcgta atactgctcc atattttcga ctttcatgcc gtcatcccac
    13201 gggaatttaa ccgctttgtt tttgccgtcg aagtaatact ccacatggca ctgaccgcaa
    13261 accatcgatt gctggtcgaa acgtccggct ttctcaaaag gtttaccaat ggcttccatc
    13321 gcgcgagccg catacggacg ggaaagggtt aactccggtt tgcctttggc gaactctggt
    13381 gaggcggtgt tatggcagtc ggcacaacct aagttgttga cgatttccgg gccgccgcgc
    13441 gcccatttac cgtggaagta gccatcttcg ccgtctttct ggatcagacg cgccacatcc
    13501 gggcttttac aactccagca cgccatcggt agcggaccat cttcagcgtt tttcggcgca
    13561 ccggtacgca gggtttcacg cacatcggtc acagcaaagg catgtccacg aggcttgttg
    13621 taatcgcgcg agaagggata ccccgcccac aggatcacca gccgtggatc ttccgccagg
    13681 gcgtcaacac gctctgactg ttccgaggtg gctttccagg agagatattg atcgtgatgc
    13741 tgcggggcaa aggtttcatt cttcgcttcc acagttacag gttttgcggg agctgccgtt
    13801 tgttcagcgt gaacagaagt gaaaaagaaa aaaggaatca ataagctgaa gatacggcgt
    13861 gcgtttattt ttatccttgt cataggggct tcatccgaat tgcgccattg ttgcaatggc
    13921 ggtttttatt gtttttcacg acagtaaccg cacctacact gtcatgacat tgctcacccc
    13981 tatgtgtaac aaataaccac actgtgaatg ttgtctttaa tcaattgtaa gtgcatgtga
    14041 aataccactt tagagttagt cagtatcttc ctctttttca acagcatgca taactgcatg
    14101 ttcctcaaaa aattaatcaa cttttgttgg tgaccttcaa aaattaccct gccgtttatt
    14161 tgcaccattc tacttttgcg tgatctgtcg cccaaatact aaacaaaact gccaataccc
    14221 ctacatttaa cgcttatgcc acatattatt aacatcctac aaggagaaca aaagcatgag
    14281 ccaaattcac aaacacacca ttcctgccaa catcgcagac cgttgcctga taaaccctca
    14341 gcagtacgag gcgatgtatc aacaatctat taacgtacct gataccttct ggggcgaaca
    14401 gggaaaaatt ctcgactgga tcaaaccgta ccagaaggtg aaaaatacct cctttgcccc
    14461 cggtaatgtg tccattaaat ggtacgagga cggcacgctg aatctggcgg caaactgcct
    14521 tgaccgccat ctgcaagaaa acggcgatcg caccgccatc atctgggaag gcgacgacgc
    14581 cagccagagc aaacatatca gctataaaga gctgcaccgc gacgtctgcc gcttcgccaa
    14641 taccctgctc gagctgggca ttaaaaaagg tgatgtggtg gcgatttata tgccgatggt
    14701 gccggaagcc gcggttgcga tgctggcctg cgcccgcatt ggcgcgatgc attcggtgat
    14761 tttcggcggc ttctcgccgg aagccgttgc cgggcgcatt attgattcca actcacgact
    14821 ggtgatcact tccgacgaag gcgtgcgcgc cgggcgtagt attccgctga agaaaaacgt
    14881 tgatgacgca ctaaaaaacc cgaacgtcac cagcgtagag catgtggtgg tactgaagcg
    14941 tactggcgga aacatcgact ggcaggaagg gcgcgacctg tggtggcacg acctggttga
    15001 gcaagccagc gatcagcacc aggcggaaga gatgaacgcc gaagatccgc tgtttattct
    15061 ctatacctcc ggttctaccg gaaaaccaaa aggcgtactg cacactaccg gcggttatct
    15121 ggtgtacgcg gcgctgacct ttaaatatgt ctttgattat catccgggtg atatctactg
    15181 gtgcaccgcc gatgtgggct gggttaccgg acacagttat ttgctgtacg gcccgctggc
    15241 ctgcggcgcg accacgctga tgtttgaagg cgtaccgaac tggccgacgc ctgcccgtat
    15301 ggcgcaggtg gtggacaagc atcaggtcaa tattctctat accgcaccca cggcgatccg
    15361 cgcgctgatg gcggaaggcg ataaagcgat tgaaggcacc gaccgttcgt cgctacgcat
    15421 tctcggttcc gtgggcgagc caattaaccc ggaagcgtgg gagtggtact ggaaaaaaat
    15481 cggcaacgag aaatgtccgg tggtcgatac ctggtggcag accgaaaccg gcggtttcat
    15541 gatcaccccg ctgcctggcg ctaccgagct gaaagccggt tcggcaacac gtccgttctt
    15601 cggcgtgcaa ccggcgctgg tcgataacga aggtaacccg ctggaagggg ctaccgaagg
    15661 tagcctggtg atcaccgact cctggcctgg tcaggcgcgt acgctgtttg gcgatcacga
    15721 acgttttgag cagacctatt tttccacctt caaaaatatg tatttcagcg gcgacggcgc
    15781 gcgtcgcgat gaagatggct attactggat caccgggcgt gtggacgacg tgctgaacgt
    15841 ctccggtcac cgtctgggga cggcagagat agagtcggcg ctggtggcgc atccgaagat
    15901 tgccgaagcc gccgtagtag gtattccgca caatattaaa ggtcaggcga tctacgccta
    15961 tgtcacgctt aatcacgggg aggaaccgtc accagaactg tacgcagaag tccgcaactg
    16021 ggtgcgtaaa gagattggcc cgctggcgac gccagacgtg ctgcactgga ccgactccct
    16081 gcctaaaacc cgctccggca aaattatgcg ccgtattctg cgcaaaattg cggcgggcga
    16141 taccagcaac ctgggcgata cctcgacgct tgccgatcct ggcgtggtcg agaagctgct
    16201 tgaagagaag caggctatcg cgatgccatc gtaacccaca attgccggat gcgaatcggt
    16261 aacggtttgt aggcctgata agacgcgaca gcgtcgcatc aggcattgat tgccggatgc
    16321 ggcgtaaacg ccttatccgg cctacattcg gcaagggtta cccgagcgtt aaccttctcc
    16381 cataagggag cgggaattaa aacaatccct acattacctc tggagaatct gtgatgaatg
    16441 gtacgattta tcagcggata gaagacaatg cgcatttcag ggagttagtc gaaaaacggc
    16501 aacggtttgc caccatcctg tcgattatta tgctggcagt ttatatcggc tttattttac
    16561 tgatcgcctt cgcgcccggc tggctgggca cgccgctgaa tccgaacacc agcgtcacac
    16621 gcggtattcc aattggtgtt ggagtgattg tgatctcctt tgttctcacc ggtatctaca
    16681 tctggcgggc gaacggcgaa ttcgaccgtc ttaataatga agtcctgcat gaggtacaag
    16741 catcatgaaa agagttctga cggcgcttgc cgccacactc cctttcgcag ctaacgccgc
    16801 ggatgctatt agcggggccg tagagcgcca gccaacgaac tggcaggcga ttattatgtt
    16861 cctgattttc gtcgtgttta cgctcggcat tacctactgg gcatcaaaac gcgtacgttc
    16921 tcgtagcgac tactacaccg caggcggcaa tatcactggc ttccagaacg ggctggcgat
    16981 tgccggggac tatatgtccg ccgcctcatt cttggggatc tccgcgctgg tgtttacctc
    17041 cggctatgac gggctgatct actcgttggg cttcctggtg ggctggccaa tcattctgtt
    17101 tctgattgcc gaacgtctgc gtaacctggg acgctacacc tctgccgatg tggcctctta
    17161 tcgtctgaaa caagggccga ttcgtattct ttcggcctgt ggttctctgg tagtggtggc
    17221 gctttacctt atcgctcaga tggtaggcgc aggtaaactg atcgagctgc tgtttggcct
    17281 taactatcac attgcggtgg tgctggtcgg cgtgctgatg atgatgtacg tcctgttcgg
    17341 cggcatgctg gcgaccacct gggtacaaat tatcaaagct gtgctgttgc tgttcggtgc
    17401 cagctttatg gcctttatgg tgatgaaaca cgtcggcttt agcttcaaca atctgttcag
    17461 cgaagcgatg gcggtacacc cgaaaggtgt cgacatcatg aaaccgggcg gactggtgaa
    17521 agatccgatc tccgcgctct ctctggggct gggactgatg tttggtacgg cgggcttgcc
    17581 gcacattctg atgcgcttct ttacagtcag cgatgcccgc gaagcacgta agagcgtgtt
    17641 ctacgccacc ggatttatgg gctacttcta tattctgacc tttattatcg gcttcggcgc
    17701 gatcatgctt gttggtgcga atccggaata taaagacgcg gcgggccatc tgattggtgg
    17761 taacaacatg gcggccgttc acctggcgaa tgcagtgggc ggcaacctat tcctcggttt
    17821 tatttcagcg gttgctttcg ccactatcct cgcggtggtt gcggatctga cgctggcggg
    17881 cgcatcggcg gtttcgcatg acttgtacgc taacgtcttc aaaaaaggcg cgaccgaacg
    17941 tgaagagctg cgggtatcaa aaatcaccgt actgatcctc ggcgtgattg cgattatcct
    18001 cggcgtgctg tttgagaatc agaacatcgc ctttatggta ggccttgcgt ttgccatcgc
    18061 ggcgagctgt aacttcccga tcattctgct ttccatgtac tggtcgaaac tgactacccg
    18121 tggcgcgatg ctgggcggct ggctggggct gattaccgca gtggtgctga tgatcctcgg
    18181 cccgactatt tgggtacaga tcctcggtca cgaaaaagcc atcttcccgt atgaataccc
    18241 ggcgctgttc tctatcagcg tggcattcct cggcatctgg ttcttctcgg caaccgataa
    18301 ctcagcggaa ggggcgcgcg agcgtgaact gttccgcgcg cagtttatcc gctcccagac
    18361 cggctttggc gttgagcaag gccgcgcaca ttaatctctc cccttccccg gtcgcctgac
    18421 cggggaatac gcttcctctc caacatgcat caccttttcc cataatatta aacaaataaa
    18481 ctcattaaaa aatgagcgat ttttgacagt cgtagaaaat gataatgcag agaatatgcc
    18541 ttttctttct tgttaattat aaggatattt tatgcgctat aatggtttaa ataatatgtt
    18601 tttccctctt tgccagatta acgataacca ctctatcaca agttcatcac atacaaagaa
    18661 aacaaaatca gataattaca gcaaacatca taaaaacacg ttaattgaca ataaagccct
    18721 ctctcttttc aaaatagatg atcatgaaaa agtgataggc ttgattcaga aaatgaaaag
    18781 aatttatgat agtttaccat caggaaaaat cacgaaagaa acggacagga aaatacataa
    18841 atattttata gatatagcgt tatatgcaaa taataaatgt gacgatagaa ttacgagaag
    18901 agtttacctt agtaaagaaa aggatgtatc cattaaggtg gtatatttta taaataatgt
    18961 cgccgtccat aataatacta tcgaaattcc acagatagta aatggtggtt acgatttttc
    19021 acaccttagc ctgaagggta tcgtgattaa agatgaagat ttatccaatt cgaattttgc
    19081 aggttgcaga ctacaaaacg ctattttcca ggactgtaat atgtataaaa cgaattttta
    19141 ttacgccata atggaaaaaa tactttttga taattgtatt ctcgatgact caaatttcgc
    19201 tcagataaaa atggccgacg gaactctaaa tgcatgctcc gctatgcatg ttcaattcta
    19261 caatgcagca atgaatagag ccaatattaa aaataccttc cttgactatt caaattttta
    19321 tatggcgtac atggctgagg taaatcttta taaagtaata gcgccatatg ttaatttatt
    19381 taaagccgac cttagtttct ctaaactcga tttaattaac tttgaacatg ctgatctgtc
    19441 tcgcgtcaat ctgaacaaag caatcctcca gaatataaac ttaattgata gcaaactctt
    19501 ttgtacgtgg ctgacaaata cattcctcga aatggttata tgtaccggct ctaatatggc
    19561 taatgttaat tttaataatg ccaatttaag caactgccat ttcaactgtt ctattttaac
    19621 aaaagcctgt atgtttaata cccgtctcta tcgggttaat ttcgatgagg ctagcgtcca
    19681 gggaatgggc atttccattc tccgtgggga ggaaaatatc cccattgata gtgataccct
    19741 ggtaacacta cagaaattct ttgaagaaga ttgtacctct catactggca tgtcacaaac
    19801 tgaggataat attaatgcag tcgctatgaa gattactgca gatattatgc aacacgcaga
    19861 ttgaggttaa gtcgaagaac agccaatgaa ttgactttac aagagagata acttatttgt
    19921 tttagaatca tgaatgttcg cccgagctaa cctgttaaag gtgcaaaaga ttgcaccttt
    19981 ttcagattac ctcgggcctg gttacgtcgc tttcggctta ctgattttcc tcaattagca
    20041 acgcttcaag caaatcgaga tcgtgcagta atttttgcag cgtttcgttg ctgatctccc
    20101 gcgtggcgcg caggtggtac agttcagcac gttcagaacg caatgccgcc agacggaagc
    20161 gacgctcaag gttctcttcc tgcacggaac tttcaacgtc gttacgtcca tcggcgcgac
    20221 gacgcaggtt accaatgacg cgagaactga cctccgtaag caactggtta tcgatattct
    20281 cttcggtatc cgccgccaga cgctcctcca ttttctggat cgccacaatc gccacttctg
    20341 ccgttgccgc tcgcgcaata cgttcttctt tcaattgttg cgaatggtcc gcgacttcaa
    20401 tgtgttgtag cagaataggc aacatcacca cgccgacaaa cagcgagaag agaatgacgc
    20461 cagccgccag gaacaccagc tcatagcgcg ccgggaagac gttaccatcc ggcaagagca
    20521 gcgggattga gagcacaccg gccagagtga tcgccccacg caccccagcg aaagacgcga
    20581 tcaggatttc tcgtgtggtc cacgaaccaa actccatcgg cttcttcttc aggaagcggt
    20641 tgctgaactt tttcatcgtc cacagccagc cgaaacggac cagcatcagc gccgcatata
    20701 tcagaataat atcggtaaac agcatccaga tttcgacgtt agggtcgatt tctgccgcca
    20761 tcagcgacgt ctccagaata cccggcagtt gcagacctaa cagcaggaac accatgccgt
    20821 taaagacaaa ttccagcatc gcccaggtgc tgtttgcgcg caggcgcatt gccagtggcg
    20881 cacggcgcat cacaccggag cgggtgatgg tcatcccggc ggcaaccgca gcgaggatgc
    20941 ccgaaacgcc aatatgttcg gcaatcagat aagaagcgaa tggcagcaag aacagcagaa
    21001 cgatctgcgt cgcgggttca tcaccgcccc agcggctgag gaatcgcagc gagcgaccat
    21061 acagccagct caccacaaaa ccggcaagaa taccgccaat ggctactttc ataaattcga
    21121 ccgtcgcacc gccgacagtg aagatcatcg tccccatcgc cactgccacg gcaaacttca
    21181 acgacaccag gccggaagcg tcattcatca acgcttcgcc ctgcaaaatg cccatgattt
    21241 ttttcgggat gcgcccttcg ccaacaatcc cggagagcgc cacagcatcg gtcggagaaa
    21301 gtaccgccgc cagcgcaaag gcggggatca gcggaatgcc cggcaccacc cagtaaataa
    21361 ggaagccaat gccgaccacg gtgaccacca ccagcgccag cgcgaggccg aaaatctctc
    21421 gaccatgttc gagaaattca cgggtcggcg ttttccagcc atcagcgaac agcaacggcg
    21481 ggataaacaa gactaaaaag agttcaggat caaactccac atgcaaacca aacgtcggcc
    21541 acgccagtag cgcaccgatg gcgatttgca taagcggaag cgggatctga aagggcatga
    21601 cacgagtgac caccccggac agcgagacca cgagggtcat tatcagtatg gtgaagaaga
    21661 tttccatgcg ttccctgttt gcgatgtttt ttatgatctg agaaagacgt tatgcctgaa
    21721 ttctcatttt ttcagaatat cgcaaaagct cacacaatgt gcggagaaaa taaaaacggg
    21781 cagccattgc cacccgtttt cagaatccga tgaaagatta gatagcccag ccacccgcat
    21841 agaaggtcac cagcgccacg gcgataacga ccgtaccgat gttcaacttg cgccattcac
    21901 cggaaaccag acgaccaatc accagagtcg cgaagccgat catgatgcct gttacgatgt
    21961 tacaggtcag cacgatgaat accgccgtaa ccagacccgc catcgcatca acaaaatcag
    22021 caaagtcgat tttcgccacg ttgctcagca tcagcaggcc aacgtacatc agcgccggag
    22081 ccgttgcata ccccggaacg aggtaagaaa gcggagagag gaacagaatc agcaggaaca
    22141 gcacgccaac ggtgatagcc gtcaacccgg ttttaccgcc cgccgccgta cccgccgcag
    22201 actcgatgta taccgctgcc ggagctgcac ccaccaggcc agagaaaacg ctgctcatgg
    22261 agtcagtggt cagtgctttc ccgccgtcga tgatctgccc atctttatcc agcaggttcg
    22321 cctggccggc gacagcgcgg atagttccgg tggcatcaaa tactgccgtc atcaccagcg
    22381 ccagaacgct tggcaggact acaggattca gcgcgcccat aatgtccagg ctgccaatca
    22441 gtgaattgcc gttttcatcg ctcaatgaag gcatggcgaa aacgccggag aaatggacgt
    22501 taggatcgaa gatcaaaccg acaattgaga taccgataat ggtcagcaga atgccaccag
    22561 ggactttcag tttttccaag ccgatgatca ccgccagacc taccagtgac ataatcaccg
    22621 ggaaggtcgc gaaatcaccc agcgcaacgg gcagaccatc aagcgggttt ttaatcacca
    22681 gaccgacacc attagcggca atgagcagca ggaacagacc gataccgatc cccgtgccgt
    22741 gcgccacacc gtgcggcaag ttgcgcaaaa tccagctacg gatacccgtg gcagaaatta
    22801 ccgtaaacag cacacccatc aggaacacgg cacccagcgc gacaggtacg ctaatatgtt
    22861 gccccagcac caggctgaat gcggtaaacg ccgtcaggga gatggcgcaa ccaatcgcca
    22921 acggcagatt agcccacaga cccatcacga tagaaccgag tccggcaacc agacaggttg
    22981 caacgaaaac tgccgcaggc gggaagcctg ctttacccaa catacctgga acgacgatga
    23041 ccgagtagac catcgccaga aacgttgtta acccggcaac cacttcctga cggacagtgc
    23101 ttccacgttg tgaaatttta aaccaggcgt cgagtgaacc gccggtacgc gctgatggcg
    23161 tagacataag aaaacatccc ctgagagttt aattttcgtc aagatgcgtg gcggacaatc
    23221 ccctgccagc gaaacgttat cttcctgctc caggtttgcg gcaatttggt gcgtcgcaaa
    23281 aaagcaaacg tttagctccg cgcatacaaa acgggtggta aaaaaaagca aacgattatc
    23341 cggcgatcat agcggctttt tcaaccgaag tttgctgctt ttttctcttt tattgtcttg
    23401 acgatgaaaa accaggcaat tgatgcgcaa acgttatcgt cgatgtgcaa gattgcagcg
    23461 ttatcgagcc gtataccgtt ctggattgcg ataaaaaatc gtctgagatg acaaaaatat
    23521 tgcggctaaa aagtatactt ttacactggc gaagaaacgt cggtgacata caattaaagc
    23581 atcaacacca accggaacct ccaccacgtg ctcgaatgag gtgtgttgac gtcgggggaa
    23641 accctcctgt gttccagcgg gatagagaga aagacaaaga ccggaaaaca aactaaagcg
    23701 cccttgtggc gctttagttt tgtgtttgcg gataattcca aaagagctcg atcagtcccc
    23761 tcgccccttc ggggagaggg ttagggtgag gggaacaggc agcaccagtg caaatattta
    23821 ccctcacccc ggccccatag ttaacgatga gtcagcttgt ccttgtggcg ctttagtttt
    23881 gttcatcttc cagcaagcgt gcgccggtac cttcttctcc taagcggtcg cccgggttac
    23941 gcaacgggca atcactgcgc gaaaggcagc cacaaccaat acatccgtcc agttcgtcac
    24001 gcagcgccac taaggtatga atgcgccgat ccaactcttc tcgccattgg gacgaaagct
    24061 gtttccactc tttcgcactt aacgtatgcc cttctggcaa cacgccaaac gcttcaccaa
    24121 tggtcgccag cggaatgcca atacgctgag cgattttgat aattgcgaca tatcgcaaca
    24181 catcacgttt atatcgccgc tgattgccgc tgttacggat actagtaatc aaccctttgc
    24241 tttcatagaa atgcagcgcc gataccgcca caccgctgcg tttcgccact tcgccagggg
    24301 ttagcagcgc tttaatgcgg ggtaatttct tttccataaa tcgctttacc tcaagttaac
    24361 ttgaggaatt atactcccca acagatgaat taacgaactg aacactgaaa agaggcagat
    24421 ttatgtccca tcagaaaatt attcaggatc ttatcgcatg gattgacgtg catattgacc
    24481 agccgcttaa cattgatgtc gtcgcaaaaa aatcaggcta ttcaaagtgg tacttgcaac
    24541 ggatgttccg cacggtgacg catcagacgc ttggcgatta cattcgccaa cgccgcctgt
    24601 tactggccgc cgttgagttg cgcaccaccg agcgtccgat ttttgatatc gcaatggacc
    24661 tgggttatgt ctcgcagcag accttctccc gcgttttccg tcggcagttt gatcgcactc
    24721 ccagcgatta tcgccaccgc ctgtagtttt attgcccgcg cgttaactcc cgcgcgggta
    24781 attgctccat ccattgcata aacacctgcg gcggcatcgc cttcgcaaaa aaccatccct
    24841 ggcaatagcg cacgccgcgt ttgcgcagcc agttaacctg ctcctcagtt tcgacgcctt
    24901 cagcgatcgt ttttaacccc aggctgtgcg ccagctcgat gatgtgttcc gcaatcaaat
    24961 gactggtttt gtgggtggtc agcgtttcaa caaacgattt atcgattttc aaaatatcga
    25021 cattcaatga tttaaggtta tgcaagttag agtagccaat accaaaatca tcaattgcca
    25081 cttcgtaacc agcctggcgg aaagccagaa ttaatcggcg tcattttttc gacatcaaga
    25141 aatgcatgct cagtcacttc aaatttaatt tgctgcggac gcaccgcgta ttgctctgtt
    25201 ttctgattga ttcgcgctat caaccgtgac gtatgaaaat cggaggccga caggttaata
    25261 gaaacatagc gatcggcatg tgttgccagg tacgcgccca gatcgcggaa gacattatca
    25321 ataacataat cagttatctg ttctatcatc ccctcctttt ctgccagcgg aataaactct
    25381 gccggattca ttatttgccc ctgctcacca ggccaacgta acaacgcttc agcgccgata
    25441 catttttctg ttttgatatc gattattggc tggtaataaa gacaaagttg atgtttttcg
    25501 agggcgcgtt gcaatttacg tttgggagat aaatagtttt gtcgaatacg tagccagagt
    25561 aatagcagaa caagactccc caaaataccg gcgggcaacg caaaaataag atgattataa
    25621 aaatgggtta taagacgttg atatgaagtc gcaacaatgg ctgcaattgg gcgttttgtc
    25681 gaataaattg tcgcatataa atagccattt ctttgtacgg ttaaatcctt caaatgaatc
    25741 agcggagaaa acgttgctgc cgaggcctct ttgcttaacg agaaaaaggt
//
LOCUS       DAJVPP010000059        25473 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVPP010000059 DAJVPP010000000
VERSION     DAJVPP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.059"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            12..1034
                     /locus_tag="OQM34_002405"
     CDS             12..1034
                     /locus_tag="OQM34_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCS1452816.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1034..2014
                     /locus_tag="OQM34_002406"
     CDS             1034..2014
                     /locus_tag="OQM34_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCS1452817.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2011..2769
                     /locus_tag="OQM34_002407"
     CDS             2011..2769
                     /locus_tag="OQM34_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1452818.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            2779..3588
                     /locus_tag="OQM34_002408"
     CDS             2779..3588
                     /locus_tag="OQM34_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCS1452819.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            3588..4442
                     /locus_tag="OQM34_002409"
     CDS             3588..4442
                     /locus_tag="OQM34_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCS1452820.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            4468..5025
                     /locus_tag="OQM34_002410"
     CDS             4468..5025
                     /locus_tag="OQM34_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCS1452821.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLAQILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            4962..6422
                     /locus_tag="OQM34_002411"
     CDS             4962..6422
                     /locus_tag="OQM34_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1452822.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(6472..6726)
                     /locus_tag="OQM34_002412"
     CDS             complement(6472..6726)
                     /locus_tag="OQM34_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1452823.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6927..7661
                     /locus_tag="OQM34_002413"
     CDS             6927..7661
                     /locus_tag="OQM34_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS1452824.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKTAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHIIITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7663..8274)
                     /gene="emtA"
                     /locus_tag="OQM34_002414"
     CDS             complement(7663..8274)
                     /gene="emtA"
                     /locus_tag="OQM34_002414"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1452825.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8374..9288
                     /gene="ldcA"
                     /locus_tag="OQM34_002415"
     CDS             8374..9288
                     /gene="ldcA"
                     /locus_tag="OQM34_002415"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1452826.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            9383..11119
                     /locus_tag="OQM34_002416"
     CDS             9383..11119
                     /locus_tag="OQM34_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1452827.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            11730..13144
                     /locus_tag="OQM34_002417"
                     /pseudo
     CDS             11730..13144
                     /locus_tag="OQM34_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13312..14382)
                     /gene="dadX"
                     /locus_tag="OQM34_002418"
     CDS             complement(13312..14382)
                     /gene="dadX"
                     /locus_tag="OQM34_002418"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCS1452828.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14392..15690)
                     /gene="dadA"
                     /locus_tag="OQM34_002419"
     CDS             complement(14392..15690)
                     /gene="dadA"
                     /locus_tag="OQM34_002419"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1452829.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16020..17552
                     /locus_tag="OQM34_002420"
     CDS             16020..17552
                     /locus_tag="OQM34_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS1452830.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(17604..18323)
                     /gene="fadR"
                     /locus_tag="OQM34_002421"
     CDS             complement(17604..18323)
                     /gene="fadR"
                     /locus_tag="OQM34_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1452831.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18544..20085
                     /gene="nhaB"
                     /locus_tag="OQM34_002422"
     CDS             18544..20085
                     /gene="nhaB"
                     /locus_tag="OQM34_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1452832.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20231..20761
                     /gene="dsbB"
                     /locus_tag="OQM34_002423"
     CDS             20231..20761
                     /gene="dsbB"
                     /locus_tag="OQM34_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1452833.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(20807..22075)
                     /gene="umuC"
                     /locus_tag="OQM34_002424"
     CDS             complement(20807..22075)
                     /gene="umuC"
                     /locus_tag="OQM34_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS1452834.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22075..22494)
                     /locus_tag="OQM34_002425"
     CDS             complement(22075..22494)
                     /locus_tag="OQM34_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1452835.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            22867..23767
                     /gene="hlyE"
                     /locus_tag="OQM34_002426"
                     /pseudo
     CDS             22867..23767
                     /gene="hlyE"
                     /locus_tag="OQM34_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(23974..24420)
                     /locus_tag="OQM34_002427"
     CDS             complement(23974..24420)
                     /locus_tag="OQM34_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1452836.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(24512..25171)
                     /locus_tag="OQM34_002428"
     CDS             complement(24512..25171)
                     /locus_tag="OQM34_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1452837.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(25243..>25473)
                     /locus_tag="OQM34_002429"
     CDS             complement(25243..>25473)
                     /locus_tag="OQM34_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1452838.1"
                     /translation="DDFSRVPEELMKGFGQPQLAMILPLDGRKKLVNADIEKVKLALT
                     EQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
BASE COUNT         6223 a         6625 c         6286 g         6339 t
ORIGIN      
        1 ggatgttcac catgcccata accagacgga cgtttgcgca ggcacttgca tctaccctgc
       61 tcctgcagtc tctaccgtcc ttttcacaaa cagttaatcg ttttgcttcg caatctcttc
      121 ccgaagccca aaacattaca cgcatcgtca gcgcaggcgc gcccgccgat ttgctcttgc
      181 tggctgtcgc gccagaaaaa atggtcggtt tttcctcttt tgattttgcc cgtcaggcat
      241 taattccatt gccagagcac attcgccagt tacccaggct gggacgactc gccgggcgcg
      301 ccagcacact atcgctggaa gggcttatgg cgttacatcc cgatttggtc gttgattgcg
      361 gcaatacgga tgaaaccttg atctcccagg cacggcaggt tagcgaacag acacaaatcc
      421 cctggttatt gcttaacgga aaactggcgc aatcagcaga acagttaaca acgcttggca
      481 aaacgttagg cgaagagcac cgcgccgcag aacaagccaa tctcgccagc cacttcgttg
      541 gtgaagctca ggcattcgcc acctcacccg ccgctaacct ccgcttttat gctgcgcgcg
      601 gtccccgagg gctggaaacg ggcttacagg gttcgttgca taccgaggcg gcggaattat
      661 taggtttgca caacgtcgcg caaatagccg atcgccacgg cctgacacag gtttccatgg
      721 aaaatctcct gcgctggcag ccggatatta ttctggttca ggaggccgtt actgcagatt
      781 ttattcgtcg tgatcctctc tggcagggcg tgaaagccgt tgcggaacaa cgcatcttat
      841 ttttaagtgg cttgcccttt ggctggctcg atgccccgcc gggaatcaac cgtcttctgg
      901 gattacgcag acttcacgcc tggctggatc ccgccatcaa tcgccagttt aaaagtgaca
      961 tgcagcatta cgcccaactg ttctggcatt gttcactcag tgatgccgac tatcaaaaat
     1021 tggtggcgag ctaatgagaa tcgtaaacgg atgtattttg ctgctggcag ctatcagtat
     1081 cacttttgcc gccgtaagtg gggcttatca tctcgatatg cagcaactgc tggcgctaat
     1141 tcttcgtcaa gaaaatgagt ctgtgcagga acaaattgtc ttctggcaaa tccgcgtgcc
     1201 gcgtattctt gctgcgctgt tactgggcgc agcgctggca ggagccggaa ccacctatca
     1261 gggaatgttg cgtaatccct tagtttcacc ggatatttta ggcgtctccg ccggtgccgg
     1321 acttggtgcc tgcgcggcaa tcctgtgggg attacccatt gtttacatcc agctgtacgc
     1381 attttgcggc gggctgatgg tggtcgcggg cgtctggttg attacgcgtc gggtgacccg
     1441 ccacgatccg atcttaaccc tggtgctggt aggcattgcg ttaggaacgt tgtgcggagc
     1501 ggggatatcg ctaattaaaa cgctggccga tccttacacc cagttaccgt caattacctt
     1561 ctggttgctg gggggactgt ccaccgtgac cttacgcgat ctctgctatg ctgcaccgat
     1621 cattctcata ggctccttgc ccctgttttt tctccgctgg cgcatgaatt tactcacgct
     1681 ttccgacgac gaagcccgtt cgctgggact gaacgtgacg cgtctgcgct ttggattgat
     1741 tgtctgcgcc acactaatca ccgccagcac ggtagcaata gcgggcataa ttggttggat
     1801 cggtctggtc gttccgcaca ttgcccgctt gttgaccggt cataaccatc agcagctgtt
     1861 gcctatggca atgtgtactg gtgctattct gctcctgctt accgatacgc tggcgcgcag
     1921 cattggaaca acagaaatcc cacttggcat tctgaccgct tttgttggcg caccgttttt
     1981 cctgcttttg ttgttacgcg gaggtcgcca gtgactttgc ttgccgttcg tcatgcgtct
     2041 ctgggttact cccgccatcc agtgctacgc gacgtgtcct tcaccctgtc gcaaggaaca
     2101 atttgttgtc tgcttggcgc gaatggttgc ggcaaaacca cactcatgcg ctccattctt
     2161 ggcgttatac cgttattaaa aggcgagatc ctactggata gcctctctgt gcagacactc
     2221 agccatcgcc agcgcgcaca ggctattgcc tgggtgccgc aggcgcacga cggtattttt
     2281 gctttcagtg tgctggatat ggtgttgatg ggcctggccc ccaccatagg cgctttttcc
     2341 gttcccggta agcaagaacg cctgaaagcc acggagcaac tggaaaagct gggcattttg
     2401 catctggcga cacgacgctg gaatacgctt agcggcggtg aacgtcagct ggctttaatc
     2461 gcccgtgcac tcgtccaaca accgcgcttg ttgttactgg atgagcccgc ctccagcctc
     2521 gatttcggtc atcagatcca acttctggac acgctggcgc agctcaaaaa taacggcatg
     2581 acaatgttaa tgtcgactca ccatccgctc catgccaacg ccattgccga cagcattatc
     2641 caagtagagc cagatggacg agtaacacaa ggcttaccga ccgaacagtt aacaacgaac
     2701 aaactggcgg ccctgtatcg ggtttctgcc gaccaaattc atcatcacct ctctgcaatt
     2761 agccgctaag gaacaatcat gctcattgat gatattgatt ttgcagacct ttatctgcag
     2821 caactcaaac tggctcaccg cacagaaaaa acgcccgatc actgggatca acgggcggag
     2881 aaaatggcgg aaaattgcgc ctcgccaacg gacagctacc tgcaacaact gatcgctaaa
     2941 attgacctgc aaggcgctca gaccttattc gatatgggct gcggaccagg caccgtatcg
     3001 ctggcgctgg ctgacaaact cactacggtt tatggcgttg attacagtca ggggatgctg
     3061 aacgttgccg cccgccgcgc cgccgcactg aaggctgaca atgtccactg gatccagcgc
     3121 gcctgggaag aggactggag tgacctgcca cgctgcgata ttgccgtggc ttcccgctcc
     3181 actctggtgg ctgatatgcg ccatgcaatg agcaaactga ataatcaggc acggcttcgc
     3241 gtctatacaa ctcatctggt cagcacctcg ttcgtctctc cagccattca gcgggcggct
     3301 ggtcgggaag tgatcgaatt acccaactat atctttgcgc tcaacgtcct gtatcagatg
     3361 ggaatttatg cccacgttga ttttatccgt ggacaaaatt gccaacagga taacagcacc
     3421 tgggaacgtt ttgagcagaa cgtcagctgg agtcttggcg cgcttaacga cgatgagcgt
     3481 gagcggttat atcactggta tcaacaacaa gatgctcgtg ccctcgcccc tgccagccgc
     3541 gactgggcgc tcatctggtg ggatagcgta ccgcaggagg cactgtgatg attttccttt
     3601 ctcaggcaca aatcgatgcg ttactgctgg aagatatcca gggaggtgac ctgaccacgc
     3661 gagcattaaa tattggacac cagcctggct atatagcgtt ttttcaccgt cagggcggct
     3721 gcgtcagcgg cgtttctgtc gcgtgtaaga tgttaactac gctgggatta actattgatg
     3781 acgcggtcag cgacggttca caagcgaacg cgggtcaacg gctaatccgt gcgcagggta
     3841 atgccgcagc acttcatcag ggatggaagg cagtccagaa tgtgctggag tggagttgcg
     3901 gtgtttctga ttatctcgct caaatgctgg cattgcttcg tgaacgttat cctgacggca
     3961 atatcgcctg cacccgaaaa gcaattccgg gcacccgctt actggcttcg caggcaattc
     4021 tggctgccgg aggactaatt catcgtgccg gatgtgcgga aaccatatta ttgtttgcca
     4081 accaccgcca ttttcttcat gacaatcagg gctggtcagg cgcaatcgct cagttacgtc
     4141 gccacgcacc cgagaagaaa attgttgttg aagccgacac gccgaaagag gctatcgccg
     4201 cgttacgcgc acaaccagac gtacttcagc tcgacaaatt tagtccgcaa caggcaactg
     4261 aaattgcgca aatagcgccg tcgctggctc ctcactgcac gctggctctg accggcggaa
     4321 ttaatctgac gacactcaaa aattacctcg actgcggcat tcgccttttt attacctccg
     4381 cgccttatta cgcggcacca gctgacatta aagtcagtct gcaacccaca gcctctattt
     4441 aattctttgt taaacggaat ttattctatg agactcaaaa aacgttatct ctgcacagca
     4501 ttatcacttg cctttaccca gcaggccgta gcggctcagg agagcgacac gctgaccgta
     4561 tggtccagtc cggtatcatc gacgacgacc accgttctcg atcaacccac catgaaggcc
     4621 ttggataaac agaatgtcgc tcaggcatta agtgtcgtcc ccggcgtggt gctgcaaaag
     4681 tcaggcagcc gcaacgaaga acaggtaaaa gttcgtggct ttgatagtcg gcaggtgccg
     4741 gtctatttcg acggtgtgcc catttatgtc ccctatgacg gcaacctcga tctggcgcag
     4801 attctgacca acaatctggg ggcagtcgaa gtttccaaag ggtattcgtc gctgcttcat
     4861 gggcctaatc agatgggcgg agccattaat atcaccaccc tgaagaagca agtctgggat
     4921 atcgccaggg atggagccgt agtcaggaca atgcctacga tatgcatgct tcatttgccg
     4981 ccagcagcga tcttgggtat ttgcaagtca gcggtagcca gctaaagcag gattttctcg
     5041 gcctgccgca tggtgtaaat aatgatattg caggcaaaca cggcaagatg attaattcat
     5101 cggctgatga taaacgcggc attgtgaagc tcggttttac accgcgcgaa aacgatgaat
     5161 acacgctgac ttacattaag caggatggtg aaaaagataa cccgccatac agcggaaatt
     5221 gtggtcaaaa atcacgctac tggcagtggc cagagtatga caaagaaagt ttttattatc
     5281 agggagcgac ccaactaaac gaccgtttta ccctgaaaag tcggctgtat cgcgacacct
     5341 ttgaaaatac gctgatgatg tacaactcgc tggctgattt gaaaaataaa aaaggcagct
     5401 acagccatta ttccgattac agcgacggtg ccggattaca actggcagcc gatgtgcgtg
     5461 aaaacgatct gctgtcgttt gccgttaact ggaaagatga cgtacaccgg gaaaaaggtg
     5521 cgccgcacgc cgcttacgat cgctatgaag atcgtacctg gtcgctcgcc agtgaatatc
     5581 aatgggctgc tgctgataat gtcgatgtcg tggcgggaat cagctatgac tggcgcgata
     5641 gcgtagaagc gaaaaaacac gagaaagatg gcagcatcac ccattatgac gacaacaatc
     5701 agtcagcttt taactggcag gtgatgggga aataccactt tgccaatgaa gacactctgg
     5761 cgctttcgta ctatgaccgc acacgctttc cgacgctgaa agaacgctat accacatcca
     5821 aacctgcgta taaccagata gcgattgtta acccgcagct caagccggaa cgcgctcgcg
     5881 gggtggattt aacctggaat ggtgccttca cgcgcgactg gggatttgag gtcagcgttt
     5941 actataaccg ggtgagtgat gccatcctct cgcacaatat caatgccgat accattcaaa
     6001 atcagaacag cggcacggtg gattacagcg gtctggatgc cggtattaag gggaaaatca
     6061 gcaatatact ggatgtagga ttgagctacg ctctgatcca cgctgacgcc aaacgcaaag
     6121 acatcggcaa gataaccgat cttccaacgc agacaatgac cgcatggatg acactcaaac
     6181 catgggagcc gttaagcgta acgctgtcgg aagaggcgcg ttcctccagc tacagcaaca
     6241 gtgacggttc acaaaaagcc gccggttttg cggtgaccca cattcgagcc gattacacct
     6301 taggtcatgg cttcagcgtc aatgcgtcgg tcaataacct gtttgatacc caatatgcct
     6361 acagtgaagg gtttattgaa gaaggtcgca atttctgggc aggtgtcgag tatacgttct
     6421 gatgtgaaag cggatagcgg catctgtatc gctatccgcc ttatgaaaat cttattctct
     6481 tcgcaggagg cggaatacgc ccagcaccag gatagcgccc accaccgcca ccaggaagct
     6541 gtgcaaatta aaaccgctga tggagccgtc aatgccaaac atggtcgcca gccagccgcc
     6601 gaccaccgca ccgactatcc cgagaataca ggtcaggaaa aatccaccac catcacgccc
     6661 cggcatgatt agcttgccga taatgccggc tatcaggcca aaaataatcc aggcaataat
     6721 tcccatgttc aggccctcgt ttcgatgaat cggtcgcaag aaatgcttac gtcaacgctc
     6781 taagtatagg caagggcggg atcgatgctg ggcgtttgtc ggctgattta agaacaacat
     6841 gtaaaaaata ttcacgcatt tgtattaagt tttgttaact atgaccgata aaccaaagac
     6901 agtttgtcag tcaggagttt ttccgcgtga gtcattacca tgagcagttc ctgaaacaaa
     6961 atccgttagc cgtcctgggc gtgttacgcg atttgcacaa aaccgcaatt cctttgcgtc
     7021 tcagttggaa tggcgggcag ctgatcagca aaatactggc aataaccccg gataaactgg
     7081 tgctggattt cggcagtcaa gccgaagaca acatcgccgt gctaaaggca cagcacatta
     7141 tcattaccgc cgaaactcag ggtgcgaaag tcgagtttac tgttgaacaa ctacagcaga
     7201 gtgaatactt gcagcttccg gcatttatta ccgtaccgcc tcccacctta tggtttgtac
     7261 aacgacgccg atatttccgc atctccgccc cactccatcc gccttatttt tgccagacca
     7321 aactggcgga taacagtacg ttacgtttcc gcttgtatga tttgtcgtta ggcggcatgg
     7381 gcgcattact ggaaacagca aagcctgccg aattacaaga aggtatgcgc ttcgctcaaa
     7441 ttgaagtcaa catggggcaa tggggtgttt ttcactttga cgcccagtta atctccacca
     7501 gcgagcgcaa agtgattgat ggcaagaatg aaaccatcac cactccccgt ctgagcttcc
     7561 gttttcttaa cgtcagcccg acggtggagc ggcaattaca gcggattatt ttctctctcg
     7621 agcgagaagc ccgggaaaaa gcggacaaag tgcgcgactg atttacatcg cgtccagtgc
     7681 ctgctcaagt ttgtagatat agcgcggagc ctgcggcgca ggatgatttc gcgctacgtg
     7741 atcgaggaac tcgtcagcat ccaaatcgtt gattttgctg atcgcctttt tccgatctga
     7801 cgagaaagtc cgtagcagcg cacctgcccc gttagcgtat gacaccacca gcgcatattg
     7861 cagtaccttc ggatcttcaa tgcctgccag cgggccggtt tccagaatat tcaggtaagc
     7921 cgcccccatt gaaatattac gctctggatt tttcagctcg ctggtcgtcg gctcaccact
     7981 ccagcccata cggcgataaa catcacgtcc ggaggttgaa gcttttaact gcatcaaacc
     8041 aatggcattc gatttactca ccgcgttcgg attaccaccc gattcgatag cgataatcgc
     8101 cgtgatcaat tgtggatcga cgccccaggc tgcaccggct ttctggctta ttggcatcca
     8161 ctgcatcgca cgttgcaccg gaactttcgc gttccacggc gggttcgtat agtcatgctt
     8221 tgatgaacaa cccgctaata acacaatcaa aaaggcaaac catctcaatt tcacttcttc
     8281 tatccttata gcctggaatc gccgttgagg tgacaacggc acacctaagc ggcatgatat
     8341 acatttcgtt tctgttgtca gcaaggaatt gccatgtctc tgtttcactt aattgcccca
     8401 tcgggttaca gcattaaaca gcacgccgcg ctgcgtggta tccagcgcct taccgacgcg
     8461 gggcatcaag tcaataacgt cgaggttatt gcccgtcgtt gtgagcgttt tgcgggtacg
     8521 gaaacggagc gtctggaaga tctcaattcc cttgccagac tgactacccc caacaccatc
     8581 gtgctggctg tacgcggcgg ttacggtgcc agtcgtttac tggcagatat tgactggcag
     8641 gcactggtgg ctcgccaaca acatgacccg ttactcattt gcggacatag cgattttacc
     8701 gccattcagt gcggtcttct ggcgcaaggc aatgtcatca cctttagcgg ccccatgctg
     8761 gtagcaaact ttggtgcgga tgaactgaac gcctttaccg agcaccattt ctggctggcg
     8821 ttacgcaatg aaaccttcac cattgaatgg caaggggaag gcccgacatg tcagaccgaa
     8881 ggcacgttgt ggggaggcaa tcttgcgatg ctgatttcac tgattggtac accgtggatg
     8941 ccaaaaattg agaacggtat tctggtgctg gaagatatta acgtgcaccc tttccgcgtc
     9001 gaacgtatgc tgttgcagct ctatcatgcg ggaattttgc cacgtcagaa ggcgattatt
     9061 ctcggtagct ttagcggcag cacgcccaat gattatgacg caggttacaa cctggagtca
     9121 gtgtacgcgt ttttgcgttc ccgcctgtcg attccactca ttaccggcct cgattttggt
     9181 catgaacaac gcacggttac gctgccgttg ggcgcacatg ccattctgaa taacacccgg
     9241 gaaggcgctc agttgacgat tagcggtcat cctgttctta aaatgtaaaa aaatcacgct
     9301 tcagggctaa gtaaaacgct gtctctgccg ctaatatggt agggtttata taagagacag
     9361 cgtaatcagg agtaaccgac cgttggatgc cacaacaata attagccttt tcatcttagg
     9421 atccatcctt gtcaccagca gtatattact tagttcattt tcttcccgtc ttggcattcc
     9481 tattctggtt atttttctgg cgatcggcat gctggcggga gtcgatggcg tcggcggtat
     9541 cccgtttgat aattacccct tcgcctacat ggtaagtaac ctggcgctgg cgattatttt
     9601 gctcgacggc gggatgcgca ctcaggccag ttcctttcgt gtggcgttag gaccggcgct
     9661 gtcgctggcg acgctgggcg tacttatcac ctctggttta accggcatga tggcggcgtg
     9721 gctgtttaat cttgatttga ttgaaggctt attaatcggc gctatcgtcg gctccaccga
     9781 tgctgcagcg gtcttttctc tgctgggtgg taaggggctt aacgaacgtg ttggctcgac
     9841 gctggaaatt gaatccggca gtaatgatcc aatggcggtc tttctgacga ttaccctgat
     9901 tgcaatgatc cagcagcatg aaagcagtgt cagttggatg ttcgtggtcg atattctgca
     9961 gcaatttggc ctcgggattg tcatcggtct tggcggtggt tatttactgc tgcaaatgat
    10021 taatcgcatc gccctgcctg ccggattata tccattgctg gcattaagcg gcggtatttt
    10081 aatttttgct ttaaccacgg cgctggaagg cagcggtatt ctggctgttt atctgtgcgg
    10141 ttttctgctg ggtaatcggc cgattcgcaa ccgctacggc atcctgcaaa atttcgacgg
    10201 cctcgcctgg ctggcgcaaa tcgccatgtt cctggtgctg gggctattgg ttaacccaag
    10261 cgatctgctg cctattgcca ttccggcgct cattttgtcg gcatggatga tattcttcgc
    10321 ccgtcccctt tcggtatttg ctggattgct ccctttccgc ggtttcaatc tgcgtgagcg
    10381 cgtgtttatc agctgggtgg gattacgcgg cgcggtgcca atcatcctag ccgtgttccc
    10441 gatgatggcg gggctggaga atgcgcgact gttctttaat gtcgccttct ttgtggttct
    10501 ggtttcactg ctcttgcagg gaacatcact ctcgtgggcg gcgaaaaaag ccaaagtggt
    10561 ggttccgccc gtcggacgtc cggtgtcacg cgttggcctg gatattcatc cggaaaatcc
    10621 gtgggagcag tttgtttatc aattgagtgc tgataaatgg tgtgtaggtg cggcactgcg
    10681 cgatttgcat atgccaaaag agacgcgtat tgcagccctg tttcgtgata accagttgct
    10741 tcatcctacc ggcagcaccc gactgcgcga aggcgatgta ttgtgtgtaa ttggtcggga
    10801 acgcgatctc ccggcgctcg gtaaactgtt cagccagtcg ccaccggtcg cgctggatca
    10861 acgcttcttt ggtgacttca ttctcgaagc cagcgccaaa tatgctgatg ttgcgctgat
    10921 atatggcctg gaagacgggc gagaatatcg tgataaacag caaacgctgg gtgaaattgt
    10981 ccagcagttg ctaggcgcgg caccggttgt cggtgaccag gtagagtttg ccgggatgat
    11041 ctggacggtg gccgagaaag aagataatga agtgttgaag attggcgttc gggtggctga
    11101 agaggaagct gaatcttaaa tttaatgcag catctatcgc cggatgcgat gctttactga
    11161 acgcagcctg agcctgtaat aaacttgtca tttcctgatt ttgatatgct catcccaaca
    11221 tcagaagaag gttaaattat ggatgacaaa ccattgcagg ctctggctaa tgaactcaaa
    11281 acaccggctc aagaaagtca gcgtaagggc tttttccgac tgtaacaggt aataaaaacc
    11341 agccccgcat taccggtcaa ccatcagggg cccctgcctg acatcgcctg aacatattcc
    11401 cccacgcaac ctgccgcact gacatagccc acacaatagg cgtttcatcc gcctgtagca
    11461 ccggatgcat gagcgatttc ttatagttat tcctttgctt aataacgatt atttccgggt
    11521 cgttcactct cgtattttct ttaatacgtc agagctacct aggccgtaat acagcatgta
    11581 ccagacccga cacggcattt ctgaccaggg aaaaagtcaa cgatgagtag tgatatcagc
    11641 gcaaatgacg tcctgtggct cctttgcggt gaccacattc acgtctccgg cgctgccaga
    11701 ggatatttat ttcaaggaaa cgtagcatta tggctagctt taaagacatc tgcccattcg
    11761 cccccgccat tcccccatct tccgttaacc caggcgtacg acgcccgcca gggcggctct
    11821 tctcaagaat caaattttgc agaacatgaa gtatctcagc ccgcaaaaat tcagctgggg
    11881 tgatgccccc tggcagatca ttgacctcag tatcgcgggc aaagtgaaca tacaggtgga
    11941 caacaatacc atcatcacgt tggggactcg tctgaatcag caacataacg agttcatgat
    12001 ggtcgccaaa tggtgcgaat gggctataca gcaggatggt ttgcaagaaa acctgcaaaa
    12061 aaacctgtac gaaatcctgg aagaaaacca gcaaaacaaa cagtcagaaa ttccccaaga
    12121 agacctgaaa gaaagtctgg aagaaatcaa ggaaaatatc ctggaagaaa acctgccagc
    12181 aagccggatt gaaaaccggg cagaagcatt gcgcagaatg aaggaatgtc tcattacaag
    12241 acggagtatg ctcaatctta gcaacctggg attgacttca ctccctgaga atttgcctcc
    12301 acatctgatt gaattttact gcagtaaaaa cgtgttgacc gcgttaccga aggtgatgcc
    12361 aaagtggctg ctggtgcttg actgtacaga taacgtcttg atcttgttac cgaaggtgca
    12421 gccctcaaaa ctgatggtac tgaagtgcta tgaaaacagt attatctggc tgcctgagct
    12481 gtcgactaac ctgagagtga ttaactgttc tgaaaacttc ttgcaatttt taccgccgtc
    12541 gatgccccag tacctgtata aactatcctg tgccggcaac aatattaact caatacctga
    12601 tgagatgctg gagaacttga ctcgcctgaa agtatttgac tgttctagta acgatttgat
    12661 ctcttcacca cggctgccgc ccaaactaat aatttactac tgtggagaaa acaagtttaa
    12721 aacggtacag gtgccgcagc cccagagcct gaaggtgttt gactgtaatg gtaacccgtg
    12781 ggacaaagac aatttaccga cgctgctcaa agccgtcgag ggcctgaaaa aacagcaggg
    12841 cctgaaggac cttttggact ttttgcacaa ggaaggctag gttgacctgg aaggactcga
    12901 ggagctggag gaccttatgg atctggagtt cctggatgac ccggaactcc tggagcgcgt
    12961 gaaggtacag gaggacctgg agctcctgga tcaacagttg ggcctgttga gtctggaaag
    13021 acaacaggac tcgcagcctg ttaatcaaca atctgaacat gaacccgaat ctgcatcaag
    13081 ggtgaagcgt gatttatctg aagtcgactc cgagtcaaca atgaagcgta agcgttttat
    13141 gtagaacgcc tgtgcggcga gttcaggttc cggggaaata gtgtccattc ttttaatgga
    13201 cactatcatg ggcaatggct ctgtcacatt aacaactaca gttgctgacc agccggatgg
    13261 atgatttttt cgcaaccaga agacgctgcc tccgatccgg cttacaacaa gttacactgt
    13321 cacaaccggg acgcgtaacg ccagcgcgca catcaactca tagcccaccg ttccggcagc
    13381 ggcggcgaca tcatcaattt tgatctcctt gccccacagc tcaaccggcg taccaatccc
    13441 cgcctgcggg caaggcgtta aatcaaccgc cagcatatcc atcgagacgg tccccaccgt
    13501 catggtgcgc acgccgtcca ctaaaacagg agtaccggta ggcgcatggc gcggataacc
    13561 gtcggcgtat cctgcggcga caatgccgat tcgctgttca tcgcgcgcag tatagcgacc
    13621 gccgtagccc acacgctcgc ccgcttttag cgtctggaca ccaataatct cactgcttag
    13681 cgtcatcacc ggacgtaatc cggtattggc gatatcacgc cactgaccgg acggcgaagc
    13741 gccatacaaa ataatgccag gccgaaccca gtcaaaatgc gcttccgggt gccacagagt
    13801 cgccgccgaa ttggacaacg aacgccgaca ctccagcccc tccgccgcct gctcaatacg
    13861 cgccatcgcg ccggaaattc catcaggatg ttccgcttca gcaaaatgcg acatcagggt
    13921 catttcgcca acattcgcca ttgcccgcaa ctgctgccag acggtaagca cgcgatcgga
    13981 ctggaagccc aaccgattca tcccactgtt cactttaaga taaatatcca acggtgcttt
    14041 tagccgcgca ttttgcagtg ctttgagctg ccagttgctg tgtacgcagg tggtcaggcg
    14101 gtgctggtca taaatctcca gatcctgagc atggaaaaat ccttccagca tcaggatcgg
    14161 ccccttccag ccgcgctcac gtaacgttat tgcctcttcc aggttaagta atgcaaagcc
    14221 atcggtggcc ccgagcgcgc tccagatacg ctcaatacca tgcccgtaag cgttcgcttt
    14281 taccaccgac cagacgcgcg cgtgcggcgc ggcctggcgg acaatggaca gattctgttt
    14341 taatgcctgc agatcgaggc tggcctgtat cggacgggtc atctcgtttc cttagctgtg
    14401 tgcgccatgt aaatggcccg gacgtgatgg cgtaaatcca cggctgtagc gcgctacgct
    14461 tagatcctca tatgggatcg ctggcgtgcg accagagagc agatcgctta acaactgacc
    14521 ggaaccgcaa gccatcgtcc agccgagcgt gccgtgaccg gtattcagcc acagattttt
    14581 aaagcgtgta ctcccgacaa ccggcgtacc gtctggcgtc atcgggcgca aacccgtcca
    14641 gaaagtcgcc tgctcgacat gaccgccgcg tggatagaga tcgcgaacca ccatttccag
    14701 cgtttcacga cgcggttgca acagctcggt gttaaaacca acaatttccg ccattccgcc
    14761 aacacgaatg cggttatcga aacgggtaat ggcgattttg taggtttcat caagaatggt
    14821 ggatactggc gcaccatctt cctgcgcaat tggaatagtc agcgagtagc ctttcagcgg
    14881 atacaccgga atatcaacaa tgcctttgag catcgccgtc gagtaagaac caaacgccat
    14941 cacatacgca tcggccttaa tcacttcatc gccaaacttc acgccgtaga tttgctcgcc
    15001 gtcgcaaagc agttggtcaa cgggcgtatt aaagcggaat ttaacccccg cctgctctgc
    15061 catccgcgcc agattctggg taaacagctg gcagtctccg gtttcatcat tgggtaactg
    15121 caggccgccc gtcagtttgt gcgctacttc tgccagcgcg ggatccactt ccgccaggcg
    15181 gctggattcc agcagctgat acggtacgcc ggcatcttcc agcacggcga tatcgcgggt
    15241 cgcattttca tactgttgtt cggtacggaa cagttgcagc gtcccaccct gacgcccttc
    15301 atactgaata ttggtttcgg cgcgcaatgc tttcaggcaa tcacggctgt attccgccag
    15361 acgcaccatc cgccctttgt tttccatgta gtggctggtg tcgcagttac gtaacatttg
    15421 ccacatccat ttcaactgaa actgcgtacc gtcgagacga accgccagcg gcgcatggcg
    15481 ctggaacatc catttaatcg cttttaaagg cacacctggt gctgcccacg gtgcagcata
    15541 tccgggggag atttgcccgg cattcgcggc actggtttcc agggcggccc ccggctcccg
    15601 atcaatgacg gtgacctcat gtcctgcctg atttaagtac caggcgctgg caacgcctac
    15661 cacaccactt cccagtatga caactcgcat agccactccg ataacggtag atacagtaaa
    15721 agaataataa tctaattaca tcttgataac tcagttgaaa atattattca acatacggct
    15781 tttttatggt gagctgactc acatctccct gactctgcgg caatatggcg tctttagtat
    15841 ctcctgctga acgccctttt tatccagcat aaaattccgt tcagaagcgg attagtggca
    15901 ctctgataat aagaaatcgc aaagaaaaaa attctccggc gacgcggttt ttaacaggtg
    15961 ttctatgctt gaaatgaggt gctccacaca gagagcgccg acaacaatga ggtgcgcgta
    16021 tggcgacgat cgattctatg aataaggaca ccacacgttt gagcgatgga cccgactgga
    16081 cgttcgacct gctggatgtt tatctggcag agatagaccg ggtggcgaaa ctctaccggc
    16141 tggataccta cccgcaccag attgaagtga taacctcaga acagatgatg gatgcctatt
    16201 ccagcgtcgg catgccaatt aactatccgc actggtcatt cggtaaaaag tttatcgaga
    16261 ctgaacggct gtataagcac ggtcagcaag ggctggccta tgaaatcgtc attaactcta
    16321 acccgtgtat cgcttacttg atggaagaga acaccattac tatgcaggcg ctggtgatgg
    16381 cacacgcctg ctatggacat aactctttct ttaaaaacaa ttgcttattc cgcagttgga
    16441 ccgacgccag ttcgattgtc gattatctga tttttgcccg taaatatatt accgagtgcg
    16501 aagaacgtta tggcgttgat gaagtagaac ggcttctgga ctcgtgccac gcgctgatga
    16561 actacggcgt ggaccggtac aaacgcccgc aaaaaatctc gctgcaagaa gagaaagccc
    16621 ggcagaaaag tcgcgaagag tatctacaaa gtcaggtcaa tatgctctgg cgtaccctgc
    16681 cgaagcgcga ggaagagaaa acggttgctg aagcgcgccg ctatccgtcc gaaccacaag
    16741 aaaacctgct ctattttatg gagaaaaatg cgccactgct ggaatcatgg cagcgtgaaa
    16801 tcctgcgtat tgtgcgtaag gtgagccagt atttttatcc gcaaaaacag actcaggtga
    16861 tgaacgaagg ctgtgcgacc ttctggcact ataccatcct taaccatctg tatgatgaag
    16921 ggaaagtaac ggaacgtttt atgctggagt ttttgcacag ccacaccaat gtggtcttcc
    16981 agccccccta taacagcccg tggtacagcg gcatcaaccc gtatgccctc gggttcgcca
    17041 tgttccagga tattaaacgg atttgtcagt cgccaacgga agaagacaaa tactggttcc
    17101 cggatatcgc cggttccgac tggctggaaa cgctgcattt cgcgatgcgt gatttcaaag
    17161 atgagagttt tatcagccag tttctgtcac cgaaagtgat gcgtgatttc cgcttcttca
    17221 ccgtgctgga tgatgatcgg cataattatc tggagatttc cgctattcat aatgaagaag
    17281 gttatcggga gatccgtaac cggttatcgt cgcaatataa cttaagtaat ctggagccga
    17341 atattcagat ctggaacgtg gatttgcgcg gcgaccgttc gctgacgctg cgttatattc
    17401 cacataatcg cgcaccgctg gatcgggggc gcaaagaagt cctgaagcat gtgcatcgcc
    17461 tgtggggatt tgatgtgatg ctcgaacagc aaaacgaaga cggcagcatc gagttgctgg
    17521 aacgttgccc gccaagaatg ggaaatctgt aaaaacaaca aaaaacccct cgtttgaggg
    17581 ggttgctctt taaacggaag ggattatcgc ccctgaatgg ctaaatcacc cggcagattt
    17641 ttctgcatcc ggtgccaaat ctcgccactc tcatgcccat agcgacgcac tgtttcgtac
    17701 acctgatcgt gcgcgccttc actgcacaac gccgacagtt tgtggtagaa gcccagcgcc
    17761 agactgcgcg cttccggatt ggcgaaatag tgacgaccaa tgcgcgtata cagccctttc
    17821 atcccgttaa ggatcagacc gtaaatcggg ttgccggaag caaacgccag gccgcggaat
    17881 atgttgtaat ccagctcggc aaaggcatcg gcgtgatcgg ccacttcatt agcggtagcc
    17941 agcacttcct gcgctttatc gggatgctga cgaaacgcgg tgcgaataaa aatagtggaa
    18001 atattggtac gcaccgacag caaattatca ataagctgcg gcacactttc gtgatccagt
    18061 cgcgccagtg tttcaaggat atttaaaccg gaagtttccc agaaattatt caccttcgtc
    18121 ggcttgccat gttgaatggt caaccagcca tctcgtgcca gacgctgtaa cacttcacgt
    18181 aacgtagtac gcgttacgcc aattaattct gaaagttcac gttctgcggg caaaatagtc
    18241 ccgggaggga agcggttatt ccagatactt tcaataatgt actcttccgc gaaacccgcc
    18301 gggctttgcg ccttaatgac catagtgaga tttccataac acagcaaaac aaagttggac
    18361 tcatcatacc agagggctaa ctacgctgat agcagacaaa aagaagaaaa agggatcaag
    18421 gcttcatttt ccgcatctct ttccccttga ctatcaagac ttgcctcact cctgcctgac
    18481 cgttaagctt tgcgtttaaa cataaaaaca tcattgatta tttgtaaggt aagggaaacc
    18541 atcatggaga tctcctgggg ccgcgcgcta tggcgcaatt ttttgggcca gtcccccgac
    18601 tggtacaaac tcgccctcat cattttctta atcgtaaacc cgttaatttt cctcatcagc
    18661 cctttcgtcg cgggctggtt gctggtagcg gaatttattt tcactctggc gatggccctg
    18721 aaatgctacc cgctactccc cggtggtctg ttggctatcg aagcggtatt catcggcatg
    18781 accagcgcgg aacacgtccg tgaagaggtg gcggcaaatc ttgaagtctt gctgttattg
    18841 atgtttatgg tggcgggtat ctattttatg aaacagctgt tgctgttcat atttacccgt
    18901 ttgctgttaa gcattcgctc caaaatgctg ctgtcgctct ctttttgcgt ggcggcagcg
    18961 ttcctctccg cgttcctcga tgccttaacc gtcgtggcgg tggtgatcag cgttgcagtc
    19021 ggtttttatg gtatttatca tcgcgtagcc tcttcccgta ccgaagacac cgacctgcaa
    19081 gacgatagtc atatcgacaa gcattacaac gtggttctgg aacagttccg tggctttctg
    19141 cgtagcctga tgatgcatgc cggtgtcggc accgcattag gcggcgtaat gaccatggtg
    19201 ggcgaaccac agaacctgat catcgctaaa gcggctggct ggcattttgg cgatttcttc
    19261 ctgcgcatgt cgccggtgac cgttccggtt ctgatttgtg gcctgttaac ctgcctgctg
    19321 gtagagaagc tgcgttggtt tggctacggt gaaacgctgc cggagaaagt ccgcgaagtg
    19381 ttgcaacagt ttgacgatca aagccgccac cagcgtaccc gtcaggataa aatccgtctg
    19441 attgtccagg cgattattgg cgtctggctg gtgactgcgc tggcgttgca tctggcggaa
    19501 gttggcttga ttggtttgtc agtcattatt ctggcaacat cattgaccgg tgtcaccgat
    19561 gaacatgcta tcggtaaagc cttcaccgaa tctctgccat tcaccgcact gttgacggtg
    19621 tttttctcgg tagtcgcggt gattatcgac caacaactgt tttcgccgat tattcagttt
    19681 gtgttgcagg catctgaaca tgctcagctg tcgctgttct atattttcaa cggtctgctg
    19741 tcatccattt cggataacgt cttcgtgggg acgatttata tcaacgaagc gaaagcggca
    19801 atggaaagtg gcgctatcac gttgaagcaa tacgagctgc tggcggtcgc cattaatacc
    19861 ggtaccaatc tgccctctgt cgctacgccg aacggtcagg ctgcgttcct gttcctgctg
    19921 acctctgcac tcgcgccatt gattcgcctc tcttatggcc gcatggtgtg gatggctctg
    19981 ccttacaccc tcgtactgac actcgtcggc ttgctctgcg tcgagtttac gcttgcccct
    20041 gtaaccgaat ggtttatgca aatgggctgg atagcaacgc tttgataaca acttaccggg
    20101 catataaatg cccggtttgc cttttcgccc gataattgtc caattgcgtc ctatttttgc
    20161 tgcctcctgg tggcgcagcg aatgaattgg tttaaactgc gcactctatg catattgcag
    20221 ggaaatgatt atgttgcgat ttttgaacca atgttcacaa ggccggggcg cgtggctgtt
    20281 gatggcgttt actgctctgg cactggaact gacggcgctg tggttccagc atgtgatgtt
    20341 actgaaacct tgcgtgctct gtatttatga acgctgcgcg ttattcggcg ttctgggtgc
    20401 tgcgctgatt ggcgcgatcg ccccgaaaac tccgctgcgt tatgtagcga tggttatctg
    20461 gttgtatagt gcgttccgcg gtgtgcagtt aacttacgag cacaccatgc ttcagctcta
    20521 tccttcgcca tttgccacct gtgattttat ggttcgtttc ccggaatggc tgccgctgga
    20581 taaatgggtg ccgcaagtgt ttgtcgcctc tggcgattgc gccgagcgtc agtgggattt
    20641 tttaggtatg gaaatgccgc agtggctgct cggtattttt atcgcttacc tgattgtcgc
    20701 ggtgctggtg gtgatttccc agccgtttaa agcgaaaaaa cgtgatctgt tcggtcgcta
    20761 atccattcgg cgctcctgcg ggagcgcttt tttcctgccg ctatatttat ttgacccgca
    20821 gtaaatcaga acttcgcgtt gtataacgtg gtgaaagcat agctcgcttc atctgccatt
    20881 gttgttggat cccctgcccg gcaaaataga gtgttcctct gccctcttta gcattcagag
    20941 tatccattac cgccatcaat tgctcactcc cggggcgcgg tgcgttgtca tcgaataaat
    21001 tgagctgcgc gactccctga ctgaagaaat cccccagcat cacgcccgct ttttggtagc
    21061 gatggcccgc ttgccagatg gcatccagag atcgcgtagc agcgttaatg atatccctgc
    21121 tgtcctgagt gggcgtcagc agttttaccg acgcgctatt gccgtaataa ggttcattga
    21181 gcgcaaatgg tgacgtctta ataaatgtgg agataaaccg acaatattga tgctcactgc
    21241 gaagtttttc cgccgcccgg gcagcgtaac tacaaatggc ctgccgcatc gacgtataat
    21301 ccgtgatgcg ttcaccaaac gagcgggaac agataatttc ctgcttcgtc ggtgcaaact
    21361 cttccagttg caaacagggt tcgccgcgca gttcacgcac cgttctttcg agcatgacat
    21421 taaaatgttt acggataaac cggatatctg tatccgccaa atcgagaacg gttttgatcc
    21481 ccatcgcgtc cagttttttg ctgatccgcc gtccaatccc ccagacgtca tccacgggga
    21541 gagcagacat taatttacgc tggcgttcca gatttgataa atccaccacc ccacccgtct
    21601 gccgctgcca tttttttgcc gcatgattgg caagcttagc caacgttttg gtctgggcga
    21661 tccccacacc aacagtaaga tgggtacgtt gtagcaccgt tgcgcgaatt tctctgccaa
    21721 aatcagtcag atcgcgacaa ttacgcacac ctgtcagatc gcagaatgct tcatcaatac
    21781 tgtaaatctc gacgcggggc gatagttctt ccagcgtcga catcacccga ttgctcatgt
    21841 ctgcgtaaag ctcataattg ctgctaaagc aaaccacacc acagcgacga aacagatctt
    21901 tttgtttgaa ccagggatcg cccattttaa cgcccagcgc ctttgcctca gcgtttcggg
    21961 cgataacgca accgtcatta ttcgatagca caaccaccgg tttaccccat aaatcagggc
    22021 gaaacaccgt ctcacagctg gcataaaacg cgtttacatc acagagggca aacatcagcg
    22081 catcgcctta acgacgtgga tcaccacacc aaagacatcc agcgtatctt cgctactgat
    22141 ggtaatgggc gagtacgcac tgttcatggg aataagctgt accgtcgggc gtagttgcaa
    22201 ttttttcacc gtaaactcgc cgtcaacagc agcgatgaca atatcaccat ggctggcggt
    22261 aatagcgcta tcgacaatca gtaaatcacc gtcactaatt ccaccatcaa tcatagaatc
    22321 accacttgct ttgacgaagt aagtcgcgct gggatgctgg atcaacagtt gattcagatc
    22381 gatgcgctgt tcaacgtaat ctgctgccgg tgaaggaaag ccacactgaa caagatcgct
    22441 aaatagcgga aaagtcacaa tttcgcggag atccgcaggc ttgataaaca acataataat
    22501 ctgcctgaag ttatactgtt tttatataca gtggtctgtt cttgccagca gatcaatact
    22561 gattcaggct atcaatattt gtcgctgcat agactgctga tttttcgttc tcttatcctg
    22621 tgctcacgtg gccttctggc gacgacgctc atccagcaga aatgaaaaat atcacccggc
    22681 aaaaaaatag aatagaagca ttcgccataa tgacattaaa cattgtttga tatttatcat
    22741 attaatagaa ataaagacat tgacgcatcc cgcccggcta actatgaatt agatgaagta
    22801 aaatttatta atagttgtaa aacaggagtt tcattacaat ttatatattt aaagaggcga
    22861 atgattatga ctgaaatcgt tgcagataaa acggtagaag tagttaaaaa cgcaatcgaa
    22921 accgcagatg gagcattaga tctttataat aaatatctcg atcaggtcat cccctggcag
    22981 acctttgatg aaaccataaa agagttaagt cgctttaaac aggagtattc acaggcagcc
    23041 tccgttttag tcggcgatat taaaacctta cttatggata gccaggataa gtattttgaa
    23101 gcaacccaaa cagtgtatga atggtgtggt gttgcgacgc aattgctcgc agcgtatatt
    23161 ttgctatttg atgagtacaa tgagaagaaa gcatccgccc ctcattaagg tactggatga
    23221 cggcatcacg aagctgaatg aagcgcaaaa atccctgctg gtaagctcac aaagtttcaa
    23281 caacgcttcc gggaaactgc tggcgttaga tagccagtta accaatgatt tttcagaaaa
    23341 aagcagctat ttccagtcac aggtagataa aatcaggaag gaagcgtatg ccggtgccgc
    23401 agccgatgtc gtcgccggtc catttggttt aatcatttcc tattctattg ctgcgggcgt
    23461 agttgaaggg aaactgattc cagaattgaa gaacaagtta aaatctgtgc agagtttctt
    23521 taccaccctg tctaacacgg ttaaacaagc gaataaagat atcgatgccg ccaaattgaa
    23581 attaaccacc gaaatagccg ccatcgggga gataaaaacg gaaactgaaa caaccagatt
    23641 ctatgttgat tatgatgatt taatgctttc tttgctaaaa gcagcggcca aaaaaatgat
    23701 taacacctgt aatgagtatc agaaaagaca cggtaaaaag acactctttg aagtacctga
    23761 agtctgataa gcgattattc tctccatgta ctcaaggtat aaggtttatc acatttaaaa
    23821 acacagacgt tttttaaaga ttgagagtta cctgttgata ataaggtatc gggtaactct
    23881 cattatgaca gagcgctttc aaaaacaggg ctacgaaatg gcgagcatcc agacgtatat
    23941 taagaaagcc cccatcagca tctattaaat ttatcactgc gcccagtcag gtttatttaa
    24001 gatatgatcc tgccagtcaa tcacttctga ttctttcact gcgatatgac gcacagagat
    24061 acgttcacca tgcattgccg ctttcgaacc agtaagtagc ggatgccacg caggtaaatc
    24121 tttaccttcc gccagcaacc gataagcgca ggtcattggc agccattcga atgttggcag
    24181 attttcacgg gttaatttaa tgcagtcggg ttcaaactcg aaacgacgtt cgtagttccg
    24241 acattgacag gttttaatat tgagctggcg acaggcgacg ttagtgaagt agatttcgtc
    24301 ggtgtcttca tccatcagtt tatgcaagca acactgaccg caaccatcac acaacgactc
    24361 ccattccgca tcgctcattt cgtccagggt tttactttgc cagaaaggta catcgctcat
    24421 caggtgttcc gccattacgt taaaacgcac cttataacca gtctggcaca gcgatgcaag
    24481 ttttgccgcc gctttctggc ggcaaaaagt attacaaaac gcgagttgtc aaagaatgcc
    24541 catcgaaagt gactgtcagc tcatcaccgc tttgcaacgg gccgacgcca tcaggcgtgc
    24601 ctgtcaacac aacgtcaccg gccttgaggg taaaaaactt gctcatataa gcgatcaacg
    24661 gaacgatttt atggatcatg tccgcagtcg taccttgctg gcgtttttcg ccgtttacgc
    24721 tcaggcccag cgttgtattt tgtggatcgc cggtaaattc cgccgcgggg ataaacccgg
    24781 aaagcggaca agagttatca aacgctttag ccttttccca cggctgcccg gctttcttca
    24841 tttttccctg aacatcacgc aatgtcagat caagcgccac gccgtaaccg gcaatggctt
    24901 tgcggacatg ctcttccgtt gcctgacgca gcgtcgcgcc aatcaacacc gccagttcga
    24961 cttcatgatg aactgaaccg aaatcggatg ggatcgccaa tggctgccgc agatcgcaca
    25021 gtgccgtttc tggtttaata aacagcactg gctcttcggg cactgcgctg cccatctctt
    25081 taatatgttt ggcatagtta ctgccaacac agactacttt actcaccgga taatccagca
    25141 gcgcaccttg ccagttgtga tgttgataca taacgttccc ctcaccttat cgtggatatg
    25201 aaaacccgcg ccgacaaaga taatgccacc gccggatatc ggttatttgt tagtgtcgtc
    25261 tgttttctgc cccatgacgg aaagatgttg cttcagcaaa tcttcgggtg gtggcggtaa
    25321 ctgcaaatag taaccttgct cggttaatgc cagtttaacc ttttcaatat cagcattgac
    25381 cagtttcttc cgcccatcca acggcagaat catcgctaac tgaggctgac caaaaccttt
    25441 catcagttcc tcaggaacac gcgaaaaatc atc
//
LOCUS       DAJVPP010000060        23584 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVPP010000060 DAJVPP010000000
VERSION     DAJVPP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.060"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..678)
                     /gene="rluF"
                     /locus_tag="OQM34_002430"
     CDS             complement(<1..678)
                     /gene="rluF"
                     /locus_tag="OQM34_002430"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1452839.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTD"
     gene            1026..1973
                     /locus_tag="OQM34_002431"
     CDS             1026..1973
                     /locus_tag="OQM34_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCS1452840.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2043..2849
                     /locus_tag="OQM34_002432"
     CDS             2043..2849
                     /locus_tag="OQM34_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1452841.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            2859..3266
                     /locus_tag="OQM34_002433"
                     /pseudo
     CDS             2859..3266
                     /locus_tag="OQM34_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3266..3760
                     /locus_tag="OQM34_002434"
     CDS             3266..3760
                     /locus_tag="OQM34_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCS1452842.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            3826..4623
                     /locus_tag="OQM34_002435"
     CDS             3826..4623
                     /locus_tag="OQM34_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1452843.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            4634..5422
                     /locus_tag="OQM34_002436"
     CDS             4634..5422
                     /locus_tag="OQM34_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1452844.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5474..6703
                     /gene="sorE"
                     /locus_tag="OQM34_002437"
     CDS             5474..6703
                     /gene="sorE"
                     /locus_tag="OQM34_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCS1452845.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7085..7774
                     /gene="pepE"
                     /locus_tag="OQM34_002438"
     CDS             7085..7774
                     /gene="pepE"
                     /locus_tag="OQM34_002438"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCS1452846.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7865..9496)
                     /locus_tag="OQM34_002439"
     CDS             complement(7865..9496)
                     /locus_tag="OQM34_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1452847.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9716..13399)
                     /gene="metH"
                     /locus_tag="OQM34_002440"
     CDS             complement(9716..13399)
                     /gene="metH"
                     /locus_tag="OQM34_002440"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1452848.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGVDIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDLNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQSKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLRNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13599..14423
                     /gene="iclR"
                     /locus_tag="OQM34_002441"
     CDS             13599..14423
                     /gene="iclR"
                     /locus_tag="OQM34_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1452849.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            14740..16924
                     /gene="espL4"
                     /locus_tag="OQM34_002442"
                     /pseudo
     CDS             14740..16924
                     /gene="espL4"
                     /locus_tag="OQM34_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(16893..18630)
                     /gene="aceK"
                     /locus_tag="OQM34_002443"
                     /pseudo
     CDS             complement(16893..18630)
                     /gene="aceK"
                     /locus_tag="OQM34_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(18814..20118)
                     /gene="aceA"
                     /locus_tag="OQM34_002444"
     CDS             complement(18814..20118)
                     /gene="aceA"
                     /locus_tag="OQM34_002444"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCS1452850.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20148..21749)
                     /gene="aceB"
                     /locus_tag="OQM34_002445"
                     /pseudo
     CDS             complement(20148..21749)
                     /gene="aceB"
                     /locus_tag="OQM34_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22018..22947)
                     /gene="metA"
                     /locus_tag="OQM34_002446"
     CDS             complement(22018..22947)
                     /gene="metA"
                     /locus_tag="OQM34_002446"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1452851.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23104..23547
                     /locus_tag="OQM34_002447"
     CDS             23104..23547
                     /locus_tag="OQM34_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS1452852.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5824 a         6018 c         5982 g         5760 t
ORIGIN      
        1 gtcggttaaa tcgcgccact cccccagcgg aatgccgctt aagctgacgt tcataatgcg
       61 cgtacgttcc agctttttca cttcatagcc gaaatgctcg cacatgcgcc ggatctgacg
      121 gttcagcccc tgcactaggg taatgcggaa gacaaacggc gcttcttttt tgactttgca
      181 ctttttcgtc accgtcccga ggatcggcac ccccgccccc ataccacgaa taaattcatc
      241 ggtaatcggt ttatcgaccg tcaccagata ctctttctca tgatcgttac ccgcacggag
      301 gatcttattc accagatcgc cgtgattggt gaggaaaatc agcccctggg agtctttatc
      361 caggcggcca atcgggaaca cgcgtttgct gtggttaacg aaatcgacaa tgttatcgcg
      421 ctcgccatct tcggtggtgc tgacaatacc gacgggcttg ttcagggcga taagtaccaa
      481 atcttcggct tcccgaggct caatcaactg accgtttact ttcacaatgt cgccgggttt
      541 cacctgatcg ccaatggtgg ctcgcttgcc attaaggaac acattgcctt gctcgatata
      601 gcgatccgct tcgcggcgtg agcaaattcc gctttcgctg atgtatttat ttaaacggac
      661 tgatgagtcg ggcagcataa attctcctgt aaaacgcgga atataccgta cctcttagcg
      721 gggaaaaaca gggttatgcg gcaacgtcga aattaaatgt gatctgccac gccttttatc
      781 acaaaagtgt ctccggaagt acgatgcaaa tttgcctggc aaaataagga taatcaacat
      841 attacgaatc gctgcacacc tgtgctgatg actactacgc agagaaacaa atgtgcaaga
      901 gaaagcgcta tttctcccca tgcaaagagt gaaaaatcgc tttgaaaggc cctcatgatg
      961 gggttatatc agatcaagcg gtgagaaagc gtatttgcac aaatgtgcag aaccaggagg
     1021 cggcaatgga aaacagtgac gatatccgtt tgattgtgaa gattgcccaa ctctattacg
     1081 aacaggatat gacgcaggcg caaatcgcgc gcgaactggg tatttaccgc accaccatca
     1141 gccgcttgct taaacgaggc cgcgatcagg gaatggtcac catcgccatc aactatgact
     1201 acaacgaaaa tctctggctg gagcagcaac tgaagcaaaa gtttggcctg aaagacgttg
     1261 tggtggtgtc gggaaatgat gaggatgaag agactcaact ggcgatgatg gggttacacg
     1321 gcgcgcaact gctggatcgc ttgctggagc ctggcgatat tgtcggtttt tcctggggtc
     1381 gcgcggtgag cgcactggtt gaaaacttgc cgcaggcggg gcaatcgcgg cagttaatct
     1441 gcgtaccaat tattggcgga ccgtccggta aactcgaaag ccgctatcat gtaaacacat
     1501 taacctacag cgcggcagcg aagctgaaag gggaatcgca tctcgcggat tttccggctc
     1561 tgctggataa cccattaatt cgtaatggga tcatgcagtc tcagcacttt aaaaccatct
     1621 ctgcctactg ggataatctg gatatcgccc tggtgggaat tggctcaccg gccattcgcg
     1681 acggcgctaa ctggcatgcg ttttatggtg gtgaagagag tgacgacctg aatgcccgcc
     1741 aggttgctgg cgatatttgc tcgcgctttt ttgatattca cggcgcaatg gttgaaacga
     1801 atatgagcga aaaaacgctc tctatcgaaa tgaataaatt aaagcaggcg cgatattcca
     1861 ttggcattgc catgagtgaa gaaaaataca gcggaattat tggtgcactg cgtggaaaat
     1921 atattaattg tctggtaacg aatagcagca cagctgaact attactgaaa taaaaatctt
     1981 cccgatgttt accgtaataa aaattacggc gggtccctat tatctaaaat caggagtctg
     2041 ttatgcaaac gtggttaaat ttgcaggata aaatcattat tgtcaccggc ggcgcatccg
     2101 gtattggtct ggcgattgta gaggaattat tagcacaagg cgcgaatgta cagatggtcg
     2161 atattcacgg tggcgatggt caatatgaag gtcataaagg ctatcagttt tggccgaccg
     2221 atatttctag cacaaaagag gtaaatcata cggtagcgga aattatccag cgttttggtc
     2281 gcatcgacgg tctggtcaat aacgccgggg tcaatttccc gcgtctgctg gtcgatgaga
     2341 aagcgcctgc cgggcagtat gaactcaacg aagctgcatt cgaaaaaatg gtcaatatca
     2401 accagaaagg tgtttttctg atgtcgcagg cggtggcgcg acagatggtc aaacaacatg
     2461 atggcgtgat tgtgaatgtt tcctcagaaa gtgggctgga aggctcagaa ggacaaagct
     2521 gttacgccgc taccaaagcc gcgctcaata gcttcacgcg ctcctggtcg aaagagctgg
     2581 gtaaacacgg tatccgtgtg gtcggtatcg cgcccgggat tctggaaaaa accgggctac
     2641 gcacgccgga atatgaagag gcgctggcgt ggacgcgcaa tatcaccgtc gagcagctgc
     2701 gtgaaggcta taccaaaaac gccattccta ttgggcgcgc cggaagatta gcagaagtag
     2761 ctgattttgt ttgttatctg ttgtctgaac gcgccagcta catcaccgga gtaaccacta
     2821 acattgcggg cggcaaaacg cgcggctaag gaggctttat ggtcaacgcc attttttgtg
     2881 cccacggcaa attagcttgc gccatgctgg aatcggttca gatggtttac ggcaacgcca
     2941 atgttgaagc cgttgcattt gtgcccggtg aaaacgccgg agacattgcg gtaaagctgg
     3001 aaaagttagt aagcattcac aatcaagatg agtggctgat tgccgtggat ttacagtgcg
     3061 gcagcccgta gaatgcggca gcaatgcttg cgatgcgcaa tcctcgtttg cgggtaatca
     3121 gcggcctttc tctgccgctg gcgctcgaac tggttgataa ccaggacagc atgaatgttg
     3181 atgaactgtg tgaacacctg acgcaaatcg caaagcaaac ctgcgttgtc tggaaacaac
     3241 tggcaacgac agaggaggat ttctgatgaa cattacgctc gcccgcattg atgaccgtct
     3301 gatccacggc caggtcacta ccgtctggtc gaaagtggct aacgcccagc gcattattat
     3361 ctgcaatgac gaagtttata acgatgaagt gcgccggaca ttattgcgtc aggctgcgcc
     3421 accgggaatg aaggttaacg tggtcaatat tgaaaaagcc gtcgcggttt atcacaaccc
     3481 gcaatatcag gacgaaaccg ttttttatct tttcactcgc ccacaggatg ccttagcgat
     3541 ggttcgccag ggggtaaaaa taggcaccct gaatattggc ggcatggcct ggcggcccgg
     3601 taaaaaacag ttaaccaaag cggtttcatt agatgatgat gatattaacg catttcatga
     3661 gttaaataat cttggtgtca tactggattt acgcgtcgta gcgtcagatc cctcaatcaa
     3721 tattatcgac aaaataaacg aacagttaat cgcaaattaa ataatagcgc ctgtgtacat
     3781 atgctttaac aggcagggat gacgacactt aaaaggtgac aaatcatgga aataagtacc
     3841 ctacaaatca ttgctatatt tcttttttcc tgtattgccg gaatgggcag cgtgctggat
     3901 gaatttcaga ctcatcgccc gttaattgcc tgtacggtga ttggtttaat tctcggtgat
     3961 ttaaaaaccg gaataatact cggcggtacg ctggaattga tagctctctg ctggatgaac
     4021 gtcggcgcgg cgcaatctcc ggattctgca ctcgccagca taatctccgc cattctggtt
     4081 atcgttggtc agcaaagcat cgccaccgga atcgccatcg cgttgcctgt ggctgcggca
     4141 ggccaggtgc tgacggtgtt tgcccgtacc atcaccgtag tgttccagca cgcggcggat
     4201 aaagcagcag aagaggcgcg gtttcgcact ctcgatattc tgcatgtctc cgcgcttggc
     4261 gtgcaggcgc tgcgcgtcgc cattccggca ctgattgtct cactgttcgt cagcgccgat
     4321 atggtcagca atatgttaaa cgccattccc gaatttgtga cccgaggact acagattgct
     4381 ggcggtttta tcgtggtggt cggttacgcc atggtgctac gtatgatggg cgtgaaatat
     4441 ttgatgcctt tctttttcct cggtttcctc gcaggtggct acctcgatct cagcctgctg
     4501 gcgttcggtg gcgtcggcgt gatcatggcc ctggtctaca tccagctaaa tccacagtgg
     4561 cgtaaagctg aacctcaact ccaggccacc gcctccaccg cccttgacca acttgacgat
     4621 taacggagcc atcatggaac agagaaaaat tacacgcagc gatctggtga gcatgtttct
     4681 gcgctccaac ctgcaacagg cgtcctttaa ctttgaacgt attcacgggc tgggcttttg
     4741 ctacgacatg atccccgcca tcaagcgact ttacccatta aaagaggatc aggttgcggc
     4801 gctcaggcta cacctggtgt tcttcaatac cacgccagcc gtatgtggcc cggtcatcgg
     4861 cgcggaaatt gatgacggta ccatcaacgg catcaaagtc ggtctgatgg gaccattggc
     4921 aggagttggc gatccactgg tctggggaac gctgcgcccg attaccgccg cgctcggcgc
     4981 atctctggca ctttcgggca acattctcgg cccgctgctg ttcttcttta ttttcaacgc
     5041 ggtgcgtctg gcgatgaagt ggtatggcct acagctcggc tttcgcaaag gggtgaatat
     5101 cgtcagcgat atgggcggga atgtgctgca aaaactcacc gaaggcgcgt cgattctcgg
     5161 actgtttgtg atgggcgtgc tggtgaccaa atggacgtca atcaacgtac cgttggtggt
     5221 ttcacaaacg catgccgccg atggctccac cgtcaccatg accgtgcaga acattctcga
     5281 ccaactttgc cctggtttgc tggcgctcgg tctgacgcta ctaatggttc gtctgctcaa
     5341 caaaaaaatt aacccggtat ggctgatttt cgccctgttt ggcttaggga ttatcggcaa
     5401 tgctctgggc ttcctgtcct gattcttcgc cccggcacga ctgccgaggc catcgctcaa
     5461 catgaggtgg tttatgaaaa caacagctct gcgtctttat ggtaaacgtg atttacgcct
     5521 ggaaaccttt gaccttcctg aaatgcagga ggatgaaatc ctcgcaacgg tggtcactga
     5581 cagcctgtgc ctctcttcct ggaaagaggc caatctgggt gaaaaccata aaaaagtacc
     5641 cgacgatgtg acgaccaacc caatcatcat cggccacgaa ttttgcggcg atattctggc
     5701 cgtgggtaaa aagtggcagc acaaattcca gccgggtcag cgttatgtga ttcaggccaa
     5761 cctgcaactc cccgaccgcc cggattgccc cggctactcc ttcccgtggg tagacggcga
     5821 agcgacgcat gtggttattc ccaacgaggt catggaacaa tattgcctgc tggcatacga
     5881 cggcgaaacc tattttgaag gctcactggt tgaaccgctt tcctgcgtga ttggcgcgtt
     5941 caacgccaac tatcatcttc aggaaggtag ttataaccac acgatgggga ttcgcccgca
     6001 agggcgcacg ctgatcctcg gcggcaccgg accaatggga ctgttggcga ttgattatgc
     6061 gctgcatgga ccagttaacc cgtcgctgct cgtcattacc gataccgaca acgacaaatt
     6121 gagttatgcg cgcaagcact atccatcaga accgcaaaca ctgattcatt atctcaatgc
     6181 caccgatgca gcatttgata cgttaatggc gctgagtggc ggtcacggct tcgatgatat
     6241 tttcgtcttt gtgcctaatg aaggactggt gactctcgcc tcttccttgc tggcgacaga
     6301 tggttgcctg aatttcttcg ccggaccgca ggataaacat ttcagcgcgc caattaattt
     6361 ctacgatgtg cattatgcat ttacccacta cgtgggcacg tcaggcggca ataccgacga
     6421 catgcgcgcg gcggtcaaac tgatcgaaga gaaaaaagtg caggccgcaa aagtggtaac
     6481 acatattctt gggctgaatg ccgcgggcga aaccacgctt gaattgcctg ccgtcggcgg
     6541 cggcaaaaag ctggtgtata ccgggaaata cctgccgctg acgtcactca cgcagattca
     6601 ggatcaagca ctggcggcga ttctggcgcg tcatcagggg atctggtccg gtgaggcgga
     6661 gcaatacctg ctcgcccatg cagaggcaat ttcccatgat taatcgcgct acttaactct
     6721 gcatgtcgct ggctggtcgc cccagcaact tcgcgaccgg atgtgcggct gattgctgaa
     6781 gcagcacaat ttgcacgaga aggataaaaa atatcagcgc cttaaggggc gctcaaattg
     6841 gtggcaaagt acacattgtt tatgccggat gcggaatgaa gccttattcg gtctacataa
     6901 ttttgaaaat ccaatatatt gcagttatct ggtaggccag gtaagcgtag cgcatcaggc
     6961 aatgctgtgt ttgtcatcag tctcagcgcc cggtgcaata cgtgacatcg tgagccatgt
     7021 cagtaagtgg ttaaattcgc agtggtagca tgccttccgt gatttcttac taaccggaga
     7081 agtaatggaa ctgcttttat tgagtaactc gacgctgccg ggtaaagcct ggctggaaca
     7141 tgcactgccg ctaattgctg aacagttgca gggtcgccgc tcagcggtgt ttatcccttt
     7201 cgctggcgta acgcagacct gggatgatta cacagagaaa acggctgcgg ttctcgctcc
     7261 gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat cccgttgccg cgattgaaaa
     7321 tgcggaaatt gtgattgtcg gcggcggtaa tactttccag ttgctgaaac agtgccgcga
     7381 gcgcgggctg ctggctccaa ttactgacgt ggttaaacgt ggcgcgctgt atattggctg
     7441 gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata tgccgattgt
     7501 cgatccgcaa ggtttcgatg cgctaaatct gttcccgttg caaatcaacc cgcacttcac
     7561 caacgcgctg ccggaaggcc ataaaggtga aacccgtgag cagcgtattc gcgaactgct
     7621 ggtcgtcgcg ccagaactga cgattattgg tctgccggaa ggtaactgga tcacagtgag
     7681 taaaggtcac gctacgctgg gtggcccgaa caccacttat gtgtttaagg ctggtgaaga
     7741 agcggttccg ctggaagctg gtcaccgttt ttaagtgaat tgccggatgc atgatccggc
     7801 atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta caggattcac
     7861 aatattaata ctcgtcccgt ccttcatctt catccggctg ttccagcaca ctgtaagcca
     7921 ccgaacaaaa cagcgagttc aggcgctgca tatctcccag taagcctaaa tgtagcgaac
     7981 tggtttcaat gctctgcacg ttttgctgat gcaggcgatc gacgtgggcg tgggaatagc
     8041 ggcgattaag aatgcgaaaa cgatgtttgc tgcgacgcaa acgacgagcg ctggtgacat
     8101 cgccagagaa gaacaccgac attgccagct ttaaattact gagcaattgc tcatagagcg
     8161 catccagttc cttcaaccca tcaagcgaaa atgcccgccg tgctgccagc gatttatcgg
     8221 caatttcgct gcccatgcgc tcgacgatat cggaggcctg ttcaaggttg agcgacattt
     8281 cgatgatctc cgcccagcgg cgcgactctt cctccgccaa ctcttctttt ggcatccgcg
     8341 ccagatacag cttaatggcg gtgtagagaa cgttgatatc atctgccagc ttacgcagct
     8401 ctttctcctg ccgtggctcg ccgtgcatca ctttattcag cccttccatc atctgttcca
     8461 tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc cagagcaagc gtgggcgtgt
     8521 ccagcgcgct gacatccaga tgtttaggcc gtagctgggt atccagttcc ggttcatcgc
     8581 gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg accaggcaac
     8641 gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc aatgacagct
     8701 tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta cccaccagct
     8761 taaacagcag actacccagc gcgacacggc gggctgcggc attggcggca ctgttgttga
     8821 gcatcgccag caggccggaa ccgaggttag ctccaatcac cagacagagc gccacgggga
     8881 aggagataat gcctgcagcg gtcagagtcg cggtcagcag tacagcagca aggctggagt
     8941 agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg ccggtcagcg
     9001 aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc tgcacaatca
     9061 actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt tgcccggcgc
     9121 gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac ggtgacagcc
     9181 aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct gcccccagca
     9241 caatgaccag agccggtgcg agggctacca gatcctgagc gacgaacgag gtcaccagca
     9301 tggtggtggc attactgctc tgtaccagtg cggtaacgcc gatccccgcg caaaaggcga
     9361 gcggcttttt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg aagacgcgca
     9421 ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca gaaagcaggt
     9481 gaagcagcgt taacacgagt tcaggttctc cttatcgtta tatgttcctg aagcaattga
     9541 atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt gacattcgtt
     9601 tttccggcta agattattcc agcctgtttc cagtataagt gtttcatatt aataaagaga
     9661 cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt gtgaatcagt
     9721 ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atcggagacg ctcatacctt
     9781 tacggcgggc atagtcttca acctgatcgc gctgaatttg cgccaccgcg tagtacttgc
     9841 tgtccggatg actgaaatac caacccgaaa ccgatgcacc aggccacatc gcgaaagatt
     9901 ctgtgagttt catgccagta tgtttttcca cttccagcag ctcccagatg gtggcttttt
     9961 ccgtatgttc cgggcaggcc ggatagcccg gtgccggacg gatgccctgg tagttttcgc
    10021 ggatcagttc ttcgtttctg aggttctcgt tcggcgcata gccccagtag actttacgca
    10081 cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc gctttcacca
    10141 tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg tcctcttcca
    10201 gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc ttcggcgcaa
    10261 cgaagtcagc gagacagtag ttagcgaatc ccgttttttc ggtctgttga cgcagatggt
    10321 ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg tcatcgccca
    10381 cacggtttgc cgggaacagg cccaccacgc cacgcggatt cagcgttttc tcggcgctta
    10441 atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg cccaccactt
    10501 catcttccag aatgcgcgga tacttcccgg ccagcgacca ggtcataaag aacggtgtcc
    10561 agtcgatgta attacgcagc gtttcgatgc tggcttccac ttcctgcacg ccgagacggt
    10621 gcgccaccgg cggcgtgtaa gcctgccagt caaatgcgaa atcgttatcg cgcgccgctt
    10681 ccagcgtgac cggtggtgtg cgtggtttct tgcgcccgtg ctgaatacgc acggtttcgt
    10741 actccttgca ggtacgagcg acaaaatcat cacgctgagt atcggaaagc agcgccgcta
    10801 ccacaccaac ggtgcgcgag gcattctgca catacaccgt agggccgctg tagttctgct
    10861 cgattttcac cgccgtgtgc gcttttgagg tggtcgcgcc gccaatcagt aacggaatgg
    10921 cgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc gacggcgtga
    10981 taagccccga aaggccaatc aaatcagcat tcacttcttt ggcgatacgg agaatttttt
    11041 ccgcaggcac cataacgccg agatcgacaa tttcgtagtt gttacattgc agcaccacac
    11101 caacgatatt tttaccgatg tcgtggacat cgcctttcac ggtggcgatc accatcttgc
    11161 cgttggtttt actctgctct ttgctggctt caataaacgg ttcgagatac gccactgcct
    11221 gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa cattttgcct tcgccaaaca
    11281 gatcgccgac cacgttcatg ccgtccatca acgggccttc aatcacttca atcgggcgcg
    11341 tagcctgctg gcgggcttct tcagtatcct gctcgataaa ctcggttatg cctttgacca
    11401 gcgagtattc cagacgttta ttcacgtccc acgagcgcca ctccgcttgc tgggcgttag
    11461 cagtgtcgtc cgctttactg ccgcgatatt tctcggcaag ctccagtaaa cgctcagtgc
    11521 catcgtcacg acgattaaga atcacatctt ccaccgcgtc gcgcagttca gcgggtaggt
    11581 cgtcgtaaat cgccagttgc ccggcgttga cgatccccat atccatgcca ttgcgaatag
    11641 cgtagtagag gaacactgcg tgaatggctt cgcgcaccgg atcgttgcca cggaatgaga
    11701 aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg cagttcgcgt ttgatgtctt
    11761 cacacgcgcc gataaagtcc tgcgcgtagt tgttgtgctc ttcaataccg gtggcgaccg
    11821 cgaagatgtt caggtcgaag atgatatctt ctggcgggaa gccaacctct tcggtgagga
    11881 ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg agtatcggcc tgtccctgtt
    11941 cgtcaaaggc catcaccacc actgccgcac cgtagcgacg caacaatttc gcgtgatgga
    12001 taaaggcgtc gacgccctct ttcatcgaga tagagttaac aatgcctttg ccctggatac
    12061 acttcagacc tttttcaatg acatcccatt ttgaggagtc gatcataatc ggcacgcgag
    12121 cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct tcggcatcga
    12181 gcatcccttc atccatgttg atatcgataa tctgcgcgcc gttttccacc tgctgacgcg
    12241 ccacatccag cgcctcgctg tatttctctt ctttgatcag gcgcttgaac ttagcagagc
    12301 cggtgacgtt ggtgcgttca cccacgttca caaacaggct atcgtcgcca atgttcagcg
    12361 gctctaggcc agacaaacgg caggctacgg gaatttccgg cagtttgcgc ggcgctaatc
    12421 cttctactgc acgactcatc gctgcaatat gttgcggcgt ggtcccgcag caaccaccga
    12481 cgatattgag aaagcccgct tgcgcccatt cacgtatctg ttttgccatc gtgtcggcgt
    12541 cgagatcgta ttcaccaaag gcgttaggca gcccggcgtt cgggtgggcg gtgacgtagc
    12601 attccgcaat ccgtgatagt tcctgcacgt actggcgcag ttcatcgggc cccagcgcac
    12661 agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga gttgtaaaat gcttcggtgg
    12721 tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt gccggagatc ataatcggca
    12781 gctcaacgcc cagcgcttca aactccgttt tcaccgcaaa gaccgccgct ttggcgttaa
    12841 gggtgtcgaa aacggtttca atcaggatca gatccgcacc accttccacc agcgctttgg
    12901 tggattcgcg ataggccgcc gccagctggt caaaagtgat gttgcggaat gccggatcgt
    12961 tgacgtccgg agaaatagac gccgtgcggt tggttgggcc aagaacaccg gcaacgtagc
    13021 gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca ggcgcgcgcc agtttcgccg
    13081 ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc gcaatggtcg
    13141 tggagttgaa ggtgttggtt tcgatgatat ccacgcccgc ttcaaagtag gcgttgtgga
    13201 tagcggcgat cacttccggt ttactgagta ccagcaggtc gttgttgcct ttgaggtcgc
    13261 atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga tagctctgga
    13321 tcatggtgcc cataccgccg tccaacacca gaatacgttc atttaactgc gcacgcagtt
    13381 gttccacttt gctgctcaca cttgctcccg acacgctcaa cccagattta ataaaaattc
    13441 aacaaaccat actggaataa acgcatctgt ggtaaaagcg accaccacgc aacatgagat
    13501 ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattagcta acaataaaaa
    13561 tgaaaataat ttccacgata cagaaaaagg aggctgtcat ggtcgcaccc attcccgcga
    13621 aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt cagtctttaa
    13681 cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagcgtg gcactcacag
    13741 agctggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta accacgatgc
    13801 aacagcaggg tttcgtgcgt caggtcggcg aactgggaca ctgggcaatc ggcgcacatg
    13861 cctttatggt cggcagcagc tttctccaga gccgtaattt gttagcaatt gttcacccta
    13921 tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg cttgatcaaa
    13981 gcgatcacga agcgattatc atcgaccagg tacagtgtac ccacctgatg cgaatgtccg
    14041 cgcctatcgg cggtaaattg ccgatgcacg cttccggtgc gggtaaagcc tttttagccc
    14101 aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat gcctataccc
    14161 acgcaactct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg cgcaaacggg
    14221 gttattcatt tgacgatgag gaacatgcgc tggggctacg ttgccttgca gcgtgtattt
    14281 tcgatgagca ccgcgaaccg tttgccgcaa tttctatttc cggaccgatt tcacgtatta
    14341 ccgatgatcg cgtgaccgag tttggcgcga tggtgattaa agcggcgaag gaagtgacgc
    14401 tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt ctgcccatca
    14461 tacctgagtg gcaatagaat aagggtgtct gttaatcgca ttgacgccaa aataacttaa
    14521 tgtcatacac ttcacacact tttattaact cagcattatt tttaaacatc aaaccactta
    14581 attattaaca aaatcgcaag atctcattcc accgaaactt ttacggctgc attatgagat
    14641 tgagcgattt atgatatttt tttagttgta atcatattaa attttttcac tgttctgata
    14701 gttaaaattc aagacatcaa taaacaatga gatatttaaa tgattactcg tattcctcgt
    14761 agttctttct ctgcaaatat taataataca gcccagacaa atgaacacca aaccctgagt
    14821 gaattgtttt ataaagaact cgaggataaa ttttctggca aggagctggc gacgcctcta
    14881 ttaaaaagct tctcagagaa ctgtagacaa aatgttcgtc atatctttag caacaaggat
    14941 tttgtcatta aattttccac gtccgtctta caagctgata agaaagaaat tacgataatt
    15001 aataaaaacg aaaacacgac actcactcaa accattgcta caatatttga agaataccta
    15061 atggaaattt tacctcaacg ctcagacact cttgataaac aagaattaaa cctaaaatca
    15121 gatagaaaag aaaaagaatt cccaagaatt aaacttaatg gtcaatgtta tttcccgggg
    15181 cgccccaaaa ccgtatagta taccgacaca ttgctgcaca atatattaat gatatttatc
    15241 agaatgttga ttacaaaccc catcaagatg attactcttc agctgaaaaa tttctcactc
    15301 acttcaacaa aaaatgcaaa aaccagactt tggcgttgat ttccagccgt cctgaggggc
    15361 gttgcgttgc tgcctgcggt gatttcgggc tagttatgaa agcatatttt gacaagatgg
    15421 aatcaaatgg catcagtgtt atggcagcca tattactggt ggataaccat gctttgacgg
    15481 tccggctaag aataaagaac acaactgaag gatgtaccca ttacgtggtt tcggtttatg
    15541 atcctaatgt aactaacgat aaaataagaa ttatgagcga aagcaaagag gatattaaac
    15601 actattctct gatggatttt atgaatgtag attatagcct cctgaaatgg tcaaatgatc
    15661 atgttattaa ccaatctgtt gcaataattc cagcacttcc gaaagaacag ctattgatgt
    15721 taaaaggaac tgtggatgaa ataacccctc cattatcacc agcaacgatg aatttgctaa
    15781 tggcaattgg tcagaatcac caacttacgc aactgatgat tcagctccag aaaatgccag
    15841 aactacatag aacagaaatg ttgactgcct ataatagtat taatctcccc ggtttatatt
    15901 tggctataaa ttatggcaat gcggatatcg ttgagactat tttcaattca ttgtcagaaa
    15961 caggatatga agggttactc tcgaaaaaaa atctcatgca tattctggag gcaaaagata
    16021 aaaatggttt ttctggatta tttttagcga tatcacgtaa ggataaaaat gttgtaacct
    16081 cgattctgaa cgtcttacct aaactggcca caacacatca tttagataat gaacaggtgt
    16141 ataaattcct gagtgccaaa aatagaacgt ccagccatgt tttataccat gttatggcga
    16201 atggtgatgc cgacatgctg aaaatttttt tggtcgcgtt acctttgtta attcgcacat
    16261 gtcatttgac taaagaacag gtactcgatc tcctgaaggc aaaggatttt tatggttgcc
    16321 caagactata cctggcgatg caaaatggac atagcgatat cgtgaaagtt attctcgaag
    16381 cattgccctg tctggcccag gaaattaaca tttcagcttc cgatattgta gatcttctga
    16441 ccgctaaaag tcttgcgcgc gacacgggtt tgtttatggc catgcagcgc ggacatatga
    16501 acgttattaa tactattttt aacgcattac ccactctttt taatacgttt aaattcgata
    16561 aaaaaatatg aagcccctcc tcctggcaaa taattctaat gaatatcccg gtttgttttc
    16621 agcgatacag cataaacaac aaaacgttgt agagacggtt tatcttgctt tatctgacca
    16681 tgcacgcctg tttggattta ccgctgaaga tattatggat ttttggcaac acaaagcgcc
    16741 acaaaaatac tctgcctttg agttggcttt tgaatttggt caccgggtta ttgctgaatt
    16801 aatccttaat acattaaata agatggctga aagctttggc tttacggata accctcgata
    16861 cattgcggag aaaaattata tggaagcttt actcaaaaaa gcatctcccc ataccgtacg
    16921 ctaaatcttt gcctgcgccg atacgcataa acatcttcca catgcccttc acgtatgcgg
    16981 ttttgtagtg cgcgccagta atcagcgcgg aacaggtcgg cgtgcatctc ttcaaacagc
    17041 ggaccaatac gcgggtcggc gcatagccag tggcgaaact cttccgggaa aacatcgccc
    17101 ggcgagacgc tgtaccacgg ttcgctggca agttcgtctt ccggatagcg cggcggcggg
    17161 atgtcgcgga aattcacttc cgtcatgtag caaatttcat cgtaatcata aaaaaccaca
    17221 cgcccgtgac gggtgacacc gaagttttta aacagcatgt cgccagggaa aatgttagca
    17281 gcggcaagct ggcgaatagc gttaccgtat tcttcaatgg cgtcgcgcaa ctgctgacct
    17341 tccacttgtt ccagccagat attgagcggc accatccgcc gctcgattat agagatggcg
    17401 aattacaatc tgctcaccaa gatcggtgat tttttccgct gcttcctgga gcagtaattc
    17461 cattaatacc ggggaaatat gccgcttctc cagcacaaag ttttcaaact cctgggtgtc
    17521 cgccattcgg cccacacggt cgtgctcttt caccagttga tagcaggcac gaacgtgagc
    17581 ggcagacatc tctttctgcg gcgcgaactt gtctttgatg actttgaata cccgatcaaa
    17641 gcccggcagc gtaaacacca acatcaccat tccacgaata cccggcgctt caatgaactg
    17701 ctcattacag ccctgtagat aaacgagata ttcgcggtag ctttcggttt tggcgtgctt
    17761 ctggcagccg atagccatat acaattcagc agtggtttta cctggcagaa tttcccgtag
    17821 ccactcgacc agcgctgcgg gcagcggtgc ataaaccata aaataagaac gcgcaaagcc
    17881 aaaaacaatg ctcgcttcgg cggtcgtcgt caggcaggta tcaataaata actcgccgtc
    17941 gtccgtctgg tggatcggca gtaagaaggg caacgtgccg gaaggcgtga tcagtttgcc
    18001 caccagccag gcggctttat tgcggtaaaa cagttcgttc gccacctgta aatgactttc
    18061 cgcgaggttg tctgtcccca gcgtttccgt cagatggcga atgatgtaat gaatgtcacg
    18121 gcttttattc tgccagcgca ggcgcagcgg caggtcgctg ataacgcgca tcagtagaga
    18181 ttcccagccg tgatcggggt gaaagtcttt cgccagcggg cggggaatgg tacgaaagcg
    18241 gcgctctggc tgagagctaa agataaaaag ccgctcggga gtaagcgagc ggtggtcaaa
    18301 taaccgacag tacacggagt taaaaaagct ctccgcaatc tcgaagcgcg ggtaatccgg
    18361 caacagccgc gtgtaatgct ctttaacgcg cagtaaaaat gccgcgtccg tactttggcc
    18421 gttagtaatg cagcgcagtt gctccacgac cagaccaacg tgatgatcgt aaagatggat
    18481 acggtttttc atcgcctgct ggacagcatg ccagtcggcc tgttcgaaac gctgctgcgc
    18541 cccggaagtc acttcgagga atcgaccata ctgagcgtcg aagccttgca aaatggtttg
    18601 agcaatcagt aattccaggc cacgcggcat ttagcgccct catcaggagc agagaattgc
    18661 cggatgcggc gtaaacgcct tatccagcct acgttcggca acggttgtag gcatgataag
    18721 acgcgcaagc gtcgcatcag gcatcgtgca ccgattgccg gatgcggcgt aaatgcctta
    18781 tccggcctac agtcagcaac gtttgtaggt tgcttagaac tgcgattctt cagtggagcc
    18841 agtcagcgcg gtgactgaag acgtgccgcc ctgaataata gtcgtcactt tatcgaagta
    18901 acctgtaccc acttcctgct ggtgagatac gaaggtatag ccatctttcg cggcggcaaa
    18961 ttccggctgc tgcactttct caacatagtg cttcatacct tcgccctgcg cataagcgtt
    19021 tgccaggtcg aacatgttaa accacatgct gtggatacct gccagggtga tgaactggaa
    19081 cttgtagccc atatccgaca gctgctgctg gaagctggca atagttttgt cgtcgaggtt
    19141 tttctgccag ttgaacgacg gcgagcagtt ataagccagc agtttgcccg gatatttcgc
    19201 gtggatagct tgtgcaaagc gacgcgccag ttccagatcc ggcgtggagg tttcacacca
    19261 gactaggtca gcatatggcg catacgccag gccacggctg atcgcttgct caatgcccgc
    19321 atgagtacgg aagaagcctt cactggtacg ctcgccggta ataaattcgc tgtcatacgg
    19381 gtcgcaatct gaggtgatca gatccgccgc atcagcatcg gtacgtgcaa ccagcagggt
    19441 tggaacgccc gtcacgtcag ctgccagacg cgccgcgacc agtttctgaa tagcttcctg
    19501 agttggcact aaaactttgc cgcccatgtg accgcatttc ttcactgacg ccagctgatc
    19561 ttcgaagtga actgccgcag caccggcttc aatcatcgct ttcatcagtt caaaggcatt
    19621 caggacaccg ccaaaaccgg cttccgcatc ggcaacgatc ggcaggaagt aatcgacata
    19681 gcgcggatcg cccggctcaa tgcccacgga ccattggatc tgatcggcac gacggaaggt
    19741 gttgttgatc cgctccacca cagctggcac cgagtttgcc ggatagagcg actgatccgg
    19801 atacatgctg gccgccaggt tagcgtccgc cgctacctgc catcccgaca gatagactgc
    19861 ttcaataccc gctttcgcct gttgcagcgc ctgaccgcca gtcagtgcac cgaggctgtt
    19921 gatgtagcct tttttcgact caccgtgcag cagacgccac attttcgctg cgcccagttg
    19981 cgccagcgtg cattcaggat tgactgaacc gcgtaatttc accacatctt ccgcactgta
    20041 tgggcgagta atgccttccc aacgcggttg agtccactct ttctgtaatt cttcaatttg
    20101 ttgtgtacgg gttttcatgt gcagatgctc catagttatg tggtggttta cgctaacagg
    20161 cggtagcctg gcagggtcag gaaatcaatt aactcatcgg aagtggtgat ctgttccatc
    20221 aagcgtgcgg catcgtcaaa acggcccagg gagaaacgtt cttcgcccag ttcgctggca
    20281 atgactttca tctcttcgcc cagcatctgg cggaacaagg ctttggtcac cggtttgcca
    20341 ttgctcaacg ttttttgatg atggatccac tgccagatcg aggtacggga aatttcagcc
    20401 gtcgccgcat cttccatcag gccataaatc ggcacgcagc cgttgccgga gatccacgct
    20461 tcgatgtact gcacagccac gcgaatgtta gcgcgcatac cttcttcggt gcgttcacca
    20521 tcacaaggtg ccagcagctg atcggcagta atcggcgcgt cttgttcgcg caatacttca
    20581 agctgatttt tacgggagcc gagaatgtcg ttgaataccg ccattgccgt gtccgcaagg
    20641 cctgggtgag cgatccatgt gccatcgtga ccgttattgg cttccagcgc tttatccgct
    20701 tttactttgt tgagcacctg gttattacgc tcttcatctt tgctcggaat aaacgcggcc
    20761 atgccgccca tcgcaaaagc accgcgttta tggcaggttt taatcagcag gcgtgagtaa
    20821 gcattcagga atggtttatc catcgtcacg gcctgtctgt ctggcaggac acgatcggga
    20881 tagtttttca acgttttgat atagctgaag atgtaatccc agcgaccgca gttcagacca
    20941 acaatatggt cgcgcagcgc gtgaaggatt tcgtccatct ggaacacggc gggcagcgtt
    21001 tcaatcagca acgtcgcctt gatggtgccg cgcggcagat taaagcgatc ttctgcatag
    21061 ctgaagactt cgctccacca ggccgcttcc tgctaggact gggttttcgg cagatagaaa
    21121 tagggaccgc tgccctttgc caacagtgcc tgatagttgt ggaagaaata gagcgcaaaa
    21181 tcaaacaggc taccggggat tgcctcacca cgccaggtga catgtttttc cggcaagtgc
    21241 agaccgcgta cccgacaaat caaaaccgct ggattgggct tgagctgata aattttgcct
    21301 gcttcattgg tatagctgat ggtaccgtta accgcatcgc gcaggttaat ttgcccgtcg
    21361 atcactttgt tccagtctgg tgccagtgaa tcttcgaaat cggccataaa gactttcaca
    21421 ttggcgttca gtgcgttgat caccatcttg cgctctaccg ggccagttat ctctacgcgg
    21481 cggtcttcta agtccgcagg aatcccgcga attttccaat cagcatcgcg aatggaagcc
    21541 gtttccgaaa taaaatcagg caacgttccg ttatcaatat cttgctgctg ctgaatgcgc
    21601 gctgccagaa gtttattgcg ttgtggcgta aaatgcgtca ccagctcagt cagaaattct
    21661 accgcttcgg cagtaagaat ttgcttctcc tgctcgccat acggccttgt gaaagccagt
    21721 tcatcggttg ttgttgcctg ttcagtcatc gtgcagctcc tcgtcatgga tccgaaaact
    21781 tcccctctgt gaacgaagga acgttgtgcg cttttcgttc agcacaacta agactactca
    21841 tttaaaatgc aaaatcaaaa acaatttcca ttttaattaa aaatacttga taacttattg
    21901 ataacaatat gaataaaatt tattcacttg ctgaggtgcg tttcggaaat aaaaaaggca
    21961 cccgaaggtg cctgaggtaa ggtgctgaat cgcttaacga tcgactatca cagaagatta
    22021 atccagcgtt ggattcatgt gccgtagatc gtatggcgtg atctgataga cgtaatagtt
    22081 gagccagttg gtaaacagca aattaccgtg actacgccag ctcgctcgcg gtttgttttg
    22141 cggatcgttg tgcgggaaat agttatacgg tacatccggg tctagtccgg cttccacatc
    22201 gcgtaaatat tcctgcgcca gcgtttgcgc atcatattcg ggatggcccg tcacaaaggc
    22261 aatgcgctta tctttgctgg caaacagata tgcatcccct tcttccgtct ctgccagaat
    22321 ttccagatcg gtgtaatcac gaatcaacgc tgccggaaag tcagcatagc gcgaatgcgg
    22381 tgccaggaat gaatcatcaa agccacgcgt cagaagcgca tgaggatgga gaatatgatg
    22441 ctcgtaaacg ccagagagtt tgtcggtgcg agtttgctta ggaatgccgt agaggatatt
    22501 gagcgcggcc tgtaccgccc agcagacaaa cagcgtcgag gtgacgtgat ctttcgacca
    22561 ctccagcacc tgtttgatct gcggccagta agcgacatca ttaaactcca ccaggcccag
    22621 cggcgcaccg gttacaatca gaccgtcaaa gttctgttcc tgaatatctt caaagttaca
    22681 gtagaagttg ttcagatgct ctgcgggcgt gttgcgcgat tcacgggaat cgatgcgcaa
    22741 cagctgaata tcgacctgca aaggtgagtt tgaaagcagg cgcagaaact gattttcagt
    22801 ttcaatcttc ttcggcatca ggttaaggat cagaacctta agtggacgaa tttcctgacc
    22861 agacgcacga gaagttgtca tcacaaagac gttttcttca cgcaagaaat tgacggcggg
    22921 tagctcgtcc ggcacacgaa tcggcataac ctgattacct cactacatac gcttatacgt
    22981 ttagacatcc agatagctga agataaccag aaaatttgcc aatgtcgagc ctgcatgttg
    23041 aaggtgagaa agtttcaagg aaaaggctac gttagaatat aagaatgacg aaaaggagag
    23101 aaaatggtta ttagtattcg ccgctcacgg catgaggagg gggaggaact cgttgcgatt
    23161 tggtgtcgat ctgtcgatgc cacccacgat tttttatcag cagagtatcg ggccgagctg
    23221 gaagagctgg tgcgttcctt tctgccggaa gcaccgttgt gggtcgcggt taatgaacgg
    23281 gagcagccgg ttgggtttat gttgttaagt gggcagcata tggatgcgct gtttatcgat
    23341 cctgatgtgt gcggctgcgg cgtgggtcgg atgctggtgg agcatgcgct ctcgatggca
    23401 ccggaactga caaccaacgt taatgagcaa aatgagcagg cggttgggtt ctataagaag
    23461 gtgggtttta aggttacggg acgctctgag gtggacgatt tggggaaacc gtatccgttg
    23521 ctgaatctgg cgtatgtggg ggaataagcc gcccctgccg tgcctgccca agctggagaa
    23581 gacg
//
LOCUS       DAJVPP010000061        23564 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVPP010000061 DAJVPP010000000
VERSION     DAJVPP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.061"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            194..1054
                     /gene="tesB"
                     /locus_tag="OQM34_002448"
     CDS             194..1054
                     /gene="tesB"
                     /locus_tag="OQM34_002448"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1452853.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1103..2389)
                     /gene="amtB"
                     /locus_tag="OQM34_002449"
     CDS             complement(1103..2389)
                     /gene="amtB"
                     /locus_tag="OQM34_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1452854.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MIIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(2419..2757)
                     /gene="glnK"
                     /locus_tag="OQM34_002450"
     CDS             complement(2419..2757)
                     /gene="glnK"
                     /locus_tag="OQM34_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1452855.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(2938..4719)
                     /locus_tag="OQM34_002451"
     CDS             complement(2938..4719)
                     /locus_tag="OQM34_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1452856.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(4712..6484)
                     /locus_tag="OQM34_002452"
     CDS             complement(4712..6484)
                     /locus_tag="OQM34_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1452857.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(6514..6972)
                     /gene="decR"
                     /locus_tag="OQM34_002453"
     CDS             complement(6514..6972)
                     /gene="decR"
                     /locus_tag="OQM34_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS1452858.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7126..7944)
                     /gene="cof"
                     /locus_tag="OQM34_002454"
     CDS             complement(7126..7944)
                     /gene="cof"
                     /locus_tag="OQM34_002454"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS1452859.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8043..9735
                     /locus_tag="OQM34_002455"
                     /pseudo
     CDS             8043..9735
                     /locus_tag="OQM34_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            9800..10495
                     /gene="queC"
                     /locus_tag="OQM34_002456"
     CDS             9800..10495
                     /gene="queC"
                     /locus_tag="OQM34_002456"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1452860.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(10547..10945)
                     /gene="fadM"
                     /locus_tag="OQM34_002457"
     CDS             complement(10547..10945)
                     /gene="fadM"
                     /locus_tag="OQM34_002457"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1452861.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11039..11410)
                     /locus_tag="OQM34_002458"
     CDS             complement(11039..11410)
                     /locus_tag="OQM34_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1452862.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(11561..13432)
                     /gene="ppiD"
                     /locus_tag="OQM34_002459"
     CDS             complement(11561..13432)
                     /gene="ppiD"
                     /locus_tag="OQM34_002459"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1452863.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(13624..13896)
                     /gene="hupB"
                     /locus_tag="OQM34_002460"
     CDS             complement(13624..13896)
                     /gene="hupB"
                     /locus_tag="OQM34_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1452864.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14105..16459)
                     /gene="lon"
                     /locus_tag="OQM34_002461"
     CDS             complement(14105..16459)
                     /gene="lon"
                     /locus_tag="OQM34_002461"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1452865.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(16647..17921)
                     /gene="clpX"
                     /locus_tag="OQM34_002462"
     CDS             complement(16647..17921)
                     /gene="clpX"
                     /locus_tag="OQM34_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1452866.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18047..18670)
                     /gene="clpP"
                     /locus_tag="OQM34_002463"
     CDS             complement(18047..18670)
                     /gene="clpP"
                     /locus_tag="OQM34_002463"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1452867.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(18917..20215)
                     /gene="tig"
                     /locus_tag="OQM34_002464"
     CDS             complement(18917..20215)
                     /gene="tig"
                     /locus_tag="OQM34_002464"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1452868.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(20559..20876)
                     /gene="bolA"
                     /locus_tag="OQM34_002465"
     CDS             complement(20559..20876)
                     /gene="bolA"
                     /locus_tag="OQM34_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1452869.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21181..21759
                     /locus_tag="OQM34_002466"
     CDS             21181..21759
                     /locus_tag="OQM34_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1452870.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            21803..23278
                     /gene="ampG"
                     /locus_tag="OQM34_002467"
     CDS             21803..23278
                     /gene="ampG"
                     /locus_tag="OQM34_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1452871.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5530 a         6507 c         5651 g         5876 t
ORIGIN      
        1 ccagcgggct aactttcctc gccggaagag tggttaacaa aatagtaacg tcaacaagtg
       61 tggcacacat cacgcatttc tgcctgtaat tagcccgtaa ttcagactat ttcacccatc
      121 ggaccagtgg caaatttgcg ttatactcaa ctcactttgg cttgctgcgg cagctttgtt
      181 actggagagt tatatgagtc aggcgctaaa aaatttactg acattgttaa atctggaaaa
      241 aattgaggaa ggactctttc gcggccagag tgaagattta ggtttacgcc aggtgtttgg
      301 cggccaggtc gtgggtcagg ccttgtatgc tgcaaaagag accgtcccgg aagagcggct
      361 ggtacattcg tttcacagct actttcttcg cccaggcgat agtaagaaac cgattattta
      421 tgatgtcgaa acgctgcgtg acggtaacag cttcagcgcc cgccgggttg ctgctattca
      481 aaacggcaaa ccgatttttt atatgacggc ctctttccag gcaccagaag cgggtttcga
      541 acatcaaaaa acaatgccgt ccgcgccagc gcctgatggc ctgccttcgg aaacgcaaat
      601 cgcccaatcg ctggcgcacc tgctgccgcc agtgcttaaa gataaattca tctgcgatcg
      661 tccgctggaa gtccgtccgg tggagtttca taacccactg aaaggtcacg tcgcagaacc
      721 acatcgtcag gtgtggatcc gcgcaaatgg tagcgtgccg gatgacctgc gcgttcatca
      781 gtatctgctc ggttacgctt ctgatcttaa cttcctgccg gtagctctac agccgcactg
      841 catcggtttt ctcgaaccgg ggattcagat tgccaccatt gaccattcca tgtggttcca
      901 tcgcccgttt aatttgaatg aatggctgct gtatagcgtg gagagcacct cggcgtccag
      961 cgcacgcggc tttgtgcgcg gtgagtttta tacccaagac ggcgtgctgg ttgcctcgac
     1021 cgttcaggaa ggggtaatgc gtaatcacaa ttaaaaaaat agccggaggt gaaaaccgtc
     1081 cggctatttt ttgcagtgct tgttacgcgt tataggcatt ctcgccgtgg ctgttgacgt
     1141 ccagcccttc tcgctcttgt tcttccggca cacgtaggcc aaccgtcaga tccgccagtt
     1201 tgtagccaat aaatgccacc acaccggacc agacgatcgt aatggcgatg ctttccaact
     1261 gtaccagcaa ctgatggccc atcgtcacac cttcagcgaa gcccacgccg cccagagaac
     1321 tggcggcaaa aatcccggtc atgatacagc cgacaatgcc acaaacgccg tgcacaccga
     1381 agacatcgca gggatcatcc acccgcagca agcgtttgag catggtaacg ccccacaaac
     1441 ccgccagacc agctaccacg ccgataatca acgcgccgcc aaccccaatg tagccgcacg
     1501 ctggcgtcac gccgaccaga ccggcaatcg cgccagaaca cgctcccagc agagaaggct
     1561 taccacgcag cgcccattca ccgaagatcc agccaagaat tgccgccgcc gttgcgacca
     1621 cagtattcac aaatgccagt gccgcgattt cattcgccgt gcccactgac ccggcgttaa
     1681 agccaaacca accgatatag agaatggcag tcccggtgaa gaccatcggc aggttgtgcg
     1741 gtttaaacgc ctctttaccg aagcctacgc gtttgcctat cagatacgcg cccaccagac
     1801 cggcgattgc tgcgttaatg tgcaccaccg tgccacccgc gaaatccagc gcaccgtgag
     1861 aagccagcaa accaccgccc cacaccatat gcgcaatcgg aatgtaagag agcgtcagcc
     1921 ataccaccac gaaaatcaac acagctgaga agcggattcg ttccgccagc gccccaacta
     1981 tcaagcctac ggtaatgcag gcaaacgatc cctgaaacgc tacgtggata tactgataaa
     2041 tgctgcccat caccgccgtc agttcgatgt tttttagcat caaccagtta atgttgccga
     2101 agaagttgtt gccctcacca aacgccagcg aataaccgta aaccacccag agaatacaga
     2161 ccagtgcaaa cgtcaccgtc acctgcgtca gcatcgacag cacgtttttg ccgcgaatca
     2221 acccaccgta aaacagggca atccccggaa taatcataaa cagcaccagc gcagtacaaa
     2281 tcatcataaa cgcattgtcg gctttatcgg ccaccgcagg tgcagccatt accagtcccg
     2341 gcagcatcgc cagtgaagca agcccagttt ttatcgtcgc tatcttcatt tttcgttcct
     2401 gttgctgtgt gccagagatt acagcgccgc ttcgtcggct tcgccggtac gaatacgaat
     2461 gacgcgttgc aattcagcga cgaagatttt gccgtcgcca atttttccgg tgtaagccgc
     2521 cttactgacg atatcgatca cttcatcgag ttggtcatca gcaatcgcca catcaatttt
     2581 tacttttggc aggaagttga cgctgtattc cgccccccgg tacagctcgg catgcccttt
     2641 ctgacgcccg aaacctttca cttcggtgac ggtcaggccc tgaataccaa tggaagataa
     2701 cgcttcacga acgtcttcca gcttgaatgg tttgattatc acggtcacca gcttcataga
     2761 tcccctccgg tcagaattcg gtaatggttc tgctacacga agaaggtatt gcaagcggtg
     2821 tgccagaaat gaaaaacgag aaagagagca ggaagttaag aagattgaaa atgaaaacgc
     2881 actatgacag tgcacagtgc gttacatttt tgcaccaacg atgaaatatt gcgctattca
     2941 ggcgctcaat gattcctctt cacgcacgct ggctgccagc tcttcgcccg caagttgcag
     3001 ttgatacatc tgccagtagc gtccctgggc cgccagcagt tgctggtgag tgccctgctc
     3061 cacggcttgc ccacgatgaa gcaccagaat ggtgtcggca tcaataatag ttgataagcg
     3121 gtgagcaatc accaccagcg tggtatgttc acgcaccgcc gccagagcat gttgaatcgc
     3181 ctgttcagta ccggagtcaa tgctggcggt tgcctcatca aggatcagga tttgcggcgt
     3241 ctccaccagc acgcgcgcca gtgccagcag ttgcttttgc ccgactgaga gattatttcc
     3301 ctgctcgccc agcggcgtat aaatacaatc actcatgctg cgcgccagct ccgccagttg
     3361 cacggtttcc agcgcctgcc agacgcgttc ttcggagata tcccgcccca gcgtcacatt
     3421 agcgaggaag gtatccgcca gcaccaccgg atcttgctgc accattgcca cgccctggcg
     3481 cagcgcgcta tgacttagcg aacttaatgg acgaccatca aggtgaatct caccttccgt
     3541 tagcgggtaa tagcccatca ataaactggc gagggtactt ttaccactgc cggtatgccc
     3601 gaccagcgcc acaaagttac gcgaaggcac agagagatta atgttcttta gcaccagatt
     3661 gtcatcgcga taagcaaatg acacgttatc gacttcgatg gtgccactct gtaacgggta
     3721 atcatcattg ccatattgct ggcgcggtcc gtccatcagt tcaaacacgc gctcaccagc
     3781 aacaacagcc tgttgcagca tcgcctgttg cgtggtcagt tcgattaagg gttcgttaag
     3841 tcgcccaaga tagctgataa acgcatacaa cacgcccact tcaatggtgc cgctggcgga
     3901 gaagccaaac agcatcaata agccacaaag aatgagcgat gaaaacagac tcagcagcgg
     3961 acgcagcaga aaaccgtcga ggcgcagggt ttgcatccta gccatatagt gtgaacgact
     4021 ggcctccccc atacgttcgc caaatcgcgc ctgctgacga aactgctgga taacgctcat
     4081 gccattgatg atttcgttaa agccgtcgtt gatatccgcc agataggcgc gcacacggcg
     4141 gacaatcggc gtgctgtaac gctggtatat caccattacc accagcacca ccgggaaaat
     4201 cattatcgcc accagcgcca ttcgccagtc gaggctgaac atcgccacca gcatcgcgcc
     4261 caccagtgcg gcactgcgca ggacagttgc cactacggta acgtagagat cgcggatcac
     4321 ttcagtgtca ttagtgacgc gggaaatcac ctgcccgacg ggttgggtat caaactcgct
     4381 taatggctgg cgtaacgcag catccatcac gtcggtacgc aactgttgca ctgcgcctac
     4441 tgccgcccga ttaaacagca gcgactgcgc gtaatgtagc ccggcggcaa acagttgtag
     4501 cccaacatac gccgcagcca gccctgcaac cactttcaac ggcaggttat ttttcgctac
     4561 catattgtcg ataaaatagc tgataagcag cggcccactg acttctgccg ccgccgcaac
     4621 ccacatcatc aggaccgcaa tccccagcgg tttacgccac ggcgaaccat acgctaacag
     4681 gcgcttgaga gtcggccaca gttggctaaa actacgcatc gacggcctcc tcgcgaattt
     4741 ccggaacgtc gtcgagcgcc gcctccagtt gttgatagcg atacatatcg cgataccaac
     4801 cgctttgttg cgccagctca tcatgattgc cacgctgggc gatatgtccg tgctgcatca
     4861 caataatttc actggcttcc gtcagtgccg aaaggcgatg ggcactgatg attaccgttc
     4921 ttccctgtcc ccactgacgc aggttatgca ggatctggtg ctctgtgcgt ccgtccaccg
     4981 ccgaaagcgc atcatcaagg atgaggattt ccgcgttgac taataacgct cgagcaatgg
     5041 agatacgctg tttttgccca ccggaaagca tcacgccgcg ctcgcccact tctgtatcgt
     5101 aaccttgcgg tagacgcaaa atatcgtcat gtacgctggc taaccgcgcg acatgctcaa
     5161 tctcttgctg ggtggcattc gggcaaccca gcgcgatgtt attcgccacg gtgtcagaaa
     5221 aaaggaatgg cgtctggctg accaccgcca ggcggctacg ccagctatcg agttgtaact
     5281 tcgtcagagg aatatcatga aagcgaatat ccccctcgct gacgtcgaaa tgacgctgaa
     5341 tgagcgacaa cagggtactt ttgccggaac cagtcggccc gcaaataccc agcatctgac
     5401 cgggtttcag ggcgaaattg acgttttcca gcgcaggatg gtcagtctgc ggatacgtga
     5461 actggtgaat atttacatcc agttcgccac gcccttccgg cacgggttcg ctaccatcgt
     5521 tcaccaccgg cgcttccgcc agcatcgcgc gaatacggct gtatgcagca ctaccacgtt
     5581 ccacaatgtt aaacatccat gccagcgcca gcattggcca aatcatcaga cctaaataca
     5641 tcataaaact ggtgagctgg cccagcgtta aactgccctg caccaccatc caactaccgc
     5701 cgccaatcgc cagcaagttc gccataccaa tcgcgatata gatggtcggg tcgaagcgag
     5761 catcaatacg cgccacccgc atgttttttt tgccggtatc ttcggcatcc gcggcaaata
     5821 acgccgactg gcgatcttcc agaccaaagg ctttgatcat gcggatactg gtgaggcttt
     5881 cctgggtgcg gtcattaaga ctggaaaacg ccgcctgtgc cagcttaaag cgttcatgca
     5941 aggcatcgcc gttgcgctta atcatgatcg ccatcactgg catcggtaac agggcaaata
     6001 aggtcaactg ccagctaatt tgcgtagaca tcataatcaa cacagcgcag cccattacca
     6061 gtgaatccac cagcgtcagc accccttctc cggcggcaaa cacgacgcga tcgacgtcat
     6121 tggtcgcacg agccatgaga tcacccgtgc gatgacgcag gtaaaactcc gggtgctgcc
     6181 ggcttagctg acggtaataa tcttcacgca gttcaacagc cagttggtaa gacgcgccaa
     6241 acagcaatac gcgccagaca taacgcagga gataaaccac aacggcaatc agcaccatgg
     6301 tggcgatcca catcaggctc agcccggtag taaagtgttg ttctgtcacg ccatcgacaa
     6361 caataccaac cacttttggc ggaaccagtt gcagcatcgc gataatgaca agcaaggcga
     6421 cagccccaag atagcgacgc cattcccgac ggaaatacca gcttaattga gcaaataatc
     6481 gcacgcgttg tgtcctgacc tgattctgga tatttattcg atgggtaaag aagtggtgta
     6541 tttaatctgt tccatcgcga agctggaagt gacgtccgac agccccggca cgctgtttac
     6601 cagacgctta taaaactcgt cgtagcgttt catgtcggca acctggacgc gcatcagata
     6661 atcgtattca ccagccatgc gccagacccc cagcacttct ggcatttcgg taaccaccgt
     6721 gacaaagcgg caataccatt cgctgctgtg atgttgcgtt tttatcagca caaaggcggt
     6781 caggccgagg cctatttttt ccggatccag cagggcgact ttgccgataa ggataccgtc
     6841 gtcctccagc cgtttcagac gcttccagca gggggtggtt gtcagattaa cggcttcagc
     6901 cagtgcctgc aaagagaggg tgcaatcctg ctgcagtaag gccagcagct tacggtcaat
     6961 tttatctaac atagcccttc cacggagaat ttttttctct tttaattcta ttttaaaggt
     7021 gaaatcgcga caatttattc tgtgcggttt aaacaccacg gcacaaaatg acaaattcaa
     7081 cgcaaaaaaa aaccgggcaa ttgcccggtg ctggaaggga tctcgttatt cgggggaata
     7141 aggtagatgt ggatagtcca gccagtgcgt caaatagtga gagacagcct gatttcggca
     7201 atgtccaatc accggtaaat gcgggagctc cgcgcgcagt tgcggcatcg cattgcccat
     7261 aataaacccg ctaccgacgc tgcctaacat ttcgcgatcg ttcatcgcat caccaaaggc
     7321 catgcaatcg cgcaacgata aacctaaatg ttgggtccgc accgtcaatg cagcgccttt
     7381 attgcagccc accggcagca cttcgaggca atccgtggcg gaaaaacaca aatgtgcacg
     7441 ctcgcctaat gcttcgtata gctggatctg caagcgtgta agatcgtcgt gatcgccaca
     7501 gaagcagatc ttggtgacgc tgccgagtgg catttttttg acatcgatta tctgataacg
     7561 aaaaccgcta tagacaaata cctgcaacaa cgcagggctc tctttcccgg taaaccaacc
     7621 gtcgtcattg aagatatgca tgctggctcg ggtatcccat tgctgataca gcaccagctc
     7681 cgcgacatcc gcaggtaaat catcacgatg taaaagttca ccttccagag aatgcacgcg
     7741 cgttccgttg ccggtaatca aatatgcatc cagcgatagc gccccgagaa tatgctgcat
     7801 ctccagcgca tgacgccccg tggcaaaagt gagggtaatg tcgcgttcac gcagtcgcgc
     7861 caaagtagag agggttttct cacctaaatg atggtcaggc atcaataaag tgccatccat
     7921 atcaaatgct accagacgag ccatttcttt ctccactctg tgacacggtt aatggtgatt
     7981 gagtatcacc tgatatatac ggaagtaata gtgaatagtt aataatattg ttccgggttt
     8041 atatgcgatt gctcaaccgt cttaaccagt atcaacgtct gtggcaacct tcagccgcaa
     8101 accgtcaccg tcagcgaact ggccgaacgc tgtttttgca gcgaacgcca tgttcgtacg
     8161 ctgttgcgtc aggcacagga ggcgggatgg ctggagtggc aggcgcagtc aggacgcgga
     8221 aagcgcggac aattacgctt tctggtcacg ccggaatcgc tacgcaatgc gatgatggaa
     8281 caggcactgg aaaccggaaa gcagcaagat gtgctggagc tggcgcaact ggccccaggt
     8341 gagctgcgca ctctgttaca gccatttatg ggcggacaat ggcaaaacga tacacccacg
     8401 ttgcgtattc cctactatcg cccgctcgaa ccgctacaac caggcttttt gcccggccgt
     8461 gccgagcagc atctcgccgg gcagatattt tccggcctga cccgcttcga taataatact
     8521 cagcgcccga ttggcgattt agcgcatcac tgggaaacct ctactgacag gttacgctgg
     8581 gacttttatc ttcgttcaac cctacactgg cataacggcg atgcagtaaa agcctcacac
     8641 ttacaccagc gattattgat gctgttacaa ctgccagcac tggatcaatt atttattagc
     8701 gtgaagcgta ttgaagtcac ccatccgcag tgtctgacct tctttttaca tcgccccgat
     8761 tactggcttg cgcaccggct ggcgagctat tgcagccatc tggcgcatcc gcaattccca
     8821 ctgatcggca ctggtccttt tcgcttaaca caattcacag cggagctggt gcgcctggag
     8881 agccatgatt attaccattt gcgtcatccg ctgcttaaag cggttgagta ctggataact
     8941 ccgccgcttt tcgaaaaaga tttgggaacc agttgtcggc atcccgtgca aatcaccatc
     9001 ggcaaaccgg aggagctgca acgggtcagc caagtcagta gtggcatcag tttaggtttt
     9061 tgctatttaa cgttgcgcaa aagtccccga ctctccctct ggcaggcgcg aaaagtgatc
     9121 tccattattc atcaatccgg tttattacaa acgttagaag tcggagaaaa cctgatcacc
     9181 gccagtcatg cattactgcc aggctggact attccgcact ggcaagtacc ggatgaagtc
     9241 aaactaccga aaaccttgac gctggtttat cacctaccga tagaacttca taccatggca
     9301 gaacgcctac aggcgacact ggcagcggaa ggctgtgaac tcacaattat ttttcataac
     9361 gcaaaaaact gggacgacac gaccctactg gcacacgcag acctcatgat gggcgacaga
     9421 ttaattggcg aagcaccgga atatactctg gagcaatggc tacgttgcga tccactatgg
     9481 ccacatgttt tcgacgctcc agcatatgca catctgcaat cgacgctgga cgcggtgcaa
     9541 gtaatgcctg atgaggaaaa ccgatttaat gccctgaaag cggtttttag ccagttaatg
     9601 gcagacgcga cgctgacgcc gctgttcaac tatcactatc gcattagtgc ccctcccggc
     9661 gtgaacggtg tgcgactgac accgcgcggc tggtttgaat ttaccgaagc ctggcttccc
     9721 gcgccgtcgc aatgaatagc tggtccgggt taatcgcagg cgttaccata acgcttttat
     9781 tgttcaagca ggattatcta tgaaacgtgc tgtcgttgtg ttcagtggag gccaggattc
     9841 caccacctgt ctggtgcagg cattacaaca atatgatgaa gtccattgcg tgacgttcga
     9901 ttacggtcag cgacatcgcg cagaaatcga cgtggcacgc gaactggcgc tgaaactggg
     9961 agcacgcgcg cataaggttc ttgatgtaac gctgctcaac gagctggcgg tcagcagcct
    10021 gacacgtgac agcattccgg tgcctgatta tgaacctgaa gccgatggca tcccgaatac
    10081 gtttgtccca gggcgtaata ttttgttcct gacgctggcg gcaatatatg cgtatcaggt
    10141 aaaagcagaa gccgtaatta ccggcgtctg tgaaacggat ttctccggct acccggattg
    10201 ccgcgatgag tttgtgaaag cactaaacca tgccgtcagt ttgggcatgg cgaaagatat
    10261 tcgttttgaa acgccgctga tgtggattga taaagcggaa acctgggcgc tggcagatta
    10321 ttacggcaaa ctggatttag tccgtaacga aacgttgacc tgctataacg gcattaaagg
    10381 cgacggttgc ggtcattgtg cggcatgtaa tttacgcgcc aacggtttga atcattatct
    10441 ggccgataaa ccgacggtga tggcagcgat gaagcagaaa accgggttga ggtaattatt
    10501 ccgggtgtcg ccggatgcgg cttgagcatc cggcaccaca aaacgtttac ttaaccatct
    10561 gctccagctt ttcgcgcaat tccccttcca gagctaatgc tttctgcgtt ttaagatcaa
    10621 tacaaacaaa cgtaataagc gcatccgcta ccacctgccc ttccggctcc agtgtaatga
    10681 cctggtttaa gatgccgctt ttaccgttta attgctgcaa ctgactggta atagttaaca
    10741 ggtcgcttaa taccgctggg cgacgatagt taatattgat attgaccacg acgaaggcga
    10801 tgttatgggc cgtcatccac tgaaaactgt cgctattttc caccccatcc cagcgggctt
    10861 cttcgagaaa ttcaaggtag cgggcgttgt tgacgtgctg gtaaacgtcg agatgatatc
    10921 cacgaacttt gatttgtgtt tgcatagcgc aataacctta cgttgttgtt ataagtacag
    10981 aggtcataac tggtatgacc tcttcagtct ggcaaaaaat tgccactatg caaattaatt
    11041 acagggttaa taccgccaga ttacgttcca ccagcgaatt gcccatcccc ggcacctgct
    11101 ttagatcctc caccgtttta aacggaccgt actcttcgcg ataactgaca atcgcctgcg
    11161 ctttcttcag gccaacgcca ttcatcgcgc gggctagctc ttccgcgctg gcattattaa
    11221 tgctgacccg ggtgccttct tcgtcactgg ctttcgccgg tactgctgct ttactttgtg
    11281 ctgcaggagc ttccgctttg gtttctaccg ccgtcggttt cgccacagaa gctgccgcca
    11341 gcgcgctatg agacattccg gcacaggcca gggacagggt aatgagcagt gctttaattc
    11401 cgtgtttcat actgttttct ccttgtttgt taacagtgga atcaccttaa cgggagtgaa
    11461 aacagtaaac aaatggcaat gttcagaaat ggaaaaggcc gcgaaagcga ccttttcttg
    11521 ttacagctgc aacgcatttt tttgcgaggc ggcttcagga ttattgctgt tccagcgcat
    11581 cgccaatttt gattttcgcc tctttacgca ggttactcat cagagcttca aagacgattt
    11641 gtgcgttgtt ctgggtgata ccctgcacca tcgctttttt ctgatcttcc ggcattgaac
    11701 cttgtttcac ttcatccagc gccagcagaa ccacattacc ttgcatatcg gtcgccatac
    11761 cgtagctcgg tttgtctttc gctggcagtg gcagtgcaaa cgccgcctgg ctaatcgggt
    11821 cacgaccgga acggcttaag gttttcggct cgccaaattt cagaccggca gcctgcatag
    11881 cttccgcacc tttgccggct ttcaaatcaa ccagcagttt ctcagcatcc actttcgcct
    11941 gttgttcagc tttgttgtgc tgaaccagtg ccttaacttg ttcctgaaca tctgccaacg
    12001 gtttcaccgc ttccggtttg tgctcgctga cgcgcagcac gaatgcgcgg tcgccgtcta
    12061 cggtgatgat gtcagagttg atgcccgacg cgccgttttc acctaccaga ccgccgttaa
    12121 agatagcgtc ggcaaccggc ttgaagttca actcttccgg caggttatct ttgctgaacc
    12181 aacccgtctg agtggcttta acgccggcag cttgctctgc accggccaga gactcggtgt
    12241 cgttgcttgc cgcatcgctc actttctgct gcagcgcata gtacgcatcg agggcttttt
    12301 cgtgtttcac tttcgccgca atgtcgtcac gtacttcgtc taacgatttc actttcgctg
    12361 gctgaatgtc gtccagacgt acaatcagga aaccgaccga agatttgatg acaccagaca
    12421 gttggccttt ttctttcaga ccagcatttt tcagttcgtc ggggatagtg gcatcttcta
    12481 accaacccat atcgccgccg ttacgggcag agataatatc ggcagatttt tctttggcta
    12541 atgcagcaaa atcaccgcct ttattcagct catcaagtac cgctttcgct tcatcttcag
    12601 ttttggtctg gatgatgctg tagcgggtac gctgcggctg ggtgaattga tcctgatgct
    12661 gatcgtagta gctctggata tccgcatcgc taaccggttg ctgcatcgtt gcggcaacca
    12721 gcttgatgta actcacgcgg aattgttccg gcgtcatgaa attgtttttg ttttgttcgt
    12781 agtagctggc aatctcctgt tcggtcacag gctgcttcgc cgccagcgcg ttaacatcga
    12841 tagtcgcctc acgcaccacg cgttgttgcg cgaccagtgc cgccagctcg tcggtttcac
    12901 ctttcagcat aaaatcggta ccggcaacgc cgttaatcag ctgttgggta gtgagctggt
    12961 tacgcagcgc ctgggcgtac tgatcggcgg tcatccccat ctggttgagg ataccgttat
    13021 agcggctgtt atcaaatttg ccgtcaacct ggaaggctgg ggtcgcgaaa atcgcctgtt
    13081 taacctgctc atcactgata cccagtttca gctcacgagc gtactgatcc aacagcgcct
    13141 cgtcgatcag acgattcagc acctgttgac gcagggtttt catatagcct tcgttcgctg
    13201 ccagctcgga gtattgatcg cccagctgtt gctgcatacg attacgctcg ctgttaaagg
    13261 cgttctcgaa ctgcccacgg ctgatttcct ggtcattcac ttttgcggcg tagttattgc
    13321 ctccgccaat caggtaacca ctcacgccgg tcaatatgaa cgacacgata atgataccga
    13381 aaataatctt gagcacgaga ctgtttgcag ccgtgcgtaa gctgtccatc atggtgtaac
    13441 aacactccgc tgtagatgac tgtgtacctc acgcaacgta tcgatgcgcg caaaggccta
    13501 ttgtgacaag aaacgggggc aattgtcagc ccacaagaca cagaaagtca ccgaatacaa
    13561 ataaaaaagg cacatcagta gatgcgccct tgaacttcgt cacatcccca ctggggacaa
    13621 cgcttagttt accgcgtctt tcagtgcttt acctgcacgg aagctcggta ctttagcggc
    13681 agcgatggtg atctctttac cggtctgcgg gttgcggcca gtacgggcag cacgctcttt
    13741 aacggcaaaa gtaccaaaac ctaccagtgc tacatcatcc ccttctttca gagattcagt
    13801 tacggaagca ataatagcat ctaacgcacg gccagccgca gctttagaga tatcagcccc
    13861 tgcagcaatc ttgtcgatca attgagattt attcactctt ctcttcctct ttataattta
    13921 tatcgcactt gaatccttca aggtacgaac gcgcagcagt tatatcaggc cagccatccc
    13981 cttacaacac cccttaataa gggcaagccc gatccgctat ctaacttagc gagacaaaaa
    14041 aaggctggca agcccgaatt agcctgccag ccctgttttt attagtgcat tttgcgcgag
    14101 gtcactattt tgcagtcaca acctgcatgc cagacggttc attttgcagc gccagagtca
    14161 gaacttcctc aatgcgcttc acaggatgaa tgtccagatc ggcaattacg ttgtcaggaa
    14221 tctcttccag atcgcgttta ttttcgaacg gaattagcac tgttttaatc ccgccgcgat
    14281 gcgctgccag gagtttttct ttcaaaccac cgatcggcag tacctgacca cgcagagtga
    14341 tctcaccggt cattgccaca tcggcacgaa ccgggttacc ggtcaggcaa gaaaccagcg
    14401 cggtgcacat agcaataccg gcactcggac catctttcgg cgtcgcacct tccggtacgt
    14461 ggacgtggat gtcacgtttt tcgtaaaaat cagggttgat ccccagtttt tccgcacgcg
    14521 cacgaaccac cgttaacgcc gcctgaatag actcctgcat cacttcgccg agcgaaccgg
    14581 tataggtcag tttgccttta cccggaacac atgcggtttc aatggtcagc aagtcaccgc
    14641 ccacttccgt ccacgccaga ccgattacct gaccgacacg gttttcgtta tcagcgcgac
    14701 catagtcgaa acgctgaaca ccgaggtagt catgcaggtt atcgccgtta atttcgatat
    14761 gttttaatga cttatcgagc agtaactgct taaccgcttt gcggcacagt ttggagattt
    14821 cacgctccag accacgcacg cccgcctcac gggtgtagta acgaataatg ccgataatgg
    14881 cgctatcgtc gacggtcagc tcaccttttt tcagtgcatt acgttcaatc tgcttcggca
    14941 gcaggtgacg tttggcgatg ttcagttttt catcttcggt ataaccggag aggcgaatca
    15001 cttccatacg atccagcagc ggtgccggaa tgttcatgga gttcgacgtc gcgacaaaca
    15061 tcacgtcgct gagatcgtaa tccacttcca ggtagtggtc gctgaacgct acgttctgct
    15121 ctggatccag cacttcaagc agtgcagagg ccggatcgcc tcgcatgtca gaagacattt
    15181 tgtcgatctc atcgagcagg aacagcggat ttttcacgcc cactttcgcc attttctgga
    15241 tcagtttacc cggcatagaa ccgatgtaag tacggcggtg accacggatt tccgcttcat
    15301 cacgcacgcc gcccagcgcc atacggacat atttacgccc ggtggctttg gcaatggact
    15361 gaccaagaga ggttttacct acccccggcg gccctaccag gcagaggatc ggtcccttga
    15421 ttttgttgac acggctttga accgcaaggt actcaaggat gcgatctttc acgcgctcca
    15481 gaccataatg gtcggtatca aggatttcct gcgcctgacg caggtctttt ttgaccttgc
    15541 tgcgcgcatt ccacggtacc tgtaccatcc agtcgatata accacgcact acggtcgctt
    15601 ctgccgacat cggagacatc attttcagct tctgcaactc tgcttccgct ttctcttttg
    15661 cctctttcgg catcttcgcc gcgtcgattt tgcgcttcag ggcttcgttt tcgtccggca
    15721 cgtcgtccat ttcaccgagt tctttctgaa tagctttcat ttgctcgttc agatagtact
    15781 cacgctggga tttctccatc tgctttttaa cgcggttgcg aatgcgtttc tcaacctgca
    15841 gcagatcgat ttccgattcc atcattgcca tcagatattc cagacgttcg ttaacgtcgg
    15901 acatctccag aacggactgt ttgtcagcca gtttcagcgg catatgtgca gcaatggtat
    15961 ccgccagacg cgccggatcg tcgatgctat tcagcgacgt cagcacttct ggtgggattt
    16021 ttttgttcag cttgatgtag ccttcgaact ggctgattgc agtacgcacc agcacttcct
    16081 gttcccgctc atcaatggtc ggcgactcca gatactccgc cttcgcagaa aagtgttcgc
    16141 cattgtcaga gagcgcagaa atacgcgcgc gctgtaaccc ctcgaccagc actttgacgg
    16201 tgccgtcagg cagtttcagc atctgcaata tagaggccac ggtcccgacg gtgaaaagat
    16261 cgtttacacc cggctcatcc gttgaagctt ctttctgcgc gaccagcata atttttttat
    16321 catggtccat cgccgcttcc agacaacgga tagatttttc ccgcccgaca aataagggga
    16381 tgaccatgtg cggataaacc accacatcgc gcagcggcaa tacggggatt tcaatgcgtt
    16441 cagaacgctc aggattcata gagctctctc ttagtttaat ttccgccagg taatcagatg
    16501 acacgactgt gcttcacgcc atctattaac atgtacgtca gtatatgggg atgtttcccc
    16561 cacattcaac gccgagaata gaggaaaaat taaaggggag ataaaatccc ccctttttgg
    16621 ttaactaatt gtatgagaat ggttaattat tcaccagatg cctgttgcgc ttccggctta
    16681 ccataaatca gcaacggttt gctttgacca tcaattaccg actcgtcgat aaccactttt
    16741 tcgacgtctt ccatggacgg cagatcgtac atggtatcga gcagtgcggc ttctacgatg
    16801 gaacgcaggc cacgggcacc ggttttacgc gccatcgctt tcttagcgat agcatccagc
    16861 gcctcgtcac ggaattccag atccacgcct tccagattaa acagcgcctg atactgcttg
    16921 gtcagggcgt ttttcggctc tttgaggatc tgaatcagag cttcttcgct cagttcattc
    16981 aacgttgcta caaccggcag acgaccaatg aactcaggga taagaccaaa cttgatcaga
    17041 tcttccggtt caacctgcgc cagcagctcg ccttcgcttg ctttgtcgga cttcgctttt
    17101 accgtcgcgc caaaaccaat gccggagccg gtttctacac ggtgggaaat cactttatcc
    17161 agaccggcaa acgcaccgcc acaaataaac aggatcttag aggtatcaac ctgcaagaat
    17221 tcctgctgcg gatgtttacg cccaccttgc ggtggaacag cagctaccgt accttcgatc
    17281 agtttcaaca gtgcctgctg tactccttca ccggaaacgt ctcgggtaat ggacgggttg
    17341 tctgacttac gagaaatctt gtcgatttca tcgatgtaga caatcccgcg ctgcgctttc
    17401 tgtacgtcgt aatcgcactt ttgcaacagc ttctgaatga tgttttcaac gtcttcgccc
    17461 acataaccgg cttcggtcag cgtggttgcg tcggccatgg tgaacgggac gtccagcagg
    17521 cgcgccagcg tttcagccag cagcgtttta ccggaaccgg tcggaccgat cagcagaatg
    17581 ttacttttgc ccaactcgac accattgctg gtatcgccgt tgcgcagacg tttgtaatgg
    17641 ttgtataccg cgaccgccag cacttttttc gcctgttcct ggccgataac gtaatcgtcc
    17701 aggtggttgc gaatttcatg cggcgtcggt agcgcactgc gttcacgatg cggtgcaact
    17761 tctttaatct cttcgcgaat gatgtcgtta cataaatcaa cacattcgtc gcagatatac
    17821 acggatggac cggcaatcag cttgcgcact tcatgctggc ttttgccgca aaaagagcaa
    17881 tacagcaatt tgcctgagcc atctttgcgt ttatctgtca tgggtcaaaa cctcttcttt
    17941 gttctttgtg ccgcacacga cgacgcaaat gccattctca ggcgcaagcc gcttacagcg
    18001 ttgtgccgcc ctagataagt atagcggcac agttgcgcct ctggcatcaa ttacgatggg
    18061 tcagaatcga atcgaccaga ccgtattcca ccgcttcagg ggcggaaagg aagcgatcgc
    18121 gctcggtatc acgttcaatc tgttctaatg attgacccgt atgaagcgcc ataagttcat
    18181 tcatgcgccc tttaactttc agaatttcac gggcatgaat ttcgatatcg gtcgcctggc
    18241 cctggtagcc gcccaacggt tggtgaatca tcacgcgcga attcggcagg caaaaacgtt
    18301 taccttttgc ccctgcggtc aacaagaaag cgcccatcga ggccgcctgg cccatacaga
    18361 tggtgctaac atcaggcttg ataaactgca tggtgtcata gatagacatc ccggcagtga
    18421 tcaccccgcc tggggagtta atgtacagat agatatcttt ttctgggttt tccgcttcca
    18481 ggaacagcat ctgcgccaca atcaggttag ccatgtggtc ttcaacctgg ccagtcagaa
    18541 aaatgacgcg ttccttaagt agacgagaat agatatcaaa agagcgctca ccgcgtgagg
    18601 tctgttcaat gaccatcggc accagcgcca tatggggtgc aaagttatct cgttcgccgc
    18661 tgtatgacat ttccgtctcc tggataaaat tgaaaaaaac ctgctgtacc gattgtaacc
    18721 ttatggacgg attatcagct agtccctgtg ttatgggtac ggcatcacca tatttcaagc
    18781 ataacaatct tttgttgtta cgctaacacc gaaagaggct tttcgcacgg ctccatgcaa
    18841 aattcatgac aaaaaaaagc ccaccacctg gcggtgacgg gcctttgtgc gaatttagcg
    18901 cgttatgctg cgtaaattac gcctgctggt tcatcagctc gttgaaagtg gtttcttttt
    18961 cagtcacttt cgctttcgcc agtacagctt caacagcctg ttcttccaga gcaacattgc
    19021 gcatgttgtc catcagttct ttgtttttgc tgtagaactc gataacttct ttcggatctt
    19081 cgtacgcaga agccatctct tcgatcaggc ctttcacgcg ctcttcgtca gctttcagct
    19141 cgttggtgcg gataacttcg cccagcagca ggccaacaac tacgcggcgt ttagcctgtt
    19201 cttcgaacag ttcgcgcggc agttccagag cttgtttttc gttgccaccg aaacgctgtg
    19261 cagcctggcg acgcagaacg tcgatttcgc tgtcgatcag cgcagccggt acgtcgatgt
    19321 cgttagcttt taccagacct tcgatcgcct gagacttaac gcggttacgg atggcgctct
    19381 tcagctcgcg ctccatgttt ttacgcactt cagcgcgcag accttctacg gaaccatctt
    19441 caacgccgaa acgtttgatg aattctgcag tcagttccgg cagttcacgc tcttcaactt
    19501 tcttcaggtt gatagcgaat ttcgctgctt tacctttcag gttttctgcg tggtattctt
    19561 ccgggaaggt cacgtcgatg gtgaactctt cgccagcttt gtggcctttg ataccgtctt
    19621 caaagcccgg gatcatacga ccctggccca tcgccagtac gaaatcagac gctttaccgc
    19681 cttcgaactc ttcgccgtct acagaaccgg tgaagtcgat ggttacgcgg tcttctgctt
    19741 caacagcgcc gtctttttct ttccaggtcg cctgctgttt acgcagagta tccagcatgc
    19801 cgtcaacgtc agcgtcggtc acttcaacga tcggtttttc aacttcgatc gcttccagac
    19861 cctgcagttc aacttccgga taaacttcaa actctacaga gtaagtgaag tcttcaccca
    19921 gcttgtattc gcccggaaca taagtcggtg cgccagccgg attgattttt tctttaatga
    19981 tggcgtcaat gaagttacgg ctcatcaggt cacccagaac gtcctggcgt acagacgcgc
    20041 cataacgctg agcaacgata ttcattggca ctttgccttt gcggaagccg tcaatacgta
    20101 cttttttcgc aacgttgacc agctcgcttt taacagcggt ctcgatgctg tcagcagcga
    20161 tagtaatcgt tacacggcgg ccaaggcctt gagtggtttc aactgaaact tgcatcttgt
    20221 tacctcaaaa aatcacagtg ctcggtcaac tcttaccccg caagcacagg ttgttggctt
    20281 cgcggaaccg ggatgttctt ataatcaatc acattccctg ttaccagaat catcccgaaa
    20341 gcattcaaat aggacgcggc attatagcgg catcactttt atgagtcgag aacggttatt
    20401 gcgcgctgtc gcgtattttt tcacaattcc cgggcaattt tcgtctgaat gctgggcaaa
    20461 aagcgcaaaa tccagacaaa acaaaacggc ccgcaggccg tttttcataa aatctttagc
    20521 aacatactgg aaaagcgccg acagttgcaa atgcgttttt acgcgatgct tcctgctcca
    20581 cgacagggag gagaggcaaa gacggtgtcc tgcaaccctt cccactcctt aatagtgtaa
    20641 gtatgcagag ccagcgcatg aacggtagta gagagttcct ccgctaaagt actgtaaatc
    20701 attcgatgac gattcagaaa acgttcaccc gtaaaacgat cgctgaccag cacaacttta
    20761 aaatggcttt cagagccggc tgggacattg tgacgatagc tttcatccac tacttcgagg
    20821 aatacgggtt ggaacgccgc ccttaatttt tcttctatcc gctcacgtat catcatgaaa
    20881 tatctcctcc gacaacgctg agatgtcatc catcccttta aatactagcc gcttttaccg
    20941 tttccattgc agcttaacaa caaatattta ggtttcgtcc tggtttttcc gtcaagcgta
    21001 tgaagtttac tgatttaaag attgaaacac aggcctcgca tcagcgtttc ttttaccagg
    21061 ggtgattatt tcgtcaacat gatgaattta catgccttac ccacttcccc tcgccgttgg
    21121 caatgttatg atggcgggaa tttttaccaa acacgcttaa gagtctctga gagcccgaac
    21181 atgttcaaaa aaatcctctt cccgttagtt gctctgttta tgcttgcagg atgcgcaaaa
    21241 ccgccaacaa ctattgaagt ttccccgacg attacactgc cacagcagga tccaagcctg
    21301 atgggcgtca ccgtaagcat taatggtgcc gatcagcgta ccgatcaggc gctggcaaaa
    21361 gtcacccgcg ataatcaaat cgttaccctg accgcctccc gcgctctgcg tttcctgctg
    21421 caagaagtgc tggaaaaaca gatgaccgcg cgtggttaca tggttggtcc gaatggcccg
    21481 gttaatctgc aaatcatcgt tagccaactg tatgctgacg tgtcccaggg caacgtgcgc
    21541 tacaacatcg ctaccaaagc ggatatcgcc atcatcgcta ccgcgcagaa tggcaacaaa
    21601 atgaccaaaa actatcgtgc cagctacaac gttgaaggtg cgttccaggc ctccaacaaa
    21661 aatatcgccg atgcggttaa cagtgtgctg accgatacca tcgctgatat gtctcaggac
    21721 accagcattc acgaattcat caagcagaac gcgcgttaat ttctgccctc tggcccggtg
    21781 caagccgggc ctgtagacgc ccatgtccag tcaatattta cgtatttttc aacagccgcg
    21841 ttcagccata ttgctgatcc tgggttttgc ttccgggcta ccgctcgccc tgacatccgg
    21901 caccttacag gcctggatga cggtcgaaaa tatcgatctc aaaaccattg gtttcttctc
    21961 tctggtaggc caggcttacg tttttaaatt cctctggtca ccgctgatgg accgctacac
    22021 gcccccattt tttgggcggc ggcgcggttg gctgctcgcc acgcaaatcc tgttattagt
    22081 cgccattgcg gcgatgggtt ttctcgaacc aggcacccaa ctccgctgga tggcggcgct
    22141 ggcggtggtg atcgcttttt gctctgcctc ccaggatatt gtcttcgatg cgtggaaaac
    22201 cgatgtgctc ccggcagaag aacgtggtgc gggcgcggca atcagcgtgc tgggttaccg
    22261 tttagggatg ctggtttccg gcggcctagc cctgtggctg gcagataaat ggttgggctg
    22321 gcagggcatg tactggttga tggcggcgct gttgatcccc tgtattatcg cgacgttgct
    22381 tgcaccagaa ccaaccgaca ccattcctgt gcccaaaacg ctggaacaag cggttgttgc
    22441 acctctgcga gatttctttg gtcgcaataa tgcctggctt attttgcttc ttatcgtgct
    22501 gtataagctg ggcgacgcat tcgccatgag cctgacaacc acgtttttga ttcgcggcgt
    22561 cgggtttgat gcgggtgaag taggcgtggt aaacaaaacg cttggcttac tggcgaccat
    22621 tgttggcgca ttgtacggtg ggattttgat gcagcgcctg tcgctgttcc gggcgctgct
    22681 gattttcggc attttacaag gtgcgtctaa cgctggttac tggctgctgt cgattactga
    22741 taagcatctc tacagcatgg gcgcagccgt ctttttcgaa aacctctgtg gcgggatggg
    22801 cacatcagcc tttgtcgcgc tgttaatgac gctgtgtaat aagtcatttt ccgctactca
    22861 atttgccctg ctctcagcgc tttccgctgt agggcgagtt tatgtcggcc ccgtggcggg
    22921 ttggtttgtt gaagcacacg gctggtcgac attctatcta ttctccgtcg ccgctgccgt
    22981 accggggctt attttgctgc tggtttgccg ccagacgctt gaatatacac gagtaaatga
    23041 caactttatc tcccgtaccg aatatccggc aggttatgcc tttgccatgt ggacactggc
    23101 ggcgggcgtc agcctgttgg ccgtgtggtt acttctgttg acgatggacg cgctggattt
    23161 gacgcacttc tctttcctgc ctgctctgct ggaagtcggg gttttagtcg ccctttctgg
    23221 cgtcgtgctt ggtggtttgc tggattatct ggcgctacga aaaacgcatc tgacgtaatc
    23281 tgtaaatatt atttagctgt agtaatcact cgccgtaatt attacggcta aataaacatc
    23341 agcatcgctt attaatattt gttcttttgt gcggcttagc gtttggaaat tattgacgcc
    23401 atttataaat tctatttttc ttacacgatt cagctaatga gtctttattt tctcaccacc
    23461 cagttgtcac tttaatgata attatttgtt aaataattgt tttatttcgc attggttata
    23521 ccaattgccc gccaagactc cttctgcact ccccttttgt tata
//
LOCUS       DAJVPP010000062        23505 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVPP010000062 DAJVPP010000000
VERSION     DAJVPP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.062"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            431..2359
                     /gene="thrS"
                     /locus_tag="OQM34_002468"
     CDS             431..2359
                     /gene="thrS"
                     /locus_tag="OQM34_002468"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1452872.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2363..2905
                     /gene="infC"
                     /locus_tag="OQM34_002469"
     CDS             2363..2905
                     /gene="infC"
                     /locus_tag="OQM34_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1452873.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3002..3199
                     /gene="rpmI"
                     /locus_tag="OQM34_002470"
     CDS             3002..3199
                     /gene="rpmI"
                     /locus_tag="OQM34_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1452874.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKTTKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3252..3608
                     /gene="rplT"
                     /locus_tag="OQM34_002471"
     CDS             3252..3608
                     /gene="rplT"
                     /locus_tag="OQM34_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1452875.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3725..3849
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3731..3775
                     /gene="pheM"
                     /locus_tag="OQM34_002472"
     CDS             3731..3775
                     /gene="pheM"
                     /locus_tag="OQM34_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1452876.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4058..5041
                     /gene="pheS"
                     /locus_tag="OQM34_002473"
     CDS             4058..5041
                     /gene="pheS"
                     /locus_tag="OQM34_002473"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1452877.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5056..7443
                     /gene="pheT"
                     /locus_tag="OQM34_002474"
     CDS             5056..7443
                     /gene="pheT"
                     /locus_tag="OQM34_002474"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1452878.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7448..7747
                     /gene="ihfA"
                     /locus_tag="OQM34_002475"
     CDS             7448..7747
                     /gene="ihfA"
                     /locus_tag="OQM34_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1452879.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7848..8828
                     /gene="btuC"
                     /locus_tag="OQM34_002476"
     CDS             7848..8828
                     /gene="btuC"
                     /locus_tag="OQM34_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1452880.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8891..9442
                     /gene="btuE"
                     /locus_tag="OQM34_002477"
     CDS             8891..9442
                     /gene="btuE"
                     /locus_tag="OQM34_002477"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1452881.1"
                     /translation="MQDSILTTVVKDIDGEVTTLGKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVALGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9442..10191
                     /gene="btuD"
                     /locus_tag="OQM34_002478"
     CDS             9442..10191
                     /gene="btuD"
                     /locus_tag="OQM34_002478"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1452882.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10269..10733
                     /locus_tag="OQM34_002479"
     CDS             10269..10733
                     /locus_tag="OQM34_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1452883.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            10980..11693
                     /gene="ydiV"
                     /locus_tag="OQM34_002480"
     CDS             10980..11693
                     /gene="ydiV"
                     /locus_tag="OQM34_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS1452884.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11757..13193
                     /locus_tag="OQM34_002481"
     CDS             11757..13193
                     /locus_tag="OQM34_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1452885.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13197..13388)
                     /gene="hemP"
                     /locus_tag="OQM34_002482"
     CDS             complement(13197..13388)
                     /gene="hemP"
                     /locus_tag="OQM34_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1452886.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13520..14566)
                     /gene="aroH"
                     /locus_tag="OQM34_002483"
     CDS             complement(13520..14566)
                     /gene="aroH"
                     /locus_tag="OQM34_002483"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1452887.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14723..15556)
                     /gene="ppsR"
                     /locus_tag="OQM34_002484"
     CDS             complement(14723..15556)
                     /gene="ppsR"
                     /locus_tag="OQM34_002484"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1452888.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            15888..18266
                     /gene="ppsA"
                     /locus_tag="OQM34_002485"
     CDS             15888..18266
                     /gene="ppsA"
                     /locus_tag="OQM34_002485"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1452889.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18323..19969)
                     /gene="fadK"
                     /locus_tag="OQM34_002486"
     CDS             complement(18323..19969)
                     /gene="fadK"
                     /locus_tag="OQM34_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCS1452890.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20026..20319)
                     /locus_tag="OQM34_002487"
     CDS             complement(20026..20319)
                     /locus_tag="OQM34_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1452891.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20316..21605)
                     /locus_tag="OQM34_002488"
     CDS             complement(20316..21605)
                     /locus_tag="OQM34_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1452892.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21661..22599)
                     /locus_tag="OQM34_002489"
                     /pseudo
     CDS             complement(21661..22599)
                     /locus_tag="OQM34_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(22619..23383)
                     /locus_tag="OQM34_002490"
     CDS             complement(22619..23383)
                     /locus_tag="OQM34_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCS1452893.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5774 a         5757 c         6209 g         5765 t
ORIGIN      
        1 ttgtgtcact tttatcattg tgatatgacc acttcgtcat taatctcatg agcgtttaaa
       61 atactaccgc caaatcacgc aacgaacttt gctggattca gtttatgctg ctgtaaatcc
      121 gctcgcagta aacctttcag acgcaccgtg atgttatcag tggttcttta agcgttttgc
      181 tggtgtactc actacaaacg aattgcgaat cgaatcaatg tgaaacggaa aggtacaatc
      241 tccctttttt tagtgttaac atcgttcaac cgggtggaag gtgagaggtt aatgtctttc
      301 atttgtgtac ctgattgcga accaatttag catttgttgg ctagatggtt tcgcaatgaa
      361 ctgttaataa acaaattttt ctttgtatgt gatctttcgt gtgggtcacc actgcaaata
      421 aggatataaa atgcctgtta taactcttcc tgatggcagc caacgccatt acgaccacgc
      481 tgtaagcccc atggatgttg cgctggacat tggtccaggt ctggcgaaag cctgtatcgc
      541 agggcgcgtt aatggcgaac tggttgatgc ttgcgatctg attgaaaacg acgcacaact
      601 gtcgatcatt accgccaaag acgaagaagg tctggagatc attcgtcact cctgtgcgca
      661 cctgttaggg catgcgatta aacaactttg gccgcatacc aaaatggcaa tcggcccggt
      721 tattgacaac ggtttttatt acgacgttga tcttgaccgc acgttaaccc aggaagatgt
      781 cgaagcactc gagaagcgga tgcatgagct tgctgagaaa aactacgacg tcattaagaa
      841 gaaagtcagc tggcacgaag cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt
      901 ttccattctt gacgaaaaca tcgcccatga tgacaagcca ggtctgtact tccatgaaga
      961 atatgtcgat atgtgccgcg gtccgcacgt accgaacatg cgtttctgcc atcatttcaa
     1021 actaatgaaa acggcagggg cttactggcg tggcgacagc aacaacaaaa tgttgcaacg
     1081 tatttacggt acggcgtggg cagacaaaaa agcacttaac gcttacctgc agcgcctgga
     1141 agaagccgcg aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt accatatgca
     1201 ggaagaagcg ccgggtatgg tattctggca caacgacggc tggaccatct tccgtgaact
     1261 ggaagtgttt gttcgttcta aactgaaaga gtaccagtat caggaagtta aaggtccgtt
     1321 catgatggac cgtgtcctgt gggaaaaaac cggtcactgg gacaactaca aagatgcaat
     1381 gttcaccaca tcttctgaga accgtgaata ctgcattaag ccgatgaact gcccgggtca
     1441 cgtacaaatt ttcaaccagg ggctgaagtc ttatcgcgat ctgccgctgc gtatggccga
     1501 gtttggtagc tgccaccgta acgagccgtc aggttcgctg catggcctga tgcgcgtgcg
     1561 tggatttacc caggatgacg cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt
     1621 taacggatgt atccgtttag tctatgatat gtacagcact tttggcttcg agaagatcgt
     1681 cgtcaaactc tccactcgtc ctgaaaaacg tattggcagc gacgaaatgt gggatcgtgc
     1741 tgaggcggac ctggcggttg cgctggaaga aaacaacatc ccgtttgaat atcaactggg
     1801 tgaaggcgct ttctacggtc cgaaaattga atttaccctg tatgactgcc tcgatcgtgc
     1861 atggcagtgc ggtacagtac agctggactt ctccttgccg tctcgtctga gcgcctccta
     1921 tgtgggcgaa gacaacgagc gtaaggtacc ggtaatgatt caccgcgcaa ttctggggtc
     1981 gatggagcgt ttcatcggta tcctgaccga agaattcgct ggtttcttcc cgacctggct
     2041 tgcgccagtt caggttgtta tcatgaatat taccgattca cagtctgaat acgttaacga
     2101 attgacgcaa aaactatcaa atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa
     2161 gattggcttt aaaatccgcg agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg
     2221 tgataaagag gtggaatcag gcaaagttgc cgttcgcacc cgccgtggta aagacctggg
     2281 aagcatggac gtaaatgaag tgatcgagaa gctgcaacaa gagattcgca gccgcagtct
     2341 taaacaattg gaggaataag gtattaaagg cggaaaacga gttcaaacgg cgcgccctaa
     2401 ccgtatcaat ggcgaaattc gcgcccagga agttcgctta acaggtctgg aaggcgagca
     2461 gcttggtatt gtgagtctga gagaagctct ggagaaagca gaagaagccg gagtagactt
     2521 agtcgagatc agccctaacg ccgagccgcc ggtttgtcgt ataatggatt acggcaaatt
     2581 cctctatgaa aagagcaagt cttctaagga acagaagaaa aagcaaaaag ttatccaggt
     2641 taaggaaatt aaattccgtc ctagtacaga tgaaggcgac tatcaggtaa aactccgcag
     2701 cctgattcgc tttctcgaag agggtgataa agccaaaatc acgctgcgtt tccgcggtcg
     2761 tgagatggcg caccagcaaa tcggtatgga agtgcttaat cgcgtgaaag acgatttgca
     2821 agaactggca gtagtcgaat ccttcccaac gaagatcgaa ggccgccaga tgatcatggt
     2881 gctcgctcct aagaagaaac agtaagggct tcaagtagca ctatctgtgg agcctttggg
     2941 cttcacaggc attgttcgcc tttgtttcgt ttattaacaa tgcgaagtgg aagttattaa
     3001 aatgccaaaa attaagaccg tacgcggtgc tgctaagcgc ttcaaaaaaa ccggtaaagg
     3061 tggttttaag cacaagcacg ctaacctgcg tcacattctg actaaaaaaa cgaccaaacg
     3121 taaacgtcac ctgcgtccga aagccatggt ttccaaaggc gatctgggcc tggtaatcgc
     3181 gtgcctgccg tacgcataag ccgttaacgt ttttaacttt ttaattagaa tatagataca
     3241 ggagagcaca tatggctcgc gtaaaacgtg gtgttattgc acgcgcacgt cacaagaaaa
     3301 ttttgaaaca agctaaaggc tactacggtg cgcgttctcg cgtataccgc gttgccttcc
     3361 aggctgttat caaagctggt cagtatgctt accgtgaccg tcgtcaacgt aagcgtcagt
     3421 tccgtcaact gtggattgcg cgtatcaacg cagcagcacg tcagaacggt atttcttaca
     3481 gcaaattcat caatggcctg aaaaaagcct ctgttgaaat cgaccgtaag atcctggctg
     3541 atatcgcagt attcgacaaa gtagcgttca ccactctggt tgaaaaagcg aaagcagctc
     3601 tggcataagc cagttgaaag agggagctag tctccctctt ttcgtttcaa cgccatcaaa
     3661 acattgactt ttatcgccgt agccttttca ataaaggtct tttgaagagt accaaaaggt
     3721 aacgcaagca atgaatgctg ctattttccg cttctttttt tactttagca cctgaatcca
     3781 ggaggctagc gcgtgagaag agaaacggaa aacagcgcct gaaagcctcc cagtggaggc
     3841 tttttttgta tgcgcgtttt gaaaaataaa aagccagtct atcaggcgta attgtcgcag
     3901 ccagtctgga cacggccagt gcggagaaac cggagcgtac ttaagtacgt gaggatttcg
     3961 agcactgccc gggaccaaaa tggcaagtaa aatagcctga tgggataggc tctaagtcca
     4021 acgaaccagt gtcaccactg acacaatgag gaaaaccatg tcacatctcg cagaactggt
     4081 tgccagtgcg aaggcggcca ttagccaggc gtcagatgtt gccgcgttag ataatgtgcg
     4141 cgtcgaatat ttgggtaaaa aagggcactt aacccttcag atgacgaccc tgcgtgagct
     4201 gccgccagaa gagcgtccgg cagctggtgc ggttatcaac gaagcgaaag agcaggttca
     4261 gcaggcgctg aatgcgcgta aagcggaact ggaaagcgct gcactgaatg cgcgtctggc
     4321 ggcggaaacg attgatgtct ctctgccagg tcgtcgcatt gaaaacggcg gtctgcatcc
     4381 ggttacccgt accatcgacc gtatcgaaag tttcttcggt gagcttggct ttaccgtggc
     4441 aaccgggccg gaaatcgaag acgattatca taacttcgat gctctgaaca ttcctggtca
     4501 ccacccggcg cgcgctgacc acgacacttt ctggtttgac gctacccgcc tgctgcgtac
     4561 ccagacctct ggcgtacaga tccgcaccat gaaagcccag cagccaccga ttcgtatcat
     4621 cgcgcctggc cgtgtttatc gtaacgacta cgaccagact cacacgccga tgttccatca
     4681 gatggaaggt ctgattgttg ataccaacat cagctttacc aacctgaaag gcacgctgca
     4741 cgacttcctg cgtaacttct ttgaggaaga tttgcagatt cgcttccgtc cttcctactt
     4801 cccgtttacc gagccttctg cagaagttga cgtcatgggt aaaaacggta aatggctgga
     4861 agtgctgggc tgcgggatgg tgcatccgaa cgtgctgcgt aacgttggca tcgacccgga
     4921 agtttactct ggtttcgcct tcgggatggg gatggagcgt ctgactatgt tgcgttacgg
     4981 cgtcaccgac ctgcgttcat tcttcgaaaa cgatctgcgt ttcctcaaac agtttaaata
     5041 aggcaggaat agattatgaa attcagtgaa ctgtggttac gcgaatgggt gaacccggcg
     5101 attgatagcg atacgctggc gaatcaaatc actatggcgg gcctggaagt tgacggtgta
     5161 gaaccggttg ccggtagctt ccacggcgtg gtcgttggtg aagtggttga gtgtgcgcag
     5221 catccgaacg ctgacaaact gcgtgtgaca aaagtgaatg tcggcggcga tcgcctgctg
     5281 gacatcgtct gcggtgcgcc aaactgccgt cagggcctgc gtgtggcggt agctaccatt
     5341 ggtgctgttc tgccgggtga tttcaaaatt aaagcggcga aactgcgtgg cgaaccgtct
     5401 gaagggatgc tgtgctcctt ttctgagctg ggaatttctg acgatcacag tggcattatc
     5461 gaactgcctg cggatgcgcc gattggcacc gacatccgcg aatacctgaa actcgatgat
     5521 aacaccatcg aaatcagcgt gacaccaaac cgtgccgact gcttaggtat cattggtgtt
     5581 gcgcgtgacg ttgccgtgct gaatcagctg ccgctggttg aaccggaaat cgttccggtt
     5641 ggtgcgacca tcgacgacac gttgccgatt acagtcgaag cgccggaagc ctgcccgcgc
     5701 tatcttggcc gtgtggtaaa aggcattaac gttaaagcgc caactccgct gtggatgaaa
     5761 gaaaaactgc gtcgttgcgg gatccgttct atcgatgcag ttgttgacgt caccaactat
     5821 gtactgctcg aactgggcca gccgatgcac gctttcgata aagatcgcat tgaaggcggc
     5881 attgtggtgc ggatggcaaa agagggcgaa acgctggtgc tgcttgatgg cactgaagcg
     5941 aagctgaatg ctgacactct ggtcatcgcc gaccacaaca aggcgctggc gatgggcggc
     6001 atctttggtg gcgaacactc tggcgtgaat gacgaaacgc aaaacgtctt gctggaatgc
     6061 gctttcttca gcccgctgtc tatcaccggt cgtgctcgtc gtcatggcct gcatactgat
     6121 gcgtctcacc gttatgagcg tggcgttgat ccggtactgc agcacaaagc gatggaacgt
     6181 gcgacccgtc tgctgatcga catctgcggt ggtgaggctg gcccggtaat tgatatcacc
     6241 aacgaagcaa cgctgccgaa gcgtgcaacc atcactctac gtcgtagcaa actggatcgc
     6301 ctgataggcc atcatattgc tgatgagcag gtaactgaca ttctgcgtcg tctcggctgc
     6361 gaagtgaccg aaggcaaaga cgaatggcag gcagttgcgc cgagctggcg tttcgatatg
     6421 gagattgaag aagatctggt cgaagaagtc gcgcgtgttt acggctacaa caacatcccg
     6481 gatgagccgg tacaggcaag cctgattatg ggtactcacc gtgaagctga cctgtcgctc
     6541 aagcgcgtga aaacgctgct caacgacaaa ggctatcagg aagtgatcac ctacagcttc
     6601 gttgatccga aagtgcagca gatgatccat ccaggcgttg aagccttact gctggcaagc
     6661 ccgatctctg ttgaaatgtc agcaatgcgt ctttctctgt ggaccggcct gctggcaacc
     6721 gtggtgtaca accagaaccg tcagcagaac cgtgtgcgca ttttcgaaag cggtctgcgt
     6781 ttcgtaccag atactcaggc accgttgggc attcgtcagg atctgatgtt agccggtgtg
     6841 atttgcggta accgttacga agagcactgg aacctggcaa aagagaccgt tgatttctat
     6901 gatttgaaag gcgaccttga atccgttctc gacctgaccg gtaaactgaa tgaggttgag
     6961 ttccgtgcag aagcgaatcc ggcactgcat ccggggcaat ccgcagcgat ttatctgaaa
     7021 ggtgaacgta ttggttttgt tggagttgtt catcctgaac tggaacgtaa actggatctt
     7081 aacggtcgca ctctggtgtt cgaactggag tggaacaagc tcgcagaccg cgtggtgcct
     7141 caggcgcgcg agatttctcg cttcccggcg aaccgtcgtg acatcgcggt ggtggtcgca
     7201 gaaaacgttc ccgcagcgga tattttatcc gaatgtaaga aagttggcgt aaatcaggta
     7261 gttggcgtaa acttatttga cgtgtaccgc ggtaagggtg ttgcggaggg gtataagagc
     7321 ctcgccataa gcctgatcct gcaagatacc agccgtacac tcgaagaaga ggagattgcc
     7381 gctaccgtcg ccaaatgtgt agaggcatta aaagagcgat tccaggcatc attgagggat
     7441 tgaacctatg gcgcttacaa aagctgaaat gtcagaatat ctgtttgata agcttgggct
     7501 tagcaagcgg gatgccaaag aactggttga actgtttttc gaagagatcc gtcgcgctct
     7561 ggaaaacggc gaacaggtga aactctctgg ttttggtaac ttcgatctgc gtgataagaa
     7621 tcaacgcccg ggacgtaacc cgaaaacggg cgaggatatt cccattacag cacggcgcgt
     7681 ggtgaccttc agacccgggc agaagttaaa aagccgggtc gaaaacgctt cgcccaaaga
     7741 cgagtaatct gatctaacta aaaaggccgc tatgcggcct tttttctttt cactgtcgaa
     7801 gagtcaccgt aaaatcaacg ccatgacact tcagcagaac ggataccatg ctgacacttg
     7861 cccgccaaca acagcgacaa aatattcgct ggttattatg cctgtcagtt ttgatgctgc
     7921 tggcgcttct cttaagcctt tgcgccggtg aacaatggat ttcgccaggt gactggttta
     7981 ctcctcgtgg cgaactgttc gtctggcaaa ttcgcctgcc acgtacgctg gctgtattgc
     8041 tggttggtgc ggcgctggct atatccggcg ctgtaatgca ggcgttgttt gaaaatcctc
     8101 tggcagaacc tggactactt ggcgtctcta acggcgcagg cgtggggctt atcgccgcgg
     8161 tattgcttgg gcaagggcaa ctccccaact gggcgctagg gctgtgtgcg attgctggcg
     8221 cgcttatcat cactttaata ctcttacgtt tcgcccgtcg tcatctttcg accagtcggt
     8281 tattgctggc tggcgttgca ttagggatta tctgcagcgc actaatgacg tgggctatct
     8341 acttttccac ctctgttgat ttacgtcagc tgatgtactg gatgatgggc ggttttggcg
     8401 gcgtagactg gcggcaaagc tggctgatgc tggcattgat ccccgtgttg ttgtggatct
     8461 gttgtcagtc caggccgatg aatatgttag cacttggcga gatctcggcg cggcaactgg
     8521 gtttacccat gtggttctgg cgcaatgtgc tggtggcagc gaccggctgg atggttggcg
     8581 tcagtgtggc gctggcgggt gctatcggct ttattggtct ggtgatccca catattcttc
     8641 ggttgtgtgg tttaaccgat catcgcgtat tacttcccgg ctgcgcgctg gcaggggcga
     8701 gcgcattgct gctggccgat attgtagcgc gcctggcatt agctgccgca gagctgccta
     8761 ttggcgtggt caccgcaacg ttgggtgcgc cggtgtttat ctggttattg ttaaaagcag
     8821 gacgttagcc gcaaaaagac ggtctatgat taaaagctaa gatttttact gaccacacct
     8881 aggagaaacg atgcaagatt ccattctgac gaccgtagtg aaagatatcg acggtgaagt
     8941 gaccacgctg gggaagtacg ccggtaatgt gctgttaatt gtcaatgtcg cctcaaagtg
     9001 tggcttaacg ccgcaatatg agcagttgga gaatattcaa aaagcctggg ccgatcgagg
     9061 ttttgtggcg ctgggattcc cgtgcaacca gtttctggaa caagaaccgg gcagcgatga
     9121 agagattaaa acttactgta ccaccacatg gggggtgacg ttcccgatgt tcagtaagat
     9181 tgaagttaat ggcgaaggac gccatccgct gtatcaaaaa ttgattgccg cagcgccgac
     9241 cgcagtcgcg ccggaagaga gcggattcta tgcccgtatg gtcagcaaag gccgtgcacc
     9301 gctgtacccg gatgatattt tatggaattt tgaaaaattc ctggttggca gggacggaaa
     9361 agtcatccag cgtttttccc cggatatgac gccggaagat cccattgtga tggaaagcat
     9421 taaactggcg ttggcaaaat aatgtctatt gtgatgcagt tacaggatgt tgcggaatct
     9481 acccgcctgg ggccgctttc aggcgaggtt cgggctgggg aaatcctgca cctggtgggg
     9541 ccgaatggcg cgggtaagag taccttactg gcgcaaatgg ccggaatgac cagcggtaag
     9601 ggaagcattc agttcgcggg gcaaccactg gaagcatggt ccgcaacaaa actcgcgctg
     9661 catcgcgcct atctttcaca acagcagacg ccgccgtttg caatgccggt ctggcactac
     9721 ctgacactgc atcagcacga taaaacgcgt accgaactac tgaatgatgt cgcaggggcg
     9781 ctggctcttg atgacaaact cggtcgtagc accaatcaac tttccggcgg tgaatggcaa
     9841 cgcgtacgtc ttgctgcggt ggtgttgcaa atcacaccac aagccaatcc cgcaggccaa
     9901 ttgctgcttc ttgatgagcc gatgaacagt cttgatgttg cgcaacaaag tgcgttagac
     9961 aaaattctga gcgcgctgtg tcagcaagga ctggcgattg tgatgagcag tcacgatctc
    10021 aaccacacgt tgcgtcatgc gcatcgggcg tggttgctaa aaggtggaaa aatgctggcc
    10081 agtggacgca gagaagaggt gctcacgccg ccaaatctgg cgcaggccta tgggatgaat
    10141 tttcgccgtc tggatatcga aggtcacaga atgctgattt cgaccatctg atttcattag
    10201 tatcccgaaa atcgggtaat tacgacgcta aattaatgca agaataaaaa acagaggaac
    10261 cgtacggaat gcgtttctgc cttattttga tcacagcact gtttctggcc gggtgtagcc
    10321 accataaagc accgccgcca aatgccagac tttctgattc gattaccgtt attgccggtt
    10381 tgaacgacca gctacaaagc tggcatggca cgccgtatcg ttatggtggc atgacgcggc
    10441 gcggtgtgga ctgttcggga tttgtggttg tgacgatgcg cgatcgtttc gatttgcatc
    10501 tgccccgtga aaccaaacaa caagcctcta ttggcacgca aattgataaa gacgagttgc
    10561 tgcctggtga cctggtcttt ttcaaaacgg gttccggaca aaatggtttg catgtaggta
    10621 tttacgatac caacaaccaa tttatccacg cctctaccag caagggagtg atgcgttcct
    10681 cacttgataa tgtctattgg cagaaaaatt tctggcaggc gagacgaatc tagtatgtcg
    10741 aaaaattgac ggcgatgata catcgccgtc aatttactcc tgaatacagt agtattttgc
    10801 agacaaaaat tccgtaaatg gattattcgt ttataatcag gataatctaa aaaaaggccc
    10861 cctgaaaaag tggttaacat ggaacaaatg aaagttactc cttctgtaat tgttatgtct
    10921 tatggcctca tcccttttag ccggatactg aaaaacatcc ttcgagaggg acggttacca
    10981 tgaagatttt tttggagaat ctttatcatt ctgattgcta tttcctgccg ataagagata
    11041 accagcaact ccttgtcggc gttgagttaa ttacccattt ctccagtgaa gatggcacgg
    11101 ttcgtatccc gacgagtcgg gttatagcgc aacttaccga ggagcaacat tggcaattat
    11161 tttcagagca gttggaatta ctaaaatcat gccaacactt ttttattcaa cataaactct
    11221 ttgcctggct taatttaacg ccacaagtgg ctacattatt attagacagg gataattacg
    11281 caggcgaact attaaaatat ccgtttattg agctgttgat taatgagaat tacccgcatc
    11341 tcaatgaagg aaaagataat cgcgatcttt tgtccttatc gcaaatgtat ccccttgtgt
    11401 taggcaattt gggagcaggt aatagcacaa tgaaagcggt ttttgatggt cttttcaccc
    11461 gcgtaatgct ggagaaaggt tttattcagc aacaaatcac gcatcgctcc ttcgaaccgt
    11521 ttattcgtgc gatccaggcg caaatttccc cttgttgcaa ttgcatcatc gcggggggca
    11581 ttgatacgcc agaaatattg gcacagatta cccccttcga tttccatgct ctccagggat
    11641 gcctgtggcc tgccgttcct ataaatcaga taacgacgtt ggttcagcga taaccctcct
    11701 gtttgctggt gtttaagacg agagtaaccg tctacactat caaacagata ggatctatga
    11761 ccctgtcttt tattacccgc tggcgcgatg aattgccaga aacctataca gcactttccc
    11821 ctacgccttt aaataatgcc cggctgattt ggcataatac cgaactcgct aacacgctga
    11881 gtattccatc gtcgctgttt aaaaatgccg caggtgtctg gggcggcgaa accctactgc
    11941 ctggtatgtc accactggca caggtttaca gtggtcatca gttcgtcgtc tgggcgggcc
    12001 aactgggtga tgggcgcggc attttacttg gcgaacaact gcttgctgat ggcactacaa
    12061 tggactggca tctgaaaggt gctggcctga cgccttattc gcgaatgggt gatggacggg
    12121 cggttttacg ttcgacgata cgagaaagtc tcgccagtga ggcgatgcat tatctgggca
    12181 ttccgacgac ccgcgcgtta agtatcgtca ccagcgattc gccagtgtat cgggaaacgg
    12241 tggagccagg cgcgatgctg atgcgtgtgg caccaagtca tctgcgcttt ggtcatttcg
    12301 aacattttta ctatcgccgc gagccggaaa aggttcgtca gttggctgac tttgccattc
    12361 gtcattactg gtcacatctt gaagacgatg aggacaaata ccgtctctgg tttaacgatg
    12421 ttgtcgcacg taccgcgtcg ttaattgccc aatggcagac ggtcggcttt gcacatgggg
    12481 tgatgaatac cgacaacatg tcgctgctgg gactgacgct tgattacggg ccgtttggtt
    12541 ttcttgatga ttacgaaccc ggttttattt gtaatcactc ggatcatcaa gggcgttata
    12601 gctttgataa tcaacctgct gtcgcgttgt ggaatttaca gcgtctggcg cagacattgt
    12661 caccatttgt tgccgtagat gccctgaatg aggccctgga cagctatcag caggttttgt
    12721 tgacgcatta tggacaacgg atgcggcaga aactgggctt catgacggag caaaaagagg
    12781 ataacgcgct actgaatgaa ttattcagtc tgatggcgcg agagcgcagc gattataccc
    12841 gcacattccg catgctgagt ctgaccgagc agcacagcgc ggcgtcaccg ctacgtgagg
    12901 agtttattga tcgtgcggca tttgatgact ggtttgcccg ttatcggggg cgtttgcaac
    12961 aagacgaggt tagcgatagt gagcgtcagc aactgatgca aagcgttaac ccagctctgg
    13021 tattgcgcaa ctggttggcg caacgggcga ttgaagcggc agaaaagggt gatatgatgg
    13081 aattgcaccg cctgcatgag gcgttgcgaa atcctttcag cgacagagat gatgactatg
    13141 tcagtcgtcc acctgactgg ggtaaacggc tggaagtcag ctgctcgagt taaaggctac
    13201 ttagttaaca gcagcttgcc agcctgagtt ttacggagca gatattcttg cccgtcatga
    13261 tcgataatca gtttgccatc cggacctaac agtgtctggc tggtaattcg ccggatgggc
    13321 tgcggggaag cggtcttgtg attggcatcc gtttcaggcg tgagttttct gctatccgta
    13381 taacgcataa aaattaataa taattgatat aacaatgatt cctattatca atattatggc
    13441 aggccagagc aagcagtaat gtgttgagaa gacaacaagt tgcgcagaaa tgcccatccc
    13501 atcaggaatg ggcacgcatt cagaagcggg tatctaccgc aaaggcgagt ttttcgacca
    13561 ggcgttcggt atcttcccag cccagacacg ggtcggtaat ggattgaccg taagtgagcg
    13621 gctgaccgcc gacgattttt tgcgttcctt cgcgcaggaa actttccgcc ataattccag
    13681 caatctccgt agagccattg cggatttgct gacaaatatc ctcacaaact tctaactggc
    13741 gacggtgctg cttctggcag ttaccgtggc tgaaatccac caccagatgt tcaggtaaat
    13801 caaactcgtg cagcgtatcg caggctgcgg cgatatcatc ggcatgataa ttcggttttt
    13861 tgccgccacg cataataatg tggccatacg ggttgccgct ggtctgatag atggtcatct
    13921 gaccattttt gtctggcgag aggaacatat ggctggcgcg ggctgcgcgg atagcatcca
    13981 cggcaatccg cgtgttgcca tcggtaccat ttttaaaacc taccggacag gagagtgctg
    14041 aagccatttc gcggtggatc tgactttcgg tagtacgagc gccaatcgcg ccccaactga
    14101 ttaaatcagc aataaactga ccggttacca tatcgaggaa ctcagtcgcg gtagggacac
    14161 ccagctcatt tacctgtaaa agtaatttgc gcgccagctc cagaccgtga tttacccgat
    14221 agctgccgtt taaatccgga tcggagatta gccctttcca gccgacaaca gttcgtggtt
    14281 tttcaaaata ggtgcgcatt acgatttcca gccgtgactg gtactggttg cgcagcgact
    14341 gcagacgggt ggcgtactcc attgcagcgg tgagatcgtg gatcgagcag gggccaataa
    14401 tgaccaacag tcgcttatct tcaccattca gtattttttc aattctgcgg cgggagtcgg
    14461 tgacatgggt ggcgacgcca ggcgttacgg gataccgtag cgcgagttcg gcgggcgtta
    14521 ccaggctctc aatacgcgca gtacggagtt cgtcagttct gttcattaca agtctcagaa
    14581 atttgttgct tctacggcag gactaccgga aagtgatgcg catcacgata aaccaatcgc
    14641 agctgatttc aagtgcgagg tgtgtcacac ctcgccgggt ggttagcatg ataacaaaaa
    14701 aacaaaccaa tgcactagtt ctctagtaca ttcggcgact aaggcccatg atatcgagga
    14761 tcttggtggc aatctcttct accgaataat tggtactgtt aatccacggg atctgatttt
    14821 tacggtacaa ggcttccact tccgcgactt ccatcctgca ctgacgaagc gaggcatagc
    14881 gactgttctc ccgacgttcc tcgcgaatcg ccgccagacg ttccgggtcg atagtcaggc
    14941 cgaacaattt atgctgaagc ggtttgagcg acgcgggtag caccagatta tccatatcgt
    15001 cggcaataaa ggggtagttt gccgcgcgga taccaaattg cattgccaga tacagactgg
    15061 tgggggtttt accacagcga gaaacaccga gcaggatcac ctgagcctga tccagattgc
    15121 gcaacgaaat gccgtcatcg tgggcgaggg tgtaatcaat cgccgcaatg cgcgcatcat
    15181 atttattgag attattaggg ttaaggccat gggtacgatg agcaatcggc gttggatcca
    15241 gtttcatctc ttgttgtagc ggggcaacca gcgcctgaac gatatcctgg caaaagcctt
    15301 cactttgcaa gatgatggcg cgaatctccg gcaacacgat ggagtagaag accagcgggc
    15361 gcacgcctgt ctggtgataa attgcgtcaa tctgatcctt cactgcccgt gcacggctct
    15421 cattttcgac aaacggcagc gtgatgctgc tgatagtgac gggaaattgt gacattactg
    15481 cgtgtcctaa tacctccgca gttattgccg taccatcaga aatataaaaa acgtggcgat
    15541 caacagcatt atccattttg ttcttcccgt gaagcagaca taattctatg aaagcataaa
    15601 ttaaaagcgc acagaagcgt aggacgtaat gtctggttta taaaatgaac cttcatttta
    15661 ttttttataa aaacagcatt tcatttttat ggtttcgttt ataccgatgg tttatgtgga
    15721 aattgtcgaa tagagcaggt ttgcgcaacg ctgggatcag tcttaaaaag taaaaaaata
    15781 tatttgcttg aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc
    15841 agaaatgtgt ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg
    15901 gctcgtcacc gctggtgctt tggtataacc aactcggcat gaatgatgta gacagggttg
    15961 ggggcaaaaa tgcctccctg ggtgaaatga ttactaatct ttccggaatg ggtgtttccg
    16021 ttccgaatgg tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg
    16081 taaaccagcg catttatgaa ctgctggata aaacggatat tgacgatgtt actcagcttg
    16141 cgaaagcggg cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg
    16201 aaaacgccat acgcgaagcc tatgcacagc tttccgccga tgacgaaaac gcctcttttg
    16261 cggtacgctc ctccgccacc gcagaagaca tgccggacgc ttcctttgcc ggtcagcagg
    16321 aaaccttcct caacgttcag ggttttgacg ccgttctcgt ggcagtgaaa catgtatttg
    16381 cttctctgtt taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg
    16441 gcgtggcgct ctccgccggt gttcagcgga tggtgcgctc cgacctcgca tcttctggcg
    16501 tgatgttctc cattgatacc gaatccggct ttgaccaggt ggtgtttatc acttccgcat
    16561 ggggccttgg ggaaatggtc gtgcagggtg cggttaaccc ggatgagttt tacgtgcata
    16621 aaccgacact ggcggcgaat cgcccggcta tcgtgcgccg caccatgggg tcgaaaaaaa
    16681 tccgcatggt ttacgcgccg acccaggagc acggcaagca ggttaaaatc gaagacgtac
    16741 cgcaggaaca gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac
    16801 aggccgtaca aattgagaaa cactacggtc gcccaatgga tattgagtgg gcgaaagatg
    16861 gccacaccgg caaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc
    16921 aggtcatgga gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta
    16981 tcggtcatcg catcggcgcg ggtccggtga aagtcatcca tgacatcagc gaaatgaacc
    17041 gcatcgaacc tggcgacgtg ctggttactg acatgaccga cccggactgg gaaccgatca
    17101 tgaagaaagc atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca
    17161 tcgctcgtga actgggcatt ccggcggtag tgggctgtgg agatgcaaca gaacggatga
    17221 aagacggtga gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg
    17281 agttgctgga atttagcgtg aaaagctcca gcgtagaaac gatgccggat ctgccgttga
    17341 aagtgatgat gaacgtcggt aacccggacc gtgctttcga cttcgcctgc ctgccgagcg
    17401 aaggcgtggg ccttgcgcgt ctggaattta tcatcaaccg tatgattggc gtccacccac
    17461 gcgcactgct tgagtttgac gatcaggaac cgcagttgca aaacgaaatc cgcgagatga
    17521 tgaaaggttt tgattctccg cgtgaatttt acgttggtcg tctgactgaa gggatcgcga
    17581 cgctgggtgc cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga
    17641 acgaatatgc caacctggtc ggtggtgagc gttacgagcc agatgaagag aacccgatgc
    17701 tcggcttccg tggcgcgggc cgctatgttt ccgacagctt ccgcgactgc ttcgcactgg
    17761 agtgtgaagc agtgaaacgt gtgcgcaacg acatggggct gaccaacgtt gagatcatga
    17821 tcccgttcgt gcgtaccgta gatcaggcga aagcggtggt tgaagaactg gcgcgtcagg
    17881 ggctgaaacg tggcgagaac gggctgaaaa tcatcatgat gtgtgaaatc ccgtccaacg
    17941 ccttgctggc cgagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgata
    18001 tgacgcagct ggcgctcggt ctggaccgtg actccggcgt ggtgtctgaa ttgttcgatg
    18061 agcgcaacga tgcggtgaaa gcactgctgt cgatggctat ccgtgccgcg aagaaacagg
    18121 gcaaatatgt cgggatttgc ggtcagggtc cgtccgacca cgaagacttt gccgcatggt
    18181 tgatggaaga ggggatcgat agcctgtctc tgaacccgga caccgtggtg caaacctggt
    18241 taagcctggc tgaactgaag aaataaaata aatccccggc ggcgttcagt cgccggggtt
    18301 atgtgatccc cgaagatgaa acttattcaa tctcttcaca gacatcctgc gttaatcgcc
    18361 gtataatatc ttttcttaac aaaaactttt gtattttatc tgaggcagtg cgcggtagtt
    18421 tttcgattac cacgatatgt tcaggatatt tatattttgc gacccgttta cggctaaaaa
    18481 aagccgccat ctcttccagc gataatgaat gatgcggcgc tttcagcacg acataagcgc
    18541 atgatcgttc acctaaacgt tcatcgggca ttgcaaccac acaggcatcg tgaattttag
    18601 gatgctgcaa taaaatatct tccacttcac ggctgctaat attttcgccg ccgcggacaa
    18661 taatatcttt tttgcgtccg gtaattttta tatagccagc ctcatccata cggcagagat
    18721 cgccgctgta ataccagcct tcttcatcca gcgcacgggc ggttaattca ggttcatcaa
    18781 aataccccat aaacacattg gggccacgcg atgcttcttc accttcgcaa cctggcggta
    18841 aggtcttgcg tgcgtcatcg accactttaa tctctacacc tgcggcagcg taaccatcgg
    18901 tatgcataaa gcgcgacaaa gaattatcga gattcaccac cgcatgcggc gaactttctg
    18961 tggaaccata aacacttaat aatttaatgc tgcgctgctg gcattcgcgc gccacttttt
    19021 tggggattgt ggtaccgccg caaagaaaga agcgcagcgc tgaaaggtct gcgggttgtt
    19081 tctctagtaa attcaaaaga tcatagacaa acggcgttgc gcctagcata caggtgcaac
    19141 gctgctgctc aagcagcgcg agacacgcat caggagtgaa aatatctaac aacacgctgc
    19201 gagcgccaat taagaatggt gccgttacgc catgcagaaa gcccgttgcg tgaccaagtg
    19261 gcgcaggcat cataaagaca tccagccagg tcagattcag ccgcgcgcaa taagcccgct
    19321 cactggcgag aatattgtta tgcgttagca tcacgccctt tggcagaccc tcggttccgg
    19381 aggtaaacag caccgcagct aattcatcgc cgtgggtcgt tatcgccgtg gtcagtggga
    19441 tattgtcggc gataatctga cttaatgaga gggaagaggt ggcgggagcc agtttgtcca
    19501 cgccgacaat ttgttgtagt tgtggaagct gattttgcag cggcaggatt aaatctaccg
    19561 gacgcgtttg tttaaacaac gtcggtgcaa agaacatttt tgcctgacac ttattgagca
    19621 cccacaccag ttctgcttcc cgccaggaag gcaacagcgg cacggaaacc gcaccgattt
    19681 tcaggcaggc aagatagata acggtaaatt cacaccagcc aggcagttga aatgcgatgc
    19741 gatcgcctga ttcaataccc ttcgctaaca tccagtttgc cagacagctc gcggcgtgat
    19801 cgagcgcgct ataggtgtac gatgcaccat gattatcgac cacggcaatt ttgtctggca
    19861 tcgcacgagc ggtctgctgc cagtaatcgg ccagtgaagc atcgccccat agcccttgct
    19921 gacgatacgc cgcacgacgt tgttcgttaa acgttaatgt cactttcatt ttggactctc
    19981 gaaacagaac atcaggcccg agttgcgggc ctgtgggatg catgattagc cgtagcgaaa
    20041 atcaataccg aaggtgcctg cgggatattg ccactgttcg agaatagtgt taccgcacag
    20101 cacccgacag gtgccgcact ccagacaacc ggcgggatca aaatgaatgt ttcctacgtc
    20161 atcctgctta taaagtccgg cagggcaggc tttcattaat ttacggaatt cattgatatc
    20221 gggattttcc gtcagaatga tatgcgggtg gccctcatca acatggaatt tattgacgcc
    20281 taatttgatg tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg
    20341 ccatctttca gcaagttgat cagcccaatt ttcttcgcgt gtcccatgat cattttgcgt
    20401 accggctggt ttggtttgcc gtcaatggtg aacatctcgt tcatgatgtc ggctaccatt
    20461 cgtgggtatt ggctaaacag gcgcgggttt tccatcagcg ccgggatctt gcgaaaatgc
    20521 tgcatatcac gcatgacgca gctttgttcc agctcacgtt tgtattgcgc cagactgttg
    20581 gcggagaaat ccgcgcgttc tttggcggcg atcaccgttg tggcggcagc ctgagccgat
    20641 gcaatggcta aatccatgcc gcggaccgta aaacccaaat tcaggcagaa gcctgcggcg
    20701 tcaccaacga tcatcacgcc ctcgttaacc agttgcggca ccattgccag accgccttcc
    20761 ggcaccatat gcgcggaata ttcaagcagt ttgccgccgc taatcagcgg gcgaatggcg
    20821 gggtgttgtt taaaatcttc cagcatttgc ggcacgcttt tttgcgcatg ggcgatatca
    20881 cccaatccac aaaccagccc caaggatatg gaatccttgt tggtatagag aaatcccccg
    20941 cccatcaggc cgtcagaagg ggaaccggca aacagccagg cggcaccttc ctcgcccgta
    21001 atattaaagc gatcgttgat ctgttctggt gtgaggccaa taacctcttt aacaccaaca
    21061 gcgtaatgat gcggatcgga agcgggaacc attcccagcg agcggccaag catcgagtta
    21121 acgccatcag ccagaatcac cacattcgct tcgagaatat catccccagc ctgcacgcca
    21181 gtgaccttgt ttccttcacg aaccagcgcg tcgacgcgaa cgcctgggat aaactgcgcg
    21241 cccgcctgct cggcttgttc catcaaccac gggtccagac gattacgcaa tacggtataa
    21301 gatgcgtgtt gaggaacatc tggttgctcg cggtgaaaat cgagggtaac ggcgctttct
    21361 tcggttaaga aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc tgatactgca
    21421 aagcctggaa tgattgcttc aagtgtgtgg gcataaagac gcccgccggt catgttttta
    21481 catccggcac tgtcgccgcg ttctatcacc aggacatcca gcccggctcg tgccatgacc
    21541 agtgcggcaa cgctaccagc aacgcccgca ccgaccacaa tggcatcaaa tttgtcatcc
    21601 gacatgcgtt atgctccctt cctgctgcgt ttacagcggg agatgtggtt gaaaggggaa
    21661 ttagcggctc aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc
    21721 gtagtcggca tagttgaaga ttggcgcatt tttatcttta ttgatggcga caatcacttt
    21781 tacgccgttg ccgccaacca tatgttggat ctgcccggag atccccagcg tcaggtagag
    21841 atcggatttc agcaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc
    21901 gccttcggca attggacgtg aacagccgac ttcagcattc agcaccgccg ccagttcgtg
    21961 gaccattttc aggtcatcct gcgccgccag accacgaccg acgccaacca cacgttttgc
    22021 tttgctcagg tccacgctgc ttgcggcttt cgcacggcgt tcgcgacaga gaatttcatg
    22081 acgcggagca acataaggta ccgtttctgt cgggcactga tgagaggtat cactggtgca
    22141 cggttcctga acaccgggtg caagggtaat gatcgccagc gggctgttga tcttttccta
    22201 agcgaacgct aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc
    22261 caccgccgtg gcatcgttca ccagcgccgc attcagttgc acacttaacc gtgctgccag
    22321 agctttacca cgtttcgtcg cggccaacag caacatagcg gggtgtttat ctttcagcag
    22381 ggcagcaatg ctttcggcgt aattttcagt gcgttgcagc gcgtcgtttt gctcaagaac
    22441 ataaatacat tttggaccat aaggcataac tgcctgcgcc tggtcggtat tttgtacaat
    22501 ggcatacacc tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg
    22561 attatcgcta aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc
    22621 agttcagggc tttcttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat
    22681 cgagaatgat gtgcttacgt tctgtttgcg gcggtacgcg aatgccgacc agttcagcaa
    22741 gtggcgcgct ctggctccag tcaatatcac ttgcctgcca ctgatttacc ggttttttac
    22801 ccgcgccgag aatggctttc atcgaaggaa tgcgtggcac gttaatatcg gaggtgacgc
    22861 agagcacggc tggaacagag agttcaataa cttcaacatc atcttcaagc gtgcgttcaa
    22921 tcaccagtgt attgccctga cgctgaatag cactcactgc attaatcacc ggaagttgca
    22981 gaatttctcc gaccagcaag ccaacctgct gggcataaag gtcgccggaa ccttcaccaa
    23041 agatcagtaa atcgaagccg atcttttcaa ttgctgccgc cagcgctttt gcggtatcga
    23101 gaggcagtgc atgttcaagt tgcgcatcct gcaccaaata caggctgtgc ggcccgcggg
    23161 atagcacgtc tttgcgcact ttcgagttct gcaacaatga gccgccaacg gtcagcgcgg
    23221 ctatctcatc gtcatctgtt gcgagctggc ttgcagcttc aatggcattg agatcgaact
    23281 ggctgatttt ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg acaacaatgt
    23341 cctgttcttc aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc
    23401 gcatacggaa ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg
    23461 tgcggggggt tcgacggagc aagctgtttt ccttgccaac aatct
//
LOCUS       DAJVPP010000063        22607 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVPP010000063 DAJVPP010000000
VERSION     DAJVPP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.063"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            18..227
                     /gene="fumD"
                     /locus_tag="OQM34_002491"
     CDS             18..227
                     /gene="fumD"
                     /locus_tag="OQM34_002491"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS1452894.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            683..1309
                     /locus_tag="OQM34_002492"
     CDS             683..1309
                     /locus_tag="OQM34_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS1452895.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1330..3431
                     /locus_tag="OQM34_002493"
                     /pseudo
     CDS             1330..3431
                     /locus_tag="OQM34_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3444..4082
                     /locus_tag="OQM34_002494"
     CDS             3444..4082
                     /locus_tag="OQM34_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS1452896.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4146..4814
                     /locus_tag="OQM34_002495"
     CDS             4146..4814
                     /locus_tag="OQM34_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1452897.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4811..5596
                     /locus_tag="OQM34_002496"
     CDS             4811..5596
                     /locus_tag="OQM34_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS1452898.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5600..6412
                     /locus_tag="OQM34_002497"
     CDS             5600..6412
                     /locus_tag="OQM34_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452899.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6424..8028)
                     /locus_tag="OQM34_002498"
     CDS             complement(6424..8028)
                     /locus_tag="OQM34_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCS1452900.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(8154..8459)
                     /locus_tag="OQM34_002499"
     CDS             complement(8154..8459)
                     /locus_tag="OQM34_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1452901.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8567..8643)
                     /locus_tag="OQM34_002500"
     tRNA            complement(8567..8643)
                     /locus_tag="OQM34_002500"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8607..8609),aa:Val,seq:gac)
     gene            complement(8648..8724)
                     /locus_tag="OQM34_002501"
     tRNA            complement(8648..8724)
                     /locus_tag="OQM34_002501"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8688..8690),aa:Val,seq:gac)
     gene            9032..10288
                     /locus_tag="OQM34_002502"
     CDS             9032..10288
                     /locus_tag="OQM34_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1452902.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10329..11702)
                     /gene="mdtK"
                     /locus_tag="OQM34_002503"
     CDS             complement(10329..11702)
                     /gene="mdtK"
                     /locus_tag="OQM34_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1452903.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11917..12558
                     /gene="ribE"
                     /locus_tag="OQM34_002504"
     CDS             11917..12558
                     /gene="ribE"
                     /locus_tag="OQM34_002504"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1452904.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12598..13746)
                     /gene="cfa"
                     /locus_tag="OQM34_002505"
     CDS             complement(12598..13746)
                     /gene="cfa"
                     /locus_tag="OQM34_002505"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1452905.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(14037..15248)
                     /locus_tag="OQM34_002506"
     CDS             complement(14037..15248)
                     /locus_tag="OQM34_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1452906.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15361..16293
                     /locus_tag="OQM34_002507"
     CDS             15361..16293
                     /locus_tag="OQM34_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1452907.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(16290..17315)
                     /gene="purR"
                     /locus_tag="OQM34_002508"
     CDS             complement(16290..17315)
                     /gene="purR"
                     /locus_tag="OQM34_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1452908.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17614..17703
                     /gene="ynhF"
                     /locus_tag="OQM34_002509"
     CDS             17614..17703
                     /gene="ynhF"
                     /locus_tag="OQM34_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1452909.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17869..19038
                     /locus_tag="OQM34_002510"
     CDS             17869..19038
                     /locus_tag="OQM34_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1452910.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19184..19765)
                     /gene="sodB"
                     /locus_tag="OQM34_002511"
     CDS             complement(19184..19765)
                     /gene="sodB"
                     /locus_tag="OQM34_002511"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1452911.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19893..20720)
                     /gene="mepH"
                     /locus_tag="OQM34_002512"
     CDS             complement(19893..20720)
                     /gene="mepH"
                     /locus_tag="OQM34_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1452912.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            21055..21402
                     /gene="grxD"
                     /locus_tag="OQM34_002513"
     CDS             21055..21402
                     /gene="grxD"
                     /locus_tag="OQM34_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS1452913.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21441..21941)
                     /gene="tnpA"
                     /locus_tag="OQM34_002514"
     CDS             complement(21441..21941)
                     /gene="tnpA"
                     /locus_tag="OQM34_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1452914.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            21949..>22607
                     /locus_tag="OQM34_002515"
     CDS             21949..>22607
                     /locus_tag="OQM34_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1452915.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT         5857 a         5255 c         5847 g         5648 t
ORIGIN      
        1 ataaataggt ataaagaatg ggaaatcgga caaaagagga tgaactgtac cgagaaatgt
       61 gcagagttgt cggtaaagtt gtgctggaaa tgcgcgatct ggggcaggaa ccaaagcata
      121 ttgttatcgc aggcgtatta cgtacagcat tagccaataa acgcattcag cgcagtgagc
      181 tggaaaagca ggcgatggaa accgtcatta acgcactggt aaaatgacca ttcaggatga
      241 ggcggtaagc ttcatcctga tattcttgct tcagttgcgg tccagttaat cattctaaat
      301 aacaactgct gaaattatac gaatttcggt atgtatttta atttatagcg ataatgttat
      361 ttttattttc tgcaatgaac gtcacaaatg attgtcgctt aagttaaatt tatttcaaat
      421 gataataaat atcagttcgc ttataatttc ttgctttgta attttaattg ataacgatca
      481 atgttaaggg ctcagaataa tcacattatt ttttctccta accataaagg attagtttat
      541 cggcgttaaa tcttttctca tacactatcg ggttagggaa aatatctggt gttggggaca
      601 gagtcagcag cgtattagtt gaatgcttaa tatcaggctg ctgaattgtc agcactgtgt
      661 ttgcgaagag gatgcgaaat aaatgaaccc ggttgatcgt ccactattag atattggctt
      721 aacgcgatta gaattcttgc gtatatcagg aaaaggcctt gctggtctaa caattgcccc
      781 tgcgttactg tcacttttgg gttgcaaaca ggaagatatt gatagcggca cagtagggtt
      841 gataaataca cccaaagggg tgctggtgac ccagcgagcg cgatgcacgg gttgccaccg
      901 ctgtgaaatc tcttgtacca acttcaacga tggctcagta gggacattct tctcccgtat
      961 caaaatccat cgcaattatt tctttggcga caacggggtt ggctctggcg gcggcctgta
     1021 tggcgatctc aactataccg cggacacctg ccgtcaatgc aaagagccgc aatgcatgaa
     1081 cgtctgcccg attggtgcga ttacctggca gcagaaagaa ggctgtatta ccgtcgatca
     1141 taaacgttgt attggctgta gcgcctgtac tacggcatgc ccgtggatga tggccaccgt
     1201 aaataccgaa agtaaaaaat cctcgaaatg tgtgttatgc ggtgaatgcg caaacgcctg
     1261 cccaacaggg gcgttaaaaa ttatcgagtg gaaagatatt actgtgtgaa tcttgcaaag
     1321 gaaaaaatca tggctaacgg ttggacaggt aatatattaa gagtcaatct cacgacagga
     1381 aatattaccc tcgaagattc cagtaagttt aaaagttttg tcggtggcat gggcttcggc
     1441 tacaaaatta tgtatgacga agtaccgcta ggcacgaaac ctttcgatga agcgaataaa
     1501 ttagtctttg ctaccggccc attaactgga tctggtgccc cctgtagttc tcgcgtaaat
     1561 atcacctcac tttctacttt taccaaagga aatttagtcg cgatgcccat atgggtggct
     1621 tttttgcagc gcaaatgaaa ttcgctggat acgacgtcat tattatcgaa gggaaggcga
     1681 aatcaccggt atggctgaag attaaagatg ataaagttag cctggaaaaa gccgatttct
     1741 tatggggaaa agggacgcgc gcaacgacgg aagaaatttg tcgattgacc agcccggaaa
     1801 cctgtgtggc ggctattggt caggctgggg aaaaccttat tcctctctct ggcatgttga
     1861 atagccgtaa ccacagcggc ggtgcgggaa ctggcgcaat aatgggttcg aaaaacctga
     1921 aagcgattgc ggttgaaggg acgaaagggg tcaacattgc cgatcgtcag gagatgaagc
     1981 gtctcaatga ttacatgatg actgaactta ttggtgcgaa taacaaccat gtcgtgccaa
     2041 gtacgccaca atcgtgggca gagtattcag atcccaagtc acgctggaca gcacgtaaag
     2101 ggctgttttg gggcgcggct gaaggtggtc cgattgaaac gggtgaaatt ccgccaggca
     2161 atcagaatac ggtcggcttt cgtacctata aatccgtttt tgacttagga ccggcggcag
     2221 agaaatacac agtaaaaatg agcggctgcc actcttgccc gatccgttgt atgacccaaa
     2281 tgaatattcc tcgggtgaaa gagtttggcg tgcccagcac aggtggtaac acttgtgtag
     2341 caaactttgt ccataccacc atcttcccga acgggccgaa agattttgaa gataaagacg
     2401 atggtcgtgt gattggtaac ctggttggtc tgaatttgtt cgatgactac ggcctatggt
     2461 gtaactacgg gcagttgcat cgcgacttta cttattgtta cagcaaaggt gtgttcaagc
     2521 gtgttctgcc agctgaagag tatgcagaaa ttcgctggga tcaactggaa gcgggtgacg
     2581 ttaacttcat taaagatttt tactaccgtc tggcgcatcg tgtgggtgag ctgagtcacc
     2641 tggctgatgg ttcatatgcc atcgcagaac gctggaattt gggtaaagag tactggggct
     2701 acgcgaaaaa taaactctgg tcgccgtttg gctatccggt tcaccatgcc aatgaagcgt
     2761 cagcgcaggt cggttccatt gttaactgta tgttcaaccg tgactgcatg acgcataccc
     2821 atatcaactt tattggttcc ggcttgccat tgaaactgca acgtgaagtg gcgaaagaac
     2881 tttttggttc tgaagatgct tacgatgaaa ccaaaaacta cacgccaatc aatgacgcaa
     2941 aaattaaata tgcaaagtgg tcgctgttgc gggtctgttt gcataacgcc gtcactctgt
     3001 gcaactgggt ctggccaatg accgtttcgc cgctgaaaag ccgtaattac cggggcgatc
     3061 tggcgcttga agccaaattc ttcaaagcga tcaccggcga agagatgact caggaaaaat
     3121 tagatttagc tgcagagcgt atctttacgt tgcatcgtgc ctacacggta aaaccgatgc
     3181 aaaccaaaga tatgcgtaat gaacacgatc ttatctgttc ctgggttttc gacaaggatc
     3241 cgcagatccc ggtctttact gaaggtactg acaaaatgga tcgtgacgat atgcatgctt
     3301 cgctcacgat gttctacaaa gaaatgggct gggacccaca gcttggttgt ccaacccgcg
     3361 aaacattgca gcgtctgggg ctggaagata ttgccgccga tctggcagca cacaatctac
     3421 tgcctgcgta aggaatgggt aagatgaacc atcaggatga attgccgtta gcgaaagtca
     3481 gcgaagttga cgaggcgaag cggcaatggt tgcaggggat gcgtcacccc gttgatacgg
     3541 taaccgaacc agaacctgct gaaatcctgg ctgagttcat tcgccagcat tctgcggctg
     3601 ggcagctggt ggcgcgcacc gtgtttctct cgccacccta ttcggttgca gaagaggaac
     3661 tgtcagtttt gctggaaagc attaagcaga acggagacta cgcggatatc gcttgtatga
     3721 cgggaagtca ggatgattac tattactcaa cgcaggccat gagtgaaaac tacgcggcga
     3781 tgtctttgca agttgtggag caggacattt gtcgcgccat tgcacacgcc gttcgttttg
     3841 agtgccagac gtatcctcgt ccttacaaag tcgccatgct gatgcaagcg ccatattact
     3901 tccaggaagc gcagattgaa gccgcgatcg ccgcgatgga tgttgcacct gaatatgccg
     3961 atatacggca ggtggagtca tcgacggcgg tgttgtactt gtttagcgag cgatttatga
     4021 cctatggcaa ggcttatggc ttatgcgaat ggttcgaggt agagcagttt cagaacccct
     4081 gaaatttcag ggagatgtca tggatagggt ggacagttgc agccactgca ttaggggata
     4141 atcagatgtc cttcactcga cgcaaatttg ttctggggat gggaacagta atctttttta
     4201 ctggttctgc gtcttctctg ttagcgaaca cgaggcaaga aaaggaagtt cggtatgcca
     4261 tgattcatga cgagtcacgg tgcaatggct gtaatatttg cgcccgcgca tgccgtaaaa
     4321 ctaaccacgt ccccgctcag ggaagccgtt tatcgatagc acacattcca gtcaccgata
     4381 acgacaacga aacgcagtat cacttttttc gccagtcatg tcagcactgc gaagatgctc
     4441 cgtgcatcga cgtatgcccg acgggggcat cgtggcgtga tgagcagggg atcgtgcggg
     4501 tagaaaaatc gcagtgcatc ggctgcagtt attgcattgg cgcttgtccg taccaggttc
     4561 gctaccttaa ccctgtcact aaagtggcag ataaatgtga tttctgcgct gagtcccgac
     4621 tggcaaaagg ctttccgccc atttgcgtca gcgcctgtcc ggaacacgca ttgatctttg
     4681 gtcgcgaaga tagcccagaa attcaggcgt ggttgcagga taataaatac tatcaatatc
     4741 agctacctgg tgccggaaaa ccgcatctgt atcgccggtt cggtcaacat ttgattaaaa
     4801 aggaaaatgt atgaacccgt cgcaacatgc tgaacagttt cagagccagt tagcgaacta
     4861 tgtgccacag ttcactcccg aattttggcc ggtgtggttg atcattgccg gagtattgct
     4921 ggttgggatg tggttggtgc tggggctgca cgccttgctt cgtgctcgtg gcgtgaagaa
     4981 atcagccacc gatcatggtg agaaggttta tctttacagc aaagcggtca gattatggca
     5041 ctggtcgaat gcgttactct ttgtattgtt gctggcaagt gggctgataa atcactttgc
     5101 gatggtgggc gcaactgcgg ttaaaagtct ggttgcggtg catgaagttt gcggattttt
     5161 gttactggca tgctggctcg gctttgtgct gatcaatgcc gttggggata atggtcacca
     5221 ctatcgcatt cgtcgtcagg ggtggctgga acgagcggca aaacaaacgc gattttattt
     5281 gtttggcatt atgcaggggg aagaacatcc tttcccggca acaacacagt ctaaatttaa
     5341 tcccttacag caggtcgcct atgttggtgt catgtatgga ttgctgccgt tgttactatt
     5401 gacggggctg ttgtgtctct atccgcaagc cgtgggagat gtgtttcctg gcgtaagata
     5461 ctggttattg caggcgcatt ttgctctggc atttataagc ctctttttta tcttcggtca
     5521 tctttatctt tgcaccacgg ggcgtacgcc acacgaaacc tttaaaagca tggtcgatgg
     5581 ctatcaccgg cactaacgaa tgatcattac acgagcagac ctgcgagaat ggcgtatcgg
     5641 agcggtgatg tatcgctggt tcctgcgcca tttcccacgc ggcggtagtt atgccgatat
     5701 tcgtcatgca ctgattgagg aaggatatac ggactgggcg gagagtctgg ttgagtatgc
     5761 gtggaaaaaa tggctggcgg atgaaaactt cgcgcatcag gaagtttcct ctatgcaaaa
     5821 actggccaca gaccccggag agataccatt ttgcagtcag tttgcccgca gcgacgatca
     5881 tgctcgcatc ggctgttgtg aggataacgc ccgaatcgca accgctgggt atgccgcgca
     5941 aattgccagt atgggttata gcgtgcggat tggaagtgtc ggctttaaca gtcacattgg
     6001 cagctccggt gaacgcgcac gggttgctgt aaccggtaat tcttcgcgga taagcagcgc
     6061 tggagatagt agtcggattg cgaataccgg aatgcgagta cgcgtttgta cattgggtga
     6121 acggtgtcat gtcgccagta atggtgacct ggtgcaaatc gccagctttg gcgcaaacgc
     6181 cagaattgcc aacagcggcg acaatgtaca catcattgcc agcggtgaaa attcaacggt
     6241 agtgagcacg ggcgtggtcg actcaattat tctcgggcta gggggaagcg ccgcactggc
     6301 atatcatgac ggcgaaagag ttcgcttcgc cgtcgccatt gaaggtgaaa acaatattcg
     6361 aaccggagtc cgctatcgtc ttaatgaaca gcatcagttt gttgagtgct gatagcttta
     6421 cctttaatca aacgaaagac gccgacgagc acgggatgca ggctttacga ccgcccgagc
     6481 catcgcctca ccaatttctg cacatgccgt aagtccctga acgaaaggct ggtcgtgcat
     6541 caaccagggc aacgcgccga acgctacgcg tccacgaata tcttcgggtt gctgtaacgt
     6601 ataatcttcg ccaacatcag ggatttcatc ccctgttttc tgtaattgct cgcgtagccc
     6661 agggaaagga atatctttca ctttaagcgg acgctgtccg cgggcatcaa taaaaacgtc
     6721 aaacgagtag ctgttgtctt ccgttttcag gacggtgcgc gactcattaa tttccatttc
     6781 gtagtcttca ccgagggcga gaatatgaat gatccccgct tcacgtaagg ccagtagtcg
     6841 acgaatagac tctgaaggga ttgccgcata attatcgatg aaaaccctgg caaggccttt
     6901 actgaaccgt ttatggtcgt gttcattcag atgaggaaca atttcctgta cggcttcatg
     6961 caggcgcaga atgacataac gccagggaac agtatgtttt tctcgtttat tgcgttcgac
     7021 ttcctggaga tttttttctg cccagtcaaa tgggtcgcgt tgtttgcgct cggcaaacca
     7081 ggcttgagca aaggaatcga cattcaggct ctctaaggct atgcgttggc tccagtctgg
     7141 atcagcaaac ttgatttcct ctactatcaa tctaaatacc cgatccaaaa ggccctcttc
     7201 acctttttga atctcagcat ttaatgcctg atcggtgacg atgtgtaagg gctcgtaggg
     7261 aatagggcaa tagaaatcgg cttcgggtaa aatacccgtg cgcgacatca acgtaatatt
     7321 tagcttttca cttgcgttat cgcggtgaaa aacgacgtgt tgtttatcat cttcaatgaa
     7381 cgaaccatgc tgaatagcca ctgccattgc cgcatccagt ccgctcaagg atgttcccat
     7441 aatacccacg ttacacgcat cgacctttgc ttccatcagg cctgaccacg ggctgggaaa
     7501 atacgttctg gttgcttctt cttcatcagg ccagacgtga cccgtggcaa tcaccgctaa
     7561 atcaaacgtc tcgctgggta aatcctgatt tgtagcgagc atgacgccag catttgtaat
     7621 ttgcagatcg gtaacctggc atgattcata aacagccact gcaaattttt gctgtcgtgc
     7681 ctggtctact aatcgtaaaa attgatcgcg gaaatattcg cccagcagaa ttcgcggtaa
     7741 aaactgacga tcgtgcaagg tttctttttt aacgccataa cgctggagat ggctggcttc
     7801 ttgcttttgt agccattcga gatacgtaca attaatcggc ggtatttcaa tactggcaat
     7861 atttgccagc atcatttttg agttttcctc atcactgtat ggcatcccga caccggcctc
     7921 gtcagcctgc tcgaagatag aaatagaaag tggagtttgt tgctgtagaa gcgagaataa
     7981 ggtgtagatc cccgtaggcc cggcacccac aatagcaatt tttttcattg ttacttacct
     8041 gtacgcattc tttcgaattt agtgttatca gataagtttg gttgagattt ttagaatttg
     8101 agaaatttca gacagggatg aaaagggcgc taaccgccct tgattaaatt ctgtcaggcg
     8161 agttttgcct gattgatttg actaagtggc tcattgacat cgaaaacttt ggcgacaact
     8221 tcctcaacgc cgagattttt cagtcgggca gtatgttttt ccagataggc aagggcgctt
     8281 ttttcatcag tgaaaaggta gatcccaccg gcttcgtggt tcttttcact ttctgtccat
     8341 accttccaca gaaaaccagg ttcctgatta atcgactcag caagtggttt aagctgctca
     8401 gccattgcgt cgccaaatgg gccattaaaa gcaaaatgaa gttgtaacaa agttgccata
     8461 gcgattctcc ttctgataac gcatacgaag acagtgtaaa tcttttaacg tattggatac
     8521 agagaagaaa cagagggaaa aaggaaaaga ccagattaag aaaatctggt gcgtccgagt
     8581 ggactcgaac caccgacccc caccatgtca aggtggtgct ctaaccaact gagctacgga
     8641 cgcaggatgg tgcgttcaat tggactcgaa ccaacgaccc ccaccatgtc aaggtggtgc
     8701 tctaaccaac tgagctatga acgcaacgtt gtaggtgaca acggggacga atattagcgg
     8761 cagagtggaa aggtggcaag aggcaaaacg taattttctg cgctatttcg accgtttgca
     8821 gagcttttaa gcaaattggc tatattttgt tgatttgcaa gggcgatttt tattcaggat
     8881 cgcatttaca tctgatacaa cccaataatt cgtgtatcag agtttaaaca gagttgtttg
     8941 ccaccatctc aaatctgtcg tggataaatc ctccgtagcc tttacactta ggtagcaata
     9001 aaggcacaca gatgcagcaa aggaataaat catgggatct gatgcgaaaa acttgatgag
     9061 cgacgggaat gtgcaaattg ttaagaccgg cgaggtcctt ggcgcgacgc aacttactga
     9121 aggcgagttg attgttgaag ctggcggaag agccgaaaat accgtggtca cgggggctgg
     9181 ctggttgaaa gtggcaaccg gtgggatcgc caaatgcaca cagtacggta acaatggcac
     9241 gctatcggtc agcgacggtg ccattgccac agatattgtt cagtccgagg gaggcgcaat
     9301 tagtctctct acgctcgcta cggttaatgg ccgccatccc gaaggtgaat tcagcgttga
     9361 tcagggttat gcctgcggtt tgttgctgga aaatggcggt aacctgcgtg tactggaagg
     9421 acatcgcgcg gaaaaaatca ttctcgatca agagggcggc ctgttggtta atgggacaac
     9481 ctcagcggtc gtggtagatg aaggtggtga attgttggtg tatccaagtg gagaagccag
     9541 caattgtgag attaatcagg gcggcgtttt tatgctggcc gggaaagcca gtgatacgtt
     9601 gcttgctggt ggcaccatga ataatctcgg tggtgaagac tctgacacta ttgttgagaa
     9661 tggatccatt tatcgtctgg ggacggatgg ccttcagctc tacagttccg gtaagacgca
     9721 aaacctgtcg gtgaatgtgg gggggcgggc tgaagtgcat gccggtacgc tggaaaatgc
     9781 ggtaatacaa ggtggaacag tgatcctgtt gtcacccacc agcgcggacg aaaattttgt
     9841 cgtagaggaa gatcgcgcac cggttgaact gaccgggagt gttgcattac tggacggcgc
     9901 ttcaatgatt attggttatg gcgcagagct gcaacaatca acgattactg tacagcaggg
     9961 cggtgtgttg attctcgacg gcagtacggt aaaaggtgac agcgtcactt tcattgttgg
    10021 taacatcaat ctgaatggcg gaaaactgtg gctgatcact ggtgcggcaa cgcatgtgca
    10081 actgaaagtg aaacgcctgc gcggagaggg agcgatttgc ctgcaaacca gtgcgaaaga
    10141 aatttcacct gacttcatca atgtgaaagg ggaagttaac ggggatatac gcgttgagat
    10201 aacagatgcc agtcggcaaa ctctgtgtaa cgctctgaaa ttacagccag acgaagacgg
    10261 gattggcgca acgctccagc ctgcgtaaaa acacaggcgc aacccgtgaa ggttgcgcct
    10321 tgtctttatt agcgggacgc tcgttgcaga atgatggctg acggcatacg ttgcaggaac
    10381 cgcatacgca gcatcatcat aatggctgcc gatgtcaggc cgataataaa gcctatccag
    10441 aagcctgctg gccccatagg ttcaacgacc agatcggtca gtgccagaat atagccgctt
    10501 ggcaagccca gcacccagta agctgtaaag gtgatataga aaatggaacg cgtatcttta
    10561 taaccacgca aaatcccact gccaatcacc tggattgagt cagaaatctg atataccgcc
    10621 gccagcaaca tcaaatgcgc agccagcgtt acaacctcgg gattgtcgtt gtacaacagg
    10681 gcgatttgct cccgcagtga aaccgtgaaa atggccgtca gggttgccat acagacaccc
    10741 accataagcc cggtccgcgc agcggtttgc gcatccagcg ttgagccctg acccagacga
    10801 taacctacgc ggatagttac cgctgccgcc agcgacattg gaagcacaaa catcagtgaa
    10861 ctaaagttca gggcaatctg gtgtcctgcg acatcaacaa taccgagcgg agacactaac
    10921 agagccacga cggcaaacag tgtcacttca aagaacagtg ccagcgcaat cggcaaaccg
    10981 agttgaatca gtcgtttcat aaccgcaggc tcgggcttag cggtgccttt ttcgttacga
    11041 atatcgcgca tggagcgggc gcgtttaatg taagaaacca tggcaaggaa catgacccaa
    11101 tacaccgccg cagtagccac gccacaacca acgccaccga gctcaggcat accgaaatga
    11161 ccataaataa agatatagtt caccgggatg ttcaccagca ggccgataaa gcccattacc
    11221 atacccggct tggtttttgc cagaccttca cattggttac gggcaacctg gaagaacaga
    11281 tatcccggcg cgccccacaa caacgcacgc agataaccca cggctttgtc cgccagagcc
    11341 ggatcgatgt tttccatgta gcggataatg taacctgcat tccacagcac cagcataatg
    11401 agaacggaaa caaaacctgc cagccagaaa ccttgtcgca cctgatgcgc aatgcgctca
    11461 cgtcgaccgg aaccatttaa ttgcgcgata accggcgtta atgccagcag cagtccgtga
    11521 ccaaagagga tcgccggaag ccagatagaa gtaccgatag cgaccgccgc catgtcggtg
    11581 gcactatagc cgcccgccat cacggtatcg acaaaaccca tcgcagtttg ggcgatttgc
    11641 gcgagaatca ccgggattgc taatgctaat aacagacgcg cttcactgat atacttctgc
    11701 acgtgaacac cttttatttg tagttatatg agagactaaa aagccgcctg aatgggcagc
    11761 aaaaagaaga agtaggggaa attcagtcta ttgtagcgag gatttattaa ttctccagtg
    11821 aaaaaatagc cattcttacc ccttcactgg taagggcgtt ttccacctgc tattgtgctg
    11881 aacagaatgt cttaactgat ttcaggagtt gtaagtatgt ttacggggat tgtacagggc
    11941 accgcaaaac tggtgtcgat tgacgagaaa ccaaattttc gtacgcatgt ggtggagtta
    12001 cccgaccaca tgctggacgg cctggaaacc ggtgcttccg tggcgcataa cggttgctgc
    12061 ctgaccgtga cggaaattaa cggcaaccat gtcagttttg acctgatgaa agaaacgtta
    12121 cgcattacca atcttggcga tttaaaagtg ggggattggg taaacgttga gcgtgcggcg
    12181 aaattcagtg atgaaattgg cggacactta atgtcaggtc atattatgac caccgctgaa
    12241 gtggcgaaaa tattaacctc ggaaaataat cgccagatct ggtttaaagt ccaggatagt
    12301 cagttgatga aatatatcct gtacaaagga tttattggca tcgacggtat tagcctgacc
    12361 gtcggcgaag tcacgccaac gcgtttttgc gtccatttaa ttccggaaac actggaacgc
    12421 acgactcttg ggaagaaaaa acttggcgca cgcgtcaaca ttgaaatcga tccacaaact
    12481 caggcagtgg tagatacggt agaacgtgtg ctggcggcac gagaaaatgc catgaatcaa
    12541 ccaggtacag aagcctgatc tgaactcgcc cccgaagagg gggcgataga atagccttta
    12601 gcgagcgact cgaaggccgt tttcaacacc gcgtgagaac accacctgcc agagctgaat
    12661 atcacgggcg cggaaagcac ctgcgcaggc attcagataa taggtaaaca tccgtttaaa
    12721 gcgttcactg tagttatccg caatttctgg ccatgcggcg aggaatcgtt cataccacgc
    12781 catcaacgta gtatcgtaat cagcaccgaa gttatgccag tcttccatca caaagtgggg
    12841 ttcgctggat tgagcaatct ggcgtacaga gggcaggcaa ccgttcggaa aaatatattt
    12901 attaatccag ggatcaacat tcagatcggt ttttttcgaa ccgatagtat ggagcaggaa
    12961 tataccttcc ggtttcaaat tacgatccac caccgcaaaa taggtatcgt aatttttcgg
    13021 tccgacgtgc tcgaacatcc ccacagaaac aatacgatca aactggtcgt tcaggtcacg
    13081 ataatcttgc agcaaaatgg tgacatccag gcctttacag cgttcctgag ccattttttg
    13141 ctgttcggca gaaatggtga cgccgaccac gcttacgtca tagttagatg ccatgtagtg
    13201 cgctagtccg ccccagccgc agccaatatc cagtacgcgc atccctggtt ttaactgcaa
    13261 tttttcacaa atcattttga gcttcgcctg ctgggcagat tccagattat cggcatcttt
    13321 ccagtaagcg caggaatatt gcatgaaggg atcaagcatg cggctgaaca agtcattacc
    13381 caaatcgtaa tgctccttgc cgactatcca ggcacgtttt ttactctgca gattgaagag
    13441 acgagcgccg gcaatacgca gcgtgtcttt gaaatgatgg gggagttggt tctcgagacc
    13501 tgcgcgtaag actttgctaa aaaacatatc cagtcggtca cattcccacc agccatccat
    13561 ataactttcg cctaacccca aagagccttc ctgcagaacg cgtttaaaaa aatcggggtt
    13621 tttcacacga atatccgccg gggcagaacc gttaatggct ataccggcac ggctaagtaa
    13681 ttcgttggcg atacggtacc agttgtcatc cggtacactg acttcttcta tacacgatga
    13741 actcatagtt tctccatcac tggtcggtga tcagaacctt aaaacagcgt agacgctttt
    13801 tgggctttgt gagaaatctc gcagaaaaaa ccgcgagtct ataatccgac gtagaacaga
    13861 gggaaaggag gaacccttgc cgaaatgacc atccatagtg agtcgggaac gatcctgtcc
    13921 ccgtaaaaac ataatattta tcgtagcttt aacaactcga aattagtata ggccgctaat
    13981 ttcaatcata caacaaaatt ttgttagcag cctaagtata agtatatcga tatagatcag
    14041 tgtgattcgc tatgagcgac ttcggcattg ccatgattcg ggcagtcaac ttcttcacaa
    14101 cgttgcatca tgtaacccag cgcgaccagc actactgttg ataacatcac gctggtggtg
    14161 gtgagcaatg gcgtgctgat actaataagc caggaaacta ccagacttgc gaggaagcac
    14221 agacccagtt gcagagtgtt ctgcaacgct gcggcgcgac cggttgcatg tgggaaggga
    14281 cgcagcgcct gggcgacaac aatagggtag atcgcgccat tggcaatcgc catcacacag
    14341 aatgggatca ggatttcgac cagcgacaca tggctaatga agcccgcagc ccaggtcgca
    14401 atgacgctga cagcaaacag caccagcaac cacggtaata actgcttgcc ttgccatttc
    14461 tgcagtgcgg cgcgacagcc ataaccacca atcagaaacg cgatagtttg cgggacataa
    14521 cttaaaccaa taactgccgg gctgtagccc atttcactaa ggatgaacgg tgaaccggtc
    14581 agccatgcaa aaaaactggc tgaacaggct gcgtatatca gcacgttgcc gcgataggtt
    14641 ttagaacgta gcaggtcggt aaaggtcaga ccatcctgac tattgttacg ggccttcgtc
    14701 gtgggtttga gccagaaaat aggcagaatc agcaccacgg taatggcaaa cagggtggcg
    14761 aaaatcgcct gccaggaaaa atggaccagc agccagcttc ctaacagggg agccagtgcc
    14821 ggagacagac ccaccagcgg catgatggtc gcaaaaatac ggttgacttt ctgtgaagga
    14881 taataatctg tcactaatgc ttgccagata accgccgcgg cgcagacccc cacagcctgt
    14941 acaaaacgca ataccagcag cgtggcggcg ttttctaccc acagcatccc cagactacct
    15001 aacgcaaaaa ttgtcaggcc gattaataac accggtttac gaccataacg gtcggagagt
    15061 ggcccccaca gaagctgggc tgcggcaaaa ccggcaagga acagactaag gctggcactg
    15121 acagcagacg caggcgtttg caggtcggcc tgtatggcgg cgaaagcagg cagatacata
    15181 tcggttgcca gaaaaccgag tacgctcaaa cccgccagcc agactaaaaa tcttttccca
    15241 ggttgcatgt atattctcta aatctctcaa ttaaacaccg ccgcaaagtg tagggagtga
    15301 aattagtgtt gtgaaacggt aatatttgat gactggtttc aaaaattttg caggcagact
    15361 atgtggtcag aatattcact cgaagttgtt gatgcggtag cgcgtaatgg tagttttagc
    15421 gctgcggcac aggagctgca tcgtgttcct tctgcggtca gctataccgt gcgtcagctg
    15481 gaagagtggc tggcggtacc gctctttgaa cgacggcacc gtgatgtgga gctgaccgct
    15541 gctggagcgt ggtttctcaa agaagggcgc tctgttgtca aaaaaatgca gatcacccgc
    15601 cagcaatgtc agcagatagc gaacggctgg cgcggtcagt tagctatcgc agtggataat
    15661 attgtcaggc cagaacgtac acggcagatg atcgttgatt tttatcgcca ttttgatgat
    15721 gtcgaacttc ttgtctttca ggaagtgttc aacggtgtct gggatgcgct ttccgacggg
    15781 cgcgtggaac tggcaattgg cgcaacacgc gcgattccgg taggcggtcg ttatgccttc
    15841 cgggatatgg ggatgctaag ctggagttgc gttgttgcca gtcaccaccc gctggcgttg
    15901 atggatggcc cgttcagcga tgatacgttg cgcaactggc cgtcgttggt gcgcgaagac
    15961 acctcgcgaa cgctacctaa acgtattacc tggttgctgg ataaccaaaa aagacttgtc
    16021 gtacctgact gggaatcatc ggcaacctgt atctcggcag gattatgcat agggatggtg
    16081 ccaacacatt tcgccaaacc gtggcttaat gaggggaagt gggtagcact tgaactggaa
    16141 aaccccttcc cggattcggc atgttgtctg acatggcagc aaaatgatat gtcgcctgcg
    16201 ttaacctggc tgcttgagta tctcggcgat agtgagacgc tgaataagga gtggctgcgg
    16261 gagccggaag agactcccgc aacgggtgat taacgacgat agtcgcggaa cgggccgtca
    16321 gccacggagc ggcgttcaat caaacgcgga tgcacttcaa tggactgcgg ttcttcacgt
    16381 ttgttgacga tacgatccaa cagcatgttg aacgctgttt cacccagcga atcttttggc
    16441 tgatggatcg tggtcagcgc cggcgtaaaa tagcgcgcgt tgcgcacgtt atcataaccg
    16501 atcagcgaaa catcctgcgg gacgcgcagg cccatttcat cagcagcaca aagtgcgccc
    16561 attgccatga tatcgccacc acagaagacg gcagtagggc gatgcggctg cgacaggatt
    16621 tgctgcatgg cgcgataacc ggattcaggt tcaaagtcac cctgcacaat ccagctttcc
    16681 ggcaccttga tcatcgcttc ttccatcgcc ttcataaaac cggcaaggcg gcctgcgccg
    16741 gtgttacgtt ccagcgggcc ggggatgacg ccgatttcgc ggtgaccgcg ttcaatcaga
    16801 taacgcccgg ccatgtagcc gccttcgaac gcgttatcaa tgaccgcatc ggtgaagtca
    16861 gcttttgctt caccccagtc catcaccacc attgggatat ggcgatactc ttccagcatc
    16921 gccagcaacg gctctgggta ctcagaacac atcaccagca gaccatcgac gcgtttttgc
    16981 gccatcatcg acagataagc ccgctgtttc tcaagattgt tccacgcatt acccagaatc
    17041 agggtgtaac ctttctggaa gcaatttttt tcaactgctt caatgatctc ggcaaaatag
    17101 gccgcttcgc tgctggtcgc cagcaaaccg atagacttgg tgtggttaac cttcaggcta
    17161 cgcgccaccg cgctagggga gtagtgtaat tctttaatcg ctgcccacac ggcgttgcgc
    17221 gtttcttcag cgacgaaacg tgttttgttg atcacgtgtg acacagttgt agtggaaacg
    17281 tttgctcgtt tcgctacatc ttttattgtt gccattccat ttcactccag accctaactt
    17341 cagctcctgc aaaatcgcaa ggtaaacgtt tgcctttaca caccttacgc acaacaaaag
    17401 tgttgcggta cgccggaaaa acgacacgga gcgtcaggaa ggggtcaatg gccagtacgc
    17461 aaatagtgta agcgtggaat tttggccgat cttgacgaaa aggggaagtg gtaaaaacac
    17521 ttatcattat aaatgacggg agattttttc atcacagtgt gtaaaaatgc gtaatatcac
    17581 tttttgtgtg gtaataaaaa aggagaaaac ttgatgagca ccgaccttaa attttcactg
    17641 gtaacaacga ttatcgtcct cggtttgatc gtagccgtgg gtttgactgc cgcgctgcac
    17701 tgatttcctc tcgagggagc gcgttctccc tcgcatctcc ttcctgtttg ttagcaaaat
    17761 ggcaaaagtg ttttctcttt ttgttatttt catttttttg tgattgctgt catgctttcg
    17821 ccactttaat gaatatcgcg taatgacgcg aagaggtgga gataaggcat gaaaattaac
    17881 tatccgttgc tggcgctggc gattggcgcg tttggtatcg ggacaacgga gttctcgcca
    17941 atgggcttgt tgcccgtcat tgcgcgcggt gtggatgtct cgattcccgc tgccggaatg
    18001 ttaatcagtg cctatgcagt tggcgtaatg gtgggcgcgc cgctgatgac gcttctactt
    18061 tctcatcgtg cccgccgcag tgcgttgatt ttcctgatgg caattttcac gctaggcaac
    18121 gttctttccg ccatcgcgcc ggattatatg accctgatgc tttcacgtat tttgaccagc
    18181 ctgaatcacg gagcattttt tggtttgggt tcagtcgtgg ccgcaagcgt ggtgccaaaa
    18241 cataaacagg ccagcgcagt tgccactatg tttatggggt taaccctggc aaatatcggt
    18301 ggcgtgccgg cggcgacctg gttgggtgaa accatcggct ggcggatgtc atttctggca
    18361 acggcggggc tgggagtgat ttcaatggta agtctgttct tctcattacc taaaggtggt
    18421 acaggggcac gacctgaagt gaaaaaagag ctggcggtat taatgcgtcc gcaggtgctg
    18481 tctgcattgc tgacgacggt actgggagct ggtgcaatgt ttaccctcta cacctatatc
    18541 tctccggtac tgcaaagtat tacccacgca acaccggtgt tcgtcacggc aatgctggtg
    18601 ctgattggtg tcggattctc tatcggtaac tatctcggcg gcaaactggc agatcgttca
    18661 gttaacggca cgttgaaagg ctttttgttg ctgctgatgg tgattatgct ggcaatcccg
    18721 ttcctggccc gcaatgagtt cggcgcagct attagcatgg tggtgtgggg cgcagcaacc
    18781 tttgcggttg tacctccgtt acagatgcgc gtgatgcatg tcgccagtga agcgccaggt
    18841 ctgtcttcat cagtcaatat tggtgccttt aatcttggaa atgcgctggg agcagctgct
    18901 ggtggtgcgg taatttccgc tgggctggga tacagctttg tgccggtgac gggggcgatt
    18961 gtcgcgggac tggcattatt gctggtgttt atgtcagcca gaaaacaacc tgaaacagtt
    19021 tgcgttgcta acagctaatt aaacaaaaac gcagaaggat aaccttctgc gttacagttg
    19081 ctgacaacgt gcgatttatc ttgccgaatg cgctgttaac gcctcatccg cgttggcatg
    19141 gtaagcgaag cgcatcaggc aatgctgcat ttgccatcag ttattatgca gcgagatttt
    19201 tcgctacgaa ttcccagttc accagcgccc agaagtgctc cagatagcca ggacgtgcat
    19261 tgcgatagtc gatgtaataa gcgtgttccc agacatcaac ggtcagcagc ggagtcgcat
    19321 cggtggtcag cggagtaccc gcgttagagg ttgaaacgat agccagtttg ccatcgctgt
    19381 ttttcaccag ccaggtccag ccagaaccaa agtttttgat cgctgcatca gtaaactgcg
    19441 ctttgaaatc ggcaaagctg ccaaaagatg cggcgatagc ttcagcgact tttccagtcg
    19501 gttcgccacc ggcgttcggt gccaggcagt tccagtagaa agtatggttc cagacctgag
    19561 ctgcgttgtt gaatacgcca ccttcagagc tgcgaataat ctcttccagt gatttacctt
    19621 caaacgcggt acctttaatc aggttgttca ggttagtgac ataagtctga tgatgcttgc
    19681 cgtagtgata ctcgatagtt tccgcagaaa tgtgcggtgc cagagcatct ttagcatatg
    19741 gtagtgcagg taattcgaat gacattgcta ctctccttta ttattaattt gcatacgtac
    19801 aatagcctta ttgtgcgtat ggatagggta gcaaattaaa aggtgagaca aaagatgaac
    19861 ttaccctgtt gccgtaacaa cagggtaaag ttttagcgaa gtgtttttgg ggtcattacc
    19921 cgacgagcgc caacatagtg gcgctgccag tagtcttcac tgagagaagt gatttgaatt
    19981 tcctgacctg tgcgcggtga ctgaataaat ttgccgttgc cgacatacac gccgacatga
    20041 tcggctgtac cgcgtccctg agtacggaaa aagaccaggt cgccgttttt cagctcacta
    20101 cgttcgatag gtcctgcatc acgcaggtga tacatttcat tcgccgtacg cggaatacga
    20161 attttcacca aatctttata agcgtaataa accaggccgc tgcaatcaaa accggtacgc
    20221 ggtgagctgc cgccccaacg atatggctta ccaatttgct gcatcagttt attcattgcc
    20281 actttcgtgg ctttttgcac cttcgctttg tgcgcgtcgg caagggttcc tgacgcggca
    20341 tttttgactt tcacacaatg cgatttatag cccttacgtt tggtacactt ttcggtaaag
    20401 ctggctgagg ccgttttatt gacggcgtgt ttgctgcgag ttttggcgtt tttagaagcg
    20461 gtaatggaag attttttaac agaagaagcg gttttgcttt tggtcatact tttactggcg
    20521 gcttttttcg ctgttttctg gctggctttc tgggtctttt tggtggttgc cgtgcttttc
    20581 tttttatctg cttttttggt gatatgagtg gtagcagaac tctccctggc ttgctttgaa
    20641 gcatgggcca taggcgttaa aggcagggtg gtaaaaagta aagcacagag cgtgatcgaa
    20701 atacggttta tccgcgccac tgagcgtccc tctgttaagg caggtctatc atgcctgcgt
    20761 ctgatttaca aaacttgatg attctaatgc atagaagaag agaaagttaa tagcttttat
    20821 tgttaaaaaa tacgtttcgt tggcgagcgc aaaaaaatgc agcagtgaaa gctaaaaatg
    20881 gttataaata cgccagtttg ccgcgcatca gagaagaaac gaatatttga aatgcaacaa
    20941 atagagcaaa gggataaaat agcaaaagcg tgatgaaaat gttattttcc gatgccgcac
    21001 tgacccgcta caatgtcaga ttactgccgt aaagaagttt gaggaagcaa gacaatgagc
    21061 accactatcg aaaaaatcca acgccagatt gctgaaaacc cgatcctgct gtacatgaaa
    21121 ggttcaccga aactgccgag ctgcggtttc tctgcccagg cagtccaggc gcttgccgca
    21181 tgtggcgaac gttttgccta tgttgatatt ctgcagaatc cggacattcg tgcggaactg
    21241 ccgaaatatg ctaactggcc gaccttcccg caactgtggg ttgacggcga gctggtcggc
    21301 ggttgtgata tcgtgatcga aatgtatcag cgtggcgaac tgcagcagct gatcaaagaa
    21361 actgccgcta aatacaagtc tgaagagccg gacgcggaat aatccgtcct gcaatactga
    21421 cggatgcgaa cgggcatccg tcagtccttt atccggtgcg tcgtaaaacc tcgtccttca
    21481 gggcggggat ataaggcgcg gttttccacc tgaccaggtg tttgctgttg ttcaatgtac
    21541 tggcggatga cggatattgg cgcaccttcg cagctactgg caaaatagcc aggactccac
    21601 aaaacgcctt tgtagtaata cctgactgca atatctggtc gatctcgtcg cagtaaccta
    21661 ttcgatatgc ctttgaggct gtttaccaga ctggatatcg ccagtttggg aggatagtta
    21721 attaacaaat ggacgtgatc tggttcgcca tccatttcaa ccagttcagc tgcaaaatca
    21781 gcacatacat ttgaaaagta agtgcgtagt ttttctgtcg tgtcatggtc aaaaatctgg
    21841 cgtcggtatt tggtgacaaa gaccaggtga acatgcatca ggaaaacaca atgccttcca
    21901 cgccggatat cggtttcttt tttcacagac caaagtatca tttgacctgt gaaacgatta
    21961 caggcattta aattccagtt aagacccggt ggtcaacagg agcgtgaaat gaggcgcttc
    22021 gcaggcgcat gtcgtttcgt tttcaatcgt gctctggcgc ttcagaatga gaatcatgag
    22081 gccggtaata aatacatccc ttacgggaaa atggcttcct ggctggttga gtggaaaaac
    22141 gccactgaaa cgcaatggct taaagattcg ccctcacagc cattgcaaca gtcactgaaa
    22201 gaccttgagc gggcttacaa aaacttcttc cagaagcggg ctgcttttcc ccgattcaaa
    22261 aagcggggac agaatgctgc attccgctac ccgcaggacg ttaagctcga tcaggaaaac
    22321 agccgtattt ttctgccgaa acttggctgg atgcgctacc ggaatagccg tcaggtcacg
    22381 ggagttgtga aaaatgtcac tgtcagccag tcctgcggta cgtggtacat cagtattcag
    22441 acagaaagtg aagtatcaac tccggttcac ccttcagcat caatgatcgg gctggatgct
    22501 ggcgtggcta aactcgccac gctgtcagat ggcacagtct ttgagcctgt aaacagtttt
    22561 caaaaaaacc agaagacgct ggcgagactt cagcgacagt taagccg
//
LOCUS       DAJVPP010000064        22453 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVPP010000064 DAJVPP010000000
VERSION     DAJVPP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.064"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            337..2534
                     /gene="speF"
                     /locus_tag="OQM34_002516"
                     /pseudo
     CDS             337..2534
                     /gene="speF"
                     /locus_tag="OQM34_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2531..3850
                     /gene="potE"
                     /locus_tag="OQM34_002517"
     CDS             2531..3850
                     /gene="potE"
                     /locus_tag="OQM34_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS1452916.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3906..5546)
                     /gene="pgm"
                     /locus_tag="OQM34_002518"
     CDS             complement(3906..5546)
                     /gene="pgm"
                     /locus_tag="OQM34_002518"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1452917.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5572..6117)
                     /gene="seqA"
                     /locus_tag="OQM34_002519"
     CDS             complement(5572..6117)
                     /gene="seqA"
                     /locus_tag="OQM34_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1452918.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6302..7066
                     /gene="ybfF"
                     /locus_tag="OQM34_002520"
     CDS             6302..7066
                     /gene="ybfF"
                     /locus_tag="OQM34_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1452919.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7206..7499
                     /gene="ybfE"
                     /locus_tag="OQM34_002521"
     CDS             7206..7499
                     /gene="ybfE"
                     /locus_tag="OQM34_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1452920.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7639..8169
                     /gene="fldA"
                     /locus_tag="OQM34_002522"
     CDS             7639..8169
                     /gene="fldA"
                     /locus_tag="OQM34_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1452921.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8379..8465
                     /gene="uof"
                     /locus_tag="OQM34_002523"
     CDS             8379..8465
                     /gene="uof"
                     /locus_tag="OQM34_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1452922.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8458..8904
                     /gene="fur"
                     /locus_tag="OQM34_002524"
     CDS             8458..8904
                     /gene="fur"
                     /locus_tag="OQM34_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1452923.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8988..9314)
                     /locus_tag="OQM34_002525"
     CDS             complement(8988..9314)
                     /locus_tag="OQM34_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452924.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9351..10757)
                     /gene="chiP"
                     /locus_tag="OQM34_002526"
     CDS             complement(9351..10757)
                     /gene="chiP"
                     /locus_tag="OQM34_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCS1452925.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11336..13000)
                     /gene="glnS"
                     /locus_tag="OQM34_002527"
     CDS             complement(11336..13000)
                     /gene="glnS"
                     /locus_tag="OQM34_002527"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1452926.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWLDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13203..15149)
                     /gene="nagE"
                     /locus_tag="OQM34_002528"
     CDS             complement(13203..15149)
                     /gene="nagE"
                     /locus_tag="OQM34_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1452927.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVIINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            15482..16282
                     /gene="nagB"
                     /locus_tag="OQM34_002529"
     CDS             15482..16282
                     /gene="nagB"
                     /locus_tag="OQM34_002529"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1452928.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16342..17490
                     /gene="nagA"
                     /locus_tag="OQM34_002530"
     CDS             16342..17490
                     /gene="nagA"
                     /locus_tag="OQM34_002530"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1452929.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17499..18719
                     /gene="nagC"
                     /locus_tag="OQM34_002531"
                     /gene_synonym="nagR"
     CDS             17499..18719
                     /gene="nagC"
                     /locus_tag="OQM34_002531"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1452930.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18767..19519
                     /gene="nagD"
                     /locus_tag="OQM34_002532"
     CDS             18767..19519
                     /gene="nagD"
                     /locus_tag="OQM34_002532"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCS1452931.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            19916..21580
                     /gene="asnB"
                     /locus_tag="OQM34_002533"
     CDS             19916..21580
                     /gene="asnB"
                     /locus_tag="OQM34_002533"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1452932.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21959..22035
                     /locus_tag="OQM34_002534"
     tRNA            21959..22035
                     /locus_tag="OQM34_002534"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21993..21995,aa:Met,seq:cat)
     gene            22044..22128
                     /locus_tag="OQM34_002535"
     tRNA            22044..22128
                     /locus_tag="OQM34_002535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22078..22080,aa:Leu,seq:tag)
     gene            22152..22226
                     /locus_tag="OQM34_002536"
     tRNA            22152..22226
                     /locus_tag="OQM34_002536"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22184..22186,aa:Gln,seq:ttg)
     gene            22261..22335
                     /locus_tag="OQM34_002537"
     tRNA            22261..22335
                     /locus_tag="OQM34_002537"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22293..22295,aa:Gln,seq:ttg)
     gene            22351..22427
                     /locus_tag="OQM34_002538"
     tRNA            22351..22427
                     /locus_tag="OQM34_002538"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22385..22387,aa:Met,seq:cat)
BASE COUNT         5517 a         5664 c         5652 g         5620 t
ORIGIN      
        1 actttcattt ctttaatgcc cgctagtctt ctgactaaag ggcaccccaa cgtacaggtc
       61 tccctgactt taagcattac aggttaatac ctgtattcct cggtgctcat atactgctaa
      121 cccttttaaa ctctaaataa ttcgagtcgc agcacttgca acttgaggta tgacgagtat
      181 agccagttac cgggctggtc tgggttattg catctgcaaa aagcaaacta ctgatttatt
      241 tatcagcggt ggagctttgc tttttttccg acgtgatcga tttctccttt gagaaattga
      301 ggacctgcta ttacctgaaa taaagagatg aataaaatgt cagaattaaa aattgccgtt
      361 agccgttctt gcccggattg tttttccact catcgtgcat gcgtgaatat tgacgaaagt
      421 aattatattg acgttgccgc cattatttta tcagtcaatg atgttgaacg tggaaaactc
      481 gataaaatag acgctactgg ctatggcatt cctgttttta ttgcaacgga aaatgaagaa
      541 cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg cgaatcacgg
      601 aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac gcaactgcgc
      661 ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc ttttgactgc
      721 ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca gttcgtggag
      781 tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc aatgggcgat
      841 ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa agtgtttaat
      901 gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt ggttttaaac
      961 gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa atctaaccac
     1021 cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc acgtaacccg
     1081 tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct gcgtgagctg
     1141 atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct cgctgtgatt
     1201 cagttaggca cctacgacga tacgatttat aacgcccgcc aggtggtgga taaaattggt
     1261 catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca atttatcccg
     1321 atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc gggtattcta
     1381 gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca aatccataaa
     1441 aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat gaacaacgcc
     1501 tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact ggatatcaac
     1561 gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt ggtaaatggc
     1621 attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt cgtacctgaa
     1681 ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt tgatctgcgc
     1741 ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc agagaatcaa
     1801 tactttgtcg atccttgcaa actgttgctg acaaccccag gtattgatgc acgtaacggc
     1861 gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg cgaaaatggc
     1921 gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc ggcagaagat
     1981 atggccaaac ttcagcaact tgtgccctgc tggtacgctt cgaaaaactg cttgagtccg
     2041 acgcgccatt agcagaagtg ctgccttcca tctacaaaca gcatgaagag cgctacgccg
     2101 gttataccct gcgtcagttg tgccaggaaa tgcatgattt gtatgcccgc cacaacgtga
     2161 aacaactgca aaaagagatg ttccgcaaag cgcacttccc acacgtcagc atgaatccgc
     2221 aagaagccaa ctacgcctat ttacgcggtg aagtggagct ggttcgtctg ccggatgccg
     2281 aaggtcgtat cgctgccgaa ggggcgcttc cttatcctcc gggtgtgctg tgtgttgttc
     2341 cgggtgaaat ctggggtggt gctgttttgc gttacttcag cgctctggaa gaagggatca
     2401 acctgctgcc aggttttgca ccggagctgc agggtgtcta tatcgaagaa catgatggtc
     2461 gtaagcaagt ttggtgctat gtcatcaagc ctcgtgatgc gcaaagcacc ctgttgaaag
     2521 gggaaaaatt atgagtcagg ctaaatcgaa caaaatgggc gtcgttcagt taaccatact
     2581 gacgatggtc aacatgatgg gctccggtat catcatgctg ccgacaaagc ttgccgaagt
     2641 tgggacaatt tcaattatct cctggctggt gacagccgta ggctcaatgg cactggcatg
     2701 ggcattcgca aaatgcggta tgttcagccg taaatcaggt ggtatgggcg gttatgccga
     2761 gtatgctttc ggtaaatccg gtaactttat tgcgaactat acctacggcg tctcactgct
     2821 gattgctaac gtcgcgattg ctatttcggc ggttggttac ggcaccgaac tgctcggcgc
     2881 gagtttgtcg ccagtgcaga ttggtcttgc gaccatcggg gtgctgtgga tttgtaccgt
     2941 ggctaacttt ggtggtgcgc gcattaccgg gcaaatcagt agcattaccg tgtggggggt
     3001 cattattccg gtggtaggcc tgtgcattat cggctggttc tggtttagcc cgacgcttta
     3061 cgttgattcc tggaatccgc atcatgcacc gttcttcagt gcagtaggtt cttccatcgc
     3121 tatgacgctg tgggcttttc ttggtctgga gtctgcgtgt gcgaatactg atgtagtgga
     3181 aaacccggaa cgtaatgtgc caatcgcggt actcggcggt acgttaggtg cggcggtgat
     3241 ttatatcgtc tccaccaacg tgattgcagg gattgtgcct aatatggagc tggcaaattc
     3301 aacggcacca tttggcctgg ccttcgcaca gatgtttaca ccagaagtgg gtaaagtcat
     3361 tatggcgctg atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt tcaccattgc
     3421 ccaggtgttt aaatcttcat ctgatgaagg ctacttccct aaaattttct cccgtgtaac
     3481 caaagtggat gcaccggtgc agggaatgtt gaccattgtg attatccaga gcgggttggc
     3541 gctgatgacg attagcccgt cgctaaacag tcagttcaac gtgctggtta acctggccgt
     3601 ggtgaccaat atcattccgt atattctgtc aatggcggca ttagtcatta ttcagaaggt
     3661 cgctaatgtg cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat
     3721 gtatagcttt tatgcgctgt actcatccgg ggaagaagcc atgctgtacg gttccatcgt
     3781 gaccttcctt ggttggacac tgtatggtct ggtatcacca cgctttgaac tgaaaaataa
     3841 acacggttaa aaaagcaaag taaaaaaggg cgatcttgcg accgctcttt ttttattaaa
     3901 tgtgtttacg cgtttttcag aacttcgcta acaatctcaa ccgcttcttt ctcaatctgc
     3961 ttgcgatgtt cttcaccgag gaagctttcg cagtagatct tatatgcgtc ttccgtgcct
     4021 gacggacgcg cggcgaacca gccgttgtca gtcatcactt tcagaccgcc aatagaagca
     4081 ccgttgcccg gagcagcagt caggcgcgcg gtgatcgggt cacctgccag ggtgctggcg
     4141 ctcaccattt ccggagacag cttagacagc gccgcttttt gtgcggaagt cgcagctgcc
     4201 tgcaaacggt tgtagctcgg cgcaccaaag cgttttgcca gttcgttgta gtgttcctgc
     4261 gggttcttac cggtgacagc ggtgatttcc gccgccagca gacacataat gatgccgtct
     4321 ttgtcggtgg accacggcgt gccgtcgaaa cgcaggaagg aagcccctgc gctctcttcg
     4381 ccgccaaagc cgaagctgcc gtcgaacaga ccatcaacaa accatttgaa acctaccggt
     4441 acttctacca gcttacggcc caagtcattg accacacggt cgatcatcgc agaagaaacc
     4501 agcgttttac cgacagcaac atctttgccc cactgcggac gatgctggaa caggtagttg
     4561 atcgccaccg ccaggtagtg gttcggattc atcaaacctg ccggagtgac gataccgtga
     4621 cggtcataat ccgggtcgtt agcaaacgcc agatcgaact tatcacgcag tgccagcaga
     4681 cccgccatcg cacactcgga ggagcagtcc atacggatcg caccgtcttt atcgaggtgc
     4741 ataaagcgga aggtttgatc gacctgatcg ttaacgatgg tcaggttgag gttgtaatac
     4801 tcgccaatgc gcttccagta ttcgataccg gaaccgccca acggatcaac gcccagcgtc
     4861 aggcccgctt tctgaatcgc cgccatatca acgatatcgg ccagcccttc cacgaacggc
     4921 tgcaccagat cctgctcttt cacatagccg gatgccatcg cttcgtcgag ggagatacgc
     4981 ttcacgcctt tcaggccatc ggccatcagt gcgttggccc tgtcttccac cactttggtg
     5041 acgttagtat cagccgggcc accatttggc ggattgtact tgataccacc atcttccggc
     5101 gggttatggg acggtgtaat cacgataccg tctgccagcg ggccaccttt tttattgtga
     5161 accaggatgg cattggaaac ggcaggcgtc ggagtgaagc cattgttttc ctgcacaatg
     5221 acatcaacgc cgttcgctgc cagcacttcc agtacggaaa tgaacgcagg ctcggacagg
     5281 gcgtgagtat ctttacccac atagcaaggg ccagtgatgc cgtttttcgc acgttcttca
     5341 gcaattgcct gagcgatcgc cagaatgtgc ggctcgttaa agctgtggcg cgctgcactg
     5401 ccacggtgac cggaagtacc gaatttcacc gcgtgctccg cattccctgc ttctggtttc
     5461 agtacataat attgcgccgt cagttgggcg acgttaatca aatcactctg ttgtgcaggt
     5521 tggcctgcac gattgtggat tgccattgct ttgtcctttg tctgcaacgt tttagatagt
     5581 tccgcaaacc ttctcaatca attccgccgg gaattgcatc gactgcatga tgtgttcgat
     5641 catgctgcat ttacggccgg tgttggtgtt ggtgatcacc caatacggcg tgcctggcac
     5701 atgtttcggc ttggtctgat taccattttt cagcagcgtt tgttcatctg ccgcaaagta
     5761 cacgcgtgta cgaccgtgca acgattccgt tgcttcggca aacgcctggg cgtcaagaga
     5821 atatagtgta gacaacagca gcataaagcg attgaccgct cgcttttgct ctgcgtattc
     5881 gtccgaaagc agaagttcac gcattgcgcg aaccttgtct ttaatcgttt tgaccggctt
     5941 cgcttcgacg atagcaggtg acgcaacgcg aacctctttc gtcaccggag cagcaggctg
     6001 tgatgcggcg gaaaatttca acatacgccg taaaatgtcg gatgcgctct cgccgatatg
     6061 cttagtgtgg ctggcaatat agctgtagag ttcatcatca acttcaatcg ttttcatctt
     6121 aatccagtgc agtgtcttag ctgaatacaa gtcgccaaga gtatatgaat aaatcccggc
     6181 agcggatagc gtcaagcctg gcttgcagaa aaactcggaa taaaccttat ttgttgcagc
     6241 cctgtggcac aatggtcaac ataataccct aacccgacaa cgcgaaaaaa agaactttgc
     6301 catgaaatta aatatccgcg cgcaaactgc acaaaaccag cacaataatt ctcccatcgt
     6361 tcttgtccat ggtctgtttg gtagcctcga caaccttggc gtactggctc gcgatctggt
     6421 aaacgatcac aatatcatcc aggttgatat gcgtaaccac ggtctttcac cgagagagcc
     6481 ggtaatgaat tacccggcga tggcgcagga tcttgttgat accctggatg cactgcagat
     6541 cgacaaagca acatttatcg gtcactccat gggcggtaaa gcggtaatgg cacttactgc
     6601 actggccccc gatcgcatcg ataaactggt ggcgatcgat atcgcaccgg tcgactatca
     6661 cgtacgccgt catgatgaga ttttcgcagc tatcaacgcg gtcagtgaat cggacgcaca
     6721 aactcgccag caagcagcag caataatgcg ccagcatctt aatgaagaag gggtgattca
     6781 gtttctgttg aaatcttttg ttgacgggga gtggcgcttt aacgtgccgg tattgtggga
     6841 tcagtatccg catattgtgg gttgggagaa aatcccggca tgggatcacc ctgccctgtt
     6901 tatccctggc ggcaattctc cgtatgttag cgagcagtac cgtgatgatt tactggcaca
     6961 atttccacag gcacgggcgc atgtgattgc aggcgcgggt cactgggtcc atgctgaaaa
     7021 accggatgcg gtattacgcg ctatccgtcg ctatctcaat gattaacgca actgattaaa
     7081 aacccagcgt ccgcgtcgct ttccgcgcgt gggcgttggc gtgactcatc gcgctgatgt
     7141 attatggcgc gctatccatc cgggcagagg cctggctgat tgtttccccc gaagtcacca
     7201 agatcatggc caaagaacaa acggaccgta cgacattaga tctgttcgcg cacgagcgtc
     7261 gaccgggacg accgaaaact aatccgcttt cgcgcgatga acagctgcgt attaataaac
     7321 gcaaccagct aaaacgcgac aaagtacgtg gccttaagcg tgtcgaactg aagctgaacg
     7381 cggaagctgt cgaggcgctg aacgagctgg cggaatcgcg taatatgagc cgtagcgaac
     7441 tgatcgaaga gatgttaatg cagcaactgg cggcgctgcg tagtcagggc atcgtttaaa
     7501 tttccacttt catgtagcac agtgtgcagt cctgctcgtt tgctgattcc gcaagactgc
     7561 ctgttctgct atgattgcct ttatccgtgg gcaattttcc acccccattt caataagttt
     7621 caagaggtta tttcactcat ggctatcact ggcatctttt tcggcagcga caccggtaat
     7681 accgaaaata tcgcaaaaat gattcaaaaa cagcttggta aagacgttgc cgatgtccat
     7741 gacattgcaa aaagcagcaa agaagatctg gaagcttacg acattctgct gctgggcatc
     7801 ccaacctggt attacggcga agcgcagtgt gactgggatg acttcttccc gactctcgaa
     7861 gagattgatt tcaatggtaa actggttgcg ctgtttggtt gtggtgacca ggaagattac
     7921 gccgaatatt tctgcgacgc attgggcacc atccgcgaca tcattgaacc gcgcggtgca
     7981 accatcgttg gtcactggcc aactgcgggc tatcatttcg aagcatcaaa aggtctggca
     8041 gatgacgacc actttgtcgg tctggctatc gacgaagacc gtcagccgga actgaccgct
     8101 gaacgtgtag aaaaatgggt taaacagatt tctgaagagt tgcatctcga cgaaattctt
     8161 aatgcctgat gtgatgcggc gtagactcat gtctacgccg tattaataga taatgccaat
     8221 caaaataatt gctacaaatt tgtaactttt gctgttgtac ctgtacaatg tcccagtgtt
     8281 caagtggcct tgccgttgta aatgtaagct gtgccacgtt tttattaaca atatttgcca
     8341 gggacttgtg gttttcattt aggcgtggca attctataat gatacgcatt atctcaagag
     8401 caaattctgt cacttcttct aatgaagtga accgcttagt aacaggacag attccgcatg
     8461 actgataaca ataccgccct aaagaaagct ggcctgaaag taacgcttcc tcgtttaaaa
     8521 atcctggaag ttcttcagga gccggacaac catcacgtca gtgcggaaga tttatacaaa
     8581 cgtctgatcg atatgggtga agaaattggt ctggctacgg tatatcgcgt actgaaccag
     8641 tttgacgacg ctggtatcgt cacccgccac aattttgaag gcggtaaatc cgtatttgaa
     8701 ctgacacagc aacatcacca cgatcacctg atttgcctcg actgcggcaa ggttatcgaa
     8761 tttagtgatg attccatcga agcgcgtcag cgtgaaattg ccgcaaaaca tggcattcgc
     8821 ctgactaacc acagtctcta tctttacggt cactgtgccg aaggcgattg ccgcgaagat
     8881 gagcacgcgc acgaaggcaa ataagccagc ctgaacgaga aaagccaacc tgcgggttgg
     8941 cttttttata caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta
     9001 ctgcgtattt cctgccagac cttatcacaa tcggctttca ccgcctgatc atttccggtt
     9061 tgcgttgcac gcagacactg ctgataatcc agtacgcgta cactttcctg gtcggcaaac
     9121 tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt
     9181 tctggcgaac cttgcgcggt gttgatacag gcgctgtatg cctctttcag acggctgtct
     9241 tcctgtggtg cggttgattg cgcacaagct accagccccg atgccatcat ggcgatgaga
     9301 atcagttttt tcattgaatg cggcaggcac aaaacctgcc gcgtcgggca tcagaagatg
     9361 gtgaatggtg cgattaccat aaatttcacg tcacgctcat cctggaagat gttaccgtaa
     9421 ccgccgcccc agcttgggat gtcggagtgg ttgtcgtatt cggtgaagtg cagtttgaac
     9481 atcgtgcctt tggcgcgacc gtcctgaatg gtgtagaccg catccaggct gtaggcagac
     9541 tcttcaatag tccggttttt gtcgtagtac gcatccggat tgctctgcca ggtcgcaggt
     9601 ttagcatccc atgcgtaaac gtaggaagcg ccgatggcga agcctggaag attccagttt
     9661 ttcaggtcat acatcgcact gaagaagacc gctttttcgc cgttagcgtt gaagtcggaa
     9721 cggttatccc accagatatc caggcggccg ttggaggaag cgtaggttgg ggtcatacgt
     9781 tgcaggaagt atccctgctg actgtcagcc ttaatccagg tgccttcgag gcgcaaatcc
     9841 actacgtcag ccgcccggta accaaaggtc aacgcttgca gccaggcggt gccgtcataa
     9901 agatcgttga cgctgcgatc gtcaacttta tcgcgggtac catagaactg gtagctggtg
     9961 gttaacgggc taccggcgat atcaaatttg tagctggctt tggcaaaata ttgatcgata
    10021 tacccttccg cctgaccaaa tgccgcttcc agtacgaagt tatttttgaa gtcgtatttc
    10081 gcaccgatgg agtgcagata atcaactttg gtggttttat cgttctgata aaactcatcc
    10141 atttccagat gccacggcgc tttgtattcg ttggtccaca tgtaggagaa actcaaggca
    10201 ccagcatcgc cgtaatcaaa attcgccccg gcttccgccc cctgataagt acccggcata
    10261 aagctccagt gcggcgctaa cagcgtttga ccggttggct gaatgtaacc tgccctcgcc
    10321 caaaccggac cgtatttaaa tttggccgca gctttataca ggcttatacc gcttttgtcg
    10381 ccggaccagt cttcgtcata ggctttatta ctttttgaaa acgcgatttc gttcgggtgg
    10441 gagctgtcgc cgttttccgc catttcaatc gccgtaaacg cagcaatatc aaggccgaac
    10501 atatcagcag cataaccgga ctgaaaatcg aggttggcat tccaggtgga gtgagaaagg
    10561 ttggttttgt atttgtcgcc gtcggtaaca tctttacggt cgcgttcacg ctgccagtaa
    10621 tagataccgc cggttaaggt tgaatcgtcg atgaatcctt cggcgcgagc ctccggcatt
    10681 gccataaagc ccgacattgc tgtaacaccg gcaatagcca gcgccagcgt actacgtttg
    10741 ccactaaacg tacgcatggg ttaatcctct ttgacgtata aattgctgca ccaaaggtga
    10801 acagcgaaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc tgactacagg
    10861 aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga atgtaagaac
    10921 atgagcaacc gcacatattt tgatgatttt tgttacaagg cttatgtttg cggttgaaag
    10981 gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aagcggttac attttattta
    11041 attatgtttt gtgattttaa atttatttta tggataaatt gcgaagcgga attaattcgc
    11101 gaagaaaagc agaaaaaacg ccgcagagcg cggcgtttgg gattgtttgc tatttcttgc
    11161 cggatgcggc gtgaacgcct tatccggcct acaaaagcac gcaaattcaa tatattgcag
    11221 agatcatgta ggcctgataa gcgaagcgca tcaggcaatt tagcgtttga aatttattgt
    11281 ctatttattg ccggatgcga cgtaaatgcc tcacccggct tggtagaact taaaattact
    11341 cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc ggtttttcgg
    11401 ccgtggaatg gcggctgtcg aggcagaagt aaccttcacg ctcaaactgg aatgctttac
    11461 ccgcaaccgc atctttcagc gacggttcag caaagccctg tttgatcacc agcgattccg
    11521 ggttaatcac cgacaggaaa tcatccgcag cacctgggtt aggcacgctg aacagacgat
    11581 catacaaacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga ataacacctt
    11641 tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa gtacagaaga
    11701 tggtggtgat attaccttcg gcatctttct ccacgcgttc agccttaatg acgtaagcat
    11761 tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta gcttcttcgc
    11821 ggaaatcggc gcgatcgagc caaatttcac cgctaaacgg cacctgacgg ctccccattt
    11881 ccggtttgtt cggatggttc ggcatggtga ccatttcgcc ttcgccctga tagttttcga
    11941 taaccagttt caccggatcg ataaccgcca ttgcgcgcgg cgcattttcg ttgagatctt
    12001 cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg gtcacgccga
    12061 tgcgtttgca gaactcacga atagaagccg cagtgtaacc acgacgacgc agaccggaaa
    12121 tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc agcaggttca
    12181 gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg tactggcgcg
    12241 gatgaacagg aatggtgatg ttatccagta cccagtcgta cagacgacgg ttgtcctgga
    12301 actcaagcgt acacagagag tgcgtaatac cttccagcgc atcgctgatg caatgggtga
    12361 agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca gcaaatttaa
    12421 tgcggtacag caccggatcg cgcattacga taaatggcga cgccatgtcg attttcgcac
    12481 gcaggcaggc tttaccttct tcaaaaccac cggcacgcat tttttcgaac agcgccaagt
    12541 tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggctgcgtc agggtaccgc
    12601 ggtattcgcg gatctgttcc ggtgtcagtt catcaacgta cgccaggcct ttattgatca
    12661 gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg acgttaccag
    12721 accagtgaaa acctaaccac tctacgtcgt ttttgatcga atcgacatac tcgatatctt
    12781 cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag tcctgggcga
    12841 tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca ttcggctccg
    12901 gcgggaaacg ggtgtgtact gtgatgtgct taccactggc cagatcttca tcgatgatct
    12961 gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc tcaaagcgta
    13021 aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg ttagtttttt
    13081 aaaattgata atctaacaag gattatgggg atgattgatg aaaaaaaaag cggcagagaa
    13141 gatctccacc gcttgtcatt gttggatgcg acgctgccgt gtcgcatcag gcataaagca
    13201 gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc ctgagcttta
    13261 atgagcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat catcgagcgg
    13321 gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc gcttacctgc
    13381 gcaccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt gtcgataccc
    13441 atatggacga cgatctccgc gcctttttcg gtttccaggc agaacgcgtg gttggtgttg
    13501 aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt cggtttcacc
    13561 gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg atccagtgcc
    13621 acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt tggtacagcc
    13681 tgcggttttg ctacaggcgc ggcagttgcc ggagttgctt cagctgacgc agcggctacc
    13741 ggaccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt cgcgccaaca
    13801 atcacctgaa tagtctgttt gttcagtttc actacaccag aagcacccag acgtttacac
    13861 atcgtatcgt tgacgcgggc agagtcagcc acggtcaggc gcagacgggt aatacaggca
    13921 tcaatcgctt tcaggttgtc agtaccgcca accgcagcaa tatagttggt tgccagttga
    13981 gtcagacctt cttcagtatt gctgttggct tcttcagtaa cgatctcgtc ttctttatct
    14041 tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac cacgaagtag
    14101 atagcgaaga agataacgcc catcaccagc agcatccaga cgttctggct ggcggcaggc
    14161 aggttataca tcaacgcgta gtcgatagca cccgcagaga aagagaagcc ggcatggata
    14221 cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag gaggtacagc
    14281 agcggcgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt caggaacgca
    14341 gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt cggtgctgcg
    14401 aagtacatcg ccagcgccgc acccggcaga ccgaacatca tgatcgggaa gaagccggac
    14461 atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc gtggaaaacc
    14521 gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt cagcacctga
    14581 tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga acccagcgcg
    14641 cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg cggccagacg
    14701 taaccaaaaa tggccgccag caccagacag aagaagccgg tggcgatcgg cacaaagcgt
    14761 ttgccgccga agaagctcag gaagtccggc agtttaatat cggaccaacg gttataggct
    14821 gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat ttctgggttg
    14881 atgatcacca tcgctttggt taacacaaag taacctaccg cacccgccag cgccgccgca
    14941 cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa tgcgaggtta
    15001 tcaaaaattg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc tggctgaccg
    15061 aatcgcagca acagcgccgc caccggcagc accgcgatag ggagctgtaa cgccctaccg
    15121 agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc ctatttggct
    15181 cgtttaaagc cgtattttta ttttgctgca aattttactg ccgatgttct gtaatcagat
    15241 tgttagatca tctgctacag agtgtgtgaa aatttaattc gcatcgcaaa ttaaacgcgt
    15301 gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac tggctaaacc
    15361 agaaaactta ttttatcatt caaaaaatca agtcggattg acgcctgtct gcgcaaatcc
    15421 aggttacgct taaagatgtc taatccgcca acggcttaca ttttacttat tgaggtgaat
    15481 aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg ctcgccatat
    15541 cgtcaatcgt atcaatgcgt tcaaaccaac tgccgatcgt ccgtttgtac tgggcctgcc
    15601 gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata aagcaggcca
    15661 ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga
    15721 gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg ttgatattcc
    15781 agcagaaaac atcaaccttc tcaacggcaa tgccccggat atcgatgccg agtgccgcca
    15841 gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg gtgtaggtaa
    15901 cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa
    15961 aaccctgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca
    16021 ggtgccaaaa tatgccctga ctgtcggtgt tggtacactg ctggatgccg aagaagtgat
    16081 gattatggtg ctgggtagcc agaaagcact ggcgctgcag gccgccgttg aaggttgcgt
    16141 gaaccatatg tggaccatca gctgtctgca actgcatccg aaagcgatca tggtgtgcga
    16201 tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg aattagaagc
    16261 agaaaatatc aaaggtctgt aattgttatc cctgccctgg ttccttgctc agggcaatat
    16321 tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt taccggccac
    16381 gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag cgtctgtccg
    16441 gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat tctctccccc
    16501 ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga caccgctgaa
    16561 gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc aggctgtact
    16621 aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt
    16681 atgcgtgagt acctggcaaa acatccgaat caggcgttag gtctgcatct ggaaggtccg
    16741 tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa gcctgatgcc
    16801 gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg
    16861 gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc
    16921 ggtcactcca acgcgacgtt gaaagaagca aaagccggtt tccgcgcggg gattaccttt
    16981 gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg cctggcgggc
    17041 gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct gcatgttgat
    17101 tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct ggttaccgac
    17161 gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa aacaatatac
    17221 taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc cttaaccatg
    17281 attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga agtgctgcgt
    17341 atggcgacgc tctatccggc gcgtgcgatt gacgttgaaa aacgtctcgg cacgctcgcc
    17401 gcaggtaaag tagccaacct gaccgcattc acacctgatt ttaaaatcac caagaccatc
    17461 gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc ggacaagctc
    17521 agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg
    17581 accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt gcccccgcca
    17641 gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa gttgatcagc
    17701 aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc aatttccacg
    17761 caatcggcgt acggcttggt cgtcatgacg ccaccatcac tctgtttgat ctcagcagca
    17821 aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg ctggaacatg
    17881 ccctgctgaa tgccattgct cagtttattg atagctacca gcgtaagctg cgcgagctaa
    17941 tcgccatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa attcattaca
    18001 tgccgcatat tcaggtagaa aactgggggc tggtagaagc actggaagaa cgttttaaag
    18061 tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac tacttcggtg
    18121 caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc ggggccggga
    18181 ttatctctaa cgggcgcatt tttattggcc gcaacggtaa cgtcggtgaa attggccata
    18241 ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc ctggaaacta
    18301 tcgccgccaa cgctgccatt gaacaacggg tgttgaatct gttaaagcag ggctaccaga
    18361 gccgtgtgcc actggacgac tgcaccatca aaactatctg caaagccgcg aacaaaggcg
    18421 atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggtaaa accatcgcca
    18481 ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc tggtgaaatc accgaagccg
    18541 ataaagtgct gctccctgct attgaaagct gcattaatac ccaagcgctg aaggcgtttc
    18601 gtactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc ggcgcttttg
    18661 cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg gaaaattaat
    18721 gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga ccattaaaaa
    18781 tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc
    18841 ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga ccaactatcc
    18901 ttcgcagact gggcaagatc tggcgaaccg ctttgccact gccggtgtcg atgtgccgga
    18961 tagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa
    19021 gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag ccggtttcac
    19081 tattaccgat gtgaaccctg atttcgtgat tgttggcgaa acacgttctt acaactggga
    19141 catgatgcat aaagcagcct atttcgtcgc taacggtgca cgctttatcg ccaccaaccc
    19201 ggacacccac gggcgcggtt tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa
    19261 aatctccggg cgcaaaccgt tctatgttgg taagcccagc ccgtggatca tccgcgcggc
    19321 attaaacaaa atgcaggcgc attcggaaga aacagtgatt gtcggcgata acctgcgtac
    19381 cgatattctg gcgggcttcc aggccggtct ggagacgatt ctggtgcttt ctggtgtttc
    19441 atcgctcgac gatatcgaca gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc
    19501 tgaaatcgac gttatctgaa taccacccac gtctgcggac gtggttggct ctgatgacaa
    19561 acgccaaact gcctgatgcg ctacgcttat caggcctacg cagctcctgc aatatattga
    19621 attttcatgc ttttgtaggc cggataaggc gttcacgccg catccggcat gaacaaatcg
    19681 cactttatca gcaatttggc ccccgtaaaa tataaccatt caataaaacc cgctaaaaat
    19741 tatcgattca tcaataaata catcctcaca ccagaccctt tttcaccaat cgacaatcac
    19801 cattacgttt tttatttttg cactgccaaa tagcttgcgc acagtgctgc tttgcggcat
    19861 ttttttaaac aagcaaacac aacaagcaac aaataccagg ttaacggaga aggttatgtg
    19921 ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta agaaagcact
    19981 cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt atgccagcga
    20041 taacgccatt ctcgctcacg aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc
    20101 tctctacaac caacaaaaaa accacgtact ggcggtaaac ggtgaaatct acaaccacca
    20161 ggcattgcgc gctgaatatg gcgatcgtta tcagttccag acagggtctg actgtgaagt
    20221 gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc agggcatgtt
    20281 tgcctttgcc ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg accatctggg
    20341 gatcatccca ctgtatatgg ggtatgacga acacggtcag ctgtatgtgg cctcagaaat
    20401 gaaatcgctg gtgccagttt gccgcacgat taaagagttc ccggcgggga gctatttgtg
    20461 gagtcaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact ttgatgcagt
    20521 gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt ccgttaaaag
    20581 ccatctgatg tctgatgtgc cttacggtgt gctactttct ggtggtctgg attcctcaat
    20641 tatttccgct atcaccaaga aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga
    20701 agcctggtgg ccgcagttac actcctttgc tgtaggtctg ccgggttcac cggatcttaa
    20761 agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc acttcactgt
    20821 acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt atgatgtgac
    20881 cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat
    20941 taaaatggtg ctgtccggtg aaggttctga tgaagtgttt ggcggttatc tttacttcca
    21001 taaagcgccc aacgctaaag aactgcatga agagacggtg cgtaaactgc tggccttgca
    21061 tatgtatgac tgcgcgcgcg ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt
    21121 tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgc attaacccgc aggataaaat
    21181 gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgagt catacctgcc
    21241 cgcaagcgtg gcctggcggc agaaagagca gttctccgat ggcgtcggtt acagttggat
    21301 cgacaccctg aaagaagtgg cggcgcagca ggtttctgat cagcaactgg aaactgcccg
    21361 cttccgcttc ccgtacaaca cgccgacctc aaaagaagcg tatctgtacc gcgagatctt
    21421 tgaagaactg ttcccacttc cgagtgccgc tgagtgcgtg ccgggcggtc cttccgtcgc
    21481 gtgttcttca gctaaagcga tcgaatggga tgaagcgttc aaaaaaatgg acgatccgtc
    21541 tggtcgtgcg gttggcgttc accagtcggc gtataagtaa gaaatgcggt acagccccgg
    21601 agaaatccgg ggctgtttta gttattgcgc tgcttgttga ttctgatatg cagtaaggaa
    21661 atgttccggg tttgccacat cactgtttga caactcatca ctgtaggcca gtatttcata
    21721 gattaatttc ccaccactat tgttgaattc cgaatttcat cctgtacgac aaaagaatgg
    21781 ctgtagggtg gggaaaaaca caaagtaaac aataattgac gaatatagcg ccacgctgtt
    21841 cgcaacctaa ccaaacagtc actttcgagc aattttcctt gaaaaagagg ttgacgctgc
    21901 aaggctctat acgcataatg cgccccgcaa cgccgataag gtatcgcgaa aaaaagatgg
    21961 ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca ggttcgaatc
    22021 ccgtcgtagc caccatcttt tttgcgggag tggcgaaatt ggtagacgca ccagatttag
    22081 gttctggcgc cgcaaggtgt gcgagttcaa gtctcgcctc ccgcaccatt caccaggaag
    22141 cgttgtacgg atggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc
    22201 tggttcgaat ccaggtaccc cagccatctt cttcgagtaa gcggttcacc gcccggttat
    22261 tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct ggttcgaatc
    22321 caggtacccc agccatcgaa gaaataatct ggctacgtag ctcagttggt tagagcacat
    22381 cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccaaat tctgaatgtg
    22441 tcgaatacgt tcg
//
LOCUS       DAJVPP010000065        22281 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVPP010000065 DAJVPP010000000
VERSION     DAJVPP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.065"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1028)
                     /locus_tag="OQM34_002539"
     CDS             complement(<1..1028)
                     /locus_tag="OQM34_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1452933.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTEGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="OQM34_002540"
     CDS             complement(1130..2110)
                     /locus_tag="OQM34_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCS1452934.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="OQM34_002541"
     CDS             complement(2118..2828)
                     /locus_tag="OQM34_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCS1452935.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OQM34_002542"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OQM34_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1452936.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PVVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="OQM34_002543"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="OQM34_002543"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1452937.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="OQM34_002544"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="OQM34_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1452938.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="OQM34_002545"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="OQM34_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1452939.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /locus_tag="OQM34_002546"
     CDS             9405..9515
                     /locus_tag="OQM34_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1452940.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OQM34_002547"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OQM34_002547"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1452941.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OQM34_002548"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OQM34_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1452942.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="OQM34_002549"
     CDS             12397..13029
                     /locus_tag="OQM34_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1452943.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OQM34_002550"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OQM34_002550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1452944.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEEFTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16561..17493
                     /locus_tag="OQM34_002551"
     CDS             16561..17493
                     /locus_tag="OQM34_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1452945.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="OQM34_002552"
     CDS             complement(17534..18964)
                     /locus_tag="OQM34_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1452946.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OQM34_002553"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OQM34_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1452947.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OQM34_002554"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OQM34_002554"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1452948.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="OQM34_002555"
     CDS             complement(20825..21094)
                     /locus_tag="OQM34_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1452949.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="OQM34_002556"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="OQM34_002556"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1452950.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNHAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="OQM34_002557"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="OQM34_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1452951.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5509 a         5587 c         5335 g         5850 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcaccgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agccctctgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacgt ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcacttg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggggcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgaccggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttcctcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gaactcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgcggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgcccg cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    18961 tcatataaat attccctgat aaaacagcag aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgctcaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc tgatgacgac
    21181 gataacaggc gttgtgctgg caggtggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cacgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggcacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg c
//
LOCUS       DAJVPP010000066        21828 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVPP010000066 DAJVPP010000000
VERSION     DAJVPP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.066"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..2380
                     /locus_tag="OQM34_002558"
     CDS             <1..2380
                     /locus_tag="OQM34_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCS1452952.1"
                     /translation="VDIVLNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDA
                     FPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEG
                     INALFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWKNLS
                     SYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVRGIA
                     KSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSVPFS
                     SVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAEYYR
                     AAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQLMGY
                     RYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQLGE
                     YGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVFALN
                     FSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSYSVQ
                     QGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGITLG
                     QSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKRNV
                     DLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNSGII
                     DDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
     gene            2371..3354
                     /locus_tag="OQM34_002559"
     CDS             2371..3354
                     /locus_tag="OQM34_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1452953.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            3452..3994
                     /locus_tag="OQM34_002560"
     CDS             3452..3994
                     /locus_tag="OQM34_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1452954.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4002..4517
                     /locus_tag="OQM34_002561"
                     /pseudo
     CDS             4002..4517
                     /locus_tag="OQM34_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            4483..5221
                     /locus_tag="OQM34_002562"
                     /pseudo
     CDS             4483..5221
                     /locus_tag="OQM34_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            5332..6342
                     /gene="pyrD"
                     /locus_tag="OQM34_002563"
     CDS             5332..6342
                     /gene="pyrD"
                     /locus_tag="OQM34_002563"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1452955.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6516..7058
                     /gene="zapC"
                     /locus_tag="OQM34_002564"
     CDS             6516..7058
                     /gene="zapC"
                     /locus_tag="OQM34_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1452956.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7055..8164)
                     /gene="ycbX"
                     /locus_tag="OQM34_002565"
     CDS             complement(7055..8164)
                     /gene="ycbX"
                     /locus_tag="OQM34_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1452957.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8408..10516
                     /gene="rlmKL"
                     /locus_tag="OQM34_002566"
     CDS             8408..10516
                     /gene="rlmKL"
                     /locus_tag="OQM34_002566"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1452958.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10528..12435
                     /locus_tag="OQM34_002567"
     CDS             10528..12435
                     /locus_tag="OQM34_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1452959.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12565..13818
                     /gene="pqiA"
                     /locus_tag="OQM34_002568"
     CDS             12565..13818
                     /gene="pqiA"
                     /locus_tag="OQM34_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1452960.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            13823..15463
                     /gene="pqiB"
                     /locus_tag="OQM34_002569"
     CDS             13823..15463
                     /gene="pqiB"
                     /locus_tag="OQM34_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1452961.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15460..16023
                     /gene="pqiC"
                     /locus_tag="OQM34_002570"
     CDS             15460..16023
                     /gene="pqiC"
                     /locus_tag="OQM34_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1452962.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16279..16446
                     /gene="rmf"
                     /locus_tag="OQM34_002571"
     CDS             16279..16446
                     /gene="rmf"
                     /locus_tag="OQM34_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1452963.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16516..17034)
                     /gene="fabA"
                     /locus_tag="OQM34_002572"
     CDS             complement(16516..17034)
                     /gene="fabA"
                     /locus_tag="OQM34_002572"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1452964.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17103..18863)
                     /locus_tag="OQM34_002573"
     CDS             complement(17103..18863)
                     /locus_tag="OQM34_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1452965.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19048..19500
                     /gene="matP"
                     /locus_tag="OQM34_002574"
     CDS             19048..19500
                     /gene="matP"
                     /locus_tag="OQM34_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1452966.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19576..20622)
                     /gene="ompA"
                     /locus_tag="OQM34_002575"
     CDS             complement(19576..20622)
                     /gene="ompA"
                     /locus_tag="OQM34_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1452967.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(20979..21488)
                     /gene="sulA"
                     /locus_tag="OQM34_002576"
     CDS             complement(20979..21488)
                     /gene="sulA"
                     /locus_tag="OQM34_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1452968.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21707..>21828
                     /locus_tag="OQM34_002577"
     CDS             21707..>21828
                     /locus_tag="OQM34_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCS1452969.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFA"
BASE COUNT         5567 a         5135 c         5723 g         5403 t
ORIGIN      
        1 tgtcgatatt gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat
       61 aacgccagag aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggtgt
      121 taatactgat gcgtttccgg cttttaaaca actggacaaa caagtgtgtg tgccattggc
      181 ggagattatt ccggatgcca gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc
      241 agtaccgcaa atcgccatca aaagtaatgc ccgtggttat gtcccccctg aacgttggga
      301 tgaagggatc aacgcgctat ttagcggggc taacagtatt catagcagcg cagacagtga
      361 ttctggcgac agctattttc tgaatttaaa cagtggcgtt aatttaggcc catggagatt
      421 gcgcaacaat tcaacatgga gtcgcagtag tggccaaacc gcagaatgga agaatctcag
      481 cagctatttg cagcgggcgg ttattccact aaaaggcgaa ctgaccgtag gtgatgatta
      541 tactgcaggc gattttttcg atagtgtcag ctttcgtggt gtgcagctgg cgtcagatga
      601 caacatgctg ccagacagcc tgaaagggtt tgcgcctgtg gtgcgtggta tcgccaaaag
      661 caatgcccag ataacgatta agcaaaatgg ttacaccatt taccaaactt atgtatcgcc
      721 tggtgctttt gaaattagtg atctctattc cacgtcgtcg agcggtgatt tgttagttga
      781 aatcaaagaa gcggacggca gcgtcaatag ctacagcgta ccgttttcca gcgtgccatt
      841 actccagcgt caggggcgaa tcaaatacgc ggtgacactg gcgaaataca gaaccaatag
      901 taatgaacag caggagagca aatttgccca gaccacgttg cagtggggcg gaccgtgggg
      961 aacgacatgg tatggtggtg gacaatatgc tgaatattac cgtgccgcca tgtttggtct
     1021 gggatttaac cttggcgatt tcggagcaat ttcgttcgat gcgacccagg cgaagagtac
     1081 gctggcagac caaagcgaac ataaaggtca gtcatatcgt tttctgtatg ccaaaacgct
     1141 caaccaattg ggcactaatt ttcaattgat gggctatcgc tattcgacgt cgggtttcta
     1201 caccctttcc gacaccatgt ataaacatat ggatggctac gaatttaatg acggtgatga
     1261 tgaagatacg ccgatgtggt cgcgttatta caatttgttt tacaccaaac gtggcaaact
     1321 gcaggtcaat atctcccagc aattaggcga gtacggttcg ttttatttaa gtggtagcca
     1381 gcaaacttac tggcataccg atcaacagga tcggctatta cagtttggct acaacacgca
     1441 aattaaagat ctctcgctgg gggtttcctg gaactacagt aagtcccgtg gtcaacctga
     1501 tgctgatcag gtgtttgcac taaatttttc cctgccgctc aatctgttgc tccccagaag
     1561 taatgatagc tataccagga aaaaaaatta cgcctggatg acctctaaca ccagtatcga
     1621 taacgaaggg cacattacac aaaacctggg tttaacggag acactactcg atgacggtaa
     1681 cctgagctac agcgtgcaac agggatataa cagcgagggg aaaacggcta atggtagcgc
     1741 cagcatggac tacaaagggg cgtttgcaga tgcccgagtg ggctacaact acagcgataa
     1801 cggcagtcaa caacaactga actacgctct ttcaggcagt ttagttgccc attcacaggg
     1861 cattaccctg gggcaatcgc tgggggaaac taacgtgctg attgcagcac caggcgcaga
     1921 gaatactcgt gtggcgaaca gcaccgggct gaaaactgac tggcgcggat ataccgttgt
     1981 cccctatgcc acttcttatc gggaaaatcg aatcgcactt gatgcggcat cgttaaaacg
     2041 taacgtggat cttgaaaatg cagtagtcaa cgtggttccc accaaagggg cgttggttct
     2101 ggcggagttc aatgcccatg cgggagcaag ggtattaatg aaaacatcaa agcagggtat
     2161 accgctgcgt tttggcgcga tagcgacgct ggacggcata cagactaata gcggcattat
     2221 tgatgatgat ggctcgctct atatgtccgg tttgcccgct cagggagcga taactgtacg
     2281 ctggggcgaa gctcccgatc aaatttgtca tatcagttac cagcttaccg aacaacaaat
     2341 taactctgcg attacgcgaa tggatgccat atgcagataa tctttggaga aaaatgcgtg
     2401 ttattactac gactattttt tgccgccgtc ttaatgctat ggtgcgctca aactgctgct
     2461 tatatcgggc agtgtcatac cactcagggg aatccgtata ttggcgtcaa ttttggcgtt
     2521 aaaactctgg atgaagaaga aaatacggct ggagtagtta aagacaaatt ttatcagtgg
     2581 aacgaatcga atgattatta tgtttcctgt gattgcgata aagacaatgt cagaagtggc
     2641 cgatgggcat tcgccgcgga ttcaccgtta gtctatttag gcgacaactg gtacaaaatt
     2701 aatgactatc ttgccgccaa agttttattg caggttaaag gcagttctcc tactgcggtt
     2761 cctttcgaaa acgtgggcac aggggcagat acacgatggc atatttgcga tcccggcggt
     2821 caacgtttag gtggccaggg ggctagcggt aatagcggta gcttttcact gaaaatattg
     2881 cagccgttcg ttggttcggt cgtcattcct cctatggcgc tggcgcgatt atttgaatgc
     2941 tacaacatac ccgcaggtga ttcctgcacg actactggta caccggtttt agtgtattac
     3001 ctgtctggta cgatcaattc acttggctca tgttccgtca atgccggaga aacaatcgag
     3061 gttgatctgg gcgacgtatt tgcggctaac tttcgtgttg tagggcataa gcctcttggg
     3121 gccagaacgg cagaacttgc aattccggtc aggtgtaaca cgggaaacgc agggttagtt
     3181 aacgtcaatc tgagtctgac ggcaacctca gaccccagct atccccaggc gattaagacg
     3241 tcacgtcctg gcgtgggcgt ggtggtgacc gatagccaga acaacattat ttcccctgct
     3301 ggtgggacac taccgctctc tattcctgat gatgcagaca gtatccgcga atgaatgtct
     3361 atccagtcag cacgacaggc gtaccaccag aaaccgggcg atttgaagcc acggcaacgg
     3421 tgagaataaa ttttgattaa cgcgtgaacg tatgaagaaa aaaacgatat atcagtgcgt
     3481 tattttgttc tttagccttc ttaacatcca tgtcgggatg gctgggcctg aacaagtcag
     3541 tatgcatatt tatgggaatg tggtcgatca gggctgtgat gtcgccacca aaagtgcatt
     3601 acaaaatatt catattggtg attttaatat cagtgatttt caggccgcga ataccgtaag
     3661 cactgctgct gatttgaata ttgatatcac cggttgtgcc gctggtatta ctggcgcgga
     3721 cgtccttttt agcggcgagg ctgacaccct tgcgccgaca ctgctcaaac taactgacac
     3781 aggcggaagc ggtggtatgg caacggggat tgccgtgcaa attcttgatg cgcaaagtca
     3841 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     3901 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     3961 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4021 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4081 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4141 tcaatgtgca gttaggcgat gtctcgctga caggtttttt tcatgggaat tatggcccgg
     4201 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4261 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4321 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4381 actaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4441 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4501 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4561 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4621 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4681 attaacagca aaatcaatcg cccaacgcgt tgggccgggg ggggaagcgt cgacagtgcc
     4741 agcactatta ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt
     4801 gcgcttgctg agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt
     4861 aagcattgcc agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg
     4921 ctcggtattt aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta
     4981 tcaacaatta cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta
     5041 ctacattaac ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagtcgt
     5101 gccgcctaaa agccagcgtc agacaaactg gtgtcaggta atagcacggt gtcatgtcgc
     5161 ctggcggacg ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg
     5221 aaacaggttc tgaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga
     5281 actcgcacat aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac
     5341 cccttcgttc gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt
     5401 cagcaattac gccgcattac cggaacaccg tttgaagcac tggtgcggca gaaagtgcct
     5461 gcgaaacctg ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt
     5521 cttgataaag acggggagtg cattgatgcg ttaggcgcga tgggatttgg atcgatcgag
     5581 atcggtaccg tcacgccacg tccacagcct ggtaatgaca agccgcgtct ctttcgtctg
     5641 gtagatgccg aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc
     5701 gtagagaacg taaaaaaggc ccattatgac ggcgtcttgg gtattaatat cggcaaaaat
     5761 aaagatacgc cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat
     5821 gcctatgcgg gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg
     5881 ctgcaatatg gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat
     5941 ttgcaagcga tgcaccataa atatgtgccg atcgcagtga agatcgcgcc ggatctttct
     6001 gaagacgaat tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt
     6061 gcaaccaata ccactctcga tcgttctctg gttcagggaa tgaaaaattg cgatcaaacc
     6121 ggtggcttaa gtggtcgtcc gcttcagtta aaaagcacag aaattattcg ccgcttgtca
     6181 ctagaattaa acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct
     6241 gcgcgtgaaa agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt
     6301 aaaggtccgc cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct
     6361 ctttataaga taatcagggc tttattttca gccctggttg ttttatattc atcgttgttg
     6421 cttatttaga cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg
     6481 tgttgctgcg gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact
     6541 ggcgttggta ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc
     6601 tatttcgctc acgttttgcg cgcaaaatgt tgactccaga tgccttttct cccgcaggct
     6661 tttgtgttga tgatgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt
     6721 tatctaaaga ccaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgttatctca
     6781 aaccgcaaat gccgaaaagc tggcattttg tctcccatgg tgaaatgtgg gtgcccatgc
     6841 cgggcgatgc ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg
     6901 ttgaatctgg cgaaaatgcc gcgctatgcc tgctggcaca accctgcgtt gttattgcgg
     6961 gtcgcgcgat gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg
     7021 tgaatgttga cagcttcagc ctcgaacagg cagtctaacg cgtcagctta agggcagtct
     7081 tcggtacaca gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg
     7141 gcgtgacttc gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag
     7201 aataagggat acgaattccc tgattttcta attgctccag caacacttgt tggttatttc
     7261 cacgaaatgc ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt
     7321 tggcggtatc atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt
     7381 ccacctcatc gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat
     7441 cgacatcgcc gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg
     7501 ccggatgttt ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt
     7561 taaccacatc aaacaccaca tcaccaatgc gaatcacttt ccagctatct tcttcccagg
     7621 ctgacgcgcc ggaaaccacc agattggtgc ggaattgctc catttttaca ctggccggac
     7681 aacgttgttg gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag
     7741 caaatgacag aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc
     7801 agcgtaattg cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg
     7861 caattcgcgc ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat
     7921 cagcaaaacg aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca
     7981 caggtgaagg agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt
     8041 caggttccgt gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat
     8101 gtgtaagacc aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg
     8161 ccacggtgcg gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta
     8221 gctataatgc gcaacaattt tcttagcgcc tgtcccgtta ggctatttta tttgtcagtt
     8281 tggccctggg ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct
     8341 ctccatgtcc agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag
     8401 tgacgatatg aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac
     8461 tgaactggaa aatctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa
     8521 gggcgacaca cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat
     8581 gttgccgctg ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc
     8641 gatcaactgg acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt
     8701 gaatgacacc atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga
     8761 tgctttcacg cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg
     8821 cgttaacgtc tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg
     8881 tttacatctg cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc
     8941 agccgctatt gtgatgcgat ccggctggca gtcaggaaca ccgctgctcg atccgatgtg
     9001 tggttccggt acgttgctga ttgaagcggc gatgctggcg accgatcgcg caccaggctt
     9061 gcaccgtggg cgttggggct ttagcggctg ggcgcagcat gatgaagcta tctggcagga
     9121 agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggcctg gctgagtata gctctcattt
     9181 ttacggttcg gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc
     9241 ggggattggt gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct
     9301 gccgaaaggg ccgtacggta cagtgttgag caacccgcca tacggtgaac gtctggacag
     9361 cgaaccggcg ctgattgcgc tgcatagtct gctgggccga atcatgaaaa accagttcgg
     9421 tggctggaat ctctctttgt ttagtgcctc gccggatctg ctaagctgcc tgcagctgcg
     9481 tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca
     9541 cgttgccgaa agcacaccag acagtaaacc ggcaatggta gcggaagatt acgccaaccg
     9601 cctgcgtaag aacctcaaaa aattcgagaa gtgggcacgc caggaaggga ttgaatgtta
     9661 ccgcctgtat gacgccgatc tgccggaata taacgttgcc gttgaccgtt atgccgactg
     9721 ggtggtggtg caggagtatg cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg
     9781 tctgttcgat attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt
     9841 gctgaaaacc cgtgaacgcc ataagggcaa aaaccagtac cagaaactgg gcgagaaggg
     9901 cgagtttctt gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct
     9961 cgatactggt ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa
    10021 aggcaaagat ttcctcaacc tgttttctta taccggcagc gccaccgtgc atgcgggatt
    10081 aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg aatgggcaga
    10141 acgcaacctg cgtctgaatg gcctgactag gcgtgcgcat cgcctgattc aggccgattg
    10201 cctggcgtgg ctgcgtgagg caaatgaaca gttcgatctg atctttatcg atccgccaac
    10261 cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct
    10321 gatgaaagat ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa
    10381 acgtggcttc cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat
    10441 tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct
    10501 gattaccgca gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg
    10561 tcgttcagcg acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt
    10621 gtttgtctgg tgggccgcaa cggcgcagga aaatcgacgt taatgaaaat cctcaaccgt
    10681 gaacaagggc tggatgacgg tcgcattatt tatgagcaag atttgattgt agctcgtctg
    10741 caacaggatc caccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt
    10801 gaagaacaag cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac
    10861 ccgagcgaga aaaatctcaa cgaactggcg aaggttcagg aacagctgga tcaccacaac
    10921 ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaac
    10981 gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg
    11041 gtgagcaatc cgcgcgtatt gctgctggac gaaccgacta accatctgga tatcgaaacc
    11101 atcgactggc tggaggggtt ccttaaaacc ttcaatggga cgattatttt catctcccac
    11161 gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg
    11221 gtgacctatc cagggaatta cgaccagtac ctgcaggaaa aagaagaagc cctgcgcgtg
    11281 gaagaactac aaaatgccga gttcgatctc aaactggcgc aggaagaagt gtggattcgc
    11341 caggggatca aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcgatg
    11401 cgtcgcgaac gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag
    11461 gccagccgct ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttaacggt
    11521 aagcaactgg tgaaagattt ttctgcccag gttctacgtg gcgacaaaat tgccctgatt
    11581 ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactga tgctcggtca gcttcaagcg
    11641 gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc
    11701 gcggaactgg atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg
    11761 atggttaatg gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg
    11821 aaacgggcga tgacgccagt acgtgcgttt tctggcggtg agcggaaccg cttgctgctg
    11881 gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt
    11941 gatgtcgaaa cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg
    12001 ctggttagcc acgatcgtca gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa
    12061 ggcggcggta aaattggtcg ttatgttggc ggttatcatg atgcccgtgg tcagcaagaa
    12121 caatatgtgg cactcaaaca gcctgcggtg aaaaaaagcg aagaagccgc cgcgccaaaa
    12181 gcagaaactg taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag
    12241 cagctaccgc aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaagtg
    12301 gcggatgctt ccttcttcag tcagccgcat gagcagacgc aaaaagtgct tgctgatatg
    12361 gctgctgcag agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta
    12421 aaaaatggtg gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa
    12481 ttgcctgatg aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa
    12541 atagcgcatc attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg
    12601 tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca
    12661 tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc
    12721 gcctatgcgt tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat
    12781 atgaacgttg caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt
    12841 tctgaggact acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg
    12901 ttttgtctga taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa
    12961 gagcaactgg cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc
    13021 gcgggtgtgc tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc
    13081 agctttctcc cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat
    13141 cgtcgctggt tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca
    13201 ggcgtcacgg ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct
    13261 gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc
    13321 ctgcagtgga cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt
    13381 ttgcccatca tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg
    13441 gtcattctgt tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt
    13501 attatggtgc caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg
    13561 catggcaagc gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc
    13621 cgctggtcga tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga
    13681 ggtttaatga gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg
    13741 acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca
    13801 gagcatgagg agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt
    13861 gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt
    13921 tcttttttat cattacagcc atcagggacc ggaagtgacc ctgatcaccg cgaatgcgga
    13981 aggaattgaa ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga
    14041 aagcgccaca ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg
    14101 tatggaaaaa ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg
    14161 cgaagggatt agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg
    14221 cgcgaaaggc agcaaaatgg ataaatacga tttgctggac tcaccaccgt tggccccgcc
    14281 tgatgcgaaa ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg
    14341 agatccggtg ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac
    14401 gcaaaaacgc aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac
    14461 cagcaatgtt cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat
    14521 gcgtgtggag atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc
    14581 ggaaggtctg gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga
    14641 tcagaagagc attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa
    14701 agattcggta cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg
    14761 taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta
    14821 ccgtattccg gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa
    14881 tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa
    14941 aaccggaaac ctggtcactg gtgcactgta tgttgatctc gatttctatc caaatacgcc
    15001 tgcaataacc ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg
    15061 cttggcgcaa atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt
    15121 gaatccgatg attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa
    15181 cctgcaaacg acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt
    15241 gccgacggat atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc
    15301 tggctccgca gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg
    15361 agaactgcaa ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc
    15421 gaaggacaaa aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg
    15481 attgcagcac tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag
    15541 ttacctgtgg tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg
    15601 gtagagcagg tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt
    15661 gatgtgaagt atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacagttg
    15721 cgcaacaccc tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag
    15781 cctctgggaa gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat
    15841 gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa
    15901 cgtccgttcc gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagtg
    15961 ctggccggtg tctggagtca ggaagccgct tctattgcac aagagataaa gcgtctacct
    16021 taattataaa gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg
    16081 tttctaacgt gcggaaaaat ttgttcctct tcacattttt tgtacaaccg acatgcccgt
    16141 gtagctcaca aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg
    16201 gtattgctta actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca
    16261 gtatgaggga aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa
    16321 cgtggttatc aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg
    16381 agtcaaaggt cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg
    16441 gcctgattct gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac
    16501 tctgtctgat gataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct
    16561 ggcggtatag atcagacgac aatcaaccag cacttcgcca tctgccagac ccataatcag
    16621 acgacgatta acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag
    16681 tacctgacca gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc
    16741 cagccagccg aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg
    16801 cataaccgga tcgccaatga agtggcatcc gaagaaccac agatccggat tgatatccag
    16861 ttctgcttca acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg
    16921 gtctatcatc agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc
    16981 gtgaccagag gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc
    17041 tgtaagcctt attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc
    17101 gatcagttcg gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct
    17161 tcctgttgcg atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct
    17221 tcgccatccc acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc
    17281 cagatggtga atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg
    17341 cgaacgttag cagtggggat aatgacacct tgtttccccg ttaactcacg ttgctggcaa
    17401 atagcaaaga agccttcgat tttctcattt aaaccaccga ccggctggac gcggccgaac
    17461 tgatcgactg aacctgtgat agcgatactc tgattcactg gcacatcagc gagggcgctt
    17521 atcagggcgc agagttcagc catcgaggca ctatcgccat ccacttcact gtatgactgc
    17581 tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg
    17641 aacgcctgca tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt
    17701 tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg
    17761 ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg
    17821 cgttcgcctt cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca
    17881 gcgaggaaac cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag
    17941 aaggtgtcgc catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg
    18001 ctaagcggaa gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc
    18061 caagcatccg ctcccggggc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga
    18121 caccactggc ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa
    18181 atagcctgct ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg
    18241 cctaccagaa tgactttcag cttcaatggc atcgaaggca cagagacggg gagagggcgc
    18301 gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc
    18361 cacagcagag gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc
    18421 tgatgcacca gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag
    18481 agttgctccg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca
    18541 tctgcactca ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgatcgccc
    18601 accagttggc ctgcatcgct ttgtagcgta cgcgcggcat cggcaataag attcagatac
    18661 tcagactctt ccggggcctt agccagcata aaagaggagg atgctcgcgt atgcagcaac
    18721 tgctccagcg caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca
    18781 atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc
    18841 catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt
    18901 ataccgtaac cggcactaca gcacacgtag aaagcaccga caatactcct ggctgggcgt
    18961 taaagctcac aggatggaga ttcttttctt cactggccta aaaagctgat attctgtaaa
    19021 gagttacacg gtaacattga gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc
    19081 ggttggaaat ggaagtatct ggtgaagaag catcgcgaag gggagttaat tacccgttac
    19141 atagaagcca gtgccgccca ggaagccgtc gatgtgttgc tctcgctcga aaatgaaccc
    19201 gtgctggtaa atggctggat tgacaagcat atgaatccgg aactggtcaa tcgcatgaag
    19261 cagaccattc gggccagacg gaagcgccat tttaatgcgg aacaccagca tacgcgcaaa
    19321 aaatcgatcg atctggaatt tattgtctgg caacgtctgg ctggtcttgc acagcgtcgc
    19381 ggtaaaacgc tttctgaaac gattgttcag ctgattgaag atgcggaaaa caaagagaaa
    19441 tacgcgaata aaatgtcttc tcttaagcag gatctgcagg cattgctggg taaggaataa
    19501 ctgacgaaag tcagttcaat ttactaaagg caaaaaaaac cccgcagcag cggggttttt
    19561 ctaccagacg ataacttaag cctgcggctg agttacaacg tctttgatac ctttaacttc
    19621 gatctctacg cgacgatccg gagccaagca gtcgatcagt gcagcacgct gtttcacgtt
    19681 gtcacaggtg ttgccagtaa ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc
    19741 tgccgggata cctttggaga tcaggtaatc aacaacagac tgagcacggc gctcggacag
    19801 accctggttg taagcgtcag aaccgatgcg gtcagtgtaa cccagaacaa ctacggaacc
    19861 gtctttcgga tctaggttgc tcagctggct gtacagctga tccagagcag cctgaccttc
    19921 cggtttcagg gttgctttgt tgaagttgaa cagaacgtca gacttcagag tgaagtgctt
    19981 ggtctgtact tccggtgccg gagctggagc aactaccgga gctgcttcgc cctgaccgaa
    20041 acggtaggaa acacccaggc tcagcagacc gttgtccgga cgagtaccga tggtgtttgc
    20101 gtcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc
    20161 gtactcaaca ccacctgcga aaaccggaga aacgccagtg tcgtggtctt taaaggatgc
    20221 gccaccaggt acgttagcct tggtgtctgc acgccatacc ataccaccca gacgagtgta
    20281 gatgtccaga tcgtcagtga ttgggtaacc cagtttagcg gtcagctgaa cgccctgagc
    20341 tttgtatgcg ccgttgatgt tgtcgccttt gtacggcata cgacctaacc agtcgtaacc
    20401 catttcaaaa ccaacatacg ggttaacctg gtaaccacca aaagcacctg cacccagttg
    20461 gttttcgtgg gtcggaccat tgttaggaat aaaaccagtg tcatggtact gggaccagcc
    20521 cagtttagca ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa
    20581 accagccagt gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc
    20641 atccaaaata cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg
    20701 atctgctcaa tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt
    20761 aaagtctaca acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa
    20821 agtcttgtat aaggtatgtt taatcttttt gtcagcgaca atttacagaa aaaaaacgcg
    20881 gaaaccgctt cagacaagcc ttcgcaagga aaattagtca cgactgaaag cattggctgg
    20941 gcgacaaaaa aaagttctag gattaatcct aaatttactt aatgatacaa attagagtga
    21001 atttttagcc cggaaagttg tctcgttgtg tgagaggatg cgcttaccgg acgcataata
    21061 aaccccatag cgttaccttc atttgccgca tcaacaagtt cagcatgctc ttcttcagtc
    21121 aaatcatctg tcaaccaacc gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc
    21181 attgactcca ctgtgtggca aggggagagc tggctaatct gcattacttt cgttaagggt
    21241 agcccagatg cctgaaccca ttcccgactc agtttttgtt gcggtgttaa ccagagttgc
    21301 cagcgcgatt gctgaccgag ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg
    21361 ggctgatctt cgcgatagac aacttcactg ataagcccgg ctgtagtgtt ttccgtagag
    21421 acacgcgcaa ttttacttgc tgcggatgag aacgacgaag aacgatgtgc atagcctgaa
    21481 gtgtacataa tcaatccagc ccctgtgagt tactgtatgg atgtgcagta catccagtga
    21541 caacaaagat caaccctatt ttcggaaaga gcctcgcaaa ttttgtcgtt ggtgacggga
    21601 aaacataaat taatcttgcc ccttaagaat aagttgccta ttttcgtagt taacggatcc
    21661 gttaatgtga atcattcttt tatgttatga ttttaaaagg aattttatga aaagcctctc
    21721 ctataagcgg atctataaat cacaagaata cctggcaacg ttgggcacaa ttgaataccg
    21781 atcattgttt ggcagttaca gcctgaccgt tgacgacacg gtgtttgc
//
LOCUS       DAJVPP010000067        21753 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVPP010000067 DAJVPP010000000
VERSION     DAJVPP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.067"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            502..1401
                     /locus_tag="OQM34_002578"
     CDS             502..1401
                     /locus_tag="OQM34_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1452970.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDVTSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            1450..2463
                     /gene="yhjD"
                     /locus_tag="OQM34_002579"
     CDS             1450..2463
                     /gene="yhjD"
                     /locus_tag="OQM34_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1452971.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            2874..4196
                     /locus_tag="OQM34_002580"
     CDS             2874..4196
                     /locus_tag="OQM34_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1452972.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4378..6438)
                     /locus_tag="OQM34_002581"
     CDS             complement(4378..6438)
                     /locus_tag="OQM34_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1452973.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(6508..7275)
                     /gene="pdeH"
                     /locus_tag="OQM34_002582"
     CDS             complement(6508..7275)
                     /gene="pdeH"
                     /locus_tag="OQM34_002582"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1452974.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            7507..8436
                     /gene="kdgK"
                     /locus_tag="OQM34_002583"
     CDS             7507..8436
                     /gene="kdgK"
                     /locus_tag="OQM34_002583"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1452975.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(8532..10028)
                     /locus_tag="OQM34_002584"
     CDS             complement(8532..10028)
                     /locus_tag="OQM34_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1452976.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10249..11535)
                     /gene="dctA"
                     /locus_tag="OQM34_002585"
     CDS             complement(10249..11535)
                     /gene="dctA"
                     /locus_tag="OQM34_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1452977.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(11720..13708)
                     /gene="hmsP"
                     /locus_tag="OQM34_002586"
     CDS             complement(11720..13708)
                     /gene="hmsP"
                     /locus_tag="OQM34_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1452978.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(13790..15538)
                     /gene="bcsC"
                     /locus_tag="OQM34_002587"
                     /pseudo
     CDS             complement(13790..15538)
                     /gene="bcsC"
                     /locus_tag="OQM34_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            complement(15866..16042)
                     /locus_tag="OQM34_002588"
                     /pseudo
     CDS             complement(15866..16042)
                     /locus_tag="OQM34_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            complement(16101..17207)
                     /gene="bcsZ"
                     /locus_tag="OQM34_002589"
     CDS             complement(16101..17207)
                     /gene="bcsZ"
                     /locus_tag="OQM34_002589"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1452979.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(17214..19556)
                     /gene="bcsB"
                     /locus_tag="OQM34_002590"
     CDS             complement(17214..19556)
                     /gene="bcsB"
                     /locus_tag="OQM34_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS1452980.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRRLNPDNE"
     gene            complement(19567..21753)
                     /gene="bcsA"
                     /locus_tag="OQM34_002591"
                     /pseudo
     CDS             complement(19567..21753)
                     /gene="bcsA"
                     /locus_tag="OQM34_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
BASE COUNT         5083 a         6040 c         5550 g         5080 t
ORIGIN      
        1 atacgttaaa aagacttaag tagtggaagg gtattacccg cgagaaaaaa taagaattcg
       61 ccatttggcg gtggccattc tacagagatg acgcgtagaa aatagttacc gatataaata
      121 gttacagcta aacgcctgaa attacatgtc gagggcacta tttaaaacaa ttttgaggat
      181 ttccttatat tggtggttag tacgcatgca attaaaaatg aaattccgcg actacaagcc
      241 aaaataacaa atggcaatga gacaaaaata agcacaaata gccaatacgt cctctattca
      301 ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg gattcacctt tctcataaag
      361 ctattccgcc cttttcctgc tgagaaatcg ccacattcgt catgacaaca ttgtgaaacc
      421 cggcattaga tgttagaaaa aacaataaac aatgcgatat gcgcgttact gtttcgctgt
      481 gaacaacaat cggtcaaaga aatggataaa attcacgcaa tgcagttgtt catcaaagtc
      541 gcggagctgg aaagtttttc ccgcgcggcg gatttctttg ctttgccaaa gggaagtgtt
      601 tcgcgccaga tacaggcact ggaacatcaa cttggcaccc agcttctcca gcgcactacg
      661 cgacgggtca aactcacgcc agaaggcatg acctattatc aacgggcaaa agatgtgttg
      721 agtaatctca gcgaactgga cggtctgttt caacaggatg tcaccagtat cagcggtaaa
      781 ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt tactgccgcg tctgtcggaa
      841 tttctctatc agcatccggg aattgagctg gaactgagta gccataaccg tccggtagat
      901 attcttcatg atggttttga ttgcgtgata cgcactggcg cgttaccgga agatggcgtt
      961 atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg ccagtccgca ctatctgacg
     1021 cgctttggtt atccgcaaag ccccgatgat ctgacttcac acgcaatagt gcgttataca
     1081 ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca gcgttaatgg cgtccagtgg
     1141 tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa actatctcgc cgccggtctt
     1201 gccggtctgg ggattattca gatcccgcgc attgccgtgc gcgaagccct gcgtgccggg
     1261 cggcttattg aagtgttgcc aggctaccgt gccgagccgc tctccctttc gctggtttac
     1321 ccgcagcgtc gggagctttc ccggcgtgta aacctgttta tgcagtggct ggctggcgta
     1381 atgaaagagc acctggactg accgactata ctttttaaga atgaccacga cagaaggaca
     1441 aaagagcgga tgacgcagga aaacgagatc aaacgtccca cccaggatct ggagcacgag
     1501 ccgattaagc agttggataa tagcgagaaa ggcggcaaag ttagccaggc gctggaaacc
     1561 gtcaccacca ctgccgaaaa agtccaacgc caaccggtta ttgcgcacct tattcgcgcg
     1621 acagaacgct ttaacgatcg gctgggtaac cagtttggtg cggctatcac ctatttctca
     1681 tttttgtcga tgatcccgat tttgatggtg tcgtttgccg cagggggctt tgtgctggcc
     1741 tcccatccga tgttgctaca ggatatcttc gacaaaattc tgcaaaacat cagcgatccg
     1801 acactagccg ccacgttgaa aaacaccatc aacaccgccg ttcagcagcg tacgactgta
     1861 gggcttgtcg gcctggcggt ggcactttat tccggcatca actggatggg taacctgcgt
     1921 gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct cgccgcaaga tcaggagaag
     1981 ttctgggtaa aatatctgcg tgattttatt tcactgattg gtttgttgat tgcgctgatt
     2041 gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc aaatgattat tagcgccctg
     2101 cacctgaaca gcattgagtg gctgaaaccg acgtggcgat tgattggcct ggcgatttcc
     2161 atcttcgcca actatctgct tttcttctgg atcttctggc ggttgccgcg ccaccgtccg
     2221 cgcaaaaaag ccctgattcg cggaacattt ctggctgcta ttggttttga agtgattaaa
     2281 atcgtgatga cctacaccct gccatcgctg atgaaatccc cctctggcgc agcatttggt
     2341 tccgtgctgg ggctgatggc atttttctac ttcttcgccc gtttgacgct gttttgcgcg
     2401 gcgtggattg ccaccgccga atataaagac gacccgcgaa tgccagggaa aacgcagcct
     2461 taaaataggc cggatgcggc gtaaacgcct tatccggcat acgattttat tcggcctggc
     2521 tccccgtagg ccggataaga tgcgccagca tcgcatccgg ctataatgcg cacataacct
     2581 cttgaaactc ttccccagag cctcttcagc catctatttg ggagcaaaca atttcactcc
     2641 aactcataac cccagcatat aaatccagtt ggtaactttt atttaacctg aaaccagttt
     2701 tatccactat ttataaaatt atgtgaagca tttcatagaa gaaaaatcac tggcctaaac
     2761 attatcccct ttttgcctgg tttttgacca tttccgcgat ttgttacaca ttgaaatatc
     2821 acttttgctg tgcgtaatat ggctattcgt tagccaaaaa ataagaaaag attatgcaag
     2881 caacagccac aacactcgac cacgaacaag aatacacgcc gatcaactcg cgtaataaag
     2941 tccttgtcgc ctctctcatt ggcacggcca ttgagttctt cgacttttac atttacgcca
     3001 ctgcggccgt tattgtgttt ccgcatatct tcttcccgca gggcgatcca acggcagcaa
     3061 cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc gcgccccatt ggctctgccg
     3121 tttttggtca ttttggcgat cgcgttgggc gtaaagcgac gctggtcgcc tcgttgctaa
     3181 cgatggggat ttcgaccgtg gtgattggtc tgctgccggg ctatgccacg attggtattt
     3241 tcgccccact gctgctggcg ctggctcgat ttggtcaggg tctgggctta ggcggtgaat
     3301 ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc gcgcaaacgt gcactgtatg
     3361 gctccttccc acagctaggc gcaccgattg gcttcttctt tgccaacggc actttcttgc
     3421 tgctttcctg gctactgacc gatgagcagt ttatgagctg gggctggcgt gtgccattta
     3481 tcttctcggc ggtgctggtc attatcggcc tgtatgttcg cgtgtcgctg catgagtcgc
     3541 cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa gatcccgctg ggtacgctgc
     3601 tgaccaaaca tgttcgcgta accgtactgg gtacgttcat tatgctggca acctatacgc
     3661 tgttttacat catgacggtc tactctatga ccttcagtac cgccgccgcg ccagttgggc
     3721 ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat ggcagttatt ggttttggcg
     3781 tgatggtgcc agtcgccgga ttactggctg atgcctttgg tcgccgtaaa agcatggtga
     3841 tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt taacccgctg ctcggttctg
     3901 gcaacccgat tctggttttt gccttcctgc tgctggggtt aagtctgatg gggctgacct
     3961 tcgggccgat gggtgcgctg ttaccagagc tgtttccgac agaagtgcgt tacaccggag
     4021 catcgttctc ttacaacgta tcgtcgattc tcggggcttc cgttgcgccg tatatcgcag
     4081 cctggttgca ggctaactac gggttaggtg cggtggggtt atatctggcg gcgatggctg
     4141 gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg acatcagtcg ttgtaataag
     4201 tgtgaaaagc ctgatgcgct acgcttatca ggactgaatt tccatacaat atattgaatt
     4261 cacaaggatt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa caaagcacac
     4321 tttgccaaaa atctaaatct gtttaattgc cggatgtcag acatccggca attatcatca
     4381 cttcttcatc tgcgacaata tcgtccgaca ctgattagcc tccccttcgg aaggtgagat
     4441 cagtgccagt aacgccgccg ccggtgttac cagcgttgcc agtgccgcag caacagcacc
     4501 acgggcaatc agcggtccgg ctttcacccc agcctgcgga tttttaaacg tcccacgcac
     4561 atacagcggc gaacgcagtg tgataatccg aatcccttta ctctccggat caatagtcaa
     4621 atccagctgt tccgaagcaa aacttgccgt gccggtaaca ttaatcaacg cgttctcagt
     4681 atcgaaagca aaaatctgcg ggcgcgccac gccgttggca atattcagat tcgccgccgc
     4741 gcagttcacc cgcacctcat cgtcaccaaa tatcgcaccg acaatgtagt tgccgacatt
     4801 cagcccaaca atctccatca ggttgcggct caccagcccg tcattcatca acagtttcag
     4861 gttgccgtta ctgttgccta aaagcgccgc caccgagtta ccgctaccgc gtagttccgc
     4921 gtcaccgttc atttccccca gcgtcttctg catcagttcc acatcgggca tcagttcttt
     4981 cagtttcagt cgacgagcct gaatatctgc ccgcccctgc atcggctttt tatcgccttc
     5041 cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca aatttcagcg gttgcaggcg
     5101 caggtcagca tttttgagga tgatatgagt agaaagatcg ctaatcggca ggctactgcc
     5161 atgctcaatg cgccgccctt tgaagcgaac atcggcatcc ataacgtccc atttatcggt
     5221 ttcgaagcgg tcataaggca gcactttgcc cgcaggctga acgctttttt cgcccttctt
     5281 ctgttcagac cgtttcgact tttctgcccc tttcccggaa tcaacgccaa tcaacggtcc
     5341 caggtccgcc agccgcaatt gccgcgactc gacatcacct tccagttttg gtcgtggctt
     5401 tccggtggtg tagaccagag aaccgtggat atcgctatcg ccaattcgcc cattaaaacc
     5461 gcgataatca aagaccgacg atttttcagt gtcgattttc gctaccagcc gaccatccgt
     5521 ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt tcatagagat cacccagtga
     5581 atcgccagaa aatttaagcc gtaaatcgac accgcccatc ttcattgggt cattcacgac
     5641 gccatcaaaa gcaacacggg tattacctga acggaaatca gcctgtaccg gaaacggcgt
     5701 cccttcgcca cgcagcgcca gcataccgcc tatttttccc gtacccgtga gcggttcacc
     5761 gttataacgt ccctgcgcct tcaagccaaa aatgtaatcg cccacctttt ctttatccgc
     5821 tttacctttc gatccagtaa cttcgctgaa tggcagcggc ttgcctaacg gatcaacaaa
     5881 aatttccaga tccgctttgc ttactttgtc atcaatggcg atccgccctt gatcgaaaag
     5941 aatattatcc agccgaaacg accatgccga cggctttgca ttcgcgtctt tgttgtcatc
     6001 gttggcaaga ttaaacgtcc agttattgtt cttttcagag aggcgaatca ggcgcgcgtc
     6061 gggcttttcg agcttgatcc acggcagcca gaccgttttg gtcagcagcg ccagcggtgc
     6121 cagcgttgcc tctactcgcg gcaaatgcac catcgtgact tcgggaatat ccggtggatt
     6181 accaagaatg atgtcttctg catgtacatg aggccacggc acccagctgc gccagccagt
     6241 ttcttgtttt tgccgctccc acaccacgcc cagattgcca cggatagcga acggacgatt
     6301 caactccgca gagactttct ggttgatggt cggtttgagt cgattccagt caaaagttgc
     6361 aatcaaaatg atcgccacga caatcaacaa caagaaagcc cctgaaatcg cagcggttat
     6421 tttgcctgcc ttgctcatcg ttcgcttcct catattcttc ctgtgccagt cctaaagata
     6481 gtccagcccg gcggaaaatg aggcagctta tagcgccaga accgccgtat tcagcgtttc
     6541 catcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg aatgcgggcg agttctgaac
     6601 atcacgccac tcttccggtg tttctacgcc ctcgacaatc accccgcgac aatagcgatt
     6661 cattagatgt aaaagctgag aaaagagtgt gcggccttcc ggcgactgac gcagcatcac
     6721 aaacagttct cgcgcgattt tgatgtagtc ataacgcact tcacttagtg cagagaaatt
     6781 tgccatcccg gtaccaaaat catccagcca cagcgggcca aattcacaca tcgaggcaaa
     6841 ggttgaatct ttcggcagac ggatatgctc caccagttcg aaacgcagcc agggaagacg
     6901 ctcaatctgg cgcaggattt ttggttgctg acgcagggcg atgagcgtag ggccatcaat
     6961 attgaccgat gccagcaggc cgtgctctac aaagaagtcg gctttttgcg ccagcaaatc
     7021 aatctgctct ttcacaacct ccatccgatg gctgacggtg atttcagtaa aatagcgatc
     7081 cggcggcagg cgttgcgaag ggttcaaggg atgcgtgacc accgttaata gctccacggc
     7141 cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg taagcacgct cacactgcaa
     7201 ccaaaaacgc cgttcctgca agctctcgat gcttgcttca gggttgctta ttcgctggat
     7261 aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg atgctgcccg gactcgacaa
     7321 agattatcgg cgcgtaaggg cagaacttta ccgcctggcc ggggtaaatt tcctcactcc
     7381 acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa taatggaaca ctgttttaat
     7441 atggttgacc agcaaaccac cacagcgcaa actaacgcta attttttaca gatcaggttc
     7501 acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca tgattgagct ttccgagaaa
     7561 ggcgcggacg ttaagcgcgg tttcggcggc gataccctga acacttccgt ctatatcgcc
     7621 cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa cggcgctggg aacggacagt
     7681 tttagccagc agatgctgga cgcctggcac ggcgagaacg ttgatacttc cctgacccaa
     7741 cggatggaaa accgtcttcc gggcctttac tacattgaaa ccgacagcac cggcgaacgt
     7801 acgttctact actggcgtaa cgaagccgcc gccaaattct ggctggcgag cgagcagtct
     7861 gcggcgattt gcgaagagct ggcgaatttc gattatctct acctgagcgg gattagcctg
     7921 gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc tgctgcgcga atgccgcgcc
     7981 aaaggcggaa aagtgatttt cgacaataac tatcgtccgc gcctgtgggc cagcaaagaa
     8041 gagacacagc aggtgtacca gcaaatgctg gaatgcacgg atatcgcctt cctgacgctg
     8101 gacgacgaag acgcgctgtg gggtcaacag ccggtggaag acgtcattgc gcgcacccat
     8161 aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg attcttgcct ggtgtccatt
     8221 gctggtgaag cgttagttga tgttccggcg gtgaaactgc cgaaagaaaa agtgatcgat
     8281 accaccgcag ctggcgactc tttcagcgcc ggttatctgg cggtacgttt gacgggcggc
     8341 agtgcggaaa acgcggcgaa acgcgggcac ctgaccgcaa gtaccgttat tcagtatcgc
     8401 ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc agcatatgca aaaggctgga
     8461 taaagtgtta cccctctatc cagctcatcc ataaatacct gatggttgtg ctaactcatc
     8521 accatcaggc attactgtgc aggtggaata tccgtcactt caggatgtac gtcatccgtg
     8581 gcgacagagg tggtcgcagc ggcggtagat ggggccatga tttgatccca ggccgcctgc
     8641 aacgctttca tgttaaattc cggctcgcct ttcggctgca gcagtattaa cgccatatca
     8701 ttcgacaact gctgacgcag atcctgattt aacatctcca cagtcaggct gttaaggaaa
     8761 tcctgccgca gtttctgata ctgctccggc gcgatatcga cgacctgatt ttgcaacgaa
     8821 cgcatccgct ggcccatcag aatatcggta tcagcccgtg cataggcggc aaacagtttc
     8881 tgcagctcca gtttcttttg cgccactaac gcattgaact cttcttccgg cagaccttta
     8941 tcgcgaacct tcgccagttc acgcgccacc agattaaggt tgctgttcag cttgtcgttt
     9001 ggtgattcga tgttgatggc acactgcgca cgcagataca gcacacggca gtcaaatcca
     9061 agaccgatgt ctttgctgtc actggcgctt aacgcttgct gaacatgcca gaacagcgcc
     9121 tcgcgggcca ggtccgcacg ccagtagcgc agcagtgcgg ctgattcacg aatcggctgc
     9181 cacggcgtat cccacatgat agataaccgg tcctgacgca ccgcgtcagt cataatactc
     9241 accgcttccg cacgcagcgg agaaagcgtc ggcaccggag ctggcgtttc acgtttgcct
     9301 ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg agcgcgcatc cacgtttccc
     9361 accaccagta gcgtcattgc atccggggtg taccatttct gatagaaatc tttaattttt
     9421 tccgcttcaa cgggttgttt cagcggatcg gcaggatcgt gacctaacaa ggttgaccct
     9481 ttcagacgat agcgccacca gccctcttta gtatcggcag gccaggttgc caccatgtcc
     9541 tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg tcaatttgcc agtggcattt
     9601 gccagataag agagcgcttc tttcagcaaa tcgttacggt tattgggcaa actcagatta
     9661 aacagcgtgg tgtcataaga gacaattacc ggcggcatcg ggcgtttagg gtcgatcccc
     9721 tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac cgctttgcgt tagcgcaata
     9781 cgagggatgg cgtgactgta accgctctgt tgtgtacttt cggcgagcga accggtatta
     9841 accagcaggc gaatttcaac acgatcgctg ggacgctggg gggtggtcag cacttgccac
     9901 tgtaaaccgt tggaaagcgt cccctgttgc catgctggat caggctggag cgcatctgcc
     9961 tgcacatagc cagcagtggc catcatcagc aaaccgcccg ctaaaagtcg aatttttatg
    10021 ccctgcatgt gaacccctga tcaactatcc tggtaataaa aaaactgccc gttgtcggtc
    10081 agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa gaacatgaaa atgtcacgga
    10141 agaagtgaaa taaacctgaa ctcacccagg tggtaagttc aggcacaggg gtcaattatg
    10201 cgcaaacacc cgcactcggg gaagggagtg cgggcataag tggtgagatt aagaggataa
    10261 ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca tcgtccagtt ttttgtggtc
    10321 cagttctttc acccacttag caacgacaat ggtcgctacg ccgttaccga ccaggttagt
    10381 tagcgcacga gcttctgaca taaagcggtc gataccgagg atcagcgcca gacccgctac
    10441 cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg ataaagccac tacccgttac
    10501 ccctgccgcc cctttagaag aaagcagcaa cacgattaac agcgtgattt ggtggacgat
    10561 atccatctga ctgttagtgg cctgggcgat aaacaccgcc gccattgtca ggtatatcga
    10621 tgtgccatca aggttaaacg agtagcctgt cgggatgacc agccccacca ccgatttacg
    10681 gcagccgagt ttctccatct tgtcgagcat acgcggcagc gccgactcgg aagatgaagt
    10741 ccccagtaca atcagcagtt cttcacggat gtagcggata aatttgaaga tactgaaacc
    10801 agtcgcttta gcgattgaac ccaataccag caccacaaac aggatacagg taatgtagaa
    10861 acagataatc agctgcccca gttgcaccag tgtgccgacg ccgtatttac cgatggtaaa
    10921 cgccattgcc ccgaacgcac caataggtgc cagacgcatg atcatattga tgatgccgaa
    10981 gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt tggcctttgc tgcccagacg
    11041 gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc tgcagaatgt taccgctggc
    11101 aaatgcgcca atgacgctcg ccgggatgac atccataatg aaggcgacaa tgccctggtc
    11161 tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca agcgttgccg gatcgacgtt
    11221 cattccggca ccaggctgca cgacgttaac gatgataaga ccaataatca gcgcgatggt
    11281 actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta cgaccgaccg ccttcatgct
    11341 ttccatgccc gcaatgcccg ttacgacggt acaaaagatg acaggagcga tgatcatctt
    11401 aatgagctta acgaagccgt cgccaagcgg tttcatttgc tcgcctattt caggatagaa
    11461 atggccaagg agaataccaa tggctatcgc tgtcaggacc tgaaagtaaa ggcttttaaa
    11521 cagagaggtt ttcatagggt gtcctttagt aaaaccacag gtcttgtaag gttatggggg
    11581 gtacctgcgg ccttaaaata acacccagac aacatcacag aaatgtacct ggatcataat
    11641 tgaaacaaaa aggttaaaaa gtttgagctg gctcgcacaa accagcactt tttaaagttt
    11701 tgtaatcagt ttggggtagc tacttttctt ccaggtaact ctcttcgaag atttcaatag
    11761 ggagtgggcg agcaaaaagg aagccctggg caataccaac gcccgctttt gccagccagt
    11821 ctcgttgtgc ttcagtctcc acgccttcgg caatcatttg taagttcagg ctctgcgcca
    11881 gcatgataat tgcagcaatc atgctgctat cttccggcaa gccttcaaca aacattttgt
    11941 cgattttcag tacgtcgatt ggcaacgatt tcatatgctg cagctgacgc agccctgcgt
    12001 agcccatgcc gaaatcatcc agcgccaccc gaactccggc attgcgcagc ggacggagga
    12061 ttgccaccgc agcatgaggg tcgtcaatac gtcggctttc tgtcacttcc agaatcagtg
    12121 ttcccggctg aatgcgatag cgggttaaca gttccagcat atccgccacc atattcgggt
    12181 gcatcagttg cagcgcagag aggtttaccg acaagggcag cataatgccg cgctcttgcc
    12241 aggctgcaag caatcgacag gactcttcca gcacccagtg accgacggta accatcagcc
    12301 cacagcactc aatgcgatcg attaagccat ccggcaggtc ccaactgcca tccggttgct
    12361 ggatacgcag taacacttcc gcactgacca gtttaccgct ggtcatctcg acctgtggct
    12421 gtaaccaaat tgcaaactga tgattttcca gtgcattaag gatatcgctc tcttccgtca
    12481 accgcttctg ggctgcttcc atctgctgcg gatcaaagaa ctgaatctga ttcttgcctt
    12541 tatggcgagc ggtaaatgcc gcagaaatag cgcgactgta aagctgttcg gcggtgagat
    12601 cgccgtagaa catcgccacg ccaatgctac agtgcggacg gagttgaata cgttcaatcg
    12661 gcaggcgctc gctcatgata gtgagtactt gctgacccaa agtgattgcg tgccacggtt
    12721 cctgtacacc gttggcaatg acagcaaagt cataaccgct aatctgcgcg aggatcatac
    12781 gtggcgacag taccgatttg agtttttcca ccagcgtcag cagcagaatt tctcgttgcg
    12841 cctctttcag cacgcccgca gtatcacgca gggtttcaca ggtgatgatc atcagcgcgg
    12901 tggtttgttt acgcgcgaca acctgctcca gcatctccat cagcaaggct ttgttcggca
    12961 aatccgacac cgggaagcgc atcgcatttt cgttctgttc ttcataatgg cgctgcagca
    13021 attgctggtt gaggttgtaa ctgcgcacca acataccgat ttcatcgtcc tgatgcagac
    13081 gcggtaatgc cagttggtga ccaacaagct cctgggctgg gatggcgtta agttcgcgag
    13141 caatattgcg taacggatgc aaaatcaggc ggttaatgca ccagctgatg gcgacggtca
    13201 ggataagcga caaaagtaag taaatggtca ctaacgttga gagagtgctc atcacgaact
    13261 tatacatacg gaaggaatcc gcctgtagca ccagataggc tattggctgc ggatttgccg
    13321 gacgttcgag cgagtagacg cccagcgaga tttgaaccgg tagctcaaac aggcgagtaa
    13381 ccattaccgg caccggacgc tctggaataa aacttttacg cagcgcctgg aactggttag
    13441 gcagcactac atcggcacgg ctgaccacgc ctgccggttt aatgctggca agaatagctt
    13501 ccgcttcggg aatatcgcct ttcaaaatag ctgaagataa gggttcgcgg acagagcgag
    13561 caatgctttc cagttgcgta gccgtgttat agcgattctg ctggaccaga tggaacagca
    13621 aaacggtgca aaaaataaaa acgaacacca ggacaacggc tgccaccatt gccatctgct
    13681 tgattgttaa cgagcgactt acgcgcaaat tgtctccccc cggaaatccc agattgccgc
    13741 ccgagtatac ccgatcgcag cggcattaag agaggcgcta tctgaaaact taccagtcgg
    13801 cgtaaggtat cagcggctgc ggcggtaaat ccatgtcacc ctgccatccg gcggcggaat
    13861 aacgtacgta gagcaggaaa tggctgggtg cgtaatcttt cgcctgctgg atatcaattg
    13921 ccgtaccaac aaaccagttg gaagtaacac gtcgttcaag taatgcccgc gccgtgtagc
    13981 cgaagccctg actgctgccg ccatcgttgg attgccgcgc agcttcttcc tgccagtcgg
    14041 tcgggatcag attcatcagc ggataacgcg gcatggtttt ggtgcgtgaa tgcgaccacg
    14101 agccagacgc ccccagctcc cacgaccagt tttccgtgcg ctcccgccac atcaccggta
    14161 tggcaaacga caggtattcc tgcggactgt agtaaccgcc ctgaccgagt gagtagccac
    14221 tcagatcttt gtcgtaatgc cagatcatgt tgttcaggcc gattgtgacg cggcgattgt
    14281 tctggttaat gaccttatag taatagcccg tcatccagcg cacgcgccag ttatcttcga
    14341 catttttacc ggttaactgg tcgccactaa gcgatgccca gacgccgttt acttcacctt
    14401 tatcgtagct cagacttagc cccacaccgt cggcacgtac gccaccccat tttttcccgg
    14461 tattgctcgg ggagtctttt tgcccaccaa aggccagcaa agaactggag atgggccgac
    14521 ggtgggcgtt aacggtgtaa cccagcggcc cgatatcatc gctgtaactg atgccgccga
    14581 ccacatccac cacgttgaag cccatcggcg tggtaccgat atcccagctc cagacgtcat
    14641 ttcgccagcc gaccgccacg ctggcaccgg aatccgactg gctgcggttg ccgctacagt
    14701 cctgtaatgt acaggtgccc cagttgtcat cccatttgcc atcggcatta gtggagaaac
    14761 tgccgacgtt catattgacg aaatcactgc gaaagaacat ccgcccgtca gaatacggcg
    14821 catccacctg caacatggta gtgtgcgctt tcagatcgga gtaaccaccg gtgccgctcg
    14881 aaccccagta atcgtgctca agggtgacgt taagatcctg ctggcgatag aggtccgccg
    14941 catcgctgcg cacgccgcgt ttcagccagt catctttctc gtcgttacgg gtcagtcggg
    15001 taaaggtgtc gttatcctgc ggacgcgtcg tagtcacacc ggatgcgacc atggcgtctt
    15061 tgtaggtttc cagcgcctgc tttggatcgc ccgcctgcgc ttcaaacttc gcaccgtcgc
    15121 gcagtaccat cgcgctttcc atcgacggtg gctgagattt tgcctgcggg atcaacttat
    15181 taaacgtctg ctgcgccgct gcggtatctc caagctgcgc ctgtgccagc gccacgcgcc
    15241 gctgagtgtt cagcgaggtg ttatcggtgg caggcagttt cgccagctgg ctacgtgccg
    15301 ccgctttgtc accggcagca atatccactt ccgtcagacc aagaatggca tcggcgttag
    15361 ttggctcccg cgtcaggaca ttctgatatg cagtgcgggc ggcggtgtaa tcacgtcgtt
    15421 gttacgccca gtcagccagc gtgaggtcaa tacgcgtgga aggtggttgc tgacgcagca
    15481 tcgcttccgc ttcagcctct ttgccgcttt ctcgcaggcg attagcggtt tccagcacct
    15541 gatcgctttg cagtcgatta accagctcct gaatattgct gttccactgc gcgcgcggca
    15601 ggctgttgat atgcgccagc gccgctctgt cctggttgtg acctgagagg tacagcccgt
    15661 aagcgtaaac ctgctccggg ttgttcggct tctgctgcgc cagattgcgc attaacgtat
    15721 cggcctggct gcgttgtccg gcctgccaga gatcctgcga aagtcggtaa gtaatccata
    15781 cgctgccggg gcccagcgcc agtcgttgcc gctgaagtgc tgccgcctgc gcccatttgc
    15841 cctggttttc cagtacctct gcctgctgtg ccagacggtc gttttgcagg ctgcgttcga
    15901 tatcatcaat gctgcgccgc tggctggcag agagcgaggc gataaacgct tcagcttttt
    15961 ctggcgactg ctggcggtaa atattcgcca gcccgcgcac ggcattagtg ttgccgctgt
    16021 ccatccgcag ggtctgctga tggttggtgc tgcctcgacc atcggcatga cggccagacc
    16081 gagggaaagc gtgaatatgt ttagtgtgaa tttgcgcatt cctggcccca gtcaggtaat
    16141 aactcacctt ttgtcgagaa gcggaaacgg tgttgatccc agccttgtcc aaacagggtc
    16201 agcacatagt tgtaataggt atcgctgccg ggaaagttat cggccacgcg ctggcgctga
    16261 actgcctgcg catcgcggtt ttgtaaaaaa ggcagcatgg cggcagaaaa accgaccggt
    16321 cctttaccct gcgctttccc cgtagccaca tccacttttt ccggcggata accgtttttc
    16381 tcagtgaatg tcgccatcgg tttaaaccgg ttgagcatcc gcgctttttg cggatcgctg
    16441 tcaggcatca tgcctaccca catgtaaacg cggatagcgt cgtagctgct gatcaatgtt
    16501 ttttcggctt ttagctgcca gcctttgtct ttctcatagc gcacccagtc tggcgaaaag
    16561 cctttcgggg cggtttccag caataaatgt tgattggttt cgcgcagcgt ggtccacggc
    16621 gcgccaaagc gggtgaaata ctgcgccagc gtcggcggca tgtagctggg gttaaaacgc
    16681 cagctgttat cctcagcaaa acccactttg cccggtaaca acatggaacc cagcccaggc
    16741 accgtcacca cttcctcccg cgcgatacgt tttagcaacg cgctgccgat gtcggtataa
    16801 cgctgctctt tccacaaacg ccccgcctcc agcaacgacc aggccatcca gacatcacca
    16861 tcggaggccg aattgctgtc cagcacttcc cacttactgt tctctttctt gccccacagc
    16921 caggcgggca aacgttcttt taaagaaccc tgagcgagat tgttctgcgt ccagtcgaga
    16981 atattatcga aagctgcacg gtcgttagcc gccagggcaa agaacatacc gtaactttgc
    17041 ccttcggagg tggtgatttt gcgcgcgtcg ctggggtcga tgacgcgccc ttcctgactg
    17101 atgtaatcct ttttaaactg ctcccaggca ggccaggtac aggctgcctg aacactaaag
    17161 gcagccaaca acagcatcgt cacgattcca ctacgcaaca cattcatctt caattactcg
    17221 ttatccgggt taagacgacg acgacgacta ataattcgca gcagacgcca cagtacccat
    17281 gccagcaata tcacactgat agtcgccagc accgccagca gaatcggatg gtttgccagc
    17341 gcataccaca agcgctcgaa ccacggcaga tgacctacgt aataaacgtc gccaacacgt
    17401 aggctgttga taccggactc gcggatcacc gcgaccgaac cgaacatggt ggcgcgtttg
    17461 ccgctatcgt tcaccgcatc gttaagcatt tcataaccgc gtgggctatc tgccaacagc
    17521 gcaatcacgc tgcgctggtc gttatacggc gactggaagc caatcaccgc cgccatcgca
    17581 ccggaagagg tcagagttga ctgggtttct gccgcgcgat cgctctcgtc cggcacaatg
    17641 ccggggaacg gggtctggcg catcggtgtt ttcacccagc tttcggtcgc ctgcaccaat
    17701 aggtcgatct gcttatcgtc tttcagtttg tccgggatac caccgatgat catgatgtcg
    17761 gcatctttgc cctgaatggt gctgccatca tcggtcaccg tcagattaat cgccgggaag
    17821 cccgtctgtg cgccgataaa accaacagta ttcagcaacg tttccatctg tgcttcgtta
    17881 ggcgctttcg gcatcacggt gatggtttgc gacagatccg ccatccggct gaatgggaag
    17941 cccgcgttag caaaggcgcg tagatccggc atcgggatga agtggtaata cttcgagaag
    18001 tcgatggtgg aatcgtcacc aatcaccaca tgattctgca ccggctggaa ggtaatacag
    18061 ttatctaccg aaccgcctgg catcgggttc atatactcaa agtcgaagcg cagctggttg
    18121 gtcgcgccca gtttcagcgc cggaatagag acatctgttt tgccatccag caaaccttgt
    18181 aataccggaa tacgcagcag cagacggttc gcctcctgtt tgctgctcag gttgaaggat
    18241 tgcaggaact ggttattcag gctgatatcc atccgcgaac tgtctttcac cggcggcatg
    18301 gtgtagcggt aattaatatc catatcaatg ccggtactgc gcatcaggta gagatccggc
    18361 ggcaggttta gcgaaacgtt aatcgctgct ggctcaaggc cgctggattg taactgttct
    18421 tcataggttt tcagttcgcc aaatgtgacc ggacgatcgg tacgtaccca gttcggcgca
    18481 tcgtacggct tacgcggtag cagcggtttc acttcattca ctaccacgct ttcaccacgg
    18541 aacagaatgt taccctgagc gatacctttc gctgcctgca acaggtcttt gtcgtcacga
    18601 ccaaacacca ccagcagttt gacgtaagga ttctgcggat ggttaatcat ctcaatcacc
    18661 ggggctttta ccgccggatg atcgcgcagg aagtccggcc gtttgtcgtt ggttgcaaag
    18721 acaatggcat tgcgatccgg caattggtta tagagcaccg ggaagttctg cccacgccag
    18781 ccagaacgcg aaccaaacca cgaggcgaca atggcagatg cttgttgcag cccaacatcc
    18841 ggcgcacccg caaagaccat cggcaaggtg ttagtacggt tatcgcgcgg gtcaaagaat
    18901 ggcaccggga agtgtgacag gtcattcttc acattcaggg tctgataggt cagatccagt
    18961 ccactgctcc gcccaacatc cagccaaagc gtggtgctgg ccgggttttc gcacacgtcc
    19021 tgataatggc cgacaaactc cagccgcaca cggttgaagt cggtaataaa cagtggatta
    19081 atgggcattt gcgccagcgt ttttttaccc aactgttctt tggtcactgg cagcacgccc
    19141 atcagctcat cattgagata aacctttaac tgcgactgga caggcagtaa cgatggcgat
    19201 ggcgtgtatt cgaagttgag catcgccttc gtcaccactt catcgctgcg cataccaaac
    19261 tcaatgctgc cgttcgggtt aataccacgt agcaccatgc tgcccggcgg cggcgcgatt
    19321 tgcgcaaagg tcagcttcac atcacgcgaa ggaccgttct gcgccacgat gggggcatca
    19381 gctccctgct cgcctgacat cacttgcccc acctgcggat tttgttccgt ctgggcggct
    19441 acagctggct cagcattgat cagtggttgc gttgctggcg tcgcctgcgt catgaaagag
    19501 gggaacgcac tcatccccat agccactgca caaatccaga atagttttct tttcatcgcg
    19561 ttatcatcat tgttgagcca aagcctgatc cgatggttgt gccgtttcgc tccgctccgg
    19621 gcggcgcgga ataaacgata caacccagga aaccagagaa gtcagcacac ggaatatgcc
    19681 cttcaccgaa gaaggcgcaa actccgccag atggcggtag ccacggaagc cgagcttcag
    19741 aatatccagc agactttcca gcggcttatc ttccgggtag ctgtcctgcc agagcgccca
    19801 tgtatccgca cgggcaaacg tacactgcac aaaatcgata tgttgctggg tggtgagcgg
    19861 cattaatttc agcccaactt cattacccat cacgcgcgcc acctgggtcg ggaagacgta
    19921 ttcctgctga ccgcgtttaa gcaacagatt cactttctgc ccttccagaa tctgcgcctg
    19981 accgttgatc ttgatcccca aaccaccgtc ggagaaatcc tgaacggtac acgagaagag
    20041 gtgaccatct tcgcgggcaa ttgccgcggg catcgtcatc tccacgcggt tcgatcggcg
    20101 tacctgtttg ctttctaccg ataccgcaac tgcgccgcca agaacaatca ggttgtagaa
    20161 cacccacacc atactgacga ccacggtgag catctcggtt ggcgggccat agaagtagcg
    20221 ccagatgcct accgcaacgc ccaccaggtt gagtagaaca aggaagatgt agggccgcga
    20281 gatcacccag tcgacgtact cttcttccac cagtccacct ttggcggtga cgttaaattt
    20341 gcctttgtgc gggttaatca gcgccaccag cgtcggtggt gcgatatacc acgccagcac
    20401 cgtttcgtag atttcactcc agaaagagtg gcgatatttg ccctggatct tcgagttggt
    20461 caggctggca tggatcatat gcggcagcac gaatagggcg atcatcaacg ctggcgcata
    20521 gatgatgtag gcatgaagca gcaggaacgc cagcggcgca gtcaggaaga tcagccgtgg
    20581 aatgcccgac aagaagtgga acatggcgtt gacgtagcac agccgctgag caaacttcag
    20641 tcctttaccg gtgagcgggt tatcgagacg gaagatttat accatcccgc gcgcccagcg
    20701 aatacgctga ccgatatgcg ccgacagact ttcggtcgcc agccccgccg cctgcggaat
    20761 acgcatatac gcggaggtat agccacgacg gtgcaaacgc agagaagtat gcgcatcttc
    20821 agtcacggtt tcgacagcaa tgccgccaat ttcatccagc ggcttacgac gaatcaccgc
    20881 acaggaaccg cagaagaaag tggcgtccca catatcgttg ccatcctgca ccagaccata
    20941 gaacagcgtg ccttcgttcg gcgttttacg aaaacgtccc aggttgcgtt caaacgggtc
    21001 cggcgagaag aagtggtgtg gagtctgcat catcgccagc tgtttctctt tcaggaacca
    21061 gcccatggtc atttgcagga acgatcgcgt tggtacgtgg tcgcagtcga aaatcgacac
    21121 gaactcgcct ttggcatatt tcagcgcatt gttgatgttg cccgctttcg catgttcatg
    21181 agtggtgcgg gcgatatatt tcacccccac gttttgcgca aactggcgaa actcttccct
    21241 gccgccgtca tcgaggatcc agatgttcag tttgtctttc ggccagtcga tacccagcga
    21301 ggcgtaaatg gtatttttca ccacattgag atcttcgttg taagtcggga caaagatatc
    21361 cactgacggc cacagcgaca tatccttcgg caatggcacc ggctgacgat tcagcggcca
    21421 tactacctgg aagtagccga gcaccagcac aatccacgcg tacgtttcag cgaagagcag
    21481 aataagcccg cacaccaggc tgaccggatc gtcccagttc agcgtagagg tgtaacgcca
    21541 ccagatataa cggcaagaaa cggtcagcga cagcacaatc aacattagcg ccgagaagcg
    21601 ccccggcatc cgccgtacga tcagcgctac cccccacagc agcatcagga agataaactg
    21661 cgccagcggg ttaaacggct gagtaacgca gattaacgcc agaatcagcg agaaggtgac
    21721 gatgataccg aggatcaacc gccgcgcccc ggc
//
LOCUS       DAJVPP010000068        21280 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVPP010000068 DAJVPP010000000
VERSION     DAJVPP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.068"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(26..2095)
                     /gene="glyS"
                     /locus_tag="OQM34_002592"
     CDS             complement(26..2095)
                     /gene="glyS"
                     /locus_tag="OQM34_002592"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1452981.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGIIV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2105..3016)
                     /gene="glyQ"
                     /locus_tag="OQM34_002593"
     CDS             complement(2105..3016)
                     /gene="glyQ"
                     /locus_tag="OQM34_002593"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1452982.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3111..3410)
                     /locus_tag="OQM34_002594"
     CDS             complement(3111..3410)
                     /locus_tag="OQM34_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1452983.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3585..4580
                     /gene="wecH"
                     /locus_tag="OQM34_002595"
     CDS             3585..4580
                     /gene="wecH"
                     /locus_tag="OQM34_002595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1452984.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4622..5059)
                     /locus_tag="OQM34_002596"
     CDS             complement(4622..5059)
                     /locus_tag="OQM34_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCS1452985.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5105..5446)
                     /locus_tag="OQM34_002597"
     CDS             complement(5105..5446)
                     /locus_tag="OQM34_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1452986.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5615..7069)
                     /gene="xylB"
                     /locus_tag="OQM34_002598"
     CDS             complement(5615..7069)
                     /gene="xylB"
                     /locus_tag="OQM34_002598"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1452987.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7141..8463)
                     /gene="xylA"
                     /locus_tag="OQM34_002599"
                     /pseudo
     CDS             complement(7141..8463)
                     /gene="xylA"
                     /locus_tag="OQM34_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8829..9821
                     /gene="xylF"
                     /locus_tag="OQM34_002600"
                     /pseudo
     CDS             8829..9821
                     /gene="xylF"
                     /locus_tag="OQM34_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
     gene            9899..11440
                     /gene="xylG"
                     /locus_tag="OQM34_002601"
     CDS             9899..11440
                     /gene="xylG"
                     /locus_tag="OQM34_002601"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCS1452988.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11418..12599
                     /gene="xylH"
                     /locus_tag="OQM34_002602"
     CDS             11418..12599
                     /gene="xylH"
                     /locus_tag="OQM34_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCS1452989.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSTRNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12677..13207
                     /locus_tag="OQM34_002603"
     CDS             12677..13207
                     /locus_tag="OQM34_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1452990.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13319..13855
                     /locus_tag="OQM34_002604"
     CDS             13319..13855
                     /locus_tag="OQM34_002604"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1452991.1"
                     /translation="MIGIDNEELTRYLSRIALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPDVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13963..14787)
                     /locus_tag="OQM34_002605"
     CDS             complement(13963..14787)
                     /locus_tag="OQM34_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCS1452992.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15107..17137
                     /gene="malS"
                     /locus_tag="OQM34_002606"
     CDS             15107..17137
                     /gene="malS"
                     /locus_tag="OQM34_002606"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1452993.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17315..18550
                     /gene="avtA"
                     /locus_tag="OQM34_002607"
     CDS             17315..18550
                     /gene="avtA"
                     /locus_tag="OQM34_002607"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCS1452994.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDDALANQHGIPLVIDNAYGVP
                     FPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGIISLAPG
                     GIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHKPEGAIF
                     LWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYVPEPEKI
                     EAGVKILAEEIERAWAESH"
     gene            complement(18701..19174)
                     /locus_tag="OQM34_002608"
     CDS             complement(18701..19174)
                     /locus_tag="OQM34_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1452995.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19276..20124)
                     /gene="yiaJ"
                     /locus_tag="OQM34_002609"
     CDS             complement(19276..20124)
                     /gene="yiaJ"
                     /locus_tag="OQM34_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCS1452996.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20325..>21280
                     /gene="yiaK"
                     /locus_tag="OQM34_002610"
     CDS             20325..>21280
                     /gene="yiaK"
                     /locus_tag="OQM34_002610"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCS1452997.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITV"
BASE COUNT         5352 a         5433 c         5216 g         5279 t
ORIGIN      
        1 ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca acgcgcagga
       61 acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct ttgtcatcaa
      121 ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct gccagttcga
      181 ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca cgtagcacca
      241 caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca ttcacgcgat
      301 cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc gccgccgcca
      361 gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg gcatcgaaat
      421 cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg gtataacctt
      481 cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca tcaactacgt
      541 tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc gtttgcagat
      601 caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca cgacgcagcg
      661 caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc gccagagtat
      721 ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg tcatcaccgg
      781 caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct tcgccatcgt
      841 gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag accatgttgg
      901 tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg tcagcgccaa
      961 tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca cgcagcgtac
     1021 ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc agacgttttt
     1081 tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg actttctcgt
     1141 taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag ataaagttcg
     1201 gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct ttcatggtgt
     1261 aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg gtcagaacga
     1321 ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag tcagcgttac
     1381 cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta cgttcttcgt
     1441 aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg gcgttgtcga
     1501 tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg gactgaatgc
     1561 ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg tgcaccggac
     1621 gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc agtttcgcca
     1681 gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc acatgggcgc
     1741 gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca acgataatac
     1801 cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg tcgaacgcct
     1861 gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc gccaggttag
     1921 ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg tgtgcgaggc
     1981 ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg ctgcgcagtg
     2041 cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca gacatagccg
     2101 cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa gcgtaatatg
     2161 cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga cgctcggtga
     2221 cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc agaatacgct
     2281 cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct tctttctcgt
     2341 actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg taagtggact
     2401 gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc gggccgtcgc
     2461 tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga cgttccagac
     2521 cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc tggaagtaag
     2581 tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc caggccccca
     2641 gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc gggtccatgc
     2701 ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat ggcttaatga
     2761 ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag cgaccatcgg
     2821 tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc agcgcgcgca
     2881 gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt tgaacaatgg
     2941 tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg aaggtcctgg
     3001 tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg aagggatcag
     3061 tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc ttaacgcatg
     3121 gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc tttacggaag
     3181 gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac gtcgattttt
     3241 tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc catattcaac
     3301 gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg tgtagaacaa
     3361 cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat taaattaccg
     3421 tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat cttacgaatt
     3481 tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc gccaaccaaa
     3541 tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag cccaaaattt
     3601 actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt cacaccacta
     3661 cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc gccaatgttc
     3721 tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc tatctctttt
     3781 ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt ctgatttttt
     3841 atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg gagttagcgc
     3901 tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc gcgattgcgg
     3961 tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa atgttgctgg
     4021 tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag aaaattgacg
     4081 gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac tacattctgt
     4141 atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca ctgtcgtggg
     4201 tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca ttatatgaat
     4261 tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg atggttttta
     4321 tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt accattcgcg
     4381 gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc ttgattatcc
     4441 atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat attatttgga
     4501 tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa cgaatcgaca
     4561 gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg gcttacgtga
     4621 attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt attccggcat
     4681 cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa gccagtccat
     4741 aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc agtagagcaa
     4801 cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc ggtatgcctt
     4861 catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct aacaccagta
     4921 cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc cctaacaggt
     4981 aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc ggtgaatagg
     5041 ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa gagctttatg
     5101 ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa aaggcgatgg
     5161 ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata aacaagagac
     5221 cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc agttgcccga
     5281 gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac acgccaagaa
     5341 aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga taaaccagcg
     5401 ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg atctccatat
     5461 ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg ccattaacaa
     5521 atgattttca gaataaattc atactaaata ttaattaatt agtgagatat atagatgtga
     5581 attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc agaagttgct
     5641 gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc tgcgcatctg
     5701 gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat ttctctggat
     5761 tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc ccccccgtac
     5821 ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac tcactacgcg
     5881 ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc acgacatcca
     5941 tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt tcattggggc
     6001 catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg tgtggtgtac
     6061 gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca gcctgttgag
     6121 ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc caatccagac
     6181 acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc gcatggcaaa
     6241 agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca aaatagaccc
     6301 ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca ccaactgcac
     6361 cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc cacgctttcg
     6421 caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg ggcatctggt
     6481 cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt gcgacatcca
     6541 gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc atacgcagac
     6601 gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc ggctcatgcc
     6661 gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg ccggtaatca
     6721 ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag cgcccgtcgt
     6781 tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt gctccatgca
     6841 tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg cccagagctt
     6901 tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac caaagtggat
     6961 gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc tgctcgttga
     7021 gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata tcgatcgttc
     7081 cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca cagttagccg
     7141 ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc gaccactctg
     7201 atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca gtgacatttg
     7261 gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct gcgcgatgcg
     7321 tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca gtgccatcgt
     7381 atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt gacgacgtac
     7441 tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa tttcatacat
     7501 caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc ccagttgcgc
     7561 atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg tggctatttc
     7621 atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca gtttaatctc
     7681 tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt aatcatattg
     7741 atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga agccgatttt
     7801 atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct cctgacgcaa
     7861 gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat agttttcacc
     7921 gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc agctgaagac
     7981 ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa agcagttagc
     8041 ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga catcaaccat
     8101 ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa catccacatc
     8161 gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga catctgcttt
     8221 acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa acgcccccac
     8281 accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg caaaacgcaa
     8341 gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac ggaatgctaa
     8401 cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa aataggcttg
     8461 catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt aatattgggc
     8521 actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa ttcacaagtg
     8581 tgcgctcgct cgcaaaataa aatgggatga tgaaactggg taattcctcg aagataaaaa
     8641 tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa gaaataaacc
     8701 aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag ttgctaaaaa
     8761 ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac tacagaaggc
     8821 cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt ctgcttacca
     8881 acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat ctccgtcttg
     8941 aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc ggtgcgaaag
     9001 tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt gaaaacatga
     9061 ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta ttaagtaacg
     9121 ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt atgattaacg
     9181 atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa ctgcaggcaa
     9241 aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc tcgccggtag
     9301 ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct tacgttgatt
     9361 ctggaaaaat taaagtcgtt ggtgaccaat gagttgatgg ctggttaccg gaaaacgcat
     9421 tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct gtagttgcct
     9481 caaacgatgc caccgcagga ggggcaattc aggcattaag cgcgcaaggt ttatcaggga
     9541 aagtagcaat ttccggctag gatgcggatc tcgcaggtat taaacgtatt gctgccggta
     9601 cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc gcagaaattg
     9661 ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat aatggcctga
     9721 aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac atcaaagata
     9781 cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc cccggcgcgg
     9841 agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg gagtcgttat
     9901 gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga aggcgattga
     9961 taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg aaaatgggtc
    10021 tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct cctacgaagg
    10081 cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata ccgaacgcaa
    10141 aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg tgctggaaaa
    10201 tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc tgatgacgct
    10261 acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata cccgcgttgg
    10321 cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta ataaacaggt
    10381 gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa cgtcggtttt
    10441 actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata tttcgcacaa
    10501 actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg gacagcacat
    10561 tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga tggtcgggcg
    10621 agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa tattacgtat
    10681 tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta atgatgtctc
    10741 gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg ccggacgtac
    10801 cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa aaatttatat
    10861 tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcgcccagg ggattgcgat
    10921 ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg gtaaaaatat
    10981 taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg acgcggcaga
    11041 gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt cccccgacct
    11101 tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc gctgtctgtt
    11161 acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata ttggcgcgaa
    11221 atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg ttattgtcat
    11281 ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga tgcatgaagg
    11341 gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga tggaagccgc
    11401 attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg gccgtaccga
    11461 catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg atgattgcag
    11521 ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac ttaagcaccc
    11581 gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg gtaggaatgg
    11641 tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg gggctattag
    11701 gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt accatcattg
    11761 tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc gcgtaccgca
    11821 aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc atactcattg
    11881 gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa attgggcaaa
    11941 gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg gcttttgttg
    12001 gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct ccggcctcta
    12061 ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca atctggctgt
    12121 tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg ttactcggcg
    12181 gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc ggcggcaatc
    12241 tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc gtgttcgcga
    12301 ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt ggcgctggtt
    12361 caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg attggcggca
    12421 ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca tttatcatgg
    12481 cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag tatatcgtta
    12541 aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc cgttcttgat
    12601 tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt ttgcatgatt
    12661 cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt tcaatgccaa
    12721 taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg catcacaatc
    12781 agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa tcaaggactg
    12841 gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag cgctggctga
    12901 tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa gttacccacc
    12961 cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt tgcatttaaa
    13021 agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg gcaaacgttg
    13081 ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt atcgcggagt
    13141 ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa atcggctggc
    13201 agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg acgcccgagc
    13261 gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa aattatgcgt
    13321 gattggcatc gataacgaag aactgacccg ctatctgtcg cgtatcgccc tttcttcggt
    13381 cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc gattattaga
    13441 taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca ttgaacggcg
    13501 ctcaacagat tatcgctcgc tgaccgatcc cgacgttatt caggccatgc attacattcg
    13561 taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg ggatctcgcg
    13621 ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg caatgattca
    13681 tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt cgatcaatga
    13741 gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt ttaaaaaagc
    13801 atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt tgtagcgatg
    13861 atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc gagcaaaagc
    13921 cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat gcgccgcgat
    13981 taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct tccctttggt
    14041 cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat ccgctttacg
    14101 cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca ggttagtgac
    14161 ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca ctttacccgg
    14221 cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat tgttgcgcgc
    14281 cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca tactggtagg
    14341 gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat taccagacca
    14401 cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag gtgaccattg
    14461 gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg cattttggct
    14521 ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc cggaagggta
    14581 ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact cttgtttact
    14641 gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg ttgttgtgtg
    14701 cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa tcatcgcccc
    14761 atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat cattccaaca
    14821 ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa tagctgtcaa
    14881 gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga ttcagattgg
    14941 cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt gcgcgcgatc
    15001 gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt gtctggctta
    15061 tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga aactcgccgc
    15121 ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt ctccggggtt
    15181 ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt tgcccaaagg
    15241 tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag atgcgataaa
    15301 actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat ggcgattgtt
    15361 cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa cattgatgat
    15421 ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc cgaaatggga
    15481 tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg ccgtacggga
    15541 ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac aacccgctgc
    15601 taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg cccctttcga
    15661 ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg gcgatcccag
    15721 taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca cttttcacgg
    15781 cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg gcgttaatgc
    15841 tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg gtacaaaagg
    15901 cgatttcccg cattatgcct accacggtta ttacacacag gactggacga atcttgatgc
    15961 caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc agcgcggtat
    16021 tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg cggatatgca
    16081 ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa cgctgggtga
    16141 acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta acgattacat
    16201 taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga tcagaacgga
    16261 tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg cctttttgcc
    16321 ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata aaaacaaaac
    16381 ggatactcac gctaaagtca tcgaaggctt tacgcctcgc gattacttaa cccactggtt
    16441 aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg ccaaacatgt
    16501 tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc gcgaatggaa
    16561 aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg gtgaagcctg
    16621 gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga tgatcaattt
    16681 cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg atacgacctg
    16741 gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct cgtcgcatga
    16801 tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat tagcgccagg
    16861 cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta caggttctga
    16921 tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta aatctgccgc
    16981 caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg caattggcgc
    17041 gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg agcatggcga
    17101 cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc ccgttcgtca
    17161 gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt tgcgggataa
    17221 agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg ttattgttac
    17281 cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt ttggtgacaa
    17341 atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg gtttacgcac
    17401 gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa tgcaggacta
    17461 cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg cactgtgtaa
    17521 ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa tgctgcgcga
    17581 gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca gccagagcgc
    17641 gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg tcaaaaaagt
    17701 gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg aagaagatct
    17761 gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta aataccacgt
    17821 cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct cccggccgac
    17881 gaatccaaca ggcaatgtga ttactgatga cgcgctggcg aatcaacacg gcattccgct
    17941 ggtgattgat aacgcttatg gcgtcccgtt cccgggtatc atcttcagtg aagggcgccc
    18001 gctatggaat ccgaatatcg tactgtgcat gagtctttcc aagctggggc tgcctggctc
    18061 ccgctgcggc attatcatcg ccaatgaaaa aatcatcact gccatcacca atatgaacgg
    18121 cattatcagc ctggcacctg gcggtattgg tccggcgatg atgtgtgaaa tgattaagcg
    18181 taacgatctg ctgcgcctgt ctgaaacagt catcaaaccg ttttactacc agcgtgttca
    18241 ggaaactatc gccatcattc gccgctattt accggaaaat cgctgcctga ttcataaacc
    18301 ggaaggagcc attttcctct ggctatggtt taaggatttg cccattacga ccgagcagct
    18361 ctatcagcgc ctgaaagcac gcggcgtgct gatggtgccg gggcacaact tcttcccggg
    18421 actggataaa ccgtggccgc atacgcatca atgtatgcgc atgaactacg taccagagcc
    18481 ggagaaaatt gaggcggggg tgaagattct ggcggaagag atagaaagag cctgggctga
    18541 aagtcactaa aaattgcctg atgcgctacg cttatcaggc ctacatgatt tctgcaatat
    18601 attgaatttg gaagaatttg taggccggat aaggcgttta cgccgcatct ggcataaaca
    18661 aagcgcacta ttccggcgtt gagaaacgcc ggaaaacgtt tcaaacaggc tgctgctgta
    18721 gccgatgccg ctgcactttc tcgacatcca tgcactgcaa cgcctttgtc gggcaggctt
    18781 caacacaggc cggccccgtc tcccgatgcc agcacagatc gcacttaatc gccctcgcct
    18841 ttttgcgcga agaaacgacc tccatcgcac caaacgggca agccagcata cagcttttac
    18901 agccaatgca acgtgtttgt tcaacgaaaa tatgcccatg ttcgcggctt atcgcgtcaa
    18961 cagggcagac attcgcgcac ggtgcatctt cacactgatg acaggctact ggcgtggtcc
    19021 agctgtggtc tttaatgaca cgaatacggg aaataaactc gtctggtgac aacgcagcgc
    19081 aatcctgatt ctcctgatgc gacactgcgc aagccacttc acaggtacgg caaccgatac
    19141 atttcgtcgc atccgcaata ataaaccggt tcatgatcat tcctttgcac taaaacagca
    19201 gagtgccaga gagcgcgtcc ctactgcttt gatccaacga aggaaatgtt aaagttttgg
    19261 cacttgtcca aagcgttatg tgatcgcgcc caggtcatcc ctgacagtaa atcccagttc
    19321 attagaaata gcctgcgcgg tttcacgcag tggtttcagg agatttttct ctcccacctg
    19381 tttcagacgt gatgtcgaaa gcgaaatcga cacggcgtac ggcacccgcc catgaatatc
    19441 aaacaccgga acagcaatac aggagacgcc gagttcgttt tcttctctgt ccatcgccgc
    19501 tccgctttca cgaatgtgcg ccagttcgtc gaacatcgcg ggcagctcgg taatggtatt
    19561 gcgggttaac ggctggatct catgctgatg gttttcccag tatgacttca cgtagtccgg
    19621 gtgaccaaac gccatgtaga tcttgcccat tgcggaacag tagagcggca tatgctggcc
    19681 aatataggca cgggttcgca gcatcccggt tgtgggttcc agcttataaa tcaaaatagc
    19741 gtgatcgtct tcgcggctgg agaagttaat ggtttcacca gtggcgatgt tcagtgcctc
    19801 aagatgcgga gcggcgatat gaatgatatt cagcgaagac agcgccttct gcccgacggc
    19861 aataaatttg gtagtcaggc ggtaactccc tgcggcgggc gcggtggtca catagccgca
    19921 ggactgtaat ccctgcaata agcgatggac ggtactctta tttaaaccag ccagctccga
    19981 aagatgcgcc aacggacaac cgtttggata gttgctcaaa atctcaatca gcatcaaccc
    20041 gcgaaacaga ctctggcttc cggctggacg ctctttttcc tgcgccatct cgttctcttt
    20101 ttttcccatc acttctttcc ccattttgtc gcgtcctgat ggtagcgcaa agtgtgccgt
    20161 agttcacgat ctcgacagat aatttataac cacttgattt ttatgtcttt tgaaattcat
    20221 caatcagatt gctttgttaa aaagtgatcg atatatttga aatctagttc cgcatattga
    20281 aattttaagc caaaaaagcg atcaaaaaaa caaggaagcc taggatgaaa gtgacatttg
    20341 agcagttaaa agcagccttt aatcgggtct taatttcacg cggcgttgac agcgaaacgg
    20401 ctgacgcctg tgcagagatg ttcgcccgca ccaccgaatc cggcgtttat tctcacggcg
    20461 ttaatcgttt ccctcgtttc attcaacaac tggaaaacgg cgatatcatt cctgatgccc
    20521 aacccaaacg tataaccagc ctcggcgcaa ttgaacagtg ggatgcccag cgttcgatcg
    20581 gcaacctgac ggcgaaaaag atgatggatc gcgccattga actggctgcc gatcacggta
    20641 ttggtctggt ggcacttcgt aatgccaacc actggatgcg cggcggcagc tacggctggc
    20701 aggcggcgga aaaaggctat attggcattt gctggaccaa ctccatcgcc gtaatgccgc
    20761 cgtggggcgc aaaagagtgt cgcatcggca ctaacccgct gatcgtcgcc attccttcca
    20821 cgccgatcac catggtcgat atgtcgatgt tctcttacgg catgttagaa gttaaccgcc
    20881 tggcaggccg tcagctcccg gtcgatggtg gctttgatga tgagggcaat ttgaccaaag
    20941 aacctggcgt tatcgagaag aatcgccgca ttttgccgat gggctactgg aaaggttctg
    21001 gcatgtcgat tgtgctggat atgatcgcta ctctcctttc cgacggcgca tcggttgccg
    21061 aagtcaccca ggacaacagc gacgaatacg gcatttcaca aatttttatt gccattgaag
    21121 tggacaagct tatcgacggt cccacccgcg atgccaagct gcaacgtatc atggattacg
    21181 ttactaccgc tgaacgcgct gacgaaaacc aggccatccg cttacccggc cacgaattta
    21241 ctaccctgct ggccgaaaac cgccgtaacg gcatcactgt
//
LOCUS       DAJVPP010000069        21017 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVPP010000069 DAJVPP010000000
VERSION     DAJVPP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.069"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..866)
                     /gene="fbp"
                     /locus_tag="OQM34_002611"
     CDS             complement(<1..866)
                     /gene="fbp"
                     /locus_tag="OQM34_002611"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1452998.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTSVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGG"
     gene            1042..2415
                     /gene="mpl"
                     /locus_tag="OQM34_002612"
     CDS             1042..2415
                     /gene="mpl"
                     /locus_tag="OQM34_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1452999.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2498..3049)
                     /locus_tag="OQM34_002613"
     CDS             complement(2498..3049)
                     /locus_tag="OQM34_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1453000.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3143..4495
                     /gene="pmbA"
                     /locus_tag="OQM34_002614"
     CDS             3143..4495
                     /gene="pmbA"
                     /locus_tag="OQM34_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1453001.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSVSSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4678..5064
                     /gene="cybC"
                     /locus_tag="OQM34_002615"
     CDS             4678..5064
                     /gene="cybC"
                     /locus_tag="OQM34_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS1453002.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5109..5573)
                     /gene="nrdG"
                     /locus_tag="OQM34_002616"
     CDS             complement(5109..5573)
                     /gene="nrdG"
                     /locus_tag="OQM34_002616"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1453003.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5731..7869)
                     /gene="nrdD"
                     /locus_tag="OQM34_002617"
     CDS             complement(5731..7869)
                     /gene="nrdD"
                     /locus_tag="OQM34_002617"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1453004.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSIFTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8263..9918)
                     /gene="treC"
                     /locus_tag="OQM34_002618"
     CDS             complement(8263..9918)
                     /gene="treC"
                     /locus_tag="OQM34_002618"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1453005.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(9968..11389)
                     /gene="treB"
                     /locus_tag="OQM34_002619"
     CDS             complement(9968..11389)
                     /gene="treB"
                     /locus_tag="OQM34_002619"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS1453006.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11508..12455)
                     /gene="treR"
                     /locus_tag="OQM34_002620"
     CDS             complement(11508..12455)
                     /gene="treR"
                     /locus_tag="OQM34_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1453007.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12640..12693
                     /gene="mgtL"
                     /locus_tag="OQM34_002621"
     CDS             12640..12693
                     /gene="mgtL"
                     /locus_tag="OQM34_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1453008.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12834..15530
                     /gene="mgtA"
                     /locus_tag="OQM34_002622"
     CDS             12834..15530
                     /gene="mgtA"
                     /locus_tag="OQM34_002622"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1453009.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15736..16122)
                     /gene="ridA"
                     /locus_tag="OQM34_002623"
     CDS             complement(15736..16122)
                     /gene="ridA"
                     /locus_tag="OQM34_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1453010.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16195..16656)
                     /gene="pyrI"
                     /locus_tag="OQM34_002624"
     CDS             complement(16195..16656)
                     /gene="pyrI"
                     /locus_tag="OQM34_002624"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1453011.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16669..17604)
                     /gene="pyrB"
                     /locus_tag="OQM34_002625"
     CDS             complement(16669..17604)
                     /gene="pyrB"
                     /locus_tag="OQM34_002625"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1453012.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17608..17742)
                     /gene="pyrL"
                     /locus_tag="OQM34_002626"
     CDS             complement(17608..17742)
                     /gene="pyrL"
                     /locus_tag="OQM34_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1453013.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18023..18418)
                     /locus_tag="OQM34_002627"
     CDS             complement(18023..18418)
                     /locus_tag="OQM34_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1453014.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18550..19263)
                     /gene="bdcA"
                     /locus_tag="OQM34_002628"
     CDS             complement(18550..19263)
                     /gene="bdcA"
                     /locus_tag="OQM34_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1453015.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19334..19927
                     /locus_tag="OQM34_002629"
     CDS             19334..19927
                     /locus_tag="OQM34_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1453016.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20072..20524
                     /gene="tabA"
                     /locus_tag="OQM34_002630"
     CDS             20072..20524
                     /gene="tabA"
                     /locus_tag="OQM34_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1453017.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20647..>21017
                     /locus_tag="OQM34_002631"
     CDS             20647..>21017
                     /locus_tag="OQM34_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1453018.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLA"
BASE COUNT         5037 a         5237 c         5549 g         5194 t
ORIGIN      
        1 ttaccgcccg cttgttccgc caggaatgcc atcgggttgc actcataaag caaacgcagt
       61 ttgccgtccg ggtggctggc ggtgcttggg tagagataaa taccgccttt cagcaggtta
      121 cggtggaaat ccgcaaccag tgaaccgata taacgcgagg tatacgggcg attggtggat
      181 ttatcttctt cctggcagaa tttaatgtac ttcttcaccc cgttcggaaa cttaatgtag
      241 tttccttcgt tgatggagta ggttttgcct ttctccggga agcgcatccg ttcctggcac
      301 aggcagaaaa cgcccagcga aggatcgtaa gtaaaggcgt gaacaccgca tccggtggtg
      361 taaaccagca tggtagagga gccgtatacc acgtaacctg ccgcaacctg tttgttacca
      421 ggctggagga aatcttcttc cgttaccgac gtgccaacag gcgtaacgcg gcggtagatg
      481 gagaaaatgg taccgacaga gacgttaaca tcgatgttgg acgagccatc cagggggtcc
      541 atcagcacca cgtattttgc gtgttcacag ccttcaaaga cgacaatctc atcttcttct
      601 tcagaggcaa tccccgcaac gatatcgcgt gctttcagtg cggctttcag tttttcatta
      661 gcgaacaagt cgagtttttg ctgaacctcg ccctgcacgt tctcagcacc gctggcaccc
      721 aggatatcaa ccagtcctgc tttgttgata tcgcgatgga taatcttggc gcccagtttt
      781 attgccgaca gcaaagcagt gagctcaccg gtagcatgag aaaactcgtg ctgcttttcg
      841 acaataaatt cacctaacgt tttcataaaa ctttccctgc aatgtttatg gagtaaagcg
      901 accgcaacaa tcttaacaaa taatctcaat gttgcgctca ggtgaatcgc gccagcaaat
      961 tgcggattat cctgaaatgc gtttctcact tgcccgacat atgcgtaaaa tgagcggcag
     1021 attaaaaaag gatagtgacg tatgcgcatt catattttag gaatttgtgg cacatttatg
     1081 ggcggtctgg cgatgctggc gcgccagtta ggccatgaag taacgggttc ggacgccaat
     1141 gtgtatccgc cgatgagcac cttacttgag aagcaaggca ttgagctgat tcagggttac
     1201 gatgccagcc agctcgagcc gcagccggat ctggtgatta ttggcaacgc catgacccgt
     1261 ggaaatccgt gtgtggaagc ggtactggaa aaaaacatcc cttatatgtc aggtccacag
     1321 tggctgcacg attttgtgct gcgcgaccgc tgggtgctgg ccgttgccgg tacacacggc
     1381 aaaaccacca ccgcgggaat ggcgacctgg attctggaac agtgtggtta caaaccggga
     1441 tttgtgatcg gcggtgtgcc ggggaacttt gaggtttcgg cgcgtctggg cgaaagcgac
     1501 ttctttgtta tcgaagcgga tgagtacgac tgcgccttct tcgacaaacg ctctaaattt
     1561 gtccattact gcccgcgtac gctgatcctc aacaaccttg agttcgatca cgccgatatc
     1621 tttgacgacc tgaaagcgat ccagaaacag ttccaccatc tggtgcgtat cgttccgggg
     1681 cagggccgta ttatctggcc ggaaaatgac atcaacctga aacagaccat ggcgatgggc
     1741 tgctggagcg agcaggagct ggtgggtgag cagggtcact ggcaggcgaa aaagctgacc
     1801 accgatgctt ccgaatggga agttttgctg gatggcgaaa aagtgggcga agtgaaatgg
     1861 tcgctggtag gcgaacataa tatgcacaat ggcctgatgg cgattgcagc ggctcgccat
     1921 gttggtgtag cgccggcaga tgccgctaac gcgctgggtt cgtttattaa tgctcgtcgc
     1981 cgtctggagt tgcgtggtga agcgaatggc gtcacggtat atgacgattt tgcccatcac
     2041 ccgacggcga ttctggcaac gctggcggcg ctgcgtggca aagttggtgg tacggcgcgc
     2101 attattgctg tgctggaacc gcgctcgaat accatgaaaa tggggatctg caaagacgat
     2161 ctggcacctt cattaggtcg tgccgatgaa gtcttcctgc tgcaaccggc gcatattccg
     2221 tggcaggtgg cagaagtggc agaagcctgc gttcagcctg cacactggag tggcgatgtg
     2281 gatacgctgg cagatatggt ggtgaaaacc gctcagcctg gcgaccatat tctggtgatg
     2341 agcaacggcg gttttggtgg gatccatcag aaactgctgg atggtctggc gaagaaggcg
     2401 gaagctgcgc agtaattcgg cttcagcctg atatagcatt gccggataag gcgtttacgc
     2461 cgcatccggc aaaccatact cacatcaaca acgaaaatta cccttcattc tccgccagtt
     2521 cgcgcagata ctggaaaatc tggcgtgcgg atttcggcgg cttattccct tctttctctt
     2581 tcttcgcgtt gcggatcagt gtacgcagtt gctgacgatc ggcatcgggc cacagattca
     2641 atacttcagc gatggcgtca tcaccttgat cgatcagacg atcgcgcagg ttttccagtt
     2701 tatggaacag caccacctgc tggttgtgac ggtttttcag cttatccagc gcctgacgaa
     2761 taggctctac gtcgcgctgg cgcagcatct taccgatgag ctgcaactgg cggcggcgac
     2821 cttccatctt aatacgctgg gcgagctcaa tagccgggcg cagatccgca tctaacggga
     2881 tcttatccag cgcgtttttc cccagatcaa caatttccgc gccaaggcgt tttagctcct
     2941 cggcatcacg tttaatttca cttttactga cccagataat ttcatcgtct tcgtcttcga
     3001 tgtcatcacc gggaacgtcg tcgagccagt cttcgggctg cttagtcatc tcaggctcct
     3061 taaaaaaaga ggctaatgtt accagttaag atgcgcactg aaaaacggtt ctctgttaga
     3121 cttcagagaa actctctaca ttatggcact tgcaatgaaa gtaatctctc aagttgaagc
     3181 gcagcgcaag attctggaag aagcagtttc cactgcgctg gagttggcct caggcaaatc
     3241 ggacggtgcg gaagttgccg tcagcaagac caccggcatt agcgtaagca cgcgttatgg
     3301 tgaagtggag aatgttgaat tcaatagtga tggcgcgctg gggatcactg tttatcacca
     3361 gaaccgtaaa ggtagcgtat cgtctaccga tttaagcccg caggccattg cccgcaccgt
     3421 acaggcggcg ctggatattg cccgttatac ctcgccagat ccctgtgccg gcgtggcaga
     3481 caaagagctg ctggcctttg acgcaccgaa tctcgacttg ttccaccctg cggaagtttc
     3541 cccggatgaa gccattgaac tggcggcccg cgcagaacag gcggcattgc aggcggacaa
     3601 acgcatcacc aataccgaag gtggcagctt taacagccac tacggtgtca aagtttttgg
     3661 caacagccac ggcatgttgc agggttactg ctcaacgcgt cattcgctct ccagctgtgt
     3721 aattgccgaa gaaaatggcg atatggagcg tgattacgcc tacaccattg gccgcgcgat
     3781 gagcgatctg caaacgccag agtgggtcgg ggccgactgt gctcgccgta ctttatcgcg
     3841 cctgtcaccg cgtaaactct ccaccatgaa agcgccggtc atttttgcca atgaagtggc
     3901 aaccgggctt tttggccatc tggtgggggc gatagcgggt ggctcggttt accgtaaatc
     3961 tacctttctg ctggattcgc tgggtaaaca aattctgccg gactggctga ccattgaaga
     4021 gcatccgcat ctgctgaaag ggctggcgtc gacgccattc gacagcgaag gtgtgcgcac
     4081 cgagcgtcgc gatattatta aagatggcat cctgactcag tggctgctga ccagctactc
     4141 ggcgcggaaa ctggggctga aaagcaccgg acatgcgggc ggtattcaca actggcggat
     4201 tgccggacaa gggctaagct tcgaacaaat gctcaaagag atgggcaccg ggctggtggt
     4261 gacggagctg atgggccagg gcgtgagtgc cattaccggt gattattccc gtggtgcagc
     4321 gggcttctgg gtagagaatg gcgaaattca gtatcccgtt agtgaaatca ccatcgcagg
     4381 taatttaaaa gatatgtggc gcaatattgt caccgtcggt aacgatattg aaacacgcag
     4441 taatatacag tgtggttctg tgctgttgcc ggagatgaaa atcgccgggc agtaaaaaac
     4501 tggcgcgacc tgccgcgcta taaactaaaa aattacacaa aatcattcgc actgcatcga
     4561 gacggaaagt gaatgagggc cggggagcgt actcgcagta cgtgacccgg gcgaatgagc
     4621 gtagccgtcg atgaggcagt gtgaaggatg aagtgtaaat aaaaaaggaa gtgagcaatg
     4681 cgtaaaagcc tgttagctat tcttgcagtc tcctcgttgg tattcagttc tgcgtcgttt
     4741 gccgctgatc ttgaagacaa tatggaaacc ctcaacgaca atttaaaagt gatcgaaaaa
     4801 gcggataacg cggcgcaagt caaagacgcg ttaacgaaga tgcgcgccgc agcgctggat
     4861 gcgcaaaaag caacgccgcc gaagctcgaa gataaatcac cggacagccc ggaaatgaaa
     4921 gatttccgcc acggtttcga cattctggtc ggtcagattg acgacgcgct gaagctggca
     4981 aatgaaggta aagtaaaaga agcgcaggct gctgcagagc aactgaaaac gacccgcaac
     5041 gcctatcacc agaagtatcg ttaattcctc atttccctgt tgcctgcact caggtaacag
     5101 ggaacttatc atcgcaaatg atgcaccacc tgattgctgc tgccgcgcca gatcagggaa
     5161 gggtctttta aatcctgcac aaatttgccg tcgaccagca cgttaatcaa atcaacaacc
     5221 tgcatttgcg cagcgttgag ttcgtcgagt ttatagcctg tccacaccca gatgtcttta
     5281 cccggacact cggcgcggat gcgttttacc agtttcagaa tatccggcac gttttgcgga
     5341 tgcagcggat cgccgccgga gagggaaatc ccctggcgtt tgatacgagt gtcattcaga
     5401 tctttaatga tcttgtcttc cattgctttg gtaaatggct gaccagaatt tacccgccat
     5461 gtgcttttgt tatagcaacc ggggcattca tgaacacacc cggagacaaa cagggtgcag
     5521 cgagtgccgg ggccgttgac gatgtcgaca ggatagtact gatgataatt cattatttcg
     5581 cctggcttat cagtccgcgt gtaggctgca tccggtaatc aatgcctgat gcgacgctgt
     5641 cgcgtcttat caggcctaca gccgttgcca aatgtaggcc ggataaggcg tttacgccgc
     5701 atccggcatt tgcttagcgc agagtgaaga ttaacctatc tgcccattcc ccaaatgttt
     5761 aacgcggcgc ttaacttctt cctgcttacc agcgttaaac ggacgtgcat ccgggctacc
     5821 taaatatccg cacacgcggc gagttaccga cacacgggag gcgtcatggt taccacattt
     5881 cgggcaagtg aagcctttgc tggtgcactc gaactcaccg gtaaagccac actcgtagca
     5941 ctcatcaatc ggtgtattgg tgccgtaata cggtacatgc tgatagctgt aatcccagac
     6001 atcttccagc gctttcaggt tgtgctgaat gtttggatac tcgccgtagc aaatgaaacc
     6061 accgttcgcc agcggcgggt aaggcgcttc aaagtcgatc ttgtcgtacg ggttcacctt
     6121 cttctccaca tcaaggtgga aactgttggt gtagtaacct ttatcggtca cgcccggcac
     6181 cacgccaaac tcagcagtat cgagacggca gaagcggtcg cacaggtttt cactcggcgt
     6241 gctgtaaaga ctgaaaccat aacccgtttc ttctttccac tgatccactg cctgacgcag
     6301 acgttcaaca atggcgatac ctttcgcgcg aagcttctcg ttgtcgtaaa catgctcgcc
     6361 gctgaacagc gcgttaatgg tttcgtggat gccgatgtaa cccagcgaaa tagacgcacg
     6421 accgtttttg aagatttcag aaacatcatc gtcagcattc agacgtacgc cacaagcacc
     6481 ttccatatag aggatcgggg cgacgcgcgc tttcacgcct tcgagacgag cgatacgggt
     6541 catcagtgcc ttacgtgcca gcaccagacg ttcatccagc agcttccaga aggtggcttc
     6601 atcgcctttt gcttccagag caatacgcgg caggttcagg ctgatcacgc cgaggttgtt
     6661 acgaccatcg tggatctgct cgccgttttc attttcccac acgccgagga agctgcggca
     6721 gcccatcggg gttttaaacg aaccggtgac gttcactacc tgatcgtagt tcaggatatc
     6781 cggatacatg cgcttgcttg cgcactccag cgccagctgt ttgatgtcgt agttcggatc
     6841 gcctttttta tggttcaggc catcgcgaat cgcaaacacc agtttcggga acaccgcagt
     6901 tttacggttt ttaccgaggc ctgcgatacg gttacgcagg atcgattcct gaatcaggcg
     6961 cgattcccag ctggtgccca gaccaaaacc aaaggttaca aacggcgtct gaccgttggc
     7021 ggtgtgcagg gtgtttactt cgtactccag cgactggaag gcatcgtagc actctttgat
     7081 ggtacgagag ttagcgtagc cttcggcgtc cgggatgttc cactcttctg cggtcttgcg
     7141 atgtttgttg tagctggcgg taacaaacgg cgccagcact tcatcgatac ggttaatggt
     7201 ggtgccgcca taaatatggc tggcgacctg agcaataatc tgcgcagtta ccgcggttgc
     7261 cgtaaagatc gacttcggcg gttcaatctc ggcgttcccc attttaaaac cctgggtcag
     7321 catgcctttc aggtcgatca acatgcagtt gaacatcggg aagaacggcg agtaatcgag
     7381 atcgtgatag tgaatatcgc cacgctcatg tgcctgcacc acgtcacgcg gcagcaggtg
     7441 ctggcgtgca tagtgtttag ccacgatccc ggccagcagg tcgcgctggg ttggaatcac
     7501 cttgctgtct ttgttggcgt tttcgttgag taacgaggcg ttggtctgct cgaccagacc
     7561 acggatctcc tggttcaggc gaccgcgttt ttcacgttca atgtcgcgat cgtgacggta
     7621 ctcgatgtaa gcacgagcca gttgtttgta tggacccgac atcagctggt tttcaactgc
     7681 ggtctggatc tcgttgatat ccacctggtt gcgcccctgc atctgctcgt tgacaaccgc
     7741 ggcaacagtg gcgcaataat cggcatcatc gacttccgct gctttagctg cacgcagaat
     7801 cgcttctttg atgcgctctg atttaaacgg cactttgcag ccgtctcgtt tcatcacatg
     7861 cggtgtcatg atcgctccat atttttaaga acaggttatc cacagaaatt gggaaaggcg
     7921 ttttccggtt ttttcgtttc gttttccggt gctttccgca atcgccatcc gcttatccac
     7981 aacgccgcac ctttttcgtg gggagctgta gtagcaatta tagtcgatta atacaacata
     8041 ttgggttggg atgcatttta aagtctatat atagtgcttt gcatcaagga tgttttaact
     8101 ttttttgatg tagctcaaag taaaaagcag agcgtacgga tgacgggcgc tacagcgtta
     8161 tgtaaatttt ttaatgaatt tgctggttga aaaatcaaca aaaacaacat gctgacagac
     8221 aaaaccccgg aatgaccggc attatcgggg cttagggaag atttacttct gtaaccacca
     8281 gacagcctca aaaggccgta aattcatggc acagggttgt ggtgaggctt cttcgtagtt
     8341 atgcatcaca agctgccagt tgccgcgcat ttgccctggc tgccagggtt ggatctcacg
     8401 gctaaggttg gcaatgacca gcaaggtttg ccccttccat tcacggcgat agcaccacaa
     8461 tacagggctg tttggcagca gatcctggta attgccccat gtcaggatgg cttcttgctt
     8521 acgcagtgcg attaactttt ggtaggtgta aaacacagag gagtcatcgg ccagcgcggc
     8581 ttctacgttg atttgttgat agttatcgcc taggccaatc cacggttcgc cagccgtaaa
     8641 cccggcatta tcgccgttgc tccattgcat gggcgtgcgg ctgttgtcac gggatttact
     8701 ggcgaggatt gccaataact cgtcggcatc acgaccatcg ttgcgcagct cggcaaacat
     8761 attgaggctc tccacgtcgc gatagtcagt aatgcgcgtg aaatgcgggt tggtcatgcc
     8821 aatctcttcg ccctggtaga tatacggcgt tccctgcatg ccatgcagca ccatcgccag
     8881 catttttgcc gcaggcacgc ggtattcacc ttcatcacca aagcgagaaa caatgcgcgg
     8941 ctgatcgtgg ttacaccaga acaaggcatt ccatgccacg ttgtgcattc cttgttgcca
     9001 gtggcggaac aatgttttca acgccacaaa gtcaggttta gccagcgtcc atttttcacc
     9061 accgggataa tcgaccttca ggtgatgaaa attaaaggtc atcgacaatt cactgcctgt
     9121 cagtgccgcg tatcgctggc aatgctcaag gctggtggag gacatttcac ctacggtcat
     9181 taacccgcgt ggcgtaaaca catcgcggtt catctcgtgc aaaaactcgt gtgctcgtgg
     9241 cccgtcggtg tagaagcgac gcccgtcgcc atccaggtct tcagggaaac gcgggtcttt
     9301 ggagatcaga ttcaccacat ccaggcgcaa cccgtcgacc ccacgatcgg cccagaattc
     9361 acagactttt ttcagctctg cgcgtaccgc tggattctcc cagttgagat ccgcctgttc
     9421 tggtgcaaag agatgcaaat agtactgttc gctttccgca tgccagcgcc acgcactacc
     9481 gccaaatttt gaacgccagt tgttcggtgg cgtttctggt tcgccatcgc gccagatata
     9541 aaactggcgg taagggcttt ctttgttcag cgcctcgcga aaccaggcat gttgggtaga
     9601 ggtatggtta aacaccatat cgagaatgat acgaatcccg cgcgattttg cctgcgtcac
     9661 cagttcgtca aaatcgtcca gcgtgccgta ggtgggatca atcgccgtat agttcgctac
     9721 atcgtaaccg ttatcgacct ggggagagac ataaaagggg gttagccaga tggcatcaac
     9781 gcccagttta tgcagatagt ccaggcgttg gataacgcca cgtaaatcgc cggtgccgct
     9841 acccgtggtg tcctgaaaac tctttggata aatctggtag ataacgccgt tttgccacca
     9901 gtggggaaga ttagtcatta cgttattcct gcaaatgcga gggggcgcaa ttgcgccccg
     9961 aagaaaatta aacaatgtcc agcgtgccca ggcggtattt ccgctgatag ataaacgagg
    10021 tgagtacaat cgggatgatg atggcgatag ccattgccag cgcaaatacc tgccagtagc
    10081 tcggttgaat agagagaatt cccggcaggc cccctacgcc gataccattc gccataacgc
    10141 cgttcaggcc gcatagcaat cctgccagac cagaaccaat catcgcgcac agcatcggga
    10201 agcgatattt caggttgatg ccgtacattg caggctcagt gaccccaagc caggcggaga
    10261 tagcggcagg cacggagatc tcgcgttcat tgtgcttgcg gctggaaatg ataatgccta
    10321 tcacggcgga gccctgagcg atattcgaca gcgcaatcag cggccacact ggcgtgccac
    10381 ccatgctttg aatcatctgc aaatcaatag caagggtggt ctggtgtacg ccggtgatca
    10441 ccagcggcgc gtacaggaag ccaaacaatg ccgcaccaat cggagcaaag ctgccggtca
    10501 tcaggtgacg taccgcaaag gcaacgccat cgccaatcat gcgaccaaac ggaccaatca
    10561 gcgcatgggc gaggaacacc gcgaggatca gcgaacagac gggcaccacc accagataga
    10621 ggtaatccgg tacgatacgt ttaaggcgag tttcaataac gcccagcgcc agcccggcta
    10681 acagtgccgg gatcacctgc gcctgatagc ccactttggc gatgctgaac atgccaaagt
    10741 cccacacttc cggcagctgc tgcccgagca gataagcgtt catcagctgg ggggaaacca
    10801 gtgtcacgcc aagcacgata ccaaggatcg gcgtgccgcc catttttttc accgctgacc
    10861 agcaaatccc gaccggcagg tagaagaaaa tcgcttcacc gatcaaccac agaaaatcgt
    10921 agatcgtttg cagggaaggg tacatttgcg ccagcgtctg accgttgctc atgggcaaat
    10981 cgccgatcac attgcgaaaa ccgaggatca aaccgccgct aatcaacgcg ggcagcaacg
    11041 ggaagaagat ctccgcgaaa tgagagatca actgctcatg ccatttcata ttttgccggg
    11101 cggctttttt tacctgctct ttatcaacct gcgcctgtcc ggttgacgct attagtgctt
    11161 gatagtaatc acccacgttg gtgccaatca ccacctgaaa ttgcccggca ttggtgaaac
    11221 agcctttcac catggggagt tgctcaattt ctttcggtct ggcattggcc ggttggttga
    11281 ggacaaagcg caggcgagta atacagtggc tcaccgtcgc aatattgccg cgcccgccga
    11341 ccagttcaat caaccgatcg atatccgttt ggtttatttt gctcatcata aagccccatg
    11401 gcagatgaca tttttggttg gctgcagaat atattgcgcg gaaaaattta aaaacgggaa
    11461 cgttcccgaa acgcagcgaa gatcacaatt tatcgttcag gaaacgatca ggacagggtg
    11521 gcggggatga tgatttgttg cggttcgctg cgcccggtta cctgtgcgat caattggcag
    11581 gccgcctggc gtccagcttc ggcgtagcca ggatctacgg tgacgatctc cggatgaagg
    11641 aatttcatta acggcgtatt acctacgctc gccagttgca aggtgtcgat gcgttgctct
    11701 tgcaggtatt tacttgcgcc aagagccagc gtgtcggttg cgcacagtaa cgcggtagtt
    11761 tcaggcgtaa tcacttttgc tacgttttca tagccttgct tcatagcaag ccctggcaga
    11821 gcggcaacgg gatgcagttt atgcgctttg cagaacgcca ggtaggcttc gtgacgtcgc
    11881 ttcccggtcg tcacgtcact atgcggcacg ccgagataac tgatattgcg atgcccctgg
    11941 tcatagagcc gttgcatcag aatcttgatt gccccttcgt cgtcataaca gaccgaagca
    12001 aagccttttg cgtcacgcgc cagcagaacc agcgatgact gccagtgggc taacatttct
    12061 tcggttatgc cggtaaaacc gaacagcact acgccgtcga tattacgccg tttcagcacc
    12121 cccaaatgtt cggcaactaa ttgtggggaa aactgatttt ccatcatgat tggatcgtaa
    12181 ccttgttcat agaacgctgg cagcatggtt tgaacggcga gattttctga caacgaatcc
    12241 agacgggtaa caatgatggc gaccacttta tcgctttgcc cacgcatagc gcgcgcagag
    12301 cgggaagggg aaaatccatg ctgattcatc actgcttcaa cacgctcgcg cgtgcgctga
    12361 ctcacgccgc tttcgttatt cagcacccgg gaaaccgtag atttccccac gccgcttaag
    12421 cgcgcgatgt ctttgatggt cagccgattt tgcatcctgt tgtcccataa cgtgttgttt
    12481 aattatttga gcctaacttt acccgtgcat tcagcaatgg gtaaagtctg gtttatcgtt
    12541 ggtttagttg ccagcaggta ttatatcgcc ataaatgcta cgaatattat tggattctcc
    12601 ttattatttg cggcgctttt ttcacttacc ggaggttata tggaacctga tcccacgcct
    12661 ctccctcgac ggagattaaa acttttccgg taagcccgtc ttttcacggc gttaccggat
    12721 gcgtaaggcc gtgacgtttt aatgtccctg ctcagcttta ttaccttcag gtaaggcttc
    12781 gccacgcctg aagacatttc tgtactgttt cagacagtgc ggagggactc cttatgttta
    12841 aagaaatttt tacccggttc attcgccatt taccttcccg tctggttcat cgcgatccat
    12901 tgcctggcgc gcagcagaca gtgaatacgg cggtcccgcc gtccttaagt gcgcattgcc
    12961 tgaaaatggc ggtgatgccc gaagaagaat tgtggaaaac gttcgacacc catccggaag
    13021 gattaaatca ggcggaagtg gaatctgccc gcgaacaaca tggtgataat aaattacccg
    13081 cccaacaacc gtcgccgtgg tgggtacatt tatgggtctg ctatcgcaac ccctttaata
    13141 ttttgctcac cattcttggt gctatttctt acgccacgga agatttattt gccgcaggtg
    13201 ttatcgcgct aatggtcgct atttctacgt tgctgaactt tattcaggaa gctcgttcca
    13261 ccaaagcggc agatgccctg aaagcgatgg tcagtaatac cgcgacggta ctgcgagtaa
    13321 ttaacgacaa aggcgaaaat ggctggctgg agatcccgat agaccagctg gtgcccggcg
    13381 atattataaa actggcggcg ggggatatga tcccggcaga tttacgtatc ttgcaggcgc
    13441 gggatctgtt cgtcgctcag gcgtcgttaa ccggtgagtc tctgcccgtg gaaaaagccg
    13501 ctactactcg ccagccggag cacagcaatc cgctggagtg cgacaccctg tgttttatgg
    13561 gcaccaccgt ggtgagcggc acggcacaag caatggtgat tgctacagga gccaacacct
    13621 ggtttggtca actggcgggg cgtgttagtg agcaggaaag cgagccgaat gcctttcagc
    13681 aagggatcag ccgcgttagt atgctgctga ttcgctttat gctggtgatg gctccggtgg
    13741 tgttgcttat caacggttac accaaaggcg actggtggga agcggcactg tttgcgcttt
    13801 cggtcgcggt aggcttaacg ccggaaatgt tgccgatgat tgttacctcg acgctggcgc
    13861 gcggcgcagt aaagctgtcg aaacagaaag tgatcgtcaa acatctggat gccattcaga
    13921 actttggcgc aatggatatt ctgtgcactg ataaaaccgg caccctgacg caggacaaaa
    13981 ttgtgctgga gaatcatacc gatatctccg gtaaaaccag cgaacgcgtg ctgcatagcg
    14041 cgtggttgaa cagtcattac cagaccggac ttaaaaatct gcttgatacc gcggtgctcg
    14101 aaggtacgga tgaagagtca gcgcgttcgc tggccagccg ttggcaaaaa attgatgaga
    14161 ttccgtttga tttcgagcgt cgtcggatgt cggtggtagt ggcagaaaat accgaacacc
    14221 atcagctggt ttgcaaaggt gcattgcagg aaatcctcaa tgtgtgttcg caggtgcgtc
    14281 acaatggcga gattgtaccg ctcgatgaca ccatgctgcg taagattaag cgggttactg
    14341 atacgctgaa tcgtcagggg ctgcgcgtgg ttgcggtggc gacgaaatac ctgccagcgc
    14401 gtgaaggaga ttaccagcgg gcggatgaat ccgacctgat cctcgaagga tatattgctt
    14461 tccttgatcc gccaaaagag acaaccgctc cggcactgaa ggcattaaaa gcgagtggaa
    14521 ttaccgtaaa aatccttact ggcgacagtg agttagttgc agcgaaagtg tgccatgaag
    14581 tggggctgga tgcgggagag gtggtcattg gtagtgatat tgaaacgcta tctgacgatg
    14641 aactggcaaa tctcgcacag cgcaccacgc tgtttgcccg cctgacgccg atgcataaag
    14701 aacgcattgt gactttactg aagcgcgaag ggcatgtggt tggctttatg ggcgatggta
    14761 ttaatgatgc gcccgcctta cgcgctgcgg atatcggcat ttctgtggac ggcgcggtag
    14821 atattgcccg tgaagcggct gatatcatcc tgctggaaaa aagcctgatg gtgctggaag
    14881 agggggttat tgagggacgt cgcactttcg ccaacatgct gaaatacatc aaattgacgg
    14941 cgagttctaa cttcggtaat gtgttcagcg tgctggtggc gagtgctttc ttgcccttcc
    15001 tgccgatgtt gccgttacac ttgcttattc agaacctgct gtacgatgtg tcacaggtgg
    15061 cgatcccgtt tgataacgtc gacgacgagc aaattcaaaa gccgcagcgt tggaatccgg
    15121 cggatctggg gcgctttatg gtcttcttcg gaccgatcag ctcgattttc gatattctga
    15181 cgttttgctt gatgtggtgg gtattccatg ccaacacgcc ggaaacgcaa acgctgttcc
    15241 agtcgggctg gtttgtggtg ggattactgt cgcaaacgct gattgtgcat atgatccgta
    15301 cccgccgtgt gccgtttatt cagagctgtg catcgtggcc gttaatgatc atgaccgtga
    15361 tcgtgatgat tgtcgggatc gcattgccgt tttcaccgct ggccagttat ctgcagctgc
    15421 aggcgctgcc gttaagctat ttcccgtggc tggttgcgat tctggcaggg tatatgacgt
    15481 taacccagtt ggtgaaaggg ttctatagcc gtcgttacgg ctggcagtaa agaataaact
    15541 gggcatggtg gcccggattc agattgcaga caaagtgagc tttgttcatg ccggatgcgg
    15601 cgtaaacgcc caatccggcc tacaaattca ataaattgca ggaatcacgt aggcctgata
    15661 agcgtagcgc atcgggcgat tttgcttttg tcatcagact taatccgggc atgatagccc
    15721 ggatttccat caagattagc gacgaacagc gattgcttcg atctcaatct tcacgtcttt
    15781 cggcagacgg gcaacttcaa cgcaagaacg tgccgggaaa gtggcgttgt gttcggtgaa
    15841 gaaggcttcg taagtggcgt ttacggttgc gaagtcgttc agatctttca cgaacacagt
    15901 ggttttaacg atgtcgccca ctttcaggcc agcggcttcg acgattgctt ttacgttatc
    15961 cagcgactga cgtgcctgtg cagcgacgtc tgccggtact tcaccagttt tcggatttac
    16021 cgggatctga ccggaggtga tgatcatatt gcccagatca acgccctgta cgtaaggacc
    16081 gatagctgcc ggtgcatttt ccgtcgcgat agttttgctc atgatttctc ctttattaca
    16141 gcggtaaaaa gtctggctca ttatagggag ccagactttt attaccaacc gcaattaatt
    16201 ggccagcacc acattatggg aaaactcttt ttcacagtat ttgcatttaa gcgcgatgtc
    16261 attggcgcgt tttcgcacgg caaagctgga tgaaaccggt tcggcatggc tgatacagtt
    16321 gctgttcggg cagaccagca cattgtcgat gcgctctggc agacttgggc gcgatttacc
    16381 caccacttca tagttgtcga tacggttaac cgtggcttgc ggtgcataca atgccagttg
    16441 gtctacttgc tcttcactca aaaaggtatt ttcgattttg atcagatctt tgcggcccat
    16501 ctcgccagaa ggcaaattca gaccaatggt gatgcgctga tctgtttcgg tcaacttgaa
    16561 cagactcaac agcttaaaac cgatctgggc ggggatatgg tcaattaccg tgccgcgttt
    16621 aatagcttca acctgcaatt tattatcgtg tgtcatctct atttcccctt acagtaccag
    16681 atcgcgattc agaaccagtg ccagtaacgc ctggcgagcg aaaatcccgt tgcctgcttg
    16741 ctggaagtac caggcgtgag gcgttttatc cacatccgtc gcaatctcat caacacgcgg
    16801 cagcggatgc agcactttca tattggcttt ggcgttgtgg agatcgctgg cgcgaagaac
    16861 aaactgcgct ttcacgttgg cgtactcgga cgggtccaga cgctcttttt gcacgcgggt
    16921 catgtacagg atgtctactt ccgccatcac ttcttcaata gagctgtgca gactccaggc
    16981 gatccctttt tcatcgagca tatccagaat gtattgcggc attgccagcg catccggcgc
    17041 gataaagtaa aaacggttgc cgtcgaactt cgctagcgcc tgggtcaggg agtgaacggt
    17101 gcggccatat ttcaggtcac caaccattgc gacgtggaga ttgtccagac gcccctgggt
    17161 ttcctgaatg gtgaataagt ccagcaaggt ttgcgtcgga tgttggttgg agccatcacc
    17221 ggcattcagt accggtacat tgccagaaaa ttcggtggct aggcgcgccg caccttcctg
    17281 cggatgacgc atcactatcg catcgacgta agtgctgata accgaaatgg tatcggccag
    17341 cgtttcgcct tttttaccca gtgatgtatt ggcgctgtcg gagaagccca ccacgctggc
    17401 acccaggcgg tgcatggaag tttcgaaaga gaggcgggta cgggtagagg cttcgaagaa
    17461 acagctggca atgactttgt gcttcaacag ctctggttgc gggtttgctt tcagtttcgc
    17521 cgctgtcgcc agcaccagat taaggtcatc gcgactaagg tcgtttatgg aaatgatatg
    17581 tttctgatat agcggattag ccatctttta tctcctgaca cctaggcaaa aaaaagcccc
    17641 tcgattgagg ggctgggaat gggtgatcaa cgggaagaaa aacggcaggc cagcgtcttt
    17701 tttcagacgc ggtaagacaa aatgtcgaac acactgaacc atacatcctc ccggcaaatt
    17761 gtccggcatt atactcatag ccagaagcgg tgcaagcatt tgatgcaata ttttgtcagc
    17821 acaaacggtt tatttgaatt aaaagtcaaa atatatgcat ttttattttc tatgattctg
    17881 gaggggaagt gtcgttcggt atcaataatt gcagacattt cctgcttttt tctttttcat
    17941 tttcacgcaa tcagaaatgc attttattcc attcatccga cttatagggc ggagggtgaa
    18001 aacgaacggc taacactatt gcttatagct cagggatacg cgctatcact ttaatttcaa
    18061 aatcaaagcc tgccagccag gtaacaccca ccgccgtcca gtttggataa ggtggggcgc
    18121 taaatatttc atttttcacc gtcatgatgt cttcaaattg tttttccgga tcggtatgaa
    18181 agctcgtaac atcaatgata tcgtcaaaag tgcatcccgc agctgccagg gtcgcatgca
    18241 aattatcaaa ggccagtctg acttgttgct gaaaatcggg ttctggtgtt ccgtcctctc
    18301 gacttcctac ttgcccggaa acaaacagca aatcgccgga acgaatagcc gcagaataac
    18361 gatgctcagc atacagtgaa tgtcggccag cagggaaaac agcggttctt tctaccattt
    18421 ggttatcctc aagatttacg acatgaacag aagatttata tttatcggga gccgctttta
    18481 gggggacgac gttagtaaac aaaacccaga catcatggat aatggctggg cttaattgag
    18541 cgtagtcggt tatgcgccaa acgcgccatc aatggtatgc atcgcgccgg taacaaaact
    18601 ggcttctggc cctgctaacc atgcgaccat accagcgacc tcttccggtt gcccatgtct
    18661 tttgatggcc ataaaactat gcaacatatc gcgcattggc ccgttggcgg gattaacgtc
    18721 ggtatcaatt ggtcctggct ggacgacgtt gatggtgatc cctcgcggtc caaaatcacg
    18781 ggccagcccg cgcgccatgc cttgcagggc agatttactg gcggcataag cagccatgcc
    18841 tgcaacaggc atacgatcgc cattcacgga gccgatgatt aagatgcgcc cgccttcggg
    18901 catctgccgg gcggcttcaa cagaggcatg ataaggagca tgaatattga ttttgaaaag
    18961 gcgatcaata tcgtcggcat ttaattccag ggcatcgcca aagacgccaa tacctgcatt
    19021 taccaccagg atatccaatg cgccgctctt acgaacgaca tcaataacag cgtctctgtc
    19081 agcactatct gtgaatactg ctgtcgctcc agtctcttgt gccagacgtt cagcggcatc
    19141 tttcgacccc gcataggtga atcgtacatt ggccccatcg gtgacgaaac gacgtacgat
    19201 agcggcaccg attccacgac tgccaccgag gatgagaact gtcttaccgg taaaagcgcc
    19261 cataaggact ccttgattta ttatgtaaca tgcattacaa aactgtttta actttctgtc
    19321 aacatgtttt gtaatggtca ctaaaaaaca atctcgcgtt ccaggtcgtc ccagacgttt
    19381 cgctcctgag caggcagtct ctgcggcaaa agtgcttttt caccaaaaag gtttcgatgc
    19441 agtaagtgtc tctgaagtta ctgattatct tggtattaac cccccgagtc tctacgcggc
    19501 ttttggcagt aaggctgggt tatttagccg tgtactcaat gaatatgtcg gtacggaagc
    19561 tattccgctt gccgatattc ttcgtgatga tcgtccggta ggcgagtgcc tggttgaggt
    19621 attaaaagaa gcggcgcgca gatatagcca aaacggcggc tgcgctggct gtatggttct
    19681 tgaaggtatt catagtcatg atccacaagc gcgtgatatt gccattcaat attatcacgc
    19741 cgcagaaacg accatttatg actatatcgc caggcggcat ccacaaagcg cacaatgtgt
    19801 gactgatttt atgagtaccg tgatgtcagg gctttctgcg aaggcacggg aggggcactc
    19861 catcgaacaa ctctgtgcaa cagctgccct ggcaggggaa gcgataaaaa ctcttctcaa
    19921 ggagtgatgc tggccttgat ccgaaaggcg ggaacgcgcc tgccgataag ttgtgataag
    19981 acaataattc acgcgttaag gctggcggaa ttgatcatct tttcgtataa cgatagaaat
    20041 gaaacattat tttaattaag gagtggaaaa gatgatcatc ggaaatattc ataatcttca
    20101 gccgtggcta ccccaggagt tacgccaggc gattgagcat atcaaagcac acgttacggc
    20161 agaaacgcca aagggcaagc atgatatcga aggcaatcgt ctgttttatc ttatctcgga
    20221 agatatgacc gagccgtacg aagctcgccg tgcggagtac catgcccgct atcttgacat
    20281 tcagattgtg ttaaaaggtc aggaaggcat gaccttcagc acgcaacctg caggcgcgcc
    20341 ggataccgac tggttagctg ataaagacat cgcatttttg ccggaaggcg ttgatgagaa
    20401 aacagttatc ctaaatgaag gtgattttgt tgtgttttat ccgggggaag tgcataaacc
    20461 gctgtgcgca gtgggcgcgc cagcccaggt tcgcaaagca gtagtgaaga tgctgatggc
    20521 gtgatgactt ttcgccgtaa ataactccag gtttacggcg agtttgtgaa aagagcgttt
    20581 tttgatattt ttttgtgagt aaaatttgta atgcttagac gttcttatta actcaaggag
    20641 ttcgtcatgg gcaaaatatc agatttgaat tattctcaac acattacatt agccgacaat
    20701 tttaaacaaa aaaatgaagc cttagatacc tggtatgtgg gaatgaatga ttttgcccga
    20761 attgccgggg ggcagaatag cagaagcaat attctttctc ctagagcatt tttggagttt
    20821 ttggcaaaga tatttaccct gggttatgtg gattttagca aacgctccaa cgaagcgggt
    20881 agaaatatga tggctcatat tgagttctca tcttattcta aagatactga tggcaatgaa
    20941 aaaatgaagt tttacatgaa taatcctgaa ggggagcgag cggatttatc taaggtgaag
    21001 atagaaatta cacttgc
//
LOCUS       DAJVPP010000070        20942 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVPP010000070 DAJVPP010000000
VERSION     DAJVPP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.070"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..696
                     /locus_tag="OQM34_002632"
     CDS             <1..696
                     /locus_tag="OQM34_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCS1453019.1"
                     /translation="ENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKR
                     YRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVIS
                     YSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMS
                     RKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLT
                     PIEYRNQTYMPRV"
     gene            798..950
                     /gene="hokA"
                     /locus_tag="OQM34_002633"
     CDS             798..950
                     /gene="hokA"
                     /locus_tag="OQM34_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1453020.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1137..1349)
                     /gene="cspA"
                     /locus_tag="OQM34_002634"
     CDS             complement(1137..1349)
                     /gene="cspA"
                     /locus_tag="OQM34_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1453021.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1630..1920)
                     /locus_tag="OQM34_002635"
                     /pseudo
     CDS             complement(1630..1920)
                     /locus_tag="OQM34_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2354..3064
                     /locus_tag="OQM34_002636"
     CDS             2354..3064
                     /locus_tag="OQM34_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1453022.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3114..4088)
                     /gene="ghrB"
                     /locus_tag="OQM34_002637"
     CDS             complement(3114..4088)
                     /gene="ghrB"
                     /locus_tag="OQM34_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1453023.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4192..4842)
                     /locus_tag="OQM34_002638"
     CDS             complement(4192..4842)
                     /locus_tag="OQM34_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1453024.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            5005..7338
                     /gene="bisC"
                     /locus_tag="OQM34_002639"
     CDS             5005..7338
                     /gene="bisC"
                     /locus_tag="OQM34_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS1453025.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7307..7747)
                     /locus_tag="OQM34_002640"
     CDS             complement(7307..7747)
                     /locus_tag="OQM34_002640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1453026.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7744..8307)
                     /gene="tag"
                     /locus_tag="OQM34_002641"
     CDS             complement(7744..8307)
                     /gene="tag"
                     /locus_tag="OQM34_002641"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1453027.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8465..9163
                     /locus_tag="OQM34_002642"
     CDS             8465..9163
                     /locus_tag="OQM34_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1453028.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9392..10600
                     /locus_tag="OQM34_002643"
     CDS             9392..10600
                     /locus_tag="OQM34_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1453029.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10924..12615
                     /gene="eptB"
                     /locus_tag="OQM34_002644"
     CDS             10924..12615
                     /gene="eptB"
                     /locus_tag="OQM34_002644"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1453030.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12707..12783
                     /locus_tag="OQM34_002645"
     tRNA            12707..12783
                     /locus_tag="OQM34_002645"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12741..12743,aa:Pro,seq:cgg)
     gene            13527..15134
                     /gene="dppA"
                     /locus_tag="OQM34_002646"
     CDS             13527..15134
                     /gene="dppA"
                     /locus_tag="OQM34_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCS1453031.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTLHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15442..16461
                     /gene="dppB"
                     /locus_tag="OQM34_002647"
     CDS             15442..16461
                     /gene="dppB"
                     /locus_tag="OQM34_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1453032.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16471..17373
                     /gene="dppC"
                     /locus_tag="OQM34_002648"
     CDS             16471..17373
                     /gene="dppC"
                     /locus_tag="OQM34_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1453033.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17384..18367
                     /gene="dppD"
                     /locus_tag="OQM34_002649"
     CDS             17384..18367
                     /gene="dppD"
                     /locus_tag="OQM34_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1453034.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18364..19368
                     /gene="dppF"
                     /locus_tag="OQM34_002650"
     CDS             18364..19368
                     /gene="dppF"
                     /locus_tag="OQM34_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1453035.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19398..20669)
                     /locus_tag="OQM34_002651"
     CDS             complement(19398..20669)
                     /locus_tag="OQM34_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1453036.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5069 a         5146 c         5475 g         5252 t
ORIGIN      
        1 gagaatagag gccgatacgg ataccgtagg gtaacgctgt ctcttcatcg agaagggaaa
       61 cagattaacc ataaagctgt tcagcgcctg atgggaaccc tctcacttaa agcagcgatt
      121 aaggtcaagc gataccgctc ttacagagga gaggtagggc aaaccgcccc taatgttctc
      181 caaagagatt tcaaggctac gcggccaaac gagaagtggg ttaccgatgt tactgaattt
      241 gcagtcaatg ggcgcaagct gtatttgtct ccagtaatag atctcttcaa caacgaagtt
      301 atttcttaca gcctttcgga aagaccagtg atgaacatgg ttgagaatat gctcgatcag
      361 gcattcaaaa agcttaatcc tcacgagcat cctgttctgc actctgacca gggatggcag
      421 tatcgtatga gaagatatca aaatatcctt aaagaacatg gtattaaaca aagcatgtcc
      481 agaaaaggca attgtctgga taatgctgtg gtggagtgtt tctttggaac cttaaagtcg
      541 gagtgttttt atcttgatga gttcagtaat ataagcgaac tgaaggatgc tgttacggaa
      601 tatattgaat actataacag cagaagaatt agcctgaaat taaaaggtct gactccaatt
      661 gaatatcgga atcagaccta tatgcctcgt gtttaactgt ccaacttttt ggggtcagta
      721 caaagtgagg ctaccctatg cctgaacacc ataaggttag cctcttactc gttggaaatc
      781 aacacagggg gctgggaatg ccgcagaaat atagattact ttctttaata gtgatttgtt
      841 tcacgctttt atttttcacc tggatgataa gagattcact gtgtgaattg catattaaac
      901 aggagagtta tgagctggcg gcgtttttag cctacaaatt gaaagagtaa gagtcttcgg
      961 cgggaaatta ttcccgcctt acttacggcg ttgcgcattc tcattgcacc catatttatt
     1021 cttcacaaaa ataataatag attttattac gcgatcgatt atttatttcc tgaaaaaaaa
     1081 taaaaaaatc cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac
     1141 aggctggtta cgttaccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact
     1201 ttctgacctt cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac
     1261 acatctttag agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat
     1321 tttacgatac cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct
     1381 ttcggcgata tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg
     1441 gtatcaacga taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt
     1501 caaaccgttg atgtgcatta agccactcat tggcgggtga tgcaacaatt atttttcata
     1561 tttatgatta atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg
     1621 gataaaagtc tatttcatca actgcttact taatgccggg ttggcttgaa tcaaacgcat
     1681 caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc
     1741 gactgatacg cccaaaaccc gggcgaaatc atcgattttt aatcctgtcc cttttcataa
     1801 ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc
     1861 tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat
     1921 tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag
     1981 tcgggaaaag cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca
     2041 gcctgtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct
     2101 tatcactcct gttttgtaaa gatttcgcaa atctaacctt ctgcgaaggc gtaaatctct
     2161 ggctacaaaa aaggcaacgg cactatcatt ttttaacaat gaaacaacaa attaaccttg
     2221 tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg
     2281 tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga
     2341 aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc
     2401 gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc
     2461 gtttatcgac ttcctgcaaa atacagtgat gcgtagcggt gaacgtctac caaccctgac
     2521 tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta
     2581 ttctcagcag gtgaatcagg cgatggattc cggtctgcgc ccggtcgtag acagcgttaa
     2641 cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg
     2701 ttcgctgggt gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca
     2761 ctctgcgcta aaacagagcg atgacctgaa accggtattt gatcaggcat tcaccaaagt
     2821 ggtaacgacg ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca
     2881 acaactggtg atggttggag actacattgc tcagcaaggt actcaggtga gctttgtcgc
     2941 aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc
     3001 attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca
     3061 ataaagagta aaagcccgag cgattatcgg gctttacttc acgcagtcgc agcttagtcc
     3121 gcgacgtgag gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta
     3181 tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt
     3241 actgcgacga cgttggccat tgagagcaac ggcgaatcta cggacagtgg ctcttgttcg
     3301 aagacatcca gcccggcggc gtgaatttcg cctttctgca atgctgcgat cagtgcattt
     3361 tcgtcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg
     3421 aattgttctg cgccaaacag atgatgcgtc tcatcagtta acggcaggat caggcaaacg
     3481 aaatctgact cttgtaacag agtatccaaa tcgcagtagc gggcgttgaa gcgttcttct
     3541 gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcaca
     3601 cgttgtgcca gcgccatgcc gatccgtccc atcccgacaa tgcccagtgt tttatggtga
     3661 acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt
     3721 tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg
     3781 acggtttctg ttaatacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca
     3841 tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt
     3901 ttttccagca atgcggcatc tacattctcg tttgaaccca gtaaaccttc agcttctgca
     3961 aaaattgctg cattttgaac gacggtttgt gggctgaggt ttgccacctg gtgaacggtg
     4021 aaatgcgctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac
     4081 ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa
     4141 ggaaaactga agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg
     4201 gcttaaggta atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc
     4261 gattgggtta gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt
     4321 gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcaggtcgtg
     4381 accccccgtg ctgtcggtat aaccaatcac gttaaccgcc gttttcggat actctttcag
     4441 caccattgcc acgccggtca gggtgttagc gcctgccggt ttcaggggag cgctgctgct
     4501 gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct
     4561 aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc
     4621 cacgccgccg cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc
     4681 tttcttcgaa gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc
     4741 gatagcagat ttgcctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac
     4801 cgccagagcg ccactcacta cggcggcaag taagataaac acgtttcttc attgttaatc
     4861 cttaataacc tttttattct ttgtcacggg ttccgtggcg ggagattatg ccgcgtgaac
     4921 atgaagatta ttcctgggaa tactcggaaa tttgtaagta atatttaact gctcaataca
     4981 tctaaccttt caggagtctt cggtttggcc aactcatcct cacgatattc cgttctgact
     5041 gccgcccact gggggcccat gctggttgaa accgacggcg aaaccgtgtt tagctcgcgt
     5101 ggcgcgttag ccacaggaat ggaaaactcc ttgcagagcg cggttcgcga ccaggttcac
     5161 agcaatacgc gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc accggaaaac
     5221 ccgcaaggca ttcgtgggca ggatgaattt gttcgcgtga gttgggatga ggcgctggat
     5281 cttattcacc aacaacataa acgcattcgt gaggcttatg gtccggcatc gatttttgct
     5341 ggttcctacg gctggcgttc aaacggcgtg ctgcataagg cctcgacatt attacaacgc
     5401 tatatggcgc tggcaggcgg ttataccggg catctggggg attattcgac cggtgcggca
     5461 caggcgatca tgccgtatgt cgtaggtggc agcgaagttt atcaacagca aaccagttgg
     5521 ccgctggtgc tggaacatag cgatgtcgtg gtgttgtgga gtgctaaccc actcaatacg
     5581 ctgaaaattg cgtggaatgc atccgatgag caggggcttt cttacttttc cgcactgcgt
     5641 gacagtggga aaaagctgat ctgcattgat ccaatgcgat cggaaaccgt cgatttcttt
     5701 ggcgataaaa tggagtgggt ggcaccgcac atgggcaccg atgttgcgct gatgctgggg
     5761 atcgcccata cgctggtgga aaatggttgg cacgacgaag cgtttctggc gcgttgcacc
     5821 acaggttatg ccgtcttcgc ctcttatttg ctgggcgaga gtgacggaat agcgaaaaac
     5881 gccgaatggg cggcagagat ttgtggtgtt ggcgcagcga aaatccgcga gctggcggct
     5941 attttccacc aaaataccac catgctgatg gcaggctggg gaatgcagcg ccaacagttt
     6001 ggtgagcaaa aacactggat gatcgtcacg ctggcagcaa tgttggggca aatcggcaca
     6061 cccggcggcg gttttggtct ttcttaccat tttgccaatg gtggtaaccc cacgcggcgt
     6121 tctgcggtgc tctcttccat gcagggcagc ttgccgggtg gctgcgatgc ggtggataaa
     6181 atccctgttg cccgcattgt tgaagcactg gaaaaccctg gtggcgcata tcaacacaac
     6241 ggtatgaacc gacatttccc ggatattcgt tttatctggt gggcgggcgg tgccaacttt
     6301 actcatcatc aggataccaa tcgtctgatc cgtgcctggc aaaaaccgga gctggtggtg
     6361 atctctgaat gcttctggac ggcggcggcg aaacacgcgg atattgttct gcctgcgact
     6421 acctcttttg agcgtaatga tctcaccatg accggtgatt acagtaatca gcatctggtg
     6481 ccgatgaagc aagtggtgcc gccacgctat gaagcgcgta atgattttga tgtttttgcc
     6541 gagttgagtg aacgttggga gaagggcggc tatgcgcggt ttacggaagg aaaaagtcag
     6601 ctgcaatggc tggaaacgtt ttataacgtt gcccggcagc gcggggcaag ccagcaggtt
     6661 gaattgccgc catttgctga gttctggcaa gccaaccagt taattgagat gccggaaaac
     6721 ccggacagcg agcggtttat tcgcttcgct gatttttgcc gcgatccgct ggcgcatccg
     6781 ttaaaaaccg ccagcggcaa gattgaaatc ttctcacagc gtattgccga ttacggttac
     6841 ccggattgcc ctgggcatcc aatgtggctg gagccggacg aatggcaggg caatgccgaa
     6901 ccagaacagt tgcaggtact ttctgcccat ccggcgcacc gcctgcacag ccagctgaat
     6961 tacagttctc tgcgcgaatt gtacgcggta gcaaaccgtg agcctgtcac cattcatcct
     7021 gacgatgctc aggcgcgcgg cataaccgaa ggggatatgg tgcgggtgtg gaactcccgt
     7081 gggcagatcc ttgccggagc ggtcattagc gagggaatta aacctggcgt gatttgcatt
     7141 cacgaagggg catggccgga tctggattta accgctgacg gtatttgtaa aaacggcgca
     7201 gtgaacgtgc tgaccaaaga tctccccagc tcgcggctgg ggaatggctg tgcgggtaat
     7261 acgacgctgg catggctgga aaaatacaac ggtccggaac tgacacttac agcgtttgaa
     7321 ccaccggcca gctcataatc caggtgggta gttgggtttc atcctgccat gcgcaatcga
     7381 caatgtgaaa accctgcgca cggtaaaaat ctatcgccgg ttgatttttt tgataaacct
     7441 ccagcatcag atggggatag cgctgctgca catactgcat cagcgcctta ccaataccgc
     7501 gtctgacggc cttcggtgcg acaaacattg ctgccagaaa tcggccttcc ataatgctga
     7561 caaaaccgag aagcttaccg tcctcttccc agacccagtt ttgcgcgttg gcaagatagg
     7621 catcccgcac cagcggaatg cagtcacgcc agtaattcgc ttttataaag ggatgccccc
     7681 aggttgtact ttccagccac agttcgagga tcgcggggag ttctgaacgt tgcgcttccc
     7741 gaatcatggt ttatttccca gatagcaaca gcagccaacc acatgatcat tcaccagccc
     7801 acatgcctgc ataaaggagt aacagattgt ggtgccgaca aacttaaaac cacgtttttt
     7861 cagtgcctta gatagggcgt cggaggcgga cgtagatgtg ggaatttcgc tcaacgttgt
     7921 ggcttgtgtc acctgtggct gatgatttac aaacgaccag acaaagtcgg gaaacggttc
     7981 gccgttctgt tccatttgca ggtacgcccg cgcattacca ataattgcct gaattttccc
     8041 tcgatggcgg ataatcccgg cgtcctgtac cagtctttcg acatcctctt cctgcattgc
     8101 tgcgaccttc accggatcga actgatgaaa gcaggcgcga tagttttcgc gttttttgag
     8161 gacggtgatc cacgataacc cagcctgctg cccttcaaag cagatcattt cgaacagttt
     8221 tttactgtca gtttcaggca cgccccactc attatcatgg taggcaatat aaagcgggcc
     8281 ctgactcacc cagccgcaac gttccatact ttccctcgct atgtacccga tcgtaaaaat
     8341 ttcagccgct tttcttgacg catcggtgaa aaagtcaata tttaatacag ggctactatg
     8401 cccgatatcc ttcatacaat gacaataata ttgccgaatt cggctcttcc ctggagtcgt
     8461 gttaatgatc ataaaaaaaa gcggtggtcg ctggcagcta agcctgctgg cgagcgtggt
     8521 aatcagtgcc ttttttctca acacagctta cgcctggcaa caagaatata tcgttgatac
     8581 gcaacccgga cattccacag agcgttacac ctgggatagt gatcatcaac ctgattacaa
     8641 cgatattttg tcgcaacgta ttcaaagtag ccaaagggcg ctgggactgg aagtcaatct
     8701 ggcggaagaa actcctgtgg atgtgaccag cagtatgagt atgggctgga attttccttt
     8761 atatgaacag gttacaaccg gcccggtcgc ggcattacat tacgatggca caaccacctc
     8821 gatgtataac gagtttggcg acagtactac cacgctgacc gatccgttat ggcatgccag
     8881 cgtgagtagc ttaggctggc gtgttgactc ccggcttggc gatctccgac cctgggcgca
     8941 aatcagctat aaccagcaat ttggtgagaa tatctggaag gcgcaatcag gcctgagccg
     9001 gatgacggcg acaaaccaga acggcaactg gctggatgtc accgtaggcg ctgatatgtt
     9061 gctcaatcaa aatattgccg cctatgctgc gctaactcag gcagaaaata ccactaataa
     9121 tagcgactat ctgtatacga tgggggttag cgccagattt taacgtaaca gtcacaattg
     9181 aaaccattga ataacaacag ttgtggcgat agtgggtgct aacttaccaa ataataaatt
     9241 tggtgaataa ttgtcgcgtc attcattcct gaactaaggc atttcattcc gttctgatgg
     9301 catttcatgc cgtttttccc caagcataaa atgcacttcg ttatggttgt cggcagagat
     9361 ttttcctttt tattactgca ggaatactgc catgacacct tcaaattatc agcgtacccg
     9421 ctggctgaca ctcatcggta ctatcattac ccagtttgcg ctggggtcgg tttatacctg
     9481 gagcctgttt aatggcgcgc tttctgccaa gctggatgcg ccggtaagcc aggtcgcttt
     9541 ctctttcggc ttgttaagtc tggggctggc aatttcgtct tctgttgcgg gcaaattgca
     9601 ggaacgtttt ggcgttaaac gcgtcaccgt ggcttccggc attttgctgg gattaggctt
     9661 cttcctgacc gcgcattcca acaacctgat gatgctgtgg ttaagcgccg gtgtgctggt
     9721 cggtctggcg gatggcgcgg gttacctgct gacgctctct aactgcgtga agtggttccc
     9781 ggagcgtaaa ggtctgatct ccgcgttcgc tatcggttct tatggtctgg gtagcctggg
     9841 tttcaaattt atcgacacgc agctgctcga aacggtgggt ctggaaaaaa cctttgtgat
     9901 ttggggagcg attgcgctgg tgatgattgt tttcggcgca acgttaatga aagacgcacc
     9961 aaaacaggaa gtgaaaacca gcaatggtgt ggtggagaaa gattacacgc tggcagagtc
    10021 gatgcgtaaa ccgcagtact ggatgttagc ggtaatgttc ctgactgcct gcatgagcgg
    10081 cctgtacgtg attggggtag cgaaagatat cgcccaaagt ctggcacacc ttgatgtggt
    10141 ttccgcagcc aatgcagtaa cggttatttc catcgctaac ctttcaggtc gtctggtgct
    10201 gggcattctg tctgacaaaa ttgcccgtat ccgtgttatc accattggtc aggtgatttc
    10261 gctggtgggt atggcggccc tgctgtttgc accattaaat gcagtgacgt tctttgcagc
    10321 gattgcctgc gtggcattta actttggcgg cactattacc gtctttccat cactggtcag
    10381 tgagttcttc ggcctcaata acctggcgaa aaactacggt gtgatttacc tcggtttcgg
    10441 tatcggtagc atttttggtt cgattatcgc ctcactgttt ggcggtttct atgtgacctt
    10501 ctacgtgatt ttcgccctgc tgattctgtc attggcgctt tctacgacga ttcgtcagcc
    10561 agagcagaaa atgttgcgtg aggcgcatgg ctccctttaa tgctcagccg cgagctactg
    10621 ggcttcagct tccgtgtgca tcaaacgcat caacgaaatg tataaacaat gactcgtaaa
    10681 atttgggcca gttaactggc cctttttact gctttttgta acgctaaagt ctcttttcaa
    10741 acttgcattt ttgtaaattt gtgcttcatg cacactcttt ccccacactt tttcgctttg
    10801 ctgtggtcta cttattcgcg cgtgtagatt ttacttatct gactacctcc gcacttttcc
    10861 cctgccgggc ctgaaaagcc gctaagcagg gtgttatcac ctgtttgtcc agggtttgtt
    10921 tgcatgagat acatcaaatc gattacacag cagaagctga gctttttgct tgcaatctat
    10981 attggccttt ttatgaatgg cgcggttttt tatcgccgct tcggcagcta tgcgcacgat
    11041 tttaccgtct ggaaaggcgt ttctgctgtt gttgaactgg ccgccaccgt actggtgacc
    11101 ttctttttac tacgtcttct atcgctgttt ggccgccgca gctggcgtat tctggcgtcg
    11161 ctggtggtgc tcttttccgc aggtgccagc tattacatga ccttccttaa tgtggtcatt
    11221 ggttatggca tcatcgcttc cgtcatgacc accgatatcg acctgtcaaa agaagttgtt
    11281 ggtctgaact ttattctctg gttaatcgcc gttagtgcat tgcctcttat ccttatctgg
    11341 aataaccgct gtcgctacac cttgctccga caactgcgaa ccccagggca gcgtattcgc
    11401 agcctggtgg tcgtcgtact ggcgggtatt atggtttggg caccgattcg tttgctggat
    11461 atccagcaga agaaagtgga gagggcgacc ggcgttgatt tgccgagtta tggcggtgtc
    11521 gtagcgaact cttatctgcc atcaaactgg ctttctgcgt tggggctgta tgcctgggcg
    11581 cgggtcgatg aatcttccga taataattca ttgcttaatc cggcgaagaa gtttacatac
    11641 caggctccac aaaacgttga tgatacttat gtcgtgttta tcatcggtga aaccacgcgt
    11701 tgggaccata tgggtatttt cggctatgag cgtaatacca cgccgaaact ggcccaggag
    11761 aaaaatctgg cggcgttccg tggttactcc tgtgataccg caaccaaact ctcactgcgt
    11821 tgcatgtttg tacgtcaggg gggcgcggaa gataatccgc agcgcacatt aaaagaacag
    11881 aacattttcg cggttctgaa gcagttagga ttcagttctg acctctacgc tatgcagagc
    11941 gaaatgtggt tctacagcaa cacgatggca gacaacattg cttatcgtga gcagattggt
    12001 gcggagccac gtaatcgtgg caagccggta gatgatatgt tgctggtaga cgaaatgcag
    12061 caatcgctag ggcgcaaccc ggatggtaag catctgatca ttctgcatac caaaggttcg
    12121 cattttaact acacccagcg ttatccgcgt agcttcgcgc agtggaagcc ggaatgtatt
    12181 ggtgttgata gcggctgtac caaagcgcag atgatcaact cctatgacaa ctcggtgacc
    12241 tatgtggatc actttatctc cagcgtgatt gatcaggttc gcgataagaa agcgattgtg
    12301 ttctacgcag ctgaccacgg tgagtcaatt aatgaacgcg agcacctgca cggcacgccg
    12361 cgtgaactgg caccgccgga gcagttccgc gtaccgatga tggtctggat gtcagataaa
    12421 tatctggaaa atccggccaa tgcgcaggcg tttgcgcagc tgaaaaaaga agccgacatg
    12481 aaagtgccac gccgtcacgt agagctgtac gacaccatca tgggttgtct tggctatact
    12541 tcaccggatg gtggaattaa cgaaaacaac aactggtgtc acatcccgca gacaaaagtg
    12601 gcagcggcta actaagcggc ctgctgactt tctcgccgat caaaaggcat tttgctatta
    12661 agggattgac gagggcgtat ctgcgcagta agatgcgccc cgcattcggt gattggcgca
    12721 gcctggtagc gcacttcgtt cgggacgaag gggtcggagg ttcgaatcct ctatcaccga
    12781 ccaaattcga aaagcctgct caacgagcag gtttttttgc atttgtagct catgaggatg
    12841 agaacctccg ggggcaggga ggctcgactc gacgtaggcc tgataagacg cgccagcgtc
    12901 gcatcaggca acggcaccgc acttattgtc ggatgcggcg cgaccgcctt attcgaccaa
    12961 cacaccacac attttcctgc caactttgcg ccccatatct cactatccat taacggattt
    13021 gtgacagaaa ccattgtctt tttttatatt tgcttaaatc ttttttgcgt tacttatggc
    13081 tggcgctggc attttccgct gtgcgtttta gcgttcatgg tattaatcgc tcagatatcc
    13141 accttttcct cagatttttt tcattaaatg cataaaactt aatcaccaaa tgttgctaaa
    13201 tattaagctg attgttctgg ttatgttgga gtagcacaaa tttctctgct gcaaatagct
    13261 gtttgaagtt tttttaatct ttgtttgtgg attctcaccg ttggtgtaaa tcccggttgg
    13321 ctgtattgac gttttcacat tctgttgaca gattgtaggt cacgaggggc attttatgga
    13381 ggatccgcac tgttacactg atgttaatta gtacggcatc cccacctcat aacgttgacc
    13441 cgaccgggca aaaaacaaaa aaggtcaggc agcgacaacc cactgcaaag ggttaaaaca
    13501 acaaacatca caattggagc agaataatgc gtatttcctt gaaaaagtca gggatgctga
    13561 agcttggtct cagcctggtg gctatgaccg tcgcagcaag tgttcaggct aaaactctgg
    13621 tttattgctc agaaggatct ccggaagggt ttaacccgca gctgtttacc tccggcacca
    13681 cctatgacgc ctcttccgtc ccgctttata accgcctggt tgaatttaaa atcggcacca
    13741 ccgaagtgat cccgggcctc gctgaaaagt gggaagtcag cgaagacggt aaaacctata
    13801 ccctccatct gcgtaaaggt gtgaagtggc acgacaataa agaattcaaa ccgacgcgcg
    13861 aaatgaacgc cgatgacgtg gtgttctcgt tcgatcgtca gaaaaacgcg caaaacccgt
    13921 accataaagt ttctggcggc agctacgaat acttcgaagg tatgggcttg ccggagctga
    13981 tcagcgaagt gaaaaaggtg gacgataaca ccgttcagtt tgtgctgact cgaccggaag
    14041 cgccgttcct cgctgacctg gcaatggact tcgcctctat tctgtcaaaa gaatatgctg
    14101 acgcgatgat gaaagccggt acaccggaaa aactggacct caacccaatc ggaaccggtc
    14161 cgttccagtt acagcagtac caaaaagatt cccgtatccg ctacaaagcg tttgatggct
    14221 actggggcac caaaccgcag atcgatacgc tggtcttctc tattacccct gatgcttccg
    14281 tgcgttacgc gaaattgcag aagaatgaat gccaggtgat gccgtacccg aacccggcag
    14341 acatcgctcg catgaagcag gataaatcca tcaacctgat ggaaatgccg gggctgaacg
    14401 tcggctacct ctcgtataac gtgcagaaaa aaccgctgga tgacgtgaaa gttcgccaag
    14461 cactaaccta cgcggtgaac aaagacgcga tcatcaaagc ggtttatcag ggcgcgggtg
    14521 tatcagcgaa aaacctgatc ccgccaacca tgtggggcta taacgacgac gttcaggatt
    14581 acacctacga tcctgaaaaa gcgaaagcct tgctgaaaga agcgggtctg gaaaaaggtt
    14641 tctccatcga cctgtgggcg atgccggtac aacgtccgta taacccgaac gctcgccgca
    14701 tggcggagat gattcaggca gactgggcga aagtgggcgt gcaggccaaa atcgtcacct
    14761 acgaatgggg tgagtacctc aagcgtgcga aagatggcga acatcagacg gtaatgatgg
    14821 gctggactgg cgataacggg gacccggata acttcttcgc caccctgttc agctgcgccg
    14881 cctctgaaca aggttctaac tactcaaaat ggtgctacaa accgtttgaa gatctgattc
    14941 aaccggcgcg tgctaccgac gaccataaca aacgcgttga actgtacaag caagcgcagg
    15001 tggtgatgca cgatcaggct ccggcactga tcatcgctca ctccaccgtg tttgaaccgg
    15061 tacgcaaaga agtcaaaggc tatgtggttg atccattagg caaacatcac ttcgaaaacg
    15121 tctctatcga ataattaaaa gccatacaag actgatggca aaggcaaaac tgcctgatgt
    15181 gctccgctta tcaggcctac gaaaattctg caatgtattg aatttgcacg attttgtagg
    15241 ccggataagg cgttaacgcc gcatccggca taaacaaagc gcactttgtc aacaatctgt
    15301 atacccggtg gcgctgtgcc tctgcgtgca gcgccatccg gcagcaatac ttcattctct
    15361 gtccgacaag ggcgggaatt gatttgtgag caatacagac acgcagttcc aggctgcgag
    15421 tcactataga gaatccgggt tatgttgcag tttattctcc gacgtttggg actcgtcatc
    15481 cctacgttta tcggtattac ccttctcaca tttgcctttg tccacatgat cccgggcgat
    15541 ccggtgatga tcatggcagg cgaacgtggg atctcccctg agcgtcacgt gcagctgctg
    15601 gctgaactcg gcttagataa accgatgtgg cagcagtatc tccattacat ttggggcgta
    15661 atgcacggcg atctaggcat ttcaatgaaa agccgaattc cagtatggga agagttcgtg
    15721 ccgcgctttc aggccacgct ggaacttggc gtctgcgcga tgatttttgc tacggcagtc
    15781 ggtattccgg ttggtgtgct ggctgcggtt aaacgcggtt ccattttcga tcacactgca
    15841 gttggcctgg cgctgaccgg ctactcgatg ccgatcttct ggtggggcat gatgctgatc
    15901 atgctggttt cggtgcactg gaacctgacg cccgtctccg gtcgcgtgag cgatatggtg
    15961 ttcctcgatg actccaatcc gttaaccggt tttatgctaa tcgacaccgc catctggggt
    16021 gaagacggca actttatcga tgccgtcgcc catatgatct tgcccgccat tgtgctgggt
    16081 actattccgc tagcggtcat tgtgcgtatg acacgctcct cgatgctgga agtgctgggc
    16141 gaggattaca tccgcaccgc gcgcgccaaa gggctaaccc gcatgcgggt gattatcgtc
    16201 catgcgctgc gtaacgcgat gctgccggta gtgaccgtta tcggcctcca ggtgggaaca
    16261 ttgctggcgg gggcgattct gaccgaaacc atcttctcgt ggcccggtct ggggcgctgg
    16321 ttgattgacg cactgcaacg ccgcgactat ccggtagtgc agggcggcgt attgctggtg
    16381 gcgacgatga ttatcctcgt caacttgctg gtcgatctgc tgtacggcgt ggtgaacccg
    16441 cgtattcgtc ataagaagta aggggacatc atgtcacagg ttactgaaaa taaagtgatt
    16501 agcgcaccgg tgccgatgac cccgttacag gagttctggc actattttaa acgcaacaaa
    16561 ggcgcggtcg tcgggctggt ttacgtcgcc atcgtgctgt tcatcgcgat ctttgccaac
    16621 tggattgcgc cttataaccc ggcggatcag ttccgcgatg cgctgctcgc cccgccagcc
    16681 tggcaggaag gcggcagtat ggcacacttg ctgggcaccg atgacgtcgg tcgtgatgtg
    16741 ctgtcgcggc tgatgtacgg cgcgcgttta tcgctgctgg ttggctgtct ggtggtggtg
    16801 ttgtcgctga ttatgggcgt tattctcggc ctgatcgccg gttacttcgg cggcctggtt
    16861 gataacatta tcatgcgcgt ggtcgatatc atgctggcgc tgccaagtct gctgttggca
    16921 ctggtgctgg tggcgatttt cggcccgtcg attggtaacg ccgcgctggc gctgaccttc
    16981 gttgccttgc cgcactatgt gcgcttaacc cgcgccgccg tgctggtgga ggtgaatcgc
    17041 gattacgtca ccgcctctcg cgtggcaggt gccggggcga tgcgccagat gtttattaac
    17101 atcttcccga actgccttgc gccgctgatt gttcaggcgt cgctcggttt ctctaacgcc
    17161 attctcgata tggctgctct tggtttcctc ggcatggggg cacagccgcc aacgcctgag
    17221 tggggcacca tgctctccga cgtgttgcag ttcgcgcaaa gcgcctggtg ggtcgtgacc
    17281 ttccctggtc tggcgatcct gttgacggtg ctggcattta acctgatggg tgacggtctg
    17341 cgtgacgcgc tcgatcccaa actaaagcag taagaggttc gagatggcgt tattaaatgt
    17401 agataaatta tcggtgcatt tcggcgacga aagcgcaccg ttccgcgccg tagaccgcat
    17461 cagctacagc gtaaaacagg gcgaagtggt cgggattgtg ggtgagtccg gctccggtaa
    17521 gtcggtcagt tcactggcga ttatggggct gattgattat ccgggccgcg taatggcaga
    17581 aaaactggag tttaacggcc aggatttgca gcgtatctca gaaaaagagc gccgcaacct
    17641 ggtgggtgcc gaagtggcga tgatcttcca ggacccgatg accagcctta acccgtgcta
    17701 caccgtgggt ttccagatta tggaagcgat taaagtgcat cagggcggca acaaaagtac
    17761 ccgccgtcag cgggcgattg acctgctgaa tcaggtcggt attcccgatc cggcttcgcg
    17821 tctggatgtt tacccgcatc agctttccgg cggcatgagc cagcgcgtga tgatcgccat
    17881 ggcgattgcc tgtcggccaa aactgctgat tgccgatgaa ccgaccaccg cgctggacgt
    17941 gaccattcag gcgcaaatca tcgaactact gctggagcta cagcagaaag agaacatggc
    18001 gctggtgtta attacccatg acctggcgct ggtggcggaa gctgcgcata aaatcattgt
    18061 gatgtatgca ggtcaggtag tagaaacggg cgatgcacac gccatcttcc atgcgccgcg
    18121 tcacccgtat actcaggcat tgctgcgtgc gctgccagaa tttgctcagg acaaagaacg
    18181 tctggcgtcg ttgccaggtg ttgttcccgg caagtatgac cgcccgaacg gctgcctgct
    18241 taacccgcgc tgcccctatg ccactgacag atgtcgcgct gaagaaccgg cgctgaatat
    18301 gctcgctgat gggcgtcagt ccaaatgcca ttacccactt gatgatgccg ggaggcccac
    18361 actatgagta cgcaagaggc cacctcgcaa caaccgctgt tgcaggctat cgacctgaaa
    18421 aaacattatc cggtgaagaa aggcatgttc gtgccggaac gtctggtgaa ggcgctggac
    18481 ggcgtttcgt ttaaccttga acgtggcaaa acgctggcag tagtgggcga atctggctgc
    18541 ggtaaatcga ccctcggtcg gttgctgacg atgatcgaaa cgcccaccgg cggcgagctg
    18601 tattatcagg ggcaggatct gctcaagcac gatccgcagg cgcagaagct gcgtcggcag
    18661 aaaatccaga tcgtcttcca gaatccctac ggttcgctga atccgcgtaa gaaagttggg
    18721 caaattctcg aagagccgct gcttattaac accagcttaa gcaaagaaca gcgtcgggaa
    18781 aaagccctgt cgatgatggc aaaagtcggc ctgaaaaccg agcactatga ccgctatccg
    18841 cacatgtttt ccggcggaca acgtcagcgt atcgctattg cccgtggtct gatgctcgac
    18901 ccggatgtgg tgattgccga tgagccggtt tctgcgctgg acgtctcagt gcgtgcgcag
    18961 gtgctgaatc tgatgatgga tttgcagcag gagttggggc tttcttatgt ctttatctcc
    19021 cacgacctgt cggttgtgga gcacattgct gatgaagtga tggtgatgta cctaggccgc
    19081 tgcgtggaga aaggaacgaa ggaccaaatc ttcaataacc cgcgccatcc gtacacccag
    19141 gcgctgctct ccgcgacgcc acgcctgaac ccggacgatc gtcgtgaacg catcaaactc
    19201 accggtgaac tgccaagccc gctgaatcct ccgccgggtt gcgccttcaa cgcccgctgt
    19261 cgtcggcgct tcggcccctg cacccagttg cagccgcagc taaaagacta cggaggtcaa
    19321 ctggtagctt gttttgctgt tgatcaggat gaaaatccgc agcgttaact cacaaaaccg
    19381 gggccatgcg ctccggttta ctcaatcagc ttcaacagtg gcgacaagca tagcaaaatg
    19441 ccgtaaagca gaatcagcca ggctttaaat ccgcgaagtt tttccagttg tgcgacttta
    19501 tagatcagga aaaacggaat gaggcacgac acaatgccat ataacgggct tcccaactga
    19561 aagaacacca gcactgatac acgaaacgaa acccaaatcg tcaacgtaat gacgataaaa
    19621 gcgcagatcg ccagagtcag catgcgtgag ttaattttct tggtatcaat aattcggctt
    19681 aacagattga gaataatgcc tttaatagcc tcgtggaaac cgagataaat gccaaagaat
    19741 gcggtcagta cggcaaagat attcagcacc gtagaggtga tatgaatgat atgtccaggg
    19801 atcacctgcg cggccagtgc cagtgccgag atattttgtt caaaggcaga aacggcttct
    19861 tcgtggctaa ttgagaaggt aaacgaaaag gcaaaaaaca ggatcaccgc gatgagcgtg
    19921 atataactaa ttcggtgggt acgcagcgcg agccgggttg ccagtacttt atctgcttcc
    19981 cgtttacgat aggcaatatt cattgggtta agtacctgaa taaataccgc agaaaagaag
    20041 caaaatggaa tggtaagcag gacatcgcgg aaaaagacgg aggcttgcgg gaaggcggtt
    20101 atattggcga aattccagtg cgggatcatc gcaaaaccga acacgacaat aatccctact
    20161 ttgaccacca ccattggccc agaaatctta aacagtaatc gttcaccacc agacgcaatc
    20221 gctatcagta cggcgaaaat agcgacttta taaagtagag attgtgaaag atcggcatcg
    20281 gttaaaccga aggttttcag gtacgaggcg ctgtcgaaaa ccacggagag agagtagata
    20341 aaaatcccgt ggataatcat caaaaagtag ataaccccga ggaaaattcc ccagttcttc
    20401 cccaggtaat gactgataat atcggtgtag tcattacagg aatcgctttc agaaagggtt
    20461 tttaaataaa tgtcctgcac tacccaggtg acaggataag caatgatcgc tgcggtaata
    20521 aatacccaaa ttcccttcaa gccaatttgt actggcatca gcacggttcc ggcaccaatc
    20581 gccatgccta tgcataataa aacccagccg aagtcgtagc gtgtaaacgg caatttctga
    20641 ttgtgtttcg atagtgtgtt gtgctgcata aagtcctgtc cagaaagaag tcggcctcgc
    20701 aaaaatgaat aaacccagtt tctcttgcgc aacaggtgga gaagtcgaat acgcagtatg
    20761 taaaaaatga ggcaacagat tcactgggga ggtcgcgagg aggcgcaatt ctacaaaatt
    20821 gcgcatttca ctaccagtct ggagtcagcc cggatatgaa ctgcaactat tgcaaataaa
    20881 gaaattaagc tggaaggcat cgcgcaatga ggaagattgc cgggtgctga cagtaaaaag
    20941 ca
//
LOCUS       DAJVPP010000071        20479 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVPP010000071 DAJVPP010000000
VERSION     DAJVPP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.071"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            53..407
                     /locus_tag="OQM34_002652"
                     /pseudo
     CDS             53..407
                     /locus_tag="OQM34_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            445..750
                     /locus_tag="OQM34_002653"
     CDS             445..750
                     /locus_tag="OQM34_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1453037.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            753..1157
                     /locus_tag="OQM34_002654"
     CDS             753..1157
                     /locus_tag="OQM34_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1453038.1"
                     /translation="MADTHHAQGPGKSFLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            1147..1446
                     /locus_tag="OQM34_002655"
     CDS             1147..1446
                     /locus_tag="OQM34_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1453039.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            1632..1928
                     /locus_tag="OQM34_002656"
     CDS             1632..1928
                     /locus_tag="OQM34_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1453040.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            1998..2984
                     /gene="yqjG"
                     /locus_tag="OQM34_002657"
     CDS             1998..2984
                     /gene="yqjG"
                     /locus_tag="OQM34_002657"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCS1453041.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            3277..3645
                     /locus_tag="OQM34_002658"
     CDS             3277..3645
                     /locus_tag="OQM34_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1453042.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(3852..4748)
                     /gene="yhaJ"
                     /locus_tag="OQM34_002659"
     CDS             complement(3852..4748)
                     /gene="yhaJ"
                     /locus_tag="OQM34_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCS1453043.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            4853..5554
                     /locus_tag="OQM34_002660"
     CDS             4853..5554
                     /locus_tag="OQM34_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1453044.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            5577..5741
                     /locus_tag="OQM34_002661"
     CDS             5577..5741
                     /locus_tag="OQM34_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453045.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(5953..7263)
                     /locus_tag="OQM34_002662"
     CDS             complement(5953..7263)
                     /locus_tag="OQM34_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1453046.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIASLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(7291..8622)
                     /locus_tag="OQM34_002663"
     CDS             complement(7291..8622)
                     /locus_tag="OQM34_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS1453047.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(8897..10261)
                     /gene="tdcG"
                     /locus_tag="OQM34_002664"
     CDS             complement(8897..10261)
                     /gene="tdcG"
                     /locus_tag="OQM34_002664"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1453048.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(10333..10722)
                     /locus_tag="OQM34_002665"
     CDS             complement(10333..10722)
                     /locus_tag="OQM34_002665"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1453049.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(10736..12925)
                     /gene="tdcE"
                     /locus_tag="OQM34_002666"
     CDS             complement(10736..12925)
                     /gene="tdcE"
                     /locus_tag="OQM34_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS1453050.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIACCVSP
                     MVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLD
                     HFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDSLSA
                     IKYARVKPIRDENGLAVDFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYRN
                     AVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKL
                     PFTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNR
                     EMLLDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL"
     gene            complement(12959..14167)
                     /gene="tdcD"
                     /locus_tag="OQM34_002667"
     CDS             complement(12959..14167)
                     /gene="tdcD"
                     /locus_tag="OQM34_002667"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1453051.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(14193..15524)
                     /gene="tdcC"
                     /locus_tag="OQM34_002668"
     CDS             complement(14193..15524)
                     /gene="tdcC"
                     /locus_tag="OQM34_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1453052.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(15546..16535)
                     /gene="tdcB"
                     /locus_tag="OQM34_002669"
     CDS             complement(15546..16535)
                     /gene="tdcB"
                     /locus_tag="OQM34_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1453053.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(16634..17572)
                     /gene="tdcA"
                     /locus_tag="OQM34_002670"
     CDS             complement(16634..17572)
                     /gene="tdcA"
                     /locus_tag="OQM34_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1453054.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            17762..18106
                     /gene="tdcR"
                     /locus_tag="OQM34_002671"
     CDS             17762..18106
                     /gene="tdcR"
                     /locus_tag="OQM34_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1453055.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            18362..18901
                     /locus_tag="OQM34_002672"
     CDS             18362..18901
                     /locus_tag="OQM34_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453056.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            18923..19270
                     /locus_tag="OQM34_002673"
     CDS             18923..19270
                     /locus_tag="OQM34_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453057.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            19439..20089
                     /locus_tag="OQM34_002674"
     CDS             19439..20089
                     /locus_tag="OQM34_002674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453058.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         5569 a         5180 c         4688 g         5042 t
ORIGIN      
        1 ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa gaatgaaata
       61 ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg ccactaccct
      121 gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa aacaccaaaa
      181 ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca actgttcaga
      241 tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg aggtggcgga
      301 acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa cggaaactgg
      361 cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc acaatagtca
      421 ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca tctgcgtgct
      481 gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg cgagaagtcg
      541 aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca gagccgttat
      601 cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc ggcgcgtgct
      661 gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc aatcggtgta
      721 gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac gcgcaagggc
      781 ccggtaaaag ctttctgggc atcgggcagc gaattgtttc catcatggtt gaaatggtgg
      841 aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat ctctttcaac
      901 ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg agcctgatgg
      961 tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt gccaccaccg
     1021 tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa tcgcgtaaat
     1081 ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg ctcgaggagg
     1141 agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct tagccagatc
     1201 cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc aacaggcgct
     1261 tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt tggcagtagc
     1321 gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc cagacgcggt
     1381 tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca acagcttcgc
     1441 ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt ttgaatattt
     1501 ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc tccatcgaca
     1561 gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg agtggcgcag
     1621 tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac attttaatgc
     1681 cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt acccaacaat
     1741 atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta tttcacagca
     1801 actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt tctggcggat
     1861 tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg ctgaataaaa
     1921 agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc tcgtttttgc
     1981 tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac acctggtacg
     2041 ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt aactggctca
     2101 ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag aaagatcgtt
     2161 atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc atgcgcaaac
     2221 tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg ctggaaaacg
     2281 gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat caacatgaat
     2341 ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt actgttcccg
     2401 tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa atcatccgca
     2461 tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac ccaccagccc
     2521 tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac aacggcgtgt
     2581 ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg aaagtgtttg
     2641 aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc ggcaaccagc
     2701 taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca gtgtatgtga
     2761 cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat ggcttcctgc
     2821 gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac atccgtaatc
     2881 attacttccg cagccataag accatcaacc ctacggggat tatttcaatt ggtccgtggc
     2941 aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag gcaccttacg
     3001 aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc gtgaacgcct
     3061 tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag gccagataag
     3121 cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc acggcacttt
     3181 attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt aatttgctgc
     3241 tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct gaaagtacta
     3301 aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat gtttattctg
     3361 gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg ctggcaacgc
     3421 gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt accgtggtgg
     3481 gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc actgctattc
     3541 ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc gggtacgcca
     3601 ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt aagcaccttg
     3661 taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg cggcgtaaac
     3721 gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc gtaggcctga
     3781 taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc ttgtgaaagg
     3841 tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca ccaggatttc
     3901 gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct ggtcgattcc
     3961 gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg atacggcatc
     4021 gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt
     4081 aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg agcggtatcc
     4141 gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc cggctcctga
     4201 tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag cttgcgcgag
     4261 ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc cgcccgcccc
     4321 tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag ttgggtattg
     4381 gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac cagcgcttcg
     4441 gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc agtagtcagt
     4501 ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc gacattggtg
     4561 aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc ttccagtttt
     4621 tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc cgccgcaaaa
     4681 ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa tgccctttct
     4741 ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat ctggctaaca
     4801 atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa ttatgattac
     4861 tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg cccggtatac
     4921 tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc tgcgtgtgct
     4981 taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc ccaaagtcga
     5041 tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag gcaatcatgt
     5101 tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca gctatagcga
     5161 acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg atacctgccc
     5221 gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc agcaattaat
     5281 cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt ggctgcacca
     5341 tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac gcgcgtattt
     5401 gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag cggcgctaac
     5461 ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg ccgattcccc
     5521 actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag atgatgatga
     5581 gtaaaaaatc gaccaaaaag cgccagccag caaagcccgt ggtggcgaaa gaacctgctc
     5641 gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct atcgtcgcgg
     5701 atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt gccggatgcg
     5761 gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa acaccccgta
     5821 ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca acaacatcaa
     5881 caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg cacaatactc
     5941 aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc gatccgtttg
     6001 ctgcatcgaa tggcttgcta acgcacacag gctggcaatc gactgctcaa catcatgcgc
     6061 cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca ccgctttcca
     6121 cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat
     6181 catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt cataacgccc
     6241 atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg cggcacaaag
     6301 tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag aaagcatcag
     6361 cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca ccacaggcat
     6421 tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa gcgtagcgcc
     6481 gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag agagatcttt
     6541 cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca gcccccactt
     6601 accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga gaataaagcg
     6661 gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg tcgttctgga
     6721 aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt gggtaaacac
     6781 cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga tggtgacaca
     6841 cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat tgcaaggttc
     6901 ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg caattgcctg
     6961 cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta acgcccccag
     7021 cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc ccagaccgtt
     7081 cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt ccagttctgc
     7141 cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca gcgccggttt
     7201 tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa
     7261 tatattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca gagaaacaca
     7321 aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt tgtgcaatgc
     7381 cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga aaatcgggct
     7441 acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc aggccagcaa
     7501 aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct cggcaggcat
     7561 cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac
     7621 gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca ctttcaccca
     7681 cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc
     7741 gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa
     7801 gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt tatgccgcgc
     7861 cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta acgtctggat
     7921 aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca ccagcagccc
     7981 cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg acacgcccag
     8041 cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga ataacaattt
     8101 ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat aaaagggact
     8161 gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac tatcgttggt
     8221 gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga gcgcacctaa
     8281 caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat ctttacattc
     8341 tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc
     8401 aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa
     8461 aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact
     8521 gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt
     8581 cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc gctcaatatt
     8641 ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc tacgtttttg
     8701 caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat cttcacaatc
     8761 aagactttaa aataacggag atggataaga attttctact aaattaatcg caagagaata
     8821 ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca cccaaggatg
     8881 aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg cgtgatgttt
     8941 cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc actttatcga
     9001 gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt actgctttca
     9061 cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca accggatcgc
     9121 aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc tgcgccggac
     9181 tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg ccaatctccc
     9241 cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc gcgccaatag
     9301 cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga cggaacttat
     9361 cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt gccgttacca
     9421 cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg atccagtcga
     9481 tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga cgcagcgcta
     9541 cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt
     9601 caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt tccgctttgc
     9661 tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg ttgtaatcac
     9721 acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt
     9781 gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag taataggttt
     9841 tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc tcatggcgag
     9901 gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca atatgcgcac
     9961 cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca gggatctcat
    10021 caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg acatccgtgg
    10081 catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata tggctcgtcg
    10141 cggttaataa gccgctactt tccagccgat caataaaact ttttccggca ttcattggcc
    10201 ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat gcactaatca
    10261 tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg cgaccgtccc
    10321 gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct tcacatcttt
    10381 cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa
    10441 gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga tgaacacggt
    10501 catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt tgacgttttc
    10561 gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct gcgggcaaac
    10621 cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa cataagggcc
    10681 gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc cggcgtcaga
    10741 gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc agtgcgttga
    10801 agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc gggtgctcga
    10861 tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga ccgccttcga
    10921 catccgcttc gtggtggaaa tacccatcca gcaggccaac aaggttggtt ttacgtactg
    10981 gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc ccatctttgg
    11041 cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct ttgcggtcac
    11101 gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg
    11161 tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc gactgggtag
    11221 ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt tcaaccaggt
    11281 cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga
    11341 tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg gcatatttga
    11401 tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat
    11461 agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt
    11521 agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg aagtgatcga
    11581 ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct gttttcggcc
    11641 cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg
    11701 ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt gggctgacgc
    11761 agcaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
    11821 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
    11881 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
    11941 caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca
    12001 gttcctgtgc ctgctgctca ttgaggacgc cagctttaaa gtcgcgctca atgtagatat
    12061 ccaggaacga tgccgtgcgc cccagcgaca tcgcgccgcc attttgcgat ttcactgccg
    12121 ccagataagc gaagtagagc cactgcaccg cttcctgtgc attctgcgcc gggcgagaga
    12181 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
    12241 ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact
    12301 ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca tacagcgcta
    12361 cgcggcgata gtcaccgata atgcgcccac gaccatagcc atctggtaaa ccggtcagca
    12421 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
    12481 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cggccatagg
    12541 cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg
    12601 gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat
    12661 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
    12721 cgatacggat gccttccatt accttttccc acaattccgt ggtggcaggc gtcgcttcgg
    12781 cgagaaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
    12841 taatttcgct tttccagtcc gtacctttaa agccaagcca tgcgtcagcg tacagcttat
    12901 cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt
    12961 atgcaaattc tgcgggcgcg ttaactttac ctaaatgaat ggcatccaaa gcaatcattt
    13021 tttcttcgtt agtcggaata acgacgcaaa tgacatgcgc attttcactg gaaacaattc
    13081 gctcgccaaa agagttagat cgattattca tttctgtatc aatctctacg cctaatacag
    13141 ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt ccgccggtga
    13201 atataattcc atccagccga cgtaatgaag ctgcatgtcc ggcaatatga cgggcaattc
    13261 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
    13321 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
    13381 ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac
    13441 caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca
    13501 ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat
    13561 gcgccacaac caggccggag tcatcttccg ccagattcag cagcgaatgt gcgcgctggg
    13621 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
    13681 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
    13741 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
    13801 aatttgcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt
    13861 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
    13921 ttaaggccac gctgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
    13981 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
    14041 cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacaa tcgctggcat
    14101 cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
    14161 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
    14221 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
    14281 agagacggcg ctttacggat agcatacatc ggcaacaggc acagcaggga tgcgataatc
    14341 ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc aacaacccag
    14401 gtggagccca tgatgaagat catgctgaga gtattcagtt tacccagcga cactttggtt
    14461 ttgtcacctt tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga
    14521 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
    14581 agtgtaatcg cgaacgttgt tttcgtaccg gtcatggacg caaagtggtt agccagataa
    14641 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagagtaaac
    14701 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
    14761 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
    14821 ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa
    14881 atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
    14941 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
    15001 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
    15061 aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc
    15121 ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
    15181 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
    15241 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
    15301 agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
    15361 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
    15421 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
    15481 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
    15541 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
    15601 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
    15661 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
    15721 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
    15781 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
    15841 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
    15901 ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
    15961 ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac atcatagaga
    16021 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
    16081 ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
    16141 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
    16201 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
    16261 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
    16321 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
    16381 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
    16441 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca
    16501 tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
    16561 atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
    16621 taaaaacaaa caactaacca acttctatta attgccttcg tctacaccca ttataagatg
    16681 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
    16741 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
    16801 gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
    16861 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
    16921 catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca
    16981 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
    17041 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
    17101 tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
    17161 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
    17221 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
    17281 ccaccaccgc ttcagaagac ataccgctta tctcattaac catatttttc atttcacggg
    17341 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
    17401 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
    17461 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
    17521 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
    17581 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaataaaca ggatatgtgc
    17641 gaccactcac aaattaactt tcattacttt ccaaggtatc gttattacaa ttaaatatct
    17701 ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt atcaaaaaaa
    17761 aatgacgggg ataaccatat tttatggcga taacatcatt cgttatgtgg ttaatacaaa
    17821 aaaggggctg agaccatatt tcaagcaatt accggataat taccaggcaa aatttgagtt
    17881 aaatttaatg agcaagttct caaattttat tataaataaa ccattttcag cgataaatac
    17941 tgcggcacgt cacatttttt cacgctattt gttggagaac aaacatttat tttatcaata
    18001 ttttaaaatt tcgaatacag gtattgatca tctcgaacaa ttgattaacg tcaacttttt
    18061 ctcttctgac aggacgtcat tttgtgaatg caatcgtttt ccataaattc ttctcccctc
    18121 ataggcgacg aatagcattt tgtgttgagg atcacaaaac gaataattgc tggtcgccgc
    18181 gataaggtca gacgaagtca acaaggggga ttttcactgt gctttacatc agcgtaggac
    18241 acagaatgtt gcttcgttta ctgtaacgcc gggtaaatga gcgttttttg atagtgcgta
    18301 agtgtcgtgc gaggaaaata agcatctatt attgtttgtc atgacgacaa ggcaggcatc
    18361 aatgaaaggc ttcccaattg cacatatttt tcacccttca atcccgccaa tgcacgcagt
    18421 ggttaacaat cacaatagaa atattgatta ttggacggta aaaagaaagt ttgcagaaat
    18481 tgtctccacc aatgacgtta ataaaattta cagtataagt aatgaactcc ggagagtatt
    18541 atctgcaatt actgcattga atttctatca aggcgatgtt ccttctgtca tgatccgaat
    18601 ccaaccggaa aatatgagtc cattcatcat agatatttct acaggagaac atgatgatta
    18661 tatcatacaa acattagatg taggcacttt tgcacctttt ggtgaacaat gtacttgctc
    18721 agccgtcaat aaaaaagagc tggaatgtat taaggagacg atttctaagt attgcgcaaa
    18781 gttcacccaa aaagaagcca tcttaacacc ccctaggcac tttaataaaa cctgcattac
    18841 ttcagattgt tggcaaattc tctttttttc acccgatcat tttaataatg atttttattg
    18901 attatttata aaggataaaa taatgttccc tgtttcatca attggtaatg atattagtag
    18961 tgatttagtc cgtcgaaaaa tgaatgacct tcctgaaagc cccataggaa ataacctcga
    19021 agcactagca ccaggtatag aaaaactaaa acagacctct attcaaatgg tcactttact
    19081 taacgcgtta caaccaagtg gaaaatgcat tatcactggt gattttcaaa aagaattagc
    19141 gtacttacaa aatgtaattc tttataatga ctcgtctctt cgtctggatt ttttaggtta
    19201 taacacccaa attattcaac gatcggacaa tacctgcgaa cttaccatta atgaaccgtt
    19261 aaaaaactag gaaatatcta caggcaatat caatgttaat tgcccattaa aagatattta
    19321 caatgaaatc aggaggttga acgtaatctt tagttgtggg actggagata tagttgatct
    19381 atcctctctg gacttacgta atgtcgattt agattattat gatttcacag ataaacatat
    19441 ggctaatact attctaaatc attttaaatt ggattcaaca aattttacta atgccaacat
    19501 gtttcaggtt aattttgtta gttcaacaca aaacgccaca atctcctggg attatttact
    19561 aaaaataacg cctgttttaa taagcattag cgatatgtat tctgaagaaa aaatcaagtt
    19621 tgtcgaaagt tgtttaaatg agcttggaga cattaccgaa gaacaattaa aaattatgag
    19681 atttgcaatt ataaaatcta taccaagggc aactcttaca gataaattag aaaatgaatt
    19741 aacaaaagaa atatataaaa gctcatcgaa aatcaacaat tgcttgaaca gaattaaatt
    19801 accagagatg aaagaattct catcaaaaaa aatatatgat tacatcgata caatcattga
    19861 agattatgaa aatatcaaag aaaatgctta tctggtcgtc ccccaaatta attatactat
    19921 ggatttaaac atagaagact ctagctcaga agagttaata ccctcgagaa agacgaaaat
    19981 tctccggaca atggctttga ggtcggggaa tataacacat atgaagcata taactcagag
    20041 aatcaatatt ttaccagaga ggactatacg tataattacg accttttaaa tgcaatatag
    20101 acttaataac ttcttacaaa gtctattatt aataccgtag atattcatct taccctgtgc
    20161 accaaaagca aaaacttatc gacgcgggtt tgaaactgat ggcaaccgca aaaatgcctg
    20221 atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg cgtgcttttg
    20281 taggcaggat aaggcgttca cgcagcatcc ggcatgaata aagcgcactc aacaatctga
    20341 aaccccccgg aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt
    20401 ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc
    20461 cgaagcgggt ttttacgta
//
LOCUS       DAJVPP010000072        20368 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVPP010000072 DAJVPP010000000
VERSION     DAJVPP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.072"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..196
                     /locus_tag="OQM34_002675"
     CDS             <1..196
                     /locus_tag="OQM34_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCS1453059.1"
                     /translation="GWSPGTELTVEKKGDSVNLRATEHKPRGRLTVAQLLSQIDESEI
                     TELNQSTEGWAEGKKGNEAW"
     gene            190..555
                     /locus_tag="OQM34_002676"
     CDS             190..555
                     /locus_tag="OQM34_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1453060.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(556..1344)
                     /gene="bioH"
                     /locus_tag="OQM34_002677"
     CDS             complement(556..1344)
                     /gene="bioH"
                     /locus_tag="OQM34_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1453061.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1382..2065
                     /gene="gntX"
                     /locus_tag="OQM34_002678"
     CDS             1382..2065
                     /gene="gntX"
                     /locus_tag="OQM34_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1453062.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2124..2699
                     /gene="nfuA"
                     /locus_tag="OQM34_002679"
     CDS             2124..2699
                     /gene="nfuA"
                     /locus_tag="OQM34_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1453063.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3060..4376
                     /gene="gntT"
                     /locus_tag="OQM34_002680"
     CDS             3060..4376
                     /gene="gntT"
                     /locus_tag="OQM34_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1453064.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4421..6505)
                     /gene="malQ"
                     /locus_tag="OQM34_002681"
     CDS             complement(4421..6505)
                     /gene="malQ"
                     /locus_tag="OQM34_002681"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1453065.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVSNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6515..8908)
                     /gene="malP"
                     /locus_tag="OQM34_002682"
     CDS             complement(6515..8908)
                     /gene="malP"
                     /locus_tag="OQM34_002682"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1453066.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9520..12225
                     /gene="malT"
                     /locus_tag="OQM34_002683"
                     /pseudo
     CDS             9520..12225
                     /gene="malT"
                     /locus_tag="OQM34_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12268..13284)
                     /gene="rtcA"
                     /locus_tag="OQM34_002684"
     CDS             complement(12268..13284)
                     /gene="rtcA"
                     /locus_tag="OQM34_002684"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS1453067.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13288..14514)
                     /gene="rtcB"
                     /locus_tag="OQM34_002685"
     CDS             complement(13288..14514)
                     /gene="rtcB"
                     /locus_tag="OQM34_002685"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS1453068.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14703..16301
                     /gene="rtcR"
                     /locus_tag="OQM34_002686"
     CDS             14703..16301
                     /gene="rtcR"
                     /locus_tag="OQM34_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCS1453069.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16283..17041)
                     /locus_tag="OQM34_002687"
     CDS             complement(16283..17041)
                     /locus_tag="OQM34_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1453070.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17058..17888)
                     /gene="glpG"
                     /locus_tag="OQM34_002688"
     CDS             complement(17058..17888)
                     /gene="glpG"
                     /locus_tag="OQM34_002688"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1453071.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(17933..18259)
                     /gene="glpE"
                     /locus_tag="OQM34_002689"
     CDS             complement(17933..18259)
                     /gene="glpE"
                     /locus_tag="OQM34_002689"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1453072.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18449..19954
                     /gene="glpD"
                     /locus_tag="OQM34_002690"
     CDS             18449..19954
                     /gene="glpD"
                     /locus_tag="OQM34_002690"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1453073.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20160..>20368)
                     /locus_tag="OQM34_002691"
     CDS             complement(20160..>20368)
                     /locus_tag="OQM34_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453074.1"
                     /translation="AAVIAMLGAGLAAIPEVCNLLFTPTIYLLLNIFMLTLGALMLFF
                     SGRVGNVPEFCYVGYDGVGFAVIP"
BASE COUNT         4797 a         5616 c         5152 g         4803 t
ORIGIN      
        1 tggctggtca ccgggcacag agcttaccgt ggagaaaaaa ggcgatagcg ttaatttgcg
       61 cgccacagag cacaaaccgc gtgggcgtct taccgtcgcg cagttgttga gccaaatcga
      121 tgaaagcgaa attacggagc taaatcagtc cacagaagga tgggctgaag ggaaaaaagg
      181 taatgaagca tggtaaaggc acggacgcca catcgtggtg agatctggta ttttaaccct
      241 gatccggttg ccgggcatga acttcagggg ccacattatt gcattgtggt aacggacaaa
      301 aaactcaaca atgttttaaa agttgctatg tgctgcccga tttcaacagg ggcaaatgca
      361 gcacgttcca caggggtgac ggtgaacgtc ctcccccgtg atacgcaaac cggtaacctg
      421 catggcgttg tactttgtca ccagctaaaa gccgtcgatc ttattgcccg tggcgctaaa
      481 tttcataccg ttgccgatga aaaattgatt agtgaagtta tcagtaaact ggtgaattta
      541 atcgacccac aataattagc tctcacttac caccaacacc ctctgcttca acgccaccag
      601 caggtgacaa aactcgaccg gatgcgaaat aaacggcgca tgggccgctt tggcgaagat
      661 atatgattcg ctgtgaggcc aaagtttatc cagcatcggc accactttgc gcggcaccag
      721 accgtcgaga tagccataca atcgcaaaaa cggcatggac acgttttgca gcggctgacg
      781 gagatcgacc gttttcagga tttccagccc gccattaagc acgtcaacct ccggcatcgg
      841 taacgccaga acggttttct tcaacgcccg cgcatcctgg cgcgccgttt cagtccccat
      901 ggtttgtaac gccaggaacc gctccactgt acgctgaaaa tcatcactga gttgctgctg
      961 aaatcccgcc agcacgtccg gttttatccc cggccactcg tcacgagcac taaaacaagg
     1021 tgacgacgcc acggtgacca gcgcctgaac acgctcggga tgggttaacg caatctggct
     1081 tgccaccagc ccgcccagac tccagcctaa ccaaatggct ttatcaggtg cctgttgcag
     1141 cacggcttcg gccatatcag caagtgacag cgcaccaaat ccccggctac gcccgaagcc
     1201 gggcaggtca acaaggtgca gcgtaaaatg cgagctaagt tcctcgtcaa tgcaacgcca
     1261 cacttcggca ttcagtcccc atccgtgcag cagcacaaga tgaacattcc cctgaccttt
     1321 ggtctgccac cagatgttat tcatccgcta ttgttctctt ttgacttaca aggatgaaca
     1381 tatgctaaca gtaccgggat tatgctggct atgccgaatg ccactggcgt taggtcattg
     1441 ggggatttgt tcggtctgtt cacgcgccac ccgcacagat aaaacgttat gcccacaatg
     1501 tggattaccc gccacacact cccatcttcc ctgcggtcgc tgcctgcaaa aaccgccgcc
     1561 ctggcaaaga ctggtcacgg ttgccgacta tgcgccgccg ttaagtccgc ttatccacca
     1621 gcttaaattt tcccggcgca gtgaaatcgc cagcgccctg tcacgtctgt tactattgga
     1681 agtcttacac gctcgtcgca ccaccgggtt gcaattgccg gatcgcatca tcagcgttcc
     1741 gttatggcag cggcgtcact ggcgtcgggg atttaatcag agcgatttgc tgtgtcagcc
     1801 gttatcacgc tggttgcact gccaatggga tagcgaagcc gtcacacgta cacgggccac
     1861 tgcgacccag cattttctca gtgcccggct gcgcaagcgc aacctgaaaa atgcctttcg
     1921 tcttgaattg cccgtgcaag gtcgccatat ggtgattgtg gatgatgtcg ttaccaccgg
     1981 aagtaccgtc gcagagattg cgcagttgct tttacgcaat ggtgcggcga ctgtccaggt
     2041 ctggtgcctt tgtcgaacct tgtagagcct cgatgatggg cgtattataa ccaactaaaa
     2101 tagtcaacta ttaggccatt actatgatcc gtatttccga tgctgcacaa gcgcactttg
     2161 ccaaactgct ggcaaatcag gaagaaggga cacaaatccg cgtatttgtg attaaccctg
     2221 gcacgcctaa cgctgaatgt ggtgtttctt attgtccgcc ggatgccgtg gaagccaccg
     2281 acactgccct gaaatttgac ctgctgaccg cgtatgttga tgagttaagc gcaccatacc
     2341 tggaagatgc agagatcgac tttgttaccg accagttggg ttctcagtta acgctgaaag
     2401 ccccgaacgc caaaatgcgt aaagtggcag acgatgcacc gctgatggag cgcgtggagt
     2461 atatgctgca gtcgcagatc aacccacagc ttgctggtca cggtggtcgc gtttcgctga
     2521 tggaaatcac cgaagacggt tacgccattc tgcaatttgg cggcggctgt aacggttgtt
     2581 ccatggtcga tgtgacgctg aaagaaggga tcgagaagca gctgctgaac gaattcccgg
     2641 agctgaaagg tgtgcgcgat ctcaccgaac accagcgcgg cgaacactcc tactactaag
     2701 ttatcccctc ttttgtagat tgcccgatgc gacgctaaag cgtcatttcg ggcctacgtt
     2761 gaacatgtgc cagacgttgg cacagcattg ctttggcaaa tccctccctt cgtttccccg
     2821 cgataatatg accaacctct cataatttaa atttaccccg ctctggtgat tctcaaacgc
     2881 caaatgttac ccgtatcatt cacatgggta ccaaacatac tcctgacatc tgactacaat
     2941 aattagtttt agtgggtatc agtcgtggtg ccgcaatatc tctgttcccg attgggataa
     3001 ttagagtttg tcgtcagaaa attgacgtta cccataacaa atgaaaggcc aggtaaatca
     3061 tgccattagt cattgttgct atcggtgtaa tcttgttgtt gctcctgatg atccgcttca
     3121 aaatgaacgg cttcatcgct ctcgtcctcg tggcgcttgc tgttggatta atgcaaggaa
     3181 tgccgctgga taaagttatt ggctccatca aagccggtgt cggcgggacg ctcggtagcc
     3241 ttgccctgat catgggtttt ggcgcaatgc tgggcaaaat gctggcagac tgcggtggcg
     3301 cacaacgtat cgccaccacg ctgattgcca aatttggtaa aaagcacatc cagtgggcgg
     3361 tggtgttaac cggttttacc gttggttttg ccctgttcta tgaagtgggc ttcgtgctga
     3421 tgctgccgct ggtgtttacc atcgcggctt ccgcgaatat tccattgctg tatgttggtg
     3481 taccaatggc ggctgcactg tctgtgaccc acggcttcct gccaccgcat ccgggcccga
     3541 ctgcgattgc caccattttc aatgccgata tgggtaaaac cctgctgtac ggtactattc
     3601 tggcaatccc gaccgtgatt ctcgccggtc cggtttacgc tcgcgtgttg aaaggtatcg
     3661 ataagccaat cccggaaggt ctctacagcg cgaaaacctt cagcgaagaa gagatgccga
     3721 gctttggcgt cagcgtctgg acctctctgg tgccggttgt gctgatggcg atgcgtgcga
     3781 ttgccgaaat gatcctgccg aaaggtcacg ctttcctgcc ggtagcggag ttcctcggtg
     3841 acccggtaat ggcaacgctg attgccgtgc tgattgcgat gttcaccttt ggtctgaacc
     3901 gtggtcgttc aatggatcag attaacgaca cgctggtttc ttccatcaaa atcattgcga
     3961 tgatgctgtt gatcatcggt ggtggcggtg cgttcaagca ggtgctggta gacagcggcg
     4021 tggacaaata cattgcttcc atgatgcacg aaaccaacat ttctccgctg ctgatggcct
     4081 ggtcgattgc tgccgtactg cgtatcgcgc tgggttccgc aaccgttgcg gcaatcactg
     4141 cgggtggtat cgcggcaccg ctgattgcaa cgacgggtgt tagcccagag ctgatggtta
     4201 ttgcggtggg ttccggtagt gtgattttct ctcatgtgaa cgatccgggc ttctggctgt
     4261 tcaaagagta ctttaacctg actatcggcg agaccatcaa atcctggtcg atgctggaaa
     4321 ccattatctc ggtgtgcggt ctggtaggct gtctgctgct gaatatggtg atttgataac
     4381 actgactgcc ggatgcatcg catccgacat caaccgcact ctacttcttc ttcgctgcag
     4441 ctctgcgccg tctgtccaaa tccttcagca acttgttcac gccatcatcg gcaaacatcg
     4501 actcaagcgt tgcggaaagc ttgcgtcgcc agtttttata ctggtaactg gtgccaggaa
     4561 tattcaccgg ttcggccata tccagccagt cttccggctg tagtcctaac agagcactgt
     4621 tactgtcggc aatgtagcgc tgcaaaccac ggttcagcgt cggcgtcatc gacatcaacg
     4681 atgccttatg cccggcacgt tttggcagac aaccatattt atgcagtgca tccagcagcc
     4741 cttgcttcgc cagttcgcga tcctgataca gaccgcgcag taccacttca tccggataca
     4801 gccccagggt tttgcccagc gttagatccc cgctctccca gtaaccacgc agcgttggca
     4861 ggtcatgtgt cgccgcaacc gccatcgact gctccggata cgctttcggt gcacggaacg
     4921 tcttctcgtg gtcgttttcg aaatagagca ctttgtaaga gtacacaccg ctgctgcgca
     4981 gcttaccaac aatctctacc ggtacggtac cgagatcttc accaatcacc atacagcgat
     5041 gacgtttact ttcgagtgcc agaatcgaga gcagatcatc caccggatag tgaacatacg
     5101 cgccctgatc tgccgtctcg ccatacggta tccaccacaa acgcagcatc gacatcacat
     5161 ggtcaattcg taatgcgccg cagttttgca tattggcacg caacagctcg ataaacggtt
     5221 cataggcacg cgcggtgatg atatgcgggt ccattggcgg taatccccag ttctgcccca
     5281 acgggccgag gatatccggc ggcgcgccaa ccgatgcttt caggcaatat agttcacggt
     5341 cacaccaggt ttccgcccca ccttccgcta cgccaaccgc cagatcacga tacaagccaa
     5401 tcggcatttc atagccctgg cttatctccc agcaggcggc aaactggctg taagccagcc
     5461 actgcaacca gagataaaaa tcgacgtcat cacgatgttc ttcgcagaac tgacgcactt
     5521 ctggtgaatc cacgttctta tacatctctg gccatgcagg ccagccccag cgcatttcgt
     5581 cctctttcac ttgctgggca tgtagcgcat caaaggcagc ctgccagaac aggctgtcgc
     5641 cctgctctgc aacaaactgg cgaaacgcgg ccatctgctc atcatcacgt tgcgcgaaac
     5701 ctttccacgc cattcgtaat gctgtcattt ttagggcggt aaccgtggag taatcgaccc
     5761 agtcggcatc gcgcgcctgt tgcagcgtct gttgcgtggt cggcaactgc caccaggcct
     5821 gagcctcttc gctaagatgg aaatcttcaa cggcgttaac gtcgatataa atcacattca
     5881 gccaacggcg agaagacggg ctgtatgggc tggcgctctc cggatttgcc ggatagagcg
     5941 catgaatcgg gttcaggcca atgaacgacc cgccacgttt tgccacatcc accagcatcg
     6001 ctttgagatc gccaaaatcc ccaatacccc agtttttttc cgatcgcagc gtataaagct
     6061 gaacgcaggc accccacagc ttttgtttat tcagcaaagc ctgcggttcg taacaacgtt
     6121 tcggggcgac aatcacccgg caatgcgcgc gctggtcgtc ctgggtgagt gttagcgtgt
     6181 gataaccttc cggcagcttc gtcggtagat tgaacgcttt gccccccgtt acatggcctt
     6241 tgtactgcgt tccttcttcg gtggtcagct gccagctata ttcgccgctg ccctccacca
     6301 ccatcggcat ttttttgccg ctggtataaa ccatgacatt cgagactggc gttaccgcca
     6361 ctttcgtggc ggtacgttga tgcatcgcgt caagcaaacg ccgtttggtt tcggcgctaa
     6421 tcgactgcgg tttaccgtgg gcattgatgt aattggggct aatccccgcc gccagcgcgg
     6481 cattatccag acgtttgctt tccatcgagc ttccttagcg ttttgcctgc cagatacgag
     6541 cctgataatc gcgaatagag cgatccgagc taaacatacc gcagcgggcg gtattgagga
     6601 tcgccgcgcg agtccaggcc tcctggtcgc ggtacagcac atccacctgc ttttgtgcct
     6661 caacataggc tgcgaaatcc gccatcacca gatacggatc gccgccctgt ttgccgatac
     6721 tgtgcagcat ctggtcgaag gcatgcttat cgccgtcgct gtatttaccg ctttccagct
     6781 ctttcaatac tgcgtccagc accttatctt tcttccgcca tttcaccggg tcgtagcctt
     6841 tggccagaat agccttcact tgttccacgg tatgaccaaa aataaagata ttttcttcac
     6901 cgactttctc ggcgatttca acgttcgcac catccagcgt accgacagta agcgcaccat
     6961 tgagcgccag tttcatattg ccggtaccgg aagcttcttt acctgcagtc gaaatttgtt
     7021 cggagatatc cgccgccggg atcagttttt ccgccgccga aacgcaataa tccggcagga
     7081 acaccacctt caacttatcg ccaaccagtg gatcgttgtt gatcacgtca gccactttgt
     7141 tgatcgcaaa gataatattc ttcgccaggt agtagcccgg tgccgctttc gcgccgaaga
     7201 ggaagacgcg cggtacgcga tcagcctgca ggttttcacg aatttctttg tacaacgcca
     7261 gaatatgcag cagattcagg tgctggcgtt tgtactcatg cagacgtttg atctgaatat
     7321 cgaaaatcgc ctgtggattg atctcaatac cggtacgaac tttcacaaac tccgccagac
     7381 agactttatt cgcctgcttg atctcgcgat attgctgacg gaatttcgca tcatcagcga
     7441 atttttccag attgatcagc tgatcgagat cgttagccca ctctttttgc agtgatttat
     7501 ccaacagagc cgccagtgca gggttgcact gtttgatcca gcgacgtggg gtaataccgt
     7561 tggtaacgtt atggaatttg ttcggccata gctggtgata ttccgggaac agatctttca
     7621 ccaccagatc cgagtgcagc gccgcaacac cgttcaccgc gaaaccgcca accacacaca
     7681 ggttcgccat atgcacttgt ttatcgtgca ccaccgccag tttggcccac actttttcat
     7741 cgcccggcca ggttttctcc accagcgttt taaagcgagt attaatttcg ttaataatct
     7801 gcatatggcg cggcagtaag cctttcacca gtttcacatc ccagcgttcc agcgcttctg
     7861 gcatcagggt atggttggtg taagcgaaag ttttgctggt gatggcccag gcgtcatccc
     7921 agctcatctg gtgctcatcg gtcagcacgc gcagcagttc tggaatcgcg atagttggat
     7981 gggtatcgtt cagctgaata acttcgtaat ccgccagttc gtgcagttta cgccccgcca
     8041 gatgatggcg acgcaaaata tccgctaccg aacaggcaca ctggaagtat tgctgcatca
     8101 ggcgcagctt tttaccggca gtatggttgt cgtttggata gagaactttg gtcagttttt
     8161 ccgcattgat gccttgctgt tcggcgcgca ggaaatcacc gtcgttaaat ttagttagat
     8221 caaacggatg cgcatgcgtc gcctgccaca gacgcagcgg ctgcgccacg ccattacgat
     8281 agccgacaac ggggagatcc cacgcttgac cggtaatggt aaactccggc tcccagcgtc
     8341 cgtctttcat cactttaccg ccaatcccta cctgcacatc cagtgcttcg ttgtggcgga
     8401 accacgggta gttactgcga tgccagtcat ccggcgcttc aacctgtttg ccatcgacaa
     8461 aagactggcg gaacaaacca tattgatagt tcagaccgta acccgtcgca gactgaccga
     8521 cagttgccat tgagtcgagg aagcacgccg ccagacgtcc cagaccaccg ttacccagcg
     8581 ccgggtcgat ctcttcttcc agcaggtcag tcaggttgat gtcataagcc ttcaacgaat
     8641 cctgtacatc ctgataccag ccgagattca acaggttgtt gcccgtcaga cgaccaatca
     8701 aaaactccat tgagatgtag ttaacatgtc gctgattcgc caccggtttg gcgaatggct
     8761 gagcacgcag catttcggcc agtgcttcac tcactgccag ccaccactgg cgaggagtca
     8821 tttcagccgc agaatttaag ccataacgct gccactgacg tgaaagcgct tcctgaaatt
     8881 gcttatcgtt aaaaataggt tgtgacatag gagttccact tttcttagat tttcaacaca
     8941 acgttatcgc tagtttgcca ggctcgatgt tgaccttcct catcctgcgg gggattaggc
     9001 agggaggagt tgcggggatg agcaaggaaa tgtgatctcg accacttaaa gctagtgcaa
     9061 accacaggat tagcagcaaa tcaatgccat acagcgcaga aaatctgtat ctaagtgcaa
     9121 aaaatggccg ttgcgtattt tcaaaaagcg gaaggtaact ctataaatta agtaaaggag
     9181 tgaaacagtt tcacaagtaa aatatccagt gtgctccatc tcattcttaa tagatttatt
     9241 aagatcatct ttttagatgg cactttcatc aggaatgaag aagaaaccct tgcttaaatg
     9301 aatctgatga acataaggga aaccagtatt cacgctggat cagcgtcgtt ttaggtgagt
     9361 tgttaataaa gatttggaat tgtgacacag tgcaaattca gacacataaa aaaacgtcat
     9421 cgcttgcatt agaaaggttt ctggccgacc ttataaccat taattacgaa gcgcaaaaaa
     9481 aataatattt cctcattttc cacagtgaag tgattaacta tgctgattcc gtcaaaacta
     9541 agtcgtccgg ttcgactcga ccataccgtg gttcgtgagc gcctgctggc taaactttcc
     9601 ggcgcgaaca acttccggct ggcgctgatc acgagtcctg cgggctacgg aaagaccacc
     9661 ctcatttccc agtgggcggc aggcaaaaac gatatcggct ggtactcgct ggatgaaggt
     9721 gataaccagc aagagcgttt cgccagctat ctcattgccg ccgtgcagca ggcaaccaac
     9781 ggtcactgtg cgatatgtga gacgatggag caaaaacggc aatatgccag cctgacgtca
     9841 ctcttcgccc agcttttcat tgagctggcg gaatggcata gcccacttta tctggtcatc
     9901 gatgactatc atctgatcac taatccagtg atccacgagt caatgcgctt ctttattcgc
     9961 catcaaccag aaaatctcac cctggtggtg ttgtcacgca accttccgca actgggcatt
    10021 gccaatctgc gtgttcgtga ccaactgctg gaaattggca gtcagcaact ggcatttacc
    10081 catcaggaag cgaagcagtt ttttgattgc cgtctgtcat cgccgattga agccgcagaa
    10141 agcagtcgga tttgcgatga cgtttccggt tgggcgacgg cactatagct aatcgccctc
    10201 tccgcccggc agaataccca ctcagcccat aagtcggcac gccgcctggc gggaatcaat
    10261 gccagccatc tttcggatta tctggtcgat gaggttttgg ataacgtcga tctcgcaacg
    10321 cgccattttc tgttgaaaag cgccattttg cgctcaatga acgatgccct catcacccgt
    10381 gtgaccggcg aagaaaacgg gcaaatgcgt ctcgaagaga ttgagcgtca ggggctgttt
    10441 ttacagcgga tggatgatac cggcgagtgg ttctgctatc acccgctgtt tggcaacttc
    10501 ctgcgccagc gctgccagtg ggaactggcg gcggagctgc cggaaatcca ccgtgccgcc
    10561 gcagaaagct ggatggccca gggatttccc agcgaagcga ttcatcatgc gctggcggca
    10621 ggcgatgcgc tgatgctgcg cgatattctg cttaatcacg cctggagttt gttcaaccat
    10681 agcgaactgt cgctgctgga agagtcgctt aaggccctgc cgtgggacag tttgctggaa
    10741 aatccgcagt tagtgttatt gcaggcgtgg ctaatgcaaa gccaacatcg ctatggcgaa
    10801 gttaacaccc tgctagcccg tgctgaacat gaaatcaagg acatcagaga aggcaccatg
    10861 catgcagaat ttaacgctct gcgcgcccag gtggcgatta acgatggtaa tcaggatgaa
    10921 gcggaacggc tggcaaaact ggcactggaa gagctgccgc cgggctggtt ctatagccgc
    10981 attgtggcaa cctcggtgct gggtgaagtg ctgcactgca aaggcgaatt gacccgctca
    11041 ctggcgctag tgcagcaaac cgaacagatg gcacgccagc acgacgtctg gcactacgcc
    11101 ttatggagtt taattcagca aagtgaaatt ctgtttgccc aagggttcct gcaaaccgcg
    11161 tgggaaacgc aggaaaaagc gttccagctg atcaacgagc agcatctgga acagctgcca
    11221 atgcatgagt ttctggtgcg catccgagca caactgttat gggcctgggc acggctggat
    11281 gaagccgaag cgtcggcgcg tagcgggatt gaagtcttgt cgtcttatca gccacagcaa
    11341 cagcttcagt gcctggcaat gttgattcaa tgctcgctgg cccgtggtga tttagataac
    11401 gcccgtagcc agttgaaccg tctggaaaac ctgctgggga atggcaaata tcacagcgac
    11461 tggatctcta acgccaacaa agtccgggtg atttactggc aaatgaccgg cgataaagcc
    11521 gccgctgcca actggttgcg tcatacggct aaaccggagt ttgcgaacaa ccacttcctg
    11581 caaggtcaat ggcgcaacat tgcccgcgca caaatcttgc tgggcgagtt tgaacctgcg
    11641 gaaattgttc tcgaagaact caatgaaaat gcccgcagtc tgcggttgat gagcgatctc
    11701 aaccgtaacc tgttgctgct taatcaactg tactggcagg ccggacgtaa aagtgacgcc
    11761 cagcgcgtgt tgctggacgc attaaaactg gcgaatcgca ccggatttat cagtcatttt
    11821 gtcatcgaag gcgaagcgat ggcgcaacaa ctgcgccagc tgattcaact taatacgctg
    11881 ccggaactgg aacagcatcg cgcgcagcgt attctgcgag aaatcaatca acatcatcgg
    11941 cataaattcg cccatttcga tgagaatttc gttgaacgtc tgctaaatca tccggaagtg
    12001 ccagaactaa tccgcaccag cccgttgacc caacgtgaat ggcaggtcct ggggctgatc
    12061 tactccggtt acagcaatga acaaattgcg ggcgaactgg aagtggcggc aaccaccatc
    12121 aagacgcata tccgcaatct gtaccaaaaa ctcggcgtcg cccaccgtca ggctgccgtg
    12181 cagcacgcgc agaaactgct gaagatgatg ggttatgggg tgtgagttta gccggataat
    12241 gcattggatc cggtttacat ctttacatca ttcaatactc acccgcgtta cgccatctgt
    12301 ttctatcaaa ccaaaccgca ccggcaagaa acgctccacc accgcgatat tggtcagcag
    12361 atggcatgaa ggatgggcga ccgtaaattc tcccgcgccc gccagcgcca tcggcagtac
    12421 cagttggtcg gcgagatatt cccccaccgc cgctgggctt gccaggtagc gtttcacctc
    12481 tttcactaac tgcgcggcta ccacctcggc actgacgcgc ttttcaccga cgacgaaaaa
    12541 gcgttcagtg atattttcac tttcgacttc cagcgagacg gtattacccg gcccctggtc
    12601 gcgcggcagg ttatgaatat tctgttcatg cagggaaaaa atccccgcca gtgtagcgat
    12661 ttcacgctca gcaacatggc gcggcacacc agctaataga acttctccac gcatctgcac
    12721 aatgttcccg cgctcgccaa gttgcaaggt gctaaacgat gccacgggcg agacttccgt
    12781 tgccaccaca ccgcctccgg caggataaaa accgtgacgt aacagcgtgg tttgctgatg
    12841 aattcctatt ttcgccagca gcggctccag cacccggcgg ataaaatccg caggcggcgc
    12901 cgacgggtta tcggtgccgc cgctcacttc aacacgcgaa ggtccatcgg caaaccacag
    12961 cgcgggcagc accgtttgca gcaccagcgt acaacttccg gcgctaccga tagcaaagcg
    13021 gtaatcgccg ccgcgcacgg tgccaggccg gaagaccaga cgctgcgatc ccagctccgc
    13081 gccttccacc gttgccctac aaatttccgc agccgctttt accgcggtca gatgctggcg
    13141 cagcagcccc ggtttcgccc gtccggctcg aatgccagtg atggtaaatg gctggccggt
    13201 tatcatcgac aggctcagcg ccgagcgcag gatctgcccg ccgccttcgc cctgtgcgcc
    13261 atccagcgca atcatccttt tcatcattta tccttttacg cacaccacct gacgcagggt
    13321 gtagataact tccaccagat cgctttgtgc cgccatcacc gcatcaatat ctttatacgc
    13381 catcgggatt tcgtcgatca cttcggcatc tttacggcat tccacatgcg cggtggcacg
    13441 aatttggtct tccacgctga acagtttttt cgctttagtt cggctcatta cccgcccggc
    13501 accgtggctg cacgaacaga acgactcttc atttcccagc ccacggacga taaagctttt
    13561 tgctcccatc gaaccgggaa taattccata ttgaccagca cgcgcagaca ccgcaccttt
    13621 acgcgtcacg tagatctctt caccaaagag ctgttctttt tgcacatagt tgtggtgaca
    13681 gttgatctct tccatcgcca gcgtttgtgg ctgtctgacc gttttctgcg taatgctctg
    13741 caacgccgtt accacatttt ccatcatcgc atcgcggtta aggctggcga aaagctgcgc
    13801 ccaggccacg gctcttaggt aatcatcaaa gtattccgta ccttccataa agtacgccag
    13861 atcacgcgac ggcaacgtct caagttggtc ctgcatctct ttttgcgcca gatcgataaa
    13921 gtaagtcccg atggcgttac caattccgcg tgaaccggag tgcagcataa tccacacctg
    13981 ctccgactca tcaaggcaga tttcaataaa gtggttaccc gttcccagcg ttcccaggtg
    14041 tttatagttg ttagtgttaa ggaaacgcgg atatttttgc gttaaccact gataaccggc
    14101 ctccagctca gcccatttcg catcgacgtt aaaaggcggg ttttcccatg cgcctttatc
    14161 acgtttacaa cgcccggtag tacgcccgtg tggtacggcc gtttcaatcg cttgacgcag
    14221 ctccgccagg ttttcaggca ggtctgccgc cgttaacgcg gtacgcagcg cgttcattcc
    14281 acagccaata tccacgccca ccgccgccgg aataatcgcc cctttggtcg ggatcacgct
    14341 accaatggtg gaacctttac ccaggtgtac atcaggcatt accgcaatat gtttgaaaat
    14401 aaacggcatc ttcgccgtat taataagttg ctgacgcgca tcggcttcta ccggcacgcc
    14461 tttggtccac atttttaccg gggcattttc agtggtcagt aattcgtaat tcattttgtt
    14521 tttctctttt cgttgtttgc ggtcctgata attgcaaccg tcgtgccaga aaattaaata
    14581 agcagattaa ttttttaatt tattgtttct aaaaagatta tatctttatg tccgtaaacg
    14641 gagatttccc gcaaagccga tttaccgata atgaaatatc gccttttata aggatatcta
    14701 agatgcgtaa aacagtggct tttggctttg tcggtaccgt gctggattat gccggacgcg
    14761 gcagtcagcg ctggtcaaaa tggcgtccgt cactctgtat ataccagcaa gaatcgttgg
    14821 tcatcgatcg actggaatta ttgcacgatg cccgctcgcg ctcgctattt gaaacgctta
    14881 aacgcgatat cgccagcgtt tcgccagaaa cagaagtggt gggcgttgag attgaactgc
    14941 ataacccgtg ggatttcgaa gaggtttacg cctgcctgca tgatttcgcc cgtggttacg
    15001 agtttcagcc agaaaaagaa gactatttaa ttcacatcac caccggcacc cacgtcgcgc
    15061 agatttgctg gtttctgctg gcggaagcgc gttatctacc cgcccggttg atacaatctt
    15121 caactccgcg caaaaaagaa cagccgcgcg gcccaggtga agtgacgatt atcgatctcg
    15181 atttaagccg ttataacgcc atcgccagcc gctttgccga ggaacggcag caaacgctcg
    15241 atttcctcaa atccggtatt gccacgcgta acccccactt caaccgcatg attgagcaga
    15301 tcgaaaaagt ggcgattaaa tcccgcgcgc cgattctgct taacggcccg accggcgcag
    15361 gtaagtcatt tctggcgcga cgcatcttcg agttaaaaca ggcacggcat cagtttagcg
    15421 gcgcgtttgt ggaagtgaac tgcgccaccc tgcgcggcga taccgcgatg tcgacgctgt
    15481 ttggtcatgt gaaaggcgcg tttaccgggg cgcgggaatc tcgtgaagga ttattacgca
    15541 gcgccaacgg cggaatgttg tttcttgatg agattggcga actgggcgca gacgaacagg
    15601 caatgctgct gaaagctatt gaagagaaaa ccttttaccc gtttggcagc gatcgccagg
    15661 tgagcagtga ttttcagctt atcgccggaa cggtgcgcga tttgcgccag ctggttgccg
    15721 aaggcaaatt ccgcgaagat ctgtacgcgc ggatcaatct ctggaccttc accctgccgg
    15781 gtctacgcca gcgccaggaa gatattgaac cgaacctgga ttatgaagtg gagcgccacg
    15841 ccacactcac tggtgacagc gtgcgtttta acaccgaagc gcggcgtgcc tggctggcct
    15901 ttgcaacatc tccccaggca acatggcgcg gtaactttcg cgaactttct gccagcgtca
    15961 cgcggatggc cacctttgcc actagcggac gcatcactct ggacgtggtg gaagatgaga
    16021 taaaccgtct gcgctataac tggcaggaga gccgcccctc cgcgcttacg gcgttgctgg
    16081 gcgcagaggc agaaaacatc gatctcttcg accgtatgca actggaacac gttatcgcta
    16141 tctgccgcca ggcaaagtcg ctttctgctg ccggacgaca gctttttgat gtttcgcgcc
    16201 agggcaaagc cagcgtcaat gacgcggatc ggctacgcaa atacctggcg cgttttggtc
    16261 tgacgtggga agccgtgcag gatcagcaca gctccagttg aatatggtgg tccgtcagca
    16321 cctgcatcac gcttactggc ggcggggcgt cggtgtagac ggcatcaacc atgctgatgc
    16381 tccccatatt gaccatcgca ttacggccaa attttgaatg gtcaaccacc agcataacgt
    16441 ggcgcgagtt ctcaataatg gcgcgcttgg tacgaacttc gtggtaatcg aactccagca
    16501 gcgagccgtc gctgtcgatg ccgctgatcc ccagaatgcc gaaatcaagg cggaactggg
    16561 agataaaatc gagcgtcgct tcgccaatga tcccgccatc gcggctacgt aattcgccac
    16621 cggcgagaat aatgcggaaa tcttctttta ccatcaacgt gttagcaacg ttgagattgt
    16681 tggtgacaat gcgcaaattg ctgtgattga gcagcgcgtg cgccaccgct tccggcgtgg
    16741 tgccgatatc gataaacagc gtcgagccat tggggatttg ctccgccact ttgcgggcga
    16801 tgcgctcttt ttcttcggtc tgggtggctt tacgatcgtg ccacggcgtg ttaaccgaac
    16861 tggaaggcag cgccgcgccg ccatgatggc gcaggatcag gttttgctct gccagctcgt
    16921 tgaggtcgcg gcgaatagtc tgcgggctga cggagaagtg ctccaccagt tcctcggtac
    16981 tgacataacc ctgctgttta accagttcga taataccgtt gtgacgttgt gtttgtttca
    17041 tttataaatc cctggaatta ttttcgtttt cgcgcattga gcgaatcaac aaaagccatc
    17101 gctaaaccca cggctaatcc ggcgatgtgt gctccgttcg ccatcgacat cccaaacaaa
    17161 tcaaaccatc cggcgacaat ccagatcagc gcaaagataa ttaacccacg ttgcaggtaa
    17221 atgccacttt gcggatcgcg ttcgccacgt agccagacgt agcccatcag cgcatacacc
    17281 acgccagaaa gcccgccaaa ccacggcccg ctgaattttt gctgcatata gccgcttaac
    17341 agggcgctga taagcgtaat gacaattagc ttaccgctac cgaggcgttt ttccaccgcg
    17401 ccgccgagat accaccacca aagcaggtta aagaggatat gcatcagcga gaagtgcatt
    17461 aacgcgtggg tgaagtaacg ccagaactca aatttcagtg ttggatcgaa tggccaggcc
    17521 agccataaca tcacttcctg atcgccgaga atttgcatgg caataaacac caccacgcag
    17581 gcgatcatca tcacccaggt caccggacct gcgcgttcac gcaaggcagc aaagaaagga
    17641 taacggcgat aatgcaggcc actgccggta tggcctgcca gccagctggc cgccagataa
    17701 cgcggatctg ccgggttttc gagaaaacgc gccagctccg cccgcacgcg ctcggcctgg
    17761 gactcatccg ccagccagac atcgctttgg ttatgttgtt gaatcgtgag gataacaccc
    17821 tgcgtcgcca tgtaatcaac aaacgcctgc gccacgcggg ggttagcaaa agaggtaatc
    17881 atcaacatcg ttgctgtcgc ttattccaca caaaagggga cagtataaag cgttacgcgc
    17941 cgtacgccac ctctgcggga aactgtcgct gccaggcttc aaagccgccg tcaatgctat
    18001 agaccacatc gtagccctgt tgcagcagat actgcgccgc ccctttgctg ctattgccgt
    18061 gataacacat caccatcacc ggagtgtcaa agtcgttatc acgcataaac gcgcccagcg
    18121 tgtcgttggt taaatggaaa gcctgcaccg catgccccat agcgaaactc tgtggatcgc
    18181 gaatatcgac cagcaccgcc tctttttcct gcaacttctg gtgcgcgtcg gcaacgttaa
    18241 tacattcgaa ctgatccatg cgtctctctt tctttacaaa caagtgggca aatttaccgc
    18301 acagtttacg tcgaagcggc agataaacgc cataatgtta tacatatcac tctaaaatgt
    18361 tttttcaatg ttacccaaag cgcgattctt tgctaatatg ttcgataacg aacatttatg
    18421 agctttaacg aaagtgaatg agggcagcat ggaaaccaaa gatctgattg tgataggtgg
    18481 cggcatcaat ggtgctggta tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat
    18541 gctggaggcg caggatctcg cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg
    18601 tggcctgcgc taccttgagc actatgaatt ccgcctagtc agcgaggcgc tggctgaacg
    18661 tgaagtgctg ctgaaaatgg ctccgcatat cgccttcccg atgcgttttc gcctgccaca
    18721 tcgtccgcat ctgcgcccgg cgtggatgat tcgcattggt ctgtttatgt acgatcatct
    18781 gggtaaacgc accagcctgc cgggatcaac tggtttgcgt tttggcgcaa attcagtgtt
    18841 aaaaccggaa attaagcgcg gattcgaata ttctgactgt tgggtagacg acgcccgtct
    18901 ggtactcgcc aacgcccaga tggtggtgcg taaaggcggc gaagtgctta ctcggactcg
    18961 cgccacctct gctcgccgcg aaaacggcct gtggattgtg gaagcggaag atatcgatac
    19021 cggcaaaaaa tatagctggc aagcgcgcgg cctggttaac gccaccggcc cgtgggtgaa
    19081 acagttcttc gacgagggga tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg
    19141 cagccatatt gtggtgccgc gcgtgcatac ccaaaagcag gcctacattc tgcaaaacga
    19201 agataaacgc attgtgttcg tgatcccgtg gatggacgag ttttccatca tcggcaccac
    19261 cgatgtggaa tacaaaggtg atccgaaagc agtgaagatt gaagagagtg aaatcaatta
    19321 cctgctgaaa gtgtataacg cgcactttaa aaagcagcta agccgtgacg atatcgtctg
    19381 gacctactcc ggtgtgcgtc cgctgtgtga tgatgagtcc gactcgccgc aagccattac
    19441 ccgtgattac acccttgata tccatgatga aaacggcaaa gcaccgctgc tgtcggtatt
    19501 cggcggtaag ctgaccacct accgaaaact ggcagaacat gcgctggaaa aactgacgcc
    19561 gtattatcag ggtattggcc cggcgtggac gaaagagagc gtgctgccgg gtggtgccat
    19621 tgaaggcgac cgcgacgatt acgccgctcg cctgcgccgc cgttacccgt tcctgactga
    19681 atcgctggca cgtcactaca cgcgcactta cggcagcaac agcgagctgc tgctcggcaa
    19741 tgcgggaacg gtaagcgatc tcggggaaga tttcggtcat gagttctacg aagcggaact
    19801 gaaatacctg gtggatcacg aatgggtccg ccgcaccgac gatgccctgt ggcgtcgcac
    19861 aaaacaaggc atgtggctga atgcggatca acaatctcgt gtgagtcagt ggctggtgga
    19921 atatacgcag cagaagttgt cgctggcgtc gtaaattaac gtaaggtgat caggtcagat
    19981 ttcaatctgg cctgagactg atgacaaaca caaaactgcc tgatgcgcta cgcttatcag
    20041 gcctacgtgg tttatgcaat atattgaatt tgcatggtct tgtaggccag ataagacgtt
    20101 cacgtcgcat ccggcatgaa caaagcgcac tttgtcaaaa atctaaccta ctttttaatt
    20161 cagggaatta ccgcaaagcc cacgccatca tacccaacgt aacaaaactc aggcacgttc
    20221 cccactcgcc ccgagaaaaa tagcattaat gcgcccagcg tcagcataaa aatattgagc
    20281 agaaggtaaa ttgttggcgt aaagagtaaa ttgcaaacct caggaatagc ggcaagccca
    20341 gcccccagca tggcaataac cgctgcaa
//
LOCUS       DAJVPP010000073        20332 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVPP010000073 DAJVPP010000000
VERSION     DAJVPP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.073"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..4166)
                     /gene="mukB"
                     /locus_tag="OQM34_002692"
     CDS             complement(<1..4166)
                     /gene="mukB"
                     /locus_tag="OQM34_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1453075.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDES"
     gene            complement(4166..4870)
                     /gene="mukE"
                     /locus_tag="OQM34_002693"
     CDS             complement(4166..4870)
                     /gene="mukE"
                     /locus_tag="OQM34_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1453076.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4851..6173)
                     /gene="mukF"
                     /locus_tag="OQM34_002694"
     CDS             complement(4851..6173)
                     /gene="mukF"
                     /locus_tag="OQM34_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1453077.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6170..6955)
                     /gene="cmoM"
                     /locus_tag="OQM34_002695"
     CDS             complement(6170..6955)
                     /gene="cmoM"
                     /locus_tag="OQM34_002695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1453078.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7091..7870
                     /gene="elyC"
                     /locus_tag="OQM34_002696"
     CDS             7091..7870
                     /gene="elyC"
                     /locus_tag="OQM34_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1453079.1"
                     /translation="MLFTLKKVIGNMLLLLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7847..8740)
                     /locus_tag="OQM34_002697"
     CDS             complement(7847..8740)
                     /locus_tag="OQM34_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1453080.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8894..9640)
                     /gene="kdsB"
                     /locus_tag="OQM34_002698"
     CDS             complement(8894..9640)
                     /gene="kdsB"
                     /locus_tag="OQM34_002698"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1453081.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9637..9819)
                     /gene="ycaR"
                     /locus_tag="OQM34_002699"
     CDS             complement(9637..9819)
                     /gene="ycaR"
                     /locus_tag="OQM34_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1453082.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9871..11103)
                     /locus_tag="OQM34_002700"
     CDS             complement(9871..11103)
                     /locus_tag="OQM34_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1453083.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11140..12126)
                     /gene="lpxK"
                     /locus_tag="OQM34_002701"
     CDS             complement(11140..12126)
                     /gene="lpxK"
                     /locus_tag="OQM34_002701"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1453084.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12123..13871)
                     /gene="msbA"
                     /locus_tag="OQM34_002702"
     CDS             complement(12123..13871)
                     /gene="msbA"
                     /locus_tag="OQM34_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1453085.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(13908..16167)
                     /locus_tag="OQM34_002703"
                     /pseudo
     CDS             complement(13908..16167)
                     /locus_tag="OQM34_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16374..16658)
                     /gene="ihfB"
                     /locus_tag="OQM34_002704"
     CDS             complement(16374..16658)
                     /gene="ihfB"
                     /locus_tag="OQM34_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1453086.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16818..18491)
                     /gene="rpsA"
                     /locus_tag="OQM34_002705"
     CDS             complement(16818..18491)
                     /gene="rpsA"
                     /locus_tag="OQM34_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1453087.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18602..19285)
                     /gene="cmk"
                     /locus_tag="OQM34_002706"
     CDS             complement(18602..19285)
                     /gene="cmk"
                     /locus_tag="OQM34_002706"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1453088.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19458..20222)
                     /gene="ycaL"
                     /locus_tag="OQM34_002707"
     CDS             complement(19458..20222)
                     /gene="ycaL"
                     /locus_tag="OQM34_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS1453089.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4892 a         5638 c         4927 g         4875 t
ORIGIN      
        1 gattcatctt cccagctttg taccaccatc accagaatcg acataccggt accaatcgcc
       61 tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc acggttaacc
      121 tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat ggtctgcggc
      181 gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc caacgcttcc
      241 gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg ttcagagagc
      301 acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac gctgttcacc
      361 tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt ctgctcgcgc
      421 tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag tttctgttca
      481 cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc gatagcttcc
      541 accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg ctgataaacc
      601 gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat gcgcagcacg
      661 tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc cttatccgac
      721 atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa gcggcgctca
      781 acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc ggtcactacc
      841 tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt caggttgtcc
      901 atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga acggttattg
      961 ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc cccgctatca
     1021 gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc ttttttggtg
     1081 tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg cgctgcgtga
     1141 ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag acgttcacgc
     1201 agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga atagctaaag
     1261 tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc atcgcgctgc
     1321 atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg gtcgctctgc
     1381 aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg aacaaaacgc
     1441 gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc cgccaggctg
     1501 tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac gccttctttc
     1561 gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact caacgcccgc
     1621 tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc agactcaaac
     1681 gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg ctgggttttc
     1741 tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc ggcatggagg
     1801 ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg gaaacgtgaa
     1861 taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc atcaacgctg
     1921 aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc ttccgggcaa
     1981 tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac gatggcgtgg
     2041 cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac gtcgtcataa
     2101 atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg ctgatcttca
     2161 gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt gcgcgcgcct
     2221 acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg cagatattca
     2281 gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg gttgagactg
     2341 ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg agactgcagc
     2401 tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac gctatcggaa
     2461 agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc aatatcaaaa
     2521 ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg ctgctcacgc
     2581 agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc tgccaggtga
     2641 cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc gttacgcgcc
     2701 agcgggccgt tgattgccac caccagctga taagcctgct caaactggct gtgcgcggtt
     2761 tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc cagctctttc
     2821 gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg cagatggcac
     2881 agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc gcgcgtttgc
     2941 tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc cacttccagt
     3001 tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc tgccaccact
     3061 tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta gcgttcgatc
     3121 ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc cgcctgataa
     3181 tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc catatcgacg
     3241 tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg acgaaactcc
     3301 agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc cgccgccacg
     3361 tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg ggtgacacga
     3421 atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg gaacgcttta
     3481 cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat ggcactggag
     3541 atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc agatgcggag
     3601 cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc ggtaatggag
     3661 ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag ctcgttaagc
     3721 ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt cggctgcacc
     3781 gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc gcgtccggca
     3841 acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat ggtgtcgagc
     3901 atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg cgaaccgctg
     3961 gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg gatcagcgcc
     4021 gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga aagcgtcgtg
     4081 accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat cagcgtcagt
     4141 gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc tgactctctt
     4201 cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca tcacgaatca
     4261 ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg aagcggaaca
     4321 ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc atgcctaaac
     4381 gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg tcaacgtctg
     4441 aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc agggtgagca
     4501 gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt tccgggctga
     4561 gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag acggaacgcg
     4621 ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg cgaataagct
     4681 caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc aggaatgcat
     4741 gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg tccagcgccg
     4801 gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt tcaatatttg
     4861 tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca tttcgctggc
     4921 agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat aacaatacgc
     4981 gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac caccagacca
     5041 agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat gatcgccgcc
     5101 agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag ttccccagtc
     5161 acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg atcggcattg
     5221 gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg taaccgctga
     5281 gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg tacgtggcgg
     5341 tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg atcgagtttg
     5401 ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt catcgtcgca
     5461 tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag cgtatcctgc
     5521 aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga aatcgccgcc
     5581 cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg ctgttcgtcc
     5641 atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata tttcagtggc
     5701 gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc atctgctgcg
     5761 cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt agaaaactcg
     5821 cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg gtaaattgcg
     5881 ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac catgtcgttg
     5941 atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc atcactcaca
     6001 tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg ctcgccgttc
     6061 agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat ggagaagtca
     6121 ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact catactttat
     6181 ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta atatacggtt
     6241 cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg cgctgctggt
     6301 acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata atttgccaac
     6361 cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct ggcgaaagcg
     6421 tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg gcgaccatgt
     6481 tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg cctggacgca
     6541 acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg agcaccgcat
     6601 ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga gcggcgcaat
     6661 gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt gcgcggtcga
     6721 tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc attttgattg
     6781 cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg cccatttccg
     6841 ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct ttggtggtgc
     6901 cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc tgcattgctt
     6961 tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac agccacggcg
     7021 agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt taaaaaagga
     7081 accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc tgctacttcc
     7141 gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc gttttcaaaa
     7201 aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga gtttgcaacc
     7261 ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga ataattcgca
     7321 aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc agtgggcacc
     7381 gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc gtttatggcg
     7441 cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca atacggtgag
     7501 tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg agcaaattat
     7561 caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac aggcgattgg
     7621 tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga tgatcttttt
     7681 tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca tcgactcgcc
     7741 gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata gcgatcgtgt
     7801 tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag gcgagccacg
     7861 gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg gtattaacca
     7921 gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac gactccgcca
     7981 ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta tccattagtc
     8041 ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc atcgccagta
     8101 actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg tgaatcaaca
     8161 cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg cgaaacagga
     8221 tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg gtccatagca
     8281 cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg tcgaccgcgc
     8341 cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg atttggtctt
     8401 tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca ccacgcatcc
     8461 gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca ccataaatag
     8521 tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc agttgtcggg
     8581 cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc tggctgtcat
     8641 acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga ctgagttttt
     8701 cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc ctcataaaag
     8761 ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga tcaattgttg
     8821 aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt ggctgaagtg
     8881 tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca ggggtatcca
     8941 cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg taccacagaa
     9001 cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag ttgacgtaac
     9061 gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag ttatcgccaa
     9121 cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca cgtgagaagt
     9181 acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta aacgcttctt
     9241 ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga gcgaggttat
     9301 cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc acattaacga
     9361 tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt tctgttcctg
     9421 actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg gcgcgggcaa
     9481 catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc gcgcgttcaa
     9541 gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg ggcagacgcg
     9601 tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct catcagcagt
     9661 cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg ggaaagccag
     9721 gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt ttccgttgca
     9781 aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag atggatcggg
     9841 ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt cccagccagt
     9901 gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg ttgcctgctg
     9961 ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg tcgttggttt
    10021 aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc gatgcatttt
    10081 ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa aatagccata
    10141 ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa aaagctgctc
    10201 tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc tgtgcgttgc
    10261 agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat gcagccaaag
    10321 attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc tcgcttcgcg
    10381 ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact gttcgcggaa
    10441 tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg tttgcgaaac
    10501 gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat aaacgcgctg
    10561 gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt ccagatgccg
    10621 tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct caaaatcggc
    10681 tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt ccgcctcatg
    10741 ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca gcatgcggtg
    10801 gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc agtattccat
    10861 taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc ccagacgact
    10921 gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt gcagcaagga
    10981 catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg gtttgtttaa
    11041 caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt gcggcagcga
    11101 cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag aagccagcga
    11161 ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt ctacaggcaa
    11221 ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt cagtcattac
    11281 cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag actgatgatc
    11341 ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg caaaaaagcg
    11401 cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa cgtcacaacg
    11461 cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga tttcaccgct
    11521 gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc ccgctcgctc
    11581 acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac catcaataac
    11641 gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg ttacgatgat
    11701 ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa cgggagaaac
    11761 cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg cctgtgctgt
    11821 ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat atccccgcga
    11881 tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga caacgaccgg
    11941 ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta cgggggcacg
    12001 ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca ccaggccata
    12061 caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc agattttttc
    12121 gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg ctcaagcaaa
    12181 tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat ttcgtctgcc
    12241 ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt ctgcaactca
    12301 tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt agcttcatcc
    12361 agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg ctgacgctga
    12421 ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt atccatctta
    12481 ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc acggctgtac
    12541 tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag atggacattc
    12601 tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg cagatcgtga
    12661 ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag gctggcgatg
    12721 gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc cggaattttc
    12781 aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt gacattgcgg
    12841 aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt ctcctgctca
    12901 ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa ctgggcgtta
    12961 acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa aacaacggta
    13021 atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata cagaacaaac
    13081 gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga ggctgaaacc
    13141 attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt cgtttccact
    13201 tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc tgcactggtg
    13261 gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg cttcgatact
    13321 acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga cagttgccaa
    13381 ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg cacaacagta
    13441 atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat acgtgacaac
    13501 agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat gtgaccaaac
    13561 aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca gtagctggag
    13621 acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg catccacacc
    13681 agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag gagcgataac
    13741 atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat cagacccgct
    13801 ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga gagatctttg
    13861 tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta cccgttatcc
    13921 actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct gaggctgcta
    13981 atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg agtatcaagc
    14041 cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa tcgccatgcg
    14101 ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat taatggcaac
    14161 gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg cacttgctgc
    14221 cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt tagaagaatg
    14281 ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt atcgttgcta
    14341 ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc ttcgccacgc
    14401 acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat cggccatata
    14461 tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag aataacgcct
    14521 tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc actatcccct
    14581 tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca ccattgccag
    14641 cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt tttgccacag
    14701 cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc gccaggcgtt
    14761 taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc aacattcaac
    14821 aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag ccaaagaccg
    14881 gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat gcacaaccat
    14941 cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa atagatttgc
    15001 aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca taagcaggag
    15061 tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg gcggcagttg
    15121 ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg cagcggcaga
    15181 gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg ccgccagaca
    15241 acatatccat acatcccagc cactccactg tcgcccactt aacttaagca ttccccacgt
    15301 agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa
    15361 agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc gcccaggcag
    15421 aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa
    15481 tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt tgatttcttt
    15541 ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc acggataggg
    15601 ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat tcggttcaat
    15661 gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg cataacgctg
    15721 gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga gtttcattga
    15781 ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca caaggcctgg
    15841 cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag aggtcatgcc
    15901 gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc
    15961 ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat
    16021 ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag tcagaaacgc
    16081 aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc cgcaaattat
    16141 gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa gtgtgcaact
    16201 ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca gaagaattta
    16261 acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct gattagagaa
    16321 ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg
    16381 taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac gtattttcct
    16441 tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc
    16501 aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag agtcgaggcc
    16561 atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat gtgcgattgc
    16621 tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt tccttaaagg
    16681 caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc ccttagtgct
    16741 tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg gaataaaaat
    16801 cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc cattgcgttg
    16861 ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct
    16921 tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac gccggtgaat
    16981 ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac gcggtcacgg
    17041 gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac ggttgcgcct
    17101 ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt cagagcaacc
    17161 cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg ttcacgttct
    17221 gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct
    17281 tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg
    17341 tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc aacacggtcg
    17401 cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg tttcagaccc
    17461 agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac tacatcgcca
    17521 acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt
    17581 accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt caggttggtc
    17641 acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac ccacggatct
    17701 tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa cttcagcact
    17761 ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac gcgtttccag
    17821 gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa tgcaccgtag
    17881 tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt ttccagcagc
    17941 tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac aacaacgttg
    18001 ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc caggtgcaga
    18061 gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg aataccgttc
    18121 agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac agtttcagca
    18181 tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt ctcacgggac
    18241 agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac ttcgtcacct
    18301 acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat ggcggactca
    18361 gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac aacgccacga
    18421 acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag ttgagcaaaa
    18481 gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc catgccggat
    18541 ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca aattcggtcg
    18601 cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc aattgctcaa
    18661 tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt ggcgctaccg
    18721 ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc tcgaagttaa
    18781 cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct tccgaggagg
    18841 cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg tcgcggccat
    18901 cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa
    18961 cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta cgaatttcgc
    19021 cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac acaaaacgta
    19081 catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca acatggtgat
    19141 gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga tgccattgca
    19201 acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg ccatcaatgg
    19261 taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac cgttaacata
    19321 accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt
    19381 ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag aagaccagcc
    19441 cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg ttgcgcacgc
    19501 tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc caggctagcc
    19561 agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt caacagatca
    19621 aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt aatagcgcct
    19681 tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct ggtagctgaa
    19741 atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag cgagtgaccc
    19801 aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc attcatcatg
    19861 tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc gttgacgtcg
    19921 ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc caatgctttg
    19981 gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg attgccgctg
    20041 tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct taatgttgct
    20101 gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc gtgggtattt
    20161 tgacaccctg tcagtaatgc tgcagaggcc gcaatcgcca gcagtaattt agtattcttc
    20221 atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac ttacattgac
    20281 aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg cc
//
LOCUS       DAJVPP010000074        20280 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVPP010000074 DAJVPP010000000
VERSION     DAJVPP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.074"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..63
                     /locus_tag="OQM34_002708"
     CDS             <1..63
                     /locus_tag="OQM34_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1453090.1"
                     /translation="SIKNEAVNAIIATLKNVYCP"
     gene            73..597
                     /gene="ygaP"
                     /locus_tag="OQM34_002709"
     CDS             73..597
                     /gene="ygaP"
                     /locus_tag="OQM34_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1453091.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(644..1048)
                     /gene="stpA"
                     /locus_tag="OQM34_002710"
     CDS             complement(644..1048)
                     /gene="stpA"
                     /locus_tag="OQM34_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1453092.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGLNPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            1716..2165
                     /gene="alaE"
                     /locus_tag="OQM34_002711"
     CDS             1716..2165
                     /gene="alaE"
                     /locus_tag="OQM34_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1453093.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2202..2546)
                     /locus_tag="OQM34_002712"
     CDS             complement(2202..2546)
                     /locus_tag="OQM34_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS1453094.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            2698..2928
                     /locus_tag="OQM34_002713"
     CDS             2698..2928
                     /locus_tag="OQM34_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453095.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3275..3520
                     /gene="nrdH"
                     /locus_tag="OQM34_002714"
     CDS             3275..3520
                     /gene="nrdH"
                     /locus_tag="OQM34_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1453096.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3517..3927
                     /gene="nrdI"
                     /locus_tag="OQM34_002715"
     CDS             3517..3927
                     /gene="nrdI"
                     /locus_tag="OQM34_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCS1453097.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            3900..6044
                     /gene="nrdE"
                     /locus_tag="OQM34_002716"
     CDS             3900..6044
                     /gene="nrdE"
                     /locus_tag="OQM34_002716"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1453098.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6054..7013
                     /gene="nrdF"
                     /locus_tag="OQM34_002717"
     CDS             6054..7013
                     /gene="nrdF"
                     /locus_tag="OQM34_002717"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1453099.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPVEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7368..8570
                     /gene="proV"
                     /locus_tag="OQM34_002718"
     CDS             7368..8570
                     /gene="proV"
                     /locus_tag="OQM34_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1453100.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALYALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8563..9627
                     /gene="proW"
                     /locus_tag="OQM34_002719"
     CDS             8563..9627
                     /gene="proW"
                     /locus_tag="OQM34_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1453101.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9685..10677
                     /gene="proX"
                     /locus_tag="OQM34_002720"
     CDS             9685..10677
                     /gene="proX"
                     /locus_tag="OQM34_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1453102.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            10869..12048
                     /locus_tag="OQM34_002721"
                     /pseudo
     CDS             10869..12048
                     /locus_tag="OQM34_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12172..12909
                     /gene="ygaZ"
                     /locus_tag="OQM34_002722"
     CDS             12172..12909
                     /gene="ygaZ"
                     /locus_tag="OQM34_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1453103.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            12899..13234
                     /gene="ygaH"
                     /locus_tag="OQM34_002723"
     CDS             12899..13234
                     /gene="ygaH"
                     /locus_tag="OQM34_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1453104.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13325..13855
                     /gene="emrR"
                     /locus_tag="OQM34_002724"
     CDS             13325..13855
                     /gene="emrR"
                     /locus_tag="OQM34_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1453105.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            13982..15154
                     /gene="emrA"
                     /locus_tag="OQM34_002725"
                     /pseudo
     CDS             13982..15154
                     /gene="emrA"
                     /locus_tag="OQM34_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15171..16709
                     /gene="emrB"
                     /locus_tag="OQM34_002726"
     CDS             15171..16709
                     /gene="emrB"
                     /locus_tag="OQM34_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1453106.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(16773..17288)
                     /gene="luxS"
                     /locus_tag="OQM34_002727"
     CDS             complement(16773..17288)
                     /gene="luxS"
                     /locus_tag="OQM34_002727"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1453107.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17438..18994)
                     /gene="gshA"
                     /locus_tag="OQM34_002728"
     CDS             complement(17438..18994)
                     /gene="gshA"
                     /locus_tag="OQM34_002728"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1453108.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19067..19495)
                     /locus_tag="OQM34_002729"
     CDS             complement(19067..19495)
                     /locus_tag="OQM34_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1453109.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19492..20058)
                     /gene="yqaB"
                     /locus_tag="OQM34_002730"
     CDS             complement(19492..20058)
                     /gene="yqaB"
                     /locus_tag="OQM34_002730"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS1453110.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4886 a         5190 c         5071 g         5133 t
ORIGIN      
        1 tccattaaaa atgaggcggt aaatgccatt atcgccaccc tgaaaaatgt ctattgtccg
       61 taaggagtca ctatggcttt gacaaccatt tcgccgcatg atgcacaaga attaatcgca
      121 cgcggcgcaa agttaatcga tattcgtgac gctgatgaat atcttcgtga acatattcct
      181 gaagcagatc tggctccatt atccgtgctg gaacagtcag gtcttccacc taaattacgt
      241 cgcgagcaaa ttatattcca ctgccaggca ggtaaacgca ccagtaataa cgccgataaa
      301 ttagcagcga ttgccgcccc cgcagaaata tttttactcg aagatgggat tgatggctgg
      361 aaaagagctg gattgccagt agcggtaaat aaatctcaac ccttgccgtt aatgcgccag
      421 gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg tactgggtta taccgtaaat
      481 agcggtttct tcttattaag tggctttgtt ggtgccgggt tactgtttgc aggaatcagc
      541 ggtttttgtg gaatggcaag gttgttagat aagatgccgt ggaaccaacg agcttgagaa
      601 gcgacgccgg acgcgcccta gcagcgacat ccggcctcag taattagatc aggaaatcgt
      661 cgagagattt accttctgcc agcgcctgag caattggctt cggtgtacgg ccctgaccgg
      721 tccaggtttt agtttcaccg ttaacatcag tgaatttata tttcgccgga cgcggctggc
      781 gttttttacc agcacgtggt gcagcagcag agctattacc caataactct tccgggttaa
      841 gtccgtcagc tttcatcagc tccagccagg tgctaatttt ttcctgacgc tcagccagtt
      901 cacgctgctg ctgttcttct tcttcacgtc tttctttagt gacaaccctg aatttttcga
      961 gcatttcttc aagaacgtca atggagaatt cgcgagccat cgcacggagg gtgcgaatgt
     1021 tatttaaact ttgtaacatt acggacataa taaagaaaac cttttaacgc caaaacaaaa
     1081 aagtatttcg tcgcgtagtg taagcgatca cttattatat ttccacaaaa tttaatctat
     1141 ttacagtcct gcgcgagatt atttcagctg ttattttctg aaactctctg aataatttca
     1201 agattatata ttagccattt tgatgagtaa tttaggttat tggtgaacag agtggcgctc
     1261 tctgctcgct aactccgcaa gcctgacaaa accgaagtga ataaaaatgc aatataacag
     1321 aataaaagaa aacacccctc actggtgctt tctgctgtta atgtacattt tctggcatca
     1381 aaaatgctgt aaatgacccg gtcacgacaa acaccgaatt ctctacgctt atagcgttac
     1441 ctccccccca aactttcatt agtgaaatta aaggcaagat catatctaat tgatttaatt
     1501 gaaaataaat catatcggca tgtaaaaatc cacaatgtac aaaaaaagag ccgttacgga
     1561 atattttatc tacaaaaact aactaaataa aaatttttca ctaattgatt agtcatagac
     1621 agcgatatac gctatgcaaa aatgcagatg acaatgagat ccactgcttt catctccatt
     1681 aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc
     1741 gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta
     1801 ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga ttggtagcga
     1861 ttccggtgaa catcttaatt gcatggccat acggtatgta ccgcgatctg tttatgcgcg
     1921 cggcacgcaa agttagcccg tcgggctgga taaaaaatct ggctgatatc ctggcttatg
     1981 tgacgttcca gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca gactggcatc
     2041 agattatggc ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg
     2101 gctacttcct cgattattgc cgccgactgt ttaaagtcag ccgttaccag caggtaaaag
     2161 cctgactctt cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat
     2221 aaaccattca aataacgttc aagcgcaaca cgcgaactaa agccatgcgg gataccgtaa
     2281 tgctcgtgtc gctcgcctgc taaatagagc ggaaattgct ggtaatgatc gcaagtttta
     2341 atatcggaag cgggttcagc cagtgttgaa ctgcgttctt ttaatgttgg atgaattacc
     2401 agagcggtac gtcccattcg cgcttcgcga ttaacataga cataattttc cccacggcgg
     2461 taaccatacg ctttctgggt taccacatcg acagtaaaac cgactttttc aagtacgcgc
     2521 gccacctcgt ctggtcgtaa atacatattt gatcctcgtt attttcaacc gcgggcctac
     2581 cttacctgat tgcgcattca caacgctttc agaaaagtcc ataaagccgc gatgaacctc
     2641 agttaagaaa attatggtct acactgaaaa ttacatcgaa ttctaatggg ggatcatatg
     2701 tttaaccgac cgaaccgcaa cgacgttgat gatggcgtgc aggatatcca gaatgatgtc
     2761 aatcaattag ctgacagcct cgaatctgta ttgaagtcct ggggcagcga cgccaaaggg
     2821 gaagctgaag ccgcacgcag taaagctcag gcgttgctga aagaaaccag ggcgcgaatg
     2881 catggtcgta ctcgcgtcca gcaagccgcg cgcgatgccg ttggctgagc ggattctttt
     2941 gttcgtgaaa gaccctggtg tagcgtgggt acagcagctg cggtaggtat ttttatcggc
     3001 gcactgttaa gcatgcgcaa atcgtagtgc aaaaatgata ataaatacgc gtctttgacc
     3061 ccgaagcctg tcttcggggt ttctttttgc ctggtgaatc acaaaaatcc ccctcccccg
     3121 tcacgctcat atccagggta atttcgatca ctatttgcta tatattgtgt ggttgaatct
     3181 tttttcaact acatctagta tctctgtatc aacagagaga caacccgacg cgtatcatcg
     3241 cgccgtatct tcattttaaa cggaaatacg aatcatgcgc ataactattt atactcgtaa
     3301 cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa aaccggggct ttgattttga
     3361 aatgattaat gtcgatcgcg ttcctgaagc agcggaagcg ttgcgtgctc agggctttcg
     3421 tcagttgccg gtagtgattg ctggcgatct tagctggtct ggtttccgtc cggacatgat
     3481 taaccgtctg catccagcgc cacacgcggc cagtgcatga gccagctcgt ctacttctcc
     3541 agcagctccg aaaacacgca gcgttttatc gaacgtttag gtctgcccgc ggtgcgcatc
     3601 ccgctcaatg agcgggaacg gattcaggta gacgagcctt acatcctgat cgtgccctct
     3661 tacggcggcg gcggtacggc tggcgcggtg ccacgacagg taattcgctt tttaaacgac
     3721 gagcacaacc gggcgttgct tcgcggcgtt attgcttctg gtaatcgcaa ctttggtgag
     3781 gcgtatggcc gcgccggaga tgtgattgcc cggaaatgcg gcgtgccgtg gctgtaccgt
     3841 tttgaactca tgggtacgca aagcgatatc gaaaacgttc gtaaaggagt aaccgaattt
     3901 tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat ggattaccac gcgctgaatg
     3961 cgatgcttaa tctttacgat agcgcaggtc gcattcagtt cgataaagac cgccaggccg
     4021 ttgacgcctt tattgcgacg catgtgcgtc cgaacagtgt gaccttcagt agccagcagc
     4081 agcgcctgaa ctggctggtc aacgaaggtt actatgatga aagcgttctt aatcgctact
     4141 ctcgcgactt tgtcattacg ctgtttgccc acgcacacac cagcggtttt cgtttccaga
     4201 cattcctcgg ggcatggaag ttttacacca gctatacgtt gaagacattc gacggtaaac
     4261 gttatctgga agattttgcc gatcgagtca cgatggtggc gctgacgctg gcacaaggcg
     4321 atgagacgct ggcgttgcaa ctgaccgatg aaatgctgtc aggacgcttt cagccagcca
     4381 cgccaacatt cctcaactgc ggtaagcagc agcgcggcga actggtttcc tgttttttgc
     4441 tgcgtattga agacaatatg gagtcgattg gtcgggcggt aaattccgca ctgcagctgt
     4501 cgaaacgcgg cggcggcgta gcatttttgc tgtcgaatct gcgggaagcg ggcgcgccaa
     4561 ttaaacgtat tgaaaatcaa tcttctggcg taattccggt gatgaaaatg ttggaagacg
     4621 cattttccta tgccaaccaa ctcggcgcgc gtcagggggc aggtgcagtc tatttacatg
     4681 ctcatcatcc cgatattctg cgttttctcg acacgaaacg ggaaaatgcc gacgaaaaaa
     4741 tccgcattaa aacactgtcg ctgggcgtgg tgatcccgga tatcactttc catctggcaa
     4801 aagagaatgc gcagatggcg ctgttttcgc cctatgacgt tgagcgagtt tatggcaagc
     4861 cgtttgccga tatcgccatt agcgaacact atgacgaact ggttgccgat gaacgcatcc
     4921 gcaaaaaata cctcaacgcc cgtgatttct tccagcgact ggcagaaatc cagtttgagt
     4981 ccggctatcc ctacatcatg tatgaagaca cagtaaaccg tgctaaccct atcgccgggc
     5041 gcataaatat gagtaatctc tgctcagaaa ttttgcaggt taacagcgcc tcagagtatg
     5101 acgagaatct cgactatgcc cgcacaggcc atgatatttc ctgcaattta ggttcgttga
     5161 atattgcgca caccatggat tcccccgatt tttcccgcac ggtagagact gccgtgcgcg
     5221 gtttaacggc ggtgtcagat atgagtcata tccgcagcgt gccgtccatc gaagccggaa
     5281 atgccgcctc gcacgccatc ggactggggc agatgaattt acatggttat ctggcgaggg
     5341 aaggcatcgc ttatggttcg ccggaagccc tggatttcac caatctctat ttctatacca
     5401 tcacctggca cgcactgcgt acctcgatgt tgctggcgcg cgaacgcggt gaaaccttcg
     5461 ccgggttcaa acagtcacgc tatgccagtg gtgaatattt cagccaatat ctgcaaggta
     5521 actggcagcc gaaaacggcg aaagttggcg aactgtttgc ccgcagctgt attacgttac
     5581 ctacccgtga gatgtgggcg caactgcgcg acgacgtgat gcgctacggc atatacaacc
     5641 agaatctcca ggcggtgccg ccaaccgggt ctatctctta tatcaaccat gctacgtcga
     5701 gtattcatcc gattgtggcg aaagtagaga tacgcaaaga gggcaaaaca ggacgcgttt
     5761 actaccctgc cccgtttatg actaacgaaa atctggcgct gtatcaggac gcttacgaaa
     5821 ttggcgcaga aaagatcatc gacacctacg cggaagcgac tcgccatgtc gatcaggggc
     5881 tgtcgctgac gctgtttttc cccgataccg ccaccactcg cgatatcaac aaagcgcaga
     5941 tttacgcctg gcgcaaaggt atcaaaacgc tctattacat ccgcctgcgt cagatggcgc
     6001 tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact ttaaggaata tctatgaaac
     6061 tctcacgtat cagcgccatc aactggaaca agatatctga cgataaagat ctggaggtgt
     6121 ggaatcgcct gaccagcaat ttctggctac cagaaaaggt gccgctgtcg aacgatattc
     6181 ccgcctggca gacattaact gtcgtagaac aacaactgac gatgcgcgtt tttactggcc
     6241 tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc ttcgctgatg cccgatgcgc
     6301 tcacgcctca tgaagaggcg gtattatcga atatcagctt tatggaagcg gttcatgccc
     6361 gctcttacag ttcgattttc tcgacgctgt gccagaccaa agatgtcgat gccgcctacg
     6421 cctggagtga agaaaacgca ccgttgcagc gaaaagctca gattattcag caacattatc
     6481 gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt tcttgaatct tttttgttct
     6541 attccggttt ctggctgccg atgtattttt ccagccgcgg aaagctgacc aataccgcgg
     6601 acctgatccg tctgattatc cgcgatgaag cagtccacgg ttactacata ggctataaat
     6661 atcagaaaaa catggaaaag atatctctgg gacaacgtga agagttgaag agtttcgcct
     6721 tcgatttgtt gctggaactc tacgacaacg agttgcaata caccgatgag ctgtacgccg
     6781 aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta caacaccaat aaggctttga
     6841 tgaatctggg ctacgaaccg ttatttcccg tagaaatggc ggaagtgaat ccggcaatcc
     6901 tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt cttttccggt tcaggctcct
     6961 cttatgtgat ggggaaagcg gttgaaacag aagatgaaga ctggaatttc tgagggtgtt
     7021 attttcaaaa atatcactac ccgcagcagg gaaataattc ccgccaaata gctttttatc
     7081 acgcaaatat tctgtagtga tctacactga tactctgttg cattattcgc ctgaaactac
     7141 aatattcagg cgttttttcg ctatctatga caaaaaatat caacttttct cgatttgctc
     7201 tcagccctta tatcacggaa aattccggcg atttgctcgc atcaatattc atgccacatt
     7261 tgccctcagg ggttgcctca gattctcagt atgttagggt agaaaaaagt gactattccc
     7321 attgggtaat atatcgacat aaacaaataa aggaatcatt ctattgcatg gccattaaat
     7381 taaaaattaa aaatctttat aaaatatttg gtgagcatcc acagcgagcg ttcaaatata
     7441 tcgaacaagg actttcaaaa gaacaaattc tggaaaaaac tgggctatcg cttggcgtaa
     7501 aagacgccag tctggccatt gaagaaggcg agatatttgt catcatggga ttatccggct
     7561 cgggtaaatc cacaatggta cgccttctca atcgcctgat tgaacccacc cgcgggcaag
     7621 tgctgattga tggtgtggat attgccaaaa tatccgacgc cgaactccgt gaggtgcgca
     7681 gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat gccgcatatg accgtgctgg
     7741 acaatactgc gttcggtatg gaattggccg gaattaatgc cgaagaacgc cgggaaaaag
     7801 ccctttatgc actgcgtcag gtcgggctgg aaaattatgc ccacagctac ccggatgaac
     7861 tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc gttagcgatt aatccggata
     7921 tattattaat ggacgaagcc ttctcggcgc tcgatccatt aattcgcacc gagatgcagg
     7981 atgagctggt aaaattacag gcgaaacatc agcgcaccat tgtctttatt tcccacgatc
     8041 ttgatgaagc catgcgtatt ggcgaccgaa ttgccattat gcaaaatggt gaagtggtac
     8101 aggtcggcac accggatgaa attctcaata atccggcgaa tgattatgtc cgtaccttct
     8161 tccgtggcgt tgatattagt caggtgttca gtgcgaaaga tattgcccgc cggacaccga
     8221 atggcttaat tcgtaaaact cctggcttcg gcccacgttc ggcactgaaa ttattgcagg
     8281 atgaagatcg cgaatatggc tacgttatcg aacgcggtaa taagtttgtc ggcgcagtct
     8341 ccatcgattc gcttaaaacc gcgttaacgc agcagcaagg tcttgatgcg gcgctgattg
     8401 atgcgccgtt agcagtcgat gcacaaacgc ctcttagcga gttgctctct catgtcggac
     8461 aggcaccctg tgcggtgccc gtggtcgacg aggaccaaca gtatgtcggc atcatttcga
     8521 aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa taatggctga tcaaaataat
     8581 ccgtgggata ccacgccagc ggcggacagt gccgcgcaat ccgcagacgc ctggggtaca
     8641 ccgacgactg caccggctga cggcggtggt gctgactggc tgaccagtac gcctgcgcca
     8701 aacgtcgagc attttaatat tctcgatccg ttccataaaa cgctgatccc gctcgacagt
     8761 tgggtcactg aagggatcga ctgggttgtt acccatttcc gtcccgtctt ccagggcgtg
     8821 cgcgttccgg ttgattatat cctcaacggt ttccagcaat tgctgctggg tatgccagcg
     8881 ccagtggcga ttatcgtttt cgctctcatc acctggcaga tttccggggt cggaatgggc
     8941 gtggcgacgc tggtctcgct gattgccatc ggcgcaatcg gtgcctggtc gcaggcaatg
     9001 gtgactctgg cgctggtgtt aactgccctg ctgttctgta tcgtcatcgg tttgccgttg
     9061 gggatatggc tggcgagaag tccgcgagcg gcgaaaatta ttcgtccact gcttgatgcc
     9121 atgcagacca cgccagcgtt tgtttatctg gtgccaatcg tcatgctatt tggtatcggt
     9181 aacgtgccgg gcgtggtggt gacgatcatc tttgcgctgc cgccgattat ccgtctgacc
     9241 attctgggga ttaaccaggt tccggcggat ctgattgaag cctcgcgctc attcggtgcc
     9301 agcccgcgcc agatgctgtt caaagttcag ttaccgctgg cgatgccgac cattatggcg
     9361 ggcgttaacc agacgctgat gctggccctt tctatggtgg tcattgcctc gatgattgcc
     9421 gtcggcgggc tgggtcagat ggtacttcgc ggtatcggtc gtctggatat ggggcttgcc
     9481 accgttggcg gcgtcgggat tgtgatcctc gccattatcc tcgatcgcct gacgcaggcc
     9541 gttgggcgcg actcacgcag tcgcggcaac cgtcgctggt acaccactgg ccctgttggt
     9601 ctgctgaccc gcccattcat taagtaactc tgcacttgcc cggtgacgcc gggcattatc
     9661 accctgccaa aaaaaggaat aacaatgcga catagcgtac tttttgcgac agcgtttgcc
     9721 acgcttatct ctacacaaac ttttgctgcc gatctgccgg gcaaaggcat tactgttaat
     9781 ccagttcaga gcaccatcac tgaagaaacc ttccagacgc tgctggtcag tcgtgcgctg
     9841 gagaaattag gttataccgt caacaaaccc agcgaagtgg attacaacgt tggctacacc
     9901 tcgcttgctt ccggcgatgc aaccttcacc gccgtgaact ggacgccact ccatgacaac
     9961 atgtacgaag ctgccggtgg cgataagaaa ttttatcgtg aaggggtatt tgttaacggc
    10021 gcagcacagg gttacctgat cgataagaaa accgccgacc aatacaaaat caccaacatc
    10081 gcacaactga aagatccgaa gatcgccaaa ctgttcgata ccaacggcga cggtaaagcg
    10141 gatttaaccg gttgtaaccc tggctggggc tgcgaaggtg cgatcaacca ccagcttgcc
    10201 gcgtatgaac tgaccaacac cgtgacgcat aatcagggga actacgcagc gatgatggcc
    10261 gacaccatca gtcgctacaa agagggcaaa ccggtgtttt attacacctg gacgccgtac
    10321 tgggtgagta atgagctgaa gccagggaaa gatgtggtct ggttgcaggt gccgttctcc
    10381 gcactgccgg gcgataaaaa tgccgatacc aaactgccga atggcgcgaa ttacggcttc
    10441 ccggtcagca ccatgcatat cgttgccaac aaagcctgga ccgagaaaaa tccggcggca
    10501 gccaaactgt ttgccattat gcagttgcca gtggccgata ttaacgccca gaacgccatt
    10561 atgcatgacg gcaaagcctc agaaggcgat attcagggcc acgttgatgg ctggatcaaa
    10621 gcccaccagc agcagttcga tggctgggtg aatgaggcgc tggcagcgca gaagtaattt
    10681 ttattcgggc ggataaggcg ttttcgcctc atccgccgct ctgtacacaa tgcctgatgc
    10741 gacgctgacg cgtcctatca tgcctacaaa ccacatcgca catttcaaca agctatcttt
    10801 catcccatat tcatcaacat ccgctattat tgatttccag cttaatcatc acctgatgaa
    10861 caaaaataat gactaaacct aatcatgagc ttagcccggc gctgatcgtg ctgatgtcta
    10921 tcgccaccgg tctggcggta gccagtaact attacgccca gccattgctc gacaccatcg
    10981 cgcgtaactt ttccctttcc gccagttcgg caggctttat tgttaccgcc gcgcagttgg
    11041 gctatgccgc aggtctactg tttcttgttc ccctcggtga tatgtttgaa cgccgccgcc
    11101 tgattgtctc gatgacctta ctggcagcgg gcggtatgtt aatcactgcc agcagtcagt
    11161 cactggcgat gatgatcctc ggtacggcat taaccggttt attctcagtc gtggcacaaa
    11221 ttctggttcc gctggcagcg acgctggctt caccggataa acgcggcaaa gtggttggca
    11281 ccattatgag cggtctgctg ttggggatat tgctggcacg aaccgttgcc gggttactgg
    11341 caaacctcgg cggctggcgc accgtctttt gggttgcttc ggtgttaatg gcactgatgg
    11401 cgctggcatt atggcgtggt ctgccacaaa tgaaatcaga aacccacctc aactacccac
    11461 agttgttggg ttccgttttc agcatgttca tcagcgataa gatcctgcgc acccgcgcgt
    11521 tgctgggctg catgaccttt gccaacttca gcattctctg gacctcaatg gcctttttgc
    11581 ttgccgctcc accttttaac tacagcgatg gtgacattgg tctgtttggg cttgcgggag
    11641 ctgccggagc gttgggcgct cgtccggcgg gcggttttgc cgacaagggc aaatcgcacc
    11701 acaactttcg gtctgcttct gctgttactt tcatggctgg caatctggtt tggtcacact
    11761 tccgtactgg cgttgattat cggcattctg gtgctggacc tcaccgtaca gggcgtgcat
    11821 atcactaacc agacggtaat ttatcgaata catcctgatg cgcgtaatcg cctgaccgca
    11881 ggttacatga ccagctactt tattggcggt gccgccggtt cgctaatttc agcctcagcc
    11941 tggcaacatg gcggttgggc tggcgtttgt ctggctggcg cgacgattgc cctggttaac
    12001 ttactggtct ggtggcgagg ttttcatcgt caggaagccg caaattaagc aatcagcaac
    12061 cgttttcgtg gctttacact tataagggtg ttaagaagcc catcagtctg ataaggttaa
    12121 gatattcatt cagtctattt ataatattaa caatcgttaa gcgtacactc tatggaaagc
    12181 cctactccac agcctgctcc tggttcggcg accttcatgg aaggatgcaa agacagttta
    12241 ccgattgtta ttagttatat tccggtggcc tttgcgttcg gtctgaatgc gacccgtctg
    12301 ggattctctc ctctcgaaag cgtttttttc tcctgcatca tttatgcagg cgcgagccag
    12361 ttcgtcatta ccgcgatgct ggcagccggg agtagtttgt gggttgctgc actgagcgtc
    12421 atggcaatgg atgttcgcca tgttttgtat ggcccgtcac tgcgtagccg tattattcag
    12481 cgtctgcaaa aatcgaaaac cgccctatgg gcgtttggcc tgacggatga ggtttttgcc
    12541 gccgccaccg caaaactggt acgcaataat cgccgctgga gcgagaactg gatgatcggc
    12601 attgccttca gttcatggtc atcgtgggta tttggtacgg taataggggc attctccggc
    12661 agcggcttgc tgcaaggtta tcccgccgtt gaagctgcat taggttttat gcttccggca
    12721 ctctttatga gtttcctgct cgcctctttc cagcgcaaac aatctctttg cgttaccgca
    12781 gctttagttg gtgcccttgc aggcgtaacg ctattttcta ttcccgtcgc cattctggca
    12841 ggcattgtct gtggctgcct cactgcgtta atccaggcat tctggcaagg agcgcccgat
    12901 gagctatgag gttctgctgc ttgggttact ggttggcgcg gcgaattatt gcttccgcta
    12961 tttgccgctg cgcctgcgtg tgggtaatgc ccgcccaacc aaacgtggcg cggtaggtat
    13021 tttgctcgac accattggca tcgcctcgat atgcgctctg ctggttgtct ctaccgcacc
    13081 agaagtgatg cacgatacac gccgtttcgt gcccacgctg gtcggcttcg cggtactggg
    13141 tgccagtttc tataaaacac gcagcattat catcccaaca ctgcttagtg cgctggccta
    13201 tgggctcgcc tggaaagtga tggcgattat ataacccaca agaatcattt ttctaaaaca
    13261 atacatttac tttatttgtc actgtcgtta ctatatcggc tgaaattaat gaggtcatac
    13321 ccaaatggat agttcgttta cgcccattga acaaatgcta aaatttcgcg ccagccgcca
    13381 cgaagatttt ccttatcagg agatccttct gactcgtctt tgcatgcaca tgcaaagcaa
    13441 gctgctggag aaccgcaata aaatgctgaa ggctcagggg attaacgaga cgttgtttat
    13501 ggcgttgatt acgctggagt ctcaggaaaa ccacagtatt cagccttctg aattaagttg
    13561 tgctcttgga tcatcccgta ccaacgcgac gcgtattgcc gatgaactgg aaaaacgcgg
    13621 ttggatcgaa cgtcgtaaaa gcgataacga tcgccgctgc ctgcatctgc aattaacgga
    13681 aaaaggtcac gagtttttgc gcgaggtttt accaccgcag cataactgcc tgcatcaact
    13741 ctggtccgcg ctcagcacaa cagaaaaaga tcagctcgag caaatcaccc gcaaattgct
    13801 ctcccgtctc gaccagatgg aacaagacgg tgtggttctc gaagcgatga gctaacgcgt
    13861 catctcgctc aaaaatccag atttataaaa gaaaaaatga ccggccagca tcgcaacatg
    13921 ctggcctttt tggcaagcag gtcggctcag ccgatgagtt aagaagatcg tggagaacaa
    13981 tatgagcgca aatgcggaga ctcaaacccc gcagcaaccg gtaaagaaga gcggcaaacg
    14041 taagcgtctg ctcctccttc tcaccttgct ctttataatt attgccgtag cgatagggat
    14101 ttattggttt ttggtactgc gtcatttcga agaaaccgat gacgcatacg tggcagggaa
    14161 tcagattcaa attatgtctc aggtgtctgg cagcgtgacg aaagtctggg ccgataacac
    14221 cgattttgta aaagaaggcg atgttctggt cactctcgac ccgacagatg ctcgccaggc
    14281 gtttgaaaaa gccaaaactg cactggcttc cagcgttcgc caaacccacc agctgatgat
    14341 taacagcaaa cagttgcagg cgaatattga ggtgcaaaaa atcgccctcg cgaaagcaca
    14401 aagcgactac aaccgccgtg tgccgctggg caatgccaac ctgattggtc gcgaagagct
    14461 gcaacacgcc cgcgacgccg tcaccagtgc ccaggcgcaa ctggacgtcg ctattcaaca
    14521 atacaatgcc aatcaggcga tgattctggg gactaaactg gaagatcagc ctgccgtgca
    14581 acaggctgcc accgaagtac gtaacgccta gctggcgctg gagcgtaccc gtattgtcag
    14641 tccgatgacc ggttatgttt cccgtcgtgc cgtgcaaccg ggtgcgcaaa ttagcccaac
    14701 gacgccgctg atggcggtcg ttccagccac caatatgtgg gtggatgcca actttaaaga
    14761 gacgcagatt gccaatatgc gcatcggtca gccggtcacc atcaccaccg atatttacgg
    14821 cgatgatgtg aaatacaccg gtaaagtggt tggtctggat atgggcacag gtagcgcgtt
    14881 ctcactgctt ccggcgcaaa atgcgaccgg taactggatc aaagtcgttc agcgtctacc
    14941 tgtgcgtatc gaactggacc agaaacagct ggagcaatac ccgctgcgca ttggtttgtc
    15001 cacgctggtg agcgtcaata ccactaaccg tgacggtcag gtactggcaa ataaagtacg
    15061 ttcaacaccg gtcgcggtaa gtaccgcgcg tgaaatcagc ctggcacctg tcaataaact
    15121 gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg atgcaacagc
    15181 aaaaaccgct ggaaggcgcg caactggtca ttatgacgat tgcgctgtca ctggcgacat
    15241 tcatgcaggt gctgaactcc accattgcta acgtggcgat ccccactatc gccgggaatc
    15301 tgggctcatc gctcagccaa ggaacgtggg taatcacttc tttcggggtg gcgaatgcca
    15361 tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa ctgttccttt
    15421 ggtccaccat cgcctttgct attgcgtctt gggcgtgtgg tgtctccagc agcctgaata
    15481 tgctgatctt cttccgcgtg attcagggga ttgtcgccgg gccgttgatc ccgctttcgc
    15541 aaagtctatt gctgaataac tacccgccag ccaaacgctc gatcgcgctg gcgttgtggt
    15601 cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct cggcggttat atcagcgata
    15661 attaccactg gggctggata ttcttcatca acgtgccgat tggcgtggcg gtggtgttga
    15721 tgacactgca aaccctgcgc ggacgtgaaa cccgcaccga acggcggcgg attgatgccg
    15781 tggggctggc actgctggtt attggtatcg gcagcctgca gattatgctc gaccgcggta
    15841 aagagctgga ctgtttttca tcacaggaaa ttatcattct taccgtggtg gcggtggtgg
    15901 ctatctgctt cctgattgtc tgggagctga ccgacgataa cccgatagtc gatctgtcgt
    15961 tgtttaagtc gcgcaacttc accatcggct gcttgtgtat cagcctcgcg tatatgctct
    16021 acttcggcgc tattgttctg ctgccgcagt tgttgcagga ggtctacggt tacacggcga
    16081 cctgggcagg tttggcctct gcgccggtag ggattattcc ggtgattctg tcgccgatta
    16141 tcggccgctt cgcgcataaa ctggatatgc ggcggctggt aaccttcagc tttattatgt
    16201 atgccgtctg cttctactgg cgtgcctata cctttgaacc aggtatggat ttttgcgcgt
    16261 cggcctggcc gcagtttatc caggggtttg cggtggtctg cttctttatg ccgctgacca
    16321 ccattacgct gtctggtttg ccaccggaac gactggcggc ggcatcgagc ctctctaact
    16381 ttacgcgaac gctggcgggg tctatcggca cgtcgataac cacgaccatg tggaccaacc
    16441 gcgagtcgat gcaccatgcg cagttgactg agtcggtaaa cccgttcaac ccgaatgccc
    16501 aggcgatgta cagtcaactg gaagggcttg ggatgacgca acagcaggca tcaggctgga
    16561 ttgcccagca gatcaccaat caggggctga ttatttccgc caatgagatc ttctggatgt
    16621 cagccgggat attcctcgta ctgctggggc tggtgtggtt tgctaaaccg ccatttggcg
    16681 caggtggcgg cggaggcggt gcgcactaag tacaactaag ccagttcatt tgaactggct
    16741 tttttcaatt aattgtgaag atagtttact gactagatgt gcagttcctg caacttctct
    16801 ttcggcagtg ccagttcttc gttgctgttg atgcgtacct cacgttccag aatgctacgc
    16861 gcaatatcct gcgcttcctg caacgagtgc atctggtaag tgccacactg gtagacgttc
    16921 agttccggga tctgattctg atcctgcact ttcagcacgt cttccattgc cgctttccag
    16981 gcatcagcaa cacgctgctc atctggcgta ccaatcagac tcatataaaa accggtgcgg
    17041 cagcccattg gcgagatatc gataatctct acaccattac cgttaagatg gttacgcata
    17101 aaaccagcaa acaggtgctc cagggtatgg atccctcttt ctggcatcac ttctttgttc
    17161 ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc catgcggggt gttcattgtt
    17221 ttcgccaccc gaactgcagg cgcttccatc cgggtatgat cgactgtgaa gctatctaac
    17281 aacggcattt agccacctcc ggtaattttt ttaaaaattt tctgaactct ttcttcccag
    17341 gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc atccctgaat tcagagatga
    17401 aattttggcc actcccgagt ggcctttttc ttttctgtca ggcgtgtttt tccagccaca
    17461 ccgcaaacgg ttcggtatca gcggtttcca tttcctgctg acggcgttca gatgcctcgc
    17521 gctcggctac aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc agcagattac
    17581 ggtaggcttc tgcaaacgct ttgcctgttc cgccaatacc agtatcaatc atagacctta
    17641 agatacgggc agagaaagtc agatcgggat tatcgaagca ggcaaccagt tcatcacaca
    17701 ctttctgata cgcttcgccg ccgttaatgc tatccagcgt ttgcgcgacg cgtttcagat
    17761 cgcggaacag atctttaccc acctgcagta acgggaactg cgcggtttcg cagccgatac
    17821 ccagcgtcag gcccggtttg cgaccttcaa ggatcacccg gttccagtta acgcgcgtac
    17881 aggcaagttc gctactgctc atttccggtg catcagccag cgcacaccag accataaaca
    17941 ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg cgagaacggg ttgatgtcca
    18001 gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa ggtgactcgc
    18061 cgctgcgggt aacgcgtttt ggacgaatcg gcgcatacag ttcgttttca atctgcagca
    18121 cgttgctatt gatttgcagc ctcttaccgt ctttctcaat accaatcttc gcgtactctt
    18181 ccgatggcgt cttgattgcc tgtttaaggc ccgctacgta ctcgtaaaga tcgttgaagg
    18241 taataccaag attgctttgc gatttattgg tatagccgag atcgctcaaa cgaagagagg
    18301 tcgcatacgg caggtaatac ataccgcact cggttttctc aaacggcagc gacgttggtt
    18361 ttccttgcag gaaagaagaa caaatcgccg gagatgcacc aaacagataa ggaatgaccc
    18421 aaccgaaacg atagtaattg cggataacgc ggaaatagcc cgcagaaatt ttctctttgg
    18481 catcagcgcc cgagatatca ccgcacttcg cttgccagaa tgccattggc aaagagaaat
    18541 tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta gcgatttttc agcccttcac
    18601 gatacagcgt tttaaagcgt ccggtgttag aagtgccgta ctgtgccagt tcgatgtcct
    18661 gaccttctgc gatgtagcat ggcatactta acggccacat ccgctcatcg cccatattgc
    18721 gcgccgtata acgatgcaga tcgcgcataa aggtcagcat atgttcaata tcaccatcca
    18781 ctggtgtaat gaattccagc aatgcttccg caaaatcggt agtaatccat ttgtgcgtca
    18841 gtgcggaacc taatgcttca ggatgacctg ttgttgccag tgtgccatca gcattaacac
    18901 gcaaagtttc gcgctccagc ccacgctgta tcccctttaa cgcctgagga tgtttttcca
    18961 gccaggccag cgcctgtgat acgtccggga tcaaattgac ctcccgcctg tcaaaatcgt
    19021 tttaattagc ataactgtaa tggtgaccat atgtgcaggc ctacaattag tgccaccaca
    19081 tcatgccctg aacggtcgct gctgcaactg caacatagcg taacgcttta ccaaggcaca
    19141 aaaaaaagat tactggcccc caggagatgc gcatccatcc cgctaacaga cacagtaaat
    19201 cgccaaccac gggcatccag cttaataata gcgtgactgc accatagcgt ttcagccagc
    19261 cggtagcttt ctcttgccag cgcgatgttt tacgcaatgg aaagaaacgc ccaaggataa
    19321 cgttagttaa ccctccaagg ctattaccca ttgttgctgt taaaactaaa acccagggat
    19381 gactgatccc ggaaagcaac attgccacca gcacgacttc ggagttgccg ggtaatagtg
    19441 tagcgctgag aaaactactg gcaaacaacg agaaaagcga taacgcttca ctcacagcaa
    19501 gcgaacatcc acagcgtcca tgcctgctgc acgggccgcc tgaataccga aatcggcatc
    19561 ttcaaagacc acacactgcg tcggttgcac gcccatacgc tgcgcgcaca acaaaaatgt
    19621 gtctggcgcg ggtttatggt gtttgacgtg atcagcagcg acgacggcgt caaaataacg
    19681 gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg ctttcactcc ccgttcctac
    19741 agccattggg cgacgaccat gccaactttt caccacttca acaagaggaa gcggctcgac
    19801 actatccagc agcatacttc ttactgcttc tgttttttca cgcgctaacg catgcgggtc
    19861 gagatcggcc tgattcagct caataattgc ctgagcaata cgccaggtgg gcgatccatt
    19921 aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag tgccctaata cttcgcgcca
    19981 cgctttacgg tgcgtaggct ccgtatccag gattgtgcca tccatatcaa aaattaaacc
    20041 tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta ctttagcgta
    20101 attgcttgat tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg gataaagtag
    20161 ttatttagaa atgagatatt tttgaaggaa attttttgga gaatatggtg catccgggag
    20221 gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat
//
LOCUS       DAJVPP010000075        20276 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVPP010000075 DAJVPP010000000
VERSION     DAJVPP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.075"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..434)
                     /locus_tag="OQM34_002731"
     CDS             complement(<1..434)
                     /locus_tag="OQM34_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS1453111.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKI"
     gene            complement(489..1340)
                     /gene="manZ"
                     /locus_tag="OQM34_002732"
     CDS             complement(489..1340)
                     /gene="manZ"
                     /locus_tag="OQM34_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1453112.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1353..2153)
                     /gene="manY"
                     /locus_tag="OQM34_002733"
     CDS             complement(1353..2153)
                     /gene="manY"
                     /locus_tag="OQM34_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1453113.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2216..3187)
                     /gene="manX"
                     /locus_tag="OQM34_002734"
     CDS             complement(2216..3187)
                     /gene="manX"
                     /locus_tag="OQM34_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1453114.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3650..5200
                     /gene="yoaE"
                     /locus_tag="OQM34_002735"
     CDS             3650..5200
                     /gene="yoaE"
                     /locus_tag="OQM34_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1453115.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5204..6802)
                     /locus_tag="OQM34_002736"
     CDS             complement(5204..6802)
                     /locus_tag="OQM34_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1453116.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6933..8297)
                     /gene="sdaA"
                     /locus_tag="OQM34_002737"
     CDS             complement(6933..8297)
                     /gene="sdaA"
                     /locus_tag="OQM34_002737"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1453117.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8481..9059)
                     /locus_tag="OQM34_002738"
     CDS             complement(8481..9059)
                     /locus_tag="OQM34_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1453118.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9063..10424)
                     /gene="pabB"
                     /locus_tag="OQM34_002739"
     CDS             complement(9063..10424)
                     /gene="pabB"
                     /locus_tag="OQM34_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1453119.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10498..10677
                     /locus_tag="OQM34_002740"
     CDS             10498..10677
                     /locus_tag="OQM34_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1453120.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10797..11156)
                     /locus_tag="OQM34_002741"
     CDS             complement(10797..11156)
                     /locus_tag="OQM34_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS1453121.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11518..11862)
                     /locus_tag="OQM34_002742"
     CDS             complement(11518..11862)
                     /locus_tag="OQM34_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1453122.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11994..13904
                     /locus_tag="OQM34_002743"
     CDS             11994..13904
                     /locus_tag="OQM34_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1453123.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13962..14657
                     /gene="tsaB"
                     /locus_tag="OQM34_002744"
     CDS             13962..14657
                     /gene="tsaB"
                     /locus_tag="OQM34_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1453124.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14697..15278
                     /gene="yeaY"
                     /locus_tag="OQM34_002745"
     CDS             14697..15278
                     /gene="yeaY"
                     /locus_tag="OQM34_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1453125.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15483..17168
                     /gene="fadD"
                     /locus_tag="OQM34_002746"
     CDS             15483..17168
                     /gene="fadD"
                     /locus_tag="OQM34_002746"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1453126.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPELVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17250..18365
                     /gene="rnd"
                     /locus_tag="OQM34_002747"
     CDS             17250..18365
                     /gene="rnd"
                     /locus_tag="OQM34_002747"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1453127.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18419..19384)
                     /gene="yeaX"
                     /locus_tag="OQM34_002748"
     CDS             complement(18419..19384)
                     /gene="yeaX"
                     /locus_tag="OQM34_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1453128.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19440..20057)
                     /gene="yeaW"
                     /locus_tag="OQM34_002749"
                     /pseudo
     CDS             complement(19440..20057)
                     /gene="yeaW"
                     /locus_tag="OQM34_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         4996 a         5071 c         5259 g         4950 t
ORIGIN      
        1 taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg ctgttctaat
       61 tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct atattcaatc
      121 cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat gcggatccag
      181 aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat tagcgcgcca
      241 tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat aacgctatcg
      301 atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg gcggggcatg
      361 atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat cagtaccagg
      421 tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc cggtagtgta
      481 caacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat gacgaagaag
      541 ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca agcaaatgtc
      601 agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac ggtggtgaca
      661 cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt gacatgtgtc
      721 cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc ttccgtcagt
      781 ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga gtaaccatac
      841 gctacgccgt agtaacgggt tgccagacgc actaggttaa acaggatgaa gaacagcagc
      901 ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa taccggacgt
      961 acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa accgactttg
     1021 ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc ttccagcgcc
     1081 agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa ctccaggtga
     1141 cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg aattgccggt
     1201 accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga accctggaag
     1261 aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt ctcggtggta
     1321 gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc gagatcgttg
     1381 ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct aagttggatg
     1441 tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa gttggtgaat
     1501 gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg catcatgttg
     1561 ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag accattggtc
     1621 accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac agacagcgca
     1681 acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga aacgtggatc
     1741 caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg ctggaaagca
     1801 acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag agggattgcc
     1861 agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt agaaatgata
     1921 gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc cagcgcgatc
     1981 atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac gatacccacc
     2041 agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga tcccatacct
     2101 gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc cattgtactt
     2161 ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca atacgttact
     2221 tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa accttacgga
     2281 cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct ttttcatcaa
     2341 ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca ccgacgttaa
     2401 cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg ttggtaaata
     2461 acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc attttggcaa
     2521 catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt gtcttacgaa
     2581 cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg gtttctttgg
     2641 tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca aggccaataa
     2701 ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt ggtgctgcgg
     2761 cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc acgccttcac
     2821 ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca tcacgggcca
     2881 ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa tgctctttgt
     2941 cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca acgagaaaca
     3001 gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca atcagcgttt
     3061 cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg cctaacagca
     3121 tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata acaatagcaa
     3181 tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat gtatttttga
     3241 atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc cctgtgtttc
     3301 gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt aatttaactt
     3361 tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg caaaggaagg
     3421 taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta acaaccatgg
     3481 agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc atcaggcctt
     3541 ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt gtcgttacgc
     3601 ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca tggaattctt
     3661 aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa ttgtgctggg
     3721 tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa aacaacgcga
     3781 taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc tgctgtcgct
     3841 gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt tctccttctc
     3901 cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag caacaaccga
     3961 actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag gctacgccag
     4021 tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt tggatgcggt
     4081 aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg tagtgattgc
     4141 gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc agcaccccac
     4201 ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg tggcagaagg
     4261 tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct cgatcattat
     4321 cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga ctttgccgct
     4381 gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc aggccaatgt
     4441 tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg aagaagaacg
     4501 gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga tcatgacgcc
     4561 gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc gcgagcaact
     4621 gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg aaatcatcgg
     4681 tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg tggcggcgat
     4741 tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca acctgctggg
     4801 cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg gtgtggtaca
     4861 aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc cggacgctga
     4921 cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg gtacagattt
     4981 gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg atatcgcgac
     5041 ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg atgtgattga
     5101 tgtagggcca ctgcatatca ccatccttga agccaatgat tatcgtgttg atctggttcg
     5161 cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt aacgacataa
     5221 tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc agccatttgg
     5281 gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata tgcacgccat
     5341 gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc agttcaatat
     5401 tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc gcgtcagaac
     5461 ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat gtttcaagcc
     5521 aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg tttttacgat
     5581 gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg gtgatttcga
     5641 ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct tttatcagca
     5701 caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga ctactcatcg
     5761 ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt ggcacaatta
     5821 aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc tgacgcggat
     5881 tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca ttaagcaaag
     5941 gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg cgggaaaagc
     6001 tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt agcagcaccg
     6061 ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc ggaccattta
     6121 cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg tcttcaggtt
     6181 gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc accgtcaggc
     6241 tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt aagtcgatgt
     6301 taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat tgaatcaaaa
     6361 ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt tgcggtgcag
     6421 gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg ctggagcaat
     6481 aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt gccgcctgtt
     6541 tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc aagactttgc
     6601 tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga ttggcgaatt
     6661 gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc agggtcgaag
     6721 cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga taggttttaa
     6781 tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac aaacgcctga
     6841 cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg tataaattcg
     6901 cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt gattgccaga
     6961 ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc gtacatcgtt
     7021 tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc catccgtgcg
     7081 gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac ctgaacctgc
     7141 cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc cgccgccacg
     7201 caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc cattgaacag
     7261 gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc gttcatttta
     7321 tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc tgggctgacc
     7381 gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca ggcaccgttg
     7441 gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc cagcgcaaac
     7501 atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct ggaaactaac
     7561 atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag tacaccttcg
     7621 gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc gaaatactcg
     7681 tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc agacagcgaa
     7741 tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg atacggcacg
     7801 ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa accgccgccg
     7861 atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg gatttgcata
     7921 ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc gcgcgggaaa
     7981 tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac gtcgcgaata
     8041 aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag acccataata
     8101 atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata aacgtccacg
     8161 gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac gaactgttta
     8221 cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac cttaaacatg
     8281 tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca gtaacgaact
     8341 aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc acatgaatta
     8401 aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt agtgttttgt
     8461 agatgtaaag ataagtatag tcaggggttc acaccaattt gcagcgccag ctcacgaatt
     8521 atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca tacccgatgt
     8581 gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa tgcctgggcg
     8641 agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg cagatcgggc
     8701 gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac gggcggcagc
     8761 acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc ttcgcgcagc
     8821 gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa tgccacttgt
     8881 ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg ttgcggtcga
     8941 cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg gttaatttgc
     9001 gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg gtattccacg
     9061 tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc ctgatattcc
     9121 gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat ttgtccgtta
     9181 atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa gctcaaatag
     9241 ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat aatttccata
     9301 gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg cagcagatcg
     9361 ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg atgcacggca
     9421 gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac ggcaacacga
     9481 ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc tttcgctgag
     9541 ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg tggtagcgtg
     9601 cctttaatcg ggcgggtctg gatttcacta ttatcacaaa gaataaaccg ctctggcgaa
     9661 aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc gcggttggcc
     9721 tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg aaaacgctgg
     9781 gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg gcgaaatttt
     9841 tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt gaaatcttcc
     9901 tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc attatgactc
     9961 agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta gatacccact
    10021 gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc aaaacggcgg
    10081 cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc ttcgttatgc
    10141 gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg gtcatcagtg
    10201 gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt ggttaaagtg
    10261 caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc atagccggag
    10321 tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc agcggcgtcc
    10381 tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc tgactctact
    10441 ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga gttaacaatg
    10501 tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga gcggatccag
    10561 gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc agaagagctg
    10621 cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga cgagtaacac
    10681 actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga gaaaaaggac
    10741 gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa tttaactcag
    10801 accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt tttaatcaca
    10861 gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt gaacccaggg
    10921 ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg tactcccagt
    10981 tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact tggtgttggc
    11041 gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc aggcatattc
    11101 ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat ttgcatctaa
    11161 tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag gtgttttaaa
    11221 ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc agcccgcacg
    11281 ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa gggcgaacac
    11341 aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga aattataaat
    11401 ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt cgatttatgt
    11461 aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg ataaagctta
    11521 caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac ccgcttgtac
    11581 cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt tattcatcgc
    11641 cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg atttattgct
    11701 gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt gctcaaaggc
    11761 atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt ggattactac
    11821 atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt cctcattata
    11881 cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc acttcaactg
    11941 gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt agtgtgacgg
    12001 acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag ccgcgagaac
    12061 cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag ccgctggtgg
    12121 tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg ctgcgggcga
    12181 aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc tacagccgcg
    12241 atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg ctgaaagggc
    12301 gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg ggcgatctgc
    12361 cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa acagtcgatg
    12421 gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg ctggtcacca
    12481 gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc tttgtggtca
    12541 aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat ctctttctgg
    12601 cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg gacgtcatga
    12661 tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt cagtcactct
    12721 ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc accgaattaa
    12781 aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg caggattttc
    12841 gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta gctaatccgc
    12901 aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat gatgtggcga
    12961 aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc acgttgtatc
    13021 gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac tggtacgaat
    13081 gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat aaattcaaag
    13141 agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg tcggtgaacg
    13201 acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg ttgctattgc
    13261 caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat ctgccgcaaa
    13321 ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc atcgaagcta
    13381 acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat ctggccgagc
    13441 agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt aaaggccaac
    13501 tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt agtttctggg
    13561 aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa ttgccgttta
    13621 cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg cgcggtggcg
    13681 acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag ggggtaggtc
    13741 gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat cggctggtga
    13801 tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc acccgtgaca
    13861 ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt acggagtatg
    13921 gtatgatgcg cgcattttta ttgatctaag cacgaagacc catgcgaatt ctggctatcg
    13981 ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc aacgctcatt
    14041 ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag gatatcctga
    14101 ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc ggccccggta
    14161 gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt ggcgcggaat
    14221 taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg cgcaaaaacg
    14281 gcgcaacacg cgtgctgtca gccattgacg cgcgaatggg cgaagtttac tgggccgaat
    14341 atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta ctcaaacccg
    14401 aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta gggacgggct
    14461 ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat ggcgaagtgt
    14521 tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc gagggtaaaa
    14581 cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca tggaagaaac
    14641 ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt cgcatcatgg
    14701 cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta atgctgagcg
    14761 gttgtgtcac tgtgccggac gccattaaag gcagcagtcc cacgccgcaa caagatttag
    14821 ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt ggtggcaaag
    14881 tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc gtaccgctgg
    14941 acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat gccgatgtga
    15001 acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc gggccaatca
    15061 ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg atgcaagtaa
    15121 cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag ccgattgatc
    15181 catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc tggtataatc
    15241 ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt aaagaaaaag
    15301 aaacagcggc tggtccgctg tttctgcatt cttacggtaa agataaaaat aaatagtgac
    15361 gcgcttcgca accttttcgt tgggtaatta tcaagctggt atgatgagtt aatattatgt
    15421 taacagcgtg tatatcattt ggggttgcga tgacgacgaa cacgcatttt agaggtgaag
    15481 aattgaagaa ggtttggctt aaccgttatc ccgcggacgt tccgacggag atcaaccctg
    15541 accgttatca atctctggta gatatgtttg agcagtcggt cgcgcgctac gccgatcagc
    15601 ctgcgtttgt gaatatgggg gaggtgatga ccttccgcaa gctggaagaa cgcagtcgcg
    15661 cgtttgccgc ttatttgcaa caagggttgg ggctgaagaa aggcgatcgc gttgcgttga
    15721 tgatgcctaa cttattgcaa tatccggtgg cgctatttgg cattttgcgt gccgggataa
    15781 tcgtcgtaaa cgttaacccg ttgtataccc cgcgtgagct tgagcatcag cttaacgata
    15841 gcggcgcatc ggcgattgtt atcgtgtcta actttgccca cactctggaa aaagtggttg
    15901 ataaaaccgc cgttcagcac gtaattctga cccgtatggg cgatcagcta tctacggcaa
    15961 aaggcacggt agtcaatttc gttgttaaat acatcaagcg cttggtgccg aaataccatc
    16021 tgccagatgc catttcattt cgtagcgcac tgcataacgg ctaccggatg cagtacgtca
    16081 aacccgaact ggtgccggaa gatttagcct tcctgcaata caccggcggc accacaggtg
    16141 tggcgaaagg cgcgatgctg actcaccgca atatgctggc gaacctggaa caggtcaacg
    16201 ccacctatgg gccgttgctg catccaggta aagaactggt agtaacggca ctgccgctgt
    16261 atcacatttt tgccctgacc attaactgcc tgctgtttat cgaactgggt gggcagaacc
    16321 tgcttatcac taacccgcgt gatattccag agttggtaaa agagttagcg aaatatccgt
    16381 ttaccgctat cactggcgtt aacaccttgt tcaatgcgtt gctgaacaat aaagagttcc
    16441 agcagctgga tttctccagt ctgcatcttt ccgcaggcgg tgggatgcct gttcagcaag
    16501 tggtggcaga acgctgggtg aaactgaccg gacagtatct gctggaaggt tatggcctga
    16561 ccgagtgcgc gccgctggtg agcgttaacc cgtatgatat tgattatcat agcggtagca
    16621 tcggtttgcc ggtgccatcg acggaagcca aactggtgga tgacgacgac aacgaagtac
    16681 caccgggtca accgggtgag ctttgtgtca aaggaccgca ggtgatgctg ggttactggc
    16741 agcgtccgga tgctacagat gagatcatca aaaatggctg gttacacacc ggcgacatcg
    16801 cggtgatgga tgaagaagga ttcctgcgca ttgtcgatcg taaaaaagac atgattttgg
    16861 tttccggttt taacgtctat cccaacgaga ttgaagatgt cgtcatgcag catcctggcg
    16921 tacaggaagt cgcggctgtt ggcgtacctt ccggctccag tggtgaagcg gtgaaaatct
    16981 tcgtagtgaa aaaagatcca tcgcttaccg aagagtcact ggtgactttt tgccgccgtc
    17041 agctcacggg atacaaagta ccgaagctgg tggagtttcg tgatgagtta ccgaaatcta
    17101 acgtcggaaa aattttgcga cgagaattac gtgacgaagc gcgcggcaaa gtggacaata
    17161 aagcctgagc gttaagtcag tcgtcagacg ccggttaatc cggcgttttt tttgacgccc
    17221 actaaagaga aaacaatttg aattaccaaa tgattaccac ggacgatgcg ctggcttctt
    17281 tgtgtgaagc cgtccgtgtc tttccggcga tagccctgga tactgaattt gttcgtacgc
    17341 gcacttatta cccgcagctg gggttgattc aacttttcga tggcgagcat ctggcgctaa
    17401 ttgatcctct cgggatcacc gactggtcac cgctgaaagc gatcctgcgc gatccgtcca
    17461 tcacaaaatt tctccatgca ggcagtgaag atctggaagt gttcctcaat gtctttggcg
    17521 aattaccaca acccttgatt gacacgcaaa tccttgctgc cttctgcgga cgcccgatgt
    17581 catggggttt cgcttccatg gtggaagagt attccggcgt tacgctggac aagagtgaat
    17641 cgcgcaccga ctggctggcc agaccgctga ccgaacgtca gtgtgaatac gcagcggcgg
    17701 atgtctggta tctgttaccg atcaccgcca agcttatggt agaaacggag gcctccggct
    17761 ggctacctgc ggcgctggat gaatgccgcc tgatgcaaat gcgtcgtcag gaagtcgttg
    17821 cgccggaaga tgcctggcgt gatatcacca atgcctggca gttacgcaca cgccaactgg
    17881 cctgtctgca actgttagcc gactggcgac tgcgcaaggc gcgagagcgt gatctggcgg
    17941 tgaactttgt cgtgcgtgaa gagcatttgt ggtcggtcgc gcgttatatg ccgggaagtt
    18001 taggcgaact ggacagcctg ggtttatccg gtagcgaaat ccgctttcac ggtaaaacgc
    18061 tgctggcgct ggtggaaaaa gcgcagacat tgccggaaga tgccttaccg cagccgatgc
    18121 ttaacctgat ggacatgcct ggttatcgta aagcgtttaa agcgattaag tcgctgatta
    18181 ctgacgtgag cgaaacgcat aagatcagcg ccgaattgct ggcatcgcgt cggcaaatca
    18241 accaactgct gaactggcac tggaaactga aaccgcagaa caatttgccg gagctgattt
    18301 ccggctggcg tggtgagctg atggcggaag cattacacaa tttattgcag gaatatccgc
    18361 agtaaaatct tccgaagccg gactgggcgc gctcagcgcc acatccggct ttggcaaact
    18421 acaaatccaa caccaggcgt ttacccttcg cacgcgaaca acagatcaac atactttgct
    18481 ggctggcacg ctcttcatcg ctaaaatact gatcccgatg gtcagcttca ccttccagta
    18541 ttgccgtttc gcaggttccg catacccctt cacgacataa acattccact ttcgcggctt
    18601 tattattttc aataacctgc aaaatagtca tctcttccgg caccacaaac tcttttccgg
    18661 aacgggcaag caccagggta aatgcatcgc cggttttgtc ttcgatagca aattgctcaa
    18721 agtgcagtgt atcggcggcg atgtccagac gcgcagcttc acttcttacc gcttcaatta
    18781 gcgcctcggg gccacaggta taaacgtgtg tgccaggttc gatatccgcc aataatcgcg
    18841 ctaattccag tcgtgttccg gttgatgaat gatgcaaatg gactttctca gcctgcggat
    18901 gctggactag ctcagcacga aatgcgcaac tttctggatt tcgcgagcag taatgtagct
    18961 gccagtcgat gtcgctgtgt tgcaactctg ccatgtgcga caggaaaggg gtgataccaa
    19021 taccgcccgc gataaacaga tgctttctgg ctgagggaat tagcgcaaac aggttattag
    19081 gcgttgagat cgttaaccga tcgcccactt ttacctgctg atgcaaaaag cgggaaccgc
    19141 cgcgcgagtt ttcttccagt cgaacggcaa tctgataact agaggtgtta tgcggtgaac
    19201 tcagtagtga atacgcattg ctgtactggt tatcaccatc gctcatctgc actaggatgt
    19261 gacttcctcc ggtgaacgca ggcagcggtt tgccatcggt tgccaccagc gtgaagcgtt
    19321 tcacctgttc ggtaaggggt tcaacctggc tcacctgtac ttcaaacatt tgatagtctg
    19381 acattgctgt actccatcaa aaatcagccc agcggcggaa aataccgccg ccgatgtcat
    19441 tagtccttaa acacctgcgc cagcagatta tggaaatggg cgataccatg ttcggaaatg
    19501 ccactaccgc tactgtcggc catgatgcgc ccctgaccac gatagccacg cgatttcagc
    19561 cctttctgta cgctttcaac cagacgtaaa tcttccggat ggaacacatc gcgataccac
    19621 tcaatcagcg atttttgctc gtcggttaac tcttcattgg tgaagtaaat atcgtagttt
    19681 tgcagggtag tttcagaatc caccgggaat tcataaatga ccgtcatcat ccctttgatc
    19741 ggggtgacgt tcagcatcgt gcacggccac agccagaaac cgtggaatgc cgcatccgta
    19801 ccctcttcaa atttaaacga ctgttcggac ggtttggcga aaccgtattg cagcgtccag
    19861 ttaccgtgca tggtgtgcca gtaacgatca acctgtacgg agtcggagaa acctagatgc
    19921 gccggaccac agtgatagca ctcgagatag ttatcgacaa tgttcttcca gttggcaggc
    19981 gtgcgggtgg taaagcgggc cgctagtttc agattgtgga cttccgggca ggcttccagc
    20041 actttcgcgc ccagaccggt gtattgattc acttgaagta cgaaaaaaaa ccgggaggac
    20101 attggattat tcgggttctg atgggattag atttggtggg gcttgcaagc ctgtagcgca
    20161 aattttagtc gttaatcaat gaaacgcgaa agatagtaaa aaattgcttt tgtttcattg
    20221 aaaatacgaa aaacaaaaac actgcaaatc atttcaataa cagcttcaaa aaacgt
//
LOCUS       DAJVPP010000076        20216 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVPP010000076 DAJVPP010000000
VERSION     DAJVPP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.076"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1487
                     /locus_tag="OQM34_002750"
     CDS             <1..1487
                     /locus_tag="OQM34_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1453129.1"
                     /translation="DLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKSTP
                     TLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETFLP
                     ELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFM
                     GFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNA
                     PHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTIILF
                     TFDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYP
                     TALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNY
                     HKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLA
                     AANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1539..2696
                     /locus_tag="OQM34_002751"
     CDS             1539..2696
                     /locus_tag="OQM34_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1453130.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2662..2784)
                     /locus_tag="OQM34_002752"
     CDS             complement(2662..2784)
                     /locus_tag="OQM34_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453131.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2987..4672
                     /locus_tag="OQM34_002753"
                     /pseudo
     CDS             2987..4672
                     /locus_tag="OQM34_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4710..7070
                     /locus_tag="OQM34_002754"
     CDS             4710..7070
                     /locus_tag="OQM34_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1453132.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7127..9910
                     /locus_tag="OQM34_002755"
     CDS             7127..9910
                     /locus_tag="OQM34_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1453133.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSHLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10271..11671
                     /locus_tag="OQM34_002756"
     CDS             10271..11671
                     /locus_tag="OQM34_002756"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1453134.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            11827..13362
                     /gene="gadC"
                     /locus_tag="OQM34_002757"
     CDS             11827..13362
                     /gene="gadC"
                     /locus_tag="OQM34_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1453135.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            13493..14812
                     /locus_tag="OQM34_002758"
     CDS             13493..14812
                     /locus_tag="OQM34_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1453136.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            15110..15151
                     /locus_tag="OQM34_002759"
     CDS             15110..15151
                     /locus_tag="OQM34_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453137.1"
                     /translation="MVQLVDLARCVSF"
     gene            15189..16324
                     /gene="dosC"
                     /locus_tag="OQM34_002760"
                     /pseudo
     CDS             15189..16324
                     /gene="dosC"
                     /locus_tag="OQM34_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            16249..16866
                     /locus_tag="OQM34_002761"
                     /pseudo
     CDS             16249..16866
                     /locus_tag="OQM34_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            17124..17456
                     /gene="ddpX"
                     /locus_tag="OQM34_002762"
                     /pseudo
     CDS             17124..17456
                     /gene="ddpX"
                     /locus_tag="OQM34_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            17470..18366
                     /locus_tag="OQM34_002763"
     CDS             17470..18366
                     /locus_tag="OQM34_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1453138.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            18363..19349
                     /locus_tag="OQM34_002764"
     CDS             18363..19349
                     /locus_tag="OQM34_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1453139.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            19342..20216
                     /locus_tag="OQM34_002765"
                     /pseudo
     CDS             19342..20216
                     /locus_tag="OQM34_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         5443 a         4717 c         4759 g         5297 t
ORIGIN      
        1 atgatcttgg ttatggacaa cttccttttg ataagggatc ttttgaccca aaaacaatgg
       61 aaaatcgtga agttgtcgat acctacaaaa tagggataga taaagccatt gaagctgcac
      121 aaaaatcaac gccgacgctc ctttcattaa tggatgaagg cgtacgtttt actaacggct
      181 atgtggcaca cggtgtttcc ggcccctccc gtgccgcaat aatgaccggt cgagctcccg
      241 cccgctttgg tgtctattcc aataccgatg ctcaggatgg tattccgcta acagaaactt
      301 tcttgcctga attattccag aatcatggtt attacactgc agcagtaggt aaatggcact
      361 tgtcaaaaat cagtaatgtg ccggtaccgg aagataaaca aacgcgtgac tatcatgaca
      421 acttcaccac attttctgcg gaagaatggc aacctcaaaa ccgtggcttt gattacttta
      481 tgggattcca cgctgcagga acggcatatt acaactcccc ttcactgttc aaaaatcgtg
      541 aacgtgtccc cgcaaaaggt tatatcagcg atcagttaac cgatgaggca attggcgttg
      601 ttgatcgtgc caaaacactt gaccagcctt ttatgcttta cctggcttat aatgctccgc
      661 acctaccaaa tgataatcct gcaccggatc aatatcagaa gcaatttaat accggtagtc
      721 aaacagcaga taactactac gcttccgttt attctgttga tcagggtgta aaacgcattc
      781 tcgaacaact gaagaaaaac ggacagtatg acaatacaat tattctcttt accttcgata
      841 atggtgcggt tatcgatggt cctctgccgc tgaacggggc gcaaaaaggc tataagagtc
      901 agacctatcc tggcggtact cacaccccaa tgtttatgtg gtggaaagga aaacttcaac
      961 ccggtaatta tgacaagctg atttccgcaa tggatttcta cccaacagct cttgatgcag
     1021 ccgatatcag cattccaaaa gaccttaagc tggatggtgt tcccttgctg ccctggttgc
     1081 aagataagaa acaaggcgag ccacataaaa atctgacctg gataacctct tattctcact
     1141 ggtttgatga ggaaaatatt ccattctggg ataattacca caaatttgtt cgccatcagt
     1201 cagacgatta cccgcataac cccaacactg aggacttaag ccaattctct tatacggtga
     1261 gaaataacga ttattcgctt gtctatacag tagaaaacaa tcagttaggt ctctacaaac
     1321 tgacggatct acagcaaaaa gataaccttg ccgccgccaa tccgcaggtc gttaaagaga
     1381 tgcaaggcgt ggtaagagag tttatcgaca gcagccagcc accgcttagc gaggtaaatc
     1441 aggagaagtt taacaatatc aagaaagcac taagcgaagc gaaataacta aaccttcatg
     1501 cggcggattt ttccgccgcc ttattgagcg agatagcgat gcacgttaca gccaagccct
     1561 ccagttttca atgtaatctc aaatgtgatt actgttttta ccttgaaaaa gagtcgcagt
     1621 ttactcatga aaaatggatg gatgacagca ctctgaaaga gttcatcaaa caatatatcg
     1681 aagcgtctgg caatcaggtc tattttacct ggcaaggcgg tgaacccact ctggctggcc
     1741 tggatttttt ccgtaaagtt attcactatc aacaacgcta tgcaggccaa aaacgtattt
     1801 ttaatgcatt acaaacgaat ggcattttat tgaataatga atggtgtgcc tttctcaaag
     1861 aacatgaatt tctggttggt atctcgatcg atggccccca ggagttacat gaccgttaca
     1921 gacgcagtaa ttcaggtaac ggtacttttg caaaagtgat agcagccatc gagcgtctga
     1981 aatcatatca agtagagttt aatacgttaa ccgtcattaa taacgttaat gtccattatc
     2041 ctcttgaggt ttatcatttt ttaaaatcta tcggcagtaa acatatgcaa tttatcgaat
     2101 tgctagaaac cgggacgccg aatattgatt tcagtggtca tagtgagaac acattccgta
     2161 tcattgattt ttctgtgcct cccacggctt atggcaagtt tatgtcaacc atttttatgc
     2221 aatgggttaa aaacgatgtg ggtgaaattt tcattcgtca gtttgaaagc tttgtcagcc
     2281 gttttttggg gaatgggcat acaagttgta ttttccagga gtcctgcaag gataatctgg
     2341 ttgttgaaag taatggagac atttacgaat gcgaccattt tgtctatcca cagtacaaaa
     2401 ttggaaacat taataaatct gaactcaaaa cgatgaacag tgtacaactg acagcgcaaa
     2461 aaaaacggat ttcagcgaaa tgtcagcaat gtgcatataa acctatctgc aatggcggtt
     2521 gtcctaagca tcgtattact aaagtaaaca atgagactgt ttcttatttt tgcgaaggtt
     2581 ataaaatcct tttttcaacc atggtacctt atatgaacgc catggttgag ttagctaaga
     2641 acagagtacc gctttaccac attatggatg ttgcaaaaca aatggagaat aattaataac
     2701 gctctctttt aactccattc ttagcctcct tcttatgacc ttaacaaggc acccgacata
     2761 aattcctgtc gggtgtttcg tcatcatcat cttcccttgc gaaaatcctg gcttacgtgg
     2821 tatgcgtcag ggcattagca atacccttga ttatcaatag cataaccata accactgaaa
     2881 attaaaaatt tctatatatt tcatattgtt aaatgatgct taaataaatt tcatcagtaa
     2941 atcattgcca tagataatca ttggatggca caatcaattt tcgctaatga taaccattcc
     3001 cattacgttt tgtatgttga tagcaaagta tctctgtctg ctgaagccct tttggctgcg
     3061 taagaataat aaaacttcag tgttgttaat cattattatc ctcgcaatga tccttggcgt
     3121 ggttaaaatt caggtctggt taaacgactg gaataatgat tttttcaatg cgctaagcca
     3181 gaaagaaacc gataagcttt ggcagctcgt tctttggttc cctgccctgc tggggatctt
     3241 tgtattaata tccgtaaaca aaacatggtt aattaaacta ctgactattc gctggcgcga
     3301 atggttaaca gattactacc ttaatcgctg gtttgcagat aaaaactact acttcacgca
     3361 gatctacggc gagcataaaa atacagataa ccctgaccaa cgtatcgctg aagacattct
     3421 cttactgatc aacaaaacgc tcagtctttc atttggcttc atccagtcac ttagcatgct
     3481 gatcaccttt accgttattc tttggcaaag cgcgggtacg ctctcattca ctgttggtgg
     3541 aacagaatgg aatatccagg ggtatatggt ctataccgtc gtgctcattg tgatcggtgg
     3601 aactttattt acccataagg tgggtaaacg tattcgtccg cttaatgtgg aaaaacaacg
     3661 tagcgaagcg acatttcgaa ctaatcttgt gcagcataat aagcaggcag aactgattgc
     3721 gctaagcaat gctgagtctc tacaacgcca ggagttgcgc gacaattttc ataccattaa
     3781 agagaactgg catcgtttaa tgaatcgtca gcggtggctt gattactggc aaaatattta
     3841 ttcgcgctcg cttagcgttc ttccctactt tctgttattg ccgcagttta ttagcgggca
     3901 gatcaatctg ggcggactga tgaaatcgcg ccaggcattt atgctagtat cgaacaattt
     3961 aagctggttt atttataaat atgacgaact tgctgaactg gctgcggtta tcgatcgctt
     4021 gtatgagttc catcaactca ctgaatagcg ccctacgaat aagcctaaaa attgccaaca
     4081 tgcggtacaa gtggctaatg cgagtattcg tacgcctgat aataagatca tattagagaa
     4141 cttgaacttt catgtttcgc caggcaaatg gctattactg aaaggctact ctggtgcggg
     4201 aaaaaccaca ctgcttaaaa cattatccca ctgctggccg tggtttaaag gtgatatttc
     4261 ttctcctgct gacagttggt atgtgtcaca aacaccgtta atcaaaagcg gcttactgaa
     4321 agagattatt tgtaaagcac ttcccctgcc cgtagacgat aaatcgttga gcgaagtact
     4381 gcatcaggtt ggtctgggaa aattggctgc gcgtattcat gaccacgatc gctggggaga
     4441 tattctttcc agcggcgaaa aacaacgtat cgccctggca cgattaattt tacgacgtcc
     4501 gaaatggata tttcttgacg aaactacctc tcatcttgag gaacaagagg ctatacgctt
     4561 actgcgttta gtgcgtgaaa aactacccac aagcggcgtc attatggtta cacatcaacc
     4621 cggtgtctgg aacctggctg atgatatttg tgacattagc acggttttat aacaacaata
     4681 aaagccctgc tgtacggaga tataaataaa tgaagcgagt tcttattcct ggcgtcattt
     4741 tatgtggcgc tgatgtggcg caggccgtcg atgacaaaaa catgtacatg tatttttttg
     4801 aagagatgac ggtctatgct cctgtccctg tacccgtaaa cggcaacacg cattacacca
     4861 gtgaaagcat cgagcgttta ccgaccggga atggcaatat cagcgatctg ctgagaacca
     4921 accctgcggt acgcatggat tcaacgcaaa gtacctcgtt gaaccaggga gatattcgcc
     4981 ctgagaaaat ctctattcac ggtgcgtcgc cctaccagaa tgcctatttg attgacggta
     5041 ttagtgcaac taataacctg aacccagcga atgagtccga tgccagtagt gcaaccaata
     5101 ttagcgggat gtcacagggg tattatcttg atgtcagctt actggacaat gtgacgcttt
     5161 atgacagttt tgtgccggtt gaatttggtc gcttcaatgg cggggtaatt gatgcaaaga
     5221 tcaaacgctt caacgctgat gatagcaagg tgaaattggg ttatcgcact acgcgtttgg
     5281 actggttaac atcgcatatc gatgagaata acaagagcgc atttaatcaa ggttcttcag
     5341 gaagtaccta tttctctcca gattttaaaa agaactttta taccttgtcg tttaatcagg
     5401 agctggctga taactttggc gttaccgccg gtttatcgcg ccgccagtct gatatcaccc
     5461 gcgcggatta tgtttcgaat gacggcattg tcgccggtcg ggcacagtat aaaaacgtta
     5521 tcgatactgc attgagcaaa tttacctggt ttgccagcga ccgctttacc cacgatttaa
     5581 ccttaaaata taccggctcc agccgtgatt ataataccag caccttcccg cagtctgatc
     5641 gcgaaatggg taataaatcc tatggtctgg catgggatat ggatacgcaa ctcgcatggg
     5701 ccaaactacg taccaccgtt ggttgggatc atattagtga ttatacccgt cacgatcatg
     5761 acatctggta caccgaactt tcatgtacat atggtgatat tacagggcgt tgtacccgtg
     5821 gcggattagg acacatttcc caggctgtag ataattacac cttcaaaaca cgcctggact
     5881 ggcaaaaatt cgccgtgggt gatgtttcgc atcaacccta cttcggcgcg gaatacatct
     5941 attccgatgc atggactgaa cgccataacc agtctgaatc ctatgtgatt aatgctgcag
     6001 gaaagaaaac taaccatacc atttaccata aaggtaaagg cagcctggga attgacaact
     6061 acacgctgta tatggcggat cacattagct ggcggaatgt gtcgttaatg cccggtgtgc
     6121 gttatgacta tgacaactat ctgtcaaacc acaatatctc cccgcgcttt atgacggaat
     6181 gggatatttt tgctgatcaa acctcaatga ttaccgccgg ttataaccgt tactatggcg
     6241 ggaatattct tgatatggga ttacgtgata tccgcaatag ctggacggaa tcggtatcag
     6301 gtaataaaac cctgacgcgt tatcagaatt tgaaaacgcc ttataacgat gaactggcaa
     6361 tgggattgca gcaaaaaatc gataagaacg ttattgcacg cgcaagtgaa gcgcatgatc
     6421 aaatcagcaa aagcagtcgt accgacagcg cgactaaaac caccattact gaatataaca
     6481 acgatggcaa aaccaaaacg cattcgttta acctcagttt tgaactggcc gaacccctgc
     6541 atatccgcca ggtagatatt aacccgcaaa ttgtctttag ctatatcaag agcaagggca
     6601 acttgtcgtt aaacaatggt tatgaggaga gcaataccgg tgataaccag gtggtttata
     6661 acggtaatct ggtctcttac gatagcgttc cagtggcaga ttttaataac ccattaaaga
     6721 tctccttaaa catggatttc acgcatcaac cgagcgggtt agtgtgggcg aatacgctgg
     6781 cctggcaaga agcgcgtaaa gctcgcatta tccttggtaa gacaaatgcg caatacatca
     6841 gcgaatattc agattacaag cagtatgttg acgaaaaact ggatagcagc ctgacctggg
     6901 acacccgctt gtcctggacg ccacaatttc tgaaacaaca aaacctgacg atcagtgccg
     6961 atattctcaa tgtactggat agcaaaaccg ctgttgatac aacgaacacc ggtgtggcga
     7021 cctacgccag tggccgtact ttctggcttg atgtcagcat gaaattttaa tagttcggat
     7081 tttacttaac cgggtaaatt cgcccggttt tcgcatggag ataattatga gaaacctctg
     7141 tttcttactg acgttagtgg caactctgtt gctccccggg cggctgattg ccgccgcctt
     7201 accgcaggat gaaaagttaa ttaccgggca actggacaat gacttgcgat atatgattta
     7261 tccgcatgct catccaaagg atcaggtaaa tttatggctg caaattcata ccggttcatt
     7321 gcaggaagaa gacaatgagc gcggcgtggc tcattttgta gaacatatga tgtttaacgg
     7381 cacaaaaaca tggccgggta ataaagtcat cgaaacattt gagtcaatgg gcctgcgttt
     7441 tggtcgcgat gttaatgcct ataccagcta tgacgaaacg gtgtatcagg tgagtttgcc
     7501 gactacgcag aaacaaaatc tgcaacaagt gatggcaatc ttcagtgaat ggagtaatgc
     7561 cgcaaccttt gaaaaactcg aagtagacgc tgaacgtggc gtaattactg aggaatggcg
     7621 tgcccatcag gatgcgaaat ggcgcacctc tcaggcgcgc cgccctttcc tgctggctaa
     7681 tacccgtaat ttagaccgtg aacctatcgg cctgatggat actgtcgcca cggtcacgcc
     7741 ggcacaattg cgccaatttt atcaacgctg gtatcaacca aataatatga cctttatcgt
     7801 ggtcggcgat atcgacagta aagaagcgct ggcgctgata aaggataatt taagtaagct
     7861 tccggctaac aaagcagctg aaaatcgcgt ctggccgaca aaagccgaaa accacctgcg
     7921 ctttaatatc attaatgata aagaaaaccg ggtgaacggc atcgcactct attatcgcct
     7981 gccaatggta caagtgaacg atgagcaaag ctttgtcgaa caagctgaat ggagcatgtt
     8041 agttcagctg ttcaatcaac gtctgcagga acgcatacag tcgggcgagt tgaagactat
     8101 ttctggcggc actgcgcgca gcgttaaaat tgcacccgat tatcagtcgc tgtttttccg
     8161 tgtaaatgca cgagacgata atatgcagga cgctgcgaat gcattaatgg cagagttggc
     8221 aaccattgat cagcatggtt tttctgctga agaactcgat gatgtcaaat ctacccgcct
     8281 cacctggctg aaaaatgcgg ttgatcagca agctgaacgt gatttacgta tgctgaccag
     8341 tcacctggca tccagctcat taaataatac gccgttcttg tcgccggaag agacatatca
     8401 actttcgaaa cgtctgtggc agcaaattac cgtgcaaagt ctggcggaaa aatggcagca
     8461 gttaagaaag aaccaggacg cattttggga gcaaatggta aacaatgagc ttgccgccaa
     8521 aaaagcattg tctcctgcgg ctatcctggc gctggaaaaa gagtacgcca acaaaaagct
     8581 ggcggcttac atcttcccag gcagaaattt atcgttaaca gtagacgctg acccacaggc
     8641 ggaaattagc agcaaagaaa cgctggctga gaatctgaca tcattaacac tttccaatgg
     8701 tgccaaggtt attctggcaa aatccgcggg tgaagagcaa aagctacaaa ttattgccgt
     8761 atcgaataaa ggcgatttaa gtttccctgc gcagcaaaaa tcacttatcg cgctggcaaa
     8821 taaagcagtt agcggaagcg gcgttggcga actctcctct tccagcctga aacgctggag
     8881 tgcggaaaac tcggtaacca tgagcagtaa agtcagtggc atgaatacgt tgctctccgt
     8941 tagcgcgcgg actaataacc ctgaacctgg tttccagttg attaaccagc gaatcaccca
     9001 cagcacgatt aacgacaata tttgggcatc gctacaaaat gctcaaattc aggcgttgaa
     9061 aacgctcgac cagcgtccag cggagaaatt cgcccagctg atgtatgaga cgcgctatgc
     9121 tgatgaccgc acgaaattac tgcaagaaaa tcagattgca cagtttactg ccgcagatgc
     9181 gctggctgcc gatcgccagt tgttttcatc tccagcggat atcacgtttg tcattgtcgg
     9241 taatgtcgca gaagacaaac tcgtggcgtt aattacgcgt tacttaggct caatcaaaca
     9301 ctctgattcg ccattagccg caggtaaacc attaactcgc gcgacggaca acgcatcggt
     9361 tactgtaaaa gaacaaaatg aacctgtggc acaggtttca cagtggaagc gttatgattc
     9421 ccggacacct gttaatctgg cgacgcgtat ggcgctcgat gcttttaacg tcgcactggc
     9481 aaaagatcta cgcgttaata ttcgtgaaca ggcatctggg gcatacagcg tttcttctcg
     9541 cctctcggtt gatcctcagg ctaaagatat cagtcatttg ctggctttta cttgtcaacc
     9601 agaacgacat gatgaacagt taacgttagc gaatgaagtg atggttaagc gtttgactaa
     9661 ggggatcagt gagcaagaac tgaacgaata ccagcaaaac gttcagcgca gcctcgatat
     9721 ccaacagcgt agcgttcaac aattagcgaa cactattata aatagtctta ttcaatatga
     9781 cgatcctgca gcatggactg agcaggagca attgttgaaa caaatgacgg tagagaatgt
     9841 taacactgcc gttaaacaat atctttctca tccggtaaat acttataccg gagtattatt
     9901 gccaaaataa taacagcccc gtcaacacat cgttggcggg gattttagca atattcgcta
     9961 tttttatgta ataattttat aaatgcgttc aaaataataa ccaagtacta atagtgatat
    10021 tttaatgcct gatttttacg tgataattca ggaggcacag aaagcgcata aaaataacaa
    10081 cataaaacac cttgccacca cccaagaatt ctctattaca ttgtttttaa tgaaaaaata
    10141 ccattccaac aatatctcac gataaataac attaggattt tgttatttaa acacgagtcc
    10201 tttgcacttg cttactttat cgataaatcc tactttttta atgcgatcca atcattttaa
    10261 ggagtttaaa atggataaga agcaagtaac ggatttaagg tcggaactac tcgattcacg
    10321 ttttggtgcg aagtctattt ccactatcgc agaatcaaaa cgttttccgc tgcacgaaat
    10381 gcgcgacgat gtcgcatttc agattatcaa tgatgaatta tatcttgatg gcaacgctcg
    10441 tcagaacctg gccactttct gccagacctg ggacgacgaa aacgtccaca agttgatgga
    10501 tttatccatt aacaaaaact ggatcgacaa agaagaatat ccgcaatccg cagccatcga
    10561 cctgcgttgc gtaaacatgg ttgccgatct gtggcatgcg cctgcgccga aaaatggtca
    10621 ggccgttggc accaacacca ttggttcttc cgaggcctgt atgctcggcg ggatggcgat
    10681 gaaatggcgt tggcgcaagc gtatggaagc tgcaggcaaa ccaacggata aaccaaacct
    10741 ggtgtgcggt ccggtgcaaa tctgctggca taaattcgcc cgctactggg atgtggagct
    10801 gcgtgagatc cctatgcgcc ccggtcagtt gtttatggac ccgaaacgca tgattgaagc
    10861 ctgcgacgaa aataccatcg gcgtggtgcc gactttcggc gtgacctaca ccggtaacta
    10921 tgagttcccg caaccgctgc acgatgcact ggataaattc caggccgaca ccggtatcga
    10981 catcgacatg cacatcgacg ccgccagcgg tggctttctg gcaccgttcg tcgccccgga
    11041 tatcgtctgg gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag gccataaatt
    11101 cggtctggct ccgctgggct gcggctgggt tatctggcgt gacgaagaag cgctgccgca
    11161 ggaactggtg ttcaacgttg actacctcgg tggtcagatt ggtacttttg ccatcaactt
    11221 ctcccgcccg gcgggtcagg tgattgcaca gtactatgaa ttcctgcgcc ttggtcgtga
    11281 aggctatacc aaagtacaga atgcttccta ccaggtcgct gcctatctgg cggatgagat
    11341 cgccaaactg gggccgtatg agttcatctg taccggtcgc ccggacgaag gcatcccggc
    11401 ggtttgcttc aaactgaaag atggtgaaga tccgggatac accctgtacg acctctctga
    11461 acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg aagccaccga
    11521 catcgtggtg atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg ctgaactgtt
    11581 gctggaagac tacaaagcct ccctgaaata tctcagcgat cacccgaaac tgcagggtat
    11641 tgcccagcag aacagcttta aacatacctg ataacgttta acggtaacgg tgtcccgaaa
    11701 cgaacccgtt tcgggataat ttccaaagtc tgttcactgg cattagcaac ggaaaatatt
    11761 gttctgaata cgcttcagaa caaaacaggt gcggttccga caggaatacc gttttagggg
    11821 gataatatgg ctacatcagt acagacaggt aaagctaagc agctcacatt actcggattc
    11881 tttgccataa cggcatcgat ggtaatggct gtttatgaat accctacctt cgcaacatcg
    11941 ggcttttcat tagtcttctt cctgctatta ggcgggattt tatggtttat tcccgtggga
    12001 ctttgtgctg cggaaatggc caccgtcgac ggctgggaag aaggtggtgt cttcgcctgg
    12061 gtatcaaata ctctgggtcc gagatgggga tttgcagcga tctcatttgg ctatctgcaa
    12121 atcgccattg gttttattcc gatgctctat ttcgtgttag gggcactctc ctacatcctg
    12181 aaatggccag cgctgaatga agaccccatt accaaaacta ttgcagcact catcattctt
    12241 tgggcgctgg cattaacgca gtttggtggc acgaaataca cggcgcgaat tgctaaagtt
    12301 ggcttcttcg ccggtatcct gttacctgca tttattttga tcgcattagc ggctatttac
    12361 ctgcactccg gtgcccccgt tgctatcgaa atggattcga agaccttctt ccctgacttc
    12421 tctaaagtgg gcacactggt tgtatttgtt gccttcattt tgagttatat gggcgtagaa
    12481 gcttccgcaa ctcacgtcaa tgaaatgagc aaccccgggc gcgactatcc actggctatg
    12541 ttactgctga tggtggcggc aatctgctta agctctgttg gcggtttgtc tattgcgatg
    12601 gtcattccgg gtaatgaaat caacctctcc gcaggggtaa tgcaaacctt taccgttctg
    12661 atgtcccatg tggcaccgga aattgagtgg acggttcgcg tgatctccgc actgctgttg
    12721 ctgggtgttc tggcggaaat cgcctcctgg attgttggtc cttctcgcgg gatgtatgtc
    12781 acagcgcaga aaaacctgct gccagcggca ttcgctaaaa tgaacaaaaa tggcgtaccg
    12841 gtaacgctgg tcatttcgca gctggtgatt acttctatcg cgttgatcat cctcaccaat
    12901 accggtggcg gtaacaacat gtccttcctg atcgcactgg cgctgacggt ggtgatttat
    12961 ctgtgtgctt atttcatgct gtttattggc tacattgtgt tggttcttaa acatcctgac
    13021 ttaaaacgca catttaatat ccctggtggt aaaggggtga aactggtcgt ggcaattgtc
    13081 ggtctgctga cttcaattat ggcgtttatt gtttccttcc tgccgccgga taacatccag
    13141 ggtgattcta ccgatatgta tgttgaatta ctggttgtta gtttcctggt ggtacttgcc
    13201 ctgcccttta ttctctatgc tgttcatgat cgtaaaggca aagcgaatac cggcgtcact
    13261 ctggagccaa tcaacagtca gaacgcacca aaaggtcact tcttcctgca cccgcgtgca
    13321 cgttcaccac actatattgt gatgaatgac aagaaacact aaccatgtct gaatggttat
    13381 aagacaaggg agcgataatt catcgctccc tttttcgtgc ttgctgtcgt attgacttaa
    13441 ccgggataag tacgagaatg agcgcacatc tgtttaccgg aaaccagcac atatggatat
    13501 ctgctcccga aacaagaaat taacgattag aagaccagcg atactggttg cattggcact
    13561 tttactgtgt agttgtaaaa gcacgcctcc agagtccatg gtgacaccac cagcaggttc
    13621 aaagccacca gccacgacgc aacaatcgtc acaaccgatg cgtggcatct ggctggccac
    13681 ggtttctcgc ctcgactggc caccggtttc ctcggttaac attagtaatc ccaccagccg
    13741 agcccgtgta caacaacagg cgatgatcga caaactggat catctccaac gtctcggcat
    13801 aaacacggtc tttttccagg tcaagccgga cggtaccgcc ctgtggccat cgaaaatttt
    13861 gccgtggtcc gatcttatga ccggtaagat tggtgaaaat ccgggttacg atccgctgca
    13921 attcatgctc gacgaagccc acaagcgtgg gatgaaagta cacgcctggt ttaaccccta
    13981 tcgcgtatcg gttaatacga agcccggtac tatcagggaa ctgaatagca ctctgtctca
    14041 acaaccggcg agcgtctatg tgcaacaccg tgactggatc agaacgtctg gcgatcgctt
    14101 tgtcctcgac ccgggcatcc ctgaggttca ggactggatc acatcaatag tcgctgaagt
    14161 ggtttcccgc tatccggtag atggcgtgca gtttgacgac tatttctata ctgaatcacc
    14221 gggttcacgg ctaaatgata acgaaacgta ccgtaaatac ggaggcgcat ttgcgtcaaa
    14281 agcagactgg cggcgcaaca atactcagca gttaattgca aaggtatcac acaccattaa
    14341 aagcattaag ccgggagtcg aatttggcgt tagcccggca ggcgtgtggc gtaaccgatc
    14401 acacgatccg ctcggttccg atacccgagg cgcggcagcc tatgacgaat cctacgcaga
    14461 cacccgtcga tgggtggaac aaggattgct ggattacatt gctccccaaa tttactggcc
    14521 gttctcacgg agtgccgcgc gttatgacgt gttggcaaaa tggtgggcgg atgtcgttaa
    14581 accaaccagg acccgcctgt atatcggtat cgccttctat aaagtgggtg aaccttcaaa
    14641 gatagagcca gactggatga ttaacggcgg cgtaccggaa ctgaaaaagc agctcgatct
    14701 taacgatgcg gtaccagaaa ttagcggcac catcttgttc cgtgaggact atctgaataa
    14761 accgcagact caacaagcgg tcagctatct gcaaagtcgt tggggcagtt aaactaccat
    14821 gcttactttg taacaagccg gtgctttacc caccggcttg ttacaccttg tgaaatatcc
    14881 cactagtaat catgcttaca taagtcaaat taaccacgca attaaccaca cttagttaaa
    14941 cagctataaa tccctaataa aaatgtatga atatgaaaaa ccatacgata ttcgctgtca
    15001 ccgaaaaata attacgatgg gctatactct tctgaagaac agtagcgtag attgaaactg
    15061 tcttaagtcg cttatttctt ttcagtatat cttcatattt caggagaata tggtgcagtt
    15121 agtcgatctc gcacgttgcg tttcgttttg attccgtctg atttcccagc cttataaggg
    15181 tgtgaattat ggagatgtat tttaaaagaa tgaaagatga gtggaccgga cttgtcgaac
    15241 aggcagatcc tctcattcgt gctaaagccg cagaaattgc ccttgcgcat gctcattatc
    15301 tgagtatcga gttttatcga attgtccgca tcgacccgca tgccgaagaa ttcttgagta
    15361 atgaacaagt tgagcggcag ttgaaaagtg cgatggaacg ctggattatt aacgtgcttt
    15421 ctgcccaggt tgacgatgtc gaaaggctaa tacaaatcca gcataccgtc gcggaagtgc
    15481 atgcccgcat aggaattccg gtagaaattg tcgagatggg gtttcgggtg ctgaaaaaaa
    15541 tcctctatcc ggtaatcttc tcttcggatt attccgccgc agaaaaactt caggtctacc
    15601 atttctcgat taacagtatt gatatcgcga tggaagtgat gacccgcgcg tttaccttta
    15661 gtgacagtag tgcctcaaag gaagatgaaa actatcgtat cttctcgtta ctggaaaacg
    15721 ccgaagaaga aaaagaacgg caaatagcct caatactttc atgggaaata gatattatct
    15781 ataaagtcct gctggattct gatttaggca gcagtttgcc tttaagccag gctgattttg
    15841 gcctgtggtt taacgacatt attttagcgg cattgccgaa gtgggccata tctcccgtct
    15901 gattcaggat ttcgacggta ttttcaatca aaccatgcgt aacaccagga ttttgaataa
    15961 cagaagtttg cgggtgaaat ttctattaca gataagaaat accgtatcgc agattattac
    16021 cttgctgcgt gaattgtttg aagaagtatc gcgccacgaa gtcggtatgg atgtactgac
    16081 gaaattactt aaccgccgtt tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa
    16141 ccggaccggt acaccgctgt cagtgctgat tattgacgtt gataaattca aagagatcaa
    16201 cgatacgtgg ggccataaca ctggcgatga aattctgcgt aaagtctcat tcctctcgca
    16261 gaagagattg gtgaaatcga aaatattggg cgctgggtca tcgcggaagc ttgccgtcag
    16321 ttagcagaat ggcgtagcca gaatattcat atcccggcgt tatccgtgaa cttgtcggcg
    16381 ctgcactttc gcagtaatca gctgcctaat caggtgtctg atgcaatgca agcctggggt
    16441 attgacggcc accagctgac ggtggaaatc acggaaagca tgatgatgga acacgatacc
    16501 gaaatcttta agcgcattca gatcctgcgt gatatgggcg tgggtttatc ggtagatgat
    16561 tttggcacgg gcttttccgg attatcccgc ttagtcagtc ttccggtaac ggaaatcaaa
    16621 attgacaaaa gctttgtcga tcgttgtctg acggaaaaac gcatcctggc cttacttgaa
    16681 gccattacca gcattgggca aagcctcaat ttaaccgtcg tggcggaagg ggtcgaaacc
    16741 aaagagcaat ttgagatgct acgtaagatc cactgtcgcg ttattcaggg atatttcttt
    16801 tcccgccccc ttcccgccga agaaattcca ggctggatga gcagcgtgtt accgctgaaa
    16861 atctgacaaa ttcctctcgc ccgcactcgc gggttttcct ttaaggtgac actgtcactt
    16921 aaatactgtg atttcagcta cgattccggg agattccttc cttaactttc cctcttgcac
    16981 caaaactgca ctacgctgca caaatgttga tcaaaagtta aaaaatataa ctttaagcca
    17041 gcattctggc gcttatcccg gcatggcatg agatctgcat aagcggaaag cgcagcaatt
    17101 tttgtcttat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
    17161 tcttccctga tctggagatc gaattgaaat atgcctgcgt tgataacatc acaggtaaag
    17221 ctatttatca gcaagcgcgt tgtctgctac ataaagatgc gattaccgcg ctggcgaaaa
    17281 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
    17341 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
    17401 cggtcggttc taatcacagc cgtggcacgg cgctcgacct gacgcttcgt gatgacgtgg
    17461 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    17521 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    17581 gtcatcattg tattaatgcg actgatgatg attttttcac cgtggctggc gacgcatgat
    17641 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    17701 accgatgaag tgggacgcga tctgtttagc cgcgtactgg tcggcagtca gcaatcaatt
    17761 ctcgccggat tagtggtggt cgccattgcg ggtatgattg gttcgctact cggatgtcta
    17821 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    17881 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    17941 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgctt ggcgcggggg
    18001 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggcgcgtct
    18061 cgttggcatc tgatcaactg gcatatttta cgtaactccc taccgccgct gatcgtgcag
    18121 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggccta
    18181 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag cgaatggtcg caactatgta
    18241 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac ctccgtcggg
    18301 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    18361 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    18421 gtgatgttca cgcgctcaac aatgtgtcct tgcagattaa ccgcggcgaa attgtcggtc
    18481 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    18541 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagaa gatgttctta
    18601 acgctcggga aaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    18661 aaccgatgac cgcccttaat ccgacacgtc gaataggtct tcagatgatg gacgtaatcc
    18721 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattgcc ctgctggaag
    18781 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    18841 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    18901 ccggcgaacc gacgacggcg ctggacgtca cagtacagtt gcaggtactg cgtttgctta
    18961 aacataaagc acgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    19021 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    19081 ggacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    19141 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    19201 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    19261 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcactgc gcctgttggt
    19321 atccgcagca ggaggtcata agtgtctgac acgttattaa cgttacgcga cgtccatatc
    19381 aattttccgg cccgtaaaaa ctggcttggt aaatctacgg aacatgttca tgccattaat
    19441 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggcga gtcaggctgc
    19501 ggcaaaagca ccctcgcaca gcttttaatg ggcatgctgc aaccgagcca cgggcagtac
    19561 atccgttcag gctcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    19621 aatccgcgct taccggtgtg gcgcatcatc acagaatcgc tctggatagc taagcgtagt
    19681 agtgaacaac agcggcgagc gttggcagag gagttggctg tgcaggtggg tattcgtccg
    19741 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    19801 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    19861 gatatctccg tgcaggcgca gatcctcaat ttgctggtaa cgctacagga aaatcacggg
    19921 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    19981 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcaacaggt gttgactgct
    20041 tcggcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    20101 gaggaagaat gagcattacg taaaacggat ctgccgggaa accgcacgtt gccgcaaggc
    20161 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatc
//
LOCUS       DAJVPP010000077        19972 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVPP010000077 DAJVPP010000000
VERSION     DAJVPP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.077"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            88..429
                     /gene="raiA"
                     /locus_tag="OQM34_002766"
     CDS             88..429
                     /gene="raiA"
                     /locus_tag="OQM34_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1453140.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    532..657
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            533..580
                     /gene="pheL"
                     /locus_tag="OQM34_002767"
     CDS             533..580
                     /gene="pheL"
                     /locus_tag="OQM34_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCS1453141.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            679..1839
                     /gene="pheA"
                     /locus_tag="OQM34_002768"
     CDS             679..1839
                     /gene="pheA"
                     /locus_tag="OQM34_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1453142.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1882..3003)
                     /gene="tyrA"
                     /locus_tag="OQM34_002769"
     CDS             complement(1882..3003)
                     /gene="tyrA"
                     /locus_tag="OQM34_002769"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1453143.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3014..4084)
                     /gene="aroF"
                     /locus_tag="OQM34_002770"
     CDS             complement(3014..4084)
                     /gene="aroF"
                     /locus_tag="OQM34_002770"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1453144.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4294..4659
                     /locus_tag="OQM34_002771"
     CDS             4294..4659
                     /locus_tag="OQM34_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1453145.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTNWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4809..5327
                     /locus_tag="OQM34_002772"
     CDS             4809..5327
                     /locus_tag="OQM34_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS1453146.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5317..6542
                     /gene="dgcN"
                     /locus_tag="OQM34_002773"
                     /pseudo
     CDS             5317..6542
                     /gene="dgcN"
                     /locus_tag="OQM34_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6558..7040
                     /locus_tag="OQM34_002774"
     CDS             6558..7040
                     /locus_tag="OQM34_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1453147.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7117..7464)
                     /gene="rplS"
                     /locus_tag="OQM34_002775"
     CDS             complement(7117..7464)
                     /gene="rplS"
                     /locus_tag="OQM34_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1453148.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7506..8273)
                     /gene="trmD"
                     /locus_tag="OQM34_002776"
     CDS             complement(7506..8273)
                     /gene="trmD"
                     /locus_tag="OQM34_002776"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1453149.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8304..8852)
                     /gene="rimM"
                     /locus_tag="OQM34_002777"
     CDS             complement(8304..8852)
                     /gene="rimM"
                     /locus_tag="OQM34_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1453150.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8871..9119)
                     /gene="rpsP"
                     /locus_tag="OQM34_002778"
     CDS             complement(8871..9119)
                     /gene="rpsP"
                     /locus_tag="OQM34_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1453151.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9256..10617)
                     /gene="ffh"
                     /locus_tag="OQM34_002779"
     CDS             complement(9256..10617)
                     /gene="ffh"
                     /locus_tag="OQM34_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1453152.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10784..11575
                     /locus_tag="OQM34_002780"
     CDS             10784..11575
                     /locus_tag="OQM34_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1453153.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11596..12882
                     /locus_tag="OQM34_002781"
     CDS             11596..12882
                     /locus_tag="OQM34_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1453154.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12937..13530)
                     /gene="grpE"
                     /locus_tag="OQM34_002782"
     CDS             complement(12937..13530)
                     /gene="grpE"
                     /locus_tag="OQM34_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1453155.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13653..14531
                     /gene="nadK"
                     /locus_tag="OQM34_002783"
     CDS             13653..14531
                     /gene="nadK"
                     /locus_tag="OQM34_002783"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1453156.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14617..16278
                     /gene="recN"
                     /locus_tag="OQM34_002784"
     CDS             14617..16278
                     /gene="recN"
                     /locus_tag="OQM34_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1453157.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16427..16768
                     /gene="bamE"
                     /locus_tag="OQM34_002785"
     CDS             16427..16768
                     /gene="bamE"
                     /locus_tag="OQM34_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1453158.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16830..17120)
                     /locus_tag="OQM34_002786"
     CDS             complement(16830..17120)
                     /locus_tag="OQM34_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1453159.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17110..17547)
                     /gene="ratA"
                     /locus_tag="OQM34_002787"
     CDS             complement(17110..17547)
                     /gene="ratA"
                     /locus_tag="OQM34_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1453160.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17718..18200
                     /gene="smpB"
                     /locus_tag="OQM34_002788"
     CDS             17718..18200
                     /gene="smpB"
                     /locus_tag="OQM34_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1453161.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18415..18777
                     /gene="ssrA"
                     /locus_tag="OQM34_002789"
     tmRNA           18415..18777
                     /gene="ssrA"
                     /locus_tag="OQM34_002789"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            18982..>19972
                     /locus_tag="OQM34_002790"
     CDS             18982..>19972
                     /locus_tag="OQM34_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1453162.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGF"
BASE COUNT         5159 a         5251 c         4670 g         4892 t
ORIGIN      
        1 acaaaattat gagatttcca tcacacattt tgacatcagg aacggtatgc tgaattcacc
       61 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
      121 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
      181 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
      241 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
      301 accgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
      361 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
      421 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
      481 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
      541 cataccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
      601 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
      661 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
      721 agcgcgctgg atgaaaaatt attagcgtta ctggcagaac ggcgcgaact ggccgtcgag
      781 gtgggaaaag ccaaactgct ctcgcatcgc ccggtacgtg atattgatcg tgaacgcgat
      841 ttgctggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
      901 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccaacaa
      961 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     1021 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     1081 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     1141 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     1201 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     1261 tccggcacta ctgatttatc caccatcaat acggtctaca gccatccgca gccattccag
     1321 caatgcagca aattccttaa tcgttatccg cactggaaga ttgaatatac cgaaagtacg
     1381 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc
     1441 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc aaatcagcga
     1501 caaaacttca cccgatttgt ggtgttggcg cgtaaagcca ttaacgtgtc tgatcaggtt
     1561 ccggcgaaaa ccacgttgtt aatggcgacc gggcaacaag ccggtgcgtt ggttgaagcg
     1621 ttgctggtac tgcgcaacca caatctgatt atgacccgtc tagaatcacg cccgattcac
     1681 ggtaatccat gggaagagat gttctatctg gatattcagg ctaatcttga atcagcggaa
     1741 atgcaaaaag cattgaaaga gttaggggaa atcacccgtt caatgaaggt attgggctgt
     1801 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     1861 gaatcatccg gcactggatt attactggcg attgtcattc gcctgacgca ataacacgcg
     1921 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     1981 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     2041 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataatgatgt cggcataaag
     2101 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     2161 agagagcgcc agaagttgct caagctgaac attttcttct gccaggtgca gcccgtaagc
     2221 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     2281 gctaatacga tgcaaccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     2341 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     2401 gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa
     2461 tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     2521 tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     2581 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     2641 cgatagtgtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     2701 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     2761 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     2821 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     2881 tccaaagcgg cttttcacct tgcccacttc agcaaccagt tccagacgct tcgctaataa
     2941 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     3001 cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt
     3061 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     3121 atttcatttc actgcgcggt tgctcagaag actgattgcc ctcgtggata ttactttcga
     3181 tcatcagacc aataattgag cgattaccat ctttgatttg agcaaccacg gattctgcca
     3241 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     3301 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     3361 agttcggcgc tttaccaccg cgcaggatca catgcccgtc cggattcccc tgagtttgta
     3421 gcaacgcaac ctgccctgcc tggttaatgc caacaaaaca gtgcggctgg gcggcggcgc
     3481 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     3541 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     3601 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     3661 ccagtggcag tcccatattc accagctcaa gcaacaattt acgcgcgatc tgcagcccgg
     3721 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     3781 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     3841 cggcaagagc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     3901 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttt
     3961 tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt
     4021 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtctttat
     4081 gcatgatggc gatcctgttt atgctcgttt gcgatagttg ctcctcagcg aggatgacgt
     4141 aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta tttacatagg
     4201 caatttagtc gcgctttcaa cccttgcttc tgtatagata aatttacatc ccctttaaaa
     4261 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
     4321 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
     4381 agcaccaact ggtatgatgt cggtatggaa gatgcgatat cgggcagcgc cataaaagat
     4441 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
     4501 gggcaaaaga aaacttgcca gaccgatttt acctatgccc ggggactttc cggtaaaagc
     4561 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
     4621 agggctgatg aaaatgccag cacaatacga ttaaattaat taaaacccca caaataaatt
     4681 aaacataaga ttttactcat tgtgttttat ttctcacatt gatgacggtc gcatgaaaaa
     4741 atgatatttt aatgagcatc ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgggg
     4801 atccctgaat gcgtttttct caccgactgt tcttacttct gattctcctg ctgacgggag
     4861 caccaatact cgctcaagag ccttctgacg ttgcgaaaaa cgtgcgtatg atggtttccg
     4921 gtattgtcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta tgtatctttt
     4981 catcttcacg ttttagtacc gccctacaag agaatgccgc gacatctttg ccttatctgc
     5041 ctgtcatcat tcacactcaa caagaggcga tgatttcagg ctgcaatggt ttttattttg
     5101 gcaacgagtc accgacattc cagatggaat taaccgaaca atatccgtca aaggcgttat
     5161 tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt ctgatcatcc
     5221 acaataatga cgtcagattt gccgtaaacc tggatgcctc atcgcgtagc ggtgtaaagg
     5281 tcaacccgga tgtcttaatg ctcgcacgga agaaaaatga tggataacga taattctctt
     5341 aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag catatttatc
     5401 actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct gaaacagtac
     5461 gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga agcagctgtc
     5521 gtttttgccg atggccctgc agcaactgaa acactggcag cgctgggcca gcaagggcat
     5581 tttcaactgc agaagtacgt gataagcagc aaaatattct ggcatcctgg cattaccccc
     5641 gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc cctgccccca
     5701 tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta accgctcgcg
     5761 acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt tgtattctgc
     5821 tggcatcagg catcgcaatt accctcaccc gccatttgca caatggcctg gtggaagcac
     5881 tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt tcccgacgag
     5941 tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac agtctgctgg
     6001 atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt ctgcgtaccg
     6061 cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc ggcatcaata
     6121 cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt gatggcgata
     6181 atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagggtc ttgattgaaa
     6241 tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc ctgggcggcg
     6301 atgaattcgc tatggtgctc tattatgtac agtcggaatc tgaagtgcag cagatatgct
     6361 cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggccat cagaccacca
     6421 tgacattaag cattggttac gcgatgacca ttgagcacgc ctctgcggaa aaattacaag
     6481 agcttgccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag ctggtgagat
     6541 aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc acctcactaa
     6601 tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc gccgctatgc
     6661 aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca gatgccattc
     6721 tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc caaaccatgg
     6781 cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac accgataact
     6841 atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc gtggccgatg
     6901 catgggctat tggtggacaa attccacgca gcaatctcac cacacagggt ttaggaaaaa
     6961 aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc cgcgtcgcag
     7021 tggtgattac aaccccttaa aacacgagca aacagacaaa aaaagggcca gccaattggc
     7081 cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct ctttgatacg
     7141 agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa cagcaccacg
     7201 acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac gctcaacgcc
     7261 ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt tacgaatagc
     7321 gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa cccatacttt
     7381 cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct gctcttgttc
     7441 aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac tgatctctcg
     7501 ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt tttgaactcc
     7561 gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc aggtcttcta
     7621 agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc atggttgccc
     7681 gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt atagtgcggg
     7741 caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg tcccagtact
     7801 cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc accaccactg
     7861 agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac gcgctcatct
     7921 ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag ttcgctgacg
     7981 cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc gccttcaccc
     8041 gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat taacatcccc
     8101 ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt gaagtcgcga
     8161 ggactccaac tctggatgct cagcaggcca tttttaactg cccggccagt taccccgtaa
     8221 tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca catagcgccg
     8281 ccttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc gattgaacga
     8341 gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag acgttccttg
     8401 ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattaga tccggtttcc
     8461 atcatatcaa cgactttacc gagatcgtag ccttcagtgg ttactacctg gcagcccatc
     8521 aggtctttcc agtagtagtc cccctcttca agctgaggca gctgcgatga atccacgaca
     8581 atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt cagcttgatg
     8641 atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca ctgacccgcc
     8701 ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt ggaagaaaac
     8761 actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac gatgggatca
     8821 acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga ttaagctgct
     8881 ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc ctggccaacc
     8941 cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct agcgattggg
     9001 ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct gtcagcgaca
     9061 acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat agttaccata
     9121 acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc cggtgtcata
     9181 ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt taataactct
     9241 gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcattccct tcatgcttct
     9301 catcatcttc gccattccgc ctttcttcat tttcttcatc atgcgctgca tgtcgtcgaa
     9361 ctgcttcaga aggcggttaa cgtcctgtac ctgcatcccg caaccggcgg cgatacgacg
     9421 cttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca ttgagttgat
     9481 gatggcttcc atacgcacca gcactttatc gtccatctgc gacttgacgt tatccgggat
     9541 ctgccccatg cccggcagtt tgcccatcaa gctggccatg ccgcccatat ttttcatctg
     9601 gcgcagttgc tcaagaaagt cgttgagatc gaagccgtcg ccttttttta gcttgctggc
     9661 taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca gcgacagtac
     9721 gtcgcccatg ccgagaatac gcgacgcgat gcggtccgga tggaacggct ccagcgcctc
     9781 agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac gaatagagag
     9841 cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg taagcggtaa
     9901 cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg cgtcaaccac
     9961 aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt ccatcatcgc
    10021 ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga atttcagttt
    10081 ggcttctttc agcgccgcgt taacaatatc aaccggcttc tgaccaacat cagaagggaa
    10141 gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg ccgggcgata
    10201 gacgtcagca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga aattaccgag
    10261 tttaccaacg ctggttgttt taccggcacc ttgcaggcct gccatcagca cgaccgcagg
    10321 cggttgcgca gccagattca gggtctggtt ctcttcgccc atcgccgcaa ccagttcgtt
    10381 acggacgatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt catgaccaac
    10441 cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga cgtccgcctc
    10501 cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag tgaggcgtcc
    10561 acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat caaacattgt
    10621 ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt attttgccgc
    10681 agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt cacctgacgc
    10741 tatactgctt ctctttctta ttgctcaaac tgtcgacatt actatgcccg tttttgctct
    10801 gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc tgctgcaaaa
    10861 aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg tctgccacgc
    10921 aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc tcagcctgct
    10981 gaacgttggt tcattggtca gtttggtgat ctgtacggta atgaccattg tggcttctcg
    11041 caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca acctggcgct
    11101 ggcgaccttc atgcccaatg aatacatcac ccatctggaa gcaacgcctg ggatgctgat
    11161 gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc tgtacgcgct
    11221 gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcattta accaggaaat
    11281 gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg gcgtggtgct
    11341 gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacctattta gcatggaaaa
    11401 tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc tgctgtgggg
    11461 acattaccat gaaggatggc gtggacgccg cgtcgtctgg tttaacgttg cgggcgcggt
    11521 aattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca gctaaaccca
    11581 gaaaaggagt ttcccctgga acacatttct actactacgt tgatcattat tctgatcatc
    11641 atggtggtca tttcagccta tttttccggg tccgaaaccg gaatgatgac cctcaaccgc
    11701 tatcgtcttc gacatatggc gaaacagggt aatcgctcgg ccaaacgcgt cgaaaaattg
    11761 ctgcgtaagc cagaccgcct gataagcctg gtgttaatcg gcaataacct ggtcaatatt
    11821 cttgcctccg cgctcggcac tattgtcggg atgcgtttgt acggcgatgc gggcgtggca
    11881 attgcgactg gtgtgctgac ttttgtcgta ctggtttttg ctgaggtatt gccgaaaacc
    11941 attgccgcgc tgtacccgga aaaagtcgct tatccgagta gttttctgct ggctccgctg
    12001 caaattttga tgatgccgct ggtctggttg ctgaatgcta tcacccgtat gctgatgcgc
    12061 atgatgggta tcaaaaccga tatcgtggtt agcggctctt tgagcaaaga agagttgcgc
    12121 actatcgtgc atgaatcgcg ctcacaaatt tcccgtcgca atcaggatat gctgctgtcg
    12181 gtgctcgatc tggaaaaaat gaccgttgat gacatcatgg taccgcgcag tgaaattatc
    12241 ggtattgata tcaacgatga ctggaaatct attctgcgcc aactctccca ctcacctcac
    12301 gggcgcatcg tgctttaccg tgattcgctg gacgacgcca tcagtatgct gcgagtacgt
    12361 gaagcctggc ggttgatgtc ggagaaaaaa gagttcacca aagaaaccat gctgcgcgcc
    12421 gcggacgaga tctattttgt gcctgaaggt acgccgctca gcacgcagtt ggtaaagttt
    12481 cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat tcaggggctg
    12541 gtgacggttg aagatattct ggaagagatt gtcggcgatt tcactacgtc gatgtcgcca
    12601 acacttgccg aagaggtcac gccgcaaaac gacggttcgg tgattatcga tggcaccgcc
    12661 aacgtgcggg aaatcaacaa agcctttaac tggcatctac cggaagatga tgcccgcacg
    12721 gttaatggcg tcattcttga ggcactggaa gagatccctg tcgcaggcac ccgcgtgcgt
    12781 attggcaagt acgatatcga tattctcgac gtacaggaca atatgattaa gcaggtaaaa
    12841 gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt aacgaaaacg gccctgcatt
    12901 cgcatgcagg gccgtgaatt attacgaaaa cagaaattac gcttttgctt tcgctacagt
    12961 taccatcgcc gcacgaatcg tacgaccatt cagcgtataa cccttctgca taatgcccag
    13021 tacgttacct ggcgcaacgt catcagattc caccattgcg atagcctgat gcacattcgg
    13081 gtccagcggc acgttagttt cggcgatcac ttcaacacca aacttacgca caacatccag
    13141 catcgacttc agcgtcagct caatgccttc aaccatcgca gacatatccg ggttagcttt
    13201 atcagccact tccagcgcac gatccaggct atcaatcacc ggcagcaatt cgttgatgaa
    13261 tttctccagc gcgaatttgt gggctttttc aatatccagt tcagtacgac gacgcaggtt
    13321 ttccatttcg gcttttacgc gcaaaatacc gtcacgttca cgggtctggg cttcagccag
    13381 ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc acctgctcag cagaagcttc
    13441 tggctcaact gcctcaatct cttcgtgctg atccatgata atttcttccg gggcttgccc
    13501 ctcaggcgtt ttctgttctt tactactcat gaatttctcc gcgttttttt cgcattcatc
    13561 tcgctaactt cgcttattat ggggatcagt ttcagggttt caagggaagc actcacattg
    13621 tcatcaatct tcgcaacaag gacctcggaa aaatgaataa tcatttcaag tgtattggca
    13681 ttgtgggaca cccacggcac cctactgcac tgacaacaca tgaaatgctc taccgctggc
    13741 tgtgcacaaa aggttacgag gtcatcgttg agcaacaaat cgctcacgaa ctgcaactga
    13801 agaatgtgaa aactggcacg ctcgcggaga ttgggcaact ggctgatctc gcggtagtcg
    13861 ttggtggcga cggtaatatg ctgggcgcgg cgcgcacgct cgcccgttac gatattaaag
    13921 ttattggaat caaccgtggc aacctgggtt tcctgactga ccttgacccc gataacgccc
    13981 agcaacaatt agccgatgtg ctggaaggtc actacatcag cgagaaacgt tttttgctgg
    14041 aagcgcaagt ctgtcagcaa gattgccaaa aacgcatcag caccgcgatt aacgaagtgg
    14101 tacttcatcc tggcaaagtg gcgcatatga tagagttcga agtgtatatc gacgagatct
    14161 ttgcgttttc tcagcgatct gatgggctaa ttatttcgac gccaacaggc tccaccgcct
    14221 attccctctc tgcgggcggt cctattctga ccccctctct ggatgcgatt accctggtgc
    14281 ccatgttccc gcatacgttg tcagcacgac cactggtcat aaacagcagc agcacgatcc
    14341 gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc cagatagcac
    14401 tgccgattca ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat ctgaatctga
    14461 ttcatccgaa agattacagt tatttcaata cattaagcac caagctcggc tggtcaaaaa
    14521 aattattcta attttacgcc agcctcttta ctgtatataa aaccagttta tactgtacac
    14581 aataacagta atggtttttc atacaggaaa acgactatgt tggcacaact gaccatcagc
    14641 aactttgcta tcgttcgtga gcttgagatt gattttcata gcggcatgac cgtaataact
    14701 ggcgagaccg gtgcgggtaa atctattgca atagatgccc tcggtctttg tctcggtggt
    14761 cgcgctgaag ccgacatggt gcgtaccggc gctgctcgcg ctgacctgtg cgcccgtttt
    14821 tctctgaaag atacgccagc ggccctgcgc tggctggaag aaaaccagct tgaagacggg
    14881 catgaatgtt tgcttcgtcg cgtgatcagc agtgatggtc gctcccgtgg tttcatcaac
    14941 ggtacagctg ttcctctgtc acaactgcgc gaactgggtc agttgctgat tcagatccat
    15001 ggtcagcacg ctcatcaatt actcaccaaa cctgagcacc aaaaattcct gcttgatggc
    15061 tatgccaatg aaacctctct acttcaggaa atgaccgcac gttatcagtt gtggcatcaa
    15121 agctgccgtg acctcgcgca tcatcaacag ttaagtcagg aacgcgccgc ccgtgcggaa
    15181 ctgctgcaat accaattaaa agaacttaac gaatttaatc cgcagcccgg agagtttgag
    15241 caaatcgacg aagagtacaa acgtctggcg aacagcggtc aattgctgac caccagccag
    15301 aatgcattgg cattaatggc cgacggtgaa gacgcaaacc tgcaaagtca gctttacacg
    15361 gctaaacaac tggtgagcga attgattggc atggacagca aactgtccgg cgtacttgat
    15421 atgctggaag aagctaccat ccagattgct gaagccagcg acgaactgcg ccactactgc
    15481 gatcgtctgg atctcgatcc caaccgactg tttgaacttg aacagcgcat ctcaaaacag
    15541 atttcgctgg cacgtaaaca tcacgtcagc cctgaggcat taccacagta ttaccagtcg
    15601 ctactggaag aacagcagca actggacgat caggccgact cacaagaaac gctcgcgctg
    15661 gcggtaacga aacatcatca gcaggcactg gaaaccgcgc gcgcattaca ccaacaacgc
    15721 cagcaatatg cagaagaact tgcacagctg atcaccgaca gtatgcatgc gctctcaatg
    15781 ccgcatgggc agtttacgat tgatgtcaaa tatgacgagc atcacctggg cgctgacggt
    15841 gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc agccaatgca gcctattgcc
    15901 aaagtcgcat ccggtggtga attgtcccgc atcgcactgg caatccaggt catcacggcg
    15961 cgtaaaatgg aaaccccggc actgattttt gatgaagtgg atgtagggat tagcggtcca
    16021 acagcggcag ttgtcggcaa actgctgcgt caactcggcg aatcaactca ggtgatgtgt
    16081 gttacccacc tgccacaagt cgcgggatgt ggtcatcaac actattttgt cagcaaagaa
    16141 accgatggtg cgatgacaga aacacatatg caatctctgg ataaaaaagc gcggttacaa
    16201 gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta acacactggc gaatgcgaaa
    16261 gaactgcttg cagcgtaaac ttttttcctg cttcacggtc agagtaaaca gcaaaacgcc
    16321 gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt ctattatcat cggcatatta
    16381 cagatgagcc acgtactgct cgggcccgaa aaggaatcaa atcactatgc gctgtaaaac
    16441 gctgactgct gcagcagcag tactattgat gttaaccgca ggctgttcca ctctggagcg
    16501 agtggtttac cgtcctgaca tcaaccaggg gaactatctg accgctaacg acgtatccaa
    16561 aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac cgctgatgtc
    16621 cgatccattt ggtacgaata cctggttcta tgtcttccgc cagcaaccag gtcatgaagg
    16681 tgtaacgcag caaacgctga cgctgacctt taacagtagc ggtgtgttga ccaatattga
    16741 taacaaacct gcgctgagtg gtaactaata ataaagttgc tctcaaagac gttaaaaaag
    16801 gtgctcaatg agcacctttt ttctgtctgt tatttattcg ctgatttttc tgctcgttgc
    16861 ctgcgaagct ctttcggatc ggcaatgaga gggcgataaa tctccacccg atcgccatca
    16921 tgcacactat cgcttagttt tgccggacgg ctgtaaatgc cgactttgtt tttagttaaa
    16981 tcgatatcgg tacgcaattc cagcaagcca ctggcgcgaa tagcttcttc aaccgtcgcg
    17041 ccctcctgca gcgtcactcg ctgcaggtat tgcttctcag gtagcgcata agccacctca
    17101 acggcaattt tacctagcac tgtaaacctc tttcgcacga accgtaaaag cctggaccat
    17161 attagccgcc agctctttaa acacgcgacc aaaggcgagt tcaatcaact tattggtaaa
    17221 ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg ctcagcggcg taaacttcca
    17281 tccaccaatc aatttcttga acggcccatc caccagattc ataagaatac tttggttact
    17341 ggtcaactgg ttgcgggtag taaacgtttt gctgatccca gccttagaga catctaccgc
    17401 cgcagtcatc tgcccaggag tggactccag aatccgactt ccggtacaac ctggcaaaaa
    17461 ctgaggataa gactgaacgt cattcactaa ctgatacatt tgctccgcgc tgtagggtac
    17521 cagtgcggtc cggctaatct gaggcataac aatttccatc aacaaaaatc caacaaataa
    17581 tatcatttat cctgctgtaa aaaaaacgct atcccggcgc tgggtaacat cgggttcatg
    17641 ctaagataga gccttgtccc ccgcaggatt gatatggggt gttttcgatt tcagattacc
    17701 gatgattcac gacgcttatg acgaagaaaa aagcacataa acctggttca gcgaccatcg
    17761 cgcttaacaa gcgcgcccgt cacgaatact ttatcgaaga agagttcgaa gcgggacttg
    17821 ccctgcaagg ctgggaagtt aaatccctgc gcgcaggaaa agccaatatc agcgacagct
    17881 acgtccttct gcgtgacgga gaggcatttc tgtttggcgc taacatcacg ccaatggccg
    17941 tggcctccac gcatgtggtg tgcgatccta cccgtacccg caagttactt ctcaaccagc
    18001 gcgaactgga ctcattgtac ggtcgcgtca atcgagaagg ctataccgta gtggcgctct
    18061 ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa ggtaaaaaac
    18121 agcacgataa acgttcagat atcaaagaac gcgaatggca ggtggataaa gcacgcatca
    18181 tgaaaaacgc ccaccgttaa acctgcactc caattattga ccagttcctc accgcgcccc
    18241 cctctccggc ggcgcgaatg aacatcttat tggctatcac atccgacaca aatgttgcca
    18301 tcccattgct taatcgaata aaaatcaggc tacatgggtg ctaaatcttt aacgataacg
    18361 ccattgaggc tggtcatggc gctcataaat ctggtatact tacctttaca cattggggct
    18421 gattctggat tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc ggttggcctc
    18481 gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta cgctttagca gcttaataac
    18541 ctgcttagag ccctctctcc ctagcctccg ctcttaggac ggggatcaag agaggtcaaa
    18601 cccaaaagag atcgcgtgga agccctgcct ggggttgaag cgttaaaact taatcaggct
    18661 agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa tgtaaagact gactaagcat
    18721 gtagtaccga ggatgtagga atttcggacg cgggttcaac tcccgccagc tccaccaaaa
    18781 ttctccatcg gtgattacca gagtcatccg atgaagtcct aagagcccgc acggcgcaag
    18841 ccctgcgggc ttttttgtgc tccgaatttg tcccgcgaag tctgatgcca actaattaaa
    18901 tccgatcctt ttaggcacct tgttaggcac ttcataaagc tatattgtct ttgaggtgcc
    18961 taaaactatg gaaacccggc aatggcaaga caaaccaaac ctctatccgt taaggaaatc
    19021 gaatctgcta aacccaagga agcggactac gttctctatg atggcgatgg ccttgagcta
    19081 ctcattaaat ccagcgggag taaaatctgg cagtttcgct acattcgccc tgtcaccaag
    19141 acacgagcaa agaaaagcat aggcccttac ccgtcagtta cccttgccga tgccagaaac
    19201 taccgtgcag agtcccgctc actcctagct aaacagattg atccgcagga acatcagcaa
    19261 gaacaattgc gtagttcgct ggaagccaaa accaatactt tccagctcgt agctgaacgt
    19321 tggtggaatg tgaagaaagc cagtgtgaca gaggactatg cggaagatat ctggcgctct
    19381 cttgaaagag atgtctttcc tgcgattggc gacgttagcg ttacagatat taaagctcat
    19441 acactggttc aggccgtaca accggttcag gccagaggag cactggaaac ggttcgtcgc
    19501 ctgtgtcaac gtattaatga ggtcatgatc tatgcccaga acacagggct gattgatgct
    19561 gttcccagcg ttaatatcgg taaagccttc gaaaagcctc agaaaaagaa catgcccagc
    19621 attcgaccgg atcagctatc tcaactgatg cagacaatgc gaacagccag cattagcctt
    19681 tccacacgct gcctgttcat gtggcaactt cttactatta cccgccctgc cgaagcggct
    19741 gaagctcgct gggaagaagt agacatagaa gcgcaagagt ggaagattcc cgcagcacgc
    19801 atgaaaatga accgcgacca tactgttcca ttgtcagatg aagcaatagc tgtgctggag
    19861 atgatgaaac cgctaagcgg caaccgagaa tttatctttc ctagccgtat caagcccaac
    19921 cagcctatga acagtcagac tgttaacgcc tcactaaaac gcgcaggttt tg
//
LOCUS       DAJVPP010000078        19942 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVPP010000078 DAJVPP010000000
VERSION     DAJVPP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.078"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1291)
                     /gene="eno"
                     /locus_tag="OQM34_002791"
     CDS             complement(<1..1291)
                     /gene="eno"
                     /locus_tag="OQM34_002791"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1453163.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKG"
     gene            complement(1379..3016)
                     /gene="pyrG"
                     /locus_tag="OQM34_002792"
     CDS             complement(1379..3016)
                     /gene="pyrG"
                     /locus_tag="OQM34_002792"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1453164.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3244..4035)
                     /gene="mazG"
                     /locus_tag="OQM34_002793"
     CDS             complement(3244..4035)
                     /gene="mazG"
                     /locus_tag="OQM34_002793"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1453165.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4131..6365)
                     /locus_tag="OQM34_002794"
     CDS             complement(4131..6365)
                     /locus_tag="OQM34_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS1453166.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(6413..7714)
                     /gene="rlmD"
                     /locus_tag="OQM34_002795"
     CDS             complement(6413..7714)
                     /gene="rlmD"
                     /locus_tag="OQM34_002795"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1453167.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            7771..10527
                     /gene="barA"
                     /locus_tag="OQM34_002796"
     CDS             7771..10527
                     /gene="barA"
                     /locus_tag="OQM34_002796"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1453168.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGGIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(10759..12099)
                     /gene="gudD"
                     /locus_tag="OQM34_002797"
     CDS             complement(10759..12099)
                     /gene="gudD"
                     /locus_tag="OQM34_002797"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1453169.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12120..13460)
                     /locus_tag="OQM34_002798"
     CDS             complement(12120..13460)
                     /locus_tag="OQM34_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1453170.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NTGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(13462..14814)
                     /gene="gudP"
                     /locus_tag="OQM34_002799"
     CDS             complement(13462..14814)
                     /gene="gudP"
                     /locus_tag="OQM34_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1453171.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15248..15697)
                     /locus_tag="OQM34_002800"
     CDS             complement(15248..15697)
                     /locus_tag="OQM34_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1453172.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(15715..16497)
                     /gene="truC"
                     /locus_tag="OQM34_002801"
     CDS             complement(15715..16497)
                     /gene="truC"
                     /locus_tag="OQM34_002801"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1453173.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(16497..16826)
                     /locus_tag="OQM34_002802"
     CDS             complement(16497..16826)
                     /locus_tag="OQM34_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1453174.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(17448..17993)
                     /gene="syd"
                     /locus_tag="OQM34_002803"
     CDS             complement(17448..17993)
                     /gene="syd"
                     /locus_tag="OQM34_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1453175.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18061..18909
                     /gene="queF"
                     /locus_tag="OQM34_002804"
     CDS             18061..18909
                     /gene="queF"
                     /locus_tag="OQM34_002804"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1453176.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19021..>19942
                     /gene="ppnN"
                     /locus_tag="OQM34_002805"
                     /gene_synonym="ygdH"
     CDS             19021..>19942
                     /gene="ppnN"
                     /locus_tag="OQM34_002805"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1453177.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTL"
BASE COUNT         4862 a         5367 c         4795 g         4918 t
ORIGIN      
        1 ggcctttgat ctctttacga ccgttgtacg gtgctttttc gcccagagct tcttcgatac
       61 gaatcagctg gttgtattta gcaacacggt cagaacggct catagaacca gttttgatct
      121 ggcctgcagc agtaccaaca gccagatcag cgatggtagc atcttcagtt tcaccagaac
      181 ggtgagagat aactgcagtg tagccagcat ctttcgccat cttgattgca gccagagttt
      241 cggtcagaga accgatctgg ttgaatttga tcaggatgga gttagcgata cctttttcga
      301 taccttcttt caggatcttg gtgttggtta cgaacaggtc gtcaccaacc agctggattt
      361 tgtcgcccag aactttggtc tggtatgcga aaccgtccca gtcagattcg tccagaccgt
      421 cttcgataga aacgatcggg tactgtttgg tcagttcttc caggaagtga gtgaattctt
      481 cagaggtgaa cgctttgttg ccttcgccag ccagaacgta tttaccatct ttgtagaatt
      541 cagaagctgc gcagtccatc gccagagtga tgtctttgcc cagttcataa ccagcagctt
      601 taacagcttc agcgataaca gccagagctt cagcgttgga acccaggttc ggcgcatagc
      661 caccttcgtc accaacagca gtgttcatgc ctttcgcttt cagaactttt gccaggtgat
      721 ggaaaacttc agaacccatg cggatggctt ctttcacagt tttcgcgcca accggctgaa
      781 tcatgaattc ctggatatca acgttgttgt cagcgtgctc accaccgttg atgatgttca
      841 tcatcggaac cggcatggag tatttgcccg gagtgccgtt cagttcagcg atgtgctcgt
      901 acagcggcat acctttagca gctgcagcag ctttggcgtt agccagagat acagccagga
      961 ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc caggtcgatc atgatcttgt
     1021 caatgccagc ctgatcttta gcatctttgc caatcagcgc ctgagcgatc gggccgttta
     1081 ccgcagcaac agctttggtt acgcctttac ccaggaaacg ggatttgtcg ccatcgcgca
     1141 gttccagagc ttcacgggaa ccagtagaag cacctgacgg agcagctgcc ataccgacga
     1201 aaccaccctc cagatgtact tcggcttcaa cagtcgggtt accacgggag tcgatgattt
     1261 cacgaccgat gatttttacg attttggaca ttaggttttc ctcaagtcac tagttaaact
     1321 gaaactccag acaaacaacg cgtacccaag gtacgcgttg ccgttctaac ttttttactt
     1381 acttcgcctg acgtttctgg aactcgctgg cggctttcac aaagcctgca aacagcgggt
     1441 gaccatcacg tggagtagaa gtaaactccg gatggaactg gcaagccacg aaccacgggt
     1501 gattcggaac ttcgatgatc tcgaccaact gatcatcccc ggaacggccc gcaacgcgca
     1561 gacctgcatc ttcaatctgt ttcaacagca tgttgttgac ttcgtaacgg tgacgatgac
     1621 gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag gctatcgtca accaactggc
     1681 actgctgtgc gccgagacgc atggtaccgc cgagatcgct cttctcgcta cgaacttcaa
     1741 cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac aaccgggtac ttacagtctg
     1801 gcacaaattc tgtagagttg gcgttctcca tgttggcaac atggcgagcg taatcaatta
     1861 acgccacctg catacccagg caaatgccca gataaggaat attgttctca cgcgcaaaac
     1921 gcgcggtcgt aatcatgcct tctacgccac gatagccgaa accgccaggt acgaggattg
     1981 cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac atcttgtgaa tcgatcagtt
     2041 tgatgttgac gctgacacga ttcttcagcc caccgtgttt cagtgcttcg atcactgatt
     2101 tataagcatc cggcagttca atgtacttgc cgaccatacc gatggtgact tcacttaccg
     2161 gattcgcttc ttcgaagata acctgttccc attcggacag atttgcttcc gggcagttta
     2221 agctgaatcg tttacaaata taatcgtcca gcccctgaga tttcaacagg cccggaattt
     2281 tatagatgga atcgacgtct ttcagagaaa taaccgcttt ttccggaaca ttacagaaca
     2341 atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc tgaacgacaa atcaggatgt
     2401 caggctggat accgatggag agcagctctt ttacagagtg ctgagtcggt ttggttttga
     2461 cttcaccaga cgctgccatg tacggcacca gcgtcaggtg cataaacaga gtgtgctcac
     2521 ggccaatttc aacagccatc tggcgaatag cttcgaggaa tggcaaggat tcgatatcac
     2581 ctactgtacc gccgatttct accagtacta cgtcatgacc ttcgccacct tccagcacgc
     2641 gctctttgat tgcgttagtg atgtgcggaa taacctgcac ggttgcgccg aggtagtcac
     2701 cgcggcgttc tttacgcaga acgtcagagt agatacgacc cgtggtgaag ttgttgcggc
     2761 ggctcatttt ggtacgaatg aaacgctcgt agtgccccag gtccaagtcg gtttcagcgc
     2821 cgtcttcagt aacgaacact tccccgtgtt ggattgggct catagtacct ggatcgacgt
     2881 tgatgtaagg atccagtttc atgatggtca cattgaggcc acgggcttca agaatggctg
     2941 cgagggaggc tgcggcaatg cctttaccca gagaggatac gaccccgccg gtcacaaaaa
     3001 tatagttcgt tgtcatgctg aacctgagaa gttaggttga aaagacgatg gaataaccaa
     3061 gacgggaaag cagtataccc gaacatgacc tgtgccacaa actttcatta tccctcctct
     3121 tcgccagcgc actattgaaa tcaggagtga gaaaatagcc cctttggggt aaatgttttt
     3181 gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa tggcgcttga ccgcgtaatt
     3241 cccttagaga tcaatttcct gccgttttac ctgttgccag acttcttcca ttgtttcgag
     3301 gtcaacacct gtcatttcca gtccacgcgc ggcaacaata cgctccactt cgcgaaaacg
     3361 acgctcgaat ttttcgttca ctttttgcaa tgcgatttct gctttcgtcc ctaaatggcg
     3421 agccagatta accgtggcaa acagcaggtc ccccatttcc tcctccagtt tagcctggtc
     3481 gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc tcttcgtaga ctttatcaac
     3541 caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg caacgtttct ggattttttg
     3601 cgcccgcatt aaagccggta aactacgagg aatatcgtcc agcgccgaat gctgcgcttt
     3661 ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca agcacttcac tactgttttc
     3721 ggcagagcta tcagcaaaaa catgcggatg gcgacgctct aatttatcgc taatagcagc
     3781 gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc atttgcgcgt aaaacaccac
     3841 ctggaatagc agatcgccca gttcaccgcg aagatcgtca aaatcttcac gggcgatggc
     3901 gtccagcact tcgtaggttt cttcaagggt gtaaggcgca atggtggcaa atgtctgctc
     3961 tttatcccac gggcagccgt tttccggatc gcgcaggcgc tgcataatag tgagcaaacg
     4021 gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc tgtcaggtgt aataccttcg
     4081 agtaaaagat atcgctggat acggctgacg ccatatccag ctatgtaaga ctaactcccg
     4141 tgcaaccgac gcgcgtcgat aacatccggc acctggttga gtttacccag cacgcgcccc
     4201 agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg tcgccagttg ctgtttggtg
     4261 tcgctacggc tggcaacgcc aagcacgttc accttctcgt tggcgagaat ggtcgtgata
     4321 tcacgtaaca acccactacg atcattagcc accacgcgga ccaccagcga atatccggcg
     4381 gagtagctct caccccatac cgcgtcaaca atgcgttctg gcgcatggga gcgcagttcc
     4441 gccagttgtt cgcaatcggc gcggtgtact gaaataccgc gtccctgggt aatgaagccg
     4501 acaatctcat ctccaggaat cggctggcag cagcgcgcga tgtggtgcat caggttacca
     4561 acaccttcaa ccaccacacg accgttatct ttactgcggt tttgcggtgt gtagcttttt
     4621 tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg cactcggctt attaaattgc
     4681 gattgcagga agttcaccat ctgattgaga cggatatccc cgccaccaat cgccgccagc
     4741 aactcgtcga catcattgaa gttgtaacgc ggcagcagat gtttttctgc ttctttcagg
     4801 ctgatcccca gatgttccag ctcgtcgtca aggatttgcc gcccagccag aatgtttttg
     4861 tcacggtcct gtttacggaa ccaggcgtga attttcgaac gcccacggct ggttgtgacg
     4921 taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg gctgtttctg ggtgataatt
     4981 tcaatctggt cgcccatttg cagctggtag gtgaacggca caatgcgccc gccaattttt
     5041 gccccgatgc agcggtgtcc gacatcactg tggatgtggt aagcgaagtc cagcggcgtt
     5101 gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa agacatacac ccggtcgtca
     5161 aacacctgac tacgtacttc gtcgagcatt tcgccggaat cagccatctc ttcctgccac
     5221 gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc ccgaacgtgc gccgccagct
     5281 gccgcgccct ctttatattt ccagtgcgca gcaacaccca actccgcatc ttcatgcatc
     5341 tgtttggtgc ggatttggat ctcaacggtt tttccacccg gccccagaac cacggtatga
     5401 atagactgat aaccgtttgg tttcgggtta gcgacgtaat cgtcaaactc atccggcagg
     5461 tggcgatagt gagtgtgcac tatccccagt gcggcataac aatcctgtaa acgctcggcg
     5521 acaatacgta ccgcacgcac atcaaacagc tcatcgaagg cgaggttctt tttctgcatt
     5581 ttgcgccaga tgctgtagat gtgtttcgga cgaccataca cttcagcttt aacgccttca
     5641 gctttcatct cagcgcgcag atggccgaca aactcttcga tatagtgttc gcggtcgaga
     5701 cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg tcgggtggag ataacggaag
     5761 cagtaatctt ccagctccca tttcagttgc ccaatcccaa gacggttcgc caacggcgcg
     5821 tagatattgg tgcactcttt tgccgccagt acacgttcat cttccggcgc atcttttact
     5881 tcgcgcagat gagcaatacg ctccgccagt ttgatgacta cgcagcgaaa atcatcgacc
     5941 atcgccaata acatccggcg aacgttatcg acctgttcgg aggaaacaga atcagtgtgc
     6001 gtcgctttca gctggcggat cgccgccata tcacgcacgc cgtgaataag gttaacaacc
     6061 gacttaccga cgctctcacg cagcacatct tcgctgacta cgttggcatc agccagaggg
     6121 aacagcagcg ccgcccgcag cgtgtcaatg tccatactta atgtcgagag gatctccacc
     6181 atctcaacac cacgccacaa taacagactg gcatccggat gcccctgcgt ctgttgcaga
     6241 caatacgccc aggtttcggc taagcactca cacgacttct ggctggtaat acccagactt
     6301 gcgatccatt tttccggatc aaattcacca gccttattga tatgtgcact tcttaccgca
     6361 accatcgtcc tctcctttag ggaccagacc tgccgaaatc ggcaaatcgt aactatttaa
     6421 cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg tgggaacata tccagcatcg
     6481 ccagtcgcgc aatggtatat cctgctttta ataacgcttc gctatcccga gccagcgttg
     6541 cagggttaca ggatacataa actatacgaa taggttccag ttttataatt tgctgcataa
     6601 cacctgcggc acctgctcgc gccgggtcca gcaacacttt atcgaagccg tttttcgccc
     6661 acggctgctt tgtgacatct tcttcaagat tttcgtgata aaacgtcaca ttctgtaagc
     6721 cattaagacg cgcattctgc tggccttttt ccaccagcgc aggaacacct tctacaccga
     6781 ccacactggc agcttgtgtc gccaatggca gtgtaaagtt gcccataccg cagaacagat
     6841 ccagtacgcg atcttcaggt tgcacatcca gccattccaa cgcacgcgct accatttttt
     6901 ggttcacacc cgcattgacc tgaataaaat cgcgcgggct aaaagttaag cgcaacccgt
     6961 ttgagtcata ccagggcatc tcaccagaga cggtttcgag tatctcacta tcgggggcga
     7021 gatacagatc caggccttca gaatgcgaaa agcgttccag tttttcgcga tctgccgaac
     7081 ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct ggttgcctgt accagttcaa
     7141 catgaccaag atggcgcata gcttgtaagc tgcccagaca tgccctgact ttgggcagca
     7201 atgcttcaag ttggggcgct aaaatggggc attgtttgac gtcgacaatg tcactggagc
     7261 ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg taagtagttc agacttaaac
     7321 gcgcgcggcg gcgatagccc cagggaacat cggcgatcac ttcagagaca tcgtgtttca
     7381 ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa atccacgctg gcgtgttgtt
     7441 gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg cgtttcgcgt tccgggctat
     7501 cgcttaaccg gcgtacgact ttagcgcggg catactgttt tttatcttca gtaacagcaa
     7561 cttccgcgtt ttcctgcggc aataatccgg ggataaataa cgttttgccg ttatgtcgcg
     7621 ccacgccctg accaaaagag tcgaggtcgt tgactgaaac ggttatgatc tgacgcgtcg
     7681 tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga gactttctca atttaacagt
     7741 gtgaccttaa ttgtcccata acggaactcc atgaccaact acagcctgcg cgcacgcatg
     7801 atgattctga tcctggcacc gaccgtcctt attggtttat tgctgagtat ctttttcgtc
     7861 gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg ccggtgctag cattattgag
     7921 ccgcttgcag tttctactga atatggcatg agcctgcaaa atcgcgaatc tatcggtcag
     7981 ttaataagcg tactgcatcg tcgccattcc gatattgttc gcgcgatttc ggtttatgat
     8041 gaaaataacc gactctttgt cacctccaat tttcatcttg atccctcatc aatgcagctc
     8101 ggcagcaacg tgccgtttcc tcgccagctc actgtcactc gtgacggcgg tattatgatc
     8161 ctccgcacgc cgattatttc tgagagttac tcccccgacg aatcgcccag tagcgatgcc
     8221 aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc tggatcttaa atcggttcgc
     8281 ttgcagcaat ataaagagat ctttatttcc tgcgtgatga tgctgttttg tatcggtatt
     8341 gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg gtccgattcg caacatggtg
     8401 aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc gggtggaagg atttatgctc
     8461 ggcgagctgg atatgctgaa aaacggtatc aactcgatgg caatgtcgct ggctgcttat
     8521 cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg atctgcgtga aacgctggag
     8581 cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa agcgcgccca ggaagcggcg
     8641 cgtattaaat ccgagtttct ggcaaatatg tcacacgagc tgcgtacacc actgaatggt
     8701 gttattggct ttacccgcct gacgctgaaa acagaattaa caccaacgca gcgcgatcac
     8761 ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa ttattaatga tgttctcgac
     8821 ttctcgaaac tggaagcagg taagctgatt ctggaaagta ttccattccc actacgcagc
     8881 acgctggatg aagtcgttac tctgctggca cattcttctc acgataaagg gttggaactg
     8941 acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg gcgacccact gcgattacag
     9001 caaatcatca ctaacctggt ggggaatgca attaaattca ccgagaatgg caacattgat
     9061 attctggtag aaaaacgtgc gctgagtaat accaaagtgc agattgaagt gcagattcgg
     9121 gataccggca ttggtattcc tgaacgcgat caatcgcgct tattccaggc cttccgacag
     9181 gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc tggggctggt gattacacaa
     9241 aaactggtta atgaaatggg cggcgatatt tcgttccata gccagccgaa tcgcggttca
     9301 actttctggt tccacattaa tctcgatctg aacccgaaca ttattatcga agggccatcc
     9361 atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac caaactccgc agcagcgcaa
     9421 tgcacgctgg atattttaag tgaaacgccg ctggaagtgg tttatagccc aacgttctcc
     9481 gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca tcgcggtgac cttccgcgag
     9541 ccgctaacaa tgcaacatga gcgattagcg aaagcggtat cgatgaccga tttcctgatg
     9601 ctggcacttc cttgccatgc acaagttaat gctgaaaaac tcaagcaaga tggtatcggc
     9661 gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc ctgccctgac ggaattttgt
     9721 catcacaaac aaaacacgct tttgcctgta accgatgaaa gtaagctggc aatgacagtc
     9781 atggcggttg atgacaaccc cgctaacctg aaacttatcg gcgcattgct ggaagatatg
     9841 gtgcaacatg tggaactttg cgatagcggg catcaggcag ttgaacgggc gaaacagatg
     9901 ccgttcgatt tgatcttaat ggatattcaa atgccggaca tggatggcat tcgggcctgc
     9961 gaactcatcc accaactccc gcatcagcga caaacgccgg ttatcgcggt aacggcgcat
    10021 gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga tgagcgatta tctggcgaaa
    10081 ccgattgaag aagagcgatt gcataatttg ttgttgcgct acaagcctgg cagcggtatt
    10141 tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg tgaacccgaa tgcgaccctc
    10201 gactggcaac tggcactacg ccaggcagca ggaaaaaccg atttagcgcg cgatatgctg
    10261 caaatgttac tcgatttcct gcctgaagtt cgcaacaaag ttgaggaaca gctggctgga
    10321 gaaaacccgg aaggcctggt tgatttgatt cataaactgc atggcagttg cggctatagc
    10381 ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac aacagctacg tagcggtact
    10441 aaagaagaag atttggagcc ggagctgctg gaactgttgg acgagatgga taatgtcgcg
    10501 cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc gagtagcaat cggcttttta
    10561 tgacgctggt ttcagattgg tgacaaagtg cctgtgttta tgccggatgc ggcgttaacg
    10621 ccttatccgg cctacaaaac caattaaatt caatgaatta aaaaatgatg tagacctgat
    10681 acatagcaca acaggctatt ttgcgtttag catcagtctc aaaccggctc cagatagagc
    10741 cggttttggt tttctgtctt aacgcaccat gcacgggcgc ttgttatcga acgtccagcc
    10801 aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg ccaagcccgt gtttctgata
    10861 cagctcatgg gctttcatca cttgatccat atcgatttct acacccagcc ccggtttttc
    10921 tggcacctgt accagcccgc ctttgatctc aaacggttct ttggtcaggc gctgattacc
    10981 ttcctgccag atccagtgcg tatcaatagc agtaatttta cccggtgcag cggcggcaac
    11041 atgggtaaac atcgccaggg aaatatcgaa gtggttgttt gagtgtgaac cccaggtcag
    11101 gccaaattca tggcacattt gcgccacacg taccgaacct tgcatagtcc agaaatgcgg
    11161 atccgccagc gggatatcaa cggattgtaa cgagagcgta tggcccatct gccgccagtc
    11221 ggtggcgatc atattggttg cagtcggcag gccagtcgcg cgacggaact ctgccatcac
    11281 ttcacgcccg gagaaacctt gctccgcacc acacggatct tctgcataag ccagcgaacc
    11341 tttcaggtat ttaccaattt taattgcttc gttcagcgac caggcaccgt taggatcgag
    11401 cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca atagactcgg cttcttcttc
    11461 cccggccagt acaccgcctt tcagtttgaa atcgttgaag ccatattttt catacgccgc
    11521 ttccgccagg cgcaccaccg catccggcgt catcgcttct tcatgacgca ggcgatacca
    11581 gtcgcatgag tcatccggct ggctttgata cggcagaggt gtggctttgc gattaccgac
    11641 gaagaacaga taaccgagca tttcgacttc gctacgctgt tgaccatcgc ccagcagcga
    11701 tgccacgttt acccccagat gctgccccag cagatccagc attgccgctt ctatcccggt
    11761 aactacatga atagtggtac gcaaatcaaa tgtctgcaaa ccgcgcccac tagcatcacg
    11821 atcggcaaaa gtattacgca ccagcgtcag aacgtttttg tattcaccca gcgttttacc
    11881 taccaccagc ggaatcgcat cttccagcgt tttacggatt ttctcgccgc cgggaatttc
    11941 ccctacgcca gtgtgaccgg aattatcttt gataatcaca atattacgcg tgaagaacgg
    12001 tgcgtgtgca ccactcagat tcatcagcat actgtcatga cccgccaccg ggatgacctg
    12061 catttcagta acaacaggcg tcgtaaattg agaactcata tttatgtcct ttttcagaat
    12121 caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc agccggggat caggtactgc
    12181 atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt tataggcctc atgtgccttt
    12241 tgtacctgtt cccagtccag ttccacgccc agaccgggcg catcaggaac ggcaattttt
    12301 ccgtttttaa tctccagcgg atttttggtc aggcgacaat cgccctcctg ccaaatccag
    12361 tgggtatcga tagcggtagg attacccggt gccgccgcgc ccacatgggt aaatatcgcc
    12421 agagagatat cgaaatggtt gttggaatgg cagccccagg tcagccccca gtcgtcgcaa
    12481 agctgcgcca cgcggactgc accggaaagc gtccagaagt gcggatcggc aagtggaata
    12541 tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc agttggtggc gatcatgtta
    12601 gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca tcacttcacg tccggagaag
    12661 ccctgttctg cgccgcatgg atcttcggca taggtaagaa catcattcag ccctttgcat
    12721 aaagaaatgg cttcatcaag cagccatgca ccgttgggat caacggtaat ccgcgcatcc
    12781 gggaagcttt tcttcaatgc acgaacagtg tcgatttctt gctcgccagg taacaccccg
    12841 cccttaagtt tgaaatcttt aaagccatag cgatcctgtg aggcttccgc cagacgcaca
    12901 acggcttcgc tgttcatcgc tttctgatgg cgcaactgat accactcatg gttgcccggc
    12961 gtattttcca gataaggaag atcggttttg gtccgatcac ctatataaaa cagataaccg
    13021 aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca gttcgcagac cggaacattc
    13081 agcgccttac ctagcaggtc aagcaaggcg gcttccagcg ccgccacggc attaacgcgc
    13141 aattcaaaag tccaggcacc tttgccgaag gtatcaaaat cggctgcctg attaccttta
    13201 tgtacctgct gaaccacttt attcaggcgc gcaacttcct ggcccagaac catcggaata
    13261 gcatcgacca gtgtttgata aatcacctct ccgcccggcg cttcaccaat gccggtatgc
    13321 ccggtgttat cggtgagtac cacgatattg cgggtgaagt atgcgttatg cgcaccacca
    13381 atattgagca acatgctgtc atgtccggcg accggaatga ctttcatatc agtaataaca
    13441 ggactggatt gtgtcgccat cttattgccc cgcgacaggt ttcagctcga tacgcttgat
    13501 atcgcccacc agcaccaggt agctcagtac cgcgattagg gcatgaacac caacataaat
    13561 cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa ccaattgcga ttggcgtgac
    13621 gatgccagaa atgttaccga acatgttgaa caggccaccg gaaagaccgc tgatctcttt
    13681 tggcgcggta tctgccatta ctgcccaacc cagcgccccg atgcccttac cgaagaaggc
    13741 cagcgccata aagccgatga tcatccactc aacgttgacg tagttgcaga acaccatcac
    13801 catcgacagc aacatgccca ttacgatcgg tgttttacgc gcaatgttca gcgatcccgt
    13861 gcggcgcatc agccagtcgg aaataattcc acccagcaca ccgccgataa aaccgcaaac
    13921 cgccggaacg gaagccacaa agcccgcttt cagaatcgac atcccacgcg cctgcaccag
    13981 ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg ttgatacagt actgaccgat
    14041 ataaacgccg atcatcatcc gtgacccgag cagctgtttg atctgccccc acttcacgct
    14101 gaacggcact ttaactttgg tgctttgctg atccatattg atcagcgcgc cacccgcggc
    14161 gatatactcc agctctttct gatttacccc cggatgttga tttggctcgt ggatgacttt
    14221 caaccagata aagctgatga caatgcccag accgcccata aagaagaaga cgtgtgacca
    14281 gcccacttca tgcgtcagcc agcccataat cggcgcgaag atcaccgttg cgaagtattg
    14341 agcggagtta aaaatcgaca ccgccgttcc cctttcctgc accggaaacc aggccgcaac
    14401 aatgcgactg ttgccgggga aagatggcgc ttcagcaagc ccgaccagga agcgcagagt
    14461 aaacagggca acgataatgc cgaatccact aaagatatcg acgaagcctt gcagcaaggt
    14521 aaacatcgac cagataaaga tcgaccagaa gtagacgcgt tttgaaccaa aacgatccag
    14581 caaccaacca ccagggatct gcccgataac ataagcccat gagaaggcag agaacacata
    14641 gcccattccc acgggatcaa ggccgatatc tttggccatt tccgaaccgg caatagaaag
    14701 cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac aacatcacca ctatccagta
    14761 acgagcattc gtgcgttttt ccacactgct cgcagcctga cttaaagaac tcattgttgc
    14821 actcctgaaa attcgcgtta gccacgctca ctctggactg cgacatcgcc aggaaatcag
    14881 aggtgacgta gggtgttttt tgccgttttt ataggtcgtt cgccgaatac ggcgcgtgtt
    14941 tatatctggc aatagcagta taaaaagcgc gccatagcgg ctcaccgtgc aacaacacaa
    15001 cattaatgcg ttcaatgagg cccgattttg gcattagccc tggacggtgg aatccacttc
    15061 acggaaatga aaacaagaac aagaaaggaa gggtaaaaac gaagaaataa agaagagtat
    15121 gaaatggatc gcttgactct aggcaaacgc cagtaaaaat ccgcgctatg aagcagtttt
    15181 tactggcatt tgcctgaaaa gattcgattc agcaccgcta aaacgacatt taccgctcgc
    15241 tgacatatca ggacaacagc gtgccccact gttcgaccca cggatttgat tccgtttccg
    15301 gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc aacccgctga gcgctctgtt
    15361 cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa atttacataa ctgctgtcgc
    15421 cgagcgcaat cacgccataa cgcagattcg gctggaagcc cagactatct ttgattccct
    15481 gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc ggtcgtggac gtaaccacca
    15541 gaacatactt atcctgatag ggcagccagt cgcttaattc aggatcttca aataccgttg
    15601 ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc cgccactaac agcgaatttc
    15661 cgtacatggt gccgacaaaa ataccaattt ccgccatacc gttttctccc tggattagga
    15721 acttatctct ccatcctgac ccgatggcgc actgaactca accctttcat tttcaggaag
    15781 cagaccgcgc cagccaaatt gtgataacgc ctgcatccag gtgtcgtcca ggcccgcgtg
    15841 aatagtcagc ggctcgccag taaaaggatg cgtcaatgac aactgactgg catgtagcat
    15901 taaccgctgg aggccaaaat gctcagcacc gctgcgattc tggcgtaaat cgccatgttt
    15961 gctatcgcca ataatcggat gacgcaaatg ggcaagatgt cggcgaagct gatgtttgcg
    16021 tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg gtcgggtaac gtccggtcgc
    16081 taccggcatt tctacggtcg ccagaccgcg ataatgcgtc actgctggct gcgggccttt
    16141 atcttcgcgg gcaaatttat cagcgatttt gtccagttct tccaccagtg gataatccaa
    16201 caccgcttct tccatcaacc agccgcgcac aatcgcatgg taacgtttct ggatttggtg
    16261 ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg gataatccca tcaacaacac
    16321 accagaagtg ggtcggtcca gacgatgagc agtaaaaaca tgctgtccta tctggtcacg
    16381 cacggtttgc atgaccacta ctttctcgtc gcgatccagc cagctacggt gaaccagcca
    16441 gccggagggt ttatttaccg caaccagcca ttcatcctga tagagtattt ccagcattag
    16501 ctcgcatcat ccgcaaaaag agcatccagt ttttccaact cagccagaat aagcgcgcgt
    16561 tgcggatggt ccgtcgccag cgccatttca taatagggtg caacagcaaa agcgcccggt
    16621 aacggctggt tgttatttaa caaatcgtgc attcgcggga tcagccccca ctgcaaccac
    16681 tccagtggtt ccatggtgtc cataaagaac ggttgggtac tattaaattg atgcggctgg
    16741 ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg cttcaagcgc ctgcaactgg
    16801 agacgaacgc ggtcatgagt ggtcataaag caacctcaat aagaaaaact gccgcgaagg
    16861 atagcaggaa taaaaaaagg gagcactgta ttcacagtgc tcccggttcg tttcgcagca
    16921 ttccagctac ttttgttgct ccctgctcat ccttgacaac ttttcctctg gccttgcggc
    16981 caatcgttca tcctgaacta ttgcttcctg ctcacaccac cccgatgtga tacttcatcc
    17041 tgaagtgtcc ctggccttcc tgacctaccg aatcatcctg accggttctc attctccatc
    17101 ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg cttcatccag aagcctttcc
    17161 ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca tcctgatgtt cacttcgttg
    17221 tctgactccc tgtcgacgaa gatagaatcg tctttttcgc gaagtcttac aaggcgctta
    17281 cagacaatgc cttaaggaaa atttcatatg aaagttaaga attaacaatc aacattttga
    17341 ttttaaatga aaataacgat tcttatcttt ttaagtattc gcttatttcc tggcgatctc
    17401 ctacagagtc tgtaagagat ctctcacaac cagatggggt atttggatta cagaagaggc
    17461 ttaagttgat taaggaattc cgcaagattt gaggctaaat gcgtccgttt acgtgttccc
    17521 agcgtctctt tgcacacttc accggagaga ttacacaccg agatgacctc taactcttct
    17581 tccagcgtcg cgataaatag cgtgggcggc agttttagcc ttttctgagt caccagatga
    17641 ccaatcagat tttcctgcac ccgacggaag tcgtcttcac tccaggtttg cagcaatgtc
    17701 agtttgatat cgccaaactg cgcgtgcata tccccggcaa actgagtcgt ataaaaggta
    17761 tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa ccgcgtttac attttgttcc
    17821 ccggtaaacg gctggggttg ccagtatacg gcatcttcgg tagttgaaat aatgcacggt
    17881 gaaggaacac cgtacaattc ctcacttaac ggccagcttt tatgctcttc atgccacgca
    17941 tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg tcaaatcgtc cactaatttc
    18001 tctcttcact taaaccagat acacttgtcg tttagtttat ctggtttatg acggtgaaac
    18061 atgtcttctt atgcaaacca tcaggcactt gcgggcctga ctcttggaaa atcaaccgat
    18121 taccgggata cctatgacgc cagcctactg caaggcgttc cacgcagcct gaatcgcgac
    18181 ccgctgggtc tgaaagcgga taacctgcct tttcacggta cggatatctg gacgctgtat
    18241 gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg ctgtcggtca tgttgaactt
    18301 gattacacca gcgtaaatct gattgagtcg aagagtttta agctctatct caacagtttt
    18361 aaccagacgc gttttaataa ctgggatgag gtgcgccaga cgctggagcg cgacttaagc
    18421 acttgcgctc agggtaagat tagcgtggcg ttatatcgtc ttgatgaact ggaaggccag
    18481 ccgataggtc atttcaatgg cacttgcatt gatgaccagg atatcactat cgataactat
    18541 gaattcacta ctgactatct ggagaatgcc accagcggtg aaaaagtagt ggaagagacg
    18601 ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc atcaaccaga ttggggttcg
    18661 atccaaattc agtatcgtgg acgccaaatt gacagagaaa aactgctgcg ttacctggtc
    18721 tcattccgtc atcacaacga gttccacgaa cagtgcgtgg aacgcatctt taatgacctg
    18781 ttacgcttct gccagccaga gaaattgagc gtttacgcac gttatacccg tcgtggcggt
    18841 ctggacatta acccgtggcg cagtaatagc gattttgtcc catcgaccac aagactggtt
    18901 cggcaataaa ttttttctca attttgcgtg ctggattcac gcagaaggtt gtgaaaggtc
    18961 atcaggcagg gctattgtaa tcaaagggaa tgacgatatt cgtcccataa ggagtttttc
    19021 ttgattacac atattagccc gcttggctcc atggatatgt tgtcgcagct ggaagtggat
    19081 atgcttaaac gcaccgccag cagcgacctc tatcaactgt ttcgcaactg ttcacttgcc
    19141 gtactgaact ccggtagttt gaccgataac agcaaagaat tgctgtctcg ttttgaaaat
    19201 ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc tggaactgat taatcccccg
    19261 gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc aggccaactt gttcgcggtt
    19321 ctgcgagaca ttctcttcgt ttacgggcaa atccataata ccgttcgttt tcccaacctg
    19381 aatctcgaca actccgtcca catcactaac ctggtctttt ccatcttgcg taacgctcgc
    19441 gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct ggggcggtca ctcaattaac
    19501 gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc tgggcctgcg tgagctgaat
    19561 atctgcaccg gctgtggtcc aggagcgatg gaagcgccga tgaaaggtgc tgcggtcgga
    19621 cacgcgcagc agcgttacaa agacagtcgt tttattggta tgacagagcc gtcgattatc
    19681 gccgctgaac cgcctaaccc gctggtcaac gaattgatca tcatgccgga tatcgaaaaa
    19741 cgtctggaag cgtttgtccg tatcgctcac ggcatcatta tcttccctgg cggtgtgggt
    19801 acggcagaag agttgctgta tttgctggga attttaatga acccggccaa caaagatcag
    19861 gttttaccat tgatcctcac cggcccgaaa gagagcgccg actacttccg cgtactggac
    19921 gagtttgtcg tacatacgct gg
//
LOCUS       DAJVPP010000079        19726 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVPP010000079 DAJVPP010000000
VERSION     DAJVPP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.079"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            91..921
                     /locus_tag="OQM34_002806"
     CDS             91..921
                     /locus_tag="OQM34_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1453178.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPSTAEIHSKMGYNVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1062..1835)
                     /gene="uxuR"
                     /locus_tag="OQM34_002807"
     CDS             complement(1062..1835)
                     /gene="uxuR"
                     /locus_tag="OQM34_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1453179.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2050..3510)
                     /locus_tag="OQM34_002808"
     CDS             complement(2050..3510)
                     /locus_tag="OQM34_002808"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1453180.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3591..4775)
                     /gene="uxuA"
                     /locus_tag="OQM34_002809"
     CDS             complement(3591..4775)
                     /gene="uxuA"
                     /locus_tag="OQM34_002809"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1453181.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5115..6458
                     /gene="gntP"
                     /locus_tag="OQM34_002810"
     CDS             5115..6458
                     /gene="gntP"
                     /locus_tag="OQM34_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCS1453182.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6709..7611)
                     /gene="fimH"
                     /locus_tag="OQM34_002811"
     CDS             complement(6709..7611)
                     /gene="fimH"
                     /locus_tag="OQM34_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCS1453183.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7631..8134)
                     /gene="fimG"
                     /locus_tag="OQM34_002812"
     CDS             complement(7631..8134)
                     /gene="fimG"
                     /locus_tag="OQM34_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCS1453184.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8147..8677)
                     /gene="fimF"
                     /locus_tag="OQM34_002813"
     CDS             complement(8147..8677)
                     /gene="fimF"
                     /locus_tag="OQM34_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCS1453185.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8687..11323)
                     /gene="fimD"
                     /locus_tag="OQM34_002814"
     CDS             complement(8687..11323)
                     /gene="fimD"
                     /locus_tag="OQM34_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCS1453186.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11390..12115)
                     /gene="fimC"
                     /locus_tag="OQM34_002815"
     CDS             complement(11390..12115)
                     /gene="fimC"
                     /locus_tag="OQM34_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1453187.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYSPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12152..12691)
                     /locus_tag="OQM34_002816"
     CDS             complement(12152..12691)
                     /locus_tag="OQM34_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1453188.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12756..13304)
                     /gene="fimA"
                     /locus_tag="OQM34_002817"
     CDS             complement(12756..13304)
                     /gene="fimA"
                     /locus_tag="OQM34_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1453189.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(13785..14381)
                     /gene="fimE"
                     /locus_tag="OQM34_002818"
     CDS             complement(13785..14381)
                     /gene="fimE"
                     /locus_tag="OQM34_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCS1453190.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(14859..15461)
                     /gene="fimB"
                     /locus_tag="OQM34_002819"
                     /pseudo
     CDS             complement(14859..15461)
                     /gene="fimB"
                     /locus_tag="OQM34_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            16915..17631
                     /gene="nanC"
                     /locus_tag="OQM34_002820"
     CDS             16915..17631
                     /gene="nanC"
                     /locus_tag="OQM34_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1453191.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17651..18757
                     /gene="nanM"
                     /locus_tag="OQM34_002821"
     CDS             17651..18757
                     /gene="nanM"
                     /locus_tag="OQM34_002821"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1453192.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            18822..>19726
                     /locus_tag="OQM34_002822"
     CDS             18822..>19726
                     /locus_tag="OQM34_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1453193.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSH"
BASE COUNT         5210 a         4749 c         4512 g         5255 t
ORIGIN      
        1 tgcatgtcat ttgtttactt ctctttttac tacagaaaaa tttatctatt tttctaaaca
       61 tcagttaaat actcataaaa ggtaactatt atgtctatgc cattaagcaa tgccgctaat
      121 aaccaattca atacaaataa tcacttcctt catcatccaa aagtcgacag tgagttaaca
      181 cgtcgatatg aatatgcacg cctggatacg gaaaatatct atcttctgcc tcttgctcgt
      241 ggcaataacc ataattacga tggaaaaagc gttgtcgaaa ttagaaaact taatattggc
      301 aaacagtcct ggccatttaa ttatgtgaca gatacatgca gagaattcaa tggtattaca
      361 acaacaggac gtatgctcta tcgaaacttg aaaatcacct cagctttaga cgaaatatat
      421 ggtggtatat gtaaaaaggc gcatgctgct acagagttag cagaaggttt acggctaaat
      481 ttatttatga agagcccttt cgatcctgtt gaggactata cagttcatga aataacatta
      541 ggccctggtt gcaatgttcc tggttatgcc ggaaccacga taggatatat ttcaaccctt
      601 ccagcttctc aggcaaaaag atggaccaat gagcagccaa gaattgatat ctatattgat
      661 cagataataa ctgtctcagg ggtagcaaat tcttccggat tcgcccttgc cgcattactt
      721 aatgctaata ttgagatggg aaatgaccca atcattggta tagaagccta tcctagtacc
      781 gcggagatcc attcaaaaat gggatataac gttatccccg gtgatgaaaa tgcgccactc
      841 aaacgcatga ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa aaatggtgaa
      901 tggaattata ttggtaaata gccactgaga agaatgagta ctaatattca ggttatacag
      961 atatttaaac aaaaccctgc aatttacaaa gtaatactca ttgcgacttc ttaatttaag
     1021 aaagcgcaag gaacgtttac ccttgcgctt attataataa gtcaggcgtc gactttatcc
     1081 agcggccatg aatcaaagag atagccgtca aaataaatat cgtcaacgtt cgagaattcc
     1141 agcagacgtt gcttaacgtt ttccagatgc tgccacattg ccagcttcgc cgctcgggca
     1201 tcttttttga ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc tttgcgatac
     1261 aggctgtcat ccagatggct gtgcaactgg atccacattg gattgttttc ccgccactgc
     1321 caggactgac ggaataactc caccagcatg ctgttatgcg ttgcttcagc aatggcgaga
     1381 tggaactgca tgtcaccgct ttcgctgcta cccggcgcgc tggaagccag ttcacgctct
     1441 tccagttgca atgcctgacg cattttgacg atatcttcgc gggtagcctg cagtgcggca
     1501 aactcggcga tgttactttc cagtaactga cgcgcctgta acagctcaaa aggacccgca
     1561 tcattgcaga cgttggcatc cggactgtct gtgttgtggc tgcctgagct atcaagcaca
     1621 tagataccgg caccccggcg cacttccacc agccctttga tctccagcat gatcagcgct
     1681 tcacgtacca ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc cggcggcagc
     1741 cgttcgccag gattgtacgg cgtctttatg atcagatcgc ggatcatcgc cccgacttcc
     1801 tggtaaggtc tttgcgcaga ggtgacagat ttcatggtgt tgtgtccgtt aggtgcggtt
     1861 aatcaaattt ttgccggatg cggcgtgaac gccttatccg gcctacaacg ggcaatttta
     1921 cggcccccgt taatctcaaa ctataccatt agcactggaa gttggcacgt acaggtgggc
     1981 aaaaaaacga aaactggtca accaaatatc agtttgattg accagtaagt ctgtagggta
     2041 atcagttagt tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca gctgctgata
     2101 tgccgccgtc accgcgccaa caaagtcggc attctgcggc agatcatcgg caaaaatacc
     2161 gctcaggccc agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt taatcttctg
     2221 gaactcggcc agcatcgggt cgaccacgtc aatggcatta ccctgctcat ccacgccctg
     2281 ggtgtaaccc atccagccag ccacgcccag cgccaggtga cgccagctgc cgccgttttg
     2341 caggtgcaga cgcaccgggt ccagtagacg ctgcggtaac ttctggctgc cgtccatcgc
     2401 aatctgccag gtacggtgac gcagagacgg gttgctgaaa cgctcgatca gcagcgtcgc
     2461 ataggcgttc aggtccgtac cttccggcat cgacagcgtt ggcgcttgtt cctgcatcat
     2521 cagggcaaag gccgctttgc gataatccgg gttagtcatg gtgtcggcaa tggtttcata
     2581 gccgccgagg taaccgaggt acgccaggaa agagtggctg ccgttcagca tacgcagctt
     2641 catcatttcg aacggcacaa cgtctgcaac gaactgtgtg cccactttat cccagtccgg
     2701 gcgaccatta acgaagttat cttcaatcac ccactgacgg aacggttcgc aggcaatggc
     2761 gcacgggtcg taaacaccca gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc
     2821 cggaacgatg cggtcaacca tggtgcacgg gaaggtgacg ttctcttcaa tccatgccgc
     2881 tagctgcggg tcacgcgcct gagccagccc cagtaccgcg accttcgcca catggccgtt
     2941 ttcacgcacg ttatcgcagg acatcaccgt aaatgctttc agcccttttt cacgacgcag
     3001 acgcaaggct tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt tttccagatc
     3061 gtgcttgatc agcgggttat tgagatccag ttgaccgctt gccgcatcag cgcagtagcc
     3121 tttttccgtg accgttagag agacaatcgc cgtttgcgga cgcgccatcg cgttgagaat
     3181 accttcgcag ccgtcgattt ccggatgcag cgcttctttc atcgaaccga taattttcag
     3241 ctcggtgctt tctgcgcctt tttccgctac ggtgtacagc agttgctgtt ttttcaggtt
     3301 ttcgatcagc atgcggtcgt tgcctggcat caggttaact tcgcagatgc cccagtcgct
     3361 gtcggtgctt tccagcagat gatgggtata cagcgcctgg tgcgcgcggt gaaacgcccc
     3421 gcaaccgaga tgcacaatgc gtgattccag acgagaatga tcccatgacg ggcgggcaac
     3481 cggcagattg ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc ttcagagagt
     3541 gacggcaagg aagaaggaac cggcacggcg cgtagccatg ccggtgggta ttaacggcta
     3601 aagaaagcgc gctggatcgc cagttcgaca ccgcgaactt cggccaggcc tttcagacga
     3661 ccaattgcgg agtaaccagg gttggttttc ttcttcaggt cgtccagcat ctgatgaccg
     3721 tggtccggac gcatcgggat caggtcttct ttgccttccg ctttacgacg gtgttcttct
     3781 tcaacaatcg ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg cgccgcttcg
     3841 tggaaggttt tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc
     3901 ggaccgaact gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg
     3961 gtgcacatgg tgaaaccgtt cgccatgctg tttacggtat caaccatcca ctgcatatct
     4021 tcaatggtgg aaacaatgcg cggcaagccg aggatcgggc gcggcggatc gtccgggtga
     4081 accgccatac gcacgccaac ttcttcagca actggaataa tcgctttcag gaagacggca
     4141 aagttttcgc gcagtttggc tttgtcgata tctttgtaca gctccaggtg cttacggaac
     4201 tggtcgaggg tatacccttc ttccgcgccc ggcagaccgg caatgatgtt acgcgtcaga
     4261 cgcgctttgt cttcgtcgct catagtggcg aagcgttcag ctgcctgagc aatttcttct
     4321 tcggtgtaat ccgcttccgc gcctggacgt ttcaggatat gcatttcgaa tgcagcgaat
     4381 tcgatctggt cgaagcgcag agctttggag ccgtctggca gcacgtattc gaggtcagta
     4441 cgggtccagt cgagcaccgg catgaagttg tagcacacgg tgcgaatgcc acactgcgcc
     4501 aggttgcgca gagtctgctg atagttagcg atccactgct catagttgcc agtgtgggtt
     4561 ttgatatctt cgtgaattgg tacgctttct acgacagacc acaccaggcc tgcgtcttca
     4621 acgatcgcct tgcgtttgag gatctcttct acggaccata cttcgccgtt cgggatatgg
     4681 tgcagcgcgg taaccacgcc agttgcgccc gcctgacgga catcagctaa agaaaccgga
     4741 tcgtttgggc cgtaccagcg ccaggtctgt tccatgattt ttcctctttc acatagtggg
     4801 ttccacagca aagagacgcg gaaccttgga gttgtttagg tatccggatg aacaaccaga
     4861 ccgcagccgt ggcgttgcat cggatgagat ggcgtataag ttctaccttt tggtaagaca
     4921 tgtcaaccag aattctgaaa ttggttaacc gcatcgcaag aatttcactc tcatggttgt
     4981 tgctgcaaat tgcagcaaag ggtaatcgat ggttattgat cagaggatgt gacattcatc
     5041 gcaacaatgg ttgaccaatt tacataacat gtcgcccaaa ataacaacgg ttcaaccaag
     5101 taaggtaaaa cgttatgcat gtgcttaaca ttctctgggt ggtattcggc attggcctga
     5161 tgctgatact gaatttgaag ttcaaaatca attcaatggt ggctttgttg gtggcggcgc
     5221 tgtccgtcgg gatgctggca ggcatggatt tgatgtcgct gctgcacacc atgaaagcgg
     5281 gcttcggcaa cacgctggga gaactggcta tcatcgtggt gttcggtgcg gtcatcggta
     5341 aattgatggt cgactccggc gcggctcacc agatagcaca tacgctgctg gcgcgtctcg
     5401 gtctgcgcta tgtacagctg tcggtgatta tcatcggcct tattttcggt ctggcgatgt
     5461 tctatgaagt ggcctttatc atgttagcgc cgctggttat tgttattgcc gccgaagcta
     5521 aaattccgtt cctgaaactg gcgatcccgg cagtagcagc tgccactacc gcacattcac
     5581 tgttcccacc gcagccgggt ccggtggcgc tggtgaatgc ttatggcgcg gatatgggga
     5641 tggtttatat ctatggcgta ctggtgacga tcccaagtgt aatctgcgca ggtctgatcc
     5701 tgccgaagtt cctcggcaat cttgagcgcc caacgccatc attcctgaaa gcagatcaac
     5761 cggtagatat gaataatctg ccctctttcg gcgtttcgat tctggtgccg ctgatcccgg
     5821 cgatcattat gatctctacc accatcgcca atatctggct ggtaaaagat acccctgcct
     5881 gggaagtggt taactttatt ggttcctcgc cgattgcgat gtttattgcg atggtggttg
     5941 cattcgtact ctttggcacc gcgcgtggtc atgacatgca gtgggtgatg aacgcttttg
     6001 aaagcgcggt gaagagtatt gcaatggtga ttctgatcat cggtgcgggt ggcgtgctga
     6061 agcagaccat catcgacacc ggcattggcg acaccatcgg catgttgatg tcccacggca
     6121 atatctcgcc ctacattatg gcatggctga tcactgtgct aattcgtctg gcgacgggtc
     6181 agggtgtcgt ttcggcgatg accgccgccg ggattatcag tgctgcaatc cttgatccag
     6241 caaccggtca gctggttggc gtgaatccgg cgctgctggt actggcgacg gctgcgggtt
     6301 ccaacaccct cacccacatt aatgatgcat ctttctggtt gttcaaaggt tactttgacc
     6361 tgtcagtaaa agacacgttg aaaacctggg gtctgctgga gctggtcaac tccgtggttg
     6421 ggctgattat tgtgctgatt attagcatgg tagcgtaaaa gaacaccaga gcctgccaat
     6481 ggcaggctca gactgatgac taaagtctaa aaatgtgccc ggacagtccc ctcgcccctc
     6541 cggggagagg gttagggtga ggggaacaga ccagtaaagt gcgctttgtt tatgccagat
     6601 gcgacgctga cgcgtcttat ctggcctaca aagggctaac gtgcaggttt tgtaggtctg
     6661 atagcgtagc gcctcaggta ccagcattag caatgcgctg taattttttt attgataaac
     6721 aaaagtcacg ccaataatcg attgcacatt ccctgcagtc acctgccctc cggtacgtgc
     6781 gtaatttgcc gttaatccca gacttaccgc cgaagttcct actgctccta acgataccgt
     6841 gttattcgct ggaataatcg taccgttgcg cgtcaactgt acgccgacgc cctgcgctgg
     6901 tgaaaacgac gcggtattgg tgaaaatcga gttgcccgca tctgcggttg tgccggagag
     6961 gtaatacccc aggttttggc ttttcgcaca ataaacggta agaggaattg gcactgaacc
     7021 agggtagtcc ggcagagtaa cggtgacatc acgagcagaa acatcacaac cgccagtggg
     7081 caccaccaca tcattattgg cgtaaatatt ccacacaaac tgaaaatcat cgctgttata
     7141 gttgttggtc tgtcgcaaaa taagcacggc aattaatgaa ccagctttaa tcgccacccc
     7201 gcccgcactg ctcacaggtg tcaaataaag cgccaccggc cacggcttat ccgttctcga
     7261 attataaaca acccgcggcg tttcgctggt ggtcggaaat ggatagctac tgccactata
     7321 ttttacggtc ccggaaaaat tagataacac gccgccgtaa gccgagcctc gttgcagtgt
     7381 gacatagtct gtaatggttt ccggataatc gttatggcaa aagatttgcg tcgaaagatc
     7441 tacgaccagg ttttgcccca cattcacgac aggcgcaagg tttacataaa cattagcgct
     7501 gccaccgcca atagggatag cggtaccatt ggcggtttta caggcgaatg accaggcatt
     7561 taccgaccag cccatcagca gtacagcaaa cagggtaata gctcgtttca ttacaattat
     7621 ctcttcgggt tcagctgtag gtataggtga tgctaatcac tgcctgaatg gttccctgag
     7681 tggctccgcc atttaccgtc aatgctctga cctgtaacgg gaagtgcgct gattgtgagg
     7741 aatcatccac ctgaactgtt ttggttgcgc cagtattcaa tgtgttgcca ctgtcatcct
     7801 gtagctctaa ctggatgttt tgcgcggtcc cctggttttt ataatatccg gtgctgtcgg
     7861 ctgccccgct gaagctggca gtgaccctcg acgttcccac cggacaatta gtcaactcaa
     7921 gcgcaacatc atgccaggcc gatgccgccc cggcagacat aagactgaaa gaataaagat
     7981 cgccgagatc taccgtggca ttggtggtgg aaaccgtaca cggtttggcg acgaccttac
     8041 cgttcaccgt gatggtgaca tcggctgcct gtatcgtcgc acttgcgagc gccaacattg
     8101 ccgccaatac atacccacgt ttgcaccatt tcatgagcat ctccagttac tgatattcaa
     8161 gagtgaaggt agccgtagca ttgatatgcc ccgcagtgac aggcacctgt gtcgccatta
     8221 gccgggcgta aaaattcagc gtatttggtt tacccggcgt cagggtcgtc cacgaaatcg
     8281 cagacgatgg agcattaagg ggtatttgat tttgctgctc attcagaagc tgtattccca
     8341 gtcccgcagc cgctgacacc gtattttcaa gtgcaagcag gttggcattg tggctatctg
     8401 caacgccggt aaacccaact tttacggcag aaacggcatt accacagggt gacagcaaaa
     8461 tacgaaatgg aacgacagga gtcgtcgcgc caatgttgtt aaattgcttc gccgcgtttt
     8521 ccatcagatc aacagtaaaa ttggttgatt cagcggccac actacagccg ttatctctga
     8581 catagccgcg gatagtaatc gtgctatccg cagccaaaac gcgacttacc gccagccaca
     8641 aaaaagcgca cagaagataa aaaggtttgt ttctcatcac gcccccttaa cgacattcag
     8701 ctgatagctg ggttaataac tgctgctgac tctctggtgg cagttgataa ttggcgacac
     8761 agtgagcatt ttcctcttct ccccatttca cctgaacttt tcccgctaaa ggcattccgc
     8821 tgaggtaaac ctgaccatta tccgcaacaa tgccgctact ctggctactc tctgatgtca
     8881 ccatcgcccc aaacggcagc ggcttattat tgtgagttag cgtcatgagc agttttatcc
     8941 caacgcgcgc tttaaactct gctcgcacga tcgccccacg agtgggaaca acgttagcga
     9001 ccgcgttatc taaatcgacg ttatcagcca aggtattggt atccagcgcc actctatttt
     9061 cccgatattc agtggcataa ggcagcacgg cataaccacg ccagtcggta cgcacccccg
     9121 tctggttttc gacttttgca tcttttgcgc caggcgcttt aacaagcacc accgtatcgt
     9181 ttaacggctg ccccagcgtt acgccattgg catgagccaa taccccaccg ctgactccgt
     9241 aatagagctg cttaatatca tcgctatggc tgtaaccgat attggcattg ccgtaaccac
     9301 cgcgataatt cagcgtggcg tagcctgtac ttccgctatt accatcgcct cccccggcat
     9361 agccggtttg cacgctataa ctgaggttgt tgtcttccag caacgtaccg tatacaccag
     9421 ccagattggt catccgaccg ttgagatcgt gtgacatgct gtagctggca ctggcatgtc
     9481 gccactgaga tttactgtca gaacgcagcc agtggctgaa aggaatattg acgttaagcg
     9541 ctaacatctg atcccgtcct ttttgccagg cgtttttcgt caggctatag ctgagcgtcc
     9601 agttgatatc ttcgaacgca gtatttaatc cagcctggaa ttgctcatcg acattactcg
     9661 ttccccaata agtttgatgg ctaccactca aatacagtgt tgatgtgcgc ccgagttgct
     9721 gagtaacggt gagctgtaat ttcccgcgtt tgttataagc gaggttgtaa tagtcggtga
     9781 atttcggctt aacctgaata actccatcct gtgtttcgat gttgtagcca ttcattcgac
     9841 tgtatgttgt atcagcgaaa ttaaaatatc cgctggtcga ataacggtaa cccactaact
     9901 gaatattcgt gcctgattca ttgagcgatt tgttatagag aaaacgcacc gattgtccgt
     9961 catgctgact gtcatcggga agtgtggaat tagcctgcgt catatccaca gacagagcgc
    10021 ccagtgcctc catgtttttc ccgataccga aattaaaagc acgataacga tccgccagtt
    10081 gcgttccacc atatattgtc cagccagccg gaaggccgtg gagtaatgta ctctggaaaa
    10141 agcggggttt ttcctgctgc gcatttccac tacggtattc tcctgccgta atggaataac
    10201 gagtatgccc ttcacgttgc aaaagcggga ctgacgaata gggtacggta aaaatctgcg
    10261 tgctgccgtc agcctctttg atcgttacct gcaagtcacc actattacct gcggcataga
    10321 tatcgttgat ggtaaaaggc cccggtggca ccgtactatt ataaatgcca tatccatttt
    10381 gtttaatagt gacctgtgca gtaccacgag caataccgtg gatcaccggg gcaaatcctc
    10441 tttggctatc gggtaacata ttgtcatctg aggccaattg tgcgccgcga aagttaatac
    10501 catcgaaaat atcgccctgg gtataaccat cacccaacgt cagccgggaa cgtaaaggaa
    10561 ttatgtctcg ctcaatccag gtaatgatat gctgccattt atttttgcta cctgatgaac
    10621 tgtcgctact gttataactc caggtggtat tgtcgcgtaa acgccacgca ccaatattta
    10681 acccactctg taggtttaaa tatgcataat ggctgttacc cccaatccga ttctgtacac
    10741 tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc cataactcag
    10801 gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc aggttcagtc
    10861 gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt aatggcacac
    10921 aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc cccatactgg
    10981 cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc gtattaaatg
    11041 tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga tacgtccctg
    11101 gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg ggatcatccg
    11161 ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt gcggcaaaag
    11221 cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga atatgcaagc
    11281 attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct ccggtttaaa
    11341 atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt tattccatta
    11401 cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa gtaatattgc
    11461 ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc aatgcatttt
    11521 caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt gtcgggttaa
    11581 tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc tgatcgggtg
    11641 gcaacgctaa tttagccggg ctatagtaca gtttaatgcg gctgataatt gcgagctgta
    11701 gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg ttcatccaga
    11761 ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata cgtaaggtat
    11821 tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga ccatccttta
    11881 caccatcggc attttccacc catgattgaa ttaaataggt actattttca tcattatttg
    11941 tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc gcacctaagg
    12001 ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg atacctgcca
    12061 gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta ctcactgtcc
    12121 tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag aaccaggcct
    12181 gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg gcgatgaaat
    12241 gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga ttaatcggta
    12301 cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc cctggcccct
    12361 ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac gccacaccca
    12421 cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc accggtgcgc
    12481 tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg accgtcattt
    12541 gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga aattgcatat
    12601 ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt ggcagcaacg
    12661 acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa gggtgggcat
    12721 ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac cttgaaggtt
    12781 gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg cgcctggaac
    12841 ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc accgtccagc
    12901 gtcagagcat tgcctgttct gtccaggatc tgcacgccaa cgtttgttgc actacctgca
    12961 gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt acctaagaag
    13021 gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat gttaaaacca
    13081 acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg tcccaactga
    13141 acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac ttccccttta
    13201 aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc ggaactgagg
    13261 gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct ttcctttaaa
    13321 aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg tcaacgagtt
    13381 tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc cattttgact
    13441 catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta atattctatt
    13501 attatcttta tttatagatt tttatattgc atgaggtagt ttttggagag aagaatgagg
    13561 aagatgcgtc gagccacaga aacgttagct ttacatatag cggaggtgat gtgaaattaa
    13621 tttacaagag aaataattta catatcaaat agttagatgc tttttgtcgt tttttaatat
    13681 ttttatgctt gagaaaaaat acgtaactta tttatgatat ggacagtttg gccccaattg
    13741 tcttgtgttt atttgttttt taactttatt atcaataagt taaatcaaac ctcttctctt
    13801 tttaattttt cgtttatgag attatttctt tcccataatc cggcaaaacg agcagcatta
    13861 ctggcggtat aacgcacagt atggcgaata tttcgatgcc cgagataatc ctgaattaaa
    13921 cgggtatctg caccacgctc cgccagttca taaccgcaag catgccttaa catatgagga
    13981 tgagtctgcg ttacggttcc agcttcaata ccggcatcgc gaataatgcg ataggcctgc
    14041 tggcgagaaa gccgactccc gcggcgagaa ataaatatag cgtcggtccg gtcagcgcct
    14101 ttccagttag cacgttcctg ggtccagcgt tccacggctt cacgctcatc aaaacgtaac
    14161 gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattcgacc ttcattaagg
    14221 tcaaggtcct gataatgcag atcaagcagt tcactaatac gcatcccatg ccgatatgcc
    14281 aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc ctgcatcatg
    14341 gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt actcctaact
    14401 gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta gcccaataga
    14461 taaaaagata accacacacg aagttatgtg gctatcagtc aattacatta aacatcatac
    14521 aaaaataaaa tatcactgat gtgacaacat ctattttcat tgatttgatt caatataaca
    14581 atagattaca gaggattaac ttccagttac ttaaaaaata aagaaaaaag tgtggaaaca
    14641 ggagaattta agtgatgtgt aaacttatga atgattttgt ttatttattt tgaagaacat
    14701 aaaccattag attttgtatg ggtatggtta acttaatgtt tattatgata attaagaatc
    14761 acacttacaa taacatcaat taataaatgg ttaatcattt ttgatatatc gtaagaataa
    14821 tgtagttttt aacaccatcc ctggtatctc aactatctct ataaaacagc atgacgctgt
    14881 cgtcctctgg ctctatccca gatgccgtaa aaacgccctg cattgctggc ggtataccag
    14941 acagtatgac gaatattgcg atgcccaaga taatcctaga taagtcgcgt atctattccc
    15001 atattcgcca aagcaaaacc acacgaatag cgtaacatgt gcggatgaat ctccagtgac
    15061 aacccggcat taccaccaga agtcgagata atatggtaaa actgttgccg agaaagcgga
    15121 ttccccttac gtgataaaaa tacccactcg ctctcagcat gcggataaga agtacggata
    15181 ctcaaccagt tttttaaagc ctgaacttct ttgttcaata gcgggtgcgt tgttgaaaag
    15241 ccttttttta atcgatggat atatatacac tttgctttaa gatcaatatc cgaaatcctc
    15301 aatcgacaaa tttcactcgc ccggaaacca tgaataaaac aaagcaaaat cagacaataa
    15361 ttacgtgttg catgaggccc ggtatttgct gctttaagga gtgactcgat ttcactatgg
    15421 gtaaggaagt tccttttttt gttatcagcc ttattcctca tcgttttccc ttataattac
    15481 agacgcgcac tagctgtgct gggttagcaa taatccaaca tttttatcct gattatgttt
    15541 ataaaagcga acgtttgctt aataacttac gtaagtggac cagttcttct gagcgaactt
    15601 aaatggagta gcgatagtta cttataataa ctttgccata caaacgccat gctcttgcat
    15661 gctatgtacc tttatatatt tatcaatctg atacgaatct aataataacc ctcatcatta
    15721 atgcaactaa tcttatctat atatcatgtg atatgttctg taacaagtaa aatcaacata
    15781 aaaaattcag caaaatttaa tatttgttaa actagccgtc tcaaatatga cattattagt
    15841 taactgcatt tgccacaaaa tggctatttc atggaacgca gtaatttctt agtggctatt
    15901 atcatgctaa ttcttagatc cctagcgatt attcctgcct ggttcgtcag cttaggccat
    15961 gagattgtta cattcctttt tatcctggag atatggggga acgcaggcga cgctttttca
    16021 tctacatccg ttggtaaata tagctttggt cactacgact taagccttcc tcaatcacag
    16081 ctattcgccg ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa aacaccaaca
    16141 tcaacaagcc tctccagatc gacatcagga gtgaccagtt acaagccaca aaccaagaaa
    16201 catatttcat gatgagaatt atgctcagcc tgatggcgag taaaccctta ttttcaccac
    16261 tctcactgag ttagcttgca aagctttctt tgtttagatt ctaaataaat tatttataaa
    16321 acatatagtt aaaagaaaat ctttagtttt tgaagaccac cgcaagtgtt cgtctggctt
    16381 cacatggcat cttcttctta gaaaaagatc gacatatttt gtgacgcgaa ttgcaaatct
    16441 ggttttgttg tatggattgc gtgatttttg atctggtata acaggtataa agatgcacca
    16501 cgatagtcaa tgagactggg catctcgtaa tctatggcaa acatcacttc agttctttca
    16561 catcgggtga tgaaaatgca cttcagtctg aaaggaatat gaaaatgaga tcaacagaca
    16621 ctctatttta tgactctggg taaaatgggt tgagtaagtg atataactta cgaatattca
    16681 aatcaattaa aaatcagaag agattttata ctcaggtatt taatctggat ctctgtttat
    16741 ttaaataatg ttaaaagaga attttcacag gagaccttat acaaaaaata taaaatacag
    16801 ttacccgttg ccaaagacac tataagcctg gcaaaaaaat attacacaac ataaatgcta
    16861 attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa atttatgaaa
    16921 aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt aatttctcag
    16981 gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta tgagactcga
    17041 ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag taatacctgg
    17101 aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca ggttgaagtt
    17161 aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt aacgcatttt
    17221 agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc gacaaaagat
    17281 cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca agacttatcc
    17341 ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta ccatattaat
    17401 agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaatga ttatcgctat
    17461 gcaaaccaca agaaatgggc gacggaaaat gcctttgttc tacaatacca tatgacgccc
    17521 gatattacgc catacataga atatgactac cttgaccgcc agggtgttta caacggcaga
    17581 gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta gtagacagga
    17641 gacagtcaca atgaataaaa ctataatggc gcttgctatc atgatggctt catttgccgc
    17701 aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg gagcaattga
    17761 taacgacact gtctacattg gtttaggtag cgcaggtaca gcatggtaca agctagatac
    17821 acaggccaaa gataaaaaat ggactgcgtt agctgcattc cctggtggac cgagagagca
    17881 agcaacctct gcatttattg atggcaatct gtatgtgttt ggcggcattg gcaaaaacag
    17941 cgagggattg actcaggtat ttaatgacgt acacaaatac aaccccaaaa ccaatagctg
    18001 ggttaaattg atgtcgcacg cgccgatggg catggcgggt catgtgactt ttgtacacaa
    18061 cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct attttgaaga
    18121 tctcaacgag gctggaaaag attcaaccgc tatagataaa atcaacgccc actattttga
    18181 caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc cctcaacaca
    18241 gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg cggttgtgaa
    18301 taaaggtgat aaaacctggc ttattaatgg cgaagccaaa ccaggattgc gaacggatgc
    18361 cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg atcccgtctc
    18421 atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc ttatatttgc
    18481 cggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga actatgcgca
    18541 tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg ggaaatggga
    18601 taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct ggaataatag
    18661 tttattaatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt cagttttaat
    18721 ttctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc cggtaaaggg
    18781 gccctgctat caatttgtca tttgtattac gcgaaaatta tatgaacgca ataatatcac
    18841 ccgattatta ctatgttctt accgttgctg gtcagtctaa tgccatggcg tatggcgaag
    18901 gactgccatt accggacagg gaagatgcgc ctcattccag aattaaacaa ttagcgagat
    18961 ttgcgcatac gcatcccgga ggcccgccat gtcactttaa cgacattatt ccactgactc
    19021 actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca acgaatcatc
    19081 aaacacagta cggcaccgtt ggccaggcac tgcatattgc acggaaatta ctgcccttca
    19141 ttcctgataa tgcagggatt cttatcgttc cgtgttgccg tggcggatcg gcttttaccg
    19201 cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct tgtcgttggg
    19261 gaacggatac tccgctgtac caggatttag tcagcagaac gcgagccgca ctggcaaaaa
    19321 atccgcagaa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac ttaatgacca
    19381 gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt cgtagggatc
    19441 taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt tgcggcgata
    19501 ccacctggta ctggaaagaa aatttcccgc attcgtatga agctatttat ggcaattatc
    19561 aaaataatgt tttagccaat attattttcg tcgacttcca gcaacaaggt gaaagaggac
    19621 tgacgaacgc gcctgatgaa gacccggacg atttaagcac gggatattac ggttcagcgt
    19681 accggtcacc ggagaactgg acgacggcac tgcgaagcag tcattt
//
LOCUS       DAJVPP010000080        19616 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVPP010000080 DAJVPP010000000
VERSION     DAJVPP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.080"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..458)
                     /gene="aroD"
                     /locus_tag="OQM34_002823"
     CDS             complement(<1..458)
                     /gene="aroD"
                     /locus_tag="OQM34_002823"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1453194.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAE"
     gene            complement(489..1355)
                     /gene="ydiB"
                     /locus_tag="OQM34_002824"
     CDS             complement(489..1355)
                     /gene="ydiB"
                     /locus_tag="OQM34_002824"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCS1453195.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1367..2638)
                     /locus_tag="OQM34_002825"
     CDS             complement(1367..2638)
                     /locus_tag="OQM34_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1453196.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(2821..4026)
                     /locus_tag="OQM34_002826"
     CDS             complement(2821..4026)
                     /locus_tag="OQM34_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1453197.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAEHFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4126..4482)
                     /locus_tag="OQM34_002827"
     CDS             complement(4126..4482)
                     /locus_tag="OQM34_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCS1453198.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4618..4725)
                     /gene="rprA"
                     /locus_tag="OQM34_002828"
     ncRNA           complement(4618..4725)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQM34_002828"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(4911..6023)
                     /gene="ydiK"
                     /locus_tag="OQM34_002829"
     CDS             complement(4911..6023)
                     /gene="ydiK"
                     /locus_tag="OQM34_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1453199.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6412..9468
                     /locus_tag="OQM34_002830"
     CDS             6412..9468
                     /locus_tag="OQM34_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1453200.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9465..9875
                     /gene="menI"
                     /locus_tag="OQM34_002831"
     CDS             9465..9875
                     /gene="menI"
                     /locus_tag="OQM34_002831"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1453201.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            9975..10163
                     /locus_tag="OQM34_002832"
     CDS             9975..10163
                     /locus_tag="OQM34_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1453202.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10711..11079
                     /gene="sufA"
                     /locus_tag="OQM34_002833"
     CDS             10711..11079
                     /gene="sufA"
                     /locus_tag="OQM34_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1453203.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11088..12575
                     /gene="sufB"
                     /locus_tag="OQM34_002834"
     CDS             11088..12575
                     /gene="sufB"
                     /locus_tag="OQM34_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1453204.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12585..13331
                     /gene="sufC"
                     /locus_tag="OQM34_002835"
     CDS             12585..13331
                     /gene="sufC"
                     /locus_tag="OQM34_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1453205.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13306..14577
                     /gene="sufD"
                     /locus_tag="OQM34_002836"
     CDS             13306..14577
                     /gene="sufD"
                     /locus_tag="OQM34_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1453206.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14574..15794
                     /gene="sufS"
                     /locus_tag="OQM34_002837"
     CDS             14574..15794
                     /gene="sufS"
                     /locus_tag="OQM34_002837"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1453207.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            15807..16223
                     /gene="sufE"
                     /locus_tag="OQM34_002838"
     CDS             15807..16223
                     /gene="sufE"
                     /locus_tag="OQM34_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1453208.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16372..17376
                     /gene="ldtE"
                     /locus_tag="OQM34_002839"
     CDS             16372..17376
                     /gene="ldtE"
                     /locus_tag="OQM34_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1453209.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17439..17675)
                     /gene="lpp"
                     /locus_tag="OQM34_002840"
     CDS             complement(17439..17675)
                     /gene="lpp"
                     /locus_tag="OQM34_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1453210.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(17986..19398)
                     /gene="pykF"
                     /locus_tag="OQM34_002841"
     CDS             complement(17986..19398)
                     /gene="pykF"
                     /locus_tag="OQM34_002841"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1453211.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5119 a         4728 c         5029 g         4740 t
ORIGIN      
        1 tcttcggctt ccggtgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc
       61 gcgtgggcgt aggcgacggt ttctttgacc tgatcatcgc cggtaaataa ctccagatcg
      121 atcatatcaa ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg
      181 gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct
      241 ggcatggttt cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg
      301 gcaaagtggt ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct
      361 tcggatttca cgcgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc
      421 gcaccaatga cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg
      481 tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg
      541 ccagtccata acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc
      601 gttttgcaac cagcttgttg tgcctgctgc aataacttcg tcatatgcgg gttatacacg
      661 cattcagtga ccagcagtcc cggatgtaac agactgatat cattaaccaa tgattcattc
      721 tcaaggggtt tcatacctac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct
      781 tcagcaaagg cttgctgatc ggcgagatcg gtatccgtga cgacacaatc ggtgttttcg
      841 ttaacccgct gcgcgaaggc gagggcttta tcgaagaact catcccgacg gttaaagagt
      901 ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg
      961 gcccctaaca gcaccatcgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga
     1021 atatggcccg tgccgtcggt gttatagcca cgcagatagc catcatcatt aacgatggtg
     1081 ttgatggcac ccaccagttt ggctgctggc gttaattcat caacatattc acacgccagt
     1141 tgtttgttcg gcatcgacac accagttccg cgcattttga gggcttttaa tccttcaatt
     1201 gctccgggaa agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct
     1261 ttttctaagg ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc
     1321 aatccaatca attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct
     1381 taattgtgtt taaccgggtg ctaaatttgc gttcgccaaa ccgcacatca ttctcaggaa
     1441 taataaaaac gcggtaatag cggctaaaaa caataattgc ggtaatcagg gccagcagcg
     1501 cgaaagtaaa atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa
     1561 tcagcggaat aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg
     1621 ctttgctttt ggggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag
     1681 ctgctgagaa accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt
     1741 agataatggc agctgttatt gttgccagcg cagagttaaa tacattagcc cagatgggcc
     1801 ggaccatttt tttcagtagt gcggcaaaaa taaagacaca gaccaacgag cccatactgt
     1861 aataagagat ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt
     1921 tgggcatcca caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg
     1981 atgaaacacc ttccagccag actaaaggtt tgctgttcat ttgcggtaat tcattagcta
     2041 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2101 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2161 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2221 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2281 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2341 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2401 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2461 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag
     2521 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2581 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     2641 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     2701 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     2761 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     2821 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg
     2881 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     2941 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3001 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaatgtt cagccattaa
     3061 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3121 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3181 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3241 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3301 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3361 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3421 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3481 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3541 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3601 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     3661 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     3721 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     3781 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     3841 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     3901 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     3961 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4021 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4081 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4141 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4201 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4261 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4321 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4381 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4441 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4501 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4561 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4621 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     4681 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     4741 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     4801 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     4861 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     4921 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     4981 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5041 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5101 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5161 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5221 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5281 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5341 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5401 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5461 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5521 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5581 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     5641 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     5701 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     5761 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     5821 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     5881 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     5941 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6001 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6061 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6121 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6181 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6241 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6301 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6361 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6421 cagatttccc tggcacccgg cgtcgttcaa ctggtgctta attttttgca agagctggag
     6481 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6541 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6601 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     6661 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     6721 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     6781 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     6841 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     6901 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     6961 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7021 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7081 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7141 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7201 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7261 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7321 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7381 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7441 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7501 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7561 attggctggc aggtgtgccg cgagctggcg ggggttgaac gtatctatgc gatgcgcaaa
     7621 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     7681 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     7741 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     7801 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     7861 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     7921 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     7981 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8041 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8101 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8161 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8221 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8281 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8341 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8401 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8461 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8521 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8581 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     8641 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     8701 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     8761 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     8821 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     8881 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     8941 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9001 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9061 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9121 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9181 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9241 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9301 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9361 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9421 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9481 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9541 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9601 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
     9661 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
     9721 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
     9781 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
     9841 gctgttcgtc acgattgacg actgccattt tgtgacgttg tcaccagaaa agtgtgacgc
     9901 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
     9961 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10021 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10081 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10141 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10201 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10261 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10321 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10381 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10441 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10501 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10561 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10621 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    10681 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    10741 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    10801 ctggtggcaa agcagccagg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    10861 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    10921 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    10981 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11041 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11101 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11161 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11221 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11281 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11341 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11401 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11461 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11521 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11581 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    11641 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    11701 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    11761 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    11821 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    11881 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    11941 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12001 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12061 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12121 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12181 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12241 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12301 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12361 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12421 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12481 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12541 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12601 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    12661 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    12721 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    12781 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    12841 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    12901 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    12961 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13021 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13081 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13141 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13201 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13261 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13321 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13381 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13441 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13501 gcgggagata tatccccaca gcagcgtgat gccttagcgt taacgttaga cgccgtgcgg
    13561 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13621 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    13681 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    13741 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    13801 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    13861 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    13921 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    13981 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14041 ttcctgctgg gtggcgcagt gttacgacac aacaccagta cgcaactcaa tggcgaaaac
    14101 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14161 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14221 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14281 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14341 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14401 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14461 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14521 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14581 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    14641 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    14701 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    14761 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    14821 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    14881 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    14941 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15001 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15061 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15121 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15181 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15241 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15301 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15361 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15421 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15481 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15541 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15601 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    15661 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    15721 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    15781 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    15841 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    15901 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    15961 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16021 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16081 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16141 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16201 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16261 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16321 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16381 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16441 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16501 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16561 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16621 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    16681 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    16741 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    16801 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    16861 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    16921 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    16981 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17041 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17101 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17161 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17221 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17281 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17341 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17401 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17461 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17521 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17581 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    17641 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    17701 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    17761 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    17821 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    17881 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    17941 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18001 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18061 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18121 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18181 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18241 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18301 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18361 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18421 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18481 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18541 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18601 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    18661 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    18721 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    18781 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    18841 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    18901 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    18961 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19021 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19081 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19141 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19201 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19261 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19321 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19381 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19441 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19501 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19561 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattc
//
LOCUS       DAJVPP010000081        19358 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVPP010000081 DAJVPP010000000
VERSION     DAJVPP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.081"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(150..1583)
                     /gene="hldE"
                     /locus_tag="OQM34_002842"
     CDS             complement(150..1583)
                     /gene="hldE"
                     /locus_tag="OQM34_002842"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1453212.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1631..4471)
                     /gene="glnE"
                     /locus_tag="OQM34_002843"
     CDS             complement(1631..4471)
                     /gene="glnE"
                     /locus_tag="OQM34_002843"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1453213.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4494..5795)
                     /gene="ygiF"
                     /locus_tag="OQM34_002844"
     CDS             complement(4494..5795)
                     /gene="ygiF"
                     /locus_tag="OQM34_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1453214.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6037..6657
                     /locus_tag="OQM34_002845"
     CDS             6037..6657
                     /locus_tag="OQM34_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1453215.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6721..7959
                     /gene="cca"
                     /locus_tag="OQM34_002846"
     CDS             6721..7959
                     /gene="cca"
                     /locus_tag="OQM34_002846"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1453216.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8122..8943)
                     /gene="bacA"
                     /locus_tag="OQM34_002847"
     CDS             complement(8122..8943)
                     /gene="bacA"
                     /locus_tag="OQM34_002847"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1453217.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9034..9402)
                     /gene="folB"
                     /locus_tag="OQM34_002848"
     CDS             complement(9034..9402)
                     /gene="folB"
                     /locus_tag="OQM34_002848"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1453218.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGACFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9507..10124
                     /gene="plsY"
                     /locus_tag="OQM34_002849"
     CDS             9507..10124
                     /gene="plsY"
                     /locus_tag="OQM34_002849"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1453219.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10137..11068)
                     /gene="ttdR"
                     /locus_tag="OQM34_002850"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10137..11068)
                     /gene="ttdR"
                     /locus_tag="OQM34_002850"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11275..12118
                     /gene="ttdA"
                     /locus_tag="OQM34_002851"
                     /pseudo
     CDS             11275..12118
                     /gene="ttdA"
                     /locus_tag="OQM34_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12115..12720
                     /gene="ttdB"
                     /locus_tag="OQM34_002852"
     CDS             12115..12720
                     /gene="ttdB"
                     /locus_tag="OQM34_002852"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1453220.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12768..14231
                     /gene="ttdT"
                     /locus_tag="OQM34_002853"
     CDS             12768..14231
                     /gene="ttdT"
                     /locus_tag="OQM34_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1453221.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14274..15287)
                     /gene="tsaD"
                     /locus_tag="OQM34_002854"
     CDS             complement(14274..15287)
                     /gene="tsaD"
                     /locus_tag="OQM34_002854"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1453222.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15525..15740
                     /gene="rpsU"
                     /locus_tag="OQM34_002855"
     CDS             15525..15740
                     /gene="rpsU"
                     /locus_tag="OQM34_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1453223.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15851..17596
                     /gene="dnaG"
                     /locus_tag="OQM34_002856"
     CDS             15851..17596
                     /gene="dnaG"
                     /locus_tag="OQM34_002856"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1453224.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17791..>19358
                     /gene="rpoD"
                     /locus_tag="OQM34_002857"
     CDS             17791..>19358
                     /gene="rpoD"
                     /locus_tag="OQM34_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1453225.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDF"
BASE COUNT         4607 a         5403 c         4936 g         4412 t
ORIGIN      
        1 ttgttactgt taagtttccc gacttactaa caactgatca gaggggggag aaatcctccc
       61 ttacccttgt tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta
      121 cgaagcgaga tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg
      181 atgatgttag tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg
      241 gcccagactt ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt
      301 aaatctggca ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc
      361 cagtcgaccg cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg
      421 cgagaatccc ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcaagcgg
      481 tcacccagtt tgcgggcatt tgccagatag aagacgtgtc cggcgtgcag gatgtcaaag
      541 acgccattgg tcatcaccac tttttcacca cgtttacgcg ctgccgctac ggccagcttc
      601 agttcctctt cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc
      661 agctcgatcg gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca
      721 ttggcaaaga agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg
      781 ccaatcaccg tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata
      841 tgcagcggcg ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac
      901 agagccgaga gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg
      961 gtcttacatt taccgacaac agcttcaaat tccgagagat tcggtgtcag cagcgtagcg
     1021 ccgcggtagc gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta
     1081 cgcgccagtt ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc
     1141 accagcgcgc caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca
     1201 acaccttcga aaccttcttc aaaatccaga cggatcagct gttggttgcg ggaaagtacc
     1261 cgtaatttgg tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg
     1321 tcggccagag atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg
     1381 cgtgcattag caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt
     1441 tcttcgatgg tattcacttt aaccacgggc accggcgctt ccggcgagat acgactggtg
     1501 gggccgtacc agtaacgatc cagcatcaca tcaccaacca ccatcactcc tgcacgttca
     1561 aactctggca gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata
     1621 ataccatact tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct
     1681 ctgcggtgaa gcaatcctcc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa
     1741 gttcatcgcg cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa
     1801 tgtcgttttg cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg
     1861 gtttttcatg agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc
     1921 cgccttcatc ggctttgata tcaaagcgat tacgatgttt attgccgaga tgagcgcgca
     1981 ttttctcgcg catttcccgc acttccgttt gcagagtttt accttctcgc ggcagtgtca
     2041 taatctcacg acgcaccgcg tcaaagtgcg cagtgagctg cggatcgccg tacactacac
     2101 gcgcacgcac cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa
     2161 atgcttctgc ggatgtcacc agcattcccg ccgccccgga cggacgcagt cgagcatcca
     2221 cttcatacaa aatgccggaa gaggtacgcg tactgaacag atgcataatg cgttgcgcca
     2281 gacgcagata aaactgccgc ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg
     2341 ggcaatcatg gaggaagata aggtcaagat cggaactgta gcctaactcc cagccgccca
     2401 gcttgccgta gccgaccacc gcaaaaccgc gcccttcacg ttcgttgagg tgatttggct
     2461 taccgtagcg ggccaccatt tgaccccacg cctgctgaac gacggcatct atcatagctt
     2521 ctgccagcca ggttaagtga tcgctcactt tcattaccgg tagcgtaccg gcgatatccg
     2581 ctgccgcaat gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgtt
     2641 cttcgtcatc ttccggcacg cgcagcaaat actggcgcag ttcatcacgg taggcatcgg
     2701 tcgccgtcgg ctgataaagg gtatttggat cgagcaattc atccagcaat aatggataac
     2761 gcgctagctg gctggcaatc atcggcgatg cggcacacag agaaatcaaa tgtttaagcg
     2821 ccgcagggaa ttcactgagc aattctaaat aggttgtgcg ggtaacaatc cccaccagca
     2881 aggcggtaat gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca
     2941 gatgtggcat cagatggtcg agcacctgac gtcctcgcgg cccgatagta cgcttatcca
     3001 gctctttgcg gaaatcagca atcagcgtta ggacctgttt gcgatcatcc tcgctaagat
     3061 gcgccagcac tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact
     3121 gttccgacag cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca
     3181 cccggcgcac attggtcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa
     3241 agtccatcgc ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtttgggttt
     3301 gttcgtcgtt aatgctttgc agcaggtttt ccagacgccg caggaacaga tatgccactc
     3361 gcaattgttc agcatcgttt tcagaaagca gatgcagcgc ggcaatggcg ctgagcgttg
     3421 gcagtaaaga gcgcgactgc agcaacggtt cgcgcccacc gcgaatgagc tggaacacct
     3481 gaacgataaa ttcaatttcg cgaatgccgc ctgcgccgag tttgatattg tcggtcaaac
     3541 cacgtcgacg cacttcacgg gcaatcatcc ctttcatgtt gcgcagcgac tgaatcacgc
     3601 tgaaatcgat gtaacgacgg aaaacaaacg ggcgcagcat cgcacgcaac tcgttagcat
     3661 agacgccttc gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc
     3721 gtccctgctc ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt
     3781 cgccaaacgg acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat
     3841 ccagcacttt aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc
     3901 ggcgtccgcc ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg
     3961 agaaattcag ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt
     4021 cgccctgcgc attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac
     4081 gcgccgcaac aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag
     4141 taaccagtgc cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc
     4201 gcagctcgcg cattaacccg gcttcgtcgc tcacattact gagcgcctcc tgcaaccaca
     4261 cgctgtaatg ttgccattcg tcagcctgtg gcggttggct ttccagttcc gtcagccact
     4321 ctggatgcgc aatcacgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct
     4381 gtgcactaag tgattcctcg gctaaaagct ctggcagccg ctcaacaacg gtctgccagt
     4441 actgctgtaa cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt
     4501 tttccgctgt gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgttcaatt
     4561 tcaatgcgtt gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggct
     4621 tgcgcgacga caggatcata gtaacccgcc agcaacagta ttgagtcaat atcacgcatc
     4681 aggcgtggca actggtcacg gtagcgatcg cctaacggct ggcagaaaac gcttttcagt
     4741 tcagcggcat ggcgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctaattttg
     4801 ccctgcgctt tggcatctaa aaacggctgc catgctttgc ttaccagcca ttcggtcaac
     4861 gccagcttcg ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa
     4921 gcgatggtcg cttcgcattg agtcagcaaa tcacgtaagt gagtactcgc cttacgcggc
     4981 acaatgccac cgaacagcat cagtgtatga cggactaggc taatggctgc cagcacctgc
     5041 tctttcgccg catcgttgcc gcgtacccac agttcttcat gatactgcca ttgcgctaac
     5101 accagctcga gcgccgcttc cagcccctgt tccacatcgg cttttgccgc aacatgcaaa
     5161 atggtggtcg gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc
     5221 gctttgctca ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc
     5281 agcaccgcgc gcgtgtcgcc gctaagcagc tccagttcca gctcgcagat aggctcagca
     5341 aattcacccg ctttcacttc cccctggtcg agagcgattt caattcggct gccatcgatc
     5401 tctaccagcc atttttcgcg ataaaaatcg gtgctaaaca gcgactgcac gcgggaagcg
     5461 agatcggcag gcaattcgcc gttcggccag atttccgtcg gtaactgcgc caggtcgagc
     5521 gtcggttcgc tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt
     5581 cctgcaactt tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata
     5641 tcgtgcctac gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg
     5701 tcatggtgct cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga
     5761 ttaacaataa actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac
     5821 ataagtgacg atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc
     5881 gccaaattgc catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac
     5941 ctgacacaga ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac
     6001 tatcgttcca ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga
     6061 ttaactttac tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt
     6121 tctgacgaac tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc
     6181 acggttaacg ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc
     6241 caggtgaaag acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag
     6301 ccaagcctgc gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa
     6361 ctcaccaata tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg
     6421 cagagcgaca gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg
     6481 attgtcgcgc agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc
     6541 atcatcatgc aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt
     6601 ctggtactgc cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc
     6661 gccttcttcg ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc
     6721 gtgaagattt atctggtcgg tggtgctgtt cgggatgcat tgttaggact atcggtcaaa
     6781 gacagagatt gggtggtggt cggcagtacg ccacaggaga tgctcgacgc gggctaccag
     6841 caggtaggcc gcgattttcc tgtgtttctg catccgcaaa cgcatgaaga gtatgcgctg
     6901 gcacgtaccg aacggaaatc cggttccggt tacaccggtt ttacttgcta tgccgcaccg
     6961 gatgtcacgc tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag
     7021 gacgataacg gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg
     7081 ttgcgccatg tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt
     7141 tttgctgcgc gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg
     7201 cgcgagatga cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag
     7261 acggaaagcg ccctcaccac ccgcaatccc caggtgttct tccaggtact gcgcgattgc
     7321 ggcgcacttc gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcacctgcc
     7381 aggtggcatc cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg
     7441 atgctgagtc cgcaggttga tgtccgtttc gcaactttat gccacgatct cggtaaagga
     7501 ctgacgccgc cagaactttg gccgcgtcat catgggcatg gcccggcggg tgttaagtta
     7561 gtggaacaac tatgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg
     7621 gtggctgagt ttcacgatct catccgcacc ttcccaatgc tgaacccaaa aaccatcgtc
     7681 aaattatttg attccatcga tgcctggcgt aaaccgcagc gtgtcgagca actggcgctg
     7741 accagcgagg ctgacgtgcg cggcagaacc ggttttgaat cagcggacta cccgcaaggc
     7801 cgttggttgc gcgaaggctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa
     7861 gcgggattta aaggcgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta
     7921 gccagctgga aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttagca
     7981 cgaaccgtcc cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg
     8041 gtgcgaacaa tcacccccac cccggccctc tccctacaga gcgagggaga aaacccggcc
     8101 tgctcccgaa ccttttctca atcaaaagaa cacgacatac accgcagccg ccacaataaa
     8161 gcgataaatg gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat
     8221 cgctatcagc gccaccacaa aagcggtgat aaacccaacg gcaaacatcg ggatatcgcc
     8281 ggttgtcagg aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg
     8341 taccgccagc aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc
     8401 acctgaaatg gtcgccccgg aacgggagaa acccggccac agcgccagac actggaaaca
     8461 gccaatcata aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt
     8521 cggcttcagg cattcggcgg caatcagcaa caaaccgcca acgaccagcg catacatcac
     8581 atttatcggg ttaaacaatg acttaatcgt gtcgtggaat aatagcccca gtaccaccgc
     8641 cggaatcatc cccagcaaaa tgtggatcag cgttaaacga cctttgcttt caccttcgtg
     8701 ctgcaacggg cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac
     8761 tactaccgcc agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc
     8821 ctcaaacccc aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa
     8881 ttctgtcaat ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct
     8941 catcaataaa cccctaaatt attaaaatgt accgcttgtc cgaactactg cagtatgacc
     9001 aggttataac cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc
     9061 acgctcaatg attacgccaa cattcgccgc ccgcgccact gcgcctggct tgctgagttt
     9121 gatacgcacc cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac
     9181 gcgttccacc agcgcaaaac acgccccctc gacgtggctg accaccgttt ctgcaatgtc
     9241 agcgtaactg aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc
     9301 catttcgata tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat
     9361 agtggtgatt accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt
     9421 tggctaaccg gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa
     9481 cacgaatttt caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg
     9541 cgtacctctg cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg
     9601 atccgcgaac cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca
     9661 agggagcagc cgtagcagta ctgattttcg acgttctgaa aggaatgttg cctgtctggg
     9721 gcgcgtatga attaggtgtc agccccttct ggctaggctt aattgccatc gccgcctgtc
     9781 ttggacacat ctggcccgtt ttcttcggtt ttaaaggagg aaaaggcgtt gctaccgctt
     9841 ttggagcaat cgcaccaatt agctgggatc tcaccggagt aatggcggga acctggttac
     9901 tgaccgtgct attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc
     9961 cgttttatgt ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc
    10021 tgatcctgct gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa
    10081 tctggacgaa attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac
    10141 atgacctgat agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat
    10201 tccgcgcaga cacgaatttc atgctgcgat agagcagctc ccggtaaaca gcccagatat
    10261 tggcgctttg tgcatactcc ggcaatactt gcaccagttt gccactctcc agaaacggca
    10321 acacatccca ctcggaacgc agcataatcc ctttgccctc cagcgcccat tgcaacacta
    10381 tctcgccgct attggaggaa agatgcccgc taaccttcac cgattttttc tcctgaccgt
    10441 tccccaactc ccatattccg tgggtcatat cgcgctcttt ggtcaccaga cagtcatgac
    10501 gacttaattc ctgtaatgat tgtggctgcg gatatttttg cagatactca ggtgctgcgc
    10561 ataatattct tttatttttc gttaacagat gcgcaatata ataatcagga atttcgtcat
    10621 taatacgaat atccagatca atattatcct gcactaaatc aatttgccga tcgaacaatt
    10681 caaaatgcac ctgtaactca ggataattgc gcataagttc ggtaatggct ggcgcaatat
    10741 ggctgcgccc aaaaccaaaa ctgcaaccaa tacgaatcat cccctccggg cgcgttttga
    10801 tttgcgtgac gtcatcgacc agtcgctgat attgggtgag gatttccagc gcatgttcgt
    10861 aacaacgttg gccactttca gtcagcgcca cgccgcgagc ggagcggttc agcagcgttg
    10921 ttgccagcgt attttcaagg atttggatcc gcttggtgac aaatgcaggc gtttgcccaa
    10981 gcgtcgccgc tgcagcacta aaactcccgc tgtggacgat ttctaccagt acctgcaagt
    11041 ctttggctaa aggccagcta ttcagcatcg ttaattatcc gcagttgtga taagcgcagt
    11101 gtatttcgca aaacattgcc attccctgct ctggcgcaat attcattctt attttcgcgt
    11161 tgattcacga actgttaatt acatattcac ggtggcaaaa aatataaaac cacattttta
    11221 gtgttagttt gtggcggtga atttttccag acaaatacaa aaactggagt tgccatgatg
    11281 agcgaaagta ataagcaaca ggcagtgaat aagttgacag agattgtcgc taactttacc
    11341 gccatgattt ctacccgaat gcctgatgac gtggtggata aactaaaaca gctaaaggat
    11401 gccgaaacgt cgtcgatggg gaaaattatc taccatacga tgttcgacaa catgcaaaaa
    11461 gcgattgacc tgaatcgtcc tgcctgtcag gacaccgggg agattatgtt cttcgttaaa
    11521 gtcggttccc gcttcccact gcttggcgag ctgcaaagca tactcaaaca agccgtggaa
    11581 gaggcaaccg tcaaagcgcc actacgtcac aatgcggtag aaatttttga cgaagtaaac
    11641 accggcaaaa ataccggtag cggcgtaccg tgggtcacct gggacatcat ccccgacaat
    11701 gacgatgcgg aaatcgaagt ttacatggca ggcggcggct gcacgctacc tggccgctcg
    11761 aaagtgttaa tgccgtcaga aggctacgaa ggcgtggtga aattcgtctt cgaaaatatc
    11821 tccaccctcg ccgtaaacgc ctgtccaccg gtactggtgg gcgtgggcat cgccacctcg
    11881 gtggaaaccg ccgccgtact ctcgcgtaaa gccattttgc gcccgattgg cagccgccat
    11941 cccaatccaa aagcggcaga actggagcta cgcctggaag aaggactcaa ccgtctgggg
    12001 attggtccac aagggctgac cggcaacagt tcagtgatgg gcgcatcgtc gcggcacgct
    12061 gctggttcat gccgatctca cctttgaaaa tctgtctcac acccggagcg cgttatgaaa
    12121 aagatcctga caaccccgat caaagctgaa gatctgcaag atattcgcgt cggcgatgtg
    12181 atctacctga ccggtacgct ggtgacctgc cgcgacgttt gtcaccgccg tttgatcgaa
    12241 ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga ttttccacgc tggccccatc
    12301 gtgcgcaaaa acggtgacaa atgggagatg gtctccgtcg gcccgacaac cagtatgcgt
    12361 atggaaagtt ttgaacgtga atttattgag cagaccggcg taaaactggt ggttggcaaa
    12421 ggtggtatgg ggccgctgac cgaagaaggc tgccagaaat tcaaggcgtt acatgtgatt
    12481 ttcccagcag gctgcgcggt gctggcggca acccaggtag aagagattga agaagtgcac
    12541 tggacagaac tcgggatgcc ggagtcactg tgggtctgcc gggtcaaaga gttcggcccg
    12601 ctgattgtct ctattgatac ccacggcaac aacctgatag ccgaaaacaa aaagctgttc
    12661 gccgaacgcc gcgatcccat cgtggaagag atctgcgagc acgtccatta catcaaataa
    12721 ccctcccgga gaggctcacc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    12781 ctgaatggtg gcgatatctt gcgccgctgg cggtcatcgc cattattgct ctacttccgg
    12841 ttcccgcagg gctggagaac catacctggc tctactttgc cgtttttact ggcgtgatcg
    12901 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atctccatca
    12961 tcgccatact ctctccctgg ctgctgttca gccccgaaca gctcgcccag ccaggcttta
    13021 agttcaccgc caaatccctc tcctgggcag tttctggttt ttctaattcg gttatctggc
    13081 tcattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    13141 ttgcgctgat tctggtgaaa aagatggggc atcgcacgct gtttctcggc tatgcggtga
    13201 tgttctccga gctgatcctg gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggta
    13261 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    13321 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    13381 gcgccatttt cctgacggcg atggcaccta acttgctgtt aattggactg atgaaaagcg
    13441 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagcattt
    13501 tactggttct gctggttccc tggctggctt acgtgctgta cccgccggta ctgaagtctg
    13561 gtgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    13621 gtgaaaaacg gatgctgggg ctgatggtag gcgcgctggt gctgtggatt ttcggcggtg
    13681 attatatcga tgccgcgatg gtcggttaca gcgtggtggc gctgatgctg cttctgcgca
    13741 ttatcagttg ggacgacatt gtcagtaata aagcggcatg gaacgttttc ttctggctgg
    13801 cctcgcttat caccctcgcg accggactca acaataccgg ttttattagc tggtttggca
    13861 aactgttagc aggcagctta agtggttatt cgccgacgat ggtgatggtg acgttgattg
    13921 tggtgtttta tctactgcgc tactttttcg ccagcgccac ggcgtatacc tgcgccctcg
    13981 caccgatgat gatcgccgcg gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    14041 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    14101 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    14161 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    14221 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    14281 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    14341 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaat
    14401 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    14461 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    14521 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    14581 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    14641 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    14701 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    14761 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    14821 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    14881 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    14941 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    15001 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    15061 acagcatcaa tgtcttttgc cgttaaacca gactccttta gcgccgcctg gatcaacggt
    15121 acggttttac gcacatgatc gcgggaggcc agttcaggca cgacgccgcc gtagtcagcg
    15181 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    15241 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    15301 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    15361 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaaggccgcg
    15421 gtgctttaca aagcagcagc aattgcagta aaattccgca ccattttgaa ataagctggc
    15481 gttgatgcca gcggcaaacc gaattaatca aaggtgagag gcacatgccg gtaattaaag
    15541 tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt caagcgttcc tgcgaaaaag
    15601 caggtgttct ggcggaagtt cgtcgtcgtg agttctatga aaaaccgact accgaacgta
    15661 agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa actggctcgc gaaaacgcac
    15721 gccgcactcg tctgtactaa ttccccgaga gcgttgctct ccgatcagac cgagttgtag
    15781 ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt tcgtttatga attgctaaaa
    15841 atcggggcct atggctggac gaatcccacg cgtattcatt aatgatctgc tggcacgcac
    15901 tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa aagcagggca agaatttcca
    15961 cgcgtgttgt ccattccaca acgagaaaac cccgtcattc accgttaacg gtgagaaaca
    16021 gttttaccac tgctttggat gtggcgcgca cggcaacgcg atcgacttcc tgatgaacta
    16081 cgacaagctc gagttcgtcg aaacggtcga agagctggca gcaatgcaca atcttgaagt
    16141 gccatttgaa gcaggcagcg gccccagcca gatcgagcgc catcagaggc aaacgcttta
    16201 tcagttgatg gacggtctga atacgtttta ccaacaatct ttacaacaac ctgttgccac
    16261 gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac gaggttatcg ctcgctttgc
    16321 gattggtttt gcgccccccg gctgggacaa cgtcctgaag cggtttggcg gcaatccaga
    16381 aaatcgccag tcattgattg atgcggggat gttggtcact aacgatcagg gacgcagtta
    16441 cgatcgtttc cgcgaacggg tgatgttccc cattcgcgat aaacgcggtc gggtgattgg
    16501 ttttggcggg cgcgtgctgg gcaacgatac ccccaaatac ctgaactcgc cggaaacaga
    16561 cattttccat aaaggccgcc agctttacgg tctttatgaa gcgcagcagg ataacgctga
    16621 acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg cgcaatacgg
    16681 cattaattac gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca tacaactgtt
    16741 gttccgcgcg accaacaatg tcatttgctg ttatgacggc gaccgtgcag gccgcgatgc
    16801 cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca gacggccgtc agctacgctt
    16861 tatgtttttg ccggatggcg aagaccctga cacgctggta cgaaaagaag gtaaagaagc
    16921 gtttgaagcg cggatggagc aggcgatgcc actctccgca tttctgttta acagtctgat
    16981 gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc tggcactgcc
    17041 attgatatcg caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg aattaggcaa
    17101 caaattaggc atacttgatg acagccagct tgaacgatta atgccaaaag cggcagagag
    17161 cggcgtttct cgccctgttc cgcagctaaa acgcacgacc atgcgtatac ttatagggtt
    17221 gctggtgcaa aatccagaat tagcgacgtt ggtcccgccg cttgagaatc tggatgaaaa
    17281 taagctccct ggacttggct tattcagaga actggtcaac acttgtctct cccagccagg
    17341 tctgaccacc gggcaacttt tagagcacta tcgtggtaca aataatgctg ccacccttga
    17401 aaaactgtcg atgtgggacg atatagcaga taagaatatt gctgagcaaa ccttcaccga
    17461 ctcactcaac catatgtttg attcgctgct tgaactgcgc caggaagagt taatcgctcg
    17521 tgagcgcacg catggtttaa gcaacgaaga acgcctggag ctctggacat taaaccagga
    17581 gctggcgaaa aagtgattta acggcttaag tgccgaagag cgatcgggaa gcccccgaca
    17641 gccgcactga gaggcagcgg caaatatata agtacgccct cgtaattatc gttggcggta
    17701 aacaaccgtt ggatttcagc gttaacggct gaaggacatc gggtcaatcg cccaacacca
    17761 acctcatgaa ataagtgtgg ataccgtctt atggagcaaa acccgcagtc acagctgaaa
    17821 cttcttgtca cccgtggtaa ggagcaaggc tatctgacct atgccgaggt caatgaccat
    17881 ctgccggaag atatcgtcga ttcagatcag atcgaagaca tcatccaaat gatcaacgac
    17941 atgggcattc aggtgatgga agaagcaccg gatgccgatg atctgatgct ggctgaaaac
    18001 accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc tttccagcgt ggaatctgaa
    18061 atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg aaatgggcac cgttgaactg
    18121 ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg aagacgggat caaccaggtt
    18181 caatgctccg ttgctgaata tccggaagcg atcacctatc tgctggaaca gtacgatcgt
    18241 gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg gctttgttga cccgaacgca
    18301 gaagaagatc tggcacctac cgccactcac gtcggttctg agctttccca ggaagatctg
    18361 gacgatgacg aagatgaaga cgaagaagat ggcgatgacg acagcgccga tgatgacaac
    18421 agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac tacgcgctca gtacgttgta
    18481 acgcgtgaca ccatcaaagc gaaaggtcgc agtcacgctg ccgctcagga agagatcctg
    18541 aaactgtctg aagtattcaa acagttccgc ctggtgccga agcagtttga ctacctggtc
    18601 aacagcatgc gcgtcatgat ggaccgcgtt cgtacgcaag aacgtctgat catgaagctc
    18661 tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta ccctgtttac cggcaacgaa
    18721 accagcgata cctggttcaa cgcggcaatt gcgatgaaca agccgtggtc ggaaaaactg
    18781 cacgatgtct ctgaagaagt gcatcgcgcc ctgcaaaaac tgcagcagat tgaagaagaa
    18841 accggcctga ccatcgagca ggttaaagat atcaaccgtc gtatgtccat cggtgaagcg
    18901 aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact tacgtctggt tatttctatc
    18961 gccaagaaat acaccaaccg tggcttgcag ttccttgacc tgattcagga aggtaacatc
    19021 ggcctgatga aagcggttga taaattcgaa taccgccgtg gttacaagtt ctccacctac
    19081 gcaacctggt ggatccgtca ggcgatcacc cgttctatcg cggatcaggc gcgcaccatc
    19141 cgtattccgg tgcatatgat tgagactatc aacaaactca accgtatttc tcgccagatg
    19201 ctgcaagaga tgggccgtga accgacgccg gaagaactgg ctgaacgtat gctgatgccg
    19261 gaagacaaga tccgcaaagt gctgaagatc gccaaagagc caatctccat ggaaacgccg
    19321 atcggtgatg atgaagattc gcatctgggg gatttcat
//
LOCUS       DAJVPP010000082        19148 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVPP010000082 DAJVPP010000000
VERSION     DAJVPP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.082"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..97
                     /locus_tag="OQM34_002858"
     CDS             <1..97
                     /locus_tag="OQM34_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HCS1453226.1"
                     /translation="ITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            388..1116
                     /gene="mpaA"
                     /locus_tag="OQM34_002859"
     CDS             388..1116
                     /gene="mpaA"
                     /locus_tag="OQM34_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1453227.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1091..2056)
                     /gene="ycjG"
                     /locus_tag="OQM34_002860"
     CDS             complement(1091..2056)
                     /gene="ycjG"
                     /locus_tag="OQM34_002860"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS1453228.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            2175..2681
                     /gene="tpx"
                     /locus_tag="OQM34_002861"
     CDS             2175..2681
                     /gene="tpx"
                     /locus_tag="OQM34_002861"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1453229.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2725..4266)
                     /gene="tyrR"
                     /locus_tag="OQM34_002862"
     CDS             complement(2725..4266)
                     /gene="tyrR"
                     /locus_tag="OQM34_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1453230.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4414..5475)
                     /locus_tag="OQM34_002863"
     CDS             complement(4414..5475)
                     /locus_tag="OQM34_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1453231.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5472..6869)
                     /locus_tag="OQM34_002864"
     CDS             complement(5472..6869)
                     /locus_tag="OQM34_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1453232.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7025..8023
                     /locus_tag="OQM34_002865"
     CDS             7025..8023
                     /locus_tag="OQM34_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1453233.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(8134..9006)
                     /gene="ompG"
                     /locus_tag="OQM34_002866"
                     /pseudo
     CDS             complement(8134..9006)
                     /gene="ompG"
                     /locus_tag="OQM34_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(9079..10160)
                     /locus_tag="OQM34_002867"
                     /pseudo
     CDS             complement(9079..10160)
                     /locus_tag="OQM34_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(10174..10833)
                     /gene="pgmB"
                     /locus_tag="OQM34_002868"
     CDS             complement(10174..10833)
                     /gene="pgmB"
                     /locus_tag="OQM34_002868"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCS1453234.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(10830..13097)
                     /gene="ycjT"
                     /locus_tag="OQM34_002869"
     CDS             complement(10830..13097)
                     /gene="ycjT"
                     /locus_tag="OQM34_002869"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCS1453235.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(13094..13717)
                     /locus_tag="OQM34_002870"
     CDS             complement(13094..13717)
                     /locus_tag="OQM34_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1453236.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(13714..14136)
                     /locus_tag="OQM34_002871"
     CDS             complement(13714..14136)
                     /locus_tag="OQM34_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1453237.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(14158..14869)
                     /locus_tag="OQM34_002872"
                     /pseudo
     CDS             complement(14158..14869)
                     /locus_tag="OQM34_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(14887..15939)
                     /locus_tag="OQM34_002873"
     CDS             complement(14887..15939)
                     /locus_tag="OQM34_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCS1453238.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(15970..16812)
                     /locus_tag="OQM34_002874"
     CDS             complement(15970..16812)
                     /locus_tag="OQM34_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCS1453239.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(16799..17680)
                     /locus_tag="OQM34_002875"
     CDS             complement(16799..17680)
                     /locus_tag="OQM34_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCS1453240.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(17701..18984)
                     /locus_tag="OQM34_002876"
                     /pseudo
     CDS             complement(17701..18984)
                     /locus_tag="OQM34_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
BASE COUNT         4620 a         5317 c         4666 g         4545 t
ORIGIN      
        1 aattacagat atcatcgata aacctgagaa acacattggt gagaatatag gcatcaatca
       61 acccgggact gatggcgata agccattctt catgtaaatc aattatatag ataccatcgt
      121 actgtactat gtgcagtacg atttattaaa cttattaagg tgaactgatc aatcagatgc
      181 gattaacatg tgtagaacca gtaagaataa aatacgccat gtatataaca ttacgacaat
      241 tctaatatct gcacatcaat gaaaatgcaa acaacttccg gcaaaaaagt gatacaagat
      301 cgatgacata gcgttgaaga aaagtatttg ataatccgca aaatttgcac aacactgcct
      361 gtaatacttc ctgaaccaga gacgatcatg accgtaaccc gctcacgcgc cgaacgcggc
      421 gcatttccgc ccggaacaga acattacgga cgttcattat tgggcgcgcc gttaatctgg
      481 tttcctgccc ctgcagccag ccgcgaaagt ggtttgattc tggctggcac tcacggtgat
      541 gaaaactctt cggtcgtcac gctctcctgt gctctgcgga ctttgacacc ttctttgcgc
      601 cgccatcatg tggtgctgtg tgtgaatcct gacggctgcc agctggggtt acgggccaat
      661 gctaatggtg tggatttaaa cagaaacttt ccggcggcga actggaagga aggtgaaacg
      721 gtttatcgct ggaacagcgc cgctgaagaa cgtgatgtcg tattactgac aggtgacaaa
      781 cctggctccg aacctgaaac ccaggcgctg tgccagctta tacaccgcat tcaacctgcc
      841 tgggtggtct cattccacga tccactggct tgtattgaag atcccagaca cagcgaatta
      901 ggcgaatggc tggcccagtc gtttgaattg cctctggtaa ccagcgtcgg ttatgaaacg
      961 cctggctctt tcggcagttg gtgtgcagat ctcaacttgc attgcatcac cgcagagttt
     1021 ccgcccatct cctccgacga agccagcgaa aaatatctct ttgcgatggc aaacttgctg
     1081 cgctggcatc ctaaagatgc aattcgcccg tcgtgaactg aagcgccggt tccacatcta
     1141 ccgccagcca ggtcggtccg tcaagatcgg cgaaactgac ctgcggcacc agcggtaaag
     1201 cggcgctaat ggcacgagag gtacacaaca tgcagcccag catcagactg aaaccttgtg
     1261 cacgcgcttc agtcgccagc gccagcgctt ccgtcagacc accggtttta tcgagcttaa
     1321 tgttaaccat ctcatagcgc cctttcagcg ccttcagatt gctacgagta tgacaacttt
     1381 catcagcaca aatcggcaac ggatgaataa aattctccag cgccgcatcg tcctgcgcag
     1441 gaagcggttg ttcaagcatc gcaacgccta aatccgccaa tagctggcaa cgcgccgcca
     1501 acccttctgc acgccaggat tcatttgcat caacgatcag cgtggcatcg ggcacagcgg
     1561 tgcgaattgc caccatccgc tcactgataa gatggttatc cagcttcact ttcagtaatt
     1621 tcgcgcctgc ctgccagagt gttgatgcac tattggccat ctgatcaggc gtaccgatga
     1681 caaccgtctg tgcagtgata actgtctcgg gaagcgttat gccgatcaaa tcagccagcg
     1741 attgctgctg ttttcgcgcc gcgagatccc acaatgcaca atccagcgca ttacgtgctg
     1801 cgccggcagg gagaattttt tgcaactcct cccgtgtcag ccctttctct aattgcggca
     1861 cgacgctcat aatttgcgcc attaccgagg catcactttc tccataacgc ggatacggcg
     1921 tgcattcgcc ggtgccttta ataccctctt cttccagttc aaccaccacc acgcgcgctt
     1981 cactgcgact tccccgggca atcacaaacg gggtatgtaa gggccaggct tcctcaaata
     2041 ccttaacgat tctcatcttt actccttgtt gtcgcggtgg tctgcggatt aagcgcaaat
     2101 agcgtttgct gtgttattga cagttagcat aaactaggtg tgacgttaac tatatgtaaa
     2161 caggaaagat aatcatgtca caaaccgttc atttccaggg caacccggtt acagtcgcca
     2221 attccatccc gcaggcgggt agcaaagcgc agacttttac tctcgtggca aaagatctgt
     2281 ctgacgtcac cctcggtcag tttgcgggta aacgcaaagt gctgaacatt ttcccgagta
     2341 ttgataccgg tgtttgcgcc gcatcagtac gtaagtttaa ccaactggca actgagatcg
     2401 acaacaccgt tgtgctgtgt atctctgccg atctgccgtt cgcccagtct cgtttctgcg
     2461 gcgcagaagg tctgaacaac gttatcaccc tctccacttt ccgtaacgct gaatttctgc
     2521 aagcctacgg tgtggcaatt gctgatggcc cactgaaagg tctggcagcg cgtgccgttg
     2581 tggttattga cgaaaatgac aatgtgattt tcagccagct ggtggatgaa atcaccaccg
     2641 agccggatta cgaagcagct ctggctgtac tgaaagcata agttaatatg cctgatggtg
     2701 ttgcaccatc aggcatattc gcgcttactc ttcgttcttc ttctgactca gaccatattc
     2761 ccgcaactta ttggcaatcg cggtatgtga aacgccgaga cgttttgcca gtttgcgcgt
     2821 gctgggataa ttgcgataaa gctgggttaa taccgagcgt tcaaaacggc tggtgatttc
     2881 gtccagcgaa ccttccatcg catcttcgcc cacggctacc gttgcggcgt cataatccgg
     2941 caacaaaata tcctgtggac gcagctcata accgtccagt tgtgtcagtg cgcgatagat
     3001 agcgttcttt aactgccgca catttcccgg ccacgcataa cgtgtaagta cagtattcag
     3061 gtcagcggcc agtttcggac gcggcacgcc ctgctcgtcg gcaaagcggg cgacgaacag
     3121 ctcagttaac ggcataatgt cctgcggaca gtcacgtagc ggcggcagat tgagcgtcaa
     3181 cacgttcaga cgataataga gatcttcacg gaacacgcct ttttgcacca gttcgaccag
     3241 attcttctgc gtagcgcaaa tcacccgcac atcgacatgc acctcatggt cttcgccaac
     3301 ccgacggaaa gtgccatcat taagaaaacg cagtaatttc gcctgcatcc gtggtgacat
     3361 ttcccctatt tcatccaaca gcaccgaacc accgttcgcc tgctcaaaga accctttctt
     3421 cccttccgga gcatgaccaa acagttcact ctcgaccgca tcttctggta tagacgcgca
     3481 attcagcgcc aggtaaggtt tgcccgctct ggggcttgcc tgatggcagg cgtaggcaaa
     3541 aagatcttta cctgtacctg tgtcacccgt aatcagcagc ggcgcgctta gcatcgccag
     3601 tttctgcgcc tgttcgacaa cgtgcttcat tttcgggctg acggcgacaa tttggctgaa
     3661 ggcgctgacg tcctgggcgg cgacattttg caactggcgg cccatacgaa tcgttgatcg
     3721 caacatcacc accgcaccgg tcaggacgtg ttgatcattt tcatcctgaa gataaacagg
     3781 cgtaatctcc atcaggaaat tctgcccatt aataacgaca tgctcgttat gcgaatcttg
     3841 cggttcgctt tccagccaac gtaaaaaatt aaagccgtta atcaattgtg cggcggtatg
     3901 gttgcgcagg cgatccaatt tttgcccaaa aagctgacag ctcgccgggt tcgccatatc
     3961 cactttgctt ttcatatcga cagagagcac aggttcaggc aacgcctcca gtaacgcgct
     4021 caacgccaga tgctcacgtt cggaaggcat ccacgggaca gtacgcacat cggtaacacc
     4081 tgcaatacgg cgtatttcgg ccatcagact gctgaaactc tcaaactcca gttcagcaaa
     4141 attgaggtag attcgcccaa tgggatcaat ctcaataccg cgtaaatcaa tgcctcttag
     4201 cacgagtaga tcgagtaatt cgcgggtcag accgagtcgg tcttcacaaa agacttccag
     4261 acgcatggga accttcacct gaaaaaagaa caattaatat gatgatatga cactatttgg
     4321 atagcaggaa ggtttctgtc aacaatcatt gacacaaaga cggaaaaacg ggcacttcgg
     4381 cgtaaagata tccggtcagc acccgatatt gcattatttt tcgctgggcg ttttgccttt
     4441 ttgcagagtt tctttcacct gaccgataag ctgacgacgg aaatccccaa ggcgaggttt
     4501 gtcatcgtca atccacggca gcgggcggca aagctccata gctttaatcc cgagtcgtac
     4561 cgtcagaagt ccagcaccaa ttccctgagc tgcgcgggta gacaaacgag cagcgagatc
     4621 ttgcgacatc cagtccatcc ccacttcgcg caccagttcg ctggctccgg caaaagcgat
     4681 attcagcaat accagcttaa acagacgcaa acggctgtaa taccccagtt caatgccata
     4741 cagcgtggca atgcgattaa ttaaacgcag attgcgccag gcgataaacg ccatatcgac
     4801 caacgccagc gggctgaccg caatcatcaa cgttgattcc gccgccgaat ggctgatttc
     4861 gcgccgcgcc tgggcatcta aaactggctg gaccaaatgc gcatacaggc tgaccacttc
     4921 acgatcgttc tgcgtttcat ggattgaggc ataccagcgt tgcagcgccg gatgcgactg
     4981 atcaataccc gcctgctgcg ccagtttttc gcaaaatgcg cgacctttgc ccgtgccgtg
     5041 gctatgcaat aaatcacgcg cttcgtcgcg ttcatgggcg cgctgtcgca agcgccataa
     5101 gcgccgccac tctgttacca cagaacctac gccagcgccg ataatcaatg ccccagcggc
     5161 acatccaccc agcgctaccc agtcctgggt ttgccaggca ttcattgtcc actgtatacc
     5221 ctgcccgaca acgcttgcgc caaacagagc cagcccgccc atcaccattt tgcgccacag
     5281 gctgcgtttc ggacgtaacg ctgcgtccat taccgcttcg acttgcccct cttcttcctg
     5341 cgcttcgtcg agcgtggccg gggcaaaatt ttgcgcctga ttttcgtcaa aggtctgctg
     5401 cgccctgaat tttggattct gttcgacctc cagcggaccg tcgaaatcaa tacgtggttt
     5461 taacggttcg gtcatcgcaa tttatctcct attaaaaatt ccagcgcggc atcaagacga
     5521 atatgcggca gcggtttgtc gacatccatc acctgcgggc gaaacgcttc aaactggaac
     5581 ccttgcttgt cccagaacgc ctgaccgggc aaacgcgcag gaacttcacc ggggtaaacg
     5641 gtgagaggcg caccatcgct gagccggtta ccgcgcaatg ccggaatttt ctcgccgttg
     5701 acgtcaataa tgccgctggt ggtcgcctgc accgaggcca gccccaggca atccatacta
     5761 atcctttcaa acgccgcatt ttgccaggca tcctgaatca gttgttgcag taatgaaacc
     5821 atattggcgt gctgatcgac cgttacatgg tccgctttca tcgcggcaaa cagcagtttg
     5881 tcgataaccg gcgagaataa gcggcgaaac agcgtgcgct gcccgtagtg gaaactttgc
     5941 atcagctgcg tcagtgccag acgcatatca ttaaatgcct gtggcccact gttgagaggt
     6001 tgcaggcaat ccaccagcac aatctggcgg tcaaagcgca gaaaatgatt cttatagaac
     6061 cccttcacca ctttctcgca gtaataatta aaccgctcgc gcagcattcc ggcattggtg
     6121 tgcttatcgg cctgcgccag tttggactcg tcccaggcat cgacatccgg ccacgggaag
     6181 aattgcagcg cgggcgcacc tgccatatct cccggcaaga caaagcgccc aggctgaata
     6241 aagtgcagcc cctgctgttt acagtggtgg agataatcgg tccacacggc ggcaatgtct
     6301 gccagccggt tttcgtcggc aggcgctagc gggtcaagtc cttcgcacat cattcgccat
     6361 ttcgccgacc attcgccgcg ctgaccattg agtaagcccg tcatctggcg cgaccagctt
     6421 aagtaatcct gcgccagcat cggcaggtcg agcaaccatt cgccgggata atccacaatt
     6481 tccagataca gcgtggaggt atccttaaag tggcgcagca gcgaatcgtt cgatttaaaa
     6541 cgtaacgcca ggcgaatttc actgacgccg cgcgtaggcg tcggccaggc gggaggatca
     6601 ccatataact gcgccagtcc ttcatcatag gtgaagcgcg gaatgccaaa gtcacgctgg
     6661 ggaatgcgtt ttacgcccag caagcgctct tcacgcaccg cgcttaacag cggcaaacgt
     6721 gctccggcat gaatattgag caactgatta accatcgcgg tgataaacgc tgttttgccg
     6781 ctgcggctaa gtccggttac agcgaggcgc agatgtctgt cgacaccccg attcaccagc
     6841 gcattgagtt catttttaag tcgcttcatt gctgtcctgt gctgctctga agaccagaaa
     6901 aaaaatcatt cgtaaggata tgaatgtggg ggccaggcca ttgattatca actgcccgtg
     6961 ttgtcatcga cgggcgaact ccataagatg tgcagcattc acgttcggac ggagttcctg
     7021 acctatgtcc cctactattt atgatattgc cagggttgca ggcgtatcaa aatccaccgt
     7081 atcacgcgtg ctgaataagc aaactaatat ctccccggaa gcgcgcgaaa aagtgttacg
     7141 ggccattgaa gaattacagt atcagccaaa caagctggcc cgcgcgctga cctcttcggg
     7201 ttttgatgcc attatggtga tttctacccg ttcgaccaaa actacggcgg gtaatccgtt
     7261 tttctctgaa gttttgcatg ccatcaccgc caaagcggaa gaagaaggtt tcgacgtgat
     7321 attacaaacg tcgcacaatc cggcagaaga tctgcaaaaa tgcgaaagca aaattaaaca
     7381 gaaaatgatt aaaggcatta ttatgctcag ttcgccagcg gatgagtcat tttttgccca
     7441 actcgataaa tacgatattc cggtggtggt aattggcaaa gttgaaggtc aatatgccca
     7501 tgtttattct gtcgataccg ataattttgg tgacagcatt gcgttgaccg atgcattaat
     7561 tgaaagtggg catcaaaata ttgcctgcct gcatgctccg cttgatgtcc atgtttcggt
     7621 tgatcgcgtt aatggctata agcaaagtct gtccgcgcat aatattgcag tgcgtgataa
     7681 atggattgtt gacggcggtt atacccatga aacagcactg caagcagcac ggcaattatt
     7741 aagtcagtca ccgttgcctg aggcagtgtt tgccactgac agcctgaaat taatgagcat
     7801 ttatcgtgcg gcggcagaga aaaatattgc tattccacag cagttagcgg tggtgggtta
     7861 tagtaatgaa acgctgtcgt ttattttaac gcctgcacca ggcggcatcg atgttccgac
     7921 gcaggagtta ggtcaacaaa gctgcgagtt attattccgc ttaatttcgg gaaaaccgtc
     7981 accacagaat attaccgttg ccacgcatat gacgctgaaa taaataccag ctaccttgcc
     8041 tgaatgccgg atgcgatgct ggcgcatctt atccggccta catttataga tcggataaag
     8101 tactcgcgcc gcatccggca atttagccca ttatcagaac gagtaattta cgccgacacc
     8161 tgcataatgg aatttatcgc tgtcgccttc gtcgtgatcc tgccactcaa acgcgtattc
     8221 cagcgaaacg gaaagtccgt tctggaaatc ataaccgtaa aataaaccca cacggttaaa
     8281 atcatggcct tcacgttcaa tatcatcctg ccagtcccag ttactctagc gatccagccc
     8341 aatgcgcgta tacggcgtca ccgagtggtt gccgagcgtc agcggcaaat aggcgcgaat
     8401 ttcttgtgtg gaaaactcac cgttattgcg gctgtcgtcc atattgaagc cgcgctcgag
     8461 ataatagttc actcgcaaag caaccgtttc gttgaaggta tattgcagac ctgtttccgt
     8521 ttcgacacgg gtatcagcgt aaccggtcgt gttcaaatcg ttggcaaatt tatacatcga
     8581 caaccaaccg ttgaaacgta aatcatcagt cagtttcaca tcccagtctg gcgcgatttt
     8641 ccagcgctgc atattcgccg tgtctttgcc cggttcatca acgtagtgat aaccataatt
     8701 acggaaaccg ccggtcaggc cgaaactgaa atcatcgctt tcgaggaact gataatgcac
     8761 ctccagctcc gggcgatcaa acctcgttcc acgtttaccc gcgctataat ctaccggccc
     8821 ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa aatagactga
     8881 aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta tttcgtacat
     8941 cgcaccgata ttaaagtgcc agtcgctctt ttcctcggcc tgtgcgcagg ccattcccgc
     9001 acacatcacc agtgcggtac agggtaataa ctttttttgt tccttgtatt atcctggggg
     9061 aatgccttcc ccctgtattt agcgaattgc tatttccgtt tctgcatcaa agaaatgaca
     9121 tttcgtcata tcaaaatgaa tagtgatatt ttctcctgca tgataatcat ttaacgcacc
     9181 agcacggacc actaactcgt gcccccaacc gtggtgtaga gcataaattc cgcaccggtt
     9241 aattctgcca cgctaatttt ggcggaaatg ttattttcct cttgcgcgtc cggatgaata
     9301 tcttccgggc gtattcccat cacgatgggc ttatgcaaac tttcctgtgt tttcagaacc
     9361 gctaatttct cttcgagaat ggttaattta agcgtttccg taacgaattt atcgccatcg
     9421 atcgtgccgc gaataaaatt catcgctggt gatccaataa atccggcaac aaacatattc
     9481 gcgggttggt tataaacggt tttcggcgca cctacttgct gaacaatccc gtctttcata
     9541 atcacaatcc gcgccgccat ggtcatcgct tcggtctgat cgtgggtcac gtagatcatg
     9601 gtggtgttca gtttctgatg cagcttgctg atctctgcac gcatttgtac gcgcagcttg
     9661 gcatcaaggt tagagagcgg ttcatccatt aaaaacacgc ccgcttcgcg cacaatcgcc
     9721 cgcccgagcg ccacacgctg gcgttgcccg ccggaaagcg cgcccggctt gcgtttcagg
     9781 tactcacgca ggccgagaat ttgcgccgcc cagttcaccc gctcatcaat cacctctttg
     9841 gcgatttttt gcatcttcag gccaaacgcc atgttgtcgt aaaccgtcat atgcggatac
     9901 aacgcgtagt tctggaacac catcgcgata ttgcgtgctt tggctggaac gtcattcatt
     9961 cgtttgccgt cgatcaacag atcgccgccg ctgatctcct caagcccggc aatcatgcgc
    10021 agggtggtcg atttaccgca gcccgacggg ccgacaaata cgatgaactc tttatcggca
    10081 atttccaggt tgaagtcctt caccacatgc acctggttat cgtagatttt ttgaatatgt
    10141 tgtaacgaaa gctgagccat gttgattcct ttgctatacg ttttgccaga aggccgataa
    10201 ccgcgaccag gtcagggagt ccgttgaggg caacagtaat tgcgccccgg ttaagcccgc
    10261 accgatcccc accgagcgca taccgctggc gttaatggcg tcaatgcccg cctgcgcatc
    10321 ttcaatgccg atacatgcct gcggcggcac gcccagcccg gcacaggcag cgagaaagat
    10381 ttccggatcc ggtttcgagt ttttaagttg ggaagcatcc gcgcagaagg tgaaaaactc
    10441 gcgcagctcc agcgccgcta aaatcgtcgg cgcattcagg gagacggaag ccagccctac
    10501 cggaatctgc tgtgcacgga gctctgccag caaattgcga ataccgggca gcacagcatt
    10561 aaccgtcagc tcgcgcagtg agtggacgta aagcagattt ttgcgatacg ccagttgcgc
    10621 ccgctcctgc gggttaaagt cgccctcttt gcccccgtgt tgcagaatgc gccgcagaga
    10681 ctcatcgcgg ctgatccctt ttagagattc gttaaactgc gcatcaatac tgatgccaat
    10741 ttcagcggca atctgctgcc acgcctggaa atgcagatgc gcggtatcgg tgattacacc
    10801 atccagatcg aaaattaccc cttgcagttt cattcatcct cctgatgcgt ggtagcggtc
    10861 ccattgaagg gcaaaataaa atcacccaaa cagaaaacag attcttcagc cacggatata
    10921 agctgaccgt tcaaacgcag tgaaacgggc gcagaagttc gaatcgcaat acgctgcgcg
    10981 tcgagagtga cctgtaattc gcagccctgc cagaacagag ggaaagacaa ctgttgccac
    11041 tgctcaagta acgccggatt tagatgcaat tcaccgtcac gcacgctcac cccggcaaaa
    11101 ccctgaatcg cccccagcca gatagcgcca gttgcggcag catggatacc atcatcacaa
    11161 ctatgcggat cagcaccaag atcgatttca gtcccctcgc gccagaactg ataactttgg
    11221 gtcagcaggc cacagcgtgc agcaacaatg ccgtggattg ctttacttaa tgacgagtcg
    11281 tgaatagtgc gcggttcata aaattgcaga ttggcaagac acgatgccga tgagaactgc
    11341 tctggcagca tgtaattgag catcaccaca tcagcttgtt tgaggatctg catctcgttc
    11401 acttctgcgc gtgaataatc cagcagaatg gtttgcttcc ccgccgccgc tttgtatttc
    11461 gccagattaa tcgccggctt agtcataaac gaatcatcct gcggcaaaac accgtcgggc
    11521 tgaatttctg gcagccgcag ttctttaagg aacatttcgg cacgatgaat aaacgcgtcg
    11581 tcactacagc caaactggcg ggcaatactc agcgcctgct ggacgttgta atacaccata
    11641 tagctggtga aggcgttatt attgacatgt tcggtatatt cgtctggccc aataacatca
    11701 tgaatttcca gacggtcgtt aaccctcacc gcgcggctaa tccagaactt tgcagtttcc
    11761 agaagtagcg ccatgccttc atgcgcaatg aaactttcat cccccgtggt ctgccagtat
    11821 tgaataaccg cccaggcgat atcggccacc agatgatgtt ccgcctgcgc cgaggccact
    11881 ttttgccgca gcccggtgcg aatgttaatg gcggcaaatt ccggcgtctc ttcttcgccg
    11941 ctgcgcgcgc tttcccacgg aaatagcgcg ccctgccagc cgttgcgtcg cgctttctcc
    12001 tgcgcgcctg gcaagttgtg ccagcgataa cgcagtaaac ttcgggcaac cgtcggatcg
    12061 ctaaacagat ggaacggcag caaaaatact tctgtatccc agaaaacgtg gcctttgtag
    12121 ccttcgccgg ttaagccttt tgccgcaata ctgctgcgct cgccgtgagc cggcgtcatg
    12181 atgcgcagat gataaagcgc ataatctaac gcttgctgat cgtgcgcatc gccgccattt
    12241 accgtgatac gacgtttctg ccaccattga cgccagtttt ctgttgatgc tgcaagatgt
    12301 tggtcgtaac tctgttgcgc gcacatttca agctggcgaa gcgacacgct gccccactcg
    12361 tctaaagcag cttgcctgtc atcccgccag tcgatccaca ccagtttttg caacgtcact
    12421 gtctcgcctg catgaagctg cgtactggta tgttgcagta aacggcgctc tttggcggta
    12481 tagcattgct gcacatcacc gctcacctgg cagcaacaac tgatggccac atcactgcgc
    12541 ccatcctggg tggtgtagat cccctgcatc aaatgctgac caaacacccg cacctgggtt
    12601 tcgtcgagat gctggcgacc gtggttggtt tgcgtggcgt caatgcctgt tgaaatcagc
    12661 actgacgcgt cggcgtccag tggcataata gtgatttcca gcgcaatgag cggcagttgg
    12721 tctgccgaaa caaagcgacg gctggcgatg gtgtaacctg cgccgttgct ggtacgccag
    12781 acaacgctac ggcgtaattc tccactggca aagtcaagtt cacgctgcca ggcttcgcgg
    12841 gataacgaga aaacctcacc atttatggca atctccatcc ccaagatatc aggcagattc
    12901 accagttcgt tgatttcacc ttttcccgcc cgatgataca gccccgccag atacatccct
    12961 cgcgtctggc gcgtgtaatc ttcttcatgg ctggcgcgaa gcccaagata gccgttcccc
    13021 tgcgccatca gcgatgcata cttgttcagg gtatgctggc tgaaatgggg ttctgataac
    13081 gttactggcc tggtcataat tccacacgcg tccctgtttc tgcggattga tataacgccg
    13141 ccaccagttg ctggatgata tacccctgct cggcgtcggc tatcatcacg ggcttgccct
    13201 gtacgtgatt gataaaggct tccatgctgc gcaaatggcg gttgtcgtct gccatttccc
    13261 gttgcatcag cgtcattaat tcaccgttgt tatcggtgta gatatgtgct ggaaacagcg
    13321 tcgcaccggc tttatcacca caaaagctga cgttcataat caactgttcg cggatgttga
    13381 gtgcaaatga cgtttccagc cacagaatgc cgccgttatg aaattcaatg gtgccaaaca
    13441 gcgaatcttc gacgctgtaa gttgccggat cccactcgcc aaattgacca cagctctttt
    13501 gcgtgccgat cttttgaaaa ctatgcgcat tcacgctttt caccgccgga aagcccagca
    13561 catacatcgc agcatccagc atatgaatgc cgatgtcgat cagcaggcca ccaccctgca
    13621 gttctttatt ggtaaagaca ccccagccgg gaacgccgca gcgacgcagg gcgcgggcgg
    13681 tggtaacgta aatctctccc aaaacgccgt tggtcacctg ttcacgcagc tgttgcgtat
    13741 cgagcgcaaa cgatggtgaa agtcgtaggc cagcaccttg cccagtttgc gcgcggtatc
    13801 gcacatttcc cacgcctgtt ctgtcgtcat ggcgggcggt ttttcgcaca tcacatggca
    13861 gcctgcttcc agtgccatca gggtatgttc gtaatgaaaa cggttaggtg agccgacgct
    13921 aaccacatca ggtttcaccg ccagcagcat ggcctgcgga tcgtcccaca cggatgcatt
    13981 cccgtatttt tctgccagcg cctgcgcctg ggaaaggcgg ctgtcacaga cagccaccag
    14041 ttccagatcg ttgcgggtgc agtagtacga agcatgaacc ttatccgcca cctggcccgc
    14101 gcctattatc gcgacccgca gcggagagct tgtcattgca cttttcacaa agacctctta
    14161 gcaggtacgc aaccaggcca acgaatcacg gtacgcctgg gcaggatctt ctgcccggat
    14221 acgcccttca tacaccacat aaccctgata gttatcggcg cgcagctgtt caaacagcgc
    14281 gtggaaatcg agggtgccac tgcccggctg gtaacgatgg ttatcggcaa tatgcacatg
    14341 accgagcaag tcacggttgt cgtgcagcgc ctgcgccagg ttatcttctt cgatgttcat
    14401 gtgatagaaa tcgccgataa tctgcacatg tttaagattg ttttcgacga tgtaacgacg
    14461 agcatcggcg agggtgttga tcatatgatc ctgatagcgg tttaacggtt cgagatacac
    14521 cacggttccg gtacgcgcgg cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac
    14581 gctgccggaa cccccccact tctgccagcg cctcgagaat gcgttcgatc tgctttaagc
    14641 cattaagacg acgctcttcg ataaagtcac caatccagcc gtcatagccg ccgcaggcgg
    14701 tggtcactgg taaaccggtt tctttgatcg ccgctttgac ttcttcgatg ttgttgacca
    14761 gcagtttgcc gtcaatctca aaaccatcga agcccatctc tttgatataa cgaaatctct
    14821 cgcggatatt ttccggaaag aatgcctgat tctgtgtgcc gattttcatt ttttcattcc
    14881 tttgaattaa aacgtaacgc ccattttgat gctctgttcc ggatgctgat cgacatactg
    14941 catgtagctt tccgggctgt tggcaaaggt cactaccggg tcgattaaat cttcgcaatt
    15001 gagataaccg ttcatcagca gttcccagca ggtttcttca atacgcttac ggctccagcg
    15061 cggataatcc gggttcggtt cgctgcacgc gcgggagaaa acaattttgg cgttattgaa
    15121 atgcgcttcg cgtccgaggt taaaaccttc agcaaatggt ttggcaaacg cgacatagga
    15181 gatggtgccg ccgtaggcca ggccgcgcag cgccgattgc agcgcgtcgg cgtaaccgct
    15241 ggtttcgata atcacatcag ccccctgctt gccggtcagc gttttgatct ctttgcctac
    15301 gtcagtgcca atggggttaa ggcagaaatc agcaccgtgg cgacgggcaa tatcacagcg
    15361 atgggcgata ggatcgacgc caattaccac cgatgcgcca gcgcgtttcg ccagttggat
    15421 ggcaatttga ccgatcgcgc caagccctac caccacgaca aaatccccta cgcgtacgtt
    15481 ggcatcgcgc acgccgctca tggcaaactg cgccgggtcg taacagacgg cgtttttcca
    15541 ggagctgcct tgcggcattt tgcgcagctt gtagttattc actgcgttaa tgatgaccgt
    15601 ctcggagagc gggccgtagc cgcatacgct gtcgcccacc gcgtagtctg taacgtcgct
    15661 gccgcactcg ataatgtcgc caaccaccat gttgccaagc tggaatttgc caaactcaat
    15721 gccgcgcggc gcatctgccg gacgcggggt gaacatctgc cactcgccgt taaagtcttc
    15781 atcgataaac gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt tcggtgcgcc
    15841 gaaacgcacg cggatcttca cttcattggc taaaatcgcc cgatcttcat actcaaccag
    15901 cgcagcaaca cgcggtgctg tggctactaa ctttttcatg taaaactcct tgtaaagaaa
    15961 ctggccgctt tagcccttca cgccaccggc ggtcaaacca ctcttaataa aacgttccga
    16021 aagcgcatac ataatcacca ccggcaatgc ggtcaccagc gaggccgcca tcatccgtcc
    16081 ccagatgtaa tcaggcgtac tgaacagcgc gttcaggccc actggtaagg tgaaattgct
    16141 ggcgctggag aggaaaatcg acgcaaacag atagtcgttc cacgccacca taaagcagta
    16201 gacgaacacc gatatcagcc cggacatcgc caggggcacg gtgatacgga agatgatttg
    16261 cagacggttg aggccatcca tcatcgccgc ttcttcgatc tcatcaggga tggtgtcgaa
    16321 gtaacttttc agcataaaca cggcggtggg cagggtttgc gtcaccatgg taatgatcag
    16381 cgccatctcg gtgtcgtaaa taccaagcgc ggtgatgatt ttgaacagag gcaccaccag
    16441 caaaatgcca gagaacatat acaccgtgta aaagctggcg ttgatggtca tccggccttt
    16501 aaagcgcagg cgggaaagcg cgtaagctcc gagaatgccg agaaataccg ccaccacgga
    16561 ggaaaccacc gacaccacca tgctgttacg gaagtagtcg acaaacggga aaatcatcgg
    16621 gttaaaaatg tcgacgtaat gctccagcgt ccactgttgc ggcagcagcg taggatgcag
    16681 cgatatcgcc tctttcgcgc ttttgaacga ggtcatcagc atcacaaaaa acggaaacag
    16741 cgtgatgatg agaaacagcg ccagcccgca gtaaaaaccg atacgactga gcgtgcgttt
    16801 atttgttgcc attgaggttc acccgtttcc tggtcagcag aatgacagcg aaaataatga
    16861 tgaagagcac caccgagatc gccgccgctt tccctaagtc attaaaggca aaggcggttt
    16921 tgtagagata gacaccaaga atatcgacct tggttgtcag cagataaaca tcagcgaaca
    16981 tgtagaacat ccagatggta cgcagggtca ccaccgtcgc caggaccggc ataattgcgg
    17041 gcagcgtgac gatgcgaaac cgctgccagg cattagcgcc atccatctct gccgcttcgt
    17101 acagcgattt gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca tacgggaagt
    17161 agcgccagat ggcgaacagc accaccagca caaagctact gcccggatta tcaaaccaca
    17221 gcggtgcctg ctcatagaga tgcagaagat cgacgccgag gtagttcaca atgccgtagc
    17281 cgttgttgaa catgtatttc caggcaaaca ccagcgaaat ggacggcgtt acgtaggaga
    17341 ggatcaccag cgaacgcgcg gttttgcgca ggcggaactc gcggttgaaa aacatcgcca
    17401 cggccagccc gagcacggtg ctgcccgcca ccaccagcgc cgtataccag accgtcatcc
    17461 acagcgagtg ccagaagccg ggatcggaga gaatgcgcac atagttactc accccaacaa
    17521 acgttgactc gatgttggga ttgagcggca gacgtaaaaa actgatttcg atattcgaca
    17581 ccatcggcca cgccaccaga ccgcccagca gtaataagct gggcgcgaga agcagcagcg
    17641 caaacggcat atcggaacga cctgaaaaaa gcctgttcat tgcggattcc tgtcctgggt
    17701 ttagtgctgt tcgatcagtt catccagctt tttctggctc gcttgcagcg tgccagggag
    17761 atcggcttta cccacggtga cgttatgcac catggagctc accacaccag aacccgtcac
    17821 atcgcccatg cgggtaaagt ttttatcccc tactgcgcca aagacctgaa tatttggcag
    17881 ttcaccgatt agctgattcg gtagttcacc cagcgcctta ataacgtcgt tgtctttcca
    17941 ggtggcggta gtgacaaccg ctttattcac cggcagcgca gcacctggcg acatcatcac
    18001 ccagtcggca atgttgtccg cctgctccat aaaggtgaca aatttttctg ctgcttcggt
    18061 cttttgtccg gcggtaatgg tcagcgaggt caacatgccg tagaccgcag agtttttctc
    18121 ggttggcacc acgaaaccga cgtttttcgg gtcgccttct ttaatcacag ccggaaggat
    18181 ataggtggag taaatcgcca tcggcgcggt gccgttcata aaggcgtctt tcacttccat
    18241 gatgtcgtta gaacccggca tggtgttggc agcaaggtcg cggtaatagg tcagcgcctg
    18301 catcatctct ggtgtatcaa gggtgatttt gccttcggcg ttaaagacgt tggcctagtt
    18361 ggataacgca aactgggaga aggattgttc cgtcagcacg ctttctgccg taggcagcgc
    18421 aataccgtat tttttattcg ccgggtcatt cagtttctgt gcaacgtcca gcagctgttg
    18481 ccagtttttc ggctcctcca gccccgcttt tgccagcaca tctttgcgat accagatgcc
    18541 gccaatccag gcgctgacag gaacaccggt ccatgcgcta ccatcttcgg tacgcacaat
    18601 acgcagtacg ccatcgtaaa acgcgccttc accaacgttg ctgatgaccg tggcaaccgc
    18661 tttgcgatcg ataagctgct ctttatccat aactttggcg tagtcatggc tggtttcgat
    18721 cacttccggc agcgagccgc tacgtgaaag ggtgatgact ttggtgttat gagcgtcttc
    18781 ttccacgggc acttgtttaa cggtgatacc tgggttttct ttttcaaaac gggcaatcaa
    18841 tttgctgata actgcctgac gctcctgctc caccgaagaa tgcataaatt cgatggatac
    18901 atgggtttta ttttcttctt tacagcctga aatcagggcg caggaaacca gtgctgataa
    18961 cagcacgatt tttgatttaa tcatgagatg ttccttttta tttaatccac ataacctgcc
    19021 aggggcgcag cgttatttca ttacccaata tatcaacttc actaatcaga tcccgaccat
    19081 gcagatcagt aatatttaca tgctgaatat ttttactgac attaaacaat ccagtcagac
    19141 aattacca
//
LOCUS       DAJVPP010000083        18464 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVPP010000083 DAJVPP010000000
VERSION     DAJVPP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.083"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..1351
                     /locus_tag="OQM34_002877"
                     /pseudo
     CDS             1..1351
                     /locus_tag="OQM34_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-insensitive
                     transglycosylase/transpeptidase; frameshifted; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
     gene            1501..1932
                     /gene="ndk"
                     /locus_tag="OQM34_002878"
     CDS             1501..1932
                     /gene="ndk"
                     /locus_tag="OQM34_002878"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1453241.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2082..3236
                     /gene="trmG/rlmN"
                     /locus_tag="OQM34_002879"
     CDS             2082..3236
                     /gene="trmG/rlmN"
                     /locus_tag="OQM34_002879"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1453242.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3521..4534
                     /gene="rodZ"
                     /locus_tag="OQM34_002880"
     CDS             3521..4534
                     /gene="rodZ"
                     /locus_tag="OQM34_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1453243.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4561..5688
                     /gene="ispG"
                     /locus_tag="OQM34_002881"
                     /gene_synonym="gcpE"
     CDS             4561..5688
                     /gene="ispG"
                     /locus_tag="OQM34_002881"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1453244.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            5791..7065
                     /gene="hisS"
                     /locus_tag="OQM34_002882"
     CDS             5791..7065
                     /gene="hisS"
                     /locus_tag="OQM34_002882"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1453245.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7083..7703
                     /gene="yfgM"
                     /locus_tag="OQM34_002883"
     CDS             7083..7703
                     /gene="yfgM"
                     /locus_tag="OQM34_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1453246.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7714..8892
                     /gene="bamB"
                     /locus_tag="OQM34_002884"
     CDS             7714..8892
                     /gene="bamB"
                     /locus_tag="OQM34_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1453247.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9010..10482
                     /gene="der"
                     /locus_tag="OQM34_002885"
     CDS             9010..10482
                     /gene="der"
                     /locus_tag="OQM34_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1453248.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10552..10767
                     /locus_tag="OQM34_002886"
     CDS             10552..10767
                     /locus_tag="OQM34_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1453249.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10764..12134)
                     /gene="xseA"
                     /locus_tag="OQM34_002887"
     CDS             complement(10764..12134)
                     /gene="xseA"
                     /locus_tag="OQM34_002887"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1453250.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12296..13762
                     /gene="guaB"
                     /locus_tag="OQM34_002888"
     CDS             12296..13762
                     /gene="guaB"
                     /locus_tag="OQM34_002888"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1453251.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13831..15408
                     /gene="guaA"
                     /locus_tag="OQM34_002889"
     CDS             13831..15408
                     /gene="guaA"
                     /locus_tag="OQM34_002889"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1453252.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15503..16042)
                     /locus_tag="OQM34_002890"
     CDS             complement(15503..16042)
                     /locus_tag="OQM34_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS1453253.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16058..16576)
                     /locus_tag="OQM34_002891"
     CDS             complement(16058..16576)
                     /locus_tag="OQM34_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1453254.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(16887..17078)
                     /locus_tag="OQM34_002892"
     CDS             complement(16887..17078)
                     /locus_tag="OQM34_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCS1453255.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17096..17248
                     /locus_tag="OQM34_002893"
     CDS             17096..17248
                     /locus_tag="OQM34_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453256.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            17430..>18464
                     /gene="pdeF"
                     /locus_tag="OQM34_002894"
     CDS             17430..>18464
                     /gene="pdeF"
                     /locus_tag="OQM34_002894"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1453257.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALN"
BASE COUNT         4371 a         4483 c         5002 g         4608 t
ORIGIN      
        1 ttactacgct ggatgccggt cttcaacgac gtctggaaga actggcgcaa aactggaaag
       61 ggcggttgcc accgcgcagc tcactggcga tgatcgtggt taatcatacc gatatgcgtg
      121 ttcgcggctg ggtgggatcg gttgatctca acgatgattc acgctttggt catgttgata
      181 tggtcaatgc gatccgatcg ccaggatcgg tgctcaaacc gtttgtttat ggtctggcgc
      241 tggatgaagg cttgatccac ccggcatcac tgctgcaaga cgtcccccgg cgcaccggtg
      301 attatcgacc aggtaacttt gatagcggtt ttcatggccc gatcaacatg agcgaggcgc
      361 tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc acggcaaggc
      421 aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa tgtcgtcggg
      481 ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc cggatagtgc
      541 cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct atcgtgacgc
      601 ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca gacctgacgg
      661 cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga atcaggtcaa
      721 taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga actcagtcac
      781 tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg gtaactgtcg
      841 ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt tactgtcaga
      901 gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag ggcagcgcgt
      961 cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc taccgctgga
     1021 accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct cgacaatttg
     1081 cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc gcgatggcgc
     1141 gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat caagtggagg
     1201 ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg ggcgcaacgt
     1261 gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg atgtggggca
     1321 aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg ttgctaaaac
     1381 tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg ttgctcatta
     1441 taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac agaggtaaaa
     1501 atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa aaacgtcatt
     1561 ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac caaaatgctg
     1621 catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa accgttcttt
     1681 gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct ggaaggtgaa
     1741 aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa cgcactggct
     1801 ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca cggttctgat
     1861 tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga agtgtgcccg
     1921 cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg cgccagaatt
     1981 tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt ttttcaaccc
     2041 tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa caattagtca
     2101 cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc aaccgtcagc
     2161 agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat caggtgatga
     2221 agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc aacaaagtgt
     2281 tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt gaagaacagc
     2341 gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc gtcgaaacgg
     2401 tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg gggtgtgcgc
     2461 tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg cgggtatcgg
     2521 aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa gtcaccggtc
     2581 agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc aacctgaaca
     2641 acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg tctaaacgtc
     2701 gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc gatatgatcg
     2761 acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac gaaattgtgc
     2821 cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt tatctggaga
     2881 aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac cacgttaacg
     2941 acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg tgtaagatca
     3001 acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg aacagccgta
     3061 tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg cgtaaaacgc
     3121 gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc gaccgtacga
     3181 aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg gtctgatagc
     3241 cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga agtattaagt
     3301 tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca gtatgtaacg
     3361 atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac agacggtctt
     3421 atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag catgatggtt
     3481 caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg aagccacgca
     3541 cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg aacaactagg
     3601 acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg tacgcgacat
     3661 tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat atatccgctc
     3721 ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg aaaagcaggc
     3781 tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta aacgccgcaa
     3841 aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg ttatcggcct
     3901 gagcggtgcc tggtggtggc aagaccacaa agctcagcag gaagagatca ccactatggc
     3961 cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg ttccgttaaa
     4021 tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg tggatactac
     4081 cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg atccgcaaca
     4141 gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc cggtaccaac
     4201 ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg gcgtgaccac
     4261 gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct ggctggaggt
     4321 cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg gtaatttgaa
     4381 tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag tacagatcca
     4441 gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg ttgcgcgtct
     4501 gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg gagatttttc
     4561 atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt tgggaatgtg
     4621 ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg tacgacagat
     4681 gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga tatcgtccgt
     4741 gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca gcgggttaac
     4801 gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt agcggaatac
     4861 ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg tattcgcatg
     4921 gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa cgccggatcg
     4981 ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt gctggaatcc
     5041 gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa agtcagcgtg
     5101 aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa acagatcgat
     5161 cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc agtaaaatcc
     5221 gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg tgtatcgctg
     5281 gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc gctgcgtatc
     5341 cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga atttgatgtt
     5401 atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc gatggacgtt
     5461 tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac actcggcgtc
     5521 accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga ccgtctggac
     5581 aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag tcagctggac
     5641 gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg ggaagcgcct
     5701 cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa gcgttgaggg
     5761 ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc cattcgcggc
     5821 atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg cacactgaaa
     5881 aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga gcagaccccg
     5941 ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat gtacaccttt
     6001 gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg ctgtgtacgc
     6061 gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta tatcgggccg
     6121 atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca gttgggctgc
     6181 gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct cactgcccgc
     6241 tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc tatcggttcg
     6301 ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca gcataaagaa
     6361 aagctggacg aagactgcaa acgccgcatg tacactaacc cgctgcgcgt gctggattca
     6421 aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga ttatctggac
     6481 gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc ggggatcgct
     6541 tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac cgttttcgag
     6601 tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg ttatgacggt
     6661 cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat gggcctcgaa
     6721 cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc tgttgtcgat
     6781 atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt agctgagcgt
     6841 ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg caactttaag
     6901 aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct gggtgagtct
     6961 gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca aacggcagtt
     7021 gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag gagaaggaca
     7081 gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc ttttttgctg
     7141 aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg attggctggc
     7201 gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc tatcaaaatg
     7261 cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa aaatttgctg
     7321 ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag caatttgttg
     7381 acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca gacacgagcg
     7441 atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta cagctcaagc
     7501 aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg gctgccattg
     7561 ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt gcgcgtagtg
     7621 catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg atgcagatga
     7681 aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt actgctgcca
     7741 ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga agaagatgtg
     7801 gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac ggcgtggagc
     7861 acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc actggcggac
     7921 aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc ggatgatggc
     7981 aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa agagcctgca
     8041 ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag cgaaaaggcg
     8101 caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa agtcgcgggt
     8161 gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac cagtaacggt
     8221 cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa cctcgatatg
     8281 ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc ggccgtcgtg
     8341 ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat gatttggcag
     8401 cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga tgttgacacg
     8461 actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa cctgacggcg
     8521 cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt gaatgatttc
     8581 atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat ggcgttgacc
     8641 attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct gctgacttct
     8701 ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct gcactggatt
     8761 aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg tttccagact
     8821 gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac cgtgtactct
     8881 attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca ggggccgttt
     8941 tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta taaaaatgag
     9001 gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg aaaatccacg
     9061 ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc gggtctgact
     9121 cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg tattgatacc
     9181 ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca gtcgctgctg
     9241 gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg cctgatgccg
     9301 gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt cctggtggta
     9361 aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc gcttggttta
     9421 ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct gctggagcat
     9481 gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga agacgctgaa
     9541 tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga cgacttcgac
     9601 ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg taagtctaca
     9661 ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc tggcacgacg
     9721 cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct cattgacacc
     9781 gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc cgtaatcaaa
     9841 acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc gcgcgaaggt
     9901 atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg ctcacttgtc
     9961 attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt gaaagaaacg
    10021 ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc tgccttgcac
    10081 ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag ctccacccgt
    10141 cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga tcaccaaccg
    10201 ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg ttataacccg
    10261 ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta caagcgctac
    10321 ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg tattcagttc
    10381 aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac ccagatgcgt
    10441 aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt aagtatttta
    10501 tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca tatggaactg
    10561 cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg ttgcccatcc
    10621 tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc gttgcaggtg
    10681 ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt gatatcgaaa
    10741 aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt taccggctgg
    10801 atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag catttcaccc
    10861 gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt aacgctgtat
    10921 ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc attaccgaaa
    10981 cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata ttccagttgc
    11041 tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt cagccgctgt
    11101 gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc aaagctcatc
    11161 cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg cggatgctgc
    11221 tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag ataataatcc
    11281 atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc ttgctgatta
    11341 cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc aacaaaatcg
    11401 gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg gctggcaaaa
    11461 atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga accgccgccg
    11521 cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat ggcgcgaaca
    11581 atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac cggtagagaa
    11641 ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt ttttgaggtg
    11701 atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg atcgaacaaa
    11761 ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag cccttcaccg
    11821 gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta gagcgtaata
    11881 ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg gcggttgctg
    11941 ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa gtaccagtga
    12001 ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg tcccatctca
    12061 tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat tgcaggggat
    12121 tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc agtcgatagt
    12181 aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc aatcggttac
    12241 gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat tgcccatgct
    12301 acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg ctcattctac
    12361 cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc gtctgaatat
    12421 ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta ttgctctggc
    12481 tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc aggcagaaga
    12541 agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga ctgttctgcc
    12601 aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg cgggctatcc
    12661 ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg tgcgttttgt
    12721 taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc tggtcaccgt
    12781 gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac gcgttgaaaa
    12841 agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga aagacttcca
    12901 gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc gtgttggtgc
    12961 tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg ccgctggcgt
    13021 tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc agcgtatccg
    13081 tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg caacagctgc
    13141 aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta tcggccctgg
    13201 ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca ctgccgttgc
    13261 tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg gtggtattcg
    13321 cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga tggtaggttc
    13381 catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg gccgttctta
    13441 taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct ctgaccgtta
    13501 tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag gtcgcgtagc
    13561 ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc gctcctgtat
    13621 gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt ttgtacgtat
    13681 cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta aagagtcccc
    13741 gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg atttatatta
    13801 tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa gcatcgcatc
    13861 ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt gcgtgagctg
    13921 ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg tgacttcaat
    13981 ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa cagtccgcgt
    14041 gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta tggcatgcag
    14101 accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga atttggctac
    14161 gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga tgcgctgacc
    14221 gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt taccgctatc
    14281 ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat tatggctaac
    14341 gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac ccgccagggt
    14401 atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct gtggacgcca
    14461 gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga cgataaagtc
    14521 atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct gcaccgtgct
    14581 atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct caacgaagca
    14641 gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca cgtaccggca
    14701 gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa acgtaaaatc
    14761 atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga agacgtgaag
    14821 tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc tgcaaccggt
    14881 aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga gatgaagatg
    14941 ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat tggtctggag
    15001 ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg ccttggcgtt
    15061 cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc tgacgccatc
    15121 ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc gttcaccgtc
    15181 ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga ctgggttgtc
    15241 tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca tctgccgtac
    15301 gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat ttcccgcgtg
    15361 gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt ttgatccctg
    15421 cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc aagaatcata
    15481 ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc tcttctgctt
    15541 tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt gcgtcagctt
    15601 cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg ataaattcgt
    15661 tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc tgaatctcta
    15721 agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg gcttcggcgt
    15781 ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt ttgttcgcgg
    15841 cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg cgctccgcga
    15901 cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct tctgcgacag
    15961 cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag gctaagatgg
    16021 cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca ggacatttgg
    16081 tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct aaacctgtcg
    16141 taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct tccttatatg
    16201 ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct gccgcgccta
    16261 cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa ccgacgttgt
    16321 ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt gcgttccgtt
    16381 tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata ttaacggttt
    16441 tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca tgatcgtcag
    16501 catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc agaagacatt
    16561 ttttaaattt catcactatt ccttgttata cacattacga aaaataagac atttaagatc
    16621 atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt attaaaattc
    16681 acgcagggaa taatttataa aaatatatca ttacaacata tatattaatt ttatatttat
    16741 ttcgcagcca aacaggataa acatcagtca ggacaaagac gtaaaattta agatgaaaga
    16801 atatctgccg ctttgataat cccaaagagc aaaagcatcc gacagcgaag tgccgttatg
    16861 ttctgtgaaa gtggagaagg taaccgctaa cgttgtaccg gtgattcgac ggagagtgtg
    16921 gattgctgag cttctttttt gctctgatgg tgctgccagg caccgacgga cgagtagata
    16981 aaacggccaa agaagaagat aaagctgatg agcagtacga tacgggtcat gcgactgtta
    17041 aatcggtgtc gttttcgcat actagttgcc tgactcacaa aaggttcctt gaagtatgtc
    17101 ccacgctgtg gacggtactt acattaaggc acaacaggac aaaatggtca attcttcgtt
    17161 atgtaaaaaa gcgtcagtgg atacatattt taatgttatg gaagttaatt taattattta
    17221 caatgatgat gtaataatga tgaattaaca tgataaacgt aataattcat taatcagggt
    17281 tattttgttt gatatatatc aattggaact ttcatatgac acttagaatc aatgctctct
    17341 atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat acacctcctt
    17401 caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat ataaaaatac
    17461 gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc attttcgccc
    17521 acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat ctgcctttgg
    17581 cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg atcaccttag
    17641 ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca ttaacggcta
    17701 attttattat cattatcatt ctctgttggg gcggttacag ggtctttact ccccggcgta
    17761 acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg cagatagtgt
    17821 ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga ttactggcga
    17881 gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc aattatcagg
    17941 ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt cgggtagtgc
    18001 gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt gatgccaaag
    18061 tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg ttgctgttat
    18121 gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg tcattattgc
    18181 tgcccctgat gatgtgggga gcaatgcgct atggttataa gctgatttcg ctgctctggg
    18241 cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt tatcctggct
    18301 ataccacgca gctgaccata acctcctcca gttatctggt attctctttt attgtcaatt
    18361 atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag cggcttgcgt
    18421 atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaat
//
LOCUS       DAJVPP010000084        17082 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVPP010000084 DAJVPP010000000
VERSION     DAJVPP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.084"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..499
                     /locus_tag="OQM34_002895"
     CDS             <1..499
                     /locus_tag="OQM34_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCS1453258.1"
                     /translation="KPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQP
                     IVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNR
                     ARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSR
                     AWTVK"
     gene            complement(500..1660)
                     /gene="ybdL"
                     /locus_tag="OQM34_002896"
     CDS             complement(500..1660)
                     /gene="ybdL"
                     /locus_tag="OQM34_002896"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCS1453259.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAILLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1769..2857
                     /gene="hcxA"
                     /locus_tag="OQM34_002897"
     CDS             1769..2857
                     /gene="hcxA"
                     /locus_tag="OQM34_002897"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCS1453260.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2867..3064)
                     /locus_tag="OQM34_002898"
     CDS             complement(2867..3064)
                     /locus_tag="OQM34_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1453261.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3246..5351)
                     /gene="cstA"
                     /locus_tag="OQM34_002899"
     CDS             complement(3246..5351)
                     /gene="cstA"
                     /locus_tag="OQM34_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCS1453262.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5532..5945)
                     /gene="entH"
                     /locus_tag="OQM34_002900"
     CDS             complement(5532..5945)
                     /gene="entH"
                     /locus_tag="OQM34_002900"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCS1453263.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5948..6694)
                     /gene="entA"
                     /locus_tag="OQM34_002901"
     CDS             complement(5948..6694)
                     /gene="entA"
                     /locus_tag="OQM34_002901"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCS1453264.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSLGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6694..7551)
                     /gene="entB"
                     /locus_tag="OQM34_002902"
     CDS             complement(6694..7551)
                     /gene="entB"
                     /locus_tag="OQM34_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCS1453265.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7565..9175)
                     /gene="entE"
                     /locus_tag="OQM34_002903"
     CDS             complement(7565..9175)
                     /gene="entE"
                     /locus_tag="OQM34_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCS1453266.1"
                     /translation="MSILFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9185..10360)
                     /gene="entC"
                     /locus_tag="OQM34_002904"
                     /pseudo
     CDS             complement(9185..10360)
                     /gene="entC"
                     /locus_tag="OQM34_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10652..11608
                     /gene="fepB"
                     /locus_tag="OQM34_002905"
     CDS             10652..11608
                     /gene="fepB"
                     /locus_tag="OQM34_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1453267.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITEHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11612..12844)
                     /gene="entS"
                     /locus_tag="OQM34_002906"
     CDS             complement(11612..12844)
                     /gene="entS"
                     /locus_tag="OQM34_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCS1453268.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12955..13959
                     /gene="fepD"
                     /locus_tag="OQM34_002907"
     CDS             12955..13959
                     /gene="fepD"
                     /locus_tag="OQM34_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCS1453269.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13956..14948
                     /gene="fepG"
                     /locus_tag="OQM34_002908"
     CDS             13956..14948
                     /gene="fepG"
                     /locus_tag="OQM34_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCS1453270.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMCLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14945..15760
                     /gene="fepC"
                     /locus_tag="OQM34_002909"
     CDS             14945..15760
                     /gene="fepC"
                     /locus_tag="OQM34_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1453271.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15757..16890)
                     /gene="fepE"
                     /locus_tag="OQM34_002910"
                     /pseudo
     CDS             complement(15757..16890)
                     /gene="fepE"
                     /locus_tag="OQM34_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3991 a         4863 c         4442 g         3786 t
ORIGIN      
        1 aaagccagtg gattgcgttc tgtgggttaa aaacagccaa cttatgccta atgattacaa
       61 cccaaacaac gtggcaccac ccgaaaaaaa actgctgcaa aaatccattg aaatagatgg
      121 ttttacccaa cccattgtgg tcacacatac ggataaaaat gccatggaaa tcgtggatgg
      181 ttttcaccgc catgaaattg gtaaaggctc atcatcatta aaattacgct taaaaggtta
      241 tttgccggtg acctgtctgg aaggtacgcg taatcaacgc atcgccgcga ctattcgtca
      301 taatcgcgct cgtggacgcc atcaaatcac cgccatgtcg gaaatcgtcc gtgaacttag
      361 ccagttggga tgggatgata ataaaattgg caaagagctg ggaatggaca gcgacgaagt
      421 gctgcgactg aagcaaatta acggcctgca agagttgttt gccgaccgtc aatattcccg
      481 cgcctggacg gttaaatagc taaagctggc gcaggcgttc agctgctgcc agcaacgtcg
      541 attccttctt ggcaaaacag agacgaatca gtttatgtgg gaagggatcg gcgcaaaaca
      601 ccgacagcag aatcgccgct acgccgtgct cctgcgtcag ccactggcaa aactcaacat
      661 catccagggt agaaaccgcg ctgtaatcca ccagcaaaaa gtatgtacct tcacacggta
      721 aaatctccag ccggctttca tttaaagcat tcaccagaat atcgcgcttc tggcgataaa
      781 agtccggtaa cgcaagataa tgctcaggtt ctgcacgtag catatcagca agcgccagct
      841 gtgccggggt attcaccgaa aaggtcagat actgatgtac cttgcgaatt tcggcgctga
      901 tgggcgctgg cgcaacacaa taacccactt tccagccggt catatgatag gtcttgccaa
      961 atgaagaaac cgccactgcc cgctcacgca gctgcggatg cgccagcaca ctggcatggc
     1021 cctgttgtga aaagttgatg tgctcgtaga cttcatcgct aatgacaaaa atctcgtgcc
     1081 cggcgatcgc ctgccacaaa gcggcgaaat cagcctgctg ccagacagtt gcactggggt
     1141 tatgcggagt gttgaggatc accagtctgg tgcgctcgct taataatgcg gcaaattcct
     1201 gccagtcaac gcgaaaatgc ggtggttgca gtgccatacg cttcactatt cccccagaaa
     1261 gcgcgatggc gggggcgtaa ctgtcatagc tgggatcaaa acaaatcact tcatcgccat
     1321 tgcgcaccag tgcggtaatc gccgcgtata acgcttccgt cgcccctgcc gttacggtga
     1381 tatcgctatc ggcatctggt tgatagccat acaaacgttc cgttttctga gcaatcgcct
     1441 cgcgcaaggc ctgcacgccg gtcatgggcg cgtattggtt tgccccctgg gcaacgtggt
     1501 acgccagccg ctcctgtaaa tagcgcggac catcaaaatc aggaaagcct tgcgacaggt
     1561 taatcgcctg gtgttgctgc gccagcgcgc tcatctgggt gaaaatagtg gtgccaagtt
     1621 gtggaagttt gctttgtgga atcagagggt tatttgtcat ttttattgta cctgacgaag
     1681 ttgcgtgttg aaggcactat aacacgatgc cagtatttgg caatcaagac gtttagatgt
     1741 ctaaataaaa caataaggac aacacaacat gcctcacaat cctatccgcg tggtcgtcgg
     1801 cccggctaac tacttttcac atccaggaag tttcaatcac ctgcacaatt ttttcactga
     1861 tgaacaactt tctcgcgcgg tgtggatcta cggcaaacgc gccattgctg cggcgcaaac
     1921 caaacttccg ccagcgtttg gacttccagg ggcaaagcat attttgtttc gtggtcattg
     1981 cagcgaaagc gatgtacagc aactggcggc tgagtccggt gacgatcgca gcgtggtgat
     2041 tggcgtcggc ggcggcgcac tgctcgacac cgcgaaagcc ctcgcccgcc gtctcggtct
     2101 gccatttgtt gccgctccga cgatcgccgc cacctgcgca gcctggacac cgctctccgt
     2161 ctggtataac gatgccggac aggcgctgca ttatgagatt ttcgacgacg ccaattttat
     2221 ggtgctggtg gaaccggaga ttatcctcaa tgcaccgcaa caatatctgc tggcggggat
     2281 cggtgacacg ctggcgaaat ggtatgaagc ggtggtgctg gctccgcaac cagaaacgtt
     2341 gccgctaacc gtgcggctgg ggatcaataa tgcgcaggcc attcgcgacg tcttgttaaa
     2401 cagtagcgaa caggcacttg ccgatcagca aaatcagcag ttaacgcaat cattttgcga
     2461 tgtggtggat gctattattg ctggtggtgg gatggtcggt ggtctgggcg atcgttttac
     2521 gcgtgtggcg gcggctcatg ccgtgcataa cggtctgact gtcctgccgc aaaccgagaa
     2581 gtttcttcac ggcaccaaag tcgcttacgg aattctggtg caaagcgcct tgctgggtca
     2641 ggatgatgtg ctggcgcaat taaccggggc gtatcagcgt tttcatctgc cgactacgct
     2701 ggcggagctg gaggtggata tcaataatca ggcggagatc gacaaagtga ttgcccacac
     2761 cttgcgcccg gtggagtcca ttcattacct gccagtcacg ctgacaccag atacgttgcg
     2821 tgcagcgttc gaaaaagtgg aatcgtttaa agcctgacgt gcaaatttag cagcagcgcg
     2881 cgccgccttt tccaccgtag cgcgcgtcct gccgctcacg gaaaaactcc tcgtaggtca
     2941 tcggcgtttg atcgggatga ttaacccgca tatgttcgac atagttgtcg taatcaggca
     3001 taccaatcat cagcttcgcc gcctgaccta aatattttcc ggcttttgcc agtgaatcaa
     3061 acatcttggc ctccgctaac catgttgctg aaagagtgcc ggatgcggcg tgaacgcttt
     3121 atccggccta caaatgctat caacgcactc ggtcagtccc ctcgccccat cggggagagg
     3181 gttagggtga gggggaaccc tcggcactat gccaacattc gccaacatcc cccctcactc
     3241 tgactttagt gcgcgccttt cgcctgcgct acgatctcct cgacattttc cggcatcggc
     3301 tcatacggcg tttctttcgc cgttggcttc ggatctttca atgccgcaag tgccgtctta
     3361 atcgagaaca gtgccaggac caccacgacc accataaaga agatggttaa cccggcatcc
     3421 agacggttgt tgaacaccag ttgcgccagc tgtgactcag tatactgcga cggaatattg
     3481 ccgctgtcga tcattgcctg gaacttgtta gcaatggcca ggaagccgac tttcgcatcc
     3541 gggctaaacg ctttctgcca gtcagcggtc agggtacaaa tcagcagcca ggccgttggt
     3601 accagcgcca cccaggcgta acgttgacgt ttcatcttga acaacaccac ggcacagagc
     3661 atcagcgcca tccctgccag catctggttg gcaataccaa acagcggcca cagagtgtta
     3721 ataccgccca gcggatcgac cacgctctga tggaggaagt agccccacgc cagtacgcac
     3781 agcgctgttg ccagcaggtt agcaggcagt gaatcggtcc gcttcaggcc aggagacacc
     3841 acgcccagca gatcctgcaa cataaagcgc gcagcacgcg tacccgcatc taccgccgtc
     3901 agaataaaca gagcttcaaa cagaatggcg aagtgatacc agaacgccac atccatcata
     3961 ccacccagcg caccgtgcag gatgtaagcc atccccaccg ccaacgtcgg cgcaccgccc
     4021 gcacgggaaa tgatcgactg ttcacccact tcgctggcaa tctggttgag cgtatctggt
     4081 gtaatgctaa agccccagct actcaccacc tgcgcggcag aagcgactac atccgccgtc
     4141 cctgccggag ccagcaccgc cattgggctg ttcatggcaa aatacacgcc cggatcgatg
     4201 atacaggcag aaactagcgc cataatcgcc acgaaggatt ccatcaacat cccaccgtag
     4261 ccgataaagc acgcctgccc ttcgttcgcc agcatcttcg gcgtggtgcc ggaagagatc
     4321 agcgcatgga agccagacac cgcaccacag gcgatggtga taaacaggaa cgggaacagg
     4381 ttaccggtcc ataccgggcc agtgccatcg acaaatttgg tcagcgcagg catggtcagc
     4441 gtcgggcgca taatcaaaat gcctaccgcc agaccgacga tagtcccgat tttcaggaag
     4501 gtcgagagat agtcacgcgg ggccagcagc aaccacaccg gcagcaccgc cgccacaaaa
     4561 ccgtagccca ccagcatcca ggtcagctgc acgccggtaa agtcaaagta cggtgcccag
     4621 gtcggacttt ctgccaccca gccgccagag ataatggcga aaatcaggaa caccaggcca
     4681 atcactgaca cttcgccaat gcggcccgga cgcagatagc gcaggtagat tcccataaaa
     4741 atcgccagcg gaatggtgaa cgcaacggta tacgttcccc acgggctatg agtcagggct
     4801 ttcaccacga tcatcgccag tactgcaagg ataatgacca tgatcataaa gcaggctacc
     4861 agcgcaatca ccccggcagt tggccccatc tcttctttga ccagctcacc cagcgagcga
     4921 ccgtcacggc gcgtagaaac aaacagcacc atgaaatcct gcaccgcacc ggcgagaacc
     4981 accccggcga gcagccagat catccccggc aggtagccca tttgcgctgc cagcaccggc
     5041 cccaccagcg gccctgctcc ggcaatggcc gcaaaatggt gaccgaacag cactttcttg
     5101 tccgtcggca catagtccag cccgtcgtta tggcgcaccg ctggcgtcat acgcgtcggg
     5161 tcaaccgcca gcacattttt ggcgatatac agcccataaa aacggtaagc gatcagatag
     5221 atacagaccg acgccaccac aatccacagc gcgttgatct gttccccacg atttaaagca
     5281 atgtatccca gagcaaatgc tcccattaca gagagcactg tccagacgag gtatttccct
     5341 gatttgttca tagttgttat ccgtgtgcgt gtccatagag atgttacatt ttgtttctat
     5401 aacatctttg tacgatttaa caacacggta accatgtaaa tcggagttga aaccaatatt
     5461 taacttaaca atgttaactc gatcactccg ttaaccgagt tttcctgcaa tctcatttaa
     5521 ttctgtcggg ctcatcccaa aactgccgta cccagccgac aagtgcagca acgccgcccc
     5581 tgttcatcga aaacgacgat ttcccagctc tgattttgcc gcccaagatg cagcggctgg
     5641 cagacgccgc gtaccttgcc ttcagacacc gggcgatgat gggtggcgtt aagttccgtg
     5701 cccaccacac actgcccgtc acgggtcatc ataaatccgg ccatcgatcc cagcgtttca
     5761 gccagcgccg ccgacgcgcc gccatgcagc aggccaaacg gctgatgagt acgggtatca
     5821 accggcattt cggcttccag cacatcatct cccagacggg tatacacaat tcccagatgc
     5881 gccaccattg tgttatcgct ggtggcgttc agttcgtcga gcgttaaatg gcgtttccag
     5941 atcatgctta tgcccccagc gttgagccgc catcgaccac aatatcctgt agggtgatat
     6001 ggctggcgag gtcagaggcg aggaacaaaa tcgtgttggc gatctcttgt ggacgggcga
     6061 ttttccccag cggaatgccg agtttaaact gctcgccaaa gccgcgaata cgctgttctt
     6121 cggcgtcatc gctcacccac agcgtgcgtt gcatatcggt gtcggtggag ccaagcgaaa
     6181 ccacattaca gcgcacgccg ctacccgcca gttccagccc gacgctcaac gccaggcttt
     6241 tcagcgccgc tttcgatgcg ccataagcac tcataccaat acgcgccgtg tgtgcggcgt
     6301 cggacgccac agtgacaatc gccccgcccc gctgacggcg aaactggttc atggtttgct
     6361 ggaacaggtt aaacgcaccg ccgacgttaa ccgcaaaagt ctgctgccag tcctctttac
     6421 tgagctgatc ggtcgcgccc atgcgcaaaa ttcccgccgc attgatcagc acgtccagtc
     6481 gctccgtttc agctaacagt cgctgacata cctgcgccac ctgcccggcg tcggcaacat
     6541 ccattacttc ggtcgcaaag ggatattgct cctgagtgaa cgcttgatca aaacctgtaa
     6601 ctttcgctcc cgcctcaaca aacgccagcg ccgtggcgta gccgatacct ttacctgcgc
     6661 cggttaccca gacattttta ccgctgaaat ccattatttc acctcgcggg agagtagctt
     6721 ccaccaggcg tcgatggtcg ggtttttcgc cagcatgaca aagtcgatat caccatgcac
     6781 tttgcgccag cgcgccgcca gcgccatcat gcgcaccgaa tccagaccgt agtcgatcag
     6841 gttgtcgtca tcgaacggtt catcggactc gtccagcaac ggcaggatca cctcacgcag
     6901 cgccgctttg ctggcgggga taggtgctgg cagtaattct tcggtcatca ccacccggcc
     6961 agaacgtccg gccacatatt tcagcgacat caaatgctcg tcacggctga aatcagccag
     7021 cgcatccgcc accataaacg gtttaatatc gcgcataaat gcgtcggttg cggtggtcat
     7081 acagccaatg tgggcatata ctcctgtaat aatcagctgg ttacgtccac tctctttgag
     7141 catttgctcc agcggagaac gatgaaacgc gctgtagcgc cacttcacca gcaccgtgtc
     7201 gtcggcatct ggcgtcaggc gatccaccac cttttgctgt tccggcgagc gggtcaggcc
     7261 cggcccccac atatcattca acagcgcccg atcttcatcg ctctgctctt tcggctgggc
     7321 ggtgtaataa accgggatat tgtgctgttt gcagtagtcg cgcagcgcag caatattcgc
     7381 gatcacctgc tccatcatcg ggcagttctc gccccagaag ctgacaaaat agtcctgcat
     7441 atcatggatt aacaacgcgg cacgttgcgg ttcaaaggcc cagtcaactt tattctgcgg
     7501 aatatcgtga gactccggca gtgcgtaagc ctgtaatttt ggaatagcca tcgtgttctc
     7561 tccttcaggc tgatgcgcgt gacgccagcc actgacgtaa ttgtttttta tcgactttcc
     7621 cgaccgccgt cagcggaagt gaatccacac actccacgcg atccggtaat ttaaattcgg
     7681 caataccctg ttcacgcagg aaacgacgca cctgcaccgc gcgcagcggc tcttttacca
     7741 ccagataagc acagcttttt tcgcccatca gctcattttc catgctcacc agtgcggcat
     7801 agatcactgc cggatggctc agcagcaggt tttcgatatc ttcggcagcg atcttctcgc
     7861 cgccacggtt gatctgatct ttctcgcgcc cctgcacggt gatgtacccc tctggatcaa
     7921 tagagatcag atcgccggaa cagtaaaaac cgttggcatc aaaggcactg gcattgtgct
     7981 gcggactttt gtaatagcca cggaaggtgt acggcccgcg cgtcatcagg cgtccaactt
     8041 ccccttgcgg cagtggattt ccttcggcat cggcaaccca cacttcatcg tccggacaca
     8101 ttgggtaacc ctgggtatgg ataattttct ccgcgctatc atcaagacgg gtgtagttca
     8161 ccagcccttc cgccatgcca aacacctgct gcaactggca gccaatctca gcgggaatac
     8221 gcgctgcaag cgtggcagaa agacgtgcgc cgccgacctg taacagtttc agcgaggcaa
     8281 gctgcgcccg gctttcgcct tcagccagcg cctgcaacca caggctgact gccggcggca
     8341 ccagcgcggt aacgttaacc tgatgttttg caatcaacgg gaagcaaagc gtggcgctgg
     8401 gatcggccgc cagaacaacc gttcctccgg caagaaagac gcccagcgat cctggcgaac
     8461 tcatagcgta gttatgagcc gccgggatcg cgcacaggta gcgcgtctgt tgtgtgaact
     8521 gacaaatctc ggcgctacga cgcacgctgt agtaatagtc gttatgagtg cgcgggatca
     8581 gtttcggtgt gccggtggtg ccgccggaaa gctggaaata ggccacttca tcagcaggtg
     8641 atggcgtggc agtaaaaccc tctgccggat ggttaatcgc atcctgcaag ttatgctcac
     8701 cgctgtcgtt gtgcagttgc accacgcgaa tggaagaatg ctctgcaaca aatgtgttga
     8761 ggaaatcatc cccgctaaat agcgcatgct ggcgatcggc aatcagtaac gccggttcaa
     8821 tctggctggc gtaggcgttc agttcactac gctgatggct gaacagcgcc agcaccggcg
     8881 cgacgcccag tttcagcagc gcgaaaaagg taatgtaaag ttcagcgacg ttacccagtt
     8941 gtaccagcgc ggtttcacca ggtttaatgc cctgacggcg caaactacag gcgaggttat
     9001 ccgccgcctg attcagctcc cgataactca actgccgctc gccgtcgata accgcgatgc
     9061 tgtcactcgc agcgtggcga gtcagaatgt cggtcagtgg caaatcctgc cagtagcctt
     9121 tttcccgata gcgacgggca aactcttccg gccagcgggt gaatagaatg ctcatcctcg
     9181 ctccttaatg taatccaaaa acgttcaaca tggtagaaag tttgacgcct gtttcgcgcc
     9241 actcacccaa cggtgacgac gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat
     9301 tttcccgcag cttcgcgcag cggatggtca ccacccattc gccgttacct tcgctgtcac
     9361 accaacccac aatgccgcca aacagttcgc ggtcgaacgg ctccagttca gcaataacct
     9421 gggtcgcggc ctgatgcggg aaaccgctca gcgcgggggt cggatgcagc agacaggcca
     9481 gagtcagtgc gttttcttgc gaattcgctt taccttcaaa gggagttgcg agatgccaca
     9541 gcgtcggcgt ggtaatcaat tgtggagagg agggaacgtg taactcacta ctgcgttcgc
     9601 gcagtacctc tttcatcgcc tgagtcacca gttcatgttc atggcgatct ttttctgacg
     9661 ccagcagacg attacccgct tcgcgatcga gcacttcatc cggctgacga cgcgcggaac
     9721 cggctaacgg aatggagcta aaacgctcgc cgtctttacg tagcagcagt tccgggctgg
     9781 cccccagcag gacgccacca tcagccagcg ggacatggaa gttgtaacta accgggtttt
     9841 gcgcaatcaa ccgttccagc aatacgccac tatcaatggc ggcgtcagtg gtgatatcaa
     9901 tcaatcgtga caacaccact ttgtcgacct gcggcgtggc ggtaagtgcg gcggcgcggg
     9961 cacccatctg ttcaaacgtg gtttgttcag gaattgcctg gcgttccacc acgttcagcg
    10021 actggctgcg ggtaaaacag cgggctgagg tctgtttttc ctgacgggag aacgactgct
    10081 aggattcggg aatatacagc gacgaaggct gacgtggatc gaagggaatc gccccgacca
    10141 tcaccggatt tttgatgccc tgcgctttgg catcggcaaa cagcgcggcg agtttttgct
    10201 ggaagggact gtcgggcgaa tccccgttca cagccggttc atcgaaacgg gcgaaacatc
    10261 ctgacgtcgt aaaactgcgg tacggcgaca taaagaaaaa gcgattgggc gcaagtgttg
    10321 ccatggtctg ctgtacttcc tcagccagtg acgtatccat atcatcctcc acaaaatgat
    10381 aaaggcttta ataatgatta tcacttatat tttcggccgc taacctaaaa gcaaacgcgc
    10441 actatgtcaa ctcttgaggt aacgcaatct gcaactcggg gttgcatctg gttgtggtta
    10501 caatgaaaat gagaagcatt attgatgaat tcgcataagc gcaatgtgat gtcctgcgcc
    10561 gttttgcccc ctctccccaa agggacggat tgtgcgcttt gtcgaatttg tcattacgcc
    10621 cttaacctta ttaataacag gaagctgact tgtgagactc gccccgctct accgcaacgc
    10681 ccttctatta acaggacttt tgctttcagg aatagccgta gttcaggccg ccgactggcc
    10741 gcgtcagatt actgacagcc ggggcactca taccctggaa agccagccgc agcgtattgt
    10801 ttccaccagc gtcaccctga ccggatcact gctggcgatt gatgctccgg tgatcgccag
    10861 cggcgcgacc acgccgaata accgcgtcgc agatgaccag ggctttttac gccagtggag
    10921 caaggtggcg aaagaacgca aactgcaacg gctctatatc ggcgaaccga gcgccgaagc
    10981 cgttgccgcg caaatgccgg atctgatttt aattagcgca accggcgggg attcggcgct
    11041 ggcgctgtat gatcagcttt ccaccatcgc cccgacatta atcatcaatt acgacgacaa
    11101 aagctggcag tcgctgttaa cgcagcttgg cgaaattacc gagcatgaga aacaagcggc
    11161 agagcggatt gcacagtttg ataagcaact ggcagcggcg aaagagcaaa tcaaattacc
    11221 gccgcagccg gtcactgcca ttgtctatac cgccgctgca cacagtgcca atctctggac
    11281 gccggaatca gcacaagggc agatgctgga acaactcggc tttacgctgg cgaaattgcc
    11341 cgcagggtta aacgccagcc aaagccaggg caaacgccat gacatcattc agcttggtgg
    11401 ggaaaatctg gctgcggggt taaacggtga atcgctattc ctgttcgctg gtgatcagaa
    11461 agatgccgat gcgatttatg ccaatccact gctcgcgcac ctgcctgcgg tacaaaacaa
    11521 gcaggtttat gcgctgggaa ccgagacgtt ccgcctggat tactacagcg ccatgcaagt
    11581 gctggaacgg cttaaggcgc tgttttaagc attaactgtc ggacgctgtc acctgcggcg
    11641 gcgtctggcg aaaacgtcgc aactccacca gcaccagcaa taacaacacg ccgataatca
    11701 acaaaccaaa accgctcgcg cttgcggaag caaccggtgt catcatcgcg cccaaaccac
    11761 ccagcagcgc cgcgcctatg gcatcgcccg tcacgttctg cgccgtccac aaaccgttaa
    11821 tccgccctaa catcgcttcc ggggtttgcg tttgcagcat tgtatattgc agcaacgaac
    11881 taaccgcgct cagccagccg aacagcgcca gacaaaccac gcctaaaatc cacatcggca
    11941 tcaggccaaa cagaccaatg gcgaggaacg atcccagcgt ggagagcagc atcaataacc
    12001 ctggtcgcgc actatgtgcc agcttcccgc tggttaacgc accaatagcc gcgccgagcg
    12061 ggatcgccgc gtagagaaaa ccaatctgtg ccgctgacat ctgccagttg tcagccagcg
    12121 ccggatacag taccagcacc gcgctcgcca tcgtcaataa accacccagc agcgggctgg
    12181 caagcagaaa acgaaatcct gccagtaatg atttcaacgg atgctcaagc ggctgcggtg
    12241 gcggtggcaa cgccggaagg cttaataacg gtaacaaggt aataaacgtg cccgccgccg
    12301 ccagcccgta gttccaggct acgccgccgg tcgccagcaa taaaccgcca atcatgggcg
    12361 aaatcaccga ccccaggcgc acggtcaaca tggtgatcgc cccggcctgc attaagtttt
    12421 cacgccctac cagtgcagat gttgccgcca gtagcgccgt aacaccaagt gatgcgaaaa
    12481 aaccatccca taaaccaagt aaatagattg ccagcaatga cggctccggc agcagtgcat
    12541 taaggcacag tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa atcacttttt
    12601 tgcgttcata gcgatctgcc agcacaccgc caaccatcag gccaacaaac atcgcgccgc
    12661 cggtcagcgt caccgaaagc cccacctgcc aggtagaatg ggtcatcatc tggatctgca
    12721 ccggcaccgc gacgccgagc aaacccagag acacaattga gataaaacga gcgaggaata
    12781 ctgcgcgaaa cgccgggtgc gttttcaaca ggctgaggtt aagcagccag gattgtttat
    12841 tcattacaat gccttgccat caaatatttt aaatacctat tcttaggctg cacatgttaa
    12901 catatccaaa taagatcgat aacgataatt aatttcatta tcatggaagt tcgtatgtct
    12961 ggttctgttg ccgtgacacg cgccattgcc gtgcccggat tgctgttatt actgattatc
    13021 gcgacggcat taagcctgct cattggggca aaatcactcc ccgcttccgt agtgctggag
    13081 gccttctccg gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc gcggctgccg
    13141 cgtacccttg ccggtttact ggcaggcggc gcgcttggcc ttgccggggc gttaatgcaa
    13201 accctcaccc gaaacccact tgccgacccc ggcttgcttg gcgtgaacgc cggagccagc
    13261 tttgccattg tgctgggtgc ggcgctgttt ggttactcat ccgcacagga acaactggcg
    13321 atggccttcg ccggggcgct ggtggcctca ttgattgttg cctttaccgg cagtcagggt
    13381 ggagggcagt taagtccggt gcgtttaacc ctggcgggcg tggcgctggc ggcggtgctg
    13441 gaaggactga ccagcggcat cgccctgctt aatcctgacg tctacgatca actgcgtttc
    13501 tggcaagccg gttcgctgga tattcgcaat ctacatacct taaaagtggt gctgatcccg
    13561 gttctgatcg ccggagcaac tgcgctatta ctgagtcgcg cgctgaacag tttgagcctc
    13621 ggcagtgaca ccgcgacggc gctgggcagt cgcgtggcgc gcacacagtt gattggtctg
    13681 ctggcgatta ccgtgctttg tggtagtgcg acggcggtag ttggcccgat tgcctttatc
    13741 ggcctgatga tgccgcacat ggcgcgctgg ctggtaggtg ccgatcatcg ctggtcgctg
    13801 cccgtcacgc tacttgctac ccctgccctg ctgctgtttg ccgatatcat cgggcgggtg
    13861 attgtgcccg gcgaactgcg cgtttctgtg gtcagtgcgt ttattggtgc accggtgctg
    13921 atcttcctcg tgcgacgtaa aacgcgaggt ggtgcatgat ttacgtctct cgccgattac
    13981 tcatcacctg tttgctgctg atttccgcct gtgtggttgc aggtatctgg ggattacgca
    14041 gcggtgccgt cacgctggaa atctcgcagg tattcgccgc gttgatgggc gatgcgccgc
    14101 gcagtatgac gatggtggtc accgaatggc gtttaccacg cgtgctgatg gcgctgttga
    14161 ttggcgcggc tctgggcgtc agcggcgcga tttttcagtc gctgatgcgt aacccgctcg
    14221 gcagccctga cgtaatgggc tttaacaccg gggcgtggag cggcgtgctg gtggcgatgg
    14281 tgctgtttgg tcaggatctg acggctatcg cgctggcagc aatggtgggc ggcattatca
    14341 cttcgctgtt ggtctggctg ctcgcctggc gtaacggtat cgacaccttt cggttgatta
    14401 ttatcggtat cggcgttcgc gccatgctgg tggcctttaa tacctggctg ttgctgaaag
    14461 cgtctttaga aacggcgcta acagcaggtt tgtggaatgc cggatcgctc aacggcctga
    14521 cgtgggcaaa aacctcgcct tccgcaccca tcattatatt gatgctcatt gccgccacct
    14581 tgctggtgcg gcgaatgtgc ttgctggaga tgggcgatga taccgcctgc gcgctgggcg
    14641 tcagcgtcga gcgttcgcgt ctgttaatga tgctggttgc agtggtgctt accgctgcgg
    14701 caacggcgct tgccgggccg atttccttta ttgctttagt cgcgccgcac attgcccgac
    14761 gcattagcgg caccgctcgc tgggggctga cccaggcggc gctgtgcgga gcgctgttac
    14821 tgctggcggc cgatctctgc gcccaacaac tgtttatgcc gtatcaactt ccggttggcg
    14881 tcgttaccgt cagcctcggc ggtatttacc ttatcgtctt gttaattcag gagtctcgca
    14941 aaaaatgacc gaatcagtag cccgtttgcg cggcgaacag ttaaccctgg gatatggcaa
    15001 atataccgtt gcggaaaatc tgactgtaga aattcctgat ggtcacttca cggcaattat
    15061 cggaccaaat ggctgtggta aatccacgtt actgcgtacc ttaagccgcc tgatgacgcc
    15121 tgctcacggg catgtctggc tggatggcga gcacattcaa cattacgcca gtaaagaagt
    15181 tgcacgccgg attggtctgt tggcgcaaaa tgctaccacg ccgggcgata tcaccgtgca
    15241 ggagctggtg gcgcgtggac gttatccgca tcaaccgctg tttacccgct ggcgcaaaga
    15301 ggatgaagaa gcggtaacga aagcgatgca ggccacggga ataactcatc tggcagatca
    15361 aagcgtggat accctttctg gcggacaacg ccagcgagcg tggatcgcga tggtgctggc
    15421 ccaggaaacg gcaattatgc tgctcgacga acccacgacc tggctggata tcagtcatca
    15481 gattgatcta ctggaactgt taagcgaact gaaccgcgag aaaggctata ccctggcggc
    15541 ggtgctgcac gatcttaatc aggcctgtcg ttacgccagc catttgattg cattgcggga
    15601 agggaaaatt gttgctcagg gagcgccgaa ggagattgtc actgctgaac tgattgagcg
    15661 catttatggt ctgcgatgca tgatcattga cgatccggta gccggaatgc cgcttgtggt
    15721 gccgctcgga cgaacggcac cttcaaccgc aaatagttaa actaagtggt ctgccatcat
    15781 ggcatcctgt tttctggatg ccatcgcata gcgcaataac acgctaccac aagccaccat
    15841 cccgccgatc agcgcggaaa ggatcacaat aatcgcctta cccggaccgt cttttttcac
    15901 tggcaatgac gggcttaact gatatttaaa cggcgtaaaa ttcacatcgt tgacatttgc
    15961 ttttgttaat tgctcgacaa gatactgccg attacgtaat tcaccgttca gttccgcaac
    16021 gtcagtaacc gctttttcaa tttccagttt gcgttcaata ccgtctgcgc cgagagaaat
    16081 agagaaatcg gggtcatctt taacggcctg accattactg tatacgggct ttttaattcc
    16141 tgccgcgttg gcaatgtcga gtgaataatt gaggcgctga atgtttgcat caagttgatt
    16201 tttcgtttta atgcgatcct gagccagttt ttctttttca aactgggttt tgatctccag
    16261 tttattacgg acgttttcta tcgactcttt caccaccaac gcagagatat aatcgatata
    16321 cccgctcaaa acggtctgcg cctctttact ggttggcgcg gtaaaactta gcgtccagga
    16381 ggtatacagt gacggttcat cttttttctt actagcattg tcatcaaccg ctttcatttt
    16441 ttcgctcaat gcaacaatag cgcgatgcaa atccagttca tcgattttcg cctcttttaa
    16501 ttagtccatc acataaggtg atgaacgcag gtactcttcc agcaagctaa cagactgaaa
    16561 cttcttgata aacaggttaa atgcaccgcc acggtcgata ttaacatcga gatcaagaac
    16621 gcggagtttg gtaaaggttt tctccaggtc ctgccactga atggcttcgg cgggagtaat
    16681 aacggctgag ctggtccatt tttgcggcag gatgaaagag atcagtaaac ctgcgcaggc
    16741 aaatgcaaaa acgaccgcca tgaccgtttt tttggcccgc cataaaactt cgattagatt
    16801 aagtaaatca atttcattat tactgggcga cgccagagga tactcagaaa aatgagcgtc
    16861 acttccctgt ttaatattca atgatgacat ggggtataac ctgaaaatga gtccaatagg
    16921 ggaaaatctc cacggcgctc actttaccag ttggtgagaa ttatccgaag ctgaagtttg
    16981 taaattcctt ccactgaatg caacattcac tctatttaag agaaatattc atcaaaccta
    17041 taaatcaaac aaataaaaaa cccataatta ttttctgtaa tt
//
LOCUS       DAJVPP010000085        16753 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVPP010000085 DAJVPP010000000
VERSION     DAJVPP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.085"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            186..1004
                     /gene="ybhA"
                     /locus_tag="OQM34_002911"
     CDS             186..1004
                     /gene="ybhA"
                     /locus_tag="OQM34_002911"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCS1453272.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1005..2063)
                     /gene="modC"
                     /locus_tag="OQM34_002912"
     CDS             complement(1005..2063)
                     /gene="modC"
                     /locus_tag="OQM34_002912"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1453273.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2066..2755)
                     /gene="modB"
                     /locus_tag="OQM34_002913"
     CDS             complement(2066..2755)
                     /gene="modB"
                     /locus_tag="OQM34_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1453274.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2755..3528)
                     /gene="modA"
                     /locus_tag="OQM34_002914"
     CDS             complement(2755..3528)
                     /gene="modA"
                     /locus_tag="OQM34_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1453275.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3695..3844)
                     /gene="acrZ"
                     /locus_tag="OQM34_002915"
     CDS             complement(3695..3844)
                     /gene="acrZ"
                     /locus_tag="OQM34_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1453276.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3973..4761
                     /gene="modE"
                     /locus_tag="OQM34_002916"
     CDS             3973..4761
                     /gene="modE"
                     /locus_tag="OQM34_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1453277.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4829..6301
                     /gene="modF"
                     /locus_tag="OQM34_002917"
     CDS             4829..6301
                     /gene="modF"
                     /locus_tag="OQM34_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1453278.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTM
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6562..7578
                     /gene="galE"
                     /locus_tag="OQM34_002918"
     CDS             6562..7578
                     /gene="galE"
                     /locus_tag="OQM34_002918"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1453279.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7588..8634
                     /gene="galT"
                     /locus_tag="OQM34_002919"
     CDS             7588..8634
                     /gene="galT"
                     /locus_tag="OQM34_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1453280.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8638..9786
                     /gene="galK"
                     /locus_tag="OQM34_002920"
     CDS             8638..9786
                     /gene="galK"
                     /locus_tag="OQM34_002920"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1453281.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9780..10820
                     /gene="galM"
                     /locus_tag="OQM34_002921"
     CDS             9780..10820
                     /gene="galM"
                     /locus_tag="OQM34_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1453282.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11023..11775
                     /gene="gpmA"
                     /locus_tag="OQM34_002922"
     CDS             11023..11775
                     /gene="gpmA"
                     /locus_tag="OQM34_002922"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1453283.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNENILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11942..12994)
                     /gene="aroG"
                     /locus_tag="OQM34_002923"
     CDS             complement(11942..12994)
                     /gene="aroG"
                     /locus_tag="OQM34_002923"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1453284.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13310..13690
                     /locus_tag="OQM34_002924"
     CDS             13310..13690
                     /locus_tag="OQM34_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1453285.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13804..14745
                     /gene="zitB"
                     /locus_tag="OQM34_002925"
     CDS             13804..14745
                     /gene="zitB"
                     /locus_tag="OQM34_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1453286.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(14742..15461)
                     /gene="pnuC"
                     /locus_tag="OQM34_002926"
     CDS             complement(14742..15461)
                     /gene="pnuC"
                     /locus_tag="OQM34_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1453287.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15499..16542)
                     /gene="nadA"
                     /locus_tag="OQM34_002927"
     CDS             complement(15499..16542)
                     /gene="nadA"
                     /locus_tag="OQM34_002927"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1453288.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAVVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4226 a         4409 c         4164 g         3954 t
ORIGIN      
        1 agaattaatt cctaaagagc atttgcttca tgaatgctcc tttgcattta gcaactgact
       61 gtaacagcta aaattagtcg cttttggcgg tgaatgctga attaaaacgc agaattttgt
      121 ccgatagtgg tatcggtgta ccattcgcgc agacgaaaat tcttaacctt aatctggaat
      181 acgccatgac cacacgcgtg attgctctcg acttagacgg caccttattg accccgaaaa
      241 agaccttgct tccatcatcg atagaagccc tggcccgcgc tcgcgaagca ggctatcaat
      301 taatcatcgt cacaggtcgc catcacgtcg ctattcatcc tttttatcag gcgctggcgc
      361 tggatacacc tgctatttgc tgtaatggca cctatttgta tgattatcat gcaaaaaccg
      421 tgctggaagc ggacccaatg cccgttaata aagccctgca actcattgag atgctgaatg
      481 aacaccacat tcacggtctg atgtatgtcg atgatgcaat ggtctatgag cacccgaccg
      541 ggcatgtcat tcgcacgtct aactgggcgc aaaccctgcc gccggaacag cgtccgactt
      601 tcacacaagt cgcttctctg gctgaaacga cgcaacaagt taacgccgta tggaagttcg
      661 ccctgacgca cgatgacctg ccgcaattgc agcattttgg taagcatgtc gaacatgaac
      721 tgggactgga gtgtgaatgg tcctggcacg atcaggttga tattgcacac ggcggcaaca
      781 gcaaaggtaa acgtttgacg aaatgggttg aggcgcaagg ttggtcgatg gaaaacgtcg
      841 tggcattcgg cgataacttt aatgatatca gtatgctgga agccgcaggt acaggcgtgg
      901 cgatgggcaa cgccgatgac gcggtaaaag cgcgcgccaa cattgtgatt ggtgataaca
      961 ccaccgacag cattgcccag ttcatttata gccacctgat ttaatcaggc ggttatcgac
     1021 acacttttaa tttgcgcgta cagccacagg ccaggtttga tcgccagttc atccctggcc
     1081 cacgggctga tacgcgccca cagcgtttta ccgccgactt ccagctccac ttccacctgg
     1141 ccgttgtcgt cataactatt aacaactttt gcccgcaata cgttacgaat gctggtttgc
     1201 tgcggcggtt gtaaaaccag agaaacatcg gaagcctgaa tgcggatacg tagcgcagct
     1261 tgcagcggtt cgcccagctt attgacccac aaatgctgat cgcccagcgc cagcgcggtc
     1321 atcgcgtaat gcggatgatg ctcaagcacc gtcactttca gaatgctact ttgttgctct
     1381 ttcggcagcc acggattcat cacgctactg ccccacactt cctccagcgc gccaaaggct
     1441 ttcacctgac cgttttccag taccatcact ctgtctgcca gatggaggat ctcatccagc
     1501 gaatggctga catacaacat cggaatgttg atttcccgtg tcagccgttg cagataaggc
     1561 aacagttcgc gtttacgcgg aatatccagt gacgccagcg gttcatccag caacagcaat
     1621 tccggtgctg tcagcaaagc ccgaccaatc gccacgcgct gtttttcgcc tccggacagg
     1681 ctgcctggta aacggtcaag caacggttca atgcctaaaa gcgccaccag cttatcgaac
     1741 tgatcgacca tacttttcga catgccgtag cgcagattgc cacgcacttt gtaatgcggg
     1801 aacaaccgcg catcctgaaa aacatagcca acacgacgct tttccggcgt caggcagata
     1861 cctttttcgg catcatttag tacccgccca ttgaggacaa tccgcccttt ttgcgggcgc
     1921 gtcagtccac tgatggcgtt aatcagcgaa gtttttccgg caccggagac gccaaagata
     1981 gcagtgatgc cattggcggg cagcgtttca ttaatggtca ggcaatggtt gcccaacgtc
     2041 tgggaaaaat tcagctccag catgattagc gccccgcccg ttcacggctg attctggcca
     2101 gccattctga aatcaacagg gagatcatcg ccagcgcaat agaaataatg cacagtctcg
     2161 ccgcgccact ttccccgccg ggggtctgga tcagggtata catggcagaa ggaatggttc
     2221 gcgtttcgct aggaatgttc gacacaaagg tgatggttgc accaaactca ccgagagaac
     2281 gagcaaaagc cagtaccgta ccaacaataa ttcccggtaa ggtcagcggt aacgtgatag
     2341 taaagaaaac gcgccagcgc ccggcaccta gtgttcttgc ggcctgttcc agtttgacat
     2401 caaccccttc cagcgccaga cgaattgccc gcaccatcag cggaaacgac atgacggcgg
     2461 cagcgagaac cgcgccgcgc cagctaaagg cgaaggtaat accaaaccag tcatacagac
     2521 gttcaccgat aaatccgcgc cgtcccatcg aaactaataa taagtaaccg acgaccacgg
     2581 gcggtaacac cagcggtaga tgcagtacgc tgtcgagcag agctttgccc ggaaacgtgc
     2641 aacgcaccag taaccaggca aaaaagatcc caaacggcag gctaaacagc acagccaggg
     2701 aagaaacttt caggcttaat aaaactgcct gccattctgg atcggtcagt atcattactt
     2761 ggttgtaaat ccgtaacgtt taaagatttc cgctgcctgc ggtcccttca gataatcata
     2821 aaatgctttc actgtcgcat tgttatgccc ttccacaaca gcaaccggat attccacttt
     2881 tttatgtgaa tcttccggga aggtggcaac cacttttacc cctttgctgg caactgcgtc
     2941 agaaccgtag acaatgccca gaggcgcttc gttacgttcg accagcgcca gcgccccacg
     3001 aacatcttcc gctggggcca gtttcggaga gagcgtatcc catgcgccca gtttttgcag
     3061 tgcttctttt gcataaatgc cagcgggaac atgttccgga tcgccaaccg ccaggcgacc
     3121 gccattcagc agcgaagtcc agttagtttt gctgtcgatg gtgaaatctt tctgctcgct
     3181 ggctttcggt gctacaacga ccaggctatt gccgagcagt gtctgacgcg tagcagtatc
     3241 gatcgctttt ttatcaaccg cataatccat ccatttctga tcggcagaaa taaacagatc
     3301 cgcaggggca cccgcttcaa tctgacgggc gagagtagat gacgaagcga aagaagaaac
     3361 cacatccacg cctttctctt ttttatactg cgtagcaatg tcctgcattg cgttagtcag
     3421 tgatgcggcg gcgaacaccg tgattttccc ttcatctgcc agtgcattgc cagcaactgc
     3481 gaaagagagt gccgccccgg caaacaagtt caaccattta cgagccattg gtaacccctt
     3541 aatgtaacgt tatgtaggcg acaatataac gactaactcg gaattttccc agcagttatt
     3601 gctaaccttt gtgaggtaga taagaaaaaa tatcggcagg aaaagcagga agttgagaaa
     3661 aagaaaatgc ccgactaagc gggcactcag ggaatcaatg attttgtccg ggctggtctt
     3721 ttttaccaac accagaaaag atgttgaata cttcaccaag accgtaaatc agacccagga
     3781 tgatggccat cacgacaggt accatgatta cggcgaatac cagacttttt aataactcta
     3841 acatggtcaa ctccagatat agtcatgaga ctattctaac cgctaagcac agaaaagcac
     3901 tccccttttg tgcggtcagc tttgcgtggc gttcgttttc cgtcacaata agacttttgc
     3961 taggacattg ttatgcaggc cgaaatcctt ctcaccctta agctccaaca aaaattattc
     4021 gccgacccgc gccgcatttc gctactaaaa cacattgcgc tttccggttc cattagccag
     4081 ggagcgaaag atgccggtat cagctataaa agcgcctggg atgccattaa cgagatgaat
     4141 cagttaagtg agcatattct ggtcgagcgc gcaacaggcg gtaaaggtgg cggaggcgca
     4201 gtactgaccc gctatggtca gcgactgatt cagctctatg acttactggc gcaaatccag
     4261 caaaaagcct ttgatgtgtt aagtgacgat gacgccctgc cgctgaacag cctgctggcc
     4321 gcgatctcac gtttttcact gcaaaccagc gcccgtaacc agtggttcgg taccatcacc
     4381 gcccgcgatc atgatgacgt tcaacagcat gttgatgtct tactggctga cggaaaaaca
     4441 cgcctgaaag tcgcaattac cgcacaaagc ggcgcacgtc tggggctgga tgaaggcaaa
     4501 gaagtgttga tattgctaaa agcgccgtgg gtaggcatta ctcaggacga agcggtcgca
     4561 caaaacgccg acaaccaatt accgggtatt attagtcata ttgagcgcgg cgcagagcag
     4621 tgcgaagtat taatggcgct acccgacggg caaacactgt gcgccacagt gccggtaaat
     4681 gaagcgactt ctcttcagca agggcagaat gtcacggcct actttaatgc cgacagcgtg
     4741 attatcgcca cgctgtgcta agcgtgttga caatttgtta tgaaacacgt atccctgtca
     4801 gtaatcgctg cacaaaatgg gatataaaat gtcatcgttg caaattttgc aaggcacgtt
     4861 tcgtcttagc gacacaaaaa cgctgcaatt gcctcagcta acgttaaacg cgggtgatag
     4921 ttgggcgttt gtcggttcga atggaagtgg aaagtcggcg ctggcccgcg cgctggcggg
     4981 ggaacttccg cttttgaaag gtgaacggca aagccagttt tcccacatca ctcgtctctc
     5041 cttcgagcaa ttgcaaaagc tcgtcagcga cgaatggcaa cggaataaca ccgatatgct
     5101 cggccctagc gaagattaca ccggacgcac tatggctgag attattcagg atgaagtaaa
     5161 ggatgcaccg cgttgcatgc aactggcgca gcagttcggt attaccgccc tcctcgaccg
     5221 acgctttaaa tacctttcca ctggcgagac gcgaaaaacc ctgctgtgtc aggcgctgat
     5281 gtcggagcct gacttgttga ttcttgatga gccgttcgat ggcctggatg ttgcctcacg
     5341 tcagcagctg gctgagcgac tcgcctcgtt acatcagtcc ggtattactt tggtactggt
     5401 gctcaatcgc ttcgataaga tcccggaatt tgtccagttt gctggcgtgc tggcggattg
     5461 tacgttagcg gaaactggcg ctaaagagga actgctccag caagcactcg tcgcgcaact
     5521 tgcgcatagt gaacagcttg aaggtgtgca actgccggag ccggatgaac cttcagcacg
     5581 tcacgcctta cccgccaacg aatcgcgcat tgtgctgaac aatggcgtgg tttcttataa
     5641 tgatcgcccc attcttaata accttagctg gcaggtgaat ccaggcgaac actggcaaat
     5701 tgtcgggcca aatggcgcgg gaaaatcgac gttattaagc ctgattactg gcgatcatcc
     5761 gcaaggttac agcaacgatt tgacgctttt cggccgacgt cgcggcagca gcgaaaccat
     5821 ctgggacatc aaaaagcata tcggttacgt cagcagtagt ttgcatctgg attaccgggt
     5881 cagcactacc gtgcgtaatg tgattctttc tggctatttt gattcgattg gcatttatca
     5941 ggtcgtttcg gatcgccagc aaaaactggt gcagcagtgg ctggatattc tcggcattga
     6001 taaacacacg gctgacgctc cgttccatag tctttcctgg ggacagcagc gtctggcgct
     6061 gatcgtccgc gcactggtga aacatccgac gttgcttatt ctcgatgaac cactacaggg
     6121 gcttgatccg ctcaatcgcc aacttatccg ccgttttgtt gatgtgctga ttagcgaagg
     6181 tgaaacgcaa ttgttgtttg tttcgcacca cgctgaagat gcgcctgcct gtattaccca
     6241 tcgccttgag ttcgtgccgg acggtggact ctatcgctat gtgctgacaa aaatatattg
     6301 agtcggtagt gctgaccttg ccggaggcgg cctaagcgca ctctccggcc aacggttcga
     6361 cgcatgcagg catgaaaccg cttctttttt cagataaaaa gcgcaatcat tcataaaccc
     6421 tctgttttat aatcacttaa tcgcgcataa aaaacggcta aattcttgtg taaacgattc
     6481 cactaattta ttccatgtca cacttttcgc atctttgtta tgctatggtt atttcatacc
     6541 acaagcctaa tggagcgaat tatgagagtt ctggttaccg gtggtagcgg ttacattgga
     6601 agtcatacct gcgtgcaatt actgcaaaac ggtcatgatg tcatcattct tgataacctc
     6661 tgtaacagta agcgcagcgt actgcctgtt atcgagcgtt taggcggcaa acatccaacg
     6721 tttgttgaag gcgatattcg taacgaagcg ttgatgaccg agatcctgca cgatcacgct
     6781 atcgacaccg tgatccactt cgccgggctg aaagccgtcg ccgaatcggt acaaaaaccg
     6841 ctggaatatt acgacaacaa tgtcaacggc actctgcgcc tgattagcgc catgcgcgcc
     6901 gctaacgtca aaaactttat ttttagctcc tccgccaccg tttatggcga tcagccccaa
     6961 attccatacg ttgaaagctt cccgaccggc acaccgcaaa gcccttatgg caaaagcaaa
     7021 ttgatggtgg aacagatcct caccgacctg caaaaagccc agccggactg gagcattgcc
     7081 ctgctgcgct acttcaaccc ggttggcgcg catccgtcgg gcgatatggg cgaagatccg
     7141 caaggcattc cgaataacct gatgccatac atcgcccaag ttgctgtagg ccgtcgcgac
     7201 tcgctggcga tttttggtaa cgattatccg accgaagatg gtactggcgt acgcgattac
     7261 atccacgtaa tggatctggc ggacggtcac gtcgtggcga tggaaaaact ggcgaacaag
     7321 ccaggcgtac acatctacaa cctcggtgct ggcgtaggca gcagcgtgct ggacgtggtt
     7381 aatgccttca gcaaagcctg cggcaaaccg gttaactatc attttgcacc gcgtcgcgaa
     7441 ggcgaccttc cggcctactg ggcggacgcc agcaaagccg accgtgaact gaactggcgc
     7501 gtaacgcgca cactcgatga aatggcgcag gacacctggc actggcagtc acgccatcca
     7561 cagggatatc ccgattaagg aacgaccatg acgcaattta atctcgttga tcatccacat
     7621 cgccgctaca acccgctcac cgggcaatgg attctggttt caccgcaccg cgctaagcgc
     7681 ccctggcagg gggcgcagga aacgccagcc aaacaggtgt tacctgcgca cgatccagat
     7741 tgcttcctct gcgcaggtaa tgtgcgggtg acaggcgata aaaaccccga ttacaccggg
     7801 acttacgttt tcactaatga ctttgcggcc ttgatgtctg acacgccaga tgcgccagaa
     7861 agcaacgatc ctttgatgcg ttgccagagc gcgcgcggta ccagccgggt gatctgcttt
     7921 tcaccggatc acagtaaaac gttgccagaa ctgagcgttg cggcattgac ggaaatcgtc
     7981 aaaacctggc aggagcaaac cgcagagctg ggaaaaacgt acccgtgggt gcaggtcttt
     8041 gaaaacaaag gtgcggcgat gggctgctct aacccgcatc cgcacggaca gatttgggca
     8101 aatagcttcc tgcctaacga agctgagcgc gaagaccgcc tgcaaaaaga atattttgcc
     8161 gaacagaaat caccaatgct ggtggattat gttcagcgcg agctggcaga cggtagccgt
     8221 accgttgtcg aaaccgaaca ctggttagcc gttgtgcctt actgggctgc ctggccgttc
     8281 gaaacgctac tgctgcccaa agcccacgtt ttgcggatca ccgatttgac cgacgcccag
     8341 cgcagcgatt tggcgctggc gttgaaaaag ctgaccagtc gttatgacaa cctcttccag
     8401 tgctccttcc cctactctat gggctggcac ggcgcaccat ttaatggcga agagaatcaa
     8461 cactggcagc tgcacgcgca cttttatccg cctctgctgc gctccgccac cgtacgtaaa
     8521 tttatggttg gttatgaaat gctggcagag acccagcgag acctgaccgc agaacaggca
     8581 gcagagcgtt tgcgcgcagt cagcgatatc cattttcgcg aatccggagt gtaagaaatg
     8641 agtctgaaag aaaaaacaca atctctgttt gccaacgcat ttggctaccc tgccactcac
     8701 accattcagg cgcctggccg cgtgaatttg attggtgaac acaccgacta caacgacggt
     8761 ttcgttctgc cctgcgcgat tgattatcaa accgtgatca gctgtgcacc acgcgatgac
     8821 cgtaaagttc gcgtgatggc agccgattat gaaaatcagc tcgacgagtt ttccctcaat
     8881 gcgcccattg tcgcgcatga aaactatcaa tgggcgaact acgttcgtgg cgtggtgaaa
     8941 catctgcaac tgcgtaacaa cagcttcggc ggtgtggaca tggtgatcag cggcaatgtg
     9001 ccgcagggtg ccgggttaag ttcttccgct tcactggaag tcgcggtcgg aaccgtattg
     9061 cagcagcttt atcatctgcc gctggacggc gcacaaatcg cgcttaacgg tcaggaagca
     9121 gaaaaccagt ttgttggctg taactgcggg atcatggatc agctaatttc cgcactcggc
     9181 aagaaagatc atgccttgct gattgactgc cgctcactgg ggactaaagc agtttccatg
     9241 ccgaaaggtg tggctgtcgt catcatcaac agtaacttca aacgtaccct ggttggcagc
     9301 gaatacaaca cccgtcgtga acagtgcgaa accggtgcgc gtttcttcca gcagccagct
     9361 ctgcgcgatg tcaccattga agagttcaac gctgttgcac atgagctgga cccaatcgtg
     9421 gcgaaacgcg tgcgtcatat cctgactgaa aacgcccgca ccgttgaagc tgccagcgcg
     9481 ctggagcaag gcgacctgaa acgtatgggc gagttgatgg ccgagtctca tgcctctatg
     9541 cgcgatgatt tcgaaatcac cgtgccgcaa attgacactc tggtagaaat cgtcaaagct
     9601 gtgattggcg acaaaggtgg cgtacgcatg accggcggcg gatttggcgg ctgtatcgtc
     9661 gcgctgatcc cggaagagct ggtgcctgcc gtacagcaag ctgtcgctga acaatatgaa
     9721 gcaaaaacag gtattaaaga gactttttac gtttgtaaac catcacaagg agcaggacag
     9781 tgctgaacga aactcccgca ctggcacccg atggtcagcc gtaccgactg ttaactttgc
     9841 gtaacaacgc agggatggta gtcacgctga tggactgggg tgcgacttta ctttccgccc
     9901 gtattccgct ttccgatggc agcgtccgcg aggcgctgct cggctgtgcc agtccggaat
     9961 gctatcagga tcaggctgcg tttctggggg cctctattgg tcgttatgcc aaccgcatcg
    10021 ccaatagtcg ctataccttt gacggtgaaa ccgtgacgct ttcgccaagc cagggcgtta
    10081 accagctgca cggcggcccg gaagggttcg acaaacgtcg ctggcagatt gtgaaccaga
    10141 acgatcgtca ggtgctgttt gccctgagtt cagatgatgg cgatcagggc ttcccgggta
    10201 atctcggcgc gacggtgcaa tatcgtctga ccgacgataa ccgtatctcc attacttatc
    10261 gcgccacagt tgataaacct tgcccggtga atatgactaa tcacgtctat ttcaatcttg
    10321 acggcgagca gtctgacgtg cgcaatcaca agttgcagat tctggcggac gaatatcttc
    10381 cggttgatga aggcggcatt ccgcacgacg gcttgaaatc tgtcgccgga acgtcttttg
    10441 atttccgcag cgccaaaatc atcgccagtg agtttcttgc cgacgacgat cagcgcaaag
    10501 tgaaaggtta cgatcacgca ttcttgttac aggccaaagg cgatggcaag aaagtggcgg
    10561 cgcatgtctg gtcagcagat gaaaaattgc agctgaaggt ctacaccacc gctccggctc
    10621 ttcaattcta ctccggcaac ttcctcggcg gcacaccgtc gcggggaacc gaaccttacg
    10681 ccgactggca agggctggcg ttggaaagcg agtttctgcc ggacagcccg aaccaccctg
    10741 aatggccgca accggactgc ttcctgcgtc ctggcgaaga gtattccagc ctgacggaat
    10801 atcagtttat tgctgagtaa tgttaagccc tcgtcatgag ggctttatct tcatattgtt
    10861 caaatcacca gcaaacaccg acatatttgc aactcaatat tcacaacaac cttacactgc
    10921 gccactattt tcgctatggt tatgcgtaag cattgctgtt gcttcgtcac ggcaatataa
    10981 tgagaattat tatcattaaa agatgatttg aggagtaagc atatggctgt aactaagctg
    11041 gttctggttc gtcatggcga aagtcagtgg aacaaagaaa accgtttcac cggttggtac
    11101 gacgtggatc tgtctgagaa aggcgtaagc gaagcaaaag cagcaggtaa gctgctgaaa
    11161 gaggaaggtt acagctttga ctttgcttac acttctgtgc tgaaacgcgc tatccatacc
    11221 ctgtggaatg tgctggacga actggatcag gcatggctgc ccgttgagaa atcctggaaa
    11281 ctgaacgaac gtcactacgg tgcgttgcag ggtctgaaca aagcggaaac tgctgaaaag
    11341 tatggcgacg agcaggtgaa acagtggcgt cgtggttttg cagtgactcc gccggaactg
    11401 actaaagatg atgagcgtta tccgggtcac gatccgcgtt acgcgaaact gagcgagaaa
    11461 gaattgccgc tgacggaaag cctggcgctg accattgacc gcgtgatccc ttactggaat
    11521 gaaaacattc tgccgcgtat gaagagcggt gagcgcgtaa tcatcgccgc acacggtaac
    11581 tccctgcgtg cgctggtgaa atatcttgat aacatgagcg aagaagagat tcttgagctg
    11641 aatatcccga ctggcgtgcc gctggtgtat gagttcgacg agaatttcaa accgctgaaa
    11701 cgctattatc tgggtaatgc tgacgagatc gcggcgaaag cagcagccgt tgcgaaccag
    11761 ggtaaagcga agtaaacgtc attcgtttaa aaaatgagaa agccgactgc aagtgagtcg
    11821 gcttttttgt ttgtcgttgc taacaatgga gcacattgcc tgatgcgacg ctgcgcgtct
    11881 tatcaggcct gtggtgattc atcggatacg ccactctgac ggcgcatccg acaattaaac
    11941 cttacccgcg acgcgctttt actgcattca ccagttgacg taacagagca tcggtatctt
    12001 cccagccaat gcaggcatcg gtgatgctct taccgtaggc cagcggctcc ccgctatcga
    12061 ggctctgatt gccttccacc agatggcttt ccaccatcac gccaataatg gccttttcgc
    12121 caccggcaat ctgctggcaa acgtcagcac aaacatccat ctgctttttg aattgtttgg
    12181 acgaattagc atggctgaaa tcgatcatca cctgagctgg cagacctgct ttgttcagcc
    12241 cttctttcac ttcagcaacg tgcttcgcgc tgtagttagg ctctttaccg ccgcgcagaa
    12301 tgatatggca atcgccgtta ccactggtat tcacaatcgc cgaatgcccc catttagtta
    12361 cggacaggaa gcagtgcggc gcaccggcgg cattaatggc atcgatagcc actttaatcg
    12421 taccgtctgt gccattttta aaacctaccg gacaagaaag accagacgcc agttcgcggt
    12481 gcacctgcga ttccgtggta cgtgcaccaa ttgcgcccca gctcatcagg tcagcgagat
    12541 actgaggagt gatcatatcg agaaactcac ccgccgctgg cagaccgctg tcgttaatat
    12601 caagcagcaa tttacgggct atacgcagac cgtcgttgat ctggaagctg ttatccatat
    12661 gcggatcgtt aatcaaccct ttccagccca ccgtggtacg cggcttttca aaatagacgc
    12721 gcattacgat ttccagctca tctttcagct cttcacgcag cgccagcaag cgagtggcat
    12781 actctttagc cgcgacagga tcatgaattg agcacgggcc aatcaccacc aacaggcgat
    12841 catcattacc tttcaggatc ttatggatcg cttttcgggc atgggcaacc gtattcgcgg
    12901 cattttcagt tgcggggaat ttttccagca atgcgacagg aggaagtaac tctttgattt
    12961 ctttgatgcg taaatcgtcg ttctgataat tcatgtctgt tccagtgttg ccatacttat
    13021 cttagtgaat gcaatacttc caatctatat cttccgtcag aatgtgtaaa cggggtttta
    13081 cactatgaac ggattaatcc tggaattagc aagaaaaacg ccagattgtc gcgaaaaacg
    13141 agatctctcc tacaatttct aactgtaact cctttgtttg ttaattattt caagattctc
    13201 tgctgcgttt cataaactgg ctgaaatctt aaaccaatgc cttatattca cctgcaaatg
    13261 cactgttgga agaggttatc cgacataacg accataacag gagcatccta tgaaaatgac
    13321 aaaactggcc acattatttc tgactgccac tctaagcctt gccagcggtg ccgcactggc
    13381 tgccgatagc ggagcgcaaa ctaataacgg ccaggcaaac gccgcagctg atgcgggcca
    13441 ggtagcccct gacgcccgtg aaaatgtcgc gccaaataac gtcgacaata acggggtaaa
    13501 taccggttct ggcggcacaa tgctgcattc ggatggttct tcaatgaaca atgacggaat
    13561 gaccaaagat gaagagcaca agaacaccat gtgcaaagat ggtcgatgtc cggatatcaa
    13621 taaaaaggta caaaccggtg atggcatcaa taatgatgtc gataccaaaa ccgacggtac
    13681 cacgcagtaa ccctgcaaaa tagccaataa tgggagagcg gagtctctcc cttttaattt
    13741 ttcacttcta aaagcagtat gatgtcataa agttaagtga gaataattaa gggatgactc
    13801 gtcatggcgc actcacactc acacacgtcc tcacacctgc cagaagataa taatgctcgt
    13861 cgcttgttgt atgctttcgg cgtgactgcc gggtttatgc tggtagaagt cgttggtggt
    13921 tttctttctg gttctctggc attgctggcc gatgcgggtc atatgttgac cgatactgcc
    13981 gccctgcttt tcgccctact cgccgtgcaa ttttcacgtc gtcctcccac cattcgccac
    14041 actttcggtt ggctaagact gaccacgctc gcggcgtttg tgaatgccat cgccctcgtg
    14101 gtaattacca ttttgattgt ctgggaggcg atagaacgct tccgcacgcc gcgtccggtc
    14161 gagggaggca tgatgatggc aattgccgtg gctgggttgc tggcaaatat actttctttc
    14221 tggttacttc atcacggcag cgaagagaaa aacctcaacg tacgagcagc ggcactgcat
    14281 gtgctcgggg acctgctggg ttcggttggg gctattattg ccgcgttgat tattatctgg
    14341 actggctgga cacctgctga ccccattctc tcgatactgg tgtcgcttct ggttctgcgt
    14401 agcgcgtggc gactgttgaa agatagcgtg aatgaattac ttgaaggtgc accggtatcg
    14461 ctggatatcg ctgaactgaa gcgtcgtatg tgccgggaaa tcccggaagt ccgcaatgtg
    14521 caccatgtac atgtatggat ggtaggcgag aagccggtga tgacgttgca tgtgcaggtg
    14581 atcccgccac acgatcacga tgccttgttg gatcagatcc aacactacct gatggatcac
    14641 tatcagattg agcacaccac cattcagatg gaatatcaac cttgtcatag gccagactgc
    14701 cacctgaatg aaggggtgtc tggccattca catcatcacc attaatggga cagcgcgcgt
    14761 gagcctcttt cacgtgcgct gttgatccac atccggctgc cgttgagcgc aataaaggtc
    14821 aggatgatgt actccagaga catggcgtaa acgccctgaa gtgcaaaaat aacgacgcta
    14881 atcacgttaa taatcaccca caacagccag ttttccacat acttacgcgt catcagaatc
    14941 attgccacga tagataacac catcatgcat gaatcccaga acgggaaagc gtccggttgc
    15001 agttcaggca tcacaacctg taatcctaat gcttgcatga tcatgactgc cacgcgggtc
    15061 aaaaatgcaa acaccggatt gataaatacc gtcatcagac caatcgaaac aacgcaaacc
    15121 gccaaccagc tgagtgcctt cggcaatggc aaccagcgaa ttttcaactc cgcctcgttc
    15181 tgactggttt gtcgcgacca cgcataccag ccgtaaatat tcgcggcaaa gaaaaacacc
    15241 tgtaatagca ggctggcata cagctgaatc tgaaagaaaa taatgccaaa caaggtaacg
    15301 ttgatcaggc caaagaagta gttgctgatc ttctccagac tggcaaggcc aatacacagc
    15361 aacccggcga tcgtgcctac cgcttcgatc catgagagat cataaccgcc cgcccctatt
    15421 ggtatatgta ccaggatatt ctgcacacta aaaaaatcca tctttccccc aaagcgtaac
    15481 gttacgcctt attattcgtt atccacgtag tgtagccgca aaatccagca tacgattgag
    15541 cggcaccagc gccctctctc gcagcctctc atcgacatga acctcgtgat tacttccttc
    15601 ctgttctaat gcctctgcga tagcctgaag gtcattcatg gccatccacg gacaatgcgc
    15661 gcagctgcgg caggttgcgc cctcacccgc ggttggtgct tccagtaact ctttatctgg
    15721 caccgcctgc tgcattttgt agaaaatacc ccgatcggtt gccacaataa gcctctgatg
    15781 aggcaatgct tttgcagcag cgatcagttg actggtggaa ccgaccgcat ccgccatatc
    15841 gacaatagct tgtggtgatt ctggatgcac cagtatggca gcatccgggt attcttcttg
    15901 caagcgggtt aacgcctgag tcttaaattc atcatgcaca atgcaggcac cctgccagca
    15961 taaaatgtct ccacccgtct gtttttgcac gtaacgcccc agatgtttgt cgggtgccca
    16021 gatgattttt tcacccaaac tatcaagatg atcaataagt tcgacggcaa tgcttgaagt
    16081 taccacccaa tctgcgcgcg cttttaccac agcagaagtg ttggcgtaga cgacgacagt
    16141 acgatcggga tgggcatcgt aaaatgcgtt aaattcttca acagggcagc cgagatccag
    16201 tgaacactca acctgaagtg tcggcatcag aattgttttt tccggactga gaattttggc
    16261 ggtttctccc ataaatctca ccccagcgac taacaaagta gaagcgggat gctttgcacc
    16321 gaagcgcgcc atttccagag aatcagaaat acagccaccg gtttcttctg ccagttgttg
    16381 aatttcggga tcggtatagt agtgggcaac catcaccgca ttacgttctt ttagcagacg
    16441 ttttatcttc tcgcggtaat acgctttttc atcaatgctt aacggcgtcg gcttcggggg
    16501 gaaaggataa atcgccgtgt ctggatcaaa cattacgctc atcttaccat ctcgttttac
    16561 aggcttaacg ttaaaaccga cattagcgta acattcgctg ttatcggtgt aatttgtttt
    16621 atatgctaaa caagatagtc gaatggtggg gaaaagtcac tcgaattacg cctaattcca
    16681 ccgacgtaga gttgcttgca cagtaagctc tgacgatgtc atcaggttta caaagtgaaa
    16741 ttgaggatgc ctg
//
LOCUS       DAJVPP010000086        16545 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVPP010000086 DAJVPP010000000
VERSION     DAJVPP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.086"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(31..222)
                     /locus_tag="OQM34_002928"
     CDS             complement(31..222)
                     /locus_tag="OQM34_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCS1453289.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(327..563)
                     /locus_tag="OQM34_002929"
     CDS             complement(327..563)
                     /locus_tag="OQM34_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCS1453290.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(681..2120)
                     /gene="rsmF"
                     /locus_tag="OQM34_002930"
     CDS             complement(681..2120)
                     /gene="rsmF"
                     /locus_tag="OQM34_002930"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS1453291.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2200..4820)
                     /gene="yebT"
                     /locus_tag="OQM34_002931"
                     /pseudo
     CDS             complement(2200..4820)
                     /gene="yebT"
                     /locus_tag="OQM34_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4789..6072)
                     /gene="yebS"
                     /locus_tag="OQM34_002932"
                     /pseudo
     CDS             complement(4789..6072)
                     /gene="yebS"
                     /locus_tag="OQM34_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6202..6699
                     /gene="msrC"
                     /locus_tag="OQM34_002933"
     CDS             6202..6699
                     /gene="msrC"
                     /locus_tag="OQM34_002933"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1453292.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6796..7494
                     /gene="proQ"
                     /locus_tag="OQM34_002934"
     CDS             6796..7494
                     /gene="proQ"
                     /locus_tag="OQM34_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1453293.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7514..9562
                     /gene="prc"
                     /locus_tag="OQM34_002935"
     CDS             7514..9562
                     /gene="prc"
                     /locus_tag="OQM34_002935"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1453294.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9754..10635
                     /gene="htpX"
                     /locus_tag="OQM34_002936"
     CDS             9754..10635
                     /gene="htpX"
                     /locus_tag="OQM34_002936"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1453295.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10681..12054)
                     /locus_tag="OQM34_002937"
     CDS             complement(10681..12054)
                     /locus_tag="OQM34_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1453296.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12231..13022
                     /gene="kdgR"
                     /locus_tag="OQM34_002938"
     CDS             12231..13022
                     /gene="kdgR"
                     /locus_tag="OQM34_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1453297.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13165..13404)
                     /locus_tag="OQM34_002939"
     CDS             complement(13165..13404)
                     /locus_tag="OQM34_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453298.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13563..13706
                     /gene="mgrB"
                     /locus_tag="OQM34_002940"
     CDS             13563..13706
                     /gene="mgrB"
                     /locus_tag="OQM34_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1453299.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13781..14068
                     /locus_tag="OQM34_002941"
     CDS             13781..14068
                     /locus_tag="OQM34_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1453300.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14738..14881
                     /locus_tag="OQM34_002942"
     CDS             14738..14881
                     /locus_tag="OQM34_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1453301.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14894..15103
                     /gene="cspE"
                     /locus_tag="OQM34_002943"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14894..15103
                     /gene="cspE"
                     /locus_tag="OQM34_002943"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1453302.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15269..16078
                     /gene="rlmA"
                     /locus_tag="OQM34_002944"
     CDS             15269..16078
                     /gene="rlmA"
                     /locus_tag="OQM34_002944"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1453303.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16075..>16545)
                     /gene="mntP"
                     /locus_tag="OQM34_002945"
     CDS             complement(16075..>16545)
                     /gene="mntP"
                     /locus_tag="OQM34_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1453304.1"
                     /translation="FSEALRTGLIFGAVETLTPLIGWGMGMLASRFVLEWNHWIAFVL
                     LIFLGGRMIIEGFRGADDEDEEPRRRHGFWLLVTTAIATSLDAMAVGVGLAFLQVNII
                     ATALAIGCATLIMSTLGMMVGRFIGSIIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4342 a         4025 c         4196 g         3982 t
ORIGIN      
        1 ggctaactga ctcttgtgca actgcaataa ttaaaaatca ccatactcct gtgctggtcg
       61 ccagaaacca tctataaaat cctcaatcgg aaaacaaccg ccatggcgga tccgttgatc
      121 gctcatagaa taaagacact gctgttccgt gttgtagaca tccacaacaa tatcttcaca
      181 accgccatct aggtagcaaa caaaaagtac cagcgcgaac atttcatccc cgaagtgtgg
      241 tgccgtaccg ttaagtttag gagagatttt acaacggggg aataaccagg acaaataacc
      301 cgccatgccg acgggttctt tttggatcag gcaagacgca taatccaggc atcagattgt
      361 tgatcgtaac gggtacggta aaggacagag tgttcgccat ttagcagggc agggatactg
      421 gtttcagcat cccaggcatc cagttgcata cataaatcgg ggtcagattt acaaggaatg
      481 gttaacgttc gatcacccgg cgattcaccg tgtaattcgc cgtcgtcgat ttcaaaagcg
      541 cctatgcgca cactggtttt catcttcgtg ctctccattt agctgctgat tgtagtacga
      601 ccagtccgat cgcacaattt ttcagcctag ttgaggccgg cgaatgtgcc agtcaaaaag
      661 tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa gtttcccgtc ccgcaccagt
      721 tcgcgcgggt agctgttttt cagtcgcgaa ccaatccgtt tggctaaacc aatcggctga
      781 tgctggaaag taaccaacac gtcatccgcc actggcgcgg cttgcgggta aacatcgcgc
      841 ccgcgatacc actcctccgc ttcctgcggt gtcagttcaa aagcgtttac attgtcgggg
      901 gaggcatggg caataaccgc ttcatgctgc cagcgataac ctttgttatg cgtttcggca
      961 agtttaatcc ctaagcgaga aaatcggact ttaccgatca gggcttcaat gcctaccggg
     1021 aacaaccaca gttctttgtc gcgttgccag agtcgcaggt ttccatccca gtttaagcca
     1081 acacttgcag ccgcctgacg aatttgtccg gcttcgcgat ctttcaccgg gctgaacggg
     1141 aaattgccca ctttgtattt tggggcgggt aaggcgggga tcgcctgagt tttacgcaga
     1201 cgagcaacga agaagccttc gcagtcgtaa atttgtggga aaacatgcaa aaaaccttct
     1261 tcggtcagcg ccttgtttgc accagggaag agatcgccaa gtggtaaaaa ctctacttcg
     1321 tcggggtaag tctctttcag ccaccggcaa acggcttcgt tttcttcccg gtttaaggta
     1381 caggtcgagt aaaccagcgt accgccagga cgtaatgcat gaaaggcgct gtcaatcagt
     1441 tcccgttggg tagctgcgat ttcctgattg ctttcgggtg accagttttt tagcgcatcg
     1501 ggatctttac gcaccacgcc ttcgccggag cagggagcgt ccagcaaaat ggcatcaaac
     1561 atttctggca ctgccgcacc aaacacgcgg ccaccaaaat gtgtgagcgc aacattactg
     1621 atgccacagc ggctgatatt ggcatgtaac acttttaccc aactggcgga aaactcattg
     1681 gcaaggattg ccccttcgtt attcatccgc gcggcaattt gcgtcgtttt tgagcctggc
     1741 gcagcagcga catccatcac ccgctgtggt gcattaccgt cagcaaacaa ggcggcgacg
     1801 ggcaacattg aactggcttc ctgaatataa aacaggccac ttaaatgctc ggtggtacta
     1861 cccaatggca atgcatcttc attgtcgcgt tcaatccaga aaccttcttc acaccacgga
     1921 attggcgtaa gcgtccagcc ataaggagcg gttaattgca ggaaatcagc aacggagatt
     1981 ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct gacaggcggc aagaaaatca
     2041 tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg tcagaaacgc gtccgggaaa
     2101 taaacggtgt gttgggccac gagcatgtac cactggcaaa aaaacaggcg cgcagtttag
     2161 cataagcgct ccggcgcagg cacgccggag cagtgggcat tatttgggaa gcgcagttcc
     2221 ccattcacgc cactctttcg gttcactttc ctgcaacagg aagtgtttgc cttcttgggc
     2281 tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg atgccgccac ggataaattg
     2341 gttaaaggtg ctggttttca ctacgccgcc cgtcagacca aagtccagac tgtaacccga
     2401 tgccaaccag aagacggaat tgttacgcac caggtgttga tagcgtttac tgatgcgcat
     2461 cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt ccggtaaccg tacctacttc
     2521 cagaccacgg aacagcacag gcgtaccgat gcctaacgaa ccggcttccg gcgcttcaac
     2581 aataatgctt aaaccatcta ggtaacgcga atcagtaatg gtggcctctt ctaattcaaa
     2641 gtcgcgacga ggattgccac ggcctggttc gacgttgata tacggctgga ggatagtatc
     2701 tagatgctca acgccagctg ccgagatttg cggtgtgacc actgagaagc gcgtaccacc
     2761 gcgagcaaag gtctggacat attccggata gagcaccgcc tttgcctgta cttcattgcg
     2821 tgcggtaatc agatccagcg tctggatttg cccgatatca ataccgagat agcgaattgg
     2881 catcccgacc gccagttttc cggcatcgaa agcgtgaagc gtaatctgcc tgccaaccgc
     2941 acgggccgct gtttcggaag catacagaat tcgcttgtcg cctttacgct gactggcgct
     3001 ggcaccactg aggttatcga agctaatggc cccctttaat gctctggaga gcggggatgc
     3061 ctgtacggtc aggccactac cattcagctg aactttcgcc ccgccttctg cccagaacac
     3121 gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg tgcagatcga tatcgaacgc
     3181 gttagctcgc ggacgcacgg taataacttc accaacttca aatttacggt acagcactac
     3241 cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa ctcacggttg tggtgggtaa
     3301 atcgctaagg ctgttctcca gcgctttttc cagattggca tacagtggat agctggcttt
     3361 catctcgcct ttatcgcccg gcagaatacg tatcccgccg ttaatccatt ctgaggcgct
     3421 ggcaccgaga aactcaacgc catccagccc caccttcacg tcgacacggc tgttgacgac
     3481 aaatttgcta tcgcctttta ccagttctcg atgctgaggc tcgatggcga cggtaaaggt
     3541 gacgcctttg ctggtgagtt tacgatcgat aacctggcct acctgcacgc cgtgaagaat
     3601 gagcggctga cccgcatcaa taccgtaact ttccggtgct gtcagggtca gtgtcagaac
     3661 gtccggctct tgcagcaggg ctttttcgcc cggcacaaca acgaactctt tgcgtggctc
     3721 gccatcgccg ggcaccaact caaaggtttt tccggtcagc agggcgctga gattagcatc
     3781 gctaagggat aatttcgggt tgcgtaattc gatgcgggta ttttcacgaa gcagggatca
     3841 acggtcattt ccccagtgac tttaccacca ggatttaaat ccagtttagt cagctgtccg
     3901 acttccagcc cctgatacat taacggcgtc gagtcggcgg ttaatcctgc tccactcggc
     3961 agttccagtt ttattattac tccacgctgg ctgtgggcca gatcttcata cagaccaaag
     4021 gtatcttccg cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta
     4081 accagtgccg ccagactttc cagtttcacc ttcgcgccac tgatactgac gttggcatca
     4141 acgccggaaa cgtttcagaa acggctacct tttttcacca gatcggtaaa acgccgctcg
     4201 atcaggacat caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt
     4261 cccaccggga tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagagcgggg
     4321 gcttgcaggt ggatcatcag atcgccattg tccagccgat atttcggttg ggtatcgagc
     4381 gcgacaaagt gatcctgctc tttaccttta cccggcatca tgccgatata gttcccaccg
     4441 acgagggcgt ccagcccgga gacacctgcc aacgatgctt ttggcgtcat cagccagaac
     4501 tgagtctctt cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc
     4561 ttacgaagat cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa
     4621 cgaacaggcg tacggcccgg aacaatacca tccgccgaca taaagtcgat ggtgacggta
     4681 ttaccccggt cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa
     4741 ggcagcagcc agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa
     4801 gcgggcgttt cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc
     4861 aagaatagtc aaaattacgg cagcgccaaa ataaaacgca gccggtccca tagtaaaagc
     4921 gaggatctga tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat
     4981 cgaccagcgt ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg
     5041 ctggcattta aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg
     5101 taccagaata ctggcgataa acacgattcc cgcaaccgca atgttgctac ttgccagcga
     5161 cataattccg gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat
     5221 aggcaatagg ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt
     5281 ttgcaaacta tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agagaccacg
     5341 ctgatcagga tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg
     5401 cgtagcgggg cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa
     5461 cgtcaccgtc gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctagat
     5521 atgggcataa tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag
     5581 cattacccac tctttaagtc gctcaagcat cagcagtacc ggacgtaaat tcattcccag
     5641 tcggttacca aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac
     5701 gcagaaaaag accattgatc ccgttatcgc atcgccctgt ttggtcattt gccagatgcc
     5761 ttgcatgacg ttggcgtcaa tacgaatgcc taacagccag atatgcaaca gcggttcgcc
     5821 ccaggcaaac ggcataagca acagcatggt gaaggccatt gccgccaggc gcgttagcga
     5881 ccagtcgcgc ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tttgatgaga
     5941 atttatctcg ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg
     6001 cggcagttcc tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt
     6061 gttaagagcc atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg
     6121 gaatgatctt gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta
     6181 agacaaccaa gtgattaagt aatgaacaaa acagaatttt acgcggattt aaatcgcgac
     6241 tttaacgcgc tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg
     6301 ttgttatacg agcgtctcac tgacgttaac tgggcaggtt tttatttgct tgaggacgat
     6361 acactggtac tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc
     6421 ggggtgtgcg gcacagcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg
     6481 tttgacgggc atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg
     6541 gtgaaaaatc agattattgg tgttctcgac atcgatagta ccgtcttcgc tcgctttaca
     6601 gacgaggacg agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg
     6661 acggattaca aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt
     6721 actggtagcg tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg
     6781 taatcaggaa atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg
     6841 tttctggccg aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa
     6901 attggtattt ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg
     6961 caattacgat ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa
     7021 cccggcgcaa cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat
     7081 gtagagcatg ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct
     7141 gaacagcaag cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcaccg
     7201 cgccgcgaac gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa
     7261 cctcgtgcgc aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa
     7321 cagcacaccc cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa
     7381 gcgggtcaaa acgcgatgga tgccaccgta ttagaaatca ccaaagacgg cgtccgcgtc
     7441 cagctgaatt cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga
     7501 ggccgggcca ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat
     7561 agcaggccag accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa
     7621 ggaagagacg cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca
     7681 ttatcgccag ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa
     7741 tctgctcgat tacagccaca atgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa
     7801 gaaaaccgag ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta
     7861 caatctggcg caaaagcgcc gttttgaacg ttaccagtac gctttgtcgg tactggaaaa
     7921 gccgatggat ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc
     7981 gaaaaacgag gctgagttga acgcgcagtg ggacagtaaa gtcaaattcg acgagttaag
     8041 cctgaagctg acaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa
     8101 atttgccatt cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac
     8161 ggcgtttgcg cgtgaaatcg atccgcatac caactatctt tccccgcgta ataccgaaca
     8221 gttcaacact gaaatgagtt tgtcactgga aggtattggc gcagtgctgc aaatggatga
     8281 tgactacacc gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat
     8341 cagcgttggt gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgatgtgat
     8401 tggctggcgt cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg
     8461 tctggaaatt ttacctgctg gtaaagggac caagacccgt actgtaacgt tgacccgtga
     8521 acgtattcgt ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa
     8581 agtcggcgtg ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca
     8641 actgcagaaa ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg
     8701 cggtggggcg ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat
     8761 tgttcaggtc cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt
     8821 gttctataaa ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat
     8881 ctttgccgcg gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg
     8941 taaaggcacc gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc
     9001 tgaatggcca gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg
     9061 cggcagtacg caacgtaaag gcgtaacacc agacatcatc atgccgacgg gtaatgaaga
     9121 aacggaaacg ggtgagaaat tcgaagataa cgcgctgccg tgggatagca tcgatgccgc
     9181 gacttatgtg aaatcaggag atttaactgc ctttgaaccg gagctgctga aggaacataa
     9241 tgcgcgtatc gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa
     9301 cgctatgaag gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa
     9361 taatgaagat gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gagaaggtaa
     9421 accggagttg aagaagctgg atgatctatc gaaagattac caggagccgg atccttatct
     9481 ggatgagacg gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga
     9541 acaacccgct cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa
     9601 cagcggcaag atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac
     9661 ttacgcacta tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat
     9721 tgcgttttgt taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta
     9781 acgaacctgg ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg
     9841 agcagcgttc aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt
     9901 tcgcttctga tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa
     9961 ccgcgtaacg aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggcg
    10021 gggatcgcta tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc
    10081 ggtgcgcgcc gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc
    10141 ccggatgaag ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg
    10201 gtcaccatga cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt
    10261 ctggcgcagc ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac
    10321 ggcaacccgc tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg
    10381 gcgagcatta tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg
    10441 gcaaaactgg ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat
    10501 gaaccgcaag aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc
    10561 agtgagttgt tcatgactca cccgccgctg gataaacgta ttgaagctct gcgtacgggt
    10621 gaatacctga agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt
    10681 ttatgccctg gctcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc
    10741 cagaatagcc gcagccatca gcgagacgtg cgtaccatta tcaccaaact gatttagcat
    10801 cagcgccacc agcgccgcgc cactactctg acccagtaga cgagccgttc ctaacatgcc
    10861 actggcccca ccgctacgtt cgcgcggagc ggaggtaata atggtgtggt tattgggtga
    10921 ctggaataag ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc
    10981 aggtgacgcg ggcagcagaa ccagggaaaa aagccccgta gccatgatga acaaccctaa
    11041 agcacccagc aatcctgcat gtacgcgttc aatcaaatag cctgccagcg gagccatcac
    11101 catcgttgct aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag
    11161 cacggtttgc aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca
    11221 aacagatgtg caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag
    11281 cggtacggga agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac
    11341 cagttccgca gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat
    11401 aagcaggccg aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact
    11461 ggcgcgagaa ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa
    11521 cggtacgttg attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat
    11581 tgtcggcccg gcagcagaag agacggcaac aataaacgag tttatgccca tccctctccc
    11641 cagaaaacgt tgtggataga tcaggcggat aagtgcggta ttaacgctca tcaacgctgc
    11701 accgccgaaa ccttgtatga cacgcgcaag ggtgagcatt tgcagcgaat cagaaagggc
    11761 gcagaacagt gaagacaaca gaaaaacgac cagaccgcat ttataaatac gtcgatagcc
    11821 aaacatatcg cccagaaacg aaaacgagag cagggagatg acaatggcga tttgataggc
    11881 gttcactacc cagatggaac tggctggtgt ggcatgaagg tccgtggcaa ttgttggcag
    11941 ggcgacgttg gcgattgcgc cgtcaaggac agccatcgaa ataccaatca caatggttaa
    12001 tatcgcaccg tatcgctggg gcaatggcag gccgtcggcc tgaacttttg gcataggaat
    12061 tttatatctt tggtgaataa tcaacagggg tatgattcta gcactgatat tcacggtgga
    12121 tgtcgcagaa ttgtaactaa ccggcaaaag atggattgcg accaccctgg tgcgaaatta
    12181 taataaaaaa cagttctgat ttttataaaa cactcgcaat gaggtgataa atggctaacg
    12241 cagatctgga taaacagcct gattctgtat cttccgtgct aaaagttttt ggcattttgc
    12301 aggcgctggg tgaagagcgc gaaataggga taaccgagct gtcgcagcgc gtcatgatgt
    12361 caaaaagcac cgtttatcgc tttttacaga ccatgaaaac cttaggttat gtggcgcagg
    12421 aaggggagtc ggagaaatat tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt
    12481 tacaaaacgt cgatttaatt cgtagcgcag atatccagat gcgtgagatc tcccgcctga
    12541 ccaaagaaac tatccacctc ggcgcactgg acgaagacag tattgtttac attcacaaaa
    12601 ttgactctat gtacaatttg cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca
    12661 gcaccgcgat tggtaaggta ctgctggcat ggcgcgatcg cgatgaagtg aagcaaattc
    12721 ttgagggcgt ggagtataaa cgcagtaccg agcggaccat caccagtaca gaagcgttat
    12781 tacccgttct ggaccaggtg cgcgagcagg ggtatggaga agataacgaa gagcaggaag
    12841 aagggctgca atgcattgcg gtaccggtat ttgatcgctt tggcgtggtc attgccggtt
    12901 tgagcatctc cttcccgacg ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa
    12961 tgttgcatac cgcagcgcgc aaaatttctg cccaaatggg ttatcacgac tatccgttct
    13021 gatgagagta agaacctgtc ggaatatcaa acagacaggt tctttattta gcatgagaaa
    13081 aataaagttg aaggtgcgtt atattaaacg cgcttgctat aagaatattt tactcatgag
    13141 tgagaatctg gttatttatt acccttaacc attatcgacc acgatattgc ttttacgtaa
    13201 cagtgggcaa tctgttatct ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct
    13261 ggtgcctctg gcagtcagat aggtacattg caaacctaat cctgcggcat tctctttgct
    13321 tccaatcaaa acgccatatc cgctgagtaa taatcctatc cataccagtg ctatcagcat
    13381 aactgtgcga atgatgaatc gcattacaat ctcttctctt tttatgttcg cttaatcgta
    13441 gcggcaatat gcgctgaagc aagctactca ttccgaaaaa gcacgaatat cgacatagtt
    13501 aggcgctgtt taactaacgc atgctagttt aatgacataa ggtaggtgaa gcggagattg
    13561 gagtgaaaaa gtttcgatgg gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt
    13621 gggcgcaggt attcaacatg atgtgcgatc aggatgtaca atttttcagc ggaatttgtg
    13681 ccattaacca gtttatcccg tggtgatatc attttttcag cactagattt tcttcctctc
    13741 cagcagtggt agaatagcgc ctctactcag aggtggtgaa atgaacgaag ttgtaaattc
    13801 aggcgtgatg aacattgcgt ctttggttgt atcggtggtg gttcttctta tcgggctcat
    13861 cttgtggttt tttatcaatc gtgccagttc acggactaac gaacagattg aactgcttga
    13921 ggcgttgctg gatcagcaaa aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa
    13981 cgaaccagag aaagcagata aaaagaccat tgagagtcaa aaatcggttg atgacgaaga
    14041 tattattcgc ctggtcgccg aacgataatc cttcctcaat cttgagtaac cccgtcgttg
    14101 aagggttact caattcatcc tgattatacg cactgtcatg gtgcaactgc ttcaaattaa
    14161 aactctggtc tgacttcaaa taattatcat attatttatt tgcgtaacgg gatatcagaa
    14221 aaagatattt tcctcattaa tttattagga tgtttacatc agatttgtga ttaagcgtgg
    14281 tattatttat tagcgaaacg tttctctctt gatttttttg ctcactcatc aatttttctt
    14341 attttaaatt tacaatccct ttggggattg acttctcttt agggtaattg atagccgtta
    14401 actgactgtt ttatgagaaa aagtgatata actttttatt cattgcatag cagaaaatgt
    14461 gatattgcac gcactatgta ataacttctc cctctggcct ggaacaactg aacttattga
    14521 actatgttag aaactacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat
    14581 gatatatcgg tgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga
    14641 tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga
    14701 taaaagattt cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga
    14761 agtcctgagt aaacacgttg acgttgaata ccgcttctct gccgagcctt atattggtgc
    14821 ctcatgcagt aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta
    14881 aggaattttt caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg
    14941 ttttggcttc attactccgg ctgatggcag caaagatgtg ttcgtacact tctccgctat
    15001 ccagggtaat ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga
    15061 cggccagaaa ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg
    15121 aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat
    15181 gctttatctt cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg
    15241 cggcctttag tatcctgccg gagttgtcat gtcgttttcc tgtccacttt gccatcagcc
    15301 tctttcgcgt gaaaaaaaca gctatatctg tccccaacga catcagtttg atatggcgaa
    15361 agaagggtat gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag
    15421 cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg
    15481 tgatgcaatt gtcgcccaac tgcgggaacg gcttgatgaa aaggccacgg cggtgctgga
    15541 tattggctgt ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac
    15601 cacgtttggc ctggatgtct cgaaggtagc gataaaagcg gcggcgaaac gctatccgca
    15661 ggtcactctt tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc
    15721 cataatacgt atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg
    15781 cggctgggtc attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat
    15841 ttacaatgaa gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca
    15901 gcagagtgcg gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt
    15961 gcagatgacg ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa
    16021 agaagtgttc gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc
    16081 gtggaagtgc gtccagagga tctggacgcc gatgccgatc agcaccagcc cgccgagaat
    16141 ttccgctttt ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgtcga
    16201 cataatcaag gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgaaggaa
    16261 agcaagacca acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag
    16321 tagccagaaa ccgtgccgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc
    16381 ctcaataatc attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtgattcca
    16441 ttcaaggaca aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt
    16501 ttcgacggca ccaaaaataa ggccggttcg caatgcttca gaaaa
//
LOCUS       DAJVPP010000087        15982 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVPP010000087 DAJVPP010000000
VERSION     DAJVPP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.087"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1991)
                     /gene="rnr"
                     /locus_tag="OQM34_002946"
     CDS             complement(<1..1991)
                     /gene="rnr"
                     /locus_tag="OQM34_002946"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1453305.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRL"
     gene            complement(2030..2455)
                     /gene="nsrR"
                     /locus_tag="OQM34_002947"
     CDS             complement(2030..2455)
                     /gene="nsrR"
                     /locus_tag="OQM34_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1453306.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(2660..3958)
                     /gene="purA"
                     /locus_tag="OQM34_002948"
     CDS             complement(2660..3958)
                     /gene="purA"
                     /locus_tag="OQM34_002948"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1453307.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4062..4259)
                     /locus_tag="OQM34_002949"
     CDS             complement(4062..4259)
                     /locus_tag="OQM34_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1453308.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4341..5345)
                     /gene="hflC"
                     /locus_tag="OQM34_002950"
     CDS             complement(4341..5345)
                     /gene="hflC"
                     /locus_tag="OQM34_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1453309.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5348..6607)
                     /gene="hflK"
                     /locus_tag="OQM34_002951"
     CDS             complement(5348..6607)
                     /gene="hflK"
                     /locus_tag="OQM34_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1453310.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(6693..7973)
                     /gene="hflX"
                     /locus_tag="OQM34_002952"
     CDS             complement(6693..7973)
                     /gene="hflX"
                     /locus_tag="OQM34_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1453311.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8049..8357)
                     /gene="hfq"
                     /locus_tag="OQM34_002953"
     CDS             complement(8049..8357)
                     /gene="hfq"
                     /locus_tag="OQM34_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1453312.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8443..9393)
                     /gene="miaA"
                     /locus_tag="OQM34_002954"
     CDS             complement(8443..9393)
                     /gene="miaA"
                     /locus_tag="OQM34_002954"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1453313.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9386..11233)
                     /gene="mutL"
                     /locus_tag="OQM34_002955"
     CDS             complement(9386..11233)
                     /gene="mutL"
                     /locus_tag="OQM34_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1453314.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11243..12580)
                     /gene="amiB"
                     /locus_tag="OQM34_002956"
     CDS             complement(11243..12580)
                     /gene="amiB"
                     /locus_tag="OQM34_002956"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1453315.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLCNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(12599..13060)
                     /gene="tsaE"
                     /locus_tag="OQM34_002957"
     CDS             complement(12599..13060)
                     /gene="tsaE"
                     /locus_tag="OQM34_002957"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1453316.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13032..14579)
                     /gene="nnr"
                     /locus_tag="OQM34_002958"
     CDS             complement(13032..14579)
                     /gene="nnr"
                     /locus_tag="OQM34_002958"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1453317.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            14578..15717
                     /gene="queG"
                     /locus_tag="OQM34_002959"
     CDS             14578..15717
                     /gene="queG"
                     /locus_tag="OQM34_002959"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1453318.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(15700..15753)
                     /locus_tag="OQM34_002960"
     CDS             complement(15700..15753)
                     /locus_tag="OQM34_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453319.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3660 a         4557 c         4023 g         3742 t
ORIGIN      
        1 tccagacgga cgaagaagcc aaagccagtg acgctggaaa ttacgccttt aaagacgtta
       61 cctacctggt cgagcatgaa gtcacacttc agccagtcag cgacatcgcg cgttgcttcg
      121 tcggcacgac gttccgccat cgaacagtgc tgacccagtt gcaacatctc ttccatcgaa
      181 taatggtagc cgccggtttc agtggtgttg ccctgatgcc cctgctcttt cgccagcaga
      241 tatttaatgg cgcggtgcag cgtcaggtca ggataacgac gaatcggcga agtaaagtgc
      301 gcataggact gcaatgccag accgaagtga ccacggtttt ctggatcgta aatcgcctgt
      361 ttcatcgagc gtagcagcat ggtttgcagc atttctgcat caggacggtc ggcaaccgac
      421 tccagcagtt ccgcgtagtc acgcggttcc ggcttgttac cacccggcag ctccagcccc
      481 agctccgcca gcactgaacg gaaagaggta atcgcttcgg tgctcggctt gtcgtgaata
      541 cggaacagtg ccggttcttt cgctttctca acgaaacgcg ccgccgagat attcgccaga
      601 atcatgcact cttcaattaa tttgtgcgcg tcgttacgct gggtctgttc gatacgttca
      661 atacggcgtt cagcgttgaa aatgaacttc gcttcttcgc tctcaaatga gatcccaccg
      721 cgttcttcac gggctttatc cagcacttta tagaggttat gcaactcttc gagatgctta
      781 accagcgggg cgtactgctc gcgcagatcc tgatcgccct gcagaatatg ccagactttg
      841 gtgtaggtca gacgcgcgtg agagctcatc accgcttcgt agaatttgta gcccgtcagg
      901 cggcctttcg acgaaaccgt catctcgcac accatacaca ggcggtctac ctgcgggttg
      961 agcgaacaca ggccgttaga gagcacttcc ggcagcatcg ggataacctg cgaagggaag
     1021 tacaccgacg tgccacggtt acgcgcttct ctgtccagcg gcgttggcgg acgcacatag
     1081 tagctgacgt cggcaatcgc gacccataaa cgccagccgc cgccgcgttt tttctcgcag
     1141 taaactgcat cgtcaaagtc acgggcgtct tcgccatcaa tggtgaccag cggtaaatcg
     1201 cgcaaatcga cacggcccgc ttttgcttct tccggcactt cttctttcag ccccgcaacc
     1261 tgttgctcaa cagcctgcgg ccagatatac ggaatttcat gggtacgcag agcgatatca
     1321 accgccatgc cggtgcccat attgtcgccc agcacttcga cgattttacc caccgctttg
     1381 gtgcggcgag tcggacgctg agtcagttcg actacgacca caaagcccat ccgcgcgccc
     1441 atgatctgat cgggcgggat taagatatcg aagctcagac ggctatcgtc aggaaccaca
     1501 aagccgacgc ccgcttcggt aaagtagcga ccaacaatct ggctggtttt tggcaccagt
     1561 acgcggacaa tacgcgcttc acgacgacct ttacggtcag cacccagcgg ctgcgccagc
     1621 acctgatcgc catgaatgca ggttttcatc tgctcgctgg agagatacaa atcatcttta
     1681 cgcccttcaa cccgcagaaa gccgtagcca tcacggtgac caataacggt acctttcacc
     1741 aggtcgaggc gttccggcag cgcatagcac tgacgacgag tgaagaccag ttgaccatcg
     1801 cgctccatcg cgcgcaggcg gcgacgcagg ccttcaagct gctcttcgcc ttcaatgtgc
     1861 agttctaccg ccagctcatc acggctggcc ggtttttcac gtttggttaa atgttcgagg
     1921 ataaattccc gactagggat gggattcgcg tatttttcag cttcgcgttc ctggaaagga
     1981 tcttgtgaca tctcggttcc tccgttgtca tctctgatga agattttcgt cactccacca
     2041 gcaataattt ataaagcggt tgattctctt caaccaaatc ggcaagcgtg tagttatcca
     2101 gttccgtaag aaaactttgc acggccttag aaagcgcctg tttcaaccga caggcaggtg
     2161 taatgtggca aaactcactg ctgcaattca ccagcgataa gggctccagc tcgcgcacca
     2221 catcaccaat acgtatcgca ctcgccggtt tacccaggcg aatgccgcca ttttttccac
     2281 gaacagcagt cacgtagccg gcacgactaa gttgattgat tattttgacc atatgattac
     2341 gggagacgcc gtagacgtca gtcacttcag aaatactggt catccgccct tctggcaatg
     2401 acgccatgta gatcagcgca cgtaatccgt aatcagtgaa actcgttaac tgcacatcaa
     2461 cctcaaaagg gaaatcggga aaaaatacat ttatattgat gataaaccag ccacaagctg
     2521 tgtcgctaat ttatttcagt tcggggaagg aaaaaagcga ggatttaaca ctgtgtcgga
     2581 tagcgtaatg gcttaaccga cacagcaatg tatcacactg ttagcccggc aggcaaaata
     2641 tctgccaggc gtaccagaat tacgcgtcga acgggtcgcg cagaatcatg gtttcagtac
     2701 gatccggacc ggtagagatg atatcgatcg gcacaccggt cagctcttca atacgcttga
     2761 tgtagttcag cgccgcctgc ggcaggccgc tacgatcttt cacgccgaaa gtggattcag
     2821 accagcccgg catggtttcg taaatcggct ctacaccttt ccagtcgtca gctgccagcg
     2881 gagtggtagt cacttcgcga ccatccggca tacggtaagc cacgcagagt ttaacctctt
     2941 tcaggccatc cagaacgtcc agtttagtca ggcagaagcc agacagggag ttcagctgta
     3001 ccgcacgacg aacggcaacg gtgtccagcc agccggtacg acgacgacga cccgtagttg
     3061 caccgaattc gttaccctgc ttgcagagga actcgccagt ttcatcaaac agttcggccg
     3121 ggaaaggacc tgcacctaca cgagttgagt aagctttgag gatacccaga acgtaatcaa
     3181 cataacgcgg gcccaggccg gaaccggtcg ccacgccacc agcagtggtg ttggaagaag
     3241 ttacgtacgg ataagtaccg tggtcgatat ccagcagcgt accctgcgca ccttcgaaca
     3301 tgacgaaatc gccacgctga cgcgcctggt cgagcaggtc agaaacgtca accaccatag
     3361 aagtcaggat gtcggcaaca gccatcgtat catccagaac tttctggtaa tcaaccgctt
     3421 cagctttgta gtagttaacc aactggaagt tgtgatattc catcacttct ttcagttttt
     3481 cagcgaaggt ttctttgtcg aaaaggtcgc caacacgcag accgcgacgt gccactttat
     3541 cttcataagc aggcccgata ccacgaccgg tggtgccgat cgctttcgcg ccacgcgctt
     3601 tctcacgcgc gttatccaga gcaacgtgat aatcaaggat cagcggacat gcttcagaca
     3661 gcagcagacg ctcacgaacg gggatgccac ggtcttccag ttctttcatc tctttcatca
     3721 gcgcggccgg agacagcaca acaccgttac cgatgatgct ggttacattc tcgcggagaa
     3781 tacctgatgg aataagatgg agaacggttt tttcaccgtt gattacgaga gtatggcctg
     3841 cgttgtgacc gccctggtag cgtacaacat atttagcccg ttcagtcaga agatcgacga
     3901 tcttaccttt accttcgtca ccccattggg tgcccagtac gacgacgttg ttacccattt
     3961 ttcaaaatca ccgtttgctt aaaaatggat tctaccatcg ctttttcaga gttacagcac
     4021 tttttgcact caaaaatgac ctcgatcagt ctgttttttg ttcagccaat cgttttcctc
     4081 aacatgtagt agaccacaac gcccgcaacc acaagtccac cgccaaaacg acgtaaaata
     4141 ttatcgggca aattggtcat cgcagagatc atcttcttcc atgctttcgg gtaaagcatc
     4201 ggccctaaac cttccagtac caaaaccagg gcaagcgcca gccagattgt cgaattcatt
     4261 cattatcctt atagaaaaag aaaaccaccg acatccctga ggatgcggtg gctttattga
     4321 cctgtaccgc agtcgttata ttaacgcgtt gcggaagtcg gcgtcttcat gtagcggaag
     4381 aaatcgctat ccgggctcat gaccatcacg tcctgattgc cagagaagct gttctcataa
     4441 gcacgcaggc tacggatgaa tgcgtagaag tccggatctt tactgaatgc atcagcaaac
     4501 agtttggctg cttcggcatc gccttcacca cgcataatgc ggccctgacg ctcagcttct
     4561 gccagcgttc tggtcacttc atagtcggca gtcgcgcgca gtttttccgc ttcttcctga
     4621 ccttgtgaac ggtgacgacg cgctaccgct tcacgctcgg cgcgcatacg gttgtagatc
     4681 gcttcagaca cttcggtcgg caggttgatc tgcttgatac gcacatcgac aacttcaata
     4741 cccagcgccg ccatactgtt cgggttgatg accggaactt tgcccttcgt ctcagccgtt
     4801 acgcgctctg ccgcttcggc aatggcgtta tctgctgccg gggtagtaac ttcatcttct
     4861 gtacccgcag aaccggagtt cagcgcgtca cgtacttcga gggtcagacg accacgggaa
     4921 tcggtgacga tatctttcac gtccaggcga ccaatttcag aacgcagacg gtcagagaac
     4981 ttacgtttca acagcacttc cgcttgcgaa atgtcgccac cacccgttgc caggtagtaa
     5041 cggctgaaat cgctgatgcg ccatttgatg taagagtcga cgatcaggtc tttcttctct
     5101 ttggtcacaa agcggtcggc ctggttgtcc atggtctgaa tacgtgcgtc gagcattttc
     5161 accgtttcaa tgaacggtat cttgaaatgc agaccaggct cataaaccaa aggtttgttg
     5221 tcatcgtcac gcagtacctt accaaaacgc agcgtaatac cgcgctcacc ttctttgacg
     5281 acaaagacag acatgtaaag cactaccagc acgatgatga taatcgcgat aactgactta
     5341 cgcatcgtta ttccccctga cgctggtagt cgttacgctg cgcgttggcg cggcgttggt
     5401 ccataatatc gccctgactg gtggacgacg tgttgcttgc tccactggtt gtggaggaag
     5461 aggctggcgg cagacgcagc agattgctgg caccgttatc gctcttcgcc gcaggggcgt
     5521 taccaccttt cagcatctgg tctaacggca gaaccatcag gttgccacct ttatcgttaa
     5581 ccagcacttt gcgggtgtta cccaacactt tttccatcgt ctcgatatac agacgctcgc
     5641 gagtaatttc cggcgcggct ttatattccg gcagaagttt agcaaagcgc gccacttcac
     5701 cctgagcttc caggatggtc tgggccttgt acgcacgcgc ctcttcgagg atacgttgcg
     5761 cctgaccgtt cgcacgcggc tgaacttcgt tggtatacgc ttctgcttca cgaatgtatt
     5821 gctgttcgtt ttcacgcgcg gcaatcgcat cgtcaaacgc cgcttttact tcttccggcg
     5881 gacgagcagc ctggaagttg acgtccagca gcgtgatacc catgtcatac ggacgaatcg
     5941 tctcttccag ttcgcgctga gtatcgctac gaatcacggt acgaccttcc gtcagaatgc
     6001 ggtccatggt gtatttaccg ataactccac gcagggcgct gtcggtagcc tgacgcaggc
     6061 tgtcatccgg gctggtcacg ctatacagat atttttccgg attggtgacg cggtactgca
     6121 cgttcatctc aacgcgcact acgttctcgt ccgacgtcag catcacacca gaagcggcca
     6181 gttcacgcac ggcttccacg ttcaccggtt tgacttcgtc gataaacgtc ggtttccagt
     6241 tcagacccgg ctcaaccaga tggctgaatt taccaaagcg tgttaccacg ccgcgttcgg
     6301 cttctttaat ggtatagaaa ccactggccg cccagataat gacaatcgct gccgctgcga
     6361 tggtaacgac acgaccgcca agctgcgggc gcgggccttg cgatgaactg ccaccgccag
     6421 atccggtgcc tttaccgccg cccagaccac cgagcttttt gctcagtttg cggaagatat
     6481 catctaaatc aggtggccct tgatcgcgac cgcctttgtt tccatttccc tcagagttgc
     6541 cgccaggttt gctgcttccc cacgggtcgc ggtcttgtcc gttattaccg ggctgattcc
     6601 acgccatgtt tgtgctccat atttgttatg cggtgatccc tgttgtcttt cgacgccaga
     6661 ggatatgact ccacgcttca gacgctacgc cgttagatca ggtaatcgat caacgccggt
     6721 tcttgtttac agaggcgacg ccagtcaacg atcggcatac gaacttgcag acttacgctg
     6781 ccgtcctcct ccatccactc tttttctatt gcctgaagct gataaaaacg actcctcaga
     6841 cgcccttcct gcggtggcag acgcaatgta tgctgcgcca cctcgccgga aagccgctcc
     6901 gtcaaagcct gaaaaagctg tggtatcccc gctccggtct gtgcggaaag ccagacacgg
     6961 atcggtttgt tctcttcgtc ccgatcaata cgcggttcga aatcttccag catatcgatc
     7021 ttgttcatca ccagcagggt tgggatctcg tgagcgtcga tctcttcaag aaccgtattc
     7081 accgcttcga tgttttcttg tacacgcaca tccgccgcat caatgacgtg cagcagtaat
     7141 gtggcttgcc gcgtctcttg taacgtggct ttaaatgccg ccaccagatc gtgcggcagg
     7201 tggcgaataa accctacggt atctgcaagt acggtttcac cgacatctgc aacgtcaata
     7261 cgccgcaacg tcgggtcgag ggtggcaaac aactggtctg ccgcgtagac ccgcgcttcg
     7321 gtgatgcgat tgaaaagggt agatttaccg gcgttggtat atcccaccag cgaaacagta
     7381 ggaacgtcgg ctttgatacg cgattgccgc ccctgctcac gctgcttttc aactctttcc
     7441 aggcgcgact gtatctgcac gatgcgatta cgcaacaaac gacggtcggt ttcgagctgg
     7501 gtttcacccg gaccacgcaa acctatcccg cctttctgtc tttcaaggtg ggtccagcca
     7561 cgcaccaggc gcgtagccag atggcgcagc tgcgccagct caacctgcaa cttaccctca
     7621 tgggtacgcg cacgttgggc gaaaatatct aaaataaggc cggtgcggtc gataacacga
     7681 cactcgcaca aacgctccag gttacgctct tgcgccgggc tcagggcatg gtcaaaaaga
     7741 acgaccgaag cacccatcgc tttgacagct tccgcaattt caactgcttt accttcacct
     7801 acaaaatact ttgggtgcgg cgctttacgg ctaccggtaa tcacctgcaa tgcttcgaca
     7861 ccggcggaag agaccagaga ttcaaactcc tggaggtctt ccatatcttt gtcttgcgta
     7921 aaatagatgt gtaccagtac cgcctgctca ccagcatcat aacggtcaaa caagcgtata
     7981 accctctaaa tagatcagcg gggaacgcag gatcgctggc tccccgtgta aaaaaacagc
     8041 ccgaaacctt attcggtttc ttcgctgtcc tgttgcgcgg aagtattctg cgcgctgcta
     8101 ccatgatggt agttactgct ggtaccgcca ccggcgttgt tactgtgatg agaaaccggg
     8161 cgagatggga caacagtaga aatcgcgtgc ttgtaaacca tctggctgac cgtgtttttc
     8221 aacaggatca cgaactgatc aaaagactcg atttgccctt gcagcttaat accattcacc
     8281 aaataaatag aaactggaac acgttcccga cgcagtgcgt tcaggaacgg atcttgtaaa
     8341 gattgcccct tagccattct ctcttttcct tatatgctta tttgtacttt gaacctttcg
     8401 attctgaaaa aattgcgcac gatacgtctc aattgtacac attcagcctg cgatagcacc
     8461 aacaacctgt aatacttcgt cacgcgcctg ttctggtttt tcactgtcaa gccagtgaac
     8521 cccttcccaa ccacgcagcc aggttatctg ccgcttcgcc aactgtctcg tggcgcaaac
     8581 acctcgataa accatttcat cgtatgagat ttcgccttca aggtaagacc acatctggcg
     8641 ataacccacg caacgaatgg aaggcaagtc cgtatgcaaa tctcctcggg caaaaagcgc
     8701 ccggacttct gcttcaaaac ctgaagccaa catctgatga aaacgctgct caatgcgttg
     8761 atggagcagt tcacggctcg ccggggcgat ggcgaactga tgcacctgat acggtagagc
     8821 gtctcctgac gtttgcgtca gttccgttaa agttttaccc gaaatgaaaa aaacttccag
     8881 tgcccgggaa agcctttgtg gatcatttgg atgaatcctt gctgccgcaa ccggatctac
     8941 ctcctgaagt tgacgatgca atgactccca accttgctct gccgcctgtt gctcaattct
     9001 ggcccgcact tccgggtctg ccgacggtag cggcgacaac ccttccagca atgccttgaa
     9061 atacaacatc gtaccgccca ctaacagtgg gatccgcccc gccgcggtga tatcggccat
     9121 ttccgccagc gcatcgcggc gaaaatcagc agccgagtaa gcctgcgacg gatcgcgaat
     9181 atccagcaat cggtgcggcg cggcgagtaa ctcttcagcg ttcggcttcg ccgtcccgat
     9241 aaccatccct ttgtaaataa gggcagaatc aacgcttatc aactctactg gtaaaatttt
     9301 acgcagctca atggctaacg ccgttttacc ggaggccgtc ggccccatca aaaaaatcgc
     9361 cttaggcagg ctcgccttac tgatatcact catctttcag ggcttttatc gccggatgta
     9421 aatcaacaga ttgtaacaga ccacccggcg gcgttttcac aagttgcgga cataaccgtt
     9481 ccacgtccgc cagcagggtt atggcctgtg ccattgacca ctgcgcatgt tcgctcatca
     9541 gatttcgtgc aatccactgc gcaatattgc caggttcgaa tacggactgc ttcgccaggt
     9601 agcctatcag ttcaggaatc aagatttgta aattttgttg gcgtaagggt aaaggcacgg
     9661 ccctgatggt cacatgctgt gcatctgact ggaaatcaat acccaattcc gctagggcag
     9721 actgcgcttt ttctaatgcc gatttttctt cgccagaaac ttttagccgc aacggaatca
     9781 gcagcggctg ggcgcaaacg ggcgcttcac ccggcgtcaa ttgtgcctga cgcagccaac
     9841 gttctgccac tggcaaggat aaaagtgaaa tgttgccatc gcgctccagc aacgcacagt
     9901 cggaatggac gatagtcagt acccgaccaa aactctgact gttcgccgca agtgcaggtt
     9961 cctgcggttc cggcgctttt ggtttttgca tcggcgcggg cgtttgcaaa agctggcgat
    10021 acacttcacc ttgctgtttc tggtagcctg gctgcgcatt cggccagggg gcagccggac
    10081 gactgcctga ggcgggcgca ggagagtagc gcggagctac cggctcacga gctgccggtt
    10141 ctgcaaagtg attgcgcccc gccgccacgc ggttttccgg gatggcacgc ggtgcgggtt
    10201 gtggttcatc gtccagcggt agcggcgttt ccagttgctg ttgtagcacg ctcagcacgc
    10261 cctgatagat aaaatcatgc accagacgcg actgatggaa acgcacttcg tgtttggcgg
    10321 ggtgtacgtt gacgtccacc tgatgcgggt cgatctccag atacaacaca aatgccggtt
    10381 gctgatcggc ccccagtttg tcttcgcagg cctggcggat cgcatgattg atcaggcgat
    10441 cgcgcatcat gcgaccattc acgtagcaat actgaatttc cgccagtgcg ggcgtagtgt
    10501 gatttggatc ggccacccag ccgcgcaggg tgagatcgcc gtgttgccat tcaatcgcca
    10561 gcgcttgttc gagaaaagcg gtgccgcaaa tcgcgcctaa gcgccgttct ttttgcccgc
    10621 cttccggcac tgcgcggtac tgacgcacaa ttttaccgtt atgcgacagg ttgatcgtga
    10681 cgtcgaaacg cgccagcgca atgcggcgga tgatttcatc aatatggcta aattcggttt
    10741 tctcggtgcg caggaatttg cgccgcgccg gggtgttgta gaacagatcc agcacctcca
    10801 gcgtcgttcc caccggatgc gctgccggtt taaccgtcac gtccatgtcg cgtccttcgg
    10861 cataggcctg ccaggcttcc tgctgttctg cggtgcgtga agtgagcgtc aggcgggaaa
    10921 ccgaactgat actcgccagc gcctcaccgc gaaagcccag gctgataatg gcttcgagat
    10981 cgtccagaga ggcgatttta ctggtggcat gacgagccag cgccagcgcc agctcatctt
    11041 ttttgatacc gcagccgtta tcacgaatgc ggataagttt cgccccaccg cgttcgatat
    11101 caatatcgat acgcgtcgca cctgcatcga ggctgttttc cactagttct ttgactaccg
    11161 acgcaggtcg ctcgaccacc tcacctgcgg caatctggtt cgccagttgt ggcggtaaga
    11221 cctgaattgg catcaatcgt ccttagtttg gcagcgtgcg atctggcgtc gtcaccgtac
    11281 tggcagtttg tgccgttgca ccctgcggcg cagattgcat cggatgcgca aggaagtaat
    11341 tgcacaggcc tttgtaaatg gcttctgcca gctgttgttg gtaatcgtcg ctcgccagca
    11401 aacgttcttc gctgttgttg ctgataaaac cggtttcgac cagtactgat gggatatccg
    11461 gcgagcgcag aacgccaagg ctggcgtgtt ctggtcgacg tttatgtatt tcgccaatgc
    11521 gttgcaactg actgatcata ctggtcgcta catcataccc tacccgctgg gaatgaccga
    11581 actgtaaatc cagcaccgcc tggcttaaat aggggtcaga ctgactgttc gccagcacat
    11641 cacccgcccc acccagcagc tccgacagtt tctcgtactg ctccagccag ctggccattt
    11701 cactgttggc gcgacggtta gagagcaccc atacggaagc gccagtcgca ctacggttcg
    11761 gtgcggcatc agcgtgaatc gacacgagga aattggcgtt ttgcttatgt gccacatcac
    11821 tgcgccccat caccgagata aagtaatccc cgtcacgggt taaaacgcct ttaaacatcg
    11881 gatcgtcatt gagcaaagta cgcaatttac gcgcgatggc gatggtgaca tttttctccc
    11941 gcgtaccacc ggggccgata gcgccagggt cctgaccgcc gtgtccggca tcaatagcga
    12001 tgataatttt atcgccagtg ttagccgtcg cgcgcgctgc cggacgcgtt accgtattac
    12061 tgctgataac acccgtagtg cggttacttt ccgttttaaa cggattgcgc gccggttcgc
    12121 tgacgcgcgg tgcgacaacc gcaggcgttt caacgcgttt cgcaaccacg ggcggcggag
    12181 gaggcggtgg cggcacatcg gcgttaatcg taaagacgac agtgtaattg ctgccattct
    12241 gccgcttcac cgcttcggtt ttaccgtttt cggtaagatc gaccaccagc cgtagcgttt
    12301 gtgcatcttt aggcgttcca gagcgaatcg ccttcaccag attattgccg ctgaacaaca
    12361 acggcagtcc ctgaatcacg cccgtttgtt tgatatcgag cgccacggtg cgtttgcttt
    12421 gatggctaaa cgcataatca ggatcgccaa taaaactcaa cgttatccgc gcctgttgat
    12481 taccgttaga aacctgaata tcagagagcg tcgcggcacc caccggcgtg cacagcagca
    12541 gcagcgtcgc taccaaccaa tttctgatgc gatacatcat cccgccacct ttcaaaggtt
    12601 aaccggctaa acgcgccagc aacaattcac ccgcagagga aaccgcactc acgcgcgcct
    12661 cacggccttg tgcctgataa tcaatgtgta tttcgacatc cgggtcagga agaacacctg
    12721 taccttgttg tggccactcc accaggcaga tggcatcgtt ggcaaaataa tcgcggatcc
    12781 ccataaactc cagctcctcg ggatcggcaa ggcggtacaa atcaaagtga tagaccatta
    12841 agttgtcgag cgtatagggt tcgaccagcg tataagtggg gcttttgaca ttaccctgat
    12901 gacccagagc ctgtaaaaag ccccggctaa aggtggtttt acctgcgcct aaatcgccat
    12961 acagatagat tacggttgcg ccatcgcagg ctttcgctac ccgctcgccc aggtctaatg
    13021 ttgcctgctc atcagggagc ggaattactc gattcatcat ggtttttatc agtcacttcc
    13081 gggttaacaa tacgctgtag cgtggaaaag agatcggttg ccagcatccc gcgcgttcca
    13141 aaacgcgccg ccagtacgtc agctgccgca ccgtgcgcga cacagcctgc acaggctgca
    13201 tcatacggcg acagtttttg cccaagcaat gcgccaataa taccagagag cacatcgccc
    13261 atgccgccgc tcgccatgcc tgcatttccg gcatcaataa tgcctaaagc gtcaggatgg
    13321 gcggcgacca cggttccggc acctttcagc accgctacgc cgccataacg ttgtaccaga
    13381 cgtttggcgc aatgtaagcg gtcactttca atttcagcga cggaacagcc taacaaccgt
    13441 gcggcctcgc taggatgcgg cgtaatcacg cgattgtgac gcttatcggg attgattgcc
    13501 agcaggttca atgcatcggc atcccacaac atcggtttgc gaaaattctc aactttttgc
    13561 agggcttttt tcccccactc ttgctggccc agaccgggac caatcaccac cacatcggcc
    13621 cattccaggc tttcggtaag agagtccata gtcagttcat gcaccatcaa ttctggtcgt
    13681 gcagtcagca gcggcgcaat gttctcactg cgggtcagta ctcggactaa accagcacca
    13741 gcacgtagcg ccgcttcccc cgtcatacga atagcccccg ccgtgccgtg atcgccaccg
    13801 ataatcacca gccgcccgtg atcgccttta tgcgaagtcg ggcgacgcgg tttcagccag
    13861 tgagaaagtt gttctgccga aaaccgctga attttcgtct cctgacctgc cagccagcta
    13921 tccagcccca gtgagtcaaa atgcagttgt cctgtaacat cccgcgcttt tccagtgagc
    13981 aagcctagtt tcagcgcaat aaaagtgatg gtgtgatcgg cgttgatcac tgcgcctggc
    14041 gtagcgccgg tttcagccag caggccggaa gggatatcaa ccgccacaat cggcgcagga
    14101 tgggaattag cgtggtcgat taactggcta atggattcgc ggggcgcttg ctgcaagccg
    14161 gtaccgagca gcgcatcaac aatcagatct accgattcgg gccagacaat attcgaagca
    14221 tggatctcgc cacccgcgtt taaccatgct tcgcgtgcca gcgcggcctc ttccggcaac
    14281 ggtttgtcgc tctcctgggc caacaacgtg acctcaatgc cgaccgcttt ggccagtcgc
    14341 gcgaccacgt agccatcgcc gccgttatta ccatgaccgc acagcaccag ccagtggcgg
    14401 gcgtcaggat acgccgaacg acacacctgg aatgcggcct cgccagcgcg aagcatcagc
    14461 tcatagagtg tgagccccag cacatctgcc gcctcgcgtt ctccgcggcg gatatcgtcg
    14521 gcgtaccaga cggtgtgtgg tatacttacg gggtttttct tcattgtatg gtccgtcatg
    14581 tcagagcccc tcgatctcaa tcagttagcg caaaaaatta aacagtgggg gctggaactg
    14641 ggctttcagc aggtaggtat taccgatacc gatctcagcg agtccgagcc caaactgcaa
    14701 gcatggctgg acaaacaata ccacggcgaa atggactgga tggcacgtca cggtatgctg
    14761 cgcgctcgcc cccatgagtt attacccggt acgctgcgtg taatcagcgt gcggatgaat
    14821 taccttcctg ctaacgccgc atttgccagc acgctgaaaa accccaaact cggctatgtt
    14881 agccgttatg cgctgggccg tgactatcac aaacttctgc gcaaccgact caaaaagctg
    14941 ggcgagatga ttcagcaaca ttgtgtttcg ctgaatttta gaccgtttgt cgattctgcg
    15001 cctattctcg agcgcccgtt agctgaaaaa gctgggctcg gctggacagg taagcactca
    15061 cttatcctca atcgcgaggc cggttcgttc ttctttttag gcgaattgct ggtcgatatt
    15121 ccgctgcccg tggatcaacc agtcgaggaa ggatgcggca aatgcgtggc ctgtatgacg
    15181 atttgcccga ccggtgccat cgtcgagcca tataccgtcg atgctcgccg ctgtatctct
    15241 tatctcacca tcgaacttga aggggcgctc ccggaagagt tgcgaccgtt aatgggaaac
    15301 cgtatttacg gttgcgatga ctgccagctt atctgcccgt ggaatcgcta ttcgcaactc
    15361 actacagaag acgatttcag cccgcgtaag ccgctacacg caccggaact cattgagtta
    15421 tttgcctgga gcgaagagaa gtttttaaaa gtcacggaag gatcggcgat tcgccgtata
    15481 ggtcacctgc gctggctgcg taatatcgcc gtggcattag gcaatgcccc ttgggatgaa
    15541 acgattttga cagcgctgga aagtcgtaaa ggtgagcacc cacttctcga tgagcacata
    15601 gcgtgggcga ttgcgcagca aatagagaga cgaaatgcgt gcatagtcga agtgcaattg
    15661 ccgaaaaaac agcgtctggt tcgggtgatt gaaaaagggt taccgcgtga cgcctgattc
    15721 atccacagct tgtgaataaa aataaaaacg cattgcaatt caagacgaat aaaatcgtca
    15781 agtgatcgac tcgacaaaac ggaatcttga tttttaactt atttatcaat aggttaaaaa
    15841 ataccattca ccgtgcgaag tttctttgaa attaagatta cagcctgagg ctgtggataa
    15901 ctctgtttac aagagtttgt cagagacaac aaaaaccagc aataacgcat cgcgtcgctg
    15961 tagatatttt attgagagaa ga
//
LOCUS       DAJVPP010000088        15925 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVPP010000088 DAJVPP010000000
VERSION     DAJVPP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.088"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..237
                     /locus_tag="OQM34_002961"
     CDS             <1..237
                     /locus_tag="OQM34_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCS1453320.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="OQM34_002962"
     CDS             247..885
                     /locus_tag="OQM34_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS1453321.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="OQM34_002963"
     CDS             888..2051
                     /locus_tag="OQM34_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1453322.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..3760
                     /locus_tag="OQM34_002964"
     CDS             2135..3760
                     /locus_tag="OQM34_002964"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1453323.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="OQM34_002965"
     CDS             complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="OQM34_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1453324.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="OQM34_002966"
     CDS             complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="OQM34_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1453325.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4812..5561
                     /gene="yjfP"
                     /locus_tag="OQM34_002967"
     CDS             4812..5561
                     /gene="yjfP"
                     /locus_tag="OQM34_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1453326.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="OQM34_002968"
     CDS             complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="OQM34_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1453327.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="OQM34_002969"
     CDS             complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="OQM34_002969"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1453328.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7840..9237
                     /gene="ulaA"
                     /locus_tag="OQM34_002970"
     CDS             7840..9237
                     /gene="ulaA"
                     /locus_tag="OQM34_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCS1453329.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9253..9558
                     /gene="ulaB"
                     /locus_tag="OQM34_002971"
     CDS             9253..9558
                     /gene="ulaB"
                     /locus_tag="OQM34_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1453330.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9568..10032
                     /gene="ulaC"
                     /locus_tag="OQM34_002972"
     CDS             9568..10032
                     /gene="ulaC"
                     /locus_tag="OQM34_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS1453331.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10046..10696
                     /gene="ulaD"
                     /locus_tag="OQM34_002973"
     CDS             10046..10696
                     /gene="ulaD"
                     /locus_tag="OQM34_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1453332.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10706..11560
                     /gene="ulaE"
                     /locus_tag="OQM34_002974"
     CDS             10706..11560
                     /gene="ulaE"
                     /locus_tag="OQM34_002974"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1453333.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            11560..12246
                     /gene="ulaF"
                     /locus_tag="OQM34_002975"
     CDS             11560..12246
                     /gene="ulaF"
                     /locus_tag="OQM34_002975"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1453334.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12375..12650)
                     /locus_tag="OQM34_002976"
     CDS             complement(12375..12650)
                     /locus_tag="OQM34_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1453335.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12977..13372
                     /gene="rpsF"
                     /locus_tag="OQM34_002977"
     CDS             12977..13372
                     /gene="rpsF"
                     /locus_tag="OQM34_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1453336.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13379..13693
                     /gene="priB"
                     /locus_tag="OQM34_002978"
     CDS             13379..13693
                     /gene="priB"
                     /locus_tag="OQM34_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1453337.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13698..13925
                     /gene="rpsR"
                     /locus_tag="OQM34_002979"
     CDS             13698..13925
                     /gene="rpsR"
                     /locus_tag="OQM34_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1453338.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13967..14416
                     /gene="rplI"
                     /locus_tag="OQM34_002980"
     CDS             13967..14416
                     /gene="rplI"
                     /locus_tag="OQM34_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1453339.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14487..15050)
                     /locus_tag="OQM34_002981"
                     /pseudo
     CDS             complement(14487..15050)
                     /locus_tag="OQM34_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            15114..15925
                     /locus_tag="OQM34_002982"
                     /pseudo
     CDS             15114..15925
                     /locus_tag="OQM34_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3738 a         3887 c         4294 g         4006 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acattttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaaccg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccaataa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gttccggcaa
     3421 tattatgtgc ctggatgtgt tgcgcgttct caataagcaa gcgggcgtat acgacttatt
     3481 gtcggaagca tttgtggaag tgaaagggca ggatcgctat tttgatcgcg cggttcgtcg
     3541 tttacagcag cagctgcgta agccagctga agaactgggg cgagagatta ctcatcagct
     3601 attcctgctg ggctgcggtg cgcaaatgtt gaaatatgct tctccgccaa tggcgcaggc
     3661 gtggtgtcag gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc agatccagaa
     3721 tgatttattg ctgcgggcga cggggggagt gtgtgtgtaa gcgtatacga ctgatgcgac
     3781 gctggtttcg attaactaaa tgaaatatgt gaaaattgta ggccggacaa ggcgctcgcg
     3841 ccgcatccgg cattgttcat ttgctgtaga tgacactcat gcatacagta tcgcctgtac
     3901 gcgccagtta tcaggctgct ggtcttcgta catggtaata atgcggtacc atgacgcacc
     3961 ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc tgcggatccc ctgaaatatt
     4021 gctaactgag atctgaccta tttcattcag gcccgtcacg cagtcagcac tggcgaattc
     4081 tgcagactgt gccgtggcgc tcgcaggata agtcactaat tgcagcgagg gtgaggcaag
     4141 taattgtttc atcgtcagct ccatttttcg tttccccaca tgttgcaggg cattccatcg
     4201 cggtaataaa cgcccaagct tccagccggt aatttattgt gcacaggtaa acgcataggg
     4261 acgcaaagca atgtaaagct gactatgtaa gttgtctgat ttatttacgg tacaaaatgg
     4321 cttgtgaata ccactgtcct gtcacgatgg tttcgtccac catgacgaca acgtaataat
     4381 cagcttttgc agcgacagct tttgctctga tttctgctaa tgcgtcatcc ggagaacccc
     4441 gaaccatcgt gcttacgcta cctattcgct gtaacccttg cgtctggtcg cggcgaatct
     4501 cttgcggatg gtccgttact ggcggtgctg gctgtggcgt accttgcagt gcgctacagg
     4561 cacttaacat caacaccaat aataaactgg caaaccggta aataacgcta ttacgtttcc
     4621 tgctaaccat agtgtagtgc cttattaatt taactttggg ggaatgttcc cgaattgtta
     4681 cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt aacccacatc tcaacattat
     4741 gaactaatac cgccagcaga aacttgtgct aaggatttct tcatgtcata ccagacaaaa
     4801 aggagagaca gatgattgaa atagaatcac gcgagctggc agatattccc gttcttcatg
     4861 cttatcctgt cgggcaaaaa gataccccgt taccgtgcgt aattttttat cacggcttta
     4921 cttcatccag cctggtgtat agctattttg ccgttgcgct ggcgcaggct ggtttgcggg
     4981 tgatcatgcc ggatgcgccc gatcacggta gccgttttag tggcgacgaa gcgcggcggt
     5041 taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga attcactact ttacgtgcgg
     5101 caatagccga agaaaactgg ctgcttgatg accgtctggc agtcggtggc gcgtcgatgg
     5161 gcgcgatgac ggcactggga attaccgctc gccaccccac ggtgaaatgt accgccagca
     5221 tgatgggatc gggctatttt acatcgctcg cccgttcact gtttccaccg ctgatacctg
     5281 aaacggcagc acagcagaat gaattcaata aaattgtcgc gccgctggca gagtgggaag
     5341 cgacaaacca cctggaacaa cttggggaca gacctctact gctgtggcat ggcctcgacg
     5401 atgacgttgt gcctgccgac gaatcactac gtttgcagca ggccttaagc gagacgggac
     5461 gggataaact gctaacctgt tcatggcagc caggcgtgcg tcaccgtatt acgcctgagg
     5521 cgttagatgc tgccgtgaca tttttccgcc agcatcttta aacacgcaga atgctgaccc
     5581 cttgcgcttc cagttgttgc aggatttccg ggttagcatt tttgccggtg atcagcatat
     5641 cgatttgatc ggcacggcta aaaagcattc ccgcgcgttc gccaatctta ctgctatcaa
     5701 ccagtaccac cagtttccct accacgctca gcatcttctg ctctgccatt gctgtcagca
     5761 tatcggtttt atacaaccct tctgcggtca gcccttttcc gctggtaaac atccagtgcc
     5821 cggcatagag actgttttcg ctgccctgcg ggcttaaagt gatggactga cttttgttgt
     5881 actgtccacc cataatgatc acgctgtcat gttcttgatc gatcaggtaa tttgccagcg
     5941 gtagataatt agtgatgatt tgcactggct tgccacacat ttcccgccca agcagaaacg
     6001 cggtggagcc gcagttgatg actacgcttt cgcccggatt aaccagctgc gaggccgctt
     6061 tagcaatacg tactttttca tcgtgattct gcgcctgatg cagattcatc ggcgtccagc
     6121 gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac ctttttcagt ttgccgcttt
     6181 cgccaagttt attgatatcg cgtcgcgcag tggcaggcga aattcccaga cgctcaacga
     6241 ctttctcaac ggtcacaaag cccaattgtg cgagcatttc caggaggatt tgatgtcttt
     6301 gtgcttcagt catgagctat tccgataaaa tttgattttt ttagatgata tttgaaatag
     6361 ccaggaaata ctacgccgga tagcgcgaac ttctccacgc tatccggtat aaacgacaga
     6421 ttacaggaat gacttgaacg gcagatccgg ttcaatagtg aagcaatcat cgaaaccgcg
     6481 cggatagtgg tactcgaagt tgtctttatc cagcggccag gtaaatttgc cgccaacctg
     6541 ccagataaac ggcttgaagc catacttcag acgatctttt ttgatctccc acagcacgcg
     6601 gatctcttgc ggatcggcct ggaagtttga ccagatgtcg tggtggaacg ggataaccac
     6661 tttcgcattc agggcttcac ccatacgcag catatcggcg ctggtcattt tgtcggtaat
     6721 accgcgcggg ttctcaccat acgatcctaa cgccacatcg atctgatgtt cgttaccgtg
     6781 cttcgcataa tagttagagt agtgggaatc gccactgtga tatagggaac cgccaggagt
     6841 tttgaacagg tagtttactg cgcgatcgtc catgccatct ggcagtacgc cagccgcttt
     6901 ttgatcggca ggcagggtga tcagtgcagt acggtcgaaa gcgtcaagcg catgaatttc
     6961 aatgtctttc acttttacaa catcgcccgg tttgaccacg atgcaacgct ctttcggtac
     7021 gccccagcca atccacaaat caacgcaggt tttcggtcca atgaatggta cgtcatcagc
     7081 acagttctgc atcacggcag cagcgacgtt aacgtcgata tgatcgttgt gatcgtgagt
     7141 cgccagtacc gcgtcgatct ggcgaatcgc aaacggatca agaacaaacg gggtggtgcg
     7201 taggtttggc tgcagttttt tcacgccagc catgcgctgc atctggtgac cctgtttcat
     7261 taacgggtta ccgtgacttt gtttgccagt gccgcaccag aaatcaacgc aaacgttggt
     7321 gccaccttct gatttcaacc agatcccggt gcagcctagc caccacatcg caaatgtgcc
     7381 aggggcgacc tgctcttgtt caatttcttc attcagccag ctaccccact ccgggaaagt
     7441 gctcaggatc caggattctc gggtgatact tttcacttta ctcatcgcca tttaccttca
     7501 tgatagttca attcgaatca acatgtgatt ggttttgatt aatcctgaca ctattccttc
     7561 aggaaggcaa tgaccatttt ttgacttttt gccagggaag ttgttgttga ttttgagtgt
     7621 ggaaagattt aatggaatgt gtagttaaat taactaaatg aatttaaata aataattgtt
     7681 tcgttgtgtg aatcacactc tatccgtgtg gaattatttg cgggtcgcgt cacatttaat
     7741 cataaataat cttgttgtga ttacttttga aaattagagt gagtgcacaa cattccgggt
     7801 gtgcggaata cccggttacc tcttcttcag gagatcgtta tggagatcct ctacaacatc
     7861 tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg tattgtgacc
     7921 tgtctgggct acatcctact gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa
     7981 accataattg gtttcatgtt gttgcaggca gggtccggca tcctcaccag caccttcaaa
     8041 ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc cgatacttac
     8101 gcgtcgatga tggcaaccat cgaccgcatg ggcgatgcct atagctgggt aggttacgcg
     8161 gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt
     8221 cgtaccatca tgctgactgg tcatatcatg ttccagcagg cggggctgat tgccgttacg
     8281 ttgtttattt tcggctactc catgtggaca accattatct gcacggcgat tctggtttcg
     8341 ctctactggg gcattacctc caacatgatg tacaagccga ctcaggaagt gacggacggc
     8401 tgcggtttct ccatcggtca ccagcaacag tttgcatcat taattgccta taaagttgcg
     8461 ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg ctggctgaat
     8521 attttccacg acaacatcgt ctccacggcg attgtcatga ccatcttctt tggtgccatt
     8581 ctgctctcct tcggtatcga caccgtgcag gcgatggcag gcaaagtgca ctggacggtc
     8641 tatatcctgc aaaccggttt ttccttcgcg gtggcgatct tcatcatcac tcagggtgtg
     8701 cgcatgtttg tggcggaact ctctgaagcg tttaacggta tctcccagcg cctgatccct
     8761 ggtgcggttc tggcgattga ctgtgcggct atttatagct tcgctccgaa cgccgtggtc
     8821 tggggcttta tgtggggcac catcggtcag ctgattgcgg ttggcatcct ggtcgcctgc
     8881 ggttcctcga tcctgattat cccaggcttt atcccgatgt tcttctccaa cgccaccatc
     8941 ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc tgaagatttg tctggtgatg
     9001 gggatgatcg aaatctttgg ttgcgtctgg gtggtgaaac ttaccggtat gagtgcctgg
     9061 atggggatgg cggactggtc gattctggca ccgccgatga tgcagggctt cttctccatc
     9121 ggtatcgcct ttatggccgt catcattgta attgcactgg cttatatgtt cttcgctggc
     9181 cgcgcactgc gcgcagaaga agatgcagaa aaacaattgg cagaacagtc tgcttaataa
     9241 ggagttttga ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca aggcagttcc
     9301 atgatcatga agatgaaagt ggaccagttt ttaacccaat caaacattga ccacacggta
     9361 aatagctgcg cggttggcga atacaaaagc gagttgagcg gcgcggatat catcatcgct
     9421 tctacgcaca ttgcgggcga aatcaccgtg accggcaaca aatacgtggt tggcgtgcgc
     9481 aacatgctct ctcctgccga ctttggcccg aaactgctgg aagtgatcaa agcgcatttc
     9541 ccgcaggatg tgaagtaagg acgcgccatg aaattacatg attcgctggc ggagaataaa
     9601 tccatccgcc tgcaggctga agctgagaca tggcaggacg cagtaaaaat cggcgttgac
     9661 ctgctggtgg cggcggatgt tgtcgagccg cgttactacc aggcgattct ggatgcggtt
     9721 gaacagcacg gtccgtactt tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg
     9781 gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc
     9841 aaccacgaag ataacgaccc ggtggatatc ctcatcacca tggcggcggt cgatgccaac
     9901 acgcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga agaaaatttc
     9961 gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac
    10021 gcggcagctt aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa
    10081 ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat
    10141 cgaagtgggt accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc
    10201 gctctacccg cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg gcaaaattct
    10261 ttctcgtatg tgcttcgaag ccaacgctga ctgggtgacc gtgatttgtt gtgcggatat
    10321 caacaccgcc aaaggtgcgc tggacgtagc aaaagagttt aacggcgacg tgcagatcga
    10381 actgaccggt tactggacct gggaacaggc gcaacagtgg cgcgacgcag gtattcagca
    10441 ggtggtttat caccgcagcc gtgacgcgca ggccgctggc gtggcgtggg gtgaagccga
    10501 tatcactgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggtggcct
    10561 ggcgctggaa gatctgccgc tgttcaaagg cattccaatt catgtgttta tcgccggtcg
    10621 cagcatccgt gatgccgctt ctccggttga agccgcgcgt cagttcaaac gttccatcgc
    10681 cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga
    10741 aaaagcgctc cccgccgggg agtgctggct ggaacgcctg cgactggcaa aaacgttggg
    10801 cttcgatttt gtcgagatgt cggtagatga aactaacgag cgcctgtcgc gcctcgactg
    10861 gagccgcgaa cagcgtctgg cgctggtgaa tgctattgtt gaaaccggcg tgcgtgtgcc
    10921 gtccatgtgc ctttctgccc atcgtcgttt cccgttgggc agcgaagatg acgcggtgcg
    10981 ggcgcagggg ctggagatta tgcgtaaagc catccagttc gcccaggacg ttggcattcg
    11041 cgtgatccag ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg
    11101 tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcgc aggtgacgct
    11161 ggcgatggag atcatggatt atccgttgat gaactccatc agcaaggcgc tgggttacgc
    11221 gcactatctc aacaacccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg
    11281 ggacaacgat gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtgcatgt
    11341 gaaagacacc aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg tggtggattt
    11401 cgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat
    11461 gtggagcgaa acggcggaag acccggcggc agaagtggtg aaagcgcgtg attgggtgaa
    11521 agcgcgcatg gccaaagcag gcatggtgga ggcggcataa tgcaaaagct aaaacagcag
    11581 gtgtttgaag ccaacatgga tctgccgcgc tatgggctgg tgacctttac ttggggcaac
    11641 gtcagcgcta tcgaccgcga acgcgggctg gtggtgatca agcccagcgg cgttgcctac
    11701 gaaaccatga aagcagacga tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg
    11761 gagtatcgcc catcttccga cactgcgacg catctcgaac tctaccgtcg ttacccgtcg
    11821 cttggcggca ttgtccatac ccactccact catgccaccg catgggcgca ggcggggctg
    11881 gcgatcccgg cattaggcac cacgcacgct gactacttct ttggtgatat cccgtgtacg
    11941 cgtggattaa gcgaagaaga ggtgcagggt gagtatgaac tgaacactgg caaagtgatt
    12001 atcgaaacgc tgggcaacgc cgagccgctg catacgccgg gaattgtggt gtatcagcac
    12061 ggaccgttcg cctgggggaa agatgcccac gacgcggtgc ataacgcggt agtgatggaa
    12121 gaagtggcga aaatggcgtg gattgcgcgc agtattaatc cacaactcaa tcatatcgac
    12181 agctacctga tgaataaaca cttcatgcgt aagcacggtc ctaacgctta ttacgggcag
    12241 aagtaaaaca cgcgctgcgg aaatttcctt cctcgggaga taactggtct aattccgcag
    12301 ccgtttttca aaaaaagccc cctgcgaagg gggcaaagca aactatggca atgtttcgtt
    12361 ggttatacct ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc gttactcccc
    12421 ggttcccgca tcagaattac gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt
    12481 aatgcctgac gtgcttcgct ttcagtggcg aaattatgat tgatataaat cacgcctaag
    12541 ctttgcacat cgtccatatt tctggcctgg tggttattaa tttcaatggc tgcccatgta
    12601 tttgcactta gcaaaagcac agccagaagg gctaaaactc gactgaacat agatacctcc
    12661 tcgacggctg actttgtgtg ctctcctctg tgatgatctt ctgatttaat tttaatcaat
    12721 gataaagaag ttgatggtga ccatttctga tgcagttgtt caaaaaaaca ctgtgacgga
    12781 atgtgatgaa cttcaaatca gcggtttaga ggttatttgc gaaaggggag atttatttct
    12841 gctccgccct tgagtttagc ggggtatgcc agtactataa cgcgtcattt ttcagccgac
    12901 ctttaacacg ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc
    12961 cgtaaggagc aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag
    13021 cgaacaggtt ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa
    13081 gatccaccgt ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca
    13141 caaagcacac tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga
    13201 aactaccttc cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc
    13261 tgttaccgaa gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga
    13321 tttcgcaaac gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat
    13381 gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc
    13441 atcaggaatt cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg
    13501 ctttcaccgg caggcatggt gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc
    13561 cattactcac agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca
    13621 caaggcaaag aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga
    13681 ttctggagac tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg
    13741 cggaaggcgt tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg
    13801 aaagcggtaa gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc
    13861 tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc
    13921 agtaatcggt cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct
    13981 gcttgataaa gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta
    14041 tgctcgtaac ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga
    14101 attcttcgaa gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagctgc
    14161 taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg
    14221 cgacgaaggt aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc
    14281 agctggcgtt gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac
    14341 tggcgaacac gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa
    14401 cgtagtagct gaataattcg ttattcaacg agacgtaaaa acgccgacca ttggtcggcg
    14461 ttttgctttc tatttttcgt caggcattaa tttcgcaagt agatccattc accattctct
    14521 tgtgtccata agtcaggatg gtttgctggc gttagatgca tacgatcact ccttatgtct
    14581 cggtcctgcg gcactcgctc gaaacctaaa cccgagtaga ttgcggctga atttgataag
    14641 gaacgtagac caattttcaa atcatggggt aaatcagaat acgcgtttag caatgcagca
    14701 atgatgaatc cgagggcatt agaaacgcct gtaacagtgt gaagttggtc gatataaaga
    14761 tcgattgttg gtagttcaga tgaccagcaa ttaggcattt gggcaggaag ggttacaacg
    14821 tatcctatcg tttggccggt atagtctgga gtactataac caggtcccaa aacaacctcg
    14881 tgtattgtgt atttactgtt gggttcgaag gattcgacat cttgcaataa atatttcagt
    14941 tctgcggtta acctcgcacc cgcgtaagct ttattgtatt tctgggcgtg aatattatct
    15001 aatgtagctt catggttcca tccaaaagaa ttatcaccat tcattgttac tgaatcgcca
    15061 cgggtttaac agacacctca gagtcattta agatggctta aagagaggtg cccatgagcg
    15121 gtaagcgtta tcccgaagag tttaaaactg aagcagtcaa acaggttgtt gatcgcggtt
    15181 attctgttgc cagcgttgca acacgtctcg atatcaccac ccacagcctt tatgcctgga
    15241 taaagaagta cggtccggat tcttccacta ataaagaaca gtcagatgct caggccgaga
    15301 tccgccgtct ccagaaagag ctgaaacggg ttaccgacga acgggacata ttaaaaaaag
    15361 ccgcggcgta cttcgcaaag ctgtccgact gaggtacgcc tttatccgtg acaactcctg
    15421 ttgctggcct gttcgcctgc tctgtcgggt gctggatgtt catcccagtg gtttttacgc
    15481 ctggcttcag cagccgcatt cacaacgcca tcaggcagac ctgagactga caggacagat
    15541 taaacagttc tggctggaat cgggatgcgt ctatggttat cgcaaaatcc atctggatct
    15601 gcgtgacagc gggcaacagt gcggagtaaa cagagtctgg agactgatga aacgtgtcgg
    15661 aataaaggct caggtcggat accgaagccc gcgggcacgt aaaggcgagg ccagtatcgt
    15721 gtcacccaac aggctccagc gacagttcaa tccggatgct cctgatgagc gttgggtaac
    15781 ggacataacc tacatcagga cccacgaagg ctggctgtat cttgccgttg ttgttgatct
    15841 gttctcacgc aaaattatcg gctggtccat gcaatcccgg atgacaaagg acattgtcct
    15901 gaacgcactg ctgatggctg tatgg
//
LOCUS       DAJVPP010000089        15477 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVPP010000089 DAJVPP010000000
VERSION     DAJVPP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.089"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1050
                     /gene="citC"
                     /locus_tag="OQM34_002983"
     CDS             <1..1050
                     /gene="citC"
                     /locus_tag="OQM34_002983"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1453340.1"
                     /translation="NDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTRDEK
                     LIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYEALF
                     RQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNGHRY
                     LIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISRATF
                     PCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMRYWL
                     ETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQNLL
                     EHSRQDAAARQKTPA"
     gene            1065..1361
                     /gene="citD"
                     /locus_tag="OQM34_002984"
     CDS             1065..1361
                     /gene="citD"
                     /locus_tag="OQM34_002984"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1453341.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1358..2266
                     /gene="citE"
                     /locus_tag="OQM34_002985"
     CDS             1358..2266
                     /gene="citE"
                     /locus_tag="OQM34_002985"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1453342.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2277..3809
                     /gene="citF"
                     /locus_tag="OQM34_002986"
     CDS             2277..3809
                     /gene="citF"
                     /locus_tag="OQM34_002986"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS1453343.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3813..4364
                     /gene="citX"
                     /locus_tag="OQM34_002987"
     CDS             3813..4364
                     /gene="citX"
                     /locus_tag="OQM34_002987"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCS1453344.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4339..5217
                     /gene="citG"
                     /locus_tag="OQM34_002988"
     CDS             4339..5217
                     /gene="citG"
                     /locus_tag="OQM34_002988"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCS1453345.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5268..6730
                     /gene="citT"
                     /locus_tag="OQM34_002989"
                     /pseudo
     CDS             5268..6730
                     /gene="citT"
                     /locus_tag="OQM34_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            6844..7650
                     /gene="rna"
                     /locus_tag="OQM34_002990"
     CDS             6844..7650
                     /gene="rna"
                     /locus_tag="OQM34_002990"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1453346.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPPPGNCGKTFVIDKAGY"
     gene            7879..8289
                     /gene="rnk"
                     /locus_tag="OQM34_002991"
     CDS             7879..8289
                     /gene="rnk"
                     /locus_tag="OQM34_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1453347.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8520..9758)
                     /locus_tag="OQM34_002992"
     CDS             complement(8520..9758)
                     /locus_tag="OQM34_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCS1453348.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGVTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            9979..10407
                     /gene="uspG"
                     /locus_tag="OQM34_002993"
     CDS             9979..10407
                     /gene="uspG"
                     /locus_tag="OQM34_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1453349.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10528..12093)
                     /gene="ahpF"
                     /locus_tag="OQM34_002994"
     CDS             complement(10528..12093)
                     /gene="ahpF"
                     /locus_tag="OQM34_002994"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1453350.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12338..12901)
                     /gene="ahpC"
                     /locus_tag="OQM34_002995"
     CDS             complement(12338..12901)
                     /gene="ahpC"
                     /locus_tag="OQM34_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1453351.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13273..14019
                     /gene="dsbG"
                     /locus_tag="OQM34_002996"
     CDS             13273..14019
                     /gene="dsbG"
                     /locus_tag="OQM34_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1453352.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14229..15131
                     /locus_tag="OQM34_002997"
     CDS             14229..15131
                     /locus_tag="OQM34_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1453353.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEYPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15278..15477
                     /locus_tag="OQM34_002998"
                     /pseudo
     CDS             15278..15477
                     /locus_tag="OQM34_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3785 a         4035 c         3787 g         3870 t
ORIGIN      
        1 aatgatattt tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa
       61 ttcctgcatg aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc
      121 cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgct
      181 atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac
      241 ctcgcctatg agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg
      301 ctgttccgcc agtgcggttt ttccacgctg acctgcgtac ccggcgtgat ggtgctgatg
      361 gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca
      421 gggaacaaga ttggctgcat tgtgatgaac gccaatccct ttacgaatgg tcaccgttat
      481 ctgattcaac aagctgcggc acagtgcgac tggttgcatc tgtttttagt caaagaagat
      541 tcttcacgct tcccctatga agaccggctg gatctggtgt taaaaggcac cgccgatatt
      601 ccacgcctga ctgtgcatcg cggctcagaa tacatcatct cccgcgctac gttcccttgc
      661 tacttcatta aagaacagag cgtcattaac cattgttaca ccgaaattga tctgaagatt
      721 ttccgtcagt acctcgctcc cgcgcttggt gtaactcacc gctttgtcgg tactgaaccc
      781 ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact
      841 atctccgcag cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg
      901 atatccgctt cccgggtacg tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg
      961 ctggtccctg cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac
     1021 gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc
     1081 ccgccgttgc aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata
     1141 cgcaggatat cgacctgcaa atcaatagca gcgttgagaa acagtttggc gatgcaattc
     1201 gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg
     1261 atgacaaagg cgcactggac tgcattttac atgcacgact ggaagccctg ctggcacgcg
     1321 ccagcggtat cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca
     1381 acgtaaaact cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt
     1441 cagcaactcc ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgtggc
     1501 attacgtgaa aaagacaccg cccgccgcat ggtttatcac gcgctgcaac atccgctgta
     1561 tcgcgatatt gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg gtgttaacga
     1621 cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc
     1681 tcaggatgtt ctggatattg aaaaagagat cctgcgtata gaaaaagcct gtggtcgtga
     1741 acccggcagc accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt
     1801 ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt
     1861 gcgcaacctg cgtacagaac gctccccgga aggaactgaa ctgctgttcg cacgctgttc
     1921 cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc
     1981 taacaacgaa gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg
     2041 caaatcgctg atcaacccgc gtcagattga tctgctgcac aacctctacg caccgaccca
     2101 gaaagaagtg gatcacgccc accgcgtcgt agaagccgct gaagccgccg ctcgcgaagg
     2161 cctcggcgtg gtttccctga acggcaagat ggtggacggt ccggttatcg atcgcgcccg
     2221 tctggtgctc tcccgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga
     2281 cgcagaaaat tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcactg
     2341 aatgcgatct tgccgctttc cagaactcac caaagcaaac ctaccaggct gaaaaagcgc
     2401 gcgatcgcaa actatgcgcc aacctggaag aagcgattcg tcgctcaggt ttacaggacg
     2461 gcatgacggt ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga
     2521 tgaacgtcat cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc tccctgagtg
     2581 attgccatgc gccgctggta gaacacattc gccagggcgt ggttacccgc atttatacct
     2641 ctggcctacg cggtccactg gcggaagaga tctcccgtgg tctgctggcg gaaccggtgc
     2701 agatccactc tcacggcggt cgtgtgcatc tggtacagag cggcgaactg aatatcgacg
     2761 tggctttcct cggcgtcccg tcctgtgatg aattcggtaa tgccaacggc tacaccggta
     2821 aagcctgctg cggctccctc ggctatgcaa tggttgatgc cgacaacgca aaacaggtcg
     2881 tgatgcttac cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc
     2941 aggttgattt gatcgtcaaa gttaatcgcg ttggcgatgc tgcaaaaatc ggcgctggta
     3001 ctacccgtat gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga
     3061 ttgtcaactc tggctacttc aaagaaggtt tctccatgca aaccggcacc ggcggcgctt
     3121 cgctggcggt aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact
     3181 tcgccctcgg cggtattacc gcgacgatgg ttgatctgca cgaaaaaggt ctgatccgca
     3241 aactgctgga tgtgcagagc tttgacagcc atgctgcgca atcgctggcc cgtaacccca
     3301 atcacatcga aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc gcatcggttg
     3361 atcgtctcga cgtggtggta ctgagcgcgc tggaaattga caggcagttc aacgttaacg
     3421 tgctgaccgg ttctgacggc gtactgcgtg gcgcttccgg tggtcactgc gataccgcga
     3481 ttgcctctgc gctttccatc atcgtcgcgc cactggtacg cggtcgtatt ccgactctgg
     3541 tggataacgt gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc
     3601 acggtatcgc agttaacccg gcacgtccgg aactagcaga acgtctgcag gaagcgggca
     3661 ttaaagtggt ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac
     3721 agccgattga attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga
     3781 tcgatgttgt gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc
     3841 accatgcggt gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac
     3901 acgcctggct caagcaccat cctgttccac tggtctcctt taccgtggtt gcgcctgggc
     3961 cgattaaaga cagcgaggtc acacgccgaa tttttaatca tcgcgtgaca gccttgcgtg
     4021 ccttagccac aaagcagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg
     4081 ggccggaggg catgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg
     4141 agcttgaaca tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag
     4201 gcgaaattct ctcccgccgc gactattcac tgccgcctcg ccgctgcctg ttgtgcgagc
     4261 aaagcgcagc cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca
     4321 tggaggcact gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg
     4381 acgtcattaa tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat
     4441 ctgtcaccga aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa agatatggcg
     4501 ctggaagatt tccaccgcag cgcgctggcg attcagggct ggttaccccg tttcattgaa
     4561 tttggtgcct gtagtgcaga aatggcaccg gaagcggtac tccacggatt acgcccaatt
     4621 ggtatggctt gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaaggc
     4681 agcatttttt ctttagggct gctatgtgcg gcaattggcc gtttgcttca gctcaaccag
     4741 ccggttacgc caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg cctgaccgat
     4801 cgcgaactgc gtaccaataa ttcacgactg acggcaggtc aacggttgta ccagcagctt
     4861 ggccttaccg gcgcacgcgg tgaagccgaa gcaggttatc cactggtgat caaccacgcc
     4921 ttgccgcatt acctcactct gctggatcag gggttagatc ctgaactggc attgctcgat
     4981 accttgctcc tactgatggc taccaacggc gataccaacg ttgcatcgcg cggtggcgag
     5041 gggggcctgc gctggctaca gcgcgaggcg caaacattat tgcaaaaagg gggcattcga
     5101 acccccaccg atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc
     5161 agtccaggcg gcagtgccga cctgctgatc cttacctggt ttttagcaca gatttaatta
     5221 tttaagcact tgataaattt ggaaatatta attttcggag aacccgtatg tctttagcaa
     5281 aagataatat atggaaacta ttggccccac tggtggtgat gggtgtcatg tttcttatcc
     5341 ctgtccccga cggtatgccg ccgcaggcat ggcattactt cgctgtgttt gtggcaatga
     5401 ttgtcggcat gatcctcgag ccaattccgg caacagcgat cagttttatt gcggttacta
     5461 tttgcgttat tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt
     5521 ttaatgcgca aaaacaggcg ctgaaatggg gcctggctgg tttttccagc accacggtat
     5581 ggctggtatt tggcgcattt atttttgcat tagggtatga agtttccggg ttaggtcgtc
     5641 gcattgccct tttcctggtg aaattcatgg acaaacgcac gctgacgttg ggttatgcga
     5701 ttgtcattat cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg
     5761 gtacggtttt tccggtcatt aaaaacctgc cgccgttgtt taaatcattc ccgaacgatc
     5821 cgtccgcgcg tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga
     5881 gttcgtccat gtttgtcacc ggtgcggacc aaacgtgctg ggtctggagt tcgtcagcaa
     5941 aattgccggt atccagatta gctggttgca gtggttcctc tgcttcctgc cggttggggt
     6001 tatcttgctt atcattgcgc cgtggctttc ctacgtgctg tacaaaccgg aaatcacaca
     6061 cagtgaagaa gtggcaacct gggcgggtga tgaactaaaa accatgggtg cgctgacacg
     6121 cagagagtgg acgctgattg gccttgtatt gctcagctta ggtttgtggg tatttggcag
     6181 tgaagtcatt aatgctactg cggttggtct gctggcagtt tcgctaatgc tggctctgca
     6241 cgttgtgccg tggaaagaca ttacccgcta taacagcgca tggaacacgc tggtcaacct
     6301 ggcaactctg gttgtgatgg ctaacggcct gactcgttct ggttttattg actggttcgc
     6361 cggtaccatg agtacgcacc tggaaggatt ctcaccaaac gcaacggtga ttgtactggt
     6421 tctggtgttc tactttgcac actacctgtt tgccagcctg tctgcgcaca ccgcaaccat
     6481 gctgccggtt attctggccg tcggtaaagg tattccgggc gtaccaatgg aacaactgtg
     6541 tatcctgctg gtgctgtcta tcggtatcat gggctgtctg acgccgtatg caaccggtcc
     6601 tggggtgatt atttacggct gtggctatgt gaaatcaaaa gattactggc gtcttggcgc
     6661 aatcttcggg gtgatttaca tctctatgtt gctgttggtt ggctggccga ttctcgccat
     6721 gtggaactaa tacgttcgcc aaacgctgaa taaatcatca cgcccgccag gtaagcccac
     6781 ctggcgggct tttttatgat ttaatagata gtattcagtg tatgtatgag ttccacaccc
     6841 attatgaaag cattctgccg taacgccgcg ttgctcgcgg tttctctgct tcccttatct
     6901 tctgccaacg ccgtagcgtt gcaggcaaaa cagtatggcg attttgatcg ctatgtcctg
     6961 gccctctcct ggcaaaccgg attttgccag agtcaacacg atcgaaatcg taacgaacga
     7021 gatgaatgtc gcctgcaaac cgaaacgacc aacaaaactg attttctgac cgtacatggt
     7081 ctgtggccag gattgcctaa atcggttgct gcccgtggtg ttgatgaacg ccgctggatg
     7141 cgcttcggtt gcgctactcg cccaatcccg aatctaccag aagcacgcgc cagccgaatg
     7201 tgttcatcgc cggaaaccgg gttatcactg gaaacggccg ctaaactaag tgaagtcatg
     7261 ccaggagctg gcggacgttc ctgcctggaa cgctacgaat atgccaaaca cggtgcctgc
     7321 tttggttttg atccggacgc atacttcggt acgatggtac gcctgaatca agaaattaaa
     7381 gagagcgaag ccggaaaatt ccttgcggat aattacggta aaacagtgag ccgccgtgac
     7441 tttgacgccg cctttgccaa aagctgggga aaagagaacg tgaaagcagt taagctaacg
     7501 tgccagggta accctgcgta tttgactgaa attcagatct cgatcaaagc tgacgccatc
     7561 aacgctccgc tttctgcaaa ctcattcttg ccacaacctc ccccaggtaa ctgtggcaaa
     7621 acctttgtga ttgataaagc gggttattaa tcctctgatt tacaaaacac gctaattcgt
     7681 cttccataac catgccgatt aagaattata cataatgcgc tgcattgagg aattccctga
     7741 atgtgacgca aatcacttca aagagtgagc aacagtcagt atcatcatgt gagttaaacc
     7801 ctcgccgcct gacggtgagg gttttctttt gggtatgttt atctgcgaca ctcgcagtac
     7861 cgacgacatg gagtaaaaat gtccagacca actatcatca ttaacgacct ggatgccgaa
     7921 cgcatcgata ttctgctgga gcaacccgcc tatgctggtt tgccaatcgc cgacgcgtta
     7981 aacgcagagt tggatcgcgc ccaaatgtgt tcgccagaag agatgccaca cgacgtggtg
     8041 acaatgaaca gccgggttaa attccgcaat cttagcgatg gcgaagtgcg tgtgcgcacg
     8101 ctggtgtatc cggcaaaaat gaccgatagc aatactcagc tttccgttat ggctccggta
     8161 ggtgccgcac tgctggggct gcgcgttggc gattccattc actgggaact tccgggcggc
     8221 gttgcaaccc accttgaagt gctggaactc gaataccagc cagaagctgc tggcgactac
     8281 ctgctttaat cccatctgca caagcgataa ctcgctttgc ccagaggatg cactcgcatc
     8341 ctctttacgc aaaatcagca atttttcctg gttctcactg ttcacttttg ctgcaaaatt
     8401 attccgtttc gtttgtggga ataaccatgg cagaagcatt ttacatcctg ataggatttc
     8461 tgattatggc ggcgattatc gtcatggctg tcctttacct cgaaaaccac tcctgacgat
     8521 catattgttc cccccggcat cgcattcacc agacctgaaa ccgccttctg cgccgcctct
     8581 gcgctttgtg caccgggtac cagaatcacc ttacggcact cctcttcacg tttttcgaaa
     8641 atctcatatc cccgggcggc ctcttcaaac ggcatatagt gggtaacaat ttcttctggt
     8701 ttcagtaatc ctttctcaat taacggtaat aattctccca gccatgcgtg aacgtgggtc
     8761 tgtcccattt taaacgtcac ccctttatca aaggcgtcgc caaacaggaa accgtgaata
     8821 aatccagcgt agacgcccgg tacgctaaca atgccgccac gcctgaccgc cgcaatacac
     8881 tggcgcaacg ctttaccgct gctgccctcc agtttcaggt tagtcagcac cgtttccgtg
     8941 gtgctgcctt tcgcttcaaa accgacggcg tctattactg catccacgcc ccggtgacct
     9001 gccgtttgtt caataattga ctgtgccgga tcgctatctt catcgaaatt aatcgggatc
     9061 gcgccgtagc ggtcagcggc gaaacgcaag cggtagggat gatgatcaac aacaaaaatc
     9121 tgttccgcac cgagcaaccg tgcacaggcg attgtcaaca atcccacagg accagcacca
     9181 tagactgcaa cgcttgaacc ttgttggatc tgcacatttt ttgctgcctg ccatgccgtt
     9241 ggcagaatat cagaaaggaa aagggcttta tcatctgaaa gcaaaggcgg tactttaaac
     9301 ggccccacat tccctttcgg gacgcggaca tattccgcct gcccgccagg aacgccacca
     9361 tacaggtgac tataaccaaa caatgccgcg ggagcaggta tctgtttttt attgagcgca
     9421 gcgcctttac ccgcattggt attttcgcag gcggcatatt gctgtaatcg acagaaaaaa
     9481 cagtcgccac aagcaatgac gaacggaatt accacccggt cgcctttttg caaatttttt
     9541 acgtccttcc cggtttcaac gacttccccc ataaattcat gaccaaaaat atcgccatgt
     9601 ttaacctggg gtattttgcc tcgataaaga tggaggtcag agccacagat cgccgttacc
     9661 gtaatacgca gaataatatc atctgcctgt tcaatgcccg gatcgggaac attttctacc
     9721 tgaacgtgat gtgggccgtg ataagtcaat gctttcatgc aacctccaga gacagttcaa
     9781 aaaagagtca gtctttaagg gtagctatca cagcgggaaa acgggcggta ccgcaggttt
     9841 tgggagaatt cctagtgttc agtgagaata tattaatcac agaaaccgtt cgtaaggaaa
     9901 aagaaccaga caaatatcag ggataaatat tataatcatg gagaatgatt tgttcatgat
     9961 taacagggag aaagggttat gtataaggca atcattatgc cagttgatgt atttgaaatg
    10021 gaattgagcg acaaagctgt tcgccacgct gaattcctcg cccaggatga cggagttatt
    10081 catctacttc acgtactacc cgggtcagcc agcctgagcc tgcaccgttt tgccgctgat
    10141 gtgcgtcgtt ttgaagagca tctgcaacat gaagcagaag aacgtctgca aacgatggtc
    10201 agccacttca ccatcgatcc ttcccgcatt aaacaacatg tccgttttgg tagcgtgcgg
    10261 gatgaagtca atgagttggc agaagaactg ggggctgatg ttgtagttat cggttctcgc
    10321 aacccatcga tttcgaccca tctgttaggt tctaacgcct cgagcgtgat ccgccacgcc
    10381 aatctgcctg tactggtcgt tcgctaacag ataaaagtat ttatgccgcc ctgcctggaa
    10441 catcccggca gggcttttta atgccataaa aaagccgcca ggtttgaccc cggcggctaa
    10501 gcaattgcag gtgaatctta cttcttctta tgcagttttg gtgcgaatca ggtagtcaaa
    10561 agcactcaga gaggctttgg caccttcgcc agtggcgatg atgatctgct tgtacggaac
    10621 cgtcgtacag tcacccgctg cgaacacgcc tttgacgttg gtttcacatt tcgcatcaat
    10681 gataatctcg cccatgcggt tacgttcgac tgcgccttcc agccagttgg tgttcggcag
    10741 cagaccaatc tggacaaaaa taccggccag ttcgatgttg tgaatattgc cgctgacacg
    10801 atctcgatat tccagaccaa cgactttgct gccgtcgcct ttcacttccg tggtttgcgc
    10861 attcagaata atgtcgacgt ttttcaggct gcgcagtttg tcctgcagaa cctggtcggc
    10921 tttcatttct ggcgcaaatt ccagcagcgt tacgtgctca acgatacccg ccaggtcgat
    10981 tgccgcttcc acgccggagt taccgccgcc gataaccgct acgcgtttgc ctttaaacag
    11041 cgggccgtcg cagtgcgggc agtaggtcac gcctttagtg cgatactgat cttcgcccgg
    11101 aacgttcatg ttgcgccatt ttgcacccgt cgccacgata atgctgcgtg ctttcagtac
    11161 cgcgccagaa gctgtttcaa tctggtgtag accaccttca accgctgccg ggatcagttt
    11221 gctggcgctc tggctgtcga tcacatcaac gtcgtattca tcaacgtgaa ctttcagtgc
    11281 gcctgccaac ttctgccctt cagtcttcgg tacagaaatg tagttttcga tatcaacggt
    11341 atcgaggatc tgaccaccaa aacgttcgcc catcagaccg gtacggatgc ctttacgtgc
    11401 ggagtaaatt gctgccgctg cacccgccgg gccggaacca acgattaata cgtcataagc
    11461 atcacgcttg ttcagctctt ctgccgcacg tttttccgcg ccagtatcaa ttttggcaac
    11521 gatttcagtc aacgtcatgc ggccctgacc aaactctttc ccgtttacga acactgccgg
    11581 aacgcccatc acgttgcgat cggtgatttc gttctggaag gtgccgccgt caattgcagt
    11641 gtgcttgatg cgcgggttca atacgctcat caggttcagc gcctgcacca cgtccgggca
    11701 gttatggcaa gagagcgagt aataggtttc gaattcaaaa tcaccgtcaa tatggcgaat
    11761 ctgctccagc agagactgcg cttctttcga cggatgacca ccggtccaca gcaacgccag
    11821 taccagcgag gtgaactcgt ggcccagcgg ggagcctgca aaacgtggtc cctggttgga
    11881 acctgggttg gtgatcagga aagacggctt acgcactggc aagctgttat cttctttaaa
    11941 ggtgactttg tctgacagtt ctgcgatttc agccaacagt tccttgattt ctgccgattt
    12001 agcgctgtca tccagcgtgg caattaactc aacaggcttg gtcaatttct caaggtaagc
    12061 cttgagttga gttttcatat ttgtgtcgag catgtttatc tcctgggctt taaacatcat
    12121 catgcaagcg gcctctgcgg ctacctggat gcagcttgca tcatggtgcg gggaagaaaa
    12181 tcgggtgccg cagcacccga agaattaggt gaatttccga agtatttcgc gtcagagcaa
    12241 ggcgactagc ctgtgaatcc ccgggagctt acacaagtaa gtgaccgggg tgagcgggcg
    12301 acgccaacgc cgctatggcg tgaaagacga aggaaattta gattttacca accaggtcca
    12361 gagacggagc cagagttgct tcaccttctt tccatttagc cgggcaaact tcacctgggt
    12421 gagaagctac gtactgagct gctttgattt tacgcagcag atcagacgcg tcacggccaa
    12481 tgccttcagc ggtaacttcg attgcctgga tgataccctg cgggtcaaca acgaaggtcg
    12541 cacggtcagc cagaccttca tcttcacgca tgttgtcgaa gttacgggtc agggcgccag
    12601 tcgggtcgcc gatcatcgca tatttgattt tagcgatggt ttcagagctg ctgtgccatg
    12661 ctttgtgggt gaagtgagta tcggtagata ctgcgtatac gtctacgccc agtttctgca
    12721 gttcttcgta gtggtcagca acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag
    12781 ccgggtagaa gaagaagacg ctccagcggc cttcggtatc tttttcggtg atttcgatga
    12841 attcgccgtt tttgaatgcc tggtttttaa aaggtttaat tttggtgtta atcaaggaca
    12901 tctatacttc ctccgtgttt tcgatgagat gtaaggtaac caatttttgc cgattcggct
    12961 aatgcgtttc cattatcaaa tcgataagtt ttaccttaca accttcgtaa gacaactttg
    13021 tgaactttgc accgccagcg gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg
    13081 taatctgaac taccctttgg tatcttcagc gcctgcctgc aggctgaggt gttgccctta
    13141 caaatgcaac aacgacatgg attacaacac cctcataaac aaagggcaat cacctgatct
    13201 aagctcttac ctatgacagt gataggttat gccttttact cgacttttgc actgactgaa
    13261 aagaacaaat taatgttaaa aaagatactt ttactggctc tgcttcctgc aatcgccttc
    13321 gcagaggaac ttccttcccc agtaaaagcg attgaaaaac agggcattac aatcatcaaa
    13381 acgttcgatg cccccggagg aatgaaaggt tatctcggaa agtatcagga tatgggcgtc
    13441 accatctacc tgactcccga tggtaagcac gctatctctg gttacatgta caacgagaaa
    13501 ggtgaaaacc tgagtaacac acttatcgaa aaagaaattt acgcaccagc cggacgcgaa
    13561 atgtggcaac ggatggaaca atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc
    13621 attgtctacg tcttctccga tccgttctgc ccatattgta aacagttctg gcagcaggcg
    13681 cgcccgtggg tagattctgg caaagtgcaa ttaagaacat tgttggttgg ggttatcaag
    13741 ccagaaagcc cggcgacagc agcggcaatt cttgcctcca aagatcccgc aaaaacctgg
    13801 caacaatatg aagcctctgg ttgcaagctt aagctaaacg tgcctgcaaa cgtaagtaca
    13861 gagcaaatga aagtgttaag tgacaatgag aaactgatgg acgatctggg ggcaaatgtc
    13921 acgccggcta tctattacat gagtaaggaa aatacgctgc aacaggccgt ggggttgccc
    13981 gatcagaaaa cgcttaatat cattatggga aataaataaa aggtaattca tggatggttg
    14041 aactatatcc ctgcttttca accatcctaa aaaagcatga atttctcgct ctaccctaag
    14101 tggctgtgct attttagaaa aacttaatcc tgagttaagc caaaaaacat aatcaataaa
    14161 aatacatacg caaaaaacat tgattaagtg aatataccat ggaagaaaaa tataaaccgg
    14221 agtagtgtat ggccaatctc tacgacttga aaaagttcga cttaaatctt cttgtcatat
    14281 ttgagtgtat ttaccagcat ttaagcatta gcaaagctgc tgaatcacta tatatcaccc
    14341 catcagccgt gagccagtcg ttacaacgct tacgtgcgca atttaatgac cctttattca
    14401 ttcgctcagg aaaaggtatc gcgccgacga caacagggtt gaatctgcat caccatcttg
    14461 agaaaaacct caaaggcctt gaacaaacaa ttaatatagt caataagtcc gaactaaaga
    14521 aaaactttat tatctacggc cctcagctca tttcttgctc taataatagt atgctgatcc
    14581 gctgcctacg gcaagatgcc tctgtcgaga ttgagtgcca tgatatcctc atgtcagcag
    14641 agaatgctga agaactgttg gttcaccgca aagccgatct ggtgatcacc caaatgccag
    14701 tgatcagccg ttccgtcatt tgcatgccat tgcatacaat tcgcaatact ctaatttgta
    14761 gtaataagca tccgcgaatt actgacaata gcacttatga acaaattatg gcagaagagt
    14821 tcacccaatt aatagccaaa tcagcaggag ttgatgatat tcaaatggaa atagacgaga
    14881 agtttatgaa tcgcaaaatt tcatttcgtg gttcctcttt gctgacaata attaatagca
    14941 ttgcagttac agatttactg ggaatagtcc cctatgaact ttataactac caccgtgact
    15001 ttcttaattt aaaagagata aaactagaat atccgttacc ttcaataaag ctttacattt
    15061 cctataataa atcttcttta aataatttgg tgttctcccg atttattgac cgtctaaatg
    15121 agagtttttg atatataact atagacagct actataactt tcatctattt attcacagcg
    15181 cgaatgaaaa ataaaacact ctgctttaca gtgtttactt tataattaac agtcttatct
    15241 cactgttcac cctgtaacgt tcagagagtg tcttcagatg tctatttata aaattcctct
    15301 tccgctcaat attctggaag ctgcacgaga gcgtattacc tagacactca acaccctacc
    15361 ccgcgtttgt gtctcatttt ctggcggcaa agactctggt ttaatgctgc atctgacagc
    15421 cgaacttgcc cgacaaatgg gcaaaaaaat ctgcgttttg tttatcaact gggaggc
//
LOCUS       DAJVPP010000090        15027 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVPP010000090 DAJVPP010000000
VERSION     DAJVPP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.090"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OQM34_002999"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OQM34_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCS1453354.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OQM34_003000"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OQM34_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCS1453355.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OQM34_003001"
     CDS             complement(941..1408)
                     /locus_tag="OQM34_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1453356.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1789..2100
                     /gene="rpsJ"
                     /locus_tag="OQM34_003002"
     CDS             1789..2100
                     /gene="rpsJ"
                     /locus_tag="OQM34_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1453357.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2133..2762
                     /gene="rplC"
                     /locus_tag="OQM34_003003"
     CDS             2133..2762
                     /gene="rplC"
                     /locus_tag="OQM34_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1453358.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2773..3378
                     /gene="rplD"
                     /locus_tag="OQM34_003004"
     CDS             2773..3378
                     /gene="rplD"
                     /locus_tag="OQM34_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1453359.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3375..3677
                     /gene="rplW"
                     /locus_tag="OQM34_003005"
     CDS             3375..3677
                     /gene="rplW"
                     /locus_tag="OQM34_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1453360.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3695..4516
                     /gene="rplB"
                     /locus_tag="OQM34_003006"
     CDS             3695..4516
                     /gene="rplB"
                     /locus_tag="OQM34_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1453361.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4533..4811
                     /gene="rpsS"
                     /locus_tag="OQM34_003007"
     CDS             4533..4811
                     /gene="rpsS"
                     /locus_tag="OQM34_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1453362.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4826..5158
                     /gene="rplV"
                     /locus_tag="OQM34_003008"
     CDS             4826..5158
                     /gene="rplV"
                     /locus_tag="OQM34_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1453363.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5176..5877
                     /gene="rpsC"
                     /locus_tag="OQM34_003009"
     CDS             5176..5877
                     /gene="rpsC"
                     /locus_tag="OQM34_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1453364.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5890..6300
                     /gene="rplP"
                     /locus_tag="OQM34_003010"
     CDS             5890..6300
                     /gene="rplP"
                     /locus_tag="OQM34_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1453365.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6300..6491
                     /gene="rpmC"
                     /locus_tag="OQM34_003011"
     CDS             6300..6491
                     /gene="rpmC"
                     /locus_tag="OQM34_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1453366.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6491..6745
                     /gene="rpsQ"
                     /locus_tag="OQM34_003012"
     CDS             6491..6745
                     /gene="rpsQ"
                     /locus_tag="OQM34_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1453367.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6910..7281
                     /gene="rplN"
                     /locus_tag="OQM34_003013"
     CDS             6910..7281
                     /gene="rplN"
                     /locus_tag="OQM34_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1453368.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7292..7606
                     /gene="rplX"
                     /locus_tag="OQM34_003014"
     CDS             7292..7606
                     /gene="rplX"
                     /locus_tag="OQM34_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1453369.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7621..8160
                     /gene="rplE"
                     /locus_tag="OQM34_003015"
     CDS             7621..8160
                     /gene="rplE"
                     /locus_tag="OQM34_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1453370.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8175..8480
                     /gene="rpsN"
                     /locus_tag="OQM34_003016"
     CDS             8175..8480
                     /gene="rpsN"
                     /locus_tag="OQM34_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1453371.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8514..8906
                     /gene="rpsH"
                     /locus_tag="OQM34_003017"
     CDS             8514..8906
                     /gene="rpsH"
                     /locus_tag="OQM34_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1453372.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8919..9452
                     /gene="rplF"
                     /locus_tag="OQM34_003018"
     CDS             8919..9452
                     /gene="rplF"
                     /locus_tag="OQM34_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1453373.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9462..9815
                     /gene="rplR"
                     /locus_tag="OQM34_003019"
     CDS             9462..9815
                     /gene="rplR"
                     /locus_tag="OQM34_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1453374.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9830..10333
                     /gene="rpsE"
                     /locus_tag="OQM34_003020"
     CDS             9830..10333
                     /gene="rpsE"
                     /locus_tag="OQM34_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1453375.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10337..10516
                     /gene="rpmD"
                     /locus_tag="OQM34_003021"
     CDS             10337..10516
                     /gene="rpmD"
                     /locus_tag="OQM34_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1453376.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10520..10954
                     /gene="rplO"
                     /locus_tag="OQM34_003022"
     CDS             10520..10954
                     /gene="rplO"
                     /locus_tag="OQM34_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1453377.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10962..12293
                     /gene="secY"
                     /locus_tag="OQM34_003023"
     CDS             10962..12293
                     /gene="secY"
                     /locus_tag="OQM34_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1453378.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12325..12441
                     /gene="rpmJ"
                     /locus_tag="OQM34_003024"
     CDS             12325..12441
                     /gene="rpmJ"
                     /locus_tag="OQM34_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1453379.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12588..12944
                     /gene="rpsM"
                     /locus_tag="OQM34_003025"
     CDS             12588..12944
                     /gene="rpsM"
                     /locus_tag="OQM34_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1453380.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12961..13350
                     /gene="rpsK"
                     /locus_tag="OQM34_003026"
     CDS             12961..13350
                     /gene="rpsK"
                     /locus_tag="OQM34_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1453381.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13384..14004
                     /gene="rpsD"
                     /locus_tag="OQM34_003027"
     CDS             13384..14004
                     /gene="rpsD"
                     /locus_tag="OQM34_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1453382.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14030..15019
                     /gene="rpoA"
                     /locus_tag="OQM34_003028"
     CDS             14030..15019
                     /gene="rpoA"
                     /locus_tag="OQM34_003028"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1453383.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
BASE COUNT         3885 a         3518 c         4000 g         3624 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccttttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agagcgctga tttgttaacc tgattccggt
     1501 gaaaaacgct atcttcacaa ccttacaaat ttcgtggaat gcagctcgca atctgcgcgt
     1561 tgcgattaat ttctacccga tgcttgcgtc aggctcagat ttgcgtataa tgcgcgggct
     1621 tgtcgtagtt gacagcaggt tcaatctgaa cctcagttag ccgaatttgg ctacctaaca
     1681 atgctcccaa tcggggagct acgtaagaac ggttacactc tcccatcaat cgtaatgggt
     1741 ctgaggagta atcattttcg tttataaaat aattggagct ctggtctcat gcagaaccaa
     1801 agaatccgta tccgcctgaa agcgtttgat catcgtctga tcgatcaagc aaccgcggaa
     1861 atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg gtccgatccc gctgccgaca
     1921 cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca acaaagacgc gcgcgatcag
     1981 tacgaaatcc gtactcactt gcgtctggtt gacatcgttg agccaaccga gaaaaccgtt
     2041 gatgctctga tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag cctgggttaa
     2101 tcaggtcatt gagcgattga gaggttgaaa caatgattgg tttagtcggt aaaaaagtgg
     2161 gtatgacccg tatcttcaca gaagacggcg tttctatccc agtaaccgta atcgaagttg
     2221 aagcaaaccg cgttactcag gttaaagacc tggctaacga tggctaccgt gctattcagg
     2281 tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc cacttcgcta
     2341 aagctggcgt agaagctggc cgtggtctgt gggaattccg cctggctgaa ggcgaagagt
     2401 tcactgtagg tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa gttgacgtaa
     2461 ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac ttccgtaccc
     2521 aggacgctac tcacggtaac tccttgtctc accgcgttcc gggttctatc ggtcagaacc
     2581 agactccggg caaagtgttc aaaggcaaga aaatggcagg tcagatgggt aacgaacgtg
     2641 taaccgttca gagccttgac gtagtacgcg ttgacgctga gcgcaacctg ctgctggtta
     2701 aaggtgctgt cccgggtgca accggtagcg acctgatcgt taaaccagct gtgaaggcgt
     2761 aaggagatag caatggaatt agtattgaaa gacgcgcaga gcgcgctgac tgtttccgaa
     2821 actaccttcg gtcgtgattt caacgaagcg ctggttcacc aggttgttgt tgcttatgca
     2881 gctggtgctc gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac tggttccggt
     2941 aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat caagagcccg
     3001 atctggcgtt ctggtggcgt gacctttgct gctcgtccgc aggaccacag tcaaaaagtt
     3061 aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt acgtcaggat
     3121 cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga aaactaagct gctggcacag
     3181 aaactgaaag acatggctct ggaagatgtg ctgatcatca ccggtgagct ggacgaaaac
     3241 ctgttcctgg ctgcgcgcaa cctgcagaag gttgacgtac gcgatgcaac tggtatcgac
     3301 ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt taagcaagtt
     3361 gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt gcaccgcacg
     3421 tttctgaaaa agcgtctact gcgatggaaa aatccaacac catcgtactc aaagttgcta
     3481 aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa gtcgaagtcg
     3541 aagtcgttaa caccctggta gttaaaggga aagttaaacg tcacggacag cgtatcggtc
     3601 gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat ctggacttcg
     3661 ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca gttgttaaat gtaaaccgac
     3721 atctccgggt cgtcgccacg tagttaaagt ggttaaccct gagctgcaca agggcaaacc
     3781 ttttgctccg ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca atggccgtat
     3841 caccactcgt catatcggtg gtggccacaa gcaggcttac cgtattgttg acttcaaacg
     3901 caacaaagac ggtatcccgg cagttgttga acgtcttgag tacgatccga accgttccgc
     3961 gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt tacatcctgg cccctaaagg
     4021 cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac caggtaacac
     4081 cctgccgatg cgcaacatcc cggttggttc tactgttcat aacgtagaaa tgaaaccagg
     4141 taaaggcggt cagctggcac gttccgctgg tacttacgtt cagatcgttg ctcgtgatgg
     4201 tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag cagactgccg
     4261 tgcaactctg ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg gtaaagcagg
     4321 tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga acccggtaga
     4381 ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa ctccgtgggg
     4441 cgttcagacc aaaggtaaga agacccgcag caacaagcgt actgataaat tcatcgtacg
     4501 tcgccgtagc aaataatttt agaggataag ccatgccacg ttctctcaag aaaggtcctt
     4561 ttattgacct gcacttgctg aagaaggtag agaaagcggt ggaaagcgga gacaagaagc
     4621 ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa catgatcggt ttgaccatcg
     4681 ctgtccataa tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg gtcggtcaca
     4741 aactgggtga attcgcaccg actcgtactt atcgcggcca cgctgctgat aaaaaagcga
     4801 agaagaaata aggtaggagg aagagatgga aactattgct aaacatcgcc atgctcgttc
     4861 ttctgctcag aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag tgtcgcaggc
     4921 tctggatatt ttgacctaca ccaacaagaa agcggctgta caggtcaaga aagttctgga
     4981 atctgccatt gctaacgctg aacacaacga tggcgctgac attgacgatc tgaaagttac
     5041 gaaaattttc gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg caaaaggtcg
     5101 tgcagatcgc atcctgaagc gcaccagcca catcactgtg gttgtgtccg atcgctgaga
     5161 ctctggagac tagcaatggg tcagaaagta catcctaatg gtattcgctt gggtattgta
     5221 aaaccatgga actctacttg gtttgcgaac accaaagaat tcgctgacaa cctggacagc
     5281 gattttaaag tacgtcagta cctgactaag gaactggcta aagcgtccgt atctcgtatc
     5341 gttatcgagc gtccggctaa gagcatccgt gtaaccattc acactgctcg cccgggtatc
     5401 gttatcggta aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc ggacatcgct
     5461 ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc ctgaactgga cgcaaaactg
     5521 gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg tgctatgaag
     5581 cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta ttaaagttga agttagcggc
     5641 cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg cgtaccgctg
     5701 cacactctgc gtgctgacat cgactacaac acctctgaag cgcacaccac ttacggtgta
     5761 atcggcgtta aagtgtggat cttcaaaggc gagatcctgg gtggtatggc tgctgttgaa
     5821 caaccggaaa aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg taaataagga
     5881 gcgtcgctga tgttacaacc aaagcgtaca aaattccgta aaatgcacaa aggccgtaac
     5941 cgcggtctgg cgcagggtac ggatgttagc ttcggcagct tcggtctgaa agctgttggc
     6001 cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac ccgtgcagtt
     6061 aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca aaccgatcac tgaaaagccg
     6121 ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt attgggttgc cttgattcag
     6181 ccgggtaaag tcctgtatga aatggacggt gttccggaag agctggcccg tgaagcattc
     6241 aagctggcag cagcgaaact gccgattaaa accacctttg taactaagac ggtgatgtaa
     6301 tgaaagcaaa agagctgcgt gagaagagca ttgaagagct gaacaccgag ctgctgaacc
     6361 tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag tggccagctg caacagtctc
     6421 acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg aacgagaagg
     6481 cgggtgcgta atgaccgata aaatccgtac tctgcaaggt cgcgttgtta gcgacaaaat
     6541 ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa cacccgatct acggtaaatt
     6601 catcaagcgt acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg gtatcggtga
     6661 cgtggttgaa atccgcgaat gccgtccgct gtccaagact aaatcctgga cgctggttcg
     6721 cgttgtagag aaagcggttc tgtaatacag tacactctct cgatacgaat aaacggctca
     6781 gaaatgagcc gtttattttt tctacccata tccttgaagc ggtgttataa tgccgcgccc
     6841 tcgatatggg gatttttaac gacctgattt tcgggtctca gtagtagttg acattagcgg
     6901 agcactaaaa tgatccaaga acagactatg ctgaacgtcg ccgacaactc cggtgcacgt
     6961 cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg cgtaggcgac
     7021 atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa aggtgatgtg
     7081 ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg ttctgtcatt
     7141 cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc tatcggtacg
     7201 cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa aattatctct
     7261 ctggcaccag aagtactcta aggagcgaat catggcagcg aaaatccgtc gtgatgacga
     7321 agttatcgtg ttaaccggta aagataaagg taaacgcggt aaagttaaga atgtcctgtc
     7381 ttccggcaag gtcattgttg aaggtatcaa cctggttaag aaacatcaga agccggttcc
     7441 ggccctgaac caaccgggtg gcatcgttga aaaagaagcc gctattcagg tttccaacgt
     7501 agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat tcgaagacgg
     7561 caaaaaagtc cgtttcttca agtctaacag cgaaactatc aagtaatttg gagtagtacg
     7621 atggcgaaac tgcatgatta ctacaaagac gaagtagtta aaaaactcat gactgagttt
     7681 aactacaatt ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa catgggtgtt
     7741 ggtgaagcga tcgctgacaa aaaactgctg gataacgcag cagcagacct agcagcaatc
     7801 tccggtcaaa aaccgctgat caccaaagca cgcaaatctg ttgcaggctt caaaatccgt
     7861 cagggctatc cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg ggagttcttt
     7921 gagcgcctga tcactattgc tgtacctcgt atccgtgact tccgtggcct gtccgctaag
     7981 tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat cttcccagaa
     8041 atcgactacg ataaagtcga ccgcgttcgt ggtttggata ttaccattac cactactgcg
     8101 aaatctgacg aagaaggccg cgctctgctg gctgcctttg acttcccgtt ccgcaagtaa
     8161 ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa cgcgtagctt
     8221 tagctgataa atacttcgcg aaacgcgctg aactgaaagc gatcatctct gatgtgaacg
     8281 cttccgacga agatcgttgg aacgctgttc tcaagctgca gactctgccg cgtgattcca
     8341 gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt ttcctgcgga
     8401 agttcgggtt gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa atcccgggtc
     8461 tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga cagatgagca
     8521 tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc gcgaacaaag
     8581 ctgcggtcac catgccttcc tccaagctga aagtggcaat cgccaacgtg ctgaaggaag
     8641 aaggttttat tgaagatttt aaagttgaag gcgacaccaa gcctgaactg gaacttactc
     8701 tgaagtattt ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc cgcccaggtc
     8761 tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg ggtatcgcag
     8821 ttgtttctac ctctaaaggt gttatgactg atcgtgcagc gcgccaggct ggtcttggtg
     8881 gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt gctaaagcac
     8941 cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt acgatcaaag
     9001 gtaaaaacgg cgagctgact cgtactctca acgatgctgt tgaagttaaa catgcagata
     9061 ataccctgac cttcggtccg cgtgatggtt acgcagacgg ttgggcacag gctggtaccg
     9121 cgcgtgccct gctgaactca atggttatcg gtgttaccga aggcttcact aagaagctgc
     9181 agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac ctgtctctgg
     9241 gtttctctca tcctgttgac catcagctgc ctgcgggtat cactgctgaa tgtccgactc
     9301 agactgaaat cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt gcagcggatc
     9361 tgcgcgccta ccgtcgtcct gagccttaca aaggcaaggg tgttcgttac gccgacgaag
     9421 tcgtgcgtac caaagaggct aagaagaagt aaggtaacac tatggataag aaatctgctc
     9481 gtatccgtcg tgcgacccgc gcacgccgca agctccagga gctgggcgca actcgcctgg
     9541 tggtacatcg taccccgcgt cacatttacg cacaggtaat tgcaccgaac ggttctgaag
     9601 ttctggtagc tgcttctact gtagaaaaag ctatcgctga acaactgaag tacaccggta
     9661 acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg gaaaaaggca
     9721 tcaaagatgt atcctttgac cgttccgggt tccaatatca tggtcgtgtc caggcactgg
     9781 cagatgctgc ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga tggctcacat
     9841 cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg tatctaaaac
     9901 cgttaaaggt ggtcgtattt tctccttcac agctctgact gtagttggcg atggtaacgg
     9961 tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc agaaagcgat
    10021 ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat aacggcactc tacaacaccc
    10081 tgttaaaggt gttcacacgg gttctcgcgt attcatgcag ccggcttccg aaggtaccgg
    10141 tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc ataacgttct
    10201 ggctaaagca tatggttcca ccaacccgat caacgtggtt cgtgcaacta ttgatggcct
    10261 ggaaaatatg aattctccag aaatggtcgc tgccaagcgt ggtaaatccg ttgaagaaat
    10321 tctggggaaa taaaccatgg caaagactat taaaattact caaacccgca gtgcaatcgg
    10381 tcgtctgccg aaacacaagg caacgctgct tggcttgggt ctgcgtcgta ttggtcacac
    10441 cgtagagcgc gaggatactc ctgctattcg cggtatgatc aacgcggttt ccttcatggt
    10501 taaagttgag gagtaagaga tgcgtttaaa tactctgtct ccggccgaag gctccaaaaa
    10561 ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg gtggtcgtgg
    10621 tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg agggtggtca
    10681 gatgcctctg taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag cagcgattac
    10741 agccgaagtt cgtctatctg acctggctaa agtagaaggc ggtgttgtag acctgaacac
    10801 gctgaaagcg gctaacatta tcggtatcca gatcgagttc gcgaaagtga tcctggctgg
    10861 cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg ctcgtgctgc
    10921 tatcgaagct gctggcggta aaatcgagga ataagtagca gatggctaaa caaccgggat
    10981 tagattttca aagtgccaaa ggtggcttag gcgagctgaa acgcagactg ctgtttgtta
    11041 tcggtgcgct gattgtgttc cgtattggct cttttattcc gatccctggt attgatgccg
    11101 ctgtacttgc caaactgctt gagcaacagc gaggcaccat cattgagatg tttaacatgt
    11161 tctctggtgg tgctctcagc cgtgcttcta tctttgctct ggggatcatg ccgtatattt
    11221 cggcgtcgat cattatccag ctgctgacgg tggttcaccc aacgttggca gaaattaaga
    11281 aagaagggga gtctggtcgt cgtaagatca gccagtacac ccgttacggt actctggtgc
    11341 tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct ggtatgcaag
    11401 gcctggtgat taacccgggc tttgcattct acttcaccgc tgttgtaagt ctggtcacag
    11461 gaacgatgtt cctgatgtgg ttgggcgaac agatcactga acgaggtatc ggcaacggta
    11521 tttcaatcat tatcttcgcc ggtattgtcg cgggactccc gccagccatt gcccatacta
    11581 tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt gcagtattag
    11641 tatttgcagt gacgttcttt gttgtgtttg ttgagcgtgg tcaacgccgc attgtggtaa
    11701 actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca catttaccgc
    11761 tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc ttccagtatt attctgttcc
    11821 cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg gaactggctg acaacaattt
    11881 cgctgtattt gcagcctggg caaccgcttt atgtgttact ctatgcgtct gcaatcatct
    11941 tcttctgttt cttctacacg gcgttggttt tcaacccgcg tgaaacagca gataacctga
    12001 agaagtccgg tgcatttgta ccaggaattc gtccgggaga gcaaacggcg aagtatatcg
    12061 ataaagtaat gacccgcctg accttggttg gtgcgctgta tattaccttt atctgcctga
    12121 tcccggagtt catgcgtgat gcaatgaaag taccgttcta cttcggtggg acctcactgc
    12181 ttatcgttgt tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca
    12241 gtcagtatga gtctgcattg aagaaggcga acctgaaagg ctacggccga taattggtcg
    12301 cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt
    12361 aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag
    12421 cataaacagc gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg
    12481 gctagattag ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa
    12541 cgggcttttc agcatggaac gtacatatta aatagtagga gtgcatagtg gcccgtatag
    12601 caggcattaa cattcctgat cataagcatg ccgtaatcgc attaacttcg atttatggcg
    12661 tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa gatgttaaga
    12721 tcagtgagct gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc aaatttgtcg
    12781 ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg gatcttggtt
    12841 gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt accaagacca
    12901 acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg tgattgaata
    12961 atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc tgacggcgtg
    13021 gctcatatcc atgcttcttt caacaacacc atcgtgacta tcactgatcg tcagggtaac
    13081 gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa atccactccg
    13141 tttgcagctc aggttgcagc agagcgttgc gctgacgccg tgaaagaata cggcatcaag
    13201 aatctggaag ttatggttaa aggtccgggt ccaggccgcg aatctactat tcgtgctctg
    13261 aacgccgcag gtttccgcat cactaacatt actgatgtga ctccgatccc tcataacggt
    13321 tgtcgtccgc cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg ttggagaaag
    13381 aaaatggcaa gatatttggg tcctaagctc aagctgagcc gtcgtgaggg caccgactta
    13441 ttccttaagt ctggcgttcg cgcgatcgat accaagtgta aaattgaaca agctcctggc
    13501 cagcacggtg cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg tgaaaagcaa
    13561 aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc gtaactacta caaagaagca
    13621 gcacgtctga aaggcaacac cggtgaaaac ctgttggctc tgctggaagg tcgtctggac
    13681 aacgttgtat accgtatggg cttcggtgcc actcgtgcag aagcacgtca gctggttagc
    13741 cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca ggttagtccg
    13801 aatgacgttg taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa agccgctctg
    13861 gagctggctg agcagcgtga aaagccaact tggctggaag ttgatgctgg caagatggaa
    13921 ggtacgttta agcgtaagcc ggagcgttct gatctgtctg cggacattaa cgaacacctg
    13981 atcgtcgagc tttactccaa gtaaagctta gtaccaaaga gaggacacaa tgcagggttc
    14041 tgtgacagag tttctaaaac cgcgcctggt tgatatcgag caagtgagtt cgacgcacgc
    14101 caaggtgacc cttgagcctt tagagcgtgg ctttggccat actctgggta acgcactgcg
    14161 ccgtattctg ctctcatcga tgccgggttg cgcggtgacc gaggttgaga ttgatggtgt
    14221 actacatgag tacagcacca aagaaggcgt tcaggaagat atcctggaaa tcctgctcaa
    14281 cctgaaaggg ctggcggtga gagttcaggg caaagatgaa gttattctta ccttgaataa
    14341 atctggcatt ggccctgtga ctgcagccga tatcacccac gacggtgatg tcgaaatcgt
    14401 caagccgcag cacgtgatct gccacctgac cgatgagaac gcgtctatta gcatgcgtat
    14461 caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt cggaagaaga
    14521 tgagcgccca atcggccgtc tgctggtcga cgcatgctac agccctgtgg agcgtattgc
    14581 ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc tggtcatcga
    14641 aatggaaacc aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg caaccattct
    14701 ggctgaacaa ctggaagctt tcgttgactt acgtgatgta cgtcagcctg aagtgaaaga
    14761 agagaaacca gagttcgatc cgatcctgct gcgccctgtt gacgatctgg aattgactgt
    14821 ccgctctgct aactgcctta aagcagaagc tatccactat atcggtgatc tggtacagcg
    14881 taccgaggtt gagctcctta aaacgcctaa ccttggtaaa aaatctctta ctgagattaa
    14941 agacgtgttg gcttcccgtg gactgtctct gggcatgcgc ttggaaaact ggccaccggc
    15001 aagcatcgct gacgagtaac cggatca
//
LOCUS       DAJVPP010000091        14962 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVPP010000091 DAJVPP010000000
VERSION     DAJVPP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.091"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..816)
                     /gene="tdh"
                     /locus_tag="OQM34_003029"
     CDS             complement(<1..816)
                     /gene="tdh"
                     /locus_tag="OQM34_003029"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1453384.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSID"
     gene            complement(826..2022)
                     /gene="kbl"
                     /locus_tag="OQM34_003030"
     CDS             complement(826..2022)
                     /gene="kbl"
                     /locus_tag="OQM34_003030"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1453385.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2297..3154)
                     /gene="yibB"
                     /locus_tag="OQM34_003031"
     CDS             complement(2297..3154)
                     /gene="yibB"
                     /locus_tag="OQM34_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCS1453386.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3458..4390
                     /gene="rfaD"
                     /locus_tag="OQM34_003032"
     CDS             3458..4390
                     /gene="rfaD"
                     /locus_tag="OQM34_003032"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1453387.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4400..5446
                     /gene="rfaF"
                     /locus_tag="OQM34_003033"
     CDS             4400..5446
                     /gene="rfaF"
                     /locus_tag="OQM34_003033"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1453388.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5450..6442
                     /gene="rfaC"
                     /locus_tag="OQM34_003034"
     CDS             5450..6442
                     /gene="rfaC"
                     /locus_tag="OQM34_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1453389.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYTIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6439..7647
                     /gene="rfaL"
                     /locus_tag="OQM34_003035"
     CDS             6439..7647
                     /gene="rfaL"
                     /locus_tag="OQM34_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCS1453390.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(7683..8825)
                     /locus_tag="OQM34_003036"
     CDS             complement(7683..8825)
                     /locus_tag="OQM34_003036"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCS1453391.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(8834..9847)
                     /locus_tag="OQM34_003037"
     CDS             complement(8834..9847)
                     /locus_tag="OQM34_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCS1453392.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(9872..10579)
                     /gene="rfaY"
                     /locus_tag="OQM34_003038"
     CDS             complement(9872..10579)
                     /gene="rfaY"
                     /locus_tag="OQM34_003038"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1453393.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10605..11612)
                     /gene="waaO"
                     /locus_tag="OQM34_003039"
                     /gene_synonym="rfaI"
     CDS             complement(10605..11612)
                     /gene="waaO"
                     /locus_tag="OQM34_003039"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1453394.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(11655..12452)
                     /gene="rfaP"
                     /locus_tag="OQM34_003040"
     CDS             complement(11655..12452)
                     /gene="rfaP"
                     /locus_tag="OQM34_003040"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1453395.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12445..13569)
                     /locus_tag="OQM34_003041"
     CDS             complement(12445..13569)
                     /locus_tag="OQM34_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1453396.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13566..14624)
                     /gene="rfaQ"
                     /locus_tag="OQM34_003042"
     CDS             complement(13566..14624)
                     /gene="rfaQ"
                     /locus_tag="OQM34_003042"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1453397.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4258 a         3364 c         3063 g         4277 t
ORIGIN      
        1 gtcgatagac atatcagacg gtggaatacc cagcatcgca atacgaccgc cgtggttcat
       61 ggtatcgagc atggtacgaa acgctggcgg cgcgccggac atttccagac cgacatcaaa
      121 gccttcggtc atgcccagtt cagccatcac atcattaagg ttttctttcg cgacgttaac
      181 cgcacgggtg atacccattt tgcgcgccag ctcaaggcgg tattcgttaa catcggtgat
      241 caccacattg cgtgcaccaa cgtgtttcgc caccgccgca gccataatac ctatcggacc
      301 tgcaccagaa accagcacat cttcgcccac cagatcgaac gacagcgcgg tatgcacggc
      361 gttaccgaag ggatcaaaaa tggaagccag gtcgtcggag atattgtcgg ggattttgaa
      421 agcgttgaac gccggaatca ccagatattc ggcgaagcag cccggacggt taacaccgac
      481 accaatggtg ttgcggcaca gatgggtacg accgccacga cagttgcgac agtgaccgca
      541 ggtgatatgg ccttcgccag aaacgcgatc gccgattttg aagcctttca cttcctgacc
      601 aatacctacc acttcaccga catattcatg gcccacgacc atcggcaccg ggatggtttt
      661 ttgcgaccac tcatcccagt tatagatgtg aacgtcagtc ccgcagatgg ctgttttacg
      721 gattttaatc agcagatcgt tatgcccgag ttccggtaca gggacgtcgg tcatccagat
      781 gccctcttcc gctttcagtt tggataacgc tttcatctca catcctcagg cgataacgcc
      841 cagttgttta ccaatacgcg tgaatgcttc tactgcacgc gtaatttgct caggggtatg
      901 cgccgcagac atctgggtac gaatacgcgc ctgacctttc ggaacgaccg gatagaagaa
      961 accggtgacg taaatgccct ctttttgcag ctctcgggca aatttctgcg ccactaccgc
     1021 atcaccaagc atgaccggaa taatggcgtg atcggctccc gccagggtaa agcccgccgc
     1081 cgacatttgc tcacggaact gacgcgcgtt cgcccacaga cggtcacgca actcgctacc
     1141 cgcttcaacc atctccagca ctttgatgga agcggcgaca attgctggtg ccagcgagtt
     1201 ggagaacagg tacggacgag aacgctgccg cagccactca accacttctt tgcgcgccgc
     1261 ggtataacca ccagaagccc cgcccagcgc tttaccgagc gtgccggtga tgatgtcgac
     1321 acgccccatc acatcgcagt attcatggga accacgacca ttttcaccga caaaaccgac
     1381 cgcgtgggag tcgtctacca tcaccagggc atcgtattta tctgccagat cgcaaacgcc
     1441 cttcagattg gcaatcacgc cgtccatcga gaacacacca tcggtggcaa tcagcacatg
     1501 acgcgcaccg gcttcacgcg cttctttcag acgagcttca agctcctgca tatcgttgtt
     1561 ggcatagcga tagcgtttag ctttacacag acgcacacca tcaataatag acgcgtggtt
     1621 cagtgcgtcg gagataatgg cgtcttccgc gcccagcagc gtttcaaaca ggccaccgtt
     1681 agcatcaaag caggaagagt agagaatcgc atcttccatc cccaggaatg ccgccagttt
     1741 ttgttcaagc tctttatggc tgtcctgagt gccgcaaata aaacgcaccg aagccatgcc
     1801 gaaaccgtga gaatccattc ccgcctttgc tgccgcaatc agatccggat gattcgccag
     1861 cccgagatag ttgttggcac aaaagttaat gacgtggctt ccatcagcca cggtgatatc
     1921 tgcttgctgc gcagacgtaa taatgcgctc ttctttaaac aacccttccg cccgtgcggt
     1981 ttccagatcg ttggttaact gctgataaaa ttctccacgc attgcgattc tccagacagg
     2041 gcaaattcca gcacatatta cccaaactta taggtcgcga cgagatgatg cgttaacact
     2101 tctgcaaaat tcaggataaa taacgtgtat cttgatgtct ctcaacacct acagatagta
     2161 gaaaaactgt acagttttgg ctgagcataa tgatgagtga ttttactgca ttgatataaa
     2221 aagaaaaaat gatgccaaag catcattttt cctatctcag ttggcatcaa aacgctaccc
     2281 actaatggag ggaaacctat tttcttgaaa ggaaaattct cagcgcctgc atttttgccc
     2341 ctaaagtgtt actcctgaag cagcgaaaca aatcgaacca ttttcctctc cccagatagt
     2401 ttaggttgaa gagatcggaa cgtttgtaat agcacatgac aaaaatgccc tggtcatcgt
     2461 ccacaatgtt attgtttagt gttatttttt gactttccag aactaattta tagaattctt
     2521 tccatttatg ctgtgaacca acaattgcgc ccccaataat atatacatga ttgccaatca
     2581 tgaaatcaaa agcctgttgt tgagatgtaa cagtaagccc tttttttata gtgaaaagat
     2641 gcatcttatt ttcatcaaat gggaaatccc aaattttcaa tcctcgagtt acatttggct
     2701 tacggcaata gccaaaatct atccacgcaa caagaggcgt tttcactaaa cccatgttga
     2761 tagctttatt tacaaaatag gccttaaggt tacataccaa aacatactct ggtgaccagt
     2821 actctgggtt ttttaattgt cgaggctcaa gtctatttgt aaatgactca tctttttgaa
     2881 ttttttcgat acgacttctg atatatctaa atttcttttt tatatcgata acgataaccg
     2941 ttgttggttt tccgttgcga atcgcttcaa tccttggttt caaatcggga gaagtgaaaa
     3001 taatcatctc attttcaagt gcggccagac gctcaaagta actaaaataa acatcaactg
     3061 aacgagctag tttttcacgg aaccctttat tagcagtcca atcccctcta ccaatgtcaa
     3121 aatatgcagt gataattgtg gtggatgatt tcattactaa taagaccatt aatataaaaa
     3181 aagaattata tcataaatat taacagaatg tatactgtat attatccatt caaatgattc
     3241 cgttatttat catacatcgg aatattgata ctaaagcact attcacatgc aaaaccaaca
     3301 tccgccatga aggactagct aaaacccaaa ctagtttgtt gcaattagca tccttgcacc
     3361 tctatgtaaa gggctgaagg gattcggatg tgatggtatg attacagaca ttcgtgtctg
     3421 agattgtctc tgactccata attcgaaggt tacagttatg atcatcgtta ccggcggcgc
     3481 gggctttatc ggcagcaaca tcgttaaggc cctgaatgat aaaggcatca ccgatattct
     3541 ggtggtggac aacctgaaag acggcaccaa gtttgtgaac ctggtggatc tggatatcgc
     3601 ggactatatg gataaggaag acttcctgat ccagattatg gctggcgaag agttcggtga
     3661 tgtcgaagcg attttccacg aaggcgcatg ctcttccacc accgagtggg atggcaagta
     3721 tatgatggat aacaactatc aatactccaa agagctgctg cactactgtc tggagcgcga
     3781 aatcccgttc ctgtatgcct cttccgcagc cacctacggc ggacgcacct ccgactttat
     3841 tgaatcccgc gagtacgaaa aaccgttgaa tgtctacggt tactcaaaat tcctgtttga
     3901 tgaatatgtt cgtcaaatcc tgccagaagc gaactcgcag attgttggct tccgctattt
     3961 caacgtttat ggaccgcgtg aaggccataa aggcagcatg gcgagcgtcg ctttccatct
     4021 caacacccag cttaataacg gtgaatcacc gaagctgttc gaaggtagcg agaacttcaa
     4081 acgtgacttc gtctatgtgg gcgacgtggc agatgtaaac ctgtggttcc tggaaaatgg
     4141 cgtttccggt atcttcaacc tcggcaccgg tcgtgcagaa tccttccagg cagtagcaga
     4201 tgctacgctg gcttatcaca agaaaggcca gatcgaatac attccgttcc cggataagct
     4261 gaaaggccgc taccaggcgt tcactcaggc agatctgaca aatctgcgcg cggcgggtta
     4321 cgacaaaccg ttcaaaaccg ttgctgaagg tgtaacggaa tacatggcct ggctgaatcg
     4381 cgacgcataa gagctctgca tgaaaatact ggtgatcggc ccgtcttggg ttggcgacat
     4441 gatgatgtcg caaagtctct atcgcacgct ccaggcgcgc tatccccagg cgataatcga
     4501 tgtgatggca ccggcatggt gccgtccatt attatcgcgg atgccggaag ttaacgaagc
     4561 tatccctatg cctctcggtc acggagcgct ggaaatcggc gaacgccgca aactgggtca
     4621 tagcctgcgt gaaaagcgct acgaccgcgc ctacgtctta ccaaactcct tcaaatctgc
     4681 attagtgcct ttcttcgcgg gtattcctca tcgcaccggc tggcgcggcg agatgcgcta
     4741 cggtttactc aacgatgtac gcgtgctcga taaagaagcc tggccgctaa tggtggaacg
     4801 ctatgtcgcg ctggcctatg acaaaggcat tatgcgtacc gcacaagatc tgccgcagcc
     4861 attgttatgg ccgcagttgc aggtgagcga aggtgaaaaa tcatatacct gtaatcaatt
     4921 ttcgctttca tcagaacgtc cgatgattgg cttttgcccg ggtgcggagt ttggtccggc
     4981 aaaacgctgg ccacactacc actatgcgga actggcaaag cagctgattg atgaaggtta
     5041 tcaggtggtt ctgtttggct ctgcgaaaga tcatgaagcg ggcaatgaga ttcttgccgc
     5101 tttgaatacc gagcagcagg catggtgtcg gaacctggcg ggggaaacac agcttgatca
     5161 agcggttatc ttgattgcag cctgtaaagc cattgtcact aacgattctg gcctgatgca
     5221 cgttgcggcg gcgctcaatc gtccgctggt tgccctgtat ggtccaagta gcccggactt
     5281 cacaccgccg ctatcccata aagcgcgcgt gatccgtctg attaccggct atcacaaagt
     5341 gcgtaaaggt gacgctgcgg agggttatca ccagagcttg atcgacatta ctccccagcg
     5401 cgtactggaa gaactcaacg cgctattgtt acaagaggaa gcctgacgga tgcgggtttt
     5461 gatcgttaaa acatcgtcga tgggcgatgt tctccatacg ttgcccgcac tcactgatgc
     5521 ccagcaggca atcccaggga ttaagtttga ctgggtggtg gaagaagggt tcgcacagat
     5581 tccttcctgg cacgctgccg ttgagcgagt tattcctgtg gcaatacgtc gctggcgtaa
     5641 agcctggttc tcggccccca taaaagctga acgcaaagcg tttcgtgaag cgctacaagc
     5701 agagaactat gacgcagtta tcgacgctca ggggctggta aaaagcgcgg cactggtgac
     5761 acgtctggcg catggcgtaa agcatggatt ggactggcaa accgctcgcg aacctttagc
     5821 cagcctgttt tacaattgta agcatcatat tgcaaaacag cagcacgccg tagaacgcac
     5881 ccgcgaactg tttgccaaaa gtttgggcta tagcaaaccg caaacccagg gcgattatac
     5941 tatcgcacag cattttctga cgaacctgcc tacagatgct ggcgaatatg ccgtatttct
     6001 tcatgcgacg acccgtgatg ataaacactg gccggaagaa cactggcgag aattgattgg
     6061 tttactggct gattcaggaa tacggattaa acttccgtgg ggcgcgccgc atgaggaaga
     6121 acgggcgaaa cgactggcgg aaggatttgc ttatgttgaa gtattgccga agatgagtct
     6181 ggaaggcgtt gcccgcgtgc tggccggggc taaatttgta gtgtcggtgg atacggggtt
     6241 aagccattta acggcggcac tggatagacc caatatcacg gtttatggac caaccgatcc
     6301 gggattaatt ggtgggtatg ggaagaatca agtagagtgt cgttctacaa gtatgtctct
     6361 tgcagatttg ccagctcaaa ccgtttttca aaatttaaat cttgaaataa taaccaataa
     6421 gttgacatcg gagataagat gacctcaaca ttatttttct ctctcgagaa aaaaaactgg
     6481 atagcgtact ggaacagagc tctcgtattc ttattcatta ccacctattt tttgggtggg
     6541 attacaaggt ataaacatct tattgttatt cttatgacaa taacgacaat cgtctatctc
     6601 tgcaaacggc caaaacacta tctctcacta tttaaaacat ttctttttgg tagtgttgcc
     6661 atattaacta ttgctgcatt gctgtcactt cttcaatccc ctgatgcagg tgctagcatg
     6721 aaggaagttt tcaaagctat tattgagaat actttactat gcacaatagc aataccggtc
     6781 atattgagag acgagaaaag agaagatgtc gaaaaaatcg ttttcttctc atttattagt
     6841 gcgttgggct tacgctgttt ttctgaattg attacctatt ataaggacta tcaacaaggg
     6901 ataatgccat tcgcagatta tagacaccgt agcatttctg actcgatggt ctttttattc
     6961 cctgcattgt taaatctctg gcttatcaaa tcagcaaaat accgcatttc ttttgtggtt
     7021 ctaagcgtta tttttatttt tctgatatta ggaactttat ccagaggggc ctggctttcc
     7081 gtgttagtca ttggattaat atggattctg atgtttaaac aatggaagtt actattagta
     7141 ggagtaatgg tttccatcat tgcattgtcg gttattttca cacataagga gatgaccgca
     7201 aagctaacgt ataaacttca acaaactaat agttcttatc gctatgcaaa tggtactcaa
     7261 ggcagcgcac tcgatctaat attagaaaat cctgttattg gttatggtta cggtaacgtt
     7321 gcatataaag atgtctataa taaacgtgtc attgattatc cagaatggac ctttagacaa
     7381 tcaatagggc cacataattt tgcgctattc atctggtttg gcactggttt attagggctg
     7441 gtaagtctta tgatgctata ctgtgcaata ttgaaagagt gtataaaaaa tggcgtcaag
     7501 aataaatatc gctcaccata taatgcatat tatataatct tactatcttt tataggttat
     7561 tttgttatcc gtggaaacgt agaacaaatt gaaccaaatt tattaggcgt ttacgccggc
     7621 ttattattag cgatgaaaaa caagtaaaaa ataaaaaagg ctgcataatg cggccttttt
     7681 atttattcaa accaattatg aataacctct tcgaatcttt gagttacgcc atcccaactg
     7741 tagtgatcaa acacaaagtc ctgaccttgc tttgcaactg ctgttaattc aggattcgct
     7801 aacgttttta aaatatcgct gcttatggag tctgcagtca taggttcttt taaatggaat
     7861 cccgtagtat tttctttgac aaattccgtc atccccccac gcgtactcac taaaacaggc
     7921 ttaccagcac ccatagcttc aattgccacc atgcaaaatg gttcctggaa ctgggaagga
     7981 atgacgacta aatctgccag tgggtaataa caatacatct tttcaggcgg tatgcttcct
     8041 aacataatgc aacgatcctt caaacgtttt gcaatttccc tgacattgcg ttgatatgca
     8101 cctttgtcac ttttactcat ttctgtataa tcaccaatca caactaattt taaatttttg
     8161 tgagcagcgg ccaatttttc gaatgattgc atcaacagca atatgccttt gtcagggacg
     8221 atacgcccag cataaaaaat aattttctct tcaggagaga tattaagttc gcttctagtg
     8281 atcggctgaa aattagattg ataggtttct aaatcaattc catttggtac aatctcaata
     8341 tctgcatttg ctaaataaga ttggtaatac tttttcaagt ataaactagg gactatcatt
     8401 ttaacatttt gctcaagcaa ttttggttca aatgcattat gcatatgtat aaccactctt
     8461 gcctgaggag ctagcttacg aatttgagtg tatagcttca tgctattatg aactacaata
     8521 acactttctt tcgttatagg aaaatcatgt gcaatattca atattctctg tgaatatgga
     8581 agaggatcaa gtcttgtcca tttttgaaat aaccgcttat atatgcgact gaatccaacc
     8641 ctatgaatac tacaattatc attaacaaag gtgtaatttg agtatcctgg atttttgata
     8701 caaacaatac gatttggaaa attggtacgc tgagcaacct gatacatcca cgtttcaaca
     8761 gcagccgctc cgcgaggagg aattgaaaaa ataggagtaa ccgtaaatat tattttatca
     8821 accatctcgt atattaacct ttcataacat tatatttaat tgctgtaaaa acaccatcga
     8881 gaaatttttt ctggtaaagc aagtgtttat atttttcttt gtgctcatgt tttttaacag
     8941 cttttttata aggtatattt ctccatggtg atatattata aatattacga aaataatctg
     9001 cagaggcgta gttcgcccaa tcatgccatg gcttagttat accagtataa tgtatgaaga
     9061 cagtgtcatc attaataaac cgggtgtaat attcactatt tttttcttca aattctgact
     9121 ttatagtata aatacaatta tattttctcg gtaatatttt agcatgatgt agaaacatta
     9181 agtttaaaat atcttgatcc gggtacttta atttttttat aattgactca tctgataata
     9241 gatcaaagaa tttttccgtt agtctttgtt ttaaccattc ccgtaaatta atatacatta
     9301 caccagagtt aaaatagcta ccgttaaaat cctcattaca caaacgctca gcgcttttac
     9361 tttgcatagc atctacatca attaccactg ccccatattc atctttaaac tctaatgcta
     9421 ttaactcgtt caatgaaccc ttacagacga tatcagcatc aagatataat aatgaatcca
     9481 atcgcgcaga aaaataatca aatgaaagca aacgataata aatagatact ggccagatat
     9541 ttgattgagg tagcgccttc aatggctcag aatcaatatg atataattta attactgttt
     9601 gatacttcgt tgctaattgt gataataatt caatatactc tttgtctaag taatcagcga
     9661 ttatgtgaaa ggtaaatttg ttttgtttat tcttttcaag aattgaagca attgaaatcg
     9721 cagccccaac ctggtagttc tcatcaatac cccatgagac attaaacgtt tcccgttcat
     9781 caagtttaac ggggcgcttg tccagttcaa ttatatcttt aaattgagta agatgtttaa
     9841 aatccaattt aattaccctt catcataatc attatttgct cttcaatcct agtttgactt
     9901 ttacatccct gattactttt ttaattttct ttttgaatat aagatatgta tagccaaagt
     9961 ctttcaattc attttttata ttatagtgcc gttcaagatc aatgcgatct tttgctttta
    10021 aaacagcggt tgttttttta cctgaaaggt caattagtct taaacctttt tcggatacta
    10081 taaaatttcc tttatgagga tcgccagaca ccattccatg ctgatgcaac tcttttatcg
    10141 attctgataa ttgatccttc agatcttcag aaatttccaa atactcattt aacccgacac
    10201 cttcaatata ctcaatgagc ataatataat aatgagcgaa atttaatgtt ttacgttcag
    10261 ctagcaaaaa atagtcatta attgattgga tgccttcacc acgtacccga tcggtttgat
    10321 aaattaaatt ttcataataa tcttttttta tgcaagattt taagaaacgt tcggtcattt
    10381 tatgctttgg cgcataaact ttaagtacaa gaggtccacg agcagtgtca ataagaatga
    10441 ctttagtatc atcaatagaa cgaaaaactt ttaatgtttt aaccctgcaa gttaaaaagt
    10501 catttagaac ttgctcataa aagggatcat tatcttttat gaaaactttc aacccattta
    10561 tcgttttatt atatatcata atgaattatt ttaaccttaa atctttagaa gcatttttct
    10621 ttataatact ttaaataatt aaaaatgcct gccatataat gcttttgttt aaatttatgt
    10681 tttgcacaat accgatactg attgctattt acaggtttaa gtaaatcttc tttactccac
    10741 ggagaagctg ctttggcgat caaaaaacta cgtgaaaccg gataatcagc ccactcatgc
    10801 cagggttttg taggtcccac atagtgtata aacacggtgt catcattgac tggattatca
    10861 actttgtctt ttaattcata gttaatacta tatcgggtat tatatttctc cgaaataaac
    10921 ttcactttac cattcaataa tacattcagt acatcttgat caaggtgggt gattttactt
    10981 acccaatctg ggtcacgcaa catttcaata gcttttgacg aaatgttatt tagattccac
    11041 tcatcaatat ttatcagtaa aaaaccagca ttgaaatatc cagaagctaa ctgtggtgta
    11101 gttaataccg aggctcgatt ttgccaccat tcaatatctc tttcagctac aactgcggca
    11161 atttcattag tagaaaattg ataatctaag agttctttaa tactaccctt gcaagcaata
    11221 tctgcatcaa gatatagtat tttttcatgt ttatgataga aataatctgc aattataaat
    11281 cgaaaatatg ttgcgtaagt ccagtttttc gtgcttggta atgacttcag cttatcacaa
    11341 ttgataacat aaatattaat tcttgaatta tattgttttg ctaaatcaga aaaatataat
    11401 ttgtctttat cactgatata atctgtgaaa acatgaaatt cacaagagat ttctctattg
    11461 tttaatagaa tagatgcgat ggctacacca catccaaaaa gaaaatttct gtcaatacca
    11521 aatgctatat caagagtatt tttagatctt atattttgat aatgaaaatt atactcaaaa
    11581 aggatgatgt cactatcatt gagttgagac atatttattc tgtatccaaa attttggcaa
    11641 aaataagtta cgggttacaa cgattttcga atcgttcttt ccctgatttt tgtggctttt
    11701 gcttctgctt gcgacagcag tccttgttcc tgcttgagaa tgtctttaag cggggcggca
    11761 aaatacacct tcataaagcg ccagatatcc cgctgagtca ggccaatatt cattgaagaa
    11821 aaataaagcc ctataagatc tttatcgcgc caacgacctg gaacgcgcgt gcgaagctgc
    11881 gcccggtgta ggtcaattac cgaaattttt aactcctctt ccttaccgga aaaaggcaag
    11941 tgcagcagga aatgacagat ataacagtca cgatggttaa tgcccgcagc atgcatatcg
    12001 cgcaccatcg tcgcgacacg cttaataagc atacgcttta cgcgaacatc tggtgggttg
    12061 gtcgcccagt cagcacagta atcttccaga ctgatggttg gtgtcagatc ttcggtaata
    12121 ataaacgagg ttctggtcag cggattcatg cctttttcgc cgaatgccac cccatacata
    12181 gtatcaacgc cgacatcccg cagtcgatga atcgcattcc attcgcggtc tgcgcctaat
    12241 actggcatcc gcaatgagag taaatttttg attatctctt tcagggtcgt gccgcgatgc
    12301 catttgagaa aatagctttt tcccgccatt tcaaagcgca gagtacggcg agtttccagt
    12361 tcacgaaata cctcaccctg caaggtttta acttcctcaa aaggatcttt gccgcgccat
    12421 aacgtggcaa acggctcttt aagttcaacc atccagacca cccgttatga tatccgccgc
    12481 tttctctggc agactgtata aatcttgtgt atcagcataa tgtcgcgcat tttccgccca
    12541 agcctggcgc aatgaagatt gcgttaacgc tttgcgtaaa atctcattca atgtttcctg
    12601 gcggaatggc tcagcaatag cctcgccgca attagcgtcg acaatataat gcgcatagcc
    12661 acaaacggca gttgttagta ccggtaatcc tgcagtaatc gcttccagca gcacaattcc
    12721 cgccgcttcc tggtaggcag gatgcagtaa taaatccgcc gccgccatta attccgagac
    12781 atcgttgcgc cccgagaaga agtgaacatt actgcgcacg ccgcgttttt ctgccagtgc
    12841 ctcaaatttt cgcggtttat cctgcccaac aacatatagc aatgtgttgt ggcgcagcga
    12901 atccggtaac gaagcaagtg cttcaatgga acgatcggca cctttacgcg tgaagtctga
    12961 accgacctgc aacaataaat attgttgttc ggttattcca ttcttcttac ggaagatttc
    13021 acggctattg gctggctgct ggctatattt acgatcagga taaatccccg gtggcagaat
    13081 atgaaaacgc tccgcttcag tctgataatg tttctggaaa tcggcgattt gcttatctgt
    13141 cagcatcagc agctgtgtcg gcttgccctg ttcgaaggtt gcccgctcaa aggcggcata
    13201 atggcgataa cgtgacgtca ggcgatagaa aaagcctttt tcctgcgcta ctttctcggc
    13261 ataacaaaca tcagcggcat aataaacgtc cagccccggc attttgttga atccaacgac
    13321 tttatcgacg ggatgttcgc gtaaatgttt ttgcacccag gcaaaatact ccgcattgcg
    13381 cccgtgattg gtatgcgatt taaccggcac tttgatcact tcaaatacat cagggcattc
    13441 gccttcccac gactgggtat aaacccgaac atgatgacct cgggcggcga ctgtctgagc
    13501 aatacgcata aaatcgcgct gcaaaccgcc aaagggaaaa tatttatata aacaaaaagc
    13561 aacgatcata acgacgtacc tgtcgtggag gagggcagta atttatcgac agcagcaatg
    13621 acatctgccg ccggaataac cgaaagatac atctcatttc ggtcacgctg atcgcgcgtt
    13681 ggcatttccc ggtaatctcc cgcccagaat tgaatcatgt tatttgacca gggacgccag
    13741 aaaatatgat ctgttgcgcc aaacagcgat atcagcggcg tattaactgc agcggcaata
    13801 tgcgccggtg cggaatcaac gccaataaac agctgcgcat gatcgattaa cgcaccaagt
    13861 tccgggaagg tcacctttcc agccagcgcc gttactggtg gcgtctggca tccctgcgca
    13921 atttcattga cgcaggccag atcgtcttta tctgggccgg acgtcagaac gacttcataa
    13981 ccacgagcat gtaaggcatc aatcacagcg gaaaacttgg cgttgtccca gcatttgaag
    14041 atttgccgcg ccgtaggttg gataaccaca taattttgtc caacaccagc gtgatcaagt
    14101 tcgcgacgca tacgtttcca gcttgcaggc gggtaactca tggttgtctg cttcaccaac
    14161 gaatcaagtc ccaatggtgt cagcacggat aagttacttt ccaccacatt tccaccctgc
    14221 aacggcacca aatgggtgaa acttttacgc caaaaagcag actgccgatg atgataatcc
    14281 tgggaaattt tcacacgagc atttaataag cgaaccagta tagcaaccat ccattgatcg
    14341 gtaagattga cgataaggtc atacttattg gcacgtaata ccttgatgag atgaaaaaag
    14401 ttggcaattt tttctgaggc ttttgctttt ttatttttta tgccgtagag cgcgttaatc
    14461 tctggatttt cagacaggat cgggatggtg tcctgataaa gcagcacatc gatttttgcg
    14521 tcagggtaat tttttttcag agaactaatg acgggagtag ttaataacat atccccatga
    14581 aaacgcatct taatgagcaa aattcttcga aatggcttat ccacaagtaa ctctttttgt
    14641 tgagattatc cgattaaggt gaagcagaaa aagcacgcta ccgccccagg ctcaacagct
    14701 acctgaatac tgataccgca ctgatattga cgcgctagtg tatcacttct ttcgcggcaa
    14761 tccgacccga atgattttta agcacattgt tgattttcaa taaagaaatc aatcaaataa
    14821 aatcattagc aaccatattc tttcactgtt gctctgcgat tccttcctgc acgtacagca
    14881 gaaatcaatg aatcgcataa aaatacaaaa aaagtataga tgttcagcaa gaattggcgg
    14941 gtaaattgct atttaagtca aa
//
LOCUS       DAJVPP010000092        14835 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVPP010000092 DAJVPP010000000
VERSION     DAJVPP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.092"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..492)
                     /locus_tag="OQM34_003043"
     CDS             complement(<1..492)
                     /locus_tag="OQM34_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1453398.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRM"
     gene            766..1233
                     /gene="slyB"
                     /locus_tag="OQM34_003044"
     CDS             766..1233
                     /gene="slyB"
                     /locus_tag="OQM34_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1453399.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(1280..1714)
                     /gene="slyA"
                     /locus_tag="OQM34_003045"
     CDS             complement(1280..1714)
                     /gene="slyA"
                     /locus_tag="OQM34_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1453400.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            1915..2151
                     /locus_tag="OQM34_003046"
     CDS             1915..2151
                     /locus_tag="OQM34_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1453401.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2154..3011
                     /locus_tag="OQM34_003047"
     CDS             2154..3011
                     /locus_tag="OQM34_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1453402.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3011..5023
                     /locus_tag="OQM34_003048"
                     /pseudo
     CDS             3011..5023
                     /locus_tag="OQM34_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5024..5545)
                     /gene="sodC"
                     /locus_tag="OQM34_003049"
     CDS             complement(5024..5545)
                     /gene="sodC"
                     /locus_tag="OQM34_003049"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS1453403.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(5626..6522)
                     /locus_tag="OQM34_003050"
     CDS             complement(5626..6522)
                     /locus_tag="OQM34_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1453404.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(6571..6810)
                     /locus_tag="OQM34_003051"
     CDS             complement(6571..6810)
                     /locus_tag="OQM34_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1453405.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            6913..7512
                     /gene="nemR"
                     /locus_tag="OQM34_003052"
     CDS             6913..7512
                     /gene="nemR"
                     /locus_tag="OQM34_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1453406.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7549..8647
                     /gene="nemA"
                     /locus_tag="OQM34_003053"
                     /pseudo
     CDS             7549..8647
                     /gene="nemA"
                     /locus_tag="OQM34_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            8728..9135
                     /gene="gloA"
                     /locus_tag="OQM34_003054"
     CDS             8728..9135
                     /gene="gloA"
                     /locus_tag="OQM34_003054"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1453407.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            9238..9885
                     /gene="rnt"
                     /locus_tag="OQM34_003055"
     CDS             9238..9885
                     /gene="rnt"
                     /locus_tag="OQM34_003055"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1453408.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            9978..14436
                     /locus_tag="OQM34_003056"
                     /pseudo
     CDS             9978..14436
                     /locus_tag="OQM34_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(14480..>14835)
                     /locus_tag="OQM34_003057"
     CDS             complement(14480..>14835)
                     /locus_tag="OQM34_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1453409.1"
                     /translation="GRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAY
                     TSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSG
                     RSLKQEPTEMIQATA"
BASE COUNT         3370 a         3927 c         3908 g         3630 t
ORIGIN      
        1 cattcgtcgc tcggttgggt gagccagcaa ggcatgatgg aacgcaggta ccagcggtgc
       61 gccttgtcct cccaaggcaa tatcgcgacg gcgaaaatca ccgacaaccg taattccggt
      121 gcgtgccaca atttgattgt tatcgccaat ctgcaaagtg tgtggtgcca cgcccgtcgg
      181 ttcatgccag acggtttgac cgtgacagcc gatcgcaact atatctctcg cctgcaggtt
      241 ttgttcctta agcaaggcat tgaccgcatc agcaaaaagt cgcccgagtt gagtatcaag
      301 ctgtccaaac tgcgaaagtg taagctgctg gccctggcaa atatccagta cagcctgttt
      361 cagagacacc gggatcggcc agctcaagct tgccagctgt gcgacccggt gttcatcaat
      421 tgtcgccaac acaacatcaa caccatcaag gctggtgcct gacataacgc caataaagcg
      481 gcccgatttc atagttcatc ctttttcaat ctgacgtttg cgcaccactc aaacataaac
      541 ttttcgtgaa taccatgcgg aatgaccgat ttttaccgtt ggtagtaaaa cattatcttc
      601 aaatcaataa tcatcatgaa tgttttgttt ataattggtt gatcctactt tcattatgat
      661 ttgctcatat ttggtagaac atgtaaccat ggattcacat atgccatata ctttgaccat
      721 gagggatgct tgcgtggcgt ttcatggtga acaggagatt tttcaatgat taaacgcgta
      781 ttggttgttt caatggtagg tctgtctctt gtcggttgtg ttaataacga caccctgtca
      841 ggggatgttt ataccgcttc tgaagcgaaa caagtacaga atgtcagcta tggcaccatc
      901 gttaacgtac gtccggtaca gattcagggc ggtgatgatt ccaacgttat cggtgcaatt
      961 ggcggtgctg ttcttggtgg tttcctgggg aatactgttg gtggcggaac cgggcgttct
     1021 ctggctactg cagcaggcgc tgttgcaggt ggcgtagctg gtcagggcgt acagagtgca
     1081 atgaacaaaa cgcagggtgt cgagctggaa attcgtaaag acgatggtaa taccatcatg
     1141 gtggtacaga aacaaggcaa cactcgtttc tctccgggcc aacgtgtcgt actggccagc
     1201 aatggcagtc aggtgaccgt ttctccgcgc taaataagtt tgcgtgtggt caggttactg
     1261 accacacgcc cccttcattt caccctttgg cctgtaactc aatgatatta tgctcaagtt
     1321 ttgcgatgag cgtaatcagt tgctccagtt cctctgcgga gatgccatgt aatatttccg
     1381 cgcgggtttt gttaataaca gcttccattt cgctgatcag tggctctgcc ttttccgtca
     1441 gtttaatacg tttagcccga cgatcgctgg cacaagtttg acgcgaaatt aacccttttt
     1501 cttccagttg gtccagagta cggaccagtg atggctgctc gatgccaatc gcttttgcca
     1561 gttgaatttg cgactggtct ggaggtaact gatggatatt gtgtaacgta acccaatggg
     1621 tttgtgttaa ctccagcggt ttcaggcgat ggtctatcag agcacgccat atgcgcacca
     1681 accgtgccag atcagaacct agtggcgatt ccaatttcat ctccttataa ttagcttgct
     1741 aagatattat gcggctttta gaatagtgtg cagcaattgt attgctaaaa caaatgtatt
     1801 gctgcatttg gttaccgtca gacatatttt tcagaaattg cgcgtaaatt tttcgcactt
     1861 aaagaatatt tattaatcta acgcaatata ttcggtcgta aaggaatcta ctttgtgaag
     1921 tttatgctca atgcaacagg attgccctta caagatctgg tgttcggtgc gtccgtctat
     1981 tttcctccgt ttttcaaagc attcgcgttt ggattcgtca tctggcttgt cgtacaccgc
     2041 ctgcttcgtg gctggatcta cgccggcgat atctggcatc ccttgttaat ggatttatcg
     2101 ctgtttgcga tttgcgtttg ccttgctctg gcaatactga ttgcgtggta actatgtcaa
     2161 ttaaaacaat taagtatttc tcaacaatca ttgtagcgat agttgcggtt cttgccggat
     2221 ggtggctgtg gaattattac atgcaatctc cgtggacacg cgacgggaaa atacgcgcag
     2281 aacaggtttc tatcacccca caggtgtcgg gacgtattgt tgagctgaat ataaaagaca
     2341 accaactggt taacgcgggg gaacttctgc tcacgatcga taaaacgccc tttcagatcg
     2401 ccgagctgaa cgcacaggct cagctggcga aagcgcaatc tgacctcgca aaagccaata
     2461 acgaagcgaa tcgtcgtcgt catctctcac aaaattttat ttttgccgaa gaactcgata
     2521 ccgctaacct caatgttaaa gcgatgcagg ccagtgttaa tgccgcacag gcgacgctga
     2581 aacagacaca atggcaactg gcgcaaacgg aaattcgcgc cccggtgagt ggatgggtga
     2641 ctaacctcac tacccgcatc ggcgattacg ctgacaccgg gaaaccgctg tttgcccttg
     2701 tcgacagcca ctcgttttac gtcattggtt attttgaaga aaccaagttg cgccatatcc
     2761 gcgaaggtgc accagcacaa attaccctct atagtgataa caaaacgtta cagggtcacg
     2821 tttccagtat cggtcgggcg atttatgatc agagcgttga aagtgactca agccttattc
     2881 cggatgtgaa acctaacgtc ccctgggttc gtctcgccca actcgttccc gtccgctttg
     2941 cgctggataa agttccaggc gatgtcacgc tggtgtcggg caccacctgt agcattgccg
     3001 taggtcaata atgaacgcat cgtcatggtc cttgcgcaat ttgccctggt tcagggccac
     3061 gctggcgcaa tggcgttatg cgttacgcaa taccattgcc atgtgtctgg cgctgacggt
     3121 tgcctattat ttgaatctgg atgaacccta ttgggcgatg acctcggctg cagtggttag
     3181 ctttcccacc gttggcggtg tcatcagcaa aagcctcgga cgcatggctg gcagtttact
     3241 gggagccatt gcggcactgc ttcttgccgg acatacgctc aatgagccgt ggttttttct
     3301 attgagcatg tcggcgtggc ttggcttttg tacctaggcc tgtgcgcact tcacgaataa
     3361 cgtcgcgtat gcatttcaac tggcgggcta cacggctgcc atcatcgcct ttccgatggt
     3421 taatattact gaggccagcc agctgtggga tatcgctcag gcgcgcgttt gcgaggtgat
     3481 agtcggtatt ttgtgcggcg gcatgatgat gatgatcctg cctagcagtt ccgatgctac
     3541 tgccctttta actgcattga aaaacatgca cgcccgatta ctggaacatg ccagtttact
     3601 ctggcagcct gaaacaaacg atgccattcg tgcagcacat gagggggtga ttgggcagat
     3661 actgaccatg aatttgctgc gtatccaggc tttctggagc cactatcgtt ttcgccagca
     3721 aaacgcgcgc cttaatgcgc tactccacca gcaattacgt atgaccagtg tcatctccag
     3781 cctgcgacgt atgttgctca actggccctc accgccaggt gccacgcgag aaattctcga
     3841 acaattgctg acggcgctcg ccagttcgca aacagatgct tacaccgtcg cacgtattat
     3901 cgccccgcta cgcccgacca acgtcgccga ctaccggcac gtcgccttct ggcagcgact
     3961 acgttatttt tgccgccttt atctgcaaag tagtcaggaa ttacatcgtc tgcaaagcgg
     4021 tgtagatgat catgccagac tcgcacggac atccgctctg gctcgtcata ccgacaacgc
     4081 cgaagctatg tggagcgggc tgcgtacatt ttgtacgttg atgatgattg gcgcatggag
     4141 tattgcatcg caatgggatg ccggtgccaa tgcattaacg ctggcagcaa ttagctgcgt
     4201 actctactcc gccgtcgcag caccgtttaa gtcgttgtca ctgctgatgc gcacgctggt
     4261 gttactttcg ctattcagct ttgtggtcaa atttggcctg atggtccaga ttagcgatct
     4321 gtggcaattt ttactgtttc tctttccact gctggcgaca atgcagcttc ttaaattgca
     4381 gatgcccaaa tttgccgcac tgtgggggca actgattgtt tttatgggtt cgtttatcgc
     4441 tgtcactaat cccccggtgt atgattttgc tgattttctt aactataatc tggcaaaaat
     4501 cgttggcgtt gcgctggctt ggctagcgtt cgccattctg cgtccaggat cggatgctcg
     4561 taaaagccgc cgccatattc gcgcgctgcg ccgggatttt gtcgatcagc taagccgcca
     4621 tccaacactg agtgaaagcg aatttgaatc gctcacttat catcacgtca gtcagttgag
     4681 taacagccag gatgcgctgg ctcgccgttg gttattacgc tggggtgtag tgctgctgaa
     4741 ctgttctcat gttgtctggc aattgcgcga ctgggaatcg cgttccgatc cgttatcgcg
     4801 agtacgggat aactgtattt cactgttgcg gggagtgatg agtgagcgtg gcgttcagca
     4861 aaaatcactg gcggccacac ttgaagaatt acagcggatt tgcgacagcc ttgcccgtca
     4921 tcatcaacct gccgcccgtg agctggcggc aattgtctgg cggctgtact gctcgctttc
     4981 gcaacttgag caagcaccac cgcaaggtac gctggcctcg taattactta attacaccac
     5041 aggcatagcg ttcaccgcca ccgcccagcg gtttaggttg atcggacata ttatcgccgc
     5101 caacgtggac catcagcgcc ttgtctttga tttcatccag tgatttcaga cgaggcgcga
     5161 tgacggcatc ggtagctttg ccgtcattat tgacgaccag tgcaggcaga tcgcctaaat
     5221 gcccggtacc ttctggccct tcatgtttac cggtattttg tggatcaaga tgcccgcctg
     5281 cggattccgc ggcgctggct ttgccatctt tggtggctgg ctggcagctt cctttggcat
     5341 gaatatggaa gccatgttca ccggggggta atgctttcag atcgggtgaa aactccagac
     5401 ctttatcggt ttcagtaatg gtgacgctac caattgactg cccaacccct tgcgacgtga
     5461 cgaggttcat ctcgactttt tcactggcag cttgtgcgcc ggtcgcaaca accagcgcca
     5521 gaatggccag actaaaacgt ttcataggac ctccgttcat tgtgcattcc tgaataagtg
     5581 taaaccagtg acacattttt gaacggcggc tattcctaaa agtgcttacg gtacgtcgta
     5641 ccccagtgca gctttacgga tacgaaacca ttgttgacgg gtcattttca gtgtttctgc
     5701 ttcgacagct gcccgtacgc gctcaatttt acctgagccg ataattggca gcggctgcga
     5761 cggtaaacgt aatacccagg cgtaaaccac ctgttcaatc gagcccgcgt ttaactcctc
     5821 cgccaccaca gccagttcat cacgcagcgg ctggaaataa tcatcattaa acagacgacc
     5881 accaccaagg caggaccacg ccatcggacg aacacgcagt tgttgtagtt ggtcgagcgt
     5941 gccatccagc agtaacggct gatgcaccgg ggatatttcc acctgattag tggcaagggt
     6001 aaacggcaga cgtgattgca acagggcaaa ttgcgcaggc gtaaagttcg atacgccaaa
     6061 atgacgcact ttgccgctct gatgcagatg tttgaacgcg tccgccactt catcggcatc
     6121 cattaacggg tctggtcggt ggattaacag cagatccaga tgatcggtcg cgagattaat
     6181 tagcgactgt tcggcgctct taatgatgtg attgcggtca gtgatgtaat gaccaatgac
     6241 gttttcttca cgcgcggtcg tcgcgatacc gcatttactg acgatttcca tccgttcacg
     6301 caggtgaggt gccagtttca gtgcctcgcc aaacgccgct tcgcactgat agccaccata
     6361 aatatcagca tggtccacgg tggtcacgcc gagatccaga tgctcttcaa taaaactgac
     6421 cagctggcgg gcggacatat tccagtccat caatcgccag tagcccatca caaaacggga
     6481 aaactccggg ccttccggcg caatagtaat acgctgaacc ataatcgctt cctcttatca
     6541 gatataagag gagtatacgc aagattaggt tcaaaagagt gatggttgct ccggttcgtc
     6601 tgatgacgct ggcttatttg cgcgtaattt gcgcattaat cgctgccgac aaaggcgcag
     6661 cacctcttgt ttttcgccat cgctcatttt attccagtta aaacgctcat cccgactacg
     6721 aaaacagccg cgacaaaacc cgcgttcgtc agactggcaa attccccggc acgggctctg
     6781 gacgggaaag aactctaatt gctccgccac ctcgccctcc tcagataaga ttattaccat
     6841 tattgaagct gttaatgtcc aaagtagcaa ctttgcttgc actagaccga ctggtctact
     6901 acactccaac gcatgaacaa acacaccgaa catgatactc gcgaacatct cttggcgacg
     6961 ggcgagcaac tttgcctgca acgtggattc accgggatgg ggctaagcga attactaaaa
     7021 accgctgaag tgccgaaagg gtccttctat cactactttc gctctaaaga agcgtttggc
     7081 gttgccatgc ttgagcgtca ttacgccgca tatcaccagc gactggctga gttgctgcaa
     7141 tccggcgaag gtaactaccg cgaccgcata ctggcttatt accagcaaac actgaaccag
     7201 ttttgccaac atggaaccat cagtggttgc ctgacagtaa aactctctgc cgaagtgtgc
     7261 gatctgtcag aagatatgcg cagcgcgatg gataaaggtg ctcgcggcgt gatcgccctg
     7321 ctctcgcagg ctctggaaaa tggccgtgag aaccattgtt taaccttttg tggcgaaccg
     7381 ctacaacagg cacaagtgct ttacgcacta tggcttggcg cgaatctgca ggccaaaatt
     7441 tcgcgcagtt tcgagccact ggaaaacgcg ctggcccatg taaaaaacat tattgcgacg
     7501 cctgccgttt agcaggcatt ttttatcacc agacgaccgg gagcctttat gtcatctgaa
     7561 aaactgtatt ccccactgaa agtgggcgcg atcacggcgg caaaccgtat ttttatggca
     7621 ccgctgacgc gtctgcgcag tattgaaccg ggtgacattc ctaccccgtt gatggcggaa
     7681 tactatcgcc aacgtgccag tgccggtttg attattagtg aagccacgca aatttctgcc
     7741 caggcaaaag gatatgcagg tgcgcctggc atccatagtc cggagcaaat tgccgcatgg
     7801 aaaaaaatca ccgctggcgt tcatgctgaa aatggtcata tggccttgca gctgtggcac
     7861 accggacgca tttctcacgc cagcctgcaa cctggcggtc aggcaccggg tagcgccttc
     7921 agcacttagc gcgggaacac gtacttctct gcgcgatgaa aatggtcagg cgatccgcgt
     7981 tgaaacatcc atgccgcgtg cgcttgaact ggaagagatt ccgggtatcg tcaatgattt
     8041 ccgtcaggcc attgctaacg cgcgtgaagc cgcttttgat ctggtagagc tccactctgc
     8101 tcacggttat ttgctgcatc agttcctttc cccttcttca aaccatcgta ccgatcagta
     8161 cggcggcagc gtggaaaatc gcgcacgttt ggtactggaa gtggtcgatg ccgggattga
     8221 agaatggggt gccgatcgca ttggcattcg cgtttcgcca atcggtactt tccagaacac
     8281 agataacggc ccgaatgaag aagccgatgc actgtatctg attgaacaac tgggtaaacg
     8341 cggcattgct tatctgcata tgtcagaacc agattgggcg gggggtgaac cgtatactga
     8401 tgcgttccgc gaaaaagtac gcgcccgttt ccacggtccg attatcggcg caggtgcata
     8461 cacagtagaa aaagctgaaa cgctgatcgg caaagggtta attgatgcgg tggcatttgg
     8521 tcgtgactgg attgcgaacc cggatctggt cgcccgcttg cagctcaaag ctgagcttaa
     8581 cccacagcgt gccgaaagtt tctacggtgg cggcgcggaa ggctataccg attacccgac
     8641 gttgtaatcc aacattgcga gcggcgtaaa gccgccgcta tactaaaaca acattttgaa
     8701 tctgttagcc attttgagga taaaaagatg cgtcttcttc ataccatgct gcgcgttggc
     8761 gatttgcaac gctccatcga tttttatacc aaagtgctgg gcatgaaact gctgcgtacc
     8821 agcgaaaacc cggaatacaa atactcactg gcgtttgttg gctacggccc ggaaaccgaa
     8881 gaagcggtga ttgaactgac ctacaactgg ggcgtggata aatacgaact cggcactgct
     8941 tatggtcaca tcgcgcttag cgtagataac gccgctgaag cgtgcgaaaa aatccgtcaa
     9001 aacgggggta acgtgacccg tgaagcgggt ccggtaaaag gcggtactac ggttatcgcg
     9061 tttgtggaag atccggacgg ttacaaaatt gagttaatcg aagagaaaga cgccggtcgc
     9121 ggtctgggca actaatctcc tgccgggcgt gaactcatcg cgcccgcatc tttactgcat
     9181 cgacaagtaa tatttgtcat aatgcgcgct gcaatttatc cgtattaaga gaatcagatg
     9241 tccgataacg ctcaacttac cggtctgtgc gaccgttttc gtggttttta tcctgttgtg
     9301 atcgatgttg aaacagccgg atttaacgcc aaaaccgatg cgctgcttga gattgccgcc
     9361 atcaccctga aaatggatga acaaggctgg ctgatgccgg acaccacatt acatttccac
     9421 gtcgaaccat ttgtcggcgc aaatttgcaa ccagaagccc tcgccttcaa cggcattgac
     9481 ccgaacgatc ccgatcgcgg cgcggtcagc gaatacgagg cgctgcacga aatttttaaa
     9541 gttgtacgta aaggtattaa agcgagcggc tgtaaccgcg ccattatggt ggcgcacaac
     9601 gccaattttg atcacagctt tatgatggcc gccgcagaac gcgcctcgct gaaacgtaac
     9661 ccgttccacc ctttcgccac ttttgacact gctgcactgg ccgggctggc actcggacaa
     9721 accgtattgt caaaggcttg ccagaccgct ggcatggact tcgacagcac ccaggcgcac
     9781 tccgcgctgt acgacaccga acgcactgct gtgctgtttt gtgaaatcgt caaccgctgg
     9841 aaacgtctgg gaggctggcc gctacctgcc gccgaagagg tgtaatcgag tcgatgcctg
     9901 atgacatgca atgattcagg catctatagt gaggctattc cacgcatcaa gcatgatatt
     9961 cacggggaat agcgttaatg gcagataatc cagacccttc atcgctcctg ccggacgtgt
    10021 tttcaccggc gacccgcgac tggtttcttc gcgcctttaa acagccgacc gctgtccagt
    10081 cgcaaacctg gcatgtggcg gcgcgaggcg aacatgcgct ggtgattgca ccgactggct
    10141 ccgggaaaac gctggcagca tttctctacg ccctcgatcg gctcttccgc gaaggcggcg
    10201 aagatacccg cgaggcgcat aagcgtaaaa cctcacgcat cctctatatt tcaccgataa
    10261 aagccctggg caccgacgtt cagcgcaact tgcagatccc gttgaagggt attgccgatg
    10321 aacggcggcg gcgcggcgaa acggtaatta ttgatgaagt ccacgcggta gcgggcagta
    10381 aacgtggtgc gcatctggcg ttaagtctgg agcggctcga tgcgctgctc cacacctcag
    10441 cacagcgaat tggcctttct gccactgtgc gctcagccag cgatgtggca gcatttcttg
    10501 gtggcgatcg cccggttacg gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa
    10561 ttgtcgtacc ggtcgccaat atggatgatg tctcatcggt cgccagcggc accggcgaag
    10621 acagccatgc cggccgggaa ggctccatct ggccatatat tgaaacgggt atccttgatg
    10681 aagtgttgcg ccatcgctcg accattgtct ttactaattc acgcgggctg gcggaaaaac
    10741 tgacagcacg attaaatgag ctttacgccg cacgcttaca gcgttccccg tctatcgccg
    10801 ttgatgcggc ccatttcgag tcgacctccg gcgcaacctc taaccgtgta caaagtagcg
    10861 acgtttttat tgcccgctca caccacggct ccgtctctaa agaacaacga gcaatcaccg
    10921 aacaggcgct gaaatcgggt gaattacgct gcgtggtcgc aacctccagt cttgaactgg
    10981 ggattgatat gggcgcggtg gatctggtga ttcaggtggc aacgccgctt tctgttgcca
    11041 gtgggttaca acgcattggt cgcgccggac atcaggttgg cggtgtatct aaagggctgt
    11101 ttttcccccg tacccggcgt gatttagtcg attccgcagt cattgtagag tgtatgttcg
    11161 caggcaggct ggaaaacctg acaccaccgc ataatcctct cgacgtcctt gcgcagcaaa
    11221 ccgttgccgc cgcggcgatg gatgcattac aggtagacga atggtactcc cgcgtacgcc
    11281 gtaccgcacc gtggaaagat ctgccaagac gtgtttttga cgccacgctg gatatgcttt
    11341 ccgggcgcta tccctctggc gatttttctg cttttcgccc caaactggtc tggaacaggg
    11401 agaccgggat attgaccgcc cgacctggcg ctcaattgtt ggcggttacc agcggcggca
    11461 ccattccgga tcgtggcatg tatagcgtgt tattacccga aggtgaagaa aaggccggtt
    11521 cgcggcgggt gggtgaactg gatgaggaga tggtatatga gtcgcgggtg aacgacatta
    11581 tcactctcgg cgctacctca tggcggatcc agcaaatcac ccgcgatcag gtgattgtga
    11641 ctcctgctcc gggtcgttct gcccagctcc ccttctggcg tggtgaaggt aacggacgtc
    11701 cggctgaatt aggcgagatg atcggcgatt ttcttcattt actggcggat ggcgcgttct
    11761 tttccgggac tattcccccg tggctggcag aagaaaatac gaacgccaat attcaggggt
    11821 tgattgacga gcaacgcaac gcgacgggca tcgttccggg gagtcgccat ctggtcctcg
    11881 aacggtgccg tgatgaaatt ggcgactggc gtattatttt gcactctccc tatggaagac
    11941 gggtgcatga accctgggcg ctggcgattg ccgggcgaat acatgcccta tggggcgctg
    12001 acgcgtctgt ggtcgccagt gatgacggca ttgttgcgcg cattcctgac actgatggta
    12061 aattgcccga cgccgcgatt tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg
    12121 aggcggtagg cagctcggca cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat
    12181 tattaatgcc gggccgcacg ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg
    12241 ccagtcagtt gctggaaatc gctcagggat atccggattt tccggtcatt ctcgaaaccc
    12301 tacgcgaatg tctgcaagat gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc
    12361 tgaacggtgg cgaaattcaa atatccgatg taacgaccac tacgccctcg cctttcgcca
    12421 caagtttatt gttcggctat gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag
    12481 agcgccgggc atccgtactg tcgctggaca gcgagttact gcgcaatcta ctcggacagg
    12541 tcgatccggg ggaattactc gacccgcagg tcattcgcca ggtggaagaa gagttgcaac
    12601 gactggctcc tggtagaaga gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac
    12661 tggggccaat gaccgttgaa gacctggcgc aacggcatac aggcagcagt gaagaggttg
    12721 cgtcgtatct ggaaaatctt cttgcagtaa aacgaatctt cccagcgatg attagcggac
    12781 aggagcgtct tgcctgtatg gatgatgccg ccaggctgcg tgatgccctc ggcgtacggc
    12841 taccagagtc attgccagag atttatttac atagagtcag ttacccgctt cgcgacctct
    12901 ttctgcgcta tctccgggct catgctctgg tcacggctga acaactggct catgagttta
    12961 gtctcggtat tgccattgtc gaagagcagc ttcagcaact gcgtgaacag ggtctggtga
    13021 tgaatctgca acaagacatc tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct
    13081 cgctgcaagc cgccagagaa gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc
    13141 tgctggaacg tcagggcgta ttacccgcca ccgatggtag cccggcgctc tttgcctcaa
    13201 catctccagg cgtttatgag ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg
    13261 gagtcggttt acccgcctca ctctgggaaa gccagatcct gcctgcccgc gtacgcgact
    13321 attcgccaga aatgctcgat gaattactgg caaccggagc ggttatctgg tcgggacaaa
    13381 aaaaagctgg gtgaagatga cggcctggtg gcactgcatc tacagaaata tgccgcagaa
    13441 tcgttcactc ccgccgaagc ggatcaggcg aatcgttcgg cgctgcagca agcgataatc
    13501 gctgttctgg ctgacggagg agcctggttt gcacaacaaa tcagccaacg gatacgcgat
    13561 aaaatcgtcg aatcggttga ttcctcggca ctgcaagagg cgttatgggc gctggtctgg
    13621 caaggcgtca ttaccagcga tatttgggca ccgttacgcg ccctcacccg cagcagttcc
    13681 aacgcacgca cctcaactcg ccgcagtcac cgggctcgtc gtgggcgtcc tgtctatgca
    13741 caacccgtct cgccgcgggt atcttacaac acaccaaatc tggctggacg ctggtcgtta
    13801 ttgcaggtgg agccactaaa cgataccgaa aggatgctgg cgctggcgga aaatatgctc
    13861 gaccgctacg gcatcatcag tcgtcaggcg gtgatagccg aaaatatccc tggcgggttt
    13921 ccatcgatgc aaacactttg tcgaagtatg gaagactccg ggcgaattat gcgaggtcgt
    13981 tttgtagaag gtctgggtgg cgcgcaattc gctgaacgtc tgactattga ccgattgcgc
    14041 gatctggcga cacaagccac gcaaacacgc cactatacac cagtggcgct ctccgccaac
    14101 gatccggcta atgtgtgggg aaatcttctg ccctggcctg cacatccggc aacgctggta
    14161 ccaacgcgtc gggcgggtgc gctggtggtc gtttctggcg gcaaattgtt actctatctg
    14221 gcgcaaggcg gcaaaaaaat gctggtctgg caggaaaagg aggaattact cgccccagag
    14281 attttccagg cgctgaccac cgcactgcgt cgcgaaccaa ggctgcgctt tacgctaaca
    14341 gaagtgaatg atcttccggt ccggcaaacg ccgatgttta cgctgctgcg tgaggcggga
    14401 ttttcaagtt cgccacaagg gctggattgg ggatagttga catcctccac gccctgaaag
    14461 acgtggttcc ctgctacgtt caagctgtcg cctgaatcat ttcggtgggt tcctgcttca
    14521 acgagcggcc tgactgcacc atctctccac aggcaagaac ggcgtgcccc gccgctaaaa
    14581 tgttacgagc gccatttaca tcggcgttcg ctgtatatcc acatacctgg catctgaatt
    14641 tactttgtga caggcggttt tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg
    14701 tgtacgctgg cggaacagca agcacctgac cgccactcca gagctgcttg tactcaagct
    14761 ggcggcgcat ttcataccag ccctgatcca gtatcgaacg gtttaaacct gattttgccc
    14821 ggacattgcg ccccg
//
LOCUS       DAJVPP010000093        14633 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVPP010000093 DAJVPP010000000
VERSION     DAJVPP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.093"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(121..546)
                     /gene="arsC"
                     /locus_tag="OQM34_003058"
     CDS             complement(121..546)
                     /gene="arsC"
                     /locus_tag="OQM34_003058"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS1453410.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(559..1848)
                     /gene="arsB"
                     /locus_tag="OQM34_003059"
     CDS             complement(559..1848)
                     /gene="arsB"
                     /locus_tag="OQM34_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS1453411.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1902..2255)
                     /gene="arsR"
                     /locus_tag="OQM34_003060"
     CDS             complement(1902..2255)
                     /gene="arsR"
                     /locus_tag="OQM34_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1453412.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2998..3081
                     /locus_tag="OQM34_003061"
     CDS             2998..3081
                     /locus_tag="OQM34_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1453413.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3135..4487)
                     /gene="gorA"
                     /locus_tag="OQM34_003062"
     CDS             complement(3135..4487)
                     /gene="gorA"
                     /locus_tag="OQM34_003062"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1453414.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4559..5401)
                     /gene="rlmJ"
                     /locus_tag="OQM34_003063"
     CDS             complement(4559..5401)
                     /gene="rlmJ"
                     /locus_tag="OQM34_003063"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1453415.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWQLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5604..7646
                     /gene="prlC"
                     /locus_tag="OQM34_003064"
     CDS             5604..7646
                     /gene="prlC"
                     /locus_tag="OQM34_003064"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1453416.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7654..8406
                     /gene="rsmJ"
                     /locus_tag="OQM34_003065"
     CDS             7654..8406
                     /gene="rsmJ"
                     /locus_tag="OQM34_003065"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1453417.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8455..9924)
                     /gene="dtpB"
                     /locus_tag="OQM34_003066"
                     /pseudo
     CDS             complement(8455..9924)
                     /gene="dtpB"
                     /locus_tag="OQM34_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10242..10676)
                     /gene="uspA"
                     /locus_tag="OQM34_003067"
     CDS             complement(10242..10676)
                     /gene="uspA"
                     /locus_tag="OQM34_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1453418.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11070..11405
                     /gene="uspB"
                     /locus_tag="OQM34_003068"
     CDS             11070..11405
                     /gene="uspB"
                     /locus_tag="OQM34_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1453419.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11476..12975)
                     /gene="pitA"
                     /locus_tag="OQM34_003069"
     CDS             complement(11476..12975)
                     /gene="pitA"
                     /locus_tag="OQM34_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1453420.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGERIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13207..14406
                     /locus_tag="OQM34_003070"
     CDS             13207..14406
                     /locus_tag="OQM34_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS1453421.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3645 a         3749 c         3875 g         3364 t
ORIGIN      
        1 agtaagttgg cagcattacc tgaatttgca cggttttgta ggtcggataa gacgcgttag
       61 cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga tatcgccgct
      121 ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaatgc
      181 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
      241 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      301 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
      361 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
      421 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
      481 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      541 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      601 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
      661 agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc gttgccaggc taccaatcgg
      721 ggtaattttc ggccccaaat cgcagccaat cacgttggca taaaccatcg cttctttgat
      781 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
      841 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
      901 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
      961 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggggcacc
     1021 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
     1081 tgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
     1141 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
     1201 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     1261 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1321 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     1381 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     1441 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     1501 aagcgccgcg ccatcattgg caaacagggc cgcaacggcg gcaccgagca ggacaatcca
     1561 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa acgtgcagcg ctgcccattc
     1621 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcaataa acgccgccgt
     1681 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatgaacca cgcccgtaac
     1741 caacgccagt accgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     1801 gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
     1861 ataaaacaca tgtgcaaatc catatgtgtt tagctaaatt tttaactgca aatgttctta
     1921 ctgtctccgg aacagttttg tctggccagg ttgcggacaa ttgcctgaat cttttcctgt
     1981 tcacatcgcc aggcttcatc aataattttc gccgcccatg ctggaatatg cggcgataag
     2041 cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     2101 gccagatggc gggatatctt gggctgcgac tgatcgaggg cagtgcagag atcgcagacg
     2161 cataactctc ccagttcgct aagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2221 attttgaaca attggatggg taacagaaat gacatattgc gctcctgatt gttgcaggta
     2281 gtgtctctct tcgaagcgga taagtcaaaa acatatatga cttaacgaat gtgtaagtgc
     2341 agaggaaggt aataggtgtg aattttgagt tggctattca tttgaaaggc ggtttgaatc
     2401 aggacgtaaa aaaaccgact ttgcgtcggt tttttactct tcagcctgag ttggtggctc
     2461 tggctggagt gagaatgccc acatctaacg gttcaatact attagccagt ggctaacatt
     2521 gcaagcgtta gcgatttctt cagctggaga agggacagtt tgctatacat ggtggtgaaa
     2581 gaggatttat atactttcat cgggtcgctg ccgtaccaag ccttgtccga atccggacgt
     2641 aaaaaaaccg acttcgcgtc ggttttttta ctcttcagcc cagagttggt ggctctggct
     2701 ggagtgagaa tgccatcatc taacgcatca atgctaatac catactgaaa ctattgcaag
     2761 gttatgctgg ttatttagcc taattacact gtatgattat ccagttagct ctgaggcatt
     2821 ttcactctgg caatgcgcat aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc
     2881 gttaactgat gctctggccg gagtgagatt gcccacatct aacaatgagg catgcgccgt
     2941 gacaggcagt ggatgagtaa gcggatgcat tctcactcca tcgcatggag aaaacgggtg
     3001 attgataaag caatcatcgt tcttggggcg ttaattgcgc tgctggaact gatccgcttt
     3061 ctgcttcagc ttctgaactg atagcggaaa cgtaattaag ggctaagagc acactactct
     3121 tagcccttta acatttaacg catggtcacg aactcttctg ccgccgttgg gtgaatggcg
     3181 acggtattgt cgaagtcttt tttggttgcg cccatcttca gcgccaccgc gaagccctgc
     3241 aacatttcgt ccataccaaa gccaatgccg tgaataccga caatcttctc ttctggccca
     3301 acgcacacca gcttcatgcg gcacggctgg cggtgagtgg tgacggcggt atacatcgcg
     3361 gtgaaagagg atttatacac tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt
     3421 tccgttaaac caacagtacc aatcggcgga tggctgaaga ccacggtcgg aatgttgctg
     3481 taatccagat gctcatccgg cttgttatta aacaggcgtt cagagagacg gcgacccgct
     3541 gcaactgcca ccggtgtcag ttccactgca cccgtgttat cgcccaccgc gtaaatacct
     3601 tcaatattgg tgttttgata tttatcgacg acgatatagc ctttttcgtt ggttttaacg
     3661 ccagcggctt cgaggttgat gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg
     3721 caatccaccg tttcactgcg accatcttcc agctccagcg tcaggctacc atcggcattt
     3781 ttcactaccg ctttcgggat ggcgttggta tgcaactgtg ggccttcggc gttcatcact
     3841 tcgaccagcg tttcagagat catcgggtcg aagctgcgca gcggcgcatg tttacgcaca
     3901 aacagatgcg ttttcgcgcc gaggccgtta atcacgccag ccagctcaac ggcgatgtaa
     3961 cccgcgccaa caaccgccac gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa
     4021 tcaataccgt attccacgcc tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc
     4081 agaatatgat cggccgtgat ggtttcgccg ttaacttcca gcgttttggc atcaacgaag
     4141 cgggcaaagc ctttgattac atcaacgtta tttttaccga gcacgttttc ataggaagta
     4201 tgaatacggt cgatataggc ggtacggctg gcgatcaacg tttcccagtt gaacttgttg
     4261 atggtggtat cgaaaccata atcagggccg tacatatgga tcgcttcacg gatttgcgcc
     4321 gcgtgccaca tcactttttt cggcacgcag ccgacgttca cgcaggtgcc gcccagctct
     4381 ttggcttcaa tcagtgcgca tttctggccg tacatagccg cgcggttgat ggaggcgata
     4441 ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct
     4501 tatcgttgat taccgcgatt gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt
     4561 actccggcac gatccagctt acggtggcgt gcccggtacc tgccggaacc agtttgctgt
     4621 gcagccacga cagcacgtta ttcatctgct gttccagctg ccacggcggg ttaatcacaa
     4681 tcatgccgga agcggtcatg ccacggcgat cgctgtctgg cagcaccgcc agttcaattt
     4741 gcagaatttt gcgaatgccg gtcgcttcca ggtcgtggat catgcgctta atttgctggc
     4801 gcagcaccac cggataccac aatgcgtaag tgccagtggc gaaacgtttg taaccttctg
     4861 ctatcccgct gaccaccgcc tgatagtcgg ttttcatttc atacggcggg tcgataagga
     4921 ttaaaccacg gcgagaaacc ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt
     4981 tttcgacgcg tgcacggctg tctttctgaa atccagaacg cagcaacggg taatcgctcg
     5041 ggtgcagttc ggtcagttgc aggctgtctt gttcgcgcag tagctggcga gcaatcaacg
     5101 gcgaaccggg gtagtaacgc aactggccgc tacggttgaa gtgttttacc acattgatgt
     5161 acgcctccag ttctgcgggc aaatcgtcct gctgccagat acgggcgata ccttcgagat
     5221 attcgccggt acgctcggca tgttcgctac ctaactgata acgcccggcc cctgcgtggg
     5281 tgtcgagata gagaaacggt ttatctttct ctttcagcga ctcgatgatc aggctctgaa
     5341 cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa gctgtggcga taactgagca
     5401 tgggtaaagg tgttccggta agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat
     5461 caggacagta taccgaaaag aggccgccgc cgcgaaagcg taacgtttct cattgaaatt
     5521 cactacactt aaccccatgc tacacacatt atgtaaagcg cctgttgagc gcttccttaa
     5581 cctctttaac caggactgcg ctaatgacga atccgttact gactcccttt gaattgcctc
     5641 cgttttctaa aattctcccg gaacatgtcg ttccagccgt gactaaggca ttgaacgact
     5701 gccgcgaaaa tgtggagcgc gtagtagcgc aaggggcacc gtacacctgg gaaaatctct
     5761 gccagccgtt ggcggaagtg gacgatgttt tggggcgtat cttctccccg gtcagccacc
     5821 tgaactcggt gaaaaatagc ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc
     5881 tgtcggaata cagcacctgg gtagggcaac atgaagggct gtataaagcg taccgcgacc
     5941 tgcgcgatgg cgatcattac gccacgctga acacggcgca gaagaaagcg gttgataacg
     6001 ccctgcgtga ttttgaactc tctggcatag gtctgccgaa agagaaacag cagcgttacg
     6061 gcgaaattgc tacccgtctt tctgaactgg gcaaccagta cagcaacaac gtcctcgatg
     6121 cgacgatggg ctggaccaaa ctcgttaccg acgaagcggg gctggcgggg atgccagaaa
     6181 gcgcgctggc tgcggcaaaa gcccaggccg aagcgaaaga gctggaaggc tacctgctga
     6241 cgctggatat cccaagctac ctgccggtaa tgacctactg cgacaaccag gctctgcgcg
     6301 aagagatgta tcgcgcttac agcacccgcg cctccgatca aggcccgaat gccggtaagt
     6361 gggataacag caaggtgatg gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc
     6421 tgggctttga aaactacgcc tttaaatccc ttgccactaa aatggcagaa aacccgcagc
     6481 aggtgctgga tttcttaacc gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc
     6541 tggagcaact gcgtgccttc gctaaagccg aatttggtgt cgatgagttg cagccgtggg
     6601 atatcgctta ctacagcgaa aaacagaaac agcacctcta cagcatcagc gacgagcagc
     6661 tgcgtccgta cttcccggaa aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta
     6721 tttacggcat caccgctaaa gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt
     6781 tcttcgaact gtatgacgaa aataacgaac tgcgcggcag cttctacctc gatctgtatg
     6841 cccgtgaaaa caagcgcggc ggggcgtgga tggatggctg cgtaggccag atgcgtaaag
     6901 ctgatggttc tctgcaaaaa ccggtcgcgt atttgacttg taacttcaac cgcccggtaa
     6961 atggtaaacc ggcgctgttc actcacgacg aagtgatcac cctgttccac gagttcggtc
     7021 acggcctgca ccatatgctg acccgcatcg aaaccgctgg tgtttccggt atcagcggtg
     7081 tgccgtggga tgcggtcgaa ctgccgagtc agtttatgga aaactggtgc tgggagccgg
     7141 aggcgctggc gtttatctct ggtcactatg aaaccggcga accgctgccg aaagagttgc
     7201 tggataaaat gctggcggcg aagaactacc aggcggcgct gtttattctg cgtcagctgg
     7261 agttcggcct gtttgatttc cgccttcatg ccgagttccg cccggatcag ggggcaaaaa
     7321 tcctcgaaac tctggcagaa atcaagaaac tggttgccgt ggtgccatct ccgtcctggg
     7381 gccgtttccc gcacgctttc agccatattt tcgccggtgg ttatgcggca ggttactaca
     7441 gctacctgtg ggctgacgta ctggcggcag atgctttctc gcgctttgag gaagagggca
     7501 ttttcaaccg tgaaaccggg cagtcgttcc tcgacaacat tctgagccgt ggcggttcag
     7561 aagagccgat ggatctgttc aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc
     7621 tggagcatta cggcattaag ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca
     7681 ggcgccggag acggcgcctt atctgttctg gcggcccgct gggggctgga gcacgatgaa
     7741 gacaacctga tggcgctggt gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag
     7801 ccaaaacttg gcggcatctt tgttgatttt gttggcggag cgatggcgca ccgacgcaaa
     7861 ttcggcggtg gtcgcggtga ggcggtggcg aaagcggtgg gcattaaagg cgattatttg
     7921 ccggatgtgg tggacgccac cgcagggctg gggcgcgatg cctttgtact ggcttcagtc
     7981 ggctgccgcg tgcggatgct ggagcgtaat ccagtggttg ccgcgctact cgacgacggc
     8041 ctggcgcgtg gttatgcgga tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta
     8101 attcacgcct ccagcctgac ggcgctaacc gatattaccc cacgcccgca ggtggtctat
     8161 ctcgatccga tgttcccgca taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc
     8221 tttcagtcgc tggtggggcc ggatcttgat gccgatggat tactggagcc tgcacgcttg
     8281 ctggcgacca aacgcgtggt ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc
     8341 gccacgccaa acgcggttgt gactaaaggg catcgctttg atatctatgc aggtacgcca
     8401 gtgtaaaagt aaagcagggt agcgataacg ctaccctgct ttctgcaaga ataattaatg
     8461 gctttccggc gtcgcaatca tacgtttcag ccacggcacc atcaacagca tcactactgc
     8521 aacgcccagc gtgaccagac caatcttacc aaacacgttg gtatagacgg gcaacgtctc
     8581 aagcggatcg gtaatgttgt ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa
     8641 gaacgcggca gcctgcgtca ggaaccacat cccgagaata aagcccatca aatgctgcgg
     8701 caccagggca gcaatcatcg ccaggccaag ggcgctaata aacaattcac ccaggctcta
     8761 gaataaatac accagcacga taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca
     8821 cattcccgca gcagcagccg tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa
     8881 tttcatcggc atcgagaggt ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag
     8941 tattgggctg gcgagtacca cccagaacgg gttaagcgcc tggaagctga ccgggttgat
     9001 ggaaaaaccg agaatttcat gatgcacgtt gttgatggca aagaagttca gcgatgttgg
     9061 catctgggcg tagagaatgt aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa
     9121 cattttattg cgcccggttt tatccagctt gaatgcctga cgaaagaaga tgatagtgac
     9181 gacgatggag agaacaatca gcaccagatt ggcgacttct acgttgtgca tcagccatgc
     9241 acatacgaag atcatcacca cgctgccaag taatacgtac aacagtttgc tgaagctcat
     9301 cggcttgaag tcgggttcag aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac
     9361 cagtaatgcg ataattaacc ccgccccgca caggttgtag gtgactgaat aaccgaatct
     9421 atcagcgatc acaggggcca gcgataacgc tatcaacgag ccgatattga tcgacatata
     9481 gaacagggtg aatgcgccat caagccgcgg atctttcggc ggatagcact tcgaaagcaa
     9541 gctggctggg ttagctttaa acaggccgtt accgacagcg atagtcccca gggcgatgaa
     9601 aatcaggtca ggcttaagta gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag
     9661 tgctccaaga acaatggtgc gtttggtccc caacaggtgg tcgccgacat agccgccaat
     9721 ggaaatgagg ccatagacca gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg
     9781 cgagaatcca agctgtttaa cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc
     9841 gaatcgctcc cataactcga caaaaaagat catgaaaaat gggcgaggtt gctgcagcat
     9901 ccccatgggt gttgttgtat taatatttat taaccttcca ataccatcct gaaaagtatg
     9961 aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc agagattctt aatattgatg
    10021 cggtgaatag attaatacac tattacaaca gaaaataacc agctgatcac actaatgatg
    10081 gaaaatatga tggtgttaaa ttttgtattg gcaggagatt ttgattgcgt acgcagatcg
    10141 taggccagat aaggggttta cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc
    10201 ccgcatatgc gggcgttcag gaacacgtcg tggagggatg attattcttc ttcgtcgcgt
    10261 agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc
    10321 agtttgctcc agaagtcctg gtggtgacca caaaccacca gatccatatc gtatttcttg
    10381 attgcatcga ccagaacctg gcctaggtcg ccgctgccgc tcagggtttc agtgattgga
    10441 tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc
    10501 tgcatatcac ccagattcac atcaataagc ccggtgtata ggtcagagta gtttacatct
    10561 acgtggatca gagaaacttt cgcattgtag gggcgagcca tagagactgc tttctctacc
    10621 agaactttgc tttccgggga gagatcgact gcgatgagaa tgtgtttata agccatagtg
    10681 ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg ccgttacgtc
    10741 acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag
    10801 accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt acccgttaga
    10861 taaacaaatt ttcgacatga atattatatt gatagtggtt aaccttctgg aaaaaaaaca
    10921 acctgatctc ctacactatc tatagagccg ctcggatgtt ggtcaccaca aaagcggttt
    10981 ctccaggacc tggtgttaac tgattggcag cgtatcggga gcggtagccc cggggtggaa
    11041 ctccgcgggc aggtcgccgg ggaggaggta tgataagcac cgtcgcatta ttttgggctt
    11101 tatgtgtcgt ttgcattgtt aacatggcgc gctatttctc atcactacgc gcgttgttag
    11161 tggtactgcg taactgcgat ccattgctct atcaatatgt tgatggaggg ggctttttta
    11221 cctcacatgg ccaacccaac aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt
    11281 atcgcgatca tcacgatgat gagtttattc gccgctgtga gcgggtgcgt cggcagttta
    11341 ttctgactag cgcattgtgt ggtctggtgg tggtcagcct gattgcattg atgatttggc
    11401 attaagcact tcagcaataa taacgtataa aaaaagcggg ccagtaaact gacccgctcg
    11461 ttttggtgcg tacgattaca ggaactgcaa ggagaaccag tacagcccgc cggaaagtag
    11521 taccgcagcc ggaagggtaa acacccaggc catcagaata ctggttacgg ttttacgctg
    11581 taagccgccg ccatctacca ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt
    11641 ggaaaccggc atcccggtat aactcgccag gccgattgac actgccgccg tcatctgggc
    11701 agacatcccc tgagcgtagg tcatgccttt cttaccgatt ctctcaccga tagtcgttgc
    11761 cacacgacgc cagccaatca tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat
    11821 ccacaccggc gcatactcga tggtgctaag catgtcggac ttcagtttct tcaacaggcg
    11881 ctgatcgtca gcactcacac caggcatctt caccactttg tcgatagtgt cagaaacgca
    11941 cagcataatg cggcgcatct ggctacgttg atcaagcgac agcttgtcgt agctttccac
    12001 atcggtggtc aacatacctt tcaggcggtt gagcgcgtta atggtattcg acggatggca
    12061 gtggaactcc gcaggttgcg ttgcgccagc ttccggagcc ggtactaact gatcagcacc
    12121 ggtagcctgt ttgagcagcg caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc
    12181 atcacgggta cgggtgattt cgtagccagt ggcattcatg ttcaccacga agcctgctgg
    12241 cgcgacgcca atcaatacca acataaccag accaatgcct ttctgaccat cgttcgcgcc
    12301 gtgcgaaaac gccacgccga tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg
    12361 ctttttcttg ccgtctttct tttcacgctc cgctggggtc aggtggatac gggcgcgttt
    12421 cttggtgccg ctccagtagc gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc
    12481 gacaataggg gaaacgatca gagaaccgaa aatacttaat actttcggga tattgagtgc
    12541 atccaccact gacgtcccgg tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc
    12601 aatcagcgta tgagagctgg atgcaggtaa accaaagtac caggtaccca ggttccagat
    12661 aatcgccgcc agcaacatag agaacaccat ggcaaggcca tgagacgatc ccatattaag
    12721 cagcagatcc gtcggcagca tatgcacaat ggcataggca acactcagac cacccagcaa
    12781 aacacccaaa aagttgaaca ccgccgccat aaccacggcg agctgagaac gcatcgcgcg
    12841 ggtatagata acggttgcca cggcgttggc tgtgtcatgg aaaccattga tggcttcgta
    12901 gaacagcaca aaagccagtg caagcaataa taacagcccg gtatgcaaat ccaggccagc
    12961 aaacaaatgt agcataggac gttacgccat tttgaggaca tgaacgcggc gcattatcag
    13021 tgactttcgc ggcgcgggca aagtgaaata tagacttttt ttgatttgcc tcctgtatgg
    13081 atttcactca aaaaataatt atcttatata attcaggcaa atacttcctt ttagtaatat
    13141 tgatgctggt gcgaccactg aggaatcttt acaattcacg cccgtttttt ctaagaggag
    13201 cgcaacgtgg aaaggtttga tgccattatt ataggcgctg gtgcggcggg tatgttctgt
    13261 tctgcgctgg caggtcaggt aggacgccgg gttctgctga tcgataatgg taaaatacta
    13321 gggcgcaaaa tccttatgtc tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa
    13381 ccaggcgctt atctgagcca gaatccgcat ttttgtaagt ctgcactcgc acgttttacc
    13441 cagtgggatt tcattgatct ggtcaataaa cacggtatcg cctggcacga gaaaacgtta
    13501 gggcaactct tctgcgatga ctccgcgcag cagattgtcg acatgctggt ggatgagtgc
    13561 gagaagggca atgtaacctt cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa
    13621 acaggcttca cgcttgatct gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg
    13681 accggtgggc tgtcaatgcc ggggctgggc gcatcgccgt ttggttataa gattgccgaa
    13741 caatttggcc tcaacgtgct gccgacccgc gcgggtctgg tgccattcac tctgcataaa
    13801 ccgttgctcg aagagttaca ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct
    13861 gaaaacggca ccgttttccg tgagaactta ctcttcaccc accgcggctt atctggaccg
    13921 gcggtgttgc agatttcaag ctactggcaa ccgggtgaat ttgtcagtat caatctgcta
    13981 ccggatgtgg acctcgaaac cttcctgaat gagcagcgta acgcacatcc gaatcaaagc
    14041 ctgaaaaaca cactggcggt tcatctaccg aagcggttgg ttgaacgctt acagcaactc
    14101 gggcaaatcc cggatgtttc gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt
    14161 agcacattga ccgactggcg cgtacaaccc aacggcactg aaggctatcg cactgccgaa
    14221 gtgacgctcg gcggcgtgga caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa
    14281 gtgcctgggc tgtacttcat cggcgaagtg atggacgtta ccggctggct ggggggctat
    14341 aacttccagt gggcgtggtc gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg
    14401 aaataaccat gacgttttcc cgtcagccgt ttgtatatgt cgttattaag tcattttggt
    14461 acggttctgc atccgacatt ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat
    14521 tgccgttgtg gttattttat agtgtattta ttctcagaga atatatatta ctgaaataaa
    14581 aaacacaaag aataaccaaa ggaaattatt tgaaacgtaa aaggtatata ttt
//
LOCUS       DAJVPP010000094        14399 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVPP010000094 DAJVPP010000000
VERSION     DAJVPP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.094"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(237..599)
                     /locus_tag="OQM34_003071"
     CDS             complement(237..599)
                     /locus_tag="OQM34_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCS1453422.1"
                     /translation="MRDIQMVLERWGGWAASDNSGVDYSPIAAGFKGLLPQTGKNRLS
                     CTDADGLIIEGCLARLKKKKPYEHSLLVAHYLYGLSKRKIAKEKKKDEKLIRIEIKMA
                     EGFIEGCLSMLDIRLEMD"
     gene            complement(617..1606)
                     /locus_tag="OQM34_003072"
     CDS             complement(617..1606)
                     /locus_tag="OQM34_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1453423.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPDSFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALATGVLA"
     gene            complement(1614..2429)
                     /locus_tag="OQM34_003073"
     CDS             complement(1614..2429)
                     /locus_tag="OQM34_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCS1453424.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYS
                     RTSINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(2592..2987)
                     /locus_tag="OQM34_003074"
                     /pseudo
     CDS             complement(2592..2987)
                     /locus_tag="OQM34_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            complement(2984..3310)
                     /locus_tag="OQM34_003075"
     CDS             complement(2984..3310)
                     /locus_tag="OQM34_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCS1453425.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTVVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(3310..3804)
                     /locus_tag="OQM34_003076"
     CDS             complement(3310..3804)
                     /locus_tag="OQM34_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1453426.1"
                     /translation="MIMSLLNEVQKYIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEAEPQPVRETRPVRNFYVGTNDPREILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSSDEWYLSGN
                     YVGA"
     gene            complement(3801..4742)
                     /locus_tag="OQM34_003077"
     CDS             complement(3801..4742)
                     /locus_tag="OQM34_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1453427.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(4732..4911)
                     /locus_tag="OQM34_003078"
     CDS             complement(4732..4911)
                     /locus_tag="OQM34_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1453428.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(5087..5638)
                     /locus_tag="OQM34_003079"
     CDS             complement(5087..5638)
                     /locus_tag="OQM34_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453429.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(5682..5882)
                     /locus_tag="OQM34_003080"
     CDS             complement(5682..5882)
                     /locus_tag="OQM34_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1453430.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            5973..6647
                     /locus_tag="OQM34_003081"
     CDS             5973..6647
                     /locus_tag="OQM34_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HCS1453431.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            6882..7088
                     /locus_tag="OQM34_003082"
     CDS             6882..7088
                     /locus_tag="OQM34_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453432.1"
                     /translation="MHKTEPKIVAPGYTNEEIYEWMAKKLAAINQLREVLSYRQETID
                     SLKKLDQEITVLSQDVTLDIVQTN"
     gene            complement(7060..7494)
                     /locus_tag="OQM34_003083"
     CDS             complement(7060..7494)
                     /locus_tag="OQM34_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453433.1"
                     /translation="MSGNIYTLYKSHCENVGKYRGIEISGVVSSVEISKVESRATLLT
                     LLDLVLHEHRKKFGTPYNQLNGKKALVHLILMKHHWMPKQINEMKFDELLLSIQDELT
                     LDKISVTAQKFLDYRDWRSQIHHFDDFDENEWDPNLSAQYLK"
     gene            7638..7948
                     /locus_tag="OQM34_003084"
                     /pseudo
     CDS             7638..7948
                     /locus_tag="OQM34_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7945..8325
                     /locus_tag="OQM34_003085"
     CDS             7945..8325
                     /locus_tag="OQM34_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453434.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGR"
     gene            8391..9215
                     /locus_tag="OQM34_003086"
     CDS             8391..9215
                     /locus_tag="OQM34_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HCS1453435.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            9343..9879
                     /gene="yfdR"
                     /locus_tag="OQM34_003087"
     CDS             9343..9879
                     /gene="yfdR"
                     /locus_tag="OQM34_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1453436.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            9870..10232
                     /locus_tag="OQM34_003088"
     CDS             9870..10232
                     /locus_tag="OQM34_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453437.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            10232..10537
                     /locus_tag="OQM34_003089"
     CDS             10232..10537
                     /locus_tag="OQM34_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453438.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            10764..11927
                     /locus_tag="OQM34_003090"
     CDS             10764..11927
                     /locus_tag="OQM34_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1453439.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(11942..12017)
                     /locus_tag="OQM34_003091"
     tRNA            complement(11942..12017)
                     /locus_tag="OQM34_003091"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11982..11984),aa:Thr,seq:cgt)
     gene            complement(12132..13385)
                     /gene="proA"
                     /locus_tag="OQM34_003092"
     CDS             complement(12132..13385)
                     /gene="proA"
                     /locus_tag="OQM34_003092"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1453440.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(13397..>14399)
                     /gene="proB"
                     /locus_tag="OQM34_003093"
     CDS             complement(13397..>14399)
                     /gene="proB"
                     /locus_tag="OQM34_003093"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1453441.1"
                     /translation="CAQLHAAGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAV
                     GQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNNIVPVINEN
                     DAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPRRNPQAELIKDVYGI
                     DDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGVIGDVMEGISVG
                     TLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFSRG
                     EVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         3728 a         3660 c         3414 g         3597 t
ORIGIN      
        1 gcaattggta tgttcgctat ttccagagag tcgatagttt atctatattt tatatctgct
       61 tgtggaggag gcattgctgc tgcaatgtat tcttttttct ttatcagctc aataaacagc
      121 gaaatacaga gatttaaaag catcttatta cacagagctg aagcgaataa aaaaacgaaa
      181 gaatttatag actcattaaa caaaaaggcg gattgaaatc cgcctttttg ttttcttcaa
      241 tccatttcta atctaatatc caacattgaa agacaacctt cgataaatcc ttcagccatt
      301 ttaatctcaa tgcgtataag tttctcgtcc tttttctttt ctttagcaat ttttctctta
      361 gaaagaccat acaaataatg tgctactagt aacgaatgct cataaggttt tttcttcttc
      421 agccgagcaa ggcacccctc aatgattaat ccgtcggcat cagtacatga gagtctgttt
      481 ttacctgttt gtggtaaaag ccctttaaat cctgctgcga ttggagaata gtcaacgcct
      541 gaattatcac tagcagccca tcccccccaa cgctcaagaa ccatctgaat atcacgcatc
      601 aaatttctcc acaaaatcag gccagcacgc cagttgccag cgcacgatcg ataaaacgaa
      661 atatcagctc cagctgagag ccatacttct cttcaaatgc cacggtatcc gcatgcagct
      721 cgtcgtgatg ctttctgcac aaaggcaaca caaagaggtc atgcgctttt gtacccattc
      781 ccccctgacc gtggcctatc aggtggtggg gatcatcagc aggttttcca caacatgcgc
      841 acggctgcgt cttaacccaa cgcgtgtact tttcgttaac ccagcggcga cgttttgggc
      901 gtaacataaa agagtccggc gactccggat ccactttcag cgccagcacc ttttttgcct
      961 tatcctggat gatgctggtg gcaggaaccg aaggcacaag gtcactttcc cgggtgacag
     1021 acggcacaac aggctttggt aatctcagtg ccttacgggc tgcactttcc ggtaaggcat
     1081 ccgccaggtc attacgaacc agccaccagc acagttccgg cattgtcaca acgtgactat
     1141 catcaaaacc gagatcccga cgcacaacag acaacaccca gcgggcacag ttatccgttg
     1201 ccattgattc cagccgttcc gtgaactgat cgcgcagctg gttatcgcag tgccagcaca
     1261 gacggattgc gcccggagcg tgtcgcattg tggtcatgtt ctcgctgtgc cagtcggaat
     1321 gaggccactg gcagcctttt tcacgaagta accagctttc aagacattcc acgccaccag
     1381 cacgacggat cactgcctca ttgcggaaca cggcccgaac ggcaggatca tccgccagcg
     1441 gttgtgatgc cgccggaacg gcaccgctgg cgaaagatga ataacgttcc ggctcaggct
     1501 ccagcaggac acgcccctgc ataaacaggg gcatcagctc tgaaccaggt ctgaacaata
     1561 cgatccccat acgcggggca atttcagggg tcagtagtgc tctcacagtc acctcaatga
     1621 acggtatcga gcagctttaa cagctcaggg aatcgggatt cgaagaaatg cggctgcgtc
     1681 tcgcgcggat ttgcgggact ggtgatgttc ttgccgaaca tgcagccttt cgctgtcagc
     1741 gaccagaatt ttttgatgtt gttaatcgag gtacggctgt atcgttcgcg ctgctcgacg
     1801 atccccagct tcaccatctg gtgatatgcc tgattagccg tcaggcggat accatactgc
     1861 ttcagcagtg cactcagcga cagcgtaggg cggcttgaac catctggcgc atcagcaggt
     1921 gcatcaatgg catagatcgg cataagttca ggaagaccag ctacctttga taatttctgg
     1981 tatgcaccaa gtttcgagga gtttgacaga tttagagtct ttgctgctga ttcaagcaga
     2041 atgaccccgg atttaatttt gtcggatgtg gtttcttctg gtgatgaatt atgaagcgca
     2101 tcaaaagtac gtatcacttt taagctgaat gccgggctga tccacattgc atatgcatag
     2161 accagctctt tacagacata cgtcccacca ttgcgcccct gaatggtgat gacaggaata
     2221 ctacgggaat ctcccgtagt ttcttcttcc aataattcca caagagcctt cgtttcagga
     2281 cgacgcataa actcgtgaac ttccagcgaa cgggaggagc gattctcacc agcggcaaga
     2341 agagcagctt tctgaaggtc gttaagacag tagttagatt cgaagtactg gcgcacagaa
     2401 acgccatcaa ttacaagcaa ctgattcatt ggtttctcca caaattttta tccacgagcg
     2461 ggactgcact cccttttcgt tgatgcagga tgaacttact gcgattttta atagttatca
     2521 aggatatact gttcataaat acagtatctt taacgaggta atacccaaat ttagggtgtt
     2581 gctcaattcc gttaccgagt tgctaatttg caactcgctt tttcgtactt actgatagtg
     2641 atctcgacct tcccctccgg gataaccggt ccccactcca ccagcattct tttcacctga
     2701 ctgtcgtctt cccacacacc cgcgtgggtc agggcgtcaa acagcgcctt gttatagttg
     2761 tccagatcgc ggatccggtt atccggagga aacaaaacga tctccactga agcaggtgcc
     2821 gacgttggtt tcggcagacg acgtaactgc tcaactattg ctgcgcacgc cgcgctctgg
     2881 aattttcacc ccgccgcgct tatcaggctc ttaccagcaa acgccccttt gttggggtgt
     2941 cgccagtacg tgttcacgct gggcggaaaa ggaaggatca gcttcatact ttcaggcccc
     3001 tctcatgtaa ccagtgggct gcacgcagcc tggcgttttc ctcaccggca agcagtgagc
     3061 ggataatccc gaccgcctcg ctgtcgtcgt ccttcaccac ggtatgaagc gtgatccccc
     3121 gggccacacc acgctttatc gtgatgacgc cttttttctc cagtgcgcga agatgctcca
     3181 ccgctgcatt caccgaacgg tatcccagca tggttgccac ctcctgattg gttggcggga
     3241 agccacgttc tttctgataa gaaatcagca tatccagcac ctgctgctgg cattgagtta
     3301 acgtcgtcat tacgccccca cgtaattccc tgacagatac cactcatcac tcgatacagc
     3361 gcgcttgctg cttttccgta aacactgctc acgacgcgcc agaaaattgt ttcgttctgg
     3421 ctgggagtgg ctttcacgga atgccgccat ccacaccgtt gcagcacgac ggtataagcc
     3481 cctggactcc agttcttcag cctggcgggt caggcacaaa atctcccgcg ggtcgttagt
     3541 gccgacatag aaattgcgca caggtctggt ttcacgaact ggttgcggtt ccgcctcctg
     3601 cgctctctca gtcaggcgcg ggaaatgtct gcgtgtatcc ccttcacaac ggtgagccac
     3661 acgcccactc tgacgtaact tgcttgctga ctgcagaacg cgctgccgtg agtaacctgc
     3721 aaaagcatcc gcaatgtctc cggaagtaca ccccggatgg gcttcaatgt atttctgaac
     3781 ttcattcaaa agactcatga tcaccccctg aatcctgccg ggatctggct gtagtccacg
     3841 ttgtcgtaac tggctttgaa gtacgggtct tcgcgttttt ctgtgtacgt gctgacggac
     3901 ggcgataagc gcagggaaag ctcatcccat ttttcccgca gcttcgacgg gctgagcacg
     3961 ttacggcacc agaacggatc gcggctgacg cggctgtaca tctcgcagat ttgtttgtga
     4021 gtacgaccat cctgcacaca catcaggcga atttcgtttg cccaggctgt ccagttcggt
     4081 tctttgggac gaaccacctc gccgtcacat tcggcggcct gctcgtacag ggcgatgatt
     4141 tttttccaga gccactgtgc gcaggtcaaa tcatcctgcg ttccccactg gcgcttttta
     4201 gggctgaata caaccgcatc aggatggcga gttaaaaact cctgttcagc cgtctgcgtg
     4261 tccggttgcg aagcgtccgg acgagaagtt tttttatctg acggatcatg ttttgatttt
     4321 actgacggat ccccgccaga ttctgacggg tgaaaacccg cttttttgcc agatttcgac
     4381 gcatcaaatt ttgacgggtc agattttgat gcgtcagatt ttgacgggtc agaatctgac
     4441 agttgagaaa atgccgctgc ctgaagcttc gcaacgttaa gctgataaac attcgacgca
     4501 ttgcggttac cctggcgacg cgccttacgc gttaaccagc cttctgcttc cagccgtgcg
     4561 atagccgttc tgacggtact catccccgcg ccaatctgac gggcaatggt ttcaattgat
     4621 ggccagcaca caccttcgtc attactgaaa tcagccaggc gggccataat tgccacgctg
     4681 gataatttca tgcctgacgc tgcgcaacca tcccatacat agccggttaa tttagtgctc
     4741 atgaccgacc tctatttccc tgaatttacg acgaaactgt tcgagcgggc tgaagcactc
     4801 atgctcatag ccttcacgga ggtagataac ccgttgtgtt tccggctccc aacgaatgac
     4861 tctgacgggc actccgtagt gatctttgaa ccagcgatta acttgtcgca aaggactgtc
     4921 tccttctgcc ggttgaaatc ccccacagcc cactctgcaa agctgtgggt tacaatttcc
     4981 ctgtcacctg gtacattcac tgcatagcaa tactccacct tcgcttttcc acccggtaca
     5041 ggaagcgcaa tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt
     5101 ctccacagtc acgacacgcc acggcgcccg gagctgcaca ctcgcgggcg tcattacttt
     5161 ctgaaatgca aaaaattttg tagaccagtg ctgcatgctc ctgcagcttc gaaattgaga
     5221 ggtacagctc gtcgttaatt gctgtcttct catgcggttc cactacaccg tcttcgattg
     5281 ctgaacgaat ctgttttgaa taactgccga tctgttcaat gacttccagc agacgctggt
     5341 taatatcggc gttgtccaca tcctcgacgt caggaagaga cacaaagacg ccatttgcag
     5401 actgcgccac agcgtcagca atgaagtgag ttccaccagc acgttgcaaa atcattgccc
     5461 atcccagcgg gaaaatctga tcgccatcgg cacgaaggcg gttaaataat gcgttctctg
     5521 ttacatccag ccagtcagct gcttcagcgt aaccacccgg caactttgcg atagtttttc
     5581 tgacagcttt cacgtaccac tcaggctgtt tttctacttt ccagtgatgc ttacccacgg
     5641 ttagcctcat cgttctgtgg ttaaaaattg aaagtgttct gctaatcttt cggatagata
     5701 tccggtctta agtcagattt cgtaattgca cctgacgtgc attgctcaag ttttttagcc
     5761 agcacaaaac tggctttttt atagccattg aaaaccagcc gtaagtagcc aggtgttgag
     5821 ccaacttttc cggccaactc gccctgctgt tctttggtta aagagtccca atacgctttc
     5881 atacaatatg tacctccggt gtacatatta catgattaaa atgaaccttc aagatacttg
     5941 taccttaacg gtacaagggt tttaatttcg ttatgaaaac aatccatgac atccggcggt
     6001 ctaacgccag aaaactgaga gatggtgttg gcgggaattc ttcctttgcc actatgattg
     6061 atcgcgagcc aacccagacc agcaggttta tgggagatgg tgctactaaa aatatcggtg
     6121 acagcatggc acgacacatc gaaaaatgtt tcgacctgcc tgttggatgg ctcgatcaag
     6181 aacaccagac aacgaacatc acaaaaaaac ctgatgtttc aatcactaat aaacaaatca
     6241 cattagtccc tgtcatatca tgggtacagg ccggagcatg gaaagaagtt ggatattctg
     6301 aggttgattt gagcacagca gaaacgtatc cctgccctgt accctgtggg gaaatgactt
     6361 atatcttgcg ggtgataggt gattcaatga ttgatgagta ccgcccggga gacatgattt
     6421 ttgtcgatcc tgaagtacca gcctgccacg gtgacgacgt tattgcattg atgcacgata
     6481 caggtgaaac caccttcaaa aggttgatag aagatgggac acagcgttat ctcaaagcgt
     6541 taaacccaaa ctggcctgag ccttacatta agatcaacgg taattgctct ataattggaa
     6601 ctgtgatttt ctcaggaaaa ccaagaagat acaaaatcaa agcctaatca atgtttatga
     6661 acctgcttcg gcaggttttt ttatacttga caatgtaccc atgagataca taatgtaccc
     6721 aagagaaaca acgaacaggc aggacgccca cgaagtagcc gcctggggca tataaagtct
     6781 agggtgattc gttagcaaca aaaaagcgcc ctacaggacg cttagctctt taacaatctg
     6841 gtccccatca acaagtaact gataacttga ggaggtgtga aatgcacaaa acagaaccca
     6901 aaatcgtcgc gcccggatac acaaatgagg aaatttacga gtggatggca aagaagctgg
     6961 cagctataaa ccagcttcgt gaagtgctgt cttatcgaca ggaaacaata gactccttaa
     7021 aaaaactgga tcaggaaatc acggttttat cacaggatgt tactttagat attgtgcaga
     7081 caaattagga tcccattcat tttcgtcaaa atcatcaaag tgatgaattt gtgatctcca
     7141 gtctcgataa tctaaaaatt tctgggcggt tacgcttatt ttatcaagtg tgagttcatc
     7201 ctgaattgaa agaagaagtt catcaaattt catctcatta atctgttttg gcatccagtg
     7261 atgcttcatc agaataaggt gaaccagagc ctttttccca ttcaactgat tatagggagt
     7321 gccgaatttc ttccggtgct catgtaagac aaggtccaga agagtaagta atgttgccct
     7381 tgattccact ttgcttattt cgactgatga cactacccca ctgatttcaa tgccccgata
     7441 ctttccaaca ttttcacagt gggatttgta cagcgtgtag atattaccgg acatttcttt
     7501 tccttttgcg ttgttgggga taaccagatt aaccgaatcc ttgttgttgg gaaataacca
     7561 ggtccacctc gcctgatgtg gctaaaagca ggcacataac agctaagtat ttttaaccag
     7621 agagaatcct tagcgttgtg gtgaatgcgg ctcagcgcac gcgggttaag gttgaggctg
     7681 acagtcgacc ttctgtggat acccacccgt ctggtgtgca accttcgcca ggcaccggga
     7741 ggcacccggc accacaactt tatgctgtgt gtagtcctgg aggtaccagt ttgtaccctt
     7801 gcttccggct ggtaccgtcc tttttacaaa acagagaaga gcatcaccgg acgacgggct
     7861 cataacccaa tccatccggg cggctgccac cgcaggtgtt cttctctgtt ttgtggagaa
     7921 actaatcggc cttgcagggt cgatatgatg aggagcagca aaatggctag cgaacgcagt
     7981 actgatgtgc aggcatttat cggggagctg gacggcggcg tatttgaaac caaaatcggc
     8041 gcagttctca gtgaagtcgc ttccggtgtg atgaacacga aaaccaaagg taaggtctca
     8101 ctcaacctgg aaatcgaacc atttgatgag aaccgtgtga aaatcaaaca caaactctca
     8161 tatgttcgcc cgactaaccg cgggaaaatt tccgaagaag acaccaccga aacgccgatg
     8221 tatgtcaatc gcggtggtcg cctgactatt ctgcaggaag accagggaca gttactgact
     8281 cttgccggtg aacctgacgg aaaactccgc gcagcaggtc gttaatatcg tttttaatta
     8341 actgattatt tatctcatca ctgaatatct ttatatagtg aggacttatt atgtctcaga
     8401 acttagacgc aaccgcaatt aatcaaatcc atgcccttat ttctgctcag ggtgttaatg
     8461 aaattatcag taagattggt gccgatgctg tggcattgcc tgagaatttc cgcattcatg
     8521 atctggaaaa atttaattta aatcgcttcc gtttccgtgg tgcgctttcc actgccagca
     8581 tcgatgactt tacccgttat tctaaagatc ttgcagatga aggcacccgc tgctttatcg
     8641 atgctgataa tatgcgtgcc gtcagtgtgc ttaacctggg tactattgat gaaccaggtc
     8701 acgcagataa caccgccaca ctcaaactga aaaagacagc accgttctct gctctgttgt
     8761 ctgttaacgg cgagcgtaac tcccagaagt cactggcaga atggattgaa gactgggccg
     8821 actatcttgt gggctttgat gctaatggtg acgctattca ggcaacaaaa gcggctgcgg
     8881 ctgtccgtaa aatcacgatt gaagcaaacc agaccgctga ttttgaagat aatgacttca
     8941 gcggcaaacg ctccctgatg gagtctgtcg aagcgaagac caaagacatt atgccagtgg
     9001 catttgaatt taaatgcgtt ccgtttgaag gtctgaaaga acgtccgttt aaattacgcc
     9061 tcagtattat cactggcgat cgtcctgtac tggttctgcg cattattcag ctggaggcgg
     9121 tgcaggaaga aatggctaac gaatttcgtg atctgcttgt tgagaaattc aaggacagca
     9181 aagtagaaac ctttattggt actttcaccg cctgatttca ttactgcaaa tgcccctgcg
     9241 ggggcattta tggaaacgta atttactcaa taatcgccgg atggtgaggg attcttttta
     9301 ccagaattca gcgcggtgca gcgcatatac gtggagaaca aaatgtcatt tattaaaact
     9361 ttttccggga agcattttta ttatgacagg ataaataaag acgacatcga tattaacgat
     9421 atcgcggttt ccctttcaaa tatctgtcgc tttgccggtc atctttcgca cttctacagc
     9481 gtcgcccaac atgcggttct ttgcagccag ttggtgccgc aggaatttgc ttttgaagcg
     9541 ttaatgcatg atgcaacaga agcgtattgc caggacattc ccgcaccact gaaacgcctt
     9601 cttcctgact ataaacaaat ggaagaaaaa atagacgccg taatccgtga gaaatacggg
     9661 ttacccccag ttatgagtac gcccgtgaaa tatgccgatc tcatcatgct ggcaaccgaa
     9721 cgccgcgatc tcgggcttga tgatggctct ttctggcctg tactggaagg tatcccggca
     9781 acagagatgt tcaacgtgat tccactggca ccgggccatg cctacgggat gtttatggaa
     9841 cgctttaacg agttatcgga gttacgcaaa tgcgcatgaa tgttttcgaa atggaagggt
     9901 ttcttcgtgg gagatgtgta ccgcgagatc tgaaagtaaa tgaaacagat gctgaatacc
     9961 tggtgcgtaa attcgatgcg cttgaagcta aatgtgcagc acaggaaaac aaagtaatac
    10021 cagtgtcaac tgaactgcca ccagcaaatg aaagtgtttt gttattcgat gctaacggag
    10081 aaggctggct aattggctgg cgttctctct ggtacacctg gggacaaaaa gaaaccggag
    10141 aatggcagtg gacatttcag gtcggggacc ttgaaaacgt caatatcact cactgggcag
    10201 taatgccaaa agcaccggag gctggagcat aatgaccact tttaccgaca aagaactgat
    10261 taaagaaatt aaagagcgta tcagcagcct tgacgtgcga gacgatattg agcgccgtgc
    10321 ttatgaaatc gcactcctat ctctggaagt agaaccagat gaacgcgaag cttatgaatt
    10381 attcatggaa aagcgtttcg gtgacttagt agatcgtcgg agagcaaaaa acggcgataa
    10441 cgaatacatg gcatgggata tgactctcgg ttggatcgtc tggcagcaac gagctggtat
    10501 ccatttttca acaatgtcac agcaagaggt gaaataatgg agccatacag cctcacactc
    10561 gatgaggcct gtcattttct caagatatcc agaccgactg ccattaactg gatacgcaca
    10621 gggcgtcttc aggcaacacg caaagatccc actaagaata aatctcctta cctcacaaca
    10681 cgacaagcct gcattgcggc tcttcagtct ccgctgcata ctgtccaggt gagcgcgggt
    10741 gatggcataa cagaggaaag aaaatgtcac tcttccgcag aggtgaaata tggtacgcca
    10801 gtttcacatt gccgaacggt aaaagattta aacagtctct tggaacaaag gacaaaaggc
    10861 aggcgacaga actccatgac aagctaaagg ctgaagcatg gcgggtcagc aaacttggtg
    10921 aaatacctga tataacgttc gaggaagcgt gtgtcaggtg gcttgaagag aaagcacata
    10981 aaaaatcact ggacgatgac aaaagccgga tcggattctg gcttcaacat ttcgcaggaa
    11041 tgcaactaag agacattact gaatcaaaaa tttattcagc aatgcagaaa atgacgaacc
    11101 ggcgtcatga ggaaaactgg aaactcaggg cagaagcatg cagaaaaaaa gggaaacctg
    11161 ttccagaata cacgccaaaa ccagcgtccg ttgcaacgaa ggctacgcat ctttcattta
    11221 taaaggccct actaagagcc gcagagcgtg aatggaaaat gctggataag gcaccaatta
    11281 ttaaagtgcc tcaaccaaag aataaacgga tccgctggct ggagccccat gaagcacaaa
    11341 ggctgattga tgaatgtccg gagccattaa agtctgttgt tgaatttgca ctggcaacag
    11401 gcttaagacg ctcgaacatc atcaaccttg aatggcaaca aatagatatg cagcgccggg
    11461 tggcatggat aaacccggaa gagagtaaat caaaccgcgc aattggcgtt gcgctgaatg
    11521 atactgcatg tcgcgtattg aaaaaacaaa tcgggaatca tcaccgttgg gtatttgtgt
    11581 acaaggaaag ctgtaccaaa ccagacggaa cgaaagcgcc aacagtaagg aagatgcggt
    11641 atgacgcaaa cacagcctgg aaagcggcgc tgagacgggc tggtattgat gatttcagat
    11701 ttcacgactt gagacacacc tgggcaagtt ggctggttca agccggagtc ccgttgtcag
    11761 ttttacagga aatgggaggc tgggagtcta tcgaaatggt tcgtcgatat gctcaccttg
    11821 cacctaatca ccttaccgaa cacgcacggc aaatagactc gatcctgaac ccatcggtcc
    11881 caaatttgtc ccagtcaaaa aataaggaag gtactaatga tgtgtaactt attgatttta
    11941 atggtgccga taataggagt cgaacctacg accttcgcat tacgaatgcg ctgctctacc
    12001 aactgagcta tatcggccct gaaaggacat gttcacgaac gtgaatcacg gtggacaagg
    12061 ttaaaactaa ccgggcgatg cgtcaatggc cttgtgaatc aaatggctac ttttgcatca
    12121 cccggtttta tttacgcacg aatggtgtaa tcaccaatgc cgatccactt gtaagtggtc
    12181 agtgcttcca gccccattgg gccacgcgcg tggagttttt gtgtgcttac cgccacttcc
    12241 gcacccagac caaactggcc gccgtcggta aaacgcgtag aggcgttaac gtaaacagcg
    12301 gacgaatcca cttcgttaac aaaacgctgg gcgttgcgca tatcgcgggt caggatcgca
    12361 tcggagtgtt gtgtgccgtg ttcacgaata tgggcgatgg catcgtcaag atcgctgacg
    12421 attttgacgt tcaaatctaa tgacagaaac tcatcgtcat actcttcggc tttaaccgcc
    12481 accaccttcg cagggcctgc ctgcaactgt gtcagcgcag ctgcatctgc gtgtaatgtc
    12541 acgccgcttt ccgccatttg tttgcttaat gcgggcagga agctatcggc gatgttttta
    12601 ttcaccagca acgtttcaac cgtattacat gtgctcggac gctgagtttt cgcgttgacg
    12661 atcactttta atgcttcagc gatctctgca ctttcatcaa cgtaaatatg gcatacgcct
    12721 ataccacctg tgatcaccgg gattgtcgac tgttcacggc acagtttatg caaaccagcg
    12781 ccaccacgcg ggatcagcat gtcgatgtat ttatccatac gcagcatttc actgaccagc
    12841 gcacggtcag gattatcaat cgcctgcacg gcacccgccg gtaagccaca ggatttcagg
    12901 gcgtcctgaa tcaccgccac cgttgccgcg ttagtgcgac aggtttcttt accaccacgc
    12961 aggatcaccg cattaccggt tttcaggcac agcgaagcga catcaaccgt cacgttcggg
    13021 cgcgcttcat aaatcacgcc aataaccccc agcggtacgc gacgacgctc aagacgcagg
    13081 ccgctgtcca gtacgccgcc atcgattacc tgccccaccg gatcggcgag gttgcacacc
    13141 tgacgtacat cgtcggcaat gcctttcagc cgtgcgggcg tcagtgccag acggtcaagc
    13201 atcgcttcgc taaggccatt ggctcgcgcg tcagcaacat cctgggcgtt agcgttgagg
    13261 atgatttcgc tttgtgcttc cagttcatcg gcgatttttt ccagcacgcg atttttttcg
    13321 cggctggaga gttgcgctaa tttatacgag gcttgcttcg cggcaatgcc catttgttcc
    13381 agcatcggcc agctccttaa cgggtaatca tgtcatcacg gtgaacggca accgggccgt
    13441 attcatatcc cagtattgca tcaatttctt gcgagtggtg tccggcaata cggcgtaatg
    13501 catcgctgtt gtaacgactg acaccgtggg cgatatcgcg accttcgagg ttgcaaatgc
    13561 ggatgacttc accacgcgag aaattgccag tcacgctttt aatgcctttc ggcaacaggg
    13621 agctgccgcg ttccagaatg gcggcagttg ccccttcatc taccgtgatt tcacccgccg
    13681 gcggcgcacc gaaaatccag cgtttacggt tttcaagcgg agtcgcctgg gcatggaaca
    13741 gcgtaccgac ggaaatgcct tccatcacat caccaataac gcccggcttg ctgcccgcgg
    13801 caataatggt gtcgataccc gcacggcaag ccacgtcagc ggcctgcaat ttagtactca
    13861 tgccgccagt tccgaggcct gaaacgctgt caccggcaat cgcgcgcagt gcgtcatcaa
    13921 tgccgtaaac atctttaatc agttctgcct gcggatttct gcgcggatca gcggtataca
    13981 aacctttttg atcggtcagc agcaacagtt tatcggcacc cgccagaatc gccgccagcg
    14041 cagaaaggtt atcgttatcg ccgaccttaa tctctgccgt agcgacagca tcgttctcat
    14101 tgattaccgg aacgatattg ttatcgagca acgcacgcag ggtgtcgcgg gcgttcagga
    14161 agcgttcacg gtcttccata tcagcacggg tcagcagcat ttgcccgacg tgaatgccat
    14221 aaatcgaaaa cagctgttcc cacagttgaa tcagtcgact ctgccctacc gccgccagca
    14281 gttgtttcga ggcgatagtc gctggcagtt ccgggtaacc caggtgctca cgtccggcgg
    14341 cgatcgcgcc cgacgtcaca ataacaatcc gatgcccggc ggcatgtaac tgcgcgcac
//
LOCUS       DAJVPP010000095        14190 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVPP010000095 DAJVPP010000000
VERSION     DAJVPP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.095"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..562
                     /locus_tag="OQM34_003094"
     CDS             <1..562
                     /locus_tag="OQM34_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCS1453442.1"
                     /translation="TFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVDEVE
                     HLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERT
                     PGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCL
                     QKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(624..1346)
                     /locus_tag="OQM34_003095"
     CDS             complement(624..1346)
                     /locus_tag="OQM34_003095"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1453443.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1348..2519)
                     /locus_tag="OQM34_003096"
                     /pseudo
     CDS             complement(1348..2519)
                     /locus_tag="OQM34_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2546..4162)
                     /gene="bglH"
                     /locus_tag="OQM34_003097"
     CDS             complement(2546..4162)
                     /gene="bglH"
                     /locus_tag="OQM34_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCS1453444.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4248..5642)
                     /gene="bglB"
                     /locus_tag="OQM34_003098"
     CDS             complement(4248..5642)
                     /gene="bglB"
                     /locus_tag="OQM34_003098"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCS1453445.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5661..7538)
                     /gene="bglF"
                     /locus_tag="OQM34_003099"
     CDS             complement(5661..7538)
                     /gene="bglF"
                     /locus_tag="OQM34_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCS1453446.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPKNDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7671..8492)
                     /gene="bglG"
                     /locus_tag="OQM34_003100"
     CDS             complement(7671..8492)
                     /gene="bglG"
                     /locus_tag="OQM34_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCS1453447.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKWLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQYAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8778..9503)
                     /gene="phoU"
                     /locus_tag="OQM34_003101"
     CDS             complement(8778..9503)
                     /gene="phoU"
                     /locus_tag="OQM34_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS1453448.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9518..10291)
                     /gene="pstB"
                     /locus_tag="OQM34_003102"
     CDS             complement(9518..10291)
                     /gene="pstB"
                     /locus_tag="OQM34_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1453449.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10383..11273)
                     /gene="pstA"
                     /locus_tag="OQM34_003103"
     CDS             complement(10383..11273)
                     /gene="pstA"
                     /locus_tag="OQM34_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1453450.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11273..12232)
                     /gene="pstC"
                     /locus_tag="OQM34_003104"
     CDS             complement(11273..12232)
                     /gene="pstC"
                     /locus_tag="OQM34_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1453451.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12319..13359)
                     /gene="pstS"
                     /locus_tag="OQM34_003105"
     CDS             complement(12319..13359)
                     /gene="pstS"
                     /locus_tag="OQM34_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1453452.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13607..>14190)
                     /locus_tag="OQM34_003106"
     CDS             complement(13607..>14190)
                     /locus_tag="OQM34_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1453453.1"
                     /translation="YIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGD
                     ITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDD
                     ISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSG
                     SVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3638 a         3641 c         3248 g         3663 t
ORIGIN      
        1 tacattcaaa tatcatccca agccactgga aacaggtgca tttgaacagg ataaaaccgt
       61 agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct tttattgcgt
      121 tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg cagaaaagtt
      181 tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt atgatgaaga
      241 ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag agttggttga
      301 acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt gtggcgattt
      361 ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg atgaatttgc
      421 caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag ctaaatgcct
      481 gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg gcaagctgag
      541 actgtggggt gatttttcgt agttatttaa ataatgagaa caggccggag cgtaattcat
      601 acatccggcc ttatttcttc agcttaaccg aacgctaatt ctgcggcagc ggctttatca
      661 gcgattacca tcagcgaagg atgcagctgc aacacggaag ctggaacatc ttcggtaacc
      721 ggaccttgca gcacattttt cagtgcctgc gctttacctg cgccactgac aattatcagc
      781 aagtttttcg ccgccatgat gcttttcggc cccatcgtca cgtagctgtc aggcactaac
      841 gaaaagtcgc cgcccaactc accatgcgcc acaatatcaa ccatctctcc ctggatcggg
      901 aactccacgg tttgctcatg gaaatgggtc gtattcggca aattgccaca aaaatgacca
      961 tctgcaccta atcccagcac caccaaatcc aggccgcctt cacgcgccag tttctgatca
     1021 tgctcgcggt agttatcaat agtgagcttc tggatattct cttctttgat ccccgcaggg
     1081 gtgaaaaaca gattacgcag attggtaatc gttacgcctt ctccctcttt gccgcgaaag
     1141 ggaatttcat cgaaattata gaaatagcag ttatcgtacc aaggcttacc tttaaccagg
     1201 gtggtgaggt attcatacat gcctttgggc gtgctaccgg cggtaattgc caggttaaca
     1261 cgacgcgtct tcgacatata accaagcaga tggtgtgccg cgacacggct catttcctgg
     1321 taatcttcgg taatgattaa tttcatctta atgccttatt ttaaatttct gtacgaaggc
     1381 tgcataggca ggccgccaga catccatttc atggttcagg ccgggatatt cctggtaatc
     1441 aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct tgccggttac
     1501 gacatcttta tcaccaacga ccacagtaaa attacgtagt tgctggttaa tagctgccgg
     1561 atcgttcagc cgggccgcga caccttcatc cggtacggtt gtcgtggtaa caccactgaa
     1621 tgtggccagc caaagctttc cagatgattc attccggaaa ccagcgcctg gtacccgcct
     1681 tgtgaaagcc ctgccagcgc gcgaccatcg gcatctttac ggacactaaa acgcttgcta
     1741 atcagcggga taatatcgtt catcagttcg cgatctgccg ctttagcatt cagcggataa
     1801 aagactttac gtctttcctg aggcacgaaa tcttcgggaa taatgccctt cgcatcggtt
     1861 tcggtatccg ggatcaccac cagcatcggt ttaattttcc cttcagccag caggttatcc
     1921 atgatttgcg ggatacgccc ctgatcgata gcggaacgtc cggtatcacc aaaaccgtga
     1981 tagaaataga gcactggcaa aggctcgccc atgccggtgt atcctggcgg ggtccagaca
     2041 tacatctgac gttcagattg caatgcgttg gagtggtaag ttatggcgat caaatcacca
     2101 tgcgcaacag aacgcgtatc cagataactg cctggcacca gaatcatact ggagttaacc
     2161 tggcgctgag gcttagtcat tgcggtgcct gtatcaatgc tgcgtacacc atcaacattg
     2221 aaaaaatact cgtacaggtt gcctttcagg ataggtgtgc gccacgacca gactcctgct
     2281 tcgtctttgg tcatcgggtg aatattgtcc ggaaccggaa cacccaccac tacagagaca
     2341 ttttttgccc caggggcaaa gtagcgaaag gtcacgctgt tatcggcatt gacctgagtc
     2401 acatactgtt tcaccgggat agtgggtgcc gggctggctg gcatatccgc tgctatggcc
     2461 cactgagcag aaataccgct ggcaatagcc agcgttaaag cagcaatttt tatattcatc
     2521 gtaatattcc tttcattaag ttccattacc accagatttc agcctgggca ccgacagcaa
     2581 actgggcgtc tttattgtct tcgaaggtga agccatccag ttcgttttcc agggctttga
     2641 tatacgtggc gtagaaacga atttccagac gcgaggtcaa catactggta ttgactttaa
     2701 aggtatggaa gagcgtggtt ttataaccag actcattaaa tttattacta ttcgcatctt
     2761 tgttttgctg ggtgaaatag cccagttcaa cacctgtctg gttatattgg tcccaaatat
     2821 aggccgggcg aacaaccgca cgaatagatt cgaaatcaga gtgtgcgcct gtttcgtagc
     2881 tataaatatt gttaccgaag gagtaaacaa tcgcgttagc cacaataaaa tgatcgccga
     2941 tataggcttc gccctgcgag gtcagacgaa ctgctgttcc gccggtgtga tcaccataat
     3001 aacgaccatt aaaggtggta aatggacttg cgccagcata acggccaaag ttcctggcga
     3061 tagagttatt cgcaaccagg aaggagaatt cgttgaagcc acctttatca aatttctgcg
     3121 ttaaagatgt gccgaacatc caggtatctt tccagtcata atacccgtta ttatcctgat
     3181 tatctttttc cgatgcgctt tcgtttgccg tgacataacg accactcacc attaaggtcg
     3241 ctttatccca taacgggata tctttatagc gtaaatcaat agtatgggta ttaatctgct
     3301 gtttgttttg caggctgcga tcgtaatcat cgacatcttc gcgaaccagc gcgatatcaa
     3361 ttttacccgg accgactttc cagttttcca gacctacacc cgctgcagca tcagtacgct
     3421 gcgttttcca gtccagcatc tggatttcaa ttttcggcgc accgtgttta cccacccaga
     3481 aatcagcctc tggcgcaaag ggcaggaaac ctttggtggt aacgtacata tcggagaact
     3541 gcataaagtt ctcgccaccg gcattatcgc caaaccagcc ggtagagtac tgctgaccaa
     3601 cgttaccatc tatcatcaca acggcatcaa cccgtttgcc gttttcgttg tagacacgtt
     3661 gttttaactg caaatcaaac cagccggagt attcgttacc aaagcggccc agagaaccaa
     3721 tcgcccatga tttaggtgaa ccatgagagg cggtccccca accagaacgg taatagccgt
     3781 tgtaactaaa gccaatttca tctttcacaa acttactgaa atctttcaga gtcatggaag
     3841 aataaatcga gcctgtctca ctggcatttt tctcttcatt tttgaccagt acagcatcag
     3901 gttttgcggc actactggtc gggaacggat tggcggcata cccttgatca ttcgtactta
     3961 cgctacgatt caccgtcgct ggcgtatatt ttttcttctc ttcatcttta tactttttga
     4021 gttcgctttg cgtttctctt aacgcttttt ctaataactc aaggcgttgt tccaccgtta
     4081 atgattcagc caatgattgt gcagaaaagg ctaacggtgc cattaataag atggcagagg
     4141 taataagatt tcgtctaaac ataatatccc tttatggtgc aaagaaagaa ttaacgtatc
     4201 gcatccagac tgttctgaat gcgacgataa ttaaggtgct ttattggtta tttttttaat
     4261 gacagccccc gcgttttgat cacttctgca taccatccga agcttttctt acgcgttctt
     4321 gtgaggcttc cttcgccatt atcatcacga tccacataaa taaagccgta gcgcttagac
     4381 atttgtgaat gagatgcact gactaaatca attggccccc aactggtgta ccccataata
     4441 tccacaccat cggcaatcgc ttcatttacc tgtaccaggt gatcgtttaa ataggcaatt
     4501 cgataatcgt cctgtatcga accatccgct tcaacgctgt cttttacgcc taatccattc
     4561 tcgacaataa ataacggttt ttgataacga tcccaaagcg tatttaacag aacccgtaat
     4621 ccaaccggat caatttgcca cccccactct gaacttttca gatgcggatt ggggatcata
     4681 ttcagtatgt tgccctgcgc atttttatta atgctttcgt cgtgggaaac acaaccagtc
     4741 atgtaataac taaaagagat gaaatctaca gtatgtttta aatcttctgc gtcactttca
     4801 gtcatctcaa tggtgatatt gtggtcgcgg aagaaacgct gcatatagcc gggatattgg
     4861 ccacgcgcct gaacatcacc aaagaacatc cagcgccggt tctcttccat ggcctgcaac
     4921 atatcctgtg gctggcaggt gagggggtaa accagcccac cgagaagcat attgccgatt
     4981 ttcgcttcgg ggatcaggct atgataggct ttaactgccc gcgcactggc aaccagttga
     5041 tgatggatcg cctgataaac ttccgcctcg ccactctctt ctgccagccc cacgcccgtg
     5101 aatggcgcgt gtaacgacat attgatttca ttaaacgtca gccataacgc tactttatgt
     5161 tggtagcggg taaagaccgt gcgggcgtaa tgctcgaagt gatcgatgac cgcccgatta
     5221 gcccaaccgc cgtagttttt caccagccca tatagcattt cgtaatggga taacgttacc
     5281 agcggcttga tccccgcctg cgccatttca tcaaacagcc gatcgtaaaa cgctaacccc
     5341 gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc aatggaaata
     5401 cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta acggtaataa
     5461 aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc catttttccc
     5521 attacgccat gaggctgtaa atctgaggtc gtgatccctt tgccatcttc cagccaggca
     5581 ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg aaatgctttc
     5641 ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc tgcgcttatc
     5701 tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat taataccggc
     5761 gtcgtcagat caaatccggc ctcgcgaata gcagggatat caaaagaaat cagccgatcg
     5821 cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc gtccagtttt
     5881 acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc aatgccaatg
     5941 gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg cacttcacca
     6001 accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt ggtatcagcg
     6061 acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc ctgctcaggt
     6121 ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg acgtttactg
     6181 gtgatgaaat gaagcatcaa cgtaccaaca aatgcgcaac ctatggcaat gacaccgcca
     6241 ataacgctgg cccagacggt gaaatcaatc cccgttgacg ggatggtctg catgaaggtg
     6301 aaaatacttg gcaaaccaaa ggagtaaact ttcgtttgcg cgtagccaat gatggtggcc
     6361 cccaatgctc cactgataca ggcgataaca aaggggtact tacgcggcag gttgacgcca
     6421 tataccgctg gttcggtgat accaaacaga cccgtcaacg ccgctgatcc cgccaccact
     6481 tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac ctgcgccata
     6541 atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa gttattgata
     6601 cacagcggca ccaggcccct gtgcagtccg aacatgacga agatttgcca gaagccgccc
     6661 attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc ggcggcaatc
     6721 atttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg tgtgataacc
     6781 atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca cgcattaagt
     6841 cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac cgatgacgag
     6901 taattcaaca atgtgaccgg aatacccagg aaatccagcc ccggcgcatc cgctttttgc
     6961 ccgttctcga aggcagtcag aattaatgga tgcactaacg ctccaccaat caccatggca
     7021 gtaaatggat taccgccgaa gcgtttgccc gcggtgtatc ccaggattat cgggaagaac
     7081 ccaaacaagg catcactggc gctgaataaa attaaataag taccactttg ttcggtcgtc
     7141 cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc cgccatcaga
     7201 ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag cagattacct
     7261 ttctcatcat ttttcggtgc ctgttgcgct ttttcgccaa ggcctgccac gctgttaacc
     7321 gccaggaaga catcggccac atggttacct atgaccacct gaaactgacc accgctttcc
     7381 accaccataa taataccggg ggtctttttc agtacctcag cttgcgcttt gctttcatcc
     7441 tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac tcacaatgtt atctgcgccc
     7501 ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct accgctttgc
     7561 ggcaaaactc caaaaaaaac ctgaaaaaaa cggcctgacg tgaatcaagc aatttttttc
     7621 aggttttgcc cgcttagtgc ggtaacaatc ctttactcag taataatatt tcagtgttct
     7681 ttgcgcacgc gctctatatt tatggctaaa aacataatct ctgcgggtga aattttacgt
     7741 tgatactgca aaccaataaa aatggcgatc cgttccgcat attgccatgc ttgcgggtaa
     7801 ttttgtttta ctgcttgttg taatgattca tcactatcgt taattgaagc atgttcaaga
     7861 atacgccagg ataaaaactt cagatgtgta accagtcgct gataactcaa gctttcttcc
     7921 tggtaattaa ggctgaactg aaattttatt aattgcagca tttcgcgcat taactgcgtg
     7981 acacctgcaa catcctccat atttccgctc atttgggcac tgaccagatg catggcaata
     8041 aagcccactt catctttcgg taactgcacg cccaaccatt tatcaataat ggttaacgct
     8101 tcttccccta gctggaactc tttcgggtaa agccgctgga tatcccacag caacacgttt
     8161 tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta gcgagatata aatactgtcc
     8221 tgtaattttc ccaagcgctc ctgcgctaaa gagataagac gatcacaggt tgccatcacc
     8281 tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt tcagttcatg actgctcaag
     8341 gcatactctt tttctattcc acttgagtta attctttcgc cagcgcgttt ttgaaagcca
     8401 attccgcgcc ccatgacgac tttttcccgc tgttgatcat caataaccac cacaacatta
     8461 ttgttgagaa ttttggtgat ttgcatgttc atagcaagga ccttttttat aaacaaaaaa
     8521 aaccgacttc accagtattc tctggttatg tcaggttttg cctgcgaatg cagtaacaat
     8581 ccagtcattt attaatggtt tttataacga acatccaggt tcggaaatta atttaattgc
     8641 gtgcagagaa aatagcaatg cgctattgat aaaaatatga ccatgctcgc agttattaac
     8701 tttgtgtaat tttaggaatt tataaagtta tatataacaa atcccaataa ttaagttatt
     8761 gggatttgtc tggtgaatta tttgtcgcta tcttttcccg ccagcagttt atccagctca
     8821 tcaccgccta catgacggaa atcctgaccc ttcacgtagt agaagataaa ctcacaaata
     8881 ttctggcagc ggtcgccgat acgttcgatt gaacgcgcac agaacagcgc ggtaagtacg
     8941 ctcggaatag tgcgcgaatc ttccatcatg taggtcatca gttgacgaac aataccttcg
     9001 tattcctgat cgactttttt atcttcacga taaatacgta ccgcttcgtc aatgtccatc
     9061 cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg tatgacggcc cagcgactcc
     9121 agacttacca gcaacggctg atgctgctgg gagaatttct ccagcgcagt acggcagatt
     9181 ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg ttttactgat caccataacc
     9241 agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa tgcgcacgca ggcttcatcg
     9301 atcgccactt ccatcatgtt gacgttcttg tcgccttcga tcacgcgctt cgccagatcg
     9361 ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct gctgctccac catgccgccc
     9421 atggtcatca cctgcgtgcg gatactttcc agttcggcgt tgaactggcc ggaaatatgt
     9481 ttattaagat tgagactgtc cataacgcac tcctgaatca accgtaacga ccggtgatgt
     9541 agtcttctgt ttgtttcttc gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa
     9601 tcaattcgcc caggtacata aatgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt
     9661 tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac
     9721 gcccggtaga gatggggtcg agcgccgaac acggttcgtc gagcagcagc acttccgggc
     9781 gaatggcgat accacgcgca atacacagac gctgttgctg accaccagag agagagtaac
     9841 cgctctggtg caatttatct ttggtttcgt tccacaatgc ggctttggtc aatgcccact
     9901 gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa cagacgaacg ccaaaggcga
     9961 tgttgtcgta gatggacatc gggaacggcg tcggtttctg gaacaccatg cccactttcg
    10021 cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt gtcgccatca agcagaattt
    10081 caccttccgc acgctgctcc gggtacagtt caaacatttt gttgaaggta cgcagcagcg
    10141 tcgatttacc gcagccggac ggcccgataa acgccgtaac ctggtttttg gcgatatcca
    10201 agttgatgtt tttcagggca tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa
    10261 ttttactcgg ggcagtttca accatactca ttgcaatctt ttcctcatcg tacctgatgc
    10321 ttttcgctaa tcaggcttac cgtagggccg ggtaaggcgt tcacgccgca tccggcaaag
    10381 tgttaaccgt gtttattctt cgcaaaaaca acgcgcgcca gaatgttcag cagcaggacg
    10441 cacagggtaa tgatcaatac cccggcccag gccagttgtt gccattccgc aaacgggctc
    10501 atcgcaaact taaagatcgt caccggcagg ttggcgatcg gctgcatcat gtccgtgctc
    10561 cagaactggt tggagagcgc ggtaaacagc agcggcgcgg tttcaccggc aatacgggca
    10621 atcgccagca ggataccggt cataatcccg gacaccgacg ctttcagcgt aatcgcggag
    10681 atcatcttcc acttcggtgt acccagcgca taagccgctt cacgcaggct gtccggcacc
    10741 agtttcagca tgttctcagt ggtgcggata acgatcggca cctgcaacag cgccagcgca
    10801 attacgcccg cccagccgga gaagtgctcc atctgtgcca ccacgatggt gtagacaaac
    10861 agaccaacca caatcgacgg cgcggagagc agaatgtcgt taatgaagcg aatcacttct
    10921 gccagccagg atttacgacc atattccgcc agataaatcc ccgccataat gcccagcggc
    10981 gtaccgaata ccgtggccca caaaattaac agcccgctac ccgccagggc gttcgccaga
    11041 ccaccacctt ccgtattggg cggcggcgtc atttcagtga acagcgccag cgacataccg
    11101 tcgataccgc gagtgatggt ggacattaaa atccagatca gccagaacag gccgaaggcc
    11161 atcgtcgcca tcgagagcgt cagcgcaata cggtttttga ggcggcgacg cgcctgcatt
    11221 ttgcggcgag attcagccag cgccgcagtg gtttgcattt caaccatagc cattagcgtg
    11281 ccccctcatt cttagccagg cgcataatca taaacttcga tgcggcgagg acgatgaagg
    11341 taatcacaaa caggatcagg cccagttcca tcagtgcggc aacgtgcaga ccggattccg
    11401 cttccgcaaa ttcgttcgcc agcgcagagg tgatactatt gcccggcatg tacagcgagg
    11461 cgctgtcgag ctggtaggtg ttaccgatga taaaggtcac cgccatggtt tcaccgagcg
    11521 cgcggcccag ccccagcatg atgccgccga taacaccatt tttggtgaac ggaagaacga
    11581 tacgccagat aacttcccag gtggtgcagc caataccgta ggccgactct ttcatcatca
    11641 ccggggtttg ttcgaacaca tcacgcatta ccgccgcaat gtacggaata atcatgatgg
    11701 cgaggatcac gcctgccgcg aggataccga taccaaatgc ggggccagag aacagcgcgc
    11761 caacaatcgg gatattcgac atgatattgc cgaccggctc ctgaaagtaa acggcgaaca
    11821 gcggcgcaaa gataaacagg ccccacatgc cgtaaacgat acttggaatg gctgccagca
    11881 gctcaatggc gatacccagc gggcgtttca gccagccagg cgcaagctca gtcaggaaca
    11941 gggcgatacc gaaactcacc gggacggcga tcagcagcgc gataaacgaa gtcaccaacg
    12001 taccgtagat cggcaccagc gccccgtaga tatcgttcgg tgcatcccac tctttggtcc
    12061 ataggaaagc cagaccaaat ttctgaatgc tcggccagga ggagatgatc agagagacaa
    12121 taatgccacc caacatcaat agcacaatca gcgccgccag ttttaccagc acgctgaaaa
    12181 ttatgtcgcc ctttttaccc ggtgggttaa aagcgggctt ggttgcagcc ataagttact
    12241 cttcagttaa acgcgtttac aaagtcattt tgtaatgccg gatgcggcgt aaaacaccgt
    12301 acccggcctg gagttttatt agtacagcgg cttaccgcta ctgtctttaa tattggtctt
    12361 ccacgcagcg cgaacctgtt caactacact atccggcagg ctggcgtaat ccaggtcgtt
    12421 cgcctgttta gccccggttt tgtacgccca gtcgaagaat ttcagcacct ctgtgccttg
    12481 ttctggtttc ttctgatctt tgtggatcag aatgaacgtg gtagaggtaa taggccatgc
    12541 atcttcgcct ttctggttgg tcagatcctg agcgaaggtt ttgctccagt ctgcaccttt
    12601 tgctgcatta gcgaagtttt cttcggtcgg actaaccggt ttaccatcag cggagatcag
    12661 tttggtgtac gccaggttgt tctgcttcgc gtaagcatat tcaacataac caattgcacc
    12721 cggcagacgc tgaacgaacg cggcgatacc gtcgttacct ttaccgccca gaccgatcgg
    12781 ccattttacg gtagagccag taccaacgtt gtttttccac tcttcgttca ctttcgccag
    12841 gtagctggtg aagacgaagg aagtcccgga gccatctgcg cggcgtacta cagcaatgtt
    12901 ttgtgaaggc agtttcagac ccggattcag tttggcgatg gcttcatcat cccacttctt
    12961 gattttgccc aggtagatgt cgccgagggt tttaccatcc agcaccagtt cgccagactt
    13021 cagccctgga atgttaaccg ccagcaccac gccgccaatc acggtcggga actggaacag
    13081 accttcctga gccagttttt cgtcagacag cggcgcgtca gaggcaccaa aatcaacggt
    13141 attagcgata atctgtttta cgccaccgga agaaccgata ccctggtagt taactttatt
    13201 accggtttct ttctggtaag tgtcagccca tttggcatac accggcgcag ggaaggttgc
    13261 acctgcacct gtcaggcttg cttctgcaaa cacagagaaa gcactcatcg ataaggtcgc
    13321 ggcgacaaca gttgcgacgg tggtacgcat aactttcata atgtctcctg ggaggattca
    13381 taaagcattg tttgttggct acgagaagca aaataggaca aacaggtgac agttatatat
    13441 aaggaatatg acatttttat gacagaaagg aattaccaaa taacttactg attttaaatg
    13501 tgaagatgaa aatcccacat gccatctttt tgtcatgatg catttaaaag tgataaagaa
    13561 atgggtgaga tgacaacgat gtgatgaaca atcgttgtca gacctattag cgcacaatct
    13621 caacagttat cgtcgctgtt gcagtaaatt gccccggggc tgggcgagct ccagtaaggc
    13681 tcgcaggagc agctgaaaaa gttacactac ctgatttttc accatacact gttgatttat
    13741 ccatgtctgt tggcaactca ccaccgttca ctttgatttc cttattattt ttgtcgtaaa
    13801 tttttatccc cacgttagag tttgaagtta cgaccattga attgtccgta ttgggttcgg
    13861 tagcataaaa agacgtactg atatcatcgg tattcagtat ccccaggttt tcacactcaa
    13921 tagtgactgt tttagtaatt ttatgtgttg ttaccgcact tccaacaaca gatgaaaaat
    13981 ctgcagctgg tatttcttta aaatcaaaac aaactggctt taaattattt atttcgcaac
    14041 tttgcggagc tgtaatatcg ccccgaatat aaacatcagc catcggctgt gagtaatcca
    14101 ccggggtgtc acggctaatt gttccataca atgtcactat ctttgtttca ggaataatca
    14161 attttcctgc aaatgtcttc ttgatataaa
//
LOCUS       DAJVPP010000096        14187 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVPP010000096 DAJVPP010000000
VERSION     DAJVPP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.096"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            34..435
                     /locus_tag="OQM34_003107"
     CDS             34..435
                     /locus_tag="OQM34_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1453454.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OQM34_003108"
     CDS             447..818
                     /locus_tag="OQM34_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1453455.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="OQM34_003109"
     CDS             805..1395
                     /locus_tag="OQM34_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1453456.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1793
                     /locus_tag="OQM34_003110"
     CDS             1392..1793
                     /locus_tag="OQM34_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1453457.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1804..2544
                     /locus_tag="OQM34_003111"
     CDS             1804..2544
                     /locus_tag="OQM34_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1453458.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2603..2989
                     /locus_tag="OQM34_003112"
     CDS             2603..2989
                     /locus_tag="OQM34_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1453459.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2998..3327
                     /locus_tag="OQM34_003113"
     CDS             2998..3327
                     /locus_tag="OQM34_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS1453460.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3299..6340
                     /locus_tag="OQM34_003114"
     CDS             3299..6340
                     /locus_tag="OQM34_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1453461.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            6340..6669
                     /locus_tag="OQM34_003115"
     CDS             6340..6669
                     /locus_tag="OQM34_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1453462.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6669..7367
                     /locus_tag="OQM34_003116"
     CDS             6669..7367
                     /locus_tag="OQM34_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1453463.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            7372..8115
                     /locus_tag="OQM34_003117"
     CDS             7372..8115
                     /locus_tag="OQM34_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1453464.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            8052..8654
                     /locus_tag="OQM34_003118"
     CDS             8052..8654
                     /locus_tag="OQM34_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1453465.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            8715..12194
                     /locus_tag="OQM34_003119"
     CDS             8715..12194
                     /locus_tag="OQM34_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1453466.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            12262..12861
                     /locus_tag="OQM34_003120"
     CDS             12262..12861
                     /locus_tag="OQM34_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1453467.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            12925..14187
                     /locus_tag="OQM34_003121"
                     /pseudo
     CDS             12925..14187
                     /locus_tag="OQM34_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
BASE COUNT         3357 a         3365 c         4502 g         2963 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgaccgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccga cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcagg
     1501 aactgcctgc cgtggcggtg tggctgagtg atgctcagta caccggaaaa atgacggatg
     1561 aagatgactg gcaggctgtt ctgcatattg ccgtcttcat ccgggcacag gcaccggatt
     1621 cagagctgga tatgtggatg gagagcacca ttttcccggc cctgaatgat gtaccggcac
     1681 tttccggact catcgacacc ctgaacccac tcggttttaa ctatcaacgt gataatgaga
     1741 tggctacctg ggcgatggcg gaaatcacgt accagatcac gtacacgaat taaggaggtg
     1801 gtaatgacca caccaaatcc actggcaaag acgaaaggtg cggggacgac gttctggatg
     1861 tataccggca acggcgatgc gtttgcgaac cctttgtcgg acactgactg gctgcgtctt
     1921 gcgatggtga aggatctgca acctggcgaa atgaccgctg atgcagaaga tgacacttat
     1981 ctcgatgatg aagatgcaga ctggaaaacg acaacccagg ggcagaaatc cgttggtgat
     2041 acttcggcga cgctggcctg gcgtccgggt gacagcgggc agaaaaaact ggttcagttg
     2101 ttcgactccg gtgaagtctg cgcgtttcgt atcaaatatc ccaacggcac tgttgatgtt
     2161 ttccgcggct ggctgagctc actgggtaaa accattgcct caaaagacgt gatgacccgc
     2221 actgtgaaaa tcagcggtgt ggggcgtccg tatctggcag aggaaggcac tgaaaccgtg
     2281 ggtgttaccg gactgacggt ggcaccggca tctgtcagtg tcaaagcggg agcaaccacc
     2341 acactgacct ttacagtaaa acctgacggg gccagtgaca aagcgatcag tgtgcattcg
     2401 tcagatcctc agactgcttc ggtgaccctg agcgggcttg tggccacggt gaaaggcgtg
     2461 aagcagggca gtgtcagcat cgtgggcatg acctctgacg gagagtttgt ggcagtggct
     2521 gcggtgaccg tcagcgcacc ataacaggac gatactcatc attgccccgg ttatccgggg
     2581 ctttttgcat ccggagaaca tgatgtttct gaaacaggat acgtttaatt atgaaaaaca
     2641 gtccgtggtg ctcagtgagc tgtccgggct gcagagaatt gaatatctga cgtttgttca
     2701 gcagcgaacg gcaaagtttg atgcacagga gggagaactg ccggaggctg aacgacagat
     2761 tgcttttctg cgtatgggaa tggatatcaa tgcctggctg gtttcccgct cactgtggaa
     2821 tgctgagcag tctcaggatg ttgagacgct ttgcgcatcc attatgacaa catggtcgta
     2881 tgatgcgctg ggcgcggggg cggagagggt tctgtcgctg agcggtatgg ggaccattga
     2941 gaatgccggg gatgatgatc atgaggcgct gacgccggaa aagtcctgac gcgggaaatg
     3001 cagtttgtca tgcggcttgc ccgggagttc cggcgggcag actggcggcg gatgctgtcg
     3061 gatatgtcgg ccactgagct tggtgagtgg ggcgattatt tccggatgca gagcttcagt
     3121 gatgtgtgga tggatgcgca gtttgcctcg ctgaaggcat tgatcgtgag aatggtgtcc
     3181 ggcagcagcg atgctgcggt ggctgatttc agccttttac cggaagagaa cgggataccg
     3241 gagcgaacgg acgaagaact gatgcatctt ggggaaggta tttccggagg tgtgcgttat
     3301 ggaccagata gccaacctgg tcattgattt ggggattgat gcggcagagt ttaaaaatga
     3361 aattccccgt atcaaaaacc ttctgaatgg tgcagccagc gatgcagaac ggtcttctgc
     3421 ccgtatgcag cgttttatgg agcgtcagac tcaggcggcc cggcagacaa tgcaggcggc
     3481 ttcttcggct gcaacagcag catcagccca tgcgcagacg gtggagaaga atgcacgggc
     3541 tcatgaacgc atggcccgtg aggtggaaca aacccgtctg cgcgtggatg ccctgaatca
     3601 gaaaatgcgc gaggaacagg cgcaggccag ggcactggcg gaggcgcagg ataaagcggc
     3661 tgccgccttt tatcgccaga ttgacagtgt gaaacaggcc ggtgcggggc ttcaggaatt
     3721 acagcgtatt cagcagcaga tccgacaggc cagaaacagt ggcggggttg gtcagcagga
     3781 ttatctggcg ctgatttcgg agatcacggc gaaaacccgt gccctgacgc aggcagagga
     3841 acaggccacc cggcagaaag cggcgtttat ccgccagctt aaagagcagg caacccgcca
     3901 gaatctgtcg tcttctgagt tgcttcgcgc cagggcggct cagctggggg taagcagtgc
     3961 tgcagaagtg tatatccgca aaatggagcg ggcaggaaaa gccacacatt cgctggggct
     4021 gaaaagtgcg gcagcccggc gggagctggg ggtgttaatc agtcagatgg cgcgcggcaa
     4081 ttttggtgcg ctgaggggat ccgggataac gctggctaac cgtgccggat ggacaggcgc
     4141 actgatgtcg ccgaaaggca tgatgactgg cggcgttatt ggcggacttg tcgcggcggt
     4201 cctgggtctg ggtaaagcct ggcatgacgg ccggaaggag ggcgagaaat ttaaccgtca
     4261 gctggcgctg acgggacatt atgccggtgt cactgtcggg cagttgtgga aactcagccg
     4321 ggccatatcc gggaatggta tcacgcaaca tgcggcagcc ggtgcgctgg cacaggtggt
     4381 gggcagtggg gcatttcatg gaaacgatat tggtatggtg gcgaaagctg ccgcacagat
     4441 ggagcgatcg gttggccagt cggtcagcga taccataaat cagtttaagc ggctgaagga
     4501 tgatcctgta aatgccgcga aggcgctgga caatgcgctg cattttttga atgccactca
     4561 gcttgagcag atacgcgtcc ttggggagca ggggcggtcc agtgatgcgg cccggatcgc
     4621 catgtctgcg ctggcagagg aaaccggtaa acgcacatct gatattgata ataatctcaa
     4681 tgcgctgggt agtacgctgc aaaccttgtc tgactggtgg aagcagttct gggatgcggc
     4741 catgaatatt ggtcgtgaag actcgctgga tgcgcagatt gatgcgttac aggaaaaaat
     4801 tcagcgcgcg aaaaaatatc cgtggacaaa cgcctccaca caggtggagt acgatcagca
     4861 gcgtcttaac gatcttcagg agaaaaaacg ccggaaggat ttgcaggatg caaaagcgca
     4921 ggcagaacgg aattaccagg agcaacagaa acgccggaat gctgaaaatg ccgcgctgaa
     4981 ccggatgaat gaaacggaag ctgcacgaca tcagcgggaa attgcgcgta ttaatgccat
     5041 gcagtacgcc gaccaggctg tcagggatgc ggcgatacag cgtgaaaatg aacgttacga
     5101 aaaagccatt aagaaaaata cacgggcaac ccgtaatgat gaggccaccc ggttattgct
     5161 gcagtacagt cagcagcagg cacaggtgga agggcagatt gctgctgcca gacagtcagc
     5221 aggcattgcc actgaaagga tgactgaagc gcataaacag cttctggccc tgcagcagcg
     5281 catcagcgac ctggacggga aaaagctgac ggcagatgaa aagagtgtgc tggcccgtaa
     5341 gaatgagctg attcaggcgc tgacgctgct ggatgtgaaa cagcaggagc tgcagaaaca
     5401 gacagcgctt aacgacctga gaaaaaaaac ggttcagctg accagccagc tggcagacaa
     5461 agaacgtgca ctgcgtgagc agcacaatct ggatattgcc actgcaggta tgggggataa
     5521 gcagcggcag cgctaccagg cacagttgcg catccggcag gaataccggc aacagttgca
     5581 acagcttgag aatgacagtc gccagaaagg cacttacggg acggaggact accggagggc
     5641 tgaggaggtg ctgaagggga gcctgaagcg acaactgaat gaaaacaaac gctactggca
     5701 ggaactggaa gtggcgcagg gcgactggaa aaacggtgcc atgcgggcgt ttcagaattt
     5761 tacggcggat gcggataatg cggcgggaac ggcagaacag atgtttacag tggcattcag
     5821 cagtgccggt aatgcactgg cgacattctg taccaccggt aagctgaatt ttaaatcctt
     5881 cacctcttcc ctgttgtcag atatggccag aattatggca cagatggcca tgatgcaggc
     5941 ggtaaagggc gtcggttctt tattcggctt cacgactaat gctgatggcg gtgtttacca
     6001 gtctgctgat ttgagtcgct acagtggcac ggtggttaac cgtccgacgt tttttgcttt
     6061 tgcaaaaggc gcgggtgtga tgggggaagc gggacctgaa gccattctgc cactgcgtcg
     6121 cggtgctgat ggtaagctgg gggttgtggc ggatactggt ggttcaggca tggtgatgtt
     6181 tgccccgcag tacaacattg agatcaataa cgacggcacg aacgggcaga taggtccggc
     6241 tgccctgaag gtggtttatg acctcgggaa aaaagcggcc gcggacttta tgcaacagca
     6301 ggcccgtgat ggtggccggt taagtggagc ataccggtaa tggagacgtt tcactggaaa
     6361 gtgcgcccgg atatgaatgt ggtatcagag ccgaaagtgg tgacagtgaa gctgggcgat
     6421 ggttatgaac agcgtcgtgc ggcgggactg aataaccagt tgtcgactta cagcgtgacg
     6481 atacgtgttc gtaaaggtga acacccatct ttaaaagcct ttctggaacg gcacggtggc
     6541 gtccgcgcat ttcagtggac gccaccttat gactggaaac tgatcagggt ggtttgtcgt
     6601 aaatggtcgg caagcgtggg ggcgttatgg gtgactgtaa cggccgattt tgaacaggtg
     6661 gtgaactgat gcaggatatc cggcaggaaa cactgaatga atgcacccgt gcggagcagt
     6721 cagcccgggt ggagctctgg gaaatcgatc tgacagaggt cggtggtgag cgttattttt
     6781 tctgtaatga gcagaacgaa aaaggtgagc cggtcacctg gcaggggcgg cagtatcagg
     6841 cataccccat tcaggggtcg gggtttgaac tgaacggcag gggctgtgct gcccgtccga
     6901 cactgacggt ctctaacctg cacggcatgg tcaccgggat ggcggaagat ctgcagagtc
     6961 tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg ttttctggat gcggtgaact
     7021 tcgtcaacgg aaacagcgac gccgatccgg aacaggaggt gatcagccgc tggcgcatcg
     7081 agcagtgcag cgaactgagc gcggtcagtg cctcttttgt gctggccaca ccaacggaga
     7141 cggatggcgc ggttttcccg gggcgtatca tgctggcgaa tacctgtatg tggacttacc
     7201 gttctgatga gtgtggttac acgggcaggg cagtggctga cgagttcgac aaaccaacaa
     7261 cggatatccg gaaggacaaa tgcagcaagt gtatgcgcgg gtgtgagttg cgcaacaata
     7321 ccggtaattt cggcggtttc ctttccatca ataaactttc tcagtaaatc catgacacag
     7381 acagaatcag cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc gtgtggcttc
     7441 gtggtgagaa cgccggaggg agacaggtat cttcccagcg agaatatctc cggtgagccg
     7501 gaggaacggt tccggatggc tccggaggac tggctgcggg cacaaatgca gggtgagatt
     7561 gtggcactgg tccacagtca tcccggtggt ctgccctggc tgagtgaggc tgaccggcgg
     7621 ctgcaggtgc agagtgattt gccgtggtgg ctggtctgcc ggggggcgat tcacaagttc
     7681 cgctgtgtgc cgcatctcac cgggcggcgc tttgagcacg gggtgacgga ctgttacacg
     7741 ctgttccggg atgcttatca tctggcgggg attgagatgc cggattttca tcgcggggat
     7801 gactggtggc gtcacggtca gaatctctat ctggataatc tggaggccac agggctgtat
     7861 caggtgccgt tgtcatcagc acaaccgggc gatgtgctgc tgtgctgttt tggttcatcg
     7921 gtgccgaatc atgccgccat ttactgcggc gatggtgagc tgctgcacca tattcctgaa
     7981 caactgagta aacgagagag gtataccgac aaatggcagc gacgcacaca ctccctctgg
     8041 cgtcaccggg aatggcacgc atctgccttt acggggattt gcaacgattt ggccgccgca
     8101 tcgaccttcg tgtgaaaacg ggggctgaag ccatccgcgc actggccaca cagctcccgg
     8161 cgtttcgtca gaaactgagt gacggctggt atcaggtgcg cattgccggg cgtgatgcag
     8221 gtgaaaccga attgtctgcc cgtcttaatg agccgctggc aaatggtgca gtgatccaca
     8281 tagtaccgcg tctggtggga gctaaaagtg gcggtgtgtt tcaggcggtg ctgggggcag
     8341 ctgttatggc ggttgctata tggatgccgg gggtaggaat tatggcgagt aatctgctgt
     8401 tttctctcgg tgccagtatg acgcttggcg gtgttgcaca gatgctggca ccgaaagcca
     8461 gaactccccg tacacagaca acggataacg gcaaacagaa cacctgtttc tcctcactgg
     8521 ataacatggt tgcccagggc aatgttctgc ctgttctgta cggtgaaatg cgcgtggggt
     8581 cgcgggtggt atctcaggag atcagcacgg cagacgaagg ggatggtggt caggttgtgg
     8641 tgattggtcg ctgatgcaaa atgtttcatg tgaaaccgcc tgcgggcggt tttgtcgttt
     8701 atggagcgtg aggaatgggt aaaggcagca gtaaggggca taccccgcgc gaagcgaagg
     8761 acaacctgaa atccacgcag ttgctgagtg tgattgatgc catcagcgaa gggccggttg
     8821 acggtccggt ggatggatta aaaagcgtgc tgctgaacgg tacgccggtc ctggatagcg
     8881 aggggaagac aaacttttcc ggtgttacgg tggtgttccg cgccggcgag caggagcaga
     8941 caccgccgga ggggtttgaa tcttccggct cagagactgt gctgggtacg gaagtgaaat
     9001 acgacacgcc gatcacccgg accatcacgt cggcaaacat tgatcgtctg cgcctgacct
     9061 tcggtgtgca ggcactggtg gaaaccacct caaaggggga ccggaatccg tcggaagtcc
     9121 gcctgctggt tcagatacag cgtaacggtg gctgggtgac ggaaaaagac atcaccatta
     9181 agggcaaaac cacttcgcag tatctggcct cggtggtggt gggtaacctg ccgccgcgcc
     9241 cgtttaatat ccggatgcgc aggatgacac cggacagcac cacagaccag ctgcagaaca
     9301 aaacgctctg gtcgtcgtac accgaaatca tcgatgtgaa acagtgctac ccgaacacgg
     9361 cgctggtcgg cgtgcaggtg gattcagagc agttcggtaa ccagcaggtg agtcgcaatt
     9421 atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag acgcggcaat
     9481 acagcggtat ctgggacgga acgcttaagc cagcatacag caacaacatg gcctggtgtc
     9541 tgtgggacat gctcactcat ccgcgctacg gcatggggaa acgtcttggt gcggcagatg
     9601 tggacaaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg ccggacggat
     9661 ttggcggcac ggagccgcgc atcacctgta acgcttacct gaccacacag cgtaaggcgt
     9721 gggatgttct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg aacgggcaga
     9781 cgctgacgtt cgtgcaggac cgaccgtcgg ataaagtgtg gacctataac cgcagtaatg
     9841 tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg aaggaccgcc
     9901 ataatgtcgt tgaggtgaac tggattgatc cggacaacgg tcatgagacg gcgacagagc
     9961 ttgtggagga cacgcaggcc attgtccgtt acggtcgtaa cgtcacgaag atggatgcct
    10021 ttggctgtac cagccgggga caggcgcacc gcgccgggct gtggctgatt aaaacggaac
    10081 tgctggaaac gcagaccgtg gacttcagcg tcggcgcaga agggcttcgc catgtaccgg
    10141 gtgatgtcat tgaaatctgc gatgatgatt atgccggtat cagcaccggt ggtcgcgtac
    10201 tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg ctgccgtcct
    10261 ccggtaccac gctgataagc ctggttgacg gaaatggcaa tccggtcagc gtggaggttc
    10321 agtccgtcac cgacggcgtg aaggtgaaag tgagccgggt tcctgacggc gttgcagaat
    10381 acagcgtgtg ggggctgaag ctgccgacgc tgcgccagcg cctgttccgc tgcgtgagta
    10441 tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg ccggaaaaag
    10501 aggccatcgt ggataacggg gcacactttg acggtgaccg gcgcggcacg gtgaatggtg
    10561 tcacgccgcc agcggtgcag catctgaccg ccgaagtcac cgcagacagc ggggaatatc
    10621 aggtgctggc gcgatgggac acgccgaagg tggtgaaggg tgtgagcttc ctgcttcgcc
    10681 tgaccgtggc agcggacgac ggcagtgagc ggctggtcag cacagccagg acgacggaaa
    10741 ccacataccg cttcaggcag ctggcgctgg ggaattacag tctgacagtc cgggcggtaa
    10801 atgcccgggg gcagcagggc gatccggcgt cggtatcgtt ccggattgcg gcaccggcag
    10861 cgcctgtcac tattgaactg ataccggggt attttcagat aacggtggtc ccgaaactgg
    10921 ctgtatatga cccgacggtg cagtttgagt tctggttttc ggaaaagcgg attgcggata
    10981 tcaggcaggt tgaaaccagc gcccgctatc ttggcacggc gctgtactgg atagccgcca
    11041 gtatcaatat caggccgggc catgattatt atttttacgt tcgcagtgtg aacaccgttg
    11101 gcaaatcggc atttgtggag gccgtcggtc gggcgagcga tgatgcggaa ggttatctga
    11161 gtttttataa agggttgatc aataaaacgc atctcggcaa ggagttgtgg acgcagattg
    11221 ataacggtca gcttgcgccg gacctgactg aaatcaggac gtccattacg aatgtcagca
    11281 atgaaatcac gcaaaccgtc aataaaaaac tggaaaatca gagtgctgca atccagcaga
    11341 tacagaaagt tcaggttgat acaaataata acctgaacag catgtgggct gtgaagctgc
    11401 aacagatgaa ggacggacgc ctttatattg cgggtatcgg tgccggtatt gagaatacgc
    11461 cagcaggaat gcagagtcag gtgctgctgg cggcagacag gattgcgatg attaatcctg
    11521 cgaatggcaa cacaaagccg atgtttgttg gtcagggcga tcagatattc atgaacgacg
    11581 tgttcctgaa acgcctgacg gctccgacca ttaccagcgg cggtaatcct ccggcatttt
    11641 ccctgacacc tggcggacgg ctgacggcga aaaatgccga tatcagcggt aacgtgaacg
    11701 cgaactccgg gacgctcaac aacgtcacga ttaataagaa ctgtcgggtt ctgggaaaat
    11761 tgtccgcgaa ccagattgaa ggcgatctcg ttaaaacagt gggcaaacct ttcccacggg
    11821 actcccgggc accggagagg tggccatcag ggaccattac cgtcagggtt tatgacgatc
    11881 agccgtttga tcggcaaatt gttattcccg cggtggcgtt tcgcggtgct aaacatgagc
    11941 ggaagaataa caatatttat tcgtcatgcc gcctgatagt gaagaaaaac ggtgctgaaa
    12001 tttataaccg aacgaccctg gataatacgc tgatatatac gggtgttatt gatatgcctg
    12061 ccggtcacgg tcacatgacg ctggagtttt ctgtatcggc atggctggta aatggctggt
    12121 atcccacagc aagtatcagc gatttgctgg ttgttgtgat gaagaaatcc acagcaggta
    12181 tcagtatcag ctgaatttta taacccagat acgggcacca gaaatggtgc cttttttatt
    12241 gcagaaaagc gagaggtaat tatgcgtaaa ctttatgctg ccattttgtc cgcagccatc
    12301 tgtctggctg tatccggtgc gcctgcatgg gcgtctgaac atcagtccac gctgagcgcg
    12361 gggtatcttc atgcctcgac gaacgctccc ggcagtgatg atctgaacgg gattaacgtg
    12421 aaataccgtt atgagtttac ggacacgctg gggctggtga cgtcattcag ctatgccaac
    12481 gctgaagatg agcaaaaaac gcattacagt gatacccgct ggcatgagga ttccgtgcgt
    12541 aaccgctggt tcagcgtgat ggcggggctg tctgtgcgcg tgaatgaatg gttcagcgcg
    12601 tatgcgatgg cgggcgtggc ttacagccgt gtgtcgacct tctccgggga ttatctccgc
    12661 gtaactgaca acaaggggaa aacgcacgat gtgctgaccg ggagtgatga caaccgccac
    12721 agcaacacgt ctctggcgtg gggggctggc gtgcagttta acccgaccga atccgtggcc
    12781 attgaccttg cttatgaagg ttccggcagt ggcgactggc gcactgacgg tttcatcgtg
    12841 ggtgtcggtt ataagttctg attagccagg taacacagtg ttatgacagc ccgctggttc
    12901 aggcgggctt tttgtggggt gaatatggca gtaaagattt caggtgtact gaaagatggg
    12961 gcgggtaaac ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac cagtccgacc
    13021 gttgtggcac acgttgttgc cacttgcgtg acggacaata acggtgctta tgtgattgag
    13081 gctgagccgg ggtattacga ggttgcgctt cactgtaacg gctggcagcc aacccgtgtc
    13141 ggggatattg atgtggcacc gactgatgca ccggggacac tgaacgcgtt tctgaatgca
    13201 ccaaaggatg gtgatttacg tccggaggtg atgaagcgct ttgaggaaat ggtggcgcag
    13261 gcgcagcaga gtgccggggc tgcagccgga aacgcacagc agacggcgca ggatgtggcg
    13321 gcagccgcaa cggcccgtga tgatgcacaa cgttttgcgg agaaagcccg acaggatgca
    13381 accgtcacag ctgaggacag aaaggccact gcggaagatg tgacaagcac aggagcaaat
    13441 gcagccgcag ccggacagag cgcacaggat gccgcaggtt atgcccgcgc agcagaacag
    13501 gccaaaaatg acattgatgc tgcgctgacc ggcactctga aaatggctaa ccatctgtca
    13561 gaaatcgcag cagcaggcga aaaggcacaa cagaagtccc gggataatct ggggctgaaa
    13621 agtgcggcca cgatggaagc acagagcgac atttacgacc ggacaaaagg ccgtctggcg
    13681 atacccggcg cattcggctt tgggtgtgct tttctgcctg aagatgttat ccgttttgac
    13741 actaagagtg atttcctggc ctgggtaagg aatgcgctgc caggtgaata ttccgttgct
    13801 ggcccctacg gcatcatcat acccgacaca cggtttgaag gggtgctcag catccggtgg
    13861 actgatgcac gccctgagac aacagaaccg cggtacagag ccaaatccct tactttttac
    13921 ggcattaacg gccccattta tcacacccgc taccgctact ggcccatatc cagactgact
    13981 gggtgaaaat aaatataacc acagaagata ttatttacag aatcgtggcg agctctgtcc
    14041 gcaacagatg gggagaccct gacattggcg ggctgattat tgctgcgtac cagggagaag
    14101 ctgacggtga taaagtcatc agacttgtca gggggcagtc atacagaggc tcacgactgg
    14161 gaccggtggg gatttcagtg cccagta
//
LOCUS       DAJVPP010000097        14130 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVPP010000097 DAJVPP010000000
VERSION     DAJVPP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.097"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..353)
                     /locus_tag="OQM34_003122"
     CDS             complement(<1..353)
                     /locus_tag="OQM34_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCS1453468.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASH"
     gene            complement(552..636)
                     /locus_tag="OQM34_003123"
     tRNA            complement(552..636)
                     /locus_tag="OQM34_003123"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(600..602),aa:Leu,seq:caa)
     gene            832..1851
                     /gene="ahr"
                     /locus_tag="OQM34_003124"
     CDS             832..1851
                     /gene="ahr"
                     /locus_tag="OQM34_003124"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1453469.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            1980..3482
                     /locus_tag="OQM34_003125"
     CDS             1980..3482
                     /locus_tag="OQM34_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1453470.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(3601..4683)
                     /gene="lptG"
                     /locus_tag="OQM34_003126"
     CDS             complement(3601..4683)
                     /gene="lptG"
                     /locus_tag="OQM34_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1453471.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(4683..5783)
                     /gene="lptF"
                     /locus_tag="OQM34_003127"
     CDS             complement(4683..5783)
                     /gene="lptF"
                     /locus_tag="OQM34_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1453472.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6050..7561
                     /gene="pepA"
                     /locus_tag="OQM34_003128"
     CDS             6050..7561
                     /gene="pepA"
                     /locus_tag="OQM34_003128"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1453473.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            7695..8138
                     /gene="holC"
                     /locus_tag="OQM34_003129"
     CDS             7695..8138
                     /gene="holC"
                     /locus_tag="OQM34_003129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1453474.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8138..10993
                     /gene="valS"
                     /locus_tag="OQM34_003130"
     CDS             8138..10993
                     /gene="valS"
                     /locus_tag="OQM34_003130"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1453475.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11047..12243)
                     /locus_tag="OQM34_003131"
     CDS             complement(11047..12243)
                     /locus_tag="OQM34_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCS1453476.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            12436..12939
                     /locus_tag="OQM34_003132"
     CDS             12436..12939
                     /locus_tag="OQM34_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1453477.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(12985..13401)
                     /gene="rraB"
                     /locus_tag="OQM34_003133"
     CDS             complement(12985..13401)
                     /gene="rraB"
                     /locus_tag="OQM34_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1453478.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            13563..>14130
                     /locus_tag="OQM34_003134"
     CDS             13563..>14130
                     /locus_tag="OQM34_003134"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HCS1453479.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAP"
BASE COUNT         3640 a         3596 c         3658 g         3236 t
ORIGIN      
        1 ctatgcgatg ccgaccactt ctgattgctg gcgtgccagt ctctggcgac tacttcaaac
       61 gttatcgcct cttgttcctg ttctacctta acagctttct tgttttcact gggatcgaca
      121 ccattagcta aaagcttacg agcctcatcc cggcgtgccc tggcatccgc caacgacact
      181 tcggggtatt tccccaatgc cagcatcttc tctttgccgc caaagcgata acgtagccgc
      241 cagtatttgg agccgttagg gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta
      301 taggcttttg cttcaggctt agccgaacga accttcacat cactcagagc catgatgagt
      361 atcctttcaa gggttctgtg tgggtacaaa cattatcgaa ccgggaaata cccgcagttg
      421 tacccgcatc agtaagttga tgtagattga atcaggttga cttaggttga gtgaaaaagc
      481 gaggaaagcc ttgcggatac tggattttag gcacaaaaaa agacgtccgt tgacgtctat
      541 tgatgttccg atggtgccga aggccggact cgaaccggca cgtatttcta cggttgattt
      601 tgaatcaact gcgtctaccg atttcgccac ttcggcactg aagaggtatg cggaaaacgt
      661 tgtggattat acctgtcgca cgccaccatg caagcgccag cttgcaccca cctcgctaag
      721 tgctgaaatt ttcagcatta cactcagcga tatgtaacct ttgtcacact ccaggcaccc
      781 cgccctgcca tgctctacac ttcccaaaca acaccagaga aggccccaaa aatgtcgatg
      841 ataaaaagct atgccgcaaa agaagcgggc ggcgaactgg aagtttatga gtacgatccc
      901 ggtgagctga agccacaaga tgttgaagtg caggtggatt actgcgggat ttgtcattcc
      961 gatctgtcga tgatcgataa cgaatgggga ttttcacaat atccgctggt tgccgggcat
     1021 gaggtgattg ggcgcgtagt ggcactcggg agcgccgcgc aggataaagg tttgcaggtc
     1081 ggtcagcgtg tcgggattgg ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt
     1141 agcggtaatc agattaactg cgagcaaggt gcggtgccga caattatgaa tcgcggtggc
     1201 tttgccgaga agttgcgtgc ggactggcaa tgggtgattc cactgccaga aaatattgat
     1261 atcgagtccg ccgggccgct gttgtgcggc ggtatcacgg tctttaaacc actgttgatg
     1321 caccatatca ctgctaccag ccgcgttggg gtaattggta ttggcgggct ggggcatatc
     1381 gctataaaac ttctgcacgc aatgggatgc gaggtgacgg cctttagttc taatccggcg
     1441 aaagagcagg aagtactggc gatgggtgcc gataaagtgg tgaatagccg cgatccgcag
     1501 gcactgaaag ccctgtcggg gcagtttgat ctcattatca atactgtgaa cgtcagcctc
     1561 gactggcagc cttattttga ggcgctgacc tatggcggta atttccatac ggtcggtgcg
     1621 gttctcacgc cgctgtctgt tccggccttt acgttaattg cgggcgaccg cagcatctct
     1681 ggttctgcta ccggcacgcc ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc
     1741 aaggttgcgc cgacaaccga actgttcccg atgtcgaaaa ttaacgacgc cattcagcat
     1801 gtacgcgatg gtaaagcgcg ctaccgagta gtcctgaaag ccgacttctg atccctttgc
     1861 cgggtgctgt cgcccggcac ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt
     1921 agcggtgaag agtgcacagc cgttgcctat actccaggca ggaaactgga ggaaatctca
     1981 tgagcgaacc cctgttaatt gcccgcacgc cggacacaga actgttttta ctgccgggaa
     2041 tggctaaccg tcacgggctg attactggcg caacggggac gggtaaaacc gttacgctgc
     2101 aaaaactggc agagtcattg tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag
     2161 gcgatctgac cggtatcgcg caggcaggaa cggcgtcgga aaaactgctc acaaggctta
     2221 aaaatatcgg cgtcaatgac tggcaaccgc atgccaatcc ggtggtggtg tgggatatct
     2281 ttggcgagaa aggccacacg gtgcgggcga cggtttcgga tctggggccg ctgttgctgg
     2341 cgcgactgtt gaatctcaac gatgtgcaat ctggcgtgct gaatattatc ttccgcattg
     2401 cggacgatca gggattgttg ctgctcgact ttaaagattt gcgggcgatt acccagtaca
     2461 tcggcgataa cgccaaatcc ttccagaatc agtacggaaa tatcagtagc gcatcggttg
     2521 gtgccatcca gcgcggatta ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg
     2581 agccgatgct ggatatcaaa gactggatgc gcaccgatgc caacggtaaa ggcgttatca
     2641 atatcctcag cgccgagaaa ctttatcaga tgccgaaact gtacgccgcc agcctgctgt
     2701 ggatgctttc agagttgtat gaacaattgc cggaagcggg cgatctggag aaaccaaaac
     2761 tggtgttttt cttcgacgag gcacatctgc tgtttaacga tgcaccgcag gtactgctgg
     2821 ataagattga gcaggtgata aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt
     2881 cgcaaaaccc gtctgatatt ccggataatg tgttcgtcca gctcggtaat cgtgttcagc
     2941 acgctttgcg ggcttttacg ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca
     3001 tgcgggccaa tccggcattt gataccgaaa aggcaatcca ggaactgggg accggcgagg
     3061 cgttaatctc gtttctcgat gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga
     3121 tcgcaccttg ttcgcgaatg gggccggtga cggaagatga gcgtaatggc ctgattaatc
     3181 actccccggt gtatggcaaa tatgaagatg aggtggaccg cgagtccgcc tatgagatgc
     3241 tgcaaaaagg ctttcaggcc agtaccgagc agcaaaataa tccccccgtg aaagggaaag
     3301 aggtggcggt ggatgacggt attcttggtg gattgaagga tattttgttt ggcactaccg
     3361 gaccacgcgg cgggaagaaa gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc
     3421 aggtgacgaa tcagattgta cgcgggatgt tggggagttt gctggggggg agaaaaagat
     3481 aagtcagacg tgatctgtgc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg
     3541 ttcgaactcg tatttgactt tgtcaacaat aataaaggga gctttcgctc ccttgttaga
     3601 ttacgatttt ctcattaaca gccacaggct gattaagaag aagctggcac ttggcaacag
     3661 tgcgccgatg atcggcggga tgccgtaaac caacgtcagc gggccgaaga tctggtccag
     3721 tacgtagaag acaaaaccga agctgatacc tgtgaccaca cgcacgccca tcggtacgct
     3781 acgcagtggg ccaaagataa acgacagcgc catcagcatc atcaccgcca cggatagcgg
     3841 ctggaagatt ttgctccaca tgttgagctg ataacgtccg gcatcctgac cgctcgactt
     3901 cagatacttc acgtagttgt gcaaaccact gatggagagt gcatccgggt ccagcgccac
     3961 cacgcccagt ttgtctggcg tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc
     4021 ggtaatctgt ttcggattgg tcagatcaga ttcatcaacc tgcgacagac gccagacttt
     4081 atgttccggg tcaaacttcg cggtagcggc ataacgtacg gattgcagac gacgattctc
     4141 gttaaaggca taaatactga tgccacctaa cacctcgtca cctttaaccc gctcaatgta
     4201 gacgaagttg ttgccatctt tcgcccataa accttgctgg gtagagagca acgagccgcc
     4261 gtacatcgcc tgcgcacggt agttacgcgc catctgttcg ccctgcggcg cgacccattc
     4321 gccaatcgcc atcgtcagca agaccagtgg aatggcggtt ttcatcaccg acagcgctac
     4381 ctgcatacgg gtaaaaccag aagcctgcat caccaccagt tcgctgcgct gcgccagcat
     4441 cccaagacca agcaacgccc caagaagagc cgccatcggg aagaagatct gcacatcttt
     4501 cggcacgctc agcaaggtat acattcctgc gcctaacgcg tcgtaactcc cctgcccggc
     4561 ttttttcagc tgatcgacaa atttgataat gcccgacagc gacaccagca tgaacagcgt
     4621 catcatgatg gtggtgaaaa tagttttacc gatatagcgg tcaagtacgc caaaaggttg
     4681 catcacaccg ctcctttacg cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc
     4741 caaaggttga gaacaatcgc caaagccaga taaatcaggt taacggtcca catccacagt
     4801 gtcgggtcca gcttaccttt accgccgttc gatttcaggg aggtctggat caggaagaaa
     4861 agtagataca gcagcatggc tggcagcatc gacagtacgc gtccctgacg cgggttaacc
     4921 acgctcaacg gtacaaccat aagtgccatc ataaacacgg tgaataccaa cgtgatacgc
     4981 cagttcagtt ctgcgcgagc acgatcggtg tcagtgttcc acaatgtgcg catgtccatc
     5041 tggtcggtat cgttcgggtc gagcgccacc gcctggtgac caatgatcgc ctgataatcc
     5101 tggaaatccg taatgcggaa atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg
     5161 ttgagagtga cgacctggga gccgtcgcgc agctgggtta aatgtccgga atcggccacc
     5221 accacagaag gacgtgcatt accttttggt cgaatttgtg cgaggaacac atctttgaaa
     5281 tcgctgccgt caacgctttc gatgaacagc accgagctgc cattagtcgc ttgctggaat
     5341 tgcccttgcg ccagcgccgc catgccaggg ttcgctttcg cttctgctaa cacttcatcc
     5401 tgatgacgcg atgaccacgg tcccgcccac atcacgttaa ccgccgcgac gattgccgtg
     5461 aataccgcaa ggatcattgc cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc
     5521 attaccgtaa tttcactttc ggtatacagt ttgcccagcg tcatcagcag cccgaggaac
     5581 aggcttaatg gcaggataag ctgcgccatt tccggcacgc ccaacccgag aagggagagc
     5641 accagattcg ccggaatatc gccgtcaacc gctgcgccga ggatcctcac taacttttga
     5701 cagaagaaga tcaaaagcaa gatgaagagt atcgccagct ggcttttgag cgtctcccgc
     5761 accagatatc ttatgattat cactttaaat acgcccgtaa aaactcgtct tttgcaggat
     5821 tttagcttgt ttcatggctt aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta
     5881 agataattat actcaacgga ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa
     5941 taacgttaag attaacacga agtcatcgca acagcggaca tgagttacga aagcttgcaa
     6001 ttctatctgt agccaccgcc gttgtcttta agattcagga gcgtagtgca tggagtttag
     6061 tgtaaaaagc ggtagcccgg agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga
     6121 accacgtcgc ctttctccga ttgcagaaca gctcgataaa atcagcgatg ggtacatcag
     6181 cgccctgcta cgccggggcg aactggaagg aaaaccgggg cagacattgt tgctgcacca
     6241 tgttccgaat gtactttccg agcgaattct ccttattggt tgcggcaaag aacgtgagct
     6301 ggatgagcgt cagtacaagc aggttattca gaaaaccatt aatacgctga atgatactgg
     6361 ctcgatggaa gcggtctgct ttctgactga actgcacgtt aaaggccgta acaactactg
     6421 gaaagtgcgt caggctgtcg agacagcaaa agagacgctc tacagtttcg atcagttgaa
     6481 aacgaataag agcgaaccgc gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg
     6541 ccgtgaactg accagcggtg agcgcgcgat ccagcacggt ctggcgattg ccgccgggat
     6601 aaaagcagcg aaagatctcg gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc
     6661 ctctcaggcg cgccagctgg ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg
     6721 cgaacagcag atgaaagagc tgggaatgca ctcttatctg gcggtcggtc agggttcaca
     6781 aaacgaatcg ctgatgtcgg tgattgagta caaaggcaac gcgtcggaag atgcacgccc
     6841 aatcgtgctg gtgggtaaag gtttaacctt cgactccggc ggtatctcca tcaagccttc
     6901 agaaggcatg gatgagatga agtacgatat gtgcggcgcg gcggcagttt acggcgtgat
     6961 gcgtatggtc gcggagctgc aactgccgat taacgttatt ggcgtgctgg caggctgtga
     7021 aaacatgcct ggcgggcgtg cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca
     7081 aaccgttgaa gtgctgaaca ccgatgccga aggccgcctg gtactgtgcg acgtgttaac
     7141 ttacgttgaa cgttttgagc cggaagcagt gattgacgtg gcgacgctga ccggtgcctg
     7201 cgtgatcgcg ctgggtcatc atattactgg tctgatggcg aaccataatc cgctggccca
     7261 tgaactgatt gccgcgtctg aacaatccgg tgaccgcgca tggcgcttac cgctgggtga
     7321 cgagtatcag gaacagctgg agtccaattt tgccgatatg gcgaacattg gcggtcgtcc
     7381 tggtggggcg attaccgcag gttgcttcct gtcacgcttt acccgtaagt ataactgggc
     7441 gcacctggat attgcaggaa ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg
     7501 tccggtagcg ttgctggcac agttcctgtt aaaccgcgct gggtttaacg gcgaagagta
     7561 agtttgcgtt ttaccctcac cctaaccctc tccctaaggg agagggaacc gatcgagcgc
     7621 aatagaaatt cattgctcat ctttcagggt gaggaatgca gcaatattta aatccaccac
     7681 aagaagcccc atatatgaaa aacgcaacgt tctaccttct ggacaatgac accaccgtcg
     7741 atggcttaag cgccgttgag caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg
     7801 gtaagcgcgt actcatcgcc tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc
     7861 tgtgggcgcg tccggcagaa agctttgttc cgcataattt agcgggagaa ggaccgcgcg
     7921 gcagtgcgcc ggtggagatc gcctggccgc aaaaacgcag cagcagcccg cgcgatatat
     7981 tgattagtct gcgaacaagc tttgcagatt ttgccaccgc tttcacagaa gtggtagact
     8041 tcgttcctta tgaagattct ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg
     8101 tggctggttt caacctgaat acggcaacct ggaaataatg gaaaagacat acaacccaca
     8161 agatatcgaa cagccgcttt acgagcactg ggaaaagcag ggctacttta agcctaatgg
     8221 cgatgaaagc caggaaagtt tctgcatcat gatcccgccg ccgaacgtca ccggcagttt
     8281 gcatatgggt cacgccttcc agcaaaccat catggatacc atgatccgct atcagcgcat
     8341 gcagggcaaa aacaccctgt ggcaggtcgg tactgaccac gccgggatcg ctacccagat
     8401 ggtcgttgag cgcaagattg ccgcagaaga aggtaaaacc cgtcacgact acggccgcga
     8461 agctttcatc gacaaaatct gggaatggaa agcggaatct ggcggcacca ttacccgtca
     8521 gatgcgccgt ctcggcaact ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg
     8581 cctgtccaat gcggtgaaag aagttttcgt tcgtctgtat aaagaagacc tgatttaccg
     8641 tggcaagcgc ctggtaaact gggacccgaa actgcgtacc gctatttctg acctggaagt
     8701 ggaaaaccgc gaatcgaaag gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc
     8761 gaaaaccgca gacggtaaag attatctggt ggttgcgacc actcgcccgg aaaccctgct
     8821 gggcgatacc ggtgtagctg ttaacccgga agatccgcgt tacaaagatc tgattggcaa
     8881 atatgtcatt ctgccgctgg ttaaccgtcg tattccgatc gttggcgacg aacacgccga
     8941 catggaaaaa ggcaccggct gcgtgaagat caccccggcg cacgacttta acgactatga
     9001 agtcggtaaa cgtcacgccc tgccgatgat caacatcctg acctttgacg gtgatatctg
     9061 tgaaagcgcc caggtgttcg ataccaaagg taacgaatct gacgtttatt ccagcgagat
     9121 cccggcagag ttccagaaac tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt
     9181 tgatgcactc ggcctgctgg aagaaattaa accgcacgac ctgaccgttc cttacggcga
     9241 ccgtggcggc gtggttatcg aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt
     9301 actggcgaaa ccggcagttg aagcggttga gaacggcgac attcagttcg taccgaagca
     9361 gtacgaaaac atgtacttct cctggatgcg cgatattcag gactggtgta tctctcgtca
     9421 gctgtggtgg ggtcaccgta tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg
     9481 ccgcaacgaa gaagaagtgc gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca
     9541 ggacgaagac gttctcgaca cctggttctc ttctgcactg tggaccttct ctacccttgg
     9601 ctggccggaa aataccgacg ccctgcgtca gttccaccca accagcgtga tggtatctgg
     9661 tttcgacatc atcttcttct ggattgcccg catgatcatg atgaccatgc acttcatcaa
     9721 agatgaaaat ggcaaaccgc aggtgccgtt ccacactgtt tacatgaccg gtctgattcg
     9781 tgacgacgaa ggtcagaaga tgtccaaatc caagggtaac gttatcgacc cgctggatat
     9841 ggttgacggt atttcgctgc cagaactgct ggaaaaacgc accggcaata tgatgcagcc
     9901 gcagctggcg gacaaaatcc gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc
     9961 gcacggcacc gatgccctgc gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat
    10021 caactgggat atgaagcgtc tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc
    10081 cagccgcttt gtgctgatga acacagaagg tcaggattgc ggcttcaacg gcggcgaaat
    10141 gacgctatcg ctggcggacc gctggattct ggcggagttc aaccagacta tcaaagcgta
    10201 ccgcgaagcc ctggacagct tccgcttcga tatcgccgca ggcattttgt atgagttcac
    10261 ctggaaccag ttctgcgact ggtatctgga gctgaccaag ccggtaatga acggtggaac
    10321 agaagccgaa ctgcgcggta ctcgccatac gctggtgact gtactggaag gtctgctgcg
    10381 cctcgcgcat ccgatcattc cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact
    10441 ttgcggtatc actgccgaca ccatcatgct gcagccgttc ccgcagtatg atgcatctca
    10501 ggttgatgaa gccgcactgg ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg
    10561 taacatccgt gcagaaatga acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg
    10621 ttgcagcgcg gatgcagaac gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc
    10681 gcgtctggaa agcatcaccg tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa
    10741 gatcatcgac ggcgcagagc tgctgatccc gatggctggc ctcatcaaca aagaagatga
    10801 gctggcgcgc ctggcgaaag aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa
    10861 caagctggca aatgaaggct ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg
    10921 tgagaagctg gaaggttatg cagaagcgaa agccaagctg attgaacagc aggctgttat
    10981 cgccgcactg taatcaacac agtgatgaaa acgaaggccg gagcatgctc cggccttttt
    11041 tatctcttac agaaatggta aagaaggcat aactccccga gaaatactgg ctaagaagcc
    11101 tttatctggt tgttcttctt tattgattaa aggaaggtca tccaaatcgc ccagcaaata
    11161 cgtatttttt gcctgccagt caatcatcca tattttcagc agtgggtaag ccagaccacc
    11221 cgtaatcccg gatatcacca ccaacgcaca catgcgataa agcataccgt ggtacgttaa
    11281 agttgagcga aaacgaatac gcccatcatt cagtgacagg ttgctcataa aatgatttcg
    11341 caacgacacc gttaaataac tggtgctaac agcaatccca aaataataaa ttaactgcgc
    11401 gattatcatt ttacgttgca tttccataaa ttgctgtaaa ttctcaatat catcatttgc
    11461 atacccagaa ctatcatact catttaatat ttgatcgaag ataatataac cagcaacagc
    11521 gagaaaaggt aataaagcta aaaatgcgag gatggcatat tttatacaat acgcagtatc
    11581 taatttcacc ttgaaacgat gtataccgaa actggtattg ctccagagaa aactcattac
    11641 caggctatat aaagtaccat taaaaatacc aatggaaaca ataccaacta ttgccatcag
    11701 aaccacagat ataataacac tacttgaact attggcatgt agcatctttg tcgagataaa
    11761 gaaaacagtc cccatcccaa tggccattaa aatcggcaag aaaaaggtta cccaccagaa
    11821 ccctttcaaa gagcaattaa aactaaatct tacgccgttg agactggtca ttaaggcctg
    11881 ataacgcagt ccttttgctg ccataaatat aagcaaaacc aacagtaaca aagtcaaaac
    11941 acagccaaca agcggcatat ctgctgacac tgtcattaag attgcgaaat agaaaaaaac
    12001 aaaaacaaga caactaacaa aaacattccc accggtaatt ccataagaaa atcgttgtcc
    12061 gttaacttcc atattagcat aaagataacg cttacatttc attaatgccc atggtaaata
    12121 gatacctagc gttataatcg ttaacaacac attcaccaca caaataagaa aatatctctc
    12181 acctgtacca tgaaaaacaa aagaatggga cggaacatcc atttcattaa taacttgagc
    12241 cataaaaatc ccctgaatta aaaaatccat tgatataatt tatagataca gacagtaaaa
    12301 tgttctgcca cgttacaata ctcttatcag taataggctg gcaaaaactt tattgcttta
    12361 taagacgctt gcgcacgtaa gaagagagtg gtattaaaca aatgtataat tactttattt
    12421 ttattgggtt aaactatgaa taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg
    12481 ctgcaggata atcctgctat cgccagcgtc atccgtcagg tttacgcaga gtatggtctt
    12541 accgccgata aaggctacac cgttgccgat ccgaatctcg acgaactgta tcaagtgtac
    12601 agccagcctg gccatgctta ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc
    12661 gggattgcgc cattaacagg tagtgaagcg gacatttgcg aactgcagaa aatgtacttt
    12721 cttcccgcta ttcgcggtaa agggctagcg aaaaaactgg ccttaatggc gatggagcag
    12781 gcacgagaga tgggcttcaa acgctgctat ctggaaacga ccgctttttt aaaggaagcc
    12841 attgcgcttt atgagcattt gggctttgag catatcgact atgcgcttgg ctgcacgggc
    12901 catgtcgatt gtgaagtgcg gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg
    12961 ctatcgggca ttttaatcaa ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa
    13021 atcttcatcg tcgccatctt cgccgttagg atcttcaaag taagtgcccc aaccgtcgta
    13081 ctcaacgtca aatttctccg ccagcgtcat cagttgttca acctgggcat cgatcagatc
    13141 ggcattcaac gcgcactcgc tgaggatgtc gcagcaaatc acgatatcac catcttcaac
    13201 ttccagctct tctggatcgg tcacttcgta accgagttta aacgcttcaa ctgccgcttt
    13261 ttccagagtt tccagatcgt ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg
    13321 atcgctgcca tcttccagta attcttcaat aattaaacgt gtttcttcac gctgttcttc
    13381 cagttgttcc gggtttgcca tggctcattc ctcttaatgt cctgccgata ctcttattgt
    13441 cacacaccac tgacattgcc tccacctttg tgacaaagat ttatgcttta gacttgcaaa
    13501 tgaataataa tacacataaa ttgaatttta attcattgag gcgttagcca caggagggat
    13561 ctatgtccgg gttttatcat aagcatttcc tgaaattact cgatttcacg ccagctgaac
    13621 tcaacagcct gctgcagtta gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag
    13681 aagccaaact cactggtaaa aacatcgcgc tcatcttcga aaaagactcg actcgtaccc
    13741 gatgctcttt cgaagttgcc gcatatgacc agggggctcg cgttacttat cttggcccaa
    13801 gcggtagtca gattggtcat aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca
    13861 tgtatgacgg tattcagtat cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat
    13921 acgctggcgt gccggtatgg aacggcctga ccaatgagtt tcaccccacg cagttgctgg
    13981 cggatctcct caccatgcag gagcatttac ccggtaaagc attcaacgaa atgacgctgg
    14041 tctatgcagg tgacgcacgt aacaacatgg gcaattcgat gctcgaagct gcggcgctta
    14101 ccggtctgga tttgcgtctg gtcgcgccac
//
LOCUS       DAJVPP010000098        14072 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVPP010000098 DAJVPP010000000
VERSION     DAJVPP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.098"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..906)
                     /locus_tag="OQM34_003135"
     CDS             complement(<1..906)
                     /locus_tag="OQM34_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1453480.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQ"
     gene            complement(949..2323)
                     /locus_tag="OQM34_003136"
                     /pseudo
     CDS             complement(949..2323)
                     /locus_tag="OQM34_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2369..4405)
                     /gene="yihQ"
                     /locus_tag="OQM34_003137"
     CDS             complement(2369..4405)
                     /gene="yihQ"
                     /locus_tag="OQM34_003137"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCS1453481.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            complement(4604..5506)
                     /locus_tag="OQM34_003138"
     CDS             complement(4604..5506)
                     /locus_tag="OQM34_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCS1453482.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(5644..6885)
                     /gene="yihS"
                     /locus_tag="OQM34_003139"
     CDS             complement(5644..6885)
                     /gene="yihS"
                     /locus_tag="OQM34_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCS1453483.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPALLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(6902..7780)
                     /gene="yihT"
                     /locus_tag="OQM34_003140"
     CDS             complement(6902..7780)
                     /gene="yihT"
                     /locus_tag="OQM34_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCS1453484.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(7804..8624)
                     /locus_tag="OQM34_003141"
                     /pseudo
     CDS             complement(7804..8624)
                     /locus_tag="OQM34_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            8792..9688
                     /gene="yihV"
                     /locus_tag="OQM34_003142"
     CDS             8792..9688
                     /gene="yihV"
                     /locus_tag="OQM34_003142"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCS1453485.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            9722..10507
                     /locus_tag="OQM34_003143"
     CDS             9722..10507
                     /locus_tag="OQM34_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1453486.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            10606..11205
                     /gene="yihX"
                     /locus_tag="OQM34_003144"
     CDS             10606..11205
                     /gene="yihX"
                     /locus_tag="OQM34_003144"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1453487.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            11199..12071
                     /locus_tag="OQM34_003145"
     CDS             11199..12071
                     /locus_tag="OQM34_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCS1453488.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            12068..12505
                     /gene="dtd"
                     /locus_tag="OQM34_003146"
     CDS             12068..12505
                     /gene="dtd"
                     /locus_tag="OQM34_003146"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1453489.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            12550..13491
                     /gene="fabY"
                     /locus_tag="OQM34_003147"
                     /gene_synonym="yiiD"
     CDS             12550..13491
                     /gene="fabY"
                     /locus_tag="OQM34_003147"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1453490.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            13907..>14072
                     /locus_tag="OQM34_003148"
     CDS             13907..>14072
                     /locus_tag="OQM34_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453491.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNEL"
BASE COUNT         3441 a         3518 c         3677 g         3436 t
ORIGIN      
        1 ctgtttttta ccaaaacatt ttacagttac tggcaccagt aatacgccga cgaggatgca
       61 tcccatactg aagaatccca tccacgacaa taaattaata tcattcagca catactgggt
      121 gtaatagacc tgaatcgcca gtttgatatt aaatgccgcc agagtacaca ggttagcaat
      181 gcataaaacc agcaatggtg gattacgaaa tatcgcgcaa aaagatttga gaatactggc
      241 tttatgtccg gttggcgctg tgtccacata atgctctttg acgcctctgt agcacagcat
      301 cataaaaata aagccgccaa tggagaacat aagtgccgca caggcataac cgacggttga
      361 gtcagaaaaa agcgactgca acggaataaa cgccacggta caaatcaata gccctatggt
      421 tgcaccaccc tggcggtaag ccgcgagctg cgcgcgctca ttcgggtttt tggtaattgc
      481 cgggatcatc gcaccatacg agcagttcat cagactgtat gaaagtccaa acatcataaa
      541 cagcgtggtg gcaatcgtcg ttttaaccgg caaacaaaag gtggtggcga taaactggag
      601 cgttgcaatt aacgccgccg gaactgctgc atataaaata aagggacgaa atttgccttt
      661 aggcccaata tttttccgcg agtcgagtaa aaatccggtg agcatatcgg taaacgcggt
      721 aaaaaacttc gcgaccagaa agataatccc gccatagtaa gcaggcatcc ctaattcatc
      781 ggtataaagt ttgaggagat acagagtacc gatgcagagc attaaattcg aaccgacgtc
      841 gcccatacca taggcgattt tttcccgcag attcagtttt aatgtcagtg gattatggtc
      901 agacataatc actccttata acgccccgca gggcgtcgtt tattattatt acgccgtgcg
      961 tttacgggct tctatctctt ccacaatgcg gacatacatc ttctcgttca ggctgtagaa
     1021 gcaacccatc gccacaatgg tgactaccgc cagtgcgctt gggtagatga agatcaactg
     1081 gcgtaaccct tcaatggtgt ggtcagcctg tgcgacgttc ggcacatagc caatttgcgt
     1141 cagcatccag ccggggaaga aaccagccag cgcctgagac actttgcgaa agaaggtgaa
     1201 gccggtgtag accgtgcctt ccgaacgcac gccggtgcgc cactcgccgt actcgacggt
     1261 gtcggaaacc agcgcccagt tcaggcgctg agccgaagaa cgccaggcag gagaacgcca
     1321 caaagctgac cgagccgccg ccgaagaaat agttgagcag atcgcccagc acccaaatca
     1381 gcaggccgcc gatatagacc ttcttcttac caaaacgtct gactgcgcca ggcatcagga
     1441 acacgccgat gaaaatacag cccatgctga aaaatcccat atacgacaac aggatgggat
     1501 cgttaagtac gtactgggtg taatagacct gaatggcgag cttgacgtta aacgccccta
     1561 aggtgcagag gttggcaata cacagaatga acagtgggcg gttaccggca attgcgcgga
     1621 aagattgcaa caggccaggc ttttgcgccg gattggttgg ttgggtttcg acataacgct
     1681 ctttcacgcc tgagtagcag atccacataa acagcaggcc gaacagtgaa aacagcgtgg
     1741 cggcgaagat atagccaagt ttctgattac cttcgataag attcataact ggcacgaatc
     1801 ccaccgtgca cagcagcagg cccagcgtag cgccgccctg acgccatgcc gccagtgagg
     1861 cgcgttcgtt ggggtttttg gtgattgccg ggaccattgc gccgtaggag cagttcatca
     1921 tgctgaaaaa cagtccgtaa agcataaaca gaatagtggc catcaccgtt ttaccggtga
     1981 catcaaacgg tgtgccgaca aagttggcga tcgccagtaa ggtgaccggg aatgacgcat
     2041 acagaataaa aggacggaac ttaccttttg gaccgatctt gcgtcgggaa tccaacataa
     2101 tgccggtacc catatcggta aacgcagtaa agaattttga aatcaaaaag ataatgccgc
     2161 cataggtgcc tggcagcccc agaacgtcgg tataaaactt caaaagataa agcgtaccga
     2221 tatccagcag gatgttagag gccaggtcgc caataccgta agagagtttc tctttaaagg
     2281 gcaggcgtaa agttgctgga tcttccgttg tgatgtgact catcgatgtt ctccctttgc
     2341 ccatgagttt ccccacgggc ggatgtgatt agatgctgtt taacgacgcg aacagtgccg
     2401 cccattcgct atcggcgcga taaaagaccg gcggcttgcc gatgggcgca ttaacggtaa
     2461 cttccccgcc ccggaacgct tcacccgtcc aggcatgtac ccagttatcc tccggcagat
     2521 agagcgtcca gtcgctgcga ccttcttcgt gtaccggagc gaccagaatg tcgcggccta
     2581 acaggtactg atatttcagg gaataggtat gtgcatcgtc ttcgtaatgc aggaacagcg
     2641 ggcgcatgac cggcagaccg gacttcgcat tcagcgcgac ggcctctttc agataaggtt
     2701 tcagggtggt gaaaacggta gtcatacggg caaaatgggc gatagtttcc gcatcgccgt
     2761 caaactgcca gttgtcgcca ggacggttac cttcgtgggt gcgcatcatc ggcgtgaagg
     2821 cgctgaaatc gcaccagcgc agcagcagct ctttgctgcg cttcatctca aacagggtgg
     2881 tgtaaccgcc aatgtcgctg tgatgcagac catgtccggt cattgccagc gacagcgccg
     2941 ccgggactac ggacgccagg ccatcgtcga gactccagtc cacgttctgg tcgcccgccc
     3001 acatcatggt ggagtatttc tggctaccgg tagaaccggc gcgcataaag aagaggatct
     3061 cgccgagctt gcccgtttct tcaagggctt cgtagttaca cttcgcccac agcgcaggcc
     3121 aggcgttatg cataatttcg gcactgacgc cgttatgcag gtacgtgtcg gtgggcagat
     3181 actcgccgaa gtcagccatc cagccgccgc agccgagttc aatcatgttc tttttgatca
     3241 cttccttgaa ccaggcgtag gcttctggat tagtgagatc gacaacgccg ccgtaaaact
     3301 cgccaaactc caccagatag tcaccgccag aggcatcttt tgctagatag ccgtgttgtg
     3361 ccgcttcttc gcagagatct ttatcgctgg caacatacgg gttgatatag gccaggaact
     3421 gcacgccctc ctgataccac tgcttaatgc gtgaatccag ttgcgggtag ttttcgctgt
     3481 tccacttcca gttccacatc acgcgcttgc caaaagaggt tatacggatc ccagaccagt
     3541 cctgcgccca gatgccgtta accttcacgc ccgcgttacg catggtgtcc agtttcttct
     3601 ggcacacttc cgtcccgccc tgaatgccga gcgttacccc gtcataaatc cagtcgggca
     3661 gttctggctg gcgtcccagc agggcagtta atttttccag cagggagatg tatgtgtcag
     3721 cacattcaaa acgtagcgta gctttgtctt cccacagcgc cagttcatgg tattccgggg
     3781 cgctaaagtc gaagttcatg tagcaactgt tatcaacatg gcagtaatac ttctgcgtgc
     3841 tgacaaacgt aggctgtggg aagaaagtcc agtaatagtc gccgcccgca ttttctttgc
     3901 agtcggcctg ccaggtgaca taggtttgtt tgttgcgacc aacgccttgt tcactggtcc
     3961 atagcgggaa cggtttgccg cgcagatcga agtaggaaaa ctgttcgccg cagccgtaga
     4021 tatggtcctc tggttgagcg gcaaggcgca gccagatacg gttgtggtta aggttgtcgt
     4081 tttgtagttc cagcaataaa cggccctgat cgtcggcaga gatattcagc gtggcgctaa
     4141 tgtcagaacc acggctgaaa tgaattaacc aaccatccgg tgactggctg acgatggcgt
     4201 cggtaagcgc aattttctcc tgtagtttat ctttaatgct gaaattaccg cggaacatat
     4261 cgatatccgc tatacctgag ccaatccata aacaaggatt atctttgtta tgggttaaaa
     4321 taagacgttg ttgaaaatgt agggtaaaac tgtcgttatt ctgatgaaat tgaaaatcta
     4381 ataactgtgg acgtggcgta tccataacta atctccatag agttgagaac gagattgttg
     4441 ccttcctgtt attacaggct tatgtaaaat gattctcata aaactgatat ataaaaactt
     4501 tacagaagct gagctgtttt taacttaata aattgaagat acataatgtt gcctttagcg
     4561 agtgaaatgt tcttcaatga ttttgactgc taatcaatac aagttatttt ctctgtccgt
     4621 aaatattaaa aaacaggcgg tgcggttttc ctggttccag taataataag tcgatacctg
     4681 aattaaaggc gttaggcggg caactcatag gttcaactgc cagtccctga cgctgtaatt
     4741 tttcaccgct ataaacctgc acccagggtt gatcggaaca aaggcttacc gaaagcgcct
     4801 gctgtggatg ggtaatggtc atttcccaca aatcattagc ggttttaaag gtgtggtcga
     4861 tttttgtcgc ggctattttt ttcgcctgcg tgaaatttaa atccagctca tcaacgtggt
     4921 gaagcgttgt cggatttacg tgttcatcga cggcataaac ctggtttgcc ggaagctgga
     4981 acagatattc atcaacagat gttaagttgc aggttaaata aggatgaata cccacgccat
     5041 aaggcgctgc gacagtgccg atattctggc ttgcgatctc aacggaaaga cccgtgtgtg
     5101 cgttgagcga ataaacaact tgtgaagcca gcataaacgg atagccgtaa ctgggcggca
     5161 gaaaagctgt cagggtaacg ctggtggtgg ttaattcact aatttgccag tctcgccagg
     5221 caagcagccc gtgaatagcg gcttttgaac tatgttcatt aatgggtaac tgatattcct
     5281 gcccctgata acggtaacag ccgttggcga tccggttcgg ccacggaata agcactttac
     5341 ccagatgcgc cagcggcatc tcttcgggtt tgtgcgggat gactaaatga caaccctgaa
     5401 aggttaattc agccagacca gcaccgacgg tcacaatggt cgcgtgataa tcccccgcgg
     5461 caagtgaaac ggtctgtcca ctgcaatgca tattggtgat ttgcatacaa ggcaccttaa
     5521 ttaacgacat acagcctccc gtgaggctga tgtcaaaccc aaaaactgca ccggacggtc
     5581 ccctcgcccc tttggggaga gggttagggt gaggggaaaa cccgcaccct attttcagat
     5641 cagttatttc gcattaatat ccagcagacc cgctgcaacc gctggagcca tgccgggcgc
     5701 taacgggata cgcgggatca ccaggcaatg cagcaggtga taaatatcct gtttgccgtc
     5761 ccagactttg gtgatgacct tattgtccgc atccagctcc tgccaccagg aaccattttc
     5821 atagtccatc aggtacttaa tgcagtactc ccaccatgtt tgataccagg tttcatactg
     5881 gcgatcgcca gtgacggtgt agagcgcgta ggccgtaccc attgcttcga cgataggcca
     5941 acgtacacgt tcgcggacca ccggttttcc ttcccagtca acggtataaa caatcccgtc
     6001 cgctccatcg ggtgcccagg catcgcgcac ggtggcgtta aacagacctt tggcatcttc
     6061 tagcagcaat gctggtggtt gttcgcaacg ggcttccagg gcagcatgga tgtgcagcat
     6121 taaacggccc cattcgatcc agtggcctgg tgtacctccg aacgcgcgga agcgatgcgc
     6181 cgggttatct ttgttgtaat ccggcagcgg attccactgg gtatcgaagt gttcgttaac
     6241 gcgataatga ttatttctgg cgacgtcgtg gataatcacg gaagccacgc gaatcgcgcg
     6301 atccagccat tttttgtcgt gagtgacgtc ataaacaatc aagaaagctt ccaccgcgtg
     6361 catattggca ttgccgccgc ggtactcttc ggttttgctg aaggcttcgt cccaggactc
     6421 caggcacatc tgctcttctt cgctccagaa atatttctcg ataatctcaa tggtgtaatc
     6481 gagcagcttg cgcgcttccg ggtgacccgt tgtgacggcg ctggcggcac ccagcagagc
     6541 aaagaagtgt tgatagccct gtttggaggc atccaccacg ccctcgtcat tcacgcaggc
     6601 ataccagccg ccatattttt tatcacgcag tgcgccgttc atggctttaa taccgtgatc
     6661 aaccaacgcg taagcgccag gtcgacccat cgccgcagca acggaataaa cgtgcaacat
     6721 acgagctgtg atccacagat gggtgcccat ctcttctttg atttgccctt tattgcctaa
     6781 ccagccaaaa ccagtcggca ctacggaatt tttaccaaaa tcaaagatgc ggtcggtttc
     6841 ctgttccagc caacggttgt ggcttagggt gttaaaccat ttcattcctt ttatcctcat
     6901 cttagcggcg tttagccatc atttcgtcga cgatatcgcc aagttgttgt aatttcggtg
     6961 cgcaaacgtc acggagcatc agctcgttgt ctggtaaacc gacgaccgat gcccagactg
     7021 cacggcctgc caggaatccc gatgcgcccg ccgtcattgc cacgcgtacg gcacgcggga
     7081 acagtttttc gtcgacactg gaagagagga tcacccatgg catattgata tggtcattca
     7141 gacgttgtga agcacagaga agctcttgtt gcggaccttt gccataaagc ggcatttcaa
     7201 ctttgtagag gtcagcgcca ctgtcgccca gctctttggc ggcatcgatg atcgcttgtt
     7261 cgcgatcgaa tttatcgcca cgacgcggtg ggcggacgac tggctcaatg atgcttacca
     7321 gaccgtgtga gtggcacagt tcgttgaact ctttcaccat atccagacgt tgctgcgcat
     7381 cttcatcact acgccacagc accagcagtt ttaaggcttt gccaccgtct tgtttgatct
     7441 gtagcggatt gattttgcgg tcaataacca cgctatcgac cggaataccg ttgccaggaa
     7501 tgaactcatc ggcggcgaca atcatggcgc aacttttggc aatcgcgttt tgctcaacca
     7561 cctggcggta gcagaattgt tgatctacca gaatcgccga ggcataaggc gagagggcct
     7621 ttgcagcgtt aactttgaaa tcagttaaaa cgctatcggc taccggagcg ggtgccccag
     7681 ccgcggcaaa catcatgcgc atggcttcgc gctgatcgac cgccagcatg gcaaaaccgc
     7741 ccgatgcgcg cgtaatgtcg ttgatggtgt acttattcat tcatttattc ctttatccag
     7801 tcgttacatt tttactttgg cagtcatccc ggcactgaca cggacctgtt ccagaatggc
     7861 ggaccagtcc tggcgaccgt gacccgctgc gcgcgcctgg ctataaacct cccgtgaggc
     7921 ggcccccagc ggcattggca catgcagctg gttggcgaca tcaagggcga tgccaagatc
     7981 cttatgggca agatcgatca tgaaggcggg agaaagatct ccgctgagga ctttgtttgg
     8041 ccaggaagtg gtgaagtgac ctttaccggc ggcggtaccg ctcatcactt tgacggcaac
     8101 atcgaaggga agattcaggg cttcgcacaa aacggcggct tctgccgaaa gcgcattgag
     8161 cgcgatgctc atgtagttgt tgatgagctt aacgcggatc cccatgcccg gaccgccagc
     8221 gttgatcaac tcactgccca tcgccatcag gatcggtgtg gcacgttcaa cttgttcagc
     8281 ggtgccgcca gccagcagta acagagtacc ggtaatggca tttgcagcgg atggatggtg
     8341 gacatatcaa tgaccagcgc atcggtagat aagccttcgc aaacgccgtt ttcaccgaac
     8401 aacacgttgc gcaccagatc gccattcggc aacatggtaa tgataaattc ggcatcttta
     8461 gcggcctgcg ccgggttggc ggcgggagtc gcacctttgt ctaccagatg ccgcacagct
     8521 tcggcgttca catcaaagac gcgaagttga tgcccttgct gcaataaatt gctcgccatt
     8581 ggcgaaccca tttgtcctaa accgataaac gcgattgctg ccataaccct ctcctgaata
     8641 cagtgatgtc actttttgtc atttatgaca tgctttgctt gtctgttttt gatcgaattt
     8701 gtaatttatc gtcaaaaaat tgacagccgt cactttttaa acaattggtg aaattaaaat
     8761 gaacgcatcc caaaatgttt aaggaatgac catgattcgt gttgcttgtg taggtataac
     8821 cgtgatggat cgcatctatt acgtggaagg gttaccgacg gagagcggta aatacgtggc
     8881 gagaaattat acggaagttg gtggcgggcc agcggcgacg gcagcggttg cggcggcaag
     8941 gctgggggcg caggtcgatt ttattggtcg cgtaggtgat gacgacgcgg gcaacagcct
     9001 gctggcagag ctggaatcct ggggcgttaa cacccgttac accaaacggt ataaccaggc
     9061 gaaatcttcg caatccgcca tcatggtgga taccaaaggc gagcggataa tcattaacta
     9121 ccccagcccg gatctgctgc ctgacgcaga gtggttggag gaaattgatt tctctcagtg
     9181 ggatgttgtg ctggcagatg tacgctggca cgacggcgct aaaaaagcct tcaccctggc
     9241 ccgtcaggcg ggtgtgatga ccgttctgga cggggacatt acgccgcagg atatcagtga
     9301 gctggtggca ttaagcgatc acgcggcctt ttcagaaccg ggtctggcgc gcttaacggg
     9361 cgtgaaagag atggccagtg cgctaaaaca ggcacaaacg ctcacaaatg gtcatgtcta
     9421 tgtgacccag ggtagcgcag gctgcgactg gctggaaaat ggtgggcgtc agcatcaacc
     9481 ggccttcaaa gttgatgtgg tagataccac aggtgcgggt gatgtttttc acggcgcttt
     9541 ggcggtggcg ctggcaacaa gtggggattt agcggagtca gtccgcttcg ccagcggtgt
     9601 agcggcgtta aaatgcacac gtcccggtgg acgagccggg atccctgact gtgatcaaac
     9661 ccgatctttt ttgtcacttt ttgtataaaa tgccagggtg atggtttttc gaggaatttt
     9721 catgagcctt accgaactaa ccggtaaccc gcggcacgac caactcctca tgctgatcgc
     9781 cgagcgtggg tatatgaata ttgatgagct ggcaaatctg ctggatgtct ccacgcagac
     9841 ggtccgccgg gatattcgta aattaagcga gcaaggcctg attacgcgcc atcacggtgg
     9901 cgcgggtcgg gcttccagcg tcgttaatac ggcgttcgag cagcgtgagg tttcgcaaac
     9961 cgagaaaaaa aaagcgattg ccgaagcggt ggcagactat attcctgatg gttcaacaat
    10021 atttatcacc attggtacga ctgttgagca tgttgcccgg gcgttactta accataatca
    10081 tttgcggata atcaccaaca gcctgcgtgt ggcgcatatt ctttaccaca acccgcgctt
    10141 tgaagtgatg gtgcccggcg gtacgttgcg ctctcataat agcgggatca ttggcccttc
    10201 agcggcgtcc tttgtggctg attttcgtgc tgattatctg gtaacaagcg ttggggcgat
    10261 tgagagcgat ggcgcgttga tggagtttga tgtaaacgaa gctaacgtgg tgaaaacgat
    10321 gatggcgcac gcgagaaata ttctgctggt cgccgatcac actaagtatc atgcttcggc
    10381 ggcggttgaa attggtaacg tggcacaggt cactgcgctc tttaccgacg agctgccgcc
    10441 cgctgcgcta aaatcacgct tacaagacag ccaaattgaa atcattcttc cccaggaaga
    10501 cgcgtagatt tttgtgaccg ttaacctggc ttcatacctt gccacatagc caaacccatc
    10561 ctttcccgct acagttaatt tcttgtggcg cgaaaggagg caaaaatgct ctatatcttt
    10621 gatttaggta atgtgattgt cgatatcgac tttaaccgtg tgctgggagc ctggagcgat
    10681 ttaacgcgta ttccgctggc atcgcttaag aagagttttc atatggggga ggcgtttcat
    10741 cagcatgagc gtggggaaat tagcgacgaa gcgttcgcag aggcgctgtg tcatgagatg
    10801 gctctaccgc taagctacga gcagttctct cacggctggc aggcggtgtt tgttgcgctg
    10861 cgcccggaag tgatcgccat catgcataaa ctgcgtgagc aggggcatcg cgtggtggtg
    10921 ctttccaata ccaaccgcct gcataccacc ttctggccgg aagaataccc ggaaattcgt
    10981 gatgctgctg accatatcta tctgtcgcaa gatctgggga tgcgcaaacc tgaagcacga
    11041 atttaccagc atgttttgca ggcggaaggt ttttcaccca gcgatacggt ctttttcgac
    11101 gataacgccg ataatataga aggagctaat cagctaggca ttaccagtat tctggtgaaa
    11161 gataaaacca ccatcccgga ctatttcgcg aaggtgttat gctaaaaacc attcaggaca
    11221 aagccaggca ccgtacccgt ccactatggg cctggctaaa actactctgg caacgtattg
    11281 atgaggacaa catgacaacc ctggcaggta accttgccta tgtgtcgttg ctctcattag
    11341 tgccgctggt tgccgttgtt tttgcgcttt tcgccgcttt tcccatgttt tccgacgtca
    11401 gcattcagtt acgtcacttt atttttgcca actttctgcc tgctactggc gatgttattc
    11461 agcggtatat cgaacaattt gttgctaatt ccaacaagat gaccgccgtt ggggcgtgcg
    11521 ggctgatcgt caccgcgtta ttgttgatgt actccatcga tagcgcgttg aataccatct
    11581 ggcgcagtaa acgagcgcga ccaaaaattt actcgttcgc cgtgtactgg atgattttaa
    11641 cgctggggcc gctgctggca ggggccagtc tggcgatcag ttcctatttg ctctccctgc
    11701 gctgggcgag cgatctcaat actgtcatcg acaacgtgct gcgtattttt ccgttgctgt
    11761 tgtcgtggat ctccttctgg ttgctgtaca gcattgttcc taccatccgc gtacctaacc
    11821 gcgacgcgat tgtcggcgcg tttgtcgccg cactcctgtt cgaagcagga aagaaaggtt
    11881 tcgcgcttta tatcaccatg ttcccgtcat atcagctcat ttacggtgtg ctggcggtga
    11941 tccccattct ctttgtttgg gtctactgga catggtgtat cgtcttgctt ggcgcggaaa
    12001 ttactgtcac tctcggggaa taccgcaaac taaaacaagc agctgaacaa gaagaagacg
    12061 acgaaccatg attgcattaa ttcaacgcgt aacccgtgcc agcgtcaccg tggagggaga
    12121 agtgacgggc gaaattggcg cgggactttt ggtgttattg ggtgtcgaaa aggatgacga
    12181 cgaacagaaa gcaaaccgtc tgtgcgagcg tgtgctcggc taccgcatct ttagcgatgc
    12241 cgaaggcaag atgaatctca acgtgcaaca ggcgggcggc agtgtactgg tggtttccca
    12301 gtttaccctc gccgcagata ccgaacgggg gatgcgccca agtttctcca aaggcgcatc
    12361 accggatcgc gcagaggcgt tatatgacta tttcgtcgaa cgctgccgcc agcaagagat
    12421 gaacacgcaa acaggacgct tcgctgccga tatgcaggta tcgctggtca atgatggccc
    12481 cgtgacattc tggttgcagg tatgagccag cttccagggt tgtcacggga aacaagagag
    12541 agtatcgcta tgtatcacct tcaggttcca caaacagaag aagagttaga gcgttactat
    12601 cagtttcgct gggaaatgtt gcgtaagccc ctgcatcaac caaaaggttc ggaacgcgac
    12661 gcgtgggatg cgatggcgca tcaccagatg gtcgtcgacg agcagggtaa tctggtggcg
    12721 gtaggccgat tgtatattaa tgccgacaat gaagcgtcca ttcgctttat ggtcgttcac
    12781 ccggacgtgc aggacaaagg gttaggaacg ctgatggcga tgaccctgga gtcggtggcg
    12841 cgtcaggaag gcgttaagcg cgtgacctgt agcgcccgtg aagacgcggt ggagtttttc
    12901 gccaagctgg ggtttgttaa tcagggagaa atcaccacgc caaccaccac gccgattcgc
    12961 cattttttga tgattaagcc cgtcgccact ctggatgata ttttgcatcg cggcgactgg
    13021 tgcgcgcagc tgcaacaggc gtggtacgaa catatcccgc ttagtgaaaa aatgggcgtg
    13081 cgtattcaac aatataccgg gcaaaaattt atcaccacca tgccggaaac cggcaatcag
    13141 aatccgcacc atacgctgtt tgccgggagt ttattctcgc tggcgacgct caccggttgg
    13201 gggcttatct ggctgatgct gcgtgaacgc cacctcggcg gaacgattat tctggcggat
    13261 gcgcatatcc gctacagcaa accgattagc ggtaaacccc atgcggtagc cgaccttggt
    13321 gccttaagcg gcgatctcga ccgtctggcg cgcggacgaa aagcacgggt gcagatgcag
    13381 gtcgaaatct ttggcgacga gacgccgggt gcagtgtttg aaggcacgta tatcgttctg
    13441 cccgcgaagc catttggccc gtatgaagag ggcgggaacg aagaagagta gcttatggag
    13501 aggggcggtg ctgcctctct cattcattcc ggtcatttac atcagtccct gactattccg
    13561 gttatattga ataagaactt tataaatttt tattcttcgt tggcaccata cgtgtcttgc
    13621 tacatcactg ttcaggagca tttaaatatt atttgtagag ctgagtggca attattgcag
    13681 ataatgctta actcatcata ttagttcagg tttgagcgat ttttgatagt caaaaccccc
    13741 tctggtgcca tacttttttc ataaatattt ttaaaatcta tagctcacaa atattatgca
    13801 aagataatgg gctggcatac attgtcagta tcattcattt actgctctgt atgttatgaa
    13861 tcattatgtt gtatgtttgc tcttaaaccg tgggaaggag gctataatgg ttgccaatat
    13921 taatctcatt aaacaagaaa gttattctgt tgttaattta gaaaaacaac taatgagtaa
    13981 taaatcaccg aagcaaacgc tattaaatca attttcactt tgccttaaaa tggaaggtga
    14041 ggagcaactg tgcgattgtg ttaacgaact ga
//
LOCUS       DAJVPP010000099        14022 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVPP010000099 DAJVPP010000000
VERSION     DAJVPP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.099"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(337..582)
                     /locus_tag="OQM34_003149"
     CDS             complement(337..582)
                     /locus_tag="OQM34_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453492.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="OQM34_003150"
     tRNA            complement(1227..1302)
                     /locus_tag="OQM34_003150"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="OQM34_003151"
     CDS             complement(1408..2115)
                     /locus_tag="OQM34_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1453493.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="OQM34_003152"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="OQM34_003152"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1453494.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OQM34_003153"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OQM34_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1453495.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OQM34_003154"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OQM34_003154"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1453496.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="OQM34_003155"
     CDS             complement(7299..7574)
                     /locus_tag="OQM34_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1453497.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OQM34_003156"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OQM34_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1453498.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="OQM34_003157"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="OQM34_003157"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1453499.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="OQM34_003158"
     CDS             9534..9860
                     /locus_tag="OQM34_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1453500.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="OQM34_003159"
     CDS             10044..10763
                     /locus_tag="OQM34_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1453501.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="OQM34_003160"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="OQM34_003160"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1453502.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13064
                     /locus_tag="OQM34_003161"
     CDS             12102..13064
                     /locus_tag="OQM34_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS1453503.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13165..>14022)
                     /locus_tag="OQM34_003162"
     CDS             complement(13165..>14022)
                     /locus_tag="OQM34_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCS1453504.1"
                     /translation="NVSSCITGLLIPPSNVLIVFSLTAGGVSVASLFMAGYLPGILMG
                     LAVMIVCGIIAKRRGYPLSERATFAQACKAFLDALPSLLLVFIVMGGIFTATEASAIA
                     VVYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMIS
                     DALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIM
                     MVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLLFYAALLVALMMITYIPQISL
                     FIPQLLGLM"
BASE COUNT         3646 a         3358 c         3613 g         3405 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc ataccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctagaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatagcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcggtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccatca caatgaacac cagaagcaaa cttggtaagg catccagaaa
    13801 cgctttacag gcctgagcaa aggttgctct ttctgaaagc gggtagccac ggcgtttagc
    13861 aataatgccg cagactatca ttaccgcaag acccattaag atccctggca aatatcccgc
    13921 cataaacagc gatgctacag aaacgccgcc agctgtcagg gaaaacacaa ttaacacgtt
    13981 tgagggcggt atcagtaatc cagtaataca agatgagacg tt
//
LOCUS       DAJVPP010000100        13800 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVPP010000100 DAJVPP010000000
VERSION     DAJVPP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.100"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..818)
                     /gene="wcaD"
                     /locus_tag="OQM34_003163"
     CDS             complement(<1..818)
                     /gene="wcaD"
                     /locus_tag="OQM34_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS1453505.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGT"
     gene            complement(793..2010)
                     /gene="wcaC"
                     /locus_tag="OQM34_003164"
     CDS             complement(793..2010)
                     /gene="wcaC"
                     /locus_tag="OQM34_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS1453506.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTSGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2007..2495)
                     /gene="wcaB"
                     /locus_tag="OQM34_003165"
     CDS             complement(2007..2495)
                     /gene="wcaB"
                     /locus_tag="OQM34_003165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS1453507.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2498..3337)
                     /gene="wcaA"
                     /locus_tag="OQM34_003166"
     CDS             complement(2498..3337)
                     /gene="wcaA"
                     /locus_tag="OQM34_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCS1453508.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3430..5592)
                     /gene="wzc"
                     /locus_tag="OQM34_003167"
     CDS             complement(3430..5592)
                     /gene="wzc"
                     /locus_tag="OQM34_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS1453509.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5595..6038)
                     /gene="wzb"
                     /locus_tag="OQM34_003168"
     CDS             complement(5595..6038)
                     /gene="wzb"
                     /locus_tag="OQM34_003168"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS1453510.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6044..7183)
                     /gene="wza"
                     /locus_tag="OQM34_003169"
     CDS             complement(6044..7183)
                     /gene="wza"
                     /locus_tag="OQM34_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS1453511.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7492..7641
                     /locus_tag="OQM34_003170"
     CDS             7492..7641
                     /locus_tag="OQM34_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453512.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7842..9425
                     /locus_tag="OQM34_003171"
     CDS             7842..9425
                     /locus_tag="OQM34_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1453513.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9877..11730)
                     /gene="asmA"
                     /locus_tag="OQM34_003172"
     CDS             complement(9877..11730)
                     /gene="asmA"
                     /locus_tag="OQM34_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1453514.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11752..12333)
                     /gene="dcd"
                     /locus_tag="OQM34_003173"
     CDS             complement(11752..12333)
                     /gene="dcd"
                     /locus_tag="OQM34_003173"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1453515.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12425..13066)
                     /gene="udk"
                     /locus_tag="OQM34_003174"
     CDS             complement(12425..13066)
                     /gene="udk"
                     /locus_tag="OQM34_003174"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1453516.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13384..>13800
                     /locus_tag="OQM34_003175"
     CDS             13384..>13800
                     /locus_tag="OQM34_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCS1453517.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3196 a         3640 c         3492 g         3472 t
ORIGIN      
        1 gtacctaaat cgcccagacg ggtggaaata taaggaaacg cgattaccac accaacccgg
       61 aatacagcca gagaaatcaa tgctaacggt aattttttct taatcgcctc tttattcaga
      121 tattgaaatg cccactccag caaataaaac aggataaagg tcataacccc tgaaaacgat
      181 ccggataata ttatccctgc gagaatcagg gcatctgttt taggcgtttt gataccaaac
      241 tgtttgatgc tgagccaaat tgagattaat gccagagcga aaaatgccgg ttcgaaataa
      301 agtgctgtcg tgcgcttgcc gccgaattta atgaaattca gcacatagct gttgctgtaa
      361 atcagatatt tcgaaatcga ctccataata ctgctgccac cggtgaggat aatttgcgcc
      421 atctccaccg ccgccagcgc caccaccagt ccgaccacca gataaaagaa acgtaatatc
      481 ttacgatggt tgtgtggcga aattgtttta aaacgaatac tccacaccat gccaataatg
      541 atcacaatat agacaaacag catagttgaa gtgacgtatt taccggcatc caacgactga
      601 ccaaacaggt agttaaacgc cgtgagtccc gcgccaatcc ctaaggcaat catcaatttt
      661 ttaatgctga tgcgttctaa aaacagcagt aacaagacag gtaaaaaagt gacgatggtg
      721 atggggaaac tttcgccaag ctgggcgatt ttgacgttaa ccagcaggta gatcagtggc
      781 agcagcaggt agctacagat tctgatagaa gttgacatac tcctccagca tctgttgtcc
      841 actgtaggcg gcgcgactgc gttggctgaa ctcagccagc gtggtaccaa atatcgcctg
      901 cgcgatttcc ggtttgctta actgtaccag ttgcagcacc tcttcttcac tgacggtttt
      961 accaccggat ttttgcagca cttcccgcgc cgcatcgcta tgggtggcaa tcaccggcac
     1021 gccaatcgat agcgcctcac acaaaatcag cgggtagtta tcgactcgag aactgaacac
     1081 cagcgcatcc atctgattga gcgcgctcat caacttgcgc ttgtcagttt caaagccgtg
     1141 attaaccacg ttgccagcgg tgaacggcga gaacttacca aaggtatgca gttcgatttt
     1201 gtcgcccagc gccatcatct cacgcaccag ttgctggtta gttttgccgt cgtaacgcag
     1261 gtcatgcgcc accactgcga ttttcggctt gccctgagtt tcgcgcaccg gaggcagatc
     1321 tgccagaatc gcttcggttg ccatatcaat gccattattg ataatccggc aacgccctgg
     1381 cccgtacaga ctattgaaag cgtcagccac atgctggctg ggggaaataa actgacagcc
     1441 cagcgccagc atctcgcgga ataactggcg tttgcccgcc accagctggt gcgcgcgatc
     1501 aatcttcacc ggcggatagt tatttaaggt cgggcatttc tggcagcccg ttttccagcc
     1561 ttcgcaaccg tcggtaaagg cgcagcgtcc ggttacactc cagtggtcgt gcagcgtcca
     1621 gaccagggtg acatccggtt tatggttttt cactttttcg caaaagcgca ccacgctgtt
     1681 aagattcaac cagtagctgt gcagcacatg aaaatgcagg accaccggac ccgatgtgcg
     1741 agtaatggtg cgatataact cattgaaatt gccgaacaga tcgcgattaa acagacgaaa
     1801 caacgcaatg ttcgccatcg cggtcatccg cggcgtatgt ttgatgacct gcggatagtt
     1861 ctgatggctg acgctctctt tgccgccttt gccgtagccg tagacaaaat gtgacgccag
     1921 cccctgttgc agcgcacgct ggtggagatc taacgccaca cctgctgccc cgccttccgc
     1981 cagtcgcaca ttaaattgca gaatattcat ttaatcacct tcactcgcgc tttttctccc
     2041 accaccagcg cgttgtccgg gacagagtcg agcaccacgc tgcccgcacc cacggtgacg
     2101 ttgttaccaa gcgtgatatc accaagaata atgacgttgg caccgagttc gacgccgttg
     2161 ccaatgtgtg gacatgccat gttatcggca ccccgattgc cgatagtgac gccgtggcga
     2221 atggtgaaat catcccccgc caccacgttt ttattgatca ctacggcgta accgtgatgg
     2281 atagtaaagc ggcggccaat ggtcgcggcg gcctggattt cataaccgaa aaagcattcg
     2341 gtgataatgc gatacagcac cagcagcggg gccgcccaca gattgttgag gacgttcttt
     2401 ttgcgccaca ccgaacaaaa atgagcgacg cgataggcaa gtaccatgca gcacgggcgt
     2461 aaactccagc tgttggcgcg cagatcttcc agcatcttta gcgcccccga ataccatcag
     2521 ccagacgttt gccgttacgc accgacagca gcgtcaacaa ggtgcgccag gtcatacgtt
     2581 tattgcggat ctggtagagg gtaaacagct gatatttttt actggcgcgg tcgaatttgt
     2641 ctttgtgctt gcggtaaaag tggaagtatc cggagaattt tttcggtgac gaggtgatct
     2701 gcatttcgcc gtgattgata tgcaggatct gcgtcgcctc ttccactttc cacggttcgc
     2761 cgtactccac caccatccgc aggaatatgt cgtaatcctg tgctgctttc agttcggtat
     2821 cgaacaaaca ctctttgaaa cgccatgccc aggtaaagac ctgattgcca atgatattac
     2881 gtttatagaa caggcggcgt gagtacggcg atttgggata cagtggtaag ctcgccggtt
     2941 gggaataaac ttctccctgg caaacgtagt cgttagcgta caaaaaggcg tgtgtgacca
     3001 gttgctgttt atgggcgagg aagacgctca ggcggttggg cgtccattca tcatcatcat
     3061 cgatcccggt gatgtattcc ccttgcgcca gcataatcgc ctggttacgc accgcgcacg
     3121 ccccgctgtt aatgtcgttg tggatgtaag tgatacgcgg atcgttgagg gcagtgacgt
     3181 actgttgcag ttgttcccag gaggtggagc aatcatccac gatgatcatc tcccagttgc
     3241 tgtagtcctg gcgcaggacc gattttatcg cccgaatcgc cagttgttgg cggttccagg
     3301 ttggcatata gattgagatc agcggattgt ctttcatagg ttgttctccc ggcttatgag
     3361 ctggtttgct tgccggatgc ggcgtgagcg ccttatccgg cctacggggc ggtgcgaatg
     3421 caggcgtcgt tatttcgcat ccgacttata ttcgtattcg taatagccat aatcctgata
     3481 cgcgctggcg cggcggaaga tggagttcag aatcacccct ttcaccggaa taccgttttg
     3541 ctcaaagcgg ctcagactgg tttccacttc tttcaatgtg ttgaccgcat aacgcgccac
     3601 cattaacgtg gttccgacat gacgaccaac aattgccgca tcggtcactg ccagaatcgg
     3661 cggcgtatca atcaacacca ggtcatagtt tttactcgcc cagttcacca gttcggcaaa
     3721 gcgttcgctc attaacagtt cagaaggatt tggcggcacc tgaccgcgcg ggatcaggtc
     3781 aaatttggca atagaggtcg gtttagcagc ggtagtaata tcgccctgac caatcagaat
     3841 ctccgacagc ccattgacat tattggtgcc caacagctcg tgggtgtagc ctttgcgcat
     3901 atcgcagtcg atcaacaaca cgcgtttatt ggtctggctg atcaccgccg ccaggttggc
     3961 gcagacaaag gttttaccga ttgacgggct aaccccggtc atcatcaaca cattgttctg
     4021 cgcctgcatc atcgcgaagt gcagactggt acgcagacta cggatggctt caatcgccag
     4081 atcggttgga ttccccactg ccagtagctg gctctgttta tagcgtttaa cccctttgat
     4141 ggttttgacg ctatcgcgcg ctttctgcca ctccgacagc gggatgctgg catagacgct
     4201 gattccgtgt tcttccagca cttgcgggct ttcgatgccg cgattaaaca acgagcgcag
     4261 cagcacaccc acgatagaga gcatcaggcc aaggataatc gcgccgagga taatcagccc
     4321 tttcttcggt tttagcacgc caggctgagt gattgccggg tcaataatgc gcacatcgcc
     4381 gacggtgctg gcttcggtga ttttcagctc ctgctcttta ttcagcagtt gcatatagac
     4441 ctgctgacca gactcgacat cgcgggtcag acggacaatt tcctgctggt ttttcggcat
     4501 cgccgttacg cgaccgttaa gtttggcttt ctcttcttcc agcgcctgac gtttctccag
     4561 cagcgtgcgg tacgccgggt gaactttggt gtacagcttg gagatttccg cctctttaaa
     4621 ggtcagttcg ttcaactgcg cgtcgatgtt caccatcgaa tcgagcaccg ctttcgcttc
     4681 aagcggcaga tcaacagaat ctttatcctg acggaaggca ttcagtttgt tttcggcaac
     4741 atcgaggcgg ctacgtactt ccggtaactg ttgcgcgagg aaagcgaggc ttttcgacgc
     4801 ttccgccgat ttgcgctcaa tattttgttc ctgatagtta cgagcgatgc tgttaagaat
     4861 gtcgcggatc tgttcgcgat cttcaccggt ataagtcagg ctcagtacgc ctgcgtcttt
     4921 gccgttctcc gttaccgtca ggctgttttg cagttggttg atcatcccca acgtggagta
     4981 tttggtgacg gtaaactcac tgcccgggcg ggcgtgaatg gcttcaacca tcagcgtgac
     5041 gccttctttt ttcagcatct ggcccgcttg cccacgggcg ctaaagccgc catcactgct
     5101 cagggtgtag tttttgtcat ccagcacatt aagcgtaaac acctgatccg ccatctcttt
     5161 cgggcggtta aaggtagtca ctttcaccgt ctcgttctga cgccccatca ggcgatccca
     5221 gcccgccccg aaaatcggga acgtgttttt gctcaccgca atatcgaggt cgagatcgtc
     5281 caccgtttta ccaagcacca gacgcgaacg aatcaactgg atctcggcgt ccgatgcagg
     5341 cggtttgttg gctaatgccg agccgatatc ctgcaccagc gaattgccgc tgttttgctc
     5401 gatttgtacc agtgcgtcgg cactataaat cggcgtggcg aagaaagtgt aaaccacggc
     5461 acagagggca aatacgacgg tgatgccaat cacccaccag cgcgcttcaa tgacggtgcc
     5521 gaccaggcga ccaatatcga tttcatcact gcccgttacc ggagcggcat gttgttttac
     5581 tttttctgtc attcttatac ctgctctgcg ttcaatgcct gcgcccactg gcgggcagac
     5641 cgttcaagta atgtgtacac cgctgcaaac gtttcccggc ttttgcgata cggatcgggg
     5701 atttcacatt cgttatccca gtgaccaaac agcatcactt tgccgcgcat ctccggtgcc
     5761 atctcgcata agcgttcgat atggcgctta tccatggtca aaatcaggtc atagttgcga
     5821 cacaggcggc ggctgatttg tcgtgcacag tggccttcca gagacagttg atgttctgcg
     5881 gcgacgctga tagcggtagg atcagcgccc ttaccgacca gcgcgccgag tccagcggac
     5941 tccactttca gttccgggtg ataacgttgc agtaagcgtt ccgccatcgg ggaacggcaa
     6001 atattgccga cacagacaac taagatgttg ttaaacatga cgattaccag ttatgaatgt
     6061 cgctggctgt atccgtcatg taacggacac cgctaatagt tggcagcaac tgattgatca
     6121 gacggttcca gcgggaaacc ggggcggtgg tgacatacac cacgtcataa ggttgcaggc
     6181 ggaattctgt cgccatcacc agcgacgtcg catcggacat atccagctgg tagatattgg
     6241 caatcttgcc gttacgcccg ccctcgcctt tcagcggacg aatgacaaag atgccgctgg
     6301 cgttggaggt ggtcatgtcg ataccttcgg cattggccag agcttcagtc agggtcatgc
     6361 cgctaaagtc cattttcagg gtgctctgtt tcttcacttc acccatcaca aatactttca
     6421 gatcatcatt acgcggcacg tagagaatat cgccgggata aagcagtcgg ttctgattga
     6481 ggtcgccgtt ttgcatcagc gcctgtaaag aaatgcgttc ttcacgacca ttgtgtgtta
     6541 gcaccacgtt gcgccagtca gcggtgtcgg tcaggccacc tgcggcgttg atggcgtcga
     6601 gaatggtcag tggtacgttg gtgatcgcct gttgaccgga tttattcacc tgacctgaga
     6661 tataggcctt ttgtgagcgg aaggcggcga tattaacgtc cacctgcggg tcagcgatgt
     6721 acgtcgctaa gcgcccggta atatcactgc ggatttcagc gagcgttttc ccgactacgt
     6781 gaaccttgcc gatatacggg taaaacatag tgccgtcagg ctgtacccag ttgccggtgt
     6841 cgctggagct gcggtactga ccagctggcg tggtgagttc cgggtgatcc cagacggtga
     6901 cattaagaac gtcccccggc ccgacgcgat actggtaatt cgcgatctcg ctttccagcg
     6961 tcatattggg gcgcgctaca ttcgggcgtg ggcgtaattg gtcaatcagg cgcggggtca
     7021 gcggataaac attcaccatt ttgtcgagat cgaaatcagc gtcctgctgt ttgatgacgt
     7081 ctttgcccat cgtcgacata ttgctgcccg gaagtactgt gcaaccgctt atcaaggtta
     7141 ctgacaccaa taatggcatc aatttcattt tggatttcat cattgtttat ttatcacttt
     7201 ggcagagtaa ttatcctgtg cactattaat agcaatgtcg ccatgcacat ttaccttgca
     7261 gttaattgaa taaaaattta actggcatca gtcctaaaaa aattgatttc atccgcaggc
     7321 tattgacaga ataattcaga ctggtctttc aggcatccag acacgctacc gcccctggct
     7381 ttttagctac caatacactg attgagctta atttttcaaa ccctctctgc atacaatcgt
     7441 tgatgagaaa gggttattgc ggaaattaac ttccgaatat aaggtgacat tatggtaatt
     7501 gaatattggc tcgccaataa tgcaggagga agtgttacag ctaacggaat agcaggcaag
     7561 gtaacaattc ggcaattggc tatttttaag aattatatta agtatcattc aatatggttt
     7621 ttaggagttt ctttaggttg acaatattta atattgcgtc tccacatgcg atatttctta
     7681 aataatgttt tattattacc gcttttaatt cagggataat gtgaggttat taccctcaaa
     7741 taatatgaga taaatagtgc tgcaacattg catttttgcc ccgatttatc catgatcgaa
     7801 ttgtgacatt tgtcatacaa cgaataggtt ttgtacttac tatggaatgg attgccgatc
     7861 cgtctatctg ggccgggtta atcacgctga ttgtgatcga actggtcctc ggcattgata
     7921 acctggtctt tattgccatc ctcgccgaaa aactaccgcc gaagcagcgc gaccgcgcac
     7981 gggttaccgg gctgctgctg gcgatgttaa tgcgcctgtt actgctagcg tcaatctcct
     8041 ggctggtcac cctgactcaa ccgctgttca gcttccgctc gtttaccttt agcgcccgcg
     8101 acttaatcat gctgtttggt ggtttcttcc tgttgttcaa agccacgatg gagctgaacg
     8161 aacggctgga agggaaagac agcaataatc ccacacaacg caaaggtgcg aagttctggg
     8221 gcgtggtgac gcaaattgtg gtactggacg ccattttctc actcgactca gtgattaccg
     8281 ccgtcgggat ggtcgaccat ttactggtca tgatggccgc cgtggttatc gctatcagcc
     8341 tgatgttgat ggccagcaag ccgttaacgc aatttgttaa cagtcacccg accatcgtta
     8401 ttctctgctt aagcttcctg ttgatgattg gctttagcct ggtggcagaa ggtttcggct
     8461 tcgtcattcc gaaaggctac ctgtacgctg ccattggttt ctcggtgatg atcgaggcac
     8521 tcaatcagtt ggctatcttt aaccggcgac gttttctttc cgctaatcag acgctacgcc
     8581 agcggactac cgaagcggta atgcgcctgc ttagcgggca aaaagaagat gcggaactgg
     8641 acgccaaaac cgcgtccatg ctggtggatc atggaaacca gcagatcttt aatccgcagg
     8701 aacggcggat gattgagcgg gtacttaatc ttaaccagcg taccgtaagc agcattatga
     8761 cgtcgcgcca cgatattgag catatcgatc tcaacgcgcc ggaagaagaa atccgccagt
     8821 tgctggagcg aaatcagcat acgcggctgg tggttaccga cggtgatgac gcagaagatt
     8881 tgctcggtgt tgttcacgtt atcgacctgc tacaacagtc actgcgcggc gaaccgctca
     8941 acctgcgggt gttgattcgc cagccgctgg tgttcccgga aaccttgccg ttgttaccag
     9001 ccctggagca gttccgtaat gcccgcacgc actttgcttt tgtggtggat gagtttggct
     9061 cggtggaagg gattgtgaca ttaagtgacg tcactgaaac cattgccggt aacttaccga
     9121 acgaagtgga agagatcgac gcccgccatg atattcagaa gaatgcggac ggttcctgga
     9181 cggcgaatgg tcatatgccg ctggaggatc tggtgcaata tgtgccgctg ccgctggatg
     9241 aaaaacgtga atatcacacc attgccgggc tgttgatgga gtatttgcag cgtattccaa
     9301 aacctggcga agaagttcag gtgggggatt atttgcttaa aacgttgcag gtggaaagcc
     9361 atcgcgtgca gaaggtgcag attataccgc tgcgtaagga tggagagatg gagtacgagg
     9421 tgtgatggta tcggccattt tacccaatgc tgcgtaacgc cttataactt cagcaatgaa
     9481 gcgttgtctg tggtaaacgg acatcacagc gggggtgtgg gtccggctgt aaatcgccga
     9541 atcgtttcct gtaggctggg gcagcccaca tggatgtggg ctgagggcgc gttttacagg
     9601 gatgttacct cgcgcccgta cccgatagcc gaaaggaata agatgaggga accgcgccag
     9661 cggcgattta ccgccgggag cccgggttgc cagggtggtg gcgattgagc caccctggca
     9721 cgttcaccgg cttgagcgtt ccatagtagc gaggaacatc aagtgaacgg aacagccttc
     9781 gatcttacat attcagaata taatagtgcc gccgcgaacg cctgatgcga cgctttacgc
     9841 gtcttatcag gcctacagga caggtcacct ggtaacctac atcttctcca gcaacttctt
     9901 cacatctttg ccattgcggg aatctttatt ccgctcggcc cagtcattca ggcgacgttt
     9961 cgcttcgtcc tgtagatgtt tgcgcagtaa ctgatccact tgcaggctgt aattgagttg
    10021 ctgccagttg ccataaaccc gcagcggtac tggcgtttct ttgaggaaat caatcagttt
    10081 gctttccccg ttccagccac ccacgacccg aatatcaaac tgggtgtcgc aggtttgatc
    10141 tgccagattc aacatgcctt cccctgtcag cgccagcaca ggcgattgac cttgcatgtc
    10201 gtttaacgtc acgacgccat ccttcaacgt caaatcggtg gtaaagcggt caagccgcgt
    10261 cacgttatcg aagttttcag cggccttcac atcaccgcca ttacgctcta ccgcttgctg
    10321 aatcatctgc tggaagttca tcccttccat acgtgtgtcg gtcatctcaa catgcgcctg
    10381 tccttgccag ttgtggcgga atgcgtcggc atctatgtca gcaccggaga aatcaccagc
    10441 cagtgacatt tttccggtca acgaaatcgg atagttaaac gccttcagaa tggtaccaat
    10501 ctcaacgttc tccagccgtg gctggaagtt tatccgcgga tttattgatg tcgcgtccag
    10561 cgtgcccggc agtgaaacct ttccaccgtt aagcttgccc tgcagttgag taatttccag
    10621 caaacccgac ttgttggtca tttgcgtggc aacatctgta aaattcattc cgcgccagcg
    10681 cacgttactg gcctgcaaca aaatatcagc tgtaaagcct tgcagtccct gataggccgg
    10741 ttcatcaata cgcgaagaaa tgaccgggcg cggcaacgtt gattggctct gcccctgctg
    10801 cgcggcaccg ttttcaccat tcgcggtttc attaagcggg atgaggttgt caagattcag
    10861 ttgcgggaat tgcagtctca gctgccattc cggtttctct gtcagcgtga cctgtgcttg
    10921 tccgctcagc gtactgtcat tggcggtcaa actaatttgg ttaaatgaaa ggcgtttatg
    10981 tgactcctgc cactgggact ggaaactccc ctgcccctga ataccttgtt ttggtaaatc
    11041 ggcaccctgc aactgccagt taatttgttc aatagccgct gttaaatcat gcggataatc
    11101 agaagcatct accgtaccgt taagggatat cgtgagatcg cgctgatcgc gattaacccg
    11161 cccggagaac tcaaatgagc cacgatgttg gggatcttgt tccatttgca ggcggatatt
    11221 gcggattgtc acctgctcgt catcttcatg ctggaacacc agaacgctgt ccgccacctt
    11281 aagactggat atatcaaacg accatccgcg atcgtctgac agatccggca aggtattgtc
    11341 gcgcggtgca accggagcgt cttcactgcg caccgcttcc gtctgcggcg tcagttggat
    11401 cactgcccct tttagcatca cctgcttaac gctcagttga tgactcagta gtggtaaaag
    11461 cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc tggcttgcgc cctgggcggt
    11521 gagagacatt cgcccggaga ggatactaag ctgcggccag acgtgccaac gcagtggccc
    11581 gtcgagctgc aattgataac cgctacgtgc agcaacttgc ttgaccatat agtcgcggaa
    11641 atcattcgga ttcaccagca acactaacgc agataacccg gccaccagca cgaccaagag
    11701 tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca atgggcctga attagtcttt
    11761 atcgattcgg ctggctaccg cgccctgctg gttgcgatat ttcgcatctt cacggcggtt
    11821 gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa ttaacatgcc
    11881 cggacgcagc gccagcggca gcttaccgga gttgtagaac tccagcacaa tgcaaccaga
    11941 ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc agccccagac gcgccaaaga
    12001 ggaacgcccg tccagccagc ccaccagatc ggctggcagc gtcaccgact ccagtgtcac
    12061 cgccagcgcc agctctcctg ggtgaagata gaacgcctcg ccctcgtcga gaacgatctc
    12121 atcgctcatc acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc tcagatcgat
    12181 aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg cccaggcgta catccaccgt
    12241 cgcgccgtta atacgctcca ctggcggacg tgggttgatc gacaaacggc cttcatcaag
    12301 ccaggcttca atatctcggt cacacagacg catggcattt tctcctttcg cacatcactc
    12361 ccttaacgcc aattacgtca agggcatacc aacttatcac tgaacggtac acaatttatc
    12421 aagcttattc aaagaactga cttattttcg ctttcaatat atcgatcgcg atgcggtttt
    12481 tcccgccgcg cggcacgata atgtccgcat attgtttaga aggctcaatg aattgcagga
    12541 acatcgggcg cacggttttt tgatattgcg ccatcacgga atccattgaa cgcccacgct
    12601 cgttaacgtc acgcttgatg cggcgcatca ggcagatatc cagcggggta tcaacgaaaa
    12661 tggagaagtt aagttcgtca cgcaaacgcg catccgtcag caacaaaatg ccttcgagaa
    12721 tgatgacctt cttcggctca accgtcaccg tttctttcat acgcgtatgt tcaacatagc
    12781 tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc ttgtaaatgc tcaagcaaca
    12841 ggctgtgatc catcgcgctg ggatggtcgt agttggtctt aacgcgttct tccatcgaca
    12901 gatggctttg atctttgtaa tagcagtctt cgggaattac gccgatgtgt tcatcaccga
    12961 cttgctcacg taattcacga taaagggtac tggcaataag actcttgccg gaagccgatg
    13021 cgccagcgat accgataatg acgcactgat gagactgatc agtcatatat ttagcgacct
    13081 gattaacctg gatgttagga agggggcgac gaagcgccaa acgcggcaat tatagggatt
    13141 tcatccgtct gataccagtc gaatagcgtt gccgcgcgct cagagttaat tgttgacaaa
    13201 gaattcccgg tggcaaatta cgttgatcag ttttatgtaa ggtaaaaaat gttatacgca
    13261 gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa ttcgcatata aaatgtaaaa
    13321 cttttgtact agcataaaca cagaaacgaa tactggcgac ctagccttgc ggataaagcg
    13381 gtaataagca aacaatcaca gcatgtatta attgccctgc cccacccgct gcttcacctg
    13441 gtcagtttag gtttagtctc gtttatcttt acccttgtct cgcttgagct ttcgcagttt
    13501 ggcacccaac tcgccccact gtggttccca acgtccatca tgatggtggc gttttatcgc
    13561 catgccgggc gcatgtggcc gggaattgcg ctgagctgtt cgctgggaaa tatcgccgca
    13621 tccatcctgc ttttttccac cagctcgctg aacatgacct ggacgaccat caatattgtt
    13681 gaagccgtgg tcggggcagt gctgctacgt aaattgctgc cgtggtataa ccccttgcaa
    13741 aatctggctg actggctgcg tctggcattc ggcagcgcca ttgttccgcc tctgttgggg
//
LOCUS       DAJVPP010000101        13565 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVPP010000101 DAJVPP010000000
VERSION     DAJVPP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.101"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..3417
                     /gene="nifJ"
                     /locus_tag="OQM34_003176"
     CDS             <1..3417
                     /gene="nifJ"
                     /locus_tag="OQM34_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1453518.1"
                     /translation="DAWAGNGLKNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSF
                     TSSQGLLLMIPTLYKLAGELTPFVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLC
                     AANVQEAQDFALISHIATLKSRVPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEI
                     DAHRARALNPEHPVIRGTSANPDTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQY
                     QPFEYYGHPQAERVIILMGSAIGTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQA
                     LPGSVRSVAVLDRTKEPGAQAEPLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKE
                     FGPDCVLAVFAELNAAKPKARFTVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSD
                     GSVSATKNNIKIIGNSTPWYAQGYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADF
                     VGCHQLQFIDKYQMAERLKPSGIFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVIN
                     AAKIARECGLAARINTVMQMAFFHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNW
                     QALALARESVEEVPLQPVNPHSANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALP
                     PDGTWPMGTTRWEKRNIAEEIPIWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAP
                     ASLHSLDVKSRDMRGQKYVLQVAPEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEH
                     VEEEKINYDFFLNLPEIDRSKLERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQL
                     YGDRMLIANATGCSSIYGGNLPSTPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQ
                     HRVRVLRLLDQFADKIPAELLTALKSDATPEVRREQVAALRQQLNDVAEAHELLRDAD
                     ALVEKSIWLIGGDGWAYDIGFGGLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLG
                     AVTKFGEHGKRKARKDLGVSMMMYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLII
                     AYSPCEEHGYDLALSHDQMRQLTATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEE
                     TLLHEQRFRRLNSQQPEVAEQLWKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            3784..4913
                     /gene="ompN"
                     /locus_tag="OQM34_003177"
                     /pseudo
     CDS             3784..4913
                     /gene="ompN"
                     /locus_tag="OQM34_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5054..5488
                     /gene="uspF"
                     /locus_tag="OQM34_003178"
     CDS             5054..5488
                     /gene="uspF"
                     /locus_tag="OQM34_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1453519.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            5664..6599
                     /gene="ttcA"
                     /locus_tag="OQM34_003179"
     CDS             5664..6599
                     /gene="ttcA"
                     /locus_tag="OQM34_003179"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1453520.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(6728..8095)
                     /gene="dbpA"
                     /locus_tag="OQM34_003180"
     CDS             complement(6728..8095)
                     /gene="dbpA"
                     /locus_tag="OQM34_003180"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1453521.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8573..9556)
                     /gene="zntB"
                     /locus_tag="OQM34_003181"
     CDS             complement(8573..9556)
                     /gene="zntB"
                     /locus_tag="OQM34_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1453522.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            9811..11043
                     /gene="dgcM"
                     /locus_tag="OQM34_003182"
                     /pseudo
     CDS             9811..11043
                     /gene="dgcM"
                     /locus_tag="OQM34_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11064..11627)
                     /gene="smrA"
                     /locus_tag="OQM34_003183"
     CDS             complement(11064..11627)
                     /gene="smrA"
                     /locus_tag="OQM34_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS1453523.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(11881..13335)
                     /locus_tag="OQM34_003184"
     CDS             complement(11881..13335)
                     /locus_tag="OQM34_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCS1453524.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(13332..>13565)
                     /locus_tag="OQM34_003185"
     CDS             complement(13332..>13565)
                     /locus_tag="OQM34_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1453525.1"
                     /translation="KAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKI
                     VQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3628 a         3401 c         3217 g         3319 t
ORIGIN      
        1 gatgcctggg ccggaaacgg cttgaagaac gtttggggag acacaccacg cgtggttgaa
       61 atgcagtcgg aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac gggtgccctt
      121 tcaacatcgt ttacgtcatc gcagggtttg ctgctgatga tcccgacgct gtacaaactg
      181 gcaggcgaac tgacgccatt tgtcctgcat gtagcggcac gtaccgttgc cacacatgca
      241 ctttctattt ttggcgatca ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg
      301 ttgtgtgcgg ctaacgtcca ggaagcgcaa gactttgctc tcatttcgca catcgcaacg
      361 ctgaagagcc gcgtgccatt tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc
      421 aataaaattg tcccgctggc cgatgacact attcttgatc tcatgccgca ggccgaaatt
      481 gatgctcatc gcgcccgggc gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc
      541 aatcctgaca cttatttcca gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat
      601 gaccatgttg aacaggcgat gaatgatttc gctgccgcga caggtcgtca gtatcagccg
      661 tttgagtatt acgggcatcc gcaagcggaa cgggtgatta tcctgatggg ctctgccatt
      721 ggcacctgtg aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg
      781 aaagttcgtc tgtaccgtcc cttctccgcc aaacacttgc tgcaagctct gccgggatcc
      841 gtacgcagcg tggcggtact ggacagaacc aaagaacccg gtgcccaggc agaaccgctc
      901 tatctggatg tgatgaccgc actggcagaa gcctttaata atggtgagcg cgaaaccctg
      961 ccccgtgtca ttggtgggcg ctatggtctt tcatccaaag aatttggccc ggactgtgta
     1021 ctggcggtat ttgccgagct caacgcggct aaacctaaag cacgctttac ggttggtatt
     1081 tacgatgatg tgaccaatct gtcactgccg ttgccggaaa acactctgcc aaactcggcg
     1141 aaacttgaag ccttgtttta tggccttggt agtgatggca gcgtttccgc aaccaaaaac
     1201 aatatcaaga ttatcggtaa ttccacgccg tggtacgcac agggctattt tgtttacgac
     1261 tccaaaaaag cgggcggcct gacggtttct caccttcgcg tgagcgaaca gccgattcgt
     1321 tccgcttatc tcatttccca ggctgatttt gttggctgcc accagttgca gtttatcgat
     1381 aaatatcaga tggctgagcg tttaaaacct agcggcattt tcctgcttaa tacgccgtac
     1441 agcgcagatg aagtgtggtc gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa
     1501 aaagcgcgct tctatgtgat taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc
     1561 cgtattaata ccgtcatgca gatggctttt ttccatctga cgcaaattct gcctggcgat
     1621 agcgccctcg cagaattgca gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat
     1681 ctggtggagc gcaactggca ggctctggcg ctggcgcgtg aatccgtaga agaagttccg
     1741 ttgcaaccgg taaatccgca cagcgccaat cgaccgccag tggtttccga tgccgcccct
     1801 gatttcgtga aaaccgtaac cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt
     1861 tcggcgctgc cgccagacgg cacctggccg atgggcacca ctcgctggga aaaacgcaat
     1921 atcgccgaag agatccccat ctggaaagag gaactctgta cccaatgtaa ccactgcgtc
     1981 gccgcttgcc cacactcagc tattcgcgca aaagtggtac cgcctgaagc gatggaaaac
     2041 gcccctgcca gcctgcattc gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat
     2101 gtcttgcagg tggcaccgga agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg
     2161 gcgaaagacc gtcagaatcc agagattaaa gccatcaata tgatgtctcg ccaggaacat
     2221 gtcgaagaag agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc
     2281 aaactggaac gtattgatat tcgtacatcg cagctgatta caccgctgtt tgaatattca
     2341 ggtgcttgcc ccggttgtgg cgagacgccg tatattaaat tgctgactca gctctatggt
     2401 gaccggatgt tgatcgctaa cgccaccggc tgttcttcaa tttatggcgg taacctgccc
     2461 tctacaccgt ataccaccga tgccaacggt cgtgggccgg catgggcgaa ctctctattt
     2521 gaagataatg ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc
     2581 gtgctgcgtc tgctggatca atttgccgat aaaatcccgg cggaattact gacggcgttg
     2641 aaatcagacg ctacgccaga ggttcgtcga gaacaggtcg cagctttacg ccagcaactc
     2701 aacgatgttg ccgaagcaca tgaactgctg cgtgacgcag atgcactggt ggaaaaatca
     2761 atctggctga ttggtggcga cggctgggct tacgatatcg gctttggcgg tctggatcat
     2821 gtattgagtt tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg ctattccaac
     2881 accggtggtc aggcgtcgaa agcgacaccg ctgggtgcag taactaagtt cggcgagcac
     2941 ggcaaacgta aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg tcatgtttat
     3001 gtggcgcaga tttctctcgg cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg
     3061 gaagcgtatc cgggaccatc gctgatcatt gcttatagcc cgtgtgaaga gcatggttac
     3121 gatctggctc tcagccacga ccagatgcgc caactcactg ctaccggctt ctggccgcta
     3181 tatcgctttg atccgcgtcg tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc
     3241 ccgccgtcag aagcactgga agaaacgtta cttcacgagc agcgtttccg tcgtctgaat
     3301 tcgcagcagc cagaagtggc agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa
     3361 cgctatgact tcctagcaca aatggccgga aaagcggaaa aaagcaacac cgattaaatg
     3421 ctctggataa ggattatcca attctaaaaa aaaagcccgg acgactgttc gggcttgtct
     3481 ttttatatgt tggaaaatca gtggcaatgc aatggcccaa cagaaaataa aatctgtgac
     3541 aataaaggca tataacccgc gcagaataac gtataggaaa ttaaaagtat aatttttatt
     3601 ttgtatgtat tatcttcgat acattcacca gacttattat catttcagag gaattatttg
     3661 attaaggttt tacttaaggc gtaacaaatg atatttatcg gctaactgaa cttctccttt
     3721 aggatgtttt cactccccga aatggggata aaaggcaaat aaaataacta aaggatttat
     3781 tcaatgaaaa gaaaagtact ggcactttta attcctgccc tgctcgccgc aggtgctgca
     3841 catgcagccg aagtttataa taaagacggc aacaaattag atctgtatgg caaagttgat
     3901 ggcctgcatt atttttctga taattcagcg aaagatggcg accagagcta tgcgcgtctg
     3961 ggttttaaag gcgaaaccca aattaacgat caacttactg gctacggtca atgggaatac
     4021 aatattcagg caaacaacac tgaatcttca aaaaaccagt catggacccg tctggcattt
     4081 gccggactga aatttgcaga ttacggttct ttcgattacg gacgtaatta tggcgtaatg
     4141 tacgacatcg aaggctggac cgatatgctg cctgaatttg gcggtgactc ttataccaat
     4201 gcagacaact ttatgactgg tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc
     4261 ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc
     4321 agtaatggtc aggaaggcac caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc
     4381 tggggtcttt ccacaacata tgatttaggc atgggcttta gcgctggtgc ggcatacacc
     4441 tcttctgacc gcaccaatga ccaggttaac catactgcgg cgggtggtga taaagcagac
     4501 gcgtggactg ctgggctaaa atacgatgct aacaatattt acctagcaac catgtattca
     4561 gaaacgcgta atatgacccc gtttggcgac agcgattatg cagttgcgaa caaaacccag
     4621 aactttgaag ttactagtac cagtttgatt ttggtctgcg tccggcagtc tctttcctga
     4681 tgtctaaagg ccgtgacctt cacgctgctg gtggtgcaga caacccggca ggtgttgatg
     4741 ataaagatct ggttaaatac gccgatgttg gcgcgactta ctatttcaat aaaaacatgt
     4801 ccacctatgt tgactataaa atcaacctgt tggatgaaga tgacagtttc tacgctgcca
     4861 acggcatctc taccgatgat attgtcgctt taggtctggt ttatcagttc taaatcctcc
     4921 tgcccgctgt tatggcgggc tttttctgct tattcttcct cctttgatct aaattaaaaa
     4981 tgtgaactcc gtcattacac aaaaagtgtc atctggcgtt acactttatg cggatactaa
     5041 aacaggaggt tttatgaaca gaacgattct tgtccctatc gatatttcag attcagaatt
     5101 aactcaacgc gtgattagcc acgttgaggc agaggcaaag attgatgatg cagaggttca
     5161 tttcctgacg gtaatacctt cactgcccta ctatgcctct ctgggtttag cgtattccgc
     5221 agaattaccg gcaatggatg acctgaaagc ggaagccaaa tcgcaactgg aagagatcat
     5281 taaaaaattt aaactgccaa ccgacagagt gcatgtccat gttgaggaag gctcgcccaa
     5341 agaccgcatt ctggaattgg cgaagaagat ccccgctcat atgatcatca ttgcttccca
     5401 tcgaccggat atcaccactt atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc
     5461 agagtgctcc gtgctggttg tgcgctgaca ctaacgcccg cacattgctg cgggcttttt
     5521 gattcatttc gcaaatgtgc tgacattttc ccctctaatc cgtaccatac acgccacagt
     5581 ttttatatca gatttcttat gctgagcgtt ccggcatgac gcatactctt ctgatgccat
     5641 ataacgaatt gagtcgcttt taaatgtcgc aaaatcaaga aattagtaag aaagaacaat
     5701 acaacctgaa caaattacaa aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact
     5761 tcaatatgat tgaagaaggc gatcgcatca tggtttgcct ctccgggggt aaagacagct
     5821 ataccatgct ggagattctg cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg
     5881 tggctgttaa cctcgatcaa aagcaaccgg gcttcccgga acacgttctg cccgagtatc
     5941 ttgaaaagct gggcgttgag tacaagattg ttgaagagaa cacttacggt atcgtgaaag
     6001 agaagattcc agagggcaaa accacttgct cactgtgttc tcggctacgt cgcggtatcc
     6061 tttatcgcac cgcaacggaa ctggggacga cgaagatcgc gttgggtcac catcgtgacg
     6121 atatcctgca aacgttgttc ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc
     6181 cgaaactgat gagcgatgat ggcaaacata tcgttattcg tccactggcc tactgccgcg
     6241 agaaagatat tcagcgattt gccgatgcaa aagcgttccc gattattccg tgcaacctgt
     6301 gcggttcaca gcctaacctg caacgtcagg tgattgctga catgttgcgt gactgggata
     6361 aacgttatcc agggcgtatc gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc
     6421 atctgtgcga taccaacctg ttcgatttca aaggcattac ccacggttct gaagtggtta
     6481 acgggggtga tctggcgttt gatcgcgaag agatcccact acaaccggcg ggctggcagc
     6541 cagaagaaga tgaaaatcag ttggatgagt tacggctgaa tgtggttgaa gtgaaataac
     6601 caggatagcg cccgatgcgc aagcgtatcg ggctactctt atggaggccg gataagacgc
     6661 ggccagcgtc gcatccggca atcccgaata agatgtttac tcttgcaccc ggcaattcaa
     6721 catttcatta ttttaataac cgcacccggc acgtttttcc tttaatcttc ccgccctgta
     6781 actgtttcca tgctttatga gcaacagcct gacggaccgc gacatagaca tgcgccggat
     6841 gcacggcgat tttgccaata tctgcgccat caagcccgat atctcctgtc agtgcaccta
     6901 atacatcacc cgggcgcatt ttggcttttt tcccgccatc gatacacaac gttgccattt
     6961 ctgcttccag cgtcacaatg gaactattag ctggcggcgt ttgccagtta agttttatct
     7021 gcaacatgtc agaaatgata ttggcccgct gtgcttcttc cggagcacag aaactgatcg
     7081 ccagaccgct atttcctgca cgagctgtac gaccgatgcg atgtacatga acttcagggt
     7141 cccacgccag ctcaaagttc accaccagct caagcgattt aatatccaga ccacgcgcag
     7201 caacatcagt cgcaaccagt acacgggcgc taccgttagc aaaacgtacc agggtctgat
     7261 cgcgttgctc cagatcgccg tgtaacgaca atgcactttg ctctacttca ttcagcgcgt
     7321 cgcagacagc ctggcaatct tttttggtat tgcaaaacac cacacaagag gatggctgat
     7381 gcaagcttaa taaccgttgc aacagaggaa ttttgccttt actggatgtc tcataaaatt
     7441 gttgttcaat gggtggcaaa gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt
     7501 gcactcgtcc gctgattgcg gcgatggctt ccggccaggt tgccgaaaac agaagcgtct
     7561 gtcgagatgc aggcgcaaaa cggatgacat catcaatggc atcgctaaat cccatatcca
     7621 gcatgcggtc ggcctcatcc atcaccaaag tattcagcgc atccagtgat accgtgcctt
     7681 tttgcaagtg atccagcaaa cgacccggcg ttgccacgat aatatgcggc gcatgttgca
     7741 gcgaatcacg ctgcataccg aacggttgac caccgcacaa cgtcaaaatt ttggtatttg
     7801 gcagaaaacg cgccagccga cgcaattcac ctgccacctg atccgccagt tcacgcgtag
     7861 gacacagcac taaagcctgg gtttgaaata gcgacgcatc aatttgctgt aacaagccga
     7921 ggccaaaagc cgccgttttg ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg
     7981 caaggatcgc cggaagcgcg gcggcctgca ccggcgtcat ggttaaataa cccaactcat
     8041 taaggttcgt gagttgggcg ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct
     8101 attctcgtgg tcatcgacgc aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg
     8161 atgcgacaat ttaatcggtt cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg
     8221 gatcggatcg ggcactggaa caggatcgcc aggaatcggt tcagggacag gaatttgcaa
     8281 ataaataagt gtcgtcatat ttccctccgg tcattgggtg gactcttaaa gggtagacgc
     8341 tgataaataa caggcaaaaa aaagccgact catcaaagtc agcgtcgtac gaatcaattg
     8401 tgctatgcag taattcaaaa aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg
     8461 acgttgcatt cacctgcaag ggagatattg ccctgaatgg gtagagagtt tattgacttc
     8521 gctcaaactt tgcggcgttt ttgtatacag acagccggaa aaattgcttt tgttacaacc
     8581 atttactacg gtgcaaccat aaagcaacac caccaataag aacaactaac agaatacaaa
     8641 aaattgaaaa tccgaattgc cacccgccgc cagggatccc accaaggttg acgccaaata
     8701 acccggtcag aaaggtactg ggtaaaaaga ccattgccat caacgacatt gtataggtac
     8761 gacgagctaa attttcctgc atcacctgag cgatttcatc cgccatcacg ccagtccgtg
     8821 ctatacaggc gtcgatttcg tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc
     8881 gacggcgttg gtcatcgctc atccacggca aacgttcact ggcaagacga gcataaacat
     8941 cacgttgcgg tgccatatag cgacgcatca caattaattg tttgcgcagc agagccagga
     9001 atccacgcgg tggaatttgc tgatcaagga gattatcttc aaggtcgata attttatcgt
     9061 gcagctgctc gataaattca ctggaatgat cggtcaacgc atcgcacaca tccaccagcc
     9121 atcccccgca atcggtcggc cccgtgccct cttccagatc gctcaccaca tcgtccagcg
     9181 ccagcacttt gcgttgtcgg gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg
     9241 cgaccagttg atcggggcgt tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg
     9301 tgccttcacc gaaacggctg actcggggcc gtgtgctctc gcccgccagc gcatcacgta
     9361 cgttattggg aagcagcggt gttgtcgcca gccattgggc gctatcatgg tgtacataat
     9421 taaggtggag ccagcaggga tgcgcttcat caatcacatc tgtattttcc agcggtttaa
     9481 cgccgcctct accatccagc atccaggcaa atactgcatc cgggacatta acgtccgatc
     9541 ccttaatcgc ttccacagtg cctccatcat caacgcatta ttttgtagtc tagccttctg
     9601 gccctgttac gcaacatctc atcaccccat taccctgaaa tgattaataa aattctgtct
     9661 aaattgaata caaaaagcaa aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag
     9721 cgtctatcat tatcagaatt atctgatcat atgacgtggc ttttttgcga tcggatagca
     9781 acaaaaattg ataaaaataa cgggatctca atgattacgc acaacttcaa taccctggac
     9841 ttactcacca gtcctgtctg gattgtttcg ccctttgagg aacagttaat ttatgccaat
     9901 agcgcggcgc gactgttgat gcaagacctc acgtttagtc agctacgaac cggaccctat
     9961 tccgtctcct cacaaaaaga actgccgaaa tacctctccg atctgcaaaa ccaacacgat
    10021 attatcgaaa tcctcactgt tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt
    10081 gttttgcgag agctgacaga aacagaaccg gtgattattt tcgaaggtat cgaagcgccg
    10141 gcaacgctgg gtttaaaagc cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt
    10201 tatgcgcgct tttttctgac taactctgca ccaatgttgt tgattgaccc gtcacgagat
    10261 ggacaaatcg tcgatgctaa cctcgccgcg ctcaatttct atggttataa ccatgaaacg
    10321 atgtgccaga aacataccta ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg
    10381 catgaaattt cgcatttacc cggcggtcat aaccctttga attttgttca taaactggcg
    10441 gatggatcta ctcgtcatgt gcagacctat gccggaccga ttgaaattta tggcgacaag
    10501 ctcatgttat gtattgtgca tgatattact gagcaaaaac ggctggagga gcagctggaa
    10561 catgctgctc accatgacgc gatgaccgga ttactgaatc ggcgacagtt ttatcacatt
    10621 acggaaccag gccaaatgca gcatctcgcc atcgctcagg attacagctt gttgctcatc
    10681 gacaccgatc gttttaaaca cattaacgat ctctatgggc attctaaagg tgatgaggtg
    10741 ttatgcgccc tcgcccgccc cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt
    10801 tggggaggcg aagagtttgt cttattgcta ccaagaaccc cactggatac cgcgctttcg
    10861 ctggctgaaa ctatccgcgt aagcgtggca aaagtgagta tttcgggctt accacgcttt
    10921 accgtcagca ttggtgtggc gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa
    10981 cgcgttgatg atgctttgta tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca
    11041 taagccgcgg atgcgtctcg agatcaacga ctgcgcttag cgtggcgctc ccagttttct
    11101 tgcttcgcct gcgccgtttt acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc
    11161 ggtagcgcgg tgcaatatgc ctgaacatca tcaaattcgg tcagccagcg cgccacatag
    11221 ctgcggacaa tattggcatg tgatttatct tcccgccctt taccatgaat aatcagcaca
    11281 ttacgcaaac catccgccag ggcttgttga atgaaactga acaccatttt gcggcactct
    11341 tccaccggct ggcgcaaaag attcaggctc gcctgttgcg gatatttacc actgcgcagc
    11401 ttatccagca ccccatgctg caacccttcc tgccgaaact ccagcggctg acttagcggg
    11461 atgatgtcga gaaatccggt agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc
    11521 ggcgcacgtt ggttacgcgt tggatgccag tggacatctg tagcacgttt cagcggctgg
    11581 acatcttcca tggcgtcaag aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg
    11641 caccttcaca cgtcgtgatt cagacgggtt aattgttcat acttactgca tcgttccctt
    11701 cacagattga aacaatactc tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt
    11761 atactagcct gttagcagta tcgaacagtt tagcccgagt ttaattcagc acaggctccg
    11821 atcaggatga tttattcata gcaggaacca acaaaaatgc gataaataac agaccactga
    11881 ttagcagaaa ggtaccgaaa atgccatagt ttgcgcctcc ctcagcatca attgactcaa
    11941 taatttggtt gaagccagta tttccgatgt ctggtcactt ctactgcgat taaaaaataa
    12001 agcacttctg gaaatgaagt cattcaccgt atggggaaca gaaaccattt tttcacccat
    12061 tctcgaaaaa tgaaaattag ttcgattatt gagaatagag ccacacaggg caataccaat
    12121 cgcgccccca agattacgtg ttaaattaaa tacaccagag gctaacttca gccgttcttt
    12181 tggaattccc cccagcgtta atgttacgat gggggccatc gcaaactgct gcgaaatccc
    12241 tctaattgcc tgtggaaaca gtaattcctg ccatccccat tcgtgggtta taggggtaaa
    12301 aagatacatt gaaaacacaa atccgcccag ccccgccatc aacagccatc gcagatttat
    12361 ttttttactt aaccagaagt aaaagggaac agaaaataac tggaaaatac ctgttgtgca
    12421 aacggcaaaa cctatctctt cggcatttaa tccgcgaatc tgccccaaaa acacaggtat
    12481 taaatataca gtagagaata tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt
    12541 gaaattttta tccttaaaag catgaagatc cattatcgga ttggatattg taagtgtacg
    12601 cgcagcaaat aaaataaaac tcaccaatgc cagaactgat gtcagcaaaa tagtattatc
    12661 atccagccat ccccagcgcg ctccttcttc aagggtatat tccagacatc ccagagtcat
    12721 ggccaacagg ataatactcg gataatcagc aactttcagt aaagagagat caggtttatc
    12781 aaaattaacc agaaaaggaa tactcaaaac taaatatatg ccaggtaata tattgatata
    12841 aaatagccat cgccagtcga ggttatcagt gagccagccc cccagagttg gccctagcgt
    12901 cggtgacagt gaggccagcg cactgactac cgcagcagcc aaccccaact ctttaccctg
    12961 gtaataaata aacgccatcg tgaaaactaa agggatcatg gatgcgccag cagctccctg
    13021 taaagcacga aacaaaatca tgatctgaat attccatgca agtccacacg cgatactcat
    13081 taaagtaaat attcccgcgg atagagtaaa aagccaacga gtggaaaata cccgtgacag
    13141 ccacccagag agcggaataa caataatctc agcaattaaa tagctggtct gaagccaggc
    13201 catttcgtct ttacctgcgg acagaccacc acctatttca tttagggatg acgatacaat
    13261 ttgaatatca agctgagcaa taaataatcc catacacata ctggcgaaag caatatgttt
    13321 acgcatagac atcatctctc atcaacactg acggtgacgg acagtcccgg gcgaagaagt
    13381 tgctttaatt cctttgcgtc gtcaaatgca atccgtaccg ggacacgctg aacaattttg
    13441 gtgaaattgc cagtggcatt ttctatcgga atcaagctga aactggctcc tgtcgctggg
    13501 gaaaggcttt caatgtgacc gtgaaacact tcccctttga gaatatcagc acggatctca
    13561 gcctt
//
LOCUS       DAJVPP010000102        13268 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVPP010000102 DAJVPP010000000
VERSION     DAJVPP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.102"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..2768
                     /locus_tag="OQM34_003186"
                     /pseudo
     CDS             1..2768
                     /locus_tag="OQM34_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2778..6424
                     /locus_tag="OQM34_003187"
                     /pseudo
     CDS             2778..6424
                     /locus_tag="OQM34_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6485..6892
                     /locus_tag="OQM34_003188"
     CDS             6485..6892
                     /locus_tag="OQM34_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453526.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7434..8522
                     /locus_tag="OQM34_003189"
     CDS             7434..8522
                     /locus_tag="OQM34_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCS1453527.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVITVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8533..10784
                     /locus_tag="OQM34_003190"
                     /pseudo
     CDS             8533..10784
                     /locus_tag="OQM34_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10777..11913
                     /locus_tag="OQM34_003191"
     CDS             10777..11913
                     /locus_tag="OQM34_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1453528.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            11910..>13268
                     /locus_tag="OQM34_003192"
     CDS             11910..>13268
                     /locus_tag="OQM34_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453529.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYL"
BASE COUNT         3289 a         3490 c         3400 g         3089 t
ORIGIN      
        1 aatagtggcg actgatgagc acattcttgc ctcattagaa aaatatcacg agccatatgc
       61 catttttgat gattactatt gtggtgcgat atggtcagcc accgtattac aggagcaagg
      121 tgttgcagcc ctgccccgat ttgctcccta tgccgcaagt gactactgcg ccgatgtgtt
      181 gcgtcatatc aatcatccgt tcgcattaac actgcttata cgtgtagccg ggcatactaa
      241 acgctgtcac gatcgaatga cgaaagcctg cgctgcgttc ccacacgcag cactggcagc
      301 actggcggaa cttctagtgc aaaaagaaga gaatagttgg cgcattatgc taatgacgat
      361 gtttatctcg caaccagcac tggcagaaca ggtcattccc tggctttcga cacccgcagt
      421 tgcagtgctg aaatcatgcc agcaccaact gacacagccc tcaaaccatg ccagcgccga
      481 tctactgcca gccatagtag tctccccacc atggatttcg aaaaagaaaa aatcgccgat
      541 tccggtgctg gatttagcgc cattaggcat cgagccaatc tgttatctga cagaagaaat
      601 cagtaatcaa cttttggcga aatatatctg gtattcaaaa cacatcacgg ttagccatga
      661 agaaagtact accaacctgt tggcaaggat gggttttcaa cgacggatcg ctggtacata
      721 tattaaagct cccgaagcgg tagttgaggc atggctaaat gaagattatt caaccttact
      781 aagtgaattt aaggtgtttc attcacctac cgggcattat tggcagttgg ggattttgac
      841 aacattgccg ctggaggctg gagaaagcag taaaagcatg gaatgccctt actctatctc
      901 cacataccga taccgaatac gccatgttac attttggact caaaggttta cttggtttag
      961 ttaactcaat tacacgctat ccacaggaag ccttgcccat cacgaattac ttcgcagcga
     1021 gtgagctggc tcctgccgtc gcccgtgcct tcaacaaact caaaacgcta cgcgaaaacg
     1081 cccgcacctg gctgttgaaa tacccggaac atgcccttac cggcctgctg cctgcggcgc
     1141 tcggcaaagc cggtgaagca caggataacg cccgcgctgc cttgcgtatg cttaccgaaa
     1201 acggtcatca gccattactg caagaaatcg cccggcgtta taaccagtcg gaagtaacag
     1261 acgcggtgaa cgctctgctt gcgctcgatc ccttagataa tcacccgaca aaaatcccca
     1321 ctcttccgac cttttatcag ccatcgatct ggacgcgccc ggtattaaaa gcaaacgccc
     1381 aatcactgcc agataacgcc ctcctccacc tcggcgaaat gctccgcttc cctcaggaag
     1441 aggctctgta tccgggatta ttgcaggtga aagacgcctg taccgctgac tctctggctg
     1501 aatttgcctg ggatctgttt accgcctggc agaccgctgg cgcgccgtcg aaagagagtt
     1561 gggcgttcac tgcgttaggc gttctcggta acgatgacac cgcccgcaaa ctgacgccat
     1621 taatacgcgc ctggcctggt gaatcccagc ataaacgcgc caccgttggg ttggatattc
     1681 tcgccgctat cggtagtgat atcgccctga tgcagcttaa cggcattgct cagaaactga
     1741 aattcaaagc attacaggag cgggcaaaag aaaagattgc cgacattgcc gagagccgcg
     1801 aactcacggt agcggagctt gaagatcggt tagcaccgga tctcggtctg gatgataacg
     1861 gttcgttgct gctggatttt ggcccacgcc agttcaccgt cagctttgat gaaaccttaa
     1921 aaccgtttgt gcgtgatgtt tccggcagcc gcctgaaaga cctgccaaaa ccaaacaaaa
     1981 gcgatgatga gtcacaggcg aacgatgctg ttaaccgcta caaattgctg aaaaaagatg
     2041 cgcgtaccgt cgccgcccag caggtagcaa ggcttgaatc cgccatgtgc ctgcgtcgcc
     2101 gctggtcgcc ggaaaacttc caactcttcc tggttgtgca tccgctggtt cgccacttaa
     2161 cccgccgtct gatttggggc gtttatagcg ccgacaacca gctactggcc tgtttccggg
     2221 tagcggaaga taacagctac agcaccgctg acgatgatct tttcaccctg ccggaaggcg
     2281 atatctctat cggcattcct cacgttctgg aaatatcacc gacggatgct gtcgcctttg
     2341 gtcagctttt tgccgactac gaactgctac caccgttccg tcagcttgac cgtaacagtt
     2401 acgccctgac agaagccgag cgcaacgcca gcgaactgac ccgctgggca ggcagaaaat
     2461 gcccgagcgg ccgggtcatg gggctggcaa ataaaggctg gataaagggc gaaccacagg
     2521 atggaggctg gatgatcaaa cctctcggtc gctggtcgtt aatcatggaa atcgacgaag
     2581 gttttgcggt gggtatatca ccagccgaac ttagcgctga gcagctctta agcaagctgt
     2641 ggctatggga aggcaaagcg gaaagctatg gctgggggag taattcaaca caggaagcgc
     2701 agttctccgt actcgatgcc atcaccgcca gcgagctaat taacgatatt gaagccctgt
     2761 ttgaataagg aaagagcatg gacaaggaat taccgtggct ggcggataac gcccaactgg
     2821 aactgaaata taaaaaaggc aaaacaccac tcagtcatcg caattggccg ggcgaaccag
     2881 tgcctgttat cactgaaagc atcattcaga cactgggtga tgaattgcta caaaaagctg
     2941 agaagaaaaa aaacattgtc tggcgttatg aaaatttttc accggagtgg cagtccgcca
     3001 ttacgcaggc catcaacttg atcggcgaac acaaaccctc agtcccggcc cgcacaatgg
     3061 cggcactagc ctttatcgcg caaaatgaca accaacagtt gctcgacgaa atcgtccaac
     3121 aagaggggct ggaatatgcg actgaggtgg tgattgcacg ccagtttatt gcacggtgtt
     3181 atgagagtga tcctctggta gtcacattgc agtatcagaa cgaggactat ggctatggtt
     3241 atcgctcaga aacctataac gaattcgatc tccgactgcg taaacacctc tctctggcag
     3301 aggaaagctg ctggcagcgt tgcgccgaca aactcattgc cgcactgcca ggaataacca
     3361 aagttcgccg cccttttatt gcgctgatcc tccccgaaaa accagaaatc gccaatgagt
     3421 tggtaagcct tgaatgcccg cggactcatt ttcattctaa ggagtggtta aaagttgttg
     3481 ctactgaccc caaagcggtg agaaaactcg aacgctactg gagccaggat atatttagcg
     3541 atcgagaagc cagctacatg tcgcatgaaa accacttcgg ctacgcggcc tgcgctgccc
     3601 ttttgcgcga acaaggactg gcagtcgttc cgcgcctcgc gatatatgcc cataaagaag
     3661 attgcggcag tctgctggta caaattaacc atccgcaagt catccgcacc ttgctgttag
     3721 ttgctgataa aaacaaaccc agcctgcaac gtgtggctaa atacagtaaa aacttccccc
     3781 atgcgacact cgccgcactg gcagaactgc tggcgttaaa agaaccacca gcccgcccag
     3841 gttatccaat catcgaagac aaaaagctgc ctgcacagca aaaagctcga gatgaatact
     3901 ggcgtacgct gttacaaacg ctgatggcat cgcagccaca actggcagca gaagtgatgc
     3961 cgtggttaag tactcaagcc cgggcagtgc tgaatagtta tttatcggca ccgcccaaac
     4021 cagttattga tagcaccgat aacagccaaa tgcctgaaat cctcgtttca ccaccatggc
     4081 gtgggagaaa gaaaaagact tccatacgtc acttttcgct gcctgatctt acgcttgcac
     4141 cgcaaacgta caggtcgccg aataaacact ggacgccgga acagcaaact gcaattgacc
     4201 attttagcgc catgtctttt gacgaacgtc ttgctacgag aggagcggat atcttcttgc
     4261 gggaattagg ttttgacgtt catagcctgg aatttaataa gcgtcttctt aatggacagt
     4321 tggatgcctt tgatgctcag caactgcaag aatacacatc aataggacta ttgcgtactt
     4381 atcacgcgca tcaggccaaa tctaaacagt accgggaaga cgccaccaat gcattactgg
     4441 cacaagatag cattgcgttg atgggagggt ggcgaacttt tcagcacgga ttttactatg
     4501 gtaatgaaca gcgttggggg atttggaatc tgtaccttat cgcgcagatg ccgcgcgaaa
     4561 tggcggtatc gtgctggcaa caaattgtcg aggctgattt tcattacacc ggggtggaat
     4621 atttgttaag tgtgctgggt accgatgctt tgcctggatt aaacaccgcc tttgcccgac
     4681 atccaaaaga gatatttccg ctgctgattc atttcggcgc aacagaactg gccctgccca
     4741 ttgcccgcgt ctggcacctt tttgcgggcc agcgtaatct ggctcgccag tggattttac
     4801 aatggccgga acatacggct actgcactta tccctcttgt cttcaccaaa tctagcgata
     4861 aaagcgaagc tgcattactt gccctgcgtt tactttacga acatgggcat ggcgaatggc
     4921 tacaaactgt agccaatcgt tggcagcgta cagatctatg gcctgccctg gaacaattac
     4981 ttaaacagag tccaatagag atttacccgg cacgcattcc aaaagcccct gatttctggc
     5041 atccgcaaat gtggtctagg ccgcgcctta tcactaataa tcaacctgtt accgatgacg
     5101 ctctggaaat tatcggcgaa atgctgcgct ttacccaggg gggacgtttt tatagcgggc
     5161 tggaacaact gaaaacgttc tgccagccac aaacgctggc agcttttgcc tgggatctct
     5221 tcactgcgtg gcaacaagct ggtgcccccg caaaagacaa ctgggcattt ctggcgttaa
     5281 gtctctttgg tgacgaaagc acggcacggg atctaacgac acatatcctc gcctggccac
     5341 aagaaggcaa atctgcccgt gcggtcagtg gcatgaacat ccttaccctg atgaataatg
     5401 atatggcgct gatgcagctg catcatatat cgcaacgggc gaaatcccgc cccttacgtg
     5461 ataacgcggc ggaatttctt caggtagtcg cagaaaatcg cgggctaagc caggaagagt
     5521 tagcggacag attagtccca acactgggcc ttaatgatcc gcaggcgttg agttttgatt
     5581 ttggtccccg gcagtttacc gttcgcttcg atgaaaacct caacccggtt atctttgatc
     5641 agcaaaacgt tcgccagaaa agtgttcccc ggttgcgcgc cgatgacgat caactgaaag
     5701 cgcccgaggc actggcccga ctaaaagggt taaaaaaaga tgctactcag gtgagcaaaa
     5761 acctgctccc gcgtcttgaa gctgccctac gtaccacccg acgctggtcg ctggcagatt
     5821 ttcattctct gtttgttaat catcccttta cccgtctggt tacccagcga ttaatatggg
     5881 gggttatccg gcaaatgaac cgcgtcgttt actcaacgcc tttcgtgtgg ccgcagaggg
     5941 ggagttctgc aatgcgcaag atgagccaat tgacctgcct gcggacgctc tgattggcat
     6001 tgcccacccg ttagaaatga cagcagaaat gcgcagtgaa tttgcacagc tttttgccga
     6061 ttacgaaatt atgccgcctt ttcgccagtt gtcgcgccgc acggtgctgc tcacacctga
     6121 cgagtcaacc agtaacagcc tgactcgctg ggaaggtaaa tccgctaccg ttgggcaact
     6181 tatgggaatg cgatacaaag gctgggagtc aggttatgag gacgcatttg tctatgacct
     6241 gggtgagtac cggctggtcc ttaagttttc acccggtttt aaccactaca atgttgatag
     6301 caaagcgcta atgagcttcc gttctcttcg agtgtaccgt gacaataaat ccgtcacttt
     6361 tgccgaactt gatgtgtttg atttgagtga ggcgttaagc gcacctgacg tcattttcca
     6421 ttaaaacaaa gcgggcagtg atgcgactgc ccgttatcta cacgacttac cagcggggaa
     6481 agcgatgatc gtgcaaaaag agctggttgc tatttacgat tatgaggtcc ctgtacctga
     6541 agatccgttt tccttcagac ttgagatcca taaatgctct gaattattta caggttccgt
     6601 ctaccgactg gaacggttcc ggctacgtcc aacatttcat caacgtgatc gagaggatgt
     6661 tgacccgcta ataaatgatg cgttgattta tataagagat gagtttattg atgagcaaaa
     6721 attacgaggc gaatcacctg aacctgtaat agtaattttt aattgtaaac tacagaatat
     6781 attcaaccaa caaatagaat tatatactct agataattca agtcgcagca catgcaactt
     6841 gaagtatgac gagtatagct ttattaaagg ggggaacggt attccccctt agatgcgttt
     6901 caatttgccc gttacttcaa caaatcaatc aaccgtaaca gagccactaa tagctccagt
     6961 acagcaatga gcacggttaa cttttgtgtc agcgtgtcca tccgctctcc ttattaagga
     7021 gcacaacacc agcaccttac ccttacactg cctgtcgata ctggtttacg tttgttgagt
     7081 cgccatgttg cactccggat cagagcacta cctgcggcac cacccgtccg gtagccagga
     7141 cttgaaatag gttcgatccg gcgcaacttc ccaacagtca ggattataaa tctcattatc
     7201 ttaattgcaa cactgtccag aggtggttat aattcgttcg ttggaacacg atgttgcaca
     7261 ggcagtggtg ttaggcctga aaatagtaaa taaaagaacc tgcctcacca gcagtttttt
     7321 ttatttactg tgatctgctt tccagatatt tttcgctcaa acaactaatg cgccaaacat
     7381 ttattgcgcg taaaatatcg tttatttcat taatacattt cagggatgaa tatatgtcac
     7441 cgcagaataa tcatctgcag cgtccgcctg ctgctgtgtt atacgccgat gaactggcaa
     7501 aattaaaaca aaatgataac gcaccttgcc cgcccggttg gcagttaagt ttgcctgcgg
     7561 cccgtgcttt tatccttggc gatagcgcgc aaaatattag ccgtaaagtg gtgattagtc
     7621 cctccgctgt cgaacgtatg ttagtcactc ttgccaccgg acgcggtttg atgttggtgg
     7681 gggaacctgg caccgcaaaa tctctcctgt ctgaattact ggcaacctct attagcggcg
     7741 atgccgggtt aaccatccag ggcggcgcat ctactaccga agatcaaatc aagtacggct
     7801 ggaactacgc cctgcttatc aatcatggtc catcaacgga agcgctggtc cctgccccgc
     7861 tttaccaggg aatgcgcgac ggcaaaatcg tccgttttga agagataacg cgtacaccgc
     7921 tggaagtgca agactgtctg ttaggtatgc tttctgacag ggtgataacg gtgccggaac
     7981 tcactggtga agccagtcag ctctatgcgc gagaaggctt taatattatt gccactgcca
     8041 atacccgcga tcgcggcgtc aatgaaatga gtgctgcgct aaagcgtcgc ttcgattttg
     8101 aaactgtctt tccgattatg gatttcgccc aggagctgga gcttgtcgcc agtgcttcgg
     8161 cgcgtttgct ggcccatagc ggtattccgc ataaagtgcc tgacgcggta ctggagttac
     8221 tggtccgcac cttccgcgat ctgcgtgcca acggcgaaaa gaaaacgtca atggatacac
     8281 taacagcgat tatgtccacc gctgaagccg tgaacgtggc ccacgccgta ggcgtccgcg
     8341 cctggttttt agcgaatcgc gcaggtgagc cagccgacct ggtggactgc attgccggaa
     8401 ccatcgtcaa agataacgaa gaagatcgcg ctcgtctgcg ccgttacttc gaacagcgtg
     8461 tcgccacaca taaagaagct cactggcagg cttattatca agcccgccac cgcctgccgt
     8521 gaggaaagat gcatgagcga gccgttaatt gtcggcatcc ggcatcatag tccggcctgc
     8581 gcccggctgg tgaaatcgtt aatcgaaagc cagcggccac gatacgtgtt gattgaaggc
     8641 cctgctgatt ttaatgaccg ggtagacgaa ctgtttttag cccaccagct tccggtagct
     8701 atttacagtt attgccagta tcaggacggt gcagcccccg ggcgtggtgc ctggacgcca
     8761 tttgctgaat tttcgccgga gtggcaggcg ctacaagccg atcgtcgtat tcaggcacaa
     8821 acttacttca tcgatttgcc ttgctgggcg cagagtgaag aagaggacga ttcgcctgat
     8881 acgcaagagg aaagccagac cttgctgctg cgtgctggga tcacttgttc gaagatgaaa
     8941 gccagcaaac tgcattaccc tctgcactgg cgcactattt tgcccaactg cggggcgact
     9001 cccccggcga tgctctaaat cgtcagcgcg aagcctttat ggcccgctgg attggatggg
     9061 cgatgcagca aaataatggc gacgtgttag ttgtctgcgg tggctggcac gctccggcac
     9121 tggcaaaaat gtggcgcgaa tgtccgcagg taattaacac gccagaattg ccgtcgctgg
     9181 cagatgccgt tacaggttgt tatctcaccc cctacagtga aaagcgcctt gatgtgctgg
     9241 caggatacct ttcaggaatg cctgccccgg tctggcaaaa ctggtgctgg cagtggggct
     9301 tacagcaggc gggagaacaa ctgctaaaaa cggttctcac ccgtttgcgc cagcacaagt
     9361 tgcctgcttc gaccgcggat atggctgccg ctcatctgca tgcgatggca ctggcacagt
     9421 tgcgcggtca tacactaccg ttacgcactg actggctgga tgccatagca ggatcgctga
     9481 ttaaagaagc cctgaatgcg ccgttgccgt ggagctatcg cggcgttatt catcccgata
     9541 ccgatccgat tctgctaacg ttgatagaca cattagcggg tgacggattc ggtaaacttg
     9601 ccccttctac gccacaaccg cctctgccaa aagatgtcac ttgcgaactg gaacgtaccg
     9661 caatctctct tccggcggag cttaccttaa atcgctttaa ccccaatgga ctagcgcaaa
     9721 gtcaggtgtt acatcggctg gcaatactgg agatcccagg gattgtacgc cagcagggaa
     9781 gcacactgac acttgcaggc aacggtgaag aacgctggaa attaacccgc ccgcttagcc
     9841 agcatgcggc attgattgag gccgcctgct ttggtgccac actccaggaa gccgcacgcc
     9901 ataaattaga agccgatatg ctggacgcgg gtggaatcgg cagcattacc acatgtctta
     9961 gccaagcggc gtcagcgggt ctggcgtcct tcagtcaaca attactggag caactcacat
    10021 tattaatcgc ccaggaaaat caatttgccg aaatgggcca ggcgctggaa gtgctatatg
    10081 ccttatggcg gctggatgaa attagcggta tgcaaggcgc gcagatatta caaacgacgt
    10141 tatgcgcggc tatcgatcgc acgctgtggc tgtgtgaatc caacggcaga ccggatgaaa
    10201 aggagtttca cgctcacttg catagctggc aagcgttgtg tcatatcctg cgcgatctac
    10261 atagcggtgt gcaactcccc ggcgtttcgc tttctgcggc ggtagccttg ctggagcgac
    10321 acagtcaggc gattcatgtc ctggcactgg atcgcggcgc gactcttggt gcgctgatgc
    10381 gtctggaaca tcccaacgct agtgccgaag cggcgctgac gatgctggcg cagttatccc
    10441 cggcacaatc cggcgaggcg ctgcacggtt tgctggcatt agcccgtcat caactggcct
    10501 gtcagccggc atttatcgcc ggtttcagca gtcatttaaa tcaactaagt gatgccgatt
    10561 ttaccaatgc cctgcccgat ttacgcgcgg caatggcctg gctaccgcca cgagaacgcg
    10621 ggacgctggc gcatcaggtg cttgagcatt atcaactggc gcaacttccc gtttcggcac
    10681 tgcaaatgcc gttgcattgt ccaccgcaag ccattgcaca tcatcaacaa ctcgaacagc
    10741 aggcactggc atcgctgcaa cactggggag ttttccatgt ctgaactgaa cgatcttctg
    10801 accacccgtg agctacaacg ctggcgatta attcttggcg aagcggcaga aacgacgctt
    10861 tgtgggctgg atgacaacgc ccggcagata gatcacgcgc tggagtggct gtatgggcgc
    10921 gatcctgaac ggctccagcg tggtgaacgc tctagtggat taggtggctc aaatctcacc
    10981 actcctgagt ggatcaacag tattcacacg ctgtttccgc aacaggtgat tgagcggctg
    11041 gaaagcgatg ccgtactgcg ctacggcatt gaagatgtgg tgacaaatct cgacgtgctg
    11101 gaacgtatgc agccttccga aagcctgcta cgcgccgttt tgcacaccaa acatctgatg
    11161 aaccccgaag tactggctgc cgcccgcagg attgtgcgcc aggttgtgga agaaattatg
    11221 gcgcgactgg caaaggaagt tcgtcaggct ttttctggtg tccgcgatcg tcgtcgccgc
    11281 tcatttattc cactggcgcg aaactttgat ttcaaaagta ctctgcgcgc caacctgcaa
    11341 cactggcacc cgcaacacgg caagttgtat atcgaattcc cccgctttaa cagccgcatt
    11401 aaacgccaaa gcgagcaatg gcaactggtc ttactggttg atcaaagcgg atcgatggtc
    11461 gattcggtga tccactctgc ggtgatggcg gcctgtttat ggcagttacc cggcattcgt
    11521 acccatctgg tggcgtttga cacaagcgtc gttgatctca cggcagacgt tgccgatccg
    11581 gtagagttat taatgaaagt gcaattaggc ggcggaacca atatcgccag tgccatggag
    11641 tatggtcggc aacttattga acaacctgca aaaagcgtca ttatcctcgt gagcgatttt
    11701 tatgaagggg gttcatcatc attgctgacg caccaagtga aaaagtgtgt ccagagcggc
    11761 atcaaagtgc tgggactggc agcgctcgat agcacagcaa caccttgcta tgaccgcgat
    11821 acggcccagg cgctgattaa tgtcggcgca caaatagccg ccatgacacc gggcgagctg
    11881 gcatcatggc ttgcggagaa tcttcagtca tgaattcact acgtccggaa ttattagaac
    11941 tgacaccgca ggcactgacg gcgttaagca atgccggttt tgttaagcgc agccttaagg
    12001 aactggaaaa tggcaacgtc ccggaaatca gccatgagaa cagcgcttta atcgccacct
    12061 tcagtgacgg tgtccgtacc cagctggcga acggccaggc actgaaagag gctcagtgca
    12121 cctatggggc cagcggcatg tgccgtcatc gcgtgatgct ggtgttaagt tatcaacgac
    12181 tttgtgctac cgcccagcct acgggaaaag aagaggaatg ggatccggca atctggctgg
    12241 aagaactggc tacccttccc gatgccaccc gcaaacgcgc acaggcgctg gtcgctaaaa
    12301 gcatcaccat tgagttgttc tgtgcgccgg gtgaaattcc ctctgcccgc ttaccgatga
    12361 gcgatgtgcg tttttattcc cgcagcagta ttcgtttcgc ccgttgtgat tgtattgaag
    12421 gcacactttg cgaacatgtc gtactggcag tacaggcctt tgtccaggca aaagcgcagc
    12481 aagcggaatt gactcattta atctggcaga tgcgcagcga acacgtcaca tcatctgacg
    12541 atccgtttgc cagcgaagaa ggcaaaacgt gtcgtcaata tgttcaacaa ctaagccagg
    12601 cattatggct gagcggcatc agccagccgc ttattcacta cgaggccgct ttcagccgcg
    12661 cgcagcaagc agcggaacgc tgcagctggc gatgggtgag tgaatcgcta cggcaactgc
    12721 gcgccagcgt tgatgccttc cacgcccgcg ccagccacta tcatgccgga gaatgcttac
    12781 gtcagcttgc ggcattaaac agtcgattaa attgcgcaca agagatggcc cggagcgaca
    12841 gtgttggtga agttcctcct gtgccgtggc gcacggtcgt tggctctggc attgccggag
    12901 aagcaaagct tgatcatctg cggctggtgt ctttaggtat gcgttgctgg caggatattg
    12961 agcattatgg tttacgcatc tggtttaccg atcccgacac cggcagtatt ttgcaccttt
    13021 cgcgcagttg gccgcgaagt gaacaggaag actcaccggc agctacgcgt cggctgttta
    13081 gttttcaggc tggcgcactg gcgggtgggc aaattgtttc acaagcagca aaacgcagtg
    13141 ccgatggcga gctgctgtta gctacccgca accgcttaag cagcgttgta ccgctgtcgc
    13201 ctgatgcctg gcaaatgttg agcgcgccgt tacgccagcc gggcattgtg gctttgcggg
    13261 aatattta
//
LOCUS       DAJVPP010000103        13077 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVPP010000103 DAJVPP010000000
VERSION     DAJVPP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.103"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            55..1308
                     /gene="kch"
                     /locus_tag="OQM34_003193"
     CDS             55..1308
                     /gene="kch"
                     /locus_tag="OQM34_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1453530.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1363..1536)
                     /locus_tag="OQM34_003194"
     CDS             complement(1363..1536)
                     /locus_tag="OQM34_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1453531.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1679..3139
                     /gene="cls"
                     /locus_tag="OQM34_003195"
     CDS             1679..3139
                     /gene="cls"
                     /locus_tag="OQM34_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1453532.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALSRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3174..3503
                     /locus_tag="OQM34_003196"
     CDS             3174..3503
                     /locus_tag="OQM34_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1453533.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3556..4560)
                     /gene="oppF"
                     /locus_tag="OQM34_003197"
     CDS             complement(3556..4560)
                     /gene="oppF"
                     /locus_tag="OQM34_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1453534.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4557..5570)
                     /gene="oppD"
                     /locus_tag="OQM34_003198"
     CDS             complement(4557..5570)
                     /gene="oppD"
                     /locus_tag="OQM34_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1453535.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5582..6490)
                     /gene="oppC"
                     /locus_tag="OQM34_003199"
     CDS             complement(5582..6490)
                     /gene="oppC"
                     /locus_tag="OQM34_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1453536.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6505..7425)
                     /gene="oppB"
                     /locus_tag="OQM34_003200"
     CDS             complement(6505..7425)
                     /gene="oppB"
                     /locus_tag="OQM34_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1453537.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7511..9142)
                     /gene="oppA"
                     /locus_tag="OQM34_003201"
     CDS             complement(7511..9142)
                     /gene="oppA"
                     /locus_tag="OQM34_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1453538.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(9880..10527)
                     /gene="ychE"
                     /locus_tag="OQM34_003202"
     CDS             complement(9880..10527)
                     /gene="ychE"
                     /locus_tag="OQM34_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1453539.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11004..>13077
                     /gene="adhE"
                     /locus_tag="OQM34_003203"
                     /gene_synonym="adhC"
     CDS             11004..>13077
                     /gene="adhE"
                     /locus_tag="OQM34_003203"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS1453540.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYH"
BASE COUNT         3428 a         3233 c         3039 g         3377 t
ORIGIN      
        1 cagaaaatgt gcacgctaag ataattaaac aaaattgtgg ttacagaggt atcagtgagt
       61 cactgggcta cattcaaaca aacagcaaca aatttatggg tgaccctacg gcacgacatt
      121 ctcgcgctgg ccgtcttttt aaatggattg cttattttta aaacaatcta tggtatgtcg
      181 gtcaatttgc ttgatatttt ccatatcaaa gcattttcag agttggatct ctccttgctg
      241 gcaaacgccc cactatttat gctcggcgtc tttcttgtcc tgaactccat tggcttactg
      301 ttccgggcaa agctcgcatg ggcaatcagt atcattttgt tgttgatagc gctaatttac
      361 accctgcatt tttatccctg gctgaaattt agtattggat tttgcatttt tacgctggtg
      421 tttttgctga tactgcgcaa agacttctcc cacagtagcg ccgcagccgg gacaattttt
      481 gcatttatta gtttcacgac attactgttt tactcaacct acggtgcgct ttatttaagc
      541 gaaggtttta atccgcgaat agaaagtttg atgaccgcgt tctatttttc gatagaaacc
      601 atgtcaaccg tcggctatgg agatattgtc cctgtttctg aatcagcacg attgttcact
      661 atttcggtca ttatttccgg cattaccgtt tttgccacat ctatgacctc aatttttggt
      721 ccgcttatcc gcgggggatt caacaaactt ttaaaaggaa acaatcatac aatgcatcgt
      781 aaagatcatt ttattgtttg cggacattcg attctcgcca tcaatacgat cctgcaactg
      841 aatcaacgcg gacaaaacgt aacggttatc agcaacttgc ctgaagatga tatcaagcaa
      901 cttgagcaac gcttaggcga taacgctgat gttatccccg gtgacagtaa tgacagttca
      961 gtattaaaga aagcgggaat cgatcgttgc cgggccattc tggcgctgag tgataacgat
     1021 gcagataacg cgtttgttgt actctcggca aaagatatga gcagtgatgt caaaacagtt
     1081 ctcgccgtca gtgatagcaa aaacctgaat aagattaaga tggtacatcc ggatatcatt
     1141 ctctcaccgc aactgtttgg cagcgaaatt ctggcgcgag ttttaaatgg tgaagagatt
     1201 aataatgata tgctcgtttc aatgttgttg aactcaggtc atggtatttt cagcgataac
     1261 gatgaacaag aaacgaaagc tgacagtaaa gaatcggcgc aaaaatagag tttaaagacg
     1321 gaagagtaaa cttattgggc accgcaatgg tgcccagtac atttagatat tgtagatcgc
     1381 aaacaataac gagttacgct gtttgtttag aatgcacttc ctgatgctgt ggatacgcat
     1441 atttcggcgc gattgcccct caagccaacg cgatttacgt cggctagcct gacgctgcat
     1501 gcgccagcgc cccacttccg ttctactccg cttcatgtta actactctat gcaataacag
     1561 aatggtcatt atacccccgc ccccgacgct gaccagtgct tttcccgcgt ttttatttgc
     1621 cagatgaatc catatgcgta aactcataac aatgcgcttt caaaaggatt tctaatctat
     1681 gacaaccgtt tatacgttgg tgagttggtt ggccattctg ggatactggt tgctcattgc
     1741 aggcgtaact ttacgcattc taatgaaacg acgcgcagtt ccctccgcga tggcctggct
     1801 gttgattatt tacattctgc cgttagtcgg aattattgcc tatcttgccg ttggcgagct
     1861 ccatttaggc aaacgccgcg ctgagcgcgc cagagcgatg tggccttcca ccgcaaaatg
     1921 gcttaacgac cttaaagcct gtaagcatat cttcgccgaa gaaaatagca gtgtcgctgc
     1981 gccattattc aagctttgcg agcgtcgtca ggggatcgct ggggtcaaag ggaatcagct
     2041 acagctgatg accgagtcag atgatgtgat gcaggcgtta agccgcgaca tccagctcgc
     2101 gcgccataat attgagatgg tgttttatat ctggcagccc ggcggcatgg cggaccaggt
     2161 ggctgaatca ttaatggcgg ctgcacggcg tggtattcat tgccgattga tgctcgactc
     2221 cgccgggagt gtggcttttt tccgcagccc gtggcccgag ctaatgcgta atgccggtat
     2281 tgaagtggtc gaagccttaa aggtcaatct gatgcgtgtg tttttacgcc gtatggacct
     2341 gcgccaacat cgcaagatga tcatgatcga taattacatc gcgtacaccg gcagcatgaa
     2401 tatggtcgat cctcgctact tcaaacaaga tgcgggcgta gggcaatgga ttgatctgat
     2461 ggcgcgtatg gaaggcccca tcgccaccgc gatggggatt atttattcct gcgactggga
     2521 gattgaaacc ggaaaacgta ttctgccgcc accaccagat gtcaatatta tgccgtttga
     2581 acaggccagc ggtcacacca ttcacacaat tgcttctggc cccggctttc cggaagatct
     2641 cattcaccag gcattattga ctgcggctta ttcggcgcat gaatatttga tcatgaccac
     2701 gccctacttt gtgccaagcg atgatttact tcatgcgatt tgcacggcgg cgcagcgcgg
     2761 ggtggatgtc agtattatcc ttccgcgaaa aaatgactcg atgctggtcg gctgggccag
     2821 tcgcgcattc tttacggaac tgcttgctgc tggggttaaa atttatcagt ttgaaggcgg
     2881 gttactgcat accaagagcg tgctggtcga tggcgaacta agtctggttg gcaccgttaa
     2941 ccttgatatg cgtagtctgt ggctgaattt cgagattacc ttggcaatcg acgataaagg
     3001 ttttggtgca gaccttgccg ccgttcagga cgattatatt tcgcgttcac gtctgctcga
     3061 tgcccgttta tggctaaaac gtccattatg gcaacgtgtc gccgagcgac tgttttactt
     3121 cttcagcccg ttgctgtaaa acgtcgccaa cagaggttaa acaggtagtg actatggata
     3181 tggatctaaa caatcgcctg actgaagatg aaacgcttga gcaggcctac gatatttttc
     3241 tcgaactggc tgccgacaat ctcgatccag ctgatgttct gctgttcaat cttcagtttg
     3301 aagagcgcgg cggcgcggag ttattcgatc cggcggaaga ctggcaggaa catgttgatt
     3361 tcgatttgaa cccggacttt tttgccgaag tggtgattgg tctggcggac agtgaagatg
     3421 gtgaaatcaa cgatgttttc gcccgcattc tgttatgccg cgaaaaagat cacaaacttt
     3481 gccatatcat ctggcgggaa taaataatcg cttttagcgt gaaaaagggc tgacaactgt
     3541 cagcccttat tgtttttaaa gcggatcgac tttcaggcaa gaaacggcgt gtctgaaact
     3601 cccctccaga acaggacgtg ttttggcgca ctccggaccg gcaatcgggc aacgggtacg
     3661 gaaaacacaa ccggaaggcg ggttgatcgg cgacggtaat tccccttcca gtaactggat
     3721 ggttttgttc ttctccagat ccggatcagg tatggggact gccgacatca atgccctggt
     3781 gtaaggatgt agtggattgt ggtagacctc atcataggtc cccagttcta ctgcatggcc
     3841 gagatacatc accaacacac gatcggaaat gtgttttacc acggccaggt catgagcaat
     3901 aaaaattaat gacaatccca tctcacgttg cagctgctgg agcaggttaa ccacctgcgc
     3961 ctgaattgac acgtccagcg ccgaaaccgg ctcatcgcag ataatcagct tcggttcaag
     4021 aataagagca cgagcaatcc cgatacgctg gcactgccca ccggagaact catgcggata
     4081 gcggttaatc aggttaggca ataacccgac tttcagcatc atcgccttca cgcgctcgcg
     4141 aacttcctgg cgtgacattt tcggatgata agtacgcagt ggttcagcga tgatctcgcc
     4201 gatggtcata cgcgggttta gcgatgccaa cggatcctgg aaaatcatct gaatatcact
     4261 gcgaacggca cgccattcat cgggcttcat gcccagcaac tctttaccta accaggcaac
     4321 atgaccgtcg gtcgccttga ccaaaccgat gatggcgcga gcaaaggtgg acttaccgca
     4381 tcccgattcc cctaccacac ctaatgtttc cccttcatac aggcgaagag tgacaccatc
     4441 gacggctttg agcgttttcg gcggttgcca gaaccactgt ttgccatctt tgatttcaaa
     4501 gtgcacttta agatcggcga tttcaaggag gacttttctt ccttcagtta cagcattcat
     4561 aacagttcct ccaccggttt aaagcaagca cgcagacggc caggcgtaaa ctcttccagc
     4621 ggcggtgcgc tactacaaat ttccatcgca tgtggacaac gtggctggaa cgggcaacct
     4681 ttcggtaatc gcagcaagtt tggcggatta cccgggatgg tcaacattgt ttcaccttcc
     4741 gcatcaagac gcggcaccgc gttgagcaaa ccgatagaat aaggatgaac gggttgataa
     4801 aagacatcgc gagcattgcc atattccatc gtgcgcccgg cgtacattac cagcactttg
     4861 tcacagatcc ccgccaccac gccaagatcg tgggtgatca taatgatggc agtattaaat
     4921 tcccgcttca gttcattcaa tagcgtcatg atctgcgcct gtacggtgac gtccagcgct
     4981 gtagtgggtt catccgcaat cagcaactta ggtcgacata gcaatgccat cgcaatcatg
     5041 actcgctgac gcatgccgcc agaaaattcg tgcgggtaca ttttcatgcg tttacgtgct
     5101 tccggcattt ttaccgcatc gagcatccgc accgactctt caaacgcctc agctttgctc
     5161 atgttcttat gcagcatcag cacttccatc aactgctcac cgacgcgcat atagggattc
     5221 aacgaagtca ttgggtcctg aaaaatcatt gagatttgtt cagcgcgcag tttattgagt
     5281 tcacgctctg gcaaattgag gatttcacgc ccattgaagg ttgccgatcc gccaatgcgt
     5341 ccgttggcag ccaacaggcc catcaacgca aatgcagttt gcgatttacc cgaaccagac
     5401 tcacctacaa tgcccagcgt ttctccggca cgtagggaaa aatttaaatc attgaccgcc
     5461 gtgacgtcgc cgtccggggt actaaaggtg acacgcaaat ctttcacgtt cagcagtgcg
     5521 tcagcctgtt gttgtgcgag cggcacagtt gcagtttcaa ttacgctcat ggctgcactc
     5581 cttaacgatc tttcgggtcg agggcatcac gcaagccatc gccgataaag ttgaaacaaa
     5641 acagtgtcac cacaaggaat cccgctggga acaacagtaa ccatggagag acttccatcg
     5701 agttcgcgcc atcactcagc aatgcccccc agctgcttaa cggctcttgc gtacccaacc
     5761 ccaggaagct aaggaaagat tcaaagagga tcatgctggg caccagtagc gatgcgtaga
     5821 ccaccaccac accgagtacg ttcggcacaa tgtggcgaat aacaatgccc ggcgtcgata
     5881 caccgccaac ttgtgccgcc tcaataaact ctttgcgctt cagactcagg gtttgcccac
     5941 gcacaatacg agccatatcc agccaggaaa ccatgccaat cgccacgaaa atcagcagga
     6001 tgttttggcc gaaaaaggtc accagcaaaa tgacgaagaa catgaatggg aaggagttga
     6061 ggatttccag cagacgcatc attaccgaat ccactttacc gcccagataa ccggaaagcg
     6121 aaccataaag tgtccccacg accactgcca ccagtgccgc agcaacaccg accatgagtg
     6181 agatacgccc gccaatcgca acgcgcacaa gcaggtcgcg accggatgag tcagtaccaa
     6241 agtagtgacc ggactccata tccggggcgc tggacatcat cgcccagtca gtatcgtcat
     6301 aggcaaactg ggaaagcatc ggtgccagga ttacaaataa cgcgatcagc accagcacta
     6361 tcagactggc aaccgccgca cggttatgca taaaacgtcg acgtgcgtcc tgccacaagc
     6421 tgcgcccttc gacctccagc ttttcactga aattttccag cgtctcgctg tttttcttac
     6481 ttaacatcat cgcgagctcc agtatcagta acggattttc gggtcgataa ccgcatatag
     6541 cacatcgaca atggcattaa acaaaatggt taacgcacca accaggatgg tcaggcttaa
     6601 cactaaggaa tagtcacggt tcaatgcgcc attaacgaac agttgcccaa tccccggcaa
     6661 accataaatg gtttcgatga ccatagaacc ggtaataatg ccgacaaatg cggggcccat
     6721 ataggagagc acgggtaaca gagcaggttt taatgcgtgg cgtaaaatga tccgccgcat
     6781 aggtaaccct ttcgcccggg cagtacgaat aaagttggag tgtaatactt caatcataga
     6841 gccacgggta atacgcgcaa tactggcgat ataggcgagt gacaacgcca ccatcggcaa
     6901 tatcatgaat ttaagcgccc caccattcca gccaccgccc ggcagccaat gcaaaatgat
     6961 cgcaaatatc atgactaata atggcgcaac cacaaaactg gggataacaa ccccggtcat
     7021 tgccagcccc atcacggtat agtcccattt ggtgttttgt tttaatgcgg caataacgcc
     7081 agcactaaca cccagtatta ccgcaaggaa aaatgcggcg gctcccagtt tggcagaaac
     7141 gggaaaactg gatgccacca ggtcattgac cgaataatct ttatatttaa acgatggacc
     7201 gaaatcgcca tgcgccagtt gtttcaggta gctgaaatac tgtgtcatga tcggatcatt
     7261 aagatgatat ttcgcttcga tattggccat cacttctggc ggtaaagtac gttcgccggt
     7321 aaaagggctt cccggcgcga ggcgcatcat aaagaacgaa atagtaataa gaataaatag
     7381 cgtcggaatc gcttccagac aacgacgtag aataaatttt aacattgccc taccttctgg
     7441 cctgtgcctt ctgtaatgcg ataaaaatca gacaccgtgg agcaggacac tcctgcccca
     7501 cgtattgcca ttagtgcttc acaatgtaca tattccgggt ataggtatta tccagcggat
     7561 ctttgccggt atagccacca acccacggtt tcaccagacg cgcattcacg tagtaataaa
     7621 caggaacaat ggccgaatcc ttatccagct gttgttctgc tttagtgtac agagctgtgc
     7681 gctgcgcctc gtcagtcact ttcagcgttt ccgccataat gctgtcaaag gccgggctct
     7741 tataatgcgc ggtattcatc gagctgttcg acagcatggt gttcaggaag gaagttggtt
     7801 cgttgtagtc tgcacaccag cctgcacggg ccacatcaaa agtgccctgg tgacgggtgt
     7861 cgaggaacgt tttccactcc tggttaacca gtttgacgtt tacaccaatg tttttcttcc
     7921 acaatgaaga ggcagcaatc gccagctttt tatgcagatc ggaggtgtta taccgcaggt
     7981 tgatggtcaa cggtttgtct gcggtataac ccgcttcagc cagcagtttt ttcgcttctt
     8041 cgttacgttt ttcctggctc cagccaaacc attctggctg agtcaatttt gcgccatcag
     8101 tatacggtgg agtataacca taggcgggca tgtcgccctg cgctttcact ttattaacaa
     8161 tgatatcgcg gtccatacct agtttcagtg cggtacgcac acgcacatcg ttgaatggcg
     8221 gtttctggtt gttaatttcg taatagtaag tgcacaggta tggatcaacg tgaacttcgt
     8281 ccgggatctc ttttttcagc ttctggaaca attcgatcgg catgttgtta tacgtcatgt
     8341 cgatttcacc actacggtag cggttgacat cggtaacttc agaagcaata ggcaaatagg
     8401 ttacctggtt aataacggtt ttcgcgttgt tccagtaggt cgggctgcgt tcaagaacga
     8461 ttcgttcgtt tacgacccaa tcttttaagg tataggcacc gttggtgacg atattaccag
     8521 gctgggtcca tttttcgccg aatttctcga tagcggcttt tggcaccggt gaagttgatg
     8581 ggtgaacaag taatttatag aagtacggaa cgggttcact taaggtgact tctaatgtgt
     8641 gatcatcaat agctttcacg ccgagatcgg taatcggttt tttcccttca agaatttcat
     8701 caataccggc gatatgccca tattgcagat aactggcata cggagaagca gtgttcggat
     8761 caacagaacg ttgccagcta tacacaaagt cttgtgctgt gactggcgtg ccatcagacc
     8821 atttcgcatc tttacgcaaa tggaaggtcc agactttcgc gtctttatta tcccaggatt
     8881 cagcgacgcc aggtgctgga tgaccgtcaa gatcgctgac cagtaagcct tcaaacaggt
     8941 ctcggctgat attagactcc ggaacacctt caattttgtg cggatctaat gactgaactt
     9001 ctgaaccatt gttacgtacc agtgtttgtt tttccgccag tgtgacgcct gcgggtacat
     9061 cagctgccag cgcgacattc cctgccatta gcgcagccag aacgccagct gctactaaac
     9121 ttctcttggt gatgttggtc attgtttttt ggactccctc attataatta ctggtattaa
     9181 ccagcatgtg taatcccgga ttgggaccct gcacaagctc ggagactttc tgctgtcagg
     9241 ttactttatc gttacgtatt actatcaccg actttattta ttctggtgat tcagacgatc
     9301 accttatgtc gattcgtact gtgtctcccc tgtcgagaca cattattctt tcgcgacgct
     9361 ctttaattgt taattattct cattaccaat aaagaatcgt ctggcggttg cccggaaagt
     9421 accaaatgcg gttcttactc gccaatacat tttgcaaaat tgttaagcga ttctctttta
     9481 ttgaccgtat cagaatctat agcttgcatc catgccttaa caaacatctt tcaattcaat
     9541 caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt agtgaaatgc
     9601 acaataattt aacaattgat tattatttag cactttcctc taccaaaggc agtggaatta
     9661 ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg aaaataacat
     9721 gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg agaagaatat
     9781 ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa taatgacgaa
     9841 gtaaacgggg catggcagct ccgtttcatt tgaaaggaat taattaagca ggccggggaa
     9901 aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca atagccccat
     9961 aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc acggtgccat
    10021 gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga aaccaaacag
    10081 atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc
    10141 cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg cggtttctga
    10201 tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg acatcgctat
    10261 cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac caaaaagttg
    10321 tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag acaggttggc
    10381 tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac
    10441 gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac
    10501 gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg gataatgtta
    10561 gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct
    10621 cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa
    10681 tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg aaaagcgatg
    10741 ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta cccagaagtg
    10801 agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa
    10861 actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt tcatcggtta
    10921 agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag
    10981 tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg
    11041 tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca
    11101 aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg
    11161 ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt
    11221 ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg
    11281 acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt atcgttccga
    11341 ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag acccgtaacg
    11401 ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg gctgatatcg
    11461 ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac
    11521 cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga
    11581 ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct atcggtgtag
    11641 gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt gcagttgcat
    11701 ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg
    11761 ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac ggcggctatc
    11821 tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat ggtgcgctga
    11881 acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc ttctctgtac
    11941 cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc gaaccgttcg
    12001 cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc gaagacgcgg
    12061 tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct tgcctgtaca
    12121 ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacagctc
    12181 gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat aacttcaaac
    12241 tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc tctgaaaacg
    12301 ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt
    12361 ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg
    12421 aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca
    12481 atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa actgaagtct
    12541 tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa
    12601 actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgccgcga
    12661 agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg gcgctgcgct
    12721 ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa gcgaaaatga
    12781 tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg
    12841 acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg gatatggcga
    12901 ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg
    12961 acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg
    13021 gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc taccacg
//
LOCUS       DAJVPP010000104        13068 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVPP010000104 DAJVPP010000000
VERSION     DAJVPP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.104"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..606)
                     /locus_tag="OQM34_003204"
     CDS             complement(<1..606)
                     /locus_tag="OQM34_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1453541.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTW"
     gene            847..1647
                     /locus_tag="OQM34_003205"
     CDS             847..1647
                     /locus_tag="OQM34_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1453542.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1725..2495
                     /locus_tag="OQM34_003206"
     CDS             1725..2495
                     /locus_tag="OQM34_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1453543.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2543..3901)
                     /gene="mltD"
                     /locus_tag="OQM34_003207"
     CDS             complement(2543..3901)
                     /gene="mltD"
                     /locus_tag="OQM34_003207"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1453544.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(3973..4728)
                     /gene="gloB"
                     /locus_tag="OQM34_003208"
     CDS             complement(3973..4728)
                     /gene="gloB"
                     /locus_tag="OQM34_003208"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1453545.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            4762..5484
                     /locus_tag="OQM34_003209"
     CDS             4762..5484
                     /locus_tag="OQM34_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1453546.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5481..5948)
                     /gene="rnhA"
                     /locus_tag="OQM34_003210"
     CDS             complement(5481..5948)
                     /gene="rnhA"
                     /locus_tag="OQM34_003210"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1453547.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6013..6744
                     /gene="dnaQ"
                     /locus_tag="OQM34_003211"
     CDS             6013..6744
                     /gene="dnaQ"
                     /locus_tag="OQM34_003211"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1453548.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            6877..6953
                     /locus_tag="OQM34_003212"
     tRNA            6877..6953
                     /locus_tag="OQM34_003212"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6911..6913,aa:Asp,seq:gtc)
     gene            complement(7084..8130)
                     /locus_tag="OQM34_003213"
     CDS             complement(7084..8130)
                     /locus_tag="OQM34_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCS1453549.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIHDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8141..9126)
                     /gene="tssG"
                     /locus_tag="OQM34_003214"
                     /pseudo
     CDS             complement(8141..9126)
                     /gene="tssG"
                     /locus_tag="OQM34_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9123..11006)
                     /gene="tssF"
                     /locus_tag="OQM34_003215"
     CDS             complement(9123..11006)
                     /gene="tssF"
                     /locus_tag="OQM34_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCS1453550.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEDESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11022..11516)
                     /gene="tssE"
                     /locus_tag="OQM34_003216"
     CDS             complement(11022..11516)
                     /gene="tssE"
                     /locus_tag="OQM34_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCS1453551.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11513..12343)
                     /locus_tag="OQM34_003217"
                     /pseudo
     CDS             complement(11513..12343)
                     /locus_tag="OQM34_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12330..13068)
                     /gene="tagK"
                     /locus_tag="OQM34_003218"
                     /pseudo
     CDS             complement(12330..13068)
                     /gene="tagK"
                     /locus_tag="OQM34_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         2990 a         3406 c         3506 g         3166 t
ORIGIN      
        1 ccaggtattg agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc
       61 gatcgtttct ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata
      121 actgttaaat aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc
      181 gacatccact tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt
      241 gacttccgga tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac
      301 tggagttgcg gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct ccataatttc
      361 tgattctgcc gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc
      421 ttcttccgtc aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat
      481 ttccagcttt ttcaccgccc ggcttaccgc tgagtttgct tgccctaatt gttccgctgc
      541 ccggctaaag ctgccgcttt cgacgaccga aacaaaaatg gcgagttctt ccgacgtggc
      601 tttcattttt gctcctgttg caaaatagat gagatatttt gaatttattt gtcattaaac
      661 catcaggatg cgtgatatgt catccgattt aatgttctca ataatgagca aatttctgac
      721 aggtgtaagc acttgcttac ataacaatat acaattgctc gttgaaagag tgagctaaaa
      781 tccctataac agtagaaccc tcccgagtgc ggaagggttg acgtaataga ggtttcaaag
      841 tcaaaagtgc gaaaaaacac ctatgccatg cgctatgttg ccggacaacc tgcggaaagg
      901 atcttaccgc cggggtcttt tgcgagcatc ggccaggcat taccacctgg agaaccgtta
      961 agtaccgaag agcgtattcg gatcctggtg tggaacatat acaaacagca acgcgctgaa
     1021 tggttgtcgg tattaaagaa ctacggcaaa gatgcacatc tggtgttatt gcaggaagcg
     1081 cagacaacgc cagagttagt acagtttgcg accgctaact atcttgccgc cgatcaggtg
     1141 cccgctttcg tgctgccaca acatccttcc ggtgtaatga ccctttcggc ggcgcatcca
     1201 gtgtattgct gcccgttacg tgaacgagaa cccattttgc gtctggcgaa gtcggcactg
     1261 gtgacggtct atccattacc tgacacccgc ctgttgatgg tggttaatat acacgccgtc
     1321 aacttcagtc tgggcgtgga tgtctatagt aagcagttac ttcctattgg tgatcagata
     1381 gctcaccaca gcggcccggt cattatggcg ggagatttca atgcctggag ccgtagaagg
     1441 atgaacgcgt tatatcgctt tgcgcgggaa atgtcgctgc gccaggtgcg ttttaccgat
     1501 gatcagcgcc gtcgggcgtt tggccgcccg ctcgattttg ttttctaccg tggtctgaac
     1561 gtcagcgaag cttctgtatt ggttacgcgc gcttccgatc acaatccgct actcgttgaa
     1621 ttcagtcccg gcaagcctga taaataaggt atgtcaggtc tgccacaggg cagaccaacg
     1681 tttggcgctg tccttttata ctcaaacaac aaaaggacag cgcaatgaca acacaatccc
     1741 accatgacca cgtagaaaag cagtttagct ctcaagcctg cgaatatctg accagtaccg
     1801 tacatgcatc cggacgagat ttacagcgcc tggcggtgcg tctggctgat tatcctggtg
     1861 caagtgcact tgatatgggc tgcggagcag ggcatgccag ctttgttgct gcgcaaaacg
     1921 tgagcgcggt ggtggcgtat gacttatctg cccaaatgct ggatgtcgtg gcacaagctg
     1981 ccgaagcccg gcaactgaaa aatatcacca cccgccaggg atatgccgaa agtctgccat
     2041 ttgccgataa cgcatttgat attgttatca gccgttattc tgcccatcac tggcatgatg
     2101 ttggtgcagc actgcgtgaa gtgaaccgga tattgaaacc tggcggtagg atgattgtga
     2161 tggacgtaat gtctccgggt catccagtgc gcgacatctg gttacagacg gtagaagcat
     2221 tacgcgatac ctctcacgta cgaaactacg ccagcggtga gtggttgagg ttaatcaatg
     2281 aagccaatct gatagttgat aatttaatta cagataagtt accgctggaa ttttgttcat
     2341 gggtcgcgag aatgcgtacg ccagaagcgt tagtagacgc tattcgcatt taccaacaga
     2401 gcgcatcgac agaggtgaga acgtattttg ccttgcagaa tgacggctct ttcaccagtg
     2461 atatcatcat ggtagaagca cataaagcgg cataaataaa aaaggcaccg ggggaatcgg
     2521 taccttttta ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg
     2581 tcagcttgtc gcctggttgc agattcgcag tgtcgctgtt ccagcgcatc acatctttga
     2641 tgttcacgcc gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg
     2701 taatgctatc gctgttgttt gccagtcgct gtgcgctact gcctgcacca atcgtcaaac
     2761 tttggcctgg cttcagctta gatccgcgca gtttgttcca ctgctgcaaa tctttggtgc
     2821 ttacgccgag acgtgaagcg atgcttgaaa gcgtgtcgcc agagcgtacg gtgtaaacac
     2881 ggctgttaag cggcgtattg ttggcaacca gcgtcgactg tacagcagca atttcgcctg
     2941 aagcgagaga ttcacgcagt tgatctgcat gcttctttgg caccatcacg tactgcggcc
     3001 cacttgcgcc cagcgtggag cctttcacgc cagcgttgaa tgtcttcagc ttgctgacgg
     3061 aaatccccgc catatctgca accttcgcca tttcaaccgg gctactcagg tgcacacgcg
     3121 ccagagcacg gctttcatcg gtcgttggca gacgtacgcc ataacgcttg ctgtttttga
     3181 gaatatcact caatgccagc attttaggca cgtactgctt cgtttcctgc ggcaacggta
     3241 acgaccagaa gtccgtggat ttcccacgcg ctttgttcgt tttaattgcc ttcatgaccc
     3301 gaccttcgcc gctgttataa gccgctacgg tcagaagcca gtcgtcgtca aacattttgt
     3361 tcagacgctg catcatattc agcgcggcag ttgttgaagc aacaacatcg cgacgcgcgt
     3421 cataattgcg ggtctgtttc aaaccataat tgcgccccgt gctcggaatg atctgccaga
     3481 tgcctgcggc attggcgcca gacgttgcgt gaggatcaaa agcgctctcc actatgggta
     3541 gtagtaccag ttccataggc atgttacgtt ttttaacttg ccctgctatc cagtacatat
     3601 acggctctgc ccgtaaagtt acatcgtgga gatagctctt attgcgtaaa tatttctgtt
     3661 tctgttcgcg aatccggtca ttttccggaa ttcccatctt tagctcgtcg ccaatgaaag
     3721 cccacaagtc accatctggc gcgatagacg tcccatcgtc catccatcgt gcctgacttg
     3781 taaactttgc tgcttcccct tgaccagctg cagaaaggct ctgtgcgtgc tgttgaacgt
     3841 tgccggtact ctggcaaccc acgagcagga cagaggcgag taatatcgct tttgccttca
     3901 tgtgtgtgtc aatagttgct taaaagacga ccgatcataa cggcgaacgg agccgatgac
     3961 aagaaagttt tatcagaacc tatctttctt tgaccttaac catgcaaaac gctcttcagg
     4021 ttgttgcaat aatgtttctt cattaattcc attaattaaa tcaatatctt ccgttcttaa
     4081 aaaaacatta atttgccgct catttttcag aattacgggt agtgttattt gattttttgc
     4141 ccgtaactcc ttaactttac gataataatc atttatggac aaatcgtgcg gaaaaatact
     4201 caaagcaaac ttcatatttg ataacgtata ttcatgagca caacatacca atgtatcgtc
     4261 aggtaacgca cttagctttt taagtgattg atacatttgt aaagctgtcc cttcaaacaa
     4321 ccgaccacaa ccaccagaaa acagcgtgtc gccgcaaaat agataaggtt tactgaagta
     4381 acagatatgt cctaaagtgt gacccggtgt ggtaattaca ctaaattcat gccctaaaac
     4441 gaaggcagtt tcgccatctt tgactacctg tgttgttccc ttatcttgtg tctcttgtgg
     4501 accatacacc acaatttgcg gaaacttttc caccagttct tttacgccgc caacgtgatc
     4561 gtggtgatgg tgggttagaa atatggcctc cggttgccag ttattggcgg taatggcgtt
     4621 taatactggc tctgcgtctc cgggatcgac aatcaggcag cgacctgcct catcattcaa
     4681 aacccagatg taattgtcat caaaggcggg aatactgtta agattcatag attacctctc
     4741 agtgtaaaac ggaaggttgt gatgaaaccg gcaagagtcc ctcaaactgt cgtggctcct
     4801 gattgctggg gcgatttgcc ctggggagag ctttatcgca aggcgctgga gcgccagctc
     4861 aacccgtggt tcactaaaat gtatggtttt catctgctta agattggcaa tttaagcgca
     4921 gaaatcaatt gcgaagcgtg cgcggtttct catcaagtga atgtttctgc gcaaggaatg
     4981 cccgtccagg tgcaggcgga tccacttcat cttccttttg ccgataaatc cgttgatatt
     5041 tgtctactgg cacatacctt gccgtggtgc accgatccgc atcgtttatt acgtgaagcc
     5101 gatcgggtac tgattgatga tggctggctg gtgattagtg gcttcaaccc catcagttta
     5161 atgggattac gcaaacttgt gccggtattg cgcaaaacct cgccctataa tagccggatg
     5221 tttactctga tgcggcaact ggactggctc tctttgttga attttgaagt gctgcacgcc
     5281 agccgtttcc acgttctccc gtggaacaaa cacggaggaa aactattgaa tgcgcatatt
     5341 cctgcgcttg gttgcttaca acttattgtt gcccggaaac ggactattcc tttaacgcta
     5401 aatccgatga aacagagtaa aaacaagcca cgaattcgcc aggcggttgg agccacccgg
     5461 caatgtcgta aaccacaggc ttaaacttcg acttggtagc ccgtatcttc cagtgtggga
     5521 ttcatcgccg cggcacgagc cagttcatca cagcgttcgt tttccgggtg tccggcatgg
     5581 cctttaaccc attcccattt gatttgatgc tgccccaatg cagcatcaag acgttgccag
     5641 agatcgacat tttttactgg ttttttgtct gctgttttcc agccacgttt tttccagtta
     5701 tggatccact gggtgatacc ctggcggaca tactggctgt cggtactcaa aatgacttcg
     5761 caatgttctt ttaacgcctc cagcgcgaca atagcggcca tcaactccat acggttgttg
     5821 gtggtgcggg tgtagcctgc gctaaaggtt ttctcgcgtc cgcgatagcg taaaatagcg
     5881 ccgtaacccc caggtccagg attacccaga cacgaaccat cggtgaaaat ttctacctgt
     5941 ttaagcatct ctggtagact tcctgtaatt gaatcgaact gtaaaacgac aagtctgaca
     6001 taaatgaccg ctatgagcac tgcaattaca cgccagatcg ttctcgatac cgaaaccacc
     6061 ggtatgaacc agattggtgc acactatgaa ggccacaaga tcattgagat tggtgctgtt
     6121 gaagtagtga accgtcgcct gacgggcaat aacttccatg tttatctcaa acccgatcgg
     6181 ctggtggatc cggaagcctt tggcgtacat ggtattgctg atgaattttt gctcgataag
     6241 cccacgtttg ccgaagtagc cgatgagttt atggactata tccgcggcgc ggagttggtg
     6301 atccataacg cagcgttcga tataggcttt atggactacg agttttcgtt gcttaagcgc
     6361 gatattccga agaccaatac cttctgtaag gtcaccgata gccttgcggt ggcgaggaaa
     6421 atgtttcccg gtaaacgcaa cagcctcgat gcgctatgtg cccgctacga aatagataac
     6481 agtaaacgaa cgctgcacgg ggcattactc gatgcccaga tccttgcgga agtttatctg
     6541 gcgatgaccg gtggtcaaac gtcgatggct tttgcgatgg aaggagagac acagcagcaa
     6601 caaggtgaag caacaattca gcgcattgta cgtcaggcaa gtaagttacg cgttgttttt
     6661 gcgacagatg aagagctggc agcacatgaa gcccgtctcg atctggtgca gaagaaaggc
     6721 ggaagctgcc tctggcgggc ataaatacat gtaaaaggcg ctaaaaatag cgacttgggc
     6781 gatttttgca gcaaacgatt caaaagatga gaaaaatcgt tgacgaaggt cgaggcaatc
     6841 cgtaatattc gcctcgttcc caacggaaca caacgcggag cggtagttca gtcggttaga
     6901 atacctgcct gtcacgcagg gggtcgcggg ttcgagtccc gtccgttccg ccaatattca
     6961 gaaagcccga atcagtaatg attcgggctt tcgtcgtttt ggtgaagtga gaatttttca
     7021 ctccgaagta aaacggatat gatttaaaaa atggccagac attataaatt ctggccactt
     7081 ctgttactcc agcgcatccc atttctccag cagttctact ggaatttcat ttattacctc
     7141 cgagacgcgt tttccgacca ggcgttctgc ctggcgcagc aaaggaatgg tcgggctgct
     7201 cggttcgctg tgctcaaacc agcgacggat gacacgcagg cgttccagcg cgtcgtttcg
     7261 gtcgtggatc tgccggggtt ctgcgccggt catcggtgat atttcctccg cgatatgcac
     7321 cggtagcgca atctgctctg cctgcatctg ttcagtatga acgatggtgg cgttttccgg
     7381 ctgctcagcc tgtggttgtg gcaggatctc cggcgtggtg ctctgcactg ctcccggtaa
     7441 cagggccagc agttgcgtca gacgggagaa gtccggtgcc tggtcattca gcgtttcacg
     7501 cgcatggcgt tgcagccgct cagtggtctc tgctgcctgg cgaaaggcat ccagcggcag
     7561 tgtgccgcgc gcttccaggt ctgccagttg ctggcgtacc gattctggtg ccagcgcatc
     7621 cgccgggcgt aatgcagata gcgaacgctc cacatcccgc acctgaaggc gcatggcagc
     7681 attatttgac agtgtaatgc cacgaatatc cgccattaca ccttcgtggt ccagcaacgc
     7741 cgccagcgcg ttactgcgtg ccaccgcagc atcttcggcg gtgatatctt cagtcgcgag
     7801 cagttgagga tggatggcgt ccgggtacgt ggagcacaac gtttccagca gcgtgagcgc
     7861 ctccgacagt cccctggcac cggcctgctg gatacggcag cgcagcagga caaccagaat
     7921 gcggatatct ttgctgcgca tcagcagacg atgggcgtcc cgctcaagct ccgcccagtt
     7981 aatgttttcc ggcgtactga caaaatcgcc atattgcgct tcttcacgcg gtgcggcgcg
     8041 ggtgaacagc agcaggtatt ccggatcgta ctccagatcc gggccgcagg ggcattccgc
     8101 cgaaattggg tcagacagga atatttccat ttaattatcc tcagtgcgcc aggtactgtt
     8161 ctggttcgaa aatcatgccc gtcaccgggt tgtcgtgcat ggctttaccc gcccatgcag
     8221 tccaacccag ttggtacgga ccaccggtaa cagccggagg ggcggcgtgg ggctttagct
     8281 gtagctcaat ttcccagcag tattcataac cactaaaggc gcggaccagt tctgtcagga
     8341 cgggcaggtt attgctgcca ggcagaaaac gtcggtaatc ctccagcgtc agcgggccga
     8401 taaccagccg gaacttatac tgcatgtccg gcacggcctg gccgatgagt gcaccattgc
     8461 caataacgga agagggggca ggcatcccca gccgggtgat ctcatcgtcc gccacggcta
     8521 tccagtgcag gacatactcc tgtatccgga aaggtacacc gaaatagtgc gccagcgtgg
     8581 tggccagacc gtccggattg cgggactcac gcaccagatg ggccgatgcc gccagacgag
     8641 cgtgatctgg caacgggctg tcagcgctct cccgcaaatc ctgcccgctg aggctggcga
     8701 cataaaaggc aaagcggtca tgttgcggtt tatccagacc gcccccagcg gactgggcgc
     8761 tgcgccatgc ctggtaaaac tgcgtcagcc agcggtgatg aaaaagatca gaaaaacggg
     8821 tcagcgtcgg ctttcagtcc ggttacgcgc cagttcggtg tagtgcagcg gcagtggacc
     8881 gttcggcccc cacagcccga ggctgtacag actgatgtgc aggcgccctt cctgccagcc
     8941 cacactggcg atttctcgtg gcgcaaaggt cagcgcgggc gtttgtccga ggcgaaattt
     9001 ttccatccgt ggttgccagg tgttgcccgc cggaacggta cacaactgca catccacgcg
     9061 gcgcatcagg ttcagaaaac cgtaacgcca ggggggggcc tgcgcctgag tgaaatcgtt
     9121 gctcatacgc ttccccgctg tcccgccctg acaggccagg tcataatttt gccgcgctgc
     9181 atgctgtgca gcgtcatctg cgagaaggta ttaatggaaa catggcgggc gatatagtgt
     9241 tccagcacca ggccgaacag atacggactg atgccggaaa aaccttcttc atccaccgtc
     9301 agttcacaac tgacgccacg cccatagacc agtagtccgg aaccgggcag acggcgggtg
     9361 accggggtcg ttttacaacc aatcaggctg cgcacctggc gcgattgcgg actgtcatga
     9421 gcaggaataa acaggttcag cagatctcgc aacgcctggc cgccggtacg gtgatccaga
     9481 tcggccagcg gcagataatt aaaagataac tggcggatca accgccatgc catctcacgt
     9541 tcagccagcg ggggctgcgg cgagcgtggc gggcggataa gccctacgcc cgccaccggg
     9601 atggctgcat ccacggttag atcgtcccgg ccattgcggg caataagaca gggcagatcg
     9661 cggttagtga ccatggccgt gatggtgatg tggcgcagat tttccgggta cggcgcttca
     9721 tactggtcaa ccagcgacag gaacacttcc gagcccgtat agggggtacg ggtgccgtaa
     9781 cggcgggcgt tttctgataa gcggcggggc tcccggcgca gggaaaaata acgtccgtga
     9841 ttgccttcat cattattgcg ggtgtggtag agcgggcgaa aggccatctt ccgtgtggtg
     9901 tcggtttcca gcccttccac ttcctggacc gagaacacct cataatccag cggatgggtt
     9961 ctgtccacca ccagatgctg ttccgtcgtg ctgtgcgtga catcgatccg ggcggtgacg
    10021 cggggaaaca ggttgataac cggggtacag aacaggctga actgtgccgc atttgtctgg
    10081 tggatcagcc agtccggcgg caggcggttc agcaggataa caatctccgc cacgccaccg
    10141 tcaattttct gtaatccggc agacaggcct gtcggggtga aaaaatagaa gcgttccgga
    10201 caggcaaaat attcatgcag caggttatga ccgtgaaaca cgttccacgc cagcggcagc
    10261 agcccctgat ccggctccag gccttcgtac ataacgggct gttgcagatt cacatccagc
    10321 gcgccgtcaa aatgccccgg aatgccagcc agggtcgcga tggcgctggt atgtagaagt
    10381 tctaacaggt gtgaggccgt acgctcctcg ccgcagagat aaaacggcag cctgtccagt
    10441 ccggcaagct gactgaacgt tagttcacca aaggtgcgca gggtaatacg cagcgccccg
    10501 gcgacgtgaa tgttggccgg caggtagcgg tgcagggcag gcatatccgg tggcgcggcg
    10561 gtcagtcgcg cctcttcgat ggcgagcggc cacagcgtta catcctggct gctgcgaaac
    10621 tggcaggcag tgctttcgtc ttccggaatg gcggagaaaa aggctgtgtc gcgcgggacg
    10681 gtaaatcctt tcgccagatc gccttcttcc gtatcgggat gcaactgcgc cacgctcatt
    10741 gagggcgtgg gtgtgacgta attggggctg accacttcca gcaggcgctg ggtgaaacgg
    10801 gggaattcag cgtcaatttt tagctgcgtg cgggcggtca ggaagctgaa ggcctcgacc
    10861 attcgctcta catacggatc ggcaatatcc gttccctgca tgcccagccg ggcggcgact
    10921 ttgggatgac gggcggcaaa ttcggctccc gtttcccgca gatagctcag ttcgcggttg
    10981 tagtattcca gtagccgtgg atccatcggt ctctctctga attaaaaaaa cgtaatgcga
    11041 ctctgctcca tatccagcgc gctacgtacc cggaatgcag tcggatacgg ctgcgtgagg
    11101 atctcgccgc gaatttcaaa ctgtaatgtg ttatggccac tctggcggcg ctcatcctga
    11161 agtgggatga tttgcagagt ggacgctttc agccggggtt caaaccgggt gatggttcgc
    11221 aaaatggccc tgcggatatc gtcccacttg tgctcataca gaaagctgcc cgccagtggc
    11281 ggcaggccgt agttaagtac ggacgctgcc gcgtacggat aacgtgcagc atcaatatca
    11341 tcttcatggc tgacggtgtt gagcagaaat gacaaatccc ggcggatgat ctccttaatc
    11401 tgaaccggac tgacgctgat accgtggtcg cgtttctggt ttggggcgtc gtcacacagg
    11461 cggtcaaaca gcgtgggcag caggtgattg cctggacgct ggcgggaagc gctcacgcgt
    11521 cgcttccttc agcggtgtta aaggtacagg tggacaaatc cagcaggctg atatcgccgt
    11581 gaccggtgag ccacaccctc tgacccagcg cctgtaccag tgtctctccc ggaccgtcct
    11641 gccagaccgt ttcccggcac agacgcaggg agtcggcagc cttttcagag ccgctgtagc
    11701 ggggaaacag ctaggcactg tgtgtatcgc cattgttcag ggtgacgttg acgggtttcc
    11761 agatcaaatc gatcagatgc gcaggccgcg gcgatttcag cgaacagatt tgtgaaaacg
    11821 gcagccagat atatgctccg cccgcgatca gttcgagtac cggaccggta cgggagtcgc
    11881 tgtcgctaat ccagtcaaat gcgccgccat tccactgccc ggaagtgtct gttatggcct
    11941 caagcgcgcg gctgcggtga gcatcagctt cctgcgcttc accacgggcg ttacaggcga
    12001 gggcggtcag cagatcctcc atccaggcgg gcggcgggag aataacaccg ggccgctgtt
    12061 cgccctgaaa gcaggcgtgg cggtatatct cacagcggat cagctcgcca aatacctgcg
    12121 cttcgcgagt ataatttgcg tccatccggg cgcatagctg gatctgttgc agtgcccgcg
    12181 accagtcgcc tgccacgcac aataactgaa acaggctgtg gcggcgggag gcctgcgctg
    12241 ggtgcatcct gacctgttgc tctgccatct gaatgccctc caccacggaa taatcccgca
    12301 gcagcgccga caaggtaatc ggtagctcgt tattgtttgt catgatatat ctctccatgt
    12361 tgtgccgggt ttatccggta atggctgtca atgccaatga tgcgatgctc tttccgtgtc
    12421 agatctggca gtacggtgtt atgcgctgtt tccggggcgt attccgggga aagtaaatgc
    12481 aggatatcag ggggagtctt ttcgacaggc cagatagttt catcttcgcc agtggcatcc
    12541 agtacattga gaatagcgtc aatgcttaac ccaccagtga ccatatcctg caatgtctcg
    12601 gcttcggtga ccaggtcacg caatgaatcc agatcctgcg aggagggctc ttcgttaaaa
    12661 ggaaaaggat cagcatggcg attaagatct tgttcgggag ataacaacgc ctcgcggtat
    12721 tcctgataaa gtcgggtcat ggcatttcct ttttccgcac cttctccggt ggaggaggct
    12781 ccatgaacag gaatgatatc gaatggattt tgtgatgtca gatgctgctg cctgaaccaa
    12841 tccagatcca gatattcggt tattgcttcc gacgtgatgg cagtctgcga tatcgttttt
    12901 actgtgctca ggttcggaga tgatggattg ccatgtacaa ggcactatga agataacccc
    12961 cattctatca ggtcgccttc attcagacgc atacggtgat gaggttccac aatctggtcg
    13021 tttaccgtac agcaatattc ggtagactga ttgataatcc accatgct
//
LOCUS       DAJVPP010000105        13022 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVPP010000105 DAJVPP010000000
VERSION     DAJVPP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.105"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1582)
                     /gene="speA"
                     /locus_tag="OQM34_003219"
     CDS             complement(<1..1582)
                     /gene="speA"
                     /locus_tag="OQM34_003219"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1453552.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDIT"
     gene            complement(1591..1722)
                     /gene="yqgB"
                     /locus_tag="OQM34_003220"
     CDS             complement(1591..1722)
                     /gene="yqgB"
                     /locus_tag="OQM34_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1453553.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            1858..2073
                     /locus_tag="OQM34_003221"
     CDS             1858..2073
                     /locus_tag="OQM34_003221"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453554.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2377..3531
                     /gene="metK"
                     /locus_tag="OQM34_003222"
     CDS             2377..3531
                     /gene="metK"
                     /locus_tag="OQM34_003222"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1453555.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            3968..5362
                     /gene="galP"
                     /locus_tag="OQM34_003223"
     CDS             3968..5362
                     /gene="galP"
                     /locus_tag="OQM34_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1453556.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5439..5936
                     /locus_tag="OQM34_003224"
     CDS             5439..5936
                     /locus_tag="OQM34_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1453557.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6031..6738
                     /gene="endA"
                     /locus_tag="OQM34_003225"
     CDS             6031..6738
                     /gene="endA"
                     /locus_tag="OQM34_003225"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1453558.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            6818..7549
                     /gene="rsmE"
                     /locus_tag="OQM34_003226"
     CDS             6818..7549
                     /gene="rsmE"
                     /locus_tag="OQM34_003226"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1453559.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7562..8512
                     /gene="gshB"
                     /locus_tag="OQM34_003227"
     CDS             7562..8512
                     /gene="gshB"
                     /locus_tag="OQM34_003227"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1453560.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            8621..9184
                     /locus_tag="OQM34_003228"
     CDS             8621..9184
                     /locus_tag="OQM34_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1453561.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9184..9600
                     /gene="ruvX"
                     /locus_tag="OQM34_003229"
     CDS             9184..9600
                     /gene="ruvX"
                     /locus_tag="OQM34_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1453562.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(9776..10756)
                     /locus_tag="OQM34_003230"
     CDS             complement(9776..10756)
                     /locus_tag="OQM34_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1453563.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            10774..11478
                     /gene="yggS"
                     /locus_tag="OQM34_003231"
     CDS             10774..11478
                     /gene="yggS"
                     /locus_tag="OQM34_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1453564.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRLAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11496..12062
                     /gene="yggT"
                     /locus_tag="OQM34_003232"
     CDS             11496..12062
                     /gene="yggT"
                     /locus_tag="OQM34_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1453565.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12059..12349
                     /locus_tag="OQM34_003233"
     CDS             12059..12349
                     /locus_tag="OQM34_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1453566.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12357..12950
                     /locus_tag="OQM34_003234"
     CDS             12357..12950
                     /locus_tag="OQM34_003234"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1453567.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
BASE COUNT         3132 a         3476 c         3346 g         3068 t
ORIGIN      
        1 aggtaatatc cagcagcaca gcgcgacgtt ccggcacttg atccagccct tccagcggta
       61 gaaccgggaa caactggtcg atcccccatg cgtccggcat tgactggaac agcgagaaat
      121 tgacgtacat tttgtccgcc atacgttcct gcagttcgtc gattatcgga cgatgcgcac
      181 ggttttgcgg atccagttgc ttctgcactt catggcacat gctcagatag agttgttctg
      241 cccatgcacg ttcttgcagg ctaaaggtgc cggaagagta gccgatatga atgtcgtgca
      301 gatccatctg gctgtcgtgt aaccattcac gcaaagaacg gcgagttccc ggttcgtgca
      361 tttcctgcca ggtttcccac atgctttgca gcgcacgcgg cgcatcttct acgggcgcgg
      421 tcggcaccgt atattcgtta cgttccacgc cgatgatatt cgacaccagc acggtatggt
      481 gcgcagtcac cgcacggccc gattcggtga ttaccgtcgg atgcggcaga ccattttctt
      541 cacacgcatc gccaatcgcc cagatgatgt tgttggcgta ttcattgagg ccatagttta
      601 cggagcagtc agactgcgaa cgggtgcctt cataatccac gcccagaccg ccgccgacgt
      661 cgaagcactg aatattgacg cccagcttgt gcagttccac atagaaacgc gcggattcac
      721 gaacgcctgt cgcgatatcg cgaatattcg ccatctgcga accgaggtgg aagtgcagta
      781 gttgcaggct gtcgagacgc ccggcttcac gcagggtttc aaccagttgc agtacctgag
      841 tcgcagccag gccgaacttc gatttttccc cgccggagga ctgccattta cccgagccct
      901 gcgaagccag acgtgcacgc acgcccagac gaggaacgac attcagacgt tctgcttcat
      961 ccagcacaat ggcgatttct gacatcttct caatgaccag atagaccttg tgccccatct
     1021 tctcgccaat taatgccagg cggatatatt cgcggtcttt ataaccgttg cagacgatga
     1081 cgctacgagt catgccagca tgtgccagca ctgccatcaa ctcggctttg gaaccggctt
     1141 ccagacccag cggttcaccc gaatgaatca gggactcgat cacgcggcgg tgctggttaa
     1201 ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc gtaggattcc ctcgcacgtt
     1261 tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat ctgtgggaaa cagaacagtg
     1321 caggcagacg ctgaccctgt gcttcacgag ttttcactaa ctgcgcgaga tcgacgcgag
     1381 cttccgggac gtccgggtcc gggcacacgc tgatgtggcc cagctcgtta acgtcatagt
     1441 agttattgcc ccaccaggca atattgtaag tacgcagcat cttgctggct tcctgggagc
     1501 tcattgcaac ctcctgcatg gagcgtagta caccgtgttc gcccgctgac gaaggcaaac
     1561 ccatagacat gtcgtcagac atagcgaacc tcaaattatt ttattaagtg taaaacagtt
     1621 aacgactgtc gcagcctgga actgcgataa caacccataa gcacatggat tttccagcag
     1681 tgtatgctga cgctccgact gcgcaaccgg tttctttttc ataacattat taagcacata
     1741 accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa actcttgttt ttacaagagc
     1801 gcccttgttc agtcctcagt aactgtaacc agctcttgaa tcctgagaag cgccgagatg
     1861 ggtataacat cggcaggtat gcaaagcaga gatgcagagt gcggggaacg agtcttcacc
     1921 agaacggtga gacaggttaa gcagcagaca acggttcatt atttcgtatc acctccacgg
     1981 ccgcctgtta agacgaaccc acaagccaaa actctgattt caacccggct ggaagtggca
     2041 acacgaaaga aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt ataccccaca
     2101 atggcagaaa attgcaaaag ataaatacgc agaatgccgg cattgtcagg aaaaatttcc
     2161 agccacgttt ttaacagaat gagacacgat tcaaaaaaaa gtggaaatcg ggtgaagaat
     2221 tgacctaaaa tagccatcca gatgttaatc catccatacc gattaacact cagactgcca
     2281 gtgtttttaa cctgcagagt cgtggtagga tccgctacca cagaaaatcc acacaacagt
     2341 ttgagctaac caaattctct ttaggtgata ttaaatatgg caaaacacct ttttacgtcc
     2401 gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatctctga tgccgtttta
     2461 gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta cgtaaaaacc
     2521 ggcatggttt tagttggcgg cgaaatcacc accagcgcct gggtagacat cgaagagatc
     2581 acccgtaaca ccgttcgcga aattggctat gtgcattccg acatgggctt tgacgctaac
     2641 tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg acatcaacca gggcgttgac
     2701 cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg ctacgcaact
     2761 aatgaaaccg acgtgctgat gccagcacct atcacctatg cacaccgtct ggtacagcgt
     2821 caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc gaaaagccag
     2881 gtgactttcc agtatgacga cggcaaaatc gttggtattg atgctgtcgt gctttccact
     2941 cagcactctg aagagatcga ccagaaatcg ctgcaagaag cggtaatgga agagatcatc
     3001 aagccaattc tgcccgctga atggctgact tctgccacca aattcttcat caacccgacc
     3061 ggtcgtttcg ttatcggtgg cccaatgggt gactgcggtc tgactggccg taaaattatc
     3121 gttgatacct acggcggcat ggcgcgtcac ggtggcggtg cattctctgg taaagatcca
     3181 tcaaaagtgg accgttccgc agcctacgca gcacgttatg tcgcgaaaaa catcgttgct
     3241 gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt ggctgaaccg
     3301 acctccatca tggtagaaac tttcggtact gagaaagtgc cttctgaaca actgactctg
     3361 ctggtacgtg agttcttcga cctgcgccca tacggactga ttcagatgct ggatctgctg
     3421 cacccgatct acaaagaaac cgcagcatac ggtcactttg gtcgtgaaca tttcccgtgg
     3481 gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta atctttcttc
     3541 acctgcgttc aaaggccagc ctcgcgctgg cctttttctt ttacctcacc gcggtggata
     3601 ggcgttcacg ccgcatccgg caaaaaaact gcccgcacaa taacatcatt cttcctgatc
     3661 acgtttcacg gcaaattacc atcacaactg aaaccgatta caccaaccac accagcaaaa
     3721 tatttgtaat attttcatat tattattcag ctttcacagt tgttacattt cttttcagta
     3781 aagtcttaat tgcagatgac agcgtttaat ctatgatgat ataactcaat tattttcatg
     3841 cccttaaatc ataactaaga taaatgttag tgtaagcgat tacactgatg tgatttgctt
     3901 cacatctttt tacgtcgtac tcacctatct taattcacaa taaaaaataa ctatattgga
     3961 gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac aaggcaatga cgtttttcgt
     4021 ctgcttcctt gccgctctgg cgggattact ctttggcctg gatatcggtg taattgctgg
     4081 cgcactgccg tttattgcag atgaattcca gataacttcg cacacgcaag aatgggtcgt
     4141 aagctccatg atgttcggtg cggcagtcgg tgcggtgggc atcggctggc tctcctttaa
     4201 actcgggcgc aaaaagagcc tgatgatcgg cgcaatcctg tttgttgccg gttcactgtt
     4261 ctctgcggct gcgccaaacg ttgaagtact gattctttcg cgcgttctgc tggggctggc
     4321 ggtgggtgtg gcctcttata ccgcaccact gtacctgtca gaaattgcgc cggaaaaaat
     4381 tcgcggcagt atgatctcga tgtatcagtt gatgatcact atcgggatcc tcggtgctta
     4441 tctttctgat accgccttca gctacaccgg tgcatggcgc tggatgctgg gtgtgattat
     4501 catcccggca attttgctgc tgattggtgt cttcttcctg ccagacagcc cacgttggtt
     4561 tgccgccaaa cgccgttttg ttgatgccga acgcgtgctg ctacgcctgc gtgacaccag
     4621 cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt ttgcaggtta aacagagtgg
     4681 ctgggcgctg tttaaagaga atagcaactt ccgccgcgcg gtgttccttg gcgtactgtt
     4741 acaggtaatg cagcaattca ccgggatgaa cgtcatcatg tattacgcgc cgaaaatctt
     4801 cgaactggcg ggttatacca acaccaccga gcaaatgtgg gggacagtga ttgtcggcct
     4861 gaccaacgta cttgccacct ttatcgcaat cggccttgtt gaccgctggg gacgtaaacc
     4921 aacgctaacg ctgggcttcc tggtgatggc tgctggcatg ggcgtactcg gtacaatgat
     4981 gcatatcggt atccactctc cgtcggcgca gtatttcgcc atcgccatgc tgctgatgtt
     5041 tattgtcggt tttgccatga gtgccggtcc gctgatttgg gtactgtgct ccgaaattca
     5101 gccgctgaaa ggccgcgatt ttggcatcac ctgctccacc gccaccaact ggattgccaa
     5161 catgatcgtt ggcgcaacgt tcctgaccat gctcaacacg ctgggtaacg ccaacacctt
     5221 ctgggtgtat gcggctctga acgtactgtt tatcctgctg acattgtggc tggtaccgga
     5281 aaccaaacac gtttcgctgg aacatattga acgtaatctg atgaaaggtc gtaaactgcg
     5341 cgaaatcggc gctcacgatt aatctcctca ggcttcctcc cgtcgcggag gaagccgcct
     5401 cttgcagtca tcttttcttc gctctatcct ctgccgctat gaaaacatcc cgtctcccta
     5461 tcgccatcca acaggccgtt atgcgccgcc tgcgggaaaa actcgcccag gccaacctga
     5521 agctagggcg taactacccg gagccaaaac tctcttacac ccagcgcgga acctccgccg
     5581 gaacagcctg gctggaaagc tatgaaatcc gcctcaatcc cgttttgctg ttggaaaaca
     5641 gtgaagcttt tattgaagaa gtggtaccgc acgaactggc acatttgctg gtatggaaac
     5701 atttcggccg cgtagcgcca catggcaaag agtggaagtg gatgatggaa agcgtgctgg
     5761 gcgttcccgc ccgtcgtacg catcagttcg aactgcaatc cgtgcgtcgc aacaccttcc
     5821 cctaccgctg taagtgccag gaacatcagc ttaccgtacg ccgccataat cgcgtagttc
     5881 gtggcgaggc catctatcgc tgtgttcact gcggtgaaca gctggttgcg aaataaccac
     5941 ctgaactatc aggaactttc ctgatctggc tgattgcata ccaaaacaac tttcgctacg
     6001 ttgctggctc gttttaacac ggagtaagtg atgtaccgtt atttgtctat tgctgcggtg
     6061 gtactgagcg cagcattttc cggcccgacg ttggccgaag gtatcaatag tttttctcag
     6121 gcgaaagccg cggcggtaaa agtccacgct gatgcgcccg gtacgtttta ttgcggatgt
     6181 aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc aatcgtgcgg ctatcaggtg
     6241 cgcaaaaatg aaaaccgcgc cagccgcgta gagtgggaac acgtcgttcc cgcctggcaa
     6301 ttcggtcacc agcgccagtg ctggcaggac ggtggacgta aaaactgcgc taaagatccg
     6361 gtctatcgca agatggaaag cgatatgcat aacctgcagc cgtcagtcgg cgaggtgaat
     6421 ggcgatcgcg gcaattttat gtacagccag tggaatggcg gcgaaggcca gtacggtcaa
     6481 tgcgccatga aggtcgattt caaagaaaaa gctgccgaac caccagcgcg tgcacgcggt
     6541 gccattgcgc gcacctactt ctatatgcgc gaccaataca acctgacact ctctcgccag
     6601 caaacgcagc tgttcaacgc atggaacaag atgtatccgg ttaccgactg ggagtgcgag
     6661 cgcgatgaac gcatcgcgaa ggtgcagggc aatcataacc cgtatgtgca acgcgcttgc
     6721 caggcgcgaa agagctaacc tacactagcg ggattctttt tgttaacccc taccccacgc
     6781 gtacaaccgc gtggggagac gacgcggatt tttaactatg cgtatccccc gcatttatca
     6841 tcctgaacca ctgaccagcc attctcacat cgcgctttgc gaagatgccg ccaaccatat
     6901 cgggcgcgta ctgcgcatgg ggccggggca ggcgttgcaa ttgtttgacg gtagcaacca
     6961 ggtctttgac gccgaaatta ccagcgccag caaaaaaagc gtggaagtga aggtgctgga
     7021 aggccagatc gacgatcgcg aatctccgct gcatattcac ctcggtcagg tgatgtcgcg
     7081 tggtgaaaaa atggaattta ctatccagaa atcgatcgaa ctcggtgtaa gcctcattac
     7141 gccacttttt tctgagcgct gcggcgttaa actggatagt gaacgtctga acaagaagct
     7201 tcagcagtgg cagaagattg caattgctgc ctgtgagcag tgtggtcgta accgggtgcc
     7261 ggaaatccgt ccagcgatgg atctggaagc ctggtgtgca gagcaggatg aaggactgaa
     7321 actgaatctt cacccgcgcg ccagtaacag catcaatacg ttgcctttac cggttgaacg
     7381 cgtccgcctg ctgattggcc cggaaggcgg tttatcggca gatgaaattg ccatgactgc
     7441 ccgctatcaa tttactgata tcctgttggg acctcgcgtt ttgcgtacag agacaactgc
     7501 gctcaccgcc attaccgcgc tacaagtacg atttggcgat ttgggctaac ggagaagaat
     7561 aatgatcaag ctcggcatcg tgatggaccc catcgcaaac atcaacatca agaaagattc
     7621 cagtttcgct atgttgctgg aagcacagcg tcgtggttac gaacttcact atatggagat
     7681 gggcgatctg tatctgatca atggcgaagc ccgcgcccat acccgcacgc tgaacgtgaa
     7741 gcagaactac gaagagtggt tttcgttcgt cggtgaacag gatctgccgc tggccgatct
     7801 cgatgtgatc ctgatgcgta aagacccgcc gtttgatacc gagtttatct acgcgaccta
     7861 tattctggaa cgtgccgaag agaaagggac gctgatcgtt aacaagccgc agagcctgcg
     7921 cgactgtaac gagaaactgt ttaccgcctg gttctctgac ttaacgccag aaacgctggt
     7981 tacgcgcaat aaagcgcagc taaaagcgtt ctgggagaaa cacagcgaca tcattcttaa
     8041 gccgctggac ggtatgggcg gcgcgtcgat tttccgcgtg aaagaaggcg atccaaacct
     8101 cggcgtgatt gccgaaaccc tgactgagca tggcacttgc tactgcatgg cgcaaaatta
     8161 cctgccagcc attaaagatg gcgacaaacg cgtgctggtg gtggatggcg agccggtacc
     8221 gtactgcctg gcgcgtattc cgcagggggg cgaaacccgt ggcaatctgg ctgccggtgg
     8281 tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa atcgcccgtc agatcgggcc
     8341 gacgctgaaa gaaaaagggc tgatttttgt tggtctggat atcatcggcg accgtctgac
     8401 tgaaattaac gtcaccagcc caacctgtat tcgtgagatt gaagcagagt ttccggtgtc
     8461 gatcaccgga atgttaatgg atgccatcga agcacgttta cagcagcagt aacccacctt
     8521 agcgagaagg atctcgttga gactctgagt gacagcgccc ttctttccac gcatactggg
     8581 cgctgttgct tttttgaacc aggaaacaga acctctgaca atgaatttac agcatcactt
     8641 tcttattgcc atgcctgctc tccaggatcc gattttccgt cgttccgtgg tctacatttg
     8701 cgaacataat accaatggtg caatggggat catcgtcaac aagccgctgg aaaatctcaa
     8761 aattgaaggg attctggaaa agctgaagat cacgccggag ccgcgtgatg aatcaatccg
     8821 tctggataaa ccggttatgc tcggcggccc actggctgaa gatcgcgggt ttattttgca
     8881 tactccgccc tccaattttg cttccagcat tcgcatttca gacaacacgg tgatgaccac
     8941 ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa ccgtctgacg tattggtggc
     9001 tctgggctat gcctcctggg aaaaaggtca actggaacaa gaaattctcg ataacgcgtg
     9061 gctaacggcc ccggcggatc tgaatattct gttcaaaacg ccgattgccg accgctggcg
     9121 cgaggcggca aaactgattg gtgtggatat tctcaccatg cctggtgtgg caggacacgc
     9181 ctgatgagtg gaaccttact cgccttcgac ttcggcacca aaagcattgg cgtagcggtc
     9241 ggccaacgca ttaccggcac cgctcgccct ttgcctgcaa ttaaagcgca ggacggtacg
     9301 ccggactgga acctcatcga acgtttactg aaagagtggc agcctgacga aatcatcgtc
     9361 ggtttgccgc tgaatatgga cggcaccgaa cagccgttaa ccgccagagc gcgcaaattt
     9421 gccaaccgta ttcatggtcg tttcggtgtt gaagtaaagc tccatgacga gcgtcttagc
     9481 actgtggaag cccgttccgg tctttttgaa cagggcggct atcgggcgct caacaaaggc
     9541 aaagttgact ctgcctctgc ggttattatt ctcgaaagct atttcgagca gggatattaa
     9601 ggcgatctaa aagcctggcg gatagtcgtt accgccatct tgccggatgc ggcgtgaacg
     9661 ccttatccag cacacatctg gcagcggctg taggtctgat aagacgcgtt agcgtcgcat
     9721 cagacattta ttgcctttat aggccctata aacttgcgca tcgggcatgg caacgtcaca
     9781 aacgcccttc ccccacccgc tgctgataac tctgctgaaa cgttaacatc cccacctgct
     9841 gcccggtttg aataacatgc ggtaactggt gggttttccc ttcgcgaatc aaattcccca
     9901 ccgcgggagt gttaatcagt aattcaaaca gcgccacgcg tccttcctgt ttatccactt
     9961 ccagcttttg tgacagcact gcccgtaaac tacccgcaag ttgattacgt acgggatctt
    10021 tttcctgcgc cggaaatgaa tccaccagtc gctcaactgc ctgcgcggca ccacgtgtat
    10081 gtaaggttgc cagcaccaga tgcccggttt ctgccgccgt cagcgccaga cggattgtct
    10141 cactgtcacg tagctctccg agcaaaatca catcaggatc ttcccgcaat gcagcccgca
    10201 accccgatgc gaacgtcata cagtgcaaac caatttcccg ctgctggatc aaacatcgct
    10261 ggctggcata gagatattcc acaggatctt ccagcgtcag aatatgcgca tcggcatgtt
    10321 gattaagata gccaaccatc gccgccagcg tggtagattt gccactcccc gtagcccccg
    10381 tcaccagaat caggccattc tcgctcttga gtaattccgg caataccggt ggtgcaccaa
    10441 gctgttcgag ctgtggacag tgcgaaggta acaaccgtaa tgccagagaa ataccttgcc
    10501 gttgcgcgaa cgcactgcca cgcaaccgct ggttttccgc cagcgacacg gcaaaatcca
    10561 gctggccatt ttccagcaat attgcccgct gatcgtcatc cagccactcc cgcagtagct
    10621 cttcgacgtc cggcgcatca aacggcgcag cttccattct gccacgaatg cgccatcgtg
    10681 cgggccaggc gctgcacagg tgtagatccg agacgttatg ctttacacta agggccacaa
    10741 tttcttccat attcatacta agatcctcgg aaaatgaacg atattgcgca taacctggca
    10801 caggtccggg acaaaatctc agcggctgca acgcgttgcg gccgttctcc agaagaaatt
    10861 acgctgcttg cagtcagtaa aacaaaacct gcgagcgcca tcgcagaagc cattgatgcc
    10921 gggcagcgtc aatttggtga aaactacgtt caggaagggg tagataaaat tcgccacttt
    10981 caggaactgg gcgtaacagg attagaatgg cattttattg gcccgttgca gtctaataaa
    11041 agccgcctag tggcagagca tttcgactgg tgtcatacca tcgaccgttt gcgcattgct
    11101 acccgcctca acgagcagcg cctggcagaa cttccccctc ttaacgtact gattcaaatt
    11161 aatattagtg atgaaaacag taagtccggg attcaactgg cagaactgga cgagctggca
    11221 gcggcggtcg ctgaactacc gcgtttacgt ctgcgcgggc tgatggcaat ccctgcgcct
    11281 gagtcagaat atgtaaggca gtttgaagtt gcacgccaaa tggctgtagc atttgccgga
    11341 ctgaaaacgc gctacccgca tatcgacacg ctctctctgg gaatgtcgga cgatatggaa
    11401 gccgccattg cggcaggtag cacgatggtt cgtatcggca ctgcaatttt tggtgcgcgt
    11461 gattactcta aaaaataagg aattaaagga acgccatgaa tacgttgact ttcctgcttt
    11521 caacggtcat tgagctgtat acaatggtgc tgttattacg catctggatg cagtgggctc
    11581 attgtgattt ttacaacccc ttctcacagt ttgtagtgaa ggtagcgcag ccaattatcg
    11641 ggccactgcg ccgcgttatt ccggcaatgg gtcccattga cagcgcctcg ctgctggttg
    11701 cctatattct cagttttatc aaagccattg tgctgtttga agtggtgacc ttcctgccaa
    11761 tcatctggat tgccggttta ctgattctgc tgaaaaccat cggcttgctg attttctggg
    11821 tcctgctggt gatggcgatt atgagctggg taagccaggg tcgtagcccg attgaatacg
    11881 tgctgattca gctggccgat ccgctgctgc gcccgattcg ccgcttgcta ccggcaatgg
    11941 gtgggattga tttctcgccg atgatcctcg ttctgctgct gtatgccatc aatatgggtg
    12001 tcgcagaagt attacaggca accggaaaca tgctgctgcc ggggctgtgg atggcgttat
    12061 gagtgccgta acagttaatg atgacggtct ggttttacgg ctctatattc agccgaaagc
    12121 cagccgtgat tctattgtcg gtttacatgg cgacgaagtt aaagtcgcca ttaccgcgcc
    12181 gccggttgac ggccaggcca acagtcatct ggtgaagttt ctcggtaagc aattccgggt
    12241 tgccaaaagc caggtggtga ttgaaaaagg cgaacttggc cgccacaaac aaattaaaat
    12301 cattaatccg caacaaatcc cgccagaaat cgcggcgtta attaattagg tatcctatgc
    12361 aaaaagttgt cctcgcaacc ggcaatgccg gtaaagtacg tgagctggcg tcgctgctta
    12421 gcgactttgg tcttgatatc gtggcccaaa cagacctcgg cgttgattcc gctgaagaaa
    12481 ccggcctaac ctttatcgaa aacgcgattc tgaaagcgcg ccatgcggca aaagtgaccg
    12541 gtttaccggc aattgccgac gactctggtc tggcggtaga tgcgctgggt ggcgcgccgg
    12601 gtatttactc cgcgcgttat tccggtgaag acgcgaccga tcaaaagaat ctgcaaaaac
    12661 tgttggaaac actgaaagac gtaccggacg accaacgtca ggcgcgtttc cactgcgtgc
    12721 tggtatatct gcgtcacgcg gaagatccga ctccgctggt atgccacggc agctggccgg
    12781 gcgtgattac tcgcgaacca gcgggcactg gtggctttgg ttatgatcca atcttcttcg
    12841 taccttccga agggaaaacc gccgccgaac tgacccgcga agaaaagagc gccatttccc
    12901 accgtggtca ggcgttgaaa ctgctgctgg acgctttacg taatggttaa attaccgccg
    12961 ctgagtctct acattcacat cccgtggtgc gtgcagaaat gcccgtactg cgatttcaac
    13021 tc
//
LOCUS       DAJVPP010000106        12650 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVPP010000106 DAJVPP010000000
VERSION     DAJVPP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.106"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..238)
                     /locus_tag="OQM34_003235"
     CDS             complement(<1..238)
                     /locus_tag="OQM34_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCS1453568.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMR"
     gene            557..1138
                     /gene="mdaB"
                     /locus_tag="OQM34_003236"
     CDS             557..1138
                     /gene="mdaB"
                     /locus_tag="OQM34_003236"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1453569.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHNVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            1169..1483
                     /locus_tag="OQM34_003237"
     CDS             1169..1483
                     /locus_tag="OQM34_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1453570.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1531..3423)
                     /gene="parE"
                     /locus_tag="OQM34_003238"
     CDS             complement(1531..3423)
                     /gene="parE"
                     /locus_tag="OQM34_003238"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1453571.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKSVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3452..4033)
                     /gene="yqiA"
                     /locus_tag="OQM34_003239"
     CDS             complement(3452..4033)
                     /gene="yqiA"
                     /locus_tag="OQM34_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1453572.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4033..4860)
                     /gene="cpdA"
                     /locus_tag="OQM34_003240"
     CDS             complement(4033..4860)
                     /gene="cpdA"
                     /locus_tag="OQM34_003240"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1453573.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4885..5307)
                     /locus_tag="OQM34_003241"
     CDS             complement(4885..5307)
                     /locus_tag="OQM34_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS1453574.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5308..5937)
                     /gene="nudF"
                     /locus_tag="OQM34_003242"
     CDS             complement(5308..5937)
                     /gene="nudF"
                     /locus_tag="OQM34_003242"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1453575.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6142..7623
                     /gene="tolC"
                     /locus_tag="OQM34_003243"
     CDS             6142..7623
                     /gene="tolC"
                     /locus_tag="OQM34_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1453576.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7771..8442
                     /locus_tag="OQM34_003244"
     CDS             7771..8442
                     /locus_tag="OQM34_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1453577.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8448..9608
                     /locus_tag="OQM34_003245"
     CDS             8448..9608
                     /locus_tag="OQM34_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1453578.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9646..10434)
                     /gene="ygiD"
                     /locus_tag="OQM34_003246"
     CDS             complement(9646..10434)
                     /gene="ygiD"
                     /locus_tag="OQM34_003246"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1453579.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10577..11350
                     /gene="zupT"
                     /locus_tag="OQM34_003247"
     CDS             10577..11350
                     /gene="zupT"
                     /locus_tag="OQM34_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1453580.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11408..11578)
                     /locus_tag="OQM34_003248"
     CDS             complement(11408..11578)
                     /locus_tag="OQM34_003248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453581.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11839..12492)
                     /gene="ribB"
                     /locus_tag="OQM34_003249"
     CDS             complement(11839..12492)
                     /gene="ribB"
                     /locus_tag="OQM34_003249"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1453582.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
BASE COUNT         3208 a         3374 c         3073 g         2995 t
ORIGIN      
        1 cccgcataaa gagttttctt aagataagaa taataagtgg cgtaagaaga aaaaatgctg
       61 caatcattgc ttgcaatata cgatcttcgc ctgaaacgga aggtatactg caaatattgc
      121 ctcccgactc aagcatccag tcatacttat tgaaagcaaa aaaagaatct attaaccaat
      181 aagtaagcat tatatatagc aatgcttcaa atattgtttt aattttctgc tttaacatat
      241 atcagtacgc tcataaaaat ccatttataa ttatcaacat gacacgttga tattttcatc
      301 ttttacttat tcaaagccat attttcaatc cccaaaaaaa ccgaaccaaa cgtgtaaatt
      361 attccggtgc gagtaagaat tatgaggaat ggctatcagt attgtcattt tcagaaaata
      421 tttatcctgc atcggtgagt cagagctaga tcagactttt gctaaattcg caaaagactt
      481 tgcacatttt gctaatttca cagtaccgct ctgtgacgta ctatagtcga caaacgtctc
      541 accttgagat taaaaaatga gcaacatcct gattatcaac ggcgcgaaaa aattcgccca
      601 ctccaatggt caactgaacg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct
      661 cgggcataat gtccgcatcg ttcgcgccga cagcgactac aatgtcaaag cggaagtaca
      721 aaactttctc tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc
      781 gtggacagtg aaaaaatata ttgatgatgt attcaccgaa ggtcacggga ctctgtatgc
      841 cagcgatggt cgtacccgca aagatccgtc gaaaaaatac ggttccggca gcctggtaca
      901 gggcaaaaaa tatatgcttt ctctaacctg gaacgcacca atggaagcct tcaccgaaaa
      961 agatcagttc ttccacggcg ttggcgttga cggtgtgtat ctgccgttcc ataaagcaaa
     1021 ccaattcctc ggtatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc
     1081 tgatgttccc cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact
     1141 agagctcagg ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg
     1201 tcctggtcaa catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt
     1261 actgaaagaa gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag
     1321 tttccagtct atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca
     1381 ccttgaagcg catctgcaaa ccccgcacat gaaggcgtac agcgaagccg taaaaggcga
     1441 cgtgctggag atgaatatcc gtattctgca gccagggatt taatcctgcc ttgtttgccc
     1501 ggccatcctg accgggcaat gttctttcct ttaaacctca atctccgcca tgtcgccttt
     1561 ctcttgcaac cagttgcggc gatcttccga gcgtttcttc gccagcagca tatccatcat
     1621 cgcgtcagta cgctgatcgt cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt
     1681 cggatcaagc gtggtttcac gcaattgcat cgggttcatt tcccccagac ctttaaaacg
     1741 ctggacgttc ggcttgcctt tcttgcgttt taattgctca agtacgccct ctttctcttc
     1801 ttccgtcagc gcgtaataaa cctctttccc gagatcaata cggtagagcg gtggcagtgc
     1861 gacgtaaacg tgaccgtgtt tcaccaacgc gcggaagtgt tttacgaaca aagcgcagag
     1921 cagcgtggca atgtgcagac catcagagtc cgcatccgcg aggatacaga ttttgccata
     1981 acgaagctgg ctcagatcgt cgctgtcagg atcgataccg atcgctaccg aaatatcatg
     2041 cacttcctgc gaggccagca cttcgtcgga agaaacttcc caggtgttaa ggatcttacc
     2101 tttcagcggc atgatcgcct gatattcacg atcgcgcgcc tgcttggcag atccgcctgc
     2161 ggagtcacct tccacgagga acagttcggt acggttaagg ccctgcgcgg tacaatcagc
     2221 cagtttgcca ggcagcgccg ggccgctggt cagcttttta cgcaccactt ttttagccgc
     2281 acgcatacga cgctgggcgc tggaaatcgc catctctgcc agcaactccg ccgcctgaac
     2341 gttctggttc agccacagga tgaaggcatc tttcaccacg cccgaaacga atgccgcaca
     2401 ctgacgcgaa gagagacgct ctttggtctg cccggcaaac tgcggatcct gcattttaac
     2461 cgacagaaca taggcgcagc gatcccagat atcttccgcc gacagcttca cgccgcgcgg
     2521 cagaatgttg cggtattcac agaactcacg catcgcgtcc aacaggccct gacgcagacc
     2581 attaacatgg gtaccgccct gcatcgttgg gataaggttg acgtagcttt cggtcagcag
     2641 ttcaccgcct tccggcagcc acagtagcgc ccagtccaca gcttcagtat cgccagcgaa
     2701 attaccgata aacggttttt ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta
     2761 atcattcaga ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct catctttaaa
     2821 ggtaatctca acgccggggc acagtactga tttggccttc agcacatgcg tcaggcgtga
     2881 aacagaaaaa cgcgggctgt caaagaaggt ttcatccggc cagaagtgca cgctggtacc
     2941 ggtattgcgt ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt cgccattttc
     3001 aaaggcgatg ttataaacct gaccatcgcg gcgcacgtta acttctacgc gcttcgacaa
     3061 ggcgttaact accgaaatcc ccacgccatg caggccgcca gagaactggt aatttttgtt
     3121 agagaattta ccgcctgcat gcagacggca aagaatcagt tcaaccgccg gtactccttc
     3181 ttccgggtga atatccaccg gcatcccgcg cccatcgtca atcacttcta acgactggtc
     3241 agcatgtaag attacatcca cgcgttttgc gtgacccgcc agtgcttcat cgacactgtt
     3301 atcaataact tcttgcccca aatggttagg gcgagtggta tcggtataca tccccggacg
     3361 gcggcgaacc ggctcaagcc cggtgagtac ctcaatggca tcagcgttat aagtttgcgt
     3421 catggtttaa gttagcaatt cgagttgatc gtcagagatg gtgcagacca agaaaatcga
     3481 cgatcgggtt gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag
     3541 tctggcggca ggaagcgtag tacgccaccg cctggcggta atccagcact tcatctcccg
     3601 tctgttgcag cagccagatc aaatccggcg cttccagcgg gtcaatctgc atgactttga
     3661 gatcgtaaat atggcgtgac tctagcacat attgctgccc ggtgtagggg ttctcgttct
     3721 gaccgagata gtccgtcagc aattcaaacg ggcgtaccgc cgggtttacc accactgcgg
     3781 gcagcataaa acattgcgac aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa
     3841 tacccagcga atcgccgcca tgttccagga caatggattc cagcagttct gccgcgtcgg
     3901 aaggatacgg cggcaactgc ggaatgatca tctcaacgtc agggtgatgt tccgccagcc
     3961 agttttttaa caagctcgct tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa
     4021 gaagcgtaga catcagtagc cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag
     4081 gcgatgcacc tcggtggtca gcgtgccatc agcatgtaac tcgagagtac gccagccggg
     4141 cgcgatagta tccagcgtaa agttggaaca gtgcggctta aactgcacac aggtcgacgg
     4201 cgttgccagc aggcggcgac cgttccagtc gagatccagc tcctgatgaa tatgaccgca
     4261 cagcaagtat ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc ccgcgttacg
     4321 cagactgtgt tgatcgagcc aactacaacc cgcaggtagc ggatgatgat gcagcagcag
     4381 caacgtatgg cgttctggcg catcggccag tttacgttcc agccactcaa gctgaaactc
     4441 gctcagctca ccgtgcggca cgccaaacac ctggctatcc agcaacagga tttgccattg
     4501 ctcaccaata aacacccgct tcgccgggga gatacccgca tcctgtaacg cgctgtacat
     4561 cgcgggctgg aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat
     4621 gccttcagcg aaatgctgat aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc
     4681 gacaatcagg tcgaattcgt gctggtgtgg acgaatcgcc tccagcaccg cctggtagct
     4741 ttcccaggtg tttaccccta acagggcttc gtgcttttgt gcaaacaggt gagtatcggt
     4801 aatttgtaaa atccttcctc tggcctcacc agccagagga agggttaaca ggctttccaa
     4861 atggtgtcct taggtttcac gacgctagta aaccggaatc gccatcgctc catgtgctaa
     4921 acagtatcgc aaccagtccg ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg
     4981 caacttttta ttaggataat cataccgcgc tttgaagcga aaaatctgct ggcttgaaca
     5041 cacttcagcc accatcgcgt catgatacag acgcaccgtc attgacggaa ggctccagta
     5101 actgatcgcg ggcgcagtct gttctattgt caccagggta gtgtatcggg tcgattccac
     5161 aatcgtcagc cgatattgtg cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc
     5221 attgcgcggt aacaaacggc gcaattgtga aaagttcatc tcgcacaggc gcatcatttc
     5281 aggaaagtca ggtgtgtaac gcttcattta tgcccactca ttttttaacg cttgatgatg
     5341 cagctgcagc cattgcaaag cgatgaccga cgctgcgttg tcgattttcc cctcttctac
     5401 ccactggtat gcctgttccc ggcttaccac atgaacgcga atatcttcgt tttcatcagc
     5461 cagaccatga ataccgcttg cggtcgtggc gtccacttcg cccaccataa ttgacgaacg
     5521 ctcactggtg ccccccgggc ttgccaggaa acttaacacc ggtttggtcc gtttgactat
     5581 cagtcccgcc tcttcaatcg cttcgcgacg ggcaacatct tccacacttt caccctcttc
     5641 aatcatcccg gcaaccatct ccagtagcca gggggtttcg ctggtgtcgt acgcggcaat
     5701 ccgaatctgc tcaatcagca caacttcatc acgcactggg tcaaagggta gcaagactgc
     5761 ggcgtgaccg cgctcaaaaa tttcccgccg tacctcatga ctcatttgcc cgttgaatag
     5821 acgatgacga aatctataaa gatctaatga aaaaaagccg cgataaagtg tttctcgtgc
     5881 aataatttct acatcgtttt tgccaaatgt aacgggcagg ttgtctggct taagcattgt
     5941 taatgtccta gcactaatag tgaattaaat gtgaatttca gcgacgtttg actgccgttt
     6001 gagcagtcat atgttaaatt gaggcacatt aacgccctat ggcacgtaac gccaaccttt
     6061 tgcggtagcg gcttctgcta gaatccgcaa taattttaca gtttgatcgc gctaaatact
     6121 gcttcaccac aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt
     6181 tctgggttca gttcgttgag ccaggccgag aacctgatgc aagtttatca gcaagcacgc
     6241 cttagtaacc cggaattgcg taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt
     6301 aatgaagcgc gcagtccatt actgccacag ctaggtttag gtgcagatta cacctatagc
     6361 aacggctacc gcgacgcgaa cggcatcaac tcgaacgcga ccagtgcgtc cctgcagtta
     6421 actcaatcca tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca
     6481 gggattcagg acgtcacata tcagaccgat cagcaaacct tgatcctcaa caccgcgacc
     6541 gcttatttca acgtgttgaa tgctattgac gttctttcct atacacaggc acaaaaagaa
     6601 gcgatctacc gtcaattaga tcaaaccacc caacgtttta acgtgggcct ggtagcgatc
     6661 accgacgtgc agaacgcccg cgcgcagtac gataccgtgc tggcgaacga agtgaccgca
     6721 cgtaataacc ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa ctactatccg
     6781 gaactggcgg cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg
     6841 ctgctgaaag aagccgaaaa acgcaacctg tcgctgttac aggcacgctt gagccaggac
     6901 ctggagcgcg agcaaattcg ccaggcgcag gatggtcact taccgactct ggatttaacg
     6961 gcttctaccg ggatttctga cacctcttat agcggttcga aaacccgtgg tgccgctggt
     7021 acccagtatg acgatagcaa tatgggccag aacaaagttg gcctgagctt ctcgctgccg
     7081 atttatcagg gcggaatggt taactcgcag gtgaaacagg cacagtacaa ctttgttggt
     7141 gccagtgagc aactggaaag cgcacatcgt agcgtcgtgc aaaccgtacg ttcctccttc
     7201 aacaacatta atgcttctat cagtagtatt aacgcctaca aacaagccgt agtttccgcg
     7261 caaagctcat tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac cattgttgat
     7321 gtgttggatg cgaccaccac gctgtacaac gccaagcaag agctggcgaa tgcgcgttat
     7381 aactacctga ttaatcagct gaatattaag tcagccctgg gtacgttgaa cgagcaggat
     7441 ttgctggcac tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc
     7501 ccgcaaacgc cggaacagaa tgctattgct gatggttatg cgcctgatag cccggcaccc
     7561 gtcgttcagc aaacatccgc acgcactacc accagtaacg gtcataaccc tttccgtaac
     7621 tgatgacgac gacggggctt cggccccgtc tgaacgtaag gcaacgtaaa gatacgggtt
     7681 atctgccgca ttcttccccc ttctcgcttc aatttcgacc agccatcctc tattctgatg
     7741 ggtatttacc actggtcccg gaagacaaaa atgaaacgga caaaatccat acgccacgca
     7801 tcgttccgca aaaactggag cgcacgccat ctgacaccag tcgctctcgc ggttgccact
     7861 gtttttatgc tggctggctg tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct
     7921 gacgactgtt cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat
     7981 gcgctgaaag aagccgaacg tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct
     8041 gaatttggtg aaggtcagtg tcagcaagca ccagcccagg ctggcatggc accagaaaac
     8101 caggcgcagg ctcagcaatc cagcgggagt ttctggatgc cgctgatggc cggttacatg
     8161 atggggcgtc tgatgggcgg cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa
     8221 aacccggcca gtccggctta cggtaaatat accgacgcga cgggtaaaaa ctatggcgca
     8281 gcccagccag gccgcaccat gaccgtaccg aagacggcaa tggcaccaaa accggcgacc
     8341 accactaccg ttacccgtgg cggttttggt gaatctgttg ccaaacaaag cactatgcag
     8401 cgtagcgcaa ccggcacctc ttctcgttca atgggtggct gataccgatg gaaagagtca
     8461 gtattaccga gcgcccggac tggcgtgaga aagcccacga atacggtttc aattttcaca
     8521 ccatgtacgg cgagccgtac tggtgtgaag atgcttacta caagttgacc ctcgcccagg
     8581 ttgaaaagct ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa
     8641 aagtgatcgc cagcgatgag ctgatgacca aattccgcat accaaaacac acctggagtt
     8701 ttgtgcgtca atcatggctg actcaccagc catcgcttta ttcgcgtctt gatctggcgt
     8761 gggatggcac tggcgaacct aaacttctgg aaaataacgc cgatacgcca acgtcactat
     8821 acgaggcggc gttctttcag tggatctggc tggaagatca gcttaacgcc ggtaacttgt
     8881 cggagggcag cgaccagttt aacagtctgc aagaaaaact gatcgatcgc ttcgttgagc
     8941 tgcgtgaaca gtatggcttc cagttgctgc atctcacctg ctgtcgcgac acggtggaag
     9001 atcgcggaac cattcagtat ttgcaggact gcgcaaccga agctgaaatc gccaccgagt
     9061 tcctctacat cgacgagata ggtttaggcg aaaaaggcca gttcacggat ttacaggatc
     9121 aggtgatttc taacctgttc aagctttatc cgtgggaatt tatgctgcgc gaaatgttct
     9181 ccaccaagct ggaagatgct ggcgtacgct ggctggaacc ggcgtggaag agcattatct
     9241 ccaacaaggc acttctaccg ctactgtggg agatgttccc gaatcacccg aacctgctgc
     9301 ccgcttattt tgcggaagat gatcatccgc aaatggaaaa atatgtggtt aaaccgatct
     9361 tctcccgtga aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt gaagcagcgg
     9421 aaggtccgta tggcgaagaa gggatgattg ttcagcaatt ccacccgtta ccgaaattcg
     9481 gcgacagcta tatgctgatt ggtagctggc tggtgaacga tcaacctgcc ggaattggca
     9541 ttcgtgaaga ccgtgcgttg atcacccagg atatgtctcg gttttatcca catatttttg
     9601 ttgaataagc cacgataccg gatggcactc gccatccggt aattgttagc ctatctgcac
     9661 cgacagcata cttaaactgc ccatttctat accatcgacc ggaatggtaa ttggctcctg
     9721 cccatcccac acacctaaca catacaacaa cggcagatag tgctctggcg ttgggttcga
     9781 taacgcgcca ccttcatggt cgagataatt caccagagga tgctgctcta ccggcccttg
     9841 ccacgtcaga ttcgctttca catactcatt aaacgacgtc gcccacggat acggtgaact
     9901 atcaccgtgc cacttcactg tgcgcaggtt atgcaccacg ttaccgctgg cgaccaacat
     9961 tattccttca tcacgcagcg ccgcgagttt gcgccccatt tcgaaatgcc aggcggcagg
    10021 tttgctactg tcgatactca actgcaccat agggatatcg gcgtcaggat acatcttaat
    10081 cagcacgccc cacgagccgt ggtcaaagcc ccaggcttct ttatccagcg tcaccgggat
    10141 cggcgctaac agctcaacca gacgctgtgc cagcgcaggc gaacccggag caggataatg
    10201 cgtatcgtac agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat
    10261 cgcggtcact cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg
    10321 gcgtggcaat gtcatcccca acttctgcca gctgcgggta tacaaattat cttccagcac
    10381 gttcatcgga ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat
    10441 atccttaact aaaggtgtca ttttgatatc ctcacaatac gcttgttcgg cggagtaaga
    10501 acccggataa caatgatgat gatcatcagt tattttgacg atctgcctga aggtgaagat
    10561 ttataaggag ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac
    10621 gtttattggc gcgtttctcg gcgttctcgg acaaaaaccc tcgaaccgct tactggcgtt
    10681 ttcgctaggt tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc
    10741 cgcactagca gctgaaggaa tgtcgccggt gttgggttat ggaatgttta tcttcggtct
    10801 gcttggctac tttggcctgg accgcatgtt gccgcatgcg catccgcagg atttaatgca
    10861 aaaatcggtg cagctgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg
    10921 catcagtctg cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct
    10981 ggagctggga tttggcatcg cactggccgt cgcgttgcac aatatccctg aaggtctggc
    11041 agtggcaggc ccggtttatg cggcaacggg gtctaaacgc accgcgattc tgtgggcggg
    11101 gatttctgga ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat
    11161 gatttccccg gtggtcatgg cggcaatcat ggcggcggtt gcaggaatta tggtggcgct
    11221 ctcggttgat gaattaatgc cgctcgccaa agagattgac cctaataata accccagcta
    11281 tggcgtacta tgtggaatgt cagtgatggg attcagttta gtgctgctac aaacggcggg
    11341 aattggttaa aaacaacatc agaagttgtc cagcacatgg acaacttctg ccagcttcac
    11401 gaaagcctta ttctaccttt tcggctgtct cttctctcgt actgtttaag tatttgttcc
    11461 gcgcttcaag cagtgcgtct ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca
    11521 ggaaataacc caccacaacc agagcaatca atacaatcca gtaccaggcg ataaacatcc
    11581 ttttaccctt aatatttaac aatactaaac atttagcgca taaatttcac atatcctttt
    11641 tcggatatat tcatatggtc gcataataac ggaacaatta tatgattttg atcataatga
    11701 aaaagaatgc ctgatattaa tgaaattttc tcatagaaag agaagcggaa ttaacatctt
    11761 gcatagcagg gcaaaataaa aaccgaagcc agctacaact tcggtttctt gattaaggca
    11821 gtaattaagc agcggttttc agctggcttt acgctcatgt gcctgacggt atgccaccag
    11881 gtcttcaata gtcacgagcg ccatattgtg tttattggca aactcaatgc actctggtgc
    11941 acgcgccatc gtgccatcgt cattagtcag ctcacacagc acaccagccg gtttaaagcc
    12001 tgccagcgtc atcaaatcaa tagtcgcttc agtatgaccg ccacgcgtca gtacaccacc
    12061 tgcctgcgcg cgaagtggga aaacgtggcc cgggcgattc agatcagaag gttttgcgcc
    12121 atcggcaatc gctgcgcgaa cggtcgtaat acgatcagcg gcagaaacac cggtagtcac
    12181 accttcagcc gcttcaatgg tcacggtaaa accggtgcca taggcgctgg tgttattttc
    12241 taccatcatt ggcagatcaa gttgtttacg gcgatcttca gtgatgcaca ggcaaacaat
    12301 accgctaccg tggcgaatgg tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa
    12361 gatcatatcg ccttcgtttt cacggtcttc atcatcaagc accattacac cgcgtccttc
    12421 acgcagcgca gccagtgcat tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag
    12481 cgtctgattc atggtaaaaa aacctcacta aaattatggt taccagaatc agggcagtct
    12541 taggagtggc ggcatatagc caaaataacg tgagcgggtc catgcccgac agaatcgtta
    12601 ctctctccca tccggactct aaccgtcggc cccggaatta caccggatct
//
LOCUS       DAJVPP010000107        12635 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVPP010000107 DAJVPP010000000
VERSION     DAJVPP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.107"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1038
                     /locus_tag="OQM34_003250"
     CDS             <1..1038
                     /locus_tag="OQM34_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1453583.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="OQM34_003251"
                     /pseudo
     CDS             1039..3204
                     /locus_tag="OQM34_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
     gene            3218..4129
                     /locus_tag="OQM34_003252"
     CDS             3218..4129
                     /locus_tag="OQM34_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCS1453584.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="OQM34_003253"
     CDS             4129..5424
                     /locus_tag="OQM34_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCS1453585.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSISGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="OQM34_003254"
     CDS             5469..5699
                     /locus_tag="OQM34_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453586.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="OQM34_003255"
     CDS             5677..6177
                     /locus_tag="OQM34_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCS1453587.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLIATARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="OQM34_003256"
     CDS             6177..7595
                     /locus_tag="OQM34_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCS1453588.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIVSASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="OQM34_003257"
     CDS             7595..8443
                     /locus_tag="OQM34_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453589.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="OQM34_003258"
     CDS             8443..8898
                     /locus_tag="OQM34_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCS1453590.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSGTREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="OQM34_003259"
     CDS             8901..9593
                     /locus_tag="OQM34_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCS1453591.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="OQM34_003260"
                     /pseudo
     CDS             9603..10934
                     /locus_tag="OQM34_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
     gene            10935..>12635
                     /locus_tag="OQM34_003261"
     CDS             10935..>12635
                     /locus_tag="OQM34_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS1453592.1"
                     /translation="MVNYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPRTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQAPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     D"
BASE COUNT         3397 a         3122 c         3333 g         2783 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc gggggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatgag acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tcctgaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 tcaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tatagccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg tcagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaagcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcggcacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taaggggtaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgactag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggtt aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctaggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga cgctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agcccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg
    12601 tggcagaaga tcttggcggg atgaaaaacc ttgac
//
LOCUS       DAJVPP010000108        12398 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVPP010000108 DAJVPP010000000
VERSION     DAJVPP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.108"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            171..2018
                     /gene="ldtD"
                     /locus_tag="OQM34_003262"
     CDS             171..2018
                     /gene="ldtD"
                     /locus_tag="OQM34_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1453593.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2199..2747
                     /locus_tag="OQM34_003263"
     CDS             2199..2747
                     /locus_tag="OQM34_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1453594.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2774..3421
                     /gene="gloC"
                     /locus_tag="OQM34_003264"
     CDS             2774..3421
                     /gene="gloC"
                     /locus_tag="OQM34_003264"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1453595.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3644..4834)
                     /gene="aspC"
                     /locus_tag="OQM34_003265"
     CDS             complement(3644..4834)
                     /gene="aspC"
                     /locus_tag="OQM34_003265"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1453596.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5019..6107)
                     /gene="ompF"
                     /locus_tag="OQM34_003266"
     CDS             complement(5019..6107)
                     /gene="ompF"
                     /locus_tag="OQM34_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1453597.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6708..8108)
                     /gene="asnS"
                     /locus_tag="OQM34_003267"
     CDS             complement(6708..8108)
                     /gene="asnS"
                     /locus_tag="OQM34_003267"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1453598.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8277..9479)
                     /gene="pncB"
                     /locus_tag="OQM34_003268"
     CDS             complement(8277..9479)
                     /gene="pncB"
                     /locus_tag="OQM34_003268"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1453599.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9745..12360
                     /gene="pepN"
                     /locus_tag="OQM34_003269"
     CDS             9745..12360
                     /gene="pepN"
                     /locus_tag="OQM34_003269"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1453600.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
BASE COUNT         3165 a         3080 c         3157 g         2996 t
ORIGIN      
        1 aacttcttta cattaggtta tgtaaaaacg tatcggcgtt tatatactga agataagcct
       61 gatgagtaac aggcttgctc gtcatacttt cgtgagtatt ggcgttgtac aggcaagtcg
      121 taaaataaca gcctggctat tcagagtatg ataaaaacag ggggcaaggg atgttgctta
      181 atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg cctggccgta acattcgctc
      241 cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc tggcgacagc ccggtggctg
      301 tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac ggcaataatg gcggggatcc
      361 agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca aatcgaatct caattacccg
      421 caggttacaa gccggtttat cttaaccagc ttcaactgtt gtatgccgca cgcgatatgc
      481 aacccatgtg ggaaaaccgt gatgctgtta aagccttcca gcaacagctg gcagaggtgg
      541 cgattgctgg tttccagccg cagtttaata aatgggtaga gttactgacc gatcctggtg
      601 ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat gatgggctat ctccatttca
      661 ttgcaaatat tccgatcaaa ggcactcgct ggctatatag cagtaaacct tatgcgcttt
      721 caacgccgcc gctttcggtg attaaccaat ggcagctagc gctggataaa ggtcaattgc
      781 ctacgtttgt tgcaggactg gcaccgcagc atccgcaata tgcggcgatg catgaatcgt
      841 tactggcctt actcagtgac accaaaccgt ggccccaact gaccggcaaa gcaacgttgc
      901 gcccagggca gtggagtaac gacgtaccgg cgttgcgcga aatattgcaa cgcacaggca
      961 tgttggacgg ggggccgaaa attactctac ctggcgatga cacgccaact gacgcggtag
     1021 tcagcccatc cgctgttact gttgaaacag cagaaactaa gccgatggac aagcaaacga
     1081 cgtctcgtag taaacctgcg cctgccgttc gtgccgccta cgataatgaa ctggtggaag
     1141 ccgttaaacg ttttcaggca tggcaaggat tgggagcaga tggtgctatt ggcccggcaa
     1201 cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg cgtgttggca ctcaacatcc
     1261 agcgattgcg cttgctgcca acagagcttt ctaccgggat catggttaac attccagcct
     1321 attcgctgct ctactatcag aacggcaatc aggtgctgga ttcgcgagtc attgtcggtc
     1381 gccccgatcg caaaacgccg atgatgagca gtgcacttaa caacgtagtg gtaaacccgc
     1441 cgtggaacgt accaccaact ctggcacgca aagatattct gccgaaagtg cgcaacgatc
     1501 cgggatatct cgaaagccat ggctatacgg tgatgcgcgg ctggaacagc agagaagcga
     1561 ttgacccatg ggaggttgac tggtctacaa tcacggcctc gaatttaccg tttcgcttcc
     1621 agcaggctcc aggcccacgg aactcgctgg ggcgctataa attcaatatg ccgagttcag
     1681 aggccattta tttgcatgac acgccgaacc acaatctgtt caagcgtgat acacgcgcat
     1741 tgagctcagg ctgtgtacga gtgaataaag cttccgatct ggcgaatatg ctgttgcagg
     1801 atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa gcagggtgat acacgttacg
     1861 tcaatattcg gcagtcgatt ccggtgaatc tctactacct gacggccttt gttggtgcag
     1921 atgatcgtac ccagtatcgt acagatattt acaattatga tctgcctgcg cgatccagct
     1981 cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat gaagtagttc ggggaaatca
     2041 attgtcgtaa taattagcat gattgggggc gattctctgc agcccccgtc actgctgggg
     2101 ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg cctcttgtgc gccagaagtg
     2161 catataaacg ataacattga gctgtagact tgattatcat ggacaaattc gacgctaatc
     2221 gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc cgccatcctg ccgacccctg
     2281 cgtttgcaac actctctacc ccacgcccgc gcattttgac actcaataat cttcataccg
     2341 gagagtcaat caaagcggag tttttcgatg gcagaggcta tattcaggaa gaattggcaa
     2401 aacttaacca ttttttccgc gattaccgcg cgaacaaaat aaagtccatc gacccaggat
     2461 tattcgacca gttgtatcgc ctgcaagggt tgttaggcac gcgcaaaccg gtgcaactca
     2521 tttccggtta tcgttccatt gataccaaca atgaactacg cgcccgcagc cgtggagtag
     2581 cgaagaaaag ctatcacact aaaggccagg cgatggattt ccatattgaa ggtatcgcgt
     2641 taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg tggtgtagga tattacccac
     2701 gtagtaactt tgtgcatatt gataccgggc cagcacggca ctggtagtaa tcgcttaacg
     2761 aaacaggggc agtatgaact atcgtattat tccggtcacc gcattctccc agaactgttc
     2821 attgatctgg tgtgaacaaa cccgcctggc cgcactggtc gatcctggcg gcgatgcgga
     2881 aaaaatcaaa caggaagttg atgacagcgg cctgacactg atgcagatcc tgctgacgca
     2941 tggtcatctg gaccacgttg gcgcagcggc ggaactggcg caacattacg gcgtgccggt
     3001 ttttggcccg gaaaaagaag atgagttctg gctgcaaggc ttgcctgcgc aaagtcgtat
     3061 gtttggtctg gaagagtgcc agccgctgac gccagatcgt tggctgaacg aaggcgatac
     3121 catcagcata gggaatgtga ctttacaggt gttacattgc cctgggcata cgccgggtca
     3181 tgtcgtgttt tttgatgatc gggcaaagct gctgatttct ggcgatgtta ttttcaaagg
     3241 cggagtaggg cgcagtgact tcccgcgtgg cgatcataat caactgattt cttcaatcaa
     3301 agataaattg ctgccactgg gggatgacgt gacatttatt ccgggtcacg gaccattatc
     3361 tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa gacgaaatgc ccgtctggta
     3421 aaggcgcata aaaaagtcca cttttaatgc gggcctggat ttctggcaaa gtgcgctttg
     3481 tttatgccgg atgcggcacg agcgccttat ccggcctaca aaatcgtgca aattcaaaat
     3541 attgcagggg acgcgtaggc ctgataagcg tagcgcatca ggcaatgttg cgtttgtcat
     3601 cagtctcagc ccgcttttca gcgggcttca ttgtttttaa cgcttacagc actgccacaa
     3661 tcgattcgca cagcggagcc atgttatctg gtgtcatccc ggccacattt acgcgaccag
     3721 aagcaacagc atatacgcca aactcttcgc gcagacgcag cacttgttct tttgtcagac
     3781 cactgaagga gaacatgccg ttctgtttga tgataaagct gaagtcgcgg tttgcacctt
     3841 tttcctgcag cgtattgacg aacaactgac gcatacgctg aatacgctgg cgcatatcag
     3901 tcagctcttg ttcccaaatc gcacgtaatg catcgttgct caggatggtg gcaacaacag
     3961 aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat cgccgctttc atttggctga
     4021 atgcgcgatc aacgatttca ctgtcggcag caaccagagt acaagcgcca acacgctcgt
     4081 tgtacaggcc aaagttttta gagtaggaac tggcaacaat cagctcttta tgcatagccg
     4141 cgaaagcgcg cagtccttca gcatcttctt ccagaccacg ggcaaaaccc tggtaagcga
     4201 agtcaaacag cggtaaccag cctttctcaa cggagagttg tgccagtgtc tgccattgtt
     4261 ccagcgtagg gtcgataccg gttgggttat ggcagcagcc atggaacagc actacgtcgc
     4321 cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa gtcaagggtg tgattttccg
     4381 catcataata agcgtattca cgaacttcca gacctgcaga gttaaagacg ctcttatggt
     4441 tcggccagct tgggttgctc acccacacac gcttaacgct ggtatttttt gccaggaagt
     4501 cggcagccac gcgtagtgcg ccagtgccac ccggagtctg tgccgtgcga gcacgtttgt
     4561 cattgatcag ggcgctacct ttaccaaaca gcagttcctg agtgcagcga ccaaattcaa
     4621 ggatgccgtc aatgccgagg taatttttgg tggtttcatt ttcgagcaga tactgttcag
     4681 cctttttcac gctggtcagt accggggttt tgcccgtctc atctttatag acaccaatcc
     4741 cgaggttaat tttgccggga cgttcatcgg cacgaaacag atcggccagg cccagaatcg
     4801 ggtcggcagg agcggcggta atgttctcaa acatgacgag gttccattat ggttacagaa
     4861 gggaagtccg ctatcagggt aacgggagat ttacaaaatt ccaactatta ctgatgaaaa
     4921 cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg cataaaaaaa acaggaccaa
     4981 cgtcctgttt tttcggcatt taacaaagag gtgtgctatt agaactggta aacgataccc
     5041 acagcaacgg tgtcgtctga acctacgccc agtttgttgt cagaatcgat ctggttgatg
     5101 atgtagtcaa cataggtgga catgtttttg ttgaagtagt aggttgcgcc cacttcaaag
     5161 tagttcacca gatcaacatc accgatacct tctacgtctt tcgctttaga tttggtgtaa
     5221 gcgatggacg gacgcagacc gaaatcgaac tggtattgcg caactaacag aacgtcttgc
     5281 gttttgttgg cgaagccgct ggtgtttgta aatttattag tgatcggcgt agcgttacgg
     5341 gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt cgtacttcag accagtagcc
     5401 cactgttcag cttttttacc gttgccaaga ggttgagctt cttgcaggtt ggtacggtca
     5461 gctgcaccat aagcaccaac gataccaaag ccttcgtatt cgtagctgat agaaccgcca
     5521 acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt ttttacccag gtactgaaca
     5581 gcgaagttca ggccatcaac cagaccaaag aagttggagt tacgataggt agcaacgccg
     5641 ccaacacgac caacgaagaa gtcatcgctg tatgcagtat caccaccaaa ttctggcagc
     5701 atatcggtgt aacccagtgc atcataaacc acaccgtagt tacggccgta atcgaaagaa
     5761 ccaacgtcag cgtatttaag acccgcgaat gccagacgcg ttttgttacc agtttgagcg
     5821 tcagcgcctt cagagttgtt accctggaag ttatattccc actgaccata accggtcaga
     5881 tcggaattga tttgagtttc ccctttaaaa ccaagacggg cataggtcat gtcgccattg
     5941 ccaccgtaac tgttttcacc gttacccttg gaaaaataat gcagaccaac agctttaccg
     6001 tacagatcta ctttgttgcc atctttgtta tagatttctg cagcgtttgc agtacctgct
     6061 actaacagag cagggacgat cactgccaga atattgcgct tcatcattat ttattaccct
     6121 catggttttt ttatgacacc tgccactgcc gtcaataagt tctgtcaata aaaatttacg
     6181 gaactattga tgagagtttg gtgtctttat gtgtctgcag gcatctttcc attcaaacta
     6241 acgtttcgct accgtgaaag tgctacaaag ataaagattt agtttcaaaa agaaaaaata
     6301 tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg tcatatttcc atagaaacgt
     6361 gatgatattt acagcaattt ttatctattt atatgattta cttatattta aattaactaa
     6421 acggaaattt tgtttctgat ggaaacttta tcgacctggc acaaaatcct ctttcagaca
     6481 tccagaatgc caaaaaatag tatgaaaatt gtgctatcag ctaagaaaaa actaatccgc
     6541 aataagaatg cgggttattt tttgtgcgta aatgttgcaa tgtaatattc cgtaacagga
     6601 tgatcgttat ggcgggggtg caaatagatt gcttgactta acaaacagat aacttgacag
     6661 aaaggataaa aaagagccag caggcgctgg ctctttgagg aagaaaatca gaagctggcg
     6721 ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt tttgcacgcc agttacgtaa
     6781 gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg gaacagtacc gtagcgacgc
     6841 agatcgcgat accaccagta atcttcttta ttcaggccca tttccagcat acgctcgtcc
     6901 agaacgtcca gacgttcttc acgctgggag ccaccaatga tctcaccgat gcccggagcc
     6961 agaacgtcca tagccgcaac ggttttaccg tcttcgttga ggcgcatata gaacgcttta
     7021 atatctttcg gatagttttt aaccactacc ggtgctttaa agtgttcttc cgccagataa
     7081 cgctcatgct cagaagagag atcgacgccc cagtaaaccg ggttttcaaa cttcctgccg
     7141 cagttttcga gaatggtcac tgcgtcggtg taatccacct gcgcgaaatc ggcttcaatg
     7201 aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt cagcgaagaa tttcatgtcg
     7261 tctgcgcgtt cttcgagaac cgctttgaag acatatttca gcatggcttc agccagaccc
     7321 gcgatatcgt tcaggttagc aaacgccact tccggctcca gcatccagaa ttccgccagg
     7381 tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc cgaaggtata aattttggat
     7441 aatgcgcaag cgtaggtttc gccgttcaac tggccagata cggtcaggaa agactcttta
     7501 ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt tacgcggcag gttttccaga
     7561 tccagcgtag aaacgcggaa catttcgcct gcaccttcgg tatcagatgc ggtaatcagt
     7621 ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc gatgcagcgc ctgcgccagc
     7681 gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc gcggacgcag gtgagcgact
     7741 tcacgcagat actcaatgct gtggcgtttt gccgccatcg ggtaagtgtc tggatcttca
     7801 acccaaccag caacttcaac cttgctggcc tgaatttcaa attgttgccc ctggcccggc
     7861 gatgccacga ctttacccgt cacaatgacc gagcagccgg tggtcagacg caggacgtct
     7921 tcattgtaat tgggcagaga attattgatg acagcctgta caggatcaaa gcaggaaccg
     7981 tcataaacgg cgaggaagga gatgccagct tttgaatctc ggcgggtacg tacccatccg
     8041 cgtacggtga cttcgctgtc aacggctacg cggccctgga gtacgtcggc tacaggcaca
     8101 acgctcataa tattctctct gttaatagtc ggaaaaaata aacacttgtc cacccgtaaa
     8161 tgggggggat acctatgtta cctggcatct gcaatcagac aagcagaatt cgcaaatgca
     8221 gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat tagctccctg atgatattaa
     8281 ctggcttttt taatatgcgg aaggtcgaac gctttgcgca gcgcccgaac aaacgcttta
     8341 tcatggcaga tagttttgcc agggctgtca gaaagtttcg ccaccggttt accgttacac
     8401 tctaccaact taatcacaat attcaggggt tttacctggg ggatatcgca ggtcaggcga
     8461 gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt ggcggtatag ctcaaccgct
     8521 ttgcgtaaat ccagattgtc agagaaaacc agcgttttac tctgtggatc aattcccagc
     8581 ttttcataat gtgcaatggc tttttcaccc cattcaaccg ggtcgccaga gtcatgacgc
     8641 aggccctgat accgactagc gaactcgaca ccgaaatcac gcaggaaagc atccatagtg
     8701 atgcagtcgg ttaatgcaat gccaagttgg tcgggatact cttccagcca ggcagcaagt
     8761 gcagctcgct ggctgttggc tagatccggg ctgatttgct gatgtgcctg gaaccattcg
     8821 tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg ccagatcgta gttgctggtg
     8881 cccacgaacc aggattcctg ttgcagacgc ttaacgatgg tttcttgtac ttcgcgagaa
     8941 aaacggcgac gggtgccaaa atccatcaga tggaagcgcg acatatcaag accggcggtt
     9001 aacgccgaga agtcgactaa tttgctttcc agcgtgtcga gggcttgcgc aacgtcggcc
     9061 tgcggtgagc gatagcgatg taccatttca ctgataaccg ccagcaaagg aacttcccag
     9121 aggatgactt cacgccacgg accgcttaag cgaatatcca gcttgccatt atcgttggac
     9181 acggtgactt gttccgggtt aaagcggaac tcgcgtaacc agttaagata gtcggcctta
     9241 aagaaaggca gggcagagag ccactgatat tcatcatcct gcaggcgcag gtgctgcatc
     9301 gcctgaatct gttcacgaat agcatcggca taaataccca gcagatcgtc acctcggcaa
     9361 cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa acacggcttg ctgcatatgc
     9421 aacttataag catctgtatc cagcaacgag tgcagaacag gagaagcgaa ttgtgtcata
     9481 ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa taaacatctt caggaactgc
     9541 tggagtatac cttgtttcgc aatttattga accccgatca caccatatgc cacctttctg
     9601 gtcgatggca ttacgcggtg tatgttataa aaatgtagca ataaaggcgt ttgtacctga
     9661 aaagatgaag attctgcata gcgcgattta cgcaacagga atagactgaa caccagactc
     9721 tataaaagat gctaaaggtt atttatgact caacagccac aagccaaata ccgtcacgat
     9781 tatcgtgcgc cggattacca gattactgat attgacttga cctttgacct cgacgcgcaa
     9841 aagacggtcg ttaccgcggt cagccaggct gtccgtcatg gtgcatcaga tgctccgctt
     9901 cgtctcaacg gcgaagacct caaactggtt tctgttcata ttaatgatga gccgtggacc
     9961 gcctggaaag aagaagaaga gggcgcactg gtcatcagta atttgccgga gcgttttacg
    10021 cttaagatca ttaatgaaat aagcccggcg gcgaatacgg cgctggaagg gctttatcag
    10081 tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt tccgccatat tacgtattat
    10141 ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa ttattgccga taaaatcaaa
    10201 tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag gcgaactcga aaacggacgc
    10261 cattgggtac agtggcagga cccgttcccg aaaccgtgct acctgtttgc gctggtggca
    10321 ggcgactttg atgtactgcg cgataccttt accacgcgtt ctggtcgcga agtagcactg
    10381 gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt gggcgatgac ctcgctgaaa
    10441 aactccatga aatgggatga agaacgcttt ggcctggagt atgacctcga catctatatg
    10501 atcgtcgcgg tggatttctt caatatgggc gcaatggaga ataagggtct gaatatcttt
    10561 aactccaaat atgtgctggc ccgcaccgac accgccaccg acaaagatta cctcgatatt
    10621 gaacgcgtta tcggccatga atatttccat aactggaccg gtaaccgagt gacctgccgc
    10681 gactggttcc agctcagcct gaaagaaggt ttaaccgtct tccgcgatca ggaattcagc
    10741 tctgaccttg gttcccgcgc agttaaccgc atcaataatg tgcgcaccat gcgcggattg
    10801 cagtttgcgg aagacgccag cccgatggcg cacccaatcc gcccggacat ggtcattgag
    10861 atgaacaact tctacaccct gaccgtttac gagaagggcg cggaagtgat ccgtatgatc
    10921 cacaccctgc tgggcgaaga aaacttccag aaagggatgc aactctattt cgagcgtcat
    10981 gatggtagcg cagcgacctg tgacgacttt gtgcaggcga tggaagatgc gtcgaatgtt
    11041 gatctctccc atttccgccg ttggtacagc cagtccggta cgccggttgt gaccgtcaaa
    11101 gacgactaca atccggaaac tgagcagtac accctgacca tcagccagcg cacgccagcc
    11161 acgccggatc aggcagaaaa acagccgctg catattccgt ttgccatcga attgtatgac
    11221 aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc cggtgaattc cgtgctgaac
    11281 gtcacccagg cggaacagac ctttgttttt gataatgtct acttccagcc ggtgcctgcg
    11341 ctgctgtgcg aattctctgc gccagtgaaa ctggaatata agtggagcga tcagcaactg
    11401 accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt gggatgcggc gcaaagtttg
    11461 ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc aagggcagcc gctgtctctg
    11521 ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg atgagaagat tgatccggcg
    11581 ctggcggcag aaatcctgac gctgccttct gtcaatgaaa tggctgaact gttcgatatc
    11641 atagacccga ttgctattgc cgaagtacgc gaagcactca ctcgtactct ggcgactgaa
    11701 ctggcggatg agctgctggc tatttacaac gcgaattacc agagcgagta ccgtgttgag
    11761 catgaagata ttgcaaaacg cactctgcgt aatgcctgcc tgcgcttcct tgcttttggt
    11821 gaaacgcatc tggctgacgt gctggtgagc aagcaatacc atgaagcaaa caatatgacc
    11881 gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc tgccttgccg tgacgcgctg
    11941 atgcaggagt acgacgataa gtggcatcag gacggtctgg tgatggataa atggtttatc
    12001 ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg tgcgtggtct gttgcagcat
    12061 cgctcattta ccatgagcaa cccgaaccgt attcgttcgt tgattggtgc gtttgcgggc
    12121 agcaacccgg cagcgttcca tgccgaagat ggcagcggtt accagttcct ggtggaaatg
    12181 cttaccgacc tcaacagccg taacccgcag gtagcttcac gtctgattga accgctgatt
    12241 cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc gcgcggcgct ggaacagttg
    12301 aaagggctgg aaaatctctc tggcgatctt tacgagaaga taactaaagc actggcttga
    12361 tacttttgag ccaaaatgcc tgatgcgacg cttgacgc
//
LOCUS       DAJVPP010000109        12329 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVPP010000109 DAJVPP010000000
VERSION     DAJVPP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.109"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1570
                     /gene="maeA"
                     /locus_tag="OQM34_003270"
     CDS             <1..1570
                     /gene="maeA"
                     /locus_tag="OQM34_003270"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1453601.1"
                     /translation="GLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETL
                     FYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNH
                     NIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQ
                     RLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYR
                     NEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQ
                     REGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDV
                     VRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTE
                     GNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASET
                     LAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDN
                     FWQAEYRDYRRTSI"
     gene            1704..2714
                     /gene="adhP"
                     /locus_tag="OQM34_003271"
     CDS             1704..2714
                     /gene="adhP"
                     /locus_tag="OQM34_003271"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCS1453602.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASMAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKLVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            2860..3144
                     /locus_tag="OQM34_003272"
     CDS             2860..3144
                     /locus_tag="OQM34_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1453603.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3372..4025)
                     /gene="fdnI"
                     /locus_tag="OQM34_003273"
     CDS             complement(3372..4025)
                     /gene="fdnI"
                     /locus_tag="OQM34_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1453604.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4018..4902)
                     /gene="fdnH"
                     /locus_tag="OQM34_003274"
     CDS             complement(4018..4902)
                     /gene="fdnH"
                     /locus_tag="OQM34_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1453605.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4915..7962)
                     /gene="fdnG"
                     /locus_tag="OQM34_003275"
     CDS             complement(4915..7962)
                     /gene="fdnG"
                     /locus_tag="OQM34_003275"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7375..7377),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1453606.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8194..9075
                     /gene="yddG"
                     /locus_tag="OQM34_003276"
     CDS             8194..9075
                     /gene="yddG"
                     /locus_tag="OQM34_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1453607.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9330..10430
                     /gene="ompC"
                     /locus_tag="OQM34_003277"
     CDS             9330..10430
                     /gene="ompC"
                     /locus_tag="OQM34_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1453608.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10594..11079
                     /locus_tag="OQM34_003278"
     CDS             10594..11079
                     /locus_tag="OQM34_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1453609.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11189..12178
                     /locus_tag="OQM34_003279"
     CDS             11189..12178
                     /locus_tag="OQM34_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1453610.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3222 a         3060 c         2873 g         3174 t
ORIGIN      
        1 ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa gcggaacgag catggatcca
       61 gtatcaggga ttcaaaaccg aaatcgacaa acacatctac ctgcgtaaca tccaggacac
      121 caacgaaacc ctcttctacc gtctggtaaa caatcatctt gatgagatga tgcctgttat
      181 ttatacccca accgtcggcg cagcctgtga gcgtttttct gagatctacc gccgttcacg
      241 cggcgtgttt atctcttacc agaaccgcca caatatggac gatattctgc aaaacgtgcc
      301 gaaccataat attaaagtga ttgtggtgac tgacggtgaa cgtattctgg ggcttggtga
      361 ccagggcatc ggcgggatgg gcattccgat cggtaaactg tcgctctata ccgcctgtgg
      421 cggcatcagc ccggcgtata cccttccggt ggtgctggat gtcggaacga acaaccaaca
      481 gcggcttaac gatccgctgt atatgggctg gcgtaatccg cgtatcactg acgatgaata
      541 ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa cgctggccgg acgtgctgtt
      601 gcagtttgaa gactttgctc aaaaaaatgc gatgccgtta cttaaccgct atcgcaatga
      661 aatttgttct tttaacgatg acattcaggg cactgcggcg gtaacagtcg gcacactgat
      721 cgcagccagc cgcgcggcag gtggtcagtt aagcgagaaa aaaatcgtct tccttggcgc
      781 aggttcagcg ggatgcggca ttgccgaaat gatcatcgcc cagacccagc gcgaaggatt
      841 aagcgaggaa gcagcgcggc agaaagtctt tatggtcgat cgctttggcc tgctgacgga
      901 caagatgccg aacctgctgc ctttccagac caaactggtg cagaagcgcg aaaacctcag
      961 tgactgggat accgacagcg atgtgttgtc actgctggat gtggtgcgca atgtaaaacc
     1021 agatattctg attggcgtct caggacagac cgggctgttt acggaagaga tcatccgtga
     1081 gatgcataaa cactgtccgc gtccgatcgt gatgccgctg tctaacccga cgtctcgcgt
     1141 ggaagccaca ccgcaggaca ttatcgcctg gaccgaaggt aacgcgctgg tcgccactgg
     1201 cagcccgttt aatcctgtgg tatggaaaga taaaatctac cctatcgccc agtgtaacaa
     1261 cgcctttatt ttcccgggca tcggcctggg tgttattgct tccggcgcgt cacgtatcac
     1321 cgatgagatg ctgatgtcgg caagtgaaac gcttgctcag tattcgccgc tggtcctgaa
     1381 cggcgaaggt ctggtactac cggaactgaa agatattcag aaagtctccc gcgcaattgc
     1441 gtttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg gtcaaaacgt ctgctgaagc
     1501 tttgcaacaa gccattgacg ataatttctg gcaagccgaa taccgcgact accgccgtac
     1561 ctccatctaa gcctgcaccc ggtagtgaag gctaccgggc tatttccctc tccctttttc
     1621 agatctcatc catactgggt agtggcgaat aaatctcatt tgcctcacct gctatgcaga
     1681 acatcatccg aaaaggagga actatgaaga ctgcagttgt tacgaaggat catcatgttg
     1741 acgttacgga taaaacactg cgctcactga aacatggcga agccctgctg aaaatggagt
     1801 gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg cgattttggt gacaaaaccg
     1861 gcgtaattct gggccatgaa ggcatcggtg tggtggcaga agttggccca ggtgtcacct
     1921 cattaaaacc aggcgatcgt gccagcatgg cgtggtttta cgaaggatgc ggtcattgcg
     1981 aatactgtaa cagtggtaac gaaacgctct gccgttcagt taaaaatgcc ggatacagcg
     2041 ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga ttacgcggta aaagtgccag
     2101 atggtctgga ctcggcggcg cccagcagca ttacctgtgc gggggtcacc acctacaaag
     2161 ccgttaaact gtcaaaaatt cgtcccggtc agtggattgc tatctacggt cttggcggtc
     2221 tgggtaacct cgccctgcaa tacgcaaaga atgtctttaa cgccaaagtg atcgccattg
     2281 atgtcaatga tgagcagtta aaactggcaa ccgaaatgga tgcagattta gcgattaact
     2341 cacgcaccga agacgccgcc aaaattgtgc aggagaaaac cggtggcgct cacgctgcgg
     2401 tggtaacagc agtagctaaa gctgcgttta actcggcagt tgatgctgtc cgtgcaggcg
     2461 gtcgtgttgt ggctgtcggt ctgccgccgg agtctatgag cctggatatc ccacgtcttg
     2521 tgctggatgg cattgaagtg gtcggttcgc tggtcggcac gcgccaggat ctaaccgaag
     2581 ccttccagtt tgccgccgaa ggcaaactgg tgccgaaagt cgccctgcgt ccgttagcgg
     2641 acatcaacac catctttacc gagatggaag aaggcaaaat ccgtggccgt atggtgattg
     2701 atttccgtca ctaagaggtc tttgctgcga ctcccatgtt cgggtcgcag catcgcacac
     2761 tctccaacat gaaatggcta aatggattat tgttgcgtag gaaggaacaa taatgatagt
     2821 gttcccagag aagtaacgct actgagagta aagacactaa tgaaaatggc caatcatccc
     2881 cgcccggggg acattattca ggaatcactg gacgaactta atgtcagcct gcgcgagttt
     2941 gccagagcaa tggaaattgc gccctcaacg gcaagccgat tgctgaccgg aaaagcagct
     3001 ttgacgccag aaatggcaat taaactttcc gtggtgatcg gcagttcgcc gcaaatgtgg
     3061 ctgaatctgc aaaacgcctg gagtctggca gaggcagaaa aaacggtggt tgtgtcgcga
     3121 ttacgccgtc tggttacgca ataaaaaatg gcaccttata aagcgccatt tgaaactgat
     3181 gacaaacgca acattgcctg atgcgctacg cttatcaggc ctacacgttc acttcaatct
     3241 attgtatttg aagggttttc atgccggata aggcattcac gacgcatccg ggatgaacaa
     3301 agcccacgtt gtctacaata tgaaaatggc gccttttaca gcgccatttt cagcttaaag
     3361 ttcaaaagag attatatccc ttcttcactc tcttttttcg cttctgcctt ctcgatttca
     3421 cgataccagc gcggatggtg tttcttcgcc cagcgacggc tcaccttccc ttcgatcatc
     3481 cctttaatcg accctttcac ccagaatgcc atatacatat ggatcaggat ggcgtggatc
     3541 aggatgatac ccgcagccgc gtggatcagc aggctgtagc gaacaacctg catcgggaag
     3601 tactgcgcaa agtacggacg ccagataatc accccggtca ccagcagcac gaaaatcatg
     3661 ctcatgatcg accagaacat catcttttgc ccggcgttgt acttaccaac atccgccact
     3721 ttgtgctcat tgccttttaa tacttcgaca atgttcaaca gccacggaat atctttctta
     3781 tccgggatgt tgtgatgcac aaaacgcaca aacataaaca tcagtgcgac gaaaatcgca
     3841 atgccgaaga aggggtgcaa aatgcgtccc atctgcggcg taccgaaggt ttgcgtcagc
     3901 cattgcagcg tcgggaagaa gaacgaaatc ccggacagcg ccaccaggaa gaagcaaatc
     3961 accacggtcc agtgacaggc gcgatcaata aatttggtgc gcacaatcat tttcgactta
     4021 ctcatgatga tcctcctcgt catcgtccac ttccttattc gggccaatac cgatgtagtg
     4081 gaaaatcaac ccggcaaaag tggcaataaa gccagccgct gccagcggtt tcaacgcgcc
     4141 tttccacagg cttaccgagg tgtcgatctt cggatctttc ggcagaccgt gatacagctc
     4201 cggctgatcg gcgtgatgca gcacgtacat aacgtgcgta ccaccgaccc cttccgggtt
     4261 gtagacgcca gcatgttcgt aaccacgcgc cttcagtttc gccacgcgct gttccgccag
     4321 ctccagcatc tccttcttgg tgccgaagtg gatagccccg gtcggacagg ttttcacaca
     4381 agccggttcc tggccgacgc tgacgcgatc gacgcagagc gtgcatttat atacccggtt
     4441 atcctctttg ttgaggcgcg gaatattaaa cggacacccg gcaatgcagt aaccacagcc
     4501 gatgcagttt tccgactgga aatcgacaat cccgttagcg tactgaatga ttgcaccagc
     4561 agacgggcac gccttcaggc agcccggatc ttcacagtgc atacagccgt ctttacggat
     4621 cagccactcc agcttgccat tctgttcggt ttcgctaaag cgcatcaccg tccaggactt
     4681 ggcgctcaga tcggcggggt tatcgtaaac cccgacgcag tgccccactt catcacggat
     4741 gtcgttccac tccgaacacg ccacctgaca ggctttacag ccgatacagg tggaaacgtc
     4801 gataagtttc gcgacttctg ctttgtaatc acgcacctga gaaggcggcg tgatggagtt
     4861 agttgcggac cttttgataa tatcctgcgt ttccatagcc atctgttcgc ccccttacgc
     4921 cttctcgatg ttgactaaga acgctttata ttccggcgtt tgcgagtttg catcaccgac
     4981 attcggcgtc agagtgttag cgatataacc tttacgcgcg acaccctcaa agccccagtg
     5041 gattggaata cccaccgttt caacctgctg accgtttaca ttcagcggtt tcagacggcg
     5101 cgttaccact gccaccgcgc ggataaagcc tcgcttgctg gagacagtga cacgatcgcc
     5161 attattaatg cctttcgccg ccgcctgcgt ttcgctgatt tccacaaact gttccggctg
     5221 agcaattgcg ttgagcaacg cgtgcttggt ccaggtgtgg aagtgctcgg tcagacgata
     5281 ggtcgtaccc acatacggga actgctcttt tttacccatc cgcagcgcgt cttgttcata
     5341 cagacgaaca accgggttag acaccacgtt cggatgcagc gggttagtgc ccagcggcgt
     5401 ttcaatcggc tcgtagtgtt ccgggaacgg accttccgcc attttgttga tggcaaacag
     5461 gcgtcccatc ccttccggct gcataataaa cggcccggtt ggcgtacccg gtgcggcatt
     5521 gccgaagtcc ggaatatcgt tacccgtcca cttgctgccg ttccactgga tcagcatccg
     5581 tttcggatcc cacggtttac cgttgatatc cgccgaagca cggttgtaga gtacgcgacg
     5641 gttgagcggc cacgcccagg cccatcccag cgtattcccc agaccggacg ggtcggagtt
     5701 atcgcggttt gccatctggt taccctgctc tgtccagcta ccggtgtaga tccagcaaga
     5761 agatgcggtt gtaccgtcat cacgcagatg cgcaaagcta ctcagcaact gacctttctt
     5821 cgcaatcagc acgccattag catcatagag atcttccaac gcatagccgt tgttctcttt
     5881 agctacttcg tcagattgcg gttcgtgcgg ctgcttgtag ttccagctca tcttcatcag
     5941 cggttctacg cctttaccac cttcggcctg gtacaactcg cgcagatgat ggtagatacc
     6001 cgccagaatt tcgccgtcgt tacgcgcttc gcccggcgcg tcctgacctt tccagtgcca
     6061 ctgcaaccag cgaccggagt tagcgataga accatcctct tcagcaaagc aggtcgaagg
     6121 cagacggaat acttcagtct gaatagacgc cggatcgaca tcgttcgact cgccgtggtt
     6181 ctgccagaag gtagaggttt cagtcaccag cggatcgata accaccatgt acttcagttt
     6241 gctcaggcag ctcaccactt tgtttttatc cgggaaggac gcaaccgggt taaagccctg
     6301 gcagaaataa ccggtaactt tgccttcatc catcatgttg aaatacttga tgacgtcgta
     6361 ggtctggtcc cacttcggca gccagtcata gccccagttg ttctctttct gcgcggcatc
     6421 gccatagaaa gatttcatca ggctaacgaa gaacttcgga tagttgctcc agtagttcac
     6481 ctgatcagcc agcgtcgctt tcggcgtgtt cgcttccaga tacgactgca aatcaacctg
     6541 tttttctgac ggcagcgtca gataacctgg caggctggta gagagcagac ctaagtcagt
     6601 cagcccctga atgttggagt gaccacgcaa tgcgttcacg ccgccaccgg ccatacccat
     6661 gttaccgagc agcaactgga tcatcgccat agtacggatg ttctgcgcac ccacggtgtg
     6721 ctgcgtccag cccagcgcgt acaggaaggt ggttgtgcga tccggtgcgc tggtggaggc
     6781 cagcacttca cacactttca ggaagtcggc ttttggcgta ccacagatgt tttcaacaac
     6841 gtccggcgtg tagcgggaaa cgtgcgcttt cagcaggttc cacacacagc gcggatgagt
     6901 cagtgtttca tcgcgtttcg catagccgtt ttcatcgaac tgatagttcc aggacgattt
     6961 gtcgtactgg cgtttttcag cgtcataacc gctgaacagg ccatcttcga aagcaaaatc
     7021 atcacgcacc agtaggctgg cgttggtgta atgtttaacg tattcggcgt tgattttgtt
     7081 gttttcgatc aggtagcgca aaatgccaga caggaacgta atgtccgtac cggaacgaat
     7141 aggtgcgtaa atatccgcca cagaagcggt acgcgtaaaa cggggatcga caacgatcaa
     7201 ggttgcatcg ttgttgtttt tcgcttccat cgcccagcgg aaaccgacgg gatgcgcttc
     7261 agcagcgtta ccgcccatta ccattacgac gttagcgttt ttgatatcca cccagtggtt
     7321 ggtcatagca ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac
     7381 gcgcgcctgg ttgtctaccg ccagcatccc gagggagcgg gcaaatttct gggtcagcat
     7441 cccggtttcg ttgctggcac cggaggcaca cagcataccg gtagaaagcc aacggtttac
     7501 cgttacgccc tgctcgttct tttcaataaa gttagcgtca cggtcagctt tcatcagctt
     7561 cgcaatacgg gagaatgctt cttcccagct aatgcgctgc catttgtcag aacctggcgc
     7621 acgatatttc gggtagcgca gacggttttc actgttgacg taatccagca aaccggcccc
     7681 tttcgggcac agcgcaccac ggcttaccgg atgatccggg tcaccttcaa tgtgataaat
     7741 cgcttctctg gcgttttttg cgccatcacc caggctatac atcaatagcc cgcaacctac
     7801 ggaacagtat gtgcaggtgt tacggatctc tttagcgcgt aataatttgt agtttcgcgc
     7861 ctgagccagt gcttgcttcg gggcaaagcc caatgccgct accgttgttc cagccatacc
     7921 gcccgcgcag attttaaaaa attgtctgcg actgacgtcc attgctttcc tcttttttca
     7981 ggggtattac tgcgcgagga aactaacagt aaaattctga atctttttgc gcgaaatcaa
     8041 aaagaacacg tgtttgccac aaaataatcc gcctgcgcaa attgacctac ctcaatagcg
     8101 gtagaaaaac gcaccactgc ctgacaggcc agttaaaaaa atgctataaa attcagctta
     8161 atttttaacg gcaagagaga caaaacagcg agcatgacac gacaaaaagc aacgctcata
     8221 gggctaatag cgatcgtcct gtggagcacg atggtaggat tgattcgcgg tgtcagcgag
     8281 gggctcggcc cggtcggcgg cgcagctgct atctattcat taagcgggct gctgttaatc
     8341 ttcacggttg gatttccgcg tattcggcag attccgaaag gctatttact cgccgggagt
     8401 ctgttattcg tcagctatga aatctgtctg gcgctttcct tagggtatgc ggcgacccgt
     8461 catcaggcga ttgaagtggg tatggtgaac tatctgtggc ccagcctgac aattctcttt
     8521 gccattctgt ttaatggtca gaaaaccaac tggctgattg taccaggatt attattagcc
     8581 ctggtcggcg tctgctgggt gttaggcggt gagaatgggt tacattatga tgaaatcatc
     8641 aataatatca ccaccagccc attgagttat ttcctggcgt ttattggtgc gtttatctgg
     8701 gcagcctatt gcacagtaac gaataaatac gcacgcggat ttaacggaat taccgttttt
     8761 gtcctgctaa cgggagcatg tctgtggatt tactattttc tcacgccaca accagaaatg
     8821 gtatttagca cgcccgtcat gattaaactc atctctgcgg catttacctt aggatttgct
     8881 tatactgcat ggaatgtcgg tatattgcat ggcaatgtca ccataatggc ggtaggctcg
     8941 tattttacgc ctgtactttc ctcagcgctt gcagccgtgc tgctcagcgc cccgctgtcg
     9001 ttctcgttct ggcaaggcgc actaatggtc tgcggcggtt ccctgctctg ctggctggcg
     9061 acacgtcgtg gttaaaataa ttgccgggtc tacggcccgg cagttaatca gataaattcc
     9121 gacaatatta aaaaataaat ttcaaatacc agtaattcac cttatatctc atttcgactt
     9181 cgatcacata tcgataaata atattattct gtttttctca ttgtttttaa tcgccattct
     9241 attttaaaaa cagtatgtca tttataaatg acgccgttta aaattcgttt agaaaatacg
     9301 tttaacgata attaaaagga ttctgttaaa tgaaattaaa aatagttgcg gtggttgtaa
     9361 ctggtttgtt agctgcgaac gtagcacacg ctgccgaagt ctataacaag gatggtaata
     9421 aactcgacct ttatggcaag gttaccgctc tacgttattt tactgatgat aagcgtgacg
     9481 atggtgataa aacttatgcc cgtctcggct ttaaaggaga aacgcaaatc aatgatcaaa
     9541 tgattggttt tggtcactgg gaatatgatt ttaaaggcta taacgatgaa gccaacggct
     9601 cgcgcggcaa caagacccgt ctggcctatg ctggtttaaa aattagtgaa tttggctctc
     9661 tggactatgg tcgtaactac ggtgtcggct acgacattgg ttcatggacc gatatgttgc
     9721 cagaatttgg tggcgatacc tggagtcaga aagatgtctt catgacatac cgtaccaccg
     9781 gtctagcaac ctatcgcaac tacgatttct ttggcttaat tgaagggctg aactttgccg
     9841 cgcaatatca aggcaaaaat gaacgtactg acaacagtca tctttatggt gctgactaca
     9901 cgcgtgccaa cggtgacggt ttcggtatct cctcaactta tgtttatgat ggctttggta
     9961 tcggtgcggt atataccaaa tccgatcgga caaatgcgca ggaaagagcc gcagctaatc
    10021 ctctcaatgc ctccggtaag aatgcagaac tatgggccac aggtataaag tatgatgcca
    10081 acaacatcta ctttgcagct aattacgatg aaacattaaa catgaccacc tatggggatg
    10141 gttatatctc taacaaagca caaagttttg aagtggtggc gcaatatcaa ttcgacttcg
    10201 gcttgcgccc atcactcgct tacctgaaat cgaaaggcag agatctgggc cgctacgccg
    10261 accaggacat gattgagtat atcgacgttg gtgcgacgta tttcttcaac aaaaatatgt
    10321 cgacctatgt tgattataaa atcaacctga ttgatgaaag cgactttacc cgtgccgtag
    10381 atattcgcac cgataacatc gtcgctacgg gcattaccta tcagttctaa cgtcctctcc
    10441 tgaccagccc ttctctgggc tggtttttga tttaagtgct gctgtatgac tccttaagta
    10501 aaaatgagca attcttgata tttctggtca ttcacctcaa ttaatattat tccatatcct
    10561 ccggggacat cttactataa tatttattga tgagtgatta tgaccaatat taatacagct
    10621 tgtgtaaaaa ataatgccag ttatcaactt aataacgcat tacctaacaa agaaaccatc
    10681 tccagcaatt tttgtgaacg actagaacaa tggggtaata aatcggttaa taatggtgaa
    10741 gaaagagcaa ttgccgtgga gcgaattaaa gaagcttaca attcgaacat ggcatctctt
    10801 gatttgtcat atcttgattt gagtgaatta ccgcctatcc cctcgacagt taatacgtta
    10861 aatctggaaa acaattgtct aacttgtctt gacttagctg acaatgccag tctcgttaat
    10921 atcaacctca gcgttaataa aattaacacg ataacctttc caaaagaacc aaaactggaa
    10981 aacatttata ttgatcataa caatctggaa agtttagatt taaaaaatca gcattcattg
    11041 gttaatctgg aagcgcaaaa taatagccta aaaaaatgat atttctgata gttataaact
    11101 gaaatttctt aatcttgatt ataacaagct agcatcgctg gatgtctccc ggcaagaatc
    11161 tctgattgag ttaagtgccc atcacaatat gatcactgac cttatattac acaatcatcc
    11221 caggatgaaa acaatcactt taaacgacaa ccatattgca catttaaacg ccaaaaacac
    11281 tacaaaactg gaatatttaa acttaagcaa taacaattta ctgccaacca atgacattga
    11341 tcaactaata tcatcaaaac atctttggca tgtattagtt aacggcatca acaatgatcc
    11401 acttgcacaa atgcagtact ggactgcagt aagaaatata attgatgaca ctaatgaagt
    11461 gaccattgat ttatcaggac ttaatttaac cactcaacca ccagggctgc aaaacttcac
    11521 ctctatcaat cttgataata accaactcac acattttgat gcaaccaact acgatagact
    11581 cgtaaagcta agtctgaata gtaatgctct tgagtcaata aattttcctc aaggcagaaa
    11641 tgtaagtatt acacatatat ctatgaataa taatgctctc agaaatattg atatagatag
    11701 gctttcatca gttacttatt ttagtgcggc acataatcaa ctagagtttg tgcaattaga
    11761 atcttgcgaa tggctgcaat acctgaatct cagccataat caattaactg atattgttgc
    11821 aggtaataaa aatgaactct tactgctgga tctatcccat aataaactaa caagtttaca
    11881 caatgtctta tttcccaact tgaatacgtt acttattaac aacaatttgc tttctgaaat
    11941 taaaatattc tatagcaact tccgcaatgt tcagacatta aacgctgcta acaaccagtt
    12001 gaaatatata aatcttgatt tcctgactta tcttccatct atcaaaagtt taagactgga
    12061 caataataaa ataacccaca ttgatactaa taatacatcc gatattggaa ctttattccc
    12121 cataataaaa cagagcaaaa acttaaattt tttaaatgtt tctgggaaga acaattgacc
    12181 tactatgcag ctcatgttat ttaatttgtt ttccccagca cttaagctta atactggcct
    12241 ggcaattctt tcgcctggtg catttgaagt tcactctgac ggaatagatg cggataacga
    12301 attgtttcac tatactatta aaaaagcat
//
LOCUS       DAJVPP010000110        12067 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVPP010000110 DAJVPP010000000
VERSION     DAJVPP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.110"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..580)
                     /gene="rutR"
                     /locus_tag="OQM34_003280"
     CDS             complement(<1..580)
                     /gene="rutR"
                     /locus_tag="OQM34_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCS1453611.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVF"
     gene            868..1959
                     /gene="rutA"
                     /locus_tag="OQM34_003281"
     CDS             868..1959
                     /gene="rutA"
                     /locus_tag="OQM34_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCS1453612.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            1959..2651
                     /gene="rutB"
                     /locus_tag="OQM34_003282"
     CDS             1959..2651
                     /gene="rutB"
                     /locus_tag="OQM34_003282"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCS1453613.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2663..3049
                     /gene="rutC"
                     /locus_tag="OQM34_003283"
     CDS             2663..3049
                     /gene="rutC"
                     /locus_tag="OQM34_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS1453614.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3057..3803
                     /gene="rutD"
                     /locus_tag="OQM34_003284"
     CDS             3057..3803
                     /gene="rutD"
                     /locus_tag="OQM34_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCS1453615.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVGMQLALDYPASVTMLVSVNGWLRINAH
                     TRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPRLEAEDALALAHFQGKNNL
                     LRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSSELHAALPDSQKMVMPYGG
                     HACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            3813..4403
                     /locus_tag="OQM34_003285"
     CDS             3813..4403
                     /locus_tag="OQM34_003285"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCS1453616.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4414..4908
                     /gene="rutF"
                     /locus_tag="OQM34_003286"
     CDS             4414..4908
                     /gene="rutF"
                     /locus_tag="OQM34_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCS1453617.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            4929..6257
                     /gene="rutG"
                     /locus_tag="OQM34_003287"
     CDS             4929..6257
                     /gene="rutG"
                     /locus_tag="OQM34_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCS1453618.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFTQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6339..6446)
                     /locus_tag="OQM34_003288"
     CDS             complement(6339..6446)
                     /locus_tag="OQM34_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453619.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            6819..7415
                     /gene="wrbA"
                     /locus_tag="OQM34_003289"
     CDS             6819..7415
                     /gene="wrbA"
                     /locus_tag="OQM34_003289"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1453620.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7436..7663
                     /locus_tag="OQM34_003290"
     CDS             7436..7663
                     /locus_tag="OQM34_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCS1453621.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7701..8942)
                     /gene="agp"
                     /locus_tag="OQM34_003291"
     CDS             complement(7701..8942)
                     /gene="agp"
                     /locus_tag="OQM34_003291"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCS1453622.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9235..10491)
                     /locus_tag="OQM34_003292"
     CDS             complement(9235..10491)
                     /locus_tag="OQM34_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCS1453623.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10752..11672
                     /gene="cbpA"
                     /locus_tag="OQM34_003293"
     CDS             10752..11672
                     /gene="cbpA"
                     /locus_tag="OQM34_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1453624.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11672..11977
                     /gene="cbpM"
                     /locus_tag="OQM34_003294"
     CDS             11672..11977
                     /gene="cbpM"
                     /locus_tag="OQM34_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1453625.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
BASE COUNT         2893 a         3067 c         3041 g         3066 t
ORIGIN      
        1 aaaatacctc atcgcgcaac gtcgcgcctg tcaccgcctc cacctgaggg gcgaaatcgg
       61 cgtaatgttg agtggaagcc caaatcataa aaatcaaatg ctgcggatca atcggcgcga
      121 gtttgccgct tttgacccaa ccggcaatca gcgccgattt ctcatcaatt aatgccttca
      181 aatcgcccgt cagttcatcc attaacaacg gcgcgcctgc cagcatctcc atacagaaca
      241 ggcgcgaagc ctgcggataa tcgcgtgaga cttccagctt cagacggatg tactctttta
      301 tcgccgccag cggggcgaaa tcttcacgaa acgcttttaa cggtgccagc cagatatcga
      361 gaatctgccg cagcacggca atatacagcg cctcttttga cgggaagtaa tacagcagat
      421 tggtttttga aacacccgcc aactctgcga tctgctccag tcttgtgccg tgaaaaccga
      481 attgtgaaaa agtgtccagt gctgcgctaa gaatcgcttt tttcttcgcg cttactgcgc
      541 gcgaacgttt acccgttgtt ttcactgcgc cttgcgtcat gcgctctccc ctcttgtaga
      601 ttggtaacag gatagcaaaa cccccgcccg gcctcgacct tctgcactct catcgcgcac
      661 ggatgcatgt tttatgtgca actgttttga ccgtttagtc cactttttac cagatatttt
      721 agataacaca atgctaacac attaataaca ttcacttttt aaaaactggc atccgctttg
      781 caaacaagcc aatacgcggc tgaaaaggat atgcaggatg cagcgccccg tctgactttc
      841 actttacgag acgaagagag gttgatgatg aaaattggcg tattcgtacc tattggcaac
      901 aacggctggc tcatttcgac ccacgcgccg cagtacatgc cgacctttga actgaataaa
      961 gccattgcgc aaaaagcgga gcattaccat ttcgatttcg ccctgtcgat gatcaaactg
     1021 cgtggctttg gcggcaaaac tgagttctgg gatcacaacc ttgagtcgtt caccttgatg
     1081 gcgggtctgg cagccgtgac ctcgcgcatt cagatttacg ccactgctgc caccttaacg
     1141 ttacctccgg caatcgtcgc ccgtatggcc gcaaccatcg actccatctc tggcgggcgt
     1201 tttggcgtca acctcgtgac tggctggcaa aagcccgagt atgagcagat gggtatctgg
     1261 cctggcgatg actatttctc ccgtcgttac gactatctca ccgaatatgt tcaggtgctg
     1321 cgcgacctgt ggggcacggg gaaaagcgat tttaaaggcg attttttcac catgaatgat
     1381 tgtcgcgtca gtccgcaacc gagtatcccc atgaaagtga tctgcgccgg gcaaagcgac
     1441 gctggcatgg cgttctccgc ccagtatgcc gatttcaact tctgtttcgg caaaggcgta
     1501 aatacaccca cggctttcgc cccgaccgct gcgcggatga aacaggccac agagcaaacc
     1561 ggacgcgacg ttggctctta tgtgttgttt atggtgattg ccgatgaaac cgacgatgcc
     1621 actcgcgcca aatgggaaca ctacaaagcg ggcgcggatg aagaggcgtt aagctggcta
     1681 accgaacaaa gtcagaaaga tacccgctcc ggtactgaca ccaacgttcg tcagatggcc
     1741 gatcccactt cggcggtaaa catcaatatg gggacgttag tcggttctta cgccagtgtc
     1801 gcgcgcatgt tagatgaagt cgcaagcgtg cctggtgcag aaggcgtgct gttgaccttc
     1861 gatgattttc tgtcgggaat cgaaaccttc ggcgagcgca ttcaaccact gatgcagtgc
     1921 cgcgcccatc tctctgcgct gactcaggag gtggcatgat gacaacctta accgctcgac
     1981 cggaagccat taccttcgat ccgcagcaaa gtgcgcagat cgtggtggat atgcaaaacg
     2041 cttatgccac gccaggcggc tacttagatc tcgccgggtt tgatgtctca accactcgcc
     2101 cggtcattgc caacattcaa accgctgtga ccgcagcgcg agcggcaggg atgttgatca
     2161 tctggtttca aaatggctgg gatgaacagt atgtcgaggc tggcggcccc ggctcaccga
     2221 attttcataa atcgaacgcc ctgaaaacca tgcgtaagca gccgcagctg caggggaaat
     2281 tgctggcgaa aggctcctgg gattatcaac tggtggatga actggtgccg cagcctggcg
     2341 atattgtgct gccgaagccg cgctacagcg gtttcttcaa tacgccgctg gacagcattt
     2401 tacgcagccg tggaatacgc catctggttt tcaccggtat cgctaccaac gtctgcgtcg
     2461 aatcgacgct acgcgatggc ttttttctgg agcatttcgg cgtggtgctg gaagacgcaa
     2521 cacaccaggc ggggccggaa tttgcacaga aagccgcgtt gttcaatatc gaaacctttt
     2581 ttggctgggt cagcgacgtc gaaacgttct gcgacgcgct ttctcccacg tcctttgctc
     2641 gtatcgctta aggagtttag cgatgccaaa atccgtaatt attcccgctg gcagcagcgc
     2701 accgctggcc cccttcgttc ccggcacgct ggctgatggc gtggtgtatg tctccggtac
     2761 gctggctttt gatcaacata ataacgtgct gtttgccgat gacccaaagg cgcaaacccg
     2821 ccacgttctg gaaactatcc gcaaggtgat cgagacggcg ggtggcacga tggcggatgt
     2881 gaccttcaac agcatcttta ttaccgactg gaaaaattac gccgcgatta acgaaatcta
     2941 cgccgagttt tttccgggtg ataaaccggc gcgattctac attcagtgcg gactggtaaa
     3001 acctgacgcg ctggtggaaa tcgccacaat tgcgcatatc gccaagtgag gccgcgatga
     3061 aactttcact ctcacctccc ccttatgctg atgcacccgt agtggtgttg atttcgggtc
     3121 ttggcggtag cggcagttac tggttaccgc aactggcggt gctggagcag gagtatcagg
     3181 tagtctgtta cgaccagcgc ggcaccggca ataatcccga cacactggca gaagattaca
     3241 gtatcgccca gatggcagcg gaactgcatc aggcgctggt ggggatgcag ctggcgctgg
     3301 attatcccgc gtcggtaact atgctggtca gcgttaacgg ctggctacga ataaacgccc
     3361 atacgcgccg ctgttttcag gttcgcgaac gattactgta tagcggcggc gcgcaagcat
     3421 gggtggaagc gcagccgttg ttcctctatc ccgccgactg gatggcggcc cgcgcacctc
     3481 gcctggaggc agaagacgcg ctggcactgg cgcattttca gggcaaaaat aatttactac
     3541 gtcgacttaa cgccctcaaa cgcgctgact ttagtcacca tgcggatcgc atccgctgcc
     3601 cggtgcaaat catctgcgcc agtgatgatc tgctggtgcc aacagcatgt tccagtgaac
     3661 ttcatgccgc cctgcccgat agccagaaaa tggtgatgcc ctatggcgga cacgcctgca
     3721 acgtgaccga tcccgaaacg tttaatgctc tgttactcaa cgggcttgcc agcctgttac
     3781 atcaccgtga agccgccctg taaggaattg ctatgaacga agccgttagc ccaggtgcgc
     3841 ttagcaccct gttcaccgat gcccgcactc acaacggctg gcgggagaca cccgtcagcg
     3901 atgagacgtt acaggagctt tatgccctga tgaaatgggg gccgacatca gctaactgtt
     3961 ctccggcacg gatcgtgttt atccgcactg cagaaggaaa agaacgtctg cgcccggcgc
     4021 tttccagcgg caatctgcaa aaaaccctga ccgcgcccgt caccgctatc gtcgcctggg
     4081 acagtgaatt ttatgaacgg ttaccactac tgtttcccca cggtgatgcc cgcagttggt
     4141 ttacctccag cccacaactt gccgaagaaa cagcgtttcg caacagttcc atgcaggcgg
     4201 cttatctgat cgtcgcttgc cgtgcgctgg gactggatac cggcccgatg tcgggttttg
     4261 accgtcaaca cgttgacgac gccttttttg cgggcagcac gctgaagagc aatctgctga
     4321 ttaatatcgg ctatggcgat agcagcaaac tttttgcgcg cctgccacgt ctgtcctttg
     4381 aagaagcctg cgggctgttg taaggagcaa actatgaaca tagttgatca acaaaccttt
     4441 cgcgatgcga tgtcctgtat gggcgcggcg gtcaatatca tcaccacgga cggtccagcc
     4501 gggcgcgccg ggttcaccgc cagcgccgtc tgcagcgtga ccgacacgcc gcccacatta
     4561 ctggtgtgcc tgaatcgtgg ggcgtccgtc tggccggtat tcaatgaaaa tcgaacgctg
     4621 tgtgtaaata cactgagcgc cgggcaggag ccgctttcaa atctttttgg cggcaaaacg
     4681 cccatggaac accgctttgc cgccgcccgc tggcagacgg gtgtgaccgg atgcccgcaa
     4741 ctggaagagg ctctggtttc gtttgactgc cgtatcagcc aggtggtgag cgtcggcacc
     4801 cacgacattc tgttttgcgc catcgaagcg attcatcgtc acaccacacc ctacgggctg
     4861 gtgtggttcg atcgcagtta tcacgcgctg atgcgccctg cttgttaacc tccttaagga
     4921 gacagctgat ggcaatgttc ggttttcctc attggcagtt aaaatcgacc tctacagaga
     4981 gcggcgtggt cgcgccggat gaacgattgc cgtttactca gacggcagta atgggcgttc
     5041 aacacgcggt ggcgatgttt ggcgcgacgg tgttaatgcc gattctgatg gggctggatc
     5101 ccaatctttc cattttaatg tcggggatcg gcacgttact gtttttcttt attaccggcg
     5161 ggcgcgtccc cagttatctc ggctccagcg cggcttttgt cggcgtggtg attgccgcga
     5221 cgggctttaa cggtcagggc attaacccga atatcagcat cgcgctgggc gggattatcg
     5281 cctgtgggct ggtttatacc gtcattggcc tggtggtgat gaaaattggt acacgctgga
     5341 ttgaacgact aatgccgccg gtggtgacgg gtgcggtggt gatggcgatt ggcctgaact
     5401 tagcgcctat cgcagtgaaa agtgtatcgg cttcggcctt tgatagctgg atggcggtaa
     5461 tgacggtgct gtgtattggg ctggtggcgg tgtttacccg tggcatgatc cagcggttat
     5521 tgattctcgt aggattaatt gtcgcctgtc tgctgtatgg cgtgatgacc aacgttttag
     5581 ggctgggaaa agccgtcgat tttactctcg tcagctatgc tgcctggttc ggtctgccgc
     5641 atttttccac tcctggtttt aatggccagg cgatgatgtt gattgcgccc gtggcggtga
     5701 ttctggtggc ggaaaactta ggtcatctca aggcggtcgc cgggatgacc ggacgcaata
     5761 tggacccgta tatggggcga gcattcgtcg gtgatggtct ggcaacgatg ctttccggct
     5821 ctgtcggcgg tagcggcgtc accacctatg ctgaaaatat cggtgtgatg gccgtgacaa
     5881 aagtctactc gacgctggtg tttgttgcag ctgccgtcat cgctatgctg ttgggctttt
     5941 cgccgaaatt tggcgcgctg atccatacca ttccggccgc ggtgattggt ggtgcatcaa
     6001 ttgttgtgtt cgggctgatt gccgtcgcag gcgcaaggat atgggtacaa aaccgtgtcg
     6061 atttaagcca gaacgctaat ttgattatgg tcgcagtaac cttcgtgctg ggcgcgggtg
     6121 attttgctct cacgcttggc ggttttacgt tgggagggat cggtacagca acctttggcg
     6181 cgattttact caatgcgtta ttaagcagaa ggttggttga cgttccaccg ccagaagttg
     6241 ttcatcagga gccataaaca gaaaaccgcc gggttaccgg cggtgatgac tttttgacga
     6301 gcttaagaaa aaaatgcatt cgtcattgat tagcccggct agttgtcgct tttgccgtga
     6361 ctgctcttgc cacctttttt acctgcttct gatgctcttt cgcggtcttc tgcaaaattg
     6421 ccggaaccgc ctcgatggtt tgccattact gacctccgtc tgggtataga catcatattg
     6481 catagaatta acgcggcgta tggcggctgc catattttcg ctacgtggga ctaagcttag
     6541 tgaactgacg cggacagacc cgcgtttggt aatatcctgc aacagcttct tagcgagaat
     6601 agatatcatc tccaataatt atccataagc cgctcttatg attaaaaaaa cgcgatgaga
     6661 taaaaatgtg tcattttgcg acaaaattac gtgcttgtga aagttgttat aaatcaaata
     6721 agtggttgtg aaatttgcac tctgaaaagg acgtcttatc tttaaataag tggtagcgaa
     6781 tcgctacgga atagagataa cacgaggagt ggttagaaat ggctaaagtt ctggtgcttt
     6841 attattccat gtacggacat attgaaacga tggcacgcgc agtcgctgag ggtgcaagca
     6901 aagtggatgg cgcagaagtt gtcgttaagc gtgtaccgga aaccatgccg ccgcaattat
     6961 ttgaaaaagc aggcggtaaa acgcaaactg caccggttgc aaccccgcaa gaactggccg
     7021 attacgacgc cattattttt ggtacaccta cccgctttgg caacatgtcc ggtcaaatgc
     7081 gtaccttcct cgaccagacg ggcggcctgt gggcttccgg cgcactatac ggaaaactgg
     7141 cgagcgtctt tagttccacc ggtactggcg gcggtcagga acaaaccatc acatccacct
     7201 ggacgaccct tgcgcatcac ggcatggtga ttgtccccat tggctacgca gcgcaggaat
     7261 tatttgacgt ttcacaggtt cgcggcggta cgccgtacgg cgcaaccacc atcgcaggcg
     7321 gtgacggctc acgccagcca agccaggaag aactgtctat tgctcgttat caaggggaat
     7381 atgtcgcagg tctggcagtt aaacttaacg gctaatcttc aacaggagga tacgcatgcc
     7441 aactcaagaa gcgaaagctc atcacgtcgg tgaatgggca tctctgcgca atacgtcgcc
     7501 ggaaatagcc gaggccattt tcgaagtcgc cgggtacgac gaaaaaatgg cagaaaagat
     7561 ttgggaagaa ggtagtgatg aagtgttagt caaagccttt gctaagaccg ataaagattc
     7621 gcttttttgg ggcgaacaga ccatcgaacg taaaaacgtt taaccagcga ctcccccgct
     7681 tctcgcgggg gagttttctg ttatttcacc gcctcattca acacgctatc aaacttatcc
     7741 atcgggcaga aaccgttggc atctatcggg caaccgctta attccagcgt cacgcgctgc
     7801 gcaggtgcct gcagggttaa cgcatcggca ttacgtaact gttccgcact ctggtacaca
     7861 tattcaattt tcatcaaatc gcgattggct ttgctgtcat gccaacgctg gaaaacgatt
     7921 ttgccgccaa tcggcgtgcg ttcgttctgg tcatgcaact gatacggttt gaaatccagc
     7981 gccgttaaca gagaggctat gttggagtcg tgcccaacta acaccgtgat tttcggagca
     8041 ctggcgcgat cggtgaccag ggctttgtcg atataactga ccagcggttt cgcaacattg
     8101 cgcgccactt ccggtgaggt aaacagactg tcctggtaac cgtttttcag cttcgacaat
     8161 accttccact gctggtcaga tttgatttct ccccaggcca cctgatccat cggaaaacct
     8221 tcgtagtatt gcaaagtaaa cgcatctacc agcgagttgc cgactttcag cggcccggaa
     8281 acacctggtt cttgttgata cttggcgcta aaggtatttt tgccatccac cagcgaacac
     8341 tgttgtttct ctttacaggc aggggaatct ttatagttaa cgattttttc cagtagctgg
     8401 tagctgtcgg taagctggag tttgctgagc tctttctcca ttgccgccac cgcctgttca
     8461 ctgaatgcgg cggaatcatc ggtgatcatc gggttaaagg ttgggtccat agtgcccatt
     8521 ttttcctggt gatgcacagg aatatcacac ccagggaatg caccagtaat aaagaactgg
     8581 gcggtagcga cggtacgttg cagactattg gcataggcat aaacggtgtc cggcggcggg
     8641 cattcccccg atttcactat cccctgctct gccagccatt cacgcatgta atggcccata
     8701 tacacttcga gcacgccacc tttggtggtg agttgcccac cggggacgtc ccattcgggc
     8761 catttattcg gcgtcgactg ctccagcaca ctgccattgt tcgccagcgg cgcacgtaag
     8821 ttatggcggc tcatcatgag cacttgctgt agctgatagc cttccggtac ggtttgtgcc
     8881 tgagcatttg aagcgagtaa aactatccct gccacagttg cggcgattag cgttttgttc
     8941 attcctggca cctcttttgt tatcaatcat taaagagtgt gacagaaata tgactttttt
     9001 ccgggaacag gttcgcaaaa tggcttgttg aacgcctgtt tttctatata catcttcaaa
     9061 aacaggcaag gtttgacgca aactatgcct gattagcccc tgcctcgcgt acgccgctga
     9121 taattgcacc tgtggctaaa cagaatatct gcggcgttac caatacagag acaatattta
     9181 ccaataaaaa agtgtgagac taataacaat ggtttttagc atatgccttt gccattaagt
     9241 tgcataacga tttaaatagt caagttgcgc attcatttcc ttatttttta acttataagc
     9301 ctgagaaaaa agttcaggag ggaggtaaac atcataatga ttgccagtgt ttgttaaata
     9361 accatactca agatcttcat ctgttgaaaa ggattcattt cccaaggaga ttgatgccac
     9421 attactatta acaataaata atgagcaaat gctatctgaa agaacagcaa gaaaattctt
     9481 caccgcttta atcctatccg acgtgtcggt atctaaagca agcaaattct tgttatcatt
     9541 aaatataaac tcgggtaaat gcagtatgca ttttttgtca ttcaagcaac tatcaatgaa
     9601 ttgtttttta aaaaacagaa agctccatga cataaaacta cccacaccct gaattttgtt
     9661 gtcagattgc agatcaacgc caaataatgt ctttacatct tccctgagat tatagaatgc
     9721 cgtatcaaca agagcctcga tcgtttgttt atcaacaatt ttaataaact cttcgtatac
     9781 ttgcttttgc gtcttatggc tgcccagcgc ccatgaatac tcatgttctt ttaaaaccag
     9841 tatggctctt aacagacaat atccatcgcc atctactgga gcgtatttta atccatatgt
     9901 tttattaatt tcactctgaa taaaagatat gccagacaaa tcaataccag tatctaaatc
     9961 agatattccc ggcatattta tctgttctgg ctggatactc atattttcat catctaaaca
    10021 atactcagaa aaaacatcat cacacgcatt aatgttttca atatattcca taaagccata
    10081 ccaataataa cctttaaaat ctgtgataga ctctgaacct ctgcccggca aggacactct
    10141 aacaatatca acatcttgaa catattgtat agtatatgta atacctcctg aaacccctga
    10201 ataaatttgt gtatcattag tacgcaatgt tctcataaat aatgatagat cattatcatt
    10261 ctgcatttcc tcaaattctt tttctagtga ttcacattcc agacatggag tatcctcttc
    10321 atttacttta cgtctgagtt cattgtacaa ccgcataaaa cacaccagtg tgttttcaaa
    10381 aagcgtgatt gaatatgttt gatttttttc gtactcattt tttatatcgt tccaggcctg
    10441 tttcaaataa ttatttgcag tgcaactaat tccatgtata ttactaccca tatatagcgt
    10501 ctataaaatt taataaataa tgacgcccta gttaaactta aagtgcctgg ttcaactatc
    10561 aaaaatcgct cacccttttt cacctgttta aaatatgttc agcaacccat cttgatggcg
    10621 acctcctctc cgcgatgatt tcaataacat attctgtgtt ggcatatgaa attttgagga
    10681 ttaccctaca cttataggag ttaccttaca ggggttcctt caatttgtgt tgatttacgc
    10741 gagataacgc tatggaatta aaggattatt acgccatcat gggcgtgaaa ccgacggacg
    10801 atctcaagac aatcaagacc gcctatcgtc gactggcccg taaataccat cctgatgtca
    10861 gcaaagaacc ggatgccgaa gcccgcttca aagaggtcgc tgaagcctgg gaagtgttaa
    10921 gtgatgaaca acgtcgcgct gagtatgatc agatgtggca acatcgcaac gatccgcaat
    10981 ttaaccgtca gttccatcat ggcgacggtc agagttttaa tgccgaagat tttgacgata
    11041 tcttctcgtc aattttcggt cagcatgccc gccagagccg tcaacgcccc gccgcacgcg
    11101 gccacgatat tgaaatcgaa gtggcggtat tcctcgaaga aacgcttact gagcataagc
    11161 gtaccatcag ctataacctg ccggtttata acgcctttgg catgatcgaa caggaaatcc
    11221 cgaaaacgct gaatgtgaag atcccggcag gcgtcggcaa tggtcaacgc atccgcctga
    11281 aaggccaggg gacgccgggc gaaaacggcg gtccaaatgg cgatttgtgg ctggtgattc
    11341 atattgcgcc acatccgctg ttcgatattg tcggccagga tctggaaatt gtggtgccgg
    11401 ttagcccgtg ggaagcggcg ctgggtacta aagtcaccgt tccaacactg aaagaaagca
    11461 ttttgctgac tatcccgcca ggcagccagg ccgggcaacg attgcgcgtt aaaggcaaag
    11521 gtctggtgag caaaaaacag accggcgatc tgtatgcggt actgaaaatc gtgatgccgc
    11581 cgaaaccgga tgaaaacact gccgcgctgt ggcagcaact ggcagacgcc cagtcgtctt
    11641 ttgatccacg taaagattgg gggaaagcat aatggctaat gttacggtga cttttactat
    11701 taccgaattt tgcctgcata ccggcatctc tgaagaggag ttgaatgaaa ttgtcggttt
    11761 gggggtggtt gaaccgcgtg agatccagga aacaacctgg gtatttgacg accatgccgc
    11821 cattgtggtg caacgcgcgg tacgcctgcg tcatgaactg gctctggact ggccggggat
    11881 cgcggtggcg ctgacgttaa tggatgatat tgcgcacctg aagcaggaaa accgcctgct
    11941 acgccagcgg ctttcccggt ttgtagctca tccgtgatgt gtgtttgccg gatgtgatga
    12001 cgcatccggc aactgcgcca taatgcctga tgcgacgcta aacgcgtctt ttcaggcgca
    12061 taaacga
//
LOCUS       DAJVPP010000111        11764 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVPP010000111 DAJVPP010000000
VERSION     DAJVPP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.111"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(66..758)
                     /gene="fimC"
                     /locus_tag="OQM34_003295"
     CDS             complement(66..758)
                     /gene="fimC"
                     /locus_tag="OQM34_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1453626.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(978..1520)
                     /gene="fimA"
                     /locus_tag="OQM34_003296"
     CDS             complement(978..1520)
                     /gene="fimA"
                     /locus_tag="OQM34_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1453627.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2001..2867
                     /gene="folD"
                     /locus_tag="OQM34_003297"
     CDS             2001..2867
                     /gene="folD"
                     /locus_tag="OQM34_003297"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1453628.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            2869..3081
                     /gene="ybcJ"
                     /locus_tag="OQM34_003298"
     CDS             2869..3081
                     /gene="ybcJ"
                     /locus_tag="OQM34_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1453629.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3189..3698
                     /locus_tag="OQM34_003299"
     CDS             3189..3698
                     /locus_tag="OQM34_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1453630.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(3734..5119)
                     /gene="cysS"
                     /locus_tag="OQM34_003300"
     CDS             complement(3734..5119)
                     /gene="cysS"
                     /locus_tag="OQM34_003300"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1453631.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5293..5787
                     /gene="ppiB"
                     /locus_tag="OQM34_003301"
     CDS             5293..5787
                     /gene="ppiB"
                     /locus_tag="OQM34_003301"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1453632.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            5790..6512
                     /gene="lpxH"
                     /locus_tag="OQM34_003302"
     CDS             5790..6512
                     /gene="lpxH"
                     /locus_tag="OQM34_003302"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1453633.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            6630..7139
                     /gene="purE"
                     /locus_tag="OQM34_003303"
     CDS             6630..7139
                     /gene="purE"
                     /locus_tag="OQM34_003303"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1453634.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7136..8203
                     /gene="purK"
                     /locus_tag="OQM34_003304"
     CDS             7136..8203
                     /gene="purK"
                     /locus_tag="OQM34_003304"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1453635.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8340..9233)
                     /locus_tag="OQM34_003305"
     CDS             complement(8340..9233)
                     /locus_tag="OQM34_003305"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1453636.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPKQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9230..9877)
                     /locus_tag="OQM34_003306"
                     /pseudo
     CDS             complement(9230..9877)
                     /locus_tag="OQM34_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            9880..11112
                     /gene="allC"
                     /locus_tag="OQM34_003307"
     CDS             9880..11112
                     /gene="allC"
                     /locus_tag="OQM34_003307"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCS1453637.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11123..>11764
                     /gene="allE"
                     /locus_tag="OQM34_003308"
     CDS             11123..>11764
                     /gene="allE"
                     /locus_tag="OQM34_003308"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCS1453638.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT         2828 a         3055 c         3020 g         2861 t
ORIGIN      
        1 ctctgcggaa tatccgtagt agtgggtatt ttcataatta gctctgatat tgtcagatga
       61 ttcggttagt ttaagttcac ttctctgacc ggggttactg aaccataatc attaacggtc
      121 tgaaatttca gctttccctg cactcctgag ggtaagggaa tttgtgaact aactctgggt
      181 gccgccatag cattccccaa cttttggctg cccacttgca ggttaacgag ggtgacataa
      241 aaaggtgatg gattatgaac attgatatag ttaccggatc gcgaaaactt gagtgtgtcc
      301 ggcgcttctg cgggtaattc ttgtaattga attggacgga gaaataattt catgcgcgat
      361 aaaatcgcca gttgcaaaac attcctgccg ttcaatgcat ttttatctac cgaagggatc
      421 gtcttaacat tcatccagaa cagagactca cgatctgccg ccagcggtgg accggtgtaa
      481 ataatacgca aagtattttc gcttttgggt tcactaacaa atagtggcgg tgtaatgatg
      541 aatgactttt cttttacacc gctgctgttt tcaatccacg aattgaccag aaagagctca
      601 ttggtatggc tatttttaat ccataccgca gtctgtttag catcagcggg ataaataata
      661 cgcgtggcac ctaatgcgat ccctccggca gcatgggcgc tggccgaaat gattaaataa
      721 aatatcatga gcatcaataa ctttatttta gtcatcatag ttaatacgcc ttgtgtattc
      781 ctgacggcaa aaataaggag tataaagaaa tgattgcttt ggcttcttac aacacatcat
      841 tcccttttca tttaataaag cggcatgata caaagtgaac gtggtttcga tttacattgc
      901 caccataccg cttcagacaa ccggtacatt actttgttat aagacgtgat atataattca
      961 aaacaacgtg gttttgatta ttcgtatctc ataataaaag tcgcgtcagc atttgcttgc
     1021 cctgctgatg cacttgtacc ggtgccttta taacgtgcag aaaaatgaag aacgttggtc
     1081 ccggggatca agttctggtt ggttgagaag ctattcccat caggtttgag aattgcggat
     1141 gtattatcaa gtatttcaat accgacaccc gttgctgttg ttgtatttgt actggatgca
     1201 atggccaata aattatcatt gattgcatca gcctgaccag aaaatgcaac ggcagcatta
     1261 gcggcaacaa caggatcgca gtcgttcaac tgaatggtga atggaattaa tgcagaggta
     1321 ttaccaacag cattgaaaat atcggtacga tattgaccga gtgtgacaac ctggtctgcc
     1381 gagtcagtat tcactgaaca ggcagcattc accagttcgc cttcaaagtg aattgtaccg
     1441 ccatctacaa cggcagcata actacccgta gcggcgaata gtgcggcagc cagcgcagac
     1501 gaaataaatc ttaatttcat atatattcct tcaatctcat ttatcgactc cacatccgta
     1561 tataaccgat tactttattt aagacactga tagtagtaaa attccttttt atcctctaag
     1621 aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata gagagccccg ttagatgaat
     1681 acttccgcgc aaaatatatt caacacaaat atagacctga agcggtaaat taccaggctg
     1741 aaaattcttt ttatattgtc aggtatttct taaattatct taatccttaa acaaggaaat
     1801 aaatcagttc cagatttaca acgccatcat gtacgaaaaa tgaagctttc aatctcagcg
     1861 acctcagcga cggtgcgcct aaccttcgca agaggtcgct tcacgcgata aatctgaaac
     1921 gaaacctgac agcgcgcccc gcttctgaca aaatagacgc atccccttcg acctacgtaa
     1981 cagatggaat cctctctctg atggcagcaa agattattga cggtaaaacg attgcgcagc
     2041 aggtgcgctc tgaagttgct caaaaagttc aggctcgtat tgcagccgga ctgcgggcac
     2101 caggactggc cgttgtgctg gtgggtagta accctgcatc gcaaatttat gtcgcaagca
     2161 aacgcaaggc ttgtgaagaa gtcgggttcg tctcccgctc ttatgacctc ccggaaacca
     2221 ccagcgaagc ggagctgctg gagcttatcg atgcgctgaa tgccgacaac accatcgatg
     2281 gcattctggt tcaactgccg ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta
     2341 ttcatccgga caaagacgtg gacggtttcc atccttacaa cgtcggtcgt ctgtgccagc
     2401 gcgcgccgcg tctgcgtccc tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca
     2461 acattgatac cttcggcctc aacgccgtgg tgattggcgc atcgaatatc gtcggtcgcc
     2521 caatgagcat ggaactgttg ctcgcaggtt gcaccaccac cgtgactcac cgcttcacta
     2581 aaaatctgcg tcatcacgta gaaaatgccg atctgttgat cgttgccgtt ggcaagccag
     2641 gctttattcc cggtgactgg atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc
     2701 gtctggaaaa tggcaaagtt gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct
     2761 catacattac gcctgttccc ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa
     2821 acacgctaca ggcgtgcgtt gaatatcatg atccacaggg tgagtaacat ggcgacattt
     2881 tctttaggta aacatccgca cgttgagctg tgcgacttgc tgaaactgga aggctggagc
     2941 gaaagcggcg cgcaggcgaa aatcgcgatt gccgaaggcc tggtgaaagt cgacggtgcg
     3001 gttgaaacgc gcaaacgctg caaaatcgtc gccggtcaga cagtgagttt tgcaggacac
     3061 agcgtacagg ttgttgcctg atccctttcc cggcctgtat caacaggccg gttctctttt
     3121 cagacttttg ccctctacgc cccgttcatc catgctgata gcatcgacat tttgccgaag
     3181 cgaccatcat gccaaccgtt attacgcacg ccgccgttcc cctttgcatt ggtttagggt
     3241 tgggttcgaa aggtctgtta tttgccggaa tcatcctggc gatgctgccc gatgccgacg
     3301 tattgtcgtt taaatttggc gttgcttacg gcaatgtttt tggtcatcgc ggttttaccc
     3361 attcgctggt gtttgcgttt gttgtcccgc tcttatgtgt gctgattgga cgtcgatggt
     3421 tcagggctgg gctgattcgc tgctggctgt ttttaaccgt ctcgttgcta tcgcacagct
     3481 tactggattc ggtaaccact ggcggtaagg gcgttggctg gctgtggccg tggtcagatg
     3541 aacgcttttt cgctccctgg caggtgatta aagtcgcgcc gtttgcattg tctcgttaca
     3601 ccacagcgta cgggcatcag gtgattattt ccgaattgat gtgggtgtgg ttgccgggga
     3661 tgctactgat gggaatgttg tggtggcgca gacgataacc ggatgcgaaa actcgcatcc
     3721 ggcaattgcg caattactta cgacgccagg tggtcccttg cgggccatct tccagcacga
     3781 tccccatctc gttaagacgg tcacgtgccg catctgccgc cgcccagtct ttcgctttac
     3841 gggcatccag acgctgttga attaacgctt caatctcagc cacttcgctg tcgtctgcct
     3901 gcgcgccgct ttgcaggaac gcttccggtt cttgctccag caggcccaat acagcggaaa
     3961 gtttacgcag gtgagatgcc attgcattcg ccgctgccat atcttctgct ttcagacggt
     4021 tcacttcacg cgccatatca aacagcacgg aataggcttc cggggtgttg aaatcgtcgt
     4081 ccatcgcttc aataaagcgc gcttcaaacg cttcgccacc ggcaggcgca acggttttat
     4141 ctgtgccgcg cagcgcagtg tagaggcgct ccagcgccgc acgcgcctgc ttcaggttct
     4201 cttcgctgta gttcagctgg ctgcgatagt ggcccgacat caggaagtaa cgcacggttt
     4261 ccgcgtcgta gtatttcagc acatcgcgca cggtaaagaa gttacccagc gatttggaca
     4321 tcttctcgcg gtcaaccatc accattccgg agtgcatcca gtagttcaca tactgaccat
     4381 catgggcaca ggtggactgc gcgatttcgt tctcgtggtg cgggaacatc aggtctgaac
     4441 cgccgccgtg gatatcaaag tggttaccca gctgcttgca gttcatggcc gaacattcaa
     4501 tgtgccagcc aggacgaccc gcgccccacg gagacggcca gctcggttcg ccctctttcg
     4561 acatcttcca cagaacgaag tccatcgggt tgcgtttgtc atcaaccacg tcaacgcgcg
     4621 cgccagcctg cagctggtcg agatcctgac gcgacagcac gccataagtt ggatcggtcg
     4681 ggacgtcgaa catcacgtcg ccgttgtccg ccacataagc gtgacctttg gcgatcagtt
     4741 gttcagtgag ttcaataatt tctgcgatat ggtgcgtcgc gcgcggctcc atatccgggc
     4801 gcagaatgtt caaagcatca aaatctttgt gcatttcggc gatcatgcga tccaccagcg
     4861 ccacaaagct ttcgccattt tcattggcgc gtttgatgat tttgtcgtcg atatcggtaa
     4921 tgttgcgcac atacttcagt ttatagccga ggaaacgcag atagcgcgca accacgtcaa
     4981 aagcaacaaa ggtacgcccg tgaccgatat gacagagatc gtaaacggtg attccacaca
     5041 cgtacatgcc gacttccccg gcgtgaatag gcttaaattc ctctttttgg cgtgtcagag
     5101 tattgaagat ttttagcatc gaagattccg tttagacatg tgtgggtaat tgagttgcgt
     5161 ataataccca tatttcccgc gcgaatcagc atacattgcg tgatgatccg atcgtgcggt
     5221 tatgctataa caccacccta tatatgaccc gaactgggtt gaagcaccaa tcaaacggaa
     5281 caggatgcaa aaatggttac tttccacacc aatcacggcg atattgtcat caaaactttt
     5341 gacgataaag cacctgaaac agttaaaaac ttcctggact actgccgcga aggtttttac
     5401 aacaacacca ttttccaccg tgttatcaac ggctttatga ttcagggcgg cggttttgaa
     5461 ccgggcatga aacaaaaagc caccaaagaa ccgatcaaaa acgaagccaa caacggcctg
     5521 aaaaataccc gtggtacgct ggcaatggca cgtactcagg ctccgcactc tgcaactgca
     5581 cagttcttca tcaacgtggt tgataacgac ttcctgaact tctctggcga aagcctgcaa
     5641 ggttggggct actgcgtgtt tgctgaagtg gttgacggca tggacgtggt agataaaatc
     5701 aaaggtgttg caaccggtcg taacggtatg caccaggacg tgccaaaaga agacgttatc
     5761 attgaaagcg tgaccgttag cgagtaatcg tggcgacact ctttattgca gatcttcatc
     5821 tctgcgtgga agaaccggcg atcaccgccg gttttctgcg ttttttagcg ggggaagccc
     5881 gcaaggccga cgcgctgtat attcttggcg atctgtttga agcatggatt ggcgacgacg
     5941 atcccaaccc actccatcgc cagatggcgg cagcgatcaa agcggtgtcc gattccggcg
     6001 ttccctgtta tttcattcat ggcaaccgtg attttctgct cggcaaacgc tttgcccgtg
     6061 aaagcggcat gacattattg ccggaagaaa aggtgctcga actttatggt cgccgggtgt
     6121 tgattatgca tggcgacacg ctgtgcaccg atgacgcggg ttatcaggct tttcgcgcca
     6181 aagtccacaa accctggctg caaatgctat tcctcgccct gccgttgttt gtgcgcaaac
     6241 gcattgccgc gcgaatgcgc gcgaacagca aagaagccaa cagcagtaaa tcgctggcga
     6301 tcatggacgt taaccaaaac gctgtggtca gtgcgatgga aaaacatcaa gtgcaatggc
     6361 taatccacgg gcatacccat cgcccggcgg tgcatgaact tatcgccaac cagcaacctg
     6421 cttttcgcgt ggtactgggt gcctggcata cggaaggttc gatggtgaaa gtcacggctg
     6481 atgacgttga gctgattcat tttccgtttt aaaaaaccca caactttgct gatttcacag
     6541 ccacgcaacc gttttccttg ctctctttcc gtgctattct ctgtgccctc taaagccgag
     6601 agttgtgcac cacaggagtt ttaagacgca tgtcttcccg caataatccg gcgcgtgtcg
     6661 ccatcgtgat ggggtccaaa agcgactggg ctaccatgca gttcgccgtc gaaatcttcg
     6721 aaatcctgaa tgtcccgcac cacgttgaag tggtttctgc tcaccgcacc cccgataaac
     6781 tgttcagctt cgccgaaagc gccgaagaga acggttatca ggtgattatt gcgggcgcag
     6841 gcggcgcagc gcacctgcca ggcatgattg ccgccaaaac gctggtgccg gtgctgggcg
     6901 tgccagtaca gagcgccgca ctgagcggtg tcgatagcct ctactccatc gtacaaatgc
     6961 cgcgcggcat tccggtgggt acgctggcga ttggtaaagc tggcgcggca aacgcggcat
     7021 tactggcagc acaaattctc gcgactcacg ataaagagct acaccagcgt ctgaatggct
     7081 ggcgcaaagc ccagaccgac gaagtgctgg aaaacccgga cccgcgaggt gcggcatgaa
     7141 acaggtttgc gtcctcggta acgggcagtt aggccgtatg ctgcgtcagg caggcgaacc
     7201 gttaggcatt gctgtctggc cggtcgggct ggacgctgaa ccggcggcgg tgccttttca
     7261 acaaagcgtg attaccgctg agatcgaacg ctggccggaa accgcattaa cccgcgagct
     7321 ggcgcgtcat ccggcctttg tgaaccgcga tgtgttcccg attattgctg accgtctgac
     7381 tcagaaacag cttttcgata agctccacct gccgaccgcg ccgtggcagt tacttgccga
     7441 tcgcagcgag tggcctgcgg tgtttgatcg tttaggtgaa ctggcgattg ttaagcgtcg
     7501 cactggtggc tatgacggtc gcggtcaatg gagtttacgc gccaatgaaa ccgaacagtt
     7561 accggcagag tgttacggtg aatgtattgt cgagcagggc attaacttct ctggtgaagt
     7621 atcgctggtt ggcgcgcgcg gctttgatgg cagcaccgtg ttttatccgc tgacgcataa
     7681 cctgcatcag gacggtattt tgcgcaccag cgtcgctttt ccgcaggcca acgcacagca
     7741 gcaggcgcaa gccgaagaga tgctgtcggc gattatgcag gagctgggct atgtgggcgt
     7801 gatggcgatg gagtgttttg tcaccccgca aggtctgctg atcaacgaac tggctccgcg
     7861 tgtgcataac agcggtcact ggacacaaaa cggtgccagc atcagccagt ttgagctgca
     7921 tctgcgggcg attaccgatc tgccgttacc gcaaccagta gtgaataatc cgtcggtgat
     7981 gatcaatctg attggtagcg atgtgaatta tgactggctg aaattgccgc tggtgcatct
     8041 gcactggtac gacaaagaag tccgtccggg gcgtaaagtg gggcatctga atttgaccga
     8101 cagcgacaca tcgcgtctga ctgcgacgct ggaagcctta atcccgctgc tgccgccaga
     8161 gtatgccagc ggcgtgattt gggcgcagag taagttcagt taagcaggtg ctcatttgat
     8221 tgccggatgc ggcgtaaacg ccttatccgg cttaccgctc cggtacccat tgtaggcctg
     8281 ataagacgcg tcaagcgtcg catcaggcat ggtgcaccga ttgccggatg ctctcatcac
     8341 tacagcgaaa tacaggtccc cgcttcgccc gccagcgtct cttcaattcg cgataacgcc
     8401 ccaatccacg cgggtttact acggcttctg acataaccac tcaccgccgt taccttcggc
     8461 cccatcgaac catcggcttt ggcaaatggc gctaactcat ccggtgtggc atggcgaatg
     8521 gcacgttgct tcggcgttcc ccagttttca tataccgcat cagcatcggt gaggatcacc
     8581 agtccatcgg cattaatctg ctcggcgagc aacgcagcgg cgagatcttt atcaatcacc
     8641 gcttcactcc ctgcaccatc ttccgtcaca ggcacaccgc cgccgccact gcaaatcacc
     8701 acatgcccct ctttgagcaa caactcgatg gcttcgctgt cgagaatttt acgcggttgc
     8761 ggagacgcca ccacccggcg caaatattta ccatcacgtt tcatctgcca gccgtaagcc
     8821 gcttccagtg cctcttgttc ttctggctga taaaccggac caataaattt ctctggctgc
     8881 aaaaacgccg gatcatcagg cgaaacctca atgcgcgtca gcaccgtcgt aacaggcggc
     8941 atctgcggct gtgcgctcaa gctctgcgcc agcatatagc caatcatccc ctggctttcc
     9001 gccaccagta catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc
     9061 agcagcccca cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg
     9121 cgtgccagcg cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc
     9181 tcaccgcgct ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc
     9241 cttgtaataa ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc
     9301 ccagcgccag cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga
     9361 caaagtgcag gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc
     9421 tcaccgctgt ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc
     9481 gcgcatctaa agcgccataa taccaggccg ccagcagcat tccgctcaac gtgtcatcat
     9541 gacttggcgt taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat
     9601 tgagcgcggc ctgaaaacag tgacgaaact ggcgtaattc agcgggcagc ggatcgcttg
     9661 ccgccatcgc cagtggcccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg
     9721 caagcggttg aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt
     9781 taaccgtgaa acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc
     9841 ataatccacc gcataacgca tcgaattgca tgcggcgaaa ttacacattc ccgtcaagct
     9901 atagaagaaa cgctgccctg gctttcctct tttggcgctg acccaacggg tgggatgacc
     9961 cgtttacttt attcgccgga atggctggaa acccagcagc aatttaaaaa aagaatggca
    10021 gcaagtgggc tggaaacacg tttcgatgaa gtggggaatt tatacggtcg cctgagtggc
    10081 accgaatatc cacaggaagt ggttctgagc ggttcgcata tcgataccgt ggttaacggc
    10141 ggtaacctcg acgggcaatt cggcgcgctg gcggcgtggc tggcaattga ctggttgaaa
    10201 acgcaatacg gcgcaccgtt acgtacggtc gaagtggtgg cgatggcaga agaagaaggc
    10261 agccgcttcc cgtatgtctt ctggggcagt aaaaatatct ttgggctggc gaatcctgac
    10321 gacgtgcgga atatctgtga tgccaaagga aatagttttg ccgatgcgat gaaggcttgc
    10381 ggatttactc tgccgaacgc cccactaact ccgcgtcagg ctataaaagc ctttgttgaa
    10441 ctgcatattg aacagggctg tgtgctggaa agtaatgggc aatcaattgg cgtggtgaat
    10501 gcaattgtcg ggcagcgtcg ctatacggtg acgctgaacg gcgaatcaaa ccatgcaggc
    10561 accacgccga tgggttatcg tcgtgatacg gtttacgctt tcagtcgcat ttgccatcag
    10621 tcgatcgaaa aagcgaaaaa gatgggcgat ccgctggttc tgacctttgg caaagtagag
    10681 ccgcgcccga atacggtaaa tgtggtgccg ggtaaaacca cgttcaccat tgattgtcgt
    10741 cataccgacg ctgccgtgct gcgcgatttc acccaacagt tagaaaacga catgcgggcg
    10801 atttgcgatg aaatggacat tggtattgat atcgatttat ggatggacga agaacccgtg
    10861 ccgatgaata aggagctggt cgccaccctg acagaattgt gtgaaagaga aaaactgaat
    10921 taccgggtga tgcacagtgg tgccgggcac gacgcgcaaa ttttcgcgcc tcgcgtacca
    10981 acctgcatga tttttatccc cagcatcaac gggatcagcc ataacccggc ggaacgcacc
    11041 aatattactg accttgccga aggggtcaaa acgttggcac tcatgcttta tcaacttgcc
    11101 tggcagaaat aaggagtcat aaatgggata tttaaataac gtcaccggtt accgcgaaga
    11161 tttactggct aaccgtgcga ttgttaaaca cggtaatttc gcactgttaa ccccggatgg
    11221 tctgataaaa aatattattc cggactttga aaattgtgac gcgacaatcc tctccacgcc
    11281 aaagctgggt gcctcttttg ttgattatct ggtcacactg catcaaaacg gtggcaacca
    11341 acagggcttc ggtggcgaag gcattgaaac gttcctgtat gtgatctctg gaaatatcac
    11401 tgccaaagcc gaaggcaaaa catttgcctt aagcgaaggt ggctatcttt attgcccacc
    11461 aggctcctta atgacgtttg ttaacgccca ggccgaagac agccaaatct ttttatataa
    11521 gcgccgctat gttccggtag aaggccatgc accgtggctg ttttctggca atgccagcga
    11581 actggaacgc attcactatg aaggtatgga tgatgtgatc ctgctggatt ttctgcccaa
    11641 agagttaggt tttgatatga acatgcatat cctctctttt gcaccaggtg ccagccacgg
    11701 ttatatcgaa acacacgttc aggaacatgg tgcctatatt ctttccggtc agggggttta
    11761 taac
//
LOCUS       DAJVPP010000112        11742 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVPP010000112 DAJVPP010000000
VERSION     DAJVPP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.112"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            208..1305
                     /gene="mltA"
                     /locus_tag="OQM34_003309"
     CDS             208..1305
                     /gene="mltA"
                     /locus_tag="OQM34_003309"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1453639.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1544..2350
                     /gene="tcdA"
                     /locus_tag="OQM34_003310"
     CDS             1544..2350
                     /gene="tcdA"
                     /locus_tag="OQM34_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1453640.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2401..2844)
                     /gene="csdE"
                     /locus_tag="OQM34_003311"
     CDS             complement(2401..2844)
                     /gene="csdE"
                     /locus_tag="OQM34_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1453641.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2844..4049)
                     /gene="csdA"
                     /locus_tag="OQM34_003312"
     CDS             complement(2844..4049)
                     /gene="csdA"
                     /locus_tag="OQM34_003312"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1453642.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4241..4468
                     /locus_tag="OQM34_003313"
     CDS             4241..4468
                     /locus_tag="OQM34_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1453643.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4819..5736
                     /gene="gcvA"
                     /locus_tag="OQM34_003314"
     CDS             4819..5736
                     /gene="gcvA"
                     /locus_tag="OQM34_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1453644.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5755..6150
                     /locus_tag="OQM34_003315"
     CDS             5755..6150
                     /locus_tag="OQM34_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1453645.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6143..7243
                     /gene="rlmM"
                     /locus_tag="OQM34_003316"
     CDS             6143..7243
                     /gene="rlmM"
                     /locus_tag="OQM34_003316"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1453646.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7287..8018)
                     /gene="fucR"
                     /locus_tag="OQM34_003317"
     CDS             complement(7287..8018)
                     /gene="fucR"
                     /locus_tag="OQM34_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1453647.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8076..8240)
                     /gene="fucU"
                     /locus_tag="OQM34_003318"
                     /pseudo
     CDS             complement(8076..8240)
                     /gene="fucU"
                     /locus_tag="OQM34_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="catalyzes the interconversion of alpha-L-fucose to
                     beta-L-fucose; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
     gene            complement(8270..9086)
                     /gene="fucK"
                     /locus_tag="OQM34_003319"
                     /pseudo
     CDS             complement(8270..9086)
                     /gene="fucK"
                     /locus_tag="OQM34_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(9195..10970)
                     /gene="fucI"
                     /locus_tag="OQM34_003320"
     CDS             complement(9195..10970)
                     /gene="fucI"
                     /locus_tag="OQM34_003320"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCS1453648.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11003..>11742)
                     /gene="fucP"
                     /locus_tag="OQM34_003321"
     CDS             complement(11003..>11742)
                     /gene="fucP"
                     /locus_tag="OQM34_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1453649.1"
                     /translation="PEQLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSD
                     NHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAG
                     FAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIA
                     LTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNI
                     PTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         2781 a         2896 c         3173 g         2892 t
ORIGIN      
        1 ccgtggatgc gcactatact ccgctcgcgt tttcatgcaa cctttttttt accccgatca
       61 cggatttagc atgattttga acaaaaacaa accactaaag cctatttttt gtgcaaaatt
      121 tgcgtcaggg agaggctgct catttcatta aacgcggcgg tttgttatct tcgttgcgcc
      181 ttatttttta acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg
      241 ggcacggttg tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa
      301 tataaagacg ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc
      361 gcgccgatta acgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg
      421 cgtctgtatg gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc
      481 ggtgataccc gcaatatgcg ccagttcggc attgatgcct ggcagatgga aggtgccgac
      541 aactatggta acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc
      601 cgccagggcg agttccagta tcctatttac cgtatgccgc caaaacgtgg tcgtctgccg
      661 tctcgtgcgg agatctacgc cggggcattg ggcgataaat acatcctcgc ttacagcaac
      721 tctctgatgg ataacttcat tatggatgtg cagggtagtg ggtatatcga ctttggtgat
      781 ggcagtccgc ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt
      841 ggtaaggtgc tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt
      901 cgtcattggg gcgaaacaca cagtgaagcc gaggttcgcg agctgctgga acagaacccg
      961 tctttcgtct tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg
     1021 ctggttggcc gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg
     1081 ctggcagaag tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt
     1141 ctgatggtgg cgttggatgt cgggggcgca atcaaaggcc aacacttcga tatctatcaa
     1201 gggatcgggc cggaagccgg acaccgcgca ggttggtaca accactatgg acgggtttgg
     1261 gtgctgaaaa cggctccggg cgcaggtaac gtctttagcg gctgatgtga tattctgtat
     1321 gccgttttta cggatatgac agggtgaggg gtaacctcac atcctgatta ttgactaagt
     1381 gcgcgttgtt cataccggat gcggcgtgaa cgccttatcc ggcctacaaa tcatgcaaat
     1441 ccaaaatatt gcaggagtta tgtaggcctg ataagcgtag cgcatcaggc atttatgcgt
     1501 ttagggtgag ggataacctc gcccttttta attctgaggt gctatgtctg tggtaattag
     1561 tgatgcatgg cgtcagcgtt ttggtggcac agcgcgtctg tatggtgaaa aagcgttgca
     1621 actgtttgct gacgcgcata tttgtgtggt tggtatcggt ggtgtcggtt cctgggcggc
     1681 ggaagcgctg gcgcgcacgg ggattggcgc aatcacgctt atcgatatgg atgatgtgtg
     1741 cgtcaccaat accaatcggc aaattcacgc tctgcgcgat aacgtcgggc tggcaaaagc
     1801 ggaagtcatg gcggagcgca ttcgccagat taacccggag tgccgtgtaa cggtggtgga
     1861 tgatttcgtg acgccggata acgtagcgca atatatgagt gccggttatt cgtacgtgat
     1921 tgatgccatt gatagtgtac ggcccaaagc ggcgctgatt gcttattgtc ggcgcaataa
     1981 aatcccgctg gtcacgaccg gtggcgcggg tgggcagatt gacccgacgc agattcaggt
     2041 taccgatctg gcgaaaacga ttcaggatcc gctggcggcg aagttgcgcg agcgcctgaa
     2101 aagcgatttt ggcgtagtga aaaacagtaa aggtaagctc ggcgtggatt gcgtgttttc
     2161 tactgaagcg ctggtgtacc cgcagtcaga cggtacggtg tgtgcgatga aagccacggc
     2221 agaagggccg aagaggatgg attgtgcatc tggatttggc gcggcaacga tggtgaccgc
     2281 cacctttggg tttgttgcgg tttctcatgc gctgaagaag atgatggcga aagcggcgcg
     2341 tcagggttaa acaatcaggt cggatgagac gcgaacgtct tatccgaccc ggttctgtgc
     2401 ttaaacctgc ttcgtcgcag cgataatcgc ctcgcttaac gcatttaacc cctggctgcg
     2461 tgaggcgcta agctgcgcac gtaatcccag ctcatcaaac aatgccagcg gcgactgtgc
     2521 ctgtaattcg gcggcggttt tcccctcaac ggcagtcaac aacaccgcca acaggccgcg
     2581 cacaatgcgc ccttcgctgt tgccaaagaa gtgcattttg ccgttttcag ccacggtata
     2641 tcccagccag acgcggtttt cgcatccggc aatctcttta gcctgcgctt ttaactcgtc
     2701 tggcaaagcc ggaagctgtt tccccagcat gatcaactgg cgatatttat cttcccattg
     2761 cgacaacggt gcgaaggtat tgcgtaacgt ttctgcggtt acggttgtgc cgaacggatg
     2821 tccggcgaat tgcgggtttg tcattaatcc accaataatt ccagcgcgcg gtcaacggca
     2881 ttcaccagcg catccacatc actctttgta ttatatggcg caaatgaggc gcgcagtgtg
     2941 ccggttacgc ctaattctgc cagtagcggc tgagcgcaat gttgcccggc ccgcaaggca
     3001 ataccgtact ccgccagtag cgtcaccata tcgctgtgat gaacgccagc aaaatcaaag
     3061 gccagcaggc tggaatcctg gcagcggaat gaacgaaagc ctggacgttt cgccagcgct
     3121 tcttctgcaa gcgttgctaa gctacggctc cagctttcgg cctggttgat atcgtaatct
     3181 gccagccatt ccagcgccgc gcttaatcct atgacgccag cgacatttgg cgttcctgct
     3241 tccagtttcc acggcgcaga ttgagtggtg aaaccgtcaa aactcacttc gtgaatcatt
     3301 ttgccgccac ccagccaggg cgacatcgct tccagcagtt ctgatttgcc atacagcacg
     3361 ccgatacccg tcgggccata cagtttgtga ccagagaaag catagaaatc gatatccagt
     3421 tgctgaacat ccgcggggaa atgcaccgcc ccctgagcac catcaaccat caccaccatc
     3481 ccggctgaat gagcaaaggt aatcgctcgc gccagatccg ggcaaccgcc agtcacgttc
     3541 gacatctgac ccaacgccag aatccgacta cggggagtaa tcagctctgg caacaaatcg
     3601 acatccggca gtcgctgcgc attaagcggc aatttcacca ctttggctct agtttgttgg
     3661 gcgaccatca gccaggggac gaggttagcg tggtgttctg ccacgctgac aataatctca
     3721 tcgcccggtt gcagacgcgg acgcgcatag cattgtgcca ccatgttgat ggattcagtg
     3781 gtgccgcgcg tccagacgat agttttatca tccggtgcat tcagtaattg cgccactttc
     3841 tctcgtgcag cttcataacg cgcggtcagg cgttgggctt cggcaaactg gctgcgatgg
     3901 acgtttccgg cgctcaggct gtaaaactgt cgagtggctt caaccacggc ttcaggttta
     3961 agcgcggtcg cggcgctgtc gagatagacg cccgcatcct gtagtgcggg aaactgggcg
     4021 cgaaactgcg cgggattaaa aacgttcatg gtactcctcg gcttgatgca ccgatcgtga
     4081 cgcaaaattc actttgatac aaggagagtg ctaaccatca gaattatctg gatatcctgg
     4141 cgaagccgcg taagtaacat atgctgaata gtgttaggcg ttaattttag cctgttaatc
     4201 aatactatta agatttatta atcgttttaa ggagaagatg atgaaaaaga ctgccgcaat
     4261 tatttctgcc tgtatgctga cttttgccct gagcgcctgt tccggttcga actatgtgat
     4321 gcacaccaat gacggacgta ccatcgtctc tgacggcaaa ccacagactg ataacgatat
     4381 cggtatgatt tcgtataaag acgctaatgg caacaaacag cagatcaacc gtactgacgt
     4441 gaaagagatg gtcgaactgg aacagtaatt cgcgatcgca aggtaaaaaa aagcaccgca
     4501 attaggcggt gctacattaa tcactatgga cagacagggt aaatgtacag gaagtgaaaa
     4561 aaggtagctt tgctaccatg gtctgaatcg cagaccaatt gcaaacacaa caacacaaca
     4621 tcacaaccgt aagccaaaag ttcaccagaa tacgcattcc gataaaactt ttcgttccgg
     4681 ctcaggaagt gccgccacta taggtatttg ctggtagaag ctcaacggac aatttataat
     4741 ggctcagatt aaaaaaacta ataggttaca cagtgtgatc taattgttaa attcatttaa
     4801 catcaaagtt taaaagccat gtctaaacga ttaccaccgc taaatgcctt acgagttttt
     4861 gatgccgcag cacgccattt aagttttact cgcgcagcag aagagctttt tgtgacccag
     4921 gccgcagtaa gtcatcaaat caagtctctt gaggattttt tggggctaaa actgttccgc
     4981 cgccgtaatc gttcactcct gctgaccgag gaagggcaaa gctatttcct cgatatcaaa
     5041 gagatatttt cgcaattaac cgaagcgacg cgtaaactcc aggcccgtag cgccaagggg
     5101 gcgttgacgg tcagtttact ccccagtttc gccattcatt ggttggttcc gcgactttcc
     5161 agctttaatt cagcttatcc gggaattgac gttcgaatcc aggcggttga tcgtcaggaa
     5221 gataagctgg cggatgatgt tgatgtggcg atattttatg gtcggggcaa ctggccgggg
     5281 ctacgggtgg aaaaactgta cgccgaatat ttattgccgg tgtgttcgcc gctactgctg
     5341 acaggcgaaa aacccttgaa gaccccggaa gatctggcta aacatacgtt attacatgat
     5401 gcgtcacgcc gtgactggca gacatatacc cgacagttgg ggtttaatca tatcaacgtt
     5461 cagcaagggc caatttttag tcatagcgcc atggtgctgc aagcggctat tcacgggcag
     5521 ggagtggcgc tggcaaataa cgtgatggcg caatctgaaa tcgaggccgg acgtcttgtt
     5581 tgcccgttta atgatgttct ggtcagtaaa aatgcttttt atctggtttg tcatgacagc
     5641 caggcagaac tgggtaaaat agccgccttt cgccaatgga tcctggcgaa agccgctgct
     5701 gaacaagaaa aattccgctt tcgttatgaa caataattta cgtagggtac gaccatgacc
     5761 agccgtttta tgctgatttt cgccgctatt agcggcttca tttttgtggc actgggcgct
     5821 tttggtgcgc atgtgttaag taaaaccatg ggggccgttg agatgggctg gatccaaacc
     5881 ggtctcgaat accaggcgtt tcatacgctg gcgatcttag gtctggcggt ggcaatgcag
     5941 cgtcgcatca gtatctggtt ttactggagt agcgttttcc tcgcgttagg cacggtgctg
     6001 ttcagcggca gcctttattg cctggcgctg tcccatctgc gtttgtgggc gtttgtcaca
     6061 ccggttggcg gcgtgagctt ccttgcgggc tgggcgttaa tgttagttgg tgctatccgt
     6121 ttaaagcgca agggcgtaag tcatgaataa ggttgtattg ctgtgccgtc cgggctttga
     6181 aaaagagtgc gccgcagaaa ttaccgataa agccggccag cgggaaattt tcggttttgc
     6241 ccgcgtgaaa gagaatgcgg gttatgtcat ttatgaatgt tatcaacctg atgatggcga
     6301 taagttaatc cgtgagctgc cgttcagttc attaattttt gcccgccagt ggtttgtggt
     6361 gggggaactc ctgcagcatt tgccgccaga agatcgtatt acccccattg tcggcatgtt
     6421 acagggtgta gtagagaagg gcggtgaact gcgtgttgaa gttgccgata ccaacgaaag
     6481 caaagagttg ctgaaattct gccgtaagtt cactgttccg ctacgcgctg ccttgcgcga
     6541 tgctggggtg ctggcgaact atgaaacgcc gaagcgtccg gttgtgcatg tattctttat
     6601 tgcaccagga tgctgctata ccggttactc atacagcaac aataattcgc cgttctatat
     6661 gggcattccg cgcctgaaat ttccggcaga ggcgccgagt cgttccacgc tcaaactgga
     6721 agaggcattt catgtgttta ttcctgcgga tgagtgggat gaacgcctgg cgaacgggat
     6781 gtgggcggtg gatttaggcg cttgccctgg cggctggacc taccaactgg tgaagcgcaa
     6841 catgtgggtt tattccgtcg acaacggccc gatggcgcaa agtctgatgg ataccgggca
     6901 ggtgacgtgg ctgcgggaag acggtttcaa attccgtccg acacgcagca atatctcctg
     6961 gatggtatgc gatatggttg aaaaaccggc taaagttgcg gcattgatgg cgcagtggct
     7021 ggttaatggc tggtgccgtg aaaccatttt caatctcaaa ctgccgatga aaaaacgcta
     7081 cgaagaagta tcacacaatc tggcgtatat tcaggcacag cttgatgaac atggcataaa
     7141 tgctcagatt caggcgcggc agttgtatca cgatcgcgaa gaagtgacgg tgcacgtccg
     7201 ccgcatctgg gctgcggtgg gtggtcgtcg cgacgagcga taacaggcaa ggccggggcg
     7261 tcgtctccgg cctgctaccc tttccgtcag gctgttacca aagaagttgc aacctggcgc
     7321 tcatcagaaa taatgtgcgt tacctcatcc agatgcccga tgcgggcttc ccctgaacga
     7381 ttaaatttac ttttatcaat caataacaac gattgcgcgg cacgttttaa tagcatcgat
     7441 ttgtaatcag cgttgatcgc attggagtcc cacagtgcgc cgctgctatc gatcccttca
     7501 caagaaaaaa taaacagatc gatttccagc gatttaagtt gggaaatcag cgagggattg
     7561 acgtaacagc catatttgcg ctcaagtgtg ccgccggaac tgatcagttg aatgcgttcg
     7621 cgtttaccga gttcatggca aatcggatgg ctattggtga agacctgaat gttgatgtca
     7681 ggcaactggc gtgccagata ccagcaagtt gaactggcgt ctaaggctat caccatccct
     7741 tcttcaatcc acgcgagcgc ctcgcgcgcg atatctgctt tatgcgcata atggcttttc
     7801 agcctgatgt gaaagggatc gccactgtct tgattttgac ggtggatata tttagcgcgt
     7861 ccatgattgc gcagaatttt accctgcgtc tgtaattcat tgagatcgcg acgaatggtt
     7921 tctttactga cctttagctg ttcagagaga gcttccgtgg tcaggctggt atggttcagc
     7981 agcaggtcga ctatcgcttg ctggcgtgcc gctttcatat ttttcacccc atcattcgat
     8041 aaccgcccgc gtcatgcggg cgtaccggta tgagattacg gtgttacccc tttttttaaa
     8101 agaatattcc cgtacttcgc tcgttcgcct gtgataacga tcgcaaaggc tttttgcgcc
     8161 cgttcataaa acgcaaaacg attgatgcgg atgatgtcag gacacggggc ttgtagtgaa
     8221 agcgcattac ggtaacggcg tactgactta acagcgaagt atcaacctag gcgctgcgaa
     8281 ccattaaaat ttcccatgta ccggaggaga gcacgggttc attttgttcg gcaccagcgc
     8341 caaaaagggc gaactgggta tcgtgacctg cggaaatcac cggtatgcca acgggtaagc
     8401 cgagcattgc tgcggcgttg ttctgtagcg taccaatctg ttcacccgct tccaccagac
     8461 gagggaagag tcggcgtgga ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt
     8521 gctggatatc cagcatctgg ctggttccgg ccatcgtgat atcagtagtg aattcgctgg
     8581 ttaaacggtg gttaatcagc gacgaaatga agagccaggc gtgcgcgcgt tccagcagtt
     8641 gtggatgatt ttctttcaac cacaccaact tatataacgt attgaaacta aaggctccga
     8701 cgccagaaat agcctgcaac cgctgtgcgg agattaaccg ttcaatattg tccataaccg
     8761 ctgctgttcg cggacatttc cagctaatga tcggatagag cagattgcct tgcttatcta
     8821 ccagagcgcc atccacacta aaggtggtga cggcgatacc gcggatgtgg cattcagtca
     8881 gttcactatt gatttgccga cagcaatcag caaagcgttg caaaatggca gtctaaagac
     8941 cactggtgcc aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg
     9001 gcaacaattt ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg
     9061 agtaccagga taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgtt
     9121 tatccggcta accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat
     9181 cagggtgtaa tgtattaacg cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc
     9241 tggccttcaa tatccatgcc gtgcgcagcc caggcagaag gacgatacac tttggtctct
     9301 tcaacgttgt gcatacatac cgggatacgc agcatggagg caagggtgat aaagtctgcg
     9361 ccaacgtggc cgatggtcag aactccgtgg ttggcacccc agttcgccat taccgaatac
     9421 acatccgtaa acggcccttt accagtgagg cgcggtgcaa accaggtggt tggccaggtt
     9481 gagttggtgc gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct
     9541 tccgcgattt gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac
     9601 gggacgccgc cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg
     9661 atcgccgggc accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc
     9721 cagtgcggct tcatcgtcgg gttaccttcg ctgtcgcgtt gtttacagga accgtccagc
     9781 gcagcagaac ctgagttgat caaatggatg atgccgtgtt ctgccagtcc gtccagttta
     9841 tgccccgtta cacgctcaat tgcttctggt gaccagtagg tacgcacatc ggcaaatacc
     9901 tgagcggtgc cggtgagctg gtgacccatt agcattgcca cgccgttaag actgtcgttt
     9961 tcggtcgcca cgacaaaggg ttcgcgcacg ccattccagt caaatgaact gttgaggatc
    10021 gcttcggcgg tgtcaccatt gggatattga tcggtccagt gacgttgccc ctggaagccc
    10081 gcagcgatgg cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg
    10141 ttgccttgca tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg
    10201 cggctttgct cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag
    10261 tttttatcag cccaggccag tgccatttcc agttcggctt cgtcgtaaat cttctgatcg
    10321 atacggcgac gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca
    10381 aagaagttgt gatcaacaat agaaccggcg atacccatcg aaacgccgcc cagcgacaga
    10441 tagcttttac ctttcatgct ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt
    10501 tcttcaacat cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg
    10561 gagaatgccg ggatgccttt ctggctgtga gctgccagag ccgctgccag gtaaacagcg
    10621 ccggggcgtt cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatgtcg
    10681 atggtttcac tgccatagca ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg
    10741 ctgctgaatt tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa
    10801 atgacacact cgacggcagc tccgcaggca tggcgcagtt tctcggtcag cagtgcggcc
    10861 gtagctttcg ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga
    10921 ccgtcaataa ccgggcggat accaattttc ggtaagctga tttttttcat tacagattcc
    10981 tcactttatt cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga
    11041 taaagatgac cgcgaagcag agtgcgggga tcagttcagc agtggggatg ttgcccgccg
    11101 cgtcactgac aaaacccatg accggagtga caataccgcc gccaataatg gtcataacga
    11161 tgaaggacga accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg
    11221 ttgggtactg aatcgacata aaggcgctgc ataaagtcag ggctattaag cccacatgac
    11281 cgccagcgaa ggctgagatc aggcacagtg ccatagcgat taatgcgtag gcggccagga
    11341 ctttgtgtgg tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca ataaagaagc
    11401 acaccatggt tccggttaaa tagttagcgg caaagcctgc agtcatacct ggaatttctt
    11461 ctacagcgta gcgaatcaaa tagctccagc aggccgtttg tgcgccgaca tagcagaatt
    11521 gcgctaatac cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc gatgcggaga
    11581 acgatccttg tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca
    11641 tgatcagcag ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg
    11701 ataataccag gctgtgttta tacgcactca attgctctgg ag
//
LOCUS       DAJVPP010000113        11692 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVPP010000113 DAJVPP010000000
VERSION     DAJVPP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.113"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..2332)
                     /locus_tag="OQM34_003322"
     CDS             complement(<1..2332)
                     /locus_tag="OQM34_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1453650.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEEMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELV"
     gene            complement(2266..2889)
                     /locus_tag="OQM34_003323"
     CDS             complement(2266..2889)
                     /locus_tag="OQM34_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCS1453651.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2886..4574)
                     /locus_tag="OQM34_003324"
                     /pseudo
     CDS             complement(2886..4574)
                     /locus_tag="OQM34_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4723..7350)
                     /gene="gyrA"
                     /locus_tag="OQM34_003325"
     CDS             complement(4723..7350)
                     /gene="gyrA"
                     /locus_tag="OQM34_003325"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS1453652.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7497..8219
                     /locus_tag="OQM34_003326"
     CDS             7497..8219
                     /locus_tag="OQM34_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1453653.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8347..11692)
                     /gene="yfaL"
                     /locus_tag="OQM34_003327"
                     /pseudo
     CDS             complement(8347..11692)
                     /gene="yfaL"
                     /locus_tag="OQM34_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2691 a         3253 c         2903 g         2845 t
ORIGIN      
        1 tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata tcaagctgag
       61 gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg gtatacagca
      121 ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct tcatacaggg
      181 tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc gactgctgtt
      241 ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg aaagtaatca
      301 gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata tctaccgtac
      361 cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg agaattaagc
      421 cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg acggtaaagg
      481 atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga cgcaaccatt
      541 catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac acaaccgact
      601 cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg ccgcgttcaa
      661 tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg gagacggtta
      721 acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga ccgctggcgt
      781 cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa cgcaaatcgt
      841 taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca ttttttaccg
      901 gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca gttttgaact
      961 cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt gccacgcgaa
     1021 aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct ccggcaacgg
     1081 cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc gtgacatcga
     1141 cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg gcgctgacga
     1201 tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca tcgacacgat
     1261 cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg tagatttcgc
     1321 tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta cccagaatgt
     1381 atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg gtcaccccgc
     1441 gggtcatcac gccaagaccg tcagtccaca agatctctga gccgggcttc gcttcaccct
     1501 gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta agcgccacgg
     1561 tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatctcc tcgacgaggt
     1621 acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc ggcgagatga
     1681 aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt ttagcctgcc
     1741 atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa aactggttgt
     1801 tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg ggtaatgcct
     1861 gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg cgagatttgc
     1921 cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc agatattgcg
     1981 gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc gggtcaggaa
     2041 tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc cgataatcac
     2101 gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg ctgtcagcaa
     2161 gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc gaatcatcag
     2221 ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc gcgccagaaa
     2281 atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc atcgggtgtc
     2341 cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag ttttcgtggc
     2401 gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct gggcatcgcc
     2461 ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc caataaactg
     2521 gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc cgctcccctg
     2581 attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg gcggcaaata
     2641 ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca gcgtctcgtt
     2701 cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac ttggcccctg
     2761 gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg attgttcgac
     2821 attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg ccagcagccc
     2881 gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc gggttccatc
     2941 tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt cggcattaat
     3001 aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact ggtcaacgtt
     3061 tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac gatgccatca
     3121 gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag ggcgttatta
     3181 accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag cgacacacgg
     3241 aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg atactgaccg
     3301 tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt ctggcttacc
     3361 ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt aaacagtttc
     3421 cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag cggggtttgc
     3481 aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc attcagcttg
     3541 tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga atacggcacc
     3601 gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa atcgaaactg
     3661 gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc gtcgttaccc
     3721 atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa acccagcaac
     3781 ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg ctcttccagg
     3841 ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat cgccgtggct
     3901 tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac cagcatcttg
     3961 tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg ataaacagga
     4021 atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta gctgtcgctg
     4081 gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg gatcagcacc
     4141 atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc gggctgatct
     4201 aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac aatgcggcga
     4261 atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac gaaatcttcg
     4321 ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg gctcagagag
     4381 tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt gtgcaccacc
     4441 agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca gacgccagcg
     4501 gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc tttcgccttt
     4561 ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac gggcacagcc
     4621 aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga cttttcaatt
     4681 caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt cttctggctc
     4741 gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac tgccgtcgat
     4801 ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca ctacgttttc
     4861 atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga tgctgatttc
     4921 tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct ggtcgcagtc
     4981 atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga tggagataac
     5041 ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt taccgtaacc
     5101 gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag agacgacttt
     5161 atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca tcgcacggac
     5221 agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta cttcatcttc
     5281 gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga tcgccacttt
     5341 accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag taccgttagc
     5401 ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga tcgcggtgat
     5461 acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg cgccacgagt
     5521 ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg agaagcacag
     5581 aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt ctttaatacg
     5641 tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa gcggctgata
     5701 tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga tcagatcttc
     5761 caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac cgaactgttc
     5821 acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc taccaagaat
     5881 acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca gtttttcgtg
     5941 ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt gctgttcggt
     6001 caggtagtac agaccatcac gcacgccgaa ctcaggctcc agccattccg gacgcgcagc
     6061 atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg gattagcaac
     6121 cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga tgatcgggtc
     6181 aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc gagctttacg
     6241 cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa acgccgcgat
     6301 gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca tgttgatacc
     6361 gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt caccgaccgc
     6421 atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt cacgcagcgc
     6481 gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga tcaggcgcgc
     6541 tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg ttttggcgtc
     6601 aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac ggtaagcttc
     6661 ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc ccgggatgtg
     6721 ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac aaccgttgat
     6781 gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga taccggaaga
     6841 accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt tttccgtgcc
     6901 gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca gttcatgggc
     6961 aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc cgtcgatgga
     7021 accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct gcgccatacg
     7081 gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga ttacgtcacc
     7141 aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta cgttcatggc
     7201 gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca gcgcacggcc
     7261 aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct caatgttgac
     7321 cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact gtatcccgga
     7381 ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat acgctttatt
     7441 cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa gtgccaatga
     7501 atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct aaatttgaag
     7561 ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac cgcattaacc
     7621 cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa aaggtgctcg
     7681 atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc gcgacggtga
     7741 ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg ctggaaagcg
     7801 gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa catgccgggc
     7861 agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg cagtcagtgg
     7921 tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg acgcttaacc
     7981 gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg cgcatggtgc
     8041 ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt ggctgggtgg
     8101 atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg atcactaatt
     8161 cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag aataagtgag
     8221 gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat caggcctaca
     8281 aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt gatggtcaga
     8341 cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa aatcatcact
     8401 ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat tgcccgtgac
     8461 tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc tgccatcttc
     8521 ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg gtgtgacacc
     8581 aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat ccccctgcga
     8641 ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc cctgataaat
     8701 cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat acccggcttc
     8761 cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat gttcggaaac
     8821 atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct gcttctgatt
     8881 accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct ggttatcggc
     8941 gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc caccaaccgc
     9001 cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc cgctaagctg
     9061 caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat gataacgtcc
     9121 gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc gtcgctccat
     9181 cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt tggcattcag
     9241 caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat ccggcgtggg
     9301 atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt cttgcgatcg
     9361 cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca tgttgacgta
     9421 gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat cagctgcgaa
     9481 atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg agttaaccac
     9541 cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta ccgagtcatc
     9601 gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac cgtttaccgt
     9661 cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat tggtcagatc
     9721 accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt tggtattaag
     9781 ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc cgttaaatag
     9841 tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg taccatcgct
     9901 gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat ccgcgccagt
     9961 aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga tatccgcaac
    10021 atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac cgctggcggt
    10081 cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct gatgctgccc
    10141 gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa ctgccacttc
    10201 accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc gagtatcgac
    10261 attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa cctggcggtt
    10321 atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg aggcgatttg
    10381 tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc tcgcatcgac
    10441 caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc cacctgcaag
    10501 atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt caaggccatc
    10561 attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca caccgccgtt
    10621 taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt cgccttccag
    10681 cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg actgcgcccc
    10741 tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt cgatgatgcc
    10801 agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta aagtgccatt
    10861 ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt ttagctcagc
    10921 ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt gcggatcgtc
    10981 ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg taacttcacc
    11041 gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta ccagatcgcc
    11101 ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc cgtcattctc
    11161 tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt cacttaagcc
    11221 gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg ctgcgctatt
    11281 gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag aaatatcaat
    11341 aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt cggctgtgtt
    11401 atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc tgtcgctatt
    11461 attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg atgtatatgc
    11521 ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg tataaattgc
    11581 gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta aatcgacggc
    11641 accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg cg
//
LOCUS       DAJVPP010000114        11587 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVPP010000114 DAJVPP010000000
VERSION     DAJVPP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.114"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1138)
                     /gene="uxaB"
                     /locus_tag="OQM34_003328"
     CDS             complement(<1..1138)
                     /gene="uxaB"
                     /locus_tag="OQM34_003328"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS1453654.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQ"
     gene            complement(1365..2783)
                     /locus_tag="OQM34_003329"
     CDS             complement(1365..2783)
                     /locus_tag="OQM34_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1453655.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(2937..3281)
                     /locus_tag="OQM34_003330"
     CDS             complement(2937..3281)
                     /locus_tag="OQM34_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCS1453656.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3281..4207)
                     /gene="glsB"
                     /locus_tag="OQM34_003331"
     CDS             complement(3281..4207)
                     /gene="glsB"
                     /locus_tag="OQM34_003331"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1453657.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4271..5206)
                     /locus_tag="OQM34_003332"
     CDS             complement(4271..5206)
                     /locus_tag="OQM34_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCS1453658.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5286..6401
                     /gene="yneK"
                     /locus_tag="OQM34_003333"
     CDS             5286..6401
                     /gene="yneK"
                     /locus_tag="OQM34_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCS1453659.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRPLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6551..7741
                     /gene="ydeA"
                     /locus_tag="OQM34_003334"
     CDS             6551..7741
                     /gene="ydeA"
                     /locus_tag="OQM34_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1453660.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(7766..8431)
                     /gene="marC"
                     /locus_tag="OQM34_003335"
     CDS             complement(7766..8431)
                     /gene="marC"
                     /locus_tag="OQM34_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1453661.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            8643..9077
                     /gene="marR"
                     /locus_tag="OQM34_003336"
     CDS             8643..9077
                     /gene="marR"
                     /locus_tag="OQM34_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1453662.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9097..9480
                     /gene="marA"
                     /locus_tag="OQM34_003337"
     CDS             9097..9480
                     /gene="marA"
                     /locus_tag="OQM34_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS1453663.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9512..9730
                     /gene="marB"
                     /locus_tag="OQM34_003338"
     CDS             9512..9730
                     /gene="marB"
                     /locus_tag="OQM34_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1453664.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(9761..10660)
                     /gene="eamA"
                     /locus_tag="OQM34_003339"
     CDS             complement(9761..10660)
                     /gene="eamA"
                     /locus_tag="OQM34_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCS1453665.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            10924..>11587
                     /locus_tag="OQM34_003340"
     CDS             10924..>11587
                     /locus_tag="OQM34_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1453666.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTNKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEK"
BASE COUNT         3084 a         2989 c         2626 g         2888 t
ORIGIN      
        1 tctgccctgc cagcagctgc ggcaggatgc gggtgcggaa cttggtcata ccgttgagcg
       61 cgatagacag cagctgatgc ttaatgtacg ggttacggaa acgcccggtg actgcactgg
      121 cgaaagattc cagttcatca cgaggcaaat ccagtaccgg aataatttct tcgtagatgg
      181 ctttttcaac gaatgcgcaa atttcagcat cgttcatcgc ttcacctacg gtatccagcc
      241 ctgcctggaa cgccaccggc accagcgcgg tgtgcgcacc gttgaggatt gccactttgc
      301 gttctttata cggtttaata tcgtcaacaa tcagcacgtt gagcggatat ttgtccaggc
      361 gcagctcagt cgctaaggat ttcggcccct gaatcacaaa caggtaaaag tgttcagcgg
      421 tgtcgaggaa accatcgtga taacccagtt cttcttccag tttagccact tcatcgcgcg
      481 gataaccggt aacgatacgg tccaccagcg tagaacagaa gctgttagcc tgatccagcc
      541 attgaataaa tgcttctggc agtgcccact cttgcgcata gcgcagcacc agttcacgca
      601 acgcgtcgcc attgtagtca atcaactcac acggaatgat gatccaacct ttatccagcg
      661 caccgttgaa atggctaaag cgttcgaaca gcagacgggt cagttttgcc ggatagctta
      721 ccgctggcgc gtcatcgaat ttatcgcccg catggtagct gatacccgct tcggtggtgt
      781 tagagaaaac aaagcgcatt tccgggttgt gcgccagttt caggaattca tcgtattcac
      841 tgtagacgct gatttcacga ttaaccgagc gaatcagacg cgcgtcgctg accgcttccc
      901 ccttctcatt caggccacgg ataatggtgg tgtacagacc atcctgcgtg ctcagtgacg
      961 gcgggaatga agtttcaatc ggacgaacaa cgaccacgcc agaattcaga tcggtgtgct
     1021 cattcaggag atcgatttgc cagtcaacaa aggcgcgcag gaagttacct tcaccaaact
     1081 gaatgatacg ttctggatac tgtgcaccgg gaaaatcgcg acgatttagt gttttcacaa
     1141 tgggttccct tctgattagt catacaacct gtttgaattg gtgcgacagg ttagcaaact
     1201 ttaatacgcc gaacccctgt tttgatcaac tcctgatgat taatgagcag ttttatgaga
     1261 aaagtgtggc gcggatcatg gtttaatcga ggaaatacgc cttttcctgg atcataaagt
     1321 ggtagaacac attgcattca aatcgcgcga aatgaataaa gatgtcagac aacttcctca
     1381 ccgtaacgca tagtgctggt acggttgcgc ccatctttct tcgagcgata cagacacgta
     1441 tcggcttcga ccatcaattt attaaaatca tcggtcagcg ttcggtacga tgcgcgacca
     1501 ctaccgacgc caatacttac cgtgagataa agcgtttttt gttgccaggt gaatggttgc
     1561 agttcaacgc ctttacggat tttttccgcc attagcagac catcgacagg attcaccgac
     1621 ggcactgcaa cagcaaattc ttcgccgccc atgcgcgcca ccagcccctt atcaccgaca
     1681 atcttctgaa tatgccgggc aaacacgctt aacactttat cgccacattc atgtccatag
     1741 ttatcgttaa tgcttttgaa atagtcgata tcaagcagca tgaccgtcag atgttgtgtc
     1801 tgtttcagcg atggactttt cagcgcctca taaagaccgg accgtgagta aacctgagtc
     1861 agaaaatcaa agtcggctcg cagcgcaact tgcttcatta gcaaattgat cgctgccacg
     1921 ctaaaagaaa ccataattgg gcatatcgcc atcgtggcaa taccgagacg tgcggagaac
     1981 atttccggaa tggagaacgg cgaaccgacc gagatatcaa tcaccgaatt tgccaccagt
     2041 acgatttcca ccgcaccggt gacaaaggtc aacagacatg ttacctgcgg cgtatagcgc
     2101 actgcacacc agattaatgc aggcagcggg aacgccagac tccccgctcc gccaatgact
     2161 accgaggcaa taaccgacac aataagcgcg atggcgggca tcatctgttc tgctttaaag
     2221 cgcggcagca ctccaggaat agccaacgtc agcatacaag gcacgatcaa cacgcccgtt
     2281 gagaattgct cactgaacca gtccgcaagc aaaggccaga aatccagact atcaatactg
     2341 accgaaccaa tcgccccgac aatagcgcat aataatgcgg caatcagaca gtaattaaat
     2401 agccgtaaag cacttactgg ctcatactta tttttcccca gacgcttatc ccgcacgacc
     2461 agtaaggcga cggtaacaat aaacatcata ttggagaaat tgataaccag tgaaaccagt
     2521 ccccattcgg tggttatggc atcataaacc agcatcgcca cataactgat cgcatagtaa
     2581 tgcagtcgat taagccagac atagcgggca aacacccccg ccattacgcc attcagcggc
     2641 cagaaaagag aaagttcgtg aaccagccgt aactctgcac caataaaata gaacagcgtg
     2701 gttagcgcaa aaatagcgat gctattacgt agcagatgac cttcctggaa caaacggaaa
     2761 gttgaaatgg ggtgtacatg cattaattat ctgtccatac aattgcgggt ggcaattatc
     2821 tcaacgtgat aagtaaattc tcataaagga tagcatgttc gctaacagcg gcggcaaata
     2881 aaggtaccct gggaatgcta ttttatcgtt ttccgcaatt ctcatggctg attcatctat
     2941 acaaccagcc agtgatagat taccgccacg atataacgtt gttgttcttc gcttaacgaa
     3001 acaccctgcg gaatgttatg ccacttagcc agacaaccgc gacagcaggt cgcggtggcg
     3061 tgctgagcga taaacaccgg atgcccgcgc atgggggttt gcttaccgtc attagccggt
     3121 aatgccggag caagacgttt agcgacaaaa tccgccgcat gttgttcgat gactggcgcg
     3181 cctttctcca ggcaatactg acgctctttc atgcccagac gaaagcgaga gcgaaatttt
     3241 gaacgggata aacgcgcgaa gagtggatcg agagactgca ttaataaacc gaacgcccta
     3301 actgtttcgt caattgttca aggacggcga tacccgcaag cgagttacct gcatcatcca
     3361 gttccggact ccagactgcg atggccattt catgcggaac aatcgccaca ataccgccac
     3421 caacgccaga tttcgccggt agtcccaccc gccaggcaaa ctcccccgcg ttctggtaca
     3481 taccactggt cgccatcagc gcgttaattt gccgcgcctg cattggcgtc accacaggtt
     3541 catcaatatg aatagctttc ccctgattag ccagaaagac aaacgtccgg gccagctcta
     3601 cacagctcat tttcagagcg cagtaatgaa agtagttttg cagaacggtt gtcacgtcat
     3661 gatggaaatt gccaaacgac ttcatcagcc aggcgatagc cgcatttcgc gcggaatgtt
     3721 caaattcgga acgcgctacc accgtatcgt atgaaatatc agacacaccg cttaagccgc
     3781 gcacgacttc cagcatacgt tgccgtggtg cgcttagtcg cccttgcaac atatcgcaga
     3841 ccaccagcgc accggtatta atgaacggat tacgcggaat accctgttcc atttccagtt
     3901 gcactaaaga attgaacggt gatccagacg gatctttgcc gacgcgttgc cagatttcct
     3961 cttcggagta atgacgcatg gcgacaacga gactcagcac tttggaaata gactgaatgg
     4021 aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc gtcaacggta cagatagcaa
     4081 tccccaatcg ggaaccgtct actgtagcca gcgccggaat ataatccgcg actttaccct
     4141 gaccaatgag cggccgcact tgccgcaaga tgttttctaa aattgcatta tccatggcga
     4201 ctgccacttt ctactcctgg accgcaggtc tgaaaagacc tgcgagtata tcagagctga
     4261 atatgtcgcg tcagatccgg tctttccaca ctgtctggat attacagaat tcgtgtaagc
     4321 cgaaatggga aagctcacga ccaaagccac tctttttcac gccaccaaag gccactcgcg
     4381 cgtcgctggc acaataacca ttgataaaca ccccaccgca ttccagacgt gccgccatct
     4441 gtctggcctg tgtttcgtcg gtggtaaaaa tggtcgctga aaggccgaac tcactatcat
     4501 tagccagttc cagcgcatgt tctgcatctt tcgcaatggt gattgcagca acggggccaa
     4561 acatttcttc ccgaaacgcg gtcatttctg gggtaacatt cgccagaacc gttggcggat
     4621 agtagttacc tgctccagcc atcttttccc cgcccagtaa caaacgcgca ccctgcgcca
     4681 gggttttctc cacctgatga tgcagctcat cacgtaaatc aaaacgagcc attggtccga
     4741 gagcgttctc ttcatcacgg ggatcgccca ttttcaaggc tgccgcagct gacacaaaac
     4801 gttcggtaaa tgccgaagca attccctctt cgataataaa gcgttttgcc gctgcacaaa
     4861 cctgtccggt attctgataa cgtccggcta ccgccgcttt aaccgccagt tccagatcgg
     4921 catcgttaag cacaataaat ggatccgaac cgcccagttc cagtacgcat tttttcagtg
     4981 ccgctccagc acgaacactt ccggtcaccg tgacagcagc aatgcgcgag tctttaatca
     5041 tctgactgac accgtcgttg tcggcattca gccagccata tacgccttgc gggattcccg
     5101 catctttaaa cacttgggca atgagctgag cacagcccat cacattcggc gcatgtttaa
     5161 gtaagtagcc gttacctgca agaatgatgg gaacagcgcc acgcatcacc tgcctttgat
     5221 agttcttgca gctcaaatgc cctatatagt gatgttccga tgacttatga ctatatgggg
     5281 caaatatggt tacgccagta agcatcagca attacatatc tcttcctgat gattttcccg
     5341 ctcgtaatat tgcacctcaa gtaaaagaag ttttaaagga ttttattgat gcactcagta
     5401 ccataatatg tgatgaagag tggcgcacgt ctttaaacac caactcagcc acaaaaaaga
     5461 tatttaacaa ccttgataac ttatcatata ttcagaggac ttcttttcgg ggtaacgaca
     5521 cactatacaa tgaaaaggtt cagtttaaac tcacttatcc tgtcaagaat ggaagacaca
     5581 aagaaaatat tgaatttcag gtagtaataa atttaagccc tatttattta gataattttc
     5641 gccatgatgg agaaattaat attttttgcg ctcccaaccc aaagcctgtc actatggggc
     5701 gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt tatgaatgat tttattgaac
     5761 aatttccaat gatcaacctt ggcgccccca tcaaaagagc gcatacacca catattgaac
     5821 ccctgcctcc ggatcaccat accgctgcag attacttacg ccagtttgat ttgcttgtcc
     5881 tgaattttat ctctcgcggt aattttgtca tactcccccg attatggaat aactctgagg
     5941 ttcacagatg gttcgtcaat aaagatccta acttgatcac cgccattctc gacataacgg
     6001 acagcgaatt aaaagaggat ttattgcaaa gcctgatgga ttcattaggt tctaacaaac
     6061 atgtacaacc cgaagtctgc atctgcttct tatccctttt agcagaacaa gaatctcctc
     6121 attttcagga cttgttttta tttttcgcca atatgttatt gcactatcac caatttatga
     6181 atcccaatga aagtgatctg aatgacgtgt tgatgccagc atcattaagt gatgataaaa
     6241 ttatcaaaca tatggcgcgc aggcccctca aactgtttgt aaaaaatgaa acgccgccaa
     6301 aagttactca cgaagacctg gtgaaaaaca ggcctcgctc ccctgtcaga ccacctatac
     6361 ccgcaaccgc caaaacgcca gacctccctg aacgtcatta aaccgtgatg ttaccgactc
     6421 tctgactcgg aacagaatca gcgtcagaga aacggaaaac gcgatccaga tcacaaatgc
     6481 attgtattca catcattaac cgttttaaga tcatttcatc actttttcgc aactcacccg
     6541 ataatctgtt atgacaacaa acactgtttc ccgcaaagtg gcgtggctac gggtcgttac
     6601 gctggcagtc gccgccttca tcttcaacac caccgaattt gtccctgttg gcctgctctc
     6661 tgacattgcg caaagttttc acatgcaaac cgctcaggtc ggcatcatgt tgaccattta
     6721 cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta atgaccagtc aggttgaacg
     6781 gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc agccacgtac tgtcgttttt
     6841 atcgtggagc tttaccgttc tggtgatcag tcgcattggt gtggcttttg cacatgcgat
     6901 tttctggtcg attacggcgt ctctggcgat ccgtatggct ccggccggga agcgggcaca
     6961 ggccttgagt ttaattgcca ccggtacagc actggcgatg gtcttaggtt taccgctcgg
     7021 gcgcattgtg gggcagtatt tcggctggcg aatgaccttc ttcgcgattg gtattggggc
     7081 gcttgttacc cttttgtgcc tgattaagtt acttccctta ctgcctagtg agcattccgg
     7141 ttcattgaaa agcctcccgc tattgttccg ccgcccggca ttgatgagca tttatttgtt
     7201 aactgtggtg gttgtcaccg cccattacac ggcatacagc tatatcgagc cttttgtgca
     7261 aaacattgcg ggattcagcg ccaactttgc cacggcatta ctgttattac tcggtggtgc
     7321 gggcattatt ggcagcgtga ttttcggtaa actgggtaat caatatgcgt ctgcgttggt
     7381 aagtacggcg attgcgctgt tgctggtgtg tctggcactg ctgctacctg cggcgaacag
     7441 tgaaatacac ctcggggtgc tgagtatttt ctgggggatc gcgatgatga tcatcgggct
     7501 tggtatgcag gttaaagtgc tggcgctggc accagatgct accgacgtcg cgatggcgct
     7561 attctccggc atatttaata ttggaatcgg ggcgggtgcg ttggtaggta atcaggtgag
     7621 tttgcactgg tcaatgtcga tgattggtta tgtgggcgcg gtgcctgctt ttgctgcgtt
     7681 aatatggtca atcattatat ttcgccgctg gccagtgaca ctcgaagaac agacgcaata
     7741 gttgaaaggc ccattcgggc cttttttaat ggtacgtttt aatgatttcc aggatgccgt
     7801 taataataaa ctgcacaccc atacatacca gcaggaaccc catcaggcgg gagatcgctt
     7861 caatgccacc cttgcccacc agccgcataa ttgcgccgga gctgcgtagg cttccccaca
     7921 aaataaccgc caccaggaaa aagatcagcg gcggcgcaac catcagtacc caatcagcga
     7981 aggttgaact ctgacgcact gtggacgccg agctaataat catcgctatg gttcccggac
     8041 cggcggtact tggcattgcc agtggtacaa aggcaatatt ggcactgggt tcatcttcca
     8101 gctcttccga cttgcttttc gcctccggtg aatcaatcgc tttctgttgc ggaaaaagca
     8161 tccgaaaacc gataaacgcg acgattaagc cgcctgcaat tcgcagaccg ggaatcgaaa
     8221 tgccaaatgt atccatcacc agttgcccag cgtaatacgc caccatcatg atggcaaata
     8281 cgtacaccga ggccatcaac gactgacgat tacgttcggc actgttcatg ttgcctgcca
     8341 ggccaagaaa tagcgcgaca gttgttaatg ggttagctaa cggcagcaac accaccagcc
     8401 ccaggccaat tgctttaaac aaatctaaca ttggtggttg ttatcctgtg tatctgggtt
     8461 atcagcgaaa agtataaggg gtaaacaagg ataaagtgtc actctttagc taaccttgca
     8521 tcgcattgac caaaacttga accgatttag caaaacgtgg catcggtcaa ttcattcatt
     8581 tgacttatac ttgcctgggc aatattaccc cctacaacta attacttgcc agggcaacta
     8641 atgtgaaaag taccagcgat ctgttcaatg aaattattcc attgggtcgc ttaatccata
     8701 tggttaatca gaagaaagat cgtctgctta acgagtatct gtctccgctg gatattaccg
     8761 cggcacagtt taaggtgctc tgctctatcc gctgcgcggc gtgtattact ccggttgaac
     8821 tgaaaaaagt gttgtcggtc gacctgggag cactgacccg tatgctggat cgcctggtct
     8881 gtaaaggctg ggttgaaagg ttgccgaacc cgaatgataa gcgcggcgta ctggtaaaac
     8941 ttaccaccag cggcgcggaa atatgcgaac aatgccatca attagttggc caggacctgc
     9001 atcaagaatt aacaaaaaac ctgacggcgg acgaagttgc aacacttgag catttgctta
     9061 agaaagtcct gccgtaaaca aaaaagaggt atgacaatgt ccagacgcaa tactgacgct
     9121 attaccattc atagcatttt ggactggatc gaggacaacc tggaatcgcc actgtcactg
     9181 gagaaagtgt cagagcgttc gggttactcc aaatggcacc tgcaacggat gtttaaaaaa
     9241 gaaaccggtc attcattagg ccaatacatc cgcagccgta agatgacgga aatcgcgcaa
     9301 aagctgaagg aaagtaacga gccgatactc tatctggcag aacgatatgg cttcgagtcg
     9361 caacaaactc tgacccgaac cttcaaaaat tactttgatg ttccgccgca taaataccgg
     9421 atgaccaata tgcagggcga atcgcgcttt ttacatccat taaatcatta caacagctag
     9481 ttgataacgt gacaacgtca ctgaggcaat catgaaacca ctttcatccg caatagcagc
     9541 tgcgcttatt ctcttttccg cgcagggcgt tgcggaacaa accacgcagc cagttgttac
     9601 ttcttgtgcc aatgtcgtgg ttgttccccc atcgcaggaa caaccaccgt ttgatttaaa
     9661 tcacatgggt actggcagtg ataagtcgga tgcgctcggc gtgccctatt ataatcaaca
     9721 cgctatgtag tttgttctgg ccccgacatc tcggggctta ttaacttccc acctttaccg
     9781 ctttacgcca ccgcaagcca aatacattga tatacagccc ggtcataatg agcaccgcac
     9841 ctaaaaattg cagacccgtt aagcgttcat ccaacaatag tgccgcactt gccagtccga
     9901 ctacgggcac cagtaacgat aacggtgcaa cccgccaggt ttcatagcgt cccagtaacg
     9961 tcccccagat cccataacca acaattgtcg ccacaaacgc cagatacatc agcgacaaga
    10021 tggtggtcat atcaatagta accagactgt gaatcatgga tgcggaacca tcgaaaatca
    10081 gcgagacaac aaagaaggga atgattggga ttaaagcgct ccagattacc agcgacatca
    10141 ccgccggacg cgttgagtgc gacatgatct ttttattgaa gatgttgcca cacgcccaac
    10201 taaatgctgc agccagggtc aacataaagc cgagcatcgc cacatgctga ccgttcagac
    10261 tatcttcgat taacaccagt acgccaaaaa tcgctaaggc gatccccgcc aattgtttgc
    10321 catgcagtcg ctccccgaaa gtaaacgcgc caagcacgat agtaaaaaac gcctgtgcct
    10381 gtaacaccag cgaagccagt ccagcaggca taccaaagtt aatggcgcag aacagaaagg
    10441 caaactgcgc aaaactgatg gttaatccat accccaagag caaattcagt ggtactttcg
    10501 gtcgtgcaac aaaaaagatg gccggaaaag cgaccagcat aaagcgcaaa ccggccagca
    10561 tcagcggtgg catgttatga agccccactt tgatgaccac aaaatttagc ccccatacga
    10621 ccactaccag tagcgccaac accccatctt ttcgcgacat tctaccgcct ctgaatttca
    10681 tcttttgtaa gcaatcaact tagctgaatt tacttttctt taacagttga ttcgttagtc
    10741 gccggttacg acggcattaa tgcgcaaata agtcgctata cttcggattt ttgccatgcg
    10801 tccgatggtt aataacaacg acgaggcaag aagggcgctg gtagagcgtc gtagggataa
    10861 gttcatttgt ctgtccggca gtgcgtttcg ttatgtttta ttttagagat gtaaagaaat
    10921 agcatggcta cgctgccatt tatgaccatt tacttgagtc gccagtacag cctgagtgtc
    10981 gatctaatcg gttatgcgat gacaattgcg ctcactattg gcgtcgtttt tagcctcggt
    11041 tttggtatcc tggcggataa gttcgacaag aaacgctata tgttactggc aattaccgcc
    11101 ttcgccagcg gttttattgc cattccttta gtgaataacg tgacgctggt tgtgctcttt
    11161 tttgccctca ttaactgcgc ctattctgtt tttgctaccg tgctgaaagc ctggtttgcc
    11221 gacaatcttt cgtccaccaa caaaacgaaa atcttctcaa tcaactacac catgctaaac
    11281 attggctgga ccatcggtcc gccgctcggc acgctgttgg tgatgcagag catcaatctg
    11341 cccttttggc tggcagctat ctgttccgcg tttcccatgc ttttcattca aatatgggta
    11401 aagcgcagcg agaaaatcat cgccacggaa acaggcagtg tctggtcgcc gaaagtttta
    11461 ttacaagata aagcactgtt gtggtttacc tgctctggtt ttctggcttc ttttgtaagc
    11521 ggcgcatttg cttcatgcat ttcacaatat gtgatggtga ttgctgatgg ggattttgcc
    11581 gaaaagg
//
LOCUS       DAJVPP010000115        11346 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVPP010000115 DAJVPP010000000
VERSION     DAJVPP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.115"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(272..347)
                     /locus_tag="OQM34_003341"
     tRNA            complement(272..347)
                     /locus_tag="OQM34_003341"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(312..314),aa:Ile,seq:cat)
     gene            906..3158
                     /locus_tag="OQM34_003342"
     CDS             906..3158
                     /locus_tag="OQM34_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453667.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3494..4471
                     /gene="csiD"
                     /locus_tag="OQM34_003343"
     CDS             3494..4471
                     /gene="csiD"
                     /locus_tag="OQM34_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS1453668.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4491..5759
                     /gene="lhgO"
                     /locus_tag="OQM34_003344"
     CDS             4491..5759
                     /gene="lhgO"
                     /locus_tag="OQM34_003344"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS1453669.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5782..7230
                     /gene="gabD"
                     /locus_tag="OQM34_003345"
                     /pseudo
     CDS             5782..7230
                     /gene="gabD"
                     /locus_tag="OQM34_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7238..8524
                     /gene="gabT"
                     /locus_tag="OQM34_003346"
     CDS             7238..8524
                     /gene="gabT"
                     /locus_tag="OQM34_003346"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCS1453670.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8762..10162
                     /gene="gabP"
                     /locus_tag="OQM34_003347"
     CDS             8762..10162
                     /gene="gabP"
                     /locus_tag="OQM34_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS1453671.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10183..10845
                     /gene="csiR"
                     /locus_tag="OQM34_003348"
     CDS             10183..10845
                     /gene="csiR"
                     /locus_tag="OQM34_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS1453672.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10846..11295)
                     /gene="kbp"
                     /locus_tag="OQM34_003349"
     CDS             complement(10846..11295)
                     /gene="kbp"
                     /locus_tag="OQM34_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1453673.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
BASE COUNT         2907 a         2787 c         2833 g         2819 t
ORIGIN      
        1 tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcaatttgc
       61 ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga tgaataaata
      121 cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca cccaccgcac
      181 ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac cccttgtacc
      241 cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact tgaaccagcg
      301 accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta agtatgcaat
      361 aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat aacagatttt
      421 tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg acaccgcctc
      481 cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc actatctata
      541 gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat aactttatta
      601 cattagctga agagttttcg catcattatg atatctgtta cttttcactc cataaaaata
      661 aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt agtttcgtat
      721 gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac agatttaaac
      781 attatccttt tccatataga tattggataa aaataggaca tctgtttgca attactttca
      841 caacaattaa acatttttat gttttcgtat acatcatatt actctaccat tagaggaacg
      901 ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac cccacattgt
      961 tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta gaatatctaa
     1021 actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta aaagacagag
     1081 aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa aacgattata
     1141 aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca cagagtagca
     1201 ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga gatacaatgg
     1261 ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat ttcactcaca
     1321 ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt aacattcctg
     1381 actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc ggacagcaag
     1441 aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag cgtaaatctc
     1501 ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct ggcgaagcga
     1561 ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct gagtttgcac
     1621 gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc gacggaccag
     1681 aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa gatctttgta
     1741 ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa gatgctaaaa
     1801 aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc ggggggagtg
     1861 gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt aaagatatag
     1921 aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt aaagatccag
     1981 taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca aaagacttcg
     2041 aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca ttaaaagaaa
     2101 agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt atgttgcaaa
     2161 atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat gatgcagcaa
     2221 aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa aattataatg
     2281 agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc gttatgaatt
     2341 acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg ctgctttatg
     2401 aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata aaagcctttt
     2461 cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc tcaagcatcc
     2521 catcagaaag acggatattg attaacatta accacgattt tcctaacaat ggcaatcttt
     2581 tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca tatatggccg
     2641 ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa tcaacgactg
     2701 aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtggctt aataaaggtt
     2761 gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat aaagcaggaa
     2821 tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt agtcgtgggg
     2881 attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat aaaggaaacg
     2941 aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa aacatgggga
     3001 gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct tgtaaagtat
     3061 ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct cttcttttag
     3121 ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata tctgccccga
     3181 taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg aagccattta
     3241 ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa cctgttgctt
     3301 tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt tactaatttg
     3361 ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt tcagaaatgt
     3421 agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac atctcataag
     3481 aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg attcaggcca
     3541 ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc tggaactcac
     3601 cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc ccgtgcaggc
     3661 gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg atctgtgtgc
     3721 gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag gtgcgctgtt
     3781 gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga agctggcaac
     3841 ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc agtattacgc
     3901 gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc cgcaccgcgt
     3961 gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg tgctgatgat
     4021 gaaaatcgat gagcaaaaca tgcagggtgg aaattcgttg ctgctgcatc tcgatgactg
     4081 ggaacatctg gaccactatt tccgccatcc actggcgcgt cgcccgatgc gctttgccgc
     4141 gccgccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg ttgatcaaca
     4201 gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccaaaagatt ttgaagaagg
     4261 cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc aaaggcattc tttctgtacc
     4321 cgttcccgtt ggcaaattcc tgttgataaa caacctgttc tggctgcacg gtcgcgaccg
     4381 ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct atttcgctta
     4441 cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg atgtatgatt
     4501 ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg attgatgttt
     4561 atccggatgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt caccagacgg
     4621 gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc ctgaaggcgc
     4681 agttttgcct ggcggggaac cgtgccacca aagccttttg cgatcaaaac ggcattcgct
     4741 atgacaactg cggcaagatg ctggtcgcca cgtctcagct cgaaatggaa cggatgcgcg
     4801 ccttatggga acgcacagcg gcgaacggta tcgagcgcga gtggataaac gccgtggaac
     4861 tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat ttttgtgccg tccagcggca
     4921 ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat cttccaggcc agaggcggcg
     4981 agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc gtggtgatac
     5041 gcacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc gggctgatgg
     5101 ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc ccgttccgtg
     5161 gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg atttacccca
     5221 ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc gacggcagcg
     5281 tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc aagcgcgact
     5341 tctcatttag tgacacgctg gagattttga gctcgtcggg gattcgccgg gtgctgcaaa
     5401 accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc ggctatctgc
     5461 ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc tggcccgccg
     5521 gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat tgacgatttt ctgtttgtca
     5581 ccaccccacg cacgatccac acctgcaatg cgccctcccc ggcagcgaca tcagcaattc
     5641 ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag agtaaccccg
     5701 gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt aatcaataac
     5761 ctttgaaaac aggacgtaac aatgaaactt aacgacagta acttattccg ccagcaggcg
     5821 ttgattaacg gggaatggct ggacgccaac aatggtgaag tcatcgacgt caccaatccg
     5881 gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg cagatgaaac ccgcgccgct
     5941 atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc tcaccgccaa agaacgcgcc
     6001 accattctgc gcaactggtt caatttgatg atgcagcatc aggacgattt agcgcgcctg
     6061 atgaccctcg aacagggtaa accgctggct gaagcgaaag gcgaaatcag ctacgccgcc
     6121 tccttcattg agtggtttgc tgaagaaggc aaacgtattt atggcgacac cattcctggt
     6181 catcaggccg ataagcgcct gattgttatc aagcagccga ttggcgtcac cgccgccatc
     6241 acgccgtgga actttccggc ggcgatgatt acccgtaaag ccggtccggc gctggcggca
     6301 ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct ggcgctggcg
     6361 gaactggcga ttcgcgcggg cattccggct ggcgtgttta acgtggtcac cggttcggca
     6421 ggcgcggtcg gtaatgaact gaccagcaac ccgctggtac gcaaactgtc gtttaccggt
     6481 tagaccgaga ttggccgcca gttaatggaa cagtgcgcga aagacatcaa aaaagtgtcg
     6541 ctggagctgg gcggcaacgc gcagtttatc gtctttgacg atgccgatct cgataaagcc
     6601 gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt ctgcgccaac
     6661 cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca gcaggcggtg
     6721 agcaaactgc acatcggcga cgggctggag aaaggcgtca ccatcgggcc gctgatcgat
     6781 gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa aggtgcgcgt
     6841 gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact tcttccagcc aaccattctg
     6901 gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc cctcgcccca
     6961 ctgttccgtt ttaaagatga agccgatgtg attgcacaag ccaatgacac cgagtttggt
     7021 cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtcgg cgaggcgctg
     7081 gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc cccattcggc
     7141 ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga agattactta
     7201 gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaaggaaca gcaataaaga
     7261 gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc acccgatttt
     7321 cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt atcttgattt
     7381 cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg tggccgcggt
     7441 ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt acgagccgta
     7501 tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca agaaaacgct
     7561 gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc gcgccgccac
     7621 caaacgtagc ggcaccatcg cttttagcgg cgagtatcac gggcgcacgc attacaccct
     7681 ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc cgggtcatgt
     7741 ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg ctatcgccag
     7801 catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca tcatgattga
     7861 gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca gcctttatgc agcgtttacg
     7921 cgctctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga gcggcgcggg
     7981 gcgtaccggc acgctgtttg cgatggagca gatgggcgtg gcgccggatc ttaccacctt
     8041 tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc accgggcgcg cggaagtgat
     8101 ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc ggtaacccga ttgcctgcgt
     8161 ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat ctgctgcaaa aagccaacga
     8221 tctggggcag aagctaaaag acggattgtt ggcgatagcc gaaaaacacc cggagatcgg
     8281 cgacgtacgc gggctgggat cgatgattgc catcgagctg tttgaagatg gcgatcacag
     8341 caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata agggcctgat
     8401 tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccac tcaccattga
     8461 agacgctcag atccgtcagg gtctggagat tatcggtcag tgttttgatg aggcgaagca
     8521 gtagcaccgc ccccatgcag gaggcgacgc tgcgcgtctt gtccggccta cgagaatcca
     8581 agtcggataa ggcgtttacg ccgcatccga caatctgtac gtgaacagga ataaatctat
     8641 gtcggccgga taaggcggag ccgctctccg gcaaaaagaa tcaataacaa ttaaacgcgt
     8701 gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga gaggattcag
     8761 gatggggcaa tcatcgcaac cacacgagtt aggcggcggg ctgaaatcac gccacgtcac
     8821 catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca gcgtcgccat
     8881 cgccgaagcg gacccggcgg tattactggc ctatctgttc gccggattac tggtggttat
     8941 gattatgcgg atgctggcgg aaatggcagt tgccacgccc gataccggtt cgttttccac
     9001 ctatgccgat aaagccattg gccgctgggc gggctatacc atcggctggt tgtactggtg
     9061 gttttgggta ctggttatcc cactggaagc caacatcgcc gccatgatcc tgcactcgtg
     9121 ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct taactggcag
     9181 taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc tgtgcaaagt
     9241 catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt tttaccctta
     9301 tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc ccaacggttt
     9361 cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg cagaaattgt
     9421 caccattgcc gccgcggaat ccgacatgcc ggaaaaacat attgtccgcg ccaccaactc
     9481 ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtcg tggcattaat
     9541 accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc tggaattgct
     9601 caatattccc catgcgaaat taatcatgga ctgtgtgata ttactttccg taaccagttg
     9661 cctgaactcg gccctgtata ccgcctcaag gatgctctac tccttaagcc gtcgcggtga
     9721 tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtacgtgg cggtgttact
     9781 ctccaccggc gcagcattct taacggtggt ggtgaactat tacgcgcctg cgaaggtatt
     9841 taaatttctg atcgacagct ccggcgctat cgccctgctg gtttatttag tcatcgccgt
     9901 ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc gcttgcgaat
     9961 gtggcttgat ccctggctca cctggctggt catcggcttt attacctttg tgttggtagt
    10021 gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat tagcgatagg
    10081 gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt ggcaaaaaac
    10141 acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat tacgtctctg
    10201 gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc ggatgaaaaa
    10261 ttacggatga gtttactgac atcgcgttat gcacttggcg ttgggccgtt gcaggaagca
    10321 ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg gtatcgggtg
    10381 gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa tatggaagcg
    10441 atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga cgttctcgca
    10501 aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat gcttgatgag
    10561 tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg ttctcactat
    10621 ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt tatctggctg
    10681 cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca ccagaccctg
    10741 actgcggcgg tactgccgcg agatacagcg cgcgccagtg agttaatgcg ccagcattta
    10801 ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact cttccggaat
    10861 acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt tattcgcttc
    10921 gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg cactcagagt
    10981 gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg ttttcacctg
    11041 atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt tctccttcgc
    11101 ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt gaatgttcac
    11161 tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct tcgcctgatc
    11221 gtctttatcg tgctgacctg taaccgcatc ccagagtttt tctccggcat ctttaacaaa
    11281 attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag tgtagcagag
    11341 gaacgc
//
LOCUS       DAJVPP010000116        11320 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVPP010000116 DAJVPP010000000
VERSION     DAJVPP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.116"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..463)
                     /gene="cheZ"
                     /locus_tag="OQM34_003350"
     CDS             complement(<1..463)
                     /gene="cheZ"
                     /locus_tag="OQM34_003350"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS1453674.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMD"
     gene            complement(474..863)
                     /gene="cheY"
                     /locus_tag="OQM34_003351"
     CDS             complement(474..863)
                     /gene="cheY"
                     /locus_tag="OQM34_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS1453675.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(878..1927)
                     /gene="cheB"
                     /locus_tag="OQM34_003352"
     CDS             complement(878..1927)
                     /gene="cheB"
                     /locus_tag="OQM34_003352"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCS1453676.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(1930..2790)
                     /gene="cheR"
                     /locus_tag="OQM34_003353"
     CDS             complement(1930..2790)
                     /gene="cheR"
                     /locus_tag="OQM34_003353"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS1453677.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTVFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(2809..4410)
                     /gene="tap"
                     /locus_tag="OQM34_003354"
     CDS             complement(2809..4410)
                     /gene="tap"
                     /locus_tag="OQM34_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCS1453678.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAVLAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4456..6117)
                     /gene="tar"
                     /locus_tag="OQM34_003355"
     CDS             complement(4456..6117)
                     /gene="tar"
                     /locus_tag="OQM34_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCS1453679.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVVWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6262..6765)
                     /gene="cheW"
                     /locus_tag="OQM34_003356"
     CDS             complement(6262..6765)
                     /gene="cheW"
                     /locus_tag="OQM34_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCS1453680.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(6786..8750)
                     /gene="cheA"
                     /locus_tag="OQM34_003357"
                     /pseudo
     CDS             complement(6786..8750)
                     /gene="cheA"
                     /locus_tag="OQM34_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
     gene            complement(8755..9681)
                     /gene="motB"
                     /locus_tag="OQM34_003358"
     CDS             complement(8755..9681)
                     /gene="motB"
                     /locus_tag="OQM34_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCS1453681.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(9678..10565)
                     /gene="motA"
                     /locus_tag="OQM34_003359"
     CDS             complement(9678..10565)
                     /gene="motA"
                     /locus_tag="OQM34_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS1453682.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(10692..11270)
                     /gene="flhC"
                     /locus_tag="OQM34_003360"
     CDS             complement(10692..11270)
                     /gene="flhC"
                     /locus_tag="OQM34_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS1453683.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
BASE COUNT         2560 a         3189 c         2771 g         2800 t
ORIGIN      
        1 catccatcat ccgcttaata acctgcccgg tgagatcctg aaaatcctgc gccatcatga
       61 tttccagcag ttgcgcgtta gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc
      121 gtgtatctgt taccagttca cgggcgtcgg caaggtcaat cggatcggca aaccagtcat
      181 cccaacgttg ggttaacgct tttgctgatt tctccatttg atcctgatgc ggttgtgacg
      241 cctcaacact gttcagcgcc cgctccgcag cctgggcggt catctgcaca acatagtaca
      301 aacgatcgcg cgcatcgggg atggcttccg ccgcttcggc aatggcctga tccagcccca
      361 gttcccgcaa actgtcgcgc agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc
      421 cagctgaatg ctcgtcagca ggtttgattg atggttgcat catagtcgca tcctcacatg
      481 cccagtttct caaagatttt gttgagtttt tcctccagcg tcgcggcggt aaatggcttc
      541 accacatagc cactggcccc cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct
      601 gcagtcacca ttaacactgg caatgccgac atcgcgccat ccgcacgaat tgttttcagc
      661 aattccaggc catccatatt gggcatgttc cagtcggaga taacaaatcc ataaccgcct
      721 gcctgcaact tattgagagc gtcgacacca tcttccgctt cctcaacatt attgaatccc
      781 agctctttca gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa
      841 aatttaagtt ctttatccgc catttcacac tcctgattta aatacgtatc gcctgtccgg
      901 cactaattgt tgccaacatt tgctggctta cctggctaag atcgaccact tcgcagacac
      961 cacccatatt gatggcctcg cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg
     1021 caagggtcca tgcccccgcc tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca
     1081 taccggtcag gatcacccca accgcattac gccccgcctg tttggcgaca gaatggaaca
     1141 acacatctac cgaaggccga tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat
     1201 aatttgcgcc actacgcgcc agttccatat gccgatcgcc cggcgcaata taggcatgcc
     1261 ccggcaagac acgttctccg tcttcggctt ctttaacccc gatctggcaa agcttattaa
     1321 gtctgtcggc aaaagagcgg gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg
     1381 ggctggaaag cggcaacggt tgcagtacgt gacgaattgc ctcagttcca cccgttgaag
     1441 caccaatcgc aatcagtttt tcagaactca acaacggccc tgccttcagc gttgtcggtg
     1501 ctgacaatgg cttatgtgct gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa
     1561 tcatttcgct atacgccagc attccttcgc gaatacccag ttgcggtttg gtgacaaaat
     1621 ctattgcccc cagctccagc gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag
     1681 aaaccatcac aacgggcatt ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt
     1741 ccatccgcgg catttcaaca tccagcgtca gcacatcggg attgaatttc ttaatcaagt
     1801 cacgcgcaac cagcggatca ggcgcggtcg ccaccatttc catgtcgcta tggctgttga
     1861 tgatttctgt catgatctgg cgcatcagtg ccgaatcatc gacagataac accctgattt
     1921 tgctcatcgt taatccttac ttagcgcata caccgtctga ccacgcagcg tgaagcggcg
     1981 ctcgaggtgg ctaaagtttt cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag
     2041 cggaacaaag cggcgcaaaa tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt
     2101 acgacagaag atcgcatcaa acggccccgg cacggtgtac tgtttcgcca gtaaattcag
     2161 cggggcaaaa tcaacatagt tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg
     2221 tcccgtccct cgcatgaaat accgttgcag ttgctgcggc gtcaggtttt tcaactcttc
     2281 atggcgatag ataccgcttc tggctttttc cagcacttcg gtgtcgatat cactggcaaa
     2341 cactttccag cgtccgggca cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta
     2401 cggctcttcg ccggtcgaag ccgccgcgct ccatacgcga tcctcgccag aacggcgacg
     2461 tgcgtgatcc gcgagcagag ggaaatgatg agcctcacgg aaaaataccg tcagattcgt
     2521 ggtcagcgaa ttgataaacg cctgccactc accgctgtgc tgattagatt ccagcaagtt
     2581 cagataatga ccgaaatccg tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta
     2641 aaccatgtcg cgtttatggt cagccagaac gatcccggct cgttgataga tcaattgact
     2701 tatccgccga aaatgcgcgt cggaaagcgc caggcgctcg gtcatctgta acaataaaga
     2761 cgtttgccca cagggcagag atgaagtcat agcgccttct caatcacttc aggataccac
     2821 tggcgcaatt tgtaactgcg ccgactcatg tcgtgctact tcatgttctt caagggtaaa
     2881 taccgccaca cgcgacgaaa gatggtcggc ctgattcgcc agttgttccg ttgccaccgc
     2941 cgcttcttct accagcgagg agttctgctg agtcacctga tccatctggc tgacagcctg
     3001 tgcaacctgc tcaatccccc gccgttgttc ttccgacgcc gaagcaattt ctcccataat
     3061 gtcgttcacg cgggtcaccg aactgacaat atcggtcatg gtcgcggcgg cgttattcac
     3121 cagtttcgaa ccctgctgga cacgattgac tgactcttcg atcagccctt tgatctcttt
     3181 tgctgcctgc gcgctacggc tggcaagatt gcgcacttca cctgccacta ccgcaaaacc
     3241 acgcccctgc tctccggcgc gagccgcttc cactgccgca ttcagggcca gaatattggt
     3301 ctggaaagca attccgtcga taacgctgat aatgtcgcca attttttgcg agctggtggc
     3361 gatctcctgc atggtgtgag tcatggtact gacctgaaca ccgcccgcct gcgccgttgt
     3421 cgcggcattt tttgccagtt ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt
     3481 ggcggttaat tgctccatac tggcggccgt ttgtgccagc gatgccgcct gctgttcggt
     3541 acgacttgag agatcgttat tgcctgcgac aatctccgcg atcccaatgt gcatctcatg
     3601 gcttccctta cgcacatcac tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct
     3661 ggcaaaaatg gcggtgatct cattacgacc atataccgca atcggacgcg ccagattacc
     3721 ggcagcaatg ctgtcaaaat ggctaccgat aatggccagt ggttgaacaa tcatcttgcg
     3781 cgtccaccac agcgcactgc tgatgtagat cgccgcaaca ataatcatgc tgataaacac
     3841 cagcgccgaa atctgatagt tacgctggct ttgcgcactg gcggcttcca gcacatggtt
     3901 gatctccagc tgccaggcct caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg
     3961 cgcagtgagg aaatccgaaa gttgattact ttcaagccag gtcgcctgat gttcgagatc
     4021 gttgtgccag cgggcaaaac ttttctccgt ctctttttgc aatgccctga cgtgctcgtt
     4081 gcccgcagtc atcgccataa aacttttaaa cagcgtggtg gattgcgtca gactggcgcg
     4141 cgccgtcgtc atcaacgttt taatgtcatc cgccggatag ctaagcgctg tcagagtgcc
     4201 tgctttgttc agcgcggtac tggcctgtaa cattaccgcc cgagtttgcg ctaataccgc
     4261 acgttgctga ttgctctgct cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca
     4321 aaaagacatg ccgttactgc caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag
     4381 cgtggtcgaa attcgaatac gattaaacat caacgctccc catcaggcgg caatgaccgc
     4441 gttagtaaaa actcgtcaaa atgtttccca gtttggatct tgttcagtaa ttcgcagtcg
     4501 tggctgtgcc ggtggttgct cactggcagg acgggatggt gtttgcggtt tattggtgag
     4561 tgggctggct gccagacgga acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc
     4621 cagcgcagct gcggcggcag ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg
     4681 atccatttcc gaaaccgcca atgcgacttg atcgatgcca cggctctgtt catccgatgc
     4741 cgatgcaatc tcgcccataa tgtcagtcac gcgagtgaca gcattgacga tattgttcat
     4801 tgtttccccg gcgctttcga ccagcaccga accggtatca atgcgcgaga cggagtcttc
     4861 aatgagggct ttgatctctt ttgccgcctg ggcgctgcga ctggcaagat tacgcacttc
     4921 acccgccacc acggcaaaac cacggccctg ttcacccgca cgcgcggctt caaccgcggc
     4981 attcagcgcg aggatattag tctggaaggc aataccgtcg ataacgctga taatgtcggc
     5041 aattttcttc gaactatcgg cgatctcatg catcgttttc actacgccat ccaccacttt
     5101 gccgccgtgc tgggcggtgt cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt
     5161 atcggcgttt tgcttcactg tcgcggtgag ctgctccatg ctggcggcag tttcttccag
     5221 cgcggatgcc tgctgttcag tacgggagga aagatcggtg ttgcccgccg caatttcacg
     5281 ggtaccggca tagatggcat ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga
     5341 gcgttgcata tgtgaaacgc tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt
     5401 cagggtattc gccaggttac caccggcgat ttcgcaaatg tgagcaataa tttttgccag
     5461 cggagtaagc aacatacggc gaatgccgta ccacaccacc agcagaatca ataccaccac
     5521 cagcgcgata accgccagtt gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac
     5581 gatatcgcga tacagttttt cactgctgag ggcgtactga gcaaacgctt cgcccattgc
     5641 attttgcatt ccctgggttg gctgagcgaa ataagctcca gtattgccat aatcaagata
     5701 atcaatcagt tcagttaacg ctgtgtaata gtttttatat ttttcatcaa aattacggct
     5761 ggtagcgacc atttcaggta acggtgccat gcttttgaat tttttataat gcgtcgcggc
     5821 ctgggccaat gttttcctgg cgctatcgag caattcaact ttggcgttac tttgttgatt
     5881 ggaggaatcc atcatcatcc gcaccgctga acgactcagg ttaatgcgcg tttgcagcat
     5941 taaatcccag gttgacgtca gctcgccctg ctgttcccgt aattgattgg aaaccacaaa
     6001 gctcttctgg ctatggtgaa gggaagaaaa aaacaaactg ccggaaataa gctgtaacag
     6061 tgcgaatacc cccagcacca ttaccaacag cgtgactacg cggatacggt taatcataag
     6121 gcaccttcct gaaaacaagt tgatctcgtt atcggcaaga aggggggaaa ctttattgct
     6181 gatgccaccc gccgcgaaat tgaaataaaa aacccgatgc gcagatcatc gggttcattt
     6241 caattgagga aatcgggaga attacgccac ttctgacgcc gcgctatcta acagcgccat
     6301 ctcttcgctg ttcagcagtt tttcgatgtt caccagaatc aacatacggt cgcccagtgc
     6361 gcccagtcca gtgagatatt ctgttgaaag cgtcacggca aactctggtg ccggacgaat
     6421 ttgctccgcc gtcaatgaaa gcacgtctga aacgccgtca accacgatgc cgaccacccg
     6481 ctgtccgaga ttcaggacga taactaccgt gttgtcgtta tagtccacat ccacctggct
     6541 gaacttaatt cgtaagtcaa caatcggcac aataacgccg cgcagattcg tgacgccttt
     6601 gataaacgct ggcgtgttcg caatccgtgt tacctgatcg tagccacgga tctcctgcac
     6661 tttcaggata tcaataccgt actcttcatc accaagggta aataccagaa attcctggcc
     6721 tgacggctcg ctggccagct ttgttacatt cgtcataccg gtcatattgt taccttttta
     6781 ctcattcagg cggcggtgtt cgccatacgt tgttcgcggt ttatcgcctg caaggcggag
     6841 acatcaacaa tcagtgccac gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg
     6901 actttgcgat agttactttc aaggttttta accacaacct ggtgttgacc aattaattga
     6961 tccaccagca aggcgtagcg gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg
     7021 gcttcggttt tcgcgcccgc gacgttgaac actttccaca gttcgacgat gggcagatat
     7081 tcaccccgca cttccagcac ccgctcgccg ccggccagtg gatggagatc ggcttcacgg
     7141 ggttgcagtg attccataac agcattcagc ggcagaatga aaacttcatc cgcaacgcgt
     7201 acggacatgc cgtcgaggat ggccagcgtc agcggcagta aaatgcggat cgtagtgcca
     7261 gtaccctgct tcgactggat ttcgacatga ccgcccatct cctggatatt acgtttaacg
     7321 acgtccatgc cgacgccgcg cccggagacg tcggtgacct gctctgccgt ggagaagcct
     7381 ggtgcaaata tcagcattgc aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct
     7441 tgcgaggccg cttttgccag aattcgctca cggtttagcc ccgccccatc gtcggtcact
     7501 tcaatgcaaa tgttgccgcc ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt
     7561 ttacctgcgg cgagccgttt ttctggcagt tcaataccgt gatcgaggct attgcgtacc
     7621 aggtgggtca gcgggtcgat aatgcgttct atcaggctct tgtcgagctc ggtggaactg
     7681 cccaccagcg tcagttctac ctgcttgccg agttttcccg ccagatcacg caccagccgg
     7741 ggatagcgac taaagacata ttccatcggc atcatgcgaa tcgacatcac tgattcctgc
     7801 aaatcacggg cgttacgttg taactgcccc atgctggtaa tcaaatcacc atgattaacc
     7861 gggtccagtt cgctggaacg ctgggcaagc atggactggg tgataaccag ctcgccgacg
     7921 aggttaatta attgatcaac cttttctacc gctacacgga tgctggtgga ttcactgctg
     7981 cgggtcgttt tttcccgctc cacgcggccg gttggcgctt gttcggctgc cagtttaagc
     8041 acaggtgggg tagctatttt tggcgaaact tctactgttt caaaggtaat ctgatcggct
     8101 tcaatcacaa aacagagtac cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt
     8161 gccgagagag aatccgcccc tttcaccacg tcatttaacg ttgtcagatg ccccagctct
     8221 tcttccagca ggtcgacttc gccggccttc aggcgcgaaa ggataattcg tcgcggcaac
     8281 tgactgcgac tttgctcatc ttgcggttca cttttggcaa ccacacttaa tcgggtcact
     8341 gcggatggcg tttcgccttt cgcttctaat gccagttgac gcaaggccta gcagatataa
     8401 tcgtagctgg cggcatccgg ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg
     8461 atgtccttcg tttccaaaaa cagattgata atgtcggtgt tgagttgcat ctcacctcgt
     8521 ctggcttcat cgagcaggtt ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca
     8581 aaagttcctg cccctccttt gatcgagtgg gcagcccgaa agatggcatt caattgttcg
     8641 gcatctggcg cttccggctg caaaaccagc agatgctgct ccatatcagc caacagttcg
     8701 tccgcttcat caaaaaatgt ctgataaaaa tcgcttatat cgatgctcac gctgtcacct
     8761 cggttcggct gatggcattg tgggaacact gacctgtggt gcaacctcag gtttttctag
     8821 ggcgcttact ggctcattct ggctttcggc gttttcatgc aaaatggcct gttcggcttg
     8881 tttgttcagt accaacaggc tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct
     8941 taagcgcatc gttgccgcca tgccgacgac acgtaacact ttgccgctat ccaaccctcc
     9001 gaccatcagt tcgcggcggg atgcattggc ccgatcggca gaaagctccc agttgctgta
     9061 acctttctca ccgctggcgt agggaaaatc atcggtatga cctgaaaggc taatacggtt
     9121 gggaataccg ttcagcacag gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac
     9181 atcggcactg ccggttctaa acatcgggcg attctggcta tcgatgatct gaatacgtag
     9241 accttcctgg accagatcga ttttgagatg gagacgtaac gcccgcagtt tcggatcgga
     9301 ctctatcaac tggtcgagat caccccgcaa tttccgcaat cgactttgct ccatgcgttt
     9361 tttcagctct tcgatgttcg gctgcttatt cacttccccc tggctttggg tgtaatcatc
     9421 accaccgccg ggaattgggc tttcactatt ggaaatgcga tcgccgcccg taaccgctgt
     9481 cgccagtgga gtccggaagt actccgcaat ctgaatcaac tcttttgggc tggagatgga
     9541 gatcagccac atcaccagaa aaaaggccat catcgcagtc ataaagtcgg cataagcaat
     9601 cttccacgat ccgtgtgctg ccccgtggct tttggctttg cgtcgtttga cgacaataat
     9661 cggatgcgct tgattcttca tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc
     9721 gcacgcacat gctcttccag ttcaataaac gacggacgtt cgctggaata gagcgttttg
     9781 cgaccaaact caacggcgat aggcggtgcg taaccgttca gattagaaag cagagtgact
     9841 ttgacgcact gcatcatttt gctggtttcg gcgcttttct gacgtaaaac actcgctagt
     9901 ggggaaataa atccgtaagc caataaaata ccgaggaaag tccccaccat cgcatgtgcg
     9961 ataagcgccc ccagctcggc ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc
    10021 attacagccg cgacaatacc aaacgccgga agtgagtccc cgaccagcgc cagactgttc
    10081 gccgggactt ctgcctcgct ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt
    10141 tcgaaggtgt tcatgtgacc gctgataatc aggcgcagat aatcgacgat aaaatcaagc
    10201 atgacgctat ccgcgaggat gcgtgggtag ctggcgaaga tctcgctctc acggggattt
    10261 tcaatatcac gttccagcga aaacatcccc atctgccgcg atttcgccat caaccgataa
    10321 agcagagcca gcagatccat atacattgct ttggtgtatt tggagcgacg aaacagcaac
    10381 ggcagcgctt tcagcgtgcc tttaatcgct ttgccattat tgccgacgat aaacgaccca
    10441 atccctgcac cggcaataat caccagttca gcgggttgat agagtgctcc aaggcttcca
    10501 ccggtcatca aataaccgcc gaaaactgta ccgagaacaa ccaggtaacc taataagata
    10561 agcacgacat catccttcca ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac
    10621 gcgcgattgg ggaaaaaaaa agcagcggta cgtcgttacc gctgctggaa tgttgcgcca
    10681 caccgtatca gttaaacagc ctgtactctc tgttcatcca gcagttgtgg gataatatcg
    10741 gcaggattct gggaaagttt acgtcttttt actgcccggg atggcggttg acataagctg
    10801 caggcaaagc tgccaacagg ctggtgagcg tgggtaataa aattgccgcc gcagcagttg
    10861 cagctggaaa gttgcagtaa tccactttca acaaaccgca ccaatgtcca ggcacgggtt
    10921 aatgccagca gtggtccttc ttctgcttgt gggcactgtt caaggtataa acggtaggct
    10981 ttgatcaccg catcgacgcc attacacaaa ccggttttca gtaaaaactg ccatgcatta
    11041 cagaacatcg aagcatgaac gttttgttcc caggtcataa accagtcggt tgagaatggc
    11101 agcatgcctt tcggcggtgg gcttccgcgc agttctttat aaagttttat caggcgtccg
    11161 cgacttaact gtgtttcgct ttccagcatc tgcaaacgag cgcccagggt gatcaattcc
    11221 attgccagct gaatatcccg cgcttcctga acaatgcttt tttcactcat gatcaggccc
    11281 ttttcttgcg cagcgcttct tcaggctgat taacatcatt
//
LOCUS       DAJVPP010000117        11298 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVPP010000117 DAJVPP010000000
VERSION     DAJVPP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.117"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            416..613
                     /locus_tag="OQM34_003361"
     CDS             416..613
                     /locus_tag="OQM34_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453684.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(563..1756)
                     /locus_tag="OQM34_003362"
     CDS             complement(563..1756)
                     /locus_tag="OQM34_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1453685.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1892..3616
                     /gene="iucA"
                     /locus_tag="OQM34_003363"
     CDS             1892..3616
                     /gene="iucA"
                     /locus_tag="OQM34_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1453686.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3617..4564
                     /gene="iucB"
                     /locus_tag="OQM34_003364"
     CDS             3617..4564
                     /gene="iucB"
                     /locus_tag="OQM34_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1453687.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4564..6306
                     /gene="iucC"
                     /locus_tag="OQM34_003365"
     CDS             4564..6306
                     /gene="iucC"
                     /locus_tag="OQM34_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1453688.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6303..7640
                     /gene="iucD"
                     /locus_tag="OQM34_003366"
     CDS             6303..7640
                     /gene="iucD"
                     /locus_tag="OQM34_003366"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1453689.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7646..9841
                     /gene="iutA"
                     /locus_tag="OQM34_003367"
     CDS             7646..9841
                     /gene="iutA"
                     /locus_tag="OQM34_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1453690.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10392..10535)
                     /locus_tag="OQM34_003368"
     CDS             complement(10392..10535)
                     /locus_tag="OQM34_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453691.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10785..>11298
                     /locus_tag="OQM34_003369"
     CDS             10785..>11298
                     /locus_tag="OQM34_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1453692.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETN"
BASE COUNT         2831 a         2833 c         2924 g         2710 t
ORIGIN      
        1 caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca tcagtgagtc
       61 aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat taaaaaccag
      121 taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg tcagtgaaaa
      181 agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc gccaatgccc
      241 tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc tgtttgaacg
      301 cgcagcgcag actcccggaa taaaagctga ctggcttgtc agaatgaaat ttaataacag
      361 agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa gaatcatgga
      421 gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc agcaagccag
      481 aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc tgatttactc
      541 cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata aatacagaca
      601 caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt gcgacacctg
      661 ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg tgactggtag
      721 cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt tgataagcgg
      781 gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac aaccatagct
      841 gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc aggagcaggg
      901 ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc gcagctactg
      961 cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc gtagcgaact
     1021 ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca gccagtaata
     1081 ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg cactccggtg
     1141 tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc actggggctg
     1201 ccatcagcag ccccagtggc gccagaggat gaatggccag cattgctgcc gcgcacagcg
     1261 gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg gccatgcgat
     1321 tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt gccgacacgg
     1381 tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct gttgttatgg
     1441 cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg ccatatcctc
     1501 ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt ccccaccacg
     1561 gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat acagacagac
     1621 tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc tgtccgatac
     1681 cgagaagccc ggcactgaac gcaagtggcc agcaggacag tggtttttgg ggcgtatctt
     1741 cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt atttgtaaaa
     1801 ttaatttatc tgacaataac atttattgtt gataatgaga atcattattg acataattgt
     1861 tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa aaccagccac
     1921 agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag actggaaact
     1981 gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat cgctccattt
     2041 cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg cacgtctggt
     2101 cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta ttattgataa
     2161 gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc agcgcgtgct
     2221 ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg ccgctctgcg
     2281 tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac acgctttcca
     2341 ccctgcgcct aagtctcatg aaccgtttaa ccggcgggag gctgaacgct acctgcctga
     2401 tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa tcgccggtga
     2461 aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga atgcgcctca
     2521 gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt ggcaggggga
     2581 atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga ttaaagactt
     2641 aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct actgtgccac
     2701 cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca tccgtactct
     2761 gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa ccgacggctg
     2821 gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg gctgggccgg
     2881 gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc gtgaaaatct
     2941 gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc aggccgcacc
     3001 tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc gcctcggtat
     3061 cactgtgcaa caggccgccc atgcatgggt tgatgcatac tgtcagcaag tgctaaagcc
     3121 gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc aaaatattct
     3181 tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc agggcagcgc
     3241 ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg cggaaaatat
     3301 tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta actccacttt
     3361 tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc tgatggctcg
     3421 tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct gtctcaacta
     3481 cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc tgaacgatca
     3541 taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta acccgctgca
     3601 ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg gactgcgctg
     3661 tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg cggtgttgca
     3721 ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga tatttatcgc
     3781 cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg agccacaggc
     3841 gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct tctggcagtt
     3901 accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt ttgatgcaga
     3961 gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt accgccgtta
     4021 cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt ctgatgcaga
     4081 acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc aaagtggctc
     4141 actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg cattcccgct
     4201 gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg cggcggaaga
     4261 ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc tgctggttgg
     4321 tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc tgacacatta
     4381 cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca ccgataacca
     4441 gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt tcgacttccc
     4501 acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg aggtcggtct
     4561 gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa aatgttgtct
     4621 gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca ctactgcatt
     4681 aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg ctggctgtgg
     4741 attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac gctgctgatg
     4801 cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat gcaggatttg
     4861 tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact gagtgccagt
     4921 gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca tcctaaattc
     4981 gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata tgcgccagag
     5041 tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat gatctggcgc
     5101 tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc gcaggagttt
     5161 acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct gccgctgccg
     5221 gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga ttttgccgaa
     5281 ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca gtcgctgcgt
     5341 accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct gaccatctac
     5401 aatacctcat gctaccgggg gatccctggc agatatatcg ctgccggacc actggcttca
     5461 cgctggctgc aacaggtttt tgcgaccgac gccaccctgg tgcaaagcgg cgcagtgatc
     5521 cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct tgcccgggct
     5581 ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg ccgctggctg
     5641 aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga aaacaatcag
     5701 ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg gcttacgcaa
     5761 ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg tgtcgcgctt
     5821 attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca gcgtgttctg
     5881 ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga aatggactct
     5941 ttgcctcagg aggtgcgtga tgtcacatcc cgtctgagtg cggactactt aatccatgat
     6001 ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat ggttcgtctt
     6061 ggcgtacctg aaaggcgatt ttatcaactg ctggcagcag tgttgagtga ttacatgaac
     6121 aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc acaaatcatt
     6181 cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg cagtcgcatg
     6241 ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca ggaatatgaa
     6301 tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat ctcagcattg
     6361 ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa catcctcatt
     6421 tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc tttctgaaag
     6481 atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat ctggtgaagc
     6541 acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt gaagagtttt
     6601 ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt cataccgttg
     6661 aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt cggggagaat
     6721 attttgccca caatatctgc cttggtacag gaaaacaacc ttatttacca ccctgtgtga
     6781 agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg ccggacctga
     6841 gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg ttccttaatg
     6901 cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg cgtaataatt
     6961 ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa tatatttcag
     7021 gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag aaaatgacat
     7081 cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac caccgttttg
     7141 aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta acaactctgg
     7201 aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag ggcagagaga
     7261 gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg ccacaaatac
     7321 ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa gtgcgtgatg
     7381 acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc aacgccagca
     7441 tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga tctgcacgta
     7501 ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc gcactgattc
     7561 agtggcgcag cggcagccgg aaaaaaccac agccggaggc tgcttcttta actcactata
     7621 cagcaaatat tcaggaataa cgatgatgcg caaaaagtat atgccccggg ctcttggtcc
     7681 gctgcttctt gtcgtgctgt caccagctgt cgcccagcaa aacgatgata atgagatcat
     7741 agtgtctgcc agccgcagca atcgaactgt agcggagatg gcgcaaacca cctgggttat
     7801 cgaaaatgcc gaactggagc agcagattca gggcggtaaa gagctgaaag acgcactggc
     7861 tcagttaatc cccggccttg atgtcagcag ccagagtcga accaactacg gtatgaacat
     7921 gcgtggccgc ccgctggttg tcctgattga cggtgtgcgc ctcaactctt cacgttccga
     7981 cagccgacaa ctggactctg tcgatccttt taatatcgac catattgaag tgatctccgg
     8041 cgcgacggcc ctgtacggtg gcgggagtac cggagggttg atcaacatcg tgaccaaaaa
     8101 aggtcagccg gaaaccatga tggagtttga ggctggcaca aaaagtggct ttaacagcag
     8161 taaagatcac gatgagcgca ttgccggtgc tgtctccggc ggaaatgacc atatctccgg
     8221 acgtctttcc gtggcatatc agaaatttgg cggctggttt gacggtaacg gcgatgccac
     8281 cctgcttgat aacacccaga ccggcctgca gcactccaat cggctggaca tcatgggaac
     8341 cggtacgctg aacatcgatg aatcccggca gcttcaactg ataacgcagt actataaaag
     8401 tcagggagac gacaattacg ggcttaatct cgggaaaggc ttttccgcca tcagcgggag
     8461 cagcacacca tacgtcagta aggggctgaa ttctgaccgc attcccggca ctgagcggca
     8521 tttgatcagc ctgcagtact ctgacagtga tttcctggga caggaactgg tcggtcaggt
     8581 ttactaccgc gatgagtcgt tgcggtacta cccgttcccg acggtaaatg cgaataaaca
     8641 ggcgacggct ttctcctcgt cacagcagga taccgaccag tacggcatga aactgactct
     8701 gaacagccaa ctgatggacg gctggcaaat cacctggggg ctggatgctg agcatgagcg
     8761 ctttacctcc aaccagatgt tcttcgatct ggctcaggca agtgcttccg gagggctgaa
     8821 caaccataag atttacacca ccgggcgcta tccgtcatat gacatcacca atctggcggc
     8881 cttcctgcaa tccagctatg acattaatga tatttttacc gttagcggtg gcgtacgcta
     8941 tcagtatact gagaacaggg tagatgattt catcgactac acgcagcaac agaagattgc
     9001 tgccgggaag gcgatatctg ccgacgccat tcctggtggt tcggtagatt acgataactt
     9061 tctgttcaat gctggtctgc tgatgcacat caccgaacgt cagcaggcat ggttcaattt
     9121 ttcccagggg gtggcattgc cggatccggg gaaatattat ggtcgcggca tctatggtgc
     9181 agcagtgaac ggccatcttc ccctgacaaa gagcgtgaac gtcagcgaca gtaagctgga
     9241 aggcgtgaaa gtcgattctt atgaactggg ctggcgcttt atcggtgaca acctgcggac
     9301 tcaaatcgcg gcatattact cgctttccaa taagagcgtg gaaaggaata aagatctgac
     9361 catcagtgtg aaggacgaca ggcgccgtat ttacggcgtg gaaggtgcgg tggactacct
     9421 gatcccggat actgactgga gtaccggtgt gaacttcaat gtgctgaaaa ccgagtcgaa
     9481 agtgaacggt caatggcaaa aatatgacgt gaaggaatca agtccatcga aagcgacagc
     9541 ttacattaac tgggcgccgg aaccgtggag tctgcgtgta cagagcacca cttctttcga
     9601 cgtaagcgat gcagagggta acgatattaa tggttacact accgtcgatt ttatcagtag
     9661 ttggcagctt ccggtgggaa cactcagctt cagcgttgag aacctcttcg accgtgacta
     9721 taccactgtc tggggacagc gtgcacctct gtactacagc ccgggttacg gccctgcttc
     9781 actgtacgac tacaaaggcc ggggccgaac ctttggtctg aactactcag tgctgttctg
     9841 acggggatgc ttttgcaaca agggtatgct gtaaacaaca tggtccttgg tggccatgtt
     9901 gtttacagcc agtataatat acagccttaa caagatattc cgtctccgga atgggctgtt
     9961 caccactgaa taacattatg tctgttttat ggatctttga ctgaaattta tcgcatatcc
    10021 tcatttcatt gtccgtctga aaactaacct tacaagtaag cgtcgtctca gcaccgtctg
    10081 gcagatcctg aaattcctga gaggatgatc tgcgcccaat atcctgagcc ggaactttgc
    10141 tgcaatgaag gccaacgaaa aatgggggag tatgttatag agtacttgct acagggtaaa
    10201 aagaggtacc tgtcgctggt tctccgttca gtttaaccgg gagattttct cctgcgcctg
    10261 tcataaagtc cggtaatgga gattgttaat acgatggtgc acgatgcatc gtcagtgccc
    10321 ggcccggagg atgccccgta gctacatacg gatcagggtt ggcaatatcg aatggcaggc
    10381 tataggcaaa gttaaagccc catggagtag cacaaaatat gccgcacaaa ggaaacggtc
    10441 tgaataacgc agtgatggag aacttcttcg gcacactgca aaatcgcatc gtggtcgatc
    10501 gctaattcgt cgtgaatcac tgaacgatgt tccatgcgaa cagatattcg atcatatcga
    10561 aatattaaac attacccctc cccacattgg gcaaaaaaaa acaaaaaacg atcaattaac
    10621 gatcaattag tattgattca cataatctct catcattaat gagaacattg tcacatattt
    10681 acaatataga actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta
    10741 attcttaata ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact
    10801 cccttaaata tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag
    10861 tttctggtaa aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta
    10921 cagtaaatgc agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg
    10981 cacaaaataa aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg
    11041 gagataaatt ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg
    11101 gcgcagctac cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc
    11161 ctcaggccgc agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa
    11221 attccaatga ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca
    11281 ccccggcaga gaccaacc
//
LOCUS       DAJVPP010000118        11225 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVPP010000118 DAJVPP010000000
VERSION     DAJVPP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.118"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1208)
                     /gene="cusC"
                     /locus_tag="OQM34_003370"
     CDS             complement(<1..1208)
                     /gene="cusC"
                     /locus_tag="OQM34_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCS1453693.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQ"
     gene            1365..2048
                     /gene="cusR"
                     /locus_tag="OQM34_003371"
     CDS             1365..2048
                     /gene="cusR"
                     /locus_tag="OQM34_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCS1453694.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2038..3480
                     /gene="cusS"
                     /locus_tag="OQM34_003372"
     CDS             2038..3480
                     /gene="cusS"
                     /locus_tag="OQM34_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCS1453695.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            3630..5867
                     /gene="nrfB"
                     /locus_tag="OQM34_003373"
     CDS             3630..5867
                     /gene="nrfB"
                     /locus_tag="OQM34_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCS1453696.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            5854..8822
                     /gene="nfrA"
                     /locus_tag="OQM34_003374"
                     /pseudo
     CDS             5854..8822
                     /gene="nfrA"
                     /locus_tag="OQM34_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            8823..9713
                     /locus_tag="OQM34_003375"
     CDS             8823..9713
                     /locus_tag="OQM34_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCS1453697.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            9896..10657
                     /gene="envY"
                     /locus_tag="OQM34_003376"
     CDS             9896..10657
                     /gene="envY"
                     /locus_tag="OQM34_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCS1453698.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(10703..10779)
                     /locus_tag="OQM34_003377"
     tRNA            complement(10703..10779)
                     /locus_tag="OQM34_003377"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10743..10745),aa:Arg,seq:tct)
     gene            11022..>11225
                     /locus_tag="OQM34_003378"
     CDS             11022..>11225
                     /locus_tag="OQM34_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCS1453699.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIILKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         2737 a         2928 c         2883 g         2677 t
ORIGIN      
        1 atttgcagcg acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt
       61 aatgccagcg catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc
      121 accgactgct gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta
      181 aaaatgggga tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat
      241 gacgacagat cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat
      301 gccgcacgcg cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata
      361 atatccgggc gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc
      421 aggctgccgc tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt
      481 tgcaatgcat tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta
      541 ctttctatca ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt
      601 tgtttttcga caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc
      661 aattgcgcat acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa
      721 atatgcaccg cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc
      781 atgttcttta agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac
      841 tcccgcgtcg tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctcgcca
      901 ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact
      961 ttcagcgtcg ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc
     1021 acctgattat caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta
     1081 accaggccgt tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa
     1141 tccggtgcca gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa
     1201 ggagacatag gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct
     1261 gattttccgg tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct
     1321 gatcccgcta ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg
     1381 tcgaagatga aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg
     1441 tggtcgattt ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg
     1501 acctgataat cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt
     1561 tgcgctccgc caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac
     1621 atcgcgtcaa ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg
     1681 ctgaactgct ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa
     1741 gtcagtttca ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc accctcagcg
     1801 gcacgcgcat cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc
     1861 agggcgaagt gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca
     1921 gcgacactaa cgccattgat gtggcggtga agcggctacg cggcaaaatc gacaacgact
     1981 ttgagccgaa gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg
     2041 gtcagtaagc catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc
     2101 ctggccacca tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg
     2161 cattttgccg agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta
     2221 ctaaatcacc ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt
     2281 ggttattcca acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc
     2341 cccggtgcgc ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg
     2401 ggtggcgagg tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg
     2461 catatggaac acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc
     2521 aaaccgattt atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat
     2581 gatttgatga ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc
     2641 gtgctgttgg cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag
     2701 aatatcacct cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg
     2761 gaacagctgg tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc
     2821 cagtccaatt tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt
     2881 acgcaaacgg aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc
     2941 tactctaatc tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc
     3001 gctcaggccg ataacaacca gctaatcccg gaaaagaaaa tgctcaacct ggcggatgaa
     3061 gtcggcaaag tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg
     3121 tttgttggcg acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc
     3181 aacctgcttt ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt
     3241 cagacggtcg atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc
     3301 gagcacttac cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa
     3361 ggtgaaggta gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc
     3421 acggttgcgg taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa
     3481 ttattcagca aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata
     3541 catattaatg gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa
     3601 cataaccagc gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct
     3661 ggctctacgg cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc
     3721 tggacgattt ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg
     3781 tttatcgccg ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt
     3841 tagcgattat ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg
     3901 cggcgaccac gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc
     3961 ccgatactca acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag
     4021 tctgcgcgcg tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca
     4081 tcacccagtt tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg
     4141 aagatgtgat ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc
     4201 tgattcagat cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt
     4261 acattgatga gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg
     4321 gacaagtgcc cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt
     4381 tagctgacgg taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg
     4441 gcttccgcct gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg
     4501 aagccaaaga acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct
     4561 gcgtgcgcga atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga
     4621 tcatcggcat tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga
     4681 actactttct ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga
     4741 tgctggtgat gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct
     4801 ggcatttcct ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact
     4861 ttggcttgat ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc
     4921 tcacgcaggg cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg
     4981 ctaactggcg cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg
     5041 ataaaacaac gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag
     5101 gtcaaattct gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta
     5161 atcgcgtcga aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg
     5221 agcagctggc acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct
     5281 ggcagatccc gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag
     5341 tactgccgct gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc
     5401 cggtttcgct ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc
     5461 tgcgaggaca aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc
     5521 cgcgggcaat gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg
     5581 aaatctggcg gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg
     5641 gtcatattaa tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc
     5701 cgctcggcaa gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc
     5761 tgacaattca acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa
     5821 acacagaaca ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc
     5881 atcggatggt ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat
     5941 atcggcacca gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc
     6001 cgtctcgata aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa
     6061 tttgaatata tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa
     6121 gcctatcgtc attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa
     6181 cgtcacccag gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa
     6241 agcgttacga ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc
     6301 ctgcgttgtc gcagtgaagt cgggcagaat gccctgcggc tggcacaatt acctgtcgcc
     6361 agagcgcaac tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc
     6421 gatctgctgc aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat
     6481 gaagcacgcc agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt
     6541 cttgccgggc agctggacga tcggatcctg gcactgcaat cacaggggat cttcaccgat
     6601 cctcagtcat atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc
     6661 cagcattatc tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg
     6721 ctctatctgt tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg
     6781 cagtttgccg ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa
     6841 ggtcagtacg acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag
     6901 gagcgttatg ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga
     6961 ttgctgtatc agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg
     7021 atgcagaacg agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag
     7081 ggcgatgcgc gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat
     7141 ccttacctag caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag
     7201 caacgtcagt ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc
     7261 ttgctgggcg atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt
     7321 tatcgggaca cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa
     7381 ccgagcgcct ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc
     7441 accgcactgg cggcctggca gaaaatcagt cttcacgaca tgagcaatga tgatctgctc
     7501 gccgccgcca acaccgctca ggcagcggga aatggcgcgg ctcgcgatcg ctggcttcaa
     7561 caggcagaac aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt
     7621 tatatcccag ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc
     7681 ccttctgcca actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg
     7741 gccgcggtga gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag
     7801 gcagcgcttg gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc
     7861 gaacaggcgc ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg
     7921 aaccagcgtc tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac
     7981 attgataatc aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc
     8041 gccgtttgca tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct
     8101 tgcgttccgg cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa
     8161 gctatcgtag ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg
     8221 aaggcgacct gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga
     8281 tgatgccggt gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc
     8341 agatcttttt cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg
     8401 ataccatgct gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc
     8461 acccgaacgg ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc
     8521 gccaggatat tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta
     8581 acggacagac tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca
     8641 ctcagggcgc gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc
     8701 actacgacgc ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg
     8761 cgattaatca acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt
     8821 aaatgcgtaa gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg
     8881 cgatgaaagg tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt
     8941 ggcaggggct gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga
     9001 cccgttacgg cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg
     9061 cagctgcgca acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt
     9121 ttatgcacca gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg
     9181 ccgatctcca gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt
     9241 acatcagcgc ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc
     9301 taacatggtt aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata
     9361 tcagtagttt tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag
     9421 ttaaagcaac cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg
     9481 ctgaacagcg tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa
     9541 gcggcattgt ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga
     9601 aaccggacgc agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc
     9661 tctatttctc tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct
     9721 ccaggtaagt cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga
     9781 ttaattgcta aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact
     9841 catgcaggaa gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca
     9901 attgagcagc agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt
     9961 caataaccat gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa
    10021 taacgtcatt gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat
    10081 catcaacgat tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg
    10141 gctggcaacc ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac
    10201 caaccacagc aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact
    10261 ttttaccgta ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat
    10321 cttacgcagc agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta
    10381 ctggaatctg cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa
    10441 attaaaaaac gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc
    10501 cgtacagatg ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta
    10561 tagcagcacg tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa
    10621 ttatctcgcc aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag
    10681 acgggcataa atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac
    10741 ttagaaggtc gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt
    10801 aatcgcgtga attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga
    10861 acaagtgaac gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca
    10921 caccgctgcg cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc
    10981 gccctaacgg aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga
    11041 tcattatgga tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca
    11101 gtgaattgca gattatcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa
    11161 cccgtcctgt tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct
    11221 tcctg
//
LOCUS       DAJVPP010000119        10841 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVPP010000119 DAJVPP010000000
VERSION     DAJVPP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.119"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..400
                     /locus_tag="OQM34_003379"
                     /pseudo
     CDS             1..400
                     /locus_tag="OQM34_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(524..1326)
                     /gene="glcC"
                     /locus_tag="OQM34_003380"
                     /pseudo
     CDS             complement(524..1326)
                     /gene="glcC"
                     /locus_tag="OQM34_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            1577..3076
                     /gene="glcD"
                     /locus_tag="OQM34_003381"
                     /pseudo
     CDS             1577..3076
                     /gene="glcD"
                     /locus_tag="OQM34_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3076..4128
                     /gene="glcE"
                     /locus_tag="OQM34_003382"
     CDS             3076..4128
                     /gene="glcE"
                     /locus_tag="OQM34_003382"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCS1453700.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPSTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4139..5358
                     /gene="glcF"
                     /locus_tag="OQM34_003383"
                     /pseudo
     CDS             4139..5358
                     /gene="glcF"
                     /locus_tag="OQM34_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            5363..5767
                     /locus_tag="OQM34_003384"
     CDS             5363..5767
                     /locus_tag="OQM34_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCS1453701.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            5789..7960
                     /gene="glcB"
                     /locus_tag="OQM34_003385"
     CDS             5789..7960
                     /gene="glcB"
                     /locus_tag="OQM34_003385"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCS1453702.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8315..9910
                     /locus_tag="OQM34_003386"
     CDS             8315..9910
                     /locus_tag="OQM34_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCS1453703.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2568 a         2658 c         2990 g         2625 t
ORIGIN      
        1 aatatctggt gccggacgat atgatctaca tcgctgagat tgattctgca ccctgcgcgt
       61 ttattgtggc ttgcctaaca tcaacgaggc gattgccgat ctgaacggat cgctctttcc
      121 cttcggctgg gcaaaattgc tgtggcgttt gaaagtcagc ggtgtgcgaa ctgcgcgagt
      181 accgctgatg ggcgtgcgcg acgagtatca gttcagccgc atcggcccgg tgattgcgct
      241 gttattgatt gaagccttac gcgatccgtt tgcccgccgg aagatcgatg cgctggagat
      301 gtcatggatc ctcgaaacca atacaggcat gaataacatg ctggaacgca ttggggcgga
      361 gccgtacaag cgttaccgat tgtatgaaaa gcaaatttaa taccagatcc tgtttagcgc
      421 aggggcggtc attcgggaga gggtatagat gaacagattt aacaccagat cctgttaagt
      481 gcaggggcgg tcccctcgcc ttggggatag ggtaaaactc tgctcacatt cccaccttgt
      541 tcatctccag cggcaccgcc gagcgaatca aatggtggcc ttccagctcg atatcgtgga
      601 gattcttttt cacggtccgt acatgatcgc gcgctgcgcg ctgcgcgctg cgcgctgagc
      661 gacgtgcggc agccgctgca acaccgcgtt gtagatccgc gcatgctggc gatcgatctg
      721 ctttttttgc tgtggtcgat ggtagagatt atttacgctg gcaaacactg aattaaacat
      781 cagatcggtc aatgattgca gcgtaaacac cagcacctga ttgtgagaag cctgacaaat
      841 ggcgagatgg aaagcgtgat ccaactgcgc atgttcgatc agcgaaatct ctttgtggtt
      901 ctcactggcg gcgagcattt tttcataaca gcgggttatc acaacaaaat cagcctgcgt
      961 tcccagcgtt cccagcgttc ccagcgttgc cgccagcctt gccgattcgc cctccagtaa
     1021 tgcgcgaacg tcgagcagat cgtacagcgt tcgcggctgc gtactgaaca gatggatcag
     1081 cgggctggtg tcctgctccc gattaagccg tgcgacatga gaatcgcgac cctgcgccgt
     1141 ttcaataatc ccgcgcccgc gcagcacggt cagcccttca cgcagtgcgg agcgtgagaa
     1201 gccgagcttt tcacacagtc gacgttccga gggaagcggc tgaccgacct tcagtacgcc
     1261 gtcgataatt aaccgttcga tactctctgc aaccacttcg caaatagggc gacgttcatc
     1321 tttcattccc ggacctcgtg cacaggtagg accaattttt ctgttcttaa ctcgcaaaac
     1381 acgcacatca cgcaagtgat tatgttacat caatttaaca ttaagttaac caagacaagg
     1441 tcacagagct ggaaaaaaaa tggtctgacc ggtagctaaa gagatagacg aaaacgaaaa
     1501 gcccgcttaa taactgttca cagaagcagc gcgcaaaaat cagctgccac acaacacaac
     1561 aaagcgaagc ctactcatga gcatcttgta cgaagagcgt cttgatggcg ctttacccga
     1621 tgtcgaccgc acatcggtac tgatggcact gcgtgagcat gtccctggac ttgagatcct
     1681 gcataccgat gaggagatca ttccttacga gtgtgacggg ttgagcgcgt atcgcacgcg
     1741 tccattactg gttgttctgc ctaagcaaat ggaacaggtg acagcgattc tggctgtctg
     1801 ccatcgcctg cgtgtaccgg tggtgacccg tggtgcaggc accgggcttt ctggtggcgc
     1861 gctgccgctg gaaaaaggtg tgttgttggt gatggcgcgc tttaaagaga gcctcgacat
     1921 taaccccgtt agtcgccgcg cgcgcgtgca gccgggcgta tgtaacctgg cgatctccta
     1981 ggccgttgcg ccgcataatc tctattacgc gccggatcct tcctcacaaa tcgcctgttc
     2041 aattggcggc aatgtggcgg aaaatgccgg tggcgtccac tgcctgaaat atggtctgac
     2101 cgtacataac ctgctgaaaa ttgaagtgca aacgctggac ggcgaggcgc tgacgctggg
     2161 atcggacgcg ctggattcac ctggttttga cctgctggcg ctgttcaccg gatcggaagg
     2221 tatgctcggc gtgaccaccg aagtgacggt aaaactgctg ccgaagccgc ccgtggcgcg
     2281 ggttctgtta gccagctttg actcggtaga aaaagccgga cttgcggttg gtgacatcat
     2341 cgccaatggc attatccccg gcgggctgga gatgatggat aacctgtcga tccgcgcggc
     2401 ggaagatttt attcatgccg gttatcccgt cgacgccgaa gcgattttgt tatgcgaact
     2461 ggacggcgtg gagtctgacg tacaggaaga ctgcgagcgg gttaactaca tcttgttgaa
     2521 agcgggcgtg actgacgtcc gtttggcaca ggacgaagca gagcgcgtac gtttctgggc
     2581 cggtcgcaaa aatgcgttcc cggcggtagg acgtatctcc ccggattact actgcatgga
     2641 tggcaccatc ccgcgtcgcg ccctgcctgg cgtactggaa ggcattgccc gtttatcgca
     2701 gcaatatgat ttacgtgttg ccaacgtctt tcatgccgga gatggcaaca tgcacccgtt
     2761 aatccttttc gatgccaacg aacctggtga atttgcccgc gcggaagagc tgggcgggaa
     2821 gatcctcgaa ctctgcgttg aagttggcgg cagcatcagt ggggaacatg gcatcgggcg
     2881 agaaaaaatc aatcaaatgt gcgcccagtt caacagcgat gaaatcacga ccttccatgc
     2941 ggtcaaggcg gcgtttgacc ccgatggttt gctgaaccct gggaaaaaca ttcccacgct
     3001 acaccgctgt gctgaatttg gtgccatgca tgtgcatcac ggtcatttac cttttcctga
     3061 actggagcgc ttttgatgct acgcgagtgt gattacagtc aggcgctgct ggagcaggtg
     3121 aatcaggcaa ttagcgataa aacgccgctg gtgattcagg gcagcaatag caaagccttt
     3181 ttaggtcgcc cagtcaccgg gcaaacgctg gatgtgcgtt gtcatcgcgg cattgttaat
     3241 tacgacccga ccgagctggt gataaccgct cgtgccggaa cgccgctggt ggcgattgaa
     3301 gcggcgctgg agagcgccgg gcaaatgctt ccctgcgagc cgccgcatta tggtgaagaa
     3361 gccacctggg gcgggatggt cgcctgcggg ctggcggggc cgcgtcgccc gtggagcggt
     3421 tcggtccgcg attttgtcct cggcacgcgc atcattaccg gcactggaaa acatctgcgt
     3481 tttggtggcg aagtgatgaa aaacgttgcc ggatacgatc tctcacggtt aatggccgga
     3541 agctatggtt gtcttggcgt gctcactgaa atctcaatga aagtgttacc gcgaccgcgt
     3601 gcctctctga gcctgcgtcg ggaaatcagc ctgcaagaag ccatgagtga aatcgccgag
     3661 tggcaactcc agccattacc cattagtggc ttatgttact tcgacaatgc gttgtggatc
     3721 cgccttgagg gcggcgaagg atcggtaaaa gcagcgcgtg aactgctggg tggcgaagag
     3781 gttgcgggtc agttctggca gcaattgcgt gaacaacaac tgccgttctt ctcgttacca
     3841 agtaccttat ggcgcatttc attacccagc gatgcgccga tgatggattt acccggcgag
     3901 caactgatcg actggggcgg ggcgttacgc tggctgaaat cgacagccga tgacaatcaa
     3961 atccatcgca tcgcccgcaa cgctggcggt catgcgaccc gctttagtgc cggagatggt
     4021 ggctttgccc cgctatcggc tcctttattc cgctatcacc agcagcttaa acagcagctc
     4081 gacccttgcg gcgtgtttaa ccccggtcgc atgtacgcgg aactttgagg agcaggctat
     4141 gcaaacccga ttaaccgaag agatgcggca gaacgcgcgc tggaagccga cagcatcctg
     4201 tgcgcctgtg ttcactgcgg attttgtacc gcaacctgcc caacctatca gcttctgggc
     4261 gatgaactgg acgggccgcg cgggcgcatc tatctgatta aacaggtgct ggaaggcaac
     4321 gaagtcacgc ttaaaacaca ggagcatctc gatcgctgcc tcacttgccg caattgtgaa
     4381 accacctgtc cttctggtgt gcgctatcac aatttgctgg atatcgggcg tgatattgtc
     4441 gagcagaaag tgaaacgccc actgccggag cgaatgctgc gcgaaggatt gcgccaggta
     4501 gtgccgcgtc cggcggtctt ccgtgcgctg acgcaggtag ggctggtgct gcgaccgttt
     4561 ttaccggaac aggtcagagc aaaactgcct gctgaaacgg tgaaagctaa accgcgtccg
     4621 ccgctgcgcc ataagcgtcg ggttttaatg ctggaaggct gcgcccagcc tacgctttcg
     4681 cccaacacca acgcggcaac tgcgcgagtg ctggatcgtc tggggatcag cgtcatgtca
     4741 gctaacgaag caggctgttg tggcgcggtg gactatcatc ttaatgcgca ggagaaaggg
     4801 ttggcacggg cgcgcaataa tattgatgcc tggtggcccg cgattgaagc aggtgccgag
     4861 gcaattttgc aaaccgccag cggttgcggc gcgttcgtca aagagtatgg gcagatgctg
     4921 aaaaacgatg cgttatatgc cgataaagcg cgtcaggtca gtgaactggc ggtcgattta
     4981 gtcgaacttc tgcgcgagga accgctggaa aaactggcaa ttcgcggcga taaaaaactg
     5041 gccttccact gcccgtgtac cctacaacat gcgcaaaagc tgaacggcga agtggaaaaa
     5101 gtgttacttc gtcttggatt taccttaacg gacgttcccg acagccatct gtgctgcggt
     5161 tcagcgggaa catatgcgtt aacgcatccc gatctggcac gccagctgcg ggataacaaa
     5221 atgaatgcgc tggaaagcgg caaaccggaa atgatcgtca ccgccaacat tggttgccag
     5281 acgcatctgg cgagcgccgg tcgtacctct gtgcgtcact ggattgaaat tgtagaacaa
     5341 gcccttgaaa aggaataaaa aaatgaaaac taaagtcatt cttagccagc aaatggcgag
     5401 tgcaattatt gccgcaggtc aggaagaggc gcagaaaaat aactggtctg tttccattgc
     5461 tgttgccgat gacggcggtc atctgctggc gttaagtcgc atggacgatt gcgcgccgat
     5521 tgcggcttac atctcccagg agaaagcgcg taccgccgcg ctggggcgtc gtgaaactaa
     5581 gggctatgaa gagatggtga acaacggacg taccgcgttc gtgactgcgc cgttactgac
     5641 gtcgctggaa ggcggcgtac cggttgttgt tgatgggcaa attattggtg ccgtgggcgt
     5701 ttctggttta accggagcac aggatgcgca ggtcgcgaaa gtggcagcag cggtgttggc
     5761 gaaataagcg aaaacgagga gagaaacaat gagtcaaacc ataacccagg gccgtttacg
     5821 cattgacgcc aattttaaac gttttgtgga tggagaagtt ttgccgggcg tggaactgga
     5881 cgctgctgca ttctggcaca atgttgatga gatcgttcac gatctggcac cagaaaatcg
     5941 tcagttgctg gcagaacgcg atcgcattca ggcggcgctt gatgagtggc atcgcagcaa
     6001 tccggggccg gtaaaagata aagcggccta taaatctttc cttcgtgaac tgggctacct
     6061 ggtgccgcaa ccggagcgcg tgacggtgga aaccacgggc attgacagcg aaatcaccag
     6121 tcaggcgggg ccacagctgg tggttccggc aatgaacgcc cgctacgcgc tgaacgcggc
     6181 gaacgctcgc tggggctcac tgtacgatgc gttatacggc agcgacatca tcccgcagga
     6241 aggggcgatg gtcagtggct acgatccgca gcgcggtgag caggttatcg cctgggttcg
     6301 acgtttcctc gatgaatcac taccgctgga aaacggcagc tatcaggatg tggtggcgtt
     6361 taaggtggtc gataaacaat tacgcatcca gttgaaaaat ggtaaagaaa ccacgttacg
     6421 tactccagca cagtttgtcg gttaccgtgg cgatgccgct gcgccgacct gcattttgct
     6481 gaaaaataac ggcctgcata ttgaactgca aattgatgcc aacgggcgga ttggcaaaga
     6541 cgatccggcg cacatcaacg atgttatcgt cgaagctgct atcagtacca ttctcgactg
     6601 cgaagattcg gtcgcggcgg ttgatgcgga agataaaatc ctgctgtacc gcaatctgct
     6661 gggcctgatg caggggactc tgcaagagaa aatggagaaa aacggtcggc aaatcgtacg
     6721 taaactgaat gacgatcgtc agtacaccgc cgccgatggc tctgaaattt ctctgcacgg
     6781 acgctcgttg ctgtttatcc gcaacgtggg tcatttgatg accattcctg tgatttggga
     6841 cagcgaaggc aatgaaatcc cggaaggtat tcttgatggc gtcatgactg gcgcgattgc
     6901 cctctatgat ttaaaagtgc agaaaaactc gcgcactggc agcgtctata ttgtgaaacc
     6961 gaaaatgcac ggtccgcagg aagtggcgtt cgccaacaaa ctgtttaccc gcattgagac
     7021 aatgctcggt atggcaccga ataccctgaa aatgggcatt atggatgaag aacgccggac
     7081 ctcgctgaac ttgcgtagct gtatcgctca ggcgcgcaac cgcgtggcgt tcatcaatac
     7141 cggtttcctc gaccgtaccg gcgatgaaat gcattcggtg atggaagctg gcccgatgct
     7201 gcgtaaaaac cagatgaaat cgacgccgtg gatcaaagcc tacgaacgta ataacgtgct
     7261 ttccggtctg ttctgtgggc tgcgcggtaa agcgcaaatc ggtaaaggca tgtgggcaat
     7321 gccggacctg atggcagaca tgtacagcca gaagggcgac caactgcgtg ccggggcaaa
     7381 cacagcctgg gttccgtcac caaccgctgc tacgctccat gcgctgcact accaccatac
     7441 caacgtacag agcgtgcaag ccaacattgc ccagaccgag ttcagtgctg aatttgaacc
     7501 gctgctggac gatctgctga ctattccggt ggctgaaaac gctaactggt cggcgcaaga
     7561 gatccaacaa gagctggata acaacgtgca ggggattctg gggtacgtgg tgcgttgggt
     7621 ggagcagggg attggttgtt caaaagtgcc ggatattcac aacgtggcgc tgatggaaga
     7681 ccgcgcaacg ctgcgtatct ccagccagca tatcgccaac tggttacgtc acggtattct
     7741 gaccaaagaa caggtgcagg cgtcgctgga gaatatggcg aaagtggttg atcagcaaaa
     7801 tgctggcgat ccggcttatc gtccgatggc ggggaatttc gctaactcgt gtgcttttaa
     7861 agctgccagc gatttaatct tcctcggcgt gaaacagcca aacggttata ccgaaccgtt
     7921 attacacgcc tggcgtttac gcgaaaaaga aagtcattaa tagcgtcaaa aaagccccgg
     7981 caagttttat tgtcggggct atgatagtaa ctgcggtagt acatttatcc cggaaataac
     8041 cggagcgatt atcagggttt aacaatatta aatacccgta gcgcattact agcgggccta
     8101 aaacaacaat atggacatct taacgatact ctgcgcctgc gtgtgcggaa cagaaaccct
     8161 tacccgatat acttgttatg ccagaggtag caattgcgtt agcggcgtac tgttgctgta
     8221 aagtataacg cctatatttt acagttcagt cctcttttgc ttattacaac cttaagtata
     8281 tcaagcatat aaagataata agagactgaa caatatggtt acctggaccc aaatgtatat
     8341 gccgatggga ggactggggc tatccgctct ggtcgccctg atcccgataa tattcttctt
     8401 cgttgcactc gcggtattac gtctgaaagg acatgtcgct ggagcaataa cccttatatt
     8461 atctatcctg attgcgatat tcgcctttaa aatgccgatt gatatggcat ttgctgctgc
     8521 gggctatggc tttatttatg gattatggcc aattgcgtgg attattgtcg cggcggtgtt
     8581 cctgtataaa ttaaccgttg ccagcgggca gttcgatatt atccgcagct cggttatctc
     8641 catcaccgac gatcagcgtt tgcaggtgtt actgattggt ttctcctttg gtgcgttgct
     8701 ggaaggagct gctggctttg gtgcgacggt ggcgtttggt gcgttgggcg tgccgattct
     8761 ggtcgccggt caggtaacgg gaatcgatcc gttccacatt ggcgcaatgg cgggacgtca
     8821 gttaccgttc ctgtcggttc ttgtgccgtt ctggctggta gcaatgatgg acggctggaa
     8881 aggggtgaaa gagacgtggc cagcggcgct ggttgctggg ggaagcttcg ctgtcactca
     8941 gttctttacc tctaactata ttggtccgga actgccggat attacttcgg cgctggtgag
     9001 tatcgtctca ctcgctttat ttcttaaagt ctggcggccg aaaaataccg aaacggcaat
     9061 cagcatgggg caatccgcag gtgcgatggt ggtaaataag ccatcttctg gcggtcccgt
     9121 gccttcagaa tatagtctgg ggcaaatcat tcgtgcgtgg tcaccgtttt taatcttaac
     9181 ggtgctggtc accatctgga ccatgaagcc gtttaaagcg ttatttgctc cgggcggcgc
     9241 gttttattca ctggtgatta atttccagat ccctcatttg catcaacaag tgttgaaagc
     9301 ggcacccatt gtcgcccaac caacgccaat ggatgcggtg tttaaattcg accctctctc
     9361 ggctggcggc accgctattt ttattgcggc gattatctct atcttcatcc tcggcgtggg
     9421 catcaagaaa gggattggcg tctttgccga aatgctaatt agtctgaagt ggccgatatt
     9481 gtcgattggc atggtgctgg cattcgcctt cgtcaccaac tattccggca tgtccaccac
     9541 gctggcgctg gtactggcag gtacaggcgt gatgttcccg tttttctcac cgtttctcgg
     9601 ctggctgggc gtattcctta ccgggtcgga cacttcttca aacgccctgt ttggttcact
     9661 gcaatcgacc acggcgcagc aaatcaacgt ctctgacact ctgctggtgg cagcaaatac
     9721 cagcggcggc gtaactggca agatgatctc cccgcaatct atcgccgtgg cctgcgccgc
     9781 gacgggcatg gtgggccgag aatctgaact gttccgctac accgtgaagc acagtctgat
     9841 ttttgccagc gttatcggcg ttatcaccct gctgcaggcg tatgtgttta ccgggatgtt
     9901 agtctcgtaa atcaataaag ccggatgaag gtaaatatca cccggctttc catttgtctg
     9961 tcgtttatct cgccagggaa ccgtttttac gattggcttc tttatacgta gtctccgttt
    10021 tacgatgcga attgtttaat atttatacaa cttggcttct gtgctggtta taaagtaccc
    10081 actttcatga gatcaatttt agtatgttat tacattgctt catctgttgc tttcatgacg
    10141 aatggcgttt tttctcttat ttatattttt cactagaaca atgagtagat caaaattccc
    10201 aaaactccca aaaacataag attattcgca aagctgattt tgcagaataa tgagaacttt
    10261 taagatttgt cgaggatcaa ttttaagcaa tttatctcca cttgttacat aaaagatgcc
    10321 gttaggttaa taagttaatc ttgctataac agtgttcgtt actgcctatt aataaagata
    10381 tttgggatag ggtaacgtgg atgaggattc gaaatcatcc gtcttgcgga aagtgaaatg
    10441 ttggcagatg ttaatattat gtatgtattt taactccaac aacacaccaa tcattttgtt
    10501 agaaataaga aaatttatgg ttaaataatt tggtgattat gaagcattaa atcgtaatgc
    10561 caaatattgt tcgctttgga gagtattatt gcctgtttgt gtctgttgag cagaaaaatc
    10621 atctgaattt gaatactttt attgggtgtg aataaatgaa aaaatcatta aaatcattaa
    10681 aatcattaaa atcattaact tatatgaata tttcagagtt ggagttcgaa tgttgacacc
    10741 tgagtatttc ctgctaaaaa tagccctgct ttacggatgg ttgtattgca ataacacgaa
    10801 agtagtaaga gattaaaggt atctgtcagc gataactctg c
//
LOCUS       DAJVPP010000120        10840 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVPP010000120 DAJVPP010000000
VERSION     DAJVPP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.120"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..265)
                     /locus_tag="OQM34_003387"
     CDS             complement(<1..265)
                     /locus_tag="OQM34_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1453704.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGK"
     gene            complement(377..1405)
                     /gene="malI"
                     /locus_tag="OQM34_003388"
     CDS             complement(377..1405)
                     /gene="malI"
                     /locus_tag="OQM34_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1453705.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1580..3172
                     /gene="malX"
                     /locus_tag="OQM34_003389"
     CDS             1580..3172
                     /gene="malX"
                     /locus_tag="OQM34_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1453706.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3182..4354
                     /gene="malY"
                     /locus_tag="OQM34_003390"
     CDS             3182..4354
                     /gene="malY"
                     /locus_tag="OQM34_003390"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1453707.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4458..5459
                     /gene="add"
                     /locus_tag="OQM34_003391"
     CDS             4458..5459
                     /gene="add"
                     /locus_tag="OQM34_003391"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1453708.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5495..6535)
                     /locus_tag="OQM34_003392"
     CDS             complement(5495..6535)
                     /locus_tag="OQM34_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1453709.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6778..6903
                     /gene="blr"
                     /locus_tag="OQM34_003393"
     CDS             6778..6903
                     /gene="blr"
                     /locus_tag="OQM34_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1453710.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7176..7391
                     /gene="ydgT"
                     /locus_tag="OQM34_003394"
     CDS             7176..7391
                     /gene="ydgT"
                     /locus_tag="OQM34_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1453711.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7477..7917
                     /locus_tag="OQM34_003395"
     CDS             7477..7917
                     /locus_tag="OQM34_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1453712.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7994..8575
                     /gene="rsxA"
                     /locus_tag="OQM34_003396"
     CDS             7994..8575
                     /gene="rsxA"
                     /locus_tag="OQM34_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1453713.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8575..9153
                     /gene="rsxB"
                     /locus_tag="OQM34_003397"
     CDS             8575..9153
                     /gene="rsxB"
                     /locus_tag="OQM34_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1453714.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9146..>10840
                     /gene="rsxC"
                     /locus_tag="OQM34_003398"
     CDS             9146..>10840
                     /gene="rsxC"
                     /locus_tag="OQM34_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1453715.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2502 a         2799 c         2743 g         2796 t
ORIGIN      
        1 ctttacccag attactgacg gcaaagtctg ccagtgcaga gagttcctgt tcggaagtaa
       61 tatcacaacg ccaggcaaat gcctgaccat ccagttgttg aatttcgtct acaacatggt
      121 tagctgcgtc ggcgttaata tcactgacca ccacagatgc gccagctgtc gcgaatgtaa
      181 tggcgatttc tttaccaata cctgcacccg cacctgtgat gatggcgcat tttccgtcga
      241 gtctcaggtt gtcagaatta aacacataaa cctcctgtga acttcagtaa aaataaaagg
      301 caccgcctgt gtgccacatt ttttatcatt aaccttgagg aaaggacact accaagtata
      361 gcagataaag agaggattat ttcgccgcaa tgagccgggc gggaataata agattgcgtg
      421 aatgcgtctc ttcatgggtg attttttgca tcatgcgatc cgcaagtgtg ataccaagtt
      481 cccgcgctgg cgtgctggcc caggtaacag gtatatcatc aagtgtggtt ggtgtcgcat
      541 cggtaaatgc cgccagcgaa acctgttgct caaagtaacg atcgactccg ctttccccgc
      601 tttgccgccc tgctttcagc aaaccaaacc atgcccccat cgcaatagtt tcgttatagc
      661 aaaccacggc actgatggtc gggttatgac gtaaaagcgc cgtgatagct tccgcggctt
      721 gcttctggct ggaagtgcac tccaacaccc aatcgctgtg aaacggcagg ccaaatttta
      781 gtagagttgc acaatagccc cccacccgtt ctgcacgggt taatgaggaa ctttgccctc
      841 ccagccaggc gatccgctga tgcccattgc gaatgagatg ctccgtcaac aactgtgcag
      901 cctgcatgtt gtccgggcga accgtatcaa catcatcaag ataactggca cgggaagcga
      961 aaatcaccgg gatagctttt tcttctgcca tccgccgcag gtcatcgctg cttcctgcag
     1021 ccccggcaat taccacacca tcgacaccct gattcagtaa cagtgaaaac cgctgtgcca
     1081 gctgctcgcc gtctttaccg ccgtgaagca aaaaaaccat ccgtccctgt gcttccagag
     1141 cttccgtcaa tccggccgtc aattcggcgt aaaacggcgc agataaatca cggacgatca
     1201 aaccaatgac gccgctttgc ccgccgcgca gcgccgacgc ctgtcgattg cgcacaaatc
     1261 ccagctcttc aatggcggcg ttaacgcgtt ctcctgtggc ggtagagatt cgccctttgc
     1321 cattaagcac cagcgaaacg gtacttaccg acacgcccgc agccagcgca acatcatgaa
     1381 tggttatttt tttggcggta gccatgcaga tgacctactc cctgattatg tgacagataa
     1441 aacgttttac cttttattta tcttataccc gctattatcg ttgcgtaatg tgatttatga
     1501 ctcactaaaa tttgataaaa cgttttatct tctcgcgcaa tttactgaat ccagattgtt
     1561 ctctacgagg agtcgtttta tgacagcgaa aacagcaccg aaagtcacgc tgtgggagtt
     1621 cttccagcag ttaggcaaaa ccttcatgtt acccgtggca ttattgtcgt tctgcggcat
     1681 tatgctcggc attggtagtt ctcttagcag ccatgatgtc ataaccctga tcccggtcct
     1741 gggcaacccc gtgttgcagg ctatctttac ctggatgagt aagattggct cgtttgcttt
     1801 tagtttcctg cctgtcatgt tctgtatcgc catcccgctg ggcctggcac gcgaaaataa
     1861 aggcgtagcg gcattcgctg gcttcgtcgg ttatgcggta atgaacctcg cggtaaactt
     1921 ctggttgacc aataaaggca ttctgccaac cacagatgcc gccgttctga aagccaataa
     1981 catccagagc attcttggga tccagtcgat cgataccggg atcctcggtg cggtgatcgc
     2041 cggtattatc gtctggatgc tgcatgagcg tttccataat atccgcctgc cggatgcgct
     2101 ggcattcttc ggcggtacgc gcttcgtacc aattatctcc tcgctggtga tgggccttgt
     2161 cggcctggtg attccattag tctggccgat tttcgccatg ggtattagcg gcttgggcca
     2221 tatgataaac agcgcgggtg atttcggacc gatgctgttt ggtaccggtg aacgtctgct
     2281 gttgccgttt ggtctgcatc acattctggt ggcattaatt cgctttaccg acgcaggcgg
     2341 cacgcaggag gtctgcggtc aaaccgtcag cggcgcactg accatcttcc aggcgcaatt
     2401 gagttgcccg accactcacg gtttttctga aagcgtcacg cgtttccttt cgcaaggtaa
     2461 aatgcctgcg tttctcggcg gtctaccagg tgcagcgtta gctatgtatc actgcgcgcg
     2521 cccggaaaat cgccataaaa ttaaaggtct gctgatttct ggcctgatcg cctgcgtcgt
     2581 tggcggcact accgaaccgc tggaattcct gttcctgttc gtagcgccag ttctgtatgt
     2641 catccacgcg ctgttaaccg gcctcggctt caccgtcatg tctgtgctcg gcgtcaccat
     2701 cggtaatacc gacggcaata tcatcgactt cgtggtgttc ggtattttgc atggtctgtc
     2761 aaccaagtgg tacatggtgc cagtggtggc ggcaatctgg tttgtcgttt actacgtcat
     2821 cttccgtttc gctatcaccc gcttcaatct gaaaaccccg gggcgcgata gcgaagttgc
     2881 cagctcaatc gaaaaagccg ttgccggtgc gccgggtaaa tcaggttaca acgttcctgc
     2941 aatcctcgaa gcattaggcg gtgccgacaa tattgtcagc ctcgataact gcattacccg
     3001 tctgcgtttg tctgtggaag atatgtcgct tgttaatgtg caggcactga aggacaatcg
     3061 ggcaattggc gtagtacaac ttaatcaaca taacctgcag gttgttatcg ggccacaagt
     3121 tcagtcagta aaagatgaaa tggccggtct gatgcatact gtccaggcat aaggataaga
     3181 tatgttcgat ttttcaaagg tcgtggatcg tcatggcaca tggtgtacac agtgggatta
     3241 tgtcgctgac cgtttcggca ctgctgacct gttaccgttc acgatttcag acatggattt
     3301 tgccactgcc ccctgcatta tcgaggcgct gaatcagcgc ctgatgcacg gcgtatttgg
     3361 ctacagccgc tggaaaaacg atgagtttct cgcggctatt gcccactggt tttccaccca
     3421 gcattacacc gcaattgata cgcagacggt ggtgtatggc ccttctgtca tctatatggt
     3481 ttcagaactg attcgtcagt ggtctgaaac aggtgaaggc gtggtgatcc acacgcccgc
     3541 ctatgacgca ttttacaagg ccattgaagg taaccagcgc acagtaatgc ccgttgcttt
     3601 agagaagcag gctgacggct ggttttgcga tatgggcaag ctggaagccg ttttggcgaa
     3661 accagaatgt aaaattatgc tcctgtgtaa cccacagaat cctaccggga aagtgtggac
     3721 gtgcgatgag ctggagatca tggctgacct gtgcgagcgt catgatgtgc gggttatttc
     3781 cgatgaaatc catatggata tggtctgggg cgagcagccg catattccct ggagtaatgt
     3841 ggcgcgggga gactgggcgt tgctgacatc gggatcgaaa agtttcaata ttcccgcgct
     3901 gaccggtgct tacgggatta tagaaaatag cagcagccgt gatgcctatt tatcggcact
     3961 gaaaggacgt gatgggcttt cttccccttc ggtactggcg ttaactgccc atatcgccgc
     4021 ctatcagcaa ggtgcaccgt ggctggatgc cttacgcgtc tatctgaaag ataacctgac
     4081 gtatatctca gataaaatga acgccgcgtt tcctgaactc aactggcaga tcccgcaatc
     4141 cacttatctg gcctggcttg atctacgtcc gttgaatatt gacgacaacg cgttgcaaaa
     4201 agcgcttatc gagcaagaaa aagtcgcgat catgccgggg tatacctatg gtgaagaagg
     4261 tcgtggtttt gtccgtctca atgccggctg cccacgttcg aaactggaaa aaggtgtggc
     4321 tggattaata aacgccatcc gcgctgttcg ctaaccccaa ttgcgcaacg taaaaaatcg
     4381 ttgcgcaatc gtggattttt accctgcttt gtttttataa tggtgcgcac ttttatatcc
     4441 agaaaaagag tgcgaccatg attgatacca ccctgccatt aactgatatc catcgccacc
     4501 ttgatggcaa cattcgtccc cagaccattc ttgaacttgg ccgccagtat aatatctcgc
     4561 ttcctgcaca atcccttgaa acactgattc cccacgttca ggtcattgcc aacgaacccg
     4621 atctggtgag ctttctgacc aaacttgact ggggcgttaa agttctcgcc tctcttgatg
     4681 cctgccgccg cgtggcattt gaaaacattg aagatgcagc ccgtaacggc ctgcgctatg
     4741 tcgagctgcg tttttcacca ggctacatgg caatggcaca taagctgcct gtagcgggtg
     4801 ttgtcgaagc ggtgatcgat ggcgtacgtg aaggttgccg cacctttggt gtgcaggcga
     4861 agcttatcgg cattatgagc cgaaccttcg gcgaagccgc ctgtcagcaa gagctggagg
     4921 cctttttagc ccaccgtgac cagattaccg cacttgattt agctggtgat gaacttggat
     4981 tcccgggaag tctgttcctt tctcacttca accgcgcgcg tgatgcgggc tggcatatta
     5041 ccgtccatgc aggcgaagct gccgggccgg aaagcatctg gcaggcgatt cgtgaactgg
     5101 gtgcggagcg tattggacat ggcgtaaaag ccattgaaga tcgggcgctg atggattttc
     5161 tcgccgagca acaaattggt attgaatcct gtctgacctc caatattcag accagcaccg
     5221 tagcagagct ggctgcacat ccgctgaaaa cgttccttga gcatggcatt cgtgccagca
     5281 ttaacactga cgatcccggc gtacagggag tggatatcat tcacgaatat accgttgccg
     5341 cgccagctgc tgggttatcc cgtgagcaaa tccgccaggc acagattaat ggtctggaaa
     5401 tggctttcct cagcgcagag gaaaaacgcg cactgcgaga aaaagtcgcc gcgaagtaac
     5461 aaaaatggat ggtgcaaatt gcaccatcca tttttcacgc aaggcacaac gtcgcgcgat
     5521 gtttggcgga ttcgatgccc agctcaatca actccattac ctggattgcc tggcttgccg
     5581 gaaccggatt ttcaccatcg ccatttaacg catcacgaat cgccgcatag taagccggat
     5641 aattcccagg caccgtcaac agcgtttctt cgacacgttc ctcaccttcc acgcgggtaa
     5701 gtacgccatc acgcatatcg tagccccagt cttcctgcgg cagacgctcg ccatttttca
     5761 gacgttcttc ctgtggatcg aggccatatt tcacataact gcctcgggat ccatgcacga
     5821 tataccgtgc tgactcagcg gctgccagca tggtaccgtg taaaatgact cgccgctgtg
     5881 gataggacaa gatggcgtgg aaataatcgg tcgactgcgc tccgggccgt aactgtgcca
     5941 aatctaccgt catgctgacc ggtaaaccaa atagcgtaat ggcctgatca agaagatgcg
     6001 gtgctaaatc gtaccagata ccgctgcctg ggccgccctg ttcacgccaa cgatcgcgca
     6061 cctgcggacg gaagcggtca aaatgagact caaagtaagc aacttcaccc agcacgcctt
     6121 ccgcgagcaa accttttagc gtcaagaaat cgctatccca acgacggtta tggaatacag
     6181 acagcacacg ccccaggctt tttgccagcg catccagctc tcgcgcttgt gacagtgtca
     6241 cggtaaaggg tttatcaacg accacatatt tacccgcctc aagcgccgct ttggctaacg
     6301 ggaaatgggt atcgttgggt gtaggaatga caatcaggtc tatgttggga tcgttaaaca
     6361 gatgcttcgg ctcagagaca accgtaaccg ttggccagtc ggcttttact tttgtttcat
     6421 cactgctgga gattaccgcc agttccagcc cgggcgtgcc cgcaatcagg ggcgcatgga
     6481 aggttttgct cgcataacca tacccaatca acccaacacg gatgttgtcg ctcatgtcat
     6541 tgcctctcat tgaagtatga tggctatttg acactatcct ttacccacgc tcaacagttt
     6601 aataacctgc cagcaataag ggatgttgtt taacttaagt caaaaaaata gcgaattttc
     6661 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6721 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6781 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6841 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6901 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6961 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     7021 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     7081 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac taataatacc
     7141 tacccctttg gtattaatta ttttatggac cttttatgac tgttcaggac tacttattaa
     7201 aatttcgcaa aatcagttca ctcgaaagtc tggaaaaact ctacgaccat cttaattaca
     7261 ccctgacgga cgatcaggaa ctgatcaata tgtatcgtgc tgccgatcac cgtcgcgcag
     7321 agctggtttc tggcgggcgt ttgtttgacc tcggccaggt accgaagtcc gtctggcact
     7381 atgtccaata aagaaagtag cgatcggtta taaaagcctt ataataacgc ccctaaaatt
     7441 agatttgcct gtttgcgtcg ctattctgga gaacctatga ccaccacaac gccacaacgt
     7501 attggaggct ggttgcttgg cccgttggcc tggcttttag tcgctctgtt gagtacgacg
     7561 ctggcgttgt tgctgtatac cgctgcgtta tcttctccgc aaacatttca gacgctcggc
     7621 ggacaagcac tgacgacaca aatcttatgg ggcgtttctt ttattaccgc tatcgccatg
     7681 tggtattaca cactgtggtt gaccatcgct ttctttaaac gtcggcgttg tgttcctaaa
     7741 cactatatta tctggctgct tatttccgta ttgctggcag taaaagcatt cgctttttca
     7801 cccgtggaag ttggcatagc cgttcgccag ttgctgttta ctttgttggc aaccgcattg
     7861 atcgtcccct atttcaaacg ctcgtcgcgg gtaaaagcga cgtttgtgaa tccgtaataa
     7921 ccttacagtt aacctgttgt cgcctgctct ggattaacgg ataataggcg gcttttttat
     7981 ttcaggccga aaaatgactg actacctgtt actgtttgtc ggaactgtac tggtcaataa
     8041 ctttgtactg gtcaagtttc tcggtctctg tccgtttatg ggggtttcca aaaaactgga
     8101 aaccgcgatg ggcatggggc tggcaacaac gtttgtgatg acgctgacgt ctatttgcgc
     8161 ctggcttatc gatacgtgga ttttgatccc acttaatctg atttacctgc gcaccctggc
     8221 atttattctg gtgattgctg tggtcgtgca gttcaccgag atggtggtgc gcaaaaccag
     8281 cccggtgctt taccgtctgc tggggatttt tttgccgctt atcaccacca actgtgcagt
     8341 gctcggcgtg gcgttgctga atatcaatct cgggcacaat ttcttgcagt cggcgctgta
     8401 cggtttttcc gccgctgtcg gtttctcgct ggtgatggtg ctcttcgccg ccatccgcga
     8461 acgccttgct gtggctgatg tcccggcacc ttttcgcggt aatgccattg cgttaattac
     8521 cgcaggtctt atgtctctgg cctttatggg ctttagtggt ttggtgaagt tgtaatgaat
     8581 gctatctgga ttgccgttgc cgccgtgagc ctgctgggcc tggcgtttgg cgccattctg
     8641 ggttatgcct cccgccgttt tgcggtggaa gacgatccgg tcgttgagaa aattgacgaa
     8701 atcttaccgc agagccagtg tggtcagtgc ggttatcccg gctgtcgccc ctacgcggaa
     8761 gccatcagct gtaacggtga aaaaatcaac cgttgcgccc caggtggcga agctgtgatg
     8821 ctaaaaattg ccgagttgct taatgtcgag ccgcagccgc tggatggcga agcgcaagag
     8881 ctaacgcctg cgcggatggt ggcggttatt gatgaaaata actgtattgg ctgcactaaa
     8941 tgtattcagg cttgtccggt agacgccatc gttggcgcta cccgtgtcat gcatacggta
     9001 atgagtgatc tctgtacggg ctgcaattta tgtgttgatc cgtgcccgac gcactgcatc
     9061 tcgttgcaac cggtcgcaga aacacctgac tcctggaaat gggatctgaa caccattccc
     9121 gtgcgtatca ttcccgtgga acaccatgct taagttattc tctgcattca gaaaaaataa
     9181 aatctgggat ttcaacggcg gcatccatcc accggagatg aaaacccaga ccaacggtac
     9241 ccccctgcgc caggtactac tggcgcagca ttttgttatt ccactgaaac agcatattgg
     9301 cgctgaaggt gagttgtgcg ttagcgtcgg cgataaagta ttgcgcggcc agccgcttac
     9361 ccgtggtcgc ggcaaaatgc tgcctgttca cgcgcccacc tcgggtaccg ttacggctat
     9421 tgcgccccac tctacggctc atccttcagc tttagctgaa ttaagcgtga ttattgatgc
     9481 cgatggtgaa gactgctgga tcccgcgcga cggctgggtc gattatcgct ctcgcagtcg
     9541 cgaagagtta atcgagcgca tacatcagtt tggtgttgcc gggctgggcg gtgccggatt
     9601 cccgacaggc gttaaattgc agggtggtgg agataagatt gaaacgttga ttatcaacgc
     9661 tgctgagtgc gagccgtaca ttaccgccga tgaccgtttg atgcaggatt gcgcggctca
     9721 ggtcgtagag ggtattcgca ttcttgcgca tattctgcag ccacgcgaaa ttcttatcgg
     9781 cattgaagat aacaaaccgc aggcgatttc catgctgcgc gcggtgctgg cggactctaa
     9841 cgatatttct ctgcgggtga ttccaaccaa atatccttct ggcggtgcta aacaattaac
     9901 ctacattctg accgggaagc aggttccaca tggcgggcgt tcatccgata tcggcgtatt
     9961 aatgcaaaac gtcggcactg cttatgcagt gaaacgtgcc gttattgatg gcgagccgat
    10021 taccgagcgt gttgtaaccc tgactggcga agcaatcgct cgcccgggca acgtctgggc
    10081 acggctgggg acgccagtgc gtcatttatt gaatgatgcc ggattctgcc cctctgccga
    10141 tcaaatggtg attatgggtg gcccgctaat gggctttacc ttgccatggc tggatgtccc
    10201 ggtcgtaaag attaccaact gtctgttggc tccctctgcc aatgaacttg gcgaaccaca
    10261 ggaagaacaa agctgcatcc ggtgtagcgc ctgtgctgac gcctgccctg tggatctttt
    10321 gccgcaacag ttgtactggt tcagcaaagg tcagcaacac gataaagcta ccacgcataa
    10381 cattgccgac tgcattgaat gtggggcttg cgcgtgggtt tgcccgagca atattccctt
    10441 ggtgcaatat ttccgtcagg aaaaagctga aattgcggct attcgtcagg aagaaaagcg
    10501 cgccgcagaa gccaaagcgc gtttcgaagc gcgccaggct cgtctggagc gcgaaaaagc
    10561 gactcgcctt gaacgacata agagcgcagc cgttcaacct gcagccaaag ataaagatgc
    10621 gattgctgcc gctctggcgc gggtgaaaga gaaacaggcc caggctacac agcctattgt
    10681 gattaaagcg ggcgaacgcc cggataacag tgcaattatt gcagaacggg aagcccgtaa
    10741 agcgcaagcc agagcgaaac aggcagaact ggagcaaact aacgacgcag caaccgttgc
    10801 tgaaccacgt aaaactgccg ttgaagcagc tatcgcccgc
//
LOCUS       DAJVPP010000121        10591 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVPP010000121 DAJVPP010000000
VERSION     DAJVPP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.121"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            144..494
                     /locus_tag="OQM34_003399"
     CDS             144..494
                     /locus_tag="OQM34_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCS1453716.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            547..942
                     /locus_tag="OQM34_003400"
     CDS             547..942
                     /locus_tag="OQM34_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS1453717.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            983..1726
                     /gene="cmoA"
                     /locus_tag="OQM34_003401"
     CDS             983..1726
                     /gene="cmoA"
                     /locus_tag="OQM34_003401"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1453718.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1723..2694
                     /gene="cmoB"
                     /locus_tag="OQM34_003402"
     CDS             1723..2694
                     /gene="cmoB"
                     /locus_tag="OQM34_003402"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1453719.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2859..5288)
                     /gene="torZ"
                     /locus_tag="OQM34_003403"
     CDS             complement(2859..5288)
                     /gene="torZ"
                     /locus_tag="OQM34_003403"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCS1453720.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLSAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5313..6413)
                     /locus_tag="OQM34_003404"
     CDS             complement(5313..6413)
                     /locus_tag="OQM34_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1453721.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6801..7547)
                     /gene="cutC"
                     /locus_tag="OQM34_003405"
     CDS             complement(6801..7547)
                     /gene="cutC"
                     /locus_tag="OQM34_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1453722.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7561..8127)
                     /locus_tag="OQM34_003406"
     CDS             complement(7561..8127)
                     /locus_tag="OQM34_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1453723.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8343..10076
                     /gene="argS"
                     /locus_tag="OQM34_003407"
     CDS             8343..10076
                     /gene="argS"
                     /locus_tag="OQM34_003407"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1453724.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10253..>10591
                     /locus_tag="OQM34_003408"
     CDS             10253..>10591
                     /locus_tag="OQM34_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCS1453725.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELIL"
BASE COUNT         2575 a         2718 c         2542 g         2756 t
ORIGIN      
        1 ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact catagctaac
       61 cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta ggaaccatct
      121 gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt catgcatcac
      181 gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg
      241 ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat atgacgcaaa
      301 accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag accactacgc
      361 cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta cataccctgt
      421 gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt ccacagcaat
      481 cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat tttaaaggga
      541 gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat gaagttctct
      601 tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg tggttttagc
      661 gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc catcgcgatt
      721 gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca tatttgcggc
      781 atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg tctgtttcgc
      841 tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat
      901 ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat acgccacttt
      961 ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg cccctatcgc
     1021 cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg atatgatcca
     1081 gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag ccgagcgctt
     1141 cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg cgacgctctc
     1201 ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca actccccggc
     1261 gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc cagtagacgt
     1321 cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg tgctgaattt
     1381 taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa tttatcaagg
     1441 actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag atgccaaagt
     1501 tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt acagcgaact
     1561 ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt ccgtggaaac
     1621 ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt tccagtgctt
     1681 taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga ctttggtaac
     1741 ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct gcccgcgcag
     1801 attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc caacacggtg
     1861 gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt aaccgccgaa
     1921 agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat gcggaacctg
     1981 atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac cgaatggcgt
     2041 tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg acgcaccatt
     2101 ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc aggcgcgcac
     2161 ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc ggtgcgtaaa
     2221 ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca gcttccggca
     2281 ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg ttcgccgctg
     2341 gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt gctggaaacg
     2401 ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta cgcgcaaatg
     2461 cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa gaagtgtggt
     2521 tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca gcgacgcact
     2581 gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc gagtaagaca
     2641 gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc gtaaaggtct
     2701 ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct tgctaattca
     2761 atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt tttacgtttg
     2821 cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac agcaggctta
     2881 tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac cagcgcagag
     2941 ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa cacgttcgca
     3001 ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg cacgcatacc
     3061 acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc acgtttgttc
     3121 cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc ggtgtgaata
     3181 gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata gttaagctga
     3241 ctgtgtaaac ggtgtgccgg atgtgcgctc agaagctgca actgcttctc gtcggcggta
     3301 cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt atagccaaat
     3361 ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag cgcatttttc
     3421 accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc gctgcggcgc
     3481 atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt gacgcgttgc
     3541 gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat ttcatcttta
     3601 ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc ggcaaacacg
     3661 tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg cacaatatgc
     3721 tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt ggtgatcggt
     3781 aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac gacgatcatc
     3841 tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt aaagttaccg
     3901 ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc gttatgctga
     3961 tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat cgccgtcatt
     4021 ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat ttccggcagc
     4081 acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa accaaaaccg
     4141 ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat ccagtgtttc
     4201 tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt gcgattcgcc
     4261 gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat ttccgccgtc
     4321 cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc aaactgcgga
     4381 tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt catcagggta
     4441 tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca
     4501 ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat cactggtttg
     4561 ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact gctccaggca
     4621 atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct gttttccaga
     4681 atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac atgcggcatg
     4741 atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc gcccgccagg
     4801 ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg
     4861 taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc atgttgctca
     4921 tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc gccacgacct
     4981 ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg ctgaatacgc
     5041 gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat ggttttcgcc
     5101 agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat cgctccccaa
     5161 cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag cggtgctgcc
     5221 gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc acgtcttgtt
     5281 aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat atctttggcg
     5341 ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct ggttcgtgcc
     5401 cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc ggcaatagag
     5461 gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca tagcggttta
     5521 cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg ccattgattg
     5581 ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc ttcttcactt
     5641 aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc actgccctgc
     5701 atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac gacggtgaga
     5761 tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa aggataaata
     5821 tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc cgcgttgtca
     5881 tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat acaggtttcg
     5941 cctccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga ggcaatatcc
     6001 atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt taattctttc
     6061 catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact gtctattttg
     6121 ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa taaataatcc
     6181 atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt ctggttcgaa
     6241 aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca ggaaaggcaa
     6301 aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc gccgccacca
     6361 accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg tatagattcc
     6421 tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac gaattaaccg
     6481 cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga tttattacaa
     6541 tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata tttatcagtt
     6601 ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc acaacgctat
     6661 gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac tgctcaggga
     6721 taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc atattgggcg
     6781 acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc tttcatttca
     6841 gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc atctgatgac
     6901 atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc cgcggagcta
     6961 tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg gactcctgct
     7021 ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt tgataaacct
     7081 tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa ttccgcaaga
     7141 ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa agtcactgcc
     7201 agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt cccgtcaaca
     7261 tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg cacatcctca
     7321 agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg gcgaattatc
     7381 ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa cgacggcgtt
     7441 aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt ttgctgcgcc
     7501 gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt tgctccttaa
     7561 ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa acttaatagt
     7621 tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct caccttttgg
     7681 tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa gcaatgccag
     7741 cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat gttcccaacc
     7801 ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa actgccagtg
     7861 cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat tgatcatttt
     7921 ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag cggtgacgtt
     7981 ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca accccagacg
     8041 gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa tatcctgcag
     8101 ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa aagcgttaat
     8161 ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga tgcaatagtg
     8221 aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca gtatactccc
     8281 gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg taaggtattc
     8341 cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt gcggcaggcg
     8401 cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc ggcgactatc
     8461 aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga caattagcag
     8521 agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag atcgccggtc
     8581 caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt cagcaggcgc
     8641 tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg gttgactact
     8701 ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc attattggtg
     8761 acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca aaccacgtcg
     8821 gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag cagcaggaaa
     8881 acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt
     8941 acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg
     9001 gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg acgcagaacc
     9061 agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg ggcgaaagcc
     9121 tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt ctggcagtag
     9181 aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa ggcgaaccga
     9241 tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact gatatcgcct
     9301 gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac atcgactccc
     9361 gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc tatgtaccgg
     9421 aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac ggcaaaccgt
     9481 tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa gccctggagc
     9541 gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag ctggaaaaac
     9601 tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa aaccgcacca
     9661 cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc gcgccataca
     9721 tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt aacgaagagc
     9781 aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg gcagctcgcc
     9841 tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg catgtaatgt
     9901 gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac tgcccgatcc
     9961 tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa ctgacagcga
    10021 agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt atgtaatcga
    10081 tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta ttgccatcta
    10141 aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga ggccagaagc
    10201 gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg aagtgtttaa
    10261 atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag atacggttat
    10321 agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt cagatgatga
    10381 tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg tcgatgcgca
    10441 attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac gcacaggtgc
    10501 aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag cctggctgga
    10561 ttttagggac aaagaatgtg aattaatcct t
//
LOCUS       DAJVPP010000122        10467 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVPP010000122 DAJVPP010000000
VERSION     DAJVPP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.122"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..652)
                     /locus_tag="OQM34_003409"
                     /pseudo
     CDS             complement(1..652)
                     /locus_tag="OQM34_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
     gene            1028..2668
                     /gene="trg"
                     /locus_tag="OQM34_003410"
     CDS             1028..2668
                     /gene="trg"
                     /locus_tag="OQM34_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCS1453726.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2706..3629)
                     /locus_tag="OQM34_003411"
     CDS             complement(2706..3629)
                     /locus_tag="OQM34_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1453727.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3846..5189
                     /locus_tag="OQM34_003412"
     CDS             3846..5189
                     /locus_tag="OQM34_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1453728.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5414..7069
                     /gene="opgD"
                     /locus_tag="OQM34_003413"
                     /gene_synonym="mdoD"
     CDS             5414..7069
                     /gene="opgD"
                     /locus_tag="OQM34_003413"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1453729.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            7209..7433
                     /locus_tag="OQM34_003414"
     CDS             7209..7433
                     /locus_tag="OQM34_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1453730.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7496..8032
                     /gene="rimL"
                     /locus_tag="OQM34_003415"
     CDS             7496..8032
                     /gene="rimL"
                     /locus_tag="OQM34_003415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1453731.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(8027..8994)
                     /locus_tag="OQM34_003416"
                     /pseudo
     CDS             complement(8027..8994)
                     /locus_tag="OQM34_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9118..10110
                     /gene="tehA"
                     /locus_tag="OQM34_003417"
     CDS             9118..10110
                     /gene="tehA"
                     /locus_tag="OQM34_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1453732.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMSIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            10107..>10467
                     /gene="tehB"
                     /locus_tag="OQM34_003418"
     CDS             10107..>10467
                     /gene="tehB"
                     /locus_tag="OQM34_003418"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1453733.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANM"
BASE COUNT         2660 a         2468 c         2641 g         2698 t
ORIGIN      
        1 tttttaacgt ccgcatactt gtcaggcttg ctgagagcct ttagatggta ataaaacgta
       61 ctgcgcggta tctccgcagc cctgagaagc tcatcaagag gataaaactg ccttagctcg
      121 ttgagtactt tcactttttc gtgggatgag ctaaggcttt cagctttttt agatacataa
      181 gccgcgtttc aagaaatcga acttgccttt caagatcctc aatgcgtcgg tcttttgaca
      241 gctccaatgc tgatgccgct ttttctggat caactgatat tgcaatgttt cttttggtgc
      301 caatcttgag cgcgcgtaaa ccagcttctc cgcgctcttc atagaccttc agccacctgg
      361 ctacagaacc actaccagca agcataaagt gagcagcagc ctgattaagg gacatgtgct
      421 gctcgatcac agctttcacg accttaatac gcaactctgg atcagcacta acgcctttag
      481 gtttgggaat taaacctttt tctccatgtt tttcatagag ggcaacccat gtcctgacct
      541 gggttcgggg gacaccaaaa cgtgccgaga tgatcctgta accatcatca gtagtgaagt
      601 agtgattcac gacttcaagg cgcttttcaa aagggtattt tggctttgac atattagggg
      661 ctattccatt tcatcgtcca acaaaatggg tgcagtacag ttgatagaaa tatcggtcgg
      721 ggccttcgtc tttctgattc ccggttagcc tgaaaacaga aagtctcagg cacccgcagg
      781 catcctatga ggtttcctta gggacgaaaa taatcacttc acgaaattgc gtgctgtttt
      841 ccagaatttt tcgtcattcg ggttagccag tttagccatt cgttactctc ttcattccaa
      901 tagcattaat tttctctgca ataattgttg taaaaatgtg acgcaaagag gtttttggtc
      961 ataagtaatt accgtcaagt gccgatgact ttctatcagg agtaaacctg gacgagagac
     1021 aacggtaatg aatacaactc cctcacagcg attaggtttt ttgcatcaca tcaggctggt
     1081 tccgttattt gcctgcattc ttggcggtat cttagttcta ttcgcattaa gttctgctct
     1141 ggctggctat ttcctctggc aggccgatcg cgatcagcgt gatgttactg cggagattga
     1201 gattcggacc gggttagcga acagttcaga ttttttgcgt tcagcccgga tcaatatgat
     1261 tcaggccggg gctgcgagtc gtattgcgga aatggaagca atgaagcgaa atattgcgca
     1321 agccgaatcg gagattaaac agtcgcagca aggttatcgt gcttatcaga atcgaccggt
     1381 gaaaacacct gctgatgaag ccttcgacac tgaattaaat caacgctttc aggcttatat
     1441 cacgggtatg caacctatgt tgaaatatgc caaaaatggc atgtttgaag cgattatcaa
     1501 tcatgaaagt gagcagatcc gaccgctgga taatgcttat accgatattt tgaacaaagc
     1561 cgttaagata cgtagcacca gagccaacca actggcggaa ctggcccatc agcgcacccg
     1621 cctgggtggg atgttcatga ttggcgcgtt tgtgcttgcc ctggtcatga cgctgataac
     1681 atttatggtg ctacgtcgga tcgtcattcg tccactgcaa catgccgcac aacggattga
     1741 aaaaatcgcc agtggcgatc tgacgatgaa tgatgaaccg gcgggtcgta atgaaatcgg
     1801 tcgcttaagt cgtcatttac agcaaatgca gcattcactg gggatgacag tagggactgt
     1861 tcgacagggc gcggaagaga tttatcgtgg caccagcgaa atttcagctg gcaatgcgga
     1921 cctgtcatct cgcaccgaag aacaagcggc ggctatcgaa caaactgccg ccagcatgga
     1981 gcaactcact gcgacggtga aacagaatgc ggataacgcg catcatgcca gcaaactggc
     2041 gcaagaggct tctattaaag ccagcgatgg cgggcagacg gtttccggtg tagtaaaaac
     2101 gatgggcgct atctccacga gttcgaagaa aatttctgag atcaccgccg tcatcaacag
     2161 tattgctttc cagacgaata ttctggcact gaatgctgcc gttgaagccg cgcgagcggg
     2221 tgagcaaggg cgtggatttg ccgttgtcgc cagcgaagta cggacactcg caagccgcag
     2281 cgctcaggca gcgaaagaga ttgaaggctt gatcagtgaa tcagtcaggt taattgacct
     2341 ggggtcggat gaggtggcaa cggcagggaa aaccatgagc actattgttg atgccgtcgc
     2401 gagtgtcaca catatcatgc aggaaatcgc cgccgcctcg gatgaacaaa gtagaggcat
     2461 aactcaggtt agccaggcga tttctgaaat ggataaggtg acgcaacaga atgcttctct
     2521 ggtagaagag gcctcagcag cggcggtgtc ccttgaagaa caggctgcac gattaactga
     2581 ggcggtgaac gtattccgtc tgaacaaaca ttctgtgtcg gcagaacctc gcggagcggg
     2641 tgaaccagtt agtttcgcta cggtgtgaaa atgttcaagg agggatcgac agatcccttc
     2701 acctttcaga acggcattga ttttcgaata gcgttaatca tcaactggca accagaagag
     2761 aacgtcgcat ctacgcgggt cagtattcca atcggttcgc ctgcaccatg tcccggaaca
     2821 ggcagggcca ccagcgtggc atgacgcagg tcgtctttta cagcgccaga agggacaaac
     2881 cacacgtaat cgtattcaac ggtaagttga cgagatagcg aagtagacag cgtttcgata
     2941 caacccgaag gaattttaca gccctggctc tgcactaatg catctgaatg ctggcgtggc
     3001 gcagtgcctt ctggtgatac aacgaccggc cattccagca cccggcttag cgttacgttc
     3061 tcctgaagta gcgggtgatt agggcggaca accagcttca acgattcaag aaacagcagt
     3121 tcgtaattaa gcccggtcat cagttcagga tctgacatcc gaccaatgcc gatatcgatt
     3181 tccccggttt ttaaacccgc cagaatcata gggttactca ttgtcgcaac ttgcaaggtc
     3241 gtctcttttt gttgctgatg aaactgacct ataaccgaag gtaatatccc cagtgccgca
     3301 gtaggtagtg caccaaccct gacgacatca ttattaagac cttctttacg atgaagcgac
     3361 tgtccggcag tgttgatggc gtcaagaact ctgactgcat gcgttaaaaa ttgttcgccg
     3421 ggtaaggtaa gttgcgcccc ctgacgacca cgctcaaaca agcgagcgcc cgtcagctgc
     3481 tccagttcat tcaatgtatt agagagcgca ggttgactca aattaagggt ttcagctgcg
     3541 cgccccaaag ttccttgttg tgcgacagct acgaatgtat gaaggtggcg caaacggatg
     3601 cgctgactaa acagactatt tttttccata agcgatgtta aaaacgaagc ggtgtcgctg
     3661 acaagtgaag ttgtttgatt atgataactt gattgcaaaa tattattaat aattaaagca
     3721 attatgttac agcaaaatgg ataatattga tgttttcgcg gcgagatcac agtttgtaaa
     3781 ttcttcccgc cagagtgaat gcggttacct acactccaga ttactgacca ctggaggcag
     3841 acactatggc gaacagcatc acggcggatg agattcggga acagttttcg caggcaatgt
     3901 cagccatgta ccagcaagaa gttccgcaat atggcacgct gctggaactg gtagctgatg
     3961 tgaatctggc tgtgctggaa aacaatcctc aactgcacga aaaaatggta aatgcagacg
     4021 agctggcgcg actgaatgtt gaacgtcatg gggcgattcg cgttgggact gcacaagagc
     4081 ttgctactct tcggcggatg tttgccatta tggggatgta cccggtgagc tattacgatc
     4141 tctcgcaggc aggggtgccg gtacattcga cagcatttcg gcccattgat gatgcttctc
     4201 tggcgcgtaa tcccttccgc gtttttacat ccttactccg ccttgagctt atcgagaacg
     4261 aaattttgcg ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc accccacgtt
     4321 gtcgacaact gttagaggaa tatgagcagc agggcggttt taacgaaaca caggcacagg
     4381 agtttgtgca ggaagccctg gaaacgtttc gctggcacca gttagcaacg gtagatgaag
     4441 aaacctatcg cgccttgcac aacgaacatc ggttgattgc tgatgtggtc tgttttcctg
     4501 gatgccatat caaccacctg acgccacgta cgctggatat tgaccgggtg cagtcgatga
     4561 tgcctgaatg cggaattgaa cccaaaattc tcatcgaggg gccgccgcgc cgcgaggtac
     4621 cgattttact acgccagacc agctttaaag cactggaaga gacggtgttg tttgcggggc
     4681 agaaacaggg cacgcatacc gcgcgctttg gtgaaattga gcagcgtggc gtggcattaa
     4741 cgccgaaagg gcgacaactg tatgatgatc ttctgcgtaa cgctggaacc gggcaggata
     4801 atctcactca ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac agtgagtttt
     4861 taatgcgtca gcaagggttg gcatggttcc ggtaccgtct gacgccttcg ggtgaggcgc
     4921 atcgtcaggc gattcatcct ggagacgatc cacagccctt aattgaacgt ggttgggtag
     4981 tggcgcaacc catcacctat gaagatttct tgcccgttag cgcggcgggg atcttccagt
     5041 caaatctggg taatgaaacg cagacacgca gtcacggtaa tgccagtcgc gaagcatttg
     5101 agcaggcgtt gggttgtccg gttttggatg agttccagct ttaccaggaa gcggaagaac
     5161 gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc
     5221 ctgattttac aaacgaatca ggctcatccc atcgacataa aaaaaatgcc gatttatgca
     5281 tattctctca gttcaacaat tggattatta ataaatattg tctagagtga gcggtcataa
     5341 ataagcactt tcttgccgct gaaaacgacc agcgcgggac cattcacaac accagaagga
     5401 ctcactttca ggtatggatc gtagacgatt tattaaaggt ttaatggcta tggccgccgt
     5461 gtgcggtacc agcggcattg cttctctttt ttctcaggca gcattcgcgg cagattctga
     5521 tattgccgac gggcaaaccc agcgttttga cttctccatt ctacagtcaa tggcgcacga
     5581 cttagcgcaa acagcgtggc gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat
     5641 gacgccgcag gcttataaca gtattcaata cgacgccgaa aaatcgctct ggcataacgt
     5701 tgagaaccgt caactggacg ctcagttctt ccatatggga atgggattcc gtcgccgcgt
     5761 tcgtatgttt tctgtagatc cagcaacaca tctggcgcgt gaaattcact ttcgcccgga
     5821 gttgttcaaa tacaacgatg caggtgttga taccaaacaa ttagaagggc aaagcgatct
     5881 cggctttgcc ggttttcgcg tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc
     5941 atttctcggc gcgagttatt tccgcgccgt tgatgataca tatcaatacg gtttgtcggc
     6001 ccgcggcctg gcgatcgaca cttacaccga cagtaaagaa gagttccccg actttaccgc
     6061 cttctggttt gatacggtaa aaccgggggc aactaccttt accgtttatg cgttgctcga
     6121 tagcgccagc attactggtg cctataagtt cactatccat tgtgagaaaa gtcaggtgat
     6181 tatggatgtg gaaaatcacc tgtatgcgcg caaagacatt aaacagctgg gcatttcgcc
     6241 gatgaccagt atgttcagct gcggtactaa tgaacgtcgg atgtgcgata caattcatcc
     6301 gcaaattcat gactctgatc gtctgtccat gtggcggggc aacggcgagt ggatttgccg
     6361 tccgctgaat aatccgcaaa aattgcagtt caatgcttac accgacaaca acccgaaagg
     6421 gtttggttta ttgcaactgg atcgtgactt ctcccattat caggacatta tgggctggta
     6481 taacaaacgc ccaagtctgt gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg
     6541 cctgatggaa atcccaacaa cgggcgaaac gctggataac attgtctgct tctggcagcc
     6601 agaaaaagct gtaaaagcag gtgatgagtt tgcattccag tatcgtctgt actggagtgc
     6661 gcaaccgcct gttcattgcc cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg
     6721 tttcccggaa ggttgggcgc caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc
     6781 cgtcgatttc gttggtggtg atctgaaagc tgccgcgcca aaaggcattg agccggtgat
     6841 tacgctttcc agtggggaag cgaagcaaat cgaaattctc tatattgaac ccatcgatgg
     6901 ttatcgtatt cagtttgact ggtatccgac ttcggactcc actgatccgg tcgatatgcg
     6961 gatgtatcta cgttgtcagg gggacgctat cagtgaaaca tggctgtatc agtatttccc
     7021 gccagcgccg gataaacgtc agtatgttga cgaccgcgtg atgagttaat cgttttttct
     7081 tcggcacctt cttcgggagg tgccgtctgg ttaaacacga tcccgctcgc atttttccct
     7141 aagttaaatg agtaatctga tggtgtgtat ttcagataca ccgtgtcagc cactaacagg
     7201 gagtgcgtat gtttccagaa taccgagatt taatatcccg tctgaaaaac gaaaatcctc
     7261 gctttatgtc cttgttcgat aaacacaata aacttgatca tgaaattgcc agaaaggaag
     7321 gttccgacgg tcgagggtac aatgcggaag tggtccgcat gaaaaaacaa aagctacagt
     7381 taaaagatga gatgctcaaa atcctgcagc aggagagcgt caaagaggtg taaactttcc
     7441 taagccgcca gccaggcggc ttttttaaca acagcatgga ttgactggag ataagatgac
     7501 tgaaacgata aaagtaagcg aatcacttga attacatgct gttgcggaaa gtcacgtcac
     7561 acctctttat cagttaatct gtaaaaataa aacctggtta cagcagtcgc taaactggcc
     7621 gcagttcgtt cagagtgaag aggacacgcg aaaaacggtg cagggtaatg tgatgttgca
     7681 tcaacgcggc tatgccaaaa tgttcatgat tttcaaagaa gatgaactta tcggcgttat
     7741 ctcgtttaat cgtattgaac cactgaataa aactgctgaa atcggctact ggctggacga
     7801 atctcatcag gggcagggga tcatttctca ggcgctgcag gcattgattc atcattacgc
     7861 tcagtctggt gaacttagac gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa
     7921 ccaggtcgct ttgcgcaatg gttttatcct tgaaggttgc ctgaaacagg ctgagttcct
     7981 gaatgatgcc tatgatgatg tgaacttata cgcgcgtatt atcgattcat aataaccctg
     8041 ccagcggcgt tcgcgtaatg cgctcctcgc cgttaatgac ttttggacca cggacatgaa
     8101 ccgtgtcgcc atcaaaggct tcaacgacgg cgtggtcggt taactccaca tggttttcaa
     8161 ttatcaccgc acctgtaata cggctctcac cttgtattac aacgtgctcg tcgagcagaa
     8221 tcggtccacc acgtaccacg gcatttccgc caatcaagac atgatgtttt aacacacaat
     8281 taccttccac aatggcatat tccgccacct gcgaactgta atgaatcgtc ggaatggcat
     8341 cttcttctat gccagctttc acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc
     8401 agacgttgtt ctcttcatta ccttcaacac tggcaaaatc aaaaacttcg gcgcgatgtt
     8461 caataaaagc ataccggacg acggcatcgc cataaatttg tgcctggtgg acaatacgcg
     8521 aggcgctaac ccttgcgcga tcataaattt ggagcaggag ttgatggtcg ggcgttaagc
     8581 cttgtgcggc gacgatcata gaatgttgat caattaaggc gtgaccaaat attctacatt
     8641 gcacgtaaac taatgagtca tgaatggtga cactgtcact tatatatgca ccctggctga
     8701 tttcactatt atcgatccag acgttatccg ttgcgtaaac ctctccccac agcacgctgg
     8761 tgccggtgat acgcgtatta ccggaaatca ccgttccgcc gaacgcaata gcattctggt
     8821 cgtaaatcca gcaatttcct tcctgtgcta acaccgtttc gcgatcgatc cagccgctat
     8881 cacatcgtta aaatcgctaa tggcgataat ctgccgtaat aacacacttt ttttagtgcc
     8941 atcttcctga taactaaagg ctcgctgttc ttcacttagg cgatatttgc gcatagcgtt
     9001 ttcccacagg tgacttacta taaccgtagc aaattctgcg gctctggcta tgctcgagaa
     9061 aattccataa aatgcatttc aaatatactt tataaattaa acaaaatgag taagaagatg
     9121 cagagcgata aagtgctcaa tttgccggca ggctactttg gtattgtgtt ggggacgata
     9181 gggatgggat ttgcctggcg ctatgccagc caggtttggc aggtcagcca ctggttaggg
     9241 gatgggctgg tgattctggc gatgagcatc tggggattat tgactagcgc atttattacc
     9301 cgactcatac gctttccgca tagcgtgctg gcggaagttc gccatccagt gctgagcagt
     9361 tttgttagtt tgtttccggc aacgacgatg ctggtggcga ttggttttgt tccgtggttt
     9421 cgcccactgg cggtgtgcat gttcagtttt ggtgtcgtgg ttcagttggc ttatgccgcc
     9481 tggcaaactg cgggattatg gcgcggatct caccctgaag aagctaccac gcctggactg
     9541 tatctgccga cagttgccaa caactttatc agcgcaatgg cctgtggtgc gttgggctac
     9601 accgacgccg gtctggtgtt tttaggcgca ggcgttttct catggctaag ccttgaaccg
     9661 gtgatcttgc agcgtctgcg cagttcggga gaattaccca cggcactgcg gacatcactc
     9721 ggcattcagc tcgctcctgc gctggtggcc tgtagtgcct ggctgagcgt caacggcggc
     9781 gagggtgaca cgctggcgaa aatgcttttc ggttatggac tgctgcaact gctgtttatg
     9841 ctacgtctga tgccatggta tctctcccag ccatttaatg cttcattctg gagtttctcg
     9901 ttcggcgtat ctgcactggc aaccaccggt ttgcatctgg ggagtggcag cgataatgga
     9961 tttttccata cgctggcggt gccgctgttt atctttacca attttattat tgcaatactg
    10021 ctcatccgta cttttgcgct tctgatgcag ggaaaattgt tagtcagaac cgagcgcgcc
    10081 gttttaatga aagcagagga caaagaatga tcattcgtga cgaaaactat tttactgata
    10141 aatatgaatt aacccgcaca cactctgaag tactggaagc ggtgaaagtg gttaaaccgg
    10201 gtaaaacgct ggatctgggc tgtggcaatg gtcgtaacag tctttacctg gcagccaatg
    10261 gttatgatgt tgacgcatgg gataaaaatg ccatgagtat cgccaacgtc gagcgcatta
    10321 aatccattga aaatctggat aatttacaca cccgggtcgt tgatctgaat aacctcacat
    10381 ttgatggaca gtacgatttt attctttcga ctgtggtgct gatgttcctt gaggctaaaa
    10441 ccatcccagg gttgattgcc aatatgc
//
LOCUS       DAJVPP010000123        10448 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVPP010000123 DAJVPP010000000
VERSION     DAJVPP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.123"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..305
                     /locus_tag="OQM34_003419"
     CDS             <1..305
                     /locus_tag="OQM34_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453734.1"
                     /translation="NLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITRR
                     MLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(508..861)
                     /locus_tag="OQM34_003420"
     CDS             complement(508..861)
                     /locus_tag="OQM34_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1453735.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(999..2645)
                     /gene="groL"
                     /locus_tag="OQM34_003421"
     CDS             complement(999..2645)
                     /gene="groL"
                     /locus_tag="OQM34_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1453736.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2689..2982)
                     /locus_tag="OQM34_003422"
     CDS             complement(2689..2982)
                     /locus_tag="OQM34_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1453737.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3258..4514
                     /gene="yjeH"
                     /locus_tag="OQM34_003423"
     CDS             3258..4514
                     /gene="yjeH"
                     /locus_tag="OQM34_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1453738.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(4530..5006)
                     /gene="fxsA"
                     /locus_tag="OQM34_003424"
     CDS             complement(4530..5006)
                     /gene="fxsA"
                     /locus_tag="OQM34_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1453739.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5343..6779
                     /gene="aspA"
                     /locus_tag="OQM34_003425"
     CDS             5343..6779
                     /gene="aspA"
                     /locus_tag="OQM34_003425"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1453740.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6897..8198
                     /gene="dcuA"
                     /locus_tag="OQM34_003426"
     CDS             6897..8198
                     /gene="dcuA"
                     /locus_tag="OQM34_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1453741.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8314..8652
                     /gene="cutA"
                     /locus_tag="OQM34_003427"
     CDS             8314..8652
                     /gene="cutA"
                     /locus_tag="OQM34_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1453742.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8628..10325
                     /gene="dsbD"
                     /locus_tag="OQM34_003428"
     CDS             8628..10325
                     /gene="dsbD"
                     /locus_tag="OQM34_003428"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1453743.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10362..>10448
                     /locus_tag="OQM34_003429"
     CDS             10362..>10448
                     /locus_tag="OQM34_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCS1453744.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAE"
BASE COUNT         2450 a         2745 c         2583 g         2670 t
ORIGIN      
        1 ataacttgtt cgaccattat ctggcggtgc tttaccgctt taaagatgaa agtggcaaag
       61 aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac gccgggtaaa gaaatagagg
      121 cgatcactcg ccgaatgctt gatttcagcc cacgactgaa aaaactcgcg ggtgtgccat
      181 gtcaggtcta tgttcgcacc gtagcggcga ataacgccca accgctaacc caggatcaat
      241 gcttacgcgc gctgcatcac ttacgtgtac aatccaccag caaaactgca ccacaggcga
      301 aataatctca tctttgctaa tgcgaggctg gagccatcca gcctgaaatg actgataacg
      361 tgatcgttgg tcatgccgga tgcggcgtaa acgccttatc cggcctacaa attctcaagc
      421 attcaatcag ttgcaatgcc tgctgtaggc ctgataagcg aagcgcatca ggcaataata
      481 cgtttgtcac ccagcctctg gatcacatca gttcgggcac ttataaacct gaccgataat
      541 ctggctatcc gtcgggacaa aactggacaa cattccctgc gacgggctgc tgatgccata
      601 aatcacgtta ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat
      661 agaaccgccc tcttctccgt gttgcccgga aagccagttg ctttgcttac ctgtcgcagt
      721 accaatcagc tggcactctg cgcctggctg ctcgtccaca atgcgtacac tctgaccggc
      781 agcactcaat tcgttgctgg agctacaacc cgccatcagt agcgcggcac cgacaatccc
      841 tgctaagtat tttacgtgca tgttattccc cataatcaat gagctggacg ccactcgcgt
      901 catccgtgtc tgaatcttat actagaaaga tgaccaaaag aaaaaccccc agacatttct
      961 gcccgggggt ttgtttattt ctgcgaagtg cagggcaatt acatcatgcc gcccatgcca
     1021 cccatgccgc ccataccgcc agcagcgcct aagtcagctg catcgttttt cggcaggtcg
     1081 gtaaccatgc attcggtggt gatcatcagg ccagccacag aagctgcgta ctgcagagca
     1141 gaacgggtta ctttggttgg gtccaggata cccatgtcga tcatgttgcc gtattcttcg
     1201 gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc aacaacagac
     1261 ggttcttcgc cgcagttcag gacgatctga cgcagcggag cttccattgc acgcagtgca
     1321 actttgatac ccacgttctg gtcttcgttc tgaccacgca ggtcagccag tttagacgct
     1381 acgcggatca gcgcaacacc accaccagca accacgcctt cttctaccgc agcacgggtc
     1441 gcgtgcaggg catcttcaac gcgtgctttt ttctctttca tttcaacttc ggtagcagca
     1501 cctactttga taactgcaac gccgcctgcc agtttcgcta cgcgctcctg cagtttttca
     1561 cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac acggccctgg
     1621 attgcagctt cttcacccac gccatcgatg atagtggtgg tgtctttgtt gatcacaaca
     1681 cgtttagcct gacccaggtc ttccagggtt gctttttcca gctccatacc gatctcttca
     1741 gagattacgg taccgccagt cagggttgcg atatcctgca gcatagcttt acgacgatcg
     1801 ccgaagcccg gagctttaac cgcagcaact ttcacgatgc cacgcatggt gttaacaacc
     1861 agagttgcta gcgcttcgcc ttcaacatct tcagcgatga tcagcagcgg tttgcctgct
     1921 ttggcaacag cttccagaac cggcagcatt tcgcggatgt tagagatttt cttgtcagcc
     1981 agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt gatgaagtaa
     2041 ggagacaggt agccacggtc gaactgcata ccttcaacca cgtccagttc gtcctgcaga
     2101 ccggtaccgt cttcaacggt gataacgcct tctttaccga ctttgtccat cgcttcagcg
     2161 atcagtttac ctacggtttc gtcggagtta gcggagatgg taccaacctg agcaatcgct
     2221 ttagaatcag agcacggtac ggacagcgct ttcagttctt caactgcagc ggtaaccgct
     2281 ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaacagc tttcagacct
     2341 tcagtgatga tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc tgcagcgtcg
     2401 ttcgctttgg aggcaacttc tttcaccatc tgcgcaccca tattttcgaa cttgtcttcc
     2461 agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc accgaaagat
     2521 ttatccagaa ctacgttacg gccttttgga ccgagggtaa ctttcactgc atctgccagt
     2581 acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac gtctttagct
     2641 gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgcggatt acgcttcaac
     2701 aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga tcttctcgga
     2761 tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacgt ccagcggctt
     2821 cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc cgcgggtgga
     2881 tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt caacttcttt
     2941 acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact ctcctttgag
     3001 aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc tggtgaccag
     3061 agaaatgggg atgaggcttc gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc
     3121 acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgcaatttct tcatcgccgt
     3181 cataagcgaa tctgattgtg ctaccatcga aaatctacgc agttgcccaa aatttgggcg
     3241 caatcggata tcaacccatg agtggactca aacaagaact ggggctggcc cagggcattg
     3301 gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc cgttcctgcg ttagctgcac
     3361 tggtagcagg caataacagc ctgtgggcgt ggcccgtttt gattatctta gtgttcccga
     3421 ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag cgcaggcggc gtcgcgcact
     3481 tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac cggctggctg tttttatcgg
     3541 tcattcccgt gggtttgcct gccgcactac aaattgccgc cggattcggc caagcaatgt
     3601 ttggctggca tagctggcaa ctgttgttgg cagaactcgg tacgctggcg ttggtgtggt
     3661 atatcggtac tctcggtgcc agttccagtg ctaatctaca aaccgttatt gccggactta
     3721 tcgtcgcact gattgtcgct atctggtggg cgggcgatat caaacctgcg agtatcccct
     3781 tccccgcgcc aggaaatatc gaacttaccg ggttatttgc tgcgttatca gtgatgttct
     3841 ggtgttttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa aatccagagc
     3901 gtgattttcc tcgtgctttg atgattggtc tgctgctggc aggattagtc tactggggct
     3961 gtacggtagt cgtcttacac ttcgacgcct atggtgaaca aatggcggcg gcagcatcgc
     4021 ttccaaaaat tgtagtacag ttgttcggtg taggagcgtt atggattgcc tgcgtaattg
     4081 gctatctggc ctgctttgcc agtcttaaca tttatataca gagcttcgcc cgcctggtct
     4141 ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct cgccatatcc
     4201 cgaataatgc cctcaatgcg gtgcttggct gctgcgtggt gagcacgttg gtaattcatg
     4261 ccttagagat caatctggac gctcttatta tttatgccaa tggcatcttt attatgattt
     4321 atctgttatg catgctggca ggctgtaaat tattgcaagg tcgttatcga ctactggcgg
     4381 tggttggcgg gctgttatgc gttctgttac tggcaatggt cggctggaaa agtctctatg
     4441 cgctgatcat gctggtgggg ttatggctgt ttctgccaaa acgaaaaacg ccggaaaatg
     4501 gcataaccac ataatccggc gtttcgacat taatcctggc gatcgtcttt atgatcaagg
     4561 cggtcgcggt catcatcctt tcgctgatac tcaccatcaa aagtattacc gccaccggtc
     4621 ccggcgctaa aaccgccgcc tggcatgcga gaaaagcgca aatgtggcat caacttcact
     4681 gtcagatgct tttgcaccgg cggcaataaa agcagaagac cgaggaagtc ggtgaaaaag
     4741 cccggtaata aaagcagcaa accggcaata atcagcgaaa cacttttaat catctccgcc
     4801 gcagggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt aaagccctgg
     4861 ttacgtacca gtgacatacc gataactgac gtgaatataa ccagcacgag ggtcagcaat
     4921 acccccaata catgggcaac ctgaataaag attgaaatct caatatagac ataaaggaaa
     4981 atggcaataa aaggtaacca gcgcaaaggt ttctcctgta atagcagccg gttaaacccg
     5041 gctacctgaa tgggttgcga atcgccttca gcttatattg tggtcattag caaaatttca
     5101 agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta
     5161 ataattaagg ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga aatcctcaag
     5221 cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg aaaaacattc
     5281 gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc
     5341 acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa gttccagctg
     5401 atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc agcaacaaca
     5461 aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcca
     5521 tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc attgccgcat
     5581 gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac gtctaccagg
     5641 gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat atcggtctgg
     5701 aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac catgttaaca
     5761 aatgtcagtc cactaacgac gcctacccga ccggtttccg tatcgcagtt tactcttccc
     5821 tgattaagct ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg
     5881 aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta ccgatgaccc
     5941 tcggtcagga attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa aacatccaac
     6001 gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt actggtctga
     6061 acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc actggcttcc
     6121 catgcgtacc ggctgaagac ctgatcgaag cgacctctga ctgcggcgct tatgttatgg
     6181 ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac gacctgcgct
     6241 tgctctcttc tggtccacgt gccggcctga acgagatcaa cctgccggaa ctgcaggcgg
     6301 gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat
     6361 gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca ggtcagctgc
     6421 agttgaacgt tatggaaccg gtcattggcc aggccatgtt cgaatccgtt cacattctga
     6481 ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct aacaaagaag
     6541 tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac ccgttcatcg
     6601 gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg
     6661 aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt ttctccgtac
     6721 agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc gaacagtaat
     6781 cgtacagggt agtacaaata aaaaaggcac gtcagatgac gtgccttttt tcttgtgagc
     6841 agtaacttaa aaataacaat ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc
     6901 tagttgtaga gctcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa
     6961 taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac
     7021 ccggtaacat cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg
     7081 ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc
     7141 gtaacccgaa atacatcacg atcctcgcac cgatcgtgac ctatttcctg actatctttg
     7201 ctggtactgg caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac
     7261 aaggcgttaa accttgccgt ccgctctcta ctgcagtggt atccgcgcag attgcgatca
     7321 ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtgatggaa ggtcatggca
     7381 tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga
     7441 tgtccttcct ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca
     7501 agcgtctgga agagggcttg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg
     7561 gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt atctatgcaa
     7621 tcatcaacag cccaagcatg ggtctggttg aaaaaccgct gatgaacacc accaacgcaa
     7681 tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg
     7741 acaacatcct caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg
     7801 gtgttgcgtg gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg
     7861 ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc tttgcttctg
     7921 ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga
     7981 acgtttcacc gctgaccgct gttgcttctt tcgctgcggt gtctggtctg ttcattctgc
     8041 cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtact acccgtatcg
     8101 gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gccctggccg
     8161 tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg atcaatcgcg gggcgttcac
     8221 gccccgcttt ctttcccgcc gactaacatc cttcccccgt ccgttgtata gtgacctctc
     8281 tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag ttcgaatacc
     8341 gcgtctgtcg tggtgctatg taccgcaccg gatgaagcga cagcccagga tttagccgcc
     8401 aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga tccccggcgc tacctctctc
     8461 tattactggg aaggtaagct ggagcaagaa tacgaagtgc agatgatttt aaaaactacc
     8521 gtatctcacc agcaggcact gctggaatgc ctgaagtctc atcatccata tcaaaccccg
     8581 gaacttctgg ttttacctgt tacacacgga gacacagatt acctctcatg gctcaacgca
     8641 tctttacgct gattctgcta ctttgcagca cttccgtttt tgccggatta ttcgacgcgc
     8701 cgggacgttc acaatttgtc cccgcggatc aagcctttgc ttttgatttt cagcaaaacc
     8761 aacatgacct taatctgacc tggcagatca aagacggtta ctacctctac cgtaaacaga
     8821 tccgcattac gccggaacac gcgaaaattg ccgacgagca gctgccgcaa ggcgtctggc
     8881 atgaagatga gttttacggc aaaagcgaga tttaccgcga tcggctgacg cttcccgtaa
     8941 ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc tgtgctgatg
     9001 ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg gtcgccaaca
     9061 acgcagcgtc acagcctgtg tctgtttcgc agcaagagca gcacaccgcg caattgccct
     9121 tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca tgcgtgctgc
     9181 caatgtaccc actgatttct ggcatcgtgc tgggtggtaa acagcggctc tccactgcca
     9241 gagcattgtt gctgaccttt atttatgtgc aggggatggc actgacttac acggcgctgg
     9301 gtctggtagt tgccgccgca gggttacagt tccaggcggc gctacagcac ccatacgtgc
     9361 tcattggcct cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc ttgtttactc
     9421 tacaactccc ctcttcgctg caaacacgtc tcacgttgat gagcaatcgc caacagggcg
     9481 gctcacctgg cggtgtgttt gttatggggg cgattgccgg actgatctgt tcaccatgca
     9541 ccaccgcacc gcttagcgcg attctgctgt atatcgccca aagcgggaac atgtggctgg
     9601 gcggcggcac gctttatctc tatgcgttgg gcatgggcct gccgctgatg ctaattaccg
     9661 tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc aaaaccgcgt
     9721 ttggttttgt gatcctcgca ctgccggtct tcctgctgga gcgagtgatt ggtgatgtat
     9781 ggggattacg cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc tttatcacca
     9841 gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat aatcctgctg gcagcggcat
     9901 tggttagcgt gcgcccactt caggattggg catttggtgc gacgcatacc gcgcaaactc
     9961 aaacgcatct caactttaca caaatcaaaa cggtagatga gttaaatcag gcgctcgttg
    10021 aagccaaagg caaaccggtg atgttagatc tttatgccga ctggtgcgtc gcctgtaaag
    10081 agtttgagaa atacaccttc agcgacccgc aggtgcaaaa agcgttagca gacacggtct
    10141 tacttcaggc caacgtcacg gccaacgacg cacaagatgt ggcgctgtta aagcatctta
    10201 atgtccttgg cctaccgaca attctctttt ttgacggaca aggccaggag catccacaag
    10261 cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc acatttgcgc gatcgccaac
    10321 cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt gaagatgtac
    10381 tgggagaagc cctgaaatta ttagaattac aagggattgc caacaccacg ctggagatgg
    10441 ttgctgaa
//
LOCUS       DAJVPP010000124        10388 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVPP010000124 DAJVPP010000000
VERSION     DAJVPP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.124"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..300)
                     /gene="intS"
                     /locus_tag="OQM34_003430"
     CDS             complement(<1..300)
                     /gene="intS"
                     /locus_tag="OQM34_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCS1453745.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEW"
     gene            complement(462..536)
                     /locus_tag="OQM34_003431"
     tRNA            complement(462..536)
                     /locus_tag="OQM34_003431"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(501..503),aa:Arg,seq:cct)
     gene            complement(610..1542)
                     /locus_tag="OQM34_003432"
     CDS             complement(610..1542)
                     /locus_tag="OQM34_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS1453746.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1836..2591
                     /gene="mlaA"
                     /locus_tag="OQM34_003433"
     CDS             1836..2591
                     /gene="mlaA"
                     /locus_tag="OQM34_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS1453747.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGVNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2773..3831)
                     /locus_tag="OQM34_003434"
     CDS             complement(2773..3831)
                     /locus_tag="OQM34_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453748.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4197..5537)
                     /gene="fadL"
                     /locus_tag="OQM34_003435"
     CDS             complement(4197..5537)
                     /gene="fadL"
                     /locus_tag="OQM34_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1453749.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            5909..6193
                     /locus_tag="OQM34_003436"
     CDS             5909..6193
                     /locus_tag="OQM34_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1453750.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6373..7683
                     /gene="fadI"
                     /locus_tag="OQM34_003437"
     CDS             6373..7683
                     /gene="fadI"
                     /locus_tag="OQM34_003437"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1453751.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7683..9827
                     /gene="fadJ"
                     /locus_tag="OQM34_003438"
     CDS             7683..9827
                     /gene="fadJ"
                     /locus_tag="OQM34_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS1453752.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10030..>10388
                     /gene="sixA"
                     /locus_tag="OQM34_003439"
     CDS             10030..>10388
                     /gene="sixA"
                     /locus_tag="OQM34_003439"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1453753.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAEL"
BASE COUNT         2566 a         2407 c         2618 g         2797 t
ORIGIN      
        1 ccattccttg taaatcgcac taaatgaatt gttgttagac gaagcctttt tcgcctttac
       61 cggatcgatg ccagccgaga tgtctttcct cgcagtccat gctttatccc ttgcctcctg
      121 caaagtcata agcggatatt ttccgacggt caggattttc tccttaccgt caatcttgta
      181 gcgaagctgc catacctttt tcccggatac agggacataa aggtacaggc cattaccatc
      241 gagaaggcgg tatggttttt ctttcggctt tgctgcttca atctgcttaa cggtgagcat
      301 gggtaaaaat ccggtgggta aaattatttt atccactttt tacccgtcat ggagtgcggc
      361 tgtcaacgat ctgacgcgaa ccatgacgaa ctgtgaatat acggaaggct tgatattcag
      421 gggattttgc ggactggtac ggatgggagc gaactgataa atggtgtccc ctgcaggaat
      481 cgaacctgca attagccctt aggaggggct cgttatatcc atttaactaa gaggacaatg
      541 cggcgagtat acccgctaat ggactgcggg gtaagtacgc tgccgctcga ttgcttaaac
      601 cctcgccatt tatgctgggt ttttatcatt tttcttaatg ttttccgcac gttctgcttt
      661 ttggcgtgct tctgctttac gcttgttgct catgtcgtta cgaatctgtg catgactcat
      721 taacgcgaag ataaaggtgc cgccgcagat gttccccgct aaagtaggta gtgcgaaggg
      781 ccagatgaaa tcgctccagt gcagcgtacc gttaaacacc agatagagga tttcaacaga
      841 accgaccacg atatgggtgg tgtcacccag ggcaataagc caggtcatca atataatcac
      901 cacaatcttt gccgcacctg ctgcaggaaa catccatacc atagtggcga tcagccagcc
      961 ggaaatgatc gcgttggcaa acatctcgct gggggtgttc ttcatcacat ccatgccgat
     1021 tttgacaaat gcatcgcgag tttcttcatt gaagataggc atatattcaa atgcccacgc
     1081 agcaatacca gtcccgagaa tattacccag cagcacgact ccccataacc gcataagtaa
     1141 gccaacgttg ctcattgtcg gtttttgcat gacgggtagt accgcagtca cggtgttttc
     1201 ggtaaataat tgctggcggg ccattatgac gataataaaa ccaaaggtat aaccgagatt
     1261 ctccagcaag aagctgcccg gcacaccttc cagttcggca tgaaatatcc cttttgccag
     1321 taacgaagcg cccatcgaca gacccgccgc aatggctgac cacagtagcg ccattgcgtc
     1381 gcgttctagc tctttttcac catcctggcg gatatgctca tgaattgcca tcgctcggga
     1441 ggggagtcgg tcttcatcta tttctatttt tttgccgcgc tctttttctt cgctctcaac
     1501 ttcaatttcg tcgctgtgtt gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt
     1561 agcacgtata gctaaagcgt agcggctttt ttgctcgcaa ctggcgggag ttactctgaa
     1621 aatgtagaaa aggctgcgtt tgcctttttc tgtttctata gaatcaagta gcctacaggg
     1681 cggcgattac caggctatga tcaaatcagc aaatcagggc gtctggacat cagttgacgt
     1741 gctgttacaa tcgcccacac ctaaacaggc ggatacggta tcgttccgtc atggatggca
     1801 aactgcataa gccataaaaa aacagggaga catttatgaa gcttcgcctg tcggcgcttg
     1861 ctctgggaac tacgcttctg gtggggtgtg cgagttccgg tacagatcag caagggcgtt
     1921 ctgacccgtt agaaggggtc aaccgcacca tgtacaactt caacttcaac gtattagacc
     1981 cgtatattgt tcgaccggtc gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta
     2041 atggtttgag caactttact ggcaaccttg aagaacctgc ggtgatggtt aactacttct
     2101 tgcagggcga cccttatcag gggatggtcc actttacccg ctttttcctg aacaccattt
     2161 tggggatggg cggctttatt gatgttgcag ggatggcgaa cccgaaactg caacggactg
     2221 aacctcaccg cttcggtagt acgcttggtc attatggcgt gggttatggg ccttacgttc
     2281 agttaccgtt ctacggtagc ttcacgctgc gtgatgacgg tggtgatatg gcggatgctc
     2341 tttacccggt tctttcctgg ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag
     2401 ggatcgaaac ccgcgctcag ctgctggatt ccgatggcct gctgcgtcag tcgtccgatc
     2461 cttatattat ggtgcgcgaa gcgtacttcc agcgtcatga tttcatcgct aatggcggcg
     2521 aactcaaacc gcaggaaaat ccgaacgcgc aagcgattca ggatgattta aaagatatcg
     2581 attctgaata agaaacaaat aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat
     2641 ccctcaatta ccacaagtaa aaggagataa accgacaatg acccggacgt aatgatgtaa
     2701 aaatactgat gtttgtcact gactatttcc gatattcagg cgttgaaaaa tcaaaaaact
     2761 ggaaaaataa ttttaaatat catcaaaatt aacacctagt gcagatagtt gattaatttc
     2821 attttgatat ttcgtctggc gattttgaag tcttttttca actttatctt cgagattctt
     2881 aaagtctttg tatttatttt caggatggtc aacgtcaact ttgttagcga ataaagtaga
     2941 aattatttct tttacattat cgcgtctaaa ttttgcatcg tccacttgaa aattatatct
     3001 tattgctcga cctaaacgtt taaaaacttc gttaggttct ctcagtcttt ccccgttgag
     3061 ctcgtttaca acctgatgat cggaaccagt tgcaaatgat tctgccatga tagctttact
     3121 gctttcaaac cacgctgaag cactcctggc gttttcacta caatattggg tgagactatc
     3181 accattaata cgaattcctc tttgaatgtg gtagtcgtcc tgatcgtagt aaccgagaga
     3241 taagtaatca tttccgtttt tatttttaat tacaatagcg agagagtcgc cttttttatc
     3301 tttaccgtac caggtaaata agtccgttgg ttcgcttttc ccgctctcat caaattccgt
     3361 taccgtatgt ctgagtttgt gtgatatctt atcgaagata aaaccaggat ctccatcata
     3421 tatttctttt aatgcgctga agatcatttc cttgttattg atatcatgag tcttctcaac
     3481 tatgttgtca ttaatgatag tacggacgag ttctttgtct ttatagttcg caggtaattc
     3541 tattggttct tggttgtcga taccaatagt aatatggtct gtatcattgg agccaatatc
     3601 aatataagta actgaggaat tattaatatt agatctataa ggatcgtcgt ttactcgtat
     3661 ggcgtttttt ataagctcaa gtagatgttt aatacaggtg cttattcctt ccttaccatt
     3721 aaaaaggttt ttgaactctt gtactaatcc attaatttca tcattgttat tttcattgat
     3781 ggattctggg taagctgcgc tggtattgtt gattgaaata gatggtagca tgattttatt
     3841 ccatattgat tgatttgtta caaatatata ttacctaatg aaatagacta tcaaaaaacg
     3901 ctcatttatt tagaacctat ctttaatttg agtgtataag cgcacaatag ccaggggggt
     3961 tgagtatcct ggaaatgcag aaaagtaaac tctttgagaa ggacgagcag aacgcttgaa
     4021 aatatgcatt tcccttcagg tttgtctgtg gatactactt actgatttga aagggcaagc
     4081 tgccatcagg ctgtttgcaa aactcaggag gcaagcaatg aggaaactac tttcggtgaa
     4141 gtgggggagt taaagtcacc tgcatagcag gtgactttat ccaggcgaac gcgttatcag
     4201 aacgcgtagt taaagttagt accgaacagc caggctttac cttcagactc gaactggtat
     4261 gggccttcgt taattttcac gctctgaccg tgcatataag aaacaccaac gtcgactgaa
     4321 gcatctttat taaatgcgta agtcgtacct gcactcagcc agaaacggtc ctggtccgga
     4381 atggagatag aacgattctg agccggaact gggctgtcat caaaggcgat accggtacgg
     4441 aaggtccagt tatcatcgta gtaataagtg gtacccaacg cgatgcggta agcatcttta
     4501 aagccttcat gtttctggaa cagcgtgtcg ccactggttg aggtcgcttt cagctgctgg
     4561 aactgactcc agctggtgta agccaggcta tagtgaatcg cccactgtgg atcaacacgg
     4621 ttataacctg acacttccca catttcaggc aggttcagcg tcagataacc cgattgcgtt
     4681 gcgccacctg tcgcggtagg aattggcaaa ccgtagttat taaacgcacg attaagatcg
     4741 ctgctgtagt tacctttgaa gtcaattttc acttcagaac ggtaggtcag tgcatagcgg
     4801 ttatttttat ccagttcata caggattccg gcgttccagc caaagcccca ctggttaccg
     4861 ttcagatgag cgattttggt attactgtcg ataccgttgg cggtagctgc caatgcttgc
     4921 ccttgaggag tttgcccggc aggagattgc ataatctgac cagcaaccaa ctgccccaga
     4981 tcgcctgcga aacgttcaat tttcgcgcga gcgtagatgg cgttgaaacc aagaccaaag
     5041 ctccatgcat tatttaagcg atacgcaccg cttaagttca ggttcatggt ttcaaggtca
     5101 gttgtacccc cgacagagcc gcctgcataa gtatcattaa actctgttgc cagaccatag
     5161 ttagaggtaa tagaagcgcc ccaaccaaat tggtcgttaa tcggtgcaac aaagtgcatg
     5221 ttcggaaccc atgccgtagg cgcgatgtta tcggctttca ggctacgacc agacggagac
     5281 gttccgctga tattcacatc cgggtcaata taaaccgcac ccgcagaaaa tgtcgggcgg
     5341 tcaaacatgg taatcaatgc ggggttacgg ctaacgttac ctgcatcatc ggcaattgcg
     5401 ccttcccctg aataagcccg gcccaggcca gaggaagaaa attcgtttaa ctgaaagcct
     5461 gccgaccagg cctgggtgga gataagtgcc actgcgactg cgagagcaga ctttgtaaac
     5521 agggttttct ggctcatgac cataacctca atgatttatt tttatacaaa ctatgttacg
     5581 tgctgtaaca ggagcgcgaa gtgtagggtc tgaggtacat cttagaaatc agaccagtgg
     5641 cgagagtata ggtcggacca gctagaatgt tgcaagtatg tttccatctt ttttcaaata
     5701 tgatctaaga aacgcgatcc agctggcaaa atttggaggt tgcgcactcg cggattcggg
     5761 tgaattttgt tttagatcat ttttaagtgt gatttcggtc acttatccga ttttgcagaa
     5821 ttaacaactg gagcagcact ttccggaagc gtaaaatatc cacaacgttt atctggcacc
     5881 ttgggtgcac atacagagga aatcaacgat gagtaaatgt agtgctgatg aaaccccggt
     5941 ttgctgctgt atggatgttg gcaccattat ggacaactcc gactgcaccg cgtcttacag
     6001 ccgcgtgttt gcaaaccgcg cagaagcaga acaaacgctg gcggcgctga ctgaaaaatc
     6061 ccgtagcgtg gaatccgaac cgtgcaaaat ctccccgact ttcactgaag agtctgacgg
     6121 tgtgcgcctg gatatcgatt tcacttttgc ctgcgaagct gaaatgctga tcttccagct
     6181 gggtctgcgt taattcctct cgggtacgta cgcccctgtc agttgctggc aggggcgttt
     6241 tcttttcttt cttctgtctt cactgtgtca tagctcccac tttccttttt ctccgcttgg
     6301 ctaaatgtaa aaaaatggtt aagactatga tcaggtcaga ccactttatt tattttttta
     6361 caggggagtg ttatgggtca ggttttacca ctggttaccc gccagggcga tcgtatcgcc
     6421 attgttagcg gtttacgtac gccttttgcc cgccaggcga cggcttttca tggcattccc
     6481 acggttgatt tagggaagat ggtggtaggc gaactgctgg cacgcagcga gatccccgct
     6541 gaagtgattg aacagctggt ctttggtcag gtcgtacaaa tgcctgaagc ccccaacatt
     6601 gcgcgtgaaa ttgttctcgg taccggaatg aatgtgcata ccgatgctta cagcgtcagc
     6661 cgcgcttgcg ctaccagttt ccaggcggtt gcaaacgtcg cagaaagcct gatggcggga
     6721 actatccgag cggggattgc cggtggggca gattcctctt ccgtattgcc aattggcgtc
     6781 agtaaaaaac tggcgcacgt gctggttgat gtcaacaaag ctcgaaccat gagccagcgc
     6841 ctgaaactct tctctcgcct gcgtttgcgc gacttaatgc ccgtaccgcc tgcggtagca
     6901 gaatattcta ctggcttgcg gatgggcgac accgccgaac aaatggcgaa aacctacggc
     6961 atcacccgag aacagcaaga tgcattagcg caccgttcgc atcagcgtgc cgctcaggca
     7021 tggtcagacg gaaaactcaa agaagaggtg atgactgcct ttatccctcc ttataaacaa
     7081 ccgcttgtcg aagacaacaa tattcgcggt aattcctcgc ttgccgatta cgcaaagctg
     7141 cgcccggcgt ttgatcgcaa acacggaacg gtaacggcgg caaacagtac gccgctgacc
     7201 gatggcgcgg cagcggtgat cctgatgact gaatcccggg cgaaagaatt agggctggtg
     7261 ccgctggggt atctgcgcag ctacgcattt actgcgattg atgtctggca ggacatgttg
     7321 ctcggtccag cctggtcaac accgctggcg ctggaacgtg ccggtttgac gatgagcgaa
     7381 ctgacattga tcgatatgca cgaagccttt gcagctcaga cactggcgaa tattcagttg
     7441 ctgggtagtg aacgttttgc tcgtgaagta ctggggcgtg cacatgccac tggcgaagtg
     7501 gacgatagca aatttaacgt gcttggcggt tcgattgctt acggacatcc cttcgcggcg
     7561 accggcgcgc ggatgattac ccagacattg catgaacttc gccgtcgcgg cggtggattt
     7621 ggtttagtta ccgcctgtgc tgccggtggg cttggcgcgg caatggttct ggaggcggaa
     7681 taatggaaat ggcatcagcg tttactctta atgttcgtct ggacaacatt gctatcatca
     7741 ccatcgacgt accggatgag aaaatgaata ccctgaaggc ggaatttgcc tcgcaggtgc
     7801 gcgccattat taagcaactc cgtgaaaaca aagagttgcg cggcgtggtg tttatctccg
     7861 cgaaaccgga caattttatc gccggagcag acatcaacat gattggcaac tgcaaaacgg
     7921 cgcaagaagc ggaagcactg gcgcggcagg gccaacagtt gatggcggag attcatgctt
     7981 tgcccattcc ggttatcgcg gctattcatg gcgcttgcct gggcggtggg ctggagctgg
     8041 cgctggcgtg ccacggtcgc gtttgtactg acgatcctaa aacggtgctc ggtttgcctg
     8101 aagtacaact tggattgtta cccggttcag gcggcaccca gcgtttaccg cgtctgatag
     8161 gcgtcagtac agcattagag atgattctca ccggaaaaca acttcgggcg aaacaggcat
     8221 taaagctggg gctggtggat gacgttgttc cgcactccat tttgctggaa gccgctgttg
     8281 agctggcgaa gaaggatcgc ccatcttccc gccctctacc tgtacgcgag cgtattctgg
     8341 cggggccgtt aggtcgtgcg ctgctgttca aaatggtcgg caagaaaaca gaacacaaaa
     8401 ctcaaggcaa ttatccggcg acagaacgca tcctggaggt tgttgaaacg ggattagcgc
     8461 agggcaccag cagcggctat gacgctgaag ctcgcgcctt tggcgaactg gcgatgacgc
     8521 cgcaatcgca agctttgcgt agtatctttt ttgccagtac ggacgtgaag aaagatcccg
     8581 gtagtgatgc accgcctgcg ccattaaaca gcgtgggaat tttaggtggt ggcttgatgg
     8641 gcggcggtat tgcttatgtc actgcttgta aagcggggct tccggtcaga attaaagata
     8701 tcaacccgca gggcataaat catgcgctga agtacagttg ggatcagctg gagggcaaag
     8761 ttcgccgtcg tcatctcaaa gccagcgaac gtgacaaaca gctggcatta atctccggaa
     8821 cgacggacta tcgcggcttt gcccatcgcg atctgattat tgaagcggtg tttgaaaatc
     8881 tcgaattaaa acaacagatg gtggcggaag tggagcaaaa ttgcgccgct cataccattt
     8941 ttgcttcgaa cacgtcatct ttaccgattg gtgatatcgc cgctcacgcc gcgcgacctg
     9001 agcaagttat tggcctgcat ttcttcagtc cggtggaaaa aatgccgctg gtagagatta
     9061 ttcctcatgc gggtacatcg gcgcaaacca tcgccactac ggtaaaactg gcgaaaaaac
     9121 agggcaaaac gcccattgtc gtgcgtgaca aagccgggtt ttacgtcaat cgcatcttag
     9181 cgccttacat taatgaagct atccgcatgc tgaccgaagg tgaacgggta gagcacattg
     9241 atgccgcgct agtgaaattt ggttttccgg taggcccaat ccaacttttg gatgaggtag
     9301 gaatcgacac cgggactaaa attattcctg tactggaagc cgcttatgga gaacgtttta
     9361 gcgcgcctgc aaatgttgtt tcttcaattt tgaacgacga tcgcaaaggc agaaaaaatg
     9421 gtcggggttt ctatctttat ggtcagaaag ggcgtaaaag caaaaaacag gtcgatcctg
     9481 ccatttaccc gctcattggc gcacaagggc aggggcgact ctccgcaccg caggttgctg
     9541 aacggtgtgt gatgttgatg ctgaatgaag cagtacgttg tgttgatgag caggttatcc
     9601 gtagcgtgcg tgacggggat attggtgcgg tatttggcat tggttttccg ccatttctcg
     9661 gtggaccgtt ccgctatatc gattctctcg gcgcgggcga agtggttgca ataatgcaac
     9721 gactggccac gcagtatggt tcccgtttta ccccttgcga gcgtttggtc gagatgggcg
     9781 cgcgtgggga aagtttttgg aaaacaactg caactgacct gcaataagaa ggtcaaagct
     9841 atatgaatcc gcgctgaatg gcggagtgtt ggtcaaaatg taaacgcata ttgactatac
     9901 ttacgccatt gaggtaaaaa acagcgtttc attcggtgaa tggataaggc acaatgccgg
     9961 ccaccgtttt ctttctctgg tttcagatga aagaaaacgg gcgaatctgg ttaacaaaag
    10021 cggtgcaata tgcaagtttt tatcatgcgt cacggcgacg cagccctcga ttccgccagt
    10081 gattcggttc gtcctctgac cactaatggt tgtgacgaat ctcgcctgat ggcgaactgg
    10141 ctgaaaggtc aaaaagtgga aatcgaacgc gttctggtga gcccgttcct gcgagccgag
    10201 caaacactgg aagaagtagg ggattgtctg aacctgcctt ccagtgcaga agttctgccg
    10261 gagttaacgc cctgcggcga tgttggtctg gtcagtgcct atttgcaggc gttgaccaat
    10321 gaaggtgttg cttcggtact ggtgatctct cacttaccgt tagtcgggta tctggttgcc
    10381 gagttatg
//
LOCUS       DAJVPP010000125        10370 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVPP010000125 DAJVPP010000000
VERSION     DAJVPP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.125"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..216)
                     /gene="istB"
                     /locus_tag="OQM34_003440"
     CDS             complement(<1..216)
                     /gene="istB"
                     /locus_tag="OQM34_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCS1453754.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAV"
     gene            complement(213..569)
                     /locus_tag="OQM34_003441"
                     /pseudo
     CDS             complement(213..569)
                     /locus_tag="OQM34_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            649..1842
                     /locus_tag="OQM34_003442"
     CDS             649..1842
                     /locus_tag="OQM34_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCS1453755.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1868..2545)
                     /gene="istA"
                     /locus_tag="OQM34_003443"
                     /pseudo
     CDS             complement(1868..2545)
                     /gene="istA"
                     /locus_tag="OQM34_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
     gene            complement(2632..2993)
                     /locus_tag="OQM34_003444"
                     /pseudo
     CDS             complement(2632..2993)
                     /locus_tag="OQM34_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3332..3886)
                     /locus_tag="OQM34_003445"
                     /pseudo
     CDS             complement(3332..3886)
                     /locus_tag="OQM34_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            4029..4151
                     /locus_tag="OQM34_003446"
                     /pseudo
     CDS             4029..4151
                     /locus_tag="OQM34_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(4413..4979)
                     /gene="ipgB2"
                     /locus_tag="OQM34_003447"
     CDS             complement(4413..4979)
                     /gene="ipgB2"
                     /locus_tag="OQM34_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCS1453756.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(5261..5575)
                     /locus_tag="OQM34_003448"
     CDS             complement(5261..5575)
                     /locus_tag="OQM34_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453757.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RL"
     gene            complement(5653..5763)
                     /locus_tag="OQM34_003449"
                     /pseudo
     CDS             complement(5653..5763)
                     /locus_tag="OQM34_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6087..6764)
                     /gene="ospD1"
                     /locus_tag="OQM34_003450"
     CDS             complement(6087..6764)
                     /gene="ospD1"
                     /locus_tag="OQM34_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCS1453758.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(7135..7272)
                     /locus_tag="OQM34_003451"
                     /pseudo
     CDS             complement(7135..7272)
                     /locus_tag="OQM34_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(7354..7652)
                     /locus_tag="OQM34_003452"
                     /pseudo
     CDS             complement(7354..7652)
                     /locus_tag="OQM34_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8241..8400)
                     /locus_tag="OQM34_003453"
                     /pseudo
     CDS             complement(8241..8400)
                     /locus_tag="OQM34_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8404..9633)
                     /locus_tag="OQM34_003454"
                     /pseudo
     CDS             complement(8404..9633)
                     /locus_tag="OQM34_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(9653..10000)
                     /gene="tnpB"
                     /locus_tag="OQM34_003455"
     CDS             complement(9653..10000)
                     /gene="tnpB"
                     /locus_tag="OQM34_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1453759.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLRLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(10000..>10370)
                     /locus_tag="OQM34_003456"
     CDS             complement(10000..>10370)
                     /locus_tag="OQM34_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1453760.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
BASE COUNT         2672 a         2426 c         2349 g         2923 t
ORIGIN      
        1 caccgccggg aaggctgcca ttcgggtata catcgcctgt ttacgttgat gacgtgccag
       61 tttttcttca tgaagcagat gctccaggaa gtccatataa ctccattcct ggtctactgc
      121 ctgttgtgac agcgcaggcg ctgcgcttat aaggctttcc agttgcaact gcccggcgag
      181 cgccatcagt cgttgatgtt gcagttccat catcacgcca ctcctctgca gaatgagtcg
      241 tagatggaga gtggatgatg ctgggggggg ttgtcgaagc tcaccagatt ttcactagga
      301 tgcacgtcat actctttttt ctccggaggc agtgccagca tggactgctg ctcttcgagc
      361 cagcgatcac agggacgggc ctggattgtt tcatgctttc gttggttagc aacatcgtgc
      421 agccagcgca gaccgtggcg gttggctgtt tcaacatcga cagtgctccc catcgggcgc
      481 aggcgagtca ttagtgggat gtaaaaactg ttacgggtgt actgcaccat ccgttccacc
      541 ttacctttag tctgtgccct gaagggctga ggaatcccca gaaaacgaga caaacatagc
      601 attatgtgat ctactgattt gtgccaacaa ttcaatgaga tcactgctat gcctgttcat
      661 aaagtatgtc agaatttctt ccgggatgcc ttagcaccat ttcatcagta ccgacaaaat
      721 gccctgatgg atgccacaat ggcgttaatc aacggagcat ctctgacgca gaccagtatc
      781 ggacgttttt tgccgggtaa tgcacaagtt aagaataaga tcaaacgtat tgatcgcctc
      841 atgggtaatg aggcgctcca tcgcgatatt ccaatgattt tcaggaatat cacttcaatg
      901 ctgacacggc agttatcttt atgtgtaata gccgtagact ggagtggcta tccctcccag
      961 gagcatcatg ttcttcgtgc cagccttctg tgtgacgggc gttccattcc gttattgagt
     1021 aaggttgttc cttctgaaaa acaaaataat ccattgatac aacatgattt ccttgattcc
     1081 cttgctcaat ccctgccccc cgatgccagg gttattattg tcacggatgc tggatttcag
     1141 agcgcatggt ttcaccatat tacttctctg ggctgggatt ttattggtcg gatcagaaat
     1201 aacgttcagt attgcctcga taacgcgcct gaaagatggt taaaagtgtc ggatagccct
     1261 gaatgtaaaa caccggaata tatgggggct ggaaggctgg ttaaagaaag gaaaaaaagc
     1321 atccgggggc atttttacac ctataagaaa tcggcaaaag ggcgaaaaaa gaaacgttcg
     1381 aaaggtcaga gtggtttaaa caaaacagac aaagaacaga gcaaatcagc aaaagaagcc
     1441 tggttgatct tcagcagtac aaatgacttc agagcgcgag aaatcatcaa gttatacagt
     1501 cggcgaatgc agatagagca aaactttcgt gatgaaaaga acgggcgctt cggatttggt
     1561 cttcgggcca gcaaaagtcg ttcaacagga agaattctgg ttctgagtct gctggcaacg
     1621 ttgagcacta tcgtaatgtg gttactcggt tatcacgctg aaaataaagg gttacacctg
     1681 aaatatcagg ctaacagcat taaatcccgg cgggttatct catatctgac gttagcgaag
     1741 aatgttcttc gacactcgcc acttatttta agacgaacag tactgagcac agttctaaac
     1801 catctgtcca gaacctaccg aaatatggtg ctggtttatt agcagtaatt ttgtggggat
     1861 ccctcagcct gaaggggcga cacagtcggg gagagaagcc catctccttg ccgaactgcc
     1921 acagcgaagg atggaaccgg tgctgaccgg tctgatatgc gtcacgttgc agaaccacag
     1981 ttttcatatt gtcatacaac acttcgcgcg gcacaccacc aaagaagcgg aacgcattac
     2041 gatggcaggt ctccagcgtg tcataacgca tattgtcagt gaattcgatg tacaacattc
     2101 ggctgtatcc gggaacagca acgaacacgt gaagcggtga gcgaccatta cgcatagtgc
     2161 cccagtcaac ctgcatctgt cgtccgggtt cagtttcgaa ccgaacggca ggctcctgct
     2221 cctgaggaac cgagagagaa cgaatgaatg ccctgagaat ggtcattccg ccacgatatc
     2281 cctggtctct gatctcgcga gcgattaccg ttgccgggat tttgtaagga tgagcatcgg
     2341 cgatgcgttg acgaatataa tcccggtatt catccaggag tgaagcaaca gcaggtcgcg
     2401 gcgtatattt tggcggctca gattttgcct gcaaataacg tttaacggta ttgcgggaga
     2461 gccccagttc tctggcaatc gcccggctac tcattccctg cttgtgcagg attttaattt
     2521 ccataactgt ctcaaaagtg accataagct ctcctgaatc aggagagcag attaccccct
     2581 ggatctgatt tcaggcgttg ggtgtggatc actattgcac cgttcgtgac agtgttgttg
     2641 cgccttgctg ccaacgaagc tccactgccc atcaagttca cagataaggg caacatcagc
     2701 gtgagcgact ggcgcagatg ttatccgccg gggcgggagc tttttaatgt ccgtatgacg
     2761 gtattgatgc cgattttcag cgtacggcca gtatcgcgaa ctccggcacc gttgaaagcc
     2821 atttcagtga tttggttttt aactcccggt ttacgggcct cgtaacagta agtgagctga
     2881 aacacccggt gacagtcacg gcaacggaat cgatcatgtc cttttgggtt ctgcccgtga
     2941 cggtaaacct gaacggactg gcaacggggg caatgaaagt taactgtgac catgaaagaa
     3001 cctaaaagcg ggtattataa cccctaattc aactaattgg aggcattgtc atttttaatc
     3061 tatggataca cttaaaaatc ctctcacctt acgttaaaaa ccttctcatg tttagagttt
     3121 tttattatgt ttatcataat gatatgacga tctgagtttt taaaatcaag aagaaaagat
     3181 aagtttttta aatgcacagt gcctgtggat aactttgcgt aaatccggag gacagatcac
     3241 tctgaagcaa gacaacagca tctgaaaaca atatgctcac gtttcaacca cagggcgtaa
     3301 gtgatcctac ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt
     3361 ccggactgag gccgccacac caactgtgcc gtcgccagcg attgtaatca cattcgatat
     3421 aattaaacac tgttgcccgc attatttccc ggctgataaa gtgttctccg tggagacatt
     3481 ccactttcag cgaatgaaag aagctttcca cgcaggcatt atcgtagcag cagccctttg
     3541 cgctcatact gccacgcaga ttatgccact tcagcagcgc ctgataatct gctgaacagt
     3601 actgacttcc tcggtccgag tgaacgataa cgttccgggg tctcttacgc cgccacagcg
     3661 ccatctgcag ggcatcgcag gccagttgcg ccgtcaggcg tggtgacatg gaccagccaa
     3721 taacggcacg tgaccacagg tcaatgacca cagccagata cagccagcct tcatctgtac
     3781 gtaagtacgt gatgtctccc gcccacttct ggttcggacc actggcgtaa aaatcctgct
     3841 ccagcagatt ttctgacaca ggcaggccat gtgcgcggta actgaccgta ttgctgggta
     3901 agatcaattt gctcactggt atttactacg tgcatcgaat gagtgatcaa gagccgtatg
     3961 acgcgagagt gtcacgtacg gttccgtaag cagccggaga gtgaaactct ctccggctga
     4021 ctcgactcgc aggttccgac agcggtgacg aacatgcagc ggtgttgttc tcgctgatcg
     4081 gcacatgccg tctgaacaat gtggagccag aaaaatggct gcgttacgtt attgaacata
     4141 tccaggactg accggcaaac cgggtacgcg atctgttgcc ttggaaagtt gatctgactt
     4201 ctcagtaaat atcaatatga ttctgacgag ccgcttacct ttgagcagag aaaggggaga
     4261 gggagcccac atccatgagc taggaaaata caaaaactgg ctatacaaca attcaatata
     4321 ttaggactta cttgcgagga gaaatatagc accgctcagg caattcctgg gtatttactt
     4381 ttcttagttt ctttactcgg gatgttctgt catcagaaag gcgattctaa atttgtaata
     4441 tagtcactca tattcccacg taatttcttt agatcaatat attgcacatt tttctcaaaa
     4501 aactgatcgg aaataagatt tgcaacattg cttgttgtat catttttaac gcttctattc
     4561 atcccttcac aaagttcgtc cattttcttt ttgaaataac tgcttcctga tacaagatga
     4621 tttatggaac tttgtgcagc attgatgtct agagaaatgc gataatcttt ctcaacctgt
     4681 ttaaatatct gtttacggag attatcgccg atttttcggc tgtgttctgc acaaaactta
     4741 tcaatacatt ggtttaccac ccgggatacg tagacttttt tttcatcatt tttccaatct
     4801 gtaatatttt caccgatttg ttctgaaaca gagctcaaaa tactagtatt tattttggtt
     4861 gatgatattt ttactatcct tttccccatt gtacagcgga taatctcatc cacctttcct
     4921 gaaaaatatc ttttgtgaga taaactgatt ccaaaattat taaaagatgt tccaagcatt
     4981 ataatcacct tgtcatgtaa aatagtaaca ttgtcattat tttagctgat tcaaacaatt
     5041 ctgtaaatgc tttgagttct ttactccctt ggctcaatga tggaccgtct ggaatcccag
     5101 agactaaact ctctgagaaa gagagtatga aaaaacttgg ggcagtccct aaatgaaggg
     5161 accaacagcg gtaaaataac cggcatcatt cataatgatg ccctgttagt tgtagttatg
     5221 aagatttacg tcattaaaat tgctgttcgt agtgtcagtc ctatagtctg gcatcagctg
     5281 aggatcgtag ccgatacatc actggcttca cgacattcgt gttgaggcta ttcatgaaaa
     5341 ctccacgctg aaaacgccgt tttgtataaa cggtcacggt atgcccggag ccactgttgc
     5401 tgatgaattc gataaaacct tggcgtttct tgaagctatc gttaatgcgg ataatgaaac
     5461 aacgattggt gagattcgct cttttgccga cgccctggac gctgtccggt tcaatcgcaa
     5521 caaaatcaac cggcagctga gcaaacccaa ccttgcctct ctggcgctgg agcatgaagt
     5581 gatttggctg ggacgtagcc gctgacagct tctataaaag tatgatccta cccacgtaat
     5641 atgaacacag gcttaagtga ggttctggtt ttcaaattgt tcaggactga gacctccaca
     5701 ccaactgtgc cgtcgtcagc gattgtaatc gcacttgata taattaaaca ctgtatattt
     5761 catttgtgga gtctccagtc tgagagggct ttatgcattc cgtattgggc actatgatgc
     5821 cagaaatctg caaggctcca caccttcctg tatttccctt cctctatcac agtgcacttt
     5881 acttaatagg gtgggatgtg ttcattacat ttattgtgtt ttttatgatt gtttttgcgt
     5941 ccagtgtcgt tcgtcatggg aaactggttc tatgggtagt atcgctcagt ctagttaact
     6001 gtttgtttct ttttagaaat taagcagatc acacaattcg gggagagatc cttctaagtg
     6061 caattctttg cagctattta gcacacttaa aacaactctt gtattttgtc agaaataata
     6121 ttaaacatgt cttgttcatt attgttaagt ctgtctgcta tttctgtaag ttttatccca
     6181 ctatgatggc atatatttat atatgccatc atagcctcga tattcccttt ccctgcggcc
     6241 agaaataatc ctggtaaccc ctcattatct cttacatcca atagtttaat cgtttctttt
     6301 ggtgataaat caaaagtttt aataaaatct ccataagctt gaatcgcagc ggaatgacct
     6361 tcttgaagag ctatgaacaa agcagattcc ccagcattat ttcgtgctgc aagaacttct
     6421 agtttttcct ctttgggtat ttcggctgcc aacagcgatt caccgtattt acgcgctgca
     6481 tcggcatttc catgccaaat caatgaaaac aaattatggc ggttatactc cttattattc
     6541 tttgtcggaa tggatatgtt ctgtattttg gccaattttc tgaaagaaac accactatat
     6601 cccccccctg atttttcttc attgatggcg tgggatatag cggtatttgt cacgttaatg
     6661 atattatttt tcattccttt attttcatta gcagtattgc tgcctattat taggtgcata
     6721 tttttgttgt ttgctggatg taatccatag ttatttattg acatttttgt tcatcctttt
     6781 tgctaaaaaa aacaataacc tatttttgta atgtaataga tattagtcta ttgctaatct
     6841 aaaaaaacca ttgccacatt gaatggctat attcaccaat aaattctttc gatttcgtat
     6901 attgacatat cgtagatata tcttatccct aagattcaat aatcgagtcc gtttaccatt
     6961 ctgtgcaagc gccttcactc agaagtgacc gtcaaggcga tcaccaaact tgataataaa
     7021 gcagctcatt gccatacgcc agtccttaag gggcgttgtc catttctgtg atgctgccta
     7081 gatttaactg ccagctccgg cttatctaca tctaaatatg tgagcctaat accgctacac
     7141 tttgtccaga ctatgttttt ctccgggaag tgggctaacc gtctccataa agtgacgaac
     7201 ttttttcaac agtggccata ttgaacggca ctgatgatta cgggttattg tctcatgaag
     7261 agccggccac atcactgaca gcagtgactt caggccttca gcaccagaca gcctgaacca
     7321 gttaatccat cgcccgacaa atttagtaat ctagggctgg tttttcttca gctgttgcgg
     7381 ttcgaaagtg ccattacgat cacgcagagt acggatttcc agaggttcat ctccagtagt
     7441 gataatcttc gttaagcaac cattgcggga attgctgcca gatctaagct gatttttctc
     7501 gcacccaaga tgatgagtca tattagcatt gagtacggtt tcattttcag catccaatca
     7561 aactgactga gatcttcagg atttttgaga cttttggcta gttcattagc taaagcccgc
     7621 aacttattag atgtcaactg tttttcattc ataaattcac cttcatttga tatcaaatta
     7681 aatagttcaa catcagagaa ttatacaaac ttatgtacag gctcctatga tcttcgaatc
     7741 ctgaagatta tagtgaaaat aagttttcac gttgaaatcc cacatagtta gtatatgata
     7801 aataatcatg gatgacctga ttaacctctt ggggtagtgc ttttatagaa gaataaatat
     7861 tctactaatt taatacatca cctaaaatag tattgttttt tatggtcggt tgaaggtcgt
     7921 ggtgatgatg ttagtccttt tgagaatatt ccagtgccag atcttactta taataaaggg
     7981 gcttggcagt ttggataaac tattgttctt attccactaa tacaaacttt ttgtaaagta
     8041 tattaaatat acttcatttc atactgaaat ccccggaagt attcaatcaa acaattacac
     8101 cataaaagga agagggggcc ctaattaagt tgtcaagcat gtttatgata cctgcagggt
     8161 ataaattttc tggctgcaag cagacacata taaaacgtct ttattgatcc tacccacata
     8221 atatggacac agtcgtaagt gaggctctgg ttttcaaatt gttccggact gagaccacca
     8281 caccaacggt gccgtcgcca gcgattgtaa tcgcactcga tataactaaa cactgttgcc
     8341 cgcactattt cccgatggat aatagtgttc tccgtggaga cattccattt tcagcgaatg
     8401 aattttcatc tgttgctgta tccagtcata cagtgactgc atcagtggcg cggctctggc
     8461 ttttcttgcc gccagacgct gttctgctga acatccccgg acttctgcct cgatggcata
     8521 cagttcaccg atacgctgca gggcttccgt ggtgatgtcg gttggcactc ttgcatgcac
     8581 atcgtggatt tttctccgga catgagccat acatgcggct tccgttattc tgccggattc
     8641 gtacaacgcc cggtaaccac cgtaagcatc ggcctgaagc actccgctgt aaccggccag
     8701 atgattctgt ggatggatac ctttccggtc cggtgagtac gcgaaccaga ccgccggagg
     8761 catttccgaa ccggcgttac ggtcatcacg aacgtagacc cacagccggg cagtccttgt
     8821 tttaccgctg cccggctcct ggaccggaac ggggatatca tcggcatgga ctttaccggg
     8881 catcagcaca tactggcgca ggatgtcata cagcggttcc agcagttcag caacagcacc
     8941 tgtccagcgc cccagtgtgg cacggctcag ctccacgccc tgacgacggt atatttctga
     9001 ctggcggtat aacggcagat agtctgcata ttttccagca acaacatggg ccagaagccc
     9061 cgctccggca taactgcgtg caatgggttt tgaaggcacc ggtgcctgca cgatatggtc
     9121 acaccggcaa caggccagtt tcggacgttg tgtttcgata accttaaagg cgctgctgat
     9181 aagctccagc tgctctgaca catcacatcc cagagaactg agttcgccac cacaggccgg
     9241 gcagcattcc tcttccggac ggatgacccg ggtttcacgg ggaagtgagg caggtaacgg
     9301 tttacgggct gaagactggc gcaggggaga tggcagtacc gggtcatatt gctcacccag
     9361 cgtttccgcc atttcttcct gaagtgcgct gattcgctcc tgtgcttcca gtatctgccg
     9421 ttcggttttt gcgcgaagtt tttctgagct tttaccgaac tgcatgcgct gcagtttcgc
     9481 aaccagcgcc ttcagccggt tgatttcgga agcataagcc gccacccgct gtgagagcag
     9541 gcggttgtat ttcgccatct ggcggatggt gtcctgttgc gtctgcaaca gtgcccgcag
     9601 gcgggcattc tcatgagcaa gtgaggtgtc catatcctca ctttacaacg ggttatatgc
     9661 ggatgccagc gcgttccgtt cgtttcgggt gtttccagtc gatgccctca agaagcatgg
     9721 ataactgggc cggagtaagg tgcaccttgc cgtcacgggt gaccggccag acgaagcggc
     9781 cccgctccag gcgtctggta aagaggcgca gtccgtcact gtcagcccac aacactttta
     9841 tctggtcacc ccggcgtccg cggaagatga acagatgccc ggagaacggg tcatccttca
     9901 ggacgttctg aacttttgag gccaggccgt taaagccatt tcgcatatcg gtgatacctg
     9961 caaccagcca gatacgcgaa cctgcaggga aagatatcat cagtggctgc tcccttttat
    10021 ttcgcggata agtatctgta ataacgccgg cgtcagttta cctttaagcc tgagagttcc
    10081 ggccggcaga accagctcac agcacagact gtcggacggt gtatttatct gctctggttc
    10141 ctgtgccgga gccgggattt tattatccgg ctccggcgtt aacgtcacgg gaagcagtgc
    10201 aggcatattt tttccggaag gcagcaggcc acctttccgg tattgatggc gccagttgaa
    10261 gagcaggtta tcgttgattc cgttttcacg ggcgatctgc gccacacagg ctccgggctg
    10321 cagtgactgc tccactaaag cgattttaaa ttcataagga aaattaggcc
//
LOCUS       DAJVPP010000126        10294 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVPP010000126 DAJVPP010000000
VERSION     DAJVPP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.126"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..401
                     /locus_tag="OQM34_003457"
                     /pseudo
     CDS             1..401
                     /locus_tag="OQM34_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="OQM34_003458"
     CDS             484..741
                     /locus_tag="OQM34_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453761.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OQM34_003459"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OQM34_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCS1453762.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OQM34_003460"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OQM34_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCS1453763.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OQM34_003461"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OQM34_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCS1453764.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OQM34_003462"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OQM34_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCS1453765.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OQM34_003463"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OQM34_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCS1453766.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OQM34_003464"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OQM34_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCS1453767.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OQM34_003465"
     CDS             complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OQM34_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCS1453768.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9142..9324
                     /locus_tag="OQM34_003466"
                     /pseudo
     CDS             9142..9324
                     /locus_tag="OQM34_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9318..9422
                     /locus_tag="OQM34_003467"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="OQM34_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="OQM34_003468"
     CDS             9762..10019
                     /locus_tag="OQM34_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCS1453769.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2401 a         2735 c         2822 g         2336 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaatgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggc tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agca
//
LOCUS       DAJVPP010000127        10249 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVPP010000127 DAJVPP010000000
VERSION     DAJVPP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.127"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            11..1465
                     /gene="amn"
                     /locus_tag="OQM34_003469"
     CDS             11..1465
                     /gene="amn"
                     /locus_tag="OQM34_003469"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS1453770.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            1808..2524
                     /locus_tag="OQM34_003470"
     CDS             1808..2524
                     /locus_tag="OQM34_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1453771.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(2980..3055)
                     /locus_tag="OQM34_003471"
     tRNA            complement(2980..3055)
                     /locus_tag="OQM34_003471"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3020..3022),aa:Asn,seq:gtt)
     gene            complement(3156..4783)
                     /gene="yeeO"
                     /locus_tag="OQM34_003472"
                     /pseudo
     CDS             complement(3156..4783)
                     /gene="yeeO"
                     /locus_tag="OQM34_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4788..4863
                     /locus_tag="OQM34_003473"
     tRNA            4788..4863
                     /locus_tag="OQM34_003473"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4821..4823,aa:Asn,seq:gtt)
     gene            complement(4901..5851)
                     /gene="cbl"
                     /locus_tag="OQM34_003474"
     CDS             complement(4901..5851)
                     /gene="cbl"
                     /locus_tag="OQM34_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCS1453772.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(5953..6870)
                     /gene="nac"
                     /locus_tag="OQM34_003475"
     CDS             complement(5953..6870)
                     /gene="nac"
                     /locus_tag="OQM34_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCS1453773.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7198..7273
                     /locus_tag="OQM34_003476"
     tRNA            7198..7273
                     /locus_tag="OQM34_003476"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7231..7233,aa:Asn,seq:gtt)
     gene            complement(7329..8264)
                     /gene="ldtA"
                     /locus_tag="OQM34_003477"
     CDS             complement(7329..8264)
                     /gene="ldtA"
                     /locus_tag="OQM34_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1453774.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8326..9405)
                     /gene="cobT"
                     /locus_tag="OQM34_003478"
     CDS             complement(8326..9405)
                     /gene="cobT"
                     /locus_tag="OQM34_003478"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1453775.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9417..10160)
                     /gene="cobS"
                     /locus_tag="OQM34_003479"
     CDS             complement(9417..10160)
                     /gene="cobS"
                     /locus_tag="OQM34_003479"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1453776.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10157..>10249)
                     /locus_tag="OQM34_003480"
     CDS             complement(10157..>10249)
                     /locus_tag="OQM34_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCS1453777.1"
                     /translation="RDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2877 a         2608 c         2214 g         2550 t
ORIGIN      
        1 ggagacacac atgaataata agggctccgg tctgacccca gctcaggcac tggataaact
       61 cgacgcgctg tatgagcaat ctgtagttgc tttacgcaac gccattggca aatatattac
      121 aattggcgaa ttacctgatg aaaacgcccg taaacaaggt ctttttgtct atccatcact
      181 gaccgtaacc tgggacggta gcacaacaaa tccccccaaa acgcgcgcat ttggtcgttt
      241 tactcacgca ggcagctaca ccaccacgat tactcgccct actctctttc gttcgtatct
      301 gaatgagcaa ctaacgttgc tttatcagga ttatggtgcg catatctctg tgcaaccctc
      361 gcaacatgaa atcccttatc cttatgtcat cgatggctct gaattgacac ttgatcgctc
      421 aatgagcgct gatttaactc gctacttccc gacaacagaa ctggcgcaaa tcggcgatga
      481 aactgcagac ggcatttatc atccaacgga attctccccg ctatcgcatt ttgatgcgcg
      541 ccgcgtcgat ttttccctcg cacggttgcg ccattatacc ggtacgccag tagaacattt
      601 tcaaccgttc gtcttgttta ccaactacac acgttatgtg gatgaattcg ttcgttgggg
      661 atgcagccag atcctcgatc ctgatagtcc ctacattgcc ctttcttgtg ctggcgggaa
      721 ctggatcact gccgaaaccg aagcgccaga agaagccatt tccgaccttg catggaaaaa
      781 acaccagatg ccagcatggc atttaattac cgctgatggt cagggtatta ctctggtgaa
      841 tattggcgtg ggaccgtcca atgctaaaac catctgcgat catctggcag tgctacgccc
      901 ggatatctgg ttgatgattg gtcactgtgg cggattacgt gaaagccagg ccattggaga
      961 ttatgtcctt gcacacgctt atttacgcga tgaccacgtt cttgatgcgg ttctgccgcc
     1021 cgatatacct attccgagca ttgctgaagt gcaacgtgcg ctttatgacg ctaccaagtt
     1081 agtcagcggc aggcctggtg aggaagtcaa acagcggctg cgtacaggta ctgtggtaac
     1141 cacagatgac aggaactggg agttacgtta ctcagcttct gcacttcgtt ttaacttaag
     1201 ccgggccgta gcaattgata tggaaagtgc aaccattgcc gcgcaaggat atcgtttccg
     1261 cgtgccatac gggacactac tgtgtgtttc agataaaccg ttacatggtg agattaagct
     1321 tcccggccag gctaaccgtt tttatgaagg cgctatttcc gaacacctgc aaattggcat
     1381 tcgggcgatc gatttgctgc gcgcagaagg cgaccgactg cattcacgta aattacgaac
     1441 ctttaatgag ccgccgttcc gataataagt tatgaacctt cttatcagaa atagatgtga
     1501 aggattaagt aagacatctg gcaaatagcc tgcaataaac gcgggctgct tgcttccttg
     1561 ggcagatgcg agttttatta tcgtcttaat gatttccaca tattaaaagc aagtatgctt
     1621 tcaaaacaca attataaaaa atcccgccaa caacattagt ttttataaaa ttaaatataa
     1681 gattatggct ttagaatatt tttatttcta atagacgaga tttttcctgt tatgatataa
     1741 tatgccgaat taacacatgt taacgattta ccagtaatgt aaataaattt tcgaggagat
     1801 cattccagtg ggacgtaaat gggccaatat tgttgctaaa aaaacggcta aagacggtgc
     1861 aacgtctaaa atttatgcaa aattcggtgt agaaatctat gctgctgcca aacaaggtga
     1921 acccgatcca gaattaaaca catctttaaa attcgttatt gaacgtgcaa agcaggcaca
     1981 agttccaaag cacgttattg ataaagcaat tgataaagcc aaaggcggcg gagatgaaac
     2041 gttcgtacag ggacgttatg aaggctttgg tcctaatggc tcaatgatta ttgctgagac
     2101 attgacttca aatgttaacc gtacgattgc taacgttcgc acaattttca ataaaaaagg
     2161 cggcaatatc ggagcggcag gttctgtcag ctatatgttt gacaatacgg gtgtgattgt
     2221 atttaaagga acagaccctg accatatttt tgaaattttg cttgaagctg aagttgatgt
     2281 tcgtgatgta actgaagaag aaggtaatat tgttatttat actgaacctg ctgaccttca
     2341 taaaggaatc gcggcgctaa aagcagctgg aatcactgag ttctcaacaa cagaattaga
     2401 aatgattgct caatctgaag ttgagctttc cccagaagat ttagaaatct ttgaagggct
     2461 tgttgatgcc cttgaagatg acgacgatgt acaaaaagtt tatcataacg tcgcaaatct
     2521 ctaattatat tttaaagaaa tctgtcttta tggcagattt ctttaatctc atataattct
     2581 tataaaaaat ataatattta actcgtcata ttgcttataa aaaatccccg tccccagaga
     2641 aataatattt attaaaatgg caggtcttct ttttctgatt accgaagaca aagtggcaat
     2701 tacgtatagt ttcccgataa agacgcgaca gctacgtccc gcataaggca tttttctctt
     2761 tatctttgta cggtacttca tggaacagaa tttttgacct tgtgaatcgt gatatctgtt
     2821 ggggaggggc aatttgctca ctgaagcgtg agactcgatt aagcgcacga aacacagaaa
     2881 tcaaaaaacc cggccacttt tttacaaagt ggccgggtaa aaataatttt tattttttaa
     2941 ctgtcctgag tctcatagag atgtctcaaa actaaaattt ggctcctctg actggactcg
     3001 aaccagtgac atacggatta acagtccgcc gttctaccga ctgaactaca gaggaatcgt
     3061 gagaacgagg cgaatattag cgatgcccac ccacaatgtc aaagcctgtt ttttaaattt
     3121 gaaatcgttt gctgaaataa tctgcatttt gtcgtttatt ccgacacaac tggctttttt
     3181 tcacactttt gcggctcgga tcgagggtat ttccacagcc aacgtccagt aaccattcgc
     3241 cagtaaaaca gcacggcccg cacagcccag tcggcaaaca ttcccatcca gacaccaacc
     3301 acaccccagc caagcatgat tccctgcaca taaccgacta caacccggca accccacatg
     3361 ctcaacatag aaacccacat ggcgtaacgg gcatcacgag cacccttaaa tccagcgggt
     3421 agcacccatg aggcggacca aataggcata aataaagcat ttagctaaat cagaatcaca
     3481 acgacgtgtt taacctgtgg atcctgggtg taaaacgatg ccataacccc ggcaaaggga
     3541 gccgttagcc aggcgatggc cgttaatcca agagtggaca gccagaacac atgccgcaac
     3601 tgaatctctg cttgcgctat ctgccctacc cccaaccttc ggcctgtaat gatcgtagaa
     3661 gcagaaccga gcgcacttcc gggtaagttg ataagagccg caattgagaa cgcgataaaa
     3721 tttccggcaa taacgctggt ccccatcccg gcaacgaaca tttgggttaa taaccgacca
     3781 ctggtaaata acactgattc gacactcgct ggaataccaa tccccatgac ttcccagata
     3841 atgctaaaat tcagcggttt aaaatagctc tttaacgaaa tccttagcgc aggattaaaa
     3901 ccaatcgcca gcacccacaa aattgcaact gcgccaatat aacgagaaat ggttaaaccc
     3961 aacccagccc cgaccaggag aaaaggccgt aaatcaatat gccgctaata ataatattaa
     4021 gaatattcag gctaccgtta atcagtagcg gtattttcgt attccctgca ccacgaagtg
     4081 ccccgctacc aataagagtg atggcagcgg ctggataact gagtacggtc agctccagat
     4141 aagtcaacgc cagtgcttta acttctgtcg tggcatcacc cgcgacgaaa tcaataattt
     4201 gttcgccaaa atgatgaatt agcgttgcca acagtacggc aaacaacgtc atgatcacca
     4261 atgactgccg cgtcgccacc ctcgctcgtc gtcgatcccg cttaccgaga ctaaatgcta
     4321 caacgacagt agtaccaaga tcgatagctg caaaaaaagc cataatgacc atattgaagc
     4381 tgtccgccaa ccccacgccg gccatcgcat cttttcccag ccaactgacc agaaaagtgc
     4441 tcaggacccc catcaacagg acacaggcgt tctccatgaa gataggaaca gcaagcgggg
     4501 ttatctcgcg ctagaacaac actttgtagc tcttgcgttt agagtgccag cgagtgccgt
     4561 gaacaacctg gcgtaaagca gaggagatat tcaaagccga ccttaattgc agaaagtgaa
     4621 accacatttc aaatgatgag ggagaatcag caaagctgca aagattttcg ccaacaaatt
     4681 gtctgcaaat gcaacaaact gttgatagaa acggcaaaca gttgggaaat ttaaaaatcg
     4741 ggtttgacaa aatatttttc gccgttaaga tgtgcctcaa cagcgattcc tctgtagttc
     4801 agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag
     4861 ccaaatttct gaaaagcccg cttttatagc gggctttttg ctatatctga taatcaattt
     4921 cctcttcact gttttccatc acctggcgct tgatatcctc aactgacagt cctgcattac
     4981 aaagttccag aaagcgccaa acatagttac gctgaagttg tcctcgcttc agtcccaacc
     5041 agacagtatt agcatcgaaa agatgccgcg tatccaggcg gattaaattc tcttcctctt
     5101 gttcgccgct ggattgttcg gcaactaatc cgatcccaag cccaagagca acataggttt
     5161 taatgacatc agaatcctgt gcacttaata caatatctgc cggcaaacct ttgcgggtaa
     5221 atgcgtcatc agtgcgtgag cgccccgtaa ttccctgtcg gtaagtaatt aacggccact
     5281 tcgctattga ttccagcgtc aatggtgtaa tttgcgttaa gggatgatcg agtggaacaa
     5341 gcaaactatg gtgccaacaa aaccacggga aggcgacgag ctgcgggtca ttactcaaac
     5401 gctcgctggc gataccaata tcagcttcgc cattttgcaa caacgtcgca atttcctgtg
     5461 gcgaaccctg gattagctcg agccgaacct ccgggaaaag ttcgcgaaaa gctttaatga
     5521 cctctggcaa gctataacgt gcctgagtat gcgtcgttgc aatagtgaga acaccagacg
     5581 tatcgttggt aaacaggtct gcaagccgac gaacattact ggcttcattc agaatacgtt
     5641 ctgcaatgac cagtaatgct ttgcccggtt cagtcatccc cagcagtcgc ttacctcgtc
     5701 gaacaaatat ttcgatgcca aattcatcct ccagttcccg aatatgacgg ctaacgcctg
     5761 actgtgaggt aaaaagcata ttcgcaacct ctgtcaggtt gtaatcctga cgtgcagcct
     5821 cgcggattat ctttagttgt tggaaattca cggtaaactc cgggcagttc agatatcccg
     5881 ttattgttaa agtctaatgc ccggcataac aaataataaa aacccgcatc ttattccatc
     5941 ccgatataac gcttagctca ccaattgcca ctgccttttt tccatcactg gagaactaat
     6001 cactgacatt aacaactctt tcactgcctg tgcctgtggt gacaagtttg ctctggcggg
     6061 taaatttaag gacaaagaga gactcattga aggagtggta atgcgtgaca tccacccatt
     6121 tactgcgcca cataacgaac gcgcagccga ttcaggtaat actgcaacgc ccatgccgct
     6181 ggcaatcgct gcggtaagcg tggcaataga ctcaatttca ccaatgactt ttgccgtgag
     6241 ccgccgtagg gaaaaagcct catcaacacg aagtctaaca gcgctgtaat cactggggag
     6301 aaaaaggttc atttgcgcaa tagcattaac atcaacgttt tgccccgggc aatcttgagt
     6361 tcctaccagg aaaagatctt ctttcagcaa agcctgactg gataccccag ccacagggga
     6421 atgctcataa atcaccgcca tatcgagttg gtgatttatc agtttttcgt taagcactgc
     6481 accactattt tcatgaagat agataacgac ctccgggaat tcagcgcgaa ccgcctgtaa
     6541 taagggcatg gtgatggatg acgcagcggt tcctggcgca aagccaatcg agacttgccc
     6601 cgataatgac tgaccaacgt tatgcaccgc cagttgggcc tgctcacact gacgtaaaat
     6661 ggcccgtgca tgggtataga gaatttttcc ggcgtctgtt ggcgtaacgc cccgttttgt
     6721 gcgaatcaaa agctgttgat ttaactcacc ttccagtatg gcaacctgct ggctgagcgc
     6781 tggttgtgcg atatgcaata cttcagcagc ctgggtcagg ctaccaatat ctacaatttt
     6841 tacgaagtat ttcaggcgtc tgaagttcat gttgcctccg gtttttaaga atcggcccaa
     6901 gtgccgccat tacttacaac cagtatgcaa gatgcctgcc agttctattt tggcgttgat
     6961 gcgcaagcta accaactgtc aaataagaga ttatgataga ttcgtcattg gcccttttta
     7021 tcagcagtcg cattcacctg ccctataaaa ggagggtatg caccacgatg gttcattacc
     7081 caataagatt gaaagctcac cactttgttg aaattggcag caaacaaaca aaaaaaatgc
     7141 atttcaccct ttgacatcac catgcactgc cattaatatg cgccccgttc acacgattcc
     7201 tctgtagttc agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca
     7261 gtcagaggag ccaaattcaa aaaagcctgc tttcgagcag gctttttact ttctaattac
     7321 caacgctctt aaaacatctg tcttgaacca gaactaattt gcacaggcat tcccgatcga
     7381 tgttgcaacg cagcatttgc gcgatttaca tcaacttctt gcccgttgat aaacgcccgc
     7441 aaagatgggg ttaccggcaa tggcactttt cggtcagact catattctgc acgattgcgc
     7501 gacagcggct catgaacttc cagccacttt gaaccatccg gttccgttgt gtatttcact
     7561 ggctgatcaa taatttgtac acgagtgcct acaggaacat tatcaaacag atatttgata
     7621 tcgtcattac gcagacgaat acagccctga cttacacgga gcccaatacc aaaattggca
     7681 ttggtaccgt ggatggcata caacctgcca atataaatcg cgtacagccc catggggtta
     7741 tcgggccccg caggaacaaa tgcgggcaaa ctctcccctc gtttcgcata ttcgcgccgg
     7801 gtgttcggcg ttggcgtcca ggttggtgct tcttgtttac gttcaacggt agtcacccag
     7861 ttacgcgggg tttctcgccc agcctggccg ataccaatag gaaagacttc cactgtatta
     7921 ctgtctggcg gatagtaata aagacgcatt tcagcgacgt taaccacaat ccctttacga
     7981 acagtggcgg gcaaaatcag ttgctgcgga atggtgagtt gtgagccagg cttcggcaaa
     8041 aaaacatcag cgcccggatt cgcttccagc atattactta acccttgccc gtattgtgcg
     8101 gcaaaagtct ccagcggctg agtattgtga tcaggaacag ttacagtaag cgactgcccc
     8161 actaaacggc taccctctgg aggtaatgga taagttaccg ccaggctatt ttggctggca
     8221 aaaagcagag caaatgagca aagaatattt acacgacgca tcatgtccct ttcctaagtc
     8281 gcgaatacta tccgataagt atagctttta tcagactttt cgtttttagc tgttcaaatc
     8341 agaagtcgta ttccccggta gaacaatatt actggcagca agttcgccca tgttgttgta
     8401 tatcgcacaa gcagcttcga tgatggacat cgccagagct gcaccactcc cctcacctaa
     8461 acgcatatcc atattgagat aaggctccag ccccaaatgc gagagcgcta tacgcgcgcc
     8521 tttttctgcc gacaagtgag aaggaatgag atacggtttg attgcaggag acatctggca
     8581 ggctgcgagc gcagcagcat aagaaagaaa tccatccagc aacacgggta aaccgcagga
     8641 agcagcacct aacatcactc cagctattcc gaccaaatca aatccaccca cttttgccag
     8701 gacatcaaca ccatcctgag gatttggttg attcaacgta atcgcccgac gcacaacatc
     8761 aattttatta gccagtttat ctgtcggcag gtttgcgcca atcccaacca cttcttcagg
     8821 atcccggcca gtgattgtgc tgactattgc cgctgccggt gtcgtgtttg ccatccccag
     8881 ttcacctaca ccaaacagcg tgacaccgtt tttcgccagc tcccgcgtat aacatatgac
     8941 gtccaaaagc aacttttcag cctgacggcg actcattgcc ggagctgaag caatattgcc
     9001 gctacctcgt gcgacacgca tgttgataag cccggggata ggctcagcag tatcaatacc
     9061 aacatcaatt acgtggacgt tagcgcccgc ttgtgctgcc agcacacaca cgccggttgt
     9121 tccacgggtc atattttcag cctgtatggc tgtcacttct tttggagaaa tagcgacccc
     9181 ttcctcccag acgccgtgat cggcacacat aaccagtacc gcttttttgc ccacatgcgg
     9241 tatgccattc acccccggca ttcctgccag ttgtatggca agcgcctcca gctttcctag
     9301 gctaccaaca ggtttgaata acccgtcaac atgccgttgt gcacgcgaca tagcggcaga
     9361 attgatggct gggatcgtat tcagtaaatc ggcaattatt tgcatgtcac gtttctttat
     9421 agcagtgcca gcaggaagac cagttcacca agctctatgg ctgctcccag cgtatcgccc
     9481 gtttgcccgc ccagcgtacg tttgagaagc tgaccgagga tgaaaatcgc caccatcgtc
     9541 accaccatag cagccacacc atgcataccc agcaataata cggcagcaaa aatagcggct
     9601 aagccgagtg tgacgcaggt ttgtcgccca tcaattttgc caatgaacac attgccaagc
     9661 ccttcttccc gcgcataacg atgacgatac atcaataatg cggcagtccc gcggctgacc
     9721 gcacatgctg ccgccagcga tgcaaggatc ggttcgccac gcagagccaa ctcgcttaac
     9781 accagaatct ttgccagtac cacaaaaatc aatgccagac cgccgtgggt gcctaaacgg
     9841 ctatcacgca tgatttccaa catgcgatcg cgactacgtg cagaaaatac gccatcacag
     9901 gtatcagcga ggccgtccag atggaattcc ccggtcatca gcgccagcac aagtacgcta
     9961 aacagtgccg ccagtggtgc gccacaccat gcctgcagca ccatgaagac cagcccgcta
    10021 atcgcgccaa gcaataatcc aatcaaagga aaagtaataa taccgcgaga ataatgctcg
    10081 aaatccagtc cctgggacca gcgacgcggt acaggcagac gcgtaataaa tgagagcatc
    10141 gcccaaaata atttgctcat ttgattttta ctccaatacc cgaaaccacc agccatactt
    10201 catttgccgc agcggccaac tgctgattta cccgcccggc aatatcacg
//
LOCUS       DAJVPP010000128        10075 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVPP010000128 DAJVPP010000000
VERSION     DAJVPP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.128"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1861)
                     /locus_tag="OQM34_003481"
     CDS             complement(<1..1861)
                     /locus_tag="OQM34_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCS1453778.1"
                     /translation="MNISEVDLRKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAEYHELYCAGHLIEAGVAFFQATGRRRLLE
                     VVCRLADHIDSVFGPNEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SYASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAP"
     gene            complement(1872..3272)
                     /locus_tag="OQM34_003482"
     CDS             complement(1872..3272)
                     /locus_tag="OQM34_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1453779.1"
                     /translation="MTSTPITTADIANGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGHGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            3498..4313
                     /locus_tag="OQM34_003483"
     CDS             3498..4313
                     /locus_tag="OQM34_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1453780.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFQKHTGLTPAAWKRRYSKEHINS"
     gene            complement(4345..5040)
                     /gene="araD"
                     /locus_tag="OQM34_003484"
     CDS             complement(4345..5040)
                     /gene="araD"
                     /locus_tag="OQM34_003484"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1453781.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETYEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(5034..5894)
                     /locus_tag="OQM34_003485"
     CDS             complement(5034..5894)
                     /locus_tag="OQM34_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCS1453782.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAAAQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKALHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(5887..6549)
                     /gene="ulaD"
                     /locus_tag="OQM34_003486"
     CDS             complement(5887..6549)
                     /gene="ulaD"
                     /locus_tag="OQM34_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1453783.1"
                     /translation="MSRPLLQLAPDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     SAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHTMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(6546..8042)
                     /gene="lyxK"
                     /locus_tag="OQM34_003487"
     CDS             complement(6546..8042)
                     /gene="lyxK"
                     /locus_tag="OQM34_003487"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HCS1453784.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVFTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKVG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTLAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(8046..9032)
                     /gene="yiaO"
                     /locus_tag="OQM34_003488"
     CDS             complement(8046..9032)
                     /gene="yiaO"
                     /locus_tag="OQM34_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HCS1453785.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEAITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(9045..>10075)
                     /locus_tag="OQM34_003489"
     CDS             complement(9045..>10075)
                     /locus_tag="OQM34_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1453786.1"
                     /translation="KLVGHKPGGLGYVGVLAAMIMASLSGSAVADTAAVAALLVPMMR
                     SANYPVNRAAGLIASGGIIAPIIPPSIPFIIFGVSSGLSISKLFMAGIAPGIMMGATL
                     MLTWWRQASRLNLPRQQKATMQEIWHSFVSGIWALFLPVIIIGGFRSGLFTPTEAGAV
                     AAFYALFVATVIYREMTFATLWHVLIGAAKTTSVVMFLVASAQVSAWLITIAELPMMV
                     SDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTPTVLILTPVLMPLVKEAGIDPIYFGV
                     MFIINCSIGLITPPIGNVLNVISGVAKLKFDDAVRGVFPYVLVLYSLLVVFVFIPDLI
                     ILPLKWIN"
BASE COUNT         2363 a         2732 c         2727 g         2253 t
ORIGIN      
        1 ctggcgcgct gtcgtaatgc cacagcggtt gctgctctgg attgctctgt tcataccggt
       61 aacccggtgc ctggattaag atcttatggc tgaacaatcc cttgccttca aatgtcgtaa
      121 atggcgcatc ggtgggcagc cacagattat gcagtgactc gccgttgtcg gcctgttcta
      181 ggcaatacac cagcggaccg cgctgaatcg ccacttttcc ggcgacgtga cgcaccagcg
      241 ggttaccgta aacgcggcgt accggcatcg gcaaagtcag attcagcgta tcgccctcct
      301 gccattcgcg ggtaatgtgc aaataccctt tacgaatatc ctgctcgacc tcttccccat
      361 tcaatatgat ctgcggctgt gtgcaccagt ccggcagacg taaagccagc gtatgacgta
      421 ccggctgggg cgattcaacc gcaatcgtca cctgctcctg ccacggatag ttcccgctaa
      481 cccgcaggcg cagcgtgcca ttttctaccg gcacttccat gctgtttcct gcgtatatgt
      541 tgatatacaa cgcatcttca cgcggcgtgt agagataatg accaatcgag gtcagcacgc
      601 gggcgatatt tggcggacaa caagcgcagc caaaccaacg ctggcggatc ggtttaacgt
      661 gatcgtagat atggttgaat ttcagcgatt ttggatgtac ttccagcgga ttcacataga
      721 agaaatgttt gccatccagc gccatgccgc cgagcacggt gttgtacagc gcgcgctcca
      781 tcacatcggc atattgactg tcgccttcca tttccagcat tcgccgggcg aacatcatca
      841 ggccgatgga agcataactt tcggcgtaaa ccgtgtcatt cggcagatcg taatcgctag
      901 tgaacgcttc gccgctgctt tgcgagccaa tgccgccggt aatatataac tgacgctggg
      961 ccatattgtt ccacagcctc aggcagtcct gacgcttgct gtcatcgtga cttaaacgcg
     1021 cgagatgcgc gacgccggtc atcaggtaga caaaacgtac cgcgtgaccg atggcggttt
     1081 gctgttgcgc aaggggcaaa tgtgcctggc tgtaggcttt gtctttcacc atccacgccg
     1141 ggccgtaggt gtgccagtgc gaggtctgcc cgcgcttttc atattcctgg tcgtaatagt
     1201 gcggttgcgc accacgctgt tcgacaaaat agttcgtcag cgccaggtag cgcggctctt
     1261 cggtgacttc gtagagacgc atcagcgcca gttcaatttc cggatgacca ggataaccct
     1321 gcagcttgtc ttcatttgga ccaaatacgc tgtcgatatg atcggccaga cggcaaacca
     1381 cttccagcaa gcgccgccta cccgtggcct ggaagaaggc gactccggct tcaatcagat
     1441 gaccggcgca gtaaagttca tgatactccg ccagattgct ccagcgttct tcgggtgctt
     1501 ttaccgtaaa gtaagtattg agatagccgt cttcacattg ggcggaggcg atcagttcga
     1561 ttacctcgtc ggcggttttt tccagttcgg cgtccggctt ctggcacagc gaccaggcta
     1621 ccgcttccag ccatttggcg acgtcgctgt cctgaaacac catcccgtaa aattcaccct
     1681 cctgaagtcc ggcagcaatg cgaaagtttt caatcgcatg gctgggttcc gcttctggga
     1741 tacgatcgtt caaggcatcc cactgataag gaatcaccac gtcgcggacc agttgttggt
     1801 actgaccgag gaacggatcg ctgaccgtca gtttacgcag atcgacttcc gaaatgttca
     1861 tcttgtgctc cttaaggctg tacatgacgc tgacgcaggt cggtagctat gcgtgacatc
     1921 ataggattat tgagtttgca aaagcggatc gcccctgcca gcagcaggtg gaatagcgca
     1981 ggcagtaatg tttccatcgc cgcgatcccg ccaagcgaac tggcggtctg gttttccacg
     2041 ccaggctggt aggcgacaaa aataaacagc aggctgatga tcccggcgct ggaggcccag
     2101 gccagcttga tgaaaaacag attgaaggcg aagttcatgc cggaagaacg tacgcgggtt
     2161 ttccactcgc cgtagtcatc ggcaaaggcc atcaatgaga agtgcaatgg caatgtgaag
     2221 ccaagaatca cgccattacc gaggatcacc gccagccaca gagtttgata agccgggccg
     2281 gagggcagga accacatcaa caccgccagt gcagcgagga gcaggttggt gtagtaataa
     2341 attttgacgg tatcgaaacg ccgcgttaac gggctgacaa tcaccgagcc gataatggag
     2401 gcgaaggtga ccatggtgaa gaacagcgac gtgtagcccg tactgccttg taagacgtag
     2461 gtaatgaagt acatataccc accgccgcga atgttaaaga cgttaatcag caggaaagac
     2521 atgaccagca tcagcagcag ttggtcgttg ttccgcagcc cggcaagatg ttcgcgcagg
     2581 gtaaatttcc ccattgtgga gagcggcacc cgttcacgaa cccagaagaa acagcacaga
     2641 aacatcacca cggcgatggc gcacagcacg ccgacgccca attgataacc gcgcgcggca
     2701 ttaccatgac cgaagagatc taccatccac ggtaagccta ccgaaaccag aaatcccgct
     2761 acgccacaga gaacgaatcg ccaggactgg caggagatca cttcattgtg gcgggtggtc
     2821 atggtgttga tcagcgcgca gtacggtacg ttgatggcgg tataaccgac tgaaaggagc
     2881 aggtaagtac cgaacgccca ggcgattttt actcccatgc tggcatctgg cacggtgaac
     2941 gtcaggatgc cgataatgcc gatgggaatg gcaatccata gctgccacgg acgaaagcgc
     3001 ccccaacgac tttgcgtccg gtcggcaatg acgcccatca ccgggtcgga gatggcatca
     3061 aacacgcgca gtgcaataaa cagcgtgcca accagcgccg gagttaatcc aaaaacgtcg
     3121 gtgtagaaaa aggtgagaaa attcatgatc aggcaagtta ttacggtgcc gcctgcgtca
     3181 cccaggccgt agcctatttt ttcccgtaca gataacgcat cgtcaatttt accgttagcg
     3241 atatcagcgg tagtaatcgg tgtagaggtc atagatattc ctcgtgaata gggagcaatt
     3301 tttagtttta ttgaaatgac ctgattaatt ctgccgtgaa cgggaatatc aacaaggcga
     3361 gaaaagagta aaaaaaggtg acgattccga cctgaattta aaagtgtgaa atggatcagg
     3421 tgaaattaat gtttttgatt aataatcaaa aggtaatgta tttaatcatt aatgaataag
     3481 cgggaaatta catatcaatg ctcgaattat ccatgacgtt accgattaaa gtgcaaaatg
     3541 gtggattatt tatttcccgc ggcgtaggga ggcatcctgc aaggcggctg gagtcatggg
     3601 aaattatctt tgtcgaaaaa ggtcgattaa cgattcagga agaagaacgg atatttctgg
     3661 tcgatgcggg agagagttta ttactctggc cgaatcgaca acatgttggg gtggaggatt
     3721 ttcccgccga tctcaaattt tactggctgc attttgaggt taaagcgccg gatagcgacc
     3781 cgcgctggct cactcatctt agcgtgccgc aacaaacgaa ggttgccgat ccgcaggcgc
     3841 tggtggccct gtttcgccag ttcttaagcg aacaggaaaa gcatcaacgc actccggcgc
     3901 tggagtttat tgtgctgctg attttgcagc agctcacccg tgacgcgcgt cttgatgaac
     3961 aggccgatgg cgcaggcacc acccttgcct ggaaagcgca gaaactgatc cgcacgcaat
     4021 atcatcgccc gctttctacg gcgcttctgg cccgcgagct gtactgcaat gccgattatc
     4081 ttgggcgcgt ctaccggcgg gtgtttcatc tgacgttaac agaagcaatc catcgccagc
     4141 gagtgcgcga agccgaaaag ctgttaatta gcgaagcgcg atcgctcact gaagtggctg
     4201 cgttgtgcgg gtttaatgac gttggctatt tccggcagat ttttcagaaa catactggtt
     4261 taacgccagc agcgtggaag cggcgttaca gcaaggaaca tatcaattcg tagtgccggg
     4321 gcgatgaagc ccctgcgtga gggattactg cccgtaatag gcattggccc catgcttacg
     4381 caggtagtgc ttatccagca gttcgttttg catcgcaggg agctgcggcg caagctggcg
     4441 cgagaatagg cccatatagg cgcattcttc gagtactacg gcgttatgca cggcatcggc
     4501 ggcgttttta ccccatgcga acgggccgtg agaatgcacc agcaccgccg ggatttgtgc
     4561 cggactcctg ccacgttctt cgtaggtttc aatgatcact tcgccggtct gatattcata
     4621 ttcgccgtta atttcctctg cggtcatctg tcgcgtgcag gggatggcac catagaaata
     4681 atcagcgtgc gtggtgcccc aggcggggag atccagcccg gcctgcgacc agatggtggc
     4741 gtggcgcgag tgggtatgca caatgccacc gatttcggcg tagcgacggt aaagcgccag
     4801 atgcgttggc gtgtcggacg agggcttttt gctaccttcc accaccttgc cgctggctat
     4861 ctcaaccacc accatatcgt cggcggtcat cacgtcatac tcgacgccgg aaggtttgat
     4921 taccatccat tgccgcgttt cgtctaccgc gctgacattg ccccaggtga acgtcaccag
     4981 atggtgagcg ggaagcgcca gattcgccgc cagcacgtcg gctttcagtt gctctaacat
     5041 ataaatccag cctcctgcat acgcgcttca atccaacgcc gcgcctgaat aatctccagc
     5101 accggctctt tggctttttc ggtccacatc tcaatcagga aagaacctcg atagttcagt
     5161 tcatgcagcg ctttgaagat gccaacgaaa tcgacgcagc cttcgccaaa cggcacgtcg
     5221 cggaactgtc cgggactttg ctcggtaact ggctgggtgt ctttcaggtg gatcgcggcg
     5281 atacggtcaa tgcccagttt cagttcggcg ggaacatcat tgccccaggc gctgaggttg
     5341 ccgacgtccg ggtagacggt gaaccacggc gaggcgagca tctcgtccca ctttttccat
     5401 ttactgatgg agttcataaa cgcggtgtcc ataatctcca ccgccagcat cacctgcgcg
     5461 gctgctgcct gttcaaccgt ccacgccagc ccttcagcaa aacgttgccg ggtgccttca
     5521 tcgtggtctt cgtaatagac gtcgtagccc gccagttgga tggtgcgtat gccgagatcg
     5581 cgtgccagac gaatcgcttt gctcatgatc tcccgtgctc gctggcgtac cgcgtcgtca
     5641 cggctaccaa aaggaaagcg acgatgtgcg gacaggcaca tcgacgggat cccaacaccc
     5701 gtttcgatca tcgcggcaac cagcgaagtc ctttgtgcgg tgctccagtc aagacgtgaa
     5761 agccgttcgt cggtttcatc caccgacatt tcgacaaaat caaaaccgca gctttttgcc
     5821 agcaccagcc gctccggcca ggagagatct ttcgccagcg ccttttcata aatccctaac
     5881 ggatgattac gcacgcttgc ctccccagat agcgtcgatt tgcgcatgga aatcgccagc
     5941 cacctgcgcc ggatttgctg agcctgccag tgctcgcccg gcaataaacg ctttcacgcg
     6001 aatatcttta aacagcggca ggtcagcagg ggtaatcccg ccggtaatgg aaagctcaag
     6061 gccgatatcg gaaagcgcct tcatgcgtgc cagatcggct tcgccccact gttgcccgct
     6121 ggcctgtgca tcacgaccgc gatgataaat cgcctgccgc acgccaatac gatgccagtc
     6181 gcgggcgtcg tccagcgtcc agttaccgaa caactcgatc tgaatttcac caccgcagcg
     6241 ttgcgccatc gtgtggcctt tttccaccgt cgcgagcggc gcggcgcaga tgatggtcat
     6301 ccagttagcg cctgcgccaa acgcctgttg cgcgagcgtt tcaccagcgt cggcgacctt
     6361 ccagtcggca acgatgattt tgtccgggca ttgttcgcgc aaggctttca ccgcgctaag
     6421 cccttcgttt aaacagagaa tggtgcccgc ttcgacgata tcgacgctgt cttttaacag
     6481 cgtcacgtcg cgctgcgcgg cttcaagtga tgagtggtcg ggtgccagtt gcagaagtgg
     6541 tcggctcata atgtgtgctc cttgatgcgg gcgtgaaagc cctgaagtgc ggcaatgaga
     6601 tgctgatagc gttggtattt ttgttggtaa agctgatgcg cggtcatatc cggcagcagg
     6661 gtgcgtaccg ggtgctgtaa gtcacgttga gcttcgctga aatcgcgata aactccggtg
     6721 ccgacgcggg cggcaagtgc cgcgccaaaa cagccggttt cttccacctg cggcagctcg
     6781 atacgcagac cgctgacgtc cgccagcatt tgcatccaga catcggagtg cgccgggccg
     6841 ccagtgacgc gcagggtgtg cacatcagta aaacgttcgc gcatccggtt gaggtgggtc
     6901 atatggctaa acaccacccc ttcatagatg gcctgcaaca ggtgcgcgag ggtgtgaatg
     6961 gcctgcatcc cgtagaaacc gctggtcatc tcgagtccgg cgttgctgcc gtacaggaac
     7021 ggcaggaaaa agagatcgcc accgaccttc ggcaagctgg caacggcctg attgatctca
     7081 tcaaacgaga tttctcccca ctgcgcggta aaccattcga ggttgccgga agaggtaggg
     7141 ctggcttcgt gaacgataaa ttgaccatcg ttaacgtagc gaccatagac atacggatgc
     7201 gcttcaccgt cacgtaaacc gcgggttatg ccgctggtca ccgcccaggt gcccatcact
     7261 gcgttgaggg tgaattcgtc ttcgatcccg gcgcagagtg cggtgaaaac cacatcaaac
     7321 aggccgccga ctacgggcgt acccgctttc agaccggtca gtgcggctgt ctgagcggtg
     7381 atttccccgc agatttcggc agaaccgaca acaggcggca gggcgtgatt gatttcagcg
     7441 atccccagcc agtcggtgag gcacgggtca tattccccaa gactcatgtt gtagaggttg
     7501 gactcggaaa tattgctctc ttcacagcct ttgacgccgg ttaaacacca gcgcaggtag
     7561 tcgtgcgtca tcatcacgca gccaatttgc gcgtagcgtt ctggttcgtg ctctttaagc
     7621 caacgtagca gcgacaccgg atgcccggtc cacaaggttt gtcgggtcag cgggtagagt
     7681 ttttccggga tgccatcttc ttgccagcga cgaacgattt ccatcgcccg gcgatccgag
     7741 gacaaaatgg cgttaccgag cggcttgttg tttttatcca gcaaaaacaa gccttttcct
     7801 tgtgcggaga taccgatacc gacaatttgt tccccgctaa cgccggaatg ggtaagcagt
     7861 tcgcgaatga cagccatgca gcattgccac agttctgcca tatcgcgctc tgcccaacct
     7921 ggctgcgggc ttaatgcgca cagcggcagg cgctgcacgc ctgcctcccg gccttcgcgg
     7981 tcatacagcc cggctttcag ccagctaccg ccacaatcta accccagcca gtattgcgtc
     8041 atggtttatt gcacctcatc cacggctttc agtagatcag caccctgcgg aacatctttt
     8101 acaaacatgt cgcggacctg agtacccagt gcgtcgctaa aggctttgcg atcgagatcg
     8161 gtgatggctt ccacgcccgc ttctttcatg ccgtcgatga ttttttgctg atcttcggca
     8221 accagtttgc gctggtagtt acccgcttcc tgcgcagatg aaattagcgc ttgctggaac
     8281 tccgggctta agccatcaaa cttcgctttg ttgatcacca ccagaagcgg ggaataggcg
     8341 tggtgcgtca gagaaaggta cttctgcact tcgaaaaatt ttgctgacca gacgacgttg
     8401 atcgggtgtt cctgagcgtc gatagtgcgg gtttccagcc cggtatagac ttcggcaaac
     8461 ggcatcggga tcgggttagc gccaaagact ttgaatgcgg ctatattcat cgggctattg
     8521 ttggtgcgaa ttttcagccc tttcaggtcg gcgggggttt taaccggtgc gcgcgagttg
     8581 gtgacatcgc gccagccgtt ttcccagtag gccagtactt ttagtccttt accttcaagt
     8641 gaggctttca gatcatcacc gactttgccg tcgagcgttt tatgcgcgtg agcggtatcg
     8701 cggaacagga aagggacatc aagcaagttc atcactggtg ataacccggc aaagttattc
     8761 gagccggaca tttccatatc gatggtgccg ccacgtacgc cgctgatcat cgcctgcgcg
     8821 ttaccgaggg tgctatccgg aaacaatttt aatttcagct cgcctttggt tctctcctgc
     8881 aataaatcat tgaatttttt cgccgcaata tgttgcgagt cggtttgtga ggtttcataa
     8941 ccgaaacgta aagattgcgc cgccagagaa gatgtgctga atgcagccag gccagcaatg
     9001 aataatgcgt aggttacaga gcgtaatttc ataatatttt ccttttaatt aatccatttc
     9061 aaagggagga tgatgagatc gggaataaaa acaaatacga ctaataatga atataaaacc
     9121 aggacgtaag ggaaaacgcc tctgactgca tcatcgaatt tgagttttgc cacccctgaa
     9181 ataacgttaa gtacattacc gataggcggt gtaattaagc cgattgagca gttaatgata
     9241 aacatgacac cgaaataaat cggatcgatt cctgcctctt taactaaagg cattaatacc
     9301 ggggtaagaa ttaataccgt cggcgttaaa tccatgacca tgccgacaat caggatagcc
     9361 accataatga cgataaacag cagacgcggt gaatcgacca acggttgcag taaatcagaa
     9421 accatcatcg gcagttcagc aatggtaatc agccaggcgg aaacttgtgc cgaggccacc
     9481 agaaacataa ccactgaggt ggttttcgcc gcgccaatga gtacatgcca aagtgtggcg
     9541 aaggtcattt cacggtaaat aactgtggcg acaaacagcg catagaaagc agcaacggcc
     9601 ccggcttcgg taggggtaaa cagaccggag cgaaacccgc caataatgat gactggaaga
     9661 aacagcgccc agataccgga gacaaaagag tgccagattt cctgcatcgt tgctttttgc
     9721 tggcggggta aattaaggcg actggcctgc cgccaccagg taagcatcag cgttgcgccc
     9781 atcattatgc caggggcaat gcccgccata aacagcttac tgatggataa tccgctggaa
     9841 acaccgaaga taataaacgg aatggaaggg ggaataattg gcgcgataat gccgccagag
     9901 gcaatcagcc ccgccgcccg gttgaccggg tagttggcgc tgcgcatcat cggcaccagt
     9961 agagcggcaa cggcggcggt atccgcaacg gcagaaccag aaaggctcgc cataatcata
    10021 gctgccagca cgccgacgta gcccagcccg cctggtttat gccccaccag tttca
//
LOCUS       DAJVPP010000129        10062 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVPP010000129 DAJVPP010000000
VERSION     DAJVPP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.129"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1081)
                     /gene="lysC"
                     /locus_tag="OQM34_003490"
     CDS             complement(<1..1081)
                     /gene="lysC"
                     /locus_tag="OQM34_003490"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1453787.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTS"
     gene            1606..3255
                     /gene="pgi"
                     /locus_tag="OQM34_003491"
     CDS             1606..3255
                     /gene="pgi"
                     /locus_tag="OQM34_003491"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1453788.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKIFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            3717..3959
                     /locus_tag="OQM34_003492"
     CDS             3717..3959
                     /locus_tag="OQM34_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453789.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4073..4711
                     /locus_tag="OQM34_003493"
     CDS             4073..4711
                     /locus_tag="OQM34_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1453790.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            4708..5445
                     /locus_tag="OQM34_003494"
     CDS             4708..5445
                     /locus_tag="OQM34_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1453791.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5445..7541
                     /locus_tag="OQM34_003495"
     CDS             5445..7541
                     /locus_tag="OQM34_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1453792.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7588..7866)
                     /locus_tag="OQM34_003496"
     CDS             complement(7588..7866)
                     /locus_tag="OQM34_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453793.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            7867..8010
                     /locus_tag="OQM34_003497"
     CDS             7867..8010
                     /locus_tag="OQM34_003497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453794.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8136..8546
                     /gene="psiE"
                     /locus_tag="OQM34_003498"
     CDS             8136..8546
                     /gene="psiE"
                     /locus_tag="OQM34_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS1453795.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8590..>10062)
                     /gene="xylE"
                     /locus_tag="OQM34_003499"
     CDS             complement(8590..>10062)
                     /gene="xylE"
                     /locus_tag="OQM34_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCS1453796.1"
                     /translation="NTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVAP
                     QNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGSA
                     WPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRG
                     KLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLYT
                     VPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGV
                     IVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMT
                     VDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCWV
                     LLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCM
                     GVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2520 a         2583 c         2641 g         2318 t
ORIGIN      
        1 tggaggttga accggtggta tcaagggtta atgccacgct cacttctgac gtggtgatta
       61 agtctaccga aatattatgc cgcgcgagga tgccgaaaac ttccgcgagg aagccgcgag
      121 aatgcagcat attcaggctg tgcaaagtga gcagagtctg attgcgacga agcgccagcg
      181 cgcggaacag cggcggattt tcagttttat tgcacaccag cgtaccacct gcgcgtgggt
      241 ctttgctgga gccgacaaag accgggatat cgctgcgtac tgcgggtagc aacgttgccg
      301 gatgcagtac ttttgcacca aaagttgcca tctctgccgc ttcggcaaac gcgatttcat
      361 caatgcgttt tgctgcggaa actacgcgtg gatcggtggt gtagatgccc gggacgtcgg
      421 tccagatatc aacacgagat gcgtgtaaag cctccgccag caaggctgcc gtataatcgc
      481 tgcctccacg gccaagcgtc gttgtacgac ctttattttc gctaccgata aatccctggg
      541 tgatcactaa gccttcattg aggcgtggga gcagctgcag cgcggccagt tccgccagcg
      601 cggctacatc tggctctgca cgaccaaatc ggtcgttggt acgcatcact ttacgtacat
      661 caaaccactg tgcctgaaca tcgcgttcgc gcaggatttc gacaaacagc agggtcgaca
      721 tcagctcgcc gtggctgacc agctcatctg tcagcgccgg agacgttgcc agcgccgccg
      781 cttctgccag aacagtaatg ttctccagca gacgttcaat ctcttcacgg ataacgttcg
      841 ggtaacgcag acgttccaga atggcaaact ggatgttgcg aatagcgtcg agtttttcga
      901 atcgctcgcc aggttccagc ccttccgcta aagcgaccag cagattagtg ataccagcag
      961 aagccgagag gactactaaa cgcacgttgg catcagaaag cacaatatca gcgctgcggt
     1021 tcatggcgtc aaaatcagct acgctggtac cgccaaattt ggagacaaca atttcagaca
     1081 taactacctc gtgtcagggg atccattttc agccttggca caagggaaga gcggaagacg
     1141 ggtgggcgca gagcgatact tcgctactat tttcacccag aagtgctcca ccacttgcga
     1201 aacgcccgac tgcgaacgct tctggtgaca acccagggga ttcagcccct gtagccgatg
     1261 atgaacgtgg ccagccgttc aatcacctcg gcgatgcacc ccctcaggtg ttatcacagg
     1321 actggctcct ccaacaccgt tacttgggca acgcgcctct tctggcctgc gctagcgcag
     1381 gtagtacatt tataaataaa gggtgagcgg ggcggttgtc aacgacgggg tcatgcggat
     1441 ttttcatcca ctcctggcgg tcagtagttc agctaataaa tgcttcactg cgctaagggt
     1501 ttacactcaa cattacacta acggcactaa aaccatcaca tttttctgtg actggcgcta
     1561 caatcttcca aagtcacaat tctcaaaatc agaagagtat tgctaatgaa aaacatcaat
     1621 ccaacgcaga ccgctgcctg gcaggcacta cagaaacact tcgatgaaat gaaagacgtt
     1681 acgatcgccg atctttttgc taaagacggc gatcgttttt ctaagttctc cgcaaccttc
     1741 gacgatcaga tgctggtgga ttactccaaa aaccgcatca ctgaagagac gctggcgaaa
     1801 ttacaggatc tggcgaaaga gtgcgatctg gcgggcgcga ttaagtcgat gttctctggc
     1861 gagaagatca accgcactga aaaccgcgcc gtgctgcacg tagcgctgcg taatcgtagc
     1921 aataccccga ttctggttga tggcaaagat gttatgccag aagtcaacgc ggtgctggag
     1981 aagatgaaaa tcttctcgga agcgattatc tccggtgagt ggaagggtta taccggcaaa
     2041 gcaatcactg acgtagtgaa catcgggatc ggcggttctg acctcggccc gtacatggtg
     2101 accgaagcac tgcgtccgta caaaaaccac ctgaacatgc actttgtttc taacgtcgat
     2161 gggactcaca tctcggaagt gctgaaaaaa gtaaacccgg aaaccacgct gttcctggta
     2221 gcatctaaaa ccttcaccac tcaggaaacc atgaccaacg cccatagcgc gcgtgactgg
     2281 ttcctgaaag cggcaggtga tgaaaaacac gttgcgaaac attttgcggc gctctcaacc
     2341 aatgccaaag ccgttggcga gtttggtatt gatactgcca acatgttcga gttctgggac
     2401 tgggtcggcg gccgttactc tttgtggtca gcgattggcc tgtcgattgt tctctccatc
     2461 ggctttgata acttcgttga actgctttct ggcgcacacg caatggacaa gcatttctcc
     2521 accacgcctg ccgagaaaaa cctgcctgtc ctgctggcgc tgattggcat ctggtacaac
     2581 aatttctttg gtgcggaaac tgaagcgatt ctgccgtatg accagtatat gcaccgtttc
     2641 gcggcgtact tccagcaggg caatatggag tccaacggta agtatgttga ccgtaacggt
     2701 aacgttgtgg attaccagac tggcccgatt atctggggtg aaccaggcac taacggtcag
     2761 cacgcgttct accagctgat ccaccaggga accaaaatgg taccgtgcga tttcatcgcc
     2821 ccggctatca cccataaccc gctctctgat caccaccaga aactgctgtc taacttcttc
     2881 gcccagaccg aagcgctggc gtttggtaaa tcccgcgaag tggttgagca ggaatatcgc
     2941 gatcagggta aagatccggc aacgcttgac tacgtggtgc cgttcaaagt gttcgaaggt
     3001 aaccgcccga ccaactccat cctgctgcgc gaaatcactc cgttcagcct gggtgcgttg
     3061 attgcgctgt atgagcacaa aatctttact cagggcgtga tcctgaacat cttcaccttc
     3121 gaccagtggg gcgtggagct gggtaaacaa ctggcaaacc gtattctgcc agagctgaaa
     3181 gatgataaag aaatcagcag ccacgatagc tcgaccaatg gtctgattaa ccgctataaa
     3241 gcgtggcgcg gttaatcatc atgcgtgcct gatgccgata tgtaggccgg ataaggcgtt
     3301 tacgccgcat ccggcaaccg gagcctgatg ccaatatcga cattaatgca tagtatgagg
     3361 agttaaataa ccttaaaaac aatgccgaca ataagtcggc attgttttat cagataaatc
     3421 cccttgtctg tgatttaacg gaaatcatac cgtgaggtta atcctaaaat agatttttaa
     3481 tcgttgttta tttcggagaa tacgcagatt aattactttt gtttttattt taagcttatg
     3541 atttttattg ttatttaaat ataagttgaa acttatattt gatattcatt ccaattatcc
     3601 taaaactcca tcgctaattc cccgcgccgt aattcgcatg ctttagttgt gtatactcga
     3661 tcccgcccga aatgtttttg ggtaaatctc cattcattca atgaagggaa attgttatga
     3721 aaaaagttct gtatggcatt tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg
     3781 cacctgtaca ggtgggcgaa gcggcagagt cggcagcaac gtcggtttcg gcggggagtt
     3841 cctccgcgac cagcgtcagc accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg
     3901 gtggcggtga tggttctaat accgggacca tcacaaccac gaccaccagt acccagtaat
     3961 aaagtatgta tccccaaaat aatttgagtc attgcatctg tggcttgaag tatgaaggga
     4021 tttaccataa ccacactccg gtgtggttat tctgcccctc tggagaagag tcgtgaagcg
     4081 acctgcactc attcttatct gcctgctatt acaggcctgt tcagccacga ctaaagagct
     4141 gggcaattca ctgtgggaca gtctgttcgg cacgccaggc gtacagctga cggacgatga
     4201 tattcaaaat atgccctacg ccagccagta catgcagctt aatggcgggc cgcaattgtt
     4261 tgtggtgctg gccttcgctg aagacggaca acaaaaatgg gtcactcagg atcaagccac
     4321 tcttgtcaca caacatggtc gtctggtgaa gactttgctt ggcggcgaca acctgattga
     4381 agtgaataac cttgccgccg acccgctgat taaacccgca caaattgttg atggtgcaac
     4441 atggacgcgc acgatgggct ggaccgagta ccagcaggta cgctacgcca ccgcacgctc
     4501 agtcttcaaa tgggatggca ccgataccgt caaggtcggc agcgatgaaa ccctggttcg
     4561 cgtgctggac gaagaagtct ccaccgacca ggcgcgctgg cataaccgct actggatcga
     4621 cagcgaaggg caaattcgcc agtcggaaca gtatctcggc gcggattatt tcccggtgaa
     4681 aaccactctc atcaaggcgg caaaacaatg attaaacaaa ctattgtcgc gttgcttttg
     4741 agtgtgggag cgtcatcggt cttcgcggca ggaaccgtca aggtgttcag caatggcagc
     4801 agtgaggcca aaacgctaac gggcgcagag catttaatcg atctggtagg gcaacctcga
     4861 ctggcaaaca gctggtggcc tggtgcggtg attagcgaag agctggcaac ggcggcagca
     4921 ttgcgtcagc agcaggcgtt gctgacacgg ctggcagaac agggcgcaga ttccagcacc
     4981 gacgatgccg ccgccattaa cgccttacgc cagcaaatcc aggcattgaa agtgacaggc
     5041 aggcaaaaaa tcaatcttga tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg
     5101 ttgcagggca actacacgct gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg
     5161 cttatcagcc atcctggcaa tcagccattc actccaggtc gcgacgtggc gagctatctc
     5221 tctgaccaga gcctgctcag cggtgcggat cgcagctacg cgtgggtcgt ttacccggac
     5281 ggacgcacgc aaaaagcgcc ggtggcttac tggaacaagc gtcacgtaga gccgatgccc
     5341 ggcagcatta tttatgttgg cctcgcagat tccgtctgga gtgagacgcc tgatgccctt
     5401 aacgccgaca ttcttcagac tctgacgcag cggatacccc aataatgaaa aaaagacatc
     5461 tgcttagctt actggcgctg ggcattagca cagcttgcta cggcgaaaca tatcctgcgc
     5521 ccattggtcc gtcgcagtcg gatttcggtg gcgtaggatt attacaaact cccaccgcgc
     5581 gtatggcacg ggaaggggag ttgagtctga actatcgcga taacgatcag taccgttatt
     5641 actcagcttc agtgcaactc ttcccgtggc tggaaacaac gctgcgctac accgacgtgc
     5701 gcacccggca gtacagcagc gtcgaagcgt tctctggcga tcaaacgtat aaagataaag
     5761 ccttcgatct caaactgcgt ttgtgggaag agagttactg gctgccgcaa gtggcggttg
     5821 gtgcgcggga tatcggcggt acggggctgt ttgatgcgga atatcttgtt gccagcaaag
     5881 cctgggggcc gttcgatttt acgctcggcc tgggctgggg atatctgggc accagcggta
     5941 atgtaaaaaa tccgctctgt tcagccagtg ataaatattg ctatcgcgat aacagctaca
     6001 aacaggcggg atctatcgac ggtagccaga tgttccacgg tcctgcctca ctgtttggcg
     6061 gcgtggaata ccagacgccc tggcaaccgc tgcgtctgaa actggagtat gaaggtaata
     6121 attatcagca ggattttgcc gggaagctgg agcaaaaaag taagtttaac gtcggggcga
     6181 tttatcgcgt taccgattgg gccgacgtta accttagcta tgaacgtggc aacaccttta
     6241 tgtttggcgt cacgctgcgc accaacttta acgatctgcg cccgtcctac atcgataacg
     6301 cccgcccgca atatcaaccg cagccgcagg atgccatttt gcagcattcg gtggtggcga
     6361 atcagttaac gttgctgaaa tacaacgccg gacttgccga tccgcagatc caggcgaaag
     6421 gcgatacgct gtatgttacc ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca
     6481 tccgtgctaa tcggatcgtg atgaacgatc tgccggatgg gatcaaaacg atccgcatta
     6541 cggaaaatcg ccttaacatg ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa
     6601 atcatctcgc cggagagccg ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc
     6661 cagtggttcc gaagtccacc gagcagggct ggtatatcga caaatcacgc tttgatttcc
     6721 atatcgatcc ggtgctgaac cagtcggtcg gtggcccgga aaacttttac atgtatcagc
     6781 tgggcgtgat gggaacggca gatttgtggc tgacggacca tctactgacc accggcagcc
     6841 tgtttgcaaa tcttgccaac aactacgaca agtttaacta caccaatcca ccgcaggact
     6901 cgcacttacc gcgcgttcgt acccatgtgc gcgagtatgt gcagaacgat gtctatgtga
     6961 ataacctgca agccaactac ttccagcatc tgggcaacgg cttctacggt caggtctatg
     7021 gaggttatct cgaaaccatg tttggcggtg cgggggccga agtgttgtat cgtccgctgg
     7081 acagtaactg ggcgtttggt ctggatgcca actacgttaa acagcgcgac tggcgtagtg
     7141 caaaagatat gatgaaattc accgactaca gcgtgaaaac cggacatctg accgcctact
     7201 ggacgccatc tttcgcccag gacgtgttgg tgaaagccag cgtcgggcag tatctggcag
     7261 gggataaagg cggcacgctg gagatcgcta aacgctttga cagcggcgtg gtggtgggtg
     7321 gctatgccac gatcaccaac gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag
     7381 gcgtgtatgt ctcggtaccg ttggatctct tctcgtctgg cccgacacgc agccgtgcgg
     7441 cgattggctg gacgccgctg acgcgtgacg gtggtcagca acttgggcgt aagttccagt
     7501 tgtatgacat gaccagcgac cgtagcgtaa atttccgcta agtcatggga aaggtgccag
     7561 ttttcgcact cactggcacc ctcattctta atgacctgtt gagccatcag gaacgcagtt
     7621 cacccccggt ttacgatctg gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga
     7681 tatcggttgc aggtctggtt gcctgagcga ctgggccatc attgggctgg agacattgct
     7741 ttgcggtagg tcggctttat cagcagtacc gagcggacca gcataagcag gaagaacaga
     7801 gactgataac atcaaagcag caaaataagg cttcattttt accaccttta tcaggttacg
     7861 tttcatttgt tccagaggaa cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca
     7921 cctgaatggg tgatttttga attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg
     7981 ccaggcgcgg cttatccggc ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc
     8041 aaatccaggt tgaacaaaac atacacaaaa aatatagatc tccgtcacat ttttgcgtta
     8101 tacaggaagc tcgccactgt gaaggaggta ctgctatgac gtcactctct cgtccgcgcg
     8161 tggagtttat ctccaccatt ttgcagaccg tactcaatct tggcctgctg tgcctcggcc
     8221 tgattttggt tgtcttcctc ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc
     8281 cagaacaaac cagcaaatat gagctggtag aaggactggt ggtttacttt ctctatttcg
     8341 aatttatcgc gctgattgtg aagtactttc agtccggttt tcacttcccg ctacgttact
     8401 ttgtctatat tgggatcacc gccattgtgc gcttgatcat cgtcgatcat aaatcgccac
     8461 tggatgtgtt gatctactcg gctgcgatcc tgttactggt gatcaccctg tggctgtgta
     8521 attcgaaacg gctgaagcgg gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg
     8581 acaggaagat tacagcgtag cagtttgttg tgttttcttc gtttccggtt cccagagcgc
     8641 ttccagctcc tcaagggttt tacctttggt ttccgggaca aatttccaca taaacagtgc
     8701 tgccagaacg cccatacaac cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac
     8761 cagccaggag tttttgtcca tcatcgggaa ggtccaggag acgaagtagt tcgccagcca
     8821 ctgggccgcc accgcgattg ccagcgcttt accacgaata gcattcggga agatttccga
     8881 cagcagtacc cagcataccg gaccccagga catggcaaag gcggcaacat agaacagcat
     8941 cgacagtagc gccacaatac ccgatgcctg agtgtaaaac gcggtaccga ggctaaacat
     9001 accgattgcc attccgagtg cgccgataat ttgcagtggc ttacgaccaa atttatccac
     9061 agtcataatt gccagtacgg taaaggtgag gttgataact ccgacaataa tggtctgcaa
     9121 cagcgcgata tccgtgctgg cccccagcgt tttgaacact tccggcgcgt agtacagcac
     9181 cacattgatg ccgacaaatt gctggaagat ggagagcata acgccgatta caatcacgcc
     9241 cacgccaaac atcagcagac gaccaccggt tttgcggcca tgatccaggg agtgtttaat
     9301 ttcctgtact gcctgagttg caagcgtgtt gcccataatt ttgcgcagga tactttccgt
     9361 ctgttcttgc ttgccgcgtg acatcagcca gcgaggactt tctggcacgg tatacagcag
     9421 cattaagaac agcagtgcag ggatacattc cgaggcaaac atataacgcc tgccgtcagt
     9481 attcagccag ctggcatcac cggaacgggc aataaaatag tttacgcagt aaactaaaag
     9541 ttgcccgaaa ataatcgcaa actggttaaa tgaaaccagt tttccgcgaa tatgagctgg
     9601 agccagttcc gcaatataca ttggtgaaag cattgaggct aaaccaacgc caataccgcc
     9661 aataatacga taaataacaa attccgggac ataacctgcc agataaatag gtactgtgtt
     9721 gtccgggttt atagaggtaa aaccaagttc tggccaggca gaacctacac cagaaataaa
     9781 aaacaggaca gcagaaatct taagtgaatc acgacggccg aagcggttac tgcaataacc
     9841 accgagggca ccgccgatga tgcaaccaat cagagcgctg gctacgcaaa atcctaacag
     9901 ggagttggca gcggattcac ttaagttttg tggagcaaca aagacggtat tgagtgactc
     9961 aacagtaccg gaaataacgg cggtgtcgta gccaaataat aaaccaccta atgtagcgac
    10021 taaggtaatc gaaaatatat aactggaatt atactgggta tt
//
LOCUS       DAJVPP010000130         9885 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVPP010000130 DAJVPP010000000
VERSION     DAJVPP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.130"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..715
                     /locus_tag="OQM34_003500"
                     /pseudo
     CDS             1..715
                     /locus_tag="OQM34_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            728..1641
                     /locus_tag="OQM34_003501"
                     /pseudo
     CDS             728..1641
                     /locus_tag="OQM34_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2320..2538
                     /locus_tag="OQM34_003502"
     CDS             2320..2538
                     /locus_tag="OQM34_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCS1453797.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2756..2998
                     /locus_tag="OQM34_003503"
     CDS             2756..2998
                     /locus_tag="OQM34_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453798.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="OQM34_003504"
     CDS             complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="OQM34_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1453799.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="OQM34_003505"
     CDS             complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="OQM34_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1453800.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="OQM34_003506"
     CDS             complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="OQM34_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1453801.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="OQM34_003507"
     CDS             complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="OQM34_003507"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8264..8266),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1453802.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9045..9878
                     /gene="fdhD"
                     /locus_tag="OQM34_003508"
     CDS             9045..9878
                     /gene="fdhD"
                     /locus_tag="OQM34_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1453803.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2476 a         2389 c         2537 g         2483 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
      541 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
      601 tccgtcgatc accaataaat actcagcaaa agatgtaggt ccagatatac ttgcattgcc
      661 cgtcaacgta tatcatgcgg attttaaaat taaaatcaat aatgcagtgg agaaggcttc
      721 atcaattgtg ttatgtgctg tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg
      781 gataatagga aatgtgccaa aaaataataa accaaaaaca attatttttg atttgcgttt
      841 tcttagtgca attaagcaga aagttttttg ggcagtgaaa aaccgttgtt tgtaaagcat
      901 accgcaatga tgagggaggc atgtaaggaa ttaccgcagt cagtggaata tgttccttta
      961 gcctgtgaag cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcgcccatc
     1021 atggcgacag gtgtgggctt ggcattaatg tatgcaacat catcaggacc gtattatgcg
     1081 caaagtcttt ctggttcgcc ggatatcgag atgataaaaa atcgcatggc tgaattgttt
     1141 aaccagtcgg atatttctgt gcgcaacaaa tatagcccgg cttttgataa atggaatgaa
     1201 ctggtagata aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt
     1261 ttagcgacgg cgataaatga aactcttgag ggggatacga atgttgccgt tgtgatgggg
     1321 tgtaaatctg cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca
     1381 ctccttgaaa aacgcctcgc cgatagtgga gaaatagaaa atctttttga tcaacaggga
     1441 tattttagtt gcgattcatt aacacccgaa gaattaatga tgttaaaaga cctttttgat
     1501 attcgagttc ttcatgtctc taacaaattc aatgttggat tgcaaggtaa tatcaacact
     1561 gatgttttac aagattcgtt ttttaaaaat gtcgatttca ttcatgaata taactcgtat
     1621 accgtaggga tgggagtctg acgagtaaaa ctcattagga ttatcagaat tcggacaatg
     1681 cattgcaaaa atgacataca aagagagatg tgtgcaggta cttagccgcg aggggcatgg
     1741 cgaagccata cagaaagtac cagtattcag aactccagtg aaagcggcat agtggccgcc
     1801 tgctcaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct atgatgcgat
     1861 taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga tgccgggggt
     1921 gttccggaag gaattccttg tttcccgaac tgttaccaac aaaattataa cgatgagcaa
     1981 gtaaagaccg gttggtggca gtactgaccg gtctgtgatg aaagattaat ttcaccttaa
     2041 atcctggtgc tgttaggatg ctgacgtttt tcggctacac cattcagatt atgcaaataa
     2101 atatccctga atataatata ttcatagcac ccataactca tcctgggcga taactaaacc
     2161 atttttgagt aataaatgaa acaactgcaa cgctcttatt atttctggaa aacgcctcgc
     2221 aaaaacaaaa tgacatgcaa aaatccgcat ttttttaaat gcttttccgg cgtaattttc
     2281 gcacatacat taatgagtaa tatatgtaag gtggtggtaa tggcgatgaa tacggttttt
     2341 cttcatttat cagaagaggc aattaaacgg ctgaacaagc ttcggggctg gcgtaaggtt
     2401 tcacgatctg caattttacg cgaagcggtt gagcaatatc tggagcggca gcaatttccg
     2461 gtgcgtaaag caaagggagg caggcaaaag ggggaggtag ttggtgttga tgatcagtgt
     2521 aaggagcata aggaatgaaa aaaatttttc attcttgaat ataaaaaaca gatgccctta
     2581 ttctggtatt aatacaaggc tgttttactt gaacttataa taactgcaac tgttacatcg
     2641 tatctggaaa acgcctcgca aaaataaaaa atgatgcgta aatgagcttt ctttaaagat
     2701 tcggcaaggg tacttttctc tccatagatt caaaaatgat attggcgaga tatttatgaa
     2761 ctctctggca ggtatagata tgggcagaat tttactcgat ttatcgaatg aggtgattaa
     2821 gcaacttgat gatctcgagg tgcagcgtaa tcttcctcgt gcggacctat taagggaagc
     2881 ggtagatcaa tacctgataa atcaatcgca aacagcaaga accagtgttc ctggcatctg
     2941 gcaagggtgt gaggaagatg gtgtcgaata tcagcgtaag ctgcgcgagg aatggtaatg
     3001 ggaaaaaatg atgtcaatca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
     3061 gtgaacgcct tatccggcct acaaagtcat gcaaattcaa tatattgaag tttattggta
     3121 ggcctgataa gcgcagcgca tcaggcaatc atacgcttgt catcagtgac atacccataa
     3181 ccttatcgat gtgttggagg gaagaacgag gcaaacagac aattgcggca tgatcttgaa
     3241 tgggtaacgc ggaatacgag agatggtgtc gtcacaccct atgagctgta atccgggcga
     3301 cctgcgccac ccggaaaacg tcagagatta ctctccttca cccggaaaca ggaacgggtt
     3361 aatggaacta cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag
     3421 gtcatctgcc acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt
     3481 gccgcagtca tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta
     3541 atgcagtttg ccgctctgct cgcagttgct gcattttacg cgcactacgt gccattcggt
     3601 ttcacacagg ttgcagtgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct
     3661 ggacaccggc atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg
     3721 ggctttgccg gggatcagat tggccatctg cgcccagtag agcgacagcg cagcccagat
     3781 aaacggtgct ttatcgctgc tgacggacga gaaatcagag gcaaacagcg cgctggccat
     3841 atcttccagc tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc
     3901 gctcatttca ggtttcagct cagcaattag cgccatcagc agcttttgcc agtgcttatc
     3961 acgcggcaga acgtgaatat ccagcggggg cttgccctgt gcgctggctt ctttaatgcg
     4021 cgcggtcaga tccatctcca gcggatggtc gtacagcacc actttctggg cgtgggcgat
     4081 aagcgcagca aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg
     4141 ctcggcgcgg cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc
     4201 cgccgtacgt ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca
     4261 gctttctttt ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc
     4321 caggcgctgg taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg
     4381 gcgtagatat gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt
     4441 aacgcgaatc ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc
     4501 acgccgctca ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga
     4561 ccgaagttat aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc
     4621 ttcgcccaaa agatatcatc ccgattgatt aggttttgat gccagtaacg gaaaaacatg
     4681 atgatgaacg aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc
     4741 ggtgtgccca tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc
     4801 accgccgcca ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg
     4861 tagcgcacga tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat
     4921 tattctcttc ctcatccgca cggttcggac cgacacccac gtagtggaag atactggccg
     4981 caaaggtagc cgcaaagcca acagccgcga gcggtttcca gatgcctttc cagaatttca
     5041 cggtttcgct gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat
     5101 ggtgcagcac gtacatgacg tgtgtaccac cgacgcctgc cggatcgtac agacccgcat
     5161 tgtcgtaacc gcgggttttc agctcagcaa cgcgctcgct cgccagcgtt ttcatcgact
     5221 ctttcgtgcc gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc
     5281 caaccaccac gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga
     5341 ggcgcggaat gtcgaacgga cagcccgcaa tgcaataacc gcagccaatg cactgctcgg
     5401 actggaagtc gacgataccg ttggcatact gaataattgc accttccgcc gggcacgctt
     5461 tcaggcagcc tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt
     5521 tgtcgttctg ctccacttcc gagaagcgca ttaccgtcca cgatttggcg cttaaatcat
     5581 tggggttgtc gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg
     5641 aacacgccac ctgacaggct ttacagccga tacaggtggt aacgtcgatg agtttcgcca
     5701 cttcttcctg gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac
     5761 gaatgatatc ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca
     5821 caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc accaacgaat ggcgttaaag
     5881 tattggcaat aaagcctttt ttagctacgc cttcatagcc ccagtgaatc ggaataccga
     5941 tggtatcgat atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt
     6001 tggctttgat atagccacgg ttggaggaga ctttcacggt atcgccctgg gcaatgccaa
     6061 gtttattcgc cagcgactcc ccgatttcca caaactgctc tggctgcaaa atcgcgttca
     6121 acagcgcgtg tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat
     6181 acgggaactt atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagctg
     6241 ggttcgagat aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt
     6301 agtgttccgg gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt
     6361 cctgctgcat gataaacggc ccgacgccgc tacccggagg cgctgcgctg tagtccggaa
     6421 tatcccagcc ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg
     6481 ggttaccctg cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg
     6541 cccatgccca gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca
     6601 tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca gcagccacag gaggttgtac
     6661 cgtcatcgcg caattgggcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac
     6721 cggttgccgg atcggtaata tcggccagcg ccttaccgtt gctctccatc gctacttctt
     6781 ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tgatccgggt
     6841 tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct
     6901 caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc
     6961 gacctgagtt aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc agacggaaca
     7021 cttcggtctg gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt
     7081 tagaggtttc agtgttcagt gggtcgatgg tgacgaggaa cttcagtttc gacagacaac
     7141 cgatcacttt gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc
     7201 cattgacctt gccctctttc atcatctcga agtattgcag gacgtcgtag cctttatccc
     7261 acttcggcaa ccagtcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg
     7321 ccttcatcat agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca
     7381 gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg
     7441 gcagcgtcat gtaacctggc agactctgcg acagtagccc caggtccgtc agcccctgaa
     7501 tattggagtg accgcgcagg gcgttaacgc cgccgccggc catccccata ttgccgagca
     7561 gcagctggat catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc
     7621 cgagggcata caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc
     7681 agactttcag gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt
     7741 agcgggaaac gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat
     7801 cgcgtttggc gaagccgttt tcgtccagtt cataagtcca ggaggattta tcgtacttgc
     7861 gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga
     7921 tcaggctggc gttggtatag gcttcggtgt attcgtggtt gaatttttca ttgttcagca
     7981 ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt
     8041 agtcagctac cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccgt
     8101 tgtgaatttt ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac
     8161 cgcccatcac cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac
     8221 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt
     8281 tgtcgaccgc gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt
     8341 tactcgacgc ggaagcacac agcatcccgg tggagagcca gcggttaaca gtcacgcctt
     8401 cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat
     8461 caaacgcctc ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg
     8521 gaaacttcag acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa
     8581 gtgcaccgcg gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg
     8641 cgttttttgc tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg
     8701 tgcaggtatt acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg
     8761 ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga
     8821 tcttaaagaa ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg
     8881 tcccatttac attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag
     8941 gggccgcaaa ggtccagaat tggctcaatt tccctccatc taggaagggt ttgtaacaga
     9001 ataccataat gttggtgtgt atgttcttat ctggttaaga gaaagtgaaa aaaacacagc
     9061 gaaaagaaat cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc
     9121 gtgatgattt acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg
     9181 tctacaacgg catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg
     9241 cgctcggttt ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg
     9301 atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga gctttccagc cgccgcttta
     9361 tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg
     9421 tggagcaact taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg
     9481 atctcaacaa actggatgat gcattacgtc atctcaacga ttttcagcca gtggggcgac
     9541 tgactggttg tactcacgcc gctgcctgga tgttgccatc tggcgaactg gtcggcgggc
     9601 atgaagacgt gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag
     9661 gggaaagctg gcagcaaggt gcggtactgg tttccagtcg tgccagttat gaaatggtgc
     9721 aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacgcttg
     9781 ctgtagaagt ggctgagcgc tgtaatctga cactggtagg tttttgtaaa ccgggtcggg
     9841 caacggttta tacccatccg cagcgtttga gcaattaatt aaaat
//
LOCUS       DAJVPP010000131         9695 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVPP010000131 DAJVPP010000000
VERSION     DAJVPP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.131"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            39..170
                     /locus_tag="OQM34_003509"
     CDS             39..170
                     /locus_tag="OQM34_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCS1453804.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            430..1425
                     /gene="pgl"
                     /locus_tag="OQM34_003510"
     CDS             430..1425
                     /gene="pgl"
                     /locus_tag="OQM34_003510"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1453805.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1466..2419)
                     /locus_tag="OQM34_003511"
                     /pseudo
     CDS             complement(1466..2419)
                     /locus_tag="OQM34_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2603..3655
                     /locus_tag="OQM34_003512"
     CDS             2603..3655
                     /locus_tag="OQM34_003512"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCS1453806.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLFGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3731..5164
                     /locus_tag="OQM34_003513"
     CDS             3731..5164
                     /locus_tag="OQM34_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCS1453807.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5347..7527
                     /locus_tag="OQM34_003514"
     CDS             5347..7527
                     /locus_tag="OQM34_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1453808.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGLQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7668..8951)
                     /locus_tag="OQM34_003515"
     CDS             complement(7668..8951)
                     /locus_tag="OQM34_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1453809.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9086..>9695)
                     /locus_tag="OQM34_003516"
     CDS             complement(9086..>9695)
                     /locus_tag="OQM34_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1453810.1"
                     /translation="IAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCW
                     LRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMK
                     ETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLS
                     ARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2364 a         2267 c         2470 g         2594 t
ORIGIN      
        1 acttttgacg cagtgtcttg ttaacagggg gattgaccat gcttattggc tatgtacgcg
       61 tatcaacaaa tgaccagaat accgatctac aacgtaatgc gtttatttgt gccggaagtg
      121 agttgattgt tgcgcgaatg ggaagttggc cagaagttgt tatggcatag tgtttacggt
      181 cgttgataca ggtaaacaaa agcggggaga gtcatactcc ctgaatagtc gcgaaaatat
      241 ctgtggcatt gtcaaattcg tgtcggtgac taatatttta gtaatatgcc attcgaaatg
      301 gagtgctaac ttattgattt aattgatgtg cgatattgta ttttatcgtg ttttgttgtc
      361 tgttttttat ccagtaagtg ttgttatatg tatgaaagat aagggattta tcatttttga
      421 ggtgtgttca tgaagcaaac agtttatatc gccagccctg agagccagca aattcacgtc
      481 tggaacctga atcatgaagg cgcactgacg ctgacacagg ttgtcgatgt gccggggcag
      541 gtgcagccga tggtggtcag cccggacaaa cgttatctct atgttggtgt tcgccctgag
      601 tttcgcgtcc tggcgtatcg tattgccccg gacgatggcg cactgacctt tgccgcagag
      661 tctgtgctgc cgggtagtcc gacgcatatt tccaccgatc accaggggca gtttgtcttt
      721 gtaggttctt acaatgcggg taacgtgagc gtaacgcgtc tggaagatgg cctgccagtg
      781 ggcgtcgtcg atgtagtcga ggggctggac ggttgccatt ccgccaatat ctcaccggac
      841 aaccgtacgc tgtgggttcc ggcattaaag caggatcgca tttgcctgtt tacggtcagc
      901 gatgatggtc atctggtggc gcaggaccct gcggaagtga ctaccgttga aggggccggc
      961 ccgcgtcata tggtattcca tccaaacgaa caatatgcgt attgcgtcaa tgagttaaac
     1021 agctcagtgg atgtctggga actgaaagat ccgcacggca atatcgaatg tgtccagacg
     1081 ctggatatga tgccggaaaa cttctccgac acccgttggg cggctgatat tcatatcacc
     1141 ccggatggtc gccatttata cgcctgcgac cgtaccgcca gtctgattac cgttttcagc
     1201 gtttcggaag atggcagcgt gttgagtaaa gaaggcttcc agccaacgga aacccagccg
     1261 cgcggcttca atgttgatta cagcggcaag tatctgattg ccgctgggca aaaatctcac
     1321 cacatctcgg tatacgaaat tgttggcgag caggggctac tgcatgaaaa aggccgctat
     1381 gcggtcgggc agggaccaat gtgggtggtg gttaacgcac actaaccgct gatttaaccg
     1441 gcgcagtctc tcctgcgccg gtgtattaac ttatctcctg taacgcatgt ctctggcgtt
     1501 cgacgatatt ggtccacaaa ttgtctttat cgtcagtcca caaattaatc agcaaggaaa
     1561 aaaagcgttc agctgccgga gaaagcacgg catctttgcg gcgaataatc cccaatgtcc
     1621 gacgtataac gggttccacc agtgggatac caataatcga agaatagggc gcatggggca
     1681 ttgccagacc aggaagcgcc gaaatcccca gtcctgcctc cactagtcct aatgacgtcg
     1741 aaagatgacg cacttcgtaa aaccagtcca gcttccaggg cttgtcggct agctgttgtt
     1801 ctatcaacaa tcggttgccg ctggaggaac gtacgccaat cattttgtaa cccactaact
     1861 cctgccattc tacaagctac tttttggcca atggatggtc acgccgacag gccagcacga
     1921 atggctcgtt gaccagtggg gtaaaatcaa tggatgaatt tgtgacgttg ttcatgttta
     1981 tgccaaagtc tgattcgtta cacagcaccg attccatgca attattcgtg ccttgttcca
     2041 gaatccgcac tttaatattg ggatacagct cattaaattt accgattgcc agcggtaaaa
     2101 aataaaatac tgcggtcgga atacacgcta atgtcaccat accacgatga taagcattca
     2161 tatcacgaat attaaaaagc gtttcatcga atttctttat taattcccgc gcctccggga
     2221 gcaacctttt tcctgctttt gttaacgtaa ccttgcgagt tgtacgctca aatagttgaa
     2281 tatgtaaatc ttcctccatc ttttttattc ttcgcgttaa tgcaggttgc gtaatattga
     2341 gtaatttagc ggcattgtta aatgaactgg actctgccag tatgacaaat gccttcatac
     2401 ttgataattc atgcttcatt attactccgg aaaatggaag cgacgatttt ggggggctgg
     2461 tcgttaaaaa ttttaactgc atttagccaa cttaaattaa tgaaaaaatg ttattaatcg
     2521 ttgagctaaa gtcattagag atgctttgtc cttaatgtaa ccatatcgca ataagttatg
     2581 tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc gaggtggaac
     2641 ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa gatcaaacgc agcgcgataa
     2701 aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta ttggcggcgg
     2761 taatccactg accagtaaag tcgccattat tagccgttcc agcgatccgc gtgctgatgt
     2821 cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca cgcctaactg
     2881 cggcaatatg ctgtttggcg taggggcatt cgccattgaa aatggtttga ttgcagcgac
     2941 ttcgccagtt acccgtgtac gtatccgcaa cgtcaatacg ggtacgttca tcgaagctga
     3001 tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg acggcgtacc
     3061 gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gctggaacga aaaccggaaa
     3121 agttttcccg actgataatc agattgatta ttttgacgat gtcccggtga cctgtatcga
     3181 tatggcgatg ccagtcgtca ttattccggc tgaatatctg ggaaaaacag gttatgaatt
     3241 accggcggaa ctggatgccg acaaagcatt attagcccgc attgaatcta tccggctaca
     3301 agcgggtaaa gcaatgggct taggtgatgt cagtaatatg gttatcccta aacctgtgct
     3361 tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc cgcattcttg
     3421 ccatcgcgcg ctggcgataa ccggtgctat tgctatttcc agtagttgtg cattggaagg
     3481 caccgtcacc cgacaaatcg tcccttctgt aggatatggc aatatcaata ttgaacaccc
     3541 cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga cgttacgcgc
     3601 atctgttatt cggacaacca gaaaaatatt ttccggtgaa gtttatcttc cctgaaaaaa
     3661 ttcgttgtca ggataaggac aatcaataaa ggacttctgt atgagtcata cagaaagaac
     3721 aggattttaa atgaataaga aatcgttatg gaagctaatt ctgatattag cgatcccatg
     3781 tattattggt tttatgccag ctccggcagg attaagcgaa ctggcgtggg tgctttttgg
     3841 tatttacctg gcggccattg tgggtctggt tatcaagcct ttcccggaac ctgtcgtact
     3901 gttaattgcc gttgctgcct caatggtggt ggtcggtaac ttatccgacg gtgcgtttaa
     3961 gaccaccgcc gtattaagcg gttactcttc aggtactacc tggctggtgt tctcggcgtt
     4021 taccttaagc gccgcatttg tgaccaccgg tttaggtaaa cttattgcct atctgctgat
     4081 tggtaaaatc ggtaacacca tgctgggtct gggttacgtt acggtattcc tcgatctggt
     4141 actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcattgtgt taccgatcat
     4201 caacagcgtg gcggtggctt tggggtccga accggaaaaa agtccgcgtc gtgtcggaca
     4261 ttacctgatg atgtccattt acatggtcac caaaaccacc agctatatgt tctttaccgc
     4321 aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc tgcaaattag
     4381 ctggggtgga tgggcgctgg ccgccggatt gccgggcatc attatgctgc tggtcacccc
     4441 gctggtaatt tacaccatgt atccaccaga aattaaaaag gtggataaca aaaccatcgc
     4501 taaagcgggc cttgccgaac tgggaccgat gaaaatccgc gaaaaaatgc tgctcggtgt
     4561 ctttgtgctg gcgctactgg gctggatttt cagtaagtct ctgggggttg atgaatccac
     4621 tgtggcaatc gttgttatgg cgaccatgct gctgctgggt atcgttacct gggaagacgt
     4681 ggttaaaaat aaaggcggct ggaatacctt aatctggtac ggcggtatta tcggcttaag
     4741 ctccttatta tcgaaagtta aatttttcga atggttagct gaagtcttta aaaataacct
     4801 ggcatttgat ggtcacggta acgttgcttt cttcgttatt attttcctca gcatcatcgt
     4861 gcgttatttc ttcgcttccg gtagtgccta tatcgttgct atgttacctg tatttgccat
     4921 gctggcgaac gtctccggcg caccgttaat gttaaccgcg ctggcactgt tgttctccaa
     4981 ctcctatggc ggcatggtta ctcactatgg cggcgcggca ggtccggtca tctttggcgt
     5041 gggttacaac gatattaaat cctggtggtt ggtcggtgcg gtactgacga tattaacctt
     5101 cttggtgcat atcaccctcg gcgtgtggtg gtggaatatg ctgatcggct ggaacatgct
     5161 gtaaatatac tcgtcatact tcaagttgca tgtgctgcgt ctgcgttcgc tcaccccagt
     5221 cacttactta tgtaagctcc tggggattca ctcacttgtc gccttcctgc aactcgaatt
     5281 atttagagta tatccattta ttatctttct gcgcacttca cggtgcgcag atatctggag
     5341 catttgatga tcaagttatc tgaaaaaggc gtgtttctcg ccagtaataa cgaaataatt
     5401 gccgaagaac atttcaccgg cgaaattaaa aaagaagaag caaaaaaagg cactattgcc
     5461 tggtctattc tctcttctca taatacgtcc ggaaatatgg ataaacttaa aattaagttt
     5521 gattcattag cctctcacga tattaccttt gttggtattg tacagaccgc taaagcgtcc
     5581 ggaatggaac gtttcccgct gccgtatgtg ctgaccaact gccataactc actctgcgcc
     5641 gtcggcggca ccattaacgg tgatgaccat gtttttggtt tatcggcagc tcagcgttat
     5701 ggcggtattt ttgtgcctcc gcatattgcg gtcatccatc aatatatgcg tgagatgatg
     5761 gctggcgggg gcaaaatgat cctcgggtca gacagccaca cccgttacgg tgcattaggg
     5821 acaatggcag tcggtgaggg tggcggtgag ttggtaaaac agctgcttaa tgacacctgg
     5881 gatatcgact atccgggcgt ggttgcggtg catctgaccg gaaaacctgc gccgtatgtg
     5941 gggctacagg atgtggcgct ggctatcatt ggcgcggtgt tcaaaaacgg ttacgtcaaa
     6001 aacaaagtca tggagttcgt tggaccgggc gttagcgcgc tctctaccga tttccgtaac
     6061 agcgttgacg tgatgaccac ggaaaccacc tgtttaagct ccgtctggca aactgatgaa
     6121 gaagtgcata actggctggc gctgcacggt cgcggccagg attactgcca gcttaaccct
     6181 caaccgatgg cgtactacga tggctgcatc agcgttgatt taagcgccat caaaccaatg
     6241 attgcgctgc cgttccaccc gagcaacgtg tatgaaatcg acacactgaa ccagaacctg
     6301 accgacattc tgcgtgagat tgaaattgag tccgaacgcg tggcgcacgg taaagccaaa
     6361 ctctcgctgc tggataaagt ggaaaatggt cgcctgaaag tgcagcaggg gattatcgcg
     6421 ggctgttctg gcggtaacta cgaaaacgtc atcgcggcgg cgaatgcact gcgcggtcaa
     6481 tcctgtggca atgacacctt ctcgctggcg gtttacccgt catcacagcc ggtgtttatg
     6541 gatctcgcca aaaaaggtgt ggtagcagat ttgattggcg caggcgcaat catcagaacc
     6601 gcattctgtg gcccatgctt tggcgcgggc gatacgccaa tcaacaacgg tttgagtatt
     6661 cgccacacca cgcgtaactt cccgaaccgc gaaggctcta agccagccaa tgggcagatg
     6721 tcagcggtgg cgttgatgga cgctcgttct atcgctgcga ctgcggcaaa cggtggctat
     6781 ttaacctctg ccagcgaact tgattgctgg gacaacgtgc cggagtacgc cttcgatgta
     6841 acgccgtata aaaaccgtgt ttatcagggc tttgtgaaag gggcaactca gcaaccgctg
     6901 atttacggac cgaacattaa agactggccg gaattgggtg cgctgactga caatatcgtc
     6961 ctgaaagtgt gctcgaagat cctcgacgaa gtcaccacca ccgacgaact gattccttcc
     7021 ggtgaaacct cttcttatcg ttcaaatccg attggtctgg cggggaatgt cagcgagctg
     7081 acagaggtgt ttgcgcgcat taagcagatt gctggtcagg agcatattga tccgctgcaa
     7141 actgaaattg gcagcatggt ctatgcggtg aaaccaggcg atggttctgc gcgtgaacag
     7201 gcggcgagct gccagcgtgt gattggcggt ctggcgaata ttgccgagga gtacgcgact
     7261 aaacgctacc gttctaacgt catcaactgg gggatgttac cgctgcagat ggcggaagtg
     7321 ccaacctttg aagtggggga ttacatttac atccctggca ttaaagcggc gctggataat
     7381 ccgggtacga cgtttaaagg ttatgtgatc catgaagatg cgccggtaac ggaaattacg
     7441 ctttacatgg aaagcctgac ggctgaagag cgcgagatta tcaaggcggg tagtttgatt
     7501 aacttcaata aaaaccgtca gatgtaaaaa gcgccatgtg aatgtaggtc gcattcggca
     7561 cttattgtcg gatgcgatgc tggcgcatct tatccgacct acaaatcgca tcgaaccgta
     7621 ggccggataa ggcgtttacg ccgcatccgg caaatagtta atagctctta cttcttcgcc
     7681 tctgcaacca ctttgctgcc cacgccgcgg ttattgtatt cccacatgcg gttgtagtta
     7741 gtgtcattca gattgcgctg tatttcgtcg ttatcatcta cgctgccggt attacccgca
     7801 aacggacgat tagagatcac cgcatcggcc cacggtttag ccgtgttaaa accttcgttg
     7861 atggcgctat cacggatcac cacctgaccg ttggtattag catcaacatc cagcgagcgg
     7921 cccagttgcg ccacgccatc accggaagca ttgaaacggc tgtttacggc gaggaaaccg
     7981 taatagatgt tagacagcgt agccggtgca aacacatatg cttcttgctg ggtacgtgag
     8041 ttcaccacgc ggaattcggt gttatcgaac accactgcgc cgcgaccaga aacgatatcc
     8101 acatcccctt caatatagct gttggtcacc agcgtacgcg gctgacggtt ggtttccaga
     8161 cggttctgca caccgctgtt ggtgacaaag aaggtgttct gacgaccgag aatgttaacg
     8221 ttgttaatct gtacttggtc accgtcagta cgcagtgcta ccgccggatg gttacctgca
     8281 tctacgctat cgcccagcgt gttttcgatg gtcagatttt gcagttgcag tccattgttt
     8341 tgtgaccaga agaccgcaga acacagaaca ccgatactgt cgctgcgttt gctctggcag
     8401 ctatcgtaca tataccacgc tggtttacct ggcatatatt tgccgcgcgg gttgacgtcg
     8461 tgacgccagt cggcagggct catgccccca tcaagggaaa gcccaatctt cacatcaatc
     8521 ggtttttcac ctgtaccgta cagagtgatt ccacctggag cagcagggac atacaccgtt
     8581 ccctgatact caccaggcat cacggcaata tactggcgct tgttggtgcg cttgataatt
     8641 gccgcatcta ccgccgcctg aatcgtggta tgcgttacac cttgagtacc cgccgggccg
     8701 acaacaaagt caggttgcgc aggcagggtt atcggggacg gattccacgc tgcagcacct
     8761 ggtgtcaggg atgcaaaata gtgttgcgca tcgaaattca gcgcttcttt tgccgacaga
     8821 atcgggcgag aagaggtacc aggcgcagtt tgatcagaag gacgttgatc gggtggtgtt
     8881 gagctacagg cggtcagcgt cacgccaaaa gccaatgcca gcgccagacg ggaaactgaa
     8941 aatgtgttca caggttgctc cgggctatga aatagaaaaa tgaatccgtt gaagcctgct
     9001 tttttatact aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag
     9061 gtcattatca gtcaaaataa aatcattatt tgatttcaat tttgtcccac tccctgcctc
     9121 tgtcatcacg atactgtgat gccatggtgt ccgacttgtg cccgagaaga tgttgagcaa
     9181 acttatcgct tatctgcttc tcatagagtc ttgcagacaa actgcgcaac tcgtgaaagg
     9241 taggtggatc cccttcgaag gaaagacctg atgcttttcg tgcgcgcata aaataccttg
     9301 atactgtgcc ggatgaaagc ggttcacgac gagtagatgc aattatggtt tctccgccaa
     9361 gaatctcttt gcatttatca agtgtttcct tcattgatat cccgagagca tcaacatgca
     9421 atgttgtagg gatggcaatt tttacgcctg ttttgctttg ctcgacataa agatatccat
     9481 ctacgatatc agaccacttc atttcgcata aatcaccaac tcgttgcccg gtaacaacag
     9541 ccagttccat tgcaagtctg agccaacatg gtgatgattc tgctgcttga taaattttca
     9601 ggtattcgtc agccgtaagt cttgatctcc ttacctctga ttttgctgcg cgagtggcag
     9661 cgaccgggtt tgttgttata tggccttcag ctatt
//
LOCUS       DAJVPP010000132         9370 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVPP010000132 DAJVPP010000000
VERSION     DAJVPP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.132"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OQM34_003517"
                     /pseudo
     CDS             complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OQM34_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            764..916
                     /locus_tag="OQM34_003518"
                     /pseudo
     CDS             764..916
                     /locus_tag="OQM34_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2297..3244
                     /gene="icsP"
                     /locus_tag="OQM34_003519"
     CDS             2297..3244
                     /gene="icsP"
                     /locus_tag="OQM34_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCS1453811.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3806..4178)
                     /locus_tag="OQM34_003520"
                     /pseudo
     CDS             complement(3806..4178)
                     /locus_tag="OQM34_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4422..4541
                     /locus_tag="OQM34_003521"
                     /pseudo
     CDS             4422..4541
                     /locus_tag="OQM34_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4791..5657
                     /gene="ospB"
                     /locus_tag="OQM34_003522"
     CDS             4791..5657
                     /gene="ospB"
                     /locus_tag="OQM34_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCS1453812.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5986..6726
                     /gene="phoN2"
                     /locus_tag="OQM34_003523"
     CDS             5986..6726
                     /gene="phoN2"
                     /locus_tag="OQM34_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCS1453813.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7079..8533)
                     /gene="ospC4"
                     /locus_tag="OQM34_003524"
                     /pseudo
     CDS             complement(7079..8533)
                     /gene="ospC4"
                     /locus_tag="OQM34_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
     gene            complement(8900..9370)
                     /locus_tag="OQM34_003525"
                     /pseudo
     CDS             complement(8900..9370)
                     /locus_tag="OQM34_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2900 a         1742 c         1785 g         2943 t
ORIGIN      
        1 acaattaata tacattaagt aacaggcatt cgagccttga tttgagaaag cctgtcattc
       61 atttgtttta tgtagctatc tatttcctct gatgcataac taaaaagttc tcccatttca
      121 cgagcaataa taatctcaga tttaatagac tttaatctct ggcgactcat ctcttaacac
      181 ttgcataatc tctcttttta tatcatatag ttctataaac cgatttgccc gtagttctcc
      241 ttggggagaa tcatttctga atacaacaat tatatacaaa aaatcactat tttctttaga
      301 gaagatctta caactaatct ctgggagctc acataatgca tgtaagtcag gatggtacaa
      361 actatccaca gaaaattgcc ccataggacg ttttccagat ataactgtca accagcttcc
      421 ttccggtgta ctggcttcgg ctctggaaat aggctgaaaa tcttccggac atggagaata
      481 cattgtcttc atataattaa gtcgtctgga tttctcatta ataaactcct ctatttttaa
      541 ttgtgccata cttctggtta taccagagat atacttcttg tcatatatgt ttggtgattc
      601 attaaagtaa ccatgttcaa ctctaggata aatatttctc actatatatt cagtatttgc
      661 agtatttatt ggaaatacgt tcttaatatt cttgatggga ctaatcattt taatctctat
      721 actcaaatat aaacattacc atgaacaata taattgttcc taagtggaat gtctccacgg
      781 agaacacttt atctatcggg aaatagtgcg ggcaacagtg tttaattata tcgagtgcaa
      841 ttacaattgc tggcgacggc accgttggtg tggcggcctc agtccggaac aatttgaaaa
      901 ccagaacctc gcttaggcct gtgtccatat tacgtgggta ggctcacaga gcctcactta
      961 cgactgcgtc catattatgt gggtaggatc aataaagacg ttttatatgt gtctgcttgc
     1021 agccagaaaa tttataccct gcaggtatca taaacatgct tgataactta attggggctc
     1081 cctcttcctt ttatggtgta attgtttgat tgaatacttc cggggatttc agtatgaaat
     1141 gaagtatatt taatatactt tacaaaaagt ttgtattagt ggaataagga caatagctta
     1201 tccaaactgc caagcctctt tattataagt aagatctggc actggaatat tctcaaaagg
     1261 actaacatca tcaccacgac cttcaaccaa ccattttatt gcatttaaat cccagaatag
     1321 ttcagtagtg gtcaatttgg gaaacgcaca atattgtacg gttacgttat cgcaatagag
     1381 tgaactctcg ccctatacgg cgttttcaag gattagtcct taatcggaca accacattaa
     1441 agcataataa ataattaagc tttaataata tactatagat acagttctat tataaaaaat
     1501 actattttag gtgatgcatt aaattagtag aatatttatt cttttataaa agcaccaatg
     1561 aattgtccaa gtaaacatga gataaaatgt gtagaggata actgagtttg gtcactttaa
     1621 ctgtattagt cgcagtctga ctctcgactt taaaaggatg ggaattacct ggactgatag
     1681 tgagaaagct ttccattgaa ataagtcaaa taaaagttga tggcttcagt gctaaagtga
     1741 ggtgcccgct agccgtattc actcgaatcg gaattcaatg tattgcttct gccatttcca
     1801 taatatttca cgggaaaaac aaaatctgaa aaactatact taagtgttgt attgagtcag
     1861 ttgagataca aatacatatt tatcagcaca cgttacgtca aatacaggag gttgtcatgt
     1921 tggtactgaa aggcacgttg gctattgcag ccgtgatgac aatttattgt cttatcgttg
     1981 ttcttatgga acgcttttct gattgatttc atattggcga gagaacaaga aggtgagtag
     2041 aattgtcgcg gaatcctgaa actatcagcc tcaggtatga acaccaaaag gtaggtagag
     2101 aaatctatgg cccccgttac tttattcgca caatgaggta tttttttatt tttacaaaag
     2161 ttcaataaat attataataa aagatatgtt cttggtaaca taaacaaata tttaattaaa
     2221 ataatgcttg gcattgacgt ttatcttata aagtaagaag atcatggaca tttcaaccaa
     2281 aaaagtagag ttctcgatga aattaaaatt ctttgtactt gcactttgtg tacctgcgat
     2341 ctttactaca catgctacca ctaactatcc actttttata ccggacaaca tcagtacgga
     2401 tattagtcta ggatctctga gtggcaaaac aaaagaacgc gtttatcatc ccaaggaagg
     2461 ggggagaaaa attagtcaac tggactggaa atacagtaat gcgactattg ttagaggtgg
     2521 catcgattgg aagctaattc caaaagtgtc tttcggagtt tccggttgga ctactttagg
     2581 taaccagaaa gcaagcatgg ttgataaaga ctggaacaat tccaataccc ctcaggtatg
     2641 gaccgaccag agctggcatc ccaatacgca tctccgtgat gctaacgaat tcgagctgaa
     2701 tcttaaaggt tggttattaa ataatttgga ttatcgactt ggactaatag caggttacca
     2761 agagagtcgt tacagtttta atgcaatggg agggagttat atttatagtg aaaacggggg
     2821 gagcaggaat aaaaaagggg cacatcctag tggtgaaaga acaataggtt acaaacagct
     2881 cttcaaaata ccttatattg gattaactgc taattaccgc catgagaatt ttgagtttgg
     2941 agcagaactg aaatatagtg gttgggttct ttcatctgat acagataaac actatcagac
     3001 tgagacaatt tttaaagatg aaataaaaaa ccaaaattac tgctctgttg ctgcgaatat
     3061 tggatactat gtcaccccca gtgcaaaatt ttatatagaa ggctccagaa attacatttc
     3121 taataaaaaa ggtgatacat ctctttatga gcaaagtacc aatatatctg gcaccattaa
     3181 aaatagtgca agtattgaat atattggttt tctcacttcc gcaggtataa agtatatttt
     3241 ttgaattggt tcaccgagtt gattacgttt tcatggctaa aaattgccgc ccattgtgag
     3301 tcatattcgc cctgactttc cagaaccata cgaactgccc gttgacggac ttcgggggaa
     3361 aaacgtgtat ttttagtcat tctgtttacc tctttctcag ggcgtttagt ctccaggatt
     3421 tccggggcgg ttcaaaaaac tcttcgttga ttactgtgga cttactgttg gcattaccga
     3481 ccctgagacc ggagaaataa gaaccgctta gatcatcgtg gctgttctcg gggtatcaag
     3541 ttacacatgg gcagaggcta cccaaggtct actattatcg cctgaaagcg ctgatggaac
     3601 agtgctatca gggacatgct gatgaaaata cctaaataat cagccagcac tctatctttc
     3661 caaatccaca gcatagcaaa gagagcaaaa gagcctgtaa attcagaaat taaggaaacg
     3721 cctcttggtg tacataatct atcttatgtt aaatgaaatc taatttcttg attatatgct
     3781 ggatcgttcg tgggtggctt tgcccctata tttccagaca cctgttatca ttggcccgct
     3841 gccgcagata ttcccgtggc gagcgataat ccagcgcact atgcggatgc cattcgttat
     3901 aatgctcgaa tgcctctgca aggttctttg ctgccgttaa cccgtcaggt ttgggcatga
     3961 tactgatgta gtcatacttt attgttttca cgaagctctt tgctattctg ttactctccg
     4021 gactccgcac cgccgtgttc ttcggttcaa caaagtccca acatccgggt gaactggcgt
     4081 gtttcattag ctcggtagca tgaaccatta tccgtcagtc acttcactgg agacgccgga
     4141 agctcgttgc cgaagcggcg ttccaccgct cccagcatgt cgccaacggg agagcatcta
     4201 cggtctgatt gaactgttta ttcaaattgt ccaccgtctg tcagtacgct ctgaacgtcg
     4261 gctggttaaa acactgctcg ctgattttca gagggttcac ggtaaaacag cacttttatt
     4321 ccggattgcg gaagcggctc tgaataatcc ggatgggctg gttaaagagg ttgtgtacca
     4381 tctgcatatt gttgaaccag atcgcagcgg taagagaagt tcatcatact tggcgcagtt
     4441 acgcgatgtt tctgctagag gtgatgccgt aaagaacggc agaacattac cggaacaaga
     4501 tagcggtcta cctgcactgg tatcagaccc ggggcttccc cggatgatct ccacggtact
     4561 ttaggtactg tgtctatatt atgtaggtag gatcaattaa ctttgtagtt gaaattcttt
     4621 cattgaaata atgaataaaa aagacaaacc gcattaaaca atgccagaat ataattgcat
     4681 ttttgttccg tttttttaaa tcatagtata caaagaagct tgcatactat atgaaaatat
     4741 aacacccttt actatgatgt ttataaaaca atatatggag tcatgtaggt atgaatttag
     4801 atggtgttag accatactgt agaatagtca ataaaaagaa tgaaagcata tcagatattg
     4861 catttgcaca tataataaaa agggtaaaaa attcatcatg tactcaccca aaagcagcat
     4921 tggttttttt aggagagaaa ggtttttgtg atagcaatga tgttctatct attatgggac
     4981 aacaaatacc aagagtattt aagaacaaga tgttatatga ttatgttttt aaaaatgaaa
     5041 aaagtaaaaa tgattttcta aaaatggctg aatcatggct accacagagt gaaccaatag
     5101 taataaataa tgatgatgac gcattgaatg ctgctgctta tttttctgta aaaaaagcga
     5161 aaataaaaac agtaaacgat actgatttta aagagtataa taaggtttat attcttgggc
     5221 acggtagtcc tggttctcat caattaggcc ttggttcgga acttattgat gtacaaacaa
     5281 tcatttcaag aatgaaagac tgtggtattc taaatgtgaa agatatccgt tttacttcat
     5341 gcggctccgc tgataaagtg gctcctaaaa attttaacaa tgcccctgct gaaagtcttt
     5401 cttgtatcct taactctctg ccttttttta aggaaaaaga atctttgcta gagcagataa
     5461 aaaaacacct tgaaaacgat gagtcattga gtgatggtct aaaaatatcc ggctatcatg
     5521 gatatggagt tcactatggt caagagcttt ttccctactc acattatcgt tcaacttcaa
     5581 ttcctgctga tccggagcat acagtaaaaa gaagctctca gaaaaagact tttattatta
     5641 ataaagaact ggattagtat aaaattttta acctatagtt aattgtttgc atttaagatg
     5701 aagttatttt atctttagta gaaaagtgct tccctatgag ggaaaatcac aaatcatcat
     5761 aatacagaga caaaacgata cgaaaaaata gataaaaaac atcattcttt taccacatta
     5821 ttttccgtga atatgaaaaa taatgttatt actttaatat aagactattt tttgtttttc
     5881 catcactctg ttcaaatttt tccgcatgac ttgtgttttt tgtaatacag ctcgtttttt
     5941 acagctgacc aaaatcatca attaattatg ctaaggaaat aaattatgaa aaccaaaaac
     6001 tttcttcttt tttgtattgc tacaaatatg atttttatcc cctcagcaaa tgctctgaag
     6061 gcagaaggtt ttctcactca acaaacttca ccagacagtt tgtcaatact tccgccgcct
     6121 ccggcagagg attcagtagt atttcaggct gacaaagctc attatgaatt cggccgctcg
     6181 ctccgggatg ctaatcgtgt acgtctcgct agcgaagatg catactacga gaattttggt
     6241 cttgcatttt cagatgctta tggcatggat atttcaaggg aaaatacccc aatcttatat
     6301 cagttgttaa cacaagtact acaggatagc catgattacg ccgtgcgtaa cgccaaagaa
     6361 tattataaaa gagttcgtcc attcgttatt tataaagacg caacctgtac acctgataaa
     6421 gatgagaaaa tggctatcac tggctcttat ccctctggtc atgcatcctt tggttgggca
     6481 gtagcactga tacttgcgga gattaatcct caacgtaaag cggaaatact tcgacgtgga
     6541 tatgagtttg gagaaagtcg ggtcatctgc ggtgcgcatt ggcaaagcga tgtagaggct
     6601 gggcgtttaa tgggagcatc ggttgttgca gtacttcata atacacctga atttaccaaa
     6661 agccttagcg aagccaaaaa agagtttgaa gaattaaata ctcctaccaa tgaactgacc
     6721 ccataaagct ggacagcctg tatcaggcta tggagggccc atagacaaat ctaccctata
     6781 tgagcatagg aggagtctat gggcacacca cgttttaccc ctgaatttaa gggattactg
     6841 gaaaggctgg gacatatccc tccggcagaa gcagaaaaag cttattatgc tgccatcgga
     6901 aacgatgatc tggcaacctg agttcacaga taaaacattc tctaggaaac tcggggcggt
     6961 tccgttcacc acatgcaatg tggtgttgca ggggaacggt ctgcaccatc ccctatgtcg
     7021 atcaatataa cagaaatgac aacttcagat tcagggcaca acctaaatat attttaggtc
     7081 acctctcaaa tcgtttgcct gataccgctc cattttttaa taaaaaaatc aatcattttt
     7141 gaatctttgc ttttcattgc attgtcaagc atagtgtccc cactatttgc tttttgaaat
     7201 tttttgttta catcaaccaa tccattattg ataaaatatt ctagcacttt atagttggca
     7261 ccatctttac taagtaagta ttcaacatca tacagattac aatatctatt gtttttcatt
     7321 ttctccatct ctgcgacatc ttgttttgta aaattaaatt tagatagtaa ataaagtgct
     7381 atatcagctt ttttattgcc taaagaataa gcaagggcct gatgagccat ttttttatca
     7441 gtcactttat tgttaatctc ttcataatta gatgctttaa tcgcatcctc caaagatatg
     7501 tctctgagtt ttattttttt gaagttatct gtacttagca ttcttggtat atgatactca
     7561 agctgaaaca caaagtttat aattctatcc agactaacag gatcaatatt tttgttataa
     7621 caaaactctc tgaatccttg atctttactc tttcttataa aatcaataag atgcaaccca
     7681 attccaagcc tcatatcttt agtgttaaat attggaacat cagttttccc ctgattacca
     7741 tgcacatacc tgctaacttc atccacaacc tctttaaagt tatctaagaa aaatgattgg
     7801 tgctcatggt aaaaaacagg tggaatagca ttatcaaagt gatcggttaa tgtcatatat
     7861 ctatatggag agtcatgatc aatgatatac gcattggcgc caaaatctac agtatgatgt
     7921 gttttattta gtgggagttt ttcttgatga ttggaaattt cgaccccaaa aaaaacaaaa
     7981 tcgtggttag agatttcttt aatatcctca gagtatgtat tcttatgtgt ttgtatattt
     8041 tcagactgaa ttttatcaaa ggatttaata ttaagaatat tatttgacag attagagtta
     8101 ctttgatgtc tgaagttaaa attagaatta ataataatag aaagtaagtg acgttcgtct
     8161 gaagaaagag gatgaacttt ttgtattatc tcagagatat agtgcctgga caccttatcg
     8221 agatgctcaa gtgaccgaaa tgatgaccgt gctccagatg gcgcttcaat agaaagattt
     8281 agtgatttga aactggtccc ttgagaaaac attttgctat aactttgcgc tgctatagac
     8341 ttccttaaaa agtttttcac agtattagca cagtgatcta aatgtttttc acttatgcca
     8401 gaagaagatg agtttgttac gcgtaatata ttttttgtaa tgcttttgtt gctttattcg
     8461 gattcgtttt tccatcaaca agatcaatat tattctgaac attaatatga tttactgctt
     8521 caggtatttt cataaaatat tccccattat gtgcgttttt atctatggtt atttagaata
     8581 tattatctaa cttaatattt tcttgtgtgt attataacga aatgaacata ttttaatatg
     8641 ttgtacaatt tttactctga aatcccctga aattaatcac acctccttta ctgaaaggaa
     8701 ttattgcaac ggtattattt gtttttgtat atatatcttg cgccttgtta tgtagttaat
     8761 cctcagaata tgatagataa aatagagaca cagagcagag cgtgttctaa catcatgata
     8821 acataaaata attagtaaca ataaatgaaa gaatcatggg agctcaacaa aaagagagct
     8881 aaatagctgt gtggaatagt agatcacttt ggcggtataa tagcagatgg tctgcatact
     8941 ttccggtaac aacatgggcc agaagccccg ctccggcata actgcgtgca atgggttttg
     9001 aaggcaccgg tgcctgcacg atatggtcgc accggcgaca ggccagttta gggcgttgtt
     9061 tttcgataac cttaaaggcg ctgctgataa gctccagctg ctctgacaca tcacatccca
     9121 gtggactgag ttcaccacca caggccggac agcattcctc ttccggccgg atgacccggg
     9181 gggcacgggg aagtgaggct ggtaacggtt tacgggctga agactggcgc agggaagatg
     9241 gcagtaccgg gtcatattgc tcacccagtg tttccgccat ttcctcctga agtgcgctga
     9301 ttcgctcctg tgcttcctgt atccaccgtt cggtttttgc gcgaagtttt tctgagcttt
     9361 taccgaactg
//
LOCUS       DAJVPP010000133         9164 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVPP010000133 DAJVPP010000000
VERSION     DAJVPP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.133"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..94
                     /locus_tag="OQM34_003526"
     CDS             <1..94
                     /locus_tag="OQM34_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCS1453814.1"
                     /translation="YVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            153..809
                     /gene="pdxH"
                     /locus_tag="OQM34_003527"
     CDS             153..809
                     /gene="pdxH"
                     /locus_tag="OQM34_003527"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1453815.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            938..2212
                     /gene="tyrS"
                     /locus_tag="OQM34_003528"
     CDS             938..2212
                     /gene="tyrS"
                     /locus_tag="OQM34_003528"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1453816.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRVQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2271..3134
                     /gene="pdxY"
                     /locus_tag="OQM34_003529"
     CDS             2271..3134
                     /gene="pdxY"
                     /locus_tag="OQM34_003529"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1453817.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3178..3783)
                     /gene="gstA"
                     /locus_tag="OQM34_003530"
     CDS             complement(3178..3783)
                     /gene="gstA"
                     /locus_tag="OQM34_003530"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1453818.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(3889..5391)
                     /gene="dtpA"
                     /locus_tag="OQM34_003531"
     CDS             complement(3889..5391)
                     /gene="dtpA"
                     /locus_tag="OQM34_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1453819.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6002..6637)
                     /gene="nth"
                     /locus_tag="OQM34_003532"
     CDS             complement(6002..6637)
                     /gene="nth"
                     /locus_tag="OQM34_003532"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1453820.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6637..7332)
                     /gene="rsxE"
                     /locus_tag="OQM34_003533"
     CDS             complement(6637..7332)
                     /gene="rsxE"
                     /locus_tag="OQM34_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1453821.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7336..7956)
                     /gene="rsxG"
                     /locus_tag="OQM34_003534"
     CDS             complement(7336..7956)
                     /gene="rsxG"
                     /locus_tag="OQM34_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1453822.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(7960..9018)
                     /gene="rsxD"
                     /locus_tag="OQM34_003535"
     CDS             complement(7960..9018)
                     /gene="rsxD"
                     /locus_tag="OQM34_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1453823.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9019..>9164)
                     /locus_tag="OQM34_003536"
     CDS             complement(9019..>9164)
                     /locus_tag="OQM34_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453824.1"
                     /translation="AKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVV
                     NED"
BASE COUNT         2342 a         2292 c         2327 g         2203 t
ORIGIN      
        1 ctatgttttc tggtcgaaag gtgaggaagc gactgtctat aaacgcgacc gcatcgtctt
       61 gaataactgt cagttacaaa atccacagcg ttgagatttt tccaggggcg gcgcacaata
      121 gcgtcaccca ctgacaatcc gtaaagaaaa ccatgtctga taacgacgaa ttgcagcaaa
      181 tcgcgcatct gcgccgtgaa tacaccaaag gcgggttacg ccgccgcgat cttcccgccg
      241 atccattaac cctttttgaa cgttggctct ctcaggcttg tgaagccaaa ctggcggacc
      301 ctaccgcgat ggtggtcgct accgtggatg aacatggtca gccttatcag cgcatcgttt
      361 tactcaaaca ttacgatgaa aaaggcatgg tgttttacac caacctcggt agccgtaaag
      421 cacatcaaat tgaaaataat ccgcgcgtta gcctgctgtt cccgtggcat acccttgaac
      481 gccaggtgat ggtgatcggt aaagcagaac gactttcgac tctcgaagtg atgaaatatt
      541 ttcatagccg cccgcgtgat agccagattg gtgcatgggt ttcgaagcag tccagtcgca
      601 tttctgcccg cggtatcctt gaaagtaaat tcctggagct gaagcagaag tttcaacagg
      661 gcgaagtgcc attgccgagc ttttggggcg gttttcgcgt cagccttgaa cagattgagt
      721 tctggcaggg cggtgagcat cgcctgcatg accgcttttt gtaccagcgt gaaaatgatg
      781 cgtggaagat tgatcgtctt gcaccctgaa aagatgcaaa aatcttgctt taatcgctgg
      841 tactcctgat tctggcactt tattctatgt ctctttcgca tctggcgaaa agtcgtgtac
      901 cggcaaaggt gcagtcgtta tatacatgga gattttgatg gcaagcagta acttgattaa
      961 acaattgcaa gagcgggggc tggtagccca ggtgacggac gaggaagcgt tagcagagcg
     1021 actggcgcaa ggcccgatcg cgctctattg cggcttcgat cctaccgctg acagcttgca
     1081 tttggggcat cttgttccat tgttatgcct gaaacgcttc cagcaggcgg gccacaagcc
     1141 ggttgcgctg gtaggcggcg cgacgggtct gattggcgac ccgagcttca aagctgccga
     1201 gcgtaagctg aacaccgaag aaactgttca ggagtgggtg gacaaaatcc gtaagcaggt
     1261 tgccccgttc ctcgatttcg actgtggaga aaactctgct atcgcggcga acaactatga
     1321 ctggttcggc aatatgaatg tgctgacctt cctgcgcgat attggcaaac acttctccgt
     1381 taaccagatg atcaacaaag aagcggttaa gcagcgtctc aaccgtgaag atcaggggat
     1441 ttcgttcact gagttttcct acaacctgtt gcagggttat gacttcgcct gtctgaacaa
     1501 acagtacgat gtggtgctgc aaattggtgg ttctgaccag tggggtaaca tcacttctgg
     1561 tatcgacctg acccgtcgtc tgcatcagaa tcaggtgttt ggcctgaccg ttccgctgat
     1621 caccaaagca gacggcacca aatttggtaa aactgaaggc ggcgcagtct ggttggatcc
     1681 gaagaaaacc agcccgtaca aattctacca gttctggatc aacactgcgg atgccgacgt
     1741 ttaccgcttc ctgaagttct tcacctttat gagcattgaa gagatcaacg ccctagaaga
     1801 agaagataaa aacagcggta aagcaccgcg cgtccagtat gtactggcgg agcaggtgac
     1861 tcgtctggtt cacggtgaag aaggtttaca ggcggcaaaa cgtattaccg aatgcctgtt
     1921 cagcggttct ttgagtgcgc tgagtgaagc ggacttcgaa cagctggcgc aggacggcgt
     1981 accgatggtt gagatggaaa agggcgcaga cctgatgcag gcgctggtcg attctgaact
     2041 gcaaccttcc cgtggtcagg cgcgtaaaac tatcgcctcc aatgccatca ccattaacgg
     2101 tgaaaaacag tccgatcctg aatacttctt taaagaagaa gatcgtctgt ttggtcgttt
     2161 taccttactg cgtcgcggca aaaagaatta ctgtctgatt tgctggaaat aatgcattaa
     2221 gtggaaaggg ggagtgagaa atcactcccc ctggttttta tacagggaac atgatgaaaa
     2281 atattctcgc tatccagtct cacgttgttt atggtcatgc gggtaacagt gcggcagagt
     2341 ttccgatgcg ccgcctgggc gcgaacgtct ggccgctgaa caccgttcaa ttttctaatc
     2401 acacccaata cggcaaatgg actggctgcg tgatgccgcc cagccattta accgaaattg
     2461 tgcaaggcat tgccgccatt gataaattac acacctgtga tgccgtatta agtggctatc
     2521 tgggatcggc ggagcagggt gaacatatcc tcggtatcgt ccgtcaggtg aaagcagcga
     2581 atccgcaggc gaaatatttt tgcgatccgg taatgggtca tccggaaaaa ggctgtatcg
     2641 ttgcaccggg tgtcgcagag tttcatgtgc ggcacggttt gcctgccagc gatatcattg
     2701 cgccgaatct ggttgagctg gaaatactct gtgagcatgc ggtaaataac gtcgaagaag
     2761 cggttctggc agcgcgcgaa ctcattgagc aagggccaca aattgtgttg gttaaacacc
     2821 tggcgcgagc tggctacagc cgtgaccgtt ttgaaatgct gctggtcacc gccgatgaag
     2881 tctggcatat cagccgtcca ctggtggatt ttggtatgcg ccagccggta ggtgttggtg
     2941 atgtgacgag cggtttactg ctggtgaaac tgcttcaggg ggcaacgctg caggaggccc
     3001 tggaacatgt gaccgctgca gtctacgaaa tcatggtgac caccaaagca atgcaggaat
     3061 atgagctgca agtggtggct gctcaggatc gtattgccaa accagaacat tacttcagcg
     3121 caacaaagct ctgaaatatc tcatttcggc ccacaacgac ctgtgggccg atggctatta
     3181 ctttaagcct tccgctgaca gcgcgtcttg tacttccgga cgttcagcca tacgttgcat
     3241 aaatgccgca atgtgctcta acccttccag attcagtttc accgcgtatg cccagcgcag
     3301 aaccgtaaac agataggcat cagcaattgt aaatctttgc ccgcagatcc agtgctcatc
     3361 cttcagtgcc tcgttcacat attgcagctt cttatccagc tgcgcgcgaa ctgtcggttt
     3421 gtactcttcc ggtgtatctg ggcgaaacag aggtgtgaaa cctttatgca gctcggtggc
     3481 gatgtaattc agccattcga tggttttata gcgggaaata ctgtttaccg gtgccagcaa
     3541 ctggcggtcg gggacgctgt cggcaagata ctgcataatc gcaacgcctt ccgtcagcaa
     3601 agtaccatcg tccagcagca atgcaggcac ctgcccctta gggttaacgg aaaagtaatc
     3661 gtcaccgttt tcgagacgtt ttttcattaa atccacactg acgagcgtaa aatcctttcc
     3721 gctctcacgc agggtgatat gggaagcgag agagcaggcg cccggtttgt agaacaattt
     3781 catcggtaac tccttttgtc tgtggtttaa agtatggtag tgcgttgctg aacaaaaaaa
     3841 aagccgctaa cgaaaagtta gcggcttgta aaagagtttc cctgaatttt acgctacagt
     3901 agctttcgcc gctttgtctg cagcgtcatc ctgcgtcatg cggtgcagtt taggcgcggt
     3961 cagcagcatc agtactgcaa taacggcagt agcgacacca atctgcaaga atacgcgacc
     4021 atagacttcc agtgacatca gcgggtcggt aacgttatcc ggcacagcca tcatacccgc
     4081 aacataacca ccaatcaggt ttgcaccggc agtggtcagg aaccagctac ccataatgaa
     4141 gcccatcaga cgctgcggaa cgagttgtgc aaccattgcc agacccagac cagagatcat
     4201 cagttccccg atgctctgca ggccatagct tgcgaccagc cagctgacag acacgatacc
     4261 tgcgtcagac gcgaatttcg cacccagcgg cagaatcagg aacgcaccag aacacatcac
     4321 catgccgatt gcaaacttgg ttggcatcgg cagggtatca cccatcttgt tatagatagc
     4381 ggccagaatc ggactaccga tgatgatcca gaacgggttc agtgcctgat actgctcagg
     4441 ttctacggcc agacccagaa tggagtgctc aacgttacga atcgcaaaga agttcagtga
     4501 cgttggcatc tggctgtaca gcacgaagaa gatgattgct tcgagcatca ggatgaaggc
     4561 aacgatcatt ttacgacgcg cagcaccttt catcgcgaaa gcttctttac cgaagataac
     4621 cacaataccg aaggcaacaa cgcccagcgc catacgcgca acttcctgat tgtgcagtag
     4681 ccaggtggca acagcgatca gcgccacaac accaataatg gtcagcaaca ggttacggta
     4741 gttgactggt tcaaagtctg gttttgaacc gtactgttta acccagcgtt ggcagaaggc
     4801 gaagttaacg atagtgatca gcaggcctac aacgctcaac gcaaacgcaa cactccagcc
     4861 gtatttcgcg gccagccacg gggtggctat catagagaag aaagagccga tgttgacgga
     4921 catgtagtac atggtgaatg caccgtccag acgtgggtcg tttttctcat agcatgtaga
     4981 aagcagagaa gacgggttag ctttaaacag gccgttaccg accgcaatag ccgccatacc
     5041 catataaaca ataccggcgt cgtgaccaga ccaggcaacc agagcataac caatcgccag
     5101 cacaatagcg ccgagcataa ttacgcgttt agtacccagt accttgtcac ctaaccagcc
     5161 gccgatagcg accagaccat aaaccagggc actaaaggaa gagaaaaggg tgattgaatc
     5221 cgcttcagac atacccagtt gtttaaccag gtaaacagcc ataattcctt gtaggccgta
     5281 ataaccaaaa cgttcccata actcaatcga gaagatgaga tagaacgcct tcggttgttt
     5341 gaaagcgttc aaactgacgc tttcagttgg tttttggttt gcagtggaca cttttacctc
     5401 tttttttaca tcccatatta acgggggtgc tttctcgcgt tatgtctaaa aaacatacgc
     5461 gcaattgtta tagtgggagg ggaaacggca ggtaatgttc actatcttgc actttcagac
     5521 aagtcctttg taataatctg ttacatataa ctgaggcggg gaattcaacc attccgggaa
     5581 atgttatcca gcgttaaatt tttcaaaaaa cgtaacggca gattttttca ctgcattgag
     5641 agggggatga tggcttgacg gcgatatgtt ctgctattta gcgtttttcc tagtgagata
     5701 gtccagtttc tgaaaaatag ccagtgtaat gttttgcagg tcaatatttg ttgcgtgtgt
     5761 aaatttaaat cattttaacc tgattcttgc aatgttttta cattgccaca gcatcgtgat
     5821 cttgatcacg aatgaataga aaatttcgct attaaaaaaa caattaacct gcatttttgg
     5881 ttatccgcaa acggattacc tgatgataac gctaagcaat ggcatcattg acgttaaaaa
     5941 gatgttttaa aagaggataa agaaaggtta tcaatggggt aatcggtgtt accccttttc
     6001 ttcagatgtc aactttctct ttgtattcac aaagatcttc aataatacaa gagccacagc
     6061 ggggcttgcg ggcaatgcag gtataacgcc cgtgcaggat caaccaatgg tggcagtcga
     6121 ctttaaactc tgccggaacc actttcagta gcttttcttc tacctgttcg acgtttttcc
     6181 ccggcgcaaa ttgagtacga ttacaaacgc ggaaaatgtg cgtgtcgaca gcaatagtcg
     6241 gccagccgaa tgcagtgttt aatacgacgt tggctgtttt acgacctacg ccgggcaggg
     6301 cttcaagcgc agcacgatct tccggaacct cgccattatg ttgttccagt aatatacggc
     6361 aggttttgat gatattttct gctttgctgt tataaaggcc aatcgttttg atataggttt
     6421 tcaccccttc aacgcctagt tcaagcatcg ctgcaggcgt attcgccacc gggtagagtt
     6481 tcgccgtcgc cttattaaca ctgatatcgg tcgcctgagc ggaaagcagt acggcaatca
     6541 gcaattcaaa aggcgaactg aaattaagct cggtggtggg atgaggattg ttctcacgca
     6601 ggcgagtgag gatctccaga cgttttgctt tgttcatcag acattccctg tttcaccgtt
     6661 tggcaatgca cgttcggcag ctgcttcagc acggcgcttt ttcattcttt catcaatcag
     6721 gtattttcct gccagcatca gtcccaagcc aataaatgca cctggtggca gcatcgccag
     6781 caggaaaggg gagtcggtgt ggaaaatctc cacgcgtaat acttttgccc agctacctaa
     6841 cagcgcatct gcaccgtcaa acaacgtgcc attgccgata atttcgcgta gtgaacccag
     6901 cacgaacatg gcgcaggttg cgcccgtacc aattgaaaag ccgtccagtg ccgaaagcgc
     6961 cggacctttt ttggcggcga aggcttcagc gcggcccaca acgatacagt tagtgacaat
     7021 cagcggaata aaaatcccta atgattgata caggccaaag gcgtaggcgt tgatcagcat
     7081 ctgtacagcg ctgaccaccg aggcgatgat catcacgtaa atgggaatgc ggatctcggc
     7141 tggcgtccag tgacgcagcg tcgaaatggt caggttggtc agcgtcagta ccagcgtagt
     7201 cgcaagtcct aaacccagag cgttagtggc agtggatgtg accgccaata gcggacaaag
     7261 gccgagcagt tggaccagtg cagagttgtt tttccacaac ccctgaacaa taacgtcttt
     7321 aatttcgctc acgctttatt ctccacaggc aggaagttga gaaagttgtg ctggtaacgt
     7381 ctgagcgtac aatccggcgc gttttaccgc attaaccacc gcgcggggag taatcgtcgc
     7441 gccggtgaac tggtcgaaat caccaccatc tttcttcacc gcccagtgcg catcatccgc
     7501 accactgatt ttttttcccg caaaatgggt gagccagtca gaaaggcgca attcgatttt
     7561 atcgccaagc cctggcgttt cgtggtgctc tgtcacgcgc gtgccaagta ccgtgccatt
     7621 aaaatcggct cccaccagca gctgaatcgc accggaatag ccatctggcg cggttgcttc
     7681 cagaacggcg gctaccggtt tgtcatcctg tttggcgatg taaacccgat gctcaccttt
     7741 acctaactct ggcgcagtta ccagatagca actctgtgcg agcgcattgt tatagcgttc
     7801 ggctggcagc acctgatcaa ataacgtctt ttgttgcaga ctggcctgtt cagcaattgt
     7861 cgttttggtc atctggttga tggccgcagt taaccctgta gaacccgctg caaatagtgc
     7921 cagtgtaatg ccgtgttttc ggatagtttt cagcatggtt taccctttgc gatggccgta
     7981 gacgcgcgga cgcgtgtagt aatcgatcag aggaaccgtg atgttcgcca gcaggacggc
     8041 aaaagccacg ccgtcaggat agccgccgaa actgcggatc aaccagacta ataagcccgc
     8101 cagcgcgccg aacatcagcc gaccacgatt ggtcgtagaa gcagtaactg ggtcagtcaa
     8161 aataaagaat gcgccgagca tagttgcacc agacagcaga tgaatttgcg gtgctgccag
     8221 tgtttctggt gagaacaacc agcccaacgt tgcgcataac gccagcgtta ctaagaagct
     8281 gaggggaata tgccagcgaa tcgctttctg ccatagcaac cacacgccac cagccagcca
     8341 ggcgagattt acccattgcc aaccagcgcc cgccagaata ccgctgtaga tcggatattg
     8401 cataatctgt tcaaccgaat gacctgcgcg gacagaggtt ttgaatgtat ccagcggtgt
     8461 cgcctgacta atgccatcaa tacctaagcg tagcgtattc atatcaccac cactggcggt
     8521 atgaccgctg aaaataacct gtatggcgtc gataaaacca gggatgttga ccgcaatttc
     8581 atgtggcggt aaccagctgg tcatctgcac cgggaaggag atcagtaaga ccacataacc
     8641 aatcattgcc ggattaaacg ggttttgccc cagaccgcca tacaactgtt tagcgatgat
     8701 caccgcaaac accgtaccca gtacgaccat ccaccatggc gctagggggg gaatacttac
     8761 cgccagcaat aagcctgtca gcaatgctga gttatctttc aacgttgcgg ctaccgactg
     8821 cttgcgtagt ttgagtacga gagcttcggc taacagagca ctgaccgacg ccaacaggat
     8881 ctgaacgaga gtaccccaac caaaaaacca cagttgcgct gcgattcctg gcacggctgc
     8941 gagcaacacc aacagcataa tgcgcgatgt ctggcgctgg ttatgggtat aaggggagct
     9001 agctattctg aataccattt agtcctcgtt tacaaccttt tgctgggcgg cttttttggc
     9061 ctgaacgcgg gcaatagccg cggcaactgc ggctttgcgc ggatcgacct gttcttctgg
     9121 ttccgcatta gcctgttgct gttccagctt gcgtgctttg gcgc
//
LOCUS       DAJVPP010000134         9019 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVPP010000134 DAJVPP010000000
VERSION     DAJVPP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.134"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..894)
                     /locus_tag="OQM34_003537"
     CDS             complement(<1..894)
                     /locus_tag="OQM34_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCS1453825.1"
                     /translation="MNRRRKLLIPLLFCSAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            954..1232
                     /locus_tag="OQM34_003538"
     CDS             954..1232
                     /locus_tag="OQM34_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453826.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OQM34_003539"
     CDS             complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OQM34_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1453827.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OQM34_003540"
     CDS             complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OQM34_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1453828.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3386..3718
                     /gene="hdeA"
                     /locus_tag="OQM34_003541"
     CDS             3386..3718
                     /gene="hdeA"
                     /locus_tag="OQM34_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1453829.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3834..4160
                     /gene="hdeB"
                     /locus_tag="OQM34_003542"
     CDS             3834..4160
                     /gene="hdeB"
                     /locus_tag="OQM34_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1453830.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4224..4870
                     /locus_tag="OQM34_003543"
                     /pseudo
     CDS             4224..4870
                     /locus_tag="OQM34_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4912..5442)
                     /locus_tag="OQM34_003544"
     CDS             complement(4912..5442)
                     /locus_tag="OQM34_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1453831.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OQM34_003545"
     CDS             complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OQM34_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1453832.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6411..7634)
                     /locus_tag="OQM34_003546"
     CDS             complement(6411..7634)
                     /locus_tag="OQM34_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453833.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8215..>9019)
                     /locus_tag="OQM34_003547"
     CDS             complement(8215..>9019)
                     /locus_tag="OQM34_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1453834.1"
                     /translation="TPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMW
                     MTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYT
                     PIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIG
                     SFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGAL
                     MALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2636 a         1773 c         1890 g         2720 t
ORIGIN      
        1 gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg
       61 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg tcgggtcgga
      121 gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg
      181 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
      241 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
      301 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
      361 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
      421 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
      481 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
      541 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
      601 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
      661 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
      721 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg agagtgtgac
      781 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
      841 gagcatcgcg ctgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
      901 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
      961 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     1021 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     1081 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat caagtcgttt
     1141 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     1201 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     1261 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     1321 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     1381 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     1441 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     1501 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     1561 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     1621 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     1681 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     1741 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     1801 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     1861 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     1921 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     1981 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     2041 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     2101 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     2161 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     2221 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     2281 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     2341 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     2401 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     2461 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     2521 acatcaagtg actatgatca gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     2581 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     2641 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     2701 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     2761 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     2821 atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     2881 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     2941 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     3001 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     3061 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     3121 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     3181 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     3241 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     3301 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     3361 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     3421 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     3481 accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     3541 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     3601 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     3661 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     3721 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     3781 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     3841 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     3901 acgcacaatc tgcgctggca gccaatgaat ctgctaaaga tatgacctgc caggaattta
     3961 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
     4021 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     4081 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     4141 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     4201 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
     4261 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     4321 gcacacatgt attaattggc atgggaagtg ccctgtttat gattgtttcg aaatatggtt
     4381 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     4441 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     4501 taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg ttattggcag
     4561 cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc tggaagtctt
     4621 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
     4681 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
     4741 ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
     4801 aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
     4861 aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa ttcacaccag
     4921 ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga ggatcatcat
     4981 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
     5041 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
     5101 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
     5161 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
     5221 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
     5281 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
     5341 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
     5401 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
     5461 acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt ataataataa
     5521 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
     5581 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
     5641 tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta atcccacagg
     5701 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
     5761 tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg aatggtaccg
     5821 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
     5881 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
     5941 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
     6001 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
     6061 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
     6121 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
     6181 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggtgata
     6241 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag ttcaattccg
     6301 ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt gttttaaaat
     6361 tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata ttatggtatt
     6421 atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc tatttccacc
     6481 ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg gtatgaaatc
     6541 ttttcgggtt gaagtgtctc gtaatgaaat gctatgaatt catttttaga cattgttgcc
     6601 catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg tttataacat
     6661 atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt cactaccttt
     6721 gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa agagttgtca
     6781 ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg ttgttcatat
     6841 ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa taaatttgcg
     6901 tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt taggactagc
     6961 ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc aagggcttcc
     7021 atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa gtacaagctt
     7081 tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt tttattatcg
     7141 gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag aattttagtt
     7201 atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac agaatcctta
     7261 tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg ttgcgtaagt
     7321 ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac cacaactttt
     7381 gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg ttggagttgt
     7441 tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt ggaattaatg
     7501 ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt tttaacatta
     7561 tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctggggta
     7621 aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt ttttaaattg
     7681 gataattaga acaggaatgg attttcttct acacacaata aatataatgc gttatatctt
     7741 cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt gtgtataaaa
     7801 aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa cgctcatttt
     7861 aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg ttttagatgt
     7921 gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga ctgaaatctg
     7981 ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga taaaatgtca
     8041 gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt
     8101 ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta tgattttcta
     8161 cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat ctatttaaaa
     8221 tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg tcaggaatgc
     8281 agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca ggcttgcgcc
     8341 ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg tcatcagaac
     8401 agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga caacggcgct
     8461 taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg ctgagggaat
     8521 aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca gtacatcttt
     8581 aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta ctacagcttt
     8641 ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac tacaacaact
     8701 cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac aacaatttgc
     8761 tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca ggataatact
     8821 ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt tccaaccaaa
     8881 ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa ataaaaaagt
     8941 gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg gtattgtcga
     9001 gcaactacag aacggggta
//
LOCUS       DAJVPP010000135         9002 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVPP010000135 DAJVPP010000000
VERSION     DAJVPP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.135"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OQM34_003548"
     CDS             complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OQM34_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1453835.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OQM34_003549"
     CDS             complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OQM34_003549"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1453836.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OQM34_003550"
     CDS             complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OQM34_003550"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1453837.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3782..4291)
                     /gene="def"
                     /locus_tag="OQM34_003551"
     CDS             complement(3782..4291)
                     /gene="def"
                     /locus_tag="OQM34_003551"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1453838.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4439..5563
                     /gene="dprA"
                     /locus_tag="OQM34_003552"
     CDS             4439..5563
                     /gene="dprA"
                     /locus_tag="OQM34_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1453839.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5535..6008
                     /locus_tag="OQM34_003553"
     CDS             5535..6008
                     /locus_tag="OQM34_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1453840.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6037..6579
                     /locus_tag="OQM34_003554"
     CDS             6037..6579
                     /locus_tag="OQM34_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCS1453841.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6584..7156
                     /gene="tsaC"
                     /locus_tag="OQM34_003555"
     CDS             6584..7156
                     /gene="tsaC"
                     /locus_tag="OQM34_003555"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1453842.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDVLTGELFRQG"
     gene            7161..7979
                     /gene="aroE"
                     /locus_tag="OQM34_003556"
     CDS             7161..7979
                     /gene="aroE"
                     /locus_tag="OQM34_003556"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1453843.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7976..8233
                     /locus_tag="OQM34_003557"
     CDS             7976..8233
                     /locus_tag="OQM34_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1453844.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8209..8763)
                     /locus_tag="OQM34_003558"
     CDS             complement(8209..8763)
                     /locus_tag="OQM34_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1453845.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2249 a         2186 c         2248 g         2319 t
ORIGIN      
        1 ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa agggaaataa
       61 taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg gaagagtctt
      121 tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc gccttgctca
      181 atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc tccaataatc
      241 gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga ggtgctttca
      301 tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga ggaaacacca
      361 acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa
      421 attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg ctggatcaat
      481 accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat attggcctcg
      541 tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc ggccagcagt
      601 tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc cgccagttcg
      661 gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc cagacgacgc
      721 gccagccctg caccgatatt accgccacca accagcatga tccgtttata cggtttttcc
      781 agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat aaagaacact
      841 tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc gtggcggaaa
      901 attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga aagtgcatta
      961 ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt accctcagcg
     1021 aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt atcgataacc
     1081 aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa tagcttatcg
     1141 gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt gttgaaaagt
     1201 gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac agcaaccagc
     1261 atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc
     1321 acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt ggtatcgaca
     1381 acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc aacctggccg
     1441 gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt tttgattagc
     1501 ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt acccggttgc
     1561 tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa ggctttaatc
     1621 tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt tccaccggat
     1681 tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag ttccgggata
     1741 tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc cgttgctgaa
     1801 caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg ggaagggtaa
     1861 cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt gtcgtaaatg
     1921 cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg tgccacctca
     1981 aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg ttcaccgttt
     2041 tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt aacccatccg
     2101 tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac agcatcaggg
     2161 taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag ccagctgtcg
     2221 cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt attggcttcg
     2281 gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa ccaggaagga
     2341 tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg ccgacgctgg
     2401 aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat tgcgatagcg
     2461 ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag cagttgatac
     2521 aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg acgggccatt
     2581 aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa gcacaactct
     2641 tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag aatgttgctt
     2701 aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct acgtaaatta
     2761 cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag agtggactat
     2821 cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag gtcttgcgcg
     2881 ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc atcaccagtc
     2941 gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc gttggttgcc
     3001 gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc cagccagctc
     3061 attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga aagtgaccag
     3121 tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt ttcgtcctga
     3181 acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag cccttgtggg
     3241 ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt aatcgggcag
     3301 gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat aatggttaca
     3361 ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc gcgccagcgt
     3421 ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag cacagcttta
     3481 ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc ggcgaccagt
     3541 tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc ttcttcctca
     3601 gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg tcggtctggc
     3661 tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag atgacgcgct
     3721 gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt agttcttatc
     3781 cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt cgttgttgtt
     3841 tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc tcatgctgaa
     3901 tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg cggtcaaggg
     3961 cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg atcgacaggc
     4021 aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg attaacacca
     4081 gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg atatcaacct
     4141 gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata tcatcgacga
     4201 tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga agccgctcgt
     4261 ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt caggagttag
     4321 aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc acttgattga
     4381 cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg agataaggat
     4441 ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg gcgatgatat
     4501 ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg tattgcagca
     4561 aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa agagtatcga
     4621 aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg acagcgaatt
     4681 ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg ttgaaggaga
     4741 actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc attcatggta
     4801 tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag tgacaattac
     4861 gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac aggtaaatgg
     4921 cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc gccatgcccg
     4981 actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc ccctcgatgt
     5041 tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc taagtaaagg
     5101 tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac gttgtgcgct
     5161 tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg gaagcgaagg
     5221 gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag aaattctgga
     5281 aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat tttattcacc
     5341 agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag gagatgaggt
     5401 aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg tagttactca
     5461 actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct atgtccgatt
     5521 gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat attcacactg
     5581 aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac gcaggttttg
     5641 agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat tatcaggaag
     5701 ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt tatacaccgg
     5761 aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt gagcagattc
     5821 aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg ctggataacg
     5881 cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc aacatcccgg
     5941 gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat gaaggtatgc
     6001 tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc actgttcacg
     6061 gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg atccgggaaa
     6121 cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg tcctctgaaa
     6181 tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc cgtatgtggc
     6241 gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag taactaccct
     6301 gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac atgcccccaa
     6361 tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt tcactcctgt
     6421 gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg agaatgccct
     6481 gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa acacttttgt
     6541 gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata ataacctgca
     6601 aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca tcgcctatcc
     6661 aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag tgatgagact
     6721 gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg caaattacga
     6781 gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg aaaccatttt
     6841 ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa caccgcgctg
     6901 gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt tggtggttgc
     6961 tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga gtggattgcc
     7021 accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc cggttgtgcc
     7081 tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgtcctga cgggtgaact
     7141 gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg atagcccaca
     7201 gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa catccctatg
     7261 ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc tttcgtgctg
     7321 gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc agagcggatg
     7381 agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg ttagaagatg
     7441 gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg gaacgtctgt
     7501 cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca tctcgcggcg
     7561 tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg acggtatccc
     7621 gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg ttgggtatgg
     7681 acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt ggcatcagtg
     7741 gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc tatgacatgt
     7801 tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc tcaaagcgta
     7861 atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt ctctggcacg
     7921 gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg tccgcgtgaa
     7981 tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat gtgtatgttt
     8041 tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga gtctggcgta
     8101 tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc atcgctggga
     8161 cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc aaggctgggt
     8221 ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg cggaatagcg
     8281 taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc taccgagata
     8341 cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac caatcatcac
     8401 atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca aaactcgatt
     8461 gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt cgccaatggt
     8521 taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca tactgccatc
     8581 ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa tcacaacgag
     8641 cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac tgctatcgtc
     8701 gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta aaacatcaga
     8761 catacttacc tcagcaataa atgatttact aatgactttg ggggcattat tggccttgtg
     8821 caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa aaagggtgaa
     8881 aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg ggatccctat
     8941 aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg aacaacgaag
     9001 ag
//
LOCUS       DAJVPP010000136         8981 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVPP010000136 DAJVPP010000000
VERSION     DAJVPP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.136"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..92)
                     /gene="pflA"
                     /locus_tag="OQM34_003559"
     CDS             complement(<1..92)
                     /gene="pflA"
                     /locus_tag="OQM34_003559"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="activates pyruvate formate-lyase 1 under anaerobic
                     conditions; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS1453846.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCL"
     gene            complement(284..2566)
                     /gene="pflB"
                     /locus_tag="OQM34_003560"
     CDS             complement(284..2566)
                     /gene="pflB"
                     /locus_tag="OQM34_003560"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1453847.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(2621..3478)
                     /gene="focA"
                     /locus_tag="OQM34_003561"
     CDS             complement(2621..3478)
                     /gene="focA"
                     /locus_tag="OQM34_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1453848.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(3884..5644)
                     /locus_tag="OQM34_003562"
     CDS             complement(3884..5644)
                     /locus_tag="OQM34_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCS1453849.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSKWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5774..6400
                     /locus_tag="OQM34_003563"
                     /pseudo
     CDS             5774..6400
                     /locus_tag="OQM34_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            6599..7687
                     /gene="serC"
                     /locus_tag="OQM34_003564"
     CDS             6599..7687
                     /gene="serC"
                     /locus_tag="OQM34_003564"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1453850.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVSSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            7758..>8981
                     /gene="aroA"
                     /locus_tag="OQM34_003565"
     CDS             7758..>8981
                     /gene="aroA"
                     /locus_tag="OQM34_003565"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1453851.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKC"
BASE COUNT         2280 a         2136 c         2268 g         2297 t
ORIGIN      
        1 aggcagccct ggaaaaaggt gataaagcga atacctgggc cgtctacggt tccacaggat
       61 tcaaaggagt gaatgcgacc aataactgac attgcggtgt ttctccagat gtggcccatc
      121 tgaggccgtg ttggtgcgca gctcgaaggc tacgtcgagt ctgttttggc agtcacctta
      181 aagtatagat agctgacaaa aaaggctctc gtgctaaaaa aggccccact ttcgtggagc
      241 ctttattgta cgctttttac tgtacgattt cagtcaaatc taattacata gattgagtga
      301 aggtacgagt aataacgtcc tgctgctgtt ctttagtcag cgagttgaaa cgtactgcgt
      361 agccagatac acggatggtc agctgcggat atttttccgg gttttccatc gcgtcgagca
      421 gcatttcacg gttcatcacg ttaacgttca ggtgctgacc accttcgatg gatgcttcgt
      481 ggtggaagta accatccatc agaccagcca ggttggtctt acgaacttcg tcgtctttac
      541 ccagtgcgtt cggaacgata gagaaggtgt aggagatacc atctttagcg taagcaaacg
      601 gcagtttagc aacggaagtc agagacgcta cagcaccttt ctggtcacga ccgtgcatcg
      661 ggttagcacc cggtccgaac ggcgcgccag cacgacgacc gtctggggtg ttaccagttt
      721 tcttaccata cacaacgtta gaagtgatgg tcagaacaga ctgagtcggg atagcgtcac
      781 ggtaggtgtg cagtttctga attttcttca tgaaacgttc taccaggtca acagccaggt
      841 catctacacg cggatcattg ttaccaaact gcgggtattc gccttcgatt tcgaagtcga
      901 tagccagacc gtcttcgtca cgaatcggtt taactttcgc atatttgatt gcagacaggg
      961 agtcagcagc aacggacaga ccagcgatac cacacgccat ggtgcggata acgtcacggt
     1021 cgtgcagcgc catcagagag gcttcgtagc tgtacttgtc gtgcatgtag tggatgatgt
     1081 tcagtgcagt gatgtactgt ttagccagcc agtccatgaa gtgatccatg cgctccatca
     1141 cttcatcgta gctcaggaga tcgcctttga tcggttcaga cttcggacca acctgcattt
     1201 tcagtttttc gtcaacgccg ccgttgattg cgtacagcat ggttttcgcc aggtttgcac
     1261 gcgcaccgaa gaactgcatt tgtttaccaa cgatcatcgg gcttacgcag caagcgatag
     1321 cgtagtcatc gttgttgaag tccggacgca tcaggtcatc gttctcatac tgcagagaag
     1381 aggtgtcgat ggacacttta gcggcgaatt tcttgaagtt cagcggcagt ttttcagacc
     1441 acagaatggt catgttcggt tccggagacg gccccatagt gtacagggtg ttcaggaaac
     1501 ggaagctgtt tttggtaacc agggtacgac catcgaggcc cataccaccg atagattcgg
     1561 ttgcccagat tgggtcgcca gagaacagtt catcgtattc cggagtacgc aggaagcgaa
     1621 ccatacgcag tttcatgacc aggtggtcaa ccatttcctg cgcttcttgt tcggtgatct
     1681 tgccagcttt caggtcacgt tcgatgtaca catccaggaa ggtggaggta cgaccgaagg
     1741 acattgcagc accgttctga gacttaacag cagccaggta gccgaagtaa gtccactgga
     1801 tagcttcctg agcgttggta gccggaccag agatgtcgta gccgtatttc gcagccattt
     1861 ctttcatctg acccagagcg cggtgctgtt cagcgatttc ttcgcgcaga cggatagtct
     1921 gttccaggtt tacgccgttt tccagatcag cctgtagaga ggtgaactga gcgtatttgt
     1981 ctttcatcag gtagtcgata ccgtacagcg caacgcgacg gtagtcaccg atgatacggc
     2041 cacggccata agcatctggc agaccggtca gaacaccgga tttacggcaa cgcaggatgt
     2101 ccggagtgta aacgtcgaac acgccctggt tgtgagtttt acggtattca gtgaagattt
     2161 ttttgatcat cgggtccagt tcgcggttgt acgctttgca ggaaccttcg atcattttga
     2221 taccaccgaa cgggataaga gcacgtttca gcggagcttc agtctgcaga ccaacaactt
     2281 tttccaacgc tttattgatg tagccagcgt cgtgagaggt gatggtggaa gcaacagcgg
     2341 tgtcaaaatc aactggcgcg tgagtgcggt tttccagttt aacaccttcc attactttgt
     2401 cccacagggt ggtggtcgct tcagtagcgc cagccaggaa ggactcgtca ccctcgtacg
     2461 gagtgtagtt tttctgaatg aagtcacgga cgtttacttc attctgccag tcacctttgg
     2521 taaaaccttc ccaggctgtg gctaactttt cattaagctc ggacatgtaa cacctacctt
     2581 cttaagtgga ttttttattt actgcgtact tcgacaacca ttaatggtga tcgttttcac
     2641 gcaggtaaat gacccagtat gtcaacccaa ccaacaaacc accgccgata atattaccga
     2701 tcgtaaccgg gatcaggtta tcagtgatga agttcatcac ggtcaggtga gaaaaatttt
     2761 ccggtgcaga accgacagcg gtccagaatt ccggagatgc gaagtcgcgg attacaatac
     2821 ccatcgggat cataaacatg tttgcgatac tgtgctcaaa accgctggca acaaacatcg
     2881 cgaccggcag caccataatg aacgctttgt ccatcaggct gcggccagaa taactcatcc
     2941 atactgccag acataccatc aggtttgcca ggataccaag acagacggcc tcaataaaag
     3001 tatggtgcac tttgtggtcg gcggtttgta ggacgtttag tccccattga ccatttgcgg
     3061 tcatatactc gccggaaagc cacattaaaa gtacaaacag cagtgcgccg accaggttgc
     3121 caaaatagac atttagccag tttttagcca actgacacca ggtgatgcgc ccactcgcct
     3181 tagcaacaac aatcaacact gtggaagtaa agagatcggc tccgcagaca acacaaagaa
     3241 tcagccccag agagaagcaa atgccgccaa ccagttttgc catgccgaag ggcattgtgc
     3301 ctgtgccagt ggttgctgtg atatagaaga cgaatgcgat tgagatgaaa acaccggcgg
     3361 taatcgccag atagaaagtc ttaagcggat gtttcgttgc tttatagaca cccgcctctt
     3421 cggccacttt ggccattgca gcaggaagta aaagatcaaa agggttgtca gctttcacac
     3481 taactctctc tttattaagt cggcgacgag atactaacaa agcattatag atgagaaatt
     3541 gatatagatc atatctcgcc tggcttatag gcccgtaact cgcatggttt ttatgcaaat
     3601 acggagtaac tatttgatta tccaaataaa aataaatttt aaaaattaac aaatgagttg
     3661 aattttttcc gcatcctccg ctaaaacagt taattaaaag ggagcatcag gcgaataaag
     3721 taacaatatc gatcgtattt attaaataca aattaccgat atttaacctt ataattacaa
     3781 ttattttatt aatgcaaata tatgtaaagc ggggcattaa aaaaacgccc cgtaatataa
     3841 ctcagactaa tcaaatagcc tacattgcgt aggctatttg attttatttt gcccagaatg
     3901 ctgctttggc gcgctgcagc ttttcgtagg ccttcaacaa cgactgatgt gcagcaaacg
     3961 cgtgcagatc gctatctacc ggttgcaggc cgtaaaacgc cgcttcgccg ctcattgccg
     4021 cactggcggc ttctacggca tctgcgccgt acatgcgaac aaacgcattc agatattgca
     4081 gcggctggcg atcttcttcc tgtgccagta ataacaacgt ttgcaggcag cgataatagt
     4141 tggcgcgttc cggactaaat actgatgagt taaactccat cgtccattcg gtccagacca
     4201 gagcctgttc cagatcgcca ccagccagcg ccagcatggc ttttaattca ccgatacgca
     4261 gggtgtacca accgttatcc gacccggtcg ccagacccaa cagctcacgc acgcgggtaa
     4321 agtcatcaaa atcttcttca tccagttgct cgatgaggtt caggtaatct tctttttccc
     4381 acttgctgcc tggtagcgaa agaatcgttt cacgtaaatg gctgcccata ctgttattcg
     4441 cgagccacag atcttcagcc ggataaatat cggacatgcc aggcacgata atacggcaag
     4501 cataaacgcc cagatgctcg taatcggcaa tataaacttc tttatcttct ttgttgaaga
     4561 tagccatcag cgtagcaaac tcttcttccg tggtgccgga gaaattccag tccacaaacg
     4621 gataatcggc atcctgcttg aacaggtccc aggagattaa accgctggaa tcgataaagt
     4681 gcgtttcgag gttggtatgt tcagcgactt cttcatcatc gaaggttggc ggagtaaaca
     4741 catccaaatc tttcaggcca cgaccttgca gcagctcggt cacggtacgt tccagtgcta
     4801 cgccaaaatc aggatgcgca ccgaaagagg caaagcaggt gccgtttgca ggattaaaca
     4861 gtaccacgca aatcaccgga tactggccgc caagggaacc atcatatgcg aagatcggga
     4921 aaccttctgt ttccagtgtt tcgatcgctt caaccaccgc tgggtaacgc gccagcacgt
     4981 ctgccgggat ttccggcaag ctgatgcttt cagcaataat gcggtttttc acgtagcgtt
     5041 cgaaaacttc ggacaacccc tgaacgcgtg cttcgttgcg ggtattgcca gcggacatac
     5101 cgttagaaac gtacaggtta ccaatgatat tcatcggaat ataaacggtc tgattgtcgg
     5161 actggcgcgt aaacggcaga ccgcaaatac cacgatcttc gttaccggat tgtaggtcaa
     5221 tcagcatact gccggtcaat tcattctccg gatcgtaaaa tgcgcgcaga cggtcatcga
     5281 gcagcccttc tggcacatcg tcattttcgc tcagtgggaa ccatttttcg ttgggataat
     5341 gcacaaatgg accgttggcg atggtttcgc ccagccagaa atcagcaaaa aagtagttgg
     5401 ttgagagacg ctcgaaatat tcaccgagtg cagaagccag cgccgctttc ttggttgcgc
     5461 ctttaccgtt ggtaaaacac agtgcgcact ctttgtcgcg aatatgtaca gaccagacgt
     5521 taggcacggg attcagccag gaggcctctt caatctgaaa gccgaggtct gaaagttttt
     5581 gctggaagcg agcgatggaa tcttccagag cggcatcttt gccggggata aatgtttgcg
     5641 tcatgaaaat cactttagtc gtacggaaag cgcgcaataa tacgggtttt atctcaaagg
     5701 cgctatcacc gccgccatgc cgatgaactg ttgactatgc ttttagcgga taaaccacgt
     5761 taagagcata aaaatgaagg cgttcgatct ccaccgtatg gcatttgata aagtgccttt
     5821 tgatttcctt ggcgaagttg cactacgtag tctttatacc tttgtactgg ttttttgttc
     5881 ctcaaaatga ccggaagacg cggtgtgcga cagatgtcgc tgtttgaagt gttaatcatt
     5941 ctcacgctgg gttcagcggc gggagatgtg gcgttttatg atgatgtgcc gatggtgccg
     6001 gtacttatcg tctttatcac tctggcgttg ttataccgcc tggtaatatg atggcgagct
     6061 ggcctggtcg aaactcaata actccaacat gacggaattt gagttcttta tggagctacg
     6121 attgcgtggc gtggagcagc tggggcaggt acgtctggcg attctcgaaa ccaacgggca
     6181 aatcagtgtc tatttctttg aagatgacaa ggtgaaaccg ggtttactta ttttacccag
     6241 tgattgtacg cagcgttaca aagtggtgcc ggagtcggcg gactatgcct gcattcgttg
     6301 tagtgaaatc attcatatga acgcggggga aaaacaatta tgtccgcgct gtgcaaatcc
     6361 agaatggacg aaggcaagtc gggcaaaacg ggtgacctga cagtaaaaac atcggctttt
     6421 tgctaataat ccgagagatt cttttgtgtg atgcaagcca catttttgcc ctcaacggtt
     6481 ttactcattg cgatgtgtgt cactgaatga taaaaccgat agccacagga ataatgtatt
     6541 acctgtggtc gcaatcgatt gaccgcgggt taatagcaac gcaacgtggt gaggggaaat
     6601 ggctcaaatc ttcaatttta gttctggtcc ggcaatgcta ccggcagagg tgcttaaaca
     6661 ggctcaacag gaactgcgcg actggaacgg tcttggtacg tcggtgatgg aagtgagtca
     6721 ccgtggcaaa gagttcattc aggttgcaga ggaagccgag aaggattttc gcgatcttct
     6781 taatgtctcc tccaactaca aggtattatt ctgccatggc ggtggtcgcg gtcagtttgc
     6841 tgcggtaccg ctgaatattc tcggtgataa aaccaccgca gattatgttg atgccggtta
     6901 ctgggcggca agtgccatta aagaagcgaa aaaatactgc acgcctaatg tctttgacgc
     6961 caaagtgact gttgatggtc tgcgcgcggt taagccaatg cgtgaatggc aactctctga
     7021 taatgcggct tatatgcatt actgcccgaa tgaaaccatc gacggtatcg ccatcgacga
     7081 gacgccagac ttcggcaaag atgtggtggt cgccgccgac ttctcttcaa ccattctttc
     7141 ccgtccgatt gacgtcagcc gttatggtgt aatttacgct ggcgcgcaga aaaatatcgg
     7201 cccggctggc ctgacaatcg tcatcgttcg tgaagatttg ctgggcaaag cgaatatcgc
     7261 gtgtccgtcg attctggatt attccatcct caacgataac ggctccatgt ttaacacgcc
     7321 gccgacattt gcctggtatc tatctggtct ggtctttaaa tggctgaaag cgaacggcgg
     7381 tgtagctgaa atggataaaa tcaatcagca aaaagcagaa ctgctatatg gggtgattga
     7441 taacagcgat ttctaccgca atgacgtggc gaaagctaac cgttcgcgga tgaacgtgcc
     7501 gttccagttg gcggacagtg cgcttgacaa attgttcctt gaagagtctt ttgctgcagg
     7561 ccttcatgca ctgaaaggac accgtgtggt cggcggaatg cgcgcgtcta tttataatgc
     7621 catgccgctg gaaggcgtta aagcgctgac agacttcatg gttgagttcg aacgccgtca
     7681 cggttaatgc cgaaattttg cttaatcccc acagccagcc tgtggggttt ttatttctgt
     7741 tgtagagagt tgagttcatg gaatccctga cgttacaacc catcgctcgt gtcgatggca
     7801 ctattaatct gcccggttcc aagagcgttt ctaaccgcgc tttattgctt gcggcattag
     7861 cacacggcaa aacagtatta accaatctgc tggatagcga cgacgtgcgc catatgctga
     7921 atgcattaac agcgttaggg ttaagctata cgctttcagc cgatcgtacg cgttgcgaaa
     7981 ttatcggtaa cggcggtcca ttacacgcag aaggtgccct ggagttgttc ctcggtaacg
     8041 ccggaacggc aatgcgtccg ctggcggcag ctctttgtct ggatagcaat gatattgtgc
     8101 tgaccggtga gccgcgtatg aaagagcgcc cgattggtca tctggtggat gcgctgcgcc
     8161 tgggcggggc gaagatcact tacctggaac aagaaaatta tccgccgttg cgtttacagg
     8221 gcggctttac tggcggcaac gttgacgttg atggctccgt ttccagccaa ttcctcaccg
     8281 cactgttaat gactgcgcct cttgcgccgg aagatacggt gattcgtatt aaaggcgatc
     8341 tggtttctaa accttatatc gacatcacac tcaatctgat gaagacgttt ggtgttgaaa
     8401 ttgaaaatca gcactatcaa caatttgtcg taaaaggcgg gcagtcttat cagtctccgg
     8461 gtacttattt ggtcgaaggc gatgcatctt cggcttctta cttcctggca gcagcagcaa
     8521 tcaaaggcgg cactgtaaaa gtgaccggta ttggacgtaa cagtatgcag ggtgatattc
     8581 gctttgctga tgtgctggaa aaaatgggcg cgaccatttg ctggggcgat gattatattt
     8641 cctgcacgcg tggtgaactg aacgctattg atatggatat gaaccatatt cctgatgcgg
     8701 cgatgaccat tgccacggcg gcgttatttg caaaaggcac caccacgctg cgcaatatct
     8761 ataactggcg tgttaaagag accgatcgcc tgtttgcgat ggcaacagaa ctgcgtaaag
     8821 tcggcgcgga agtggaagag gggcacgatt acattcgtat cactcctccg gaaaaactga
     8881 actttgccga gatcgcgaca tacaatgatc accggatggc gatgtgtttc tcgctggtgg
     8941 cgttgtcaga tacaccagtg acgattcttg atcccaaatg c
//
LOCUS       DAJVPP010000137         8952 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVPP010000137 DAJVPP010000000
VERSION     DAJVPP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.137"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..788
                     /locus_tag="OQM34_003566"
     CDS             <1..788
                     /locus_tag="OQM34_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1453852.1"
                     /translation="LVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEE
                     RTYLSASVKPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQG
                     FSLAKTGWVAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTL
                     LVVQSSSPAQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFAS
                     FAPIVTGWVVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1040..1957
                     /gene="oxyR"
                     /locus_tag="OQM34_003567"
     CDS             1040..1957
                     /gene="oxyR"
                     /locus_tag="OQM34_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1453853.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1940..3340)
                     /gene="sthA"
                     /locus_tag="OQM34_003568"
     CDS             complement(1940..3340)
                     /gene="sthA"
                     /locus_tag="OQM34_003568"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1453854.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISAVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3661..4311
                     /gene="fabR"
                     /locus_tag="OQM34_003569"
     CDS             3661..4311
                     /gene="fabR"
                     /locus_tag="OQM34_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1453855.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4311..4670
                     /locus_tag="OQM34_003570"
     CDS             4311..4670
                     /locus_tag="OQM34_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1453856.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4710..5810)
                     /gene="trmA"
                     /locus_tag="OQM34_003571"
     CDS             complement(4710..5810)
                     /gene="trmA"
                     /locus_tag="OQM34_003571"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1453857.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5924..6114
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6179..8023
                     /gene="btuB"
                     /locus_tag="OQM34_003572"
     CDS             6179..8023
                     /gene="btuB"
                     /locus_tag="OQM34_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCS1453858.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7968..8825
                     /gene="murI"
                     /locus_tag="OQM34_003573"
     CDS             7968..8825
                     /gene="murI"
                     /locus_tag="OQM34_003573"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1453859.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2036 a         2171 c         2417 g         2328 t
ORIGIN      
        1 ctcttgtatt gatgcagctg gcatggggct ggcggacgat gttcgttatc atcggcgtgg
       61 cagggattct ggttgggatc tgttggtacg cgtggtatcg caaccgggcg cagtttgtcc
      121 tgactgacga agaacggacg tatctctccg cgtcggttaa accgcgtcca cagctgcaat
      181 ttagcgagtg gctggcgctg tttaagcatc ggacaacctg ggggatgatt ttaggtttct
      241 ctggcgtcaa ttataccggt tggctttaca ttgcgtggtt acccggttat ttgcaagccg
      301 aacaaggttt cagtctggcg aaaaccggtt gggtagcggc gattcctttc ctcgcggcgg
      361 cggtcgggat gtgggttaac ggtattgtgg tcgatcggct ggcgaaaaaa ggctacgacc
      421 tggcgaagac gcgtaaaacg gctattgtct gcggtttgat gatgtcggca ttaggcacgt
      481 tgctggtcgt gcaatcttcc tcgccagctc aggcggtagc gtttatctca atggcgctgt
      541 tctgcgtgca tttcgcggga acgtctgcat ggggactggt gcaggtgatg gtgtcagaaa
      601 caaaagtggc ttccatcgcc gggattcaaa actttggcag ttttgttttt gcttcctttg
      661 ctccgatcgt aaccggttgg gtagtggata ccacacactc gtttaatctg gcgctggtta
      721 tcgcggcctg tgtgacgttc accggggcgc tgtgttactt ctttattgtc aaagatcgca
      781 ttgagtaatc gttactatca ggctctcttg ctgtgggcct gtagaataaa aaaaagcgga
      841 tcctggagat ccgcaaaagt tcacgttggc tttagttatt cgagttgaga aactctcgaa
      901 acgggcagtg acttcaaggg ttaaaagagg tgccgctccg tttctgtgag caattatcag
      961 tcagaatgct tgatagggat aatcgttcat tgctattcta cctatcgcca tgaactatcg
     1021 tggcgatgga ggatggataa tgaatattcg tgatcttgag tacctggtgg cattggctga
     1081 acaccgccat tttcggcgtg cggcagattc ctgccacgtt agccagccga cgcttagcgg
     1141 ccaaattcgt aagctggaag atgagctggg cgtgatgttg ctggagcgga ccagccgtaa
     1201 agtgttgttc acccaggcgg gaatgctgct ggtggatcag gcgcgtaccg tgctgcgtga
     1261 ggtgaaagtc cttaaagaga tggcaagcca gcagggtgag acgatgtccg gaccgctgca
     1321 tattggtttg attcctacgg ttggaccata cctgctaccg catattatcc cgatgctgca
     1381 ccagacattt ccaaagctgg aaatgtatct gcatgaagca cagacccacc agttactggc
     1441 gcaactggac agcggcaaac tcgattgcgt gatcctcgcg ctggtgaaag agagcgaagc
     1501 attcattgaa gtgccgttgt ttgatgagcc aatgttgctg gctatctatg aagatcaccc
     1561 gtgggcgaac cgcgaatgcg tactgatggc cgatctggca ggggaaaaac tgctgatgct
     1621 ggaagatggt cactgtttgc gcgatcaggc aatgggtttc tgctttgaag ccggggcgga
     1681 tgaagataca cacttccgcg cgaccagcct ggaaactctg cgcaacatgg tggcggcagg
     1741 tagcgggatc actttattgc cagcgctggc tgtgccgccg gagcgcaaac gcgatggggt
     1801 tgtttatctg ccatgcatta agccggaacc acgccgcact attggcctgg tttatcgtcc
     1861 tggctcaccg ctgcgcagcc gctatgagca gctggcagag gccatccgcg caagaatgga
     1921 tggccatttc gataaagtgt taaaacaggc ggtttaaacc gtttaacgca gctacccgat
     1981 aggcttccgc catcgtcggg tagttaaagg tggtgttgac gaagtactca atagtgttgc
     2041 cgccaccttt ctgttccata atcgcctgac cgatatgaat aatttcggca gcgcgctcgc
     2101 caaagcagtg aatacccaga atctcttttg tttcccgatg gaacaaaatt ttcagcgtgc
     2161 ccacgttcat gccgacgatt tgtgcacgtg ccagatgttt gaactgggcg cggcccactt
     2221 catacggcac tttcatcgcg gtcagctgct gttcggtttt gcccacagcg ctgatttccg
     2281 ggatggtgta aatgccggta gggatatctt caatcagatg tgcggtggct tcgcctttta
     2341 ccagcgcctg cgcggcaatg cgcccctggt cataggccgc tgatgccagg ctcggataac
     2401 caatcacgtc gcccaccgcg taaacgtgcg gctgtgcggt ctgatacatg ctgttgacct
     2461 tcagctgtcc gcgactgtca gtttccagcc caatgttctg taacgccagc gaatcggtat
     2521 taccggtacg accgttggca tagagcaggc agtcagcttt cagtttttta cccgacttca
     2581 gatgcatgat cacaccgtcg tcacagcttt cgatcttctc gtactcttcg ttgtgacgaa
     2641 tcactacgcc actgttccag aagtggtagg agagagaatc tgacatctct tgatcgagaa
     2701 atgccagcag gcgatcgcgg gtgttgatca gatccacttt tacatccata ccgcggaaga
     2761 tcgacgcata ttcacagccg atcactccag caccatagat aagtacatgg cgcggttcgt
     2821 ggtgcatgct aagaattgag tcgctgtcgt aaatgcgtgg atgggtgaaa tcaacatctg
     2881 ttggatgata tggacgagag ccgcaggcaa taacaaattt ttcagcggtt agtgtttcaa
     2941 cgctgccgtc cgggcaatcc agcgccaacg tatgctcgtc aacaaagcga gcgttcccct
     3001 gcaatatttc acagtgatta cgttcgtaaa atccctgacg catgcgcgtt tgttgattaa
     3061 tcacgttatc ggcatggtta aggatatcgg caaaagaaga gcggagcagt cgggaatggt
     3121 cgctgtatag tgggttttga ttgaattcta taatgcggct gacggcgtga cggagagctt
     3181 tcgacgggat ggtgccccag tgggtgcaac cgccgccaac attttgataa cgctcgataa
     3241 ctgcgacgcg cgcaccttgc ttaaccaggc ccattgcagc gccttcgccg ccggggccgg
     3301 aacctattac tatggcatcg taatcgtagg aatgtggcat ggtagggctt acctgttctt
     3361 atacataaaa gcaacagaat ggtaacattc tatcgcgggt aagccaattg atccccgtca
     3421 tttatctggc tatatcctga gcggcctttg ctttgtctgt ttcttacttt tgccctgacg
     3481 ttttattgga tttttatcga cgatactctc cgtttaagcg gcaggtttcc gctgtacgta
     3541 aaagaaccgg ccagtaaata tgttttattg cgttaccgtt cattcacaat actggagcaa
     3601 tccagtatgt tcattctctg gtatagtgcc agcagtactt ttggcaagga ttcagacatc
     3661 gtgatgggcg taagagcgca acaaaaagaa aaaacccgcc gttcgctggt ggaagccgca
     3721 tttagccaat taagtgctga acgcagcttc gccagcctga gtttgcgtga agtggcgcgt
     3781 gaagcgggca ttgctcccac ctctttttat cggcatttcc gcgacgtaga cgaactgggt
     3841 ctgaccatgg ttgatgagag cggtttaatg ctacgccaac tcatgcgcca ggcgcgtcag
     3901 cgtatcgcca aaggcgggag tgtgatccgt acctcggtct ccacatttat ggagttcatc
     3961 ggtaataatc ctaacgcctt ccggttatta ttgcgggaac gctccggcac ctccgctgcg
     4021 tttcgtgccg ccgttgcgcg tgaaattcag cacttcattg cggaacttgc ggactatctg
     4081 gaactcgaaa accatatgcc gcgtgcgttt actgaagcgc aagccgaagc aatggtgaca
     4141 attgtcttca gtgcgggtgc cgaggcgttg gacgtcggcg tcgaacaacg tcggcaatta
     4201 gaagagcgac tggtactgca actgcgaatg atttcgaaag gggcttatta ctggtatcgc
     4261 cgtgaacaag agaaaaccgc aattattccg ggaaatgtga aggacgagta atgaaacaag
     4321 caaatcaaga tagaggtacg ctgctgctgg cgttggttgc tggtttatcg attaatggta
     4381 ctttcgcagc gctgtttagc tccattgtgc cattttctgt attcccgatt atttccctgg
     4441 tgctgacggt ttactgcctg catcaacgtt atcttaatcg caccatgtcg gtaggcttgc
     4501 cgggtctggc agctgcctgt tttattctcg gcgtactgct gtacagcacg gtagttcgtg
     4561 cggaatatcc ggatatcggc tctaacttct tcccggcagt actctccgtc attatggtgt
     4621 tctggattgg cgcgaagatg cgtaaccgta agcaggaagt tgctgagtaa tcggtatcat
     4681 gccggatgct ggcgcatccg gcatgggttt tacttcgcgg tcagtaatac gccgcactcc
     4741 atatggtgcg tgtaggggaa ctgatcaaac agagccagac gttcgacctt gtgcgtctgg
     4801 cttaatgttt ccagattttt gcataacgtt tccggattgc aggagatata cagaatacgt
     4861 ggatacgcct gcaccatttt ctcggtttca ctgtccagac cgctgcgcgg tgggtcgaca
     4921 aaaatggttt cgaactgata gctctttaag tcgatccctt gcaggcggtt aaactcgcgc
     4981 acaccattca tcgcctgagt aaattcttct gccgccatac gaataatttg tacgttatca
     5041 atatggttag cggcaatgtt gtattgcgcg gcagcgactg acggtttggc gatttcggtg
     5101 gctaataccc gatcaaaatt gcgcgccagc gctaatgaaa agttaccgtt gccgcagtac
     5161 agctccagta aatcgccttt tgagcctttg gttacgtcca gcgcccattc cagcatctga
     5221 atattcatcg ccgcgttcgg ctgggtaaag ctgttttcta cctgacgata gatcatctct
     5281 ttccctgcga ccggcagacg ctcgtcgatg taatcttgat ccagcgcgat tttggttttc
     5341 gttgcccgac caatcagatg cacattcaga ttctgcgcgc gcagtgcatc gcgcagtgct
     5401 tccgcttcct gacgccactc atcatccagc tttttatggt acagcaggga aaccaccgcc
     5461 tgattactca gcgtggtgag gtaatcaatc tggaacaact tgtggcgcag aacgggatta
     5521 ttacgcacac ccgcaatcat cgccgtcatc aactggttga tgagttcact ggcggcgggg
     5581 aagctatcca cgcggatgcg gcttttggtt tgttgatcga aaatgatgtg atacaggtca
     5641 tcgccatcgt gccagatgcg gaactccgcg cgcatccggt aatgactgac cggcgagcga
     5701 aacacttccg gaaccaggtc agaaaacggt gccatcatac tttgcaaacg taccactttt
     5761 tcggctaact gcgcttcata ctgttctgtt ggaaggtgtt cgggggtcat gatgtatcct
     5821 gaaaaattaa agtacgcggc gattgtaggg attgctcatc agatgtccag atcttgatga
     5881 attcctattt gtgagctacg tctggacagt aacttgttac aacctgtagc atccacttgc
     5941 cggtcctgtg agttaatagg gaatccagtg cgaatctaga gctgacgcgc agcggtaagg
     6001 aaaggtgcga tgattgcgtt atgcggacac tgccatccgg tgggaagtca tcatctctta
     6061 gtatcttaga tacccctcca agcccgaaga cctgccggcc aacgtcgcat ctggttctca
     6121 tcatcgcgta atattgatga aacctgcggc atccttcttc tattgtggat gctttacaat
     6181 gattaaaaaa gcttcgctgc tgacggcgtg ttccgtcacg gcattttccg cttgggcaca
     6241 ggataccagc ccggatactc tcgtcgttac tgctatccgt tttgaacagc cgcgcagcac
     6301 tgtgcttgca ccaaccaccg ttgtgacccg tcaggatatc gaccgctggc agtcgacctc
     6361 ggtcaatgat gtgctgcgcc gtcttccggg cgtcgatatc acccaaaacg gcggttcagg
     6421 tcagctctca tctattttta ttcgcggtac aaatgccagt catgtgctgg tgttaattga
     6481 tggcgtccgc ctgaatctgg cgggggtgag tggttctgcc gaccttagcc agttccctat
     6541 tgcgcttgtc cagcgtattg aatatatccg tggaccgcgc tccgctgttt atggttccga
     6601 tgcaataggc ggggtggtga atatcatcac gacgcgcgat gaacccggaa cggaaatttc
     6661 agcagggtgg ggaagcaata gttatcagaa ctatgatgtc tctacgcagc aacaactggg
     6721 ggataaaaca cgggtaacgc tgttgggcga ttatgcccat actcatggtt atgatgttgt
     6781 tgcctatggt aataccggaa cgcaagcgca gacagataac gatggttttt taagtaaaac
     6841 gctttatggg gcgctggagc ataactttac tgatgcctgg agcggctttg ttcgcggcta
     6901 tggctatgat aaccgtacca attatgacgc gtattattct cccggttcac cgttgctcga
     6961 tacccgtaaa ctctatagcc aaagttggga cgccgggctg cgctataacg gcgaactgat
     7021 taaatcacaa ctcattacca gctatagcca tagcaaagat tacaactacg atccccatta
     7081 tggtcgttat gattcgtcgg cgacgctcga tgagatgaag caatacaccg tccagtgggc
     7141 aaacaatgtc atcgttggtc acggtagtat tggtgcgggt gtcgactggc agaaacagac
     7201 tacgacgccg ggtacaggtt atgttgagga tggatatgat caacgtaata ccggcatcta
     7261 tctgaccggg ctgcaacaag tcggcgattt tacctttgaa ggcgcagcac gcagcgacga
     7321 taactcacag tttggtcgtc atggaacctg gcaaaccagc gccggttggg aattcatcga
     7381 aggttatcgc ttcattgctt cctacgggac atcttataag gcaccaaatc tggggcaact
     7441 gtatggcttc tacggaaatc cgaatctgga cccggagaaa agcaaacagt gggaaggcgc
     7501 gtttgaaggc ttaaccgctg gggtgaactg gcgtatttcc ggatatcgta acgatgtcag
     7561 tgacttgatc gattatgatg atcacaccct gaaatattac aacgaaggga aagcgcggat
     7621 taagggcgtc gaggcgacag ccaattttga taccggacca ctgacgcata ctgtgagtta
     7681 tgattatgtc gatgcgcgca atgcgattac cgacacgccg ttgttacgcc gtgctaaaca
     7741 gcaggtgaaa taccagctcg actggcagtt gtatgacttc gactggggta ttacttatca
     7801 gtatttaggc actcgctatg ataaggatta ctcatcttat ccttatcaaa ccgttaaaat
     7861 gggcggtgtg agcttgtggg atcttgcggt tgcgtatccg gtcacctctc acctgacagt
     7921 tcgtggtaaa atagccaacc tgttcgacaa agattatgag acagtctatg gctaccaaac
     7981 tgcaggacgg gaatacacct tgtctggcag ctacaccttc tgaaccacgt cccaccgtgc
     8041 tggtgtttga ctccggcgtc ggtgggttgt cggtctatga cgagatccgg catctcttac
     8101 cggatctcca ttacatttat gctttcgata acgtcgcttt cccgtatggc gaaaaaagcg
     8161 aagcgtttat tgttgagcga gtggtggcaa ttgtcaccgc ggtgcaagaa cgttatcccc
     8221 ttgcgctggc tgtggtcgct tgcaacaccg ccagtaccgt ttcacttcct gcattacgcg
     8281 aaaagttcga cttcccggtt gttggtgtcg tgccggcgat taaacctgct gcacgtctga
     8341 cggcaaatgg cattgtcgga ttactggcaa cccgcggaac agttaaacgt tcttatactc
     8401 atgagctgat cgcgcgtttc gctaatgaat gccagataga aatgctgggc tcggcagaga
     8461 tggttgagtt ggctgaagcg aagctgcatg gcgaagatgt ttctctggat gcactaaaac
     8521 gtatcctacg cccgtggtta agaatgaaag agccgccaga taccgttgta ttgggttgca
     8581 cccatttccc tctactacaa gaagaactgt tacaagtgct gccagaggga acccggctgg
     8641 tggattctgg cgcagcgatt gctcgccgaa cggcctggtt gttagaacat gaagccccgg
     8701 atgcaaaatc tgccgatgcg aatattgcct tttgtatggc aatgacgcca gaagctgaac
     8761 aattattgcc cgttttacag cgttacggct tcgaaacgct cgaaaaactg gcagttttag
     8821 gctgatttgg ttgaatgttg cgcggtcgga aaattatttt aaatttcctc ttgtcaggcc
     8881 ggaataactc cctataatgc gccaccactg acacggaaca acggcaaaca cgccgccggg
     8941 tcagcggggt tc
//
LOCUS       DAJVPP010000138         8866 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVPP010000138 DAJVPP010000000
VERSION     DAJVPP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.138"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(20..997)
                     /gene="epmA"
                     /locus_tag="OQM34_003574"
     CDS             complement(20..997)
                     /gene="epmA"
                     /locus_tag="OQM34_003574"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1453860.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1322..3130
                     /gene="frdA"
                     /locus_tag="OQM34_003575"
     CDS             1322..3130
                     /gene="frdA"
                     /locus_tag="OQM34_003575"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1453861.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3123..3857
                     /gene="frdB"
                     /locus_tag="OQM34_003576"
     CDS             3123..3857
                     /gene="frdB"
                     /locus_tag="OQM34_003576"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1453862.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3868..4263
                     /gene="frdC"
                     /locus_tag="OQM34_003577"
     CDS             3868..4263
                     /gene="frdC"
                     /locus_tag="OQM34_003577"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1453863.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4274..4633
                     /gene="frdD"
                     /locus_tag="OQM34_003578"
     CDS             4274..4633
                     /gene="frdD"
                     /locus_tag="OQM34_003578"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1453864.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            4696..5829
                     /locus_tag="OQM34_003579"
     CDS             4696..5829
                     /locus_tag="OQM34_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1453865.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            5918..6451
                     /gene="blc"
                     /locus_tag="OQM34_003580"
     CDS             5918..6451
                     /gene="blc"
                     /locus_tag="OQM34_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1453866.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6448..6765)
                     /gene="sugE"
                     /locus_tag="OQM34_003581"
     CDS             complement(6448..6765)
                     /gene="sugE"
                     /locus_tag="OQM34_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1453867.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(6941..7087)
                     /gene="ecnB"
                     /locus_tag="OQM34_003582"
     CDS             complement(6941..7087)
                     /gene="ecnB"
                     /locus_tag="OQM34_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1453868.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7198..7323)
                     /gene="ecnA"
                     /locus_tag="OQM34_003583"
     CDS             complement(7198..7323)
                     /gene="ecnA"
                     /locus_tag="OQM34_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1453869.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7375..7941)
                     /gene="efp"
                     /locus_tag="OQM34_003584"
     CDS             complement(7375..7941)
                     /gene="efp"
                     /locus_tag="OQM34_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1453870.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            7983..>8866
                     /gene="epmB"
                     /locus_tag="OQM34_003585"
     CDS             7983..>8866
                     /gene="epmB"
                     /locus_tag="OQM34_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1453871.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAA"
BASE COUNT         2199 a         2314 c         2295 g         2058 t
ORIGIN      
        1 attcagtaat ttttcagaat tatgcccggt caacgctaaa ggcgatgact tcagccagtg
       61 tctccgcgcc cagcgccaac atcaccagac gatcaacacc taatgccacg ccggaacagt
      121 caggcatacc gactttcaag gcttcaatca gattctggtc aatggggtgc tgcggcaaac
      181 cgcgcgccgc gcgcttacgg ttatcttgtt caaagcgttg ttgctgctca cgggcatccg
      241 ttaattcatg gaaaccattc gccagctcaa tacctttata ataaacctca aagcgttcag
      301 cgacccgatg atcttcggta ctgatttgcg ccagtgatgc ctggctggct ggaaagtggt
      361 acacaaaggt cggtttttct ttaccaatat ttggctctac gccaaaggta aacagcaatt
      421 gtagcaacgt gtcgcggtct tcttcggtat cagcaacatt gctcaaatcc agtttcgctg
      481 cgacttcccg cagttgcgtt ttgtcggcag agagcgggtc aatttccaga taacgcaaga
      541 aagcttgttg ataagaaagg ctttctgctg ccgggcagtc cagcacctgt tgtaagagat
      601 cgtccacctc gttcatcaac cggtacatat catagtgcgg tcgataccac tccagcatag
      661 tgaactcagg gttgtgataa cgccccatct cttcattacg gaagctgcgg cacagctgga
      721 ataccggccc acaaccagca accagcaggc gtttcatatg gtattccggg ctggtcatta
      781 accagagatt catcccctgc gaatgcccgg ggccaacgaa acgtgtctca aacgggacca
      841 aatgaatatc ggttaccgtc gcctggctca tacaaggcgt ctccacctcc agcactccac
      901 gatcggcaaa gaaacgacgg atctccgcca taatcgccgc gcgttttaat aagttaggaa
      961 tggatgcgct cggctgccag gatgccgttt cgctcatagt taaatctcca gtttttgaca
     1021 agggcacgaa gtctactcgc aacgcgacgg cgagacaaat tttacgcagg aatcaaacag
     1081 cggtgggcag tgactaaaaa aagcacgatc tgatggttta gtaattaaat taatcatctt
     1141 cagtgataat ttagccctct tgcgcactaa aaaaatcgat ctcgtcaaat ttcagactta
     1201 tccatcagac tatactgttg tacctataaa ggagcagtgg aatagcgttc gcagaccgta
     1261 actttcaggc acttaccctg aagtacgggg ctgtgggata aaaacaatct ggaggaatgt
     1321 cgtgcaaacc tttcaagccg atcttgccat tgtaggcgcc ggtggcgcgg gattacgtgc
     1381 tgcaattgct gccgcgcagg caaatccaaa tgcaaaaatc gcactaatct caaaagtata
     1441 cccgatgcgt agccataccg ttgctgcaga agggggctcc gccgctgtcg cgcaggatca
     1501 tgacagcttc gaatatcact ttcacgatac agtagcgggt ggcgactggt tgtgtgagca
     1561 ggatgtcgtg gattatttcg tccaccactg cccaaccgaa atgacccaac tggaactgtg
     1621 gggatgccca tggagccgtc gcccggatgg tagcgtcaac gtacgtcgct tcggcggcat
     1681 gaaaatcgag cgcacctggt tcgccgccga taagaccggc ttccatatgc tgcacacgct
     1741 gttccagacc tctctgcaat tcccgcagat ccagcgtttt gacgaacatt tcgtgctgga
     1801 tattctggtt gatgatggtc atgttcgcgg cctggtagca ataaacatga tggaaggcac
     1861 gttggtgcag atccgtgcta acgcggtcgt tatggctacc ggcggtgcgg gtcgcgttta
     1921 tcgttacaac accaacggcg gcatcgttac cggtgacggt atgggtatgg cgctaagcca
     1981 cggcgttccg ctgcgtgaca tggaattcgt tcagtatcac ccaaccggtc tgccaggttc
     2041 cggtatcctg atgaccgaag gctgccgtgg tgaaggcggt attctggtca acaaaaatgg
     2101 ctaccgttat ctgcaagatt acggcatggg cccggaaact ccgctgggcg agccgaaaaa
     2161 caaatatatg gaactgggtc cacgcgacaa agtttctcag gccttctggc acgaatggcg
     2221 taaaggcaac accatctcca cgccgcgtgg tgatgtggtt tatctcgacc tgcgtcacct
     2281 cggcgagaaa aaactgcatg aacgtctgcc gttcatctgc gaactggcga aagcgtacgt
     2341 tggcgtcgat ccggttaaag aaccgattcc ggtacgtccg actgcacact acaccatggg
     2401 cggtatcgaa accgatcaga actgtgaaac ccgcattaaa ggtctgttcg ccgtgggtga
     2461 atgttcctct gttggtctgc acggtgcaaa ccgtctgggc tccaactccc tggcggaact
     2521 ggtggtcttc ggccgtctgg ccggtgaaca agcgacagag cgtgcagcaa ctgccggtaa
     2581 tggcaacgaa gcggcaattg aagcgcaggc agctggcgtt gaacaacgtc tgaaagatct
     2641 agttaaccag gatggcggcg aaaactgggc gaagatccgc gacgaaatgg gcctggcaat
     2701 ggaagaaggt tgcggtatct accgtacgcc ggaactgatg cagaaaacca tcgacaagct
     2761 ggcagagctg caggaacgct tcaagcgcgt gcgcatcacc gacacctcca gcgtgttcaa
     2821 caccgacctg ctctacacca ttgaactgag ccacggtctg aacgttgctg aatgtatggc
     2881 gcactccgca atggcacgta aagagtcccg cggcgcacac cagcgtctgg acgaaggttg
     2941 caccgagcgt gacgacgtca actttctcaa acacaccctc gccttccgcg atgctgatgg
     3001 cacgactcgc ctggagtaca gcgacgtgaa gattactacg ctgccgccag ctaaacgcgt
     3061 ttacggtggc gaagcggatg cagccgataa ggcggaagca gccaataata aggagaaggc
     3121 gaatggctga gatgaaaaac ctgaaaattg aggtggtgcg ctataacccg gaagtcgata
     3181 ccgcaccgca tagcgcattc tatgaagtgc cttatgacgc aactacctca ttactggatg
     3241 cgctgggcta catcaaagac aacctggcac cggacctgag ttaccgctgg tcctgccgta
     3301 tggcgatttg tggttcctgc ggcatgatgg ttaacaacgt gccaaaactg gcatgtaaaa
     3361 ccttcctgcg tgattacacc gacggtatga aggttgaagc gttagctaac ttcccgattg
     3421 aacgcgatct ggtggtcgat atgacccact tcatcgaaag tctggaagcg atcaaaccgt
     3481 acatcatcgg caactcccgc accgcggatc agggtactaa catccagacc ccggcgcaga
     3541 tggcaaagta tcaccagttc tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc
     3601 cgcagtttgg cctgaaccca gagttcatcg gtccggctgc cattacgctg gcgcatcgtt
     3661 ataacgaaga tagccgcgac cacggtaaga aggagcgtat ggcgcagttg aacagccaga
     3721 acggtgtatg gagctgtact ttcgtgggct attgctccga agtctgcccg aaacacgtcg
     3781 atccggctgc ggccattcag cagggcaaag tagaaagttc gaaagacttt cttatcgcga
     3841 ccctgaaacc acgctaagga gtgcaacatg acgactaaac gtaaaccgta tgtacggcca
     3901 atgacgtcca cctggtggaa aaaattgccg ttttatcgct tttacatgct gcgcgaaggc
     3961 acggcggttc cggctgtgtg gttcagcatt gaactgattt tcgggctgtt tgccctgaaa
     4021 aatggcccgg aagcctgggc gggattcgtc gactttttac aaaacccggt tatcgtgatc
     4081 attaacctga tcactctgac ggcagctctg ctgcacacca aaacctggtt tgaactggca
     4141 ccgaaagcgg ccaatatcat tgtaaaagac gaaaaaatgg gaccagagcc aattatcaaa
     4201 agtctctggg cggtaactgt ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg
     4261 taaggagcct gagatgatta atccaaatcc aaagcgttct gacgaaccgg tattctgggg
     4321 cctcttcggg gccggtggta tgtggagcgc aattattgcg ccggtgatga tcctgctggt
     4381 gggtattctg ctgccactgg ggctgtttcc gggtgatgcg ctgagctacg agcgcgttct
     4441 ggcgttcgcg cagagcttca ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct
     4501 gtggtgtggt ttacaccgta tgcaccacgc gatgcacgat ctgaaaatcc acgtgcctgc
     4561 gggcaaatgg gttttctacg gtctggctgc tatcctgaca gttgtcacgc tgattggtgt
     4621 cgttacaatc taacgcatcg ccaatgtaaa tccggcccgc ctatggcggg ccgttttgta
     4681 tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac cgcctcttac
     4741 tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat taccccgctt
     4801 atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg taaaccttat
     4861 tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg
     4921 tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga cgctattgct
     4981 cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact taccgctaaa
     5041 cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg cctgccattg
     5101 caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca aaactggcag
     5161 cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg tttgttcggc
     5221 gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac tcgtgtcttc
     5281 cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga aaagaattac
     5341 gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt agatgctgaa
     5401 gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag caatttaccc
     5461 ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca atctcgctac
     5521 tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg gccggtcaat
     5581 cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg cccggtaaaa
     5641 gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac gggggcgacc
     5701 ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat cgtgatgctg
     5761 gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctgtcagat tctcaacgct
     5821 ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt tccttgctgt
     5881 catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc ctctcgttgc
     5941 cgcagcgaca gctgcatttc tggtcgttgc ctgtagtttt cctacgccgc cgcgtggcgt
     6001 gaccgtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg agattgcccg
     6061 ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca gcctgcgtga
     6121 tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa tgtggcagca
     6181 gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga aagtgtcatt
     6241 ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat accgccatgc
     6301 gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc caaccatttc
     6361 tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg atgtcagtaa
     6421 gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac caataatccc
     6481 caatacgatt aacgccagac tcgccagacg catcgggtta gcggactcac cgagcagcac
     6541 aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat aagccgtccc
     6601 taccggtaac gatttcatcg cccaggcaag tagcgccata ctgactatca tcgccgtcac
     6661 ggtaataaca ctcggcgtca aacggctaaa gccgtgggta tatttcaggc caacggccca
     6721 taccacttcc agcagaccag caataactaa gataatccag gacatatcag gctccggaaa
     6781 tttggggccg tcccctgtga aaagaggcgt ttgcaggtcg tcctgcaaag ctgatggttg
     6841 aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttatga cgatcgcaga
     6901 aaaaacaaac ggcacgacac tgctgtcgta ccgttattgc ttattgctgc gctttcgttg
     6961 ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt
     7021 tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca attgtcttct
     7081 tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc aaattaataa
     7141 tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaagaga agaaaaatta
     7201 gctggcagcg cgagagatgg agttaccgag atgtttgatg tcttcgccga aaccgcaagc
     7261 ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca agaacgataa ggcgtttcat
     7321 catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac cgcattactt
     7381 cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc cgatttgtac
     7441 gaacagcgga actttaacca cagcgccagt agacagggta gccggtttgc cgccagtacc
     7501 tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa cgaagttcgg
     7561 cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg cctgatccag
     7621 cagccattta gcattgtcgc caattgcttt tgcatcagca gacagctgct cgaaagtttc
     7681 gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt tcatatcgac
     7741 aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc gagtaccggt
     7801 cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc
     7861 actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag cacgaaaatc
     7921 gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag ctaagccaca
     7981 aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta acgcaacttg
     8041 ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt gaatatagac gcggacgaaa
     8101 aactgttggc cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta
     8161 tcgatcgcat ggagaaaggc aatccggacg atcctctttt gcgtcaggta cttacctcgc
     8221 aagatgagtt tgtcgtcgcg tccggattct ccaccgaccc actggaagaa cagcacagcg
     8281 tagtgcctgg tttgttgcat aaataccaca accgggcgct tttgctggtc aaaggcggct
     8341 gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa aatcagggca
     8401 acaagcgtaa ctggcagact gcacttgagt atgttgctgc gcatccggaa ctggacgaga
     8461 tgattttctc cggcggcgat ccgctgatgg cgaaagagca cgagctggac tggttgctca
     8521 cacaactgga agccatcccg catatcaaac gtctgcggat tcacagccgt ctgccgattg
     8581 tgatcccggc acgtatcacc gaggcgctgg ttgaacgctt tgcccgttct acgctgcaaa
     8641 tcttgctggt gaatcacatc aatcatgcca atgaggtaga tgaaacgttc cgtcaggcga
     8701 tggctaaatt gcgccgtgtc ggtgtcaccc tgcttaacca gagcgttctg ttacgtggtg
     8761 tgaacgataa cgcacaaacg ttggcaaacc tgagtaatgc gttgttcgat gccggcgtga
     8821 tgccgtatta cctgcatgtg ctagataaag tacagggcgc ggcgca
//
LOCUS       DAJVPP010000139         8788 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVPP010000139 DAJVPP010000000
VERSION     DAJVPP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.139"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(131..2674)
                     /gene="mdoH"
                     /locus_tag="OQM34_003586"
     CDS             complement(131..2674)
                     /gene="mdoH"
                     /locus_tag="OQM34_003586"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1453872.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2667..4202)
                     /gene="mdoG"
                     /locus_tag="OQM34_003587"
     CDS             complement(2667..4202)
                     /gene="mdoG"
                     /locus_tag="OQM34_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1453873.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4596..5753
                     /gene="mdoC"
                     /locus_tag="OQM34_003588"
     CDS             4596..5753
                     /gene="mdoC"
                     /locus_tag="OQM34_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1453874.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5761..7242)
                     /gene="clsC"
                     /locus_tag="OQM34_003589"
                     /pseudo
     CDS             complement(5761..7242)
                     /gene="clsC"
                     /locus_tag="OQM34_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(7184..7717)
                     /gene="ymdB"
                     /locus_tag="OQM34_003590"
     CDS             complement(7184..7717)
                     /gene="ymdB"
                     /locus_tag="OQM34_003590"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1453875.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7812..8123)
                     /locus_tag="OQM34_003591"
     CDS             complement(7812..8123)
                     /locus_tag="OQM34_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1453876.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8244..8576)
                     /gene="csgC"
                     /locus_tag="OQM34_003592"
     CDS             complement(8244..8576)
                     /gene="csgC"
                     /locus_tag="OQM34_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCS1453877.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8635..>8788)
                     /locus_tag="OQM34_003593"
     CDS             complement(8635..>8788)
                     /locus_tag="OQM34_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCS1453878.1"
                     /translation="TLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNN
                     ATAHQY"
BASE COUNT         2199 a         2274 c         2082 g         2233 t
ORIGIN      
        1 tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg acctacaaaa
       61 tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag cgcatcaggc
      121 aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga ttgagcttta
      181 tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag acgcggaaat
      241 gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg cgattcagct
      301 tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt tccagcacct
      361 tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat gacgggttaa
      421 acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca aggaaccgat
      481 cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac agtttccagc
      541 gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac ggtgacagga
      601 tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc cacgccatcc
      661 caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac gcttcgcccc
      721 atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag ccaaggaacg
      781 cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc acggaaaaca
      841 gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct ttcgttcctt
      901 tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc atggtcgaag
      961 caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac aactgccgtg
     1021 gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca gtagagagcg
     1081 cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc aggaacaccg
     1141 cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc aggttaccgt
     1201 ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat tcttcataag
     1261 aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc aacgccgctt
     1321 ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc agcggagcca
     1381 gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc cagtagtgcg
     1441 actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg
     1501 cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc gacgactgaa
     1561 tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa caatcaccgg
     1621 tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc cagcgacggc
     1681 agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg cgatagaaaa
     1741 tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc tctgcgacgc
     1801 agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg gcattcccgg
     1861 tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc acgtcttcgt
     1921 tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca tcgccaactg
     1981 ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag cccatcaacg
     2041 ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc aggataccgg
     2101 tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga ccaaccatat
     2161 ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata taccaggtcg
     2221 cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg gtaccaacgg
     2281 tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga tagcgcggcg
     2341 tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta cgccacgggt
     2401 cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg cgcccttcgt
     2461 cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc agacgcgcct
     2521 ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg gcatccagcg
     2581 cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt gcggcgatgg
     2641 gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta gctccaggtt
     2701 tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtgat tttcttggca
     2761 tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt atagcgcacc
     2821 gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac cggggtatcc
     2881 tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat agtaccgtca
     2941 ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc
     3001 catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat ggtgtattta
     3061 aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc gacgatgtta
     3121 tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt gccccactcc
     3181 cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag atcttcaaag
     3241 cgggagaaat cacgacctcg ttgcaacaga ccaaagcctc gcgggttttc catggagaag
     3301 ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc gccattacca
     3361 gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt tgcaggcgac
     3421 ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag tttgccgact
     3481 ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc tggcataacc
     3541 acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat ggttaaacgt
     3601 ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa ttcttcaccc
     3661 gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac ctgacctgca
     3721 ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt tttatctttg
     3781 ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac cgtgtctttg
     3841 tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg tttgactgcg
     3901 gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg atggtagaac
     3961 tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt aaactggatc
     4021 tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct tttaggcgcc
     4081 tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat actgaaagcc
     4141 cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg cattttcatc
     4201 atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct tatatcgatc
     4261 cgatctattt taatggataa ccagccattc cgacaactga atcccggtat cgttcagcac
     4321 gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc ttctcctaaa
     4381 gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa ccgtcttaca
     4441 gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct tgaagcctgc
     4501 gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata acccgacagg
     4561 gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc gcgcaacgtg
     4621 aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct tttcatattt
     4681 ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca ttgtggctaa
     4741 ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt atatccggat
     4801 acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa gtacgtgtcg
     4861 aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg caatttatta
     4921 tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg tatgacaaat
     4981 ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg ttagtagtca
     5041 tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa aattctgata
     5101 aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc ctcggcatcg
     5161 gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg agtaatggca
     5221 tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc ctcggcgcac
     5281 tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt ggctgtaccc
     5341 tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc agtggcgatg
     5401 cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggcctgtgg atggtgaatg
     5461 tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg acttattttg
     5521 ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt ttcggcgcat
     5581 acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg atattcgtag
     5641 tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg aagtttttgt
     5701 tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt taagccacat
     5761 ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc gcttccagaa
     5821 actggtggcg ggttcttttt tgagaataac ctctttcttt gcatgacgat caacccagtt
     5881 gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc tctgaataaa
     5941 gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca tttcagtatt
     6001 gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt taccatcgat
     6061 gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc gatcgtgtaa
     6121 cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat agcggagcaa
     6181 ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag cggcaagaga
     6241 attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt gcgccacacc
     6301 tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg gtgagcccat
     6361 gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct cccctttcgc
     6421 cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc catcaaccag
     6481 atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca tggcatcgtt
     6541 atgctaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga catccagcac
     6601 ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat cggcaacatc
     6661 ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtggct cttcccctgc
     6721 accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc catcgacagt
     6781 gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat atccaagcgg
     6841 acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg gatgactgtc
     6901 aagcaagcgc aaaatgtcgt caagtccggg cgtattgttg tcgtccagca acaaacggac
     6961 gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc ccgacatatc
     7021 gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg ccagacgata
     7081 ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact gaccgggatg
     7141 ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca tctccttgtt
     7201 gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca aagtatacct
     7261 gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg atttcagccg
     7321 ctgccgcacg agggtaacta taaaccccag tactgattgc aggaaaagcc actgacgtat
     7381 agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa agctggtctt
     7441 cgttttgttc cccaccacgc cagactggcc cgacggtgtg tactacggct ttagcgggaa
     7501 gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc tgttgcctga
     7561 cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc ccatcgacgc
     7621 cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg gccagtttgg
     7681 taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac accttcttgt
     7741 ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag caacgtgagt
     7801 tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg tttatcaaga
     7861 tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat taacgctttg
     7921 ctggagtagg ttttgcccgg aatagaacca ttctgtgggc agttcatttc tacgggtgat
     7981 gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg gatcacgccc
     8041 ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc gagcataaga
     8101 gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca tccttgaggg
     8161 ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct ccagattcat
     8221 cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca ttgttgtgat
     8281 aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc ttccggggaa
     8341 atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga gagggtcttt
     8401 tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat ttgtactctg
     8461 cacagacaag attgagtaag agtaacttca ggaataatgg tgtacatatc cccttgctgg
     8521 gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc attcatattc
     8581 ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga tgtattaata
     8641 ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg aggagttcga
     8701 tgcagtctgg tcaactgcag caccgttgcc accaccgaac tgtttaacag tcatttcaga
     8761 atttttgccg ttccactgat caagagtg
//
LOCUS       DAJVPP010000140         8760 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVPP010000140 DAJVPP010000000
VERSION     DAJVPP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.140"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(65..1558)
                     /gene="pgaB"
                     /locus_tag="OQM34_003594"
     CDS             complement(65..1558)
                     /gene="pgaB"
                     /locus_tag="OQM34_003594"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCS1453879.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYLSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            complement(1567..3990)
                     /gene="pgaA"
                     /locus_tag="OQM34_003595"
                     /pseudo
     CDS             complement(1567..3990)
                     /gene="pgaA"
                     /locus_tag="OQM34_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            4505..5065
                     /locus_tag="OQM34_003596"
     CDS             4505..5065
                     /locus_tag="OQM34_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HCS1453880.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            5008..5397
                     /locus_tag="OQM34_003597"
                     /pseudo
     CDS             5008..5397
                     /locus_tag="OQM34_003597"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            5377..5934
                     /locus_tag="OQM34_003598"
                     /pseudo
     CDS             5377..5934
                     /locus_tag="OQM34_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            6032..7641
                     /locus_tag="OQM34_003599"
                     /pseudo
     CDS             6032..7641
                     /locus_tag="OQM34_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(7742..8233)
                     /locus_tag="OQM34_003600"
     CDS             complement(7742..8233)
                     /locus_tag="OQM34_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HCS1453881.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(8238..8414)
                     /locus_tag="OQM34_003601"
     CDS             complement(8238..8414)
                     /locus_tag="OQM34_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1453882.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         2491 a         1713 c         1850 g         2706 t
ORIGIN      
        1 ctaaagtgaa gtcagttaac gcacgacttt taaagcgggc ccactgttta aattgctccg
       61 ggttttatcg aatttcgctc agactcccgc taaagcctgc ttgctgataa gccgtgatag
      121 ccggtgcact ggcatcttca taatctgaaa gcaaagcatc atcgtggaac aatatgccat
      181 caaaagcagc atgtccggca agatcttcat ataacatgcc aacttgtgct ctgactctgt
      241 catcgaaagg agagagacgg tgatattgtt caggatgaat ttgtgctttt ttctcccctg
      301 ttggtaagta ttttactcgc gttaatgtgg gatctaaatc ccagcttaat accggcatcc
      361 acgcatagat gtttacacct gagcgggtac gtaattgcca ggcaacccga ctaaaaatat
      421 ctgctttcat tggtagcaaa tgatttggaa accagacctc tttgaccagc ccatcaccat
      481 cgggatcagc aaatgcctgc aaatacacgg ttgatatttg catatctttc acccgctgaa
      541 ttagcacatc aatattgcga tccatttgct ggcggttttc gtcataaacg taatcaagat
      601 cgatatgcat tatccgttgt ggtgattttt cctgtacggt aataattttc tgggcaaact
      661 cttttaatga gggattattg gcgattaata ctcgcggaat ggaatccaat tgtgacgcat
      721 ttgccaaacc tgattcaagg gtgaaaaaca tgtcataacc gagttttttt aattcctcta
      781 tcgctatgcc attcgcttcg ccataaggcc aaacaaaaac gtgtggattt acctcagcct
      841 ttgtacgcag gtattccgtc atttttacag catccagacg aattctttcc cggtattctg
      901 ctgcggtttc ataccgtgcg tggtcagtaa aatatgcacg atttacatat acaggcatta
      961 agctgccggt ggaattagcc tgaataccgt agtgagaatt ccatgtatga gaagcgacct
     1021 caacgagccg ggaacgcgca acttctcgca cttgttgcca cgtggcaaaa tattctcgat
     1081 cgaccatctc atcgccaaat tttacttgtt catccactgg cgtatcgacc caactgccga
     1141 cgggggccca tacagcaggc cactggaagg cctgaagaat tgggaagacg cgggtataaa
     1201 aactcaggta gccgtcatca aaagtcagca ctactgcttt ttccggtagc ggttttcctc
     1261 ctcgatgtgc ttcacgaatt tgagcaatac tgaccggttg ataaccgttc tcgcgcagcc
     1321 aggcaaattg ttcacgcagt gctgatgtcc gcactgacat aaaacgctgg tcggcagctt
     1381 cgtcttcaac gttatgccat gaaatcgcca caaaaccatt atgcggccac ggttgctcgg
     1441 cgagtaaaga ttcgcgatcc tgtggcggta taaatgatgt tcttgactgg ctgatgcacg
     1501 cggtgagcat aattatactc accagcatca ggagatattt atttccatta cgtaacatat
     1561 ttatccttaa aatctgaatg tcatatcgaa ttcaacgtat aagttgtgtt ctctgtcacc
     1621 gtcataaggt cgtttttccc agcgtagcgt tgcgccagca tcaatcacgt cattccagct
     1681 aatgcgttgc ccgtagccga gttgggtgac gacatccgtg ccataatgtt tttgccagga
     1741 ggcaccaaca cctgcgctga agatttgctc ccagctattt tcatagcttc gccataacaa
     1801 atgacttgcc tcaaatgccg gaacaatatc gaacgttttt atagggttgt agtatggggt
     1861 atcgtgttct gtattttgtt cgtaatacag actgggtagg aaatcgacaa tcaaatatgg
     1921 tgaagaccag atgcgttcct gaccctcaat tgagacttca tgacgctggt tactgtcgga
     1981 aaaatcagtg aaagcccagg agacaccgta cttacgccgc tcattttgat accagcgaac
     2041 ataagcctga gcactgttgc ctgtaacacc atttttcatt gcccgtaatg gaacgcggtg
     2101 agagaggcgt tccagttgcg aacaaatacg ccagttatca ttaaaatcat accagccaga
     2161 caggcgcgcg ccgggtttat gctcatgatt gaaaaagtgt tcagcgtact ccgcctcgag
     2221 ccagatatta cgtgaccgcc actcaacacc cgccagccag tcgcgaacaa tcccttttcc
     2281 ttcgctaaat tgtccatcgg cataaccgaa tccagcaaaa ccgcgccagt tatccttcag
     2341 cggtggtgaa taaacgatgg tggttaagtc gacatcatgt ttaccactat ccgggccttc
     2401 ggcatcaatt cctgttgaac cagctatacg aagctctgca agattatgta catcaaccgc
     2461 acgttttaaa cgtacaacgc cgggatcttg cggttcacgt tcgacaacat cgtgcgttaa
     2521 gacagctgcc tgctgccatt cttgtaacgt taatgctgtc taggcttgtt caacctccag
     2581 attaatatta cgtggctcga tcacttctgc tttttttaat tcattttctg ctgcacgagg
     2641 ccaaccgcgg gcttgtaaca cactcgcgta atcaatgcgc agtccctgat ttcctggtgc
     2701 gttataagca agctctctgg ctgtcatttc agcctgagga agatcattac tatattttgc
     2761 tacggttgag agaaacgaat gcccctgtaa ctaggtatca ttcgggatgc tcgtaggcgt
     2821 gcccattaac cgaaggaaag gcggcgaagt attaatggta tgttgggtga cagttagcgc
     2881 gcccggataa ttttcactct ctaggtggct gtaaaagaga tccgcaagtt cttcatcgga
     2941 taaatccggg gcaatggtct ccttgtgata aaagagctcg gtcattattg actgtgcttt
     3001 tttcggctga tgatctttga gataagccga tgcaacccaa tattgcccct agggcggaat
     3061 aatttgcccc gtctttttta atcgctgata gtgagaaatg acgtctttat aacgatcgcg
     3121 agttaataac gcgccaagat gatcaacctg aatacgctgg tactgggcag tgcggtctgg
     3181 gttatcgtgc cacagaattt ctaatgcagc gtattgggcg agggcgcgat tggcaatggc
     3241 ataacgttca ctttcactgc gcgtaggcat aaacgacagt ctgaccagtt cggcatgaat
     3301 atcagcgcga atatctggcg ttaaattggc atcgtcaatc gcggcagcaa gttgattatt
     3361 acgtaatgcc tgcacgtatt ctgtgggata ttgttgcgta gatgcatttt caggtaatga
     3421 ctctgtcatc gcccgtaatt catcctgatg acgccccgcc agtttataga tataggcttc
     3481 tgtgagtaaa ttggctttgt ccggtgctcc agagttaagc tgcttaagtt taaccagcgc
     3541 agtatcatag tgacccgcat ctgccagggt taaaatttgt ccccgttgat aatccttatt
     3601 ttgcggctcc agagagagcg ccttttgcca cagtgtaagc gagttttgcc attgttgcag
     3661 gttacgataa gcgacggcga cagctgcata accacgcgct ggtaattgct gatgacggta
     3721 gcggttgtaa acggtaataa cctgtttatc ttgcccggcc cataaggcaa tctgtaacca
     3781 gtcagcaatt tggttattgc tgagtgctga ttttagtgca aaccatgaca aagctggctg
     3841 agtattaccc ttgcgagctt caataatcaa tgcatcatag gcgttattaa cagcactctt
     3901 cgccgcggga ctcgctgcta aaaatgcggc agtaagaagt ttcaaagccc atttggtttt
     3961 cgggcacctt tttctgctac ttgaatacat cctgtattac tccatgtatt gccaaaatct
     4021 ctctctgtat ctaattacag gtaactgaaa agaaagatat ttttgcacct cataatccgt
     4081 tattaaacgc ggaagagaga cgtgaattgt tgatgatgag aagaagaaat gatgagcaga
     4141 gtgtccatat aaaatccttt tctcgcccga aaatccattc caatgatgag gatcttcagg
     4201 aatacggcat aaatcccaat gcctttttca aaataaatta ggattaaaat aattaaatca
     4261 gtaaattccg atgcatgatt tcggattttt cgaaagtcct gatgaaaggc tgcgctacgg
     4321 cactcacgga tttaattgtt attcctaatg tatctatgaa ttaatgtttt ataaggattt
     4381 tctataacca tttataagta ttttcaagcc tggcttgttg caaacaatgt ataaagcact
     4441 taggcaataa taattacatt cagcaactat catcatcggt attgtttgtg ggcggaattt
     4501 catattgaca acagtacagt tcttatatct attaataata gaaagagatc tacaacctac
     4561 agattggtgt agctttatgg aaaaagacta tttgagaatt agtagtactg tattagtgag
     4621 cttattgttt gggcttgctt tggttttggt gaatagttgg tttaatcagc caggcgttga
     4681 ggaagttgtt ccacgatcaa cctatctgat ggtaatgatt gctttatttt ttatcgatac
     4741 tgttgcattt atttttatgc agttgtattt catttatgac cgtaggcaat tttcaaactg
     4801 tatacttagt ttggcttttc tgagttgttt gatttacttt gttaaaaccg tcattatcat
     4861 tcagcaaatt attgaggggc gtttgacaag cagtgttgtc caaaatgaca ttgcaatcta
     4921 ttatttgttt cgtcagatga gtttgtgcat attaatattt ctggcattgg tgaataaagt
     4981 tagtgaaaac acaaaacagc gcaatttatt ttccaaaaaa atgactttgt gcataagttt
     5041 gtttttgttt ttggggggcc gatagttgct cacatacttt ctagtcatta tgagagctac
     5101 aatttacaca ttgccgagtt aaccaatgaa aatggtcagg tggtttggaa agcctcatat
     5161 gttaccataa tgattttcat gtggttaacc ttgctttcgg ttaatctata ctttaatggt
     5221 ttacgctatg atatctggaa tggagtgacg gtgattgctt tttgtgccgt actgtacaat
     5281 atctctctgt tatttatgag caggtatagc gtgtccactt ggtatattag tcgcactatt
     5341 gaagtagtca gtaagttaac cgttatggta atatttatgt gccatatatt cagtgcgcta
     5401 cgagtaacaa agaacattgc acatcgcgat cccttaacca atatatttaa cagaaattat
     5461 ttttttaatg aactgacagt tcaatcagca tcagccaaaa aaacgcctta ttgtgtcatg
     5521 attatggata tcgaccactt caaagaagtc aacgacacct gagggcatcc ggttggcgat
     5581 caggtgataa aaacagtggt gaatatcatt gggaaaagca tacgaccaga tgacctttta
     5641 gcgcgcgtcg gcggcgaaga gtttggcgtc ttgctgacgg acatcgatac tgaacgtgcg
     5701 aaagctttag cggaaaggat tcgggaaaat gttgagcgtt taactggcga taatcctgaa
     5761 tacgctatac cacaaaaagt gacgattagt attggcgctg ttgtgactca ggaaaatgcg
     5821 ttaaacccga atgagatcta tcgactggct gataacgcac tatacgaggc gaaagagacc
     5881 gggcgtaata aggtggttgt gagggatgtg gtgagttttt gtgagtcacc ataaagcggc
     5941 attttagcgg gtgattttgg taagcgctaa atagctattc gcagacttgc gtaacaattt
     6001 tcagaaatat catagtacta tatgatctta aatgcttatc attgatcaat ctgctatcac
     6061 ccgcttttac tggcaattgt actataattt cttaggttgc atcaccctga tgcgtgggga
     6121 aacatgtatg gtattcaaca aaaaaatgtt tgtactcatt ataataccag gcattctggg
     6181 ggtgttacta tcgtttgcta tgtcaatatt tcaaatgaat agagatacga caattaccgc
     6241 cggtattctg ttaaaacagc ttgataatgt tacgcaaatt gtccagcata cgacaaagct
     6301 tacctctatt ctggtgatga agccctgcaa agacattctt gaacagttga ttgctaacgg
     6361 agcattgact ccctatgttc gaacaacggg tcttatagaa aataatttcc aaatatgttc
     6421 ttcagttagc ggtttcaaaa aaatgaatgt taacgatgtt tacggtacga gctttcacaa
     6481 taaaaataaa gaatcacgta tagtatcaat tagcggtaca agttttgtgc caggtaaaac
     6541 agcaatagtg ttccttatgc caatagggaa tgatatgaca gcttttagta ttgtagagtc
     6601 acgatatatc tatgatctaa tggatgttct tgatgatgag aatgatgatt cattttctct
     6661 tcgttttact gaagggcctg caattattag cggtgtaaat aataatgaca ggttatatat
     6721 gttaaaaaga gattttaatt ctgcgatcag tcaggctcgt cttacagtta ctacgccgat
     6781 gatatcgtta tatccctata tcattagcaa tgtgctttat attttgccgt tatctattat
     6841 tctctctttt attctctatt tcttatggca gcactggatg tcacggaaaa tgtcgctggc
     6901 agaagaaatt aaaaaaggaa tgtcatccgg agaattttct gtgcattatc aaccagtgtg
     6961 cgacacgaca accaaagcgt gtctgggggt tgaagcgtta atgcgctggc aacgcgaaga
     7021 tggtaaaaat atatctccgg tagtttttat ccgcgctgca gaagaggaaa atttaataat
     7081 accgttaact aaacatctct ttgaactgat tattcaggat gtacaaagct ggaaagtgaa
     7141 aaaaccgttt caccttggga taaatattgc cgcggaacat cttgcacatc ctgactttgt
     7201 tggtgatgtt ttacatatta aaaatgcaat atctgataaa tttaatattg tccttgagat
     7261 taccgaacgc aatctggttg aagacactga tcttgcatta cagaaaataa atgaattaag
     7321 gatccatggc tgtgaatttg cagtcgatga ttttggcacg ggatattgtt cccttggcct
     7381 tctgcaaaag ttgtctgttg attatcttaa aatagataaa agctttatag acacacttac
     7441 tacagcggaa gacgaaacgc cagttttaga tattattatt aagctgagta atcggctcaa
     7501 tttgataacg attgcagaag gtgtcagtac tcctcaccag cagaatatct tattcataat
     7561 aacgtgacat tagttcaggg atacttatat gaaaaaccaa tgaaagcgac agagttttat
     7621 cagtggtatg taaacagata aataattaaa aatattggaa tggttttgta ttgtcttaaa
     7681 atataaacaa agcaatcaga atgttattaa ctgatgacaa acgccaggat caacaatgct
     7741 gttagtgagt aatacatatg aatcgtggtg tcctgcctgt agagaatatg tagttataat
     7801 cattttgacg cgtaacataa taattaattg aagtgtctgc ttttatgaag aaatcagaag
     7861 tgatattttc aaggatttca tcgctgatgt tatcatcttt ttgtattttt tggattttaa
     7921 ctgtggtgag ataaacggta tcacggaatt tcttgtatgt aaaactcaca tatcggcgga
     7981 tgaagcccat tttttttcca tctaccgtgt atgcgcccgt gagatacatg tttcctgtgt
     8041 tattttcaga aatcatattt aacgaaaaat attgatccat ttttgtatga tcggtgaaat
     8101 taataacatg ggcattacat gatatgtttt gctctgctgg ttcattggaa tgatgaaacg
     8161 caatatggct cagaattgct gcaataaaag cacataaaat tattcccgaa aacagaaaat
     8221 atctttgagt catatgatta ctccgcttta taatagagag agttgcaatt atttggtgat
     8281 tcagagttta acatttgact acaggtgaaa gcataaaacc cgctcatgct atcatttgtt
     8341 gcaatgtaaa tgtaaggatg tattttgcag ttgacttcca ttttctgcaa tttttcaata
     8401 attggtgata gcatgtctac ggaattaaag taattattat catagaaata actgcatcct
     8461 tcctgagtag ggtgttgagc ataatttgag aaagcatcta ttttttttat agtgtgagat
     8521 ttgctttgaa aagccatatc gaaaaaataa gaagacatat aaactatccc ccatgccgca
     8581 aaaaaaaact ataagtaatg tattctttgt ggtaaatata ccttttgcaa cgtaattttt
     8641 atttatattt tcagtgtggg tgccattctc ttcactctca acactttcat tctcattgtg
     8701 attattgtcg agaacagtta tgttgatttc ttcattgatt ttgaagcctt tccgtggcac
//
LOCUS       DAJVPP010000141         8693 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVPP010000141 DAJVPP010000000
VERSION     DAJVPP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.141"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..270)
                     /gene="psiF"
                     /locus_tag="OQM34_003602"
     CDS             complement(<1..270)
                     /gene="psiF"
                     /locus_tag="OQM34_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1453883.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALK"
     gene            complement(389..1804)
                     /gene="phoA"
                     /locus_tag="OQM34_003603"
     CDS             complement(389..1804)
                     /gene="phoA"
                     /locus_tag="OQM34_003603"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1453884.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYATDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLEPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1905..2165)
                     /gene="iraP"
                     /locus_tag="OQM34_003604"
     CDS             complement(1905..2165)
                     /gene="iraP"
                     /locus_tag="OQM34_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1453885.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTALLRDYVKKLLKHPCQ"
     gene            complement(2347..2550)
                     /locus_tag="OQM34_003605"
     CDS             complement(2347..2550)
                     /locus_tag="OQM34_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453886.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2628..3722
                     /gene="ddlA"
                     /locus_tag="OQM34_003606"
     CDS             2628..3722
                     /gene="ddlA"
                     /locus_tag="OQM34_003606"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1453887.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVRDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3746..3958)
                     /locus_tag="OQM34_003607"
     CDS             complement(3746..3958)
                     /locus_tag="OQM34_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1453888.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4218..4526
                     /locus_tag="OQM34_003608"
     CDS             4218..4526
                     /locus_tag="OQM34_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1453889.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4585..5679)
                     /gene="yaiW"
                     /locus_tag="OQM34_003609"
     CDS             complement(4585..5679)
                     /gene="yaiW"
                     /locus_tag="OQM34_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1453890.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5692..6912)
                     /gene="sbmA"
                     /locus_tag="OQM34_003610"
     CDS             complement(5692..6912)
                     /gene="sbmA"
                     /locus_tag="OQM34_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1453891.1"
                     /translation="MFKSFFPKPGTFFLSAFIWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7264..8421
                     /gene="ampH"
                     /locus_tag="OQM34_003611"
     CDS             7264..8421
                     /gene="ampH"
                     /locus_tag="OQM34_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1453892.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8422..>8693)
                     /locus_tag="OQM34_003612"
     CDS             complement(8422..>8693)
                     /locus_tag="OQM34_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1453893.1"
                     /translation="KNDIPYKLISEGNCTGYHLPAKQTRATLLSMIDWNEELRSRIGV
                     MNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         2128 a         2233 c         2090 g         2242 t
ORIGIN      
        1 tttcagagct tgttgcgttg cttgattatt acattcacgc atcttttgct gctgtggcgt
       61 caaacttttt tcaccaggcg cagacttgct gttcttcagg caatcactca tgtaggtctt
      121 acgagcatcc cctttcaacg cctgcgccgt cgcctgctga ttacaggagg tcatacgctg
      181 ttgttgtggg gttaaggttc tctcggcagc gccgacggtg gttaaaaaaa ccagaccaaa
      241 aagcaaggta accagtaatg ttattttcat agcaccatcc ctcttcatgt tttaaccatg
      301 agcgtttgcg cccgtgatct gccattaagt ctggttgcta acagcaaaaa aaccacccgg
      361 cagcgaaaat tcactgccgg gcgcagtttt atttcagccc cagagcggct ttcatggtgt
      421 agaagagatc ggtctggtcg gtcagtccaa caacattggc ggcatgcggg ccatacgccg
      481 caatacgcaa ctgactgccg gtatgttctt gtgaatcctc ttcggaattc ccgtaactca
      541 tcaccatcac tgcgccatct ttagtattta gcgcctgagt gaggcccggc gctttggtat
      601 ccggcgcaac aacctggctg gcgtgggcgt gatcagcggt gactatgacc agcgtgttac
      661 cctccttttt agcgaattcc agcgcccgtt gtacggcttc atcgagatcg accgtctcgc
      721 caatttgccc acaagggttc gcagcgtgat cctgtttatc gattgacgca ccttcaactt
      781 gcaggaaaaa tcctttctca tttttactca acaattcaat ggctttgtcg gtcatctgtg
      841 ccagtgttgg tacactgtca ttacgttgcg gattaggcgt ataggtgact gcgggcttat
      901 cgatattgcc gtggtacgtt gctttcggct ctagccagcg cactggcata ttgccgtcag
      961 caaacaggcc aagcaggggt ttttgctgat tcgcttccgt caccgaattc agtgaggcag
     1021 catcgttcac caactgataa ccacgcgcct gtgcctgttc acgcagcgtt tttccctgcc
     1081 attcaccagc ggttgccgtt tcagcaaagg tttttgcgcc gccgccaagc gtaacgtcgg
     1141 cacgagcgtt aagcagctgt tcggtaatcg atccttttcc gcctttttcc agagcgttac
     1201 ccggacattt ttcactggtc gcgctcggac cgtagcattt gcgcgaagtc acatgtgcca
     1261 ccagcgcagc gggcgtggca tcctgcaact ctgcggtaga aacgttaccg gtcgccagac
     1321 ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt ttcgtgaata tcgacgccca
     1381 gcgcgccgtt ataggttttg acaccggttg accaggcggt tgctgatgca gccgagtcgg
     1441 tggcgtagtc cggtttgcca gtttttttat tcagcgcata gtgagtgtat tgcccggtaa
     1501 gcggtaaggc atcgatacct ttaaaaaagc cgcccgcacc ttcggcataa ttacgtgcgg
     1561 cagtaatttc cgaatccccc atcccatcgc caatcagcaa aataatattt tttgcaggtt
     1621 tatcgctaag agaatcacgc agagcggcgg tctgatcacc cgttaagcgg cgagcaccgc
     1681 cgggtgcagt aatatcgccc tgagcagccc ggttttccag aacaggcatt tctggtgtcc
     1741 gggcttttgt cacaggggta aacagtaacg gtaagagtgc cagtgcaata gtgctttgtt
     1801 tcactttatt ttctccatgt acaaatacat taaaaaataa aaacaaagcg actataagtc
     1861 tcggccgtga caactttatg acagctgttg aaaagattaa ctttttactg acaaggatgc
     1921 ttcaataact tctttacgta atcgcgcagc agcgctgtat cgtcgtcagg aatgctggca
     1981 tcgggcttta cctcgtacag cgccccctct acctgatcaa tcaaccgctg ttggtcattt
     2041 tgcgccatat tgcgaagcat tgcagtgacg ataatctcca aagcttctac ctgcgcacac
     2101 agttctttcg actcttcttc tttttgggca agcttaaata acaactcagc aatgagattt
     2161 ttcatgtctg tatttcctta tccaaagtat ggagaagtta acattatgtt tcacaacttc
     2221 atagtgtctg gaagtattat ttttatgaca aaggaaatac tttgcgcaaa tattttttgt
     2281 ttgattacca gcgaaacgtt tctcttattt aaataattac ccacacaaaa tataggcttt
     2341 taattatcaa cctttattat aaataaaagc tatgaaatat attctcttcg ttaacttata
     2401 attaagagaa aaaaactcac cgcattgcca tttaagccag gaataaatta gcttcccgtt
     2461 catcattttt gtgatcttgc gcacatcttt gagataacgc actttcttca ccagtacgtt
     2521 gcacgctttg tcagcagaag attgcaacaa tctttcagaa ggccgcgaat cccccgtttt
     2581 gtcgtggtaa tgtatgcact ttgtgttaga cgggataggt ttttaagatg gaaaaactgc
     2641 gggtaggaat cgtttttggt ggtaaatcag cggaacatga agtgtctctg caatcggcaa
     2701 aaaacattgt cgatgccatt gataaaagtc gcttcgacgt tgtgctgctg ggcattgata
     2761 aacaagggca atggcacgtc cgcgatgcca gcaattatct gctaaatgca gacgatcctg
     2821 cccatattgc gttgcgccct tcggcgacca gccttgcgca ggtgccaggt aaacatgagc
     2881 atcagcttat cgacgcgcaa aacggtcagc cgttgccgac ggtggatgtc attttcccga
     2941 ttgtccacgg tacgctgggc gaagatggtt ccttgcaggg aatgctgcgg gtcgccaatt
     3001 taccgtttgt aggttctgat gttctggctt cagcagcctg tatggataaa gatgtcacca
     3061 aacgtctgct acgcgatgcc gggctgaaca ttgcgccatt tattaccctg acgcgcgcta
     3121 atcgtcacaa catcagtttt gccgaagtgg agtctaaact ggggttacca ctgtttgtaa
     3181 aaccggctaa tcagggctct tctgttggtg tcagcaaagt aaccagtgaa gaacagtacg
     3241 caattgccgt cgatctggcg ttcgagttcg atcataaagt gatcgttgag caagggatca
     3301 aaggtcgtga gatcgaatgc gcagttctgg gcaacgacaa tccgcaagcc agcacctgtg
     3361 gcgagatcgt actcaccagc gatttctatg cctacgacac caagtacatt gacgaagatg
     3421 gcgcgaaagt ggtagttccg gcagccattg caccagagat caacgataag atccgggcga
     3481 ttgccgttca ggcttatcag acgttgggat gcgcaggcat ggcgcgtgta gacgtgtttt
     3541 taaccccaga gaacgaagtg gtgatcaacg agatcaacac actgcctggc ttcaccaaca
     3601 tcagtatgta tccgaagctg tggcaagcca gcggtctggg ttacaccgat ctgatcacac
     3661 gtttgattga gctggcgctg gagcgtcacg ctgcggataa cgcattgaaa accacaatgt
     3721 aatattttaa cgcccgataa tattcttatt gggcgttttc agtttcttca tcgcgacgac
     3781 gaataatcaa ccccgccagc cagaaactaa tcacccacgt caccagaccg acggcatagg
     3841 tctgccaacc ttttgcttca aatcccagta cccccaccac gccattaaga atgaatataa
     3901 ggccaatggc gaacgcatag tagtgccagt cacggcggat ttttacaggc agatttatgg
     3961 cagctccgtt aacgaaaaac aacatccttg catattctgt aacaaccgtc tgtggtgtag
     4021 acaacatttt cgtaattatt aataaattta acttaaggtt gccattttgt gactcacaac
     4081 gaagattcac gcttcgggaa tattcccaac atcaatttac catgattctg atattgacaa
     4141 tctaaatgac ctgtcagact tgccataagc cgctggtatc acaccagaag agaagcagat
     4201 gacttccgga ggtttttatg gctgatttca ccctgtcaaa atcgctgttt agcggaaaat
     4261 atcgcaatgc ctcttcaacg cctggcaaca ttgcctatgc gttgtttgtg ctgttttgct
     4321 tttgggctgg ggcgcaattg ctgaacctgt tagtgcatgc gcccggcgtc tatgagcgtt
     4381 taatgcaggt ccaggaaaca ggtcgcccac gggtggaaat tggtttaggt gtcggcacca
     4441 ttttcgggct gatcccgttt ttagcaggct gcctcatttt tgcagtggtg gcgctatggc
     4501 tgcactggcg acatcgccgc cagtaaaatt tacgttgcca gatgcggcct gaaacgcctt
     4561 atctggcctg acattcctgt ttgatcactg tttcatacaa cgcgcccgcc gttcgtctac
     4621 gcgcttcgca aaccaggccg tagtcaggtt gcgggtgatt ttcgggcttt ccagttgaat
     4681 gccaggcaac atttcgcggg gtagtgattt acccgttttc gcttcggcaa gttgataaac
     4741 tttcttgtac agcgccgtct cctcaaaaga gaagctgtca cctttctcta actgacggcg
     4801 aatttcgctg tcgctcatcc ccagttttcc cgccagtttg cgcgttgcca gttcggtttt
     4861 cccgggttct ttactgtcgt aacgaattaa atcaccgtcc agtgccagct tcacgccgct
     4921 ggccttactg actgcgttct gaaacgcggc attacggctg gcgtaccagc cagcgttaaa
     4981 atcagcaaag cggtatatcg gtgcgctata gctggcggga taattcagta aatggtaagt
     5041 accaaaccac aacccgccgc ggcgactgaa gacttcctga cgaactgtac cgtccatttt
     5101 ccacggatac cctttggtat gctgttcggc aaaagcaatg ctgacctgca tcggcccacc
     5161 ggtgcgcacc ggattaagcg aaccaaacag cgtctgcccc attggtacca tgctgatcag
     5221 atcgtcaaaa atcgcgctca attgcttttc tgtacgcacc gaatctaacc gttcgctata
     5281 acttttgccg tttggcgatt tgatttttag cgcggtatga accagaaaag caggaatgtg
     5341 catccgttca gcacgacggt caatctcttg ccaggcgatc ttgcttaagc ccggaacggc
     5401 cggatctgcc tgataatttg actcctgctg cgccaccgcc agcaccgagt agacgttttc
     5461 cagcgtcggt gccagcccct gactttcaaa ggtggtggcg agatctttcg cccaggcatc
     5521 gcggtctttc acgctcgcag gcatcttctg ccgcacgacg ctcgctacat ctatcgcttt
     5581 ttcgcccttt ttcaacggtt gcggagcctg actactacac cccgctaaaa ccagaaccgc
     5641 caacaatgac agcgaagata gatgattgac gcgcgacatt acttctcctt tttagctcaa
     5701 ggtatgggtt acttcctgaa ttttgtcacc atccagctca tgttcaaagc tgcgcagacg
     5761 tttgtagata gacatcaact caaccagtgt ggtccatgag ttaatcaggt actggaaagc
     5821 accgcgaacc tgaccaaaaa cgttggtaat ctgcgtcatc aggccgagcg taatcgtacc
     5881 ggcaacaatt gacggaaaca gcaagaacaa accgaaaacg ttatcgacct gcaaatagag
     5941 aatgcgggcg atgttgaaat acatatagtg aaaatagagg cgaaaatagt ttttccgtac
     6001 ggcgctaaac agctcgcgta ccgtaggcgg cgtcgcgcgc gtggcatcgt cttcaccata
     6061 aaccagctct ttacggtagg cggcctctac tcgctggttt ttaaactcca gccccggcag
     6121 tttgatccct accactgcca gcaatccggt ccccatcagc gaccagacga tagcggcaat
     6181 caccagacca tacggaatgt gcccgataat cggcagctcc ggcacatgcg cggagagcgt
     6241 taccagcacc ggcaggaagg cgatcaacgt catgatggcg ttgataaaac tgacgcccat
     6301 attctccagc gttgaagcaa aacgcatggt gtcttcctgc acacgctgtg cggcaccttc
     6361 gatatgacgc agttgttgcc agttcgccat gtaatattcg ttcatcgccg tacgccagcg
     6421 gaacacgtag tgactgacaa agaagttgtt cagtacactg atcaccaccg cgatcagcgc
     6481 aatccccaga aagacgccca cttcgcggta aaattgttcg atagtgactt tatgcggcga
     6541 acttagcgcg gtttgaatca gatcatagaa cggcgcatac caggcgttga cggcgacccc
     6601 gacttccacc aaaaaccaag tgacgaagat aatcagcgca gtaccgagta ttgaccagta
     6661 ttgccaacgg tgcgggctgt agataaacca gaaaaatgca aaaagtccta cgcaaacaat
     6721 gtagtaagcg taaaaaatca ggaaatccaa cgaccagaaa cgcgcggcgc taatcgggat
     6781 ctgcccggaa gcgccggtga tacgtgccac ccagtcaccc ccaccggctt gccagaagat
     6841 aacggcaatc aatgcccaaa taaaggccga gagaaaaaac gttcccggct ttgggaaaaa
     6901 agacttaaac atatgcactc ctgctaactt cttatcgttt taacaactgc tgtgtattaa
     6961 ccgcatgacc gctacaaagc gtcagccaaa tgaaaccgcc cattgtgacc catagcaagg
     7021 cgcttagttc gccagcctga ccataagaat tgtttcgacg gatttcacaa accaaagatt
     7081 gtttacataa gcggattaac acgcgcagga tcacaaatgc aggcattggt gagcaggaaa
     7141 aagatcgtgt ttacgcgccg ttacgttttt aacactatta ttgcactaat tcgccctttg
     7201 caatccatca atgagtagta taaatacgct cagttacctt cattcaatct atggacacca
     7261 ccgttgaaac gtagtctgct tttttctgcc gtgctgtgtg cggcgtcatt gacctccgtc
     7321 catgcggcgc agccgatcac tgaaccggag tttgcctctg atattgtcga tcgttatgcc
     7381 gatcatattt tttacggcag cggtgccacg gggatggcgc tggttgttat cgacggtaac
     7441 cagcgcgtct ttcgtagtta tggcgaaacg cgacctggta ataacgttcg cccgcagctg
     7501 gattcggtcg tgcgtattgc ttccctcacc aaactaatga ccagtgaaat gctggtgaaa
     7561 ttgctcgacc aggggaccgt aaagttaaac gatccgctaa gtaaatatgc tccgccaggc
     7621 gcacgcgtgc cgacctacaa tgggacgccc attaccctag tgaatctggc aacccatacc
     7681 agcgccctgc cccgtgaaca gcccggtggc gcggcacatc gtccggtgtt tgtctggcca
     7741 acgcgcgagc aacgctggaa atacctttct acggcgaagc tgaaagccgc gccaggcagt
     7801 caggcggcgt actctaacct tgcgttcgac ctgctggcag atgcactggc gaatgcgtcc
     7861 ggcaagcctt atacccagtt gtttgaagag cagattaccc atccattggg gatgaaagac
     7921 accacctaca ccccctcacc ggatcagtgc cgccgtttaa tggttgctga gcgcggtgcc
     7981 agtccgtgca ataacacgct ggcggcaatt ggcagcggcg gcgtctattc cacgcctggc
     8041 gatatgatgc gctggatgca gcagtatttg tcatctgatt tttatcagcg cagtaaccag
     8101 gcagatcgca tgcagacgtt gatttatcag cgcgcgcagt ttacgaaagt gattggcatg
     8161 gatgtccccg gcaaagcgga tgcgcttggt ttaggctggg tatatatggc cccgaaagag
     8221 ggtcgtccga ggattattca gaagacaggc ggtggtggcg gtttcattac ctatatggcg
     8281 atgatcccgc agaagaatat tggcgcgttt gtggtggtca ctcgttcgcc gttgacgcgc
     8341 tttaaaaata tgagcgatgg catcaatgat ctggtgaccg agctaagcgg gaataaaccg
     8401 ttggttatcc ccgcatcctg attaatactc tgaaggcaaa cggttgatag cgaccagttt
     8461 gcctttattc atttcgatat agccgccttt acgcaaagca gcgagaactt ctgcgacgac
     8521 agaacgcgat atgcgtgtac gttgatggat ataattcatc acaccaatac gtgatcgcaa
     8581 ttcttcattc cagtcaatca ttgataataa tgttgcgcgg gtttgtttgg ctggtagatg
     8641 atatcccgta caatttcctt ctgatattaa tttgtatggt atatcgtttt tca
//
LOCUS       DAJVPP010000142         8680 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVPP010000142 DAJVPP010000000
VERSION     DAJVPP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.142"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            167..1201
                     /gene="rhaT"
                     /locus_tag="OQM34_003613"
                     /pseudo
     CDS             167..1201
                     /gene="rhaT"
                     /locus_tag="OQM34_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(1198..2064)
                     /gene="rhaR"
                     /locus_tag="OQM34_003614"
     CDS             complement(1198..2064)
                     /gene="rhaR"
                     /locus_tag="OQM34_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCS1453894.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(2120..2956)
                     /gene="rhaS"
                     /locus_tag="OQM34_003615"
     CDS             complement(2120..2956)
                     /gene="rhaS"
                     /locus_tag="OQM34_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1453895.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            3244..4713
                     /gene="rhaB"
                     /locus_tag="OQM34_003616"
     CDS             3244..4713
                     /gene="rhaB"
                     /locus_tag="OQM34_003616"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1453896.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADVCGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            4710..5969
                     /gene="rhaA"
                     /locus_tag="OQM34_003617"
     CDS             4710..5969
                     /gene="rhaA"
                     /locus_tag="OQM34_003617"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS1453897.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            6211..7035
                     /gene="rhaD"
                     /locus_tag="OQM34_003618"
                     /pseudo
     CDS             6211..7035
                     /gene="rhaD"
                     /locus_tag="OQM34_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            7045..7359
                     /gene="rhaM"
                     /locus_tag="OQM34_003619"
     CDS             7045..7359
                     /gene="rhaM"
                     /locus_tag="OQM34_003619"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1453898.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            7660..8106
                     /locus_tag="OQM34_003620"
     CDS             7660..8106
                     /locus_tag="OQM34_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS1453899.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            8117..>8680
                     /locus_tag="OQM34_003621"
     CDS             8117..>8680
                     /locus_tag="OQM34_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS subunit IIBC"
                     /protein_id="HCS1453900.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDISKGAPSFTWVVESIGYLGFTFM"
BASE COUNT         2096 a         2260 c         2294 g         2030 t
ORIGIN      
        1 ggttttgcag gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc
       61 agtaaatcga caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc
      121 atctcgtcgg agatgtgaag cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat
      181 tacgatgggg atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc
      241 gttcaaaaaa gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc
      301 gtggattatt ctgccgtggg ccatcagcgc cctgttacta ccggatttct gggcgtatta
      361 cagctcgttt agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat
      421 cggtaatatc aactatggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat
      481 cgccattggc attacgttga ttgtcggtac gctgatgacg ccaattatca acggcaattt
      541 cgatgtgctg attaacaccg aaggcggacg catgacgtta ctcggcgttc tggtggcgct
      601 gattggcgta gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa
      661 agccgaagag ttcaatctga aaaaagggct ggtgctggcg gtgatgtgcg gcattttctc
      721 tgccgggatg tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact
      781 tggcgtcgat ccactgtatg ttgctctgcc aagctatgtt gtcatcatgg gcggcggcgc
      841 gatcattaac ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tgtcgctaaa
      901 agccgacttc tcgctggcaa aaccgctgat cattcacaat gtgttactct cggcactggg
      961 cgggttgatg tggtatctgc aattcttttt ctatgcctag ggccacgccc gcattccggc
     1021 gcagtatgac tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat
     1081 cgtcgggctg gtgctgaaag agtggaacaa tacaggacgc cgtccggtaa cggtgttgag
     1141 cctcggttgt gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggtgaatta
     1201 atctttctgc gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa
     1261 caccaccgaa aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa
     1321 caggcggcta tgctggagaa gatattgcgc atgacacact ctgacctgcc gcagatattg
     1381 attgatggtc attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga
     1441 tgcctcatca caaaatttat ccagcgcaaa gggacttttc aggctagccg ccagccgggt
     1501 aatcagctta tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg
     1561 atggcgattc agcaacatca ccaactgccc gaacagcaac tcagccattt cgttagcaaa
     1621 cggcacatgc tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat
     1681 ccccacgcta cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc
     1741 tccctgccag tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac
     1801 cagatcgtta acggaagcgt aggagtgttt atcgtcagca tgaatataaa agagatcgcc
     1861 acgggtaatg cgataaggac gatcgttgag tacatgcaga ccattaccgc gccagacaat
     1921 caccagctca caaaaatcat gtgtatgttc ggcaaagaca tcttgcggat aacggtcagc
     1981 cacagcgact gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg
     2041 cgccaccgtg gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa
     2101 tacgttgaga agattcgcgt tattgcagaa agccatcccg tccctggcga atatcacgcg
     2161 gtgaccagtt aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac
     2221 agcgataggc gatgtcagtg acgctggcct cgctgtggcg tagtagatgt cgggctttca
     2281 tcagtcgcag gcggttcagg tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc
     2341 gatgtagcgt acgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat
     2401 cggcaaaatg gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca
     2461 ggttctcctg caaactgctt ttacgcagca agagcagtaa ttgcataaac aagatctcgc
     2521 gactggcggt cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct
     2581 gtcgcacctg ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca
     2641 gctcttgtgg cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat
     2701 acagcacatt ggtcagacac agattatcgg tatgttcata cagatgccga tcatgatcgc
     2761 gtacgaaaca gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac
     2821 ccgtgccatg ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg
     2881 cctgcgggag ccggggttct atcgccacgg acgcgttaca agacggaaaa aaatccacac
     2941 tatgtaatac ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga
     3001 ggtcaggttc ttaccttaaa ttttcgacgg aaaactacgt aaaaaacgtc gatttttcaa
     3061 gatacagcgt gaattttcag gaaatgcggt gagcatcaca tcaccacaat tcagcaaatt
     3121 gtgaacatca tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac
     3181 gagaaggtcg cgaattcagg cgctttttag actggtcgta atgaaattca gcaggatcac
     3241 attatgacct ttcgcaattg tgtcgccgtc gatctcggcg catccagtgg gcgcgtgatg
     3301 ctggcgcgtt acgagcgtga atgccgcagc ctgacgctgc gcgaaatcca tcgttttaac
     3361 aatgggctgc atagccaaaa cggttatgtc acctgggatg tggatagcct ggaaagtgcc
     3421 attcgccttg gattaaacaa ggtgtgcgag gaagggattc gtatcgatag cattgggatt
     3481 gatacctggg gcgtggactt tgtgctgctc gaccaacagg gtcagcgtgt gggcctgccc
     3541 gttgcttatc gcgatagccg caccaatggc ctgatgccgc aggcacaaca gcaactcggc
     3601 aaacgcgata tttatcagcg tagcggcatc cagtttctac ccttcaatac gctttatcag
     3661 ttgcgtgcgc tgacggagca acaacctgaa cttattccac acattgctca cgctctgctg
     3721 atgccggatt acttcagtta tcgcctgacc ggcaagatga actgggaata taccaatgcc
     3781 accaccacac aactggtcaa tatcaatagc gacgactggg acgagtcgct actggcgtgg
     3841 agcggggcca acaaagcctg gtttggtcgc ccgacgcatc cgggtaatgt cattgggcac
     3901 tggatttgcc cgcagggtaa tgagattccg gtggtcgccg ttgccagcca tgataccgcc
     3961 agcgcggtta tcgcctcgcc gttaaacggt tcacgcgccg cttatctctc ttctggcacc
     4021 tggtcattga tgggcttcga aagccagacg ccatttacca atgacacggc gctggcagcc
     4081 aacatcacca atgaaggcgg ggcggaaggt cgctatcggg tgctgaaaaa tattatgggc
     4141 ttatggctgc ttcagcgagt gcttcaggaa cggcaaatca acgatctccc ggcgcttatc
     4201 gccgcgacac aggcacttcc ggcctgccgc ttcatcatca atcccaatga cgatcgcttt
     4261 attaatcctg acgagatgtg cagcgaaatt caggctgcgt gtcgggaaac ggcgcaaccg
     4321 atcccgggaa gtgatgctga actggcgcgc tgtattttcg acagtctggc gctgctgtat
     4381 gccgatgtgt tgcatgagct ggcgcagctg cgcggtgaag atttctcgca actgcatatt
     4441 gtcggcggcg gctgccagaa cacgctgctc aaccagctat gcgccgatgt ctgcggtatt
     4501 cgggtgatcg ccgggcctgt tgaagcctca acgctcggca atatcggcat cgagttaatg
     4561 acgctggatg aactcaacaa tgtggatgat ttccgtcagg tcgtcagcac caccgcgaat
     4621 ctgaccacct ttacccctaa tcctgacagt gaaattgccc actatgtggc gcagattcac
     4681 tctacacgac agacaaagga gctttgcgca tgaccactca actggaacag gcctgggagc
     4741 tggcgaaaca gcgtttcgcg gcggtgggga ttgatgtcga ggaggcgctg cgccaacttg
     4801 atcgtttacc cgtttcaatg cactgctggc agggcgatga tgtttccggt tttgaaaacc
     4861 cggaaggttc gctgaccggg gggattcagg ccacaggcaa ttatccgggc aaagcgcgta
     4921 atgccagtga gctacgtgcc gatctggaac aggctatgcg gctgattccg gggccgaaac
     4981 ggcttaattt acatgccatc tatctggaat cagatacgcc agtctcgcgc gaccagatca
     5041 aaccagagca cttcaaaaac tgggttgaat gggcgaaagc caatcagctc ggtctggatt
     5101 ttaacccctc ctgcttttcg catccgctaa gcgccgatgg ctttacgctc tcgcatcccg
     5161 acgacagcat tcgccagttc tggattgatc actgcaaagc cagccgtcgc gtttcggcct
     5221 attttggcga gcaactcggc acaccatcgg tgatgaacat ctggatcccg gatggtatga
     5281 aagatatcac cgttgaccgt ctcgccccgc gtcagcgtct gctggcagca ctggatgagg
     5341 tgatcagcga gaagctaaac cctgcgcacc atatcgacgc cgttgagagc aaattgtttg
     5401 gcattggcgc agagagctac acggttggct ccaatgagtt ttacatgggg tatgccacca
     5461 gccgccagac tgcgctgtgc ctggacgccg ggcacttcca cccgactgaa gtgatttccg
     5521 acaagatttc cgccgccatg ctgtatgtac cgcagttgct gctgcacgtc agccgtccgg
     5581 ttcgctggga cagcgatcac gtagtgctgc tggatgatga aacccaggca attgccagtg
     5641 agattgtgcg tcacgatctg tttgaccggg tgcatatcgg ccttgacttc ttcgatgcct
     5701 ctatcaaccg cattgccgcg tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc
     5761 gtgcgttgct ggaacctacc gctgagctgc gcaagctgga agcggcgggc gattacactg
     5821 cgcgtctggc actgctggaa gagcagaaat cgttgccgcg gcaggcggtc tgggaaatgt
     5881 attgccaacg tcacgatacg ccagcaggta gcgaatggct ggagagcgtg cgggcttatg
     5941 agaaagcgat tttgagccag cgtgggtaaa cactgccgga tgcggcacga gcgccttatc
     6001 cggcctacgg gtcgaatgtc catgcgggct gtcaccctca ccccggccct ctccctgaaa
     6061 gggcgaggga gaaaaacata gcctcgtgcg gcaacggagc tgaacaccgc tccggacagt
     6121 cccctcgccc ctacggggag agggttaggg tgaggggaac aagccgcacc atgtctaaac
     6181 aaggcaacct aatttacagg aacacagaac atgcaaaaca ttacccagtc ctggtttgtc
     6241 cagggaatga tcaaagccac caccgatgcc tagctgaaag gctgggacga gcgcaacggc
     6301 ggcaacctga cgctgcgcct ggatgacgcc gatatcgcgc tttatcacga caacttccac
     6361 ccacaaccgc gctatattcc gctcagccaa cccatgcctt tactggcaaa tacgccgttt
     6421 attgtcaccg gctccggcaa attcttccgt aacgtccagc tcgatcctgc agctaactta
     6481 ggcgtcgtaa aagtcgacag cgatggcgcg ggctaccata ttctctgggg gttaaccaac
     6541 gaagccgttc caacttctga acttccggct cactttctct cccactgcga gcgcattaaa
     6601 gccaccaacg gtaaagatcg ggtgatcatg cactgccacg ccaccaacct gatcgccctc
     6661 acctatgtac tggaaaacga caccgcggtc tttactcgcc aactgtggga aggcagcacc
     6721 gaatgtctgg tagtattccc ggatggcgtt ggcattctgc cgtggatggt gcccggcacg
     6781 gacgaaatcg gccaggcgac cgcacaagag atgcaaaaac attcgctggt gttgtggccc
     6841 ttccacggtg tgttcggttc cggaccgacg ctggatgaag cctttggttt aatcgacacc
     6901 gcagaaaaat cagcacaagt attagtgaag gtttattcga tgggcggcat gaaacagacc
     6961 atcagccgtg aagagttgat agcgctcggc aagcgcttcg gcgttacacc actagccagt
     7021 gcgctggcgc tgtaaggagc aaacatgatc cgcaaagcct ttgtcatgca ggtaaacccc
     7081 gacgcccacg aagagtatca gcgtcggcat aatcccatct ggccggaact ggaagcagtg
     7141 ttgaaatctc acggtgtgca taactacgcc atttacctcg acaaagcgcg taatctgctg
     7201 tttgccatgg tagagattga atctgaagaa cggtggaatg cggtagccag tactgatgtt
     7261 tgccaacgtt ggtggaaata tatgaccgat gttatgcccg ctaacccaga taacagcccg
     7321 gtgagtagcg agctgcaaga agtgttttac ctgccgtaat tgcccaatgt gccggagatg
     7381 cctccggcac aatattttaa atttcgattt gaaaaattaa acatattaca tctcccggta
     7441 agttatattt ccctgataca ttgtgagtaa atcacaaaaa taatgaataa cccattaatg
     7501 attcatgtgg tttatttaaa taaccattat gtgcattact ccgcaaatct gacctttcac
     7561 tctgtctcaa attgtcattg tttaccgaca tatcgcctct catcacttaa tcgctccacc
     7621 attacaattg attaaaatat attttatgga gtccgattaa tggcagctct tactgcaagc
     7681 tgtattgacc tgaatattca gggcaatggc gcttattccg ttctgaagca gttggcgaca
     7741 atagcgttac aaaacggttt tatcaccgac tcacaccagt tcctgcaaac cctgctgctg
     7801 cgcgaaaaaa tgcactctac tggatttggt tccggtgtcg cagtgccgca cggtaaaagc
     7861 gcctgcgtta aacaaccgtt cgtattattc gcccgcaaag cgcaggctat tgactggaaa
     7921 gccagcgatg gcgaagacgt caattgctgg atctgcctcg gcgtgccgca aagcggcgaa
     7981 gaggatcagg tcaaaatcat cggcacactg tgtcgcaaaa ttattcacca ggaatttatt
     8041 catcaactgc aacagggcga taccgaccag gtgctggcct tgttaaatca aaccctcagc
     8101 tcataaggaa gtggcgatgg agtcatcctt acgtattgtc gcgatcacca actgccccgc
     8161 cgggatcgct cacacctaca tggtggcgga agccctggaa cagaaagccc gttctctcgg
     8221 ccataccata aaagtggaaa ctcaagggtc cagtggcgtt gaaaaccgct tatccagcga
     8281 agagattgcc gctgccgatt acgtcattct tgctaccggg cgtggcctga gcggtgatga
     8341 tcgcgcgcgg tttgccggga agaaagttta tgagattgcc atctccctgg cgttgaaaaa
     8401 tatcgaccag attttcagcg aattaccgac aaactcgcag ctttttgccg cagatagcgg
     8461 cgtgaagctt ggcaaacagg aagtgcaaag cggcagcgtg atgagccacc tgatggcggg
     8521 cgtctctgcg gcgctgccgt ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat
     8581 gctggtgcag ttcggtttgc cgtataccga tatttcgaaa ggcgcgccgt cgtttacctg
     8641 ggtagttgaa tccatcggtt accttggctt cacctttatg
//
LOCUS       DAJVPP010000143         8666 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVPP010000143 DAJVPP010000000
VERSION     DAJVPP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.143"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..270)
                     /locus_tag="OQM34_003622"
     CDS             complement(<1..270)
                     /locus_tag="OQM34_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCS1453901.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="OQM34_003623"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="OQM34_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="OQM34_003624"
     CDS             complement(1130..1336)
                     /locus_tag="OQM34_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCS1453902.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="OQM34_003625"
     CDS             complement(1443..2006)
                     /locus_tag="OQM34_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCS1453903.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="OQM34_003626"
     CDS             complement(3381..5216)
                     /locus_tag="OQM34_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453904.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="OQM34_003627"
     CDS             5317..5604
                     /locus_tag="OQM34_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1453905.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="OQM34_003628"
     CDS             5576..7105
                     /locus_tag="OQM34_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCS1453906.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="OQM34_003629"
     CDS             complement(7275..8492)
                     /locus_tag="OQM34_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1453907.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
BASE COUNT         2684 a         1965 c         1618 g         2399 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgcca
//
LOCUS       DAJVPP010000144         8518 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVPP010000144 DAJVPP010000000
VERSION     DAJVPP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.144"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..2694
                     /locus_tag="OQM34_003630"
                     /pseudo
     CDS             1..2694
                     /locus_tag="OQM34_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2804..3652)
                     /gene="alkA"
                     /locus_tag="OQM34_003631"
     CDS             complement(2804..3652)
                     /gene="alkA"
                     /locus_tag="OQM34_003631"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS1453908.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3786..5138
                     /gene="yegD"
                     /locus_tag="OQM34_003632"
                     /pseudo
     CDS             3786..5138
                     /gene="yegD"
                     /locus_tag="OQM34_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5150..7090)
                     /gene="yegI"
                     /locus_tag="OQM34_003633"
                     /pseudo
     CDS             complement(5150..7090)
                     /gene="yegI"
                     /locus_tag="OQM34_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7087..7848)
                     /locus_tag="OQM34_003634"
     CDS             complement(7087..7848)
                     /locus_tag="OQM34_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCS1453909.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVIFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(7845..8504)
                     /locus_tag="OQM34_003635"
     CDS             complement(7845..8504)
                     /locus_tag="OQM34_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1453910.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2078 a         2270 c         2219 g         1951 t
ORIGIN      
        1 ggctttcgat gctgtatctg ccgtggcctt ttactttcat tattgtgctg ttgatgtgga
       61 gcgccgtgcg cctgccacga atggaagcct ttttgatctt ccttaccacg gtgatgatgg
      121 tgtcgctgat gatggccgcg gatccctccc tgcttgctac gccgcgtacg tacctgatga
      181 gccatatgcc gtggctaccg tttttgctga tcctgctgcc cgccaacatc atgaccatgg
      241 tgatgtatgc ctttcgtgcg gaacgcaaac acatttccga aagcgaaacc cgctttcgga
      301 acgcgatgga atattccgct atcggtatgg cgttagtggg caccgaggga caatggctgc
      361 aatccaacaa agcactctgc cagtttctcg gttacagtca ggaagagctg cgcggactca
      421 cctttcagca actgacctgg ccggaggatc tcaataaaga tctccaacag gttgaaaagc
      481 tgataagcgg tgaaataaac acctattcaa tggaaaaacg ctactacaac cgcaatggcg
      541 atgttgtctg ggcgttgctt gccgtctcac tggtgcgcca cacggatggc acgccgctct
      601 attttatcgc tcagattgaa gacattaacg agctaaaacg caccaaacag gtgaatcagc
      661 aactgatgga gcgcatcacg ctggctaacg aagcgggcgg gattggcatc tgggagtggg
      721 agctgaagcc gaatattttt agctgggata agcggatgtt cgagctgtat gaaattcctc
      781 cgcatatcaa accgaactgg caggtgtggt acgagtgcgt gctgccggaa gatcgccagc
      841 atgccgaaaa agtgattcgt tgcaatcacg ctcgcccttt aagctggaat ttcgcattgc
      901 cgtgaaagat ggcatccgcc atatccgcgc cctcgccaac cgggtactga ataaagaagg
      961 cgaagtcgaa cgcctgctcg gcattaatat ggatatgacc gaggttaaac agcttaacga
     1021 ggcattgttt caggaaaaag agcgcctgca cattacgctg gattccatcg gtgaagccgt
     1081 agtctgtatt gatatggcga tgaaaattac ctttatgaat ccagcggcgg agaagatgag
     1141 cggctggacg caggaagaag cattaggtgt tccgctcctg acggtgttgc atattacttt
     1201 tggcgacaac ggaccattaa tggagaacat ttacagtgcc gacacctcac gttccgcgat
     1261 tgaacaagat gtggtgttgc actgccggaa cggcggcagt tacgacgtgc attacagtat
     1321 tacgccgtta agtactctgg acggcagcaa tataggctcg gtactggtga ttcaggacgt
     1381 caccgaatca cgcaaaatgc tgcgccagct gagctacagc gcctcccatg atgcactgac
     1441 gcatctcgcc aatcgcgcca gttttgagaa gcaactgcgt atcctgctgc aaacggtaaa
     1501 cagtacacat cagcgacatg ccctggtgtt tatcgatctt gatcgcttta aagcggtgaa
     1561 tgacagcgcc gggcatgcgg cgggtgacgc tttactgcgc gaactggcgt cgttgatgct
     1621 gagtatgctg cgctccagcg acgtgctggc gcgactcggt ggtgatgaat ttggtctact
     1681 gttgccagac tgtaatatcg aaagtgcgcg ttttatcgct acacgcatta tcagtgccgt
     1741 gaatgattat cactttatat gggaaggccg tgtgcatcgg gtaggtgcca gtgccgggat
     1801 taccttgatt gatgacaaca atcatcaggc ggcagaagtg atgtcgcagg ctgatatcgc
     1861 ctgttatgcc tccaaaaatg gtggacgggg ccgggtgacg gtttacgaac cacagcaagc
     1921 tgccgcacat agcgaacggg cggcgatgtc gcttgatgaa cagtggcgga tgattaaaga
     1981 gaatcagttg atgatgatcg cccacggtgt cgcttcgccg cggatcccgg aagcgcgtaa
     2041 tttgtggctg atttcactta agctctggag ttgcgaaggc gagattattg atgaacaaaa
     2101 atttcgtcgt agcttcagcg atccggcact tagccatgct cttgaccgac gggtattcca
     2161 cgaatttttc cagcaggccg caaaagcggt tgccagtaaa ggcataagca tcgccctccc
     2221 ctttccgttg ccggtttgag tagcgccacg ctggtgaatg atctgcttga gcagctggaa
     2281 aatagccctc taccaccacg gttattacat ctgattattc cggctgaagc gattttagat
     2341 cacgcagaaa gcgtgcaaaa actgcggctg gcgggatgtc ggatagtgct cagccaggtg
     2401 ggccgcgatc tgcaaatctt caactcgctg aaagcgaata tggcagatta cctgctactt
     2461 gatggtgagt tatgcgccaa cgtgcagggt aatttgatgg atgagatgct gattacgatt
     2521 attcaggggc acgctcagcg actcgggatg aaaaccatcg ccgggccagt cgttttaccc
     2581 ttagtgatgg atacgctttc tggcatcggc gtcgatctga tttatggtga tgtgattgcc
     2641 gatgcccaac cgctggattt gctggtgaat agcagttatt tcgcgattaa ctgagagagc
     2701 gtttgcccat tgcctgatgc gatgctaacg cgtcttatca ggcctacaaa tcgctcattc
     2761 cgcaggcagg ataaggcgct cgcaccgctt ccggcgacca acgtcatgct tcgtctggtt
     2821 gccagccttc cgtataccag atatgcaaca gcgcataaga acgccagggc ttccagcgct
     2881 cggcataacg gcggatttgt gccggtgtca tccccagaaa tcgctgttta atcagataat
     2941 catccggcag aaaaacatct ttcgcctgcc agccgcgcaa ggcgaaataa ttcgccgtcc
     3001 agcgcccgat acccggaaaa gtttgcagcg ttttcatcgc ctgctccaca tcgcccggta
     3061 ttgtcattgg taaggtgccc tccagcgccg catttgccag atgaatcagc gcctctgccc
     3121 gtttcaacgg catacctaac gcttttaatg cctgcgggtc ggctgctgcc agccgctgag
     3181 gcgtggggaa gcagatatac tccggaaaat catccagccg ttcgccataa agctgtgcca
     3241 ctctggcggt caattttgcc gccatcgcca cgctcaccag ttggcctaaa atcgcccgca
     3301 cgccctgctc aaaagcatca acactgccgg gtaaacgcaa tccgggccgc gccgcgccta
     3361 acttgcccaa cgcaccgtta acaatctgcg ggttacattg cagatcaagc aggcggctca
     3421 ttttcgccag acactcagcg gcaacgggtt ctaaacctgc acttaaattt atgtgcagag
     3481 tatggcgggc tatatccgga atagcagtca ccacgccgcg atattcgccc actgccagac
     3541 tacgggcata ataactgtcc gcgaccgttt ccacaccact caccgcacgg gcggcgagaa
     3601 atcccaacat ccacgaccag tcatacggcg gctgccagtt caggatatac atcgcatctc
     3661 cttattcatc cgctttcagc ataaacgtta ttcagacgtt acgctttgct ttcatattcc
     3721 ggttgtcgcg acggcaacat ttcgctaaag tcacgcccct tcttcaccgg catggggatt
     3781 atttcgtgtt tattggtttt gattacggta cagcaaactg ttcagtggcg gtcatgcgtg
     3841 acggtaaacc gcatttgcta aaaatggaaa acgacagcac gctgctgcct tcaatgcttt
     3901 gcgcgccaac gcgtgaagcg gtaagcgaat ggctgtaccg ccatcatgac gttccggcag
     3961 acgacgatga aacgcaggcg ttgttacgac gggcgattcg ttataaccgc gaagaagata
     4021 tcgatgttac agcgaaaagc gtgcagttcg gtctttcctc actggcacag tacattgatg
     4081 atccagaaga agtgtggttt gtgaaatcac caaaatcgtt cctcggtgcc agcggcttaa
     4141 aaccgcagca ggtagcgctg tttgaggatc tggtctgcgc aatgatgttg cacattcgcc
     4201 agcaggcgca ggcgcagctg ccagaagcga ttactcaggc ggtgattggt cgcccgatca
     4261 acttccaggg gctgggcggc gatgaagcaa acgcccaggc gcaggggatt ctggaacgcg
     4321 cggcgaagcg tgccgggttt aaggatgtgg tattccagta cgagccggta gcggctgggc
     4381 tggattacga agccaccttg caggaagaaa aacgggtgct ggtggtggat atcggcggtg
     4441 gtacgactga ctgttcattg ctgctgatgg ggccgcagtg gcgttcgcgt ctcgatcgtg
     4501 aagccagtct gctgggtcac agtggttatc gtattggcgg taacgatctg gatatcgccc
     4561 tagcgtttaa aaacctgatg ccactgctgg gtatgggtgg cgaaaccgaa aaaggcatcg
     4621 ccctgccgat cctgccgtgg tagaatgcgg ttgccatcaa cgacgttcct gcacagagtg
     4681 atttctacag tagtgccaac ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac
     4741 cggaaaaagt ggccctgtta cagaaagtct ggcgtcagcg tttaagctat cgcctggtgc
     4801 gtagcgcaga agagtgcaaa attgctcttt caagcgtagc ggaaacccgc gcctcactgc
     4861 cgtttatcag caatgaactg gctacgctga ttagccagcg agggctggaa agcgccctca
     4921 gccagccact ggcgcggatt ctggaacagg tgcaactggc cctggataac gcccaggaaa
     4981 aaccggacgt tatctacctg accggcggta gcgcccgttc tccgctgatt aaaaaagcgc
     5041 tggcagaaca gttgccgggc attccgattg caggcggcga tgactttggc tccgtcaccg
     5101 ccgggctggc acgctgggcg gaagtggtat ttcgttaaaa gaaaatggct cagcagcggc
     5161 ttaaatcagc ctgcgcctga gccagttttt ctgccgcctg acgtaacggt tccatcaacg
     5221 gcatggtccg tgctggagca tgaaggcgga atcgctgcaa ctctgtcgcg ccgacagtca
     5281 acgtcgattc cagccgatgt cgtttggcgg ccattttcgc cattaccgcc tgtctgtctg
     5341 ctggcgttac cgcttcgttc ggcctgaaca caaaacggcg ttcacagctc gctttccagt
     5401 cgatgaccgc ctgggtcaga tgatcaccaa accctttaac ttgcttaacg ccacgacggg
     5461 taacacctgc tgctgtttca ataccaaaag accataacgc cgctttacgc gcagggccaa
     5521 cgccgggaat agaggcaaca tcaataaaaa atccctccag aaacttctac ttctgccgtt
     5581 cccttgcagt gtcatgaagt gctgccagag cgagtttctc ttcttccggt aacccgagaa
     5641 tttcgtcctt cattttttcg agcatcgtcc gtttggcgat aaaaccttcc aggccgccta
     5701 cctgctggat ctggcggact aaatggtcat agtccatttt cgcacgatta aatgcctcac
     5761 ggcgttgctg aacttctgct ttgtacgctt tgcttgtcag actgccaata atccagatag
     5821 ccgccagcac aggaaccaaa ataatataac gcggttctgc ctgaaggccg caaagcagca
     5881 gcgataatgc tgacagtgcg atctcaagca gaatgatgta ttcgcgccgt aacaggccta
     5941 aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa tggcagttgc aatgctggcg
     6001 gtgctactga cgccatcacc atcgcccaga cttttgccag cacaaaatcc ccgccggtgg
     6061 taatgacctc ttcgccgaga tcaataaaat agataacgcc ttgattatcc agcgtacacc
     6121 acgggcagtc ggtcaaatga gcgggataaa catgcattgc cgaaacggga catttcttta
     6181 actgttggcg cagagaatca agtgccgcta cccacgcttt agccgtcgga cgcccggttg
     6241 ccacgccact ttccgtgaat gcctgctgaa acatggcttc aacatcactc ggtaacatcg
     6301 atagcggaat cgatcgtggc ggcggtttta aaccacgtcg ctgattatct gacgcgtagg
     6361 cataacggaa atgggtaata tccgtctcca gcgcattacc cgcatcagag ataagcggca
     6421 caccagaata aggatgccgg ccaccaaaca agacgtgaaa aatcagcaac gcaaggccaa
     6481 aattaccgtg attttctgtg cgttcaaagc cgacaaatga cgacaaggtt tgtagctctg
     6541 gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag tgtgccattg gcgttaatct
     6601 gaaaggagtc gctatcaatc aacaccactt tgctgtcgcg acctaccata aaactgttct
     6661 ggtttacgtc acccacgaca tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat
     6721 tgcgcgcaac atagagtaga aaatcccacg cacaatgagg gtaacgttga cgacgatgtg
     6781 ccgggctata gatcatatga atcggttctt taccagaaac ttttggcatc ataaaaccga
     6841 taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca
     6901 attgcgcgtc agctgtcgca gccataaagg caagtttgtc ctgttttaag gcgggtggcg
     6961 gcgtgtgata aatcttggcg acgctatcga caaactcatt gatatcataa accgcgcctt
     7021 caccgccttt gcccagttca cggcctggcg taacgcactc accagtagca gtatataaag
     7081 tgggtttcat ggtaaccaca gcgccagtgc caacgtttta tcatcatccg tacgctcgtt
     7141 taccgccggg ctggacaaaa actgctttaa caattcaggt aataaatcga gttgttcctg
     7201 cgttgctgac gccagtccat tgaaaaacgg ggtaaagaaa ggcacatgcg gagaattatc
     7261 caacatattt aacgccagtc gctggatacc atccgtaaat gctgcaactt tatgcacacg
     7321 ctcagtgctg gtgaaagttt ccagtctgga aacggcatct tcatcggtaa tgaagtgcgt
     7381 catattggca tattcaccca ccatcggcgt aagcggcaat tgcagaccgt gaccaaaatc
     7441 caccaccacg ccgccatcgc caatctgcat aatcaaggtg ccattagccg atgaaatcaa
     7501 tcccagaaac gtgcaggcaa aatcgcgaac tgctaattct ttggcttccg cctcggcaaa
     7561 aagtcgctga cgaacggtca gcacaatatc cgtggcgagg atatcattaa gccccaattc
     7621 cccaccctgc actttttgcg acatataagc catcgcttca ttgacggcga gcatcgcgcc
     7681 ttcgcccccc tgcgaaacgc tacctgcgcc atcagcaagg aatatcacca gtaatggctg
     7741 ctggtcattt agccaggcaa cctgcatctg gcaggcatcc tggcaaggca agtccgcgct
     7801 gatgtgcgac gttccaacag cggatgcata aaccagacgc cagctcacac tgacgtccag
     7861 ccttttggcg cttccagcac aacttccgtt cccggcgtgg agcgggaaac cgaacgcagc
     7921 gaactggata accagctaaa cagttcacgg aattgtaatc cttgcagagg caaaggctga
     7981 cgaacgctga tttgtgccag agtcttcata tccgccccct gaacaccgat ggaaaagaaa
     8041 gcaaaccttt tatcttcttc cccccgaaac actttgttgg ctgcagcctg ccactcatcg
     8101 attggtgcac catcggtaat caggaaaatc cacgggcgat aatacgaaat accatttgca
     8161 cgatactcac gtttccgctc ctctaccata tccagggctt tggtaatggc cgcacccatt
     8221 ggtgtatcgc cctgggcaaa caggatgggc ggaaaaaaat tagcggcgct ggtaaagggt
     8281 tgttccacat gcaccgggcc gaacgtcaca atgccgagtt ccactctttt taaagccagc
     8341 gaatcggcaa gcagctcatc gcgaaaggta accaatccgg tgttaagttc attaataggt
     8401 ctgccactca tcgagccaga aacatccagc agcagaatgc aggggcaacg tggttccggg
     8461 ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc tcataagaaa tcctttct
//
LOCUS       DAJVPP010000145         8492 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVPP010000145 DAJVPP010000000
VERSION     DAJVPP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.145"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..93)
                     /locus_tag="OQM34_003636"
     CDS             complement(<1..93)
                     /locus_tag="OQM34_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1453911.1"
                     /translation="MSRKTRRYSKKFKAEAVRTVLENQLSISEDA"
     gene            138..317
                     /locus_tag="OQM34_003637"
                     /pseudo
     CDS             138..317
                     /locus_tag="OQM34_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(326..724)
                     /gene="vapC"
                     /locus_tag="OQM34_003638"
     CDS             complement(326..724)
                     /gene="vapC"
                     /locus_tag="OQM34_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCS1453912.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(724..951)
                     /gene="vapB"
                     /locus_tag="OQM34_003639"
     CDS             complement(724..951)
                     /gene="vapB"
                     /locus_tag="OQM34_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCS1453913.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            1033..1404
                     /locus_tag="OQM34_003640"
     CDS             1033..1404
                     /locus_tag="OQM34_003640"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCS1453914.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKCL
                     IEAHNQAVDFAVRQVEALAST"
     gene            1425..6286
                     /gene="traI"
                     /locus_tag="OQM34_003641"
                     /pseudo
     CDS             1425..6286
                     /gene="traI"
                     /locus_tag="OQM34_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6306..7052
                     /gene="traX"
                     /locus_tag="OQM34_003642"
     CDS             6306..7052
                     /gene="traX"
                     /locus_tag="OQM34_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCS1453915.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7107..7667
                     /locus_tag="OQM34_003643"
     CDS             7107..7667
                     /locus_tag="OQM34_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS1453916.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7798..8010
                     /locus_tag="OQM34_003644"
     CDS             7798..8010
                     /locus_tag="OQM34_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS1453917.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8254..>8492
                     /locus_tag="OQM34_003645"
     CDS             8254..>8492
                     /locus_tag="OQM34_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1453918.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1950 a         2118 c         2705 g         1719 t
ORIGIN      
        1 agcgtcttca ctgatcgaaa gttgattttc aagaaccgtt ctgacagctt cggctttgaa
       61 ctttttagag taacgtcggg tttttctgct cattattagc tccttctgat gccattctat
      121 ttcaggaagg agtgtccgtt aaactcaggc tacctcagat cagtgaatcc ggtgaagtgg
      181 tggatatggc cgcttatgag gcatggcagc aggaaaatca tccggacacc tggctgcaga
      241 tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga acgcggggag gatgttgagc
      301 cgggagatga tttctgatga acaggtcagc tccagtcttc agttctcagg ccgcccacac
      361 gttcaaattc ccgggtgtta ttagtcacaa taatcagccc ccgactgcgg gcatgacctg
      421 cgatcatttg atcaaatggc ccgacagggc gtccctgacg ggcaagttct gctcttatct
      481 ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac atcaatgcgg gaaacaaatc
      541 cctcgatcac agcgagatta cgttcaggca tctggctttt ttctgcacca tatatcagct
      601 ccatcagggt gaccgaactg atacacattt tcccctggtt caggttaaaa cgctccctga
      661 cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt ggtatcgagc ataaacttca
      721 gcatcagaat gactcccttt cctgcatgcc gggctgttcc ctgttatcca taaaatcggt
      781 gctgacgctg tgtccgtcga accattcgtc ccatgtctct ccggctggcg taatgattct
      841 ggttcgtccg acagcaatca cttcaacgcg ttttacgttt tccggcaatg caaccgcttt
      901 tggcagtctg accgcctggc tgcggttgct gagaaatacg gtggtttcca ttatttgcct
      961 ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt gataccggga tatgtcaaag
     1021 ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg ccggaagtgc cggtaattat
     1081 tataccgaca aggataatta ctatgtgctg ggcagcatgg gagaacgctg ggccggccag
     1141 ggggctgaac aactggggct gcagggcagt gtcgataagg atgtctttac ccgccttctg
     1201 gagggcaggc tgccggacgg ggcggattta agccgcatgc aggatggcag taacaggcat
     1261 cgtcccggct acgacctgac cttctccgct cccaaaagta tctccatgat ggccatgctc
     1321 gggggggaca aatgtcttat tgaagcacat aaccaggccg tggattttgc tgtccgtcag
     1381 gtggaggcac tggcctccac ctgacggacg gacagtcaga aacggtgctg accggtaatc
     1441 tggtgatggc actgtttaac cacgacacca gtcgcgatca ggaaccacag ttacacacgc
     1501 atgcggtggt gactaatgtt acgcagtata acggcgaatg gaagacactg agcagtgaca
     1561 aagtggggaa aacggggttc attgagaatg tgtacgctaa tcagattgcc tttggcaggc
     1621 tctaccggga aaaactgaaa gagcaggttg aggcgctggg ctatgaaact gaagtggtcg
     1681 gtaagcacgg tatgtgggaa atgccgggtg taccggtgga ggccttttcc ggacgctcac
     1741 agactatccg ggaggccgtc ggggaagacg cctcgctgaa atcccgggat gtggcggcgc
     1801 tggatacgcg taaatccaaa cagcacgtcg atcctgaagt cagaatggcc gagtggatgc
     1861 agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg tgacgcagcg gaacaacggg
     1921 catatacccg cacgcagaca cccggacccg cttcacagga cgggccggat gtgcagcagg
     1981 cggtgacaca ggccattgcc ggattaagtg aacgcaaagt gcagtttatg tatacggacc
     2041 tgctggccag gacggtcggc atactgccgc cggaaaatgg tgtgattgaa cgggcacgcg
     2101 ccggtatcga tgaggccatc agccgtgagc agcttatccc cctcgatcgt gagaaggggc
     2161 tgttcacgtt cgggattcat atgctcgatg agctgtcagt ccgggcactc agtcgtgaca
     2221 tcatgaaaca gaaccgggtg accgtacatc cggagaaaag tgtcccccgg acggccggtt
     2281 acagcgatgc cgtcagtgtt ctggcacagg accgtccgtc gctggccatt gtgtccgggc
     2341 agggcggtgc agccgggcag cgtgagcggg tggctgaact ggtcatgatg gcccgggagc
     2401 aggggcggga ggtgcagatt atcgctgctg accgtcgctc gcagatgaac ctgaagcagg
     2461 atgaacggct gtccggtgaa ctgataaccg gacgtcgtca gttgctggaa ggaatggcct
     2521 tcacgccggg cagtaccgtt atcgtcgacc agggcgaaaa actctccctg aaagagacgt
     2581 taaccctgct ggatggtgcc gcacgtcata acgtacaggt cctgataacc gacagcgggc
     2641 agcgaaccgg tacaggcagt gcactgatgg ccatgaagga tgccggggtg aacacatatc
     2701 gctggcaggg gggagaacag cgaccggcca ccatcatcag tgaaccggac cgtaatgtcg
     2761 gctggccgga gattttgcgg ccagcgtgaa agccggagaa gagagcgtgg cacaggtcag
     2821 cggagtacgg gaacaggcca tactgacaca ggccattcgc agtgagctga aaacacaggg
     2881 cgtgctcgga cacccggagg taaccatgac cgccctgtca ccggtctggc tggacagccg
     2941 gagccgttat ctgcgggata tgtaccgccc gggaatggtg atggagcagt ggaacccgga
     3001 gacacgcagt catgaccgct atgtgacaga acgtgtgacg gcgcagagtc acagcctgac
     3061 cctgcggaat gcgcagggcg aaacgcaggt ggtgcgtatt tcttccctgg acagcagctg
     3121 gtcgctgttc cggccggaaa aaatgccggt ggcagacggt gagcgtctga gggtgacagg
     3181 gaaaattccc ggactccgcg tctccggcgg tgaccgcctg caggtggcat ccgtcagtga
     3241 agatgcgatg acggttgttg tgccgggacg ggctgaaccg gccaccctgc ctgtgagcga
     3301 ttcaccgttc acggcactga agctggagaa cggctgggtg gaaacgcccg ggcattccgt
     3361 cagcgacagt gcgacggttt ttgcctccgt cacacagatg gcaatggaca atgccaccct
     3421 gaatggtctg gcccgcagtg gtcgtgatgt ccggctgtat tcctcactgg atgaaacccg
     3481 tactgcggaa aaacttgccc gccatccctc ctttacggtg gtttctgagc agataaaggc
     3541 gcgtgccggt gagacattgc tggaaaccgc tatcagtctg cagaaagccg ggcttcacac
     3601 gccggcacag caggccattc atctggccct tcctgtgctg gaaagtaaaa acctggcctt
     3661 cagcatggtg gacctgctga cagaggcgaa gtcgtttgct gcagaaggaa ccggttttgc
     3721 tgacctggga ggggaaatca atgcgcagat aaaacggggt gatttactgt atgtggatgt
     3781 ggcaaaaggc tatggcacag gcctgctggt ttcccgtgcg tcgtatgagg cagaaaagag
     3841 cattcttcgc catattctcg aaggtaagga ggcggtcacg ccgctgatgg agagagtacc
     3901 tggcgaactc atggagaaac tgacatcagg acagcgcgcc gccacccgta tgatactgga
     3961 aacgtctgac cgtttcacgg tggtgcaggg ctatgccggt gtgggtaaga ccacacagtt
     4021 ccgggcggtg atgtcagccg tgaacatgct gccggagagt gaacgtcccc gtgtcgtggg
     4081 gctggggccc acacaccgtg cggtcgggga gatgcgcagc gccggcgtgg atgcacagac
     4141 actggcgtcc tttctgcatg acacgcagct gcagcagcgc agcggagaaa cgccggattt
     4201 cagcaacacg ctgttccttc tcgatgagag ctctatggtg ggcaataccg acatggcacg
     4261 ggcatacgcc ctgattgcgg ccggtggcgg tcgggctgtg gccagcggtg acacggacca
     4321 gctgcaggcc atcgcgcccg gtcagccttt ccgtctccag cagacgcgca gtgctgccga
     4381 tgtggtcatc atgaaggaga ttgtgcgtca gaagccggaa ctgcgggagg cggtatacag
     4441 cctgattaac cgggatgtgg aaagggcgct gtccgggctt gagcgtgtga aaccgtctca
     4501 ggtgccacgt ctggagggcg catgggcacc ggagcactcc gtgacggagt tcagtcacag
     4561 ccaggaagcg aaactggcag aagcgcagca gaaggcgatg ctgaaaggcg aggcttttcc
     4621 ggatgtcccc atgacactgt atgaagccat tgtccgcgac tataccggca ggacaccgga
     4681 agcacgggag cagacgctga ttgtcacgca cctgaatgag gaccggcgcg tactgaacag
     4741 catgattcat gatgcacggg aaaaggccgg agaactgggg aaggtgcagg tcatggtgcc
     4801 tgtcctgaac acagcgaata tacgcgacgg ggagctgcgt cgtctctcca cctgggagaa
     4861 taacccggat gcccttgccc tggtggataa tgtgtatcac cggattgccg gtatcagtaa
     4921 ggatgacggg ctgataaccc tgcaggatgc ggaaggtaac acgcggctga tttcgccccg
     4981 ggaggcggtg gctgaaggcg tcacgctgta caccccggac accatccggg tggggaccgg
     5041 tgacaggata cgcttcacga agagtgaccg ggagcgcggt tatgtggcca acagcgtctg
     5101 gacggtgaca gcggtttccg gtgacagtgt cacactttct gacggtcagc agacccgtgt
     5161 gattcgcccc ggtcaggagc gggcagagca acatattgac ctagcctatg ccatcaccgc
     5221 ccacggtgca cagggggcga gtgaaacctt tgccatcgcg cttgaaggca cggaaggtaa
     5281 ccggaaactg atggccggct ttgagtcagc ctacgtggcc ttgtcgcgta tgaagcagca
     5341 tgtgcaggtg tacaccgata accgtcaggg ctggacggat gccatcaaca atgccgtaca
     5401 gaaaggaact gcccacgatg tgtttgagcc gaaaccggac cgggaggtca tgaatgcaga
     5461 gcggctgttc agtacggcgc gggagctgcg ggacgtggcg gcaggccgtg ccgttctccg
     5521 tcaggcgggg ctggccgggg gagacagtcc tgcacggttt attgctccgg gacgtaaata
     5581 tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac ggcaagtccg ccggtatctg
     5641 gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg ggattcagcg gtgaaggtcg
     5701 ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag ggcagccgta acggagaaag
     5761 cctgctggct gataatatgc aggatggcgt ccggattgcc cgtgataatc ctgacagtgg
     5821 tgtggtggta agaatcgccg gtgaaggccg tccgtggaat cccggtgcca tcaccggtgg
     5881 ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg ggagccggaa atggtgaacc
     5941 ggtcacggca gaggtactgg cacagcggca ggctgaagag gccatccgcc gtgaaacgga
     6001 acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac aaacctgacc tgccggacgg
     6061 caaaacagag caggctgtca gggaaattgc cgggcaggag cgtgaccggg ctgccataac
     6121 tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa ccacaacggg tgcgggaagc
     6181 ggtccgggaa gttgcccggg aaaatttgct gcaggagcgt ctgcagcaga tggagcggga
     6241 tatggttcgt gacctgcaga aagagaaaac cccgggcgga gactgataca ggaagacaaa
     6301 cactgatgac aacggataac acgaacacga cacgtaacga ttcactggct gcacggaccg
     6361 atacctggtt gcagtcattt ctggtctggt cacccggaca gcgggatatc atcaaaacgg
     6421 tggcactggt gctgatggta ctggaccaca tcaatctgat attccagctg aagcaggaat
     6481 ggatgtttct ggccgggcga ggagcctttc cgctttttgc cctggtgtgg gggctgaatc
     6541 tgtcccgtca tgcgcatatc cggcaaccgg ccattaaccg gctgtgggga tggggaatta
     6601 ttgcccagtt cgcatattat ctggcaggct ttccttggta tgaggggaat atcctgtttg
     6661 cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac gcgcagtggg tggcgtacag
     6721 cagcggccat actcctgatg gccctgtggg ggcctttgtc cggcaccagt tacggtattg
     6781 ccgggttgct gatgctggca gtcagcaacc ggctgtaccg ggcggaagac agagcggaac
     6841 gtctggcgct ggtcgcctgc ctgctggctg ttattccggc gcttaacctt gccaccagtg
     6901 atgcagcggc ggtggccggt cttgtgatga cggtgctgac cgttggtctg gtgttgtgtg
     6961 ccggaaaatc attaccccgt ttctggcccg gagacttttt cccgacgttc tatgcctgtc
     7021 atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg gtatctttgg ccgcaagagg
     7081 atgattcgtc agaggcagaa cacagcatga cagagcagaa gcgaccggta ctgacactga
     7141 agcggaagac agagggaaca gcgcctgtcc gcagccggaa aaccatcatc aatgtcacca
     7201 cgccaccaaa gtggaaggtg aaaaagcaga agctggccga gaaagcggcc cgtgaagcag
     7261 agctggcggc aaaaaaagcg caggccagac aggcgctgtc catttatctg aacctgccca
     7321 cgctggatga ggctgtgaat accctgaagc cctggtggcc gggattattt gacggtgaca
     7381 cgccccggct tctggcctgc ggtatccggg acgtgttact ggaagacgtg gcgcagcgga
     7441 atatcccgct ctcgcataaa aaactgcgca gggcgctgaa ggccatcacc cgttcagaaa
     7501 gctatctgtg tgccatgaaa gccggtgcct gccggtatga cacggaaggg tatgtgacgg
     7561 agcatatttc tcaggaggag gaagcgtatg cgggagcgcg tctggcgaaa attcgtcgcc
     7621 agaaccggat aaaggcagaa cttcaggccg tgcttgatga gaaataaaaa aagcctcccc
     7681 cagagagagg aggcagggaa ataaggctta aaaggaaata acttccgctt ccgaaaggaa
     7741 gccagtccac tgaattattt atcacaataa tactgacaac taaaggggga caagaaaatg
     7801 aatggattca gaaacagttc acggaacggc caggtctggc gttaccagcg tgccggcggg
     7861 cgggccgtta ttctggaagt cagtggacgc tggatggaag cggcagaagc atggcgacgg
     7921 gctgcctgtg ttgcgcctag gacagactgg cagcagtttg cccggaaaag ggctgaacac
     7981 tgccaccggc gctgccgggg aagggtgtaa cgaaaaaagc agccttcatt actctaggag
     8041 tcagaaggct gaaaggagtt tatgagattt gcttcttaat atccggtcgg gcagtatgac
     8101 cggacagttc tgagtagcac agagtctttt ccgcattaag tcgatttttt atattttgtg
     8161 attagtttca caaaataagg tgttattcaa tgtgtgctgc actattcagg ggggctgaaa
     8221 tacaggtggc atttatctga tatggagaat aacatgagga aatatattcc actggtatta
     8281 tttatctttt catggccggt attgtctgca gatattcatg gacgggttgt tcgtgtactg
     8341 gacggtgata ccatcgaggt catggactca ctgaaggcgg tgcggatcag gctggtaaat
     8401 attgatgcac cggaaaagaa acaggattat ggccggtggt caacagacat gatgaaatca
     8461 ctggtcgcag gaaaaacggt taccgtcaca ta
//
LOCUS       DAJVPP010000146         8410 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVPP010000146 DAJVPP010000000
VERSION     DAJVPP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.146"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(322..396)
                     /locus_tag="OQM34_003646"
     tRNA            complement(322..396)
                     /locus_tag="OQM34_003646"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Gly,seq:tcc)
     gene            complement(400..475)
                     /locus_tag="OQM34_003647"
     tRNA            complement(400..475)
                     /locus_tag="OQM34_003647"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Thr,seq:tgt)
     gene            complement(478..554)
                     /locus_tag="OQM34_003648"
     tRNA            complement(478..554)
                     /locus_tag="OQM34_003648"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(518..520),aa:Arg,seq:tct)
     gene            complement(556..632)
                     /locus_tag="OQM34_003649"
     tRNA            complement(556..632)
                     /locus_tag="OQM34_003649"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(596..598),aa:Ile,seq:cat)
     gene            complement(832..1212)
                     /locus_tag="OQM34_003650"
     CDS             complement(832..1212)
                     /locus_tag="OQM34_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1453919.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDNAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(1205..1405)
                     /locus_tag="OQM34_003651"
     CDS             complement(1205..1405)
                     /locus_tag="OQM34_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCS1453920.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(1500..1757)
                     /locus_tag="OQM34_003652"
     CDS             complement(1500..1757)
                     /locus_tag="OQM34_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453921.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(1754..3145)
                     /locus_tag="OQM34_003653"
     CDS             complement(1754..3145)
                     /locus_tag="OQM34_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1453922.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(3142..4020)
                     /locus_tag="OQM34_003654"
     CDS             complement(3142..4020)
                     /locus_tag="OQM34_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1453923.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(4031..4939)
                     /locus_tag="OQM34_003655"
     CDS             complement(4031..4939)
                     /locus_tag="OQM34_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1453924.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(4926..5159)
                     /locus_tag="OQM34_003656"
     CDS             complement(4926..5159)
                     /locus_tag="OQM34_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453925.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(5156..6172)
                     /locus_tag="OQM34_003657"
                     /pseudo
     CDS             complement(5156..6172)
                     /locus_tag="OQM34_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            6104..6367
                     /locus_tag="OQM34_003658"
     CDS             6104..6367
                     /locus_tag="OQM34_003658"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1453926.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            6357..6665
                     /locus_tag="OQM34_003659"
     CDS             6357..6665
                     /locus_tag="OQM34_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1453927.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(6707..6883)
                     /locus_tag="OQM34_003660"
     CDS             complement(6707..6883)
                     /locus_tag="OQM34_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCS1453928.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(6880..7290)
                     /locus_tag="OQM34_003661"
     CDS             complement(6880..7290)
                     /locus_tag="OQM34_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCS1453929.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(7287..7565)
                     /locus_tag="OQM34_003662"
     CDS             complement(7287..7565)
                     /locus_tag="OQM34_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453930.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(7640..>8410)
                     /locus_tag="OQM34_003663"
     CDS             complement(7640..>8410)
                     /locus_tag="OQM34_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453931.1"
                     /translation="NTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCCKM
                     PPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCGIG
                     DDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITGLTDK
                     LFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLFLD
                     EQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         2026 a         2259 c         1931 g         2194 t
ORIGIN      
        1 cccgttgaaa ttttgtccat ggatttcata gcctcacctc cgcaaataac ggatggcgta
       61 gttttacact gagaaatgaa agggatttga aaagaaaaaa acgcaaaagc gggcgaaacg
      121 atatatacag taaggaaagc actctatcca acaaaccacc cacagttaat cggaataaaa
      181 gcagagtgct tatgaatgat cgcacgcccg aaggttagta tttctgcaca gcaattttgc
      241 aaaaaaaggc gatcattcat aacttaaacg tctttcagtc actccgggat ttcccatcat
      301 cgcagactga aagactctga ctggagcggg cagcgggaat cgaacccgca tcatcagctt
      361 ggaaggctga ggtaatagcc attatacgat gcccgcatat ggtgccgact accggaatcg
      421 aactggtgac ctactgatta caagtcagtt gctctgcctg ctgagctaag tcggcgctgg
      481 cccaccaccg aggactcgaa cctcgcaccg tcaacttaga aggttgatgc tctatccgga
      541 tgagctagtg gtggttggtg gcccttgctg gacttgaacc agcgacctgg cgattatgag
      601 tcgctcgctc tgaccaactg agctaaaggg ccggaggcag aataataacc atatgtcatc
      661 acatctgcaa actcatctga ccaccagcgt gtttaacgtc ctgtaccgtt tttcaggcat
      721 aaaaaacccg cccggaggcg ggtttaagct gtgtggcgaa gcaaccactc ttaacagaat
      781 atcccgtttt ttacgtacgt aaaatatttt ctagaaagtc gccccttacc atcagggatg
      841 ttcaatatat ttgtccattt ctaaccggac acccaacatc atcaacattc cttccaccac
      901 cccctcggct ttttgtagct tctttcctat ataaccatct gagcatccat gcttccgcgc
      961 cagcgtcatg aatgtcatcc caaacacgta gtagtcaacc agcaagtcat gtagatcgtt
     1021 gttgttccgg ttaaggcggg ccatacaccc acatataacc atcgcgttat cgtcacagca
     1081 ttgcgggcgg gattttactt ttgaagggat taatccctta aaaccggcag caatggacga
     1141 ccaggtcaca tcctcatgat tattagccac ccacgcgccc cagcgctcaa gaacctgctg
     1201 aatatcacgc atcactgtct ttacccctgt cccatccacg atgaacaatc agaacaccat
     1261 caacaatggc gtgccctttg ccctctttat cttcagcata ttttcttacc gtggcgcgat
     1321 tacagttcag cattcgtgaa acttcggtca tattacctcg tgtctggata agcagttccg
     1381 gtatcgtttg aattttgacg ctcatcaaat actctccggt tcggtgattt ttatcccggg
     1441 ctttccacca gggacataat gatcgcgcac aagccagggc aaaacaaact catggcgcat
     1501 cagcgcagcg cctcctgcac cagtttttca aactttccga ctctggtttc cagctctgcc
     1561 acacagtcca ccagctcatc tactgctttc tgtgcgcgat gcttcgcctg catcagttcc
     1621 cgaagcgcgg gtaccatatc tttacggata gcatcttttg ttatgcccgt tttttcgagt
     1681 tgttcagcat gacgcagcat ttcctgcgcg tgtttacgca attgttcagg ggtaaaagtc
     1741 attgtctggt tgttcaaaag aaacgctcca tcttactgct gtcagttcgt ttattactgt
     1801 atctgcgcgg attgccgggc ttcatgggag tggaaagcac ccgtgcactt tcctggtcca
     1861 caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt tcccagagaa ccgttacgtt
     1921 gtttcgtgat attgatttct gcgatgcccc tggcctgcgt atccgggttg tacacttcat
     1981 ccctgtaaag catcagaatg atgtctgcat ccgcctctat ttctccggaa tttttcaggt
     2041 ctgagttcat gggacgttta ttgggtctgg actccacacc gcgggagagc tggctcagcg
     2101 caatcaacgg aaaaccaccg gattttgcca ggcctttaag cccctttgag atttcaccca
     2161 cggcaaggtc atgacgcccc gtggttcggg tttttatcag cccgagataa tcaaccacca
     2221 ccagcgccgt ttccggatat ttaatcaggt ggtgtttcgt tgttgcgcat atctcatcaa
     2281 tggccaggtt cgcctggtcc accatccaga tattgcgccc ggtcatccgc cccacccctt
     2341 gtgagaaacg cgtccagtct tcatcttcaa agtgagccac agatttcagg cgtgatactg
     2401 gcatccctcc agccgcagac accatacgtt caccaatctg gatgttcgcc atctccatgg
     2461 tgaacagaag cacaccatgc ccctgctcag tcaccttgtc gatgatgtcc agcgcaagtt
     2521 cggttttccc catcgaagga cgggcggcaa tgaataccag gtctccgggc tccataccgc
     2581 ctgtttttgc gtccagttca tcaataccgg tcatcaacgt cctggatttc tccagcccct
     2641 gattccggca ttcaacacgc tcaaccactt ccggaagcac atcatcaata tgtaccggct
     2701 gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt ctgcgcatcc ttcagggcat
     2761 cttcagctgc ttcacaggta tgcgcatcac gtaatttctg cagcgcctca ttcagtgttt
     2821 tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc aacatagtgc tccagtgaag
     2881 acttcaccca ggttttacgc ccggtatcag taatcaccgg ggcaagttcc ggcatctcat
     2941 tacacaacag cacggggtca atcactcctg aaacacgggc ctgtctgcag atgcctgtat
     3001 agatatcctg atacgctcgt acagaaaaaa cgtccgctgg cagtgtggcc agaatatcca
     3061 tcacttcagg atctgctccg cgcagaaaga acgcgccgat gacagcgcct tcaaggtcat
     3121 cgttacgcca tactggagtt gtcatgcagc cacacctctg atacgagaac ggtaactggg
     3181 ccagttaaac gacaaccagt tgcgtccccc gtctgtgatc ctgtcggcaa ttcggggact
     3241 gatgaacgcc cacaactctt ccggtgagag gttactgatc agaatggtgg gcaagatact
     3301 ttcgtacctg gcgttgataa tttcctgcaa aatagccatt tcagccgcgc tgccaaactg
     3361 aacgccaact tcgtcgatga tcagcaaatc cattgacgca taacgctcaa taacttcatc
     3421 cgctgttttt tcgctgtcat tccgccagca atttttcaca gcacgggtaa ggcgcatcac
     3481 gtcggtgatc tccacactgg ccagatagtt acggatgatg tgttttgcca tagccacagc
     3541 cagatgattt tttccggtac cacaactgcc ggtcataaca agactggtac cgttctccag
     3601 catatctggc cagttctccg catagcggcg acaggccgca agatttctgg ctgcgccagg
     3661 attaacctcc agataattat caaactcgca gtcccgaaaa cgcagggcaa ttccggcgtt
     3721 atcagtcagc tcttccgcct tgatggacga cagctccatg gtcaaatcgt tggcctcagc
     3781 gattaagcag tcagggcagc atgaaatttc ttctctgtcc tcgccattac gatcgctcca
     3841 caccagtata tgcgtgtgat attcgccatg tttttcgcaa tatccgcgac cttcacgcat
     3901 caggcaggaa cgataaggcc atggcttttc gcccttctga gcaaatgcaa tctctgcccg
     3961 taactcatcc attcgcgcct gtagtctttt ttgttgttca cgcaggttaa acgtcatcat
     4021 cgctgtcacc tcagaatgtc agtttgtcac tggatttacc gaatttgtca gacatggctc
     4081 ccaggccagc caggacatcg acctgtcgct gtcgcccacc tctgggagcg gctggctgtt
     4141 gccagaagtc ttcgaagtga cgatcgggtc caaagaacgt cgcagcctgc ttcacgaact
     4201 gtgtgccggt atttcctgtg gcacgtaccc aggcagcata gcgtttcacg ccatcaagca
     4261 tggcttcagg ttttattccc tccctgagcc gggctttcca ggctttgaag gctgcggatt
     4321 ttgagttacc accagcacgt tttggatatt cctgccaggc ctgttcaaat tccggtgaat
     4381 attcctgtcg ggaagaacgc gctggtgcag acgcgtcagc ggatgcgcca atagtgtttt
     4441 tactctctgt agtattctct gaagtaatct ctgttgtatt ctctgtaaga tcgaaattgg
     4501 ttttcccttc tccgcggcga ggggtttccc gtgtccgcgg tgaaggcttt ccctcctccg
     4561 cgaaattggg ttttacagtt tcccgaaaac gggtttcccc atttcgggaa aactgattgt
     4621 tttcattgat aatttcatta aggcgctcac aatctatacg gtagaacatt ttgtgctcaa
     4681 gacgcttgtt ggtttcaacc aaaatgcctc tggacacaag atgcttacgc gctacagcct
     4741 gttgttcaaa tgtaagtccg gtttcgtgtt gtatctcttc acgcgtttta tgtacgcctt
     4801 ccgctgcatg tgctttatcc tgccagtaaa aaatctgacc aaagaaaata acagcgtgcg
     4861 gacttcccat gtatttaacg agcccagggt aataagcaac cggatgtcca aaatcgagca
     4921 gaagatcaga cggacgcata gccacctccc aggcgtttaa acatttttcc gggctgaaac
     4981 gccaccagcg gataactcag ggtatgagta cgtccctgaa cctggcagac aaccttctgg
     5041 ctttcggtac tgaccaggca aacccgcaga acgtagccgt tgctggtggt gaaccactgc
     5101 cccacacggg ggcaatggtt gtatcggtga tacaggaaat taacgatgtg gcggatcatg
     5161 ggcgcacctc cttgtcagaa ccattcagcc tggaatcaac aagtgcagca ccaaaaacag
     5221 catcacctac acggtcgtac agtttgccag ccagcggaga ttcaacggcc ttaagcattg
     5281 ggtaaagctg gcttgtccag atttgatgga tttcacgcaa atgcaggtat acgcctctgg
     5341 cgttttgtgc gacagatgac atgtcagccg caccagctcc tgataagctc ctctccatct
     5401 ggttaaaggc attgatgtat gcctctttga accgggcggc acgtttacca gtgaagccca
     5461 tagcaaggaa ggcgaagccg ccgcgggtaa tttggtagca gggaagtttg cgagtaccgc
     5521 cgttgggctg gcgtaccaaa attgatgtct ccgcaaaatt gcgggcacaa aactctggag
     5581 aacaatccaa aatgcggatc tttttcagaa catcgtcatg acgtttagag aagaagttgg
     5641 caacagccag ggatgaagta acagcctaac catcaacgat ggcaatttca ggttgagaga
     5701 gggttgggag agtagtcatg gtgacagccc ctatgttgaa ttcaaagaac tcaccacatg
     5761 ggacgccaat cacagaggtg gtgagacgta cagggttggc gtaaccggtc aacatagaac
     5821 ccggcgcatc ttgcgatgtc cctgcacgcc ccaccataat ttgggcgtag caatgctcat
     5881 gacacgaaaa aaccgcatga gcgcggttat gctctatatt gaatttcagg acgccaatcc
     5941 cggcccccgc tttataaggt gcagagacag tgtaacgtcc cgaaattgca gaatcaatat
     6001 attgtgcaga tataacgatt ttgttatgtt ttgctgtatg aactacacta tcgaatacta
     6061 cagtgaagag gtccgactgg aggtcgatca gcttccattg agtatgcgtg ccagatacca
     6121 gcatcttgtt gaacgcatga aggtatacgg cagcaatctc ggagaacctc acaccagcgc
     6181 ctttggtgac gggcttttcg aactcagaat taaaggcaac gatgggatcg cgcgtgtttt
     6241 ttactgcact ctgacaggga aacgcatcat catgctgcat agttttgtaa agaaagcgca
     6301 gaaaacaccg ccagccgaac gcaagaaagc tgaaaccaga atgaaggagg ttaagcatga
     6361 ctggtaaacg cactctcccc accatgacac acgacgaaat ggcagccaaa tggatggaag
     6421 acccggcttt taaagcagaa tacgacgcta tcgctgacga attcgcactg cttgatgaaa
     6481 tgctggcagc acgcaaagaa gctggcttaa ctcaggctga agttgccgag cgaatgggaa
     6541 caaaagcgac cgcgatcacc agaatggaaa gtaatctcgc atcaggtatc agcggcccat
     6601 catttgccac actaaaaaaa ttcgcccgcg ctactggaaa aaaactccag atccgcttcg
     6661 tttaaccaca ccgcgccgtc attctggcgg cgcaccggat aaatagtcat ttcctcgcac
     6721 gatgtcttag ccaccggata tcccacaggt gagccgtgta gttgaaggtt tttacgtcag
     6781 attcttttgg gattggcttg cgtttatttc tggagcgttt cgttggaagg tatttgcagt
     6841 tttcgcagat gatgtcggtg aaacttcgtc gatgtcgcct cattcatacc tcctgtcggt
     6901 aaatctgaca ccctgaccaa tagcccaggc tgttgtgtac tcgatcagac ttgccatgcg
     6961 cttcacgctc atctgcgcgc tgctttcgcg aatgttgacg cattctcctt caaggccggg
     7021 caaaacatca gcttcctgtt ttgttgccac tgcatgaccg ctgatcaaca aaaccttcca
     7081 ttgttctggt tttaaccatt tgccgcacca ttgaacctga cgagcgatat ccgccagcat
     7141 cgcgtgaaat tttgcgttct ggtcaagatt tcgcttgtag tcagtaatgc ggatggtgac
     7201 tggcttgtct ttatcgagag gagttgagag gatggcattt attgcggctt gctgttgttg
     7261 cttagttcgg aggaagattg tttgcttcat cgaaaattct tctctttaat tccagcggct
     7321 ctgatagctt tcattactgc aattaccgtt ttgtccttcc catcctcatg ccccatcgca
     7381 taagcacctt cttcaccatc tttccaaaag tcgtcattcg attcgggcca gtcgatatcc
     7441 agttcaatag ctgctcgcga tgcctgccat atcacccaag caaactcttt taattcatcg
     7501 tctcctgtga actggctttt gtcttttgac caccagtttt caaactgtcg gtagctatcg
     7561 ttcacttccc tctcccccaa ataaaaaggc ctgcgattac cagcaggcct gctattagct
     7621 cagtgatgta gatggtcatt taatactccg tcacgttttc ctgtcgccac gcctcgtcat
     7681 attccgattt cggcatattg gcgatgtagc tatatggcga tcctgattca agttgcagga
     7741 actggtgcga ttgctcgtca aggaacaacg gaacaccacc ttcccaacct tcgccgttac
     7801 gttgtttttc aagcatcaaa acagatgccg gagatgccag tagctgttcg tccttctctg
     7861 acatcttttc accactctga actctctgta acgctctctc gcgagccttg ttacgccaga
     7921 tgatgaaaag tttgtctgtc aggcctgtta tcgctccaga gccttttacg tccattttcc
     7981 cggttggttt ttcttcgctg tctccttttc gcgagtgagt aacgagaatg acgtgggagt
     8041 ttgttttgtt tttgaagtcg caaatcgagt caacaaacgc cttctgcccg ttatagtcat
     8101 cgtcgcctat gccacatttc atcaggctgt cgatgatgaa taactggatc ccgtatcggc
     8161 ggcgagcgta gtcgaatatt tcgatcagcc tgtcggcttt cgccgttcca gtcaggccaa
     8221 acacccaaag tctttcgtca taaaatttaa atgcagagtc aatttccagc actggcggca
     8281 tcttgcagca cgttgcctga cgggtaagtc gcttaaggag aataccaggc ttcagctcaa
     8341 gtgacgcgat gcacgtcttc acaccctgac gcattgcctc aagtgccata tgcccgacaa
     8401 cctcggtgtt
//
LOCUS       DAJVPP010000147         8381 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVPP010000147 DAJVPP010000000
VERSION     DAJVPP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.147"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            423..1118
                     /gene="minC"
                     /locus_tag="OQM34_003664"
     CDS             423..1118
                     /gene="minC"
                     /locus_tag="OQM34_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1453932.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1142..1954
                     /gene="minD"
                     /locus_tag="OQM34_003665"
     CDS             1142..1954
                     /gene="minD"
                     /locus_tag="OQM34_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1453933.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1958..2224
                     /gene="minE"
                     /locus_tag="OQM34_003666"
     CDS             1958..2224
                     /gene="minE"
                     /locus_tag="OQM34_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1453934.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2596..2940)
                     /locus_tag="OQM34_003667"
                     /pseudo
     CDS             complement(2596..2940)
                     /locus_tag="OQM34_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2975..3094)
                     /locus_tag="OQM34_003668"
     CDS             complement(2975..3094)
                     /locus_tag="OQM34_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1453935.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3055..3240)
                     /locus_tag="OQM34_003669"
     CDS             complement(3055..3240)
                     /locus_tag="OQM34_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453936.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3341..3514
                     /locus_tag="OQM34_003670"
     CDS             3341..3514
                     /locus_tag="OQM34_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453937.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3516..3860
                     /locus_tag="OQM34_003671"
     CDS             3516..3860
                     /locus_tag="OQM34_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS1453938.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3864..4199
                     /locus_tag="OQM34_003672"
     CDS             3864..4199
                     /locus_tag="OQM34_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1453939.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4256..6577)
                     /locus_tag="OQM34_003673"
     CDS             complement(4256..6577)
                     /locus_tag="OQM34_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1453940.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6694..6903)
                     /locus_tag="OQM34_003674"
     CDS             complement(6694..6903)
                     /locus_tag="OQM34_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453941.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7654..>8381)
                     /locus_tag="OQM34_003675"
     CDS             complement(7654..>8381)
                     /locus_tag="OQM34_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1453942.1"
                     /translation="QPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIV
                     PLTQSLMNQVARQMNAIASKLPEGFHIGINFSASHIISPTFVDECLNFRDSFTRRDLN
                     LVLEVTEREPLNVDESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDH
                     SFVGRVNADPESTRILDCVLDLARKLSISIVAEGVETKEQLGYLNQNNITFQQGYYFY
                     KPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2349 a         1941 c         1828 g         2263 t
ORIGIN      
        1 agaagtatca gacgtcttct gcggtttcta tgatagagca attctgtggc ggatattaag
       61 ataacgggta tctgttcttt aatgatgctg caaaaaatgg aaagttaaga aagcctggcg
      121 tggtgaaaaa atacacttgc ctcaatactg gatatttaaa caggtctgga gatgctgtca
      181 cgattagggt gcggaaaaag gaagcgtaaa taagaagaat caaaagtacg acggcaatgg
      241 gttgattgac aagggtattt tttaagctat gaatcagcgc catttatcac agaatagact
      301 tttactctga ataaatggga gggtgacttg cctcaatata atccagacta taacatgcct
      361 tatagtcttc ggaacatcat cgcgcgctgg cgatgattaa tagctaattg agtaaggcca
      421 ggatgtcaaa cactccaatc gagcttaaag gcagtagctt cactttatct gtggttcatc
      481 tgcatgaggc agaacctaag gttatccatc aggcgctgaa agacaaaatc gctcaggccc
      541 ccacattttt aaaacatgcc cccgttgtac tcaacgtcag tgcactggaa gacccggtaa
      601 actggtcagc gatgcataag gcggtttcgg caaccggttt gcgggttatt ggcgtaagtg
      661 gctgcaaaga tgcgcaactt aaagccgaaa ttgaaaaaat ggggctgcct atcctgacgg
      721 aaggaaaaga aaaagcgcca cgtccagctc ccgcaccgca ggctccagcg caaaatacaa
      781 tgccggtcac aaaaacgcgt ttaatagata ccccggtgcg ttccggtcag cgtatttatg
      841 ctccacaatg tgatctgatt gttacaagcc acgttagcgc tggggccgaa ttgattgccg
      901 atgggaacat tcatgtctat ggcatgatgc gcggtcgtgc gctggcaggg gcaagtggtg
      961 accgggaaac gcaaatattt tgtacgaacc tgatggcgga attggtgtcc atcgcaggtg
     1021 aatactggct gagtgatcaa atcccagcag aattttatgg caaagcggcg cgactgcagt
     1081 tagtcgaaaa cgctttgacc gttcaaccgt taaattgatc cctttttaac aaggaatttc
     1141 tatggcacgc attattgttg ttacttcggg taaagggggt gttggtaaga caacctccag
     1201 cgcggccatc gccactggtt tggcccagaa gggaaagaaa actgtcgtga tagattttga
     1261 tatcggcctg cgtaatctcg acctgattat gggttgcgaa cgccgggtcg tttacgattt
     1321 cgtcaacgtc attcagggcg atgcaacgct aaatcaggcg ttaattaaag ataagcgtac
     1381 tgaaaatctc tatattcttc cggcatcgca aacacgcgat aaagatgccc tcacccgtga
     1441 aggggtcgcc aaagttcttg atgatctgaa agcgatggat tttgaattta tcgtttgtga
     1501 ctccccggca gggattgaaa ccggtgcgtt aatggcactc tattttgcag acgaagccat
     1561 tattaccacc aacccggaag tctcctcagt acgcgactct gaccgtattt taggcattct
     1621 ggcgtcaaaa tcacgccgcg cagaaaatgg cgaagagcct attaaagagc acctgctgtt
     1681 aacgcgctat aacccaggcc gcgtaagcag aggtgacatg ctgagcatgg aagatgtgct
     1741 ggagatcctg cgcatcaaac tcgtcggcgt gatcccagag gatcaatcag tattgcgcgc
     1801 ctctaaccag ggtgaaccag tcattctcga cattaacgcc gatgcgggta aagcctacgc
     1861 agataccgta gaacgtctgt tgggagaaga acgtcctttc cgcttcattg aagaagagaa
     1921 gaaaggcttc ctcaaacgct tgttcggagg ataagttatg gcattactcg atttctttct
     1981 ctcgcggaag aagaacacag ccaacattgc aaaagaacgg ctgcagatta ttgttgctga
     2041 acgccgtcgc agcgatgcag aaccgcatta tctgccgcag ttgcgtaaag atattcttga
     2101 ggtcatttgt aaatacgtac aaattgatcc tgagatggta accgtacagc ttgagcaaaa
     2161 agatggcgat atttctattc ttgagctgaa cgtgacctta ccggaagcag aagagctgaa
     2221 ataagcccgc tgtaaaaacg catttatctt caaggcagag tcatttctgc cttgagtttt
     2281 tcatccctct catccacgtt gtggtaaagc ggcgagtatt cttgctgata ctcctcattg
     2341 ctatttccac ctcccccttt acaccttaag gctgttccca cgcacagaac aaaatgttta
     2401 tgcttggttg agtaaataac catgttatta gtgtgtatat actgggagat gataaccccg
     2461 caatttcatt attcattgtt ctcccatagg atgaggtttc ccgccgtaac tggcgggctt
     2521 tttttgcccc agaattttct ctttcaacat cctgtaaacg aaaactgcgc cgaagcgcag
     2581 tttaatcgtt gatggtcaga acgaccagtt cacaccagcc accgcgttcc acggggactc
     2641 cacaccggca ccatggctat accccacccc gaaatgcccg cttaacgtac tgctgaatga
     2701 ggctttaata cctgcctggt atattccacg tctgcccgac aaatcattga caaaattacc
     2761 gtcactattc actttcaccc gattatcatc gacaaattct ttgcgcacag ccgccttcag
     2821 gcagggttca atttccatac cgttccccag acgcatgttg taactcagcg ttgcacccag
     2881 ttcacgatat atactgcggg tatcgactga tttcgattcc atgccattgg ataaatgata
     2941 gtagcgtcta agtgacctaa gctctgctgc aacatcagcc cgcaggcacc agaccagggg
     3001 attcatcctg aagagacagc gcaggtgtat tgtgttcacc gctcaccaaa gacatcatga
     3061 tgaaatgatg atatcccgca taagaaagag gcatttttta cacgcagtgc gctgaagtgt
     3121 agttggataa aaaatcactc ttttcagata atactgtcgt acgcttatct ttagacaatg
     3181 aggccgtcgg aaaaataaaa tgactaatgt aatcgttgct gatgaatatc ttctgctcac
     3241 ataaaaatca cacaataata ttgataaatc agattatctt acctttacaa cattgcgtta
     3301 acagtcaatg acacttcttg aataaaaata ttgtcaatca atgagttatt cctcatttaa
     3361 gattattctc attcacgtaa aaaacataat tcctatcatc acagccacgc tcattttgaa
     3421 ttatctgaat aatagcgagc gttctttagt taagcagatc ttaattgaag acgaaattat
     3481 tgtgtgtgca acttacttaa taccggacat ctaagttgaa gaaaaaaata cttgccttcg
     3541 ggcttatctc agcattgttc tgctccacgc cagcgatggc ggatatgaac cgcaccacga
     3601 aaggtgcatt gctaggcgca ggcgttggtt tacttactgg caatggtgtt aatggcgtac
     3661 tgaaaggtgc agctgttggc gctggtgttg gtgcagtaac agaaaaaggc cgcgacggta
     3721 aaaatgcgcg taaaggtgca aaagtgggtg ccgccgttgg tgctgtcact ggcgttctga
     3781 ccggcaatgg cctcgaaggg gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa
     3841 ttctgggtaa aatgaaataa ggaatgatga tgaaaaaatt tgctttactg gcgggcctgt
     3901 ttgttttcgc accaatgacc tgggcgcagg actacaatat taaaaacggt ttgccgtcag
     3961 aaacctatat tacttgcgca gaagccaacg aaatggcaaa aactgacagc gcacaggttg
     4021 cagaaattgt tgcggtaatg ggtaatgcca gcgttgccag ccgtgattta aaaattgagc
     4081 aatctccgga actgagcgcc aaagtggtag aaaaactgaa tcaggtttgc gctaaagacc
     4141 cgcaaatgct gttgatcacg gcaattgatg acaccatgcg tgctatcggc aaaaaataat
     4201 atttcatatg taccgcgtcg atgaatacga cgcggttttt tagctaacgg cgggatcaga
     4261 acttatattt caggcccacc atcactgcgg tgtcattgta gccattatca cccagctgca
     4321 cgcccacatt cccccagacg ctggccgctg gattcaggtt gccatttacg ccaagtttaa
     4381 tttcccccaa attactcacc ccatcttgtt cgactttcac accgttcatt gagacagcaa
     4441 aatctttact gttatggatc cagttcattt caacaaaagg ttcaaagtcg tcaccgtgcg
     4501 gaccgctgtt tttctcctga gtacgaataa aggtgcggaa gccgagtcgg gtctggatgt
     4561 tgttatttgc gtcattctca atacgtgagc cattggcttc cgtgtgcgca ttatgatcga
     4621 cgcccatcca gatagcctgt gcctgtggct ggaagatcca cgtatatttc gccgcttcca
     4681 gaccaaagga tttattcagg gcaaaaccgt aacccgcttc cagcgagacg gttgcacctt
     4741 tcgcgctata ggattcatag gctaattcat cccctttcac ggagttttta aaccagctat
     4801 attgcgccca ggagtcaatg tatgcgcctt ttttactgat gtcatcggca aaccaagtgg
     4861 catacagccc tgcgctatag cctctgacac tccctttcga acgataatcc gacacgctgg
     4921 aatgagttaa gttgtagtcg cgggcataac cagccatcac tcccaaacgc caactgtcac
     4981 tatcggtaaa accaccggtt aacagatcgc cccctaactg cgagacgtag cgatgcgagg
     5041 tcgttctcaa ctgtccgttg ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc
     5101 aaagtcggct tgaacgctcc tgttcagtta caggatcgat gtagcgcgtt tcacccgcac
     5161 ggtcgttcag acgcataaca aaaagagtgt tagcggctgc caggttggcg acgtaacttc
     5221 cagcttcggg gcgcaggacg ggggtaggcg caacagtagg cgctgaactt cctccggagt
     5281 ttgtttcacc gccgctcgtt gcctggaagt tagtcagata ccagttttta ttggtcgctt
     5341 cggtattacc tcgggttaat gaatattcgt aggcaccggc gaaaatcctc gaatccttaa
     5401 taaattcccc atttgattcc ccctcaacgc tgataatctc tatcccattt aacgtgtaag
     5461 ctccgctgcc tcctgcattg gtaactttaa ctcgggttgt tccagaggta tttccattaa
     5521 taacgacttt atccgtagct gaattatcat catccaattc agttctgaga tgcaatacgc
     5581 cattattacc aacgtaattt tcagttattg ttaatcttgt tggttcgaca tcccgtccat
     5641 ctgcccggga aatatagacc gtactattat caaccgtgag gttgctcacc gttgagtcag
     5701 ctttaatatc ccaggtacta ttatctgaaa gcgacagata ggtatgagtg ttatcatctg
     5761 ttgaaatatt agctgaaccc gttatttttg aattagttgc atcgacggtc aaatgacctt
     5821 taatattatt gataaaaaca tctgcagccg atgatacctc actatccgta atggtcacgt
     5881 tactcgtctc atttttatgg tagaggattt catttgctga ccgtagtgag ctaccagata
     5941 aaatatctac cgttgcttcc tgaccattta tggaagactc cagcacaatt ccagacccct
     6001 gatgcgcgac tgcagatgta tttttcagcg tcaccgtgcc gccattataa attttagcaa
     6061 tcgcggcgtc aggacccgtt gtataaattt ctccctggtt aaatgaaact gtcccctggc
     6121 gggcagcgat ggcatgagca ttgtccccat gcgtggtgat atcaacatcg gttaatgtaa
     6181 cgctggcatt ggtataatca agcgtgtcac cagccttgac gacagtgcca ttagcgtacg
     6241 cggcataact ttctgttccg tgggttgtga tctccccttt tgccatatcc agttttgcta
     6301 tggcattatt ttcattactt cctgtatcgg cagtactttc ttcatcaaga gaagatacac
     6361 acgcacacgc gcttccggga gatgcacacg caagaacgcc gactgcgtga ttacccgcag
     6421 taacaataaa tgtatcatct ccgtcagaaa aagagggggt aaccgaaccg tcagagttga
     6481 ctcttattat ggtgtcagta ttcaggtttc cactcgtttg acttagcaaa ccaattgagt
     6541 aatcccccgt tgtgacaatc gtgtttttac cctgcaaaat aacagcaccg ccaaattcac
     6601 ttacgattgc gctagctgtt aatgctgggc ttagaggact attgtcagaa ggatataaac
     6661 cgcccgttgt tacaatgacg ccattgttaa tggttaactt actaccctct cctgcaacgg
     6721 atacccgtaa cttccattac ccgttgttat aactagcgca ttatcaatat cttttacttt
     6781 tccatctttt attgccacag catgtttata ttcatcgtgc tgaataaact gttcagcata
     6841 tgcattaccc accgccagag caataagtcc caaactaaac ccggggagtt ttttcagttt
     6901 catagattaa ctatccattc attggattaa caaacattcc tggtcaaata acctatgagc
     6961 tcagattacc aaaacggagg gtaaagacaa aaacaaaagc acaaaaaatt taaatagata
     7021 aacagcgaca atatataaat gataaataag tcggcattat catttttcac cttattttca
     7081 tgacattgat cactttgggg tgactatttt gtttaacaaa taactcttcg acatcaaaat
     7141 tattaatagt tgccatggta attatttcgg tgatatataa aaacaactgt gataacacca
     7201 aggttgacaa ctatttgcgc aataattcct ttccttcaat gatccaggcg ttcactgcca
     7261 gcaacccatt cagccgcata ctgacagtga gaaactgaaa gattattgct gatccctgtg
     7321 gctaacgagt ctttctctta aatcgattaa ccagattgat cgccagccaa acaagggcat
     7381 taagcaaatt tatcgtcaca agcacttctc ccagcccggt actgggtttt gcaataagga
     7441 tgaccaggag ggtgatcagc ataaatgcaa tggaaaagat gatatagaga gtgaaataat
     7501 ccagattatt actattattc atgctactaa tggcaatcct gtcaaaactt tcaaacaatt
     7561 atagcaaaca aaataatgaa caatagtaac aagaggcaac gagctaatta gctgtctata
     7621 attacaccgc actatggcct tactgatcaa ttttcactca accataacct tcaccttcgg
     7681 tttagaaagg ataattttga ccaggtcgat gtatgtaaca ggtttataga aataataacc
     7741 ctgctgaaat gtgatattat tttggttcag atagccaagt tgttctttcg tttcgacacc
     7801 ttcagcgacg atactgatcg aaagtttacg cgccagatcc aatacacaat ccagaattcg
     7861 ggttgattct gggtctgcgt ttacgcggcc aacgaaactg tgatcaattt tgatataatc
     7921 aatatgcagg tcatgaagat aagaaagccc tgagtagcca gtaccgaaat catccagcgc
     7981 gatgacaaaa ccattttcat gcagaatgtt caaccgctga accagacttt catcaacatt
     8041 caatggctca cgctcggtga cttccagaac aaggtttaaa tcgcggcggg taaaactgtc
     8101 acggaaattt aaacactcgt cgacaaacgt cggcgaaata atatgcgaag cgctaaaatt
     8161 aattccaata tgaaaacctt ccggcagttt actcgcgata gcgttcatct gtctggcaac
     8221 ctgattcatc aggctttgtg taagcggaac gattaatccg gatttttcag caagtggaat
     8281 aaatgccgcg ggtgaaatat atccaccgtg aggttgtttc cagcgggcta acacctcaac
     8341 tccccgcaat gtcccttccc gaccatttac cacaggttgg t
//
LOCUS       DAJVPP010000148         8229 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVPP010000148 DAJVPP010000000
VERSION     DAJVPP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.148"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(60..2222)
                     /gene="uvrD"
                     /locus_tag="OQM34_003676"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(60..2222)
                     /gene="uvrD"
                     /locus_tag="OQM34_003676"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1453943.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2306..3022)
                     /gene="yigB"
                     /locus_tag="OQM34_003677"
     CDS             complement(2306..3022)
                     /gene="yigB"
                     /locus_tag="OQM34_003677"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1453944.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3022..3918)
                     /gene="xerC"
                     /locus_tag="OQM34_003678"
     CDS             complement(3022..3918)
                     /gene="xerC"
                     /locus_tag="OQM34_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1453945.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(3915..4622)
                     /locus_tag="OQM34_003679"
     CDS             complement(3915..4622)
                     /locus_tag="OQM34_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1453946.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(4619..5443)
                     /gene="dapF"
                     /locus_tag="OQM34_003680"
     CDS             complement(4619..5443)
                     /gene="dapF"
                     /locus_tag="OQM34_003680"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1453947.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5480..5683)
                     /locus_tag="OQM34_003681"
     CDS             complement(5480..5683)
                     /locus_tag="OQM34_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1453948.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            5872..7350
                     /locus_tag="OQM34_003682"
                     /pseudo
     CDS             5872..7350
                     /locus_tag="OQM34_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7347..>8229
                     /locus_tag="OQM34_003683"
     CDS             7347..>8229
                     /locus_tag="OQM34_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCS1453949.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGI"
BASE COUNT         1899 a         2283 c         2113 g         1934 t
ORIGIN      
        1 tattgaatct gaatgatttt ttaggccaaa taaggtgcgc agcaccgcat ccggcaacgt
       61 tacaccgact ccagccgggc gtaagctgcc accagccatt tgattccctg gccctggaat
      121 gccacctgca aacggctatg ttcaccgctg ccttccatat tgacgatggt gccttcacca
      181 aacttagcgt ggcgtacacg ctggccgagc ttgtagccgc tgtcgttctc gaccatcggc
      241 gtacccatcc gctgatggct gaccgggcgg cttaccgtgg cgcgcaggcg cacctcttcc
      301 acacactctt ccggcaactc gccaataaag cgcgacgggc gatggtaaac ctctttgcca
      361 tacagacggc gagtttccgc gtaggtcagc gtcagtttct gcatcgcgcg ggttacgcca
      421 acgtaggcca gacggcgttc ttcttccaga cgcccgcctt catccagcga catctggctt
      481 gggaacatgc cctcttccat accgacgata aacacctgcg ggaactccag acctttcgcc
      541 gagtgcatgg tcatcagttg taccgcatcc tgccaggtat ccgcctgccc ttcgcctgct
      601 tccagcgccg catgggatag gaacgcctgc agcggcatta aatcttcgtc ttcttcgttg
      661 tagctgaact ggcgcgttgc cgtcaccagt tcctctaagt tttcgatacg cgtctgacct
      721 ttttcgccct tctcctgctc gtacatggta cgcaggccgg agtctttaat tacccggtca
      781 gtctgtacat gcagcggcat atcggcagtt tcctgcgcta aggcgtcgat cagttccata
      841 aaccgctgta aggcgctggc agcacgtccg gcgagggctt tttcctgcaa cagttcacga
      901 catgcctgcc agagtgttaa ctggcgatcg cgcgatgtct gacgtaccac gtccagcgtc
      961 cggtcaccaa taccccgcgt tggcgtattc accacacgct caaaggccgc gtcgtcgtta
     1021 cggttggcaa tcaggcgcag atacgagagc gcatctttga tttcctggcg ttcgaagaag
     1081 cgcatcccgc cgtaaatacg gtacggcata ctcgcctgca acagtgcttc ttccagcaca
     1141 cgcgactggg cgttactgcg gtagagaatg gcgcactcgg caagcgcccc gccgttgtcc
     1201 tgccaggttt tgatgcggtt aaccacaaaa cgcgcttcat cgagttcgtt aaaagcgcaa
     1261 tagagggaaa taggctcacc gtccgcgcca tcggtccaca gttttttacc cagacgcccg
     1321 ttattgtttt caatcagggc gttagcggcg ctcagaatat tgctggtaga gcggtagttt
     1381 tgctccagac gaataatttc ggcaccgggg aaatcattaa ggaaacgctg aatattctcc
     1441 acctgcgccc cgcgccagcc gtagattgac tggtcgtcat caccgacgat catcactttg
     1501 ccggtgtcgc ccgccagcag gcggatccac gcgtactgaa tgttgttggt atcctggaat
     1561 tcgtccacca ggatattggt aaaacgttcg cggtagtgtt gtaggatatg cggcttgtta
     1621 agccacagtt cgtgagcgcg cagcagcagc tcggcgaagt ccaccaggcc cgcgcgatcg
     1681 cacgcttcct gatacgcctg atacaccttc tgccaggtct gctccaccgg attaccgtag
     1741 ctttgaatat gatgcggacg caggccttca tctttctggc tgttgatgta ccacattgcc
     1801 tggcgcggcg gccactgctt ctcgtcgagg ttcatggctt tgatcagacg cttaagcagg
     1861 cgtagctggt cttcgctatc gaggatctgg aaatcctgcg gcagattggc gtccatatgg
     1921 tgcgcacgca gcaggcggtg cgccagcccg tggaaggtgc ctacccacat gccgccctgg
     1981 ctggtgccca tcagttgtcc gatacgatga cgcatctccg ccgccgcttt gttggtaaac
     2041 gtcacagcca taatcgagta tggcgagcag ttttccacgc tcatcagcca ggcgatacga
     2101 tgcaccagta cgcgcgtctt accactgccc gcgcccgcca gcaccagaag gttgctgcgt
     2161 ggcgcggcca ccgcttcgcg ttgtttgtca ttaaggctgt cgagcaggta agaaacgtcc
     2221 attggcaccg ccgcgtaaaa ataggttggg cggagaagcg caacgccctc cgccaaaaag
     2281 ctgggtatat atacagattt gctgattata tcagcgaggt cagagatgcc aaccgggaaa
     2341 tttccagatg tggcagtaaa cggctgtccc aggtttgcat cagatcgcca ttttccggtc
     2401 tgatccaaca agcctgcatt ccgctgcgaa ttgccccacc cacgtcagtg gtgagatcgt
     2461 ccccaacatg taagatctcg ccgatcggca cgttgagttt ttccgcagcc aaaaagtaca
     2521 tatcgctgaa cggttttgag cgcccgtgcg ggccagcgcg cagcacaaac tcaaaataat
     2581 cccccaaacc aaacagttcc ggctgggcgt taccgttggt gatcgccacc agcggccatt
     2641 tcttcgccag ctgttttaag gtgtcgtgag tttgctgcgg gacgtcgatt cggctgcgcc
     2701 atttggcaaa gttaatcatt gctgcgtgtg cgcctgcact ggcttcttcg gcactcagcc
     2761 cggcgtcgag catcgcttgt tcaatcgaac gaaaacgcca acgtgtcacg tcgtgataaa
     2821 tctcgggttc cgcttcccgt accgcctggc gcaggcgttg cagatcttca ttctggaagc
     2881 tgcgcagcgc cggatgataa ttttgcacaa aggtaagcgc ctctcgctcg gtgcgcaaaa
     2941 tcaccggacg gttatcgtaa agggtatcat ccaggtcaaa ggtgagcgcc gagatgcgcc
     3001 ccaaaggccg gtaaaaacgc attatttccc ccgtttggcg cgtggatgcg ccgcatcgta
     3061 caccgaggca aggtgttgaa aatcaagatg agtatagatt tgcgtggtgg agaggttggc
     3121 atgacccagt agctcctgca caccacgaag atcgccgctc gactccagca tatgcgtagc
     3181 gaacgagtga cgtaatttat gtgggtgaac gtgattattc agcccttgtt ttatgcccca
     3241 ttcggcaaag cgtttctgca cattacgcgc ggagatacgc ttgcccagtt tcgacagaaa
     3301 aagcgcgtcg tcttcgctac caaacaggtc gcgcaaatca agccagtgct caatccacgc
     3361 cacagcgttg cgaccaatcg gcaggcggcg ctctttgctg cctttcccca tcacccacac
     3421 ttcgccggac tccaggtcga ggtgtttgat atccagcccc accagctcgg aaagacgcag
     3481 acccgcgccg tacatcactt ccagcattgc acggtcgcgt acagcgaggg gatcgttgat
     3541 atcaatatcc agcagccgat tcatatcgtc gacgtcgata tttttcggca gatgacgcgg
     3601 cgctttcggt gccgaaactc ctttcgccgg gttagctttg agttcgttct ggctgaccag
     3661 ccagtcaaaa aagctacgta gcgcagaaag ccgtaacgcc agacttgctg ctcccagccc
     3721 tttacggcga ctgcgtacag caaaattgcg taccatcgct gcatcgcatt gttgccagct
     3781 ttgcaggccg ttttcgctgg caaaattgat gatcgcctca agctgacgct ggtagttaag
     3841 tagggttatc gggctaagct gacgctccac gctcagataa cgtaggtagc gttctacatc
     3901 ggtgtgtaaa tcggtcatac gcgttcaatc caacgctcca gaagctccgg caacatcagc
     3961 gcaatttcat gaagtaactg cgttccctgc ccttgttgat agtgactggc atcgcgactg
     4021 gtaaacagca caacacccaa atcagcatcg cttcccagca tcgacatcgc caccgatccc
     4081 accgctttcg cttccggtag caccaccagc agctccggtc cgttaagcgg cccaagatag
     4141 tgctgttcct gccccaaacg ctgaatacgc agcggttcga aagactgacg gcttaatgcc
     4201 agatgagtgt ggttcgacgg cgcacctaag cgccagcgat ctggaaacag gcgcagactc
     4261 gcaccagcta ggccgagatc gcgcgcccag cggtgaaagc gcatcagcat atcgtcgaga
     4321 ctgctggcgg cggtgagact gcgctgcagg tagagtaggc gataaaacag cccttcgttg
     4381 gcgatagcct gctccatcaa caacgccatg ttctcttcca gaacatgaat gtgattacgt
     4441 gcgcgggcca tgtgccactc gaccaacgaa acggtgccgc gtaccggatg cggcacacgt
     4501 atcgcttcta ctgcgcgcgc attgcggata aaaaactcag gatttttaat cagataatcg
     4561 acaaccgccc ggtcatcaag ctccgtgagt gtttcctgca gttcttcccc tggttgcttc
     4621 atagatgaat aaatccatcg tagacatgta ccgccgggcc agtcatatat aacgggtgac
     4681 ccggaccttt ccaggcgata tcaagacgac cgccggggag ttccacgcgt acttcttcgt
     4741 ccagcaaacc ttgctgaatc cctaccgcca ccgccgcaca cgcgccgctg ccgcaggcct
     4801 gggtttctcc tgccccacgc tcataaacgc gtaaacgaat atgctcgcgc ttaaccactt
     4861 gcataaaacc gatattggcg cgctccggaa aacgctcgtg actttccaga acaggaccaa
     4921 gcgtttctac cgccgcggta tcgacatcat cgacctgaat cacgcaatgc ggatttccca
     4981 tcgacaccac gccgcataag attgtctgct cggcggcgcg cataatatag gtcttttccg
     5041 ctttgttagc gcgaaacggc acggcggaag gttcgaagtt gggttcgccc atatttacgc
     5101 ggaccagatc atcatcggtg acggtcagaa ccatccgccc gttggcggtg ctgacgcgga
     5161 tatcacgctt attggtcagt cctttcagac gcacaaaacg ggcaaagcag cgcgcaccgt
     5221 taccgcactg cgccacttca ctgccatcag cattgaaaat gcgatagtga aaatccagtt
     5281 caggatcata cggcggctca accaccagca gctggtcaaa ccctaccccc aggtgccgat
     5341 ccgccaggcg acgaatcagc tccggtgaaa aaaagacatt ctgcgttacc gcgtcgacga
     5401 ccataaaatc gttgccaagg ccatgcattt tcgagaactg catcatttac tccaatcacg
     5461 cgggtacaga aactgacttt tagtaattca cctgggatgg accatcgcca gtggcgcgat
     5521 cgtttttatc cggcaccgtg gattgcgttt gcgtctctac cggtttggtc ggcggcggtg
     5581 cgtttttatc tgcaggcggg aaatagagcg gacctttcag accgcagccc gtcaggctga
     5641 agagagtaag taatacagtg agtgccttaa acacgttttt cattatggat tgcctgtaaa
     5701 gttcatcgct ttctgctttc tatcatcgca ggagaaggcg taaaagcaag cgtttagcga
     5761 ctctttatga agtctgaaaa gtgaggaaat gctgcgagta taacgcttac tcgtgaggat
     5821 ttatgttctg ctggcagttt cgtgatcaac acctcgcgcc aggatggctt tatgcactat
     5881 cgcaatgtgg tttgtttgtt gtcttgttca ttgtttttat cgtcggcatg gggatgtcgg
     5941 ctttccgcac ctgaacataa tatttaccag aaacagggaa aaggcgtggt gtacctgcgc
     6001 ccttataaag aaacaaatct ttctctacct gacgtgaatt acaagagttt gcgtcggtta
     6061 cccaatttat taatagaccc gacaacacta gacgaatggg ataaagaacc gcctctgact
     6121 gatttgacaa ccgactacct ctatgagggc gcacaggcct ggtatcctca ttattcatgg
     6181 catagcgatg gtcgcaatat cctctacgcg ggtgaagttg tgcaaaaccc tcccggcaca
     6241 cctcccgtcg atgtcgcctc gttcaaggca tggggtgatt ttgccgcaga taaacacagt
     6301 ctctattttg aaggcaagcg caccgatgac aatggtggtg gaaatagctt ggatatcaaa
     6361 acgctgcatc aggtggaatt ccggcctccc tgggatcctg acttactggg gctgatatta
     6421 cgtgacgcta atttcttgta tatcaatggg catcgcctcg ctgaccccga gagtttccgt
     6481 gtgctggcgc agaagtcctg ggatcagcga ggaaagtttt ccaccacgtt taacccctgc
     6541 attgccgtac catttggccc ctgggatacc ctggctcgta cacggaccaa aatcctgctc
     6601 aacggcgaac agcttgatgc cgacccggac accttttccg tcgtgcgctg gatgcccggc
     6661 tcactcttga cctggcgtga taaaaacggg ctgcagcgta aagtcctcga caaggaaaat
     6721 ctggcgtggg atgaagattt aacaaagcac tgtctggatt tttctctgct ggaaaagaaa
     6781 gtgttctggc gtaaaggacc tgcttgtaaa caggaagaat tacccggact cgatccggag
     6841 cagtttcaac ccatcagtga tgctgtcgcc cagcatcagg actcgcttta taccatcatc
     6901 gaaacagagt ctggtgaccg taagctggag atcgtgaaac ttgatgatcc caatcttatt
     6961 atcaacaaac gtttcaacgc cgcgaaacgc cacggctatt tacttacgcg tgccgaaggg
     7021 tagccatacc attccggttt acacgtgttt gaatctgacg gaccgctgat cttactggat
     7081 aaccgttctc cggatgaacg cgaagcccat cttaatgacc atcccttttt gcgcagatgg
     7141 tatgcccgcg ataaccgcta catttacagc tttgatggcg cgcagctctg gcgataccgc
     7201 accgctgatc cgaaacaagt tcgcttaatc tggaaggaac aacattcggg atatgtctat
     7261 ggcgtaaatt acaaaacggg atatctggac ggaaaaatta ctgatgacgg cgaatttatt
     7321 cctgccccgc gcaatgaggc gacaaaatga agggtcaaat aatcacagtt gcactcatcc
     7381 tgttgggaca cacatttctg acgcctgctg tacaggccat agggttcgac tattacaatg
     7441 atcacggtgt tatgtcttac ggtaaaggtt attgggggga taagaaaatc attgcccagt
     7501 ttccggagat gagcagggcc ggtttgcgcc ttgtgaaaaa tatctctggg aaaacgggtt
     7561 atataccttc cgatgaaata agcatagaaa atgaggaata ccactgggtg acagacggcc
     7621 gcgttattct ctggcgtggc aaaatcgtta gcaatccacc gggaacactc actgtcgata
     7681 ttgccagctt tcaggctatg ggccgcttcg cggtcgataa atatagcctc tattttgacg
     7741 gacagcgcac cgaaagtaat agcggcgcgt cacgtgttga gctggcgaca ctaaaagcta
     7801 tcgaaggtaa ctctaccacg ctggtggata gtaaaaatct ctacttgtcc ggtcgacgtc
     7861 aggggagcag cagtgatgtt actgtattag aaaaaagatg gtggggtatt aatccacgtc
     7921 ttatgagtgt aaacagaaat tcatattcca atgatctgct tatccgcagt gggcagaata
     7981 tttatttaaa tggcgttcac cttacggcga atgcagactc atttgagata attcgctgga
     8041 tacctcattc actgctggtt tttcgcgaca ataagggtct gcatcgttat ccctttggtc
     8101 aattatcagg caaagcgata cccgtagatg atgacgtctc ttttgaagta ggggaaagtc
     8161 gcgttcgctg gcgtaaacag ctcacgcccg accgtcagtg gagcaagtgg atagacctac
     8221 caggtattg
//
LOCUS       DAJVPP010000149         8178 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVPP010000149 DAJVPP010000000
VERSION     DAJVPP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.149"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(758..1954)
                     /gene="hemY"
                     /locus_tag="OQM34_003684"
     CDS             complement(758..1954)
                     /gene="hemY"
                     /locus_tag="OQM34_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1453950.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1957..3186)
                     /gene="hemX"
                     /locus_tag="OQM34_003685"
     CDS             complement(1957..3186)
                     /gene="hemX"
                     /locus_tag="OQM34_003685"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1453951.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            complement(3208..3948)
                     /gene="hemD"
                     /locus_tag="OQM34_003686"
     CDS             complement(3208..3948)
                     /gene="hemD"
                     /locus_tag="OQM34_003686"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1453952.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3945..4886)
                     /gene="hemC"
                     /locus_tag="OQM34_003687"
     CDS             complement(3945..4886)
                     /gene="hemC"
                     /locus_tag="OQM34_003687"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1453953.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5273..7819
                     /gene="cyaA"
                     /locus_tag="OQM34_003688"
     CDS             5273..7819
                     /gene="cyaA"
                     /locus_tag="OQM34_003688"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1453954.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7859..>8178)
                     /gene="cyaY"
                     /locus_tag="OQM34_003689"
     CDS             complement(7859..>8178)
                     /gene="cyaY"
                     /locus_tag="OQM34_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1453955.1"
                     /translation="NDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFEN
                     GSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGETV
                     SFR"
BASE COUNT         1859 a         2135 c         2110 g         2074 t
ORIGIN      
        1 tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca agacgtttgg
       61 gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat gatgacgtaa
      121 ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa taatagtgac
      181 aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag cgttttcaat
      241 cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg attattgatg
      301 ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa ttttatcatt
      361 cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa gttattactc
      421 aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg ttatttcccg
      481 attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt cgccttagtg
      541 cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca ctgacagatg
      601 tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc acttgttgtc
      661 atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt atgcatcaga
      721 aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg ttattctgca
      781 acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg tgcagtctgt
      841 ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc agcgccgcgc
      901 ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga cccaatgtgc
      961 tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact ttttccagct
     1021 gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg tcatactggc
     1081 gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat tcaataagat
     1141 gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt tggtttttcc
     1201 accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc aggccaatcc
     1261 atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct ttcgccattg
     1321 atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc tgttccgcca
     1381 gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatccacg ccgtggcgtg
     1441 cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct accggaatgg
     1501 tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt gcttcatcac
     1561 cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt tcagcgtgat
     1621 cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc agtttcagca
     1681 gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac catccacggg
     1741 tgtgcgcgcc agtgcggaag atccgccgta gtagccactc aatggcaaac agcactacca
     1801 tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg tagttgtcgg
     1861 tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact atccccgcaa
     1921 tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg gcgcggctgc
     1981 cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggtgca ggtgcaggtt tagcttccgt tgtccccgct gccggttgtg ccagcaggtt
     2101 acgcacgcga gtctgcatca gtttttccag catcgcctgg ctttgcaggg tttccggaag
     2161 atccatcgag atattttgct ggcttaactg gtccacctcg tcgaggaacg ctttggtggt
     2221 ggcatcatca gtatcgtagt aagcacgtac ccaggtggag acgttctcca gcgcctggcg
     2281 ataagtctct tcctgatgac gcggtacagc ttgtgctgcg accagcaggc gagagcgaat
     2341 attttcgcgc agatagatat cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg
     2401 acggcgaatc gtaatgaagt tgtccataaa gttctgccag cttttttgca gattgatacg
     2461 ccattcgctg atggaactgg aaagctcttc accgtctgaa tccatcggcg aaccatcgct
     2521 gtcattatcg gccagacgca ggttatctac ctgatttgaa agctgattaa gcttaaggat
     2581 gatgccgtca taatccacct gcgatactgc agaaaggctg gcgatatcat cggtaattgc
     2641 ccgacgaacg gtaatcagac tcgggtcatt catatccgcc aggctggcgt ctgcactttt
     2701 cagcaacgct gcagaggtcg tgacgtcctg atcgctccac agcttccgtc cggcgagttt
     2761 caccagaaaa tcggcctgag ccagcagcca ggttttagca tcgctgccgg aaatggtggc
     2821 gatcttttgt tggacttcat ccaactgttt tgccagcgtt tcttgctgac gattcgcctg
     2881 cttaagctgc gcagcttgtt gcttaataat gccttccagc tcggcttttt ggctctcctg
     2941 ggctttttgc aatgccgtca gttggttagc cagggcatcg ctggtggcgg tctgattgac
     3001 ggcctgttgt ttaccccagc catacaaacc gacacccgcc gccagagcaa tagcgatagc
     3061 caccgcgctg agaatcaatg cagtattgtt cttactcttt ttttctgttg cgacaggttg
     3121 tgacgtggtg tccacggcct ccctggtctc ttcaaccacg gcggaggttt tttcttgttc
     3181 cgtcattatg gcttcctgtt atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc
     3241 agcgttatcg gcgaccttaa tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg
     3301 ctcactgacg accaatagtc gacagtgtag taaccagtgc tcacgatacc attgtgggat
     3361 cagcgaccag agttgctgca acatttcacc gctggtaaca acgaccatcg tcacctcgcg
     3421 ggattgccag cgcatcgctt cttctgcacc atcgtaatgg attgcgcatc gttgataaca
     3481 ttcacaaaaa gtgacctcag caccgcgcac cgtcagggta tccccaatta gctcacgacc
     3541 gccattgcca cgtaatatca gcgcacgttt gcccgcaata ttttgtaatt caggtaattg
     3601 tagcaagact tcgctgattt cccgatcctg tgggtagaga atcttctgtc cgcttacggt
     3661 atgtagcgcc agtgcggtgg tgcgtccaat ggcgaaataa gtaggtagtt ggggccattt
     3721 acgatcttgc tgatgcagct gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa
     3781 caacagatcg ctctccccca gcgctgccag ttgatcagca agttgcggta attgtcgacc
     3841 cggagaaaac tcaatcagcg gaaaatgcca ggccacctgc cccagtgtgc gcagacggct
     3901 cactaactct tctccagcgg gagacgggcg ggtgaccagg atactcatgc cggggcctct
     3961 ccgttataga cttcagcgag gatctcgcgc gcgccgttat tcagtagctc ttctgccagc
     4021 gaaatcccca tttgttcggc atcttgcggc gcaccgcggc gttcaccgcg aataatctgc
     4081 gaaccgtccg gcgcgccgac cagcgcacat agccagattt cgccatcaat aagctcggcg
     4141 tagctaccaa ttggcacctg acatccgcct tcgagacggg tattcatggc gcgttctgcg
     4201 gtaacgcgca gtgcagtttc gtggtgattc agcgcggcaa gcagctcgcg agtgcgtgta
     4261 tcatcaaggc ggcattcaat accgactgca ccttgtccta ccgccggaag agaaatctcg
     4321 ggtggtaacg cggcgcgaat gcgggactcc agacctaaac gttttagtcc ggctacggca
     4381 agaatgatgg catcgtattc gccgttatct agtttgctca ggcgagtgcc gacgttgccg
     4441 cgcagggagc ggataatcag atccgggcgg cgttcagcca gttggcactg gcgacgtaaa
     4501 ctggacgtcc cgacgatact gcctgctggt aacgcatcca gactgtcata gttattggac
     4561 acaaaggcat cgcgaggatc ttcacgctcg caaatagtga ccagccccag accttgcggg
     4621 aattcaaccg gcacatcttt cattgaatgt acggcgatat cagcgcgatt ctccaggagc
     4681 gcgacttcca gctcttttac aaataagcct tttccgccta ctttcgccag cggcgtatca
     4741 agaatcacat cgccgcgcgt caccatcggt accagttcaa cgaccaggcc cggatggctc
     4801 gccatcaact tgtctttgac atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg
     4861 gcaattctta aaacattgtc taacatgctt gttaccgtca ttatcatccg tggtccatcc
     4921 taacatcctt gccagagtga tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg
     4981 taaaatgagg tctggcagtg gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt
     5041 acagagaata aacggtgcta cacttgtatg tagcgcatct ttctttacgg tcaatcagca
     5101 aggtgttaaa ttgatcacgt tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc
     5161 gcgaaaagtg gtaacggtta cctttgacat acgaaatatc ccgaatgccg cgtgttaccg
     5221 ttgatgttgg cggaatcaca gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct
     5281 ctatattgag actctgaaac agagactgga tgccataaat caattgcgtg tggatcgcgc
     5341 gcttgctgct atggggcctg cattccaaca ggtctacagt ctactgccga cattgttgca
     5401 ctatcaccat ccgctaatgc cgggttacct tgatggtaac gttcccaaag gcatttgcct
     5461 ttacacgcct gatgaaactc aacgccacta cctgaacgag cttgaactgt atcgtggaat
     5521 gtcagtacag gatccaccga aaggtgagct tccaattact ggtgtataca ccatgggcag
     5581 cacctcgtcc gtagggcaaa gttgttcctc tgacctggat atctgggtct gtcatcaatc
     5641 ctggctcgat agcgaagagc gccaattact acaacgtaaa tgcagcctgc tggaaagctg
     5701 ggccgcctcg ctgggtgtgg aagtcagctt cttcctgatt gatgaaaacc gcttccgtca
     5761 taatgaaagc ggcagcctgg ggggcgaaga ttgtggctcc acccagcata tactgctgct
     5821 tgacgaattt tatcgtactg ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt
     5881 gccgtgcgac gaagaagagc attacgacga ctatgtgatg acgctttacg cgcagggcgt
     5941 gctgacgcca aatgaatggc tggatctcgg tggcttaagc tcgctttctg ctgaagagta
     6001 ctttggtgcc agcctttggc agctctacaa gagtatcgat tccccataca aagcggtgct
     6061 gaaaacactg ctgctggaag cctattcctg ggaatacccg aacccacgtc tgctggcgaa
     6121 agatatcaaa cagcgtttgc acgacggcga gattgtatcg tttggcctcg atccatactg
     6181 catgatgctg gagcgtgtta ctgaatacct gacggcgatt gaagatttca cccgtctgga
     6241 tttagtacgt cgctgcttct atttaaaagt gtgcgaaaaa ctcagccgtg aacgcgcctg
     6301 tgtaggctgg cgtcgcgcag tgttgagcca gttagtgagc gagtggggtt gggacgaagc
     6361 tcgtctggca atgctcgata accgtgctaa ctggaagatt gatcaggtgc gtgaggcgca
     6421 caacgagctg ctcgacgcga tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg
     6481 caataacctt agcgtctccg ccagtccgca ggatatcggc gtactgacgc gtaaactgta
     6541 tgccgcgttt gaagcattgc caggtaaagt gacgctggta aacccgcaga tttcacccga
     6601 tctctcggaa ccgaatctga cctttattta tgtgccgccg ggccgggcta accgttcagg
     6661 ttggtatctg tataaccgcg cgccaaatat tgagtcgatc atcagccatc agccgctgga
     6721 atataaccgt tacctgaata aactggtggc gtgggcatgg tttaacggcc tgctgacctc
     6781 gcgcacccgt ttgtatatta aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat
     6841 ggtcgccgac gtgtcgcacc atttcccgct gcgcttacct gcaccgacac cgaaggcgct
     6901 ctacagcccg tgtgagattc gccatctggc gattatcgtt aacctggaat atgacccgac
     6961 agctgcgttc cgcaatcagg tggtgcattt tgatttccgt aagctggatg tcttcagctt
     7021 tggcgagaat caaaattgcc tggtaggtag cgttgacctg ctgtaccgca actcgtggaa
     7081 cgaagtgcgt acgctgcact tcaacggcga gcaatcgatg atcgaagccc tgaaaactat
     7141 tctcggcaaa atgcatcagg acgccgcacc gccagatagc gtggaagtct tctgttatag
     7201 ccagcatctg cgcggcttaa tccgtactcg cgtgcagcaa ctggtttctg agtgtattga
     7261 actgcgcctt tccagcaccc gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg
     7321 tcaaacctgg ggcttgttct tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc
     7381 catcgagttt tatggcgcta tttcgcataa caaactgcac ggcttgtcag tgcaggttga
     7441 aaccaatcac gtcaaattac cggcggtggt ggacggcttt gccagcgaag ggatcatcca
     7501 gttctttttc gaagaaacgc aagacgagaa tggctttaat atctacattc tcgacgaaag
     7561 caaccgggtt gaggtgtatc accactgcga aggcagcaaa gaggagctgg tacgtgacgt
     7621 cagtcgcttc tactcgtcat cgcacgaccg cttcacctac ggctcaagct tcatcaactt
     7681 caacctgccg cagttctatc agattgtgaa ggttgatggt cgcgaacagg tgattccgtt
     7741 ccgaacaaaa tcgattggca acctgccgcc cgccaatcag gatcacgata cgccgctatt
     7801 acagcagtat ttttcgtgat gagcgtgccg gaaagcgagg cttatccggc atgcaatctt
     7861 agcggaaact gactgtttca cccgcctgtt gcgtcgccgc ctgttccagc aaatcccaga
     7921 aggtttcgcc gctgcgatcg caaatccatt catcgccttt caggtcaaaa tggtagccgc
     7981 cctgtttggt tgccagccac acctggtgca gcggctcctg gcggttaata atgattttgc
     8041 tgccgttttc aaaggtaatg gtcagtacgc cgccgttgat ttcgcagtcg atatcgctgt
     8101 cgccatccct gtcgtccaga cgctcttcaa tggtcagcca cagttgatca gccaggcgat
     8161 gaaattcact gtcgttca
//
LOCUS       DAJVPP010000150         8032 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVPP010000150 DAJVPP010000000
VERSION     DAJVPP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.150"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..92
                     /gene="hokE"
                     /locus_tag="OQM34_003690"
     CDS             <1..92
                     /gene="hokE"
                     /locus_tag="OQM34_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1453956.1"
                     /translation="LLVGDSLCEFTVKERNIEFKAVLAYEPKK"
     gene            complement(214..834)
                     /gene="entD"
                     /locus_tag="OQM34_003691"
     CDS             complement(214..834)
                     /gene="entD"
                     /locus_tag="OQM34_003691"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCS1453957.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1009..3249)
                     /gene="fepA"
                     /locus_tag="OQM34_003692"
     CDS             complement(1009..3249)
                     /gene="fepA"
                     /locus_tag="OQM34_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCS1453958.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKNENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3492..4694
                     /gene="fes"
                     /locus_tag="OQM34_003693"
     CDS             3492..4694
                     /gene="fes"
                     /locus_tag="OQM34_003693"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCS1453959.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4697..4915
                     /gene="ybdZ"
                     /locus_tag="OQM34_003694"
     CDS             4697..4915
                     /gene="ybdZ"
                     /locus_tag="OQM34_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCS1453960.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            4912..>8032
                     /locus_tag="OQM34_003695"
     CDS             4912..>8032
                     /locus_tag="OQM34_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit F"
                     /protein_id="HCS1453961.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQVQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLAT"
BASE COUNT         1733 a         2150 c         2204 g         1945 t
ORIGIN      
        1 cgcttctggt cggagactcg ctgtgtgagt tcaccgtaaa ggaacgtaat attgagttta
       61 aggctgttct tgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata
      121 tccggttgtc aggtcagagt ggtaaggcac ctgctttaca ctttcgcccg cagtcagtga
      181 tggctgcggg cgaatcgtac cagatgttgt caattaatcg tgttggcaca gagttatgac
      241 tattttttct tttatctgcc agtgcacagc aaacatctca ttctcacgat gaatgatgac
      301 ctgctgttta ttccagctaa ttatctgata gtccagaaaa cctgcatcag tttggatctc
      361 acttgcctta aatgcgctct ctttggcgga aaatgcaagt gtcagcgcca gagaaaaggc
      421 taaaccgcag tctgcgagtc gctcgtgttc tgctggtgta ataatgttgt ctgtcaattc
      481 ggtcgctgtt tgcgcagaaa aaatttcttc gatatcaacg ccaatcggtt gacgggaaac
      541 cacggccaat gccgtagccc cgcagtggct aatactgccg tatacctccg caggccacac
      601 cggttggcgt agctcgccga ttgcgggcac acatttatag ccatattccc gcaaggcata
      661 aacagcagcg atccgtccgg ctaaatgctc tgttttacgt ttacgtccag cgtgttgcag
      721 ttgtgcgtag tgcggcagcc agagtaaatc ctgctcacaa aaattcgccg gatcgaactc
      781 aacaaaatgc agcgtatgtc cggcaaaggg gagggaggta tgcgtagttt tcatatcgac
      841 catattcgag actgatgaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
      901 gtgatcctgc aatatattga attggcaaga tctttgtagg ccggataagg cgttcacgcc
      961 gcatccggcg tgaacgacgc gcactttgtc aacaatctga cgttagcatc agaagtgagt
     1021 attaatgctc atataccacg tacgtcccgg ctcgttatag gtatacgcgc cggcaccggc
     1081 gatatagttg gctcctgcca gatcgcccgt ggtctgggca ttacctgcac gccacaaacg
     1141 tttgtcgaac aggttgtcca cgccgccggt cagactgaca ttcttcgtca catcccaggt
     1201 cgcgctcaga cccacaatgc tgtaaggact gatttctttg gtttccgggc caaccgctgg
     1261 ctgaccttta tagttgtact tcttcggctg ctgcttgccg taccaggtga aggtcgtttg
     1321 catcgacaaa tcttcccgtg cctgccagct cagcgttgag ttcaacgtgt actccgggat
     1381 gatcgacaaa cggtcgcccg tggttttgtt ttcattcttc agcatataag tgatgttatt
     1441 ggtccacatc accgtttcgc taactggtac gtttaacgat ccttccagac cttcaaccac
     1501 tgctttcggc acgttatccc actgatagag atcagtgccg actgcgtttt gccctacagc
     1561 cacatagccc gcttcaatct tattgcgata atcgttacgg aaccaggtga cgcccgccag
     1621 ccagccgtcg cgtttgaact ccagaccaat ctctttgttg atgctggttt ctactttcag
     1681 gtcatcgtta ccttgcagat agcagccgcc cgcgctggca tagcagccct gacctttact
     1741 gtagagaatg tagttcgggt tagtctggta taggctcggc gctttatagg cgcgggcaat
     1801 gcctattttc agcgtgaagt catcgcctaa accttgcgat atgttcagcg ccgggctcca
     1861 gttattgccg acaatactgt gatgatcgaa acgcagcccc ggcgttacga tggtgctgtc
     1921 agtcagctcc atgttgtttt cggcaaacag cgagaaaatt tctgcttttg aatacgggct
     1981 acggtcggtg gcactcacgc catcaatagc gccaccggta ttcgttccgg tcagtgcttg
     2041 ggtgttggaa cttaagtcct tcatccgttg ctgattccac tccgtaccca gcgtcagcgt
     2101 ctggttaacg aggaaatcaa tcggcaggtt aacttcgctg tgcagcatca cgtcatcgag
     2161 atcgatatcg acgaaatcct gtgtcgcttt ttcgttaaat ttcccttcgg taccgcccgc
     2221 cagaccttcc ggaatacgcg agttacgggt gtgttcatac tgcacccagt tgctggtggt
     2281 cacgccgtta tcccagccac cgttccaggt cagcgcgtag ttctggcgat acaggcggtt
     2341 ggtttcatcg ccatatttcg agcgagtgta agcgtcagag ttggtgttct gggtatcgcc
     2401 cgcatacagg ttaccctggc ggctgtaacc cgcttccagt tccagcgatt gcaatggtgc
     2461 gaagtcccag cgcaccacgc cgttaatatc tttattgatc accccttcgc gcccggctgg
     2521 taacgtcgtg gcatacgttc cggcacgcgc ggactgatgg ccctggttga tatcccacgc
     2581 gtcagcctga gttttgtcga ggttgccata caagcggaag ctgaattcgt cgcccagcgg
     2641 accggtcagg ctaaagttag tgcgtttggt ggcaccttcc tctttatgtt ctggcgcatt
     2701 gaaatatgcg tcccaggagc cgtgccactc gccgctgcct tttttggtaa tgatgttaac
     2761 cacgccgccc gccgcgccgt tgccataacg cgcagctgcc ggaccacgca gaacttcaat
     2821 acgttcaatc atttcaggtg gcacccagga ggtatcacca cgggtatcgc gctcgccacg
     2881 ccagccctga cgcaccgagt tacggctgct taccggcttg ccgtcaatca aaatcagcgt
     2941 gttttccgga cccataccgc gaatatcaat ctgtcggtta ttcccacgct gaccactggt
     3001 ggagttaccg gtcaggttaa cgcctggcat ggtacggatg atctccgaca catcgcgggc
     3061 aaccgggttt ttgcggattt catctgcggt gatggtcgaa acgccaggag cctgcaagtt
     3121 ctgctcggcg gcggtaacga caatggtatc gtcatgtgaa acaggtgtat cggtcggctc
     3181 ttgtgcctgc gctaccccat aaatccccag attgaccaac aaggccaggg aatgaatctt
     3241 cttgttcatt gttttattcc tgcatttttg ccacgaattg caactgtcgg gcatggtcgt
     3301 catcaacacg acgcatcccg ttaccgcgaa aacctttgat cctgaaagac acgcagtgca
     3361 gttggttaat taatgtccgc gcttcccacg gcgcgccatt acgctattgc aaatgcaaat
     3421 agttattaat aacattatca atatatttct gcaatcaatg aaaaattgca cagtaaacat
     3481 ggggttatgg tgtgacggcg ttaaaagtag gaagtgagag ctggtggcag tcgaaacatg
     3541 gcccggaatg gcagcgtctg aatgacgaaa tgtttgaggt cactttctgg tggcgtgatc
     3601 cccaaggttc tgaagaatac tcgacgataa agcgcgtatg ggtctacatc actggtgtga
     3661 ccgatcatca tcagaacagc cagccccagt cgatgcagcg aattgcaggt actgatgtct
     3721 ggcagtggac gacacaactc aatgccaact ggcgcggcag ctactgcttt attcccaccg
     3781 aacgcgatga cattttttct gcaccatccc ccgatcgcct cgaattacgt gaaggctggc
     3841 gaaaactatt accccaggca atagccgatc cgctgaatcc acaaagctgg aaaggcgggc
     3901 tagggcacgc tgtttctgca ctcgaaatgc cgcaagcgcc tctgcaaccg ggatgggatt
     3961 gtccgcaagc gccagaaata ccagccaaag aaattatctg gaaaagtgaa cggttgaaaa
     4021 attcacgtcg tgtatggatt tttaccaccg gcgatgtaac agcagaagaa cgcccgctgg
     4081 cagttttgct cgatggcgaa ttttgggcgc aaagtatgcc cgtctggcct gtgctgactt
     4141 cgctgaccca tcgtcagcaa cttcctcccg ccgtgtatgt gttgatcgac gctatcgaca
     4201 ccacgcaccg cgcccacgaa ctgccgtgta atgcggattt ctggctggca gtacagcaag
     4261 agttattacc cctggtgaaa gttattgccc cttttagcga tcgcgccgat cgcaccgtgg
     4321 tcgccgggca gagttttggt gggctgtccg cgctgtatgc cggactgcac tggcctgaac
     4381 gctttggctg tgtattaagc cagtcaggat cgtactggtg gccgcatcgg ggcgggcagc
     4441 aagagggcgt gttacttgaa aagctaaaag ctggtgaagt tagcgccgaa ggtctgcgca
     4501 ttgtgctgga agcgggtatt cgcgagccga tgatcatgcg ggccaatcag gcgctgtatg
     4561 cgcaattaca ccctataaaa gaatccattt tctggcgtca ggttgacggc ggacatgatg
     4621 cgctttgttg gcgcggtggc ttgatgcagg ggttaatcga cctctggcaa ccacttttcc
     4681 atgacaggag ttgaatatgg cattcagtaa tcccttcgat gatccacagg gagcgtttta
     4741 cattttgcgc aatgcgcagg ggcaattcag tctgtggccg caacaatgcg ccttaccggc
     4801 aggctgggac attgtgtgtc agccgcagtc acaagagtcc tgccagcagt ggcttgaagc
     4861 ccactggcgt actctgacac cggcgaattt cacccagttg caggaggcac aatgagccag
     4921 catttacctt tggtcgccgc acagcccggc atctggatgg cagaaaaact gtcggattta
     4981 ccctccgcct ggagcgtggc gcattacgtt gagttaaccg gagaggttga tgcgccatta
     5041 ctggcccgcg cggtggttgc cggattagcg caagcagata ctttgcggat gcgttttacg
     5101 gaagataacg gcgaagtctg gcaatgggtc gatgatgcgc tgatattcga actgccagaa
     5161 attatcgacc tgcgaaccaa tattgatccg cacggtactg cgcaggcatt aatgctggcg
     5221 gatttgcaac aagatttgcg cgtcgatagc ggtaaaccac tggtctttca tcaactgata
     5281 caggtggcag ataaccgctg gtactggtat cagcgttatc accatttact ggtcgatggc
     5341 ttcagttttc cggccattac ccgccagatc gccaacattt actgcgcatt gctgcgtggc
     5401 gaaccaacgc ctgcttcgcc gtttacgcct ttcgctgatg tagtggaaga gtaccagcaa
     5461 taccgcgaaa gcgaagcctg gcagcgtgat gcggcattct gggtggaaca gcgtcgccaa
     5521 ctgccaccgc ccgcgtcact ttctccggca cctttagcgg ggcgcagcgc ctcggcagat
     5581 attttgcgtc tgaaaatgga atttaccgac ggggaattcc gccagctggc tacgcaactt
     5641 tcaggtgtgc agcgtaccga tttagccctt gcgctgacgg ctttgtggct ggggcgattg
     5701 tgcaatcgca tggactacgc cgccggattt atctttatgc gtcgactggg ctcggcggcg
     5761 ctgacggcta ccggacccgt gctcaacgtt ttgccgctgg gtattcatat tgcggcgcaa
     5821 gaaacgctgc cggaactggc aacccgactg gcagcacaac tgaaaaaaat gcgtcgtcat
     5881 caacgttacg atgccgaaca aattgtccgt gacagcgggc gagcggcagg tgatgaaccg
     5941 ctgtttggtc cggtactcaa tattaaggta tttgattacc aactggatat tcctggtgtt
     6001 caggtgcaaa cccataccct ggcaaccggt ccggttaatg accttgaact ggccctgttc
     6061 ccggatgaac acggtgattt gagtattgag atcctcgcca ataaacagcg ttacgatgag
     6121 ccaacgttaa tccagcatgc tgaacgcctg aaaatgctga ttgcccagtt cgccgcggat
     6181 ccggcgctgt tgtgcggcga tgtcgatatt atgctgccag gtgagtatgc gcagctggcg
     6241 cagatcaacg ccactcaggt tgagattcca gaaaccacgc tgagcgcgct ggtggcagaa
     6301 caagcggcaa aaacaccgga tgctccggcg ctggcagatg cgcgttacct gttcagctat
     6361 cgggaaatgc acgagcaggt ggtggcactg gcgaatctgc tgcgtgagcg cggcgttaaa
     6421 ccaggggaca gcgtggcggt ggcactaccg cgctcggtct ttttgaccct ggcactccat
     6481 gcaatagttg aagctggcgc ggcctggcta ccgctggata ccggctatcc ggacgatcgc
     6541 ctgaaaatga tgctggaaga tgcgcgtccg tcgctgttaa ttaccaccga cgatcaactg
     6601 ccgcgcttta gcgatgttcc caatttaacc aacctttgct ataacgcccc gcttaccccc
     6661 cagggcagtg cgccgctgca actttcacaa ccgcaccaca cggcttatat catctttacc
     6721 tctggctcca ccggcaggcc gaaaggggta atggtcgggc agacggctat cgtcaaccgc
     6781 ctgttgtgga tgcaaaatca ttatccgctc acaggcgaag atgtcgttgc ccaaaaaacg
     6841 ccgtgcagtt ttgatgtctc ggtgtgggag tttttctggc cgtttattgc cggggctaaa
     6901 ctggtgatgg ctgaaccgga agcgcaccgc gacccgctcg ctatgcagca attctttgcc
     6961 gaatatggcg taacgaccac gcactttgtg ccgtcgatgc tggcagcatt tgttgcctcg
     7021 ctgactccgc aaaccgctcg ccagagttgc gtgacgttga aacaggtttt ctgtagtggt
     7081 gaggccttac cggctgattt atgccgcgaa tggcaacagt taaccggcgc gccgttgcat
     7141 aacctgtatg gcccgacgga agcggcggta gatgtcagct ggtatccggc ttttggcgag
     7201 gaactggcac aggtgcgcgg cagcagtgtg ccgattggtt atccggtatg gaatacgggt
     7261 ctgcgtattc ttgatgcgat gatgcatccg gtgccgccgg gtgtggcggg tgatctctat
     7321 ctcaccggta ttcaactggc gcaggggtat cttggacgac ccgatctgac cgccagccgc
     7381 tttattgccg atccttttgc tcctggtgaa cggatgtatc gtaccggaga cgttgcccgc
     7441 tggctggata acggcgcggt ggagtacctc gggcgcagtg acgatcagct aaaaattcgc
     7501 gggcagcgta tcgaactggg cgaaatcgat cgcgtgatgc aggcgctgcc ggatgtcaaa
     7561 caagccgtta cccacgcctg tgtgattaac caggcggcag ccactggtgg tgatgcgcgt
     7621 cagttggtgg gctatctggt atcgcaatcg ggcctgccgt tggataccag cgcattgcag
     7681 gcgcagcttc gcgaaacatt gccgccgcat atggtgccag tcgttctgct gcaactgccg
     7741 cagttgccac ttagcgccaa cggcaagctg gatcgcaaag ccttaccgtt gcctgaactg
     7801 aaggcacaag cgccaggacg tgcgccgaaa gcgggcagtg aaacgattat cgccgcggca
     7861 ttcgcgtcgt tgctgggttg tgacgtgcag gatgccgatg ctgatttctt tgcgctgggc
     7921 ggtcattcgc tactggcaat gaaactggca gcgcagttaa gtcgacagtt tgctcgtcag
     7981 gtgacgccgg ggcaggtgat ggtcgcgtca accgtcgcca aactggcaac ga
//
LOCUS       DAJVPP010000151         7973 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVPP010000151 DAJVPP010000000
VERSION     DAJVPP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.151"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            25..1953
                     /locus_tag="OQM34_003696"
     CDS             25..1953
                     /locus_tag="OQM34_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCS1453962.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            1937..2140
                     /locus_tag="OQM34_003697"
     CDS             1937..2140
                     /locus_tag="OQM34_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1453963.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2137..3730
                     /locus_tag="OQM34_003698"
                     /pseudo
     CDS             2137..3730
                     /locus_tag="OQM34_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3720..5225
                     /locus_tag="OQM34_003699"
     CDS             3720..5225
                     /locus_tag="OQM34_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCS1453964.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASDRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5262..5609
                     /locus_tag="OQM34_003700"
     CDS             5262..5609
                     /locus_tag="OQM34_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1453965.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5667..6695
                     /locus_tag="OQM34_003701"
     CDS             5667..6695
                     /locus_tag="OQM34_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1453966.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6747..7133
                     /locus_tag="OQM34_003702"
     CDS             6747..7133
                     /locus_tag="OQM34_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1453967.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7145..7522
                     /locus_tag="OQM34_003703"
     CDS             7145..7522
                     /locus_tag="OQM34_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1453968.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7509..>7973
                     /locus_tag="OQM34_003704"
     CDS             7509..>7973
                     /locus_tag="OQM34_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1453969.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSVWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1838 a         1875 c         2484 g         1776 t
ORIGIN      
        1 gggataaatt accggattta ctcgatgaat atatcagaac aacaactgag taatatgatg
       61 gtggcagtca ctatcgcact gcagccactg gtcagggtat tgccggtgac ggctgtagaa
      121 tgggccgatc aaaattatta cctgcctaaa gaatcgtcat acggtgaggg ggaatggaaa
      181 acgctgccgt ttcaggtcgc catcatgaac tgcatgggca atgacgagat tcgtacagtt
      241 aacctgatta aatctgctcg tgttggttat acaaaaatgg tgcttggtgt gatcgggtat
      301 tttattgagc acaaatcccg caacactctt ctttttcagc cgacggactc agctgctgaa
      361 gattttatga agtctcacgt ggaagcaaca attcgtgatg ttccttgcct gaaaaaactt
      421 tctccctggc tgggccggaa acaccgggat aatacgctca cactgaagcg tttttcctct
      481 ggtgtggggt tctggtgttt gggtggtgct gctgccaaaa actaccgtga aaaatctgtc
      541 gatgtggtct gctatgacga actctcctcg tttgaaccgg atgtggaaaa agaaggttcg
      601 ccaacactgc ttggcgataa acgtattgaa ggttcggtat ggccaaaatc catacgcggt
      661 tcaacgccaa aaattaaagg ctcctgtcag attgaaaaag ctgcgaatga atctgcgcat
      721 ttcatgcggt tttatgtccc ttgccctcat tgcggggagg agcagtatct gaagtttggc
      781 gatgatgcga cgtcgtttgg cctgaaatgg gagaagggta aaccggaaac ggtgtattac
      841 ctgtgtgaac ataatggctg cgtgatccgt cagtctgaac ttgatcagac cggggggcgc
      901 tggatttgtg acaataccgg gatgtggacg cgtgacggtc tgacgttttt cagtgcttcg
      961 ggtaatgaaa ttccgccgcc gcgctccatc actttccata tatggacggc gtacagtccg
     1021 ttcaccacct gggtacagat agtctgtgac tggctggatg cactgaaaga tcccaacggc
     1081 gtgaaaactt ttgtgaacac cacgctgggc gagacctggg aagaggccgt gggcgaaaaa
     1141 ctcgatcacc aggtactgat ggataaggtt gtgccttaca cggcaacggt gcctgtccgg
     1201 gtggtttatc tgacggcggg cattgactcg cagcgaaacc gttttgagat gtatgtctgg
     1261 gggtgggctc cgggagagga agcctttctg gtggataaaa tcatcatcat gggacgtcct
     1321 gatgaggaag agacgctgtt acgtgtggat gcggcgatca ataaaaaata ccgccatgca
     1381 gacggtaccg aaatgaccat ttcccgtgtc tgctgggata tcggggggat cgatggcgaa
     1441 atcgtgtatc agaggtcaaa aaaacacggt gttttccggg tactgccggt aaaaggtgcc
     1501 tctgtctatg gcaagccggt gatcaccatg ccgaaaaccc gcaatcagcg gggtgtgtat
     1561 ctgtgtgagg tggggacgga taccgcaaaa gaaattctct atgcccgaat gaaggccgat
     1621 cccacccctg tgaatgaggc cacatcgtat gccatccgct ttcctgataa tccggagatt
     1681 ttttcgcaga cagaggcaca acaactggtg gcggaggaac tggtggaaaa gtgggaaaaa
     1741 ggaaagatgc gtctgttgtg ggataacaaa aagcggcgta acgaagcgct ggactgcctg
     1801 gtgtatgcct atgcggcatt acgtgtgtcc gtgcaacgct ggcaacttga tctggctgtg
     1861 ctggcggcat cccgcaagga tgcaactgca caaccgaccc ttgaagaact ggcagcgaag
     1921 ctgtccggag gagtgaatgg ttacagtcgc tgaactgcag gcgctgcgtc aggcgcgcct
     1981 tgatttatta accggtaaac gggttgtgtc ggtacagaaa gacggtcgca ggatcgaata
     2041 tacggcagcg tccctgaatg agcttaaccg tgcgatcaac gatgcggagt cggttctggg
     2101 tacgacccga cgccgccgac cgctgggagt gaggttatga aacgaacgcc tgtcctgatt
     2161 gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acggaggcgg cgcaggattt
     2221 ggcgggcaaa tggcagagtg gttaccaccg gcgcagagtg ccgatgcggc cctgttgcct
     2281 gcattgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg tatcgcggct
     2341 aatgcggtgg ccctgcataa ggatcatatc gtcgggcaca tgtttcttat cagctaccgt
     2401 ctgaactggc gttggctggg gatgcgggag accacggcaa aaagttttgt cggggaggtg
     2461 gaagcagcct ggtcggaata cgcagaagga atgtttggcg agatcgacgt ggaagggaaa
     2521 cgcacgttta cggaatttat ccgtgaaggg gtgggcgtgc atgcctttaa cggcgaaatc
     2581 tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg ttttaaagcc
     2641 gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccactt tctgcgggcc
     2701 ggggttgagg tcgatcggta tgggcgggcg attgcgtacc atatctgtga ggatgatttt
     2761 ccgttctctg gtgccggacg ctgggagcgg atcccgcgtg agcttcccac cgggcgtccg
     2821 gccatgctgc atatttttga gccggtggag gacgggcaga cccgtggggc taatcagttt
     2881 tacagcgtaa tggagcggat gaagatgctg gactccctgc aggccacgca gcttcagtcg
     2941 gccattgtga aggcgatgta tgcagccaca atcgaaagtg aacttgatac cgaaaaggca
     3001 tttgaatata tcgcgggggc accgcaaggt cagcaggata atccgcttat taatattctg
     3061 gagaagttca cccgctggta tgacacgaat aacgtgacgc tgggcggtgt taaaattccg
     3121 cacctttttt cctggcgatg cgctgaacct gcagacagcg caggattcag acaatggatt
     3181 ttcggcgctt gaacaggcgc tgctgcggta tattgcagcc ggtcttggcg tttcctacga
     3241 acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct ccgccaatga
     3301 gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg ccacgcagat
     3361 gttttcctgc tggctggaag aggcgcttct tcgggggatt attcgtccgc cacgggcgcg
     3421 ttttgatttt taccaggcgc gttcagcctg gtcacgggca gagtggattg gctccggaag
     3481 aatggccatt gacgggctca aggaggtcca ggaatcggtg atgcgcattg aggccggact
     3541 gagcacgtat gagaaagaac tggcactgat gggcgaagat tatcaggaca ttttccgcca
     3601 gcaggtcagg gaatccgcag agcgcgaaaa agccggactc tcacgtccgg tgtggatagc
     3661 ccaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga caacatcacg
     3721 tgagacgtaa tctttcacat attatcgctg cggcattcaa tgagccgctg cttctggagc
     3781 ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgtga gatgggcgca gcaagccttt
     3841 cggtaccgca acagcaggta cagcttgacg ctcccgggat gctggctgaa acggacgagt
     3901 acatggccgg aggcaaacga cctgcccgtg tttaccgggt ggtgaacggt attgctgtac
     3961 tgccggtgac cggcacgctg gtacaccggc tgggtggcat gcggccattt tccggaatga
     4021 ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcggacact caggtacggg
     4081 gcgtactgct ggacattgac agtcctggcg ggcaggccac cggcgcgttt gactgcgctg
     4141 acatgattta ccgtctccgg cagctgaagc ctgtctgggc actgtgcaat gacacggcct
     4201 gttcagccgc catgttactg gcatcggcct gctcccgacg gctggttacc cagacatccc
     4261 gtatcggctc cattggggtg atgatgggcc atgtcagcta tgccggtcat ctggcacagg
     4321 ccggtgtgga tatcacgctg atttacgccg gatcgcacaa ggtggatggc aatcagtttg
     4381 aatccctgcc agcaaaggtt cgccaggaca tgcagcagcg cattgatgcg gcgcgccgga
     4441 tgtttgctga aaaagtggcg atgtataccg gtttatctgt ggatgcggtt atgggaacag
     4501 aggccgccgt ttttgaaggt cagtccggta ttgaggccga actggcggat gaattaatca
     4561 atgcgtcgga cgccatcagc gtgatggctg cgacgctgaa cacacacgat acaggaggca
     4621 ctatgccgca attaactgca acggaggctg ccgcgcagga gaaccagcga gtgatgggga
     4681 tcctgacgtg tcaggaagcg aaaggacgtg aacaactggc cacgatgctg gcagggcaac
     4741 agggtatgag cgttgagcag gcccgggcga ttctggccgt ggcggcaccg caacaatctg
     4801 gtgtatctgc gcagagtgaa gccgatcgca ttatggcatg tgaagaggcg aaaggacgtg
     4861 aacaactggc ggcaacgctg gcggcgatgc cggagatgac ggtggaaaaa gcccgtccga
     4921 ttctggctgc ttcaccgcag gcgaatgccg gaccctcact tcgtgatcag attatggccc
     4981 tggatgaggc aaaaggggca gaggcgcagg ctgaaaaact ggcggcctgt ccggggatga
     5041 ccgtggagaa tgcccgggct gtactggcgg cagcctcaga cagggcagaa ccggtttctg
     5101 catccacaac cgccatgttt gaacatttca tggcgaacca ttcaccggca gcggtacagg
     5161 gtggcgtgcc acagacgtca gcagacggtg atgcggacgt gaaaatgctc atggccatgc
     5221 catgaagtca gtgctgacca tcaatatgag gtttttacaa tatggtaacg aaaaccatca
     5281 ctgaacagcg tgcggaagta cgtatttttg ccggtaatga tccggcccat accgccacag
     5341 gcagcagcgg gatttcctcg gcaacaccgg ctctgacgcc cctgatgctg gatgaggata
     5401 ccgggaaact ggtggtctgg gacggacaga aggcaggcca tgtggttggc atactggtac
     5461 tgccgcttga aggtacagag acggtgctga cgtattacaa gtcggggacc tttgcgacgg
     5521 aggccatcca ctggcctgag agagtggata cacacaaaaa ggcgaatgcc tttgccggca
     5581 gtgccctgag tcacgcagcg ctgccgtaac attttatcag gtcactgcgg tagcctgact
     5641 gatttctgaa tgaaaggaac tgatttatgg gattgtttac gacccgccag ttactcggtt
     5701 ataccgaaca aaaagttaaa tttcgtgcgc tgtttctgga gctgtttttc cgccgtacgg
     5761 tgaatttcca taccgaagag gtaatgctgg acaaaattac cggaaaaacg ccggtggcgg
     5821 cctatgtctc cccggttgtt gaaggaaagg tgttgcgtca ccgtggcggt gaaactcgtg
     5881 tactgcgccc gggctacgtt aagccgaaac acgaatttaa ttaccagcag gcggtggagc
     5941 gtcttcccgg tgaagatccg gcccagctga acgacccggc ttaccgtcgc ctgcgtatca
     6001 tcaccgataa cctcaaacag gaagagcacg ccattgtcca ggtggaagag atgcaggcgg
     6061 tgaatgccgt gctgtatggc aaatacacca tggaaggaga gcagtttgaa actgttgagg
     6121 tcgattttgg ccggtcggtg ggaaataaca ttacgcaggc aggagggacg gagtggagcg
     6181 ctcaggaccg cgataccttt gatccgacgc atgatctgga tgcgtactgt gattttgctt
     6241 ccggcaccat caatattgcc attatggatg gtaaggtctg gcgtctgctg aatggcttta
     6301 agctgttccg cgaaaaactg gatacccgtc gtggctcaaa ttcacagctg gaaacggcag
     6361 tgaaagacct gggggcggtg gtgtccttca aagggtatta tggtgatctg gcaatcgtgg
     6421 tggcaaaaac gtcctatgtg gcagaggacg gtaccgaaaa acgttatctg ccggacggca
     6481 cactggtact gggaaatacg gccgcagagg gtattcgttg ctacggtgcc attcaggatg
     6541 cgcaggcatt gtctgaaggc gtggtggcgt cttcccgtta tccgaaacac tggctgaccg
     6601 tgggtgatcc ggcgcgtgaa ttcaccatga cgcagtccgc gccgctgatg gtgctgccgg
     6661 atccggatga gtttgtggtg gtgcaggtga aataatccgt gagcgggagc taaatgctcc
     6721 cgtttttcat tcaatcagga gcgggtatgg ctacgaaaga agaaaacctg aaacgtcttt
     6781 gtgagctggc tgagaggctg gggcgtgaac cggatgtctc cggtagtgcg gcagatattg
     6841 cgcagcgagt ggcggaactg gaggaagagc ttggagatgc gggggaggtc gcagagccgg
     6901 atacgtactc accatcgaaa gatgatccga ccgcgcataa aggcgagtca gttcctgaac
     6961 agccaggcat tgttaccgga gacgaaacgg ggcaggttac ggtggtggca ctggcgacac
     7021 tgcatacaaa atgtaatggt tcagtgatat ttgtttcacc gggaacgtcg tttcgtgttt
     7081 ctgctgaggt ggctgccagc atggcggcag gtggtctggc aaaaaggcag taacaggaga
     7141 tgtcgtggac ggtttcgata atctgtttga tgctgcgctt gccggtgtca atgaagtcat
     7201 tctccgtgat atggggatca gcgccgtcat tacgtccgga gaacttgagg ggacacacct
     7261 gacaggggtt tttgatgatc ccgaaagtat ttcttttgtt gccgggggga tccggcttga
     7321 ggattcctca ccgtgcctgt ttgtgaaaac ggcagatata tcgcagttac gccgtcagga
     7381 tacgttaact atcggggatg attctttctt tgtggatcgt attactccgg atgacggggg
     7441 atgctgttat atccggttaa ggcggggcag tcctgcccca cagaataatg ccagaatgag
     7501 gcgatatgat gaagggactt gagaatgcca tccgcaatct gaacagcctc gatacccgca
     7561 tggtgccaca gaccagcgta tgggcggtaa accgtgtggc ggcaaagatt gtttctgtcg
     7621 ccacacggca ggttgcgcag aataccgttg ccggggataa ccaggtgaaa ggtattcccc
     7681 tgaaaacggt cagggagcgt gtcaggctgc ttaaagccag cccctcagga aaaatgtatg
     7741 cccgcatgcg tgttaaccgg ggtaacctgc ccgccattaa gctgggtacc gcacaggtca
     7801 gactggcccg ctcccggcat ggcagcaact cacgtcatcg tggcagcgta ctgaaagtgg
     7861 ggaaatacct tttccgggat gcgtttattc agcaactggc gaatggtcgc tggcatgtga
     7921 tgcggcgtat tgacggcaaa aatcgttacc ccattgatgt ggtgaaaatc ccg
//
LOCUS       DAJVPP010000152         7910 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVPP010000152 DAJVPP010000000
VERSION     DAJVPP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.152"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(123..953)
                     /locus_tag="OQM34_003705"
     CDS             complement(123..953)
                     /locus_tag="OQM34_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCS1453970.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OQM34_003706"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OQM34_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1453971.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2145..2543
                     /locus_tag="OQM34_003707"
     CDS             2145..2543
                     /locus_tag="OQM34_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016808810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1453972.1"
                     /translation="MRCRPSLVRGRAAQVNPGGFTTPARRPSVPHRTAGCGKSSLRPL
                     MAGSSPSLPDGSNPSAAIVQSASDVQCSRLLKTTMEVVAEGVETPDCLAWLRQAGCDT
                     VQGFLFARPMPAAAFVGFVNQWRNTTMNAN"
     gene            2562..2729
                     /locus_tag="OQM34_003708"
     CDS             2562..2729
                     /locus_tag="OQM34_003708"
                     /inference="COORDINATES: protein motif:HMM:NF023237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCS1453973.1"
                     /translation="MCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATETSV
                     KPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OQM34_003709"
     CDS             2855..3415
                     /locus_tag="OQM34_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1453974.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OQM34_003710"
     CDS             3418..6384
                     /locus_tag="OQM34_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCS1453975.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OQM34_003711"
     CDS             6451..6828
                     /locus_tag="OQM34_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1453976.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OQM34_003712"
     CDS             complement(7029..7688)
                     /locus_tag="OQM34_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCS1453977.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1752 a         2242 c         2133 g         1783 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat taaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaggg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
//
LOCUS       DAJVPP010000153         7608 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVPP010000153 DAJVPP010000000
VERSION     DAJVPP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.153"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..584)
                     /gene="msyB"
                     /locus_tag="OQM34_003713"
     CDS             complement(<1..584)
                     /gene="msyB"
                     /locus_tag="OQM34_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1453978.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAG"
     gene            complement(610..1014)
                     /locus_tag="OQM34_003714"
     CDS             complement(610..1014)
                     /locus_tag="OQM34_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS1453979.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            1391..3307
                     /gene="dnaK"
                     /locus_tag="OQM34_003715"
     CDS             1391..3307
                     /gene="dnaK"
                     /locus_tag="OQM34_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1453980.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3396..4526
                     /gene="dnaJ"
                     /locus_tag="OQM34_003716"
     CDS             3396..4526
                     /gene="dnaJ"
                     /locus_tag="OQM34_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1453981.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4630..4782)
                     /gene="mokC"
                     /locus_tag="OQM34_003717"
     CDS             complement(4630..4782)
                     /gene="mokC"
                     /locus_tag="OQM34_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1453982.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            5368..6534
                     /gene="nhaA"
                     /locus_tag="OQM34_003718"
     CDS             5368..6534
                     /gene="nhaA"
                     /locus_tag="OQM34_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1453983.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            6600..7499
                     /gene="nhaR"
                     /locus_tag="OQM34_003719"
     CDS             6600..7499
                     /gene="nhaR"
                     /locus_tag="OQM34_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1453984.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVTDA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
BASE COUNT         1900 a         1801 c         1967 g         1940 t
ORIGIN      
        1 cccgcgccgc gcagcaaacc atgcccaagt acgctcattg ctgcgtgggt gcgtaaaatg
       61 cgggtcagtt ggctggaaag caaatgcgac acaccttttg ccaataattt gtctttcatc
      121 agcagcggca gcagctcttc cagctcattc accctggcat cgaccgcgtg cagaaactcc
      181 tgcttatgtt cctcgtccat tttcttccag gtattacgca gaaattgttc cagtaactgt
      241 tgctcaattt caaacgtaga catctctttg tcggctttca gcttcaatcg ctttgaaaca
      301 tcgagcaaaa tggcccgata caatttaccg tgtccgcgca gtttgttggc gatactatcg
      361 ccaccaaaat gctgtaattc tccggcaatc agctgccagt tgcggcgatg ttgctcggga
      421 tgcccttcca tcgatttaaa cagttcgttg cgcatcagta tgctggagag gcgagttttg
      481 cctttttcat tatgggtgag caatcgggcg aaatttgcca actgttcctc actacaatgc
      541 tgaagaaaat ccagatctga atcattcagg taattaacat tcattttttg tggcttctat
      601 attctggcgt tagtcgtcgc cgataatttt cagcgtggcc atatccgatg agttcaccgt
      661 atgaccggaa aaggtgattt ttgagacgca gcgtttattg tcgttatcgc tgttaatgtt
      721 gatccagtca gtggtttgcc cttcttttat ttctgaagga atattcaggc tctgactggc
      781 gctacgggcg gctttgaaat aaaccgatgc accacttaac tgtaaatcgc catggtcggc
      841 agagagttgt atgcgtttca caatgcgaca aacaggaagt ttcagcgcca gatcgttggt
      901 ttcgttacgc ggcattgcaa tgacgccgag gagtttatgg tcgtttgcct gcgccgtgca
      961 gcacagcatc aggctaatcg ccaggctggc ggaaatcgta aaaacggatt tcataaggat
     1021 tctcttagtg ggaagaggta gggggatgaa tacccactag tttactgctg ataaagagaa
     1081 gattcaggca cgtaatcttt tctttttatt acaatttttt gatgaacgcc ttggctgcga
     1141 ttcattcttt atatgaataa aattgctgtc aattttacgt cttgtcctgc catatcgcga
     1201 aatttctgcg caaaagcaca aaaaattttt gcatctcccc cttgatgacg tgggttacga
     1261 ccccatttag tagtcaaccg cagtgagtga gtctgcaaaa aaatgaaatt gggcagttga
     1321 aaccagacgt ttcgccccta ttacagactc acaaccacat gatgaccgaa tatatagtgg
     1381 agacgtttag atgggtaaaa taattggtat cgacctgggt actaccaact cttgtgtagc
     1441 gattatggat ggcaccattc ctcgcgtgct ggagaacgcc gaaggcgatc gcaccacgcc
     1501 ttctatcatt gcctataccc aggatggtga aactctggtt ggtcagccgg ctaaacgtca
     1561 ggcagtgacg aacccgcaaa acaccctgtt tgcgattaaa cgcctgattg gccgccgctt
     1621 ccaggacgaa gaagtacagc gtgatgtttc catcatgccg ttcaaaatta ttgctgctga
     1681 taacggcgac gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc agatttctgc
     1741 tgaagtgctg aaaaaaatga agaaaaccgc tgaagattac ctgggtgaac cggtaactga
     1801 agctgttatc accgtaccgg catactttaa cgatgctcag cgtcaggcaa ccaaagacgc
     1861 aggccgtatc gctggtctgg aagtaaaacg tatcatcaac gaaccgaccg cagctgcgct
     1921 ggcttacggt ctggacaaag gcactggcaa ccgtactatc gcggtttatg acctgggggg
     1981 tggtactttc gatatttcta ttatcgaaat cgacgaagtt gacggcgaaa aaaccttcga
     2041 agttctggca accaacggtg atacccacct gggtggtgaa gacttcgaca gccgtctgat
     2101 caactatctg gttgaagaat tcaagaaaga tcagggcatt gacctgcgca acgatccgct
     2161 ggcaatgcag cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt cttccgctca
     2221 gcagaccgac gttaacctgc catacatcac tgcagacgcg accggtccga aacacatgaa
     2281 catcaaagtg actcgtgcga aactggaaag cctggttgaa gatctggtaa accgttccat
     2341 tgagccgctg aaagttgcac tgcaggatgc tggcctgtcc gtatctgata tcgacgacgt
     2401 tatcctcgtt ggtggtcaga ctcgtatgcc aatggttcag aagaaagttg ctgagttctt
     2461 tggtaaagag ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg gtgctgctgt
     2521 tcagggtggt gttctgactg gcgacgtaaa agacgtactg ctgctggacg ttaccccgct
     2581 gtctctgggt atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga aaaacaccac
     2641 tatcccgacc aagcacagcc aggtgttctc taccgctgaa gacaaccagt ctgcggtaac
     2701 catccatgtg ctgcagggtg aacgtaaacg tgcggctgat aacaaatctc tgggtcagtt
     2761 caacctagat ggtatcaacc cggcaccgcg cggcatgccg cagatcgaag ttaccttcga
     2821 tatcgatgct gacggtatcc tgcacgtttc cgcgaaagat aaaaacagcg gtaaagagca
     2881 gaagatcacc atcaaggctt cttctggtct gaacgaagat gaaatccaga aaatggtacg
     2941 cgacgcagaa gctaacgccg aagctgaccg taagtttgaa gagctggtac agactcgcaa
     3001 ccagggcgac catctgctgc acagcacccg taagcaggtt gaagaagcag gcgacaaact
     3061 gccggctgac gacaaaactg ctatcgagtc tgcgctgact gcactggaaa ctgctctgaa
     3121 aggtgaagac aaagccgcta tcgaagcgaa aatgcaggaa ctggcacagg tttcccagaa
     3181 actgatggaa atcgcccagc agcaacatgc ccagcagcag actgccggtg ctgatgcttc
     3241 tgcaaacaac gcgaaagatg acgatgttgt cgacgctgaa tttgaagaag tcaaagacaa
     3301 aaaataatcg ccctataaac gggtaattat actgacacgg gcgaagggga atttcctctc
     3361 cgcccgtgca ttcatctagg ggcaatttaa aaaagatggc taagcaagat tattacgaga
     3421 ttttaggcgt ttccaaaaca gcggaagagc gtgaaatcag aaaggcctac aaacgcctgg
     3481 ccatgaaata ccacccggac cgtaaccagg gtgacaaaga ggccgaggcg aaatttaaag
     3541 agatcaagga agcttatgaa gttctgaccg actcgcaaaa acgtgcggca tacgatcagt
     3601 atggtcatgc tgcgtttgag caaggaggca tgggcggcgg tggttttggc ggcggcgcag
     3661 acttcagcga tatttttggt gacgttttcg gcgatatttt tggcggcgga cgtggtcgtc
     3721 aacgtgcggc gcgcggtgct gatttacgct ataacatgga gctcaccctc gaagaagctg
     3781 tacgtggcgt gaccaaagag atccgcattc cgactctgga agagtgtgac gtttgccacg
     3841 gtagcggtgc aaaaccaggt acacagccgc agacctgtcc gacctgtcat ggttctggtc
     3901 aggtgcagat gcgccaggga ttcttcgctg tacagcagac ctgtccacac tgtcagggcc
     3961 gcggtacgct gatcaaagat ccgtgcaaca aatgtcatgg tcatggtcgt gttgagcgca
     4021 gcaaaacgct gtccgttaaa atcccggcag gggtggacac tggagaccgc atccgtcttg
     4081 cgggcgaagg tgaagcgggc gagcatggcg caccggcagg cgatctgtac gttcaggttc
     4141 aggttaaaca gcacccgatt ttcgagcgtg aaggcaacaa cctgtattgc gaagtcccga
     4201 tcaacttcgc tatggcggcg ctgggtggcg aaatcgaagt accgaccctt gatggtcgcg
     4261 tcaaactgaa agtgcctggc gaaacccaga ccggtaagct attccgtatg cgcggtaaag
     4321 gcgtcaagtc tgtccgcggt ggcgcacagg gtgatttgct gtgccgcgtt gtcgtcgaaa
     4381 caccggtagg cctgaacgaa aagcagaaac agctgctgca agagctgcaa gaaagcttcg
     4441 gtggcccaac cggcgagcac aacagcccgc gctcaaagag cttctttgat ggtgtgaaga
     4501 agttttttga cgacctgacc cgctaacctc cccaaaagcc tgcccgtggg caggcctggg
     4561 taaaaatagg gtgcgttgaa gatatgcgag cacctgtaaa gtggcgggga tcactccccg
     4621 ccgttgctct tactcggatt cgtaagccgt gaaaacagca acctccgtct ggccagttcg
     4681 gatgtgaacc tcacagaggt cttttctcgt taccagcgcc gccactacgg cggtgataca
     4741 gatgacgatc agggcgacaa tcatcgcctt atgctgcttc attgctctct tctccttgac
     4801 cttacggtca gtaagaggca ctctacatgt gttcagcata taggaggcct cgggttgatg
     4861 gtaaaatatc actcggggct tttctctatc tgccgttcag ctaatgcctg agacagacag
     4921 cctcaagcac ccgccgctat tatatcgctc tctttaaccc attttgtttt atcgattcta
     4981 atcctgaaga cgcctcgcat ttttgtggcg taatttttta atgatttaat tatttaactt
     5041 taatttatct cttcatcgca attattgacg acaagctgga ttatttttga aatattggcc
     5101 taacaagcat cgccgactga caacaaatta attattactt ttcctaatta atccctcagg
     5161 aatcctcacc ttaagctatg attatctggg cttagggtca ctcgtgagcg cttacagccg
     5221 tcaaaaacgc atctcaccgc tgatggcgca aattcttcaa tggctcgtaa aaaacgaatt
     5281 attcctacac tataatctga ttttaacgat gattcgtgcg gggtaaaata gtaaaaacga
     5341 tctattcacc tgaaagagaa ataaaaagtg aaacatctgc atcgattctt tagcagtgat
     5401 gcctcgggag gcattattct tatcattgcc gctatcctgg cgatgattat ggccaacagc
     5461 ggcgcaacca gtggatggta tcacgacttt ctggagacgc cggttcagct ccgggttggt
     5521 tcactcgaaa tcaacaaaaa catgctgtta tggataaatg acgcgctgat ggcggtattt
     5581 ttcctgttag tcggtctgga agttaaacgt gaactgatgc aaggatcgct agccagctta
     5641 cgccaggccg catttccagt tatcgccgct attggtggga tgattgtgcc ggcattactc
     5701 tatctggctt ttaactatgc cgatccgatt acccgcgaag ggtgggcgat cccggcggct
     5761 actgacattg cttttgcact tggtgtactg gcgctgttgg gaagtcgtgt tccgttagtg
     5821 ctgaagatct ttttgatggc tctggctatt atcgacgatc ttggggccat cattatcatc
     5881 gcattgttct acactaatga cttatcgatg gcctctcttg gcgtcgcggc tgtagcaatt
     5941 gcggtactcg cggtattgaa tctgtgtggt gtacgccgca cgggcgtcta tattcttgtt
     6001 ggcgtggtgt tgtggactgc ggtgttgaaa tcgggggttc acgcaactct ggcgggggta
     6061 attgtcggct tctttattcc tttgaaagag aagcatgggc gttctccagc gaagcgactg
     6121 gagcatgtgt tgcacccgtg ggtggcgtat ctgattttgc cgctgtttgc atttgctaat
     6181 gctggcgttt cactgcaagg cgtcacgctg gatggcttga cctccattct gccattgggg
     6241 atcatcgctg gcttgctgat tggcaaaccg ctggggatta gtctgttctg ctggttggcg
     6301 ctgcgtttga aactggcgca tctgcctgag ggaacgactt atcagcaaat tatggtggtg
     6361 gggatcctgt gcggtatcgg ttttactatg tctatcttta ttgccagcct ggcctttggt
     6421 agcgtagatc cagaactgat taactgggcg aaactcggta tcctggtcgg ttctatctct
     6481 tcggcggtaa ttggatacag ctggttacgc gttcgtttgc gtccatcagt ttgacaggac
     6541 ggtttaccgg ggagccataa acggctccct tttcattgtt atcagggaga gaaatgagca
     6601 tgtctcatat caattacaac cacttgtatt acttctggca tgtctataaa gaaggttccg
     6661 tggttggcgc agcggaggcg ctttatttaa ctccacaaac cattaccgga cagattcgag
     6721 cgctggaaga gcgcctgcaa ggcaaattat ttaaacgcaa gggacgtggt ctcgaaccca
     6781 gcgagctggg agaactggtc tatcgctatg ccgataaaat gttcacctta agccaggaaa
     6841 tgctggatat tgtgaactat cgcaaagaat ccaatttatt gtttgacgtt ggcgtgactg
     6901 atgcactttc caaacgcctg gtcagtagcg tacttaacgc cgcagtggta gaaggcgagc
     6961 ccattcatct tcgctgcttc gaatccaccc acgaaatgct gctggagcaa ttaagtcagc
     7021 ataaactgga tatgatcatt tctgactgtc cgatagactc tacgcagcag gaaggcctgt
     7081 tctccgtgag aattggcgaa tgtggcgtga gtttctggtg tacaaatcca ccaccagaaa
     7141 aaccgttccc ggcttgtctg gaagaacggc gacttttgat tcctgggcga cgttcaatgt
     7201 tagggcgcaa attgcttaac tggtttaact cccagggatt aaacgtagaa atcctcggcg
     7261 agtttgatga tgccgctttg atgaaagctt ttggtgcgat gcacaatgca atcttcgttg
     7321 ccccaacgct ttatgcatat gacttttatg ccgataaaac tgtcgtagaa attggtcgcg
     7381 tcgagaatgt gatggaagag taccatgcta tttttgctga gcggatgatt cagcacccgg
     7441 cggtacagcg aatctgcaat acggattatt ctgcgctttt tagtccagcg gtgcgttaat
     7501 cggcagctcc cccaaagtta aggtggggga gatagattag ttgtacatta ccacgatttt
     7561 gactcggctc attatttgcc cgcttgagac attgtttcca tatgtacg
//
LOCUS       DAJVPP010000154         7535 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVPP010000154 DAJVPP010000000
VERSION     DAJVPP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.154"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..1159
                     /gene="rbsA"
                     /locus_tag="OQM34_003720"
                     /pseudo
     CDS             1..1159
                     /gene="rbsA"
                     /locus_tag="OQM34_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1164..2129
                     /gene="rbsC"
                     /locus_tag="OQM34_003721"
     CDS             1164..2129
                     /gene="rbsC"
                     /locus_tag="OQM34_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1453985.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2154..3044
                     /gene="rbsB"
                     /locus_tag="OQM34_003722"
                     /pseudo
     CDS             2154..3044
                     /gene="rbsB"
                     /locus_tag="OQM34_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3170..4099
                     /gene="rbsK"
                     /locus_tag="OQM34_003723"
     CDS             3170..4099
                     /gene="rbsK"
                     /locus_tag="OQM34_003723"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1453986.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANISFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4103..5095
                     /gene="rbsR"
                     /locus_tag="OQM34_003724"
     CDS             4103..5095
                     /gene="rbsR"
                     /locus_tag="OQM34_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1453987.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OQM34_003725"
     CDS             complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OQM34_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1453988.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6511..7203)
                     /locus_tag="OQM34_003726"
     CDS             complement(6511..7203)
                     /locus_tag="OQM34_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1453989.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1920 a         1824 c         1983 g         1808 t
ORIGIN      
        1 aaaccatgta tgtcgaagcg gataaattgc tggctaaact taacctgcgc tttaaaagcg
       61 acaagttagt gggcgatctt tccatcggtg accagcaaat ggttgaaatc gccaaagtgc
      121 tgagctttga gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg accgataccg
      181 aaaccgaatc cctgttccgc gtcatccgcg agctgaaatc gcaaggccac tgtattgtct
      241 atatctccca ccgcatgaaa gaaatcttcg agatttgcga tgacgttacc gtttttcgtg
      301 atgggcaatt tattgctgag cgcgaagtgg catcactgac cgaagattcg ctgattgaga
      361 tgatggtggg ccgcaagctg gaagatcaat atccacacct ggacaaagcg ccaggagata
      421 tccgcctgaa agtcgataat ctctgcggac ctggcgttaa cgatgtctct tttactttac
      481 gcaaaggcga aattcttggc gtctctggtt tgatggacgc aggtcgtacc gaactgatga
      541 aagtgctcta cggcgcactg ccgcgcacca gcggttacgt caccctggat gggcatgaag
      601 tcgttacccg ttcaccgcag gatggcctgg caaacggcat tgtgtatatc tccgaagacc
      661 gtaaacgtga cggtttagtg ctgggcatgt cagtaaaaga gaacatgtcg ctgacagcgc
      721 tgcgctactt cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag caggctgtga
      781 gtgatttcat tcgtctgttt aatgtgaaaa cgccgtcgat ggaacaggca attggtctgc
      841 tttccggtgg caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca cccccaaagt
      901 attgatcctt gatgagccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
      961 actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat cggagatgcc
     1021 agaagtatta ggcatgagca atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
     1081 atttactcgt gagcaggcca cccaggaagt gttaatggct gtcgctgtgg gcaagcttaa
     1141 tcgcgtgaat caggagtaaa aaaatgataa cccagactgt ctctggtcgc cgttatttca
     1201 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
     1261 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
     1321 cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatag
     1381 acttgtcggt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca
     1441 ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc gcaattggtg
     1501 cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta
     1561 tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca gtgaataccg
     1621 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac
     1681 cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca
     1741 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg
     1801 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
     1861 tggccgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg gggactggct
     1921 atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
     1981 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
     2041 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
     2101 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
     2161 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
     2221 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
     2281 cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg gtggtgctgg
     2341 actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
     2401 ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct gtgaagatgg
     2461 ctaactaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg
     2521 tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat tacatcgcga
     2581 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt acatccgcag
     2641 cccgtgaacg tggcgaaggc ttccagctgg ccgttgctgc tcacaagttt aatgttcttg
     2701 ccagccagcc agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga
     2761 ccgctcatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc
     2821 tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac
     2881 cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg
     2941 atcagattgg cgcgaaaggc gttgaaaccg cagataaagt gctgaaaggc gagaaagttc
     3001 aggctaagta tctggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta
     3061 tgacctgatg gtgacataaa tacgtcatcg acagatgaac gtgtaatata aagaaaagca
     3121 gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc
     3181 aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt
     3241 tcctactcca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg
     3301 cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat atctcgttta ttgcctgtac
     3361 tggcgatgac agcattggcg agagcgttcg ccagcagctc gccactgata acatcgatat
     3421 tactccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg
     3481 cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgctcttt ccccatcgct
     3541 ggtggaagcg caacgtgagc gtattgccaa cgcgtcagcg ttattaatgc agctggaatc
     3601 accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc
     3661 gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcgctgg tggacattat
     3721 tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga
     3781 tgcagcgaag gcggcgcagg tactgcatga aaagggtatc cgtactgtac tgattacttt
     3841 aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg
     3901 ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggcgcgt taatcacggc
     3961 gttgctggaa gaaaaaccat tgccagaggc gattcggttt gcccatgctg ccgctgcgat
     4021 tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt
     4081 tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgtt tggcgggcgt
     4141 ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac
     4201 cgccaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagctc tggcgcgtag
     4261 cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt
     4321 ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt
     4381 cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca
     4441 aaaacgcgtt gatggcttac tgttactgtg taccgaaacg catcagcctt cgcgtgaaat
     4501 catgcaacgt tatccgacag tgcctaccgt gatgatggac tgggctccgt tcgatggcga
     4561 cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat
     4621 cgataaaggt catacccgta tcgcctgtat taccggtccg ctggataaaa ctccggcgcg
     4681 cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg
     4741 ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgccatgc gccaactgtt
     4801 atcacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt
     4861 ttaccaggcg ttatatcagg cagagttaca ggttccgcag gatatcgcgg tgattggcta
     4921 tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc accaaccgaa
     4981 agatgaactg ggggagctgg cgattgatgt actcatccat cggataaccc agccgaccct
     5041 tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg catagattta
     5101 cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct
     5161 gaagcaacag taataatgcc catcgtgata aacgtatagt ggaattgttc gacagtcgtt
     5221 gtgccttcca tcccttcata aatgcgaagg acggccgcac ttacagcaac gcctaaacta
     5281 atcgacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc
     5341 agatcggcaa gtgtgatggt attcatcgcg gtaaactgcg tcgacatagc catccctaat
     5401 ataaacaacg gcaagatcag catccatatc gccattgccg gtgattgcaa agagaactga
     5461 gcgatcatta gcccaataat caccgtgatc cccactaatg tatggcgata gcccagacga
     5521 cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc
     5581 atacagccgg caataaacgc ctgataacca aatcctacct gtaacatcaa tggcataagg
     5641 aacggtacac agccggtccc cagacgggtt gcaatattgc ctacgatacc gatcgagaaa
     5701 gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtacgtcg cgcatggaga
     5761 atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccagctggcg
     5821 acaatctttt ccccgaatag ctctattccg cttgaaaaga gaacaaggct gaggccaaac
     5881 agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg
     5941 cgcgcgtaaa gaaggcccgc aatacctatg gggatattaa ttaaaaatat ccagtgccag
     6001 gttgcccagg tgaccagcac gccgccaaga acggggccta aaattggccc caccagaccc
     6061 ggcatggcga caaaattcaa taccggaaga agttcattac gaggataagc acgcagtaag
     6121 gccggccgag caacaggcat catcattgcg tcgcctatcc cttgaataac ccggaagaca
     6181 accagctgtg gtagcgaatt agaaagtgcg caggccagag aacccaacgt gaacagactc
     6241 acggcaaggg taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga
     6301 ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa
     6361 cgattaaggc tatgagcgat tgcgggtaag gcggtattca gaatagtggc atcaagtgcc
     6421 tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg acatactacg cttcttttta
     6481 tcgctcattc aatgtcctgt tatcgggtta tcacttatca ggtgagcgta gcagcgcctg
     6541 acaagcttta aatgccgcgt cgccatcgct ttggataatc gcatcgacaa tcgcctggtg
     6601 cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga
     6661 atggaatagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc
     6721 atgccaggcc atatcgactt cgatccagcg ttcacggcga aagttctctt ttaatgccgc
     6781 catttcggcc attaacgtat tgagatgcgc cttctgttcc gcggtgccaa ccgttgctgc
     6841 cagtaggcag gcttgcggct ccagacagat acgcataacc agaaagtgat cgatgacctg
     6901 atgaaagttc tcttctgtta tccaccaagt aagcaattcc tgatcaagaa aattccagtt
     6961 cgattgtggc atgacccgag taccaattcg cggtcgcggt aaaaccatcc cttttgccgt
     7021 taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc
     7081 aatctcaccg ggcaaaatgg tgccgggttc atattcacct tttaagatcc gttgcgccag
     7141 cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg
     7201 cattacttat cttccttttt ctttttattc ctccttagta tgccaccagg aagtgtgatt
     7261 acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacggggta ttttgctgaa
     7321 aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta
     7381 taatacgcca ccactgacac ggaacaacgg caaatacgcc gccgggtcag cgggggtctc
     7441 ctgagaattc cgacagagaa agcaaaaata aatgattgac tctgtagcgg gaaagcgtat
     7501 tatgcacacc cccgcgccgc tgagaaaaag cgaag
//
LOCUS       DAJVPP010000155         7475 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVPP010000155 DAJVPP010000000
VERSION     DAJVPP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.155"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..941
                     /locus_tag="OQM34_003727"
     CDS             <1..941
                     /locus_tag="OQM34_003727"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1453990.1"
                     /translation="LATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPFQK
                     WQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPHFI
                     ALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMIDSI
                     KDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKDYL
                     LYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEALHR
                     QVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1007..1255
                     /locus_tag="OQM34_003728"
     CDS             1007..1255
                     /locus_tag="OQM34_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCS1453991.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1320..1688
                     /locus_tag="OQM34_003729"
     CDS             1320..1688
                     /locus_tag="OQM34_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1453992.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            1782..2435
                     /gene="nfsB"
                     /locus_tag="OQM34_003730"
     CDS             1782..2435
                     /gene="nfsB"
                     /locus_tag="OQM34_003730"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS1453993.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            2543..3790
                     /gene="ybdG"
                     /locus_tag="OQM34_003731"
     CDS             2543..3790
                     /gene="ybdG"
                     /locus_tag="OQM34_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1453994.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(3858..5234)
                     /gene="pheP"
                     /locus_tag="OQM34_003732"
     CDS             complement(3858..5234)
                     /gene="pheP"
                     /locus_tag="OQM34_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1453995.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5335..>7475)
                     /gene="cusA"
                     /locus_tag="OQM34_003733"
     CDS             complement(5335..>7475)
                     /gene="cusA"
                     /locus_tag="OQM34_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCS1453996.1"
                     /translation="DNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFI
                     VMPFQGLNANIMSLGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQ
                     VITDASVEVGPALFISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIV
                     VIPILMGYWIRGKIPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWP
                     LNKVGGEFLPQINEGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGK
                     AETATDSAPLEMVETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNR
                     IDMLSTGIKSPIGIKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVE
                     INREKAARYGMTVADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQ
                     LPILTPMKQQITLADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKA
                     IAEKVQLKPGTSVAFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLII
                     SSVPFALVGGIWLLWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLN
                     NPQTFSEQKLDEALYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMI
                     GGMITAPLLSLFIIPAAYKLMWLHRHRVRN"
BASE COUNT         1840 a         2043 c         1834 g         1758 t
ORIGIN      
        1 agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcagttt tcagcgatgc
       61 agaaagtcgt attgcaggca gccgcagacc atcatctgga aatttgcggc ggtggcacgc
      121 ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa cgaacgctat caacgaacgc
      181 tggaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat gtccatgttg
      241 gctgtgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg tttgtgccgc
      301 actttattgc cctttccgcc gcgtcgccat atatgcaggg aacggatacg cgttttgccc
      361 cctcacgacc gaatattttt tccgcctttc ctgataatgg cccgatgccg tgggtcagta
      421 actggcaaca atttgaagcc ctgtttcgct gtctgagtta caccacgatg atcgacagca
      481 ttaaagatct gcactgggat attcgcccca gtcctcattt tggcacggtg gaggttcggg
      541 tgatggatac cccgttaacc cttagccacg cggtaaatat ggcgggatta attcaggcca
      601 ccgcccactg gttactgacg gaacgcccgt tcaaacatca ggagaaagat tacctgctgt
      661 ataaattcaa ccgtttccag gcctgtcgct atggtctgga aggcgtcatt accgatccgc
      721 acactggcga tcgtcgaccg ctaacggaag ataccttgcg attgctggaa aaaatcgccc
      781 cttcagcaca taaaattggc gcatcgagcg caattgaagc actgcatcgc caggtcgtca
      841 gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc cgatggcggc tcgctgattg
      901 ggctggtgaa aaagcattgt gagatctggg ccggtgacta agccgggaat tgccttacag
      961 aagaatatcc ctgacaatgg tgttttaact tacatttaac aatgttatga aacacccttt
     1021 agaaaccttg accaccgcag caggcatttt gctgatggct ttcctctctt gcctgctgct
     1081 gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccatgt tccatctgat
     1141 ggatcttagc cagctttaca ctttattgtt ttgtctgtgg tttttagtgc tgggcgctat
     1201 tgagtatttt gttctgcgct ttatctggcg acgctggttc tcgctggcgg attaaacgtc
     1261 gattatcgcc gaacttcgcg gcatactttg cttattctcc ttcgcctaaa gaaacgttta
     1321 tggataagca atcactgcac gaaacggcga aacgcctagc tcttgagtta ccctttgtcg
     1381 agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc aagattttta
     1441 tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct gaagtccgat ccacaaaaat
     1501 ccctgttaaa tcagcaaata tacccaagca ttaagccagg gtatcacatg aataaaaagc
     1561 actggatttc ggtgtattcc ggcgaggaaa tctccgaagc gttacttcgc gatctgatca
     1621 acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa agagtgcgtc
     1681 caggctaaag cgggaatcta tagcgcattt ttctcgctta ccatttctcg ttgaaccttg
     1741 taatctgctg gcacgcaaaa ttacttacac atggagtctt tatggatatc atttctgtcg
     1801 ccttaaagcg tcattccact aaggcatttg atgccagcaa aaaacttacc ccggaacagg
     1861 ccgagcagat caaaacgcta ctgcaataca gcccatccag caccaactcc cagccgtggc
     1921 attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc gctgccggta
     1981 attatgtgtt caacaaacgt aaaatacttg atgcctcgca cgtcgtggtg ttctgtgcaa
     2041 aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat gccgatggcc
     2101 gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc ttcgccgata
     2161 tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt tatctcaacg
     2221 tcggtaactt cctgctcggc gtggcggcgc tgggtctgga cgcggtgccc atcgaaggtt
     2281 ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga gaaaggctac accagtctgg
     2341 tggttgttcc ggtaggtcat cacagcgttg aagattttaa cgctacgctg ccgaaatctc
     2401 gtctgccgca aaacaccacc ttaaccgaag tgtaattctc tcttgccggg catctgcccg
     2461 gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc atcataggct
     2521 gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag atttagcggg
     2581 tattgagatc gatcacacca cctcgatggt gatgattttc ggtattattt ttctgaccgc
     2641 cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa aacgtgccat
     2701 cgccagttca cggctttggt tgcaaatcat tacccagaat aaactcttcc accgtttagc
     2761 ttttaccctg caggggatta tcgtcaatat tcaggcggta ttctggctgc aaaaaggcac
     2821 cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt atgcgctgct
     2881 ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc cggcggcatc
     2941 tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcaa tcctggtcgg
     3001 cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca gcggtcttgg
     3061 tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc tggtggcagg
     3121 tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaaa tgccgaaata
     3181 cggcgcggat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc gtaactggga
     3241 caataccatt accactattc ccacctggtc tctggtttct gactccttta aaaactggag
     3301 cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg atgtcaccag
     3361 tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt tgttaaagcc
     3421 ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag gttctacgga
     3481 gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct atctgaacga
     3541 atatctgcgt aaccatccgc ggattcgtaa agatatgacc ttaatggtac gccaactggc
     3601 tccaggtgat aacggtttac cgctcgagat ctacgcgttt actaacaccg tggtgtggct
     3661 ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg tcgaagagtt
     3721 tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg cgggtgcatt
     3781 taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggacat cgttttgtcg
     3841 gatgtagcgt gaatgcctta tttccgacgc agcgttttaa atgccacaaa caggaataca
     3901 atccacaccg gcagcaggat cgctgacaag cgcatatcat ccatcgtgca catcagcagc
     3961 aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga acggatagag cagcgcctta
     4021 aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa aacgcagatg cgccaggcag
     4081 atcataatcc agttcaacag caacgttgct accaccagcg ccatcagcag accaaacgct
     4141 ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc ggaaagcatc
     4201 agcgagttaa tcggcacacc gcgacggctg acgcgagtca aaaacttcgg cgcattaccc
     4261 tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt atacaccgac
     4321 agcgatgcca ctagaatgac gaagttcagc gcagaagcta ccacattgct gtcgagatta
     4381 tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg atagagtgcc
     4441 agtaaaacca ccagtgaacc gatataaaac agcaggatgc gatacaccac ctgatttacc
     4501 gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg ctgtaatccc aatcagctcc
     4561 agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagcccatt ccagccggtg
     4621 gcgaagaaac caccgtagcg ccagaggttg tcgatactgg ctttctcgcc gccgtaacca
     4681 gaaaacagca gccacaggcc aaagccgatc ataccgatga tcgccagcac tttaatcagc
     4741 gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac ggcgttgatg
     4801 ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca gtactgcata
     4861 tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac ccagtagttc
     4921 cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg ggcaaatgaa
     4981 ccggataccg gctcttcgac caccatctcg ccaagctggc gcataatcag gaaagcgatg
     5041 atcccggcga cgccgtagcc cagcaataca gccggacccg ccatctgaat cgccgggcca
     5101 atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg aatatgacgg
     5161 ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc cgatacggtt
     5221 gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac cttttaacat
     5281 ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cgaggtatcc tgctttaatt
     5341 ccgtacccga tgtcggtgca gccacatcag cttatacgcc gccgggataa taaacagcga
     5401 cagcaaaggt gcggtgatca tgccgccaat catcggtgct gcaatccggc tcatcacctc
     5461 tgaaccagcc cccgttcccc acagaatcgg cagcagaccg gcgataatca ccgccaccgt
     5521 catcgctttc gggcgcacgc gcaggaccgc gccgtgatac aacgcctcat ccagcttctg
     5581 ctcgctgaat gtttgtggat tattcaacga cggctcggcc tctatggcgt gacgtaaata
     5641 catcagcatc accacgccaa attcggcggc gaccccggca agggcaataa agccggtacc
     5701 cgtcgccaca gaaagatgaa agcccatcca ccacagtaac cagatgccgc ccaccagcgc
     5761 aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc cgacggaatg ccagatacaa
     5821 cagcacgaag atgatcatca gtgtcatcgg caccatgagt ttcagcttat ggttggcgcg
     5881 ctccagtagc tcgaactgcc cggagaatgc cacgctggtg cccggtttta actgcacttt
     5941 ttcagctatc gctttttgca aatcgtgaac caccgacacc atgtcacgat cgcgggcatc
     6001 gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc agcatcgacg gtccggtaga
     6061 gactttaacg tcggccacgt ctgccagggt gatttgctgc ttcatcggcg tcaggatcgg
     6121 caactggcgc agtgcctgcg gactatcgcg ccagctttgc ggataacgca gattaattgg
     6181 ataacgggca atcccttcca ccgtttcgcc aaccatcgcc ccgcccaccg cagaagtcac
     6241 aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg gctttttcac ggttaatctc
     6301 aacgttgata tagcgcccac cttccagccg ctcagcaagc gcagaagcta cgcctggcac
     6361 cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg atatccgcca gcacagtgcc
     6421 ggaaacttta atgccgatgg ggcttttaat gccggttgag agcatatcga tacggttacg
     6481 aattggcggc acccacagat tcgccagccc cggcagacgc acggtgttat ccagttcctc
     6541 aatgattttg tccattgtca tgcctggccg ccactgatcc tgtggcttaa gctggatggt
     6601 cgtttctacc atctccagcg gagcagaatc ggtggcggtt tccgctttcc cggttttgcc
     6661 aaatacccgc gccacttcag gtacgctcat aattagcttg tcggtttttt gcagcatact
     6721 cgccgcctct gctgcggaaa tccccggcag cgtcgatggc atatacaaca agtcgccttc
     6781 attgatctgc ggtaaaaatt ccccgccaac tttattgagc ggccagagaa ccgtcagcac
     6841 cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc agtactttca gcaacagcgg
     6901 atgataaaca cgaatcaaaa agcgattgag cggattactg ctttccggcg gaattttgcc
     6961 acggatccag taacccatca ggatagggat cactacgatc gccagcagcg ccgcacccgc
     7021 catcgcatac gttttggtga acgccaacgg gccaaacaga cgcccttcct gcccttccag
     7081 ggtgaagatc gggataaacg acaacgtgat aatcagcaga ctgataaaca gcgccggccc
     7141 cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt ttattatcca gcgtggcgtc
     7201 aggatgctgg tgctgccact cttccagccg tttatgcgca ttctcgatca tgacgatagc
     7261 ggcatcgacc atcgccccga cggcaatcgc aatgccaccc agcgacataa tattggcatt
     7321 cagcccctgg aagggcatga caataaaagc aatacacaac cccagcggca acgaaataat
     7381 cgccaccagc gccgagcgca catgccagag aaacagcgcg cagactaccg ccaccacaat
     7441 aaactcttcc agcaacttgc cgctgaggtt gtcga
//
LOCUS       DAJVPP010000156         7449 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVPP010000156 DAJVPP010000000
VERSION     DAJVPP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.156"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(28..2940)
                     /locus_tag="OQM34_003734"
     CDS             complement(28..2940)
                     /locus_tag="OQM34_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1453997.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            2943..3059
                     /locus_tag="OQM34_003735"
     CDS             2943..3059
                     /locus_tag="OQM34_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1453998.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3423..4265
                     /locus_tag="OQM34_003736"
     CDS             3423..4265
                     /locus_tag="OQM34_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1453999.1"
                     /translation="MLNEGGILFKANHIPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4268..5356
                     /gene="capU"
                     /locus_tag="OQM34_003737"
     CDS             4268..5356
                     /gene="capU"
                     /locus_tag="OQM34_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1454000.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5361..6311
                     /gene="virK"
                     /locus_tag="OQM34_003738"
     CDS             5361..6311
                     /gene="virK"
                     /locus_tag="OQM34_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCS1454001.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6376..7320
                     /gene="lpxM"
                     /locus_tag="OQM34_003739"
                     /gene_synonym="msbB"
     CDS             6376..7320
                     /gene="lpxM"
                     /locus_tag="OQM34_003739"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1454002.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2019 a         1645 c         1739 g         2046 t
ORIGIN      
        1 tcggttacat cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc
       61 cacccagtat ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat
      121 atccactaat tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc
      181 cggtgactgc aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg
      241 gttaagaata tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt
      301 gctgttaact tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc
      361 cggcagcaac gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc
      421 ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac
      481 cagaaacatt gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga
      541 cagactttca ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg
      601 cgtactgacc gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag
      661 caagggatct gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc
      721 tgctgacatc agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat
      781 atcccgcaac acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag
      841 taactgcaga gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc
      901 attatacagc gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt
      961 tttatcggct gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat
     1021 aatctgccat ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc
     1081 ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat
     1141 ctcttcctgc agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg
     1201 atgctccacc tgctgttcaa caagatacac attgtgtgta gcacgggtga gcgcaacata
     1261 cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg
     1321 ggaataacgg gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa
     1381 tgcctgccgg gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt
     1441 aacctcctga accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg
     1501 ttccggatgc atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc
     1561 ctgctttgtc tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct
     1621 tctgacaaaa tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag
     1681 caaccggtta gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt
     1741 aacggttgtg tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga
     1801 gaaaaagctc ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg
     1861 acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac
     1921 aaaaatgaca tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact
     1981 gtcatactga tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt
     2041 cgggcggtcc tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg
     2101 ggacagcggc ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag
     2161 ggtatgggca gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc
     2221 actgaaagtc acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga
     2281 aagaaagtca atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc
     2341 tttttccacc agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat
     2401 tttttccagt accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag
     2461 cgggtgttcg tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg
     2521 gaaacgccca tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc
     2581 cggcactggt tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg
     2641 tgaagtgtta taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg
     2701 gtagcgatac agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt
     2761 ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa
     2821 ttttttataa cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc
     2881 tgccggtttc cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa
     2941 ccttgcccgg aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat
     3001 ggaataaaga aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc
     3061 tgcaggcctg tcatgatgcc ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc
     3121 cgtaaataaa ctctgcgctg aaattccttt ttttcattgg agcacctgtg ttgttctgag
     3181 gtgaacatat cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt
     3241 gagtcgtttt taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt
     3301 agtaaattat atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata
     3361 aaatgcacca gctttattgg gttttccttt tgtctctgct tactaggtgc cagaaacttt
     3421 aaatgttaaa cgaagggggc attttgttca aagccaatca tattccggta ttaatgtatc
     3481 atcatgtcag tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa
     3541 taaaatggtt ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt
     3601 atcggggcgg aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg
     3661 acaactggtt tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt
     3721 taattaccgg ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc
     3781 atcgcgattg cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt
     3841 ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc
     3901 atacacgctg ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg
     3961 acttgttgag cgggagagaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt
     4021 gttggccgga aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct
     4081 attatttata tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag
     4141 gaagaataag caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt
     4201 atacaacccc gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg
     4261 attaataatg aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca
     4321 ggctgttgct caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag
     4381 ggaaaacagc aaaattgctt ttgaagccag taaattgggg attgatatca cattcgcgtt
     4441 atttcgaaac agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt
     4501 tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg
     4561 gttattcact aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa
     4621 aacaaaagtt ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat
     4681 gaagacacat ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt
     4741 tgatttccag aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg
     4801 gctggcgtcc cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa
     4861 agggcacgaa tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg
     4921 ttggttgatt gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga
     4981 cagtatgggc atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt
     5041 atatcgggtt gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact
     5101 ggcagaagca tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga
     5161 tgttattcag aataaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat
     5221 gtgcgccctg aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc
     5281 aaaacaggat atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt
     5341 agcgaagcac aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta
     5401 aaaggattgt tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt
     5461 tcatgtacat tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac
     5521 tgcgttcctt gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa
     5581 ttcatcgacc ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg
     5641 agcattatcg ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa
     5701 aatcggtatc attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt
     5761 ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aataaaatag
     5821 tgattgcccg gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg
     5881 gcggattaca gggagccccc aaaaataccg gacctgatgt tattcggtgc gcaacccggg
     5941 cctgttatgg gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag
     6001 cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga
     6061 gatactggta tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg
     6121 tagcagggaa aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc
     6181 taagcaatat agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt
     6241 atatccatga aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg
     6301 acttaaatta aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg
     6361 cattcgcact aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat
     6421 tatctttccc ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca
     6481 atgtttcccc cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga
     6541 ttgagcagaa aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag
     6601 agtgatacag aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata
     6661 gtgatgatgg cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg
     6721 aaaggcctcg aaattcttga ggaaattcgg cacaataaca gaaatgtgat ttttctggtt
     6781 ccccatggct ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg
     6841 gccgccatgt ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg
     6901 cgtaaattcg gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca
     6961 gtacgccagg gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt
     7021 gaatttgctg atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg
     7081 aacatcagtc aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc
     7141 ctgactattg aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg
     7201 attgcccgac aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat
     7261 atctgggttt taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga
     7321 aattataaag tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat
     7381 cccccttccg tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt
     7441 taatttatg
//
LOCUS       DAJVPP010000157         7437 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVPP010000157 DAJVPP010000000
VERSION     DAJVPP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.157"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..987
                     /gene="yjeM"
                     /locus_tag="OQM34_003740"
                     /pseudo
     CDS             1..987
                     /gene="yjeM"
                     /locus_tag="OQM34_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1138..4449)
                     /gene="mscM"
                     /locus_tag="OQM34_003741"
     CDS             complement(1138..4449)
                     /gene="mscM"
                     /locus_tag="OQM34_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1454003.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4471..5439)
                     /gene="psd"
                     /locus_tag="OQM34_003742"
     CDS             complement(4471..5439)
                     /gene="psd"
                     /locus_tag="OQM34_003742"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1454004.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5536..6588)
                     /gene="rsgA"
                     /locus_tag="OQM34_003743"
     CDS             complement(5536..6588)
                     /gene="rsgA"
                     /locus_tag="OQM34_003743"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1454005.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6683..7228
                     /gene="orn"
                     /locus_tag="OQM34_003744"
     CDS             6683..7228
                     /gene="orn"
                     /locus_tag="OQM34_003744"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1454006.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1686 a         1954 c         2007 g         1790 t
ORIGIN      
        1 tctgaattta gtattgctgt tagtaagcat tactattttg ttattaaatg gtgggcattt
       61 cgcgcaggat attaatttcc ttgcatcacc gaacccgggt tatcagtccg gtctggcaat
      121 gctatcgttt gtggtatttg ctatttttgc ctatggcgga attgaagcgg ttggtggtct
      181 ggtcgataaa acggaaaatc cagaaaagaa ctttgccaaa ggtattgttt ttgccgctat
      241 tgttatttca atcggttatt cgctggcaat atttttatgg ggcgtcagca caaactggca
      301 gcaggtatta agtaatggtt ccgttaacct tggcaatatt acctatgtgc tgatgaagag
      361 cctcggggtg acgctgggta acgcactgca tttgtcacct gaagcgtcat tgtcgctggg
      421 cgtatggttt gcgcgtatta ccggactttc gatgttcctc gcttataccg gtgcgtttgc
      481 tattcaccgc tgaaagccat catccagggg acgccgaaag cgttgtggcc ggaaccgatg
      541 acgcgcctga atgcgatggg gatgccgtct atcgccatgt ggatgcagtg cgggttggtt
      601 actgtcttca ttctgctggt ttcgtttggt ggcggtaccg catcggcgtt ctttaacaag
      661 ctgacgctga tggcgaacgt gtctatgacg cttccttacc tgttcctcgc gctggctttc
      721 ccgtttttta aagcacgtca ggatctcgac agaccatttg tgattttcaa aacgcgtatg
      781 tcggcaatga ttgcgacggt ggttgtcgtt ctggtggtga catttgcgaa cgtcttcacc
      841 attattcagc ctgtggttga agccggagac tgggacagca cattgtggat gattggcggc
      901 cctgtcttct tctcgctgtt agcgatggcg atttaccaga actattgcag tcgcatggcg
      961 aataaacctg agttagctct cgactgatca tatgaaatcc tgcaactcat cgtctctccg
     1021 gcgatgagtt gtcaggaagt aatgcgtatg gatgatactc tccagggccg gtgataactg
     1081 aagatgatgt taagctgcta aatgagccac gttgacatca tttggcagct tcatgcatta
     1141 caaacttccc gcctgacgac ctttgcccgc agacgtcagc gttctccccg tttgtttacc
     1201 gttgaggctt tccagacgca tctggaaggg cgggaatggc atatcgatac cgtgggcgtg
     1261 gaagcctgcc agaatcagct ggtggatctc atggcgtagc ggcatacggt gacccatctc
     1321 agcggcgtaa atacgcagct cgaaaatctg aatcccctgt tgcagatcca ccaggaagac
     1381 ttccggtgcc gggttgtcga tcaccagcga gcagcgacgc gctgcggtga gcaggatttc
     1441 cgtcacttct tcactgttgg catcggcagg ggccggaatc gtcaacacta cgcgcgtgac
     1501 cgagtcagag agcgaccagt tgataaactg ctcggtaata aacgccttgt tcggcacgat
     1561 tatctcttta cggtcccagt cgctgatggt ggtggcgcgg gtgttaattt ttgtcacgct
     1621 accagtaaga tcgcggatcg ttaccgtatc gccaatgcga atcggttttt cgaacaggat
     1681 gatcaggcca gagataaagt tggcgaaaat ttcctgcaaa ccaaaaccga gaccaacacc
     1741 gagcgcggca accagccact gcaatttcga ccactcaata ccaatcattg agaagccgac
     1801 cagcccgcca atcagcatca gcagatattt tgtgatggtg gtgatggcgt aacccgtacc
     1861 cggcgttaaa tccaggtgct gcaaaatcgc cagttccagc agcgcgggca ggttgcgcac
     1921 cagctgcgtg gtgatgataa ataccagaat ggcaatcagc accgcaccga gggtaattgg
     1981 ctccagactt tctacgccct gtaccgtgga ggtgacatcc cacagcgaaa tattttcgag
     2041 gaagccgaaa gcggaatgga tttctgacca cagcacaatg accgaaagca gggcgatcaa
     2101 catcaaaatt gagcgcacca gccgcaagga ttgcgcactg atggcatcga gatcgacttc
     2161 gctttcatcg acttcaattg ctccttccgg gctactgtga tgatgcgctt cctcttcgcc
     2221 acgcgcacgt tgcgctaaca tctctgcccg gcgatgcttc gcccggtcaa acgccagcct
     2281 gcgacgctgg atcagcatcc agcggcggat aacgtgataa accaccagta gcaggaacca
     2341 gatggcaacc gaggtttcaa gcctcgctaa cagtgcctgc gccgttgcca gataacccac
     2401 cgccgacgcc agaatggcaa ccagtggtgc gccaatcatc atgttccaca gcatatggtt
     2461 ggtaatgttg tcgccgctgc cttctttgtt gagatacaac ggaataccgg cttttttcag
     2521 gcttagggtg accaccgcca gcgcaccgca aatgaggata aagcaaagcc gtcccagcga
     2581 accggaaaat tcgcggtcgt cgaggttatc gaacatcatc agcgccataa tcagcccgat
     2641 gctcatcagg tagtagcgca tcccacggga aacacgttcg cgcggccagc caaaatgagc
     2701 gataaacaag ccgttcgggc gggcaaaggt ggcgcaaatc atcactaccc acagcagcgg
     2761 cacggtggcc gttacaccat cgccaatcgc gaccgccagc ggataaggcc acgcctcgcg
     2821 caagccgtaa cccagcgtca tccacagcac cggtaacggt gacgcgacga gaatcgacca
     2881 gaaaagagtg cgcaacgtca gccagaagtg atcctgagtc actttgccga ctttcgccgc
     2941 cgaacgttca agaaaacggg tgaaatagcg gcgcgagtaa atactgcaac cgaccagaat
     3001 caacgcgcca aacagcggca aaattgtctc tttgctggtc agcatcatca cactggcttt
     3061 gcccaactga ctgaaggtgt ccagcgaaat gagacgacgc agatcctggg cgatttccag
     3121 cggccaggcg atggtcatcg ggcgcacgtc agaggcccag aacagatagc ggtgcgttgc
     3181 ttcgttcacc tctttcagcg catcctccag ttgcccgttg gagactttca gcttggtcag
     3241 ttccaatagt agcgtgtcgc caccctgcaa caatgagttc agcaactcac gctgagtgcg
     3301 cagttgtgct tccagaatac ggttttgctc ggcggtcagc ggctgaccgt cggcctgatg
     3361 gatttgccgt agcagcggct gtttattaag taaatcctca taacgtaacc gttgcacacg
     3421 caactgcgcc atttcggtat caagctgttg tggttttggc atttctggta gccgtgccac
     3481 ctgcgcccgc agcgcttcgc cgagcaggtt ggacgatccc agccattgcg actgttcacg
     3541 cagcgtattc aacgcctgcc ggacctgtaa cgtctggctg gcagcctgac gctgttgcga
     3601 ggcaacgagc tccatccgct gcgcctgttg attcagcgct gccgatagtt cgcggttaat
     3661 tttgaattgc gcgacgatat ctttcggcaa atcggcactg ttttctgcca gtaattcggt
     3721 actttccagc gcccgctccg cctcaagctg acgttggcta ttcaactgat tacgtaaggc
     3781 ctgcaaatac gcatccagtt gctggctctc tttttccgcc agctctgagc gtaagcgcgc
     3841 taattcctgg cggttattgg cagacagctg cgccagctcc agttcatcaa cgagcgcctt
     3901 aagacgtgca gagtcagact gcaacgcgaa attttgtgcc tgattgagcg gagtattgcc
     3961 ggtaagcgtt cccaggcggc gctcgatctc atttaactga cggcgggcgt cggtttgctg
     4021 ttgcggcagt tgattcagcg aatcggcaat ctcgcgggcg cgctcctgct cttgctgggc
     4081 ctgacggctt ttatccagca actggctgct gacctggaga atttcctgat tcagcgcgtc
     4141 ggtagacatt cccggcgaca cgctgcgcgg ctcgtcacga atgttgttta attgtgcgcg
     4201 cagagtagcg gagagtttcg gataattatc gataacttgc tgatattgtt tgatgcgctc
     4261 aagggaacct tttcgttcct caagcgcatt taaggcagac tggagcgcct ctacgacttc
     4321 cggctgtgcg ggtttcgccg cttttgcctg ctccagttcc tgagtgattt gtttgctatc
     4381 gggggccgtc gcggcgtacg ccccccaact gaggcaccag gccatcagaa aagtgataat
     4441 caggcgcacg tcagcgtttc ctttgatgga ttagacctgg tcttttttat cgtcaaccaa
     4501 tgggctggcg tcgtgttctg cttcgatctc ttcagcagga agtggggcgg gttcagcgtc
     4561 tggcgtaaca aaggtttcgg tagatactgc cagcggctgg ccaattttcg tgacagacag
     4621 gctttccagt tgctcaacca gattcacttt acccggtgca aacaggttga taacggtgga
     4681 accgagttta aagcgaccca tttcctggcc tttcagcagt gccacagaac cgtcgttttc
     4741 cccggcaggc caggtccagc gcttgatgat accttcgcgc ggcggcgtaa tggtgcccgc
     4801 ccagaccgtc tcaatgctgc caacaatcgt cgctccgacc agaatctgcg ccattgggcc
     4861 aaattcggta tcgaaaaggc aaatcacgcg ttcgttacgg gcaaacagat tcggcacgtt
     4921 ctgagcggtg agatggttaa cggaaaagag atcgcccggc acgtagatca tctcacgcag
     4981 aataccgttg cacggcatgt gtacgcgatg gtagtcacgc ggggagaggt aagtggtcac
     5041 aaacgtaccg ttgcggaaca ggtccgccat cagatagttg cctgccagca gggcttcgag
     5101 gctgtagttg tggcctttgg cttgcaggat tttatcttct tcgattttac ccaactggct
     5161 gataacgcca tcggcaggca tgaccagtac attcggatcg gtatcgattg ggcgtacttc
     5221 gtcacgcagc ggacggacaa agaattcgtt aaaggtgcgg tagctggcgg tgtccggctt
     5281 ttgcgcctct ttcatgtcga ccttgtagta tttaacgaac agatcgataa ccagttttgt
     5341 cagccatcct gcccgcttgc ttgcgcccca acccgccagg cgagtaagcc atagtttcgg
     5401 cagaatgtac tgtagcgaaa gtttaaatga atttaacaag gtagcctcca ggccattgtt
     5461 ttgtcgttcc tgatccggcc aacatgccgg atcctgaaaa aaaggggacg attctaacga
     5521 cggttagctt aattgtcagt catccgtatc agaaaagttt ttacgcgttt ttacctgcgc
     5581 catgctttcc agaatacggt gatagttttc gaaacgggtt tccgcgattt tcccttcctc
     5641 aaccgcttcc cggatagcgc agcccggatc ggtatcgtgt ttgcaatcgc gatatttaca
     5701 cagacctaaa tagtcatgga attcgacaaa gccctgagtg atttgttccg gctccaggtg
     5761 ccagaggccg aactcacgca caccagggga atcaatcaca tcaccgccgt gcgggaagtg
     5821 atacagccga gcggcggtcg tggtgtgctg accgagcccc gagttgtcag agacatcgtt
     5881 ggtcaggatc tctttttgca gccccagcaa cgcgttcagc aggctggatt taccgacgcc
     5941 agactgcccg gcaaaaatgc tgatgcgccc ggtcaacgcc tcttccagcg gtttcagccc
     6001 atcctgagta tggctggaaa ccatcaatac gcgataaccg atgttgcggt agatatccat
     6061 ctgctcgttg acgaacgcca tgccttcgtc gtccagcagg tctatcttgt tgagcacgat
     6121 aatcggctca atctgcaagg tttcgcaggc caccaggtaa cggtcgataa tattgagcga
     6181 cagctccggc aaaatggcgg agacaatgac aatctggtcg atgttggcgg ctatgggttt
     6241 cacgccgtcg tagaaatccg gacgcgttaa caccgaggtg cgctcatgca ccgcttccac
     6301 gatccctttg acatttacgc cttccgccgc cggtttaccc ggacgccaga ctacgcggtc
     6361 gccggttacc agcgaacgga tggtacggcg aatattgcag cggtgaacgt cgccatcggc
     6421 ggattccaca tcagcgtgca taccaaagcg gctgatgacg ataccttcat caggctcgcc
     6481 aaacagattg tcgtcgtagt cgggcttctc cttagacgtt ttaagacgac gctggtgatt
     6541 ggcgttcacg cggcgctgct ggcctttgga gagtttattt ttactcaatc gtactgactc
     6601 ctggtcgccc ctgatgggca aaacatctat gatacacgca attgtggatc aatatagtca
     6661 ctgtgaatgg gtggaaaata gcatgagtgc caatgaaaac aacctgattt ggatcgatct
     6721 tgagatgacc ggtctggatc ccgagcgcga tcgcattatt gagattgcca cgctggtgac
     6781 cgatgccaat ctgaatattc tggcagaagg gccgaccatt gcagtacatc agtccgatga
     6841 acagctggcg ctgatggatg actggaacgt gcgcacccat accgccagcg ggctggtaga
     6901 gcgcgtgaaa gcgagcacga tgggcgatcg ggaagctgaa ctggcaacgc tcgaattctt
     6961 aaaacagtgg gtgcctgcgg gaaaatcgcc gatttgcggt aacagcatcg gacaggaccg
     7021 tcgtttcctg tttaaataca tgccggagct ggaagtctac ttccactacc gttatctcga
     7081 tgtcagcacc ctgaaagagc tggcgcgccg ctggaagccg gaaattctgg atggttttac
     7141 caagcagggg acgcatcagg cgatggatga tatccgtgaa tcggtggcgg agctggctta
     7201 ctaccgcgag cattttatca agctgtaaaa tttttcctgg tcacgtaagc gccggaatcc
     7261 acttgcggcg gcgtgatttt gacgctaaat cgaacgtctt gctgataatt tcagcacttg
     7321 agataaaaac gcaaaaaaaa cttttttggg gggttgcaga gggaaagatt tctcgtataa
     7381 tgcgcctccc gtaacgacgc agaaatgcga aaattacgaa agcaaaatta agtagta
//
LOCUS       DAJVPP010000158         7428 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVPP010000158 DAJVPP010000000
VERSION     DAJVPP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.158"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OQM34_003745"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQM34_003745"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OQM34_003746"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OQM34_003746"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS1454007.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OQM34_003747"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OQM34_003747"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1454008.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OQM34_003748"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OQM34_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OQM34_003749"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OQM34_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1454009.1"
                     /translation="MILDTVDAKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6575
                     /locus_tag="OQM34_003750"
     CDS             5070..6575
                     /locus_tag="OQM34_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCS1454010.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVGLNHLYGCGVA
                     INAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKLQPERLLTGTDDVQAIPVEIASI
                     VSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRETCPASELVVGMHCGGSDAFSGVT
                     ANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTPRAVNEEVGKRLLEEMEWYDNYL
                     NMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSPGQRPTKRGLIYAA
                     TPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWFDLMDINA
                     GTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQWGLHNQLAVFNPAPVT"
     gene            complement(6627..7418)
                     /gene="agaR"
                     /locus_tag="OQM34_003751"
     CDS             complement(6627..7418)
                     /gene="agaR"
                     /locus_tag="OQM34_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCS1454011.1"
                     /translation="MTGTSERREQIIQRLRQQGSVQVNDLSALYGVSTVTIRNDLAFL
                     EKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVELIQPGHRVILD
                     SGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLRRQSQSFYGDQA
                     EQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERIIVVTDSSKFNRS
                     SLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
BASE COUNT         1806 a         2048 c         1822 g         1752 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaatg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt ttgcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatccctg aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggggc
     5521 tgaatcattt gtacggttgt ggcgtggcga ttaacgcacc ggcagcagtt gtacctattc
     5581 gtaccattca caatatttcg ctgaacccta actttggcgg cgaagtaatg gtgattggcc
     5641 tgggttgtga aaagttgcag cctgagcgcc tgctgactgg aacggatgat gtgcaagcta
     5701 ttccagtaga aatcgccagc attgtcagtt tgcaggatga aaagcatgtc ggttttcagt
     5761 ccatggtcga ggatattttg caggtcgccg aacgccatct acaaaaactg aatcaacgtc
     5821 agcgagaaac ctgtccggct tcagaactgg ttgtcggcat gcattgcggt ggcagcgatg
     5881 cgttttctgg cgtaacggca aacccggcgg ttggctatgc gtctgatcta ctggtgcgct
     5941 gcggcgcaac ggtgatgttt tcagaagtca cggaagtgcg tgacgcgatc catctgctga
     6001 caccacgcgc agtgaacgaa gaggtcggca aacggctgct ggaggagatg gagtggtacg
     6061 ataactatct caatatggga aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga
     6121 aaggcggtct ggcaaacgtg gtagagaaag cgctcggctc cattgctaaa tcgggcaaaa
     6181 gtgcaattgt tgaagtgctg tcgcccggtc aacgcccgac taaacgcgga ttaatttacg
     6241 ccgcgacgcc agccagcgat tttgtctgtg gcacgcaaca ggtggcttcg ggtatcaccg
     6301 tgcaagtgtt tacgaccggt cgtggtacgc cgtacggcct gatggcggta cccgtcatta
     6361 aaatggcaac ccgcaccgag ctggcgaacc gctggtttga tttaatggat attaatgcgg
     6421 gcaccatcgc taccggcgaa gaaactattg aagaggtggg ctggaagttg ttccacttta
     6481 ttctcgacgt cgccagcggg aagaagaaaa ccttctcgga tcaatgggga ctgcataacc
     6541 agctggcggt gtttaaccca gcaccggtga cctgataatt caattcgtat tatgcccgat
     6601 agcacacgtt tatcgggcat attgagtcac agcgaagacg cgtgatcccc gaccagaatc
     6661 acttcaaccc cagcctttcg cagtccttcc agactatccg caggaatgcc ttcatcaaca
     6721 atgatcatgt cgatacgttg agtatcaatg atcttatgta aactggaacg attgaattta
     6781 ctggaatcgg tgaccacgat gatccgttcc gcaacttcgc acatccgacg gtttaaacgg
     6841 gcttcatctt cattatgtgt gctgacgccg cgctccagat cgatcgcatc tacaccaaga
     6901 aacagcatat cgaagtggta attttgcagc gattgctcag cctgatcgcc gtaaaaagat
     6961 tgcgactgac ggcgcaaatg cccgccggtc atcagcagct caacgccttc cgcttccagc
     7021 aacgcattag ccacgttcat accgttggtc atcgcaatta cgtcagtgta cttgcgcatc
     7081 agacgagcaa tctcaaaagt ggtggtcccg gaatcgagga tcacccgatg acctggctga
     7141 atcaactcaa cggcagcttt cgcaacgctg cgtttcatcg cggtgttcag tgcgctttta
     7201 tcttccactg atggctcgac tgacggcgtc gtgctatcgc agatcaacgc gccaccatag
     7261 gcacgcacag cgatcccctg cttttccaga aacgccagat cattgcggat cgtcacagta
     7321 gatacgccat acaatgccga cagatcgtta acctgcacac tcccttgctg tcgcagacgc
     7381 tgaatgatct gttctcgtcg ctcgctggtg cctgtcactc gcttctca
//
LOCUS       DAJVPP010000159         7416 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVPP010000159 DAJVPP010000000
VERSION     DAJVPP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.159"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..460)
                     /gene="hprS"
                     /locus_tag="OQM34_003752"
     CDS             complement(<1..460)
                     /gene="hprS"
                     /locus_tag="OQM34_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1454012.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNM"
     gene            complement(460..1131)
                     /gene="hprR"
                     /locus_tag="OQM34_003753"
     CDS             complement(460..1131)
                     /gene="hprR"
                     /locus_tag="OQM34_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1454013.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1264..1677
                     /gene="hiuH"
                     /locus_tag="OQM34_003754"
     CDS             1264..1677
                     /gene="hiuH"
                     /locus_tag="OQM34_003754"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1454014.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            1786..2790
                     /gene="msrP"
                     /locus_tag="OQM34_003755"
                     /pseudo
     CDS             1786..2790
                     /gene="msrP"
                     /locus_tag="OQM34_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000740079.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            2791..3426
                     /gene="msrQ"
                     /locus_tag="OQM34_003756"
     CDS             2791..3426
                     /gene="msrQ"
                     /locus_tag="OQM34_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS1454015.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            3684..4334
                     /gene="zinT"
                     /locus_tag="OQM34_003757"
     CDS             3684..4334
                     /gene="zinT"
                     /locus_tag="OQM34_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1454016.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(5412..5681)
                     /locus_tag="OQM34_003758"
     CDS             complement(5412..5681)
                     /locus_tag="OQM34_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1454017.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            5738..6406
                     /locus_tag="OQM34_003759"
     CDS             5738..6406
                     /locus_tag="OQM34_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1454018.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            6722..>7416
                     /locus_tag="OQM34_003760"
     CDS             6722..>7416
                     /locus_tag="OQM34_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1454019.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2151 a         1663 c         1501 g         2101 t
ORIGIN      
        1 gcatattatg tctggctgaa gccaatttag ccacagtaac cgttaatgat ggcgaaactt
       61 catcaatgtt aattcgtaaa gcatctatct ctgtatcatt aataatgctt ctgtaaatgc
      121 ctgctgcgct gattgtctca ctagcaggta tgttatttaa catgtcatca ctgacatttg
      181 tccggttaac aattttattg atgccatcac tatgaatgat caaaatatcc tgactaacat
      241 ccatcatccg gttaaagtac acaggtaacg tatctggatt taccccatca attaacaact
      301 gcttgatctg cgctgtccgg ttaatgagtg ttgtatcatc gcgccatttc aactcacttg
      361 ccaggccatt atagagagtc catacaattc cagcgccagc aacagactgc agaaatataa
      421 aaagcaaggt taaacggacg gttatagata atcttttcat tattttttta ccgctacgaa
      481 tgaatagccc atcccccgga ttgtggcgat tagcttttca ggaaaaggat catcaacttt
      541 tgcgcggagc ctgcgaatgg cgacatccac cgtattggta tcactatcaa agttgattcc
      601 ccaaatttca ctcgcaataa ccgttctggg tataatctcg ccagctctgg aggccagtag
      661 ccaaagtaac tgaaactcct tgcgcgtcag tgtaatactg atattgtccc tgctgacact
      721 ttgactgaca gagtccattt ttaagccact gatttctaat gttgaattca gagcgtgatg
      781 ttgccttaat tgtgcccgaa cccttgctag caactcagaa aatgaaaaag gttttaccag
      841 ataatcattt gccccactgt ccaaccctct gactctgtca tcgacagaat cccttgcagt
      901 aaggcaaata acaggggttt gctttgccgt tcttaacgtt tgtaagatct gccagccatc
      961 catacccgga agcataatat ccagaatgat caatgcataa tcatccttca gcgcaagata
     1021 aagcccatct ctgccatcag aaacggcatc aatgacataa cccgcttcgg aaagcccctg
     1081 cgttacccat tcctgggtcc tttgattatc ttcaataagt agaatcttca tgaaaataat
     1141 atgccataaa tcatatgtta tagaaacaac ctggttcatt acaaaattgt aatgctgctg
     1201 taaggttacc ctggacgttt tttcgctatc ctcaaaactc attcacatga caaggatata
     1261 aacatgttaa agcgttattt agtactctcg gtagcaacgg cagcattttc attaccttct
     1321 ttggtttatg ccgcacaaca aaacattctt agcgtgcaca ttttgaacca gcaaacagga
     1381 aaacccgctg ccgacgtgac agtcactctt gaaaagaaag cggataacgg ctggttacaa
     1441 cttaataccg ccaaaacaga taaggatgga cgaattaagg cactgtggcc cgagcaaact
     1501 gcgactactg gcgattaccg tgtcgtattt aaaaccgggg actatttcaa gaaacaaaat
     1561 cttgaaagtt tcttccctga gattcccgtt gaatttcata ttaataaagt gaacgagcat
     1621 tatcatgtgc ctttgttact cagccaatat gggtattcaa cctatcgtgg cagttaatgc
     1681 gtaaatatgt aaaagctgat ctctgctact gttagggagg tgactccctg acagattaac
     1741 agtaaacagc tcttgttggt taacgacaaa aaagtgtgat ggcttatgaa aaagaagcaa
     1801 tttttaaaag aatcagatgt tacggccgag tcggtattct ttatgaagcg tcggcaggtg
     1861 ttaaaagcac tgggcatcag cgcagctgca ttttctttgc ctcacgctgc gcatgccgat
     1921 ctgcttagct ggtttaaagg gaacgatcgt ccgcccgccc ccgccggaaa accgctggag
     1981 ttcagcaagc ctgccgccta gcaaaataac ctgccactga cgccagcaga taaagtctcc
     2041 ggttataaca acttctatga attcgggctg gataaagacg atcccgcggc taatgctggt
     2101 agcctgaaaa ccgatccatg gacactgaaa atcaacggcg aagtggcaaa accattgacc
     2161 ctcgatcatg atgatttaac ccgtcgcttc ccgctggaag agcgtattta tcgtatgcgc
     2221 tacgtggaag cgtggtcgat ggtggtgccg tggattggtt ttccgctaca caaattgctg
     2281 gcgcttgccg aacccaccag caatgcgaag tatgtcgctt tcgaaacgat ttatgcgccg
     2341 gaacagatgc ctggccagca ggaccgcttt atcggcggcg ggctgaaata tccttatgtc
     2401 gaaggattgc gtctcgacga agcaatgcat ccgctcacac tgatgaccgt aggtgtttat
     2461 ggcaaggcgt taccaccgca aaatggcgcg ccggtgcgac tgattgtgcc gtggaaatat
     2521 ggctttaaag ggattaaatc gatcgtcagt attaagctga cccgcgagcg tccgccaacc
     2581 acctggaatc tggcagcgcc tgacgaatac ggtttttacg ccaacgttaa tccgcatgtt
     2641 gatcacccgc gctggtcaca ggctaccgaa cgatttattg gttcaggcgg cattctcgat
     2701 gttcagcgcc agccaacact actgtttaat ggttacgccg accaggtggc atcgctgtat
     2761 cgtggcctgg atttgcggga gaatctctaa atgcgtctga cagcaaaaca agtgatatgg
     2821 ctgaaagttt gcctgcatct tgccggattg ttgccgtttc tctggctggt ctgggcgatc
     2881 aatcacggtg gactgggtgc cgatccggtg aaagatattc agcattttac cggtcgcact
     2941 gcactgaaat ttttgcttgc ggccttgtta atcacacctc tggcacgcta cgcaaaacag
     3001 ccgttattga tacgcactcg ccgcctgtta ggcttatggt gctttgcctg ggcgacactg
     3061 catttaacca gttacgcatt gctggagttg ggcgtgaata atctggcgtt attaggtaag
     3121 gagttaatta cccggcctta tttaacgtta ggaattatca gctgggttat tttgcttgct
     3181 ttagcgttca cttcaaccca gtcgatgcag cgaaaactcg gcaaacactg gcaacagttg
     3241 cataacttcg tctatcttgt cgcgatcctg gccccaatac attatctgtg gtcagtgaag
     3301 attatttcac cgcagccact catctacgct gggctggctg tactgctttt agccttacgg
     3361 tataagaagt tgctttccct tttcaaccgg ttacgcaagc aggcccacaa caaattatct
     3421 ctataacttc atattgccga caaacttcgc gttatcccgg ccggatgcgg cgcgagcgcc
     3481 ctatccggcc cacacagatc gcatcaagtt atgtttcgtt tgtcatcaac ctttcatcac
     3541 cagaataatt tattttattt caataagata gttcaataca cctgagaata ccatgctctc
     3601 atttcctaat gagttgttgc attttttcat atgttacaat ataacattac acataatgta
     3661 cattaactct ggaggaaatt gttttggcga ttcgtcttca taaactggct gttgctttag
     3721 gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcattctcat ggcaaaccct
     3781 taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgataccaat gtacaaaacc
     3841 gaacgctcag tgactgggat ggagtctggc aatccgttta ccctttactg caaagtggca
     3901 aacttgatcc cgtctttcag aagaaagcgg atgcagataa aaccaaaaca tttgctgaaa
     3961 ttaaagatta ttatcgcaaa ggttatccga cagatatcga aatgattggc attgaggacg
     4021 gcattgtcga attccataga aataatgaaa caacatcctg taaatatgat tacgatggat
     4081 acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc gaatgtaaag
     4141 atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt gcaccacgaa
     4201 aatcatctca ttttcacatt tttatgggta atgattccca gcaatcttta ttgaatgaaa
     4261 tggaaaactg gccaacgtat tatccctatc agttgagtag cgaagaagtg gtcgaggaga
     4321 tgatgtctca ttgatttttt tgcgctgact tcagttattt aacagaacag tgcctggtag
     4381 taacaacaat aatttaccaa gacaaataag taagacaaac tcagacacgc tcatacttga
     4441 gtcgtgtctt ttttattatg tctgtagaaa aaaactgaga aaaaggctaa caataaatac
     4501 acaactcagc catacagact ggcagcgcaa atgtttccag gagcaagtca tctgtcatgg
     4561 taaagctaac cagaaatata ggttaatttt ggtgtttaaa ccttttttac ttgttagtat
     4621 gatacaaagg ctttcaaaaa agctgggaaa ttcaacaact cttggaaatt atggactttg
     4681 tcccgcggct ttaatctgac ggccgcgttc cttttttggc tcaattattg accccttctc
     4741 tacgcaactt cagttcccgc cagcaacttt gcggcagttt cataggatca atgtctaaaa
     4801 gaataatggt gaccgataag aaaacgactg aataactgta gattttcgct cgaaaccttc
     4861 ctgtcagctc catagcgaat caagcgctga atgtcacagt atcgaacaga aaacagtgac
     4921 gatctaaccc ttcaagaata ttctacgatt gttctgttta ggaaaagcaa ggcgggaagt
     4981 cgggagataa gtcattgata aaatggcgga gagaggggga tttgaccccc cggtagagtt
     5041 gcccctactc cggttttcga gaccgatgat acagagtcga aaaatcaaag agttaaaaat
     5101 ttttcttgga aaaaatgtct taaaatcgta cacgaaaaat caatgaatta cataacatta
     5161 aagaagaata ttccaagcct tcttgagtcc accacctgtt ttcaggtgtc attcagacgt
     5221 gatttgcccc agattgggaa agcacgcttt tttctcagct tattccgttt tgcctgtgtg
     5281 ttgaataatt ccactgcagt tatataaaat cggtaacggc tggaaatcat tcaatactcg
     5341 cactatcgaa agtttaccag ccaaccgcag cacgtcctgc atacgtcgtg tctgcggttt
     5401 ttctttttcg cttacattgt gtctggttct tccggccact caatatcagg tgcagttgat
     5461 gtatcaacac ggttcagcaa cacccgatac ttcttccagg cttccagcaa cgagttttct
     5521 tcctccgttg cgatctccag atctacagca tcctgaagtg gcgcaatatg ctcactgaat
     5581 ccctggatgt agaactgtgt ggtgacggtc ttccagccat tcggctcctg ctgtatcgaa
     5641 gcataccagg ctatttcaat atcgctatgc tgcggcagca tttaacccct tgtaattcat
     5701 cgccataatt gatttaattc acaaataaaa ctataatatg gtgaaattaa tgaaaaaaaa
     5761 cacagatgat ggggctaaaa tttacacacc acttacccta aagctttatg actggtgggt
     5821 tttgggagta tcaaatcggc ttgcatgggg atgtcctaca aaggaacacc ttcttccaca
     5881 ctttctggaa catttaggta acaaccatct ggatattggt gttggaactg ggttttacct
     5941 tactcacgta cctgagagta gtctgatatc tttaatggat ttgaacgaag ctagcctgaa
     6001 cgcggcatct acaagggctg gggaatcaaa aattaaacat aaaattagcc atgatgtttt
     6061 tgaaccttat cctgcggcgt tacatggtca atttgattcc atttccatgt tttaccttct
     6121 tcactgcctg cctggaaata tatctacaaa aagctgtgta atacgcaatg cggcgcaggc
     6181 cttaactgat gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa
     6241 tagcttcggt caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa
     6301 agattccgaa gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccaa
     6361 ggttcaaggt actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc
     6421 acgttcttgc ttaagacgtg ctacggtttc ctttacgatt tggggttgcg actttacctc
     6481 tattgataat gcattccggc agaacgttca aatatgaacg tacgatattt aactaaccga
     6541 aaaacaaaat atagcacagg cgagacattt accatctaag aaaatagtat cgttttttta
     6601 cagttaaatc aacatcactt ccttaaaatg aaaacaataa atttcaatcc tgaaacattt
     6661 tatttcagct tattattccc cagtatcaca ttttatcagg atatccttct gcgaggttat
     6721 tatgcttcct gtaaataatc cccccctatc cactggaaac gtctcttttt acagaactac
     6781 atcaatcgac aatgttcaca ataattatct ctccgaatgg gttgaatgga ctaaaaacag
     6841 catttccgga gaaaacaggg aaactgcttt tacccggctc caattatgtc tggagaacag
     6901 tgaaacatcg ttggacttat cttgtttagg tctcagatct ctaccacgat tgcctgacaa
     6961 tcttgatgaa attaatgtaa gcaataacca actatcaatg ctccccgagc taccaagggc
     7021 attgaaagag ctgaatgcaa gcagtaatca attatctgca cttcctgaat taccagtgtc
     7081 gctggaatat ataaatgtga gtgataacca tttgttcgca cttcctgaat tacctgcgtc
     7141 actagaatat attaatgtaa gtgacaatca cctgtctgta cttccgaggt taccaatgtc
     7201 attggaatta cttgatgcag ccagaaatgc tttggaagta ataccagatt ttccagaaag
     7261 agatgatcat attataagaa tattctggct taatcagaac cggatcacgg caattccgga
     7321 aagcatactt ggcctcagtt ctgatagcgt tgtcaatctt agagaaaatc aactatctcc
     7381 cagaataatg caaactttgt tacaacaaac cgccca
//
LOCUS       DAJVPP010000160         7349 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVPP010000160 DAJVPP010000000
VERSION     DAJVPP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.160"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(26..232)
                     /locus_tag="OQM34_003761"
     CDS             complement(26..232)
                     /locus_tag="OQM34_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS1454020.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            545..634
                     /gene="kdpF"
                     /locus_tag="OQM34_003762"
     CDS             545..634
                     /gene="kdpF"
                     /locus_tag="OQM34_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1454021.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            634..2307
                     /gene="kdpA"
                     /locus_tag="OQM34_003763"
     CDS             634..2307
                     /gene="kdpA"
                     /locus_tag="OQM34_003763"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS1454022.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2330..4377
                     /gene="kdpB"
                     /locus_tag="OQM34_003764"
                     /pseudo
     CDS             2330..4377
                     /gene="kdpB"
                     /locus_tag="OQM34_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4386..4958
                     /gene="kdpC"
                     /locus_tag="OQM34_003765"
     CDS             4386..4958
                     /gene="kdpC"
                     /locus_tag="OQM34_003765"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCS1454023.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVHGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4951..7349
                     /gene="kdpD"
                     /locus_tag="OQM34_003766"
                     /pseudo
     CDS             4951..7349
                     /gene="kdpD"
                     /locus_tag="OQM34_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
BASE COUNT         1538 a         1917 c         2135 g         1759 t
ORIGIN      
        1 gaggctggct taagcgcgca ggcaatcagt aaaaatcacc ggttgccttt tcggcctgat
       61 ccatccacac cggtttatcg ctggttttcg accagacgcg gtgcaggtag ctgtaaaagc
      121 gggcgcggtc tttccagaaa agcatgaaag gcagcgccag aacgcccgct gcaaccgcat
      181 atgtacggcg taaaaagata agccatgcag gatattctct gtagagttcc atacttttct
      241 cccccttttg tatctacccg gtgaatagca ccggaaaaat gaatttgttt atctgatgaa
      301 aatagtaccg ccttttgtgt aattttacta ctcatccgac cacttatttt tgcttattga
      361 tggtttattt acattcacac tgtaattaaa ttacacaaaa gttaaattaa tactaaccat
      421 cagttaaatc atggcctttg ccatttttat acttttttta caccccgtcc gcagattttt
      481 gcgaaatctt tgcagccaga attctactct tccggtatca cttttaggcc actggaggtg
      541 cactgtgagt gcaggcgtga tagccggcgt gttgctggtg tttttattac tgggttattt
      601 ggtttatgcc ctgatcaatg cggaggcgtt ctgatggctg cgcaagggtt cttactgatt
      661 gccacgtttt tactggtatt aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg
      721 ctgattaatg acattcctct tccaggtaca gcgggcgttg aacgtatact ctttcgttta
      781 cctggcgtct ctgaccatga gatgaactgg aagcaatatc tttgtgccat tctcggcctg
      841 aacatgctgg ggctggcggt gctgtttttt atgttgctcg gtcagcacta tctgccgctt
      901 aatccacagc agttgccggg gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt
      961 gtcacgaata ccaactggca atcttatagc ggtgaaacca cgttgagcta tttcagccag
     1021 atggcaggct taacggtaca aaactttctt tctgccgcca gcgggattgc ggtgattttt
     1081 gccttcatcc gtgcgtttac tcgccagagc atgagcacac tcgggaatgc ctgggtcgat
     1141 ctgctacgca tcacgttatg ggtgctagtc cctgtggcgt tgttgatcgc actgtttttt
     1201 attcaacaag gtgcgcagca aaactttctg ccctatcagg cagtgaatac cgttgaagga
     1261 gcgcaacagc tgttacccat gggaccagta gcttctcagg aagcgatcaa gatgctcggt
     1321 actaacggcg gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca
     1381 ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc caacagcgct gtgctttgcc
     1441 tttggtgaag tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg
     1501 atttttgtca tctgcgtagg cgtggttatg tgggcagaag ttcagggtaa tcctcatctg
     1561 ctggcgctcg gcgcagacag cagcatcaat atggaaggta aagagagccg tttcggcgtg
     1621 ctggtcagta gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg
     1681 atgcatgatt cgtttaccgc tctcggcggc atggtgccga tgtggctaat gcaaattggt
     1741 gaagtggtgt tcggcggtgt cggttctggt ctttacggca tgatgctgtt tgtcctgctg
     1801 gcggtgttta ttgccgggct gatgattggt cgtacaccgg aatatctggg taaaaaaata
     1861 gacgtacgcg agatgaaact gactgcactg gcgattctgg ttactccaac actggtactg
     1921 atgggcgcgg cgttggcgat gatgaccgac gccggacgta gcgccatgct taaccctagc
     1981 ccgcatggtt ttagcgaagt gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc
     2041 gcctttgccg gattaagcgc caactctccg ttctggaact gtttactggc gttctgcatg
     2101 tttgtcggtc gcttcggggt gattatcccg gtgatggcaa ttgccggttc gctggtgagt
     2161 aaaaagagcc aacccgccag ctccggcacg ctgccaacgc acggcccgct gtttgttggc
     2221 ctgttaatcg gcaccgtgtt gctggttggc gcactgacct ttatccctgc cctggcgctt
     2281 ggtccggtgg cggaatatct ctcctgatga tattgagtga gcactgaata tgagtcgtaa
     2341 acaactggcg ctattcgaac caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa
     2401 attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct
     2461 gctgaccacc tgtattagca tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt
     2521 tagcgcggca attagcggct ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc
     2581 gctggcagaa ggccgcagta aagcgcaggc caacagtctt aaaggggtga aaaaaactgc
     2641 ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg gcggacaaag ttcctgccga
     2701 tcaactgcgt aaaggtgaca tcgtgctggt agaagctggc gatattatcc cctgcgatgg
     2761 tgaagtcatt gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg aatcggcacc
     2821 ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc ggcggcacgc gtattctttc
     2881 tgactggctg gtgattgagt gtagcgttaa ccccggtgag acatttctgg atcggatgat
     2941 cgcgatggtg gaaggcgcac agcgacgcaa aacaccgaac gagattgccc tgactatcct
     3001 gctgattgct ctgaccatcg tctttttact ggcaaccgcc acgctgtggc cgttttccgc
     3061 gtggggcggt aatgccgtga gtgtaacggt actggtggcg ctgctggtct gtctgatccc
     3121 aaccactatt ggtggcctgt tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg
     3181 cgcgaatgtg attgccacca gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct
     3241 gctactggat aaaaccggca ccatcacact cggtaaccgt caggcgtcgg agtttatccc
     3301 cgcgcagggc gtggatgaaa aaacgctggc tgacgccgca caactggctt cgctggctga
     3361 tgaaacgccg gaaggtcgca gtattgtgat cctcgccaag caacgtttta acctgcgcga
     3421 gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa gccggatgag
     3481 cgggatcaac atcgacaacc gcatgatccg taaaggttct gtcgatgcca ttcgccgcca
     3541 tattgaggct aacggcggtc acttccctgc cgatgttgat caaaaagtcg atcaggttgc
     3601 gcgtcaagga gccacgctgc tggtggtggt ggaaggttct cgtgtgctgg gcgttattgc
     3661 gctgaaagat atcgtcaaag gcggtattaa agagcgcttc gcccagctgc gcaaatgggc
     3721 attaaaacgg tgatgattac cggcgataac cgtctgactg ccgccgcgat agctgcggaa
     3781 gcgggtgtcg atgattttct cgccgaagcg acaccggagg ccaagctggc attgatccgt
     3841 cagtatcagg cggaaggtcg tttggtagcg atgaccggcg acagcaccaa cgatgctacg
     3901 gcgctggcgc aggcagatgt cgcggtggcg atgaactccg gcacccaggc ggctaaagag
     3961 gcgggcaata tggtggatct cgactctaac ccgaccaagt tgatcgaggt ggtgcacatt
     4021 ggcaaacaga tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg
     4081 gcgaaatact tcgccattat tccggcggcg ttcgcggcaa catatccgca gttaaatgcg
     4141 ctgaacatca tgcgcctgca ttcgcccgac tccgccattc tcagtgcggt cattttcaac
     4201 gccttgatta tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt
     4261 accgcttctg ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg
     4321 ccgtttattg gtatcaaagt cattgattta ctgctaaccg tttgtagtct ggtgtgaggt
     4381 ttaccatgag tggattacgt ccggcattat cgacatttct ctttctgtta ttgattactg
     4441 gcggcgttta cccgctgctg accaccgcac tggggcaatg gtggtatccc tggcaggcca
     4501 acggttcgtt gattcgtgaa ggtgatacgg tgcacggttc ggcattaatc gggcaaaatt
     4561 ttaccggcaa cggctatttt catggtcgcc cgtcggcaac agcagaaatg ccctataatc
     4621 cacaggcttc tggcgggagc aatctggcag tcagtaaccc ggagctggat aaacagatag
     4681 ccgcacgcgt tgccgcatta cgggccgcta acccggacgc cagcacgaac gttccggttg
     4741 aactggtgac ggcgtcggca agcggactgg acaataacat caccccgcaa gcggcggcct
     4801 ggcagatccc acgcatcgca aaagcgcgta atctcagcgt tgaacagctc acgcagctga
     4861 tcgcaaaata cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg
     4921 tcgaactcaa tctggcactg gataagcttg atgaataacg aacccttacg tcccgacccc
     4981 gaccgtctgc tggaacaaac tgccgcgccg catcggggga agctgaaagt tttcttcagt
     5041 gcctgtgcag gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg
     5101 caagggctgg atattgtggt tggtgtggta gaaacccatg ggcggaaaga taccgccgcc
     5161 atgcttgaag ggctggctgt tctgcctctg aaacgccagg cgtaccgtgg gcggcatatc
     5221 agcgagtttg atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa
     5281 ctggcgcaca gtaatgcgcc aggttctcgt catcctaaac gctggcagga tatcgaagaa
     5341 ctgctggaag ctggcattga tgttttcaca accgtcaacg ttcagcatct ggaaagtctg
     5401 aatgatgtgg tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt
     5461 ttcgatgccg ccgacgacgt ggtgctggtg gacttgcccc cggacgatct gcgccagcgg
     5521 ctgaaagaag gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc
     5581 cgcaaaggca atctgatcgc cctgcgcgaa ctggcactgc gccgtactgc cgatcgcgtt
     5641 gatgagcaaa tgcgcgcctg gcgggggcat cctggcgaag agaaagtctg gcacacgcgc
     5701 gacgcgatcc ttttatgcat cggccataac accggcagcg aaaactggtc cgcgcggcgg
     5761 cgcggctgac gtcacggctg gggagcgtct ggcacgcggt gtatgttgaa acccctgccc
     5821 tgcatcgctt accggagaaa aaacgtcggg caattctcag cgccttacgt ctggcgcagg
     5881 agctgggcgc ggagacggca acactttctg atccagcgga agagaaagcg gtagtgcgtt
     5941 atgcccgtga acataatctc ggcaagatta ttctcggtcg cccagcctcg cgccgctggt
     6001 ggcgtcggga aacatttgct gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc
     6061 tggtcgcgct tgatgaacca cccgcccgca cgattaacaa cgcgccggat agccgctcat
     6121 ttaaagacaa gtggcgggta cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg
     6181 ttatcacctt aattgccatg cagtggttga tggcgtttga tgccgccaac ctggtgatgc
     6241 tgtatctgct tggcgtggtg gtggcgctat tttatggacg ctggccttcg gtggttgcca
     6301 ccgtcattaa tgtggtgagt ttcgatctct tttttatcgc cccacgcggc acgctcgccg
     6361 tctctgatgt gcaatatctg ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg
     6421 ggaaccttac tgctggcgtg cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca
     6481 cacggcactt atatgaaatg tcgaaagctc tggcggtggg ccgcagtccg caggacatcg
     6541 ccgccaccag cgaacaattt attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc
     6601 ccgatgacaa cggtaaattg cagccgttaa cacatccgca aggagttacg ccatgggacg
     6661 atgccatcgc gcagtggagt tttgataaag gcctgcctgc gggcgcgggc accgacacgt
     6721 tacccggtgt accgtaccag attttgccgc taaaaagcgg cgagaaaacc tacgggctgg
     6781 tggtggtgga gccggggaat ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg
     6841 agacttttac gctgttagtc gccaatgccc ttgagcggct gacgctaacc gccagcgaag
     6901 aacaggcgcg gatggcaagc gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt
     6961 cgcatgattt acgcacgccg cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg
     7021 atctggcaag cgaaggatca ccccacgccc gccaggccag cgagattcgt cagcatgtgc
     7081 tgaacactac ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct
     7141 ttaatttgaa gaaagagtgg ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc
     7201 cggaaccggg tttatcgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc
     7261 acgttgacgg gctactcttt gaacgggtgc tgattaatct gctggagaac gcggtgaaat
     7321 atgcgggtgc gcaggccgaa attggtatc
//
LOCUS       DAJVPP010000161         7109 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVPP010000161 DAJVPP010000000
VERSION     DAJVPP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.161"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..768
                     /gene="ydfJ"
                     /locus_tag="OQM34_003767"
     CDS             <1..768
                     /gene="ydfJ"
                     /locus_tag="OQM34_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCS1454024.1"
                     /translation="VMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFW
                     LATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMISSILGFMTIPFLGWLSD
                     KIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENIT
                     MAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGL
                     ISALKMLEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            857..2317
                     /locus_tag="OQM34_003768"
     CDS             857..2317
                     /locus_tag="OQM34_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1454025.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2353..2556)
                     /gene="ydfZ"
                     /locus_tag="OQM34_003769"
     CDS             complement(2353..2556)
                     /gene="ydfZ"
                     /locus_tag="OQM34_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1454026.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2734..3420)
                     /gene="ydfH"
                     /locus_tag="OQM34_003770"
     CDS             complement(2734..3420)
                     /gene="ydfH"
                     /locus_tag="OQM34_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCS1454027.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDADAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3509..4255)
                     /gene="ydfG"
                     /locus_tag="OQM34_003771"
     CDS             complement(3509..4255)
                     /gene="ydfG"
                     /locus_tag="OQM34_003771"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1454028.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQRHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4392..6437
                     /gene="dcp"
                     /locus_tag="OQM34_003772"
     CDS             4392..6437
                     /gene="dcp"
                     /locus_tag="OQM34_003772"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1454029.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6482..7000)
                     /locus_tag="OQM34_003773"
     CDS             complement(6482..7000)
                     /locus_tag="OQM34_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCS1454030.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVTAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1769 a         1679 c         1802 g         1859 t
ORIGIN      
        1 gtgatggtct ttgctatctg gttgcgtatg aatctgaaag aaagcccggt ctttgagaag
       61 gttaacgaca gcaaccaacc gacagcaaaa cctgcacctg ctggtagcat gttccagagc
      121 aaatccttct ggctggcaac agggctgcgt tttggtcagg cgggtaactc cggtttaatt
      181 cagactttcc ttgcaggcta tttagtacag acgttattgt ttaacaaagc aattccaaca
      241 gatgcattga tgatcagttc gattctcggc tttatgacca ttccgttcct tggttggtta
      301 tccgataaaa ttggtcgccg gatcccgtat attattatga atacctctgc gattgtgctg
      361 gcgtggccaa tgctttctat catcgtagat aaaagctatg ccccgagcac cattatggtt
      421 gcactgattg tgattcataa ctgtgcggtg ctgggattat ttgctctgga aaacattacc
      481 atggcagaaa tgttcggctg taaaaaccgc ttcacccgga tggctatttc aaaagaaatt
      541 ggtggtctta tcgcttccgg ttttggtcct atcctggcgg gtattttctg caccatgacg
      601 gaatcctggt atccgatcgc cattatgatc atggcatatt cagtgattgg tttaatctct
      661 gcgctgaaaa tgctagaggt gaaagaccgt gatttaagtg cgctggaaga cgctgcggaa
      721 gatcaaccgc gtgttgtaag agctgcgcaa ccttccagaa gtctgtaaac cctcaatccc
      781 ttctcttacc ggagagggga tttttattca tataacaaaa catatagctt gccatattta
      841 aatttaagga attgttatgg gaaataattt gttatcagca aaagcgacac tccctgttta
      901 tgatcgtaat aacctggccc caagaattgt tcatttaggc tttggtgcat ttcaccgtgc
      961 gcatcagggc gtgtatgccg atattcttgc tacagaacat ttcagtgact ggggatatta
     1021 tgaggtcaac ttaatcggcg gcgaacagca aattgccgat ttacaacagc aagataatct
     1081 ttataccgtt gcggaaatgt cggccgatgc gtggacggct cgcgtcgttg gcgtcgttaa
     1141 aaaagccttg cacgtacaga tggatggctt agaaaccgtg ttggctgcga tgtgtgaacc
     1201 gcaaatcgcg attgtctctc tgacaatcac cgaaaaaggg tatttccact ctccggcgac
     1261 cggacagtta atgctcgagc acccgatggt cgctgccgac gtgcaaaatc cccaccagcc
     1321 gaaaacagca acaggggtga ttgttgaggc gctggctcgc cgtaaagcgg caggacttcc
     1381 cgcatttacc gtcatgtcat gtgacaacat gccagaaaac ggtcatgtta tgcgtgacgt
     1441 tgtcacttcc tacgcgcaag ccgttgatgt aaaactggca caatggatcg aagataacgt
     1501 gactttccca tcaacaatgg tggaccgtat tgtgcccgca gtgacagagg atacgctggc
     1561 gaaaatcgaa caacttaccg gtgtgcgcga tcctgctggc gttgcctgtg aacctttccg
     1621 ccagtgggtt atcgaagata actttgtagc cggacgtccg gaatgggaaa aagcgggagc
     1681 cgaactggtt agtgatgtgc tgccttatga agagatgaag ttgcgcatgc tcaacggcag
     1741 tcattcattc ctggcatatc tgggttatct tgctggatat cagcacatta atgactgtat
     1801 ggaagatgaa cattatcgtc atgcggcgta tggcatgatg ttgcaggaac aagcgccgac
     1861 gctgaaagtg cagggcgttg atttgcaaga ttacgctaac cgattaattg cacgctatag
     1921 caacccggcg ttacgtcatc gaacctggca gattgcgatg gatggcagcc agaaattgcc
     1981 acagcggatg ttggattctg ttcgctggca tctggcgcat gacagcaagt tcgatctgct
     2041 ggcgctgggc gtcgcgggtt ggatgcgtta tgtcggtggt gttgatgaac agggaaatcc
     2101 gatagaaatc agtgacccac tgttacctgt tattcagaag gctgtacaaa gtagtgccga
     2161 agggaaagcg cgcgtccagt cattgctggc gattaaggca atctttggtg atgatttgcc
     2221 agacaatagc ttgtttactg caaaagtgac ggaagcgtac ttgtctttat tagcgcatgg
     2281 cgcgaaagcg accgtggcga aatattccgt gaagtaaaaa aagcaatgcc gccatcggcg
     2341 gcattcacac acttagttca tgccgagacg aatcaggtcc agaggtgcca gtttctcttc
     2401 acaaccttca acaactacgg tttttccgcg gcggatttgc tcagcggtca gaccttcagt
     2461 atcaatcgac aggatcttgc cagtgtgacc ggtgccgcta accataacac ggctgccagt
     2521 ggtgattgcg ttacggttac gatcgtaggt cgtcataggt gttttctcct ttctgattta
     2581 cagtcgaagc ccacgtaacg ggcgcaatat aaatactcct gtcgagtgaa atattttttg
     2641 tttttgatca agctcacatc ttttttgatg catgcatgag ccgtaaggcg gggggaaaag
     2701 cctctcatcc cggatgggat gagagtaaat gaattactct tcgctaaacc agtcgctgtt
     2761 ttcctggcgg atctggcgca cggattcgct gatttcctgc aaatgttgcg tcattgcacg
     2821 ttctaccgca tcggcatcac gtttttgcag ggcagagaaa atatcaagat gctggcgtaa
     2881 cagcatttct ggtggagaaa catggtcgaa actcatatag cgcacgcgat caacggtcgc
     2941 tttcaggttc tcaatggtat cccacgccag ttgacagtcg gcaatctgcg tcaggagttg
     3001 atggaagttg tcatcaagtt caaaaaaatc atccagttgc ttgcgctcaa tggcaatgcg
     3061 ttgctggtga agattttgtt ccagttggta gcactggctt tcggtaatca tgctcgccgc
     3121 ccgacgcgcc accgcgcact caatggcctg acggataaaa ctgccgttgc gcacctgggc
     3181 catggaaatt ttgttgacgt agctgccacg ctgcggacga atttgaatca ggccgttttc
     3241 cgccagttta ataaaggctt cacgaaccgg ctggcgtgac acattgaaac gaacagaaac
     3301 ttctttttcc gacaacggtg tgcctggtgc aatcaggcaa tggacaatgt cgcgacgaag
     3361 aatacgataa atctgctgat tgacaggctg tgtgggatta agttgcgttt cgacggtcat
     3421 tcgttcttgc ttgtgagtga gttaactgca tgagtctacc acttttttgt gacaagcaat
     3481 aacccggcag ttacgccggg tataaaaatt actgacggtg gacattcagt ccggcatagc
     3541 tttgggtaac cggcatcatt tccagggtat tgatattgac gtgagcaggc agcgttgaca
     3601 cccaccagac ggcttcgctg acatcttctg gcgtcaatgc aacggtattt tgataggttt
     3661 tttccgcttt accgtcatcg cctttaaagc ggacattgga aaactcggtg ccacccacca
     3721 gacccggttc gatgtcggtg acgcgcaccg ccgtaccatg cagatccgta cgcagattca
     3781 ggctaaactg acgaacaaac gctttcgtcg caccgtaaac gttaccaccg gcatacggcc
     3841 agctgcctgc cgttgagcca atgttaataa tatgaccatg attacgttca accatacccg
     3901 gtaagacggc gcgcgtcata tatactaggc ctttgttgtt ggtatcaatc atcgtttccc
     3961 agtcttcaac gctggcttta tgcgcaggct ccatgcccaa cgccaggccg gcattattta
     4021 ccaggatatc aatattgctc cactcggcag ggagcgatgc cagcatctct tcaatagcgg
     4081 cgcggttgcg aacgtccagt tgggcgatat acagattatc tcccagttcg tctgttaact
     4141 cctgcaaccg ctcctggcga cggccagtgg cgataacttt atgcctttgt tgaataaaac
     4201 gacgagtaat gcattcacca aaacctgccg ttgctccagt tactaaaacg atcatctcac
     4261 tgttcctcaa cgcttttgtg tgtcaataac atagcacgtg atgcttaaga gcgggtaata
     4321 ctggtttgca agattgcact ccgttatcag taagcctacg ctatggaaca cctcgcgttc
     4381 aggagaagaa aatgacaaca atgaatcctt tccttgtgca aagcacactg ccgtatctgg
     4441 ctcctcattt tgatcaaatt gccaatcatc actatcgccc ggcattcgat gagggaatgc
     4501 aacaaaagcg ggcagaaatt gctgccatcg cgcttaaccc gcaaacgcct gatttcaaca
     4561 atactattct ggcactggaa caaagcggag aattaattac ccgcgttacc agcgtctttt
     4621 ttgcgatgac tgcggcgcat accaatgatg aattacagcg tcttgatgaa cagttttccg
     4681 ctgaactggc ggaactggct aatgatatct atctgaacgg tgaattattc gcgcgggtag
     4741 atgctgtctg gcagcgccgt gaatccctgg ggcttgatag tgaatccatc cgcctggtgg
     4801 aggttattca tcaacgtttt gtccttgccg gagccaaact tgcacaagct gataaagcaa
     4861 aattaaaagt actgaataca gaagctgcga ccctgaccag ccagtttaac cagcggttac
     4921 ttgcagcaaa taaatccggc ggtctggttg tgaacgatat cgcgcagctg gcaggaatga
     4981 gtgagcaaga gattgcgctg gcggcagagg cggctcgcga gaaaggtctg gataacaaat
     5041 ggctgattcc gctgctgaat accacccaac aaccggcgct tgccgaaatg cgcgatcgtg
     5101 cgacgcgtga aaaactgttt attgcgggct ggacgcgagc ggaaaaaaat gatgccaatg
     5161 atacccgcgc tatcattcaa cgtctggtag agattcgcgc acagcaggcg aaactgcttg
     5221 gttttcctca ttatgccgca tggaaaatcg ccgatcagat ggcaaaaacg ccagaagcag
     5281 cactcaactt tatgcgggaa attgttccag cggcgcgtca acgtgcgagc gatgaattag
     5341 cctccataca ggcggttatc gataagcagc agggcgggtt tagcgcgcag ccgtgggact
     5401 gggcatttta tgcggaacag gtacggcggg agaaatttga tcttgatgag gcgcagctca
     5461 agccatattt tgaattaaac acggtgttaa atgaaggtgt attctggacc gcgaatcagc
     5521 tcttcggtat taagtttgtc gaacgttttg atattccggt ctaccatcct gacgttcgtg
     5581 tgtgggaaat ttttgatcat aatggcgtgg gactggcgtt attttacggt gatttcttcg
     5641 cccgtgattc aaaaagcggc ggtgcatgga tgggcaattt tgttgagcaa tcaacgctta
     5701 atgaaacgca tccggtaatt tataacgtct gcaattatca gaaacccgct gccggtgagc
     5761 ctgcgttgtt actctgggat gatgtaataa ccttattcca tgaatttggt catacgctgc
     5821 acggcctttt tgcccgccag cgttatgcca cgctttccgg caccaacacg ccgcgtgatt
     5881 ttgtcgaatt tccgtcgcaa atcaacgaac actgggcaac gcatccgcag gtattcgctc
     5941 gctacgcccg gcattatcag agcggggcag caatgcctga cgaactgcaa cagaaaatgc
     6001 gtaatgccag cctgttcaac aaagggtatg agatgagcga actgcttagc gccgcacttc
     6061 tcgatatgcg ctggcattgc ctggaagaaa acgaagcaat gcaggatgtc gatgattttg
     6121 aattgcgggc gctggtggcg gaaaatatgg atcttcctgc tataccgcca cgctatcgca
     6181 gcagttattt cgcccatatt tttggtggcg gatatgctgc aggttattac gcttatctgt
     6241 ggacgcaaat gttggccgat gatggttacc agtggtttgt tgagcagggc ggattaacgc
     6301 gtgaaaatgg gcagcgtttt cgcgaggcga tcctttccag aggtaacagc gaagatctgg
     6361 aacgcctgca tcgacaatgg cgtggtaagg ctcctcagat tatgccgatg ctgcaacatc
     6421 gtggcttgaa catataaaca ccaccaatgg ctggcctgga gatatcgcca ggccagcctg
     6481 gttatagcag taattgcagc agctgggcga gggcaaacct caccgctaaa gctaataccg
     6541 tttcgcagtc gccagcaaaa tgcataaccg cagtgtagtt ctggccttta atatgccacg
     6601 cgaaccagac ggtacccgct ggcgtaccat cttcaccgcc ctccggtccg ccgtagccgg
     6661 taatggcaat actgacatca gcatccgcac gctctatggc acctgttgcc atttctgctg
     6721 tcactttctc actcaccgca gaatatcgtt caagagactg ctggcttacg ctgaggattt
     6781 tcatctttgc ctgatcggtg aaagtaacaa agcctgcacc gtaaaatttg ggtgtatctt
     6841 cagctgcaca cagggcgcta gccaacttac cgccggtgca tgattcggcg gtagttaagc
     6901 gtaattgatc cgcgataagt ctttgactca acgcggatgt caggttttca atagtgtcgg
     6961 tatctgcgag ttgtacaatt ttatctttat taatgttcac tgttttttgt ccacgctatt
     7021 atatgtaaag gtgatttgct ttttctgtat gctcgtccaa tataataatt ttcaaacata
     7081 taaatccgat tattctgaaa acatgttta
//
LOCUS       DAJVPP010000162         6852 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVPP010000162 DAJVPP010000000
VERSION     DAJVPP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.162"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1235
                     /locus_tag="OQM34_003774"
     CDS             <1..1235
                     /locus_tag="OQM34_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCS1454031.1"
                     /translation="KKIRNIMIVVFLLSLLIPISPINAFFSSSYKVYSEAKEYNMSPH
                     EISDIKSKYTKEIVTNDSYSHEIINGNGKNILVIFIEGMSSLVISKELTPNLYEFRKK
                     SISFNNYYNHTAATFRGLRGQLTSSYQMTGGYHKDNSGLGQASKVEIEHKLNTYNYIT
                     KLPIILEENGYHTYFQASNSIDDPLSLMLSTLKFNHIYGREDYNKKSGELTDKESFDL
                     LFKKLNEAKQPFFYGVYTVGTHLGLDSPDVKFYDGRNEYLNHFHQLDHWFGDFISHFN
                     KTSLSKDTIIIITSDHASYPSEDYKKTFKTDNTFFVDQIPLIIWSNGVEFKDINAHGL
                     NSLSLTPTILDILGIKESHNMFIGESLFEKEKNKNELNCTSAIGDLYVKTCNGKMEIE
                     KRKEKTELIKQLQLFGDR"
     gene            complement(1304..2114)
                     /locus_tag="OQM34_003775"
                     /pseudo
     CDS             complement(1304..2114)
                     /locus_tag="OQM34_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009652796.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(2182..2418)
                     /locus_tag="OQM34_003776"
     CDS             complement(2182..2418)
                     /locus_tag="OQM34_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1454032.1"
                     /translation="MTEMERQLLSALESLQTSYEQQQQAWQDSYSSVQRMFETTSQAL
                     ERNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
     gene            3757..4037
                     /locus_tag="OQM34_003777"
                     /pseudo
     CDS             3757..4037
                     /locus_tag="OQM34_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4434..4802)
                     /locus_tag="OQM34_003778"
     CDS             complement(4434..4802)
                     /locus_tag="OQM34_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454033.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYG
                     LNISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAE
                     EESQRVVESFRDSLSRKKGF"
     gene            complement(5187..5336)
                     /locus_tag="OQM34_003779"
     CDS             complement(5187..5336)
                     /locus_tag="OQM34_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454034.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMVQVTEENLHAPVAFGPPV
                     GREAL"
     gene            complement(5581..6272)
                     /locus_tag="OQM34_003780"
                     /pseudo
     CDS             complement(5581..6272)
                     /locus_tag="OQM34_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1935 a         1541 c         1475 g         1901 t
ORIGIN      
        1 aaaaaaaaat aagaaatata atgattgttg tttttttatt atcattgtta ataccaatat
       61 ctccaataaa tgcttttttt tcttcatcat ataaagttta ttcggaagca aaagaataca
      121 atatgtcacc acatgaaata agtgatataa agtcaaaata cacaaaagaa atagtaacaa
      181 acgacagtta ctcacatgaa ataataaatg gaaacggaaa aaatatttta gtaatattta
      241 ttgaaggtat gtcttcattg gtaatatcta aggagctaac cccgaattta tatgaattta
      301 gaaaaaaatc aatatcattt aacaactatt acaaccatac cgcagctaca tttagaggtt
      361 taagaggaca gttaacctca tcatatcaaa tgaccggtgg gtaccataaa gataattctg
      421 gccttggcca agcatctaaa gtagagatcg aacataaact aaatacttac aattacataa
      481 caaaattacc aatcatactt gaggaaaatg gttaccatac atattttcaa gcatctaatt
      541 caatagatga cccattgagt ttaatgctat caactttaaa gtttaatcat atatacggaa
      601 gggaggacta taacaaaaag tcaggtgaac tcacagacaa agagtctttc gatttattat
      661 ttaaaaaatt aaatgaggct aaacaacctt tcttctatgg tgtttatact gtaggtacac
      721 accttggctt agatagccct gatgtaaagt tttatgatgg gagaaatgaa tatttaaatc
      781 actttcatca attagaccat tggtttggtg attttatatc tcattttaat aagacaagct
      841 tatcgaaaga tacaattatc ataatcacaa gtgaccatgc aagttaccca tcagaagatt
      901 acaaaaaaac attcaaaact gataatactt tttttgttga tcagattcca ttaataatat
      961 ggagtaatgg tgttgagttc aaagacatta atgcacacgg tttaaattca ctatctttaa
     1021 cgccaacaat actagatatt cttgggatca aggagtcaca caacatgttc ataggtgagt
     1081 ctctatttga aaaagaaaaa aataaaaatg aactcaactg tacatctgca ataggtgacc
     1141 tgtatgtgaa aacgtgtaat ggaaaaatgg aaatcgagaa aagaaaagaa aaaacagaat
     1201 taataaaaca attgcaactt tttggtgata gataattaaa aggcggtttt accccatgtt
     1261 gattattagt ccacgtgggg ttagtttaaa atgatagtaa aacttacttt ttttcattct
     1321 tgagccgcct gtagaatgtg gctcgactaa cacctaaaga ctcccattct ttttttaaag
     1381 ttataggaat taatattaaa actaccttta ggccgtcctc ctgaattgct tagctttcct
     1441 cctttacccc ctctctttct ttgaatttct gaacggtgcg tattttttac atactcaata
     1501 aaacgatcct ctgaaaaatt attcgctgtc cattttgaga tgctttttga tattccaaga
     1561 acttcatttt cacttaatgg tgatacgaat tgtaaattgt aggcacaggc tcgttcatag
     1621 caggcttgta accactgctc atactcaggc cacccctgac ggatggcacg atatgcccac
     1681 ttgcgggttt tgtcgaacag agtgcagtta cggcctaaac cataatcggc ggcaattttc
     1741 cagtgactgt ggttcggatt cttgcaaatt agcccagaat atcccgtgtc agctcccaat
     1801 tttctacgta gcgcattctc tacggcagct gcgtagcgta acggtttcat ttttccgtca
     1861 ggtgctgtgc gtatagaggt ttccagcgca taaagcaggt gcgcgtgtcc gttttcgggg
     1921 ttggtgatgg tcagcgtagg tgcgggagcg ttgcggtcgc tccagtcaat ggctgcgccg
     1981 atgcggtcaa catcaaatcc cagccagaac atggcgtgag gctgattgaa ctggatgtat
     2041 ttcgccagaa tagcgcgttc tttccctgcg atgcgcacac caaagtgcaa atcatccgag
     2101 aagtacggct tgtgtgatac atggactgac ctcgatagcg ggttgcttgc ttctcctgct
     2161 gtaactgccg ttcctgctgc tttacgagcg tgttaatcgg ctcagatcgc tgctcaagcg
     2221 ttcgacctgt tcgtgcaagg cgtttacctg attgctcaag ttcagacata ccctgtcgtt
     2281 gcgctccagt gcctgtgagg tagtctcgaa catgcgttgc acgctgctgt aactgtcctg
     2341 ccacgcctgt tgctgttgtt cgtaacttgt ttgtaagctt tctaaggcgc tcagaagctg
     2401 cctttccatc tcggtcatgg ttttgcctgt cctcagtgat aaaaacgtgc tgtggctttt
     2461 ctgtgggctc tgacggggtt atttcgaggt gtacttcatc acggtgtttc cggctgtcga
     2521 tgtataggtc tcggtgccat gcgctttctt cggcagtacc accagatagg tgttcttctg
     2581 cttgtcgtcc ttgtaaatcg ctacccctgc gccgctctgg gctttcaggc tctccagttg
     2641 ctggctgtac ggtcacaccg ttcaagcaga cgctgatgct cagtctcggt catgcggatt
     2701 ttgatttctt tcgttcgctt ttccataggc tccgctgaga aaaaattcgc accactgtga
     2761 gaaagtgctt catgtggcag gacaaaaaag gcggcaccag tgcgtcagca gaatatgtga
     2821 tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     2881 cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaaaatactt
     2941 aacagggaag cgatagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
     3001 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga cttaaagata
     3061 ccaggcgttt ccccctggtg gctccctcgt gcgctctcct gttcctgccg ttcggtttac
     3121 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     3181 ggaaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct
     3241 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacacgac aaagcgccac
     3301 tggcagcagc cactggtaac aggattagca gagcgagtta atgtaggtgg tgctatagag
     3361 ttcttgaagt ggtggcctaa ctacggctac actggaagga cagtatttgg tatctgcgct
     3421 ctgctgaagc cagttaccag gttaagcagt tccccaactg acttaacctt cgaaaaacca
     3481 cctccccagg tggttttttc gttttcagag caagagatta cgcgtagaaa aaaaggaact
     3541 caagaagatc ctttctatgc tcaaccaccg catggctcat agccgcgctg gccgttgccg
     3601 gtcagctcca gaatcctcga ccaatatgga accttgcccc tcccaaaatt caactaaaaa
     3661 accctcattt cctgccgata aaatgggtat ctcttaacta tgcctaaata aggataattt
     3721 tgaaaaaaaa tattcatcct atcggcagcg ctgatcatgg caggatacgc aacaaagcaa
     3781 tatccacagg ccgcgaacgt caccggtcca gaatcttccg caatggattg caaagcggta
     3841 gctctggaaa tcgccaaaac gcgcagtgta caacaagaaa ttgaggaaac cggcgaattt
     3901 gacgggcgca ccgtattagg tttcctgggt gattttggga taggaaatgg catggcaaaa
     3961 ggcgaagccc gcaaaaagca gctaaccggc taaatcagct ggaagccctg aaggctgcaa
     4021 agtgtgcaga tcaataaggt acaaaaaagc cctgcggggc ttttttgtac ctgccaatca
     4081 gttggattta ccataacatc cgttgcgtgc accgcctgag aggctctgag agcgtttctg
     4141 acgggaaacc ggcaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct
     4201 ctgtttttac ggcatgcatg agtatgcacg caagtgcata gcgcgaaaat tcaccccatt
     4261 cagccagggt aagtcctgtt tccggcattc ttcccatccg aaaacggtga acggggacgc
     4321 aatttattgc gtggccgtga ggcattgacc aagccgtaag gcacggtagg taattgcagg
     4381 tatccgacaa tttcgagtag aggatccact atttcactac ttagaattca aaatcagaat
     4441 cctttcttcc tgctaaggct atctctgaat gactcaacca ctctttgaga ttcttcttct
     4501 gctcgcatga agtcattccc ggacagaaaa aaaccattgt caggcccact ggcttcagag
     4561 aaattcgttt ctgcagcaga cacagaatcc acatcagcaa gagacggttc atctgtactt
     4621 tttctcaatc tgtaaagagc cttatcaaac gcaaccagtg agatattcag gccgtaacgt
     4681 tcattcagca cagtcacaat ctcttcccta cggataccat ttttcttcca ggtttctata
     4741 tcgttcttgg aatccctgac agccgcaatg gctgttcttg gaccagggtt aaaattcgcc
     4801 atataccgcc cataatgtct taaatttgtc tcataatagt ccaaaaataa gattgaaacg
     4861 caaaaaaatg tcttacaatt gtcttattgt tgtcctttcc ggttcatgta tccatgaacc
     4921 gcctgatgtg gaacgctttt tatcgatctc atgcgcagcc aaagatagat tcattcttta
     4981 aaagtcaggt tacattatcc ctgaaatggt catacaccat acgcaaccgc gattttcatc
     5041 gcaattgcat gaattaaaag gtaaaaactg aagtccccgt ttatttatgt aatacgcaac
     5101 gtattacaac cacctgtaat tacatgaaaa taaagaaata agatgtaata ccggcgtatt
     5161 accacgtacg cgggtaagcg aaaaagctat aacgcttccc ggccgacagg cggcccaaaa
     5221 gccaccgggg cgtgcaggtt ctcttcagta acctgcacca ttaaatcaaa aaggtcatat
     5281 tcgccgggaa gctgcgtatc agtcgaatcc agttgttcat catgttcaga tggcatgggg
     5341 tgatatccag tagctgagag gtggttagcg ggtttcgggg cgcagcccta aaccagtcac
     5401 gtagcgctag cggagtgtat actgacttag gcggtagtgt agtatgacag attctgtggt
     5461 tacggcaaag taacagccct gtctaggaga gggctgttat ctgatatagg cttgtttttt
     5521 tctcaccgtt tttgcgagcg ctttttcctg ccaagaggta ggtagggtaa tgaatccaac
     5581 ttactgatag tgttttatgt ttagataatg cccgatgact ttgtcatgca gctccaccga
     5641 ttttgagaac gacagtgact tccgtcccgg ccttaccaga tgctgcctca gattatgccg
     5701 ctcaattcgc tgcgtatatc gcttgctgat aacgtgcagc ttttccttca ggcgggattc
     5761 ataaagcggc catccgtccg tcatcaatac caccacgtca aaggccgata gcaggctcag
     5821 aagacgctcc agcgtgaaca ttgtgcgttc accaaaaacg tgggccacga cagtcctgcg
     5881 taccctgtca tacgcgtaaa acagccagtg ctggcgtgat ttagccccga cgtagctcca
     5941 ctgttcgtcc atttccgcgc agacgatgac gtccctgccc ggctgtatgc gcgaggttac
     6001 cgactgcggc ctgagttttt taaatggcgg aaaatcgtgt tgaggccaac gccaataaag
     6061 cgggcggttg cccggcatcc gacgccattc atggccatat caatgatttt ctggtgtgta
     6121 ccgggttgag aggcggtgta agtgaactgt agctgccatg ttttacggca gtgagagcag
     6181 agatagcgct gatgtccggc agtactttta ccgttacgca caacgccgtc agtagcggaa
     6241 caggagggac agctgatgga aatggaagcc accggagcac cttaaaagca ccgtcatcca
     6301 ctaaatcagt aagttggaag catcaccgca ctagcagaca gttaattaaa aaagtataat
     6361 ccctctccgg atttgttgaa taatggagaa ttatgctgtt ttctatgtct tatttagtca
     6421 aatgcgctta tcaatattgt gataagctcc cgagtctaaa cttaacttac tgattttagg
     6481 ttgaggaagg ccggcatttt tgtagatcca cagcataata tctatttatt caacaacacc
     6541 tcccccaaaa aaaaaggttt ttataaatgt ttttaatttc aattattcaa atgttctcgg
     6601 ttatgattat aatgcttaat ataaaaataa aagatgcatt tagagcaatt attctgcttt
     6661 tacttctatt actatccatg gttatatcta ttgaaataag cagcattata tcaataggtg
     6721 agtacatacc cacacttgca ctagcaaata tatcagagac aaatagtgtt ggctggaaaa
     6781 taaaactact accttttctt gtgttcttgt catttatgat tgcatcattg attttgaaaa
     6841 gattgataaa ag
//
LOCUS       DAJVPP010000163         6447 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVPP010000163 DAJVPP010000000
VERSION     DAJVPP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.163"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            469..1116
                     /gene="fucA"
                     /locus_tag="OQM34_003781"
     CDS             469..1116
                     /gene="fucA"
                     /locus_tag="OQM34_003781"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1454035.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            1144..2292
                     /gene="fucO"
                     /locus_tag="OQM34_003782"
     CDS             1144..2292
                     /gene="fucO"
                     /locus_tag="OQM34_003782"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCS1454036.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2345..3100)
                     /gene="xni"
                     /locus_tag="OQM34_003783"
     CDS             complement(2345..3100)
                     /gene="xni"
                     /locus_tag="OQM34_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1454037.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3211..4578)
                     /gene="sdaB"
                     /locus_tag="OQM34_003784"
     CDS             complement(3211..4578)
                     /gene="sdaB"
                     /locus_tag="OQM34_003784"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1454038.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4636..5925)
                     /gene="sdaC"
                     /locus_tag="OQM34_003785"
     CDS             complement(4636..5925)
                     /gene="sdaC"
                     /locus_tag="OQM34_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1454039.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPV
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1667 a         1544 c         1695 g         1541 t
ORIGIN      
        1 catatcggtg aatatttgcc tgtctgaaat gaactcatgg attcattttc aaataaaaaa
       61 cgctctgtaa tatgacggcg gtcacactta ttagctggaa ggtgacttta tgtgactacc
      121 atcactttaa taatcgaaat ttcatgcccg tttcagaaaa taaatcataa aaaacacaaa
      181 ccagatgacc gtattaattg tttgtgccga aaacgggcat ttctggcgag gggaaatgga
      241 aaaatgaccg ttttgataaa tatttttaag agcggtcatt tttaacgggg ttataaaaag
      301 agaacttgca gttatttact gtgattagtt gagctaggtc acaaaatata agcttgtgaa
      361 tttctttctt gttaatcagg aagaagatgg tgggtaatta aacggctaat tcaatagtgt
      421 gaaaggaaca acattattgc cctgttttga atcagagaga gatagctaat ggaacgaaat
      481 aaacttgctc gtcagattat tgacacttgc ctggaaatga cccgcctggg actgaaccag
      541 gggacagcgg ggaacgtcag tgtacgttat caggatggga tgctgattac gcctacaggc
      601 attccatatg aaaaactgac ggagtcgcat attgtcttta ttgatggcaa cggtaaacat
      661 gaggaaggaa agctcccctc aagcgaatgg cgtttccata tggcagccta tcaaagcaga
      721 ccggatgcca acgcggttgt tcacaatcat gccgttcatt gcacggcagt ttccattctt
      781 aaccgatcga tccccgctat tcactacatg attgcggcgg ctggcggtaa ttctattcct
      841 tgcgcgcctt atgcgacctt tggaacacgc gaactttctg aacatgttgc gctggctctc
      901 aaaaatcgta aggcaacttt gttacaacat catgggctta tcgcttgtga ggtgaatctg
      961 gaaaaagcgt tatggctggc gcatgaagtt gaagtgctgg cgcaacttta cctgacgacc
     1021 ctggcgatta cggacccggt gccagtgctg agcgatgaag agattgccgt agtgctggag
     1081 aaattcaaaa cctatgggtt acggattgaa gagtaatttc gtaaagcaac aaggagaagg
     1141 atgatggcta acagaatgat tctgaacgaa acggcatggt ttggtcgggg tgctgttggg
     1201 gctttaaccg atgaggtgaa acgccgtggt tatcagaagg cgctgatcgt caccgataaa
     1261 acgctggtgc aatgcggcgt ggtggcgaaa gtgaccgata agatggatgc tgcagggctg
     1321 gcatgggcga tttacgacgg cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg
     1381 ctcggtgtat tccagaatag cggcgcggat tacctgatcg ctattggtgg tggttctcca
     1441 caggatactt gtaaagcaat tggcattatc agcaacaacc cggagtttgc cgatgtgcgt
     1501 agcctggaag ggctttcccc gaccaataaa cccagtgtac cgattctggc aatccccacc
     1561 acagcaggta ctgcggcaga agtgaccatt aactacgtga tcactgacga agaaaaacgg
     1621 cgcaagtttg tttgcgttga tccgcatgat atcccgcagg tggcgtttat tgatgctgac
     1681 atgatggatg gtatgcctcc ggcgctgaaa gccgcgacgg gtgttgatgc gctcactcat
     1741 gctattgagg ggtatattac ccgtggcgcg tgggcactaa ccgatgcact gcacattaaa
     1801 gcgattgaaa tcattgctgg ggcgctgcga ggatcggttg ctggtgataa ggatgccgga
     1861 gaagaaatgg cgctcgggca gtatgttgcg ggtatgggct tctcgaatgt tgggttaggg
     1921 ttggtgcatg gtatggcgca tccactgggc gcgttttata acactccaca cggtgttgcg
     1981 aacgccatcc tgttaccgca tgtcatgcgc tataacgctg actttaccgg tgagaagtac
     2041 cgcgatatcg cgcgcgttat gggcgtgaaa gtggaaggta tgagcctgga aggggcgcgt
     2101 aatgccgctg ttgaagcggt gtttgccctc aaccgtgatg tcggtattcc gccacatttg
     2161 cgtgatgttg gtgtacgcaa ggaagacatt tcggcactgg ctcaggcggc actggatgat
     2221 gtttgtaccg gtggcaaccc gcgtgaagca acgcttgagg atattgtaga gctttaccat
     2281 accgcctggt aaatgcgctg atgtgataat gccggatacg atttgcggcg tatccggctc
     2341 gccgttaccg taccaaccgc aattgctgta aattgccgtc gatatgcaaa tcggtttgta
     2401 agcgggcaat atcgcggcac agaaacgcca tctctttatg ggtttctaat tttttgcgcc
     2461 acttttcggc aaccgcatcc agattctcat atatcccttc cagactctga aactcgacca
     2521 gcagctgcgt ggcgcttttt ggtccgattc ccgcaacacc cggtaccttt gaactgctga
     2581 tccccgcaag tccccagtaa tcgggcaact gctgcggttg aacgccaaat tctttatcga
     2641 taaatggcgc atccagccaa cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa
     2701 gtaactgaca gtagccttta tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca
     2761 ctttgaccgc cagtgtggcg gctaagtcat cggcttcgtt gccgctggct gaccagcacg
     2821 ggacgccgcg ttgctcaaag gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg
     2881 gcattggcgg tcgatccgct ttgtaatctg gtaaacgctg atgacgccag ccgctactgc
     2941 ggttttcatc atcaaaaacg gcgaccgcgt gggttggctg gctgtgcata atgagctgat
     3001 cgagcgcatg ctggcaggtt tcgacacagg gcgaaccctg aacggcatga atgcgacgga
     3061 taagattcag tgcatcgaca ataagcaaat gaacagccac gataaccccc ttaaagatca
     3121 ggcgataagg gtaacattcg atgccggaac aggctatggc tggttgagaa aacaggaaga
     3181 ggcttcgcaa aacgaggcct ctgggagaga ttaatcgcag gcaacgatct tcatcgccag
     3241 gccgccgcga gaggtttcgc ggtacttggc gttcatgtct ttacctgttt cgtacatggt
     3301 ttcgataact ttatcgaggc agacgcgcgg ttcgctggta cggcgcagtg ccatacgcgc
     3361 ggcgttcacc gctttcactg ctgcaatggc gttacgctca atgcacggta cctgaacctg
     3421 ccccgcgacc gggtcacaag tcagaccgag gttgtgctcc atcgcgattt ccgctgcgat
     3481 gcaaacctgc gccgggcttg cgcctaacag ttctgccaga ccagctgccg ccatagagca
     3541 cgccacgcca acttcaccct ggcaacccac ttcggcacca gaaatcgacg cgttcatctt
     3601 ataaagagaa ccaatggcgc tggctaccag caggtaacga gccagtgagt tagcgttcac
     3661 ttcgcggata aacttatcgt agtacgccag cacagccggg ataatcccgc acgcaccgtt
     3721 agtcggcgca gtcaccacgc gaccgccagc agcgttctct tcgttcactg ccagtgcaaa
     3781 catgttgatc cagtcaacaa ccgccatcgg gtcagtggtg gttttatcct ggctgaccag
     3841 catccggcgt agtgccgcag cacggcgtgg aacgcgcagt ttgccaggca acacgccttc
     3901 ggtggaaata ccgcgctcaa taccgccacg catgacttcc cagacgttcg ccaggtgctg
     3961 ttccagctct tctttactgt gcagcgccag ctcgtttttc atcatcaggc cagagagtga
     4021 cagcccggtt tcctgacaat gtttttgcag atcggctgct gaactgtacg gataaggaac
     4081 ttcaaccggt gcgctatcct gctggccaaa atgctcttca tcaacgataa agccaccgcc
     4141 aatagagtag taagtctggc tgtaaacgac tttatcgccc gccagcgcgg taatgcgcat
     4201 accgttttca tgcagagaaa ggttgtctgc atggaagttc atgcactgat caaccgggaa
     4261 ctccacttca tgctgaccgt ttgccagcat caggcgacca tgagtattca catcctgaat
     4321 aaaaccgggg atggaatcga tatccacggt atccggcagg ttacccgcca ggcccataat
     4381 aatggcgata tctgtgtggt ggcctttacc agtcagagag agcgagccgt acacgtcaac
     4441 caccacgcgg gtcacgtctt taagcaggtt acgggcaatc agatcgtcgg taaattgttt
     4501 acccgctttc attggtccaa cggtatgaga actggaaggg ccaatgccga ttttgaaaat
     4561 atcgaatacg ctaatcatag gaaatacatc gcgttaaaac ggaggaagcg ccgcccgaaa
     4621 gcggcgcgaa aggacttagc tgaacagaga gtagaagatt gcggagattg caatcagacc
     4681 catcacgaca acgaatacgt tgctgatgtg accgctgtac ttacgcattg ccggtacttt
     4741 ctgaattgcg tacatcggca tcaggaacag gatcatcgcg atgattgggc cgcccagggt
     4801 ttcaatcata cccaggatgc tcgggttcag ggtggcaaca atccaggtcg ttaccagcat
     4861 gaacagcgca gtgatacggt tcagcttgtt gatttcgata gacttacctt taccacgcag
     4921 agatttaatc accataccgt tgaagccttc acgtgcgccc aggtagtggc cgaggaagga
     4981 tttggtgata gcgataatcg cgataatcgg agccatccac gcgataaccg gtgcgttaaa
     5041 gtggttagcc aggtaagaca gaatcgagat gttctgctct ttagccgcag ccaggtctac
     5101 cggagtcagg ctcagtacgc agctgaagac gaagaacatt acggtcagca ccatcatgat
     5161 gtgtgcgaat gccaggatct tcgagcattt ctgttctgcc atatcgccgt actcttcacg
     5221 cttcgcaacg gcgaaagaag agatgatcgg agagtggttg aacgagaaca ccattaccgg
     5281 aattgccagc cacagggtca tccacagacc gtttccggtt gcagatgcag tgtccagaga
     5341 cagcgtttcc agtgctgcgc cgttccactg cgggatcagg tacagagcca gcagcatcag
     5401 tacgccaaca aacgggaata ccagaatact catcgcttta acgatcatct gctcaccaaa
     5461 gcgaacgatg gtcatcatac ccacgatcag gatcagcgac agaatcgcac gcggcggtgg
     5521 cgtcataccc agctggtgag acatgaagct ttcaacggta ttggtgattg ccacgctata
     5581 aaccagcagg atcgggtaga tagcgaagaa gtagagcagg gtaatcagtt tacctgcgcc
     5641 aataccaaag tgttcttcta caacctcggt gatgtcttcg cccgggtttt taccagacag
     5701 tacgaagcga gtcaggccgc ggtgagcaaa aaacgtcatc gggaacgcaa ggatagccat
     5761 gatgatcagc gggatcatac cgccaacacc ggcgttgatt ggcaggaaca gcacgcccgc
     5821 gccgattgcc gtgccgtaaa ggcccagcat ccacatggtg tctgtcttgc gccaggcact
     5881 acgagagtct ttcgacgcaa tcgtgctggt ttgagtcgtt tccatctatt tctcctggag
     5941 gaatgcaaat aattcagctt ttagccagtt cagccaggga aggtgcctga ccgtaaaaat
     6001 gaaatctgtc tatcggcgat ggcttaatca tttaccgccg taataatttg agggcggaaa
     6061 gatacacaca agttatgtat ctatcagtga tcttgatccc aaatacaata atctgcttgg
     6121 aagatagatt atcccaacgc tttactctgt aaatcaaaag taaaagagaa tttaagggca
     6181 atatttgccc attaaacgac atctgacttc ttccggggcg cgcaatatat gtaggttgaa
     6241 ctgaaaaaat caatactttt agttaatcat ggcgataatt tctttttcat agcgataaaa
     6301 tacggtttat gaaaataggg aaacgtttgt gttgcaacat aaattgtatg acatattaac
     6361 tgtgatgata tttgtcgcgt gagggtggta agaaacattc atataacaaa tgaaatgagt
     6421 tgatgagtaa gtgaccaaaa acgagaa
//
LOCUS       DAJVPP010000164         6385 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVPP010000164 DAJVPP010000000
VERSION     DAJVPP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.164"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            57..530
                     /locus_tag="OQM34_003786"
     CDS             57..530
                     /locus_tag="OQM34_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1454040.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
     gene            619..1484
                     /locus_tag="OQM34_003787"
     CDS             join(619..982,982..1484)
                     /locus_tag="OQM34_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCS1454041.1"
                     /translation="MVHKSDSDELSALRAENARIIKPLLPPEPATPRAGRPWAEHRKI
                     INGMFWVLCSSAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFID
                     WSATALDGSNIRALKCAAGGSKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIV
                     LSPGQAHESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAV
                     IPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKL
                     GCIRLFYQRLRN"
     gene            1743..1927
                     /locus_tag="OQM34_003788"
                     /pseudo
     CDS             1743..1927
                     /locus_tag="OQM34_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            2070..2291
                     /locus_tag="OQM34_003789"
                     /pseudo
     CDS             2070..2291
                     /locus_tag="OQM34_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2312..2374)
                     /locus_tag="OQM34_003790"
                     /pseudo
     CDS             complement(2312..2374)
                     /locus_tag="OQM34_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            2824..4236
                     /gene="ospC1"
                     /locus_tag="OQM34_003791"
     CDS             2824..4236
                     /gene="ospC1"
                     /locus_tag="OQM34_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCS1454042.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(4340..4489)
                     /locus_tag="OQM34_003792"
                     /pseudo
     CDS             complement(4340..4489)
                     /locus_tag="OQM34_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4565..6262
                     /gene="ospD3"
                     /locus_tag="OQM34_003793"
     CDS             4565..6262
                     /gene="ospD3"
                     /locus_tag="OQM34_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCS1454043.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLIPDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         2125 a         1216 c         1279 g         1765 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggggg agatgaaccc tatgcaatgt
       61 cctggttaat atctcagtgt gctcatcagt gcactgataa taaaaagacg gagacagatg
      121 ccatttacga taaagttcgg agttcttatt tgctttcatg tattctgaaa aaaaacaaaa
      181 atgttgggct tattcttcat gcaccatcat ttgtttctgt gtctgaaaaa atcgccagaa
      241 ttgtgatggc aaattactcc cgtaactgga gtaattccga gttggccagt gcggttttaa
      301 tgagtgaatc ttctttaaaa agaagaatgt acaaagaggt cggttctata agtacatttg
      361 tacataaaat aaagcttaca gaagctatca ggaaattacg aaggacaaat actcccattt
      421 ctgtcatatc atctgagtta ggttacagtt caccatcgta tttttctaaa gtcttcttta
      481 aatatttaaa gacataccct caaaacataa ggaagaaaaa tggtcggtaa tgtcaccaat
      541 taggaggact tgcagagccg cttttttacg atactgagat caaacagaca taatctatgc
      601 ggagatttac agcagggcat ggttcacaaa tctgacagcg atgaattatc ggcactgcgg
      661 gcagaaaatg ctcgaatcat caagcccctg ttacctcctg aacccgccac accacgggcc
      721 ggacgcccat gggctgagca ccgtaaaatc atcaatggca tgttctgggt gttatgttcc
      781 agtgcaccat ggcgcgattt acccgaacga tatggggcat ggaaaactgt ttataaccgc
      841 tttaaccgat ggtcaaagtc aggcgtgatt aatattattt tcaacaggtt gctttcgcta
      901 cttgatgcaa acggctttat tgactggtct gccaccgcgc tggatggcag taatatccgg
      961 gcgctgaaat gtgccgccgg tgctcaaaaa aacatcccga tatcgacgga gataatgggc
     1021 cgggtcgctc tcgcggcggt tttggcacca aaatccatct ggcaacagac ggaagtggcc
     1081 tcccgttaaa tatcgtgcta agccccggac aagctcatga aagccagttc gcgcaacgac
     1141 ttctggacgg gattggcgtt cagcgtcaga acggcagcat gaaacgccgt ggacatgcgg
     1201 tactggctga taaagcgtat tcagggcgcg cattgcgcaa cgagctgaaa aataacggta
     1261 taaaggcagt aatcccccga aagtcaaatg agaaaatggc atcggatgga cgtgcacagc
     1321 ttgatcgtga tgcgtactgc aaccgtaatg tcgttgaacg gtgctttggg cggctgaaag
     1381 aatatcgccg catcgccacg cgttacgaca aaacggcgag aaattacctg gcgatggtga
     1441 aactgggctg catccgactc ttttatcaac gcttacgtaa ttaagggaca cagcctagtc
     1501 tccaggattc ccggggcggt tcaatcaaag cgcagaactt ctgcgtcttc ttcgtcaaaa
     1561 aaatcatctt cgtgaagctt cacgacatag cggggaagtt tgcttctttg agaggcgggt
     1621 ttacgtttac gggatttagc tgaacgggcc atataaccac ctgaaagaca atgacattgc
     1681 ctgtttttat aacggtaatt gcagaccatg acaaaccgca gccgtcaggc tgcctactcg
     1741 agtcacaagg ctctttgtgt ccgatatgcc ggcagcgcat tacccatgaa actggctgga
     1801 acgtacacca taaggtgaag aaagttatgg gggcggtgaa gaattatcta accttgtctt
     1861 gttacatcca aattgccatc gacagttaca cagttgtgaa gccggctctc tcaaaaaggg
     1921 cttataaagg cttgagcggt gtaccgggaa accggtatgc accgttctta ggggagggca
     1981 gtccagcaat gaactgtcct tacccgacaa acatccaaaa cgaacggaac gtgctggaat
     2041 ccgcatataa cccgttgtaa agtgaggaaa tggacacctc acttgctcat gagaacgccc
     2101 gcctgcgggc actgttgcag acgcaacagg acactatcca ccagatggct gaatacaacc
     2161 gcctgctctc acagcggatg gcggcttatg cttccgaaat caaccgactg aaggcgctgg
     2221 ttgcgaaact gcagcgtatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg
     2281 aacggcagat tccattttca cgggcgatct acgccacaca ggctctgggt tgcagtgact
     2341 gctccattaa ggcgattttg aactcataag ggaagtaaca aaaaaactgt aaaagtacaa
     2401 taattatata tggccaaact aacctctatt aaaaaacatc agtttttcac acttagttga
     2461 gcttcatgaa aaaatcatcg aggatttcat tgcagaatac acattccact aactggaagc
     2521 actccataag aggaatcaga gaaagtatcg tttttttata gtaaaattca ttgctgttca
     2581 ataaaattga tatagttatt aaattatgaa aataatttca caaaaaagat taacttgagt
     2641 tcttaagcat cggattttag atattttgaa atatctggca tcacataaca tcaaaagtat
     2701 ataataccct tatctaatta catgcagtat attgtatata tactcaaaaa ttgtaattca
     2761 taccctgcta tatcttgtag ggaaagatat taattaaaac tgttttcata taaggttcat
     2821 tttatgaata tatcagaaac actgaactca gcaaataccc aatgcaatat agattctatg
     2881 gataacagat tacatacatt gtttccaaaa gtgacatcag tgcgaaacgc tgcacaacaa
     2941 actatgccag atgaaaaaaa tttaaaagat agtgcaaata ttattaaaga tttctttagg
     3001 aaaactatag cagcacagag ttatagtaga atgttctctc aaggctctaa ctttaaatct
     3061 ttaaatatag caattgatgc accatcagac gctaaagcct catttaaggc tattgagcac
     3121 cttgacagat tatcgaagca ttatatatct gaaataaggg aaaaacttca tcctctttct
     3181 gcagaggaac tcaatttgct ttcgctaatt attaattctg atttaatctt cagacatcaa
     3241 agtaattctg atttgtctga taaaatttta aacattaagt cattcaataa aattcagtct
     3301 gaaggaatat gcacaaaacg aaacacatac gctgatgata taaaaaaaat agctaatcat
     3361 gactttgtgt tttttggcgt tgaaatctct aaccatcaga aaaaacaccc cctgaataca
     3421 aaacatcaca ctgttgattt tggtgcaaat gcgtatatca ttgatcatga ctctccatat
     3481 ggatatatga cattaaccga tcactttgat aatgctattc cacctgtttt ttaccatgag
     3541 caccaatcat ttttagataa attttcagag gttaataaag aagttagtcg atacgtacat
     3601 ggaagtaaag gaattataga tgtaccaata ttcaatacta aagatatgaa gttagggctc
     3661 ggattatacc tgattgactt tattagaaaa agtgaagacc aaagcttcaa ggagttttgc
     3721 tatggaaaaa atcttgcccc tgtggatctg gatagaatca taaactttgt ttttcagcca
     3781 gagtaccata tacctaggat ggtaagtaca gaaaacttca aaaaagttaa gattagagaa
     3841 atatccttag aggaggctgt tacagcatct aattacgaag aaattaacaa gcaggtcact
     3901 aacaaaaaaa ttgctctcca ggctcttttt ctttcgatta ctaatcaaaa agaggatgtc
     3961 gccttatata tattatctaa ttttgagata actagacaag atgttatttc cataaagcat
     4021 gagttgtatg atattgagta tctacttagc gctcataatt caagctgtaa agtacttgag
     4081 tattttatca ataagggatt ggttgatgta aacacaaagt tcaaaaaaac taatagtggg
     4141 gattgtatgt tggataacgc aataaaatat gagaatgcag aaatgataaa actattattg
     4201 aaatatggtg caacatctga caataaatat atttaatcaa aattgaatat cgtttagcaa
     4261 aaggcagtat ttgcacaaaa tatcgatata ttgtttattg tcagtatggc tcaatgtgat
     4321 aatagctgga aagtttgatg ggtttcgccc cgttgtagcg gtagtcgaac tggaaggtct
     4381 tcaggcactg cttacagcga tagagcagcc cccctgaact ggaatggccg ttccggatac
     4441 ccccctgagt ttcagagtaa cggtgacaaa ccacatcaat ctttgccatc aatcatccaa
     4501 agggcaaaga gtacaacaac actaagtctg cgtcacaacc catcaatgaa aggaatatat
     4561 acatatgcca tcagtaaatt taatcccatc aaggaaaata tgtttgcaaa atatgataaa
     4621 taaagacaac gtctctgttg agacaatcca gtctctattg cactcaaaac aattgccata
     4681 tttttctgac aagcggagtt ttttattaaa tctaaattgc caagttaccg atcactctgg
     4741 aagacttatt gtctgtcgac atttagcttc ctactggata gcacagttta acaaaagtag
     4801 tggtcacgtg gattatcatc actttgcttt tccggatgaa attaaaaatt atgtttcagt
     4861 gagtgaagaa gaaaaggcta ttaatgtgcc tgctattatt tattttgttg aaaacggttc
     4921 atggggagat attatttttt atattttcaa tgaaatgatt tttcattccg aaaaaagcag
     4981 agcactagaa ataagtacat caaatcacaa tatggcatta ggcttgaaga ttaaagaaac
     5041 taaaaatggg ggggattttg tcattcagct ttatgatccc aaccatacag caactcattt
     5101 acgagcagag tttaacaaat ttaacttagc taaaataaaa aaactgactg tagataattt
     5161 tcttgatgaa aaacatcaga aatgttatgg tcttataccc gacggtatgt ctatatttgt
     5221 ggacagacat actccaacaa gcatgtcctc cataatcaga tggcctgata atttacttca
     5281 ccccaaagtt atttatcacg cgatgcgtat gggattgact gagctaatcc aaaaagtaac
     5341 aagagtcgta caactatctg acctttcaga caatacgtta gaattacttt tggcagccaa
     5401 aaatgacgat ggtttgtcag gattgctttt agctttacaa aatgggcatt cagatacaat
     5461 cttagcatac ggagaactct tggaaacttc tggacttaac cttgataaaa cggtagaact
     5521 actaactgcg gaaggaatgg gaggacgaat atcgggttta tcccaagcac ttcaaaatgg
     5581 gcatgcagaa actatcaaaa catacggaag gcttctcaag aagagagcaa taaatatcga
     5641 atacaataag ctgaaaaatt tgctgaccgc ttattattat gatgaagtac acagacagat
     5701 acccggacta atgtttgctc ttcaaaatgg acatgcagat gctatacgcg catacggtga
     5761 gctcattctt agcccccctc tcctcaactc agaggatatt gtaaatttgc tggcctcaag
     5821 gagatatgac aatgttcccg gacttctgtt agcattgaat aatggacagg ctgatgcaat
     5881 cttagcttat ggtgatatct tgaatgaggc aaaacttaac ttggataaaa aagcagagct
     5941 gttagaagcg aaagattcta atggtttatc tggattgttt gtagccttgc ataatggatg
     6001 tgtagaaaca attattgctt atgggaaaat acttcacact gcagacctta ctccacatca
     6061 ggcatcaaaa ttactggcag cagaaggccc aaatggggta tctggattaa ttatagcttt
     6121 tcaaaatagg aattttgagg caataaaaac ttatatggga ataataaaaa atgaaaatat
     6181 tacacctgaa gaaatagcag aacacttgga caaaaaaaat ggaagtgatt ttctagaaat
     6241 tatgaagaat ataaaaagct gaatattatc tcgtggtgat acactgaatt tgaggtaacc
     6301 ggctcattta aaccgtctgg tctgttacct ccggttccac aaaaataatg tccatcattt
     6361 tgaagtggac actatcgtat ggaat
//
LOCUS       DAJVPP010000165         6322 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVPP010000165 DAJVPP010000000
VERSION     DAJVPP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.165"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(296..1198)
                     /locus_tag="OQM34_003794"
     CDS             complement(296..1198)
                     /locus_tag="OQM34_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCS1454044.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OQM34_003795"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OQM34_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCS1454045.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OQM34_003796"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OQM34_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCS1454046.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OQM34_003797"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OQM34_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCS1454047.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OQM34_003798"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OQM34_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCS1454048.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKELLARRANNPLP"
     gene            6040..>6322
                     /locus_tag="OQM34_003799"
     CDS             6040..>6322
                     /locus_tag="OQM34_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1454049.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIRFCGF"
BASE COUNT         1420 a         1623 c         1647 g         1632 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacacgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgag catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagcctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgaaccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agttgctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cg
//
LOCUS       DAJVPP010000166         6203 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVPP010000166 DAJVPP010000000
VERSION     DAJVPP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.166"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..163
                     /locus_tag="OQM34_003800"
                     /pseudo
     CDS             1..163
                     /locus_tag="OQM34_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            689..1867
                     /gene="ltrA"
                     /locus_tag="OQM34_003801"
     CDS             689..1867
                     /gene="ltrA"
                     /locus_tag="OQM34_003801"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1454050.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1950..2129
                     /locus_tag="OQM34_003802"
                     /pseudo
     CDS             1950..2129
                     /locus_tag="OQM34_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2159..2641)
                     /locus_tag="OQM34_003803"
                     /pseudo
     CDS             complement(2159..2641)
                     /locus_tag="OQM34_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2897..3877)
                     /locus_tag="OQM34_003804"
     CDS             complement(2897..3877)
                     /locus_tag="OQM34_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCS1454051.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3877..5076)
                     /locus_tag="OQM34_003805"
     CDS             complement(3877..5076)
                     /locus_tag="OQM34_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1454052.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5398..5736)
                     /locus_tag="OQM34_003806"
                     /pseudo
     CDS             complement(5398..5736)
                     /locus_tag="OQM34_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5803..>6203)
                     /gene="istB"
                     /locus_tag="OQM34_003807"
     CDS             complement(5803..>6203)
                     /gene="istB"
                     /locus_tag="OQM34_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCS1454053.1"
                     /translation="RVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIID
                     EIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRIL
                     HHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1680 a         1427 c         1483 g         1613 t
ORIGIN      
        1 gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta aagtccatgc
       61 cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga ctgcccggct
      121 gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat aagttatcca
      181 tgctctgagg tgggtggagg tgggccctcc ggtcagggtt tacaggagca cagaccaaac
      241 gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa aaggcagaga
      301 tctactgtca cgtggggttc gggaagctga acgaaagtaa actgtccgat gaagcatcga
      361 aatcttagat gatgccaaaa tggggccgta cagccgtccc ctgataagcg cagaagacac
      421 ctgcttactg actgcgcggc atccggtgtg aaggcagcag gatcccgggc cagaccatct
      481 cagggaacgt gagaacctgt ttccggcacg tatcgggaaa caggggcgga cccattcgta
      541 gtagtgatga aagatctgta atgggtctgg agcgaaggga tggggatcac attcattgca
      601 gaagacacaa cccaaaaggg gatgacgtaa ctgatgcaac gaaaatcatt tgaaatacca
      661 aaagcactgg tctgggcatc atatctggat gtgcgtcgca acaagggagc cccccggatg
      721 cgacgggcag actttgaaaa tgttcgacca gcaacgtgat ggcaatctat acaagatctg
      781 gaaccggcta tgttcgggaa catggtttcc gccaccagtg ctggaaaaac ggatcccgaa
      841 gtcgaatggc aaggaacgca ttctggggat cccaacagta tccgatcgaa ttgctcaggg
      901 agcgataaaa cttttcatgg aagaaaagct tgacccgatt ttccatgctg attcatatgg
      961 ttaccgacca ggcaaatcag ctcatgacgc tctgaaacaa tgtgccatcc ggtgctggcg
     1021 ttatagctgg atactggaag tggacatcag tgcgtttttc gaccatgtga gacatgatct
     1081 ggtgctcaaa gctctggaac accacgggat gcctaaatgg gtcatcctgt actgtcgacg
     1141 ctggatggaa gcgccaatgc agagttgtga aaatggagaa ttaataaccc gaacacgagg
     1201 cactccgcag ggcggggtca ttagtccgct acttgctaac ttgttccttc actatgcatt
     1261 tgatttgtgg atggaaagag aatatcgggg ggtaccgttt gagaggtacg ctgacgatat
     1321 tgtagtacat tgttcacgaa tgagtgatgc aacaaggcta aagaacaggt tatcggagcg
     1381 cttctcggaa gtcgggctgg tcctgaatgc agggaaaacg aatatcgcct acattgacac
     1441 gtttaaaagg agaaacgtcg caacgagttt cacctttctc ggatatgact tcaaagtgcg
     1501 tacgctgaag aatttcaaag gcgaacggta ccgaaaatgc atgccgggtg cgtcaaatgc
     1561 agcaatgcgc aaaataacag aaacaatcaa gaagtggcgt atacatcgct caacagctga
     1621 gagtttgctg gattttgcga ggcgctacaa tgcgatagtg agaggctgga tcgagtacta
     1681 cggaaagttc tggtccagaa atttcaacta tcgactgtgg agtgcaatgc agtcacgtct
     1741 gctcaagtgg atgcagtcta aatacagact ttcgaaccgg aaggctcagc ggaagctgac
     1801 gctggtaagg aaggagtatc cgaagctatt tgttcactgg tatttactac gtgcatcaaa
     1861 tgagtgatca agagccgtat gacgcgagag tgtcacgtac ggttccgtga gcagccgggg
     1921 agtgaaattc tctccggctg actcgactcg cgggttccga cagcgatgac gaacatgcgg
     1981 cggtgttgta ctcgctgatc ggcacatgcc gtctgaacaa tgtggaacca gaaaaatggc
     2041 tgcgttacgt cattgagcat atccaggact ggccggcaaa ccgggtacgt gatctgttgc
     2101 cctggaaagt tgatctgagc tctcagtaaa tatcaatacg gttctgacga gccgcttact
     2161 tttggcgtaa ttctgctgcg cctttactga acacaatgcc gtattcagca agaagtccac
     2221 gaatgtgatt tatcagttgt gtcctttcca ttgaaaccaa ccgacgcata ttcaaaaaag
     2281 tctggacgtc ctgttgctcc agagttttta ttggtacggg tcgtatagtt ccatgttggc
     2341 aggcctcagc tattgcctgt gcgtcgttat aatcattttt ctggcaccgc tgataagctt
     2401 taacatgttg tgccggaagc aatattacat catggtcgag tttcctgatt tcccgggccc
     2461 agtaatgaga tgccccgcca gcctccatcg caacagtcat tttaggctgt tgcaccagcc
     2521 agttaagcag ttgattgcga gaaagtttgg tctttcctgc gggtttgcca tgagcattga
     2581 tactgaaaag ataaaagttc gttttagcca gatcaatgcc ggtgattttc tgttcagaca
     2641 tggtatcccg cttacttttg ttgttaaagt ttatccatca tggcgcaatt gaaggctgcc
     2701 gtgaaagagg gggaagacta tctcatcggt tactgttaaa cggctaagag aaactatctt
     2761 tttcaagcaa agaagatacg gtgaaacagg cgatttcacc gtgaaatact gttcaaaaaa
     2821 aacgctaata aaagcaaaat ttcttttaaa acagccaatt aaaaactggt gagttgctgg
     2881 cgaaaaactt gaattttcac agcgaattca gattatcttt tagtacaaga gcaatcatac
     2941 gatctaactt atcctgaact tccaacggta atctgccgaa ttcatatgag aacgtacgtc
     3001 cctttactcg ttttcgagca aatttatctt tactatcaaa tttccataac aaagttacaa
     3061 ccgctttatc ctttactcct gaatcaagca atgaagacat ttccttggtt ataagccgca
     3121 taatactgtt tttaacctcg tcttcagaaa gattgtcatc agcagtaata agctcaatac
     3181 tcggcgagat attcttcaca acttcatcaa tagatatatc acccagcctc agtcgttcac
     3241 ttaattcagc cagttgcctg taatcggcgt atgtcagctc agactgaaca ggaaaaagag
     3301 acacaaaatc tttaggtacg gacgctgctt ggagggcacg agtcacctta gctgccgaca
     3361 atccctctct ttccgcaatt tgcttctggt tcataccggc ctctttgaga cgtactaaac
     3421 gcaacccaat ttcacgaata ttatgttcaa gtgacgtctg caaatctttc gccagatgct
     3481 gagcttcaga aacagtaagc tcatcatccg ttacaagtac acgtaatcca accttgcata
     3541 gcaaagctgc tgcccgacga cgggaaccat ccaaaatctc aataagatcg ccagtccgaa
     3601 tcccaataca tggatagaat tgttgtaaac gtatagttct ggtgatgtct tttagtgagt
     3661 ccggcgtcag ggctgtctgt tcgcggccat tcacctcttc aaccacaaaa gtttgagtat
     3721 ccactttgtc atgatcaacc ttgatctcag taaattttgc ctttcggcca gtattgaggg
     3781 taaatacatg aactggagcc gatatttctt cagtattatt caggatattt gtattcagcg
     3841 tacggccaat agtcggtcgg tgctttctgt tttccatcag tagttggccc ttataaattc
     3901 aatacgatca aaaactgctt tagcgaaatc ttctgctgcc atacgtgcat ttttcaaagc
     3961 ttcaccactg ccaacataag taacaggatt cgcagatatc acggtatcaa acgactcgcc
     4021 gcttctctca aaaccatcaa gacgtggcat tgaaacatca agcatgtcac caccgaaaat
     4081 ctcttttgtc aggctatggc agtatttatg atctgactta ttcgccagtt tcgacatgaa
     4141 gccaatattt gcctgcaaac ggcatgaaca tccatcctgt tcaataatct gtacaagttc
     4201 aggtaaacga gccaaatatt taagcgtgga atggaaatca acttgtgctg gcggcaccgg
     4261 tgtaaacatg atatctgcgg cagcaatggc atttttcaaa aatgcgtcca gatgcgggcc
     4321 tgtatcaata aggatgaagt cgaaatcatg cttcagctta tcaataatat tctcacgcaa
     4381 tatcgcgtgt ttgttttgtc ctaatagatg ctcctcgcac agagtatccc agtttgatgc
     4441 aataaaggcg tcgtcaatag aagccggcat aacataaacg ccagggataa ctgacggaac
     4501 gataaaatct tccagtaatt cctcacgact cacgttctgc aacatagcct gcggtgcggt
     4561 tgtatcaacc agccccaccg catgcaagta gttcagaaac atagtggctg atgactgggg
     4621 atctaaatca aggagcagaa tacgtaaatc ttctgacagc aggtgaggat gagcacgcag
     4681 ggcgtgagct acggaaacac tactcacggt tttagacaca ccgcctttta gactaccgat
     4741 gaatatggaa tatgcctcac tataacgatc gcgatattta ggaatgccac gatgtgcata
     4801 gatatcgata atgttctgga tggttaatgc atattttgtc gcggttcctg ctgggcgttt
     4861 ttcaaactga tagccctgag cctccatctc accgactgca ccatccacgc ttgcgcgcgt
     4921 taattttgga agcttagcta cagcagactt tgaatagact tgataaaaaa catcagcctg
     4981 aaactcctgc ttttgctcct gaatctgttt tgttagggcg agcaacattt tatctgcacg
     5041 ctgcgcaacc ttacttaact gctcaaaact agtcattccc cctccgtgct atgtaggatt
     5101 ttaacttatt acaaaaaaaa ctgcattagc agggtaatgc atgcaagtac atattgtaca
     5161 tgcttatatt aaaaatgcgc gcattgctga tttattaatc atcggaatga atattactgt
     5221 aagatagtat gcctttccgg gagaaagacg ctgatttccc ccgaataatc cagttcacca
     5281 ccacactccg gacaacagct ttcttcaggc tccagatggt gtatttcgcg ggaaacatgt
     5341 tccggtaacg gacggcgatg acgtgactgt cgcaacaggc gggggtagtg cttccaatca
     5401 gtagaacatg tgcttttcaa tgaaggagcc gatgactttt tcgtggattt ccacggaacg
     5461 cgagaagcag attgttttac gtgccagccg cttgatgcgg gtgcggaggg tccggttgtt
     5521 acgtgcaata cgttgcgtga agattttgcc ggtcaggtgt ttctccttcg gcacttctct
     5581 ggcgtagctg ccccagtcat cgctggtcac catgccgata cagaacggag tcaacagggc
     5641 taacagttcc cgacaggtct catcattccg tggcccgaaa gtgtaagcca acacaccgcc
     5701 agttttagtg ttgtacgcat accagagcca gtgttgtgtc aacgacggat gaaaagtgat
     5761 ccacttatat ctccaccaac ggcccaatat tgatccaccg ttttactcag gattagcttc
     5821 tgctataacc ccggcctttc gtttctgtct gagtcgatag ctttctcctt tgatttgaac
     5881 gacatgtgag tggtgtaaga tacggcccag catcgctgag gtcagtgctg catcaccggc
     5941 gaacgtttga tcccactgcc cgaacggtag attggatgtc aggatcattg cgctcttttc
     6001 gtaacgttta gcgatgacct ggaagaacag ttttgcttct tcctgactga acggcagata
     6061 gcctatttca tcaatgatga gcaggcgggg ggccattact ccacgctgaa gcgtcgtttt
     6121 ataacggccc tgacgttgtg ccgtagataa ctgaagtaac agatctgctg ctgttgtgaa
     6181 gcgaactttg atacctacac gga
//
LOCUS       DAJVPP010000167         6200 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVPP010000167 DAJVPP010000000
VERSION     DAJVPP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.167"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OQM34_003808"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OQM34_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1454054.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OQM34_003809"
     CDS             2089..2238
                     /locus_tag="OQM34_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454055.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OQM34_003810"
     CDS             complement(2983..3423)
                     /locus_tag="OQM34_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1454056.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OQM34_003811"
     CDS             3641..4030
                     /locus_tag="OQM34_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454057.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OQM34_003812"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OQM34_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OQM34_003813"
     CDS             complement(4585..5421)
                     /locus_tag="OQM34_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1454058.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="OQM34_003814"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OQM34_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1454059.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1432 a         1617 c         1642 g         1509 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJVPP010000168         6189 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVPP010000168 DAJVPP010000000
VERSION     DAJVPP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.168"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(104..1813)
                     /locus_tag="OQM34_003815"
     CDS             complement(104..1813)
                     /locus_tag="OQM34_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCS1454060.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="OQM34_003816"
     CDS             complement(2245..2964)
                     /locus_tag="OQM34_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCS1454061.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3089..3645)
                     /locus_tag="OQM34_003817"
                     /pseudo
     CDS             complement(3089..3645)
                     /locus_tag="OQM34_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3629..3887
                     /locus_tag="OQM34_003818"
                     /pseudo
     CDS             3629..3887
                     /locus_tag="OQM34_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3996..4574)
                     /locus_tag="OQM34_003819"
     CDS             complement(3996..4574)
                     /locus_tag="OQM34_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454062.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(4995..5232)
                     /locus_tag="OQM34_003820"
                     /pseudo
     CDS             complement(4995..5232)
                     /locus_tag="OQM34_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5419..5848)
                     /locus_tag="OQM34_003821"
                     /pseudo
     CDS             complement(5419..5848)
                     /locus_tag="OQM34_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5896..6081)
                     /locus_tag="OQM34_003822"
     CDS             complement(5896..6081)
                     /locus_tag="OQM34_003822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454063.1"
                     /translation="MDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
BASE COUNT         1717 a         1412 c         1096 g         1964 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgatcatggt gggaaggctt agaacgccat attcagtttc ccatagtgga
     3121 acatcactcc cagcttaaag cgctcccggt tgcggtagcc cttggctttt attctcagca
     3181 gcctgatctt gctgttcagt gcttccgcat ttccgtttga tacgccatgg cgcatggcgt
     3241 tcaggatacc gtacaacctt ttccctatcg ttttagccac attcttcatc atggggacgt
     3301 cactgttggc cgcagccatc tctgccagtc gttgcgtctt tcctcgctcc atggtctgct
     3361 ccagatatcc tttgccagtt ctttcagcgc ccagcacagg ctcgtcagct tcatctgcgc
     3421 acgcagccat atcagctttt cctgcctgga ttcggtcatc catttatcgc tgtgctgcca
     3481 caggaagcga gttccctttg cctgacgacg actttcaaca ggaagatgcg gatgttcatt
     3541 ctggcgggtt ttatccacca cctcacccgg ttgtttcgcc acatgaaagc gatcgaaggc
     3601 tattttctca accgcattgg gtaagtggat gcgcgctgct cttagtttgc tgagccagtt
     3661 tagcattgcc ttctacatga ccgatgcctg agccggtata ccgaacttta ttgtcctcaa
     3721 ccagtcatgt tataagcaag aaatgcactc agcggataga gcgacataat ctgaacctgc
     3781 ggacacatct taagcggctt acccgcaaaa ccatatgctt ctcaaagtct gatgatatgc
     3841 actacaagat catcggctgg tatctgacaa tcaaccacca ccactaaatc tgcgtcacga
     3901 cctgcaactg agtctggttg aacaggctga ggccctgctg aagcaacgcg aacctctcga
     3961 ctcttgttgg attttaccaa atagtttttc taaaattaaa cacatttata taagttcttt
     4021 gctgaacaaa acgcagcccg ccatcctttt tcattcataa attcagcaat ggcagataaa
     4081 actttttcat caagcatata aaaaataatg ccagtagact caatatgcct catacaaata
     4141 aggccgcaca tatccgtaat atatttgtca atgctgctac catttgcatg gtaatatgtc
     4201 atgctattat ctgatgattt tacatatgca gtatgacata tttttccttc acgattaaca
     4261 aaactgacaa aaccactttc accaggagta atatataaag atgaatttaa cgtgcgctca
     4321 ctaatattca ttaattgttg aaattcaatg gaatagtttt cagttaatgt atctattatc
     4381 ttggctttgt ctttctttgt tatctcattg tctttttcgg caagaaaaat tgccacttta
     4441 tgacatgcat tcagctcgtt aattttacct gataaaactg cttttcttac cacatcgttg
     4501 aactcatcga tagtgtgttt tttattatat atatcatgaa aaatatagcc taaagactta
     4561 aacgagactt tcattttata ttctccttaa atattaacag gatatatata tcacattttc
     4621 ctctatattt tcagaaacca gctatcattt tccccattac tggtacgtcg cttcagatat
     4681 ttccgcagca agcgataacc ctgcgcacta tgaggatgcc attcattata atgtttgaac
     4741 accttcgcga gactacttta ctgctgttaa cacgtcaggt tcgaacataa cactgattta
     4801 atcatatttc atcgttttga cgaagttttc tgccattcca ttttgcaccg ccgtgtgttt
     4861 gggggcaagg ccgaccatct gtgtttcatt cgccagatat aagtgatgag atggcattct
     4921 ccctccttca cggcagaagc attttgaccg tttaggggat gcggacgatt ttcatgcctg
     4981 ctaagatact ttcgttactg atgctgtccg ttttccacca tttcatcatc actgattggc
     5041 gttgtgtttt tggtcagcac tccagcttta cgtttctctt ttaaccggta actctctcct
     5101 ttgatattca gcgtgtagca gccgatctag gatcgctgtt gccagcacgt gatctccgaa
     5161 catttctccc cagtctgcga cccctttgtt tgacgtcagt atgatgctcg ctttttcata
     5221 tcgacagttc atcgatagga atttaaatcc ccaaaagatt aaaaaagcac cgaagaatgc
     5281 gcgtttattc aacacaacct gatcatcaag taatcggtat agacacttaa tattgcttac
     5341 attgattgat tcaatgtatc tctgatggtt ttttgctccg ttcccctcaa catggacgca
     5401 acttcccctc tgcgactctc agccgcacca ccgtattcga gccagcggct catgcatcat
     5461 aacctgctct gccagacggt aacccctctt cagccccgta aaccgcatct gacctccgcg
     5521 cagctcgcac ttcagcgggt caaccttcag tcactgctga tacatccctc tccttgtgat
     5581 ttgcctcgcc gtttttctca ctgtctccgt tatggcgtac accacatctt ccagtaatcg
     5641 ccgcttcacc ggactcagga ccccgtatca ccatccggaa ccccttatcc gccacatgcc
     5701 aggagaacct ttccataaac tcgtcgccac tcatcacaag gttttcttct cgttttgtcc
     5761 ggtggctgtt gtaacgcagg ccaatttcat cctgaccggc ataatgttcc aggcggctca
     5821 tctgcaccgg ctgcttctcc agccagttaa gccatcggtt tccctgctga aaaatatcgg
     5881 caaaacgggg aagcatcaga aaggcggaat aacgccatcc ggccagtgaa ccgtaccgca
     5941 ttcagggcaa tacatgccgt cggcgttgat accggcagga attgtcgcaa attcccgctc
     6001 catgcagcgg gcaatttccg gatacccttc gtcatcaaca cgtacaaacc agaagaccag
     6061 ttttttgttt ccggcatcca caaagaacgg aatattcagg tctgcgcaac actcaacggc
     6121 atcgtcaaaa ttatcaaagc gcagaacctc agcttcttcg taaccggctc atttaaaccg
     6181 tctggtctg
//
LOCUS       DAJVPP010000169         6108 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVPP010000169 DAJVPP010000000
VERSION     DAJVPP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.169"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            230..1054
                     /gene="gadX"
                     /locus_tag="OQM34_003823"
     CDS             230..1054
                     /gene="gadX"
                     /locus_tag="OQM34_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1454064.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1422..2150
                     /gene="gadW"
                     /locus_tag="OQM34_003824"
     CDS             1422..2150
                     /gene="gadW"
                     /locus_tag="OQM34_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1454065.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2513..5626)
                     /gene="mdtF"
                     /locus_tag="OQM34_003825"
                     /pseudo
     CDS             complement(2513..5626)
                     /gene="mdtF"
                     /locus_tag="OQM34_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5651..5908)
                     /locus_tag="OQM34_003826"
                     /pseudo
     CDS             complement(5651..5908)
                     /locus_tag="OQM34_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1609 a         1434 c         1452 g         1613 t
ORIGIN      
        1 agcgattacc tttaatgtca cgaaaaggga ttatttgctt gctattaatt tcactgcgtt
       61 cttaaacacg tttttgtatt ataaatccag tcatcctgcc cgtagaacat cacacattat
      121 catcctgttc tcccgcttcg tttaaattta tttatcaatc aatttgactt aagagggcgg
      181 cgtgctacat taattaacag taatatgttt atgtaatatt aagtcaacta tgcaatcatt
      241 acatgggaat tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg
      301 tgaatatcgc tattttaatg gcggtgacct ggtttttgcg gatgcaagcc aaattcgagt
      361 agataagtgt gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctgcc
      421 gatgctcaag gaggaggcat taaatcttca tgcacataaa aaaatttctt cattactcgt
      481 tcatcactgt agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa
      541 gaatcttcgc tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt
      601 ttttcttgag gatgagcact ttataccgct gcttttgaac gttttgcaac cgaacatgcg
      661 aacacgagtt tgtacggtta tcaataataa tatcgcccat gagtggacac tagcccgaat
      721 cgccagcgag ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaagagac
      781 atcatattca cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat
      841 acatggcttt tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt
      901 catttacgtc tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc
      961 gcagggattg ccgaaccgtg actcggcggc aagtattgtt gcgcaaggga atttttacgg
     1021 cactaaccgt tctgcggaag gaataagatt atagagtttt actcagacat aaaaaaaacc
     1081 cggcataggg gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac
     1141 actccctgtt ggcacgggaa actttgtgct ctcagtaagt taaatataac ttttactgga
     1201 aataagatca gccatttttt tataaacata agctatacgc tgtgcgaaaa tatattcgtg
     1261 ctgcatttac ttattatcaa ttaactgtta tgcaaaacta ctttgtggat aaattttggt
     1321 cctaccaaat ctggcagttt ttgcgctaag aaacagtctg tcatcatttc attagtatac
     1381 tgaaattgaa ataatcgcag tatgaaatat aagggataat catgactcat gtctgctcgg
     1441 tgatcctcat tcgtcgttca ttcgatattt atcatgaaca gcaaaaaata tcgctgcata
     1501 acgagagtat cctgctgctg gagaaaaatt tggcagacga ttttgcgttt tgttcaccgg
     1561 atacgcgacg actggatatc gatgagctga cagtttgcca ttacttacaa aatattcgtc
     1621 agctaccacg caatttaggg ttacatagca aagaccgtct gttaattaac cagtcacccc
     1681 ccatgccgct ggtgacggcg atttttgata gcttcaatga atccggggta aattcaccga
     1741 tactgagcaa tatgctctac ctttcctgtt tatcgatgtt ttctcataag aaagaactga
     1801 tccccttact tttcaatagc atcagcactg tttcaggaaa agttgaacgc cttattagct
     1861 ttgatatcgc caaacgttgg tatctgcgcg atatcgcgga aagaatgtat accagcgaga
     1921 gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata ttactcgctt
     1981 ccaggatgtc gatggccaga cgattactcg agttacgtca aattcctctg catactattg
     2041 cggaaaaatg tggctatagc agtacgtcgt actttataaa cacatttcga caatattatg
     2101 gtgtaacgcc acatcagttt gcgcaacatt cgccaggtac cttttcctga catattttgc
     2161 atttgaatat tggtcaggat ctcacacctg cttcatgtga aactcttccc tgatgatttc
     2221 tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt atttttagga
     2281 ggaaaagact cggcatgttt gggattatta agctgacaat tcatacgatt acgggaatgt
     2341 gggtcagtat tgtgttgttc aaattgatga cgaatggctg gagtggtttc tactttcagt
     2401 gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt gaatggttag
     2461 caggaaagag taaggctgaa ccttcacgtt caaccttact ctcatttaca cgttacgctt
     2521 ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaacg aagtaaattg
     2581 ccagcactgt tgcggcaaac atcccgccca tcacgccggt acctaccgcg ttttgcgcgc
     2641 cagaaccggc accatgactg ataaccagcg gcagcacgcc gagaataaag gccagagagg
     2701 tcatcaggat tgggcgtaaa cgcatccgcg ccgcttcgat gattgcctct atcggcgttt
     2761 tcccttcttt ctgcatcatc tcaacggcaa attcgacaat caggatggcg ttcttggcgg
     2821 aaagcccgat ggtggtcagc aaaccaacct ggaagtagac gtcattactt aagccgcgca
     2881 gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc accgagaacg
     2941 gaattgacca gctctcatag agtgcggcga gggcgaggaa caccacgacc agtgaaatgg
     3001 catacagtgc aggagcctgg tttgaggata acgcttcctg atacgacagt ccggtccatg
     3061 agtagccgac gcctgccgga agtttagcga ccaggtctgc cataaatttc atagcgtcac
     3121 cggtactttt cccggccgcc gcttcaccta aaatctccat tgacgggatg ccgttgtagc
     3181 gttccaatcg cggtgaacca taggtccatt cggtagacga gtaggcagaa agcggtgcca
     3241 tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac atacggaacg
     3301 gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag tcgttcacgt
     3361 agctgctgcc gaaggaggtg gaaattgtct ggttgatatc agacagcgcc acacccattg
     3421 cctcagcttt cgcggcgttg acgttcactt tgaacatcgg cgtatcttcc aggccgttcg
     3481 ggcgtacccc ggtgacctgg tcaggcgatt gcgctgccag tgataacagc tcgtttcgtg
     3541 cctaggttag tttttcgtgc cccaggttac cgttgtccag cagttccata tcaaaacctg
     3601 acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg gctttattga
     3661 tactgcttaa cgcaatcatt gcccgctgaa tgatcgcggt aaccgagttt tcctcaccga
     3721 cacgttcaga ccacggcttg agactgataa acgccaggcc gttgttttgc ccctggccgc
     3781 tgaagccaaa gccgccaacg gtaaacaccg actgaacatt atctttctct tttgtaagat
     3841 aataatccgt cacctgttgc agtactttcg tggtgttaac catggtggca ccggaaggta
     3901 actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag gtcggtgtac
     3961 gcaggaacag caccgccatc ccggcgcaaa tcagcaggta gaccaccatg tagcgaccgg
     4021 tacaacgcaa cagcgagcgg gtgctatcgg tatagtgctg agttgatttt tcaaacagcg
     4081 tgttgaagcg tgcgaacagg gcgttaggtt tgtgaccgcc ttccggcgtg gctttcagaa
     4141 tggtggcgca cagggcaggg gtcaggctca ttgccacaaa tactgaaagc cgcatggagg
     4201 agatcagcgt gatggagaac tggcggtaga tctccccggt tgcaccgctc ataaaggcca
     4261 tcggcataaa cactgcagaa agaacaacgg caatcccgac cagcgcacgt tggatctgcc
     4321 ccatcgattt atgcgtcgct tccttcggcg gtagcttatc ttccgcaatg acacgctcga
     4381 cgttctccac caccacgatg gcgtcatcca ccagtaaccc tatcgccagc accatcccga
     4441 acatagtcaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa
     4501 taaccaccgg tacggcaatt gtcgggatga tggtggcacg gaaattctgc aaaaacagat
     4561 aaatgaccag gaagacgagg atgatagcct caaccagtgt tttgaaaact tcctgaatag
     4621 aaatttcgat aaacggcgtg gtgtcgtaag gataaaccgt cttcagactt gcggggaaat
     4681 aggctgataa gcggttcagc tcctctttga ctgcccgcga ggtatccagg gcgtttgctc
     4741 cggcagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc gccacggtgg
     4801 aataatcttc cgccccaagt tcgacgcggg cgacatcacg cagcagcact tgcgaaccat
     4861 cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga cgcgtctgca
     4921 caatgatcga ggcgtttagt tgctggtctg ccgcctgtgg catgccaccc agttgaccac
     4981 cagaaatctg gttgttttgc accttaatct gggaaataac atcggaaggt accaggttat
     5041 atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa ccaaagagct
     5101 gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac gctacatagt
     5161 ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaacgccgct accatcagga
     5221 tattactgct cgacttatcg acgctaatcc cctgttgctg caccgcttca ggtaatgaag
     5281 gcatagcgag ttgcagttta ttttgcacct gaacctgtgc gatatcagga gatgtcccag
     5341 tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa gacatgtaca
     5401 tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag tcttctaccg
     5461 tttgcgcatc ggcacccgga taggtagcgc tgacggtaat ggtcggtggc gcaatctgcg
     5521 gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc ataataatgg
     5581 caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa gtccccttaa
     5641 gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag gaaattgctc
     5701 gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga tcgccagcct
     5761 gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca atttcgcgta
     5821 gctgcacgac atcgtcttta tccagaatga gcgccgttgc tttaccctgg gcgttgtggg
     5881 tgacgccttc ctgcggcacc agtaatataa agcgcgcaaa attattcaag atatttttga
     5941 aaatcattca agataggcta tcgcgtgtga tagcaaaaac attcaagata aaaatgcttg
     6001 attattaagc aatcaggaat gggagtatta agtttcgtgt ttccagtaaa accagagagt
     6061 attgaaatag ctatcaccta actttatcag gcgatagctg gtagatga
//
LOCUS       DAJVPP010000170         5864 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVPP010000170 DAJVPP010000000
VERSION     DAJVPP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.170"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            142..1566
                     /gene="sbcB"
                     /locus_tag="OQM34_003827"
     CDS             142..1566
                     /gene="sbcB"
                     /locus_tag="OQM34_003827"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1454066.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1609..1836)
                     /locus_tag="OQM34_003828"
     CDS             complement(1609..1836)
                     /locus_tag="OQM34_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1454067.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1850..2908)
                     /gene="yeeE"
                     /locus_tag="OQM34_003829"
     CDS             complement(1850..2908)
                     /gene="yeeE"
                     /locus_tag="OQM34_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS1454068.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3087..4445)
                     /gene="plaP"
                     /locus_tag="OQM34_003830"
     CDS             complement(3087..4445)
                     /gene="plaP"
                     /locus_tag="OQM34_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1454069.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4435..4497)
                     /gene="yoeI"
                     /locus_tag="OQM34_003831"
     CDS             complement(4435..4497)
                     /gene="yoeI"
                     /locus_tag="OQM34_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1454070.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4658..5641)
                     /locus_tag="OQM34_003832"
     CDS             complement(4658..5641)
                     /locus_tag="OQM34_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1454071.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5687..>5864)
                     /locus_tag="OQM34_003833"
     CDS             complement(5687..>5864)
                     /locus_tag="OQM34_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1454072.1"
                     /translation="HPARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELG
                     FEYQYPDPLVMPLE"
BASE COUNT         1605 a         1536 c         1386 g         1337 t
ORIGIN      
        1 catctggtac acaatatcac atggacaaca gcgcacagta aacaactata tttgacgttc
       61 gtagacatac tcaggtgaaa aaagggttgc ttttcgagtt ataacagaca gttaattaac
      121 tacagtggac aatgctagac aatgacagac acagataaac aacctacctt cctctttcac
      181 gattacgaaa cctttggcac gcaccccgcg ttagatcgcc ctgcacagtt cgcagccatt
      241 cgcaccgatg acgaattcaa tgtcatcggc gaacccgaag tcttttactg caagcccgcg
      301 gatgactatt taccccggcc tggagcagta ttaattaccg gtattacccc gcaggaggca
      361 cgggcgaaag gagaaaacga agccgcgttt gccgcccgta ttcactcgct ttttaccgta
      421 ccgaagacct gtattctggg ctacaacaat gtgcgtttcg acgacgaagt cacacgcaac
      481 gttttttatc gtaatttcta cgatccttac gcctggagct ggcagcatga taactcgcgc
      541 tgggatttac tggatgttat gcgtgcctgt tatgccctgc gcccggaagg aataaactgg
      601 cctgaaaatg atgacggtct accgagcttt cgccttgagc atttaaccaa agcgaatggt
      661 attgaacata gcaacgccca cgatgcgatg gctgatgtgt acgccactat tgcgatggcg
      721 aaactggtaa aaacgcgtca gccacgcctg tttgattatc tctttaccca tcgtaataaa
      781 cacaaactga tggcgttgat tgatgttccg cagatgaaac ccctggctca cgtttccgga
      841 atgtttgggg catggcgtgg caataccagc tgggtggcac cgctggcgtg gcatcctgaa
      901 aatcgcaatg ccgtaattat ggcggatttg gcaggagaca tttcgccatt actggagctg
      961 gatatcgaca cattgcgcga gcgtttatat accgcaaaag ccgatcttgg cgataacgcc
     1021 gccgttccgg ttaagctggt gcatatcaat aaatgtccgg tactggccca ggcgaatacg
     1081 ctacgcccgg aagatgccga ccgactggga attaatcgtc agcattgcct cgataaccta
     1141 aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg tagcgatatt cgcggaagcc
     1201 gaaccgttta caccttcaga taatgtggat gcacagcttt ataatggctt tttcagtgac
     1261 gccgatcgtg cagcaatgaa aattgtgctg gaaaccgagc cgcgtaattt accggcactg
     1321 gatatcactt ttgttgataa acgaattgaa aagttgttgt tcaattatcg ggcacgcaac
     1381 ttcccgggga cgctggatta tgccgagcag caacgctggc tggagcaccg ccaccaggtc
     1441 ttcacgccag agtttttgca gggttatgct gatgaattgc agatgctggt acaacaatat
     1501 gccgatgaca aagagaaagt ggcgctgtta aaagcacttt ggcagtacgc ggaagagatc
     1561 gtctaatttg aagccgccgc cgctggtacg gcggaggaca atgttgagtc aggctttttg
     1621 aacggtgatg ctccacgctg catcgccaat ctgctgataa tcggtgatgg catgtccttc
     1681 ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg caatcaaact caattaccaa
     1741 ctcatcgccg ctggccattt ctgccagtgc tgcttttgct tctatgagtg gaaacggaca
     1801 gacctgcgtc accacatcca gctttttaat cgccatcttt acaccttact taatttgccg
     1861 cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata cacaagccag gacgcagtcc
     1921 agactccgag aatcataaat acaaggccaa tccagccctg ccaggtcatc attgcagtca
     1981 taaccagtcc attaccgata gagcaaccac ctgcaatact ggcaccgaag cccatcagta
     2041 caccacctaa cccgctacgt aatgttgttt gtgcatcagc tgcgcgaacg cggaactcac
     2101 ggctcgcttt ggctgcaata aatgcctcca cgaagatccc taacaccagg aaaacgcccc
     2161 agttaatgta tttcacgtca cccgcgacca gaaattgaag aatattggcc gttggggaag
     2221 tgactcctaa cccaaacatg cgtccggttg cttcactcag gggccaggct aaaagcgcaa
     2281 tcaaaccgat gagtacagca gtgacaaagg gatgccagcg tttttcaaac agaatatgag
     2341 cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag ttttggcttc ttcaactctt
     2401 tcatcaccac ccagagcgtt atcaccagca aaacggtaac caacggccac acagacaaat
     2461 taaaagtctc agcaatagag ttatgttcag tagtgtagtg ctgcaaggtt tgatttaaac
     2521 cactggcgtg tggagaacgc atcaccgcac tcatcaccat ataagtgaaa agcgcgatcc
     2581 aactgccgat caatccctca cccgcgcgat accaggtccc ggtggcacat ccacccgcca
     2641 gaacaattcc cagcccgaaa agatacccac ctataacagt accaagccac gggaacgccc
     2701 cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa aacccccacg ctttgtgccg
     2761 aaattgcaat cagcaaggcg taaaacatgc gattattttt cgcgatatac atatcgcgaa
     2821 aaccaccagt caggcaaaaa cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa
     2881 ttagcccgct taatatcatt gaaaacataa attaattaac cagatgaatg ttaatgggga
     2941 aatttttcat gactggtgga atgcagacca ataaccaaat tctctaaatt agaacaaatg
     3001 gttattaatg aggatttcga taaaaaagaa taaaaaaacc ggagccaaat gttcattcga
     3061 ctccggttta ttattagaaa agatgattat gctacgtctt cgtactgcgg aaccggatta
     3121 cggaagctct tggtgacgca agccaggtaa atcagaccga tagcagccca gatcagaccc
     3181 agaaccattg agctttcttc caggttaacc cacagcgcac caacggtcag cgcaccacac
     3241 atcggcagga acagatactg gaagtgatct ttcagcgtct tgttacgctt ctcacggatc
     3301 cagaactgcg agatgaccga caggttaaca aaggtgaacg ccaccagcgc accaaagtta
     3361 atcagcgccg ttgccattac caggtcgaag ttgattgcca gcagggcaat cgcgccaacc
     3421 aggatgatgt tcatcgccgg agtacgccat ttcgggtgta cataaccgaa gaagcttttc
     3481 gggaacacgc cgtcacgacc cattacgtac atcagacgcg ctacgcctgc gtgcgccgcc
     3541 ataccggacg ccagtacggt aatggtggag aagatcagcg cgccaacctg gaacgcttta
     3601 cccgcaacgt acagcatgat ttcaggctgt gacgcatccg gatctttaaa gcgagagata
     3661 tccgggaagt acagctgcag gaagtaagtt gcaaagatga agatcatgcc gccaatcagc
     3721 gcggtcagga aaatcgcacg cgggatcacg cgctctgcat ctttggtttc ttccgacaga
     3781 ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc
     3841 gggataacat gcgcatcgcc agaccagaac ggacgcgtgc tcgccagcgt accagcgcct
     3901 tcaccttcaa atacgccata aacaaccatg cccagaatca ccgcgatcag cactacctgc
     3961 aacacgacga ttacagtgtt gacgttcgct acggatttca gactacgcag gttaaaggcg
     4021 gtcataaagg ccaccagcgc caccacaaac atccacgatg gaatggaagg caccagagct
     4081 tcaaaataga ttttcgccag cagaatgttg atcatcggcg cgaacagata gtcgagcaga
     4141 gaagaccaac ccaccataaa gccgacagtc gggctaatgg atttctgggc gtaagtgtat
     4201 gcagagccag cagaaggata gcggcgaacc agcttcccgt agctcagagc cgtaaacagg
     4261 atcgcaatca atgcgaacgc ataggctgtc ggcacatgac catccgtgag gcctgaaacg
     4321 ataccaaatg tatcaaacag cgtcatcggc tgcatatagg ctagacccat cattacaacc
     4381 ggaactaacg taagcgtttt acgcaattcc acgcgagagg tgtttggagt aacgttatgc
     4441 gacatggtta ttctccttta cggtgataac cgtcgcgtaa gcaaaaaatt gccccatttt
     4501 tttggattcc tcagcgacaa caactgtcga tttttagtaa atatctatcc ggtacgaagc
     4561 ccggcctctt ggtatgaatg attggtttga agcaaaaaat aaccgacgct aattaaacgt
     4621 cggtttttaa tcattttttg acagcggcgc attgtgccta aatggggggg aaatgacaag
     4681 agaatgagag gcttgtcata ataatttttc tttgaatggc tgattttccg tcatcagatt
     4741 ttgcataaac accggcgatc gcactatttg ctaaaatttc atctcgccac cagccgctgg
     4801 caagccctgc ggtctgttca ttccagccaa tccagacagg ttcaaagaga gtttgggcga
     4861 ccactttctt ctcgattaac gcgccactat cgagaaaacg ttgcgccaga taccgtggta
     4921 aatagccgca gccaaggccg ctaatttgca gctccagttt ggttttaaaa tcgaaaacgg
     4981 taatggcttc ttgttcgtcg agaagttgtg aggccgtaga agcgcctgcc tgcgcagtgt
     5041 cccccaccac aatcgcccga tatcgtttaa tgatacgacg gtttaacggt tcttcttcct
     5101 gggctaacgg atgatgaggc gcgaccgcaa agacttgttc aagatcgccc aaccgcgaga
     5161 aaccaaactc actggaagat ggtggctcat gcatcgcgcc aacgataata tccgctcgcc
     5221 cctgagtcag ggcatcccag gaaccaccga gtacgccatt gataaacttc aggcgcgtca
     5281 cgctatgatg ttgataaaaa gcttcaatga gaggcgcaag cagagaaaaa ggaaacgtgt
     5341 cgtccacgcc aatcaccagc tcgttttccc agccttcatg cagtttgatc gcctgctttt
     5401 ccagttctcg cacggtatgc agaacttccc gccctttctc taataacatt tttccggtgc
     5461 gggtgaattt agcgcggtgt ccgctacgat caagcaattg aatatttagg tcgctttcca
     5521 gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta gagtttggct gacgccgccg
     5581 caaaactgcc ctctttttct atggcatcga ggatcatcaa cacatccagc agtggtttca
     5641 tacacgcccc cttgcagtgc atgcggcgtc ctgctggcgt gatcgtttac tccaacggca
     5701 ttaccagcgg atcgggatac tggtattcaa atcccagttc attacaaatc cgactgccat
     5761 caataatctt acctttgccg ctgtccagac tatttctgaa ctgcggaggt tgcagcccca
     5821 gtaaacgggc catctgcgga tagaaaacat tacgcgcagg gtga
//
LOCUS       DAJVPP010000171         5856 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVPP010000171 DAJVPP010000000
VERSION     DAJVPP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.171"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            18..1406
                     /gene="narU"
                     /locus_tag="OQM34_003834"
                     /pseudo
     CDS             18..1406
                     /gene="narU"
                     /locus_tag="OQM34_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            1488..5228
                     /gene="narZ"
                     /locus_tag="OQM34_003835"
                     /pseudo
     CDS             1488..5228
                     /gene="narZ"
                     /locus_tag="OQM34_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5225..>5856
                     /locus_tag="OQM34_003836"
     CDS             5225..>5856
                     /locus_tag="OQM34_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1454073.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVL"
BASE COUNT         1377 a         1563 c         1560 g         1356 t
ORIGIN      
        1 tgcatatgaa gaacaatatg gcactgcaaa atgagaaaaa tagtcgttat cttttacgcg
       61 actggaaacc agaaaatccg gccttctggg aaaataaagg aaaacatatt gctcgaagaa
      121 acctctggat atcagtcagt tgtctacttc ttgccttctg tgtctggatg ctatttagcg
      181 cagttaccgt taatctcaat aaaatcggtt ttaattttac taccgatcaa ctctttttat
      241 taaccgcatt accctccgtt tctggcgcat tattgcgtgt tccctactcc tttatggtgc
      301 ctatattcgg tggacgccga tggccggttt ttagtactgc aatcctgatt attccttgcg
      361 tctggctcgg aattgccgtg caaaatccga atactccttt tgggatattt atcgttatcg
      421 ctttgctatg cggttttgca ggtgcaaact ttgcttcgag catgggcaat atcagtttct
      481 tctttccaaa agccaaacaa gggagcgctc ttgggattaa tggcggatta ggaaacttag
      541 gtgtaagtgt aatgcagctg gttgcaccgc tggtcatttt tgtacctgta tttgcctttc
      601 tcggcgtcaa tggcgtaccg caggccgacg gttcggtgat gtcgctggcg aatgccgcat
      661 agatttgggt accgctactg gcgattgcca cgatcgccgc ctggtcaggg atgaatgata
      721 tcgccagttc acgcgcctca attgccgacc agctccctgt cttacaacgc ctgcatctct
      781 ggctgctgag cctgctttac cttgccacct tcggttcgtt tatcggtttt tctgcgggtt
      841 ttgccatgct ggcaaaaacc cagttcccgg atgtgaatat tctgcgcctg gcgttctttg
      901 gcccatttat tggtgccatc gcgcggtcgg ttggtggtgc tatttccgat aaattcggcg
      961 gcgtgcgggt gacgttgatc aactttattt ttatggcgat tttcagtgcc ctgctgttcc
     1021 ttaccttacc gggcacaggc tccggtaatt tcatcgcctt ttacgccgta tttatggggc
     1081 tgtttctgac cgcgggtctg ggaagtggtt ctactttcca gatgatcgcc gtcatctttc
     1141 gccagataac catttatcgg gtgaagatga aaggcggtag tgatgagcaa gctcagagag
     1201 aagccgtcac cgaaacggcg gcaacgctgg gctttatctc agccattggc gcagtgggcg
     1261 gcttttttat tccgcaggca tttggcatgt cgctcaatat gaccggctct ccggtcggcg
     1321 cgatgaaagt gtttttaatc ttctacatcg tttgtgtgct gctgacctgg ctggtttatg
     1381 gtcggcggaa gttcagccaa aaataagaga tcgatgcctg atgtacaacc atgtcgcatc
     1441 cgacaaggca tcagatccca attcattcct ggagcaggag tcatgtcatg agtaaacttt
     1501 tggatcgctt tcgctacttc aaacaaaagg gcgaaacctt tgccgatggt cacggacagg
     1561 tgatgcatag caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa
     1621 tcgtgcgttc cacccacggc gttaactgta caggctcctg tagctggaaa atctacgtta
     1681 aaaatggtct ggtgacctgg gaaatccaac agaccgacta cccgcgcact cgccctgacc
     1741 tgcccaatca tgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctttaca
     1801 gcgctaaccg cctgaaatac ccgctcattc gtaaacgact gattgaactg tggcgcgaag
     1861 ccctcaaaca acacagcgat ccggtactgg cgtgggcatc gattatgaac gatccgcaaa
     1921 agtgcctgag ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggcagg
     1981 aactaaacca gctgattgcc gccgctaacg tctggaccat caaaacctac ggcccggatc
     2041 gcgttgccgg tttctcgccg atcccggcga tgtcgatggt ttcttacgcc gccggaacgc
     2101 gttatctgtc gctgcttggc ggcacctgtt aaagtttcta cgactggtat tgcgacctgc
     2161 cgcccgcctc gccgatgacc tggggcgagc aaaccgacgt accggaatct gccgactggt
     2221 ataactccag ctacatcatc gcttgggggt ctaacgtacc gcagacacgt acgccggacg
     2281 cccacttctt taccgaagta cgctacaaag gcactaaaac catcgccatt acccctgact
     2341 actctgaagt ggccaaattg tgcgaccagt ggctggcacc gaaacaaggc actgatagcg
     2401 ccctggcgat ggcaatgggg catgtgattt taaaagagtt tcatctcgat aatcccagcg
     2461 actactttat caactactgc cgccgctaca gcgacatgcc gatgctggta atgctggagc
     2521 ctcgcgacga tggtagctac gttcccgggc gcatgatccg cgcatctgac ctggtggatg
     2581 gactgggcga aagcaacaat ccgcagtgga aaaccgtagc agttaatacc gcaggtgaat
     2641 tggtagtgcc gaacggttcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg
     2701 aatccattgc cgccggtacg gaaaccgaat tgtcgttaac cctgctcggt caacatgacg
     2761 ctgttgcagg cgtggccttc ccctactttg gcggcattga aaatccgcat tttcgcagcg
     2821 taaaacacaa tccggtgctg gtgcgccaat tgcccgttaa aaacctgacg ttagtcgatg
     2881 gcaacacctg tccggtggtc agtgtttatg atttggtact ggcgaattac ggcctcgatc
     2941 gcgggctgga agatgaaaac agcgcgaaag attacgctga aatcaaaccg tacaccccag
     3001 cctggggtga gcaaattacc ggcgtaccgc gccagtatat tgaaaccatc gcccgcgaat
     3061 ttgccgatat cgcccataaa acgcatgggc gctcaatgat tatcctcggc gcaggtgtta
     3121 accactggta tcacatggac atgaactacc gcgggatgat caatatgctg atcttctgcg
     3181 gttgtgtcgg gcaaagcggt ggcggctggg cacactatgt cggccaggaa aaactgcgcc
     3241 cacaaaccgg ctggttgcca ctggcctttg cgctcgactg gaaccgacca ccgcgccaga
     3301 tgaacagcac ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg
     3361 ctcaggagtt actttcaccg ctcgccgatg ccagtaagta cagcggtcat ctgattgatt
     3421 tcaacgttcg cgccgaacgt atgggctggc taccttctgc gccgcagctg gggcgtaacc
     3481 cgctcgggat taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc
     3541 aggcgctgaa atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc
     3601 atccgcgtaa tttgtttgtc tggcgctcta acctgcttgg ctcctccggc aaaggccacg
     3661 agtatatgca gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg
     3721 ccagcgacgg gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc
     3781 tcgacctgct ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg
     3841 ttctgcccac cgccacctgg tatgaaaaag acgatatgaa cacctcggat atgcatccat
     3901 ttattcatcc gctttctgcg gcggtcgatc cggcctggga atcacgcagc gactgggaaa
     3961 tctacaaagg tattgccaaa gcattttcgc aagtgtgcgt gggccatctt ggcaaagaaa
     4021 ccgacgtggt attacaacca ctgctgcatg actctccggc agagctctca cagccgtgtg
     4081 aagtgctcga ctggcgcaaa ggcgaatgcg atctgatccc gggtaaaacc gcgccgaata
     4141 ttgtggcggt ggagcgcgac taccctgcta cgtatgaacg ctttacctcg ctcgggccat
     4201 tgatggacaa acttggcaat ggcggtaaag ggatttcgtg gaatacccag gatgaaatcg
     4261 atttcctcgg caaactcaat tacaccaagc gtgatggccc ggcacagggg cgtccgctga
     4321 ttgacaccgc cattgacgct tcagaagtga ttctggcact ggcaccggaa accaacggtc
     4381 atgttgcagt caaagcgtgg caggcgctgg gcgagatcac cgggcgcgaa catacccatc
     4441 tggcgctgca caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta
     4501 aaattatctc cagccccaca tggtccggtc tggaaagcga tcacgtatcc tataacgcgg
     4561 gatacaccaa cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct
     4621 atcaggatca tccgtggatg cgtgcttttg gtgaaagcct ggtggcttat cgcccgccta
     4681 tcgacacccg tagcgtcagt gagatgcgcc agataccgcc aaacggcttc caggaaaaag
     4741 cacttaactt cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc
     4801 tgctaatgct gacgctctct cgcggtggac cgattgtctg gatcagcgaa acagatgccc
     4861 gagaactgac cattgtcgat aacgactggg tggaagtatt taacgccaac ggcgcgctga
     4921 ctgcccgcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     4981 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     5041 actcggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     5101 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     5161 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     5221 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     5281 ccatacctgt tcggtgacct gtaacaacgt ctggaccgga cgcgaaggca tggagtacgc
     5341 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     5401 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     5461 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     5521 tgattactac gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa
     5581 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     5641 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     5701 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     5761 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     5821 cgctatctac aaacgcgaag aagacggcat tgtgct
//
LOCUS       DAJVPP010000172         5751 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVPP010000172 DAJVPP010000000
VERSION     DAJVPP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.172"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..761
                     /locus_tag="OQM34_003837"
     CDS             <1..761
                     /locus_tag="OQM34_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCS1454074.1"
                     /translation="TGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGV
                     IAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWK
                     QRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAFTCLVGFIIYAVQLAG
                     VPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLTSSLFWIIWFPVIFWM
                     LSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            763..1176
                     /gene="pgaD"
                     /locus_tag="OQM34_003838"
     CDS             763..1176
                     /gene="pgaD"
                     /locus_tag="OQM34_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HCS1454075.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(1226..2014)
                     /gene="phoH"
                     /locus_tag="OQM34_003839"
     CDS             complement(1226..2014)
                     /gene="phoH"
                     /locus_tag="OQM34_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS1454076.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2622..3887)
                     /gene="efeB"
                     /locus_tag="OQM34_003840"
     CDS             complement(2622..3887)
                     /gene="efeB"
                     /locus_tag="OQM34_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS1454077.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(3893..5020)
                     /gene="efeO"
                     /locus_tag="OQM34_003841"
     CDS             complement(3893..5020)
                     /gene="efeO"
                     /locus_tag="OQM34_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS1454078.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5078..>5751)
                     /locus_tag="OQM34_003842"
     CDS             complement(5078..>5751)
                     /locus_tag="OQM34_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCS1454079.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1378 a         1540 c         1384 g         1449 t
ORIGIN      
        1 taaccggtaa tcctcgtatt cgaacacgtt ctaccctggt gggtaaaatt caggttggcg
       61 agtattcctc aattattggt ttgatcaagc gaacccagcg tatctatgga aacgtattta
      121 ccgtttccgg tgttattgcc gcatttcgtc gcagcgccct ggcagaagtg ggttactgga
      181 gtgacgatat gatcaccgaa gatattgata ttagctggaa gctgcagttg aatcagtgga
      241 cgatttttta cgagccacgg gcgctgtgct ggatattaat gcctgaaacg ttaaaagggc
      301 tgtggaaaca gcgcctgcgc tgggctcagg gcggtgcaga agtattcctc aaaaatatga
      361 caaggttgtg gcgcaaagaa aactttcgaa tgtggccgct gttttttgaa tactgcctga
      421 cgacaatatg ggccttcacc tgcctggtcg gtttcattat ttacgcagtc caacttgccg
      481 gtgtaccgtt aaatattgaa ttgacacata tcgctgcgac acatactgcc ggaatattat
      541 tgtgtacgtt atgtttactg caatttattg tcagcctgat gatcgagaat cgctatgagc
      601 ataatctgac ttcatcgctt ttctggatta tttggttccc ggttattttc tggatgctga
      661 gcctggcaac gacattggta tcatttacac gagtcatgtt gatgcctaaa aagcaacgcg
      721 cccgttgggt aagtcctgat cgcgggattc tgagaggtta atatgaacaa tttaattatt
      781 acgacccgac aatcaccagt acgtttactg gttgattatg ttgccacaac catcttgtgg
      841 acattatttg ctttgttcat attcttattc gccatggatc tgctgacggg ttattactgg
      901 caaagcgagg ccagaagccg acttcagttc tattttttgc tggcagtggc gaatgccgtc
      961 gtgttaattg tctgggcgct gtacaataag ctgcgttttc aaaaacagca gcatcatgca
     1021 gcctaccaat atacgccgca agaatatgca gagagcttag caatacctga tgagctctat
     1081 cagcaactac aaaaaagcca caggatgagc gtacacttca ccagccaggg gcaaataaaa
     1141 atggttgttt cagaaaaagc gctagtccgg gcataaacac ccaaaacaaa gcccggttcg
     1201 cccgggctct gcaccgataa tacacttaac tgtatgcatg cagcgtacgt tggcaaagtg
     1261 ccgaacgtac gcaatcctct tttccgaacc ggacgatccc aaccatttca tcttcttcga
     1321 aacgttccag cgcgtcactt aatccggagc acacgccgcg aggcaaatcg cattgcgtga
     1381 tatcaccgtt gacgataacc gtcacgttct ccccgaggcg ggttaaaaac attttcattt
     1441 gcgcggcagt cacattctgc gcctcgtcaa gaatgacaac tgcattttca aaggtacgtc
     1501 cacgcatata ggcgaacggc gcggtttcca ccttccctat ttccggtcgc aggcagtact
     1561 gcataaagga agcccctaag cgccggacca gcaagtcgta gaccgggcga aaatagggag
     1621 caaacttttc tgcgatatca ccaggtaaga agccaagatc ttcatcggct tgcagaactg
     1681 gacgggtgac gataatcctg tcgacatcct tatgtatcag ggcctctgcc gcttttgctg
     1741 cgctgatcca ggtttttccg cacccggctt cgcccgtggc gaatatcagc tgcttactct
     1801 caatagcctg cagatagtgc aattgcgctt catttcgcgc aaggatgggc gaggtatcgc
     1861 gactgtcgcg ggccatacca atggcttcta cgccgctcat ctgcacaagc gaggtgaccg
     1921 attcttcttc acgctgctta tggctgcgcg aatcccgtct cagcacacgt tttgcctcgc
     1981 gacgagcttt gatcactgct ttttgtcttc ccatggagag caccttgagt tgtttgtatt
     2041 catcacacgc gccgttggca gcgcgattat gcgcacgaac atcagagggt tggcttcctt
     2101 gtaagccata gtttgctttt ggataaaatg ccgaaaaacg gctacgcaca ccgtttgcgg
     2161 cgtcggtaac acgagtaaag aaaggaagtg agaaaaatac agagtgacga agttgctacc
     2221 ggaggagaaa tctccgcgag tggtggagcg ttgcttaaca aaacattgtc cagaacagga
     2281 cgttaccatc cgcgatctcc atagtgactg actatcactg ccgggaactt ccgctgctac
     2341 ttaataagta caacagaact cgcattcatt gcaacaatat attcacttat atttaacgat
     2401 aaacacccat ttcggtgaca ttaatttcta ctggaaagat gacagagtga tgacagtgat
     2461 ggaaaaagct gtgtgctttc cgcaggattc gcagggctgg taaaatgtgc ctcgtgtttg
     2521 ccggattcgg cgcgaacgcc ttacccgacc tacaagtgat taataatttg tgcacaaaac
     2581 tcatgcccga taaacctgcg catcgggcat atttgcgtcg ttcaaacctg caatagcgcc
     2641 cttcccagat aatcgttcgc gtccttcact cccggcagcg aaaaaaaata accgccgccg
     2701 ataggtttaa cgtattcctc cagcgcttcg ccattgagcc ttttttgtac tgtcaggaag
     2761 cctttttcca gatcgtgttg gtagcagaca aacagcaacc ccatatccag ttgtccggag
     2821 ttggtgaccc ccaacgaata actgtagccg cgacgcagca tcaggctgga ctcactctcc
     2881 gccgtgcggg gattcgccag ccggatatgg ctgtccagcg cgatcacctt cccttccggg
     2941 tcgctggcgt aatcaggcac atcatgctca tgctgcattc ccagcggcgc accggtttgc
     3001 ttatcacggc caaaaatcgt ctgctgttct ttcagcggcg ttctgtccca aaattccact
     3061 cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc aggctcctgc
     3121 tgatctgccg ttacccacac cactttttgc atcaacttat cattctggct atcgggattg
     3181 gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct ctttgccttt actacgcgcc
     3241 gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacac tgagcaaatc cggcgtgtgt
     3301 ttgatgatat cgcgcagcgc atggttcacc gtgtcctggg tgttggcgca aatctgtagc
     3361 aacacatcac catgacataa cgccgcatcc agcgagtcgt tggggaaacg cgtcatcttc
     3421 tgcagctttt ttggcatctg tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc
     3481 accgataacg tgatggtgag attatcgggc gcaatgtagc cgccaagaat gccggaatcg
     3541 agtggtggca ggcgcggatt tggcgtttct ggtgctgctc cgccctgact cagaaaagca
     3601 aaacgctgag tcaacaagcg aaacaaccgc tcaagatcgg ctttatcgct ggcaagcaca
     3661 tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc ctgatgctca
     3721 ccataaaacg gctgtttctc attgcgtgca tccggtgaaa gcgtgcccgg tgcactttgc
     3781 gttttttgtg catgagcgac cggacaactt cccgccagcg caccaatccc tttcagtaaa
     3841 cgtcggcgtg acggttcatt cacgccgttt tcatctttat actgcataac gcttaatcca
     3901 gtcccagcac accgcgaagt tgcgccagat cttccgccag cgcagtaatc ggccctttca
     3961 gcgcattccg gtcggcatcg gtcaatttgt cgtaggtttc aaaaccgtct ttagtacggt
     4021 atttcgccag aatggtatcg acctttttaa agttggcatc gacttttgcc aacagttccg
     4081 ggttggcttt ttgcagttgt ggacgcagca gatcgacaat tttctgcgag ccttcaacgt
     4141 tagcctggaa atcccacaga tcggtgtggc tgtagcgatc ttcttcaccg ctgattttgc
     4201 tggctgccac ttcctcaatc agtccggctg cgccgccgac cacttttgaa ggtgggaaag
     4261 ccagttcact gatgcgtttt tgcaaatcga ccacatcggt ataaagctgc tcagcgtact
     4321 gatccatccc tttggtggtg ttgtcgccaa acaatgcttt ttccagacgg tggaaaccag
     4381 tgaattttgg gtcggcggct ttttgctcgt aatcatcttc acgggcgtca atgctgccat
     4441 ccagatcgga gaacagttca gcaatcggtt caatacgctc atagtgctgg cgcgtcggcg
     4501 catacagtgc tttcgctttt tcgatatcgc ctgctttaat cgcgtcggta aaggctttgg
     4561 tgtcggtcac cagctgcgtg gtttccgcca tgacatacgc tttatatgca gtaattgcac
     4621 caccaagact taacagcgca tcactttgcg ccgcatccgc cgttgcctca cctttgacga
     4681 tcaacttccc tttcgggtta gtcagcagac cgcaggtcat atcgtattcg ccaggctgta
     4741 aattcgccgt cattttctgg ctaaaaccag gggcgatatt ttcccgctct tccaccacca
     4801 tcacgccttt gaggatctcc cactccagcg ccttctggct atggttctga ataatgaact
     4861 gtgttttccc ggcgttaacc gtaatggtca tcggttcgca ctgcttatcc gtcacggtca
     4921 ctttgacctg cggcacatca gcggcgttag ccataaaagc agaagaaaac agcgcagcca
     4981 cgctcaactg caatgcgtta cggcggaagt taatggtcat gacgagacta tccctttaaa
     5041 gtatgttgta actaagcaag agtttgcgtc gtatttgtta cgcggagcga gacgctgtcg
     5101 cccctgcgcg tggtggcaga gcaaatgcca ccagcgccgg gatgagataa ataaaccaga
     5161 cggcgacttc gctgacgctc ggtgcttcct gatagccaaa aatcccttcc atcagcgtgc
     5221 caaacagcga gtgagttgag aggaccgcgc tcatatcgaa ggcgatttcc tgaaagtggt
     5281 tccacaatcc ggcttcatga aatgcgcgaa tggcaccagc cgccagccct gcggcgacga
     5341 agagaataaa caggctggtc catttaaaaa atgcaccaag attgaggcga ataccgcccc
     5401 agtagagcag gaagccgagc accacggcag tagcaagacc gagcattgca cccagcggcg
     5461 gccagatccc gacatcttgt tgaaatgccg ccagcaggaa aaagaccgac tccagccctt
     5521 cccttgcaac ggcaaaaaag accatcatca ccagcgccca gccatgatga tttccacgct
     5581 gcaatgcgct atcgactgcc tgttccagtt gcactttgac gttgcgcgac actttgcgca
     5641 tccagaaaac catccaggta aggatcacca cggcgatcac cgccacgata ccttcaaaca
     5701 gttcctgctc tttttgcgga aattcgccgg tggtttcgtt aatgaagatc c
//
LOCUS       DAJVPP010000173         5748 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVPP010000173 DAJVPP010000000
VERSION     DAJVPP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.173"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..2085
                     /locus_tag="OQM34_003843"
     CDS             <1..2085
                     /locus_tag="OQM34_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCS1454080.1"
                     /translation="TAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2152..3621
                     /locus_tag="OQM34_003844"
     CDS             2152..3621
                     /locus_tag="OQM34_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1454081.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            3621..5282
                     /locus_tag="OQM34_003845"
     CDS             3621..5282
                     /locus_tag="OQM34_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCS1454082.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFTLPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5503..>5748
                     /gene="symE"
                     /locus_tag="OQM34_003846"
     CDS             5503..>5748
                     /gene="symE"
                     /locus_tag="OQM34_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCS1454083.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELM"
BASE COUNT         1701 a         1362 c         1331 g         1354 t
ORIGIN      
        1 accgcagccg aaggtgaatg ccttgaaaag caaataacgc tggatgactt cccctccccc
       61 gctgaactct ggcaaaaatt ctgcctctgg aaaggataca cacaagctca gcttccggtg
      121 attactcagg actattacga cgatggcagc ggcaaatcgc ctcgttatta tcaacttcag
      181 gcaatcaaca aaaccattga agccgtctcc aacgggcaaa accgcgttct gctggtcatg
      241 gcgaccggaa cagggaaaac ctataccgca ttccagatca tctggcgcct gtggaaatca
      301 aaaaataaaa aacgcatttt gttccttgcc gatcgcaaca ttctggtcga ccaaaccaaa
      361 aataatgatt tccagccatt tggtacggca atgaccaaag tcagcggacg caccattgat
      421 cccgcttatg aaattcacct cgcgctctat caggctataa ctggcccgga ggaagaccaa
      481 aaagcgttta aacaagtcgc accagatttc ttcgatctga tcgtgatcga cgaatgccat
      541 cgcggcagcg catccgaaga cagcgcctgg cgagagatcc ttgattattt cagttccgcc
      601 acccaaattg gcttaaccgc cacgccaaaa gagacgcatg aagtctccag cacggattac
      661 ttcggcgatc cggtttacgt ctactcacta aaagaaggga tcgaagacgg cttcctcgct
      721 ccttataaag ttgtccgtgt tgatattgat gtcgacctgc aaggctggcg cccaaccaaa
      781 gggcaaactg acttaaacgg cgaagtgatt gacgatcgta tctataacca gaaagatttc
      841 gatcgcacga tggtgatcga cgaacgcaca gaactggttg ccagaaccat taccgactac
      901 ctcaagcgca ccaatccgat ggataaaacc atcgtcttct gtaacgacat cgatcatgca
      961 gaacgtatgc gccgcgccct ggttaatctc aacccgcagc aggtgaaaaa gaacgacaaa
     1021 tacgtcatga aaatcaccgg cgatgatgaa attggcaaag ctcagttgga taacttcatc
     1081 aacccgaaaa aaccgtaccc ggttatcgcg accacttcag agctgatgac cactggtgtg
     1141 gatgctaaaa cctgcaaact ggtagtacta gaccagaaca tccagtcgat gaccaaattc
     1201 aagcaaatta tcggtcgtgg tacacgcatc gacgaacgtt acggcaaact ctggtttacc
     1261 atcctcgact ttaaaaaagc caccgaactg tttgccgatg agcgtttcga tggcattccc
     1321 gaaaaagtca tggataccac accagaggat atcgccgatc cagaatctga ttttgaagag
     1381 aaactcgaag aaatcagcga acatgacgac gaacaggtaa caggcgttga tgaaccgcct
     1441 gcgccaccat accaggttac cgataccgat gatgtcggcc catttccgga agaagacgag
     1501 aagaaaatcc gcaagtttca cgtcaacggt gtagcggtgg gcgttattgc acagcgcgtt
     1561 cagtattacg acgccgacgg taaactggtt accgaatcct ttaaagatta cacccgcaaa
     1621 acactgctca aagaatatgc ctcgctggat gactttaccc gcaagtggca ggacgccgat
     1681 cgcaaagaag cgatcattca cgagctggag caacagggga tcatctggga agtactggca
     1741 gaagaagtcg gtaaagatct cgacccgttc gacatgcttt gccacgtagt gtatggtcag
     1801 ccgccgttaa cccgcaaaga gcgcgccgag aacgtgcaca agcggaacta cttcacaaaa
     1861 tactctgaag cagcgcaagc cgtgctcgat aatctgctgg ataaatacgc cgatgcgggc
     1921 gtacaggaga tcgaaagtat tcaggtgctg aaacttaaac cattcgacag catgggcacc
     1981 ttaccggaga ttattaaaac cggatttggc gaccgtaacg ggtataatca ggcgctcagc
     2041 gagctggaaa acgaaatcta ccaattaccg ccccgctctg cttaacaact ctcttttgcg
     2101 ccttctgcaa ccatcatgtt gcagaaggcc ttgtttttca tggaatacaa catgtctatc
     2161 agctcagtaa tcaaatcatt acaagatatt atgcgcaaag atgccggtgt ggacggcgat
     2221 gcgcagcgtc tcggtcagct ctcctggctg ctgtttttga aaatcttcga tgcccaggaa
     2281 gaggcgctgg aactggagca ggataactat caatatccga tcccacagcg ttatttgtgg
     2341 cgcagttggg ccgccaacgc gcagggcatt accggtgatt ccctgctgga attcgttaat
     2401 gatgatctgt ttccggcgtt gaaaaacctc actgcgccta tcgataaaaa cccacgcggc
     2461 tacgtggtga agcaggcgtt cagcgatgcc tataactata tgaaaaacgg tacgctactg
     2521 cgccaggtga tcaacaagct gaacgaaatt gactttacca gcgccagcga acgccatctg
     2581 tttggtgata tttacgaaca gatccttaaa gatctgcaat ctgcgggcaa tgcgggcgaa
     2641 ttctatactc cacgcgccgt cactcgcttt atggtggatc gcgttgatcc gaaactcggc
     2701 gaatccatta tggacccggc ctgcggtacg ggcggttttc ttgcctgcgc gttcgatcat
     2761 gtaaagaaca aatacgtgaa gagcgtcgcc gatcatcaga cactgcaaca acagatccac
     2821 ggtgttgaga aaaaacagct tccgcacctg ctggcgacca ccaatatgct gctgcacggc
     2881 attgaagtac cggtacaaat tcgtcacgac aacaccctga ataaaccgct ttcctcctgg
     2941 gatgagcaac tggatgtcat tgttaccaac ccgccgtttg gtggcacgga agaagacggt
     3001 attgagaaga actttccggc agagatgcaa acccgcgaaa cggcggatct gtttctgcaa
     3061 ctgattgtgg aagtactggc gaaaaacggt cgtgcggcgg tggtattgcc ggatggcaca
     3121 ctatttggcg aaggtgttaa aaccaaaatc aaaaagctac ttaccgaaga gtgcaacctg
     3181 cataccatcg tgcgtttacc gaatggtgtg tttaatccct ataccggcat taaaaccaac
     3241 ctgttgttct ttaccaaagg tcagccaacc aaagagattt ggttctatga acatccgtat
     3301 ccggcgggcg tgaaaaacta cagcaaaacc aagccgatga agtttgaaga gtttcaggcg
     3361 gagatcgact ggtggggtaa cgaggccgat ggttttgcca gccgcgtcga gaatgagcaa
     3421 gcgtggaaag tcagcattga tgacgtgatt gcgcgcaact tcaatctgga tattaaaaac
     3481 ccacatcagg cggaaaccgt cagccatgat ccggacgaac tgttagcgca gtatgcaaaa
     3541 cagcaggcgg agatccagac gctgcgtaat caactgcgcg atattcttgg cgctgcgctg
     3601 tctggcaagg aggttaacta atgagtgtgg aaaagctgat cgttgatcat atggaaacct
     3661 ggacctcggc gttgcaaacc cgttccaccg ccgggcgcgg cagctccggt aagattgatt
     3721 tgtatggtat taagaaattg cgtgagctga ttctggaact ggcggtgcgc ggtaaactgg
     3781 tgccgcagga tccgaatgat gaaccggcgt cggacctgct gaagcgtatt gcggcggaaa
     3841 aagcagagct ggtgaagcaa ggcaaaatta aaaagcaaaa accactgcca gaaattagtg
     3901 aggaagagaa gccgtttgaa ttgccggagg gatgggagtg ggtgcacctc cctgatattt
     3961 attgctcaat ttctgagtca tctagaaaaa taaaatcctc cgaaatacta cctgagggaa
     4021 aatatcctgt tatcgagcaa agtcaagaat ttatttctgg ttattgcaac aatgaatgtc
     4081 tgttaataaa gttaaataat ccagttatag tttttggtga tcacacaagg aatattaaat
     4141 ttattgattt tgattttgtt gtcggcgctg acggtgtgaa gattttatca cctatattaa
     4201 tttgtgagcg tttctttttt tggcagttac gatcatttaa attagatgta agaggatacg
     4261 cgagacattt taaagtatta aatagctgct tatttacatt accccccatt gcagaacaag
     4321 agcgaatagt tgaaaaagtt agctctttga tgtccctctg cgaccaacta gaacagcaat
     4381 ccctgaccag tctggacgca catcagcaac tggttgaaac cctgttggga acgcttacag
     4441 acagccaaaa caccgcggaa ctggctgaaa actgggcgcg tattagcgag catttcgaca
     4501 ccctatttac caccgaagcc agcgtggatg cgttaaaaca gaccattctg caactggccg
     4561 taatgggcaa acttgtgccg caggatccga atgacgaacc cgcctctgaa ctgctcaaac
     4621 gtattgcgca ggaaaaagct caattggtga aagaagggaa aataaaaaaa caaaaaccac
     4681 tgccgccaat tagcgatgag gaaaaaccgt ttgaacttcc ggaagggtgg gaatggtgtc
     4741 gtattggtaa tatagttaat attaaatctg aattagtatc accaaaagat tatttaaatt
     4801 tatatcaagt agcacctgat attattgaaa agggcactgg gagagtgata tcaaagagaa
     4861 cagtgaaaga atcaggagtc aaagggccaa atagtcgctt ttataaagga caaattgttt
     4921 attctaaaat ccgcccgtca ttatccaaag tttttttagc agagtataat ggtctttgca
     4981 gtgctgatat gtatccatta gactgttaca ttaatccaaa ttacttattg aaatatatcc
     5041 tttccattcc atttcttatg caagtcaaaa aagcagagaa cagaataaaa atgccaaaac
     5101 ttaattctga cagtttttac aatattattg ttgcgattcc cccctataat gaacaacaag
     5161 caatatttga taagataaat tctattgaag cagtatgcaa tggtcttatt tcttatattg
     5221 ggatttatca taaaacccaa ctgcatcttg cagacgcact cactgacgca gcgataaact
     5281 aattccctcg ccctcacaaa accaataaag cggctaaata gccgctttat tcacatcacc
     5341 aaaatttata tttcacgcta attttcatct tattttcatt aatttttagc tggactttcc
     5401 ctgtatttac tgatgatgta tccaggtatt tagcacggtg cggatgtgcg ccaacacact
     5461 cgcaccgcta atcacaatca ctatttatag agaatagtaa tcatgactga cacgcattct
     5521 attgcacaac cgttagaagc agaagtctcc ccggcaaata atcgtcaatt aaccgtgagt
     5581 tatgcgagtc gttacccgga ttacagccgt attcccgcca tcaccctgaa aggtcagtgg
     5641 ctggaagccg ccggttttgc caccggcacg gcggtagatg tcaaagtgat ggaaggctgt
     5701 attgtcctca ccgcccaacc cgccgccgcc gaagagagcg aactgatg
//
LOCUS       DAJVPP010000174         5679 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVPP010000174 DAJVPP010000000
VERSION     DAJVPP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.174"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..424)
                     /locus_tag="OQM34_003847"
     CDS             complement(<1..424)
                     /locus_tag="OQM34_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1454084.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEI"
     gene            complement(421..1296)
                     /gene="nanK"
                     /locus_tag="OQM34_003848"
     CDS             complement(421..1296)
                     /gene="nanK"
                     /locus_tag="OQM34_003848"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1454085.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1293..1982)
                     /locus_tag="OQM34_003849"
     CDS             complement(1293..1982)
                     /locus_tag="OQM34_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1454086.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2030..3520)
                     /gene="nanT"
                     /locus_tag="OQM34_003850"
     CDS             complement(2030..3520)
                     /gene="nanT"
                     /locus_tag="OQM34_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1454087.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(3630..4523)
                     /gene="nanA"
                     /locus_tag="OQM34_003851"
     CDS             complement(3630..4523)
                     /gene="nanA"
                     /locus_tag="OQM34_003851"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1454088.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(4645..5436)
                     /gene="nanR"
                     /locus_tag="OQM34_003852"
     CDS             complement(4645..5436)
                     /gene="nanR"
                     /locus_tag="OQM34_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1454089.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1391 a         1575 c         1446 g         1267 t
ORIGIN      
        1 taatctcacc cggctcgcca acaacgcatc ccggcttatg cggctctcct ggcataaaca
       61 cggcgaacat tcccggtttc aggatgatgg tttgctcgtt ttcaatggcg ctacaaagct
      121 ggtaatcatc ttcatggtgg aactcttcac attggcgcgc agtgcctgcc atgccaaaca
      181 gaatccgttc ctcaccgttt aataacagct ggatatcaat gtattgctcg tgcaattccg
      241 cttttttctc gacgggcgat tgagtgttaa acgtcatgac attcataaaa atattgtcgc
      301 cctgtaattc gtaacgaccc ggcgcttttt cttgcggtct ggcagctaag gccagcgtta
      361 acgcgtcctg taacgcagga tgtaacccag cagacggtaa tgactgtact tcacccatca
      421 tcataatttt tctccctggg ccaacagcgc agccccaagt aaacctgcat catggcggta
      481 atgcgccgcc agtaaatcaa catgaaatgc cgctggctcc tgcgccagat acatttccac
      541 caacgccaga tacccttctg ccagaccaac gctgccacca accaccacgc actggcaatc
      601 agttgtggct ttaatatcag cgatcagcct tgcaagcgta cgtgcggagc gatgaatcag
      661 ttgctgcgcc tgctcatcgc cctgcccggc gcgtgtgaaa atagttctcg catccgcgcc
      721 agccagctcg ccctgcgctg ccgccgcaat gccgcgacca gaagcaattg cttccacgca
      781 acctgtgcgt ccacagccgc agactgggcc gtgtggatcg gcaagcgtat gcccgatgtg
      841 ccccgccaga ccgccagggc cggtaagcag tttgccgccg ctcactacac cgccgccgac
      901 gccagtggaa acggtgataa agaccatatc ggttatatcg ccttccagcg cctgatactc
      961 tgcccatgct gcggcctgcg cgtcgttaat ggcaatggtc ggcaaattgg taagttgttc
     1021 cagcgttttg actaacggaa agtgtagcaa tccaccgaga ttatgcggat tcagcgccag
     1081 caaactgccg tcgcgaataa ttccggttga agcgatggca acccgctgcg catgagcttg
     1141 caacggagag actaatgcgg ataaggcatc acgcaaggct tctggtgtct ggctggctgg
     1201 cgtaggaagt tcacgacgat cgcggatctg cccgtcagcg ccaatcagcg cggcggcaag
     1261 tttagtaccg ccgatatcaa tcgccagtat ggtcatagca ccgccttttt catcgctgtg
     1321 ttgtaccact gacaaatgtg ttcaagacgc gtaatggcag aaccgaccgt caccgcccac
     1381 gcgccgtggc gcatcgcatc tgccgcctga gcaggcgtgt tgtaacgccc ttcggcaatc
     1441 acccgacatc cggcttcgct caacgttttc accagcgcca gatccggctc ttctggcgtt
     1501 tcaggcgtgg tatagccaga aagcgtagtg ccaataattt cggctcccag cttttggcat
     1561 gccaggccgt cttccggcgt tgagcagtcg gtcatcgcca gtaaaccgtg atgatggata
     1621 cgtgccagca gcgtttcaac aggcaccgga cgcgggcggt cggtgccgtc aatggcgata
     1681 atgtccgcgc ccgcctgcgc cagcgcatcg acatcttcaa tataggccgt gatgcgtacc
     1741 ggagaatcct ccagatcgcg tttcacaatt ccaataatcg gcacgctcac caccgcacgc
     1801 gtggcttgca gatttgccac accttcgatg cgaatggcaa ccgcgcccgc ctgctctgcc
     1861 gctaatgcca tggctgcgac gatttcgggt ttatcgagcg ggctgtcagg aaccggctgg
     1921 caggagacaa tcaggccacc gttagcagcg attttttgat ccagttgtgc aagtaacgac
     1981 atacatcttc ccttagcgaa aggcccggta catagaccgg gcaacaggat taacttttgg
     2041 ttttgactaa atcgtttttg gcgctgccaa atggcacggc accgctaaat ggtttgccgt
     2101 cgatagcgtc atgagtacgc aatgcttccg ggcgcagcca acgctgaacg cgggaaggca
     2161 tatccagacc aatcagcagg atcaccacaa atgtcaggct gaacgagagc gatgccagcg
     2221 cagtacccag atccagacgt tgagcgatca acgcgccgat gattggggcc agtgcgccac
     2281 ccaatgcgcc aacgttgtag gtaaagccca ggcccgctgc acgctggtcg gtatcgaaat
     2341 aaccgccaat cagtttcggt aagatcccgg cgatcccttg tccaagcatt tgctggaaga
     2401 acagtaacag accgagcacc cagacgtttg cgccgccaat cgcaaatacc ggaataatca
     2461 gcagctgcga ggccagcagg ctacaaacgt acgctttgcg ggttcccagc cagtcaccga
     2521 ggaagccacc tacgcagcat cccacggccg cgccaaagcc actaaagaac agcacattgg
     2581 cgacagtatg cgggttataa gccagatcgg ttttcagata cgttggcagc agcgcctgaa
     2641 tcggccatga gtagaggaaa gcaaacaaca cgaccaccat cagcattacg cccgttggcc
     2701 agcgtttacc cgtactttgc accataaagc tgataaagat tgcggcgcat aacagcccga
     2761 gaacagcgac gattgcagca ttttgcaggt taccggcgaa gcagaaccac agcgcagtag
     2821 ccgccgccag tgtcattacg atattggcaa tgcgatgttc accacggtag agaatatcca
     2881 ccattgtgcg tactggtgct ttacctgcgt gtttctcttt ccagtcttcc gcttccggga
     2941 tgtttttacg cagccagaga gcaaagatga ttggcaaaat gccgataaag aacagcgcac
     3001 gccaacccca gaccggaacc accaggctat agacctgagc ggcaacgacg gcccccacag
     3061 agaagcctga aatcaaaaaa ccactggctt tgttacgcag atgttttggc cagctttcaa
     3121 tgacataggt ggcgctggaa ccgtattcac ccgccatccc catgccgatg accagacgag
     3181 cgataaacat ggtgatgtag cctggcgcaa agccgcaggc cagcgtcccg gccgagaaga
     3241 gaacgatgct ggtgaccatt gccagacgac gcccgtagcg gtcacccata gcgccgagca
     3301 tcaggccgcc gaaccagcga gagataaagg ctgcggagat cagacttgcc gcctgcaccg
     3361 tcgtcagccc gaattcaccc tgtacttcgg tgagtaccag ggcgattaaa acgaaatcaa
     3421 aaccgtcaag cagatatccc aaccaggcag cggaaaatgc gcgccattgt gcacggttga
     3481 gatggcgata ccacgggata ttctgggttg tagtactcat tgtgagtctc ccgcggtggg
     3541 cgatgcccac acgctttggt atgaaaattg tagggtacag atgcgtttat ttcccctcac
     3601 ccggtagggg cgagcgaggg gaaaacaact cacccgcgct cttgcatcaa ctgctgggcc
     3661 agcgccttca gttctggcag atatttttca tctaccggtc caaacggttt gcggcacagc
     3721 ggcacagaaa cgacatccat ataatgcagg acagttttca ggccgcggaa tacgcccgtt
     3781 ttgatcagta aatcaatgac tttgttgcac tcagtttgca gtttctgcgc ggtctggata
     3841 tcgccttctt tcagcgcctt cacgatcccc tgatatcgcc agcccatgat gttgtaagta
     3901 ctgccgatac caccatcagc gcctgccagc aagccggaag cgaagatttc gtcgtaaccg
     3961 ttatagagca ccagatcagg atgttcacga cggatctgtt ccatctgata gagatcgcca
     4021 gaagtctgtt tcagcgcacc gacgccaggc aacgtaacca gggtgttgat ctgatccagg
     4081 gtcagtttta ccccactcag ggctggaatg ttgtacacca ccatcggcaa accatccgct
     4141 gaatcaataa ttgcccgata gtgatcgcag tgttcttcaa agctgaaagg atagtagaac
     4201 ggcgtgacgg cggagacggc atcgaagcca taacgtttag ccgatgccgc aagttgttgg
     4261 ctttcggcgg tgctgacgca accgacgtgg gcgatgagtt taatcttacc tttcgcctct
     4321 tcggcgacga tttccagtac ctgttcacgc tcggaaaggc tttgtacaaa ggcttcgccg
     4381 gtcgaaccac ccacgtataa accgtcgatg ccctgctgaa tattgaactg aaccaggcgg
     4441 cgcagactcg ctttatccag tgcttgttgt tgatcaaaag gagtcaggag tgcagccatt
     4501 acgccacgta aattcgttgc cataaatacc tctgaagtga tgcttgtctg ataaacgata
     4561 tacctttata cctgttatac cagatcaatt aagcaacacc ccatacagaa agcttataat
     4621 gcgatctgct tcactaaagt ggcattattt ctttttgttg gtggtctgac cgaaagcgtg
     4681 ccaggtagca gagacgctgt tgagatgcga ttgcaacgca cgatcggctt cgtcaggatc
     4741 atgacggcgg atcgcatcaa cgatcgcaat atgctgttga taactaacgt tgttatgttc
     4801 gtgcagtgcc tgatcggtaa ccgttgggcg tgcggcaata agccagtcga gcagggcaac
     4861 gtggatcgcc atgaagattg ggttaccggg gatctccgcc agcacgcggt ggaaatcaac
     4921 gtctgaacga atgaatgccg cgttgttatc cagcgactga ctgttgattt ccagtgcttt
     4981 tgccagcaaa tcgatttgct catcggtggc gtgttcagcc gcatagcgca ccaggctgga
     5041 ttcaaagaac agacgtaatt gttcgaaatg ggcaatccca ccaggatgag aaaggaaatc
     5101 tttcgccatg ccggaaagct cgccgatgat agtgtccgca gaaggtcgag agacacgagc
     5161 gcgttcgccg ttgtttattt gcaccagacc tttgcgtttt aacgctgcca gcgcttcacg
     5221 cacagaagga cgcccgacgt taaagaacgc catcagttcg cgttcagacg gtaattgttc
     5281 accttcgcca aattcacgac ggcggatcat ctgttccagc tcttcttcca ccatttcgga
     5341 gagtttttta cgcgccagcg ggcggctacg caagttgcga ccaattgcag gtgaagaatc
     5401 ttcggtttgc gaatcaaatg cgttcataag gcccattctg taaggtcagt gtgattaaca
     5461 tcatcagtga catcctatca caggattgaa agtaggggaa aatggcaggt ttttctcttt
     5521 gtgcctcatc attaccataa ttaacggaat aattaactat tgcgaaaaat taatgtaacg
     5581 cagataaaca catcccgatt gaattattca taagactatt cacaagcatt atgaatatta
     5641 tgaatgtgtt cttacaaaat aatcataatc gcatatttt
//
LOCUS       DAJVPP010000175         5637 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVPP010000175 DAJVPP010000000
VERSION     DAJVPP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.175"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..411
                     /locus_tag="OQM34_003853"
     CDS             <1..411
                     /locus_tag="OQM34_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1454090.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OQM34_003854"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OQM34_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OQM34_003855"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OQM34_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCS1454091.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1391 a         1412 c         1342 g         1492 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc gagaaccacg gcagcctgac ggctgcaaat tgaaaac
//
LOCUS       DAJVPP010000176         5602 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVPP010000176 DAJVPP010000000
VERSION     DAJVPP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.176"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            154..594
                     /locus_tag="OQM34_003856"
     CDS             154..594
                     /locus_tag="OQM34_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1454092.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            658..1887
                     /locus_tag="OQM34_003857"
     CDS             658..1887
                     /locus_tag="OQM34_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1454093.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(1891..2271)
                     /gene="panD"
                     /locus_tag="OQM34_003858"
     CDS             complement(1891..2271)
                     /gene="panD"
                     /locus_tag="OQM34_003858"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1454094.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            2545..3447
                     /gene="rpnC"
                     /locus_tag="OQM34_003859"
     CDS             2545..3447
                     /gene="rpnC"
                     /locus_tag="OQM34_003859"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1454095.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            complement(3521..4372)
                     /gene="panC"
                     /locus_tag="OQM34_003860"
     CDS             complement(3521..4372)
                     /gene="panC"
                     /locus_tag="OQM34_003860"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1454096.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(4384..5178)
                     /gene="panB"
                     /locus_tag="OQM34_003861"
     CDS             complement(4384..5178)
                     /gene="panB"
                     /locus_tag="OQM34_003861"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1454097.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(5291..>5602)
                     /locus_tag="OQM34_003862"
     CDS             complement(5291..>5602)
                     /locus_tag="OQM34_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1454098.1"
                     /translation="TENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNS
                     VYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTY
                     P"
BASE COUNT         1360 a         1343 c         1455 g         1444 t
ORIGIN      
        1 tttgatctgt tggtcgctga tccaacgtgg acgtggtttg cgtagctaag gctatttcct
       61 ctcctctgga ttcgggggag aggagttttg acggctatca ccctttatca acaatggtca
      121 gggtaggctg attttcggct aaggaggaag gcgatgttag gttgggtaat tacctgtcac
      181 gatgaccggg cgcaagagat actggatgcg ctggagaaaa aacatggggc acttcttcag
      241 tgccgggccg tgaatttctg gcgcggatta agctctaata tgctcagccg catgatgtgc
      301 gatgctctgc atgaagcgga ctctggtgag ggtgtcatct tcttaaccga tatagccgga
      361 gcgccaccgt atcgcgtggc ttcattatta agccacaaac actcccgttg cgaagtgatt
      421 tctggtgtca cgttaccgtt aattgaacag atgatggctt gccgtgaaac catgaccagt
      481 tcagagtttc gtgagtgtat tgtcgaactg ggtgggccgg aggtgagtag tctttggcac
      541 caacaacaaa aaaatccgcc tttcgtcctc aaacataatt tgtatgagta ttaacccgcg
      601 attctgatgg cgcttttgct acaataaaag cgttgtttca ccctcggtta ttttttcatg
      661 tataaacaag ctgttattct cctgctgatg ctgtttaccg caagtgtcag tgccgcgtta
      721 cctgcccgtt atatgcaaac catcgaaaat gctgcggtct gggcgcaaat tagtgacaag
      781 atggtgaccg tggggaatat tcgggccgga caaatcattg ccgtggagcc cactgccgca
      841 agttattacg catttaattt tggctttggt aaagggttta tcgataaagg gcatctcgag
      901 ccagttcagg ggcgacaaaa agttgaagac ggtttgggtg acctcaacaa gccgctgagt
      961 aatcagaact taattacctg gaaagatacg ccggtctaca acgcgccgag tgtgggcagt
     1021 gcgccatttg gggtactggc ggacaatttg cgctacccga ttttgcataa actgaaagac
     1081 aggttaaatc aaacctggta tcagatccgt attggcgatc gactggccta tatcagcgcg
     1141 ctggatgccc aacccgataa tggcctgccg gtgctaactt atcaccatat tctgcgcgac
     1201 gaagaaaaca cccgttttcg ccatacttcg acgaccacct cggtacgcgc tttcaataac
     1261 caaatggcct ggctgcgcga caggggatac gcgacactga gcatggctca gctggaaggt
     1321 tacgtgaaga atacgatcaa tctccctgcg cgagcggtgg tgattacctt tgatgatggc
     1381 ctcaagtcgg tgagccgcta tgcgtatcct gtgttgaaac aatatggcat gaaggcgacg
     1441 gtgtttattg ttacctcacg catcaaacgt cacccgcaga agtggaaccc aaaatcgctg
     1501 caatttatga gcgtttctga gcttaacgaa attcgcgatg tttttgattt ccagtcacat
     1561 acccattttt tgcatcgggt agatggttat cgccgaccca tattactgag ccgtagtgag
     1621 cacaatattc tgtttgattt tgcacgttca cgccgcgctc tggcgcaatt taatccacat
     1681 gtctggtatc tttcgtatcc gtttggcggc tttaatgaca aagccgtgaa ggcagcaaac
     1741 gatgccggat ttcacctggc ggtgacaacc atgaaaggca aagtaaaacc gggggataat
     1801 ccgttgttac taaaacgact ttatatctta agaacggatt cgctggagaa gatgtcgcgg
     1861 ctggtgagta accagccgca gggataacaa tcaagcaacc tgtaccggaa tcgctttcgc
     1921 ggtacgtttc atttcattgt cgccttcaaa ataggcgaca ttgggtcgcc aggtgcgagc
     1981 ttcttcatct ggcatggtaa cgaagctggc gatgatgaca atatcgccga cgctggcgca
     2041 gtgggccgcc gcaccgttaa cagaaataat tctcgaaccg cgttctgccg cgatggcata
     2101 agtggagaaa cgcttgccgt tggtgacatt ccagatatcg atggcttcgt tttcgagaat
     2161 accggctgcg tcaagaaaat cctggtcaat ggcgcaagaa ccttcatagt gcaggtccgc
     2221 atgagtcact ttcacgcggt ggagtttgcc ctgcagcatc gtgcgaatca taacttctac
     2281 ctttctaccc tgtcgttaac gaagcaggcg gtgcctgctt tgaggaaatt ctcacgcagt
     2341 attgcccgat ttttgatgag tgtctactca tctgatggca tttgcgtcag cagtttgcgt
     2401 accgcgccga agcgtgccat gtaacgcctg taacgaattg atatttttat gaacgctgct
     2461 cggtaaacaa taaagaggtg gctgacgata gcacgccttg actggatact gccttcacgc
     2521 aaggccacac ggaagacagg gaagatggat gcaccgagta ccacaccgca tgacgcggta
     2581 tttaaacaat ttttaatgca tgcggagacg gctcgcgact ttctggagat acatttgcca
     2641 gtggaattac gcgaactttg tgatctcaac acgcttcatt tagagtcggg gagtttcatt
     2701 gaagagtgcc tgaaaggtca cagcacggac gtgctctatt ccatgcaaat gcagggcaat
     2761 cccggttatc tgcatgttgt gattgaacac caaagcaagc cggataagaa aatggccttt
     2821 cgcatgatgc gttattctat tgctgccatg caccggcatc tggaggctgg ccacgataag
     2881 ctgccgctgg tggtgccgat actgttttat cagggcgagg ccacacctta tccgctctca
     2941 atgtgctggt ttgatatgtt ttactcgccg gagctggcgc gacgcgtcta taacagtcct
     3001 ttcccgctgg tggatatcac catcacaccg gatgacgaaa tcatgcaaca tcggcggatt
     3061 gcgattctcg aactactgca aaaacatatt cgccagcgcg acttaatgtt attgctggag
     3121 caactggtca cgctgattga cgaagggtac actagcggaa gtcagttagt tgccatgcaa
     3181 aactatatgc tgcaacgcgg tcatactgaa caagcggatt tgttttatgg tgtgctgaga
     3241 gacagggaaa cgggagggaa gtctatgatg acgctggcgc agtggtttga agagaaaggg
     3301 attgagaagg ggattcagca gggaagacag gaggaaaggc aagaattcgc cctgcgtctt
     3361 ctaagtaaag gaatgtctcg ggaagacgtt gcagagatgg caaatttacc tcttgctgag
     3421 attgataagg taattaacct tatttaagtt acctgtgtta tgacagatga cgtggggtaa
     3481 attaataact ggcgccatca gccgtagcgc cagttaagta ttacgccagc tcgaccattt
     3541 tgttgtcgat caggcgagca tcgccaagcc aggcggctac cagaattact gcccgtttgc
     3601 tggtttcaga aacttccagc aatgtgtcgg catcgcgaat ctgaatatca tcggcgcgga
     3661 agcctttttc attcagttct tgccccgcaa tggtaataat ttcatcgaga tcccgttccc
     3721 cagcctgcaa tttgtcagca atcgaactta aaactttgta cagaccaggc gcaattttgc
     3781 gttgttccgc cgtcagataa ccgttacggg aacttagcgc cagaccgtct ttggcgcgca
     3841 taattggcac accgacaatc tcaatatcga agcccatatc ggcaaccatt ttgcggatca
     3901 acgccagttg ctgaaaatct ttttcaccga agcaggcgat gtccggctgg accaggttga
     3961 acagcttgct gacaatagtc gaaacgccgc gaaaatgtcc cggacggctg gcaccttcca
     4021 gcatggtcga aaggccagga acgtcaacgt aagtgtgggt ttcagtaccg ttcgggtaga
     4081 tctcttttac cgaaggggcg aaaactaaat ccactttacg tttgtttagc ttctcgcagt
     4141 cctcctgcaa ggtccgtgga taacgtgcca gatcttccgg gcggtcgaac tgcatcgggt
     4201 taacgaaaat actgacgacg accacatcgg cgcgggcttt ggcttcgtcg accagcttca
     4261 tatggccatt gtgcagatta cccatagtag gcaccagcgc cacgcgcttg ccttccatac
     4321 gcaggcggcg aatttgctga cgcagcagcg gcagggtttc gataattaac acaacgtgac
     4381 tccttaatgg aaactgtgtt cttcgcccgg ataaacgccg gactccactt cagccatata
     4441 ctgccgcaca gccgcgcgga tgtcgcccgt ttcggcgagg aaatttttag cgaatttagg
     4501 aatgtgaccg ccggtaatac caaaggcgtc gtgcatcacg aggatttgcc cgtcagtgac
     4561 gttgcctgca ccaataccaa taaccgggat cgccagtgct tcggtaatac gttttgccag
     4621 ctcaaccggc acgcattcca gcaccagcag ctgtgccccc gcggcttcta aggctaatgc
     4681 atcgctgagt agtcgatcgc tcgcttcatt gccgcgcccc tgtactttgt agccaccgaa
     4741 aatattcact gactgtgggg ttaaacctaa gtgaccacat acaggaacgg cacgttcggt
     4801 cagcattttt acggtttcga ccagccactc accgccctca attttgacca tattggcacc
     4861 ggcacgcata accgttgcgg cgttttcaaa ggcttgttcc ggcgtggcat acgccataaa
     4921 cggcaggtca gccaatagca ggcagtttgg tgcgccgcga cgtacggcgg cagtgtggta
     4981 ggcgatatca gcaacggtta ctggcagagt ggaatcgtgg ccctgaacgg tcatgcccag
     5041 cgagtcgcct accagcatga cgttaagccc ttcatcagca aatagtttcg cgaagctgta
     5101 gtcgtaagcg gtgatggtcg cgaaacgctt tttatcctgc ttatatttct gaagtgaagc
     5161 aatggtggtc ggtttcataa cgtatcctga taaatgatgt cgtgttgcct ggcattttat
     5221 cagtcacatt ggtgggggca atgatttatc aattaccgca ccgccagatg atggcggtgc
     5281 ggtggactta ttaagggtag gttacctgga aagtactggt ggctttaaac tcaccagctt
     5341 ctattggtat agttccatcc tgttgtagcg tggcctggaa atggagaggg tatgttatac
     5401 ctgtatcttg atccgggtaa actcctcctg ttgtatcgtc tttgcctctc gggtcgtaat
     5461 ctttataaac agaatttgaa tcattaggtt tcaatgtcat tagaggattt ttactagttg
     5521 ctaaaccttc tatgagtaca ccgactcctt ttgctgcatc attacctgtg agagtattac
     5581 caaggagttg cccgttttca gt
//
LOCUS       DAJVPP010000177         5585 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVPP010000177 DAJVPP010000000
VERSION     DAJVPP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.177"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..245)
                     /locus_tag="OQM34_003863"
     CDS             complement(<1..245)
                     /locus_tag="OQM34_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454099.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSST"
     gene            complement(235..1992)
                     /locus_tag="OQM34_003864"
     CDS             complement(235..1992)
                     /locus_tag="OQM34_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCS1454100.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFYKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2008..2892)
                     /locus_tag="OQM34_003865"
     CDS             complement(2008..2892)
                     /locus_tag="OQM34_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1454101.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2903..3508)
                     /locus_tag="OQM34_003866"
     CDS             complement(2903..3508)
                     /locus_tag="OQM34_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1454102.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3679..>5585)
                     /locus_tag="OQM34_003867"
     CDS             complement(3679..>5585)
                     /locus_tag="OQM34_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCS1454103.1"
                     /translation="ASGLVFIDSYYDNPSTIKGSINARGVFINDIIAPVVASSTNSEF
                     MVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIFKRSNIRMAGNVCNQMKKE
                     ALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMRTLPRLKGISANLRKQLIN
                     SNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDREPLSVEIMTRMSRLPDGVG
                     PLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPESIIDGYIRSDDSSIRQVGA
                     GGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRMTPQESDKVAAYLYQKFEN
                     DDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVIHSDAALQRLLTRFNDPEG
                     WSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETSADMILSELISHGEVDDQM
                     LLNAAALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPRNDIWAKFFLRQENSSRAQ
                     VDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLTFFKFGALSNRHTPSAALA
                     AEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPELDLVRQLALNGKTRAIRE
                     QAMRKLDELY"
BASE COUNT         1621 a         1406 c         1172 g         1386 t
ORIGIN      
        1 gtcgagctac aaacgaacag cgaaaatcca tataaaagat ccagactcca gtaaggaaca
       61 atagtccagg gaagaaaagg gatcgccgtt tcccaaccga atacctgact ggggatatca
      121 tggctgttaa ggtcctgaac cgcggtgaac tgattaagag atccataggt gaagaagaaa
      181 aacggggcca gcaacaataa ccagccagcg ccttgtagca atacgttccg acgttcagtt
      241 atcacgacgc accgccatcg aaaccgtaaa aatgccccac acatcaatcc gttgagtgca
      301 tttataaaat ccggcattac gcacgagcga atccatctcc ccttgcgaac gcacgcgcat
      361 tacccacggt ttaccatctt tatgactggt taacaccccg gcaatcagct ccagttgtgg
      421 gtgccacggc tgcccggtgt agatgagaat gccacccggt tcgatggcat tggctagacc
      481 tgcgagtgag ttttttacct gctcattttc gggaaaaagc tcatataggc cagagacaat
      541 cgccagcgta ggccgcggag ttaacgcgct gagttcctcc gggttaaacg catcgccctg
      601 ttcaaaacgc acccgcccag acattccccg ttgggcaatc atctcttgcc cctgtgcaac
      661 atttaactca ctgtaatcac gtaacaaaat atcgcttacg gcaggctcgt ttgccagcgc
      721 atccagtaca tagcgtccat gccctgcggc aatgtcaacc acgcggacgg ctaaaccttt
      781 ggcgtggaga tcggcaacgg cttgtttaat caatatttgc aaatgggttt tgcgctggcg
      841 aataccgcgc catccaacac tgttcaggta gattttgtcg attaaacgcc cgaatgcgtt
      901 actgccttgc ggttgatttt gatagacata atccagggaa ctgcctgaat caaagccggt
      961 actcattccg agatgcaggc ccgcagagtg cgcaccgaac aatttcatcg cctttcgcat
     1021 aaagcgatac gctaaatcaa ccggcgataa tggcacgggt ccaccagaaa ggagccgcca
     1081 tcgatccgct gatggtcctg tgcggtcttc atgctgataa tcaaattttt gcgatttgtt
     1141 agcatataag cggcagataa agctttgcat tttttcaaat gcctgcgccc tgttctcttc
     1201 acccaatgtg tcgtgataaa agcctggcag cagatgcagc tctttcagag ggctacgtaa
     1261 tctctgataa aaatcaatct gcggttggcg atgcaccaca tagtcatcac ctgatatcag
     1321 aagttgcgtg gggagcgtaa tcgccgccgc atcgctaaca atacgttcag acgttttata
     1381 gagatcgagc aagatattga cggcaatcgc ccgtgtgatc agcggatcat tattgaaact
     1441 cgccacccgt tgccgatcgt gggtcaaata gcgccctttc acataggaat taataaaaaa
     1501 tagaccacgc agacgatgcc ataacgccag cgcaggacgt gccagcggca catacaattt
     1561 aaccttaaag gccggagaag ccagcaccag cccacgaatt gcaggtgcat aatcatgaac
     1621 ccatgtggca acaagcactg cgccgacgct ttgcgcgatc actaccacct cttccagtcc
     1681 gacctggctg tcgctgacag caaaacggac aaattcatcg acatcctgca ctgagcgcat
     1741 aagagacggg ctataaccac gcggccccga attttttcca tgcccccggg catcccaggc
     1801 ataaaaagca gtatcaggca tcgccagttc atcaacgaga tgttgtagac gaccagaatg
     1861 ttcatgcccg cgatgaaata agacgatgac ctttttcgcc cctggctgta aagtgggcca
     1921 gtgccgataa tacaacgctg tattatcact ggtggtaaaa aaatgttccc cagggatgcg
     1981 tgaattttcc attttctcta ttttccttca gtaacagcag tagcgaataa aataaaaaaa
     2041 taccggagcg gtgaaaatta atgagtcaat cctgtcaagg agtccgccat gtcctagtaa
     2101 tagttttcca ctatctttaa caccaatatc tcgtttgatg gctgacatca ctacatcgcc
     2161 gcaaaaacca ctaataccaa ttaacaaacc cgctaataat gcctgtaatg tatttagcgg
     2221 tgtcagcaac ggtccaataa tcagtgacgc aatcatggtg gtgattacgc cccccagcag
     2281 accttccagc gtttttcccg ggctgacttt ggggaccact tttcttctgc cgcaggattt
     2341 cccccataaa tactgtgcaa tatcgtttga ctcggttaac gccaaaagaa ataacaccag
     2401 taacgccccg ccctggatat ttatggttgg caacattaat agccaggcgg catgactcaa
     2461 ggcaaaaacg gtcattatcc atccccaaaa aatggcgctc acggtatgct gaaaaccgga
     2521 ggggtcaccc acaaaaactc gccaggtggc taatatcaga aacccagtca aaggtataaa
     2581 taacagaaac agctcaaagc aattaaaacc gataagcaaa tagttaagag gaataaccca
     2641 atataaccaa cgcgaaaaat gtacggatat aagcgtacaa tattctttca atgccagaaa
     2701 acttatcagc gtaaaaaacg tcaacgtcat ccagcgtggg cctgaaatca ccagtgaaaa
     2761 acaaatgacg atgccccacc aggtacgaat acgtaatgta acttcatccc cttttctctc
     2821 aggtaaccgc catagcagaa aacggtttat cagcttgcta aaattaacaa cgcgaaaagt
     2881 gttgccagag atttttccag cattatcttt cagcgctcat aagaagcaca ctccgacaac
     2941 gattaatcgc agtacagata agcagaattg aagcaatgct ccataaaaga ttattccatt
     3001 gcatccactg tggataaatg gcaacggcca gaccccacag accaaatatt aacgcgcgat
     3061 cacttttgcc aaatggaccg gcataactgc gaacaccgtt aatcgtctgc gcgagtaaac
     3121 cgcaaaactc ggtcaatatg gtgcaaaata acatgagtat cacgagtgat gcgttacttt
     3181 ccggtaaaaa taaaaacggt aaatagagcg caatatcgga aataacatcg ccagtctcat
     3241 tcaaaatcgc ccccagacgt gtttgctgat tgcactcacg cgccaacatg ccatccagcg
     3301 cattgagcgc catacggata aaaagcacga tgggcaatag caaaaagagg atgggttgtg
     3361 ccaccaacac caacagcaat ccggtaaaaa gagaaagcgc cagtgccgta agagtgatgt
     3421 gattcgctgt aacgtggtgc ttataaagcc aaaacatcgt cggccttaac agcgactgaa
     3481 agagcggttt tatctggtag agcgtcatta tcaatccttg ataattcggt ttgcgctatg
     3541 gtgcatcaat tcccctgacg gagatatcgc aacacgccgc ttcattaaaa attcagaata
     3601 tatcaaggta ttgggggctc actgtcagga atgattatcc ccaataaatc aagtagttaa
     3661 aaattattac gctgttattc aatacaactc atcaagtttc ctcatcgctt gttcccgtat
     3721 tgcgcgcgtc ttaccgttaa gcgccagctg gcggactaaa tccagttcag ggttaacaag
     3781 ctctaacgcc agcaaaggat cacgtttcag cctcgccttc aatacatcaa ctggaaagcg
     3841 gggattattc atcgccacaa tccaccactc gggatcaatt tctgccgcca gtgccgcaga
     3901 gggggtgtgg cggttgctaa gtgcgccgaa cttgaaaaat gtgagattgc ttttcgccag
     3961 tgtactccac catatacgat ccggttgttt tgccagtaca tccagttgtg ctaacgcatc
     4021 ggggtcgagt gcataataaa cacgtaatgc ttcatcaacc tgcgcacggg agctattttc
     4081 ctgcctcaga aaaaacttcg cccaaatatc attgcgtggc gtgttttgct gtaattcctt
     4141 gagaacaaca gcgggtaaat tatcccaact aactaatgca ctctctaaaa agtcccagtc
     4201 ttccaaacgt atcagggcgg cggcatttaa tagcatctgg tcatcaactt cgccgtggct
     4261 aatcagctca gacaaaatca tatcagcgct ggtttcatat gcatctgaat ctgctttcgg
     4321 attatttttc cgatgcgata aagcacgttg ccaaattttt tgtttcatcg acgtgctcag
     4381 atactgattt ttcgccagat tactccaccc ctcaggatcg ttgaaacgtg tcaataaccg
     4441 ttgtaaagca gcgtcagagt gaattacctg catatcccga cagcaaaagt aggcagggga
     4501 ttttttctcc atccttttaa caaaattaaa ctgtaagtta tctggcaatg ctaaaaataa
     4561 cacacgtatt agatcatcgt cattctcgaa tttttggtat aaataggctg caactttgtc
     4621 actctcttgt ggcgtcatcc gcaataattg accatggtgt ttcatctctg ccagcttgaa
     4681 cgctaagctt gtgacaacat tgtttgatga atcgttcgcc aactgcattg cctgattgta
     4741 agtgagttgt cctccagcac cgacctgacg aatagaggaa tcatcactgc ggatatagcc
     4801 atcaataatg gattcgggca ataaccacgc atctttataa agctcctgaa tgatcatatc
     4861 ccaatctctt ttcgccagag tcatgacgat atccagaggg agattttcac gtagcgcaag
     4921 attcaacgga ccgacaccat caggtagacg actcattcgc gtcattattt cgacagagag
     4981 cggttcacgg tcgagcaacc ccagaacaac aggctggtaa cccaattgct gatcggtcaa
     5041 ctccagaatc tcattgtcag gcatatatct cgccatactt tcaatcgttt gttcatcatt
     5101 gctattaata agttgtttgc gtaggttagc tgaaattcct ttaagccttg ggagagttcg
     5161 cattatggca agatcaaatg tcgcaatatc tcgtccttca aatgcagcaa cgttgccttc
     5221 atccagcatt tgcaattcct gcactaactc aggagaaggc ttagggcgaa acagcgcttc
     5281 ttttttcatc tgattacaga cattccctgc catgcgaata ttgcttcgct taaaaatttc
     5341 tttcagagca tcttcatcat ttattagacc ccaataatat gcatcaggat ttattatcat
     5401 cagcgctttt ttgacatttt cagtgtcatt tttgtcactc gcacggacca tgaattcact
     5461 atttgtcgaa gacgcaacaa caggtgcaat tatgtcattg ataaataccc cacgcgcatt
     5521 aatactcccc ttaatcgtag acggattatc gtaatatgag tcgataaata ccagtccact
     5581 tgcat
//
LOCUS       DAJVPP010000178         5389 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVPP010000178 DAJVPP010000000
VERSION     DAJVPP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.178"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..649)
                     /gene="rspA"
                     /locus_tag="OQM34_003868"
     CDS             complement(<1..649)
                     /gene="rspA"
                     /locus_tag="OQM34_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCS1454104.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHR"
     gene            complement(855..1181)
                     /locus_tag="OQM34_003869"
     CDS             complement(855..1181)
                     /locus_tag="OQM34_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCS1454105.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1316..1657
                     /locus_tag="OQM34_003870"
     CDS             1316..1657
                     /locus_tag="OQM34_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCS1454106.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1723..2252
                     /gene="speG"
                     /locus_tag="OQM34_003871"
                     /pseudo
     CDS             1723..2252
                     /gene="speG"
                     /locus_tag="OQM34_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2255..2965)
                     /locus_tag="OQM34_003872"
     CDS             complement(2255..2965)
                     /locus_tag="OQM34_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCS1454107.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3073..3378
                     /locus_tag="OQM34_003873"
     CDS             3073..3378
                     /locus_tag="OQM34_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCS1454108.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3577..3900
                     /locus_tag="OQM34_003874"
                     /pseudo
     CDS             3577..3900
                     /locus_tag="OQM34_003874"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4456..5034
                     /locus_tag="OQM34_003875"
                     /pseudo
     CDS             4456..5034
                     /locus_tag="OQM34_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1473 a         1359 c         1250 g         1307 t
ORIGIN      
        1 agcgatggtg catgtcatgc agcaaatgtt cattaaaacc aaacttgtta cgtaccgcgt
       61 caaacaattt cggcatgaaa tcgaggtatt tctccgtcga ccacagctgc tcttccggcc
      121 actgtccttt ggttgcgggt tcataagcca gacctttacc tttcgacatg ccgtaggtgg
      181 ttttcatacc agggattccg cactgcacgc ggatggcttt gaatcccagc tcctgatgac
      241 gggcataatc atccagagct tcatcaatac tgtgaccggt ggtatggcaa taaaccatca
      301 ccccttcacg agacgcgccg ccgagtaact ggtaaagcgg catgttggca gctttggctt
      361 taatatccca cagcgccata tcaaccgctg aaatggccga catcgtaacc ggaccgcgac
      421 gccagtaagc acctttatag aaaaactgcc agatatcttc gatacggtgc gcatcgcgac
      481 caataagccg cggacaaaga tgatcctgca aataagaggc cacggaaagc tcacgtccat
      541 tgagggtggc atccccaagg cccgtaatac cgtcctcagt ggtgattttt aatgtgacga
      601 aattacgccc cggacaggta acaaaaactt cagcctttac gatcttcatg ttcgattcct
      661 tgcatcgctt gtcgtgatgc atgaaatcta cgcaactgag ctactgccat acaagtatga
      721 agatcgaaaa aagccggagt gatcacaaaa aaaggcgtat atttgcgctg tgaatggttg
      781 acaaaagatg aaatagaata ccttttgtca gctgacactt cctcgtatct tattgataaa
      841 atggatttat gttcctacgt gcgcccccag cccgcaacaa tgatcaacat tccgcaaagc
      901 gcaatcaacg cacccgtcca gtcataaaga cttagtttca cgccatccac aacgcgcagc
      961 cacatcaatg ccgtgcagac ataaacgcca ccataagccg cgtaaacacg cccactcgcc
     1021 gcaggatgca acgttaacaa ccagacaaac agcgccagtg aaatccccgc cggaagcaac
     1081 agccagatac tggcgtttcg ttttaaccac aaccagggca gaaagcatcc aataatttca
     1141 cacagcgcag tagcaaaaaa tagtaacgtt gttttaatca tctttgtcac ttattgacat
     1201 catgtatagt tatagggcga cataatatca tcaatataaa catcctcctg gtacgttttg
     1261 cgttcgcagt ggatggtgta gaatcacctt taatcattca tacagggaat gaattatgaa
     1321 aatcactctc agcaaacgaa tcggcctgct cgcttttctg ctgccttgcg cactggcatt
     1381 gagcacaact gttcatgccg aaactaacaa actggtgatt gagtctggcg acagtgcaca
     1441 aagccgccag cacgccgcta tggaaaaaga gcaatggaat gacacgcgca atctgcgcca
     1501 gaaagtgaat aaacgcactg aaaaagagtg ggataaagcc gacgccgctt ttgataaccg
     1561 cgataaatgt gagcaaagcg ccaacatcaa tgcctactgg gagcccaata ctttgcgctg
     1621 cctggaccgt cgaactggcc gcgttattac cccctaacct gttattgatt taaggaatgt
     1681 aaggacacgt tatgccaagc gcccacagtt aagctacgcc cgctggagcg tgaagattta
     1741 cgctatgtac atcaactcga caataacgcc agtgtgatgc gttactggtt tgaggaaccc
     1801 tacgaagcct ttgttgaact ctctgatctg tatgataagc gcatattcac gatcagagcg
     1861 aacggcgctt tgtggtggaa tgtgacggcg aaaaagccgg tctggtggag ctggtggaaa
     1921 ttaaccatgt tcatcgccgg gcagaatttc agataattat ctccccggag tatcagggga
     1981 aaggtctggc aacccgtgcc gccaaattag caatggacta tggcttcacc gttctcaatc
     2041 tctataagct gtatctgatc gtcgataaag agaatgaaaa agcgattcac atttaccgca
     2101 agcttggctt tacggttgaa ggtgaattga tgcatgagtt ttttattaat ggtcaatatc
     2161 gtaatgccat tcgcatgtgt atattccagc atcagtatct ggcagagcac aaaacaccgg
     2221 gtcagactct cctgaagccg accgcacaat agcattaata ataatcgatc gtatttttga
     2281 tggtgtaaac ccgttcgacg gcgggtttta ctccttcatc aacaatgatc agctgacagt
     2341 ccactggatt ggcgtgactg tcatattcac agctctgttt tacattacca actcgttgat
     2401 tatttagtaa agtaaccgct gtgtaatcta attttttgat cggatccgtt gatggcgtgg
     2461 cgctgacaga taatgttttg tcgttacttt tcgtggtttt acccagcgga tacccctgat
     2521 catcatagcg atattccatc tgcatttgtt tgctgctggc tttaatcacg aagccattat
     2581 catcggtttc ccaactcacc ccggcagaag gtaattctgc tagctggcat tttccctgta
     2641 aacgtactct cttctccagc gtctcggcat cacggtaata attggcgtcc agtaccagag
     2701 cgaccacggt attattttcc agatccagta attcgagtga atcaaaacag ccttcttccg
     2761 acaaagtccc agaaacacgt ttcgtcactt caccttgctc atccattaat gtctgagtga
     2821 aatcttttac cggaccacgc agcggatcaa aatcgaattc attagagaaa ctggccatct
     2881 caggggtaaa tgaaagtgtt acctctgtgc ggtcacatcc tgtgaggaat atcgcgagta
     2941 agcatggtag taatttgtat ttcacaacag tcaccagaga gtagagatga ttctcaatca
     3001 tagtagcaaa tacagtactt tacacgttaa atgctatgct taaagaagtt atcttcgcgt
     3061 aaggagctta tgatgaaact ctcaacctgc tgtgccgcgc ttctgctcgc cctcgcctca
     3121 cccgcggtac tcgccgcgcc gggatcttgt gagcgcatac agagcgatat atcacagcgc
     3181 attatcaata atggcgtacc cgaaagcagc ttcacgttaa gtattgtacc caatgaccag
     3241 gttgatcagc ctgattccca ggtcgtcggg cattgcgcta atgatacgca taaaattctc
     3301 tacaaccgca ccaccagcgg taacgtctct gctcccgcgc agtctaccca ggatggcgcg
     3361 cctgccgaac cgcagtaaca cattatcgac tgaacgccgg atatgacaca tccggcgatt
     3421 tgaacataca acataatctc accttattac tcataccctt ctattgatgt ggattaataa
     3481 ttcttaaccc aaaatgggta gactccctct attgttagcg cgctaaatat tcaatatata
     3541 aacatttata taacaataaa gaacagggag tgagttatgt ccaaaaatga ccgaatggtg
     3601 ggcatcagcc gcagaacact cgttaaatca accgcgatag gttctctggc gctggctgca
     3661 ggcggttttt ctttaccgtt caccctgcgc agtgcagcgg cagcggtaca acaggccagc
     3721 gaaaaagtga tctggggttc ctgttccgtc aactgtggta gccgctgtgc acttcgtcta
     3781 catgttaaag ataatgaagt gacctgggtg gaaactgaca ataccggcag cgatgagtac
     3841 ggcaaccatc aggtacgcgc ctgtttgcgc ggtcgctcca tccgccggcg tattaatcat
     3901 cccgatcgct tgaatgaccc aatgaaacgc gtgggtaaac gcggtgaagg caaattcgaa
     3961 cggattagct gggatgaagc cctggatact atcgccagta gcctgaaaaa aaccgtcgaa
     4021 caatatggca atgaagctgt atatattcag tactcttcgg ggattgttgg cggcaatatg
     4081 acccgctctt cgccatcagc ctcggcggtc aaacgcctga tgaactgcta cggcggttca
     4141 ctcaatcagt atggctccta cagcaccgcg caaatttcct gtgccatgcc ctacacctac
     4201 ggcagtaatg atggcaacag caccacggat attgaaaaca gcaagctggt cgtgatgttt
     4261 ggtaacaacc cggcagaaac ccgcatgagc ggtggtggca tcacttatct tcttgaaaaa
     4321 gcgcgcgaga aatcgaacgc caaaatgatt gttatcgatc cgcgatatac cgatacggct
     4381 gccggtcgtg aagaggaatg gctccctatt cgcccgggca ccgatgccgc gctggttgcg
     4441 ggtattgcct gggtattgat taacgaaaat ctcgttgatc aaccttttct cgataaatac
     4501 tgcgtcggct atgacgaaaa aaccttaccc gcagatgcac ccaaaaatgg tcactataaa
     4561 gcctatattc ttggtgaagg tgacgataaa acagcaaaaa cgccgcagtg ggcttcgcaa
     4621 attaccggta tcccggtaga ccgtatcatc aaactggcgc gtgaaattgg cacaacaaaa
     4681 cccgcttata tctgctaggg ctgggggccg caacgccagg caaacggcga gctgactgca
     4741 cgcgctattg ctatgttacc tattttgacg ggcaatgtcg gcatcagcgg cggaaatagt
     4801 ggcgcgcgtg aatcgaccta taccattacg atagaacgcc taccggtgct ggataatccg
     4861 gtcaaaacgt caatctcctg cttcagctgg acggatgcta tcgatcatgg cccgcaaatg
     4921 acggcactcc gcgacggtgt ccggggcaaa gataaactgg atgtgcccat taagttcatc
     4981 tggaacttca aggacttgaa gcggaccctt agtaggcctg aggtcctgca ttgagtcaag
     5041 accttgtgtc attctttgtg ccagacttgt gtcattagac gggatgtcta gacggctggt
     5101 cagccccccc ggtggcctcg gtctggactt gaggtgggcc tatgggggga agtctgctgg
     5161 cttaatgtat tagatggtca ttcacatttt tctgtcagaa accatcggtc ccactgtgag
     5221 tccaaaccta aggacctaga agtccaccca cagccttaga cagagtgtcg aacacatcca
     5281 caccgagtcg accagagagg accatgtaga gacccacaga gaggaacacg agtttataga
     5341 ccagagtcag gagggacttc aggtctgcgg aagtcataga cataaagcc
//
LOCUS       DAJVPP010000179         5375 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVPP010000179 DAJVPP010000000
VERSION     DAJVPP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.179"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            151..1179
                     /gene="torT"
                     /locus_tag="OQM34_003876"
     CDS             151..1179
                     /gene="torT"
                     /locus_tag="OQM34_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS1454109.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEDFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1152..1844)
                     /gene="torR"
                     /locus_tag="OQM34_003877"
     CDS             complement(1152..1844)
                     /gene="torR"
                     /locus_tag="OQM34_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS1454110.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            1941..3113
                     /gene="torC"
                     /locus_tag="OQM34_003878"
     CDS             1941..3113
                     /gene="torC"
                     /locus_tag="OQM34_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS1454111.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3113..>5375
                     /gene="torA"
                     /locus_tag="OQM34_003879"
     CDS             3113..>5375
                     /gene="torA"
                     /locus_tag="OQM34_003879"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCS1454112.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVV"
BASE COUNT         1301 a         1343 c         1491 g         1240 t
ORIGIN      
        1 cgcgtttttc agtctattat ttaatcgtta agtaatctaa tgctatgatt tttaaattat
       61 ttatgtgatt ttgtcatcct ttaggtgaaa aaggttgagt cgcaaagtgg aatgcatcta
      121 gcataaagcc ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc
      181 cttttcatgt tgtcggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat
      241 ttcacggtgc aagcctctat gccgcttaaa gccaaacgcg tatggaaact gtgcgcgctt
      301 tatcccagcc tgaaagattc atactggtta tcgttgaact atggtatgca ggaggctgct
      361 cgccgctacg gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc
      421 cagcaagcac aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt
      481 agcacgacgt catttcccga cctgcaaaag caggtagcaa atctgccggt gatcgaactg
      541 gtaaatgctc ttgatgatcc ccaggtgaaa agccgcgttg gtgtaccctg gtttcagatg
      601 ggctatcaac ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact gaacgtgctg
      661 ctgatgcccg gacccgataa cgccgggggc agtaaggaga tggtcgagga ttttcgcgca
      721 gccattgccg gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa
      781 atccagcgca acctgttgca ggagatgctg gaacgccacc cagaaatcga cgtcgttgcc
      841 ggaacggcca ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt
      901 accgtggtgt cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg
      961 attatggctg ccagcgatca aatggtctgg cagggggaac tggcagttga gcaggccatc
     1021 aggcaattac aggggcaatc ggtgtctgat aatgtcagcc caccgatttt agttctgacg
     1081 ccgaaaaatg ccgaccgaga acatatccgc cgctcgctgt caccgggggg atttcgtccg
     1141 gtctattttt atcagcacac atcagcggct aagaaataac cttcaccatg ttgcgtcacc
     1201 agtaaatccg cgctgagttt atgacgtaaa cgacgaatta acacatcgac ggtgcgcagg
     1261 tcagggtttt ccacccgacg cgcagaaagc atacgcagca gacgctcacg gctgagaatt
     1321 tcgcccggat tagtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc
     1381 ggctcgccat cccgctccag cgtatggcgc gacacattca ggcaataacc cgcaaagcga
     1441 tagcagtagt cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga
     1501 tttttcaccc gtactaccag ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg
     1561 cccatttcca gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc
     1621 cccaccgttg agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggtaag
     1681 ttgatatcca gcagaattaa atctaccggc tgattctgca taatttcccg tagcccggca
     1741 ccgctcgccg taacggaaac ggtatacccc tcctgagtga agtaggattg taatcgcgcc
     1801 tgggtaaccg gcttatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca
     1861 ttctgttcat atctgttcat atctgttcat atttgctcat taagatcgct tcactaaacc
     1921 ataattctac aggggttatt atgcggaaac tctggaacgc gctacgccga cccagtgctc
     1981 gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg ctgattgtat
     2041 tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta
     2101 tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc
     2161 gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac
     2221 tggaagcgag caacgacatt taccagacct ttattgccca ctccattgat acacctgaaa
     2281 aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca
     2341 actcggcaac ctgtcgctcc tgccataact acgatgcgat ggatcatgcc aggcagcatc
     2401 ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc
     2461 ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg
     2521 agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa
     2581 tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga
     2641 aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg
     2701 ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc
     2761 gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc gacaccaata
     2821 ccgagtggag caagttgcag gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata
     2881 tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg
     2941 gcgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga
     3001 ttggctttac cagtctcgat aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga
     3061 atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa
     3121 taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt
     3181 cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc
     3241 gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg
     3301 cgcgatggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc
     3361 gtcgaaaatg atcgccggat tgccggatca cgtacacaac gcggcacgta ttcgttatcc
     3421 gatggtacgc gtggactggc tgcgtaagcg ccatctgagc gacacctccc agcgcggtga
     3481 taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga
     3541 acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac
     3601 ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag
     3661 cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt
     3721 cggttcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa
     3781 aaccattgtg ctgtggggct ccgatttgct gaaaaaccag caagcgaact ggtggtgccc
     3841 ggatcacgat gtttatgaat attacgagca gctaaaagcg aaagtcgccg ccggtgaagt
     3901 tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca
     3961 tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca
     4021 tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt
     4081 tgagcagttc ctgccgtatc tgctgggtga gaaagacggt cagccgaaag atgccgcatg
     4141 ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc ggcagatggc
     4201 ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca
     4261 gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg
     4321 tggttttggc tggcactaca acggcgcagg cacgccgggg cgtaaaggcg ttattctgag
     4381 tggtttctcc ggctctacgt cgattccgcc tgttcacgac aacagtgact acaaaggcta
     4441 cagcagcact attccgattg cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat
     4501 caactggaac ggtaaatcgg taaaactgcc gccgctgaaa atgtgtattt ttgccggaac
     4561 taacccattc catcgccatc agcagatcaa ccgcattatt gaaggctggc gcaagctgga
     4621 aacggttatc gccatagata accagtggac ctcaacctgc cgctttgccg atatcgtgct
     4681 gcctgcgacc acgcagtttg agcgtaacga tctcgaccag tacggcaacc actccaaccg
     4741 tggcattatc gccatgaaac aggtggtgcc gccgcagttc gaggcgcgca acgactttga
     4801 tattttccgc gagctgtgcc gtcgctttaa tcgtgaagaa gcctttaccg aagggctgga
     4861 cgaaatgggc tggctgaaac gcatctggca ggaaggtgta caacaaggca aaggacgcgg
     4921 cgttcatctg ccagcgtttg atgacttctg gaataacaaa gagtacgtcg agtttgacca
     4981 tccgcagatg tttgttcgcc accaggcatt ccgcgaagat ccggatctcg aaccgctggg
     5041 cacgccgagt ggcctgattg agatctactc gaaaaccatc gccgatatga actacgacga
     5101 ttgtcagggg catccgatgt ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc
     5161 gcagaagtat ccgttgcatc tgcaatctgt gcatccggat ttccgacttc actcgcagct
     5221 atgtgagtcg gaaacgctgc gtcagcaata tacggtagcg ggtaaagagc cagtgttcat
     5281 taacccgcag gatgccagcg cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa
     5341 cgctcgcggt caggtgttgg caggagcagt agttt
//
LOCUS       DAJVPP010000180         5213 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVPP010000180 DAJVPP010000000
VERSION     DAJVPP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.181"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            29..244
                     /locus_tag="OQM34_003880"
     CDS             29..244
                     /locus_tag="OQM34_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1454113.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            237..623
                     /locus_tag="OQM34_003881"
     CDS             237..623
                     /locus_tag="OQM34_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCS1454114.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            631..1317
                     /locus_tag="OQM34_003882"
     CDS             631..1317
                     /locus_tag="OQM34_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1454115.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="OQM34_003883"
     CDS             complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="OQM34_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1454116.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2000..3205
                     /gene="tet(B)"
                     /locus_tag="OQM34_003884"
     CDS             2000..3205
                     /gene="tet(B)"
                     /locus_tag="OQM34_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1454117.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="OQM34_003885"
     CDS             complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="OQM34_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCS1454118.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4044..4415
                     /locus_tag="OQM34_003886"
     CDS             4044..4415
                     /locus_tag="OQM34_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1454119.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4425..>5213)
                     /locus_tag="OQM34_003887"
     CDS             complement(4425..>5213)
                     /locus_tag="OQM34_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCS1454120.1"
                     /translation="QFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWLSRVR
                     GKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSRSKGRKNQ
                     RSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEETFRDLKS
                     PAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNV
                     LSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1444 a          958 c         1122 g         1689 t
ORIGIN      
        1 accactatta agtcatgtag ctggttttat ttcaattgaa cgttttcaaa gtctagctac
       61 agaaggaaaa atgttatcgc tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa
      121 aaatcatgtt ttacatgcga aagctcaaca agaagggcga gagtcaatat ttgattttta
      181 caaaattagt attgctcata ttactcgcga atattcattt aaaaaggaca aggataatgt
      241 ttgatgttca cgttgtttta gataatcaaa taggacaatt agcattacta ggaaaaacat
      301 taggtaataa aggtattgga ttggaagggg gagggatatt tacggttggt gatgaatgcc
      361 atgctcattt tcttgttgaa caaggaaagg aagctaaaat agcgctagag caagctggac
      421 tgttagtact tgcgatccgg acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg
      481 aacttggcga aatagcacga gtattggcgg agaataacat taatatttta gtgcaataca
      541 gtgaccatgc taaccaactg atattaataa cggacaatga tagtatggct gcatctgtta
      601 cgctcccttg ggcaataaag tgaacttgcg atggctaatt taatacgaaa agaggttacc
      661 tttgagtcct caatagccgc gataggggcg gctatgtctg acatttcacg agttaaaata
      721 ctcagtgctt tgatggatgg gcgagcttgg acggccactg agctaagttc tgtggcgaat
      781 atatcagctt caacggcgag cagtcattta tctaaattat tagattgcca gctaatcaca
      841 gtagtagctc aaggcaagca tcgttatttt cggctagcag gaaaagatat tgctgaattg
      901 atggaaagta tgatggggat ctccttaaac catggcgtac atgccaaagt ttccacgcca
      961 gtgcatttac gaaaagcacg tacttgctat gatcatttag ctggcgaagt tgccgttaag
     1021 atctatgatt ccctttgtca acagcaatgg atcactgaaa atggttcaat gatcacatta
     1081 agtggtattc aatattttca tgaaatggga attgacgttc cttccaaaca ttcacgtaaa
     1141 atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga
     1201 gccgcattat tttcgcttta tgaatctaaa gggtggttaa ctcgacatct tggttaccgt
     1261 gaagttacca tcacggaaaa aggttatgct gcttttaaga cccactttca catttaagtt
     1321 gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt
     1381 ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg
     1441 tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa
     1501 aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata
     1561 aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat
     1621 gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac
     1681 gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta
     1741 acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg
     1801 taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga
     1861 cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca
     1921 ttaattccta atttttgttg acactctatc attgatagag ttattttacc actccctatc
     1981 agtgatagag aaaagtgaaa tgaatagttc gacaaagatc gcattggtaa ttacgttact
     2041 cgatgccatg gggattggcc ttatcatgcc agtcttgcca acgttattac gtgaatttat
     2101 tgcttcggaa gatatcgcta accactttgg cgtattgctt gcactttatg cgttaatgca
     2161 ggttatcttt gctccttggc ttggaaaaat gtctgaccga tttggtcggc gcccagtgct
     2221 gttgttgtca ttaataggcg catcgctgga ttacttattg ctggcttttt caagtgcgct
     2281 ttggatgctg tatttaggcc gtttgctttc agggatcaca ggagctactg gggctgtcgc
     2341 ggcatcggtc attgccgata ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt
     2401 aggggcaagt tttgggcttg gtttaatagc ggggcctatt attggtggtt ttgcaggaga
     2461 gatttcaccg catagtccct tttttatcgc tgcgttgcta aatattgtcg ctttccttgt
     2521 ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat aatacagata ccgaagtagg
     2581 ggttgagacg caatcgaatt cggtatacat cactttattt aaaacgatgc ccattttgtt
     2641 gattatttat ttttcagcgc aattgatagg ccaaattccc gcaacggtgt gggtgctatt
     2701 taccgaaaat cgttttggat ggaatagcat gatggttggc ttttcattag cgggtcttgg
     2761 tcttttacac tcagtattcc aagcctttgt ggcaggaaga atagccacta aatggggcga
     2821 aaaaacggca gtactgctcg gatttattgc agatagtagt gcatttgcct ttttagcgtt
     2881 tatatctgaa ggttggttag ttttccctgt tttaatttta ttggctggtg gtgggatcgc
     2941 tttacctgca ttacagggag tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt
     3001 acagggatta ttggtgagcc ttaccaatgc aaccggtgtt attggcccat tactgtttgc
     3061 tgttatttat aatcattcac taccaatttg ggatggctgg atttggatta ttggtttagc
     3121 gttttactgt attattatcc tgctatcgat gaccttcatg ttaacccctc aagctcaggg
     3181 gagtaaacag gagacaagtg cttagttatt tcgtcaccaa atgatgttat tccgcgaaat
     3241 ataatgaccc tcttgataac ccaagagggc attttttacg ataaagaaga tttagcttca
     3301 aataaaacct atctatttta tttatctttc aagctcaata aaaagccgcg gtaaatagca
     3361 ataaattggc cttttttatc ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc
     3421 ataaaccagc cattgagtaa gtttttaagc acatcatcat cataagcttt aagttggttc
     3481 tcttggatca atttgctgac aatggcgttt accttaccag taatgtattc aaggctaatt
     3541 ttttcaagtt cattccaacc aatgataggc atcacttctt ggatagggat aaggttttta
     3601 ttattatcaa taatataatc aagataatgt tcaaatatac tttctaaggc agaccaacca
     3661 tttgttaaat cagtttttgt tgtgatgtag gcatcaatca taattaattg ctgcttataa
     3721 caggcactga gtaattgttt tttattttta aagtgatgat aaaaggcacc tttggtcacc
     3781 aacgcttttc ccgagatctc atctattgaa acagcttgat agcctttttc aacaaacaat
     3841 attcgtgctg agttaaccag tgattgatag gtactcttaa aattttcttg ttgatgattt
     3901 ttattttcca tgatagattt aaaataacat accgtcagta tgtttatggt atcatgatga
     3961 tgtggtcgtg acaatcttaa gaacatttag gttattttat gtatattgaa cagcattctc
     4021 gctatcaaaa taaagctaat aacatccaat taagatatga tgataagcag tttcatacaa
     4081 cggttatcaa agatgttcta ttatggattg aacataattt agatcagtct ttactgcttg
     4141 atgatgtggc gaataaagcg ggttatacca agtggtattt tcagcggctg ttcaaaaaag
     4201 taacaggggt cacactggct agctatattc gtgctcgtcg tttgacgaaa gcggctgttg
     4261 agttgaggtt gacgaaaaaa actatccttg agatcgcatt aaaatatcaa tttgattccc
     4321 aacaatcttt tacacgtcga tttaagtaca tttttaaggt tacaccaagt tattatcggc
     4381 gtaataaatt atgggaattg gaggcaatgc actgagagat cccctcataa tttccccaaa
     4441 gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc
     4501 cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt
     4561 acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca
     4621 tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca
     4681 aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc
     4741 aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg
     4801 ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct
     4861 gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg
     4921 cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc
     4981 ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt
     5041 ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag
     5101 taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca
     5161 atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttg
//
LOCUS       DAJVPP010000181         5124 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVPP010000181 DAJVPP010000000
VERSION     DAJVPP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.182"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            199..1107
                     /locus_tag="OQM34_003888"
     CDS             199..1107
                     /locus_tag="OQM34_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCS1454121.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1153..3144)
                     /gene="tkt"
                     /locus_tag="OQM34_003889"
     CDS             complement(1153..3144)
                     /gene="tkt"
                     /locus_tag="OQM34_003889"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1454122.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKQAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3422..4180
                     /gene="loiP"
                     /locus_tag="OQM34_003890"
                     /gene_synonym="yggG"
     CDS             3422..4180
                     /gene="loiP"
                     /locus_tag="OQM34_003890"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1454123.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4386..>5124)
                     /gene="speB"
                     /locus_tag="OQM34_003891"
     CDS             complement(4386..>5124)
                     /gene="speB"
                     /locus_tag="OQM34_003891"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1454124.1"
                     /translation="VSTNLAWEHNRFPWSFDMRERLNVVDCGDLVYAFGDAREMSEKL
                     QAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCEFDH
                     GTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQIVG
                     DMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAY
                     DQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1223 a         1367 c         1348 g         1186 t
ORIGIN      
        1 catcacaaaa tcaacatttt gtttgttatt gtatttgtga tgcatatctc tatttattta
       61 ttgaatgttc gaaaacatac ttttctaatg caatgttttt tcgctatacc cctttataga
      121 ttctgaattc tcacctgtcg gctgttttct cctcatctca tgtgacatat tcccatactt
      181 tactcaagga agattgtcat gaaagcacct gtttcattaa cctcatttcg cccacaaaaa
      241 tctcttgcta tcgccattgg cgtgcttgcc gtggttgtgc tgccatttct ctcgtattac
      301 accgttaatg aaggtgaacg cggcattttg ctgcgctacg gaaaaatcgt aaaagttgcc
      361 gaaccggggc tgggttttaa gatccctttt atggaaagcg tggagaaaat ttccacgcgt
      421 aatcaggctg tggtctatca gggattacag gcctacagcc gcgaccagca accggcacaa
      481 atgacagtat cagtcagctt ccacattaaa ccttcagaag caggcgcggt ttataccacc
      541 tacaacacta ttgaagcatt aaaagatcgt ctaattgtcc gtcagttgcc aactcagctg
      601 gagaacattt tcggccaata taccgccatc agcgccgtac aggatcgtac aaaattggtt
      661 caggatctgc aaaatgccat gcgcaaagcc gttgttgggc cagtggtaat tgacggggtc
      721 cagattgaaa atattgattt ttctgacgct tatgaaaagt cgattgaaaa ccgaatgaaa
      781 gcagaagtcg ccattgccac gcgtaagcaa aatctggaaa cagagaaaat ccaggcgcaa
      841 atagcggtaa ctcaggccca ggcagaagca gacagtaaac tggcggcagc caaagcagaa
      901 gcggaaacta ttcgcgtaag aggcgcggca gaggctgaaa ctattcgttt aaaaagtgcg
      961 gctgaagcag aagcaatccg cctgcgcggt gaagctctgc gggataatcc cggacttgta
     1021 gccctcacca ctgccgaacg ttgggacggt aaactgcccg ataccatgat ccccggcagc
     1081 acagttccgt ttatctctac taagtaaaaa aagggccgct ttcgcggtcc ttctcacaaa
     1141 acgtcttact gattacagca gcgcttttgc tttcgcaaca acgttatcaa cagtgaagcc
     1201 gaactcttca aacagcagct ctgccggagc agattcaccg aaggtggtca tgccgacgat
     1261 agcgccgttc aggccaacat acttgtacca gtagtcagca atacccgctt ctaccgcaac
     1321 gcgtgcagta accgctttcg gcagtacgga ttcacggtaa gccgcatcct gcttgtcaaa
     1381 tgcgtcggta gacggcatgg acaccacgcg cgctttcacg ccttcggcag tcagtttttc
     1441 gtaggcagca accgccagtt caacttcaga accggtggcg atgaagatca gttccggctg
     1501 accagcacag tctttcagca cataaccacc gcgcgcgatg tttgccagct gctcttcagt
     1561 acgttcctgc tgcgccaggt tctgacggga gaggataagc gcagtcgggc cgtcctgacg
     1621 ctcaacgccg tatttccacg cgaccgcgga ttcaacctgg tcacacggac gccatgtaga
     1681 catgttcggg gtcacgcgca gagaagcgac ctgctctacc ggctggtgag tcgggccatc
     1741 ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc tggcgctgtt tcatcagcgc
     1801 agccatacgt acggcgttac gtgcgtattc cacgaacatc aggaaggtgg aggtgtacgg
     1861 caggaagcca ccgtgcaggg agataccgtt agcaatcgcg gtcataccga actcgcgaac
     1921 accgtagtgg atgtagttac ctgcagcatc ttcgttgatt gctttagaac cagaccacag
     1981 ggtcaggtta gacggtgcca ggtcagcaga gccgccgagg aattcaggca acagcgggcc
     2041 gaacgcttcg atagcattct gcgacgcttt acggctggcg attttcgccg gattagcctg
     2101 cagtttagcg ataaactctt tcgctttggc gtcgaagtca gacggcattt cgcctttcat
     2161 acggcgggta aattcagccg cttcctgcgg ataagctttc gcgtaagccg caaacttctc
     2221 attccatgca gattctttcg cctggcctgc ttgtttcgca tcccactgag catagatttc
     2281 agacgggatt tcgaacggtg catatttcca gcccagctgt tcgcgggtca gggcaatttc
     2341 agcgtcgccc agcggcgcac cgtgggagtc gtgagtaccg gctttgttcg gggaaccgaa
     2401 accgatgatg gttttgcaca tcagcaggga cggtttgtca gtcactgcgc gcgcttcttc
     2461 aactgcgcgt ttgatggatg ccgcgtcatg accgtcgatg tcgcgaataa cgtgccagcc
     2521 gtaagcttcg aaacgcattg ctgtgtcgtc ggtgaaccag ccttcaacgt gaccatcgat
     2581 agaaataccg ttgtcatcgt agaacgcaat cagtttaccc agcttcagcg tacccgccag
     2641 agagcaaact tcgtgggaga tgccttccat catgcagccg tcgcccatga aggcgtaggt
     2701 gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc gccgccagcg ttttttctgc
     2761 aatcgccata ccgactgcgt tggcaatacc ctgacccagc ggaccggtgg tggtttccac
     2821 accagcggta taacctactt ccgggtggcc cggagtttta gagtgcagct gacggaagtt
     2881 tttcagttct tccatcggca gatcgtaacc ggtgaggtgc agcaggctgt agatcagcat
     2941 ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca gcccaggacg gattctgcgg
     3001 gttgtgtttc aggaaatcac gccacaggac ttcggcaatg tcagccatac ccataggggc
     3061 acccgggtga ccggatttgg ctttctgtac tgcgtccatg ctcagcgcac gaatagcatt
     3121 ggcaagctct ttacgtgagg acattttgac tccagatcgg atgatgaagg gcacgccctt
     3181 aacgacttga cgacagcgcg ttttgggcta cgccggaaaa tttgccaaca atttaccgca
     3241 agccgcgcgt catgtacatg gaacatcctt ttgccgcttc agaaatctct ggatcatgct
     3301 cgcatgttgc gcaatctgct cgcccgtccg ctgcgctttt ccttatactg agactgagcg
     3361 tcgattcacc tgcaaacggc gcatttttag aataatcctg accttgtgcg gaagagaaaa
     3421 catgaaaatt cgcgccttat tggtagcaat gagcgtggca acggtactga ccggttgcca
     3481 gaatatggac tccaacggac tgctctcatc aggagcggaa gcttttcagg cttacagttt
     3541 gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt caggagatgg acagcaaggc
     3601 gacgattgcg ccagccaata gcgaatacgc taaacgtctg acaactattg ccaatgcgct
     3661 aggcaacaat atcaacggtc agccggtgaa ttacaaagtg tatatggcga aggatgtgaa
     3721 cgcctttgca atggctaacg gctgtatccg cgtctatagc gggctgatgg atatgatgac
     3781 agataacgaa gtcgaagcgg tgatcggtca cgagatgggg cacgtggcgt taggccatgt
     3841 gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg cgagtagctg cggcctctgc
     3901 gggcgggatt gtcggcagtt tatctcaatc acaacttggt gatctgggcg agaaattagt
     3961 caattcgcaa ttctcccagc gccaggaagc agaagccgat gattattctt acgatcttct
     4021 gcaccaacgc ggcatcagcc cggcaggtct tgccaccagc tttgaaaaac tggcgaagct
     4081 ggaagaaggc cgtcaaagct caatgtttga cgaccatcct gcatccgccg aacgtgccca
     4141 gcatattcgc gatcgcatca gcgcggatgg gattaagtaa agcctagtgg tgtcgaacgt
     4201 aggtcagata aggcgttcac gccgcatctg acattaatgg cacatttgtt accttgtgcg
     4261 cattgccgga tgcgatgctg gcgcatctta tccggcctac gggtgccgaa cgtaggtcgg
     4321 ataaggcgtt cacgccgcat ccgacattaa tggcacgttt tacccgtgcg catcgcatct
     4381 ggtgcttact cgcccttttt cgccgcctga atatacagca tctccagcgc cagcgtcgcc
     4441 gcagccagag cggtgatttc cgactgatcg tatgccggag ccacttccac tacgtccatc
     4501 ccaacgatgt tgagatcttt caggccgcgt accagtttaa tggcgcgatc ggaggtcagg
     4561 ccgccaatca ctggcgtacc ggttcccggc gcgaaagcag gatccaggca gtcgatatca
     4621 aaagtcaggt aaaccggcat atcacccaca atctgtttca cctgggcgat aacgtcatcc
     4681 acgctgcgat cgttcacctg gcaggcgtcc agcacggtaa agccgttgtc tttatcaaac
     4741 tcggtacgaa taccaatctg cacggaatga ttcgggtcga tcagaccttc tttcggcgcg
     4801 gtatagaaca tagtgccgtg gtcaaattca caaccgttcg cataggtatc ggtgtgggcg
     4861 tcaaagtgta ccagcgccat tttgccgaaa tgcttcgcat gagcacgcag cagcggcagc
     4921 gtaacaaagt ggtcaccacc gaaggagagc atacgcttac cggcagccag cagcttctcg
     4981 gcgtgcgcct gcagcttttc gctcatctca cgggcatcgc caaaggcata taccagatcg
     5041 ccgcagtcca cgacgttcag acgctcacgc atgtcgaaac tccacgggaa gcggttgtgt
     5101 tcccaggcca gattcgtcga aacc
//
LOCUS       DAJVPP010000182         5024 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVPP010000182 DAJVPP010000000
VERSION     DAJVPP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.183"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..935
                     /gene="tauA"
                     /locus_tag="OQM34_003892"
     CDS             <1..935
                     /gene="tauA"
                     /locus_tag="OQM34_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1454125.1"
                     /translation="AALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVD
                     WRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKK
                     TISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDI
                     DGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSA
                     IDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPV
                     NKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            948..1714
                     /gene="tauB"
                     /locus_tag="OQM34_003893"
                     /pseudo
     CDS             948..1714
                     /gene="tauB"
                     /locus_tag="OQM34_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
     gene            1711..2538
                     /gene="tauC"
                     /locus_tag="OQM34_003894"
     CDS             1711..2538
                     /gene="tauC"
                     /locus_tag="OQM34_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1454126.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2535..3386
                     /gene="tauD"
                     /locus_tag="OQM34_003895"
     CDS             2535..3386
                     /gene="tauD"
                     /locus_tag="OQM34_003895"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS1454127.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3492..4466)
                     /gene="hemB"
                     /locus_tag="OQM34_003896"
     CDS             complement(3492..4466)
                     /gene="hemB"
                     /locus_tag="OQM34_003896"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1454128.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1149 a         1259 c         1425 g         1191 t
ORIGIN      
        1 ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc gtggcgtatc
       61 aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct aaagagagcg
      121 gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg gcgctggctt
      181 ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca gccagccaac
      241 aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa gcgctggtgg
      301 taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc gtaccgttta
      361 tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt aaaccagggc
      421 tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag cggggtgata
      481 ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac ggcaaggtgc
      541 tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc tgggtggtgc
      601 gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa agcgccatcg
      661 atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg gaaaacatca
      721 gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg gtgaagggaa
      781 atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg aacaaagcaa
      841 tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta gcgaatgatt
      901 acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg ctgcaaatct
      961 ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac ctgacgctgg
     1021 aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc accctgctga
     1081 atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg ggtaagcgta
     1141 ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg ctaccgtggc
     1201 gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag aaaatgcagc
     1261 gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca gaaaaacgct
     1321 atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc gcgctggcgg
     1381 cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc ttcacccgcg
     1441 accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag gtgctgttga
     1501 ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg ctttcatccg
     1561 gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt gttgcgggag
     1621 agtcgagccg cagcatcaag tccgatccac aattcatcgc atgcgcgaat atgttttaag
     1681 ccgcgtattt gagcaacgga aggcgttctc atgagtgtgc tcattaatga aaaactgcat
     1741 tcgcggcggc tgaaatggcg ctggccgctc tcgcgtcagg tgaccttaag cattggcacg
     1801 ttagcggttt tactcactgt atggtggacg gtggcggcgc tgcaactgat tagcccgctg
     1861 tttttgccgc cgccgcaaca agtactggca aaactgctca ccattgccgg accgcaggga
     1921 tttatggacg ccacgctgtg gcagcatctg gcagccagcc tgacgcgcat tgtgctggcg
     1981 ctactcgcgg cggtggtgat cggtattccg gtcgggatcg ccatggggct aagcccgacg
     2041 gtgcgcggca ttctggatcc ggtaatcgaa ctttatcgtc cggtgccacc gctggcttat
     2101 ttgccgctga tggtgatctg gtttggtatt ggtgaaacct cgaagatctt actgatctat
     2161 ttagcgattt ttgcgccggt ggcgatgtcg gcgctggcag gggtgaaaag cgcgcagcag
     2221 gttcgcattc gtgccgccca gtcgctgggt gccagccgtg cgcaggtgct gtggtttgtc
     2281 attttgcccg gcgcgctgcc ggaaatcctc accggattac gcattggtct gggtgtgggc
     2341 tggtctacgc tggtagcggc ggagctgatt gccgcgacac gcggtttagg atttatggtt
     2401 cagtcagcgg gtgaatttct cgcgactgac gtggtactgg cggggatcgc ggtgattgcg
     2461 attatcgcct ttcttttaga actgggtctg cgcgcgttac agcgccgtct gacgccctgg
     2521 catggagaag tacaatgagt gaacgtctga gcattacccc gctggggccg tatatcggcg
     2581 cacaaatttc gggtgccgac ctgacgcgcc cgttaagcga taatcagttt gaacagcttt
     2641 accatgcggt gctgcgccat caggtggtat ttctgcgcga tcaagctatt acgccgcagc
     2701 agcaacgcgc gctggcccag cgttttggcg aactgcatat tcaccctgtt taccctcatg
     2761 ccgaaggggt tgacgagatc atcgtgctgg atacccataa cgataatccg ccggataacg
     2821 acaactggca taccgatgtg acatttattg aaacgccacc cgcaggggcg attctggcag
     2881 ctaaagagtt accttcgacc ggcggtgata cgctctggac cagcggtatt gcggcctatg
     2941 aggcgctctc tgttcccttc cgccagctgc tgagctggct gcgtgcggag catgatttcc
     3001 gtaaatcatt cccggaatac aaataccgca aaaccgagga ggaacatcaa cgctggcgcg
     3061 aggcggtcgc gaaaaatcct ccgttgctac atccggtggt gcgaacgcat ccggtgagcg
     3121 gtaaacaggc gctgtttgtg aatgaaggct ttactacgcg aattgtcgat gtcagcgaga
     3181 aagagagcga agccttgtta ggctttttat tcgcccatat cactaaaccg gagtttcagg
     3241 tgcgttggcg ctggcaaccg aatgatattg cgatttggga taaccgcgtg actcagcact
     3301 atgccaatgc cgattacctg ccacagcggc ggattatgca tcgggcgacg atccttgggg
     3361 ataaaccgtt ttatcgggcg gggtgatacg agtgcggacg gttccctcgc ccccttgggg
     3421 agagggttag ggtgaggggg cgttcactgt actttcaaca ggttaactcc ccctttctga
     3481 gaggaaaaaa attaacgcag aatcttcttc tcagccaaat ccagcgcaaa gtagctgaaa
     3541 atcagatccg cacccgcacg cttaattgaa cctaagcttt cgagcacgac tttctcttca
     3601 tctatagcac ccgccagcgc ggcgaactta atcatcgcgt actcaccgct cacctgatac
     3661 gcgccaatcg gcaattcagt acgttcacgc agctcacgca cgatgtcgag gtacgctcca
     3721 gcaggtttaa ccatcaggca gtctgcgccc tgggcttcat ccagcagtga ctcacgaatc
     3781 gcctcgcgac ggttcattgg gttcatctga tagcttttgc ggtcgccttt taatgcgctt
     3841 cctgcagctt cacggaacgg gccataaaag gaagaggcga acttggtcga atacgacata
     3901 atcgccgtat ctttaaagcc tgcagcgtcc agcgcctgac gaatcgcctg tacctggccg
     3961 tccatcgcgg cggaaggggc gatgaagtct gcgcctgcag cagctgcaac cacggcttgc
     4021 ttgcctaaat tttccagagt cgcgtcgttg tcgacgccat gctcgcacag cacaccgcag
     4081 tgaccgtgag aagtgtattc acagaaacaa gtgtctgaca tgacgatcat ttctggcaca
     4141 gtctgcttgc agatgcgcga catacgcgcc accagtccat cttcccgcca ggcgtcgctg
     4201 ccggtttcat cggtatggtg agagatgcca aaagtcatta cggaacggat accggcgttg
     4261 gcgatgcgtt caatttcgcg tgccagatgt ttctctggaa tgcgcatcac gcctggcatg
     4321 gcttcaacgg ctttgtagtc gtcaatttct tcttcaacaa agatcggcaa caccaggtcg
     4381 ttaaggctaa gtgttgtctc ttcaaacata gcgcgcagcg caggagattt gcgcaggcga
     4441 cgagggcgtt ggattaagtc tgtcatggtc tgcctgatgt ttgtggaatt aaggtgcaca
     4501 gtatacctga agcggggcag gagtgtttta cgaaagttgt cgcgatgttg acaagaagag
     4561 aatgaaagag agaccaggac atctttgtaa tagaaatagc aataatttca tattcactga
     4621 aaatattttt aatctttatt tatagctctt ggttattatt ttttggcgtt tggttgtgct
     4681 gctataaatt gaataattca aactttgttg tatattgata agatatttat ctggtttgtg
     4741 tttttgatat ttataacgcg tatgcatcaa aaataacatt atctgcaaca tactgaaata
     4801 tcgtcctttt atcaatttga tgcgtgtttt tctccataaa ttatatatgt ccacattcgg
     4861 acttagggga aagaataatt gaaccattcg tctgtaacgc agcataatcg ttagcgcgaa
     4921 acataatatg ttttctatgc attgataatt gatggatcaa cttattacgt ccctgaggag
     4981 ggatgacaaa tgcactcctg gaaaaagaaa cttgtagtat caca
//
LOCUS       DAJVPP010000183         4980 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVPP010000183 DAJVPP010000000
VERSION     DAJVPP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.184"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(143..301)
                     /locus_tag="OQM34_003897"
     CDS             complement(143..301)
                     /locus_tag="OQM34_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1454129.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            complement(285..596)
                     /locus_tag="OQM34_003898"
     CDS             complement(285..596)
                     /locus_tag="OQM34_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1454130.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(781..1671)
                     /gene="cyoE"
                     /locus_tag="OQM34_003899"
     CDS             complement(781..1671)
                     /gene="cyoE"
                     /locus_tag="OQM34_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS1454131.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(1683..2012)
                     /locus_tag="OQM34_003900"
     CDS             complement(1683..2012)
                     /locus_tag="OQM34_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS1454132.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(2012..2626)
                     /locus_tag="OQM34_003901"
     CDS             complement(2012..2626)
                     /locus_tag="OQM34_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS1454133.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(2616..4606)
                     /gene="cyoB"
                     /locus_tag="OQM34_003902"
                     /pseudo
     CDS             complement(2616..4606)
                     /gene="cyoB"
                     /locus_tag="OQM34_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            complement(4628..>4980)
                     /locus_tag="OQM34_003903"
     CDS             complement(4628..>4980)
                     /locus_tag="OQM34_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCS1454134.1"
                     /translation="PGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPN
                     TMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHE
                     GMEGMDMSHAESAH"
BASE COUNT         1330 a         1328 c         1237 g         1085 t
ORIGIN      
        1 acaacaccaa tttctgaagg ttgtgaaccc tgaaggacgc caagaaacgc gttgtctttc
       61 cagcgaatat gccgtaaaag tacagatgcc aatcagttca ccactttgtc tgtcaataag
      121 tgctttagcc gcacagtcgc tattacgcac acttttcttt agcgatgcgc gtagcgcgta
      181 caaatttatc gataacaggg ttaccgcaat caaactgttt ttgcccagga taagtaatat
      241 cagactgata attacaggag aggactatgg gcagagtaat ctcttcatgc ttgtcgacca
      301 tcgctgtttt tccttttcat taacgccttg agggcgagcg tcggttgagc aggttcagcg
      361 atgagttggt tcatcagttc ccagctttga ttcgaaagct cacggcgacg ttggttatca
      421 agcacacttt cggcgcgttc catagcagcg ctcaaaataa aagcgcttaa atctacccca
      481 acggcttgcg cagcttcacg aagtttgttt ttggcgtcgg gactagtttt gagttcgacc
      541 cgcgcatttt tcagcgtttt atcgacatcg tatagagcgg ctgtttttcc aggcattatg
      601 acttctttga ttaactgaat gaaatcgtag ggagtcgatc tcatacataa tacggctatc
      661 aaccgtatga ttaatttatg tgcatctgct cactctgtca agagatacgg ttattagccg
      721 tatctctgat ctcagacaat aaaaaaggtg ccgtagcacc tttttaatag agaggttttg
      781 ttaccacaca gcagccagca gcgtatgcga gtccggtacc ataaaatcaa cggacatcat
      841 caccgagagg gcagtgatgg cgatgataga gaagccgaac agcttgcgcg cccagattct
      901 gtcatcagca actttataac cgcgcagagc catacctaac caccagacgc taaccgccgc
      961 ggcgaccacc agatatttat acccagcgta accgccaaga gagagcatca gcgtggcaac
     1021 ggcaaaggcg atgatataca gcgtgatgtg attcttcgcc accgaaatgc cttttaccac
     1081 tggcaatacc ggaatgtttg ccgcctggta atccttaaag cggaaaatgg cgatggcata
     1141 ggagtgaggc atctgccaca ggctgaagat agccagcagg atcgctgcgc cgctatcaaa
     1201 ctcgccggtt actgcacagt agccaatcac cgacggcgca gcgccggaga gcgaaccaat
     1261 caatgtgccg tagactgagt ggcgtttcat gtacaggcta tagacgccga cataaaccac
     1321 aaagcccatc acccccagcc agcaggccag cggattcgcg ccaaaccaca gcagcataaa
     1381 gccagcaata cccagcaagg tggcgtacac cagcgagaca gcaggagaga tcaggccttt
     1441 aaccagcacc cgattcttcg tcctttccat ctttctgtcg atatccctgt cgatgtagtt
     1501 gttaaacaca caacccgacg ccacaaccag tgacacccca accagcgtgt agataaacag
     1561 gggataatca atgctgccct ttgaggccag caggaatccc ccaatcaccg agatcaggtt
     1621 gccaaagatg atgcctggtt tcgttacttg caggtattgc ttaaacatca taaccgccgc
     1681 tcttagtgca tcatcatgtt gtagttgagg ttccacataa tccagatgga gcctacaacc
     1741 aggatagcga tgattagcac ggtgaagaca aacgccgtca tgttccagcc ttcatctgat
     1801 ttggtattca tgtgcaggaa gcacaccaga tgcaccagaa cctgtaccac tgccattgcc
     1861 aggattgttc ccagaattac ggccggagag gcagcccctg tcatcaccat ccagaacgga
     1921 atcaccgtca gaatgatcga caggataaag cctgtcatgt aggtttttac gctgccatgg
     1981 gacgcgccgc tgtgatcggt agaatgactc attacatcgc ccccatcaga taaacaacag
     2041 tgaacacaca gatccaaacc acatccagga agtgccagaa caggctcagg cacatgatgc
     2101 gggtacggtt agtgctggtc aggccgcgac gggcgatttg caccatcagc accgccatcc
     2161 agataagacc agaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag aacgctgaca
     2221 ggaagccgct gcgatccggc cccatgccgt taacaatcag gtgatggaat tcatagattt
     2281 ccatcccgat aaatccggca ccaaacaacc aggtcaacgc cagccaggag ataacctggc
     2341 ttttgttgtt tttgtacatg gcgatagccg ccatgccgta ggtgatggag ctgaacaaca
     2401 gcaagaaagt ttcaaccaga acgaacggca gttcgaaaat gtccttacct gtcgggccgc
     2461 ctgcggtgcc gttcaccaga acggcatagg tagcaaacaa gattgagaac agaatgcagt
     2521 cgctcatcag gtagatccaa aatccgaaga ttttggttcc gcctgcatcg tggtgcccgt
     2581 gttcgtgcgc gtgggcagtc gcgtgcgtca aagtatcagt tgccattttt cagccctgcc
     2641 ttagtaatct catcgaaatg ctggttttcc agtttttcga tttctgccac cggcacgtag
     2701 taatccacgt cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc tgcgaagcca
     2761 acaatcgcca gccaccagat atgccagatc atggcgaaac cgaagatggt ggagaaagct
     2821 gcaatgacga tacctgcacc gctgtttttc ggcatatgaa tttcttcata gtggtcaggc
     2881 tttttatacg cttcgccttt ctctttcatt tcccagaatg catcacgttc gtgaacgtgc
     2941 ggcactacgg caaagttata gaacggaggc ggggaagagg ttgcccactc cagcgtacgg
     3001 ccaccccacg ggtcgccagt caggtcacgg ttctggtcgc ggtcgcgaat agaaacgtac
     3061 atctgaataa cgaggcagag aatacccagc gcaatcagta ctgcaccgct ggctgcaatc
     3121 atcagcatgg tgtggaactg cgggtcaatc tgctggctca aacgacgggt catgcccatg
     3181 aagcccagcg catacagcgg cataaaggca acgaagaagc cgatgatcca gaaccagaac
     3241 gcgcgtttac cccaggtttc gttcagtttg aaaccgaacg ctttaggcca ccagtaggtc
     3301 atccctgcga agcagccgaa gaccacgccg ccgatgatca cgttatggaa gtgcgcaatc
     3361 aggaacaggc tgttatgcag aacgaagtcc gcgcccggta ctgccagcag cacgccagtc
     3421 ataccgccca ccgagaggtg acgataaaac cgatagtcca cagcatcgca gaatggaaca
     3481 cgatgcggcc ctgatacatg gtgaacagcc agttgaagat cttcaccccg gtcgggatgg
     3541 cgataatcat tgtggtgata ccaaagaagg cgtttacgtt cgcgcccgca cccatcgtaa
     3601 agaagtggtg cagccaaacg atgaacgaca gcacggtgat gcagacggtt gcccatacca
     3661 gcgaggtata accaaacaga cgtttacgcg agaaggttgc cgcaatttcg gagaacacac
     3721 cgaaaacagg caggatcagg atgtaaactt ccgggtggcc ccaggcccaa atcaggttga
     3781 tgtacatcat catgttgcca cccatatcgt tggtaaagaa atgggtgccc agatagcgat
     3841 ccagggtcaa caacgcgacg gtaaccgtca gaattgggaa ggaagcaata atcagtacgt
     3901 tcgcgcacag tgatgcctag gtaaatactg gtatcttgaa catggtcatg cccggtgcgc
     3961 gcatcttcag aatggtaacg aagaagttga taccggtaag cgtcgtacct ataccggata
     4021 gctggagact ccatatccag taatcaacac cgactcccgg actgtactct attcccgata
     4081 gcggtggata ggccagccag ccggtctgcg caaattcgcc cacgccgaga gaaacgttaa
     4141 ccagaatcac gccaacaaca gtaaaccaga agcttaagtt gttgaggaac gggaacgcaa
     4201 cgtcacgcgc gccgatctgc agcggaacca ccaggttcat cagaccgata acgaaaggca
     4261 tcgccacgaa gaagatcata atcacgccgt gcgcggtgaa gatctgatcg tagtggtgag
     4321 gtggcaggaa gcccgcttca cccgccgagg caagagcctg ctggctacgc atcataatgg
     4381 cgtcagcaaa accacgcagc aacatcacaa tcgccacgat gatatacatg ataccgaggc
     4441 gtttatggtc gacggaggtc agccactctt tccacaggta ggtccactta ccgaagtaag
     4501 tgatcaggcc aacgagcgcc agacctccca aaataatgcc agcgatcgta accatgacga
     4561 tagtttcatg gaacgggact gcatcaagtg ataattttcc gaacatcttt attcttcctc
     4621 aaccccttta atgggcggat tccgcgtggc tcatgtccat gccttccata ccttcgtgtg
     4681 cactgtgctc accttctggc tgggtcatgt ccatgctctt accgtgagcc ataaacttgt
     4741 taatcacatc ggcaaacaag tctggtttca cgttggagaa atattccacc tggttgtatt
     4801 cgctaggcgc ggccagtttt tcgaacgcag ccatgtcaga catggtgttc ggcgactgtt
     4861 tcgcttttgc gacccattgg tcgaattcgg cgcgatccgg tgttgcaata gctttgaact
     4921 tcatgcctga gaagcccggc ccgctgtagc tggcggagat accgtcataa gtaccgggtt
//
LOCUS       DAJVPP010000184         4894 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVPP010000184 DAJVPP010000000
VERSION     DAJVPP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.185"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..422)
                     /locus_tag="OQM34_003904"
     CDS             complement(<1..422)
                     /locus_tag="OQM34_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCS1454135.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPR"
     gene            complement(433..2730)
                     /gene="xdhA"
                     /locus_tag="OQM34_003905"
     CDS             complement(433..2730)
                     /gene="xdhA"
                     /locus_tag="OQM34_003905"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS1454136.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            3145..3900
                     /locus_tag="OQM34_003906"
     CDS             3145..3900
                     /locus_tag="OQM34_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1454137.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3979..4052
                     /locus_tag="OQM34_003907"
     tRNA            3979..4052
                     /locus_tag="OQM34_003907"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4011..4013,aa:Gly,seq:ccc)
     gene            4274..4819
                     /locus_tag="OQM34_003908"
                     /pseudo
     CDS             4274..4819
                     /locus_tag="OQM34_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1261 a         1174 c         1242 g         1217 t
ORIGIN      
        1 cgcggggagt ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg
       61 ctggtggcac cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca
      121 gcaatgaacg tggccgcagc acataacgcc gggagatgac gttgagttat aggatcttct
      181 attagctggg taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta
      241 attccccgca gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga
      301 tggtggagct gaatcagtac gtcagtgcca ccggcgagca gtttggcctg cgggttgtca
      361 gccagcaggt tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac
      421 atgatgttat cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt
      481 cagcggcagt gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc
      541 ggcaacagga attatgggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc
      601 attgatttcg acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta
      661 atccagcaga ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa
      721 tagcgcccag ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg
      781 attaagaata tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag
      841 cgcaatatcg accgtcagat caacaaaggt acagccaaac gccggtgggt tagtggtggt
      901 tttgatggag ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat
      961 cgccaaatct tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc
     1021 ttttatcagg gtcaaattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat
     1081 tttctctttt aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg
     1141 tgaggcaaat gcgccgggat cgaacggcgt aacgtcggta tcttgtgttg aaataacgcg
     1201 aacgtcgctg accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc
     1261 accctgaccg atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag
     1321 aaggcgcgcg cctgctattt ctacgccgac aggccaggtg ttagaggtgt agctaaaaca
     1381 ggcgacgcca acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt
     1441 ttcccattca aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgcaaat
     1501 acgtttgccc gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc
     1561 aacaggatca atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac
     1621 gacttgtggc gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt
     1681 ctttgaactg taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcccccccgc
     1741 agaagcgatg gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact
     1801 ataacctttc aaggttccgt cgcggttcac gcccatttgc ccgtcaatag taaaagcgtg
     1861 gcgggtacgg gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc
     1921 gccaagcttg ctggtcagga atgccgccat tggctcttcc agtacatcct gtttattacc
     1981 aaaaccgcca ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc
     2041 ctgaccaacc acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg
     2101 cgagtcatcc tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac
     2161 gggagtctga tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt
     2221 gcccgtcgac atcgtgcttt gtttcagtaa attgccgccg ttatggattg gtgcagcgtc
     2281 ttctgtcagc gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac
     2341 caattgcgcc gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc
     2401 accatgatga cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc
     2461 gtcaagtgtc caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaagt
     2521 aaaaatcgcc agtacgcccg gtaaacttct ggcttgttca tcattaatac ttacggcata
     2581 accatgtgcg atagggctac gtacatattt cgcgtaacac atgcccgcca taacataatc
     2641 gtcagtatat cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc
     2701 acccgtagcg gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc
     2761 ttatggcaaa gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta
     2821 ggggaggaag gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct
     2881 gaatagggac agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaatcagg
     2941 attattacgc atcaggatgg gaaatagggt aaaaagtcag gaacttcgat acctctacag
     3001 cgaatatttt gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac
     3061 cacgtttttc cagaagcaag agattgggta tcaagaggct ggctgctatg atatggcgtc
     3121 ttgtttttta agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt
     3181 atcgtgatgt ttttatcggt tggactgcta ttggcgggct gttcgggtag caaatcatcc
     3241 gatacaggaa cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt
     3301 atttcgcgca ccacgggaac cagcgtaaaa gagctggcgc gactgaacgg catttccccc
     3361 ccttacacca ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt
     3421 acacgtaaat caaccgccaa atcaacgact aaaaccgcat cggttacacc gtcatcagcg
     3481 gtaccgaaat cttcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg
     3541 aaagttatca tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct
     3601 ccacggggta cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag
     3661 ctgcgtggct acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac
     3721 gcccataatg acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc
     3781 gccactatgg ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt
     3841 gcaacggcaa ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga
     3901 tggcgaatta atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa
     3961 tgccttacgc atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct
     4021 atacgagggt tcgattccct tcgcccgctc caatttatct tcacctgatc aatctgccat
     4081 ttttgctgta tctgattttc tatattatct taattcaatc acttcaggct tttcgcaatc
     4141 gctgtaacac ttccccattt ttttgtaaat aaaaagcgtt tacgcaatgc aatccttttg
     4201 taacaaccca ggtaaaatac cttattttca agttgttgaa taagtggtta tctacgcttt
     4261 taacaaacga ttgatggatt attttatgaa agggaaatct gcattaactc ttctgctcgc
     4321 tggtatattt tcgtgcggca catgccaggc gacagaggca gaagtgacaa gcgaatctgt
     4381 atttaacatt cttaattcca ggggggcagc aacagataaa agttatttat cgttaaatcc
     4441 agataagtat cctaattatc gtcttctgat tcactcagcc aaattaaaaa atgaaataaa
     4501 gagtcactat accaaagacg aaactcaagg attgctaaca ttaacagaaa acacacgaaa
     4561 gttaacgttg acggagaaac cctggggaac gtttattctt gcttctactt tcgaagatga
     4621 caaaacagcg gcagaaaccc actatgatgc tgtctggctg agggatagtt tatggggcta
     4681 tatggcatta gtatcagacc aaggaaatag tgtagccgca aaaaaggttc tgctcaccct
     4741 gtggggttat atgtctacgc ccgaccagat taagcggatg caggacatca ttagcaaccc
     4801 aaaacgactg gatggaattc tgatcctacc cacgtaatat ggacacaggc ctaagcgagg
     4861 ttctggtttt caaattgttc cggactgagg ccgc
//
LOCUS       DAJVPP010000185         4816 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVPP010000185 DAJVPP010000000
VERSION     DAJVPP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.188"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..118
                     /locus_tag="OQM34_003909"
                     /pseudo
     CDS             1..118
                     /locus_tag="OQM34_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(168..1118)
                     /gene="uspE"
                     /locus_tag="OQM34_003910"
     CDS             complement(168..1118)
                     /gene="uspE"
                     /locus_tag="OQM34_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1454138.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1270..2022)
                     /gene="fnr"
                     /locus_tag="OQM34_003911"
     CDS             complement(1270..2022)
                     /gene="fnr"
                     /locus_tag="OQM34_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1454139.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2217..2732)
                     /gene="ogt"
                     /locus_tag="OQM34_003912"
     CDS             complement(2217..2732)
                     /gene="ogt"
                     /locus_tag="OQM34_003912"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1454140.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(2743..2886)
                     /locus_tag="OQM34_003913"
                     /pseudo
     CDS             complement(2743..2886)
                     /locus_tag="OQM34_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3276..4091)
                     /locus_tag="OQM34_003914"
     CDS             complement(3276..4091)
                     /locus_tag="OQM34_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1454141.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4188..>4816
                     /locus_tag="OQM34_003915"
     CDS             4188..>4816
                     /locus_tag="OQM34_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1454142.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLE"
BASE COUNT         1278 a         1114 c         1144 g         1280 t
ORIGIN      
        1 gtggacgaca tcaatgtatg tgatttagtc atcgatgatt tttgtttata gcctggtctt
       61 tactgtgttg ctggcaatcc cgttgggaat ttatttcctt ggcggcgaag agcagtaagt
      121 aaaaaatagg cccgataact cgggccttgt cagttattga agagtcgtta atcgtcttct
      181 tcgtcatcca gttcaacggg tgtctgatac tggtcaggtt taatgaccag caggtcgcag
      241 cgaagatgat caatcacctg ttccgccgtg ttgccgagga atgctgctga aataccggtg
      301 cgtccgaccg tgcccagaac cacaattccc gcctgtaaat gctccgccaa atcaggaatc
      361 acctcttcgg gcagaccttt ttctacgtgc gtcatgtttt cattaatgcc gaatttctgc
      421 cgcagggctt tcattgccag caaatgttgc ccacgaatgg catcgttata aacgctcggg
      481 tcaaattccg gcagttcaat cgcgatatta attggcgtta ccggataagc gccaaccaga
      541 tgaacttcgg tatggttgac ttgttctgcc agttcgatcg tctctttgac cagtttttca
      601 ttgagcgcat tatgatacgg ctcttcactg gcgagattca ccgccaccag cgccttgcct
      661 ccttccggcc acggctggtc tttcaccatc cacaccgggc ttgggcattt gcgtaacaga
      721 tgccagtccg ttggcgtaaa aatcaccgct tccagacggt catgttggtg cgccattttt
      781 agcaccaaat cgtgtccgcc gctgatcact tcctgaatga tggcttcgaa aggacggtta
      841 tgccagacca ctttaatttc aatgggaacg ccagcattga gataatattt tgcctgctcg
      901 tggatccagg ctgtacgctg gctgatgacg ccctgacgca tagcggtacg ttcgtccggg
      961 gagagcaggg tggtcatttc gtatgagaag tcatagatcg gcaaaaaggc tttaattttg
     1021 ccaccaatcc gttgatgtaa ataaacagct cgccgcaatg ctggttggtc gtcctggtta
     1081 ggatcgataa caacgagcat gttctgatac atagccatac agggtctcct tacaacaact
     1141 gtcaacgcag tttgtaatta aaagattaac ccatatctgg tgaatgaaac agtgatgaac
     1201 cttctgccag atcaataaat cagaaaaatt taatgatatg acagaaggat agtgagttat
     1261 gcggaaaaat caggcaacgt tacgcgtatg accagcaagc tgggccagcg catcgttatt
     1321 ttcgatggtg atgtatttac ctttgactgc cagcataccg cttttctgga agcgacccag
     1381 cagacggctg atggtttcta ccgtcaggcc cagatagtta ccaatatcgc cacgagtcat
     1441 cgtcaggcgg aattcacgag gggagaagcc gcgttgggca aaacgacggg acaggttgta
     1501 gatgaatgca gccagacgtt cctcggcatt tttcttcgac aacagcaaga tcatgtcctg
     1561 atcgcctttg atttcaccgc tcatcagacg catcatctgc tgacgcagat tcggcatttt
     1621 accggacaaa tcgtccagcg tttcgaacgg aatttcacat accatcgagg tttccagcgc
     1681 ctgagcgaag ctcgggtgat ggccgctgcc gatggcgtca aatcccacta ggtcgcctgc
     1741 taaatggaaa ccagtgattt gctcgtcgcc ttgctcagtg attgtataac ttttaatcgt
     1801 accggagcgg atggcataaa gcgatttaag ttcatcacca gccttaaaca gcgtctggcc
     1861 tttctgaata ggcttcttcc gctcaatgat attatcaagc tgatcaagct catgttcgtt
     1921 gagtgtgaac gggatgcaaa gctggctgat gctgcaatcc tggcaatgga tagcacaacc
     1981 gccagactga atgcgccgta taattcgctt ttccgggatc ataggtctgc tcaagccgta
     2041 attgatattt gtcaatttta acatcttttt agggagcaag taagtctaag caaaccttaa
     2101 cagcagagaa ttccgatatt agatgtaaat atatgtctat ctatttgaaa ctccttaagt
     2161 tgtcaagggt aactttacat aaaagtgtga acaagctggc acaaattgtt taatgtttac
     2221 agcaaaagat aaccttcgtg gcgcaataac cactcttttc gctgaactcc gcctgcatat
     2281 ccggtcatgg tgccgtttcg gccaataacc cgatggcaag gtacgacgat gctgatggga
     2341 ttcgatccgt ttgccgcacc aacggcacgc gccgcgccag ggcggcccaa ttgttcagcc
     2401 agttggccgt aatgcattac ctgcccgcag gggatagtgc gtagtgtttt ccagacttcg
     2461 cgctgaaatg gcgtcccccc cgtggcagtg ggaagcgtat caataatgct aagattaccg
     2521 gcaaaatatt cacgaagctt gtcgcttaaa ccacctgggt tggtggcaga aatgcgctca
     2581 tagccttctt tgcgataatg gatgtccagc agctgcacca tgcgttcgct gtactcttcc
     2641 cattcaaccg cccgcaggcg aaattgctca tcgcaaatca cccacagtgg acccagtggc
     2701 gtggcaattt tttcttcaag taatctcagc atccgttctc tcttaagaca aacgtgggta
     2761 aatacccgga cctatcggca gaccgacaag ataccacgcc agcaacatca gcagccatac
     2821 caccaaaaag ataagtggat agggcaagac taacgaatag taagtaccca gtttcgcgtc
     2881 tggtttgtcg cagtctgata gcataaagcg attttttaat ttttctgcct gaagaaatga
     2941 gggcaagcca tcacatggat cgagggtggg ataattccag tcagtgaaat tttaccgtag
     3001 gctatctgtt atttgattct gtggcgtgat agccgccaga caccactgaa ctcgtcactc
     3061 attccccaat cccggcgaga atgtggttat ttacagagct aaaaaatgaa taaaaaaata
     3121 gagcctgtcg acatccgcca gactctacag tacacacagc agtgcatcct cgtcttaaat
     3181 ccggcctggg gtcctggtga ccggtgacag tgttataacg gttttaccca tccgttgcca
     3241 attttcttca gtgacagatt tcacgaaaat ataagttaaa tcatttgtct tgtgattgca
     3301 taattatgca tcgttaatcg acgagtaaat ttaccgggcg agtagcccgt tcgacgctta
     3361 aacatattgc cgaacgattc agctgaggca tagcccagac gatgggcaat tttggtcaac
     3421 gaatatccat catcaagaag cgtcactgca gaaataacgt gcatttgctg aacccagtgg
     3481 cgaaatgtta atcccgtctc tttaacaaat aagcgcatga gtgtacggct actgacgtta
     3541 attttatccg ctagattatc cagtgttggc agataccctt ggttgtttag caaattctca
     3601 caaagctgct gtagccgctc atcatttggc caagggagtt caagtgacat tttcaccccc
     3661 atttttattt cctgaagtat cagtgtctcg agggctgaaa aacgtaactg ttgtttatct
     3721 gtggtacttt gcgacggtgg aagttggtta attgcaatta gtaattccct taacaggggt
     3781 gaaatacccg tcatgaagca aactgttccc agcgtttcac tttccgcttc tgaaaccagt
     3841 acggttttta aaagtacgtt gcctgtcgca acaaacttat gcggcatttg tgggggcagc
     3901 caaagcgcgc tgctggcatg gactagccag acgctttctc cagcccatac acgaacgact
     3961 ccttcagccg ggtaaagcaa ctgggcatag agatgtttat gccagggaac tgtacctcca
     4021 ttcagatgtt ccgaatccag tccgataatg tgatgccctg gtgatgcttt gggttgcagt
     4081 gtaattgtca tttacagtag gtattttgtc agaaatcatc aaataagcca tgttaaattt
     4141 taaatatgac gtatgcaagt ttccggatat atacggtgta aagagtgatg ataatttcta
     4201 aaaaacagct tattggggtg gtagcgatcg gtattctttt gactggcgtt gtctttttta
     4261 tctggagggt aagcaaagga agatttatac agacgacgga tgatgcttac attggtggta
     4321 atatcaccac ggtggcaagt aaagtttctg gttatatttc tgctatagag gtgcgcgata
     4381 atcagagcgt aaaaaaaggc gatattatcc tgcgtctcga tgatcgggat tatcgtgcaa
     4441 atgtcgccag gcttgaggca aaaataaaaa gtagcaaagc caatctcgaa ggcattcaag
     4501 ccactattac gatgcagcag tcgataattc aaagtgccag tgaaacatgg caggcagtaa
     4561 aacatgagga gcaaaagcgc ctgcgagata ctgaacgtta tgaaaaactc gcacaatcag
     4621 cggcaatatc gcaacaaatt attgataacg ccaggtttga ctaccagcag gtcgcagcaa
     4681 aggagcgcaa agccgcgaac gatttcctgg tggagaagca acggctggca gttttatctg
     4741 ctcaggaaga aaacgtacgt gcgagtatcg aagaagttca ggccgctttg actcaggcac
     4801 tactggatct ggaata
//
LOCUS       DAJVPP010000186         4801 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVPP010000186 DAJVPP010000000
VERSION     DAJVPP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.189"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..222
                     /locus_tag="OQM34_003916"
     CDS             <1..222
                     /locus_tag="OQM34_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1454143.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(394..723)
                     /locus_tag="OQM34_003917"
     CDS             complement(394..723)
                     /locus_tag="OQM34_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCS1454144.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(728..913)
                     /locus_tag="OQM34_003918"
     CDS             complement(728..913)
                     /locus_tag="OQM34_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1454145.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(910..3549)
                     /locus_tag="OQM34_003919"
     CDS             complement(910..3549)
                     /locus_tag="OQM34_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1454146.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLSLTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3757..4746
                     /gene="ldhA"
                     /locus_tag="OQM34_003920"
     CDS             3757..4746
                     /gene="ldhA"
                     /locus_tag="OQM34_003920"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1454147.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
BASE COUNT         1195 a         1245 c         1117 g         1244 t
ORIGIN      
        1 agtttgtacc gaatgtttgc cgataaaggt ttggtagtcg cgcaatatat tcgtaaccgt
       61 cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag atgatgaaaa actggcaggc
      121 atcggctttc attggggatt ttctgaccag agtcattttt caacggtatt taagcaacgc
      181 tttgggatga cgccaggcga gtatcgacgt aaattccgct aactaattgg cgttgcatgt
      241 actgcaacgc caattagtta aaacagatat ttgactatgc atttttcagg gcgaaaggca
      301 atttgacagg agtttttgaa tattcacctt gtttcaggaa aatagatcaa tcgctttctc
      361 ctgtagcgtt atcccgatat tgcggttttg ctattaaaac tttcgcaccc atttaccgtt
      421 tatcccttgt acatattccc ccggtttggc ccgagctacg agcttttgcc cggcgagttt
      481 cgcgatatca tctacagaca cattattttg ctttgcccgt tgttggtaac tcgctttacg
      541 ttcggcatta atttcggtta ctaatttctc tgtttcagca tccgttttca gcgcaaccag
      601 ataaccataa aatgtttcac ctacccgccc ctgagttctg gcttcatcca gagtcaatgc
      661 cattacattg ctgcttacca gcccaacaag aaacgcacaa agaagtaatg ttttcttcat
      721 catcgcctca gaaaagatcg ctacgagttt caagcagctc ttcgacatct ttgtctgcct
      781 tgatgatgat ctcatgctca attttaacgt tcatattgat agtgatcggt tccttaggtg
      841 cagcgacttc aatgcgaggc gtacagccaa ccagcataaa agatgacgtc aacgcagcca
      901 gtaaaatttt cattgttttt cctcacactc ttttccttgc ggacagtcat ttcccggcag
      961 acgtgcgttc tgctccaacc atgcctggag attatcgcca aagcgtaaac tgcgccacag
     1021 cgtaaaaata ttctcttcat gatggtaatt aaggtttacc gtaccgcttt taccatcaac
     1081 gcgactggta cctgtaatgt tggcctgcat ggttaataaa ccgagattat ctaagttaat
     1141 ttttgtcgat gaccggctaa tttccatata gcgcaaccag ttaattgctg aacccgcagt
     1201 catattgtct ttcaccaccg catctgcggt atctttatcc agccgcaagg tcattggccc
     1261 gctatttgcc agccaaccat ctttcactat ccatttttcg ttgtttagcc ataacggcaa
     1321 tgcaccacta aatgccccgg acatggcgaa ttgtttcgga ttgacggcgc taaccagttc
     1381 gctggatgag agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg
     1441 tttcatcagc acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaagggttc
     1501 ttcttcagtc cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag
     1561 attaatcact tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc
     1621 atcggcaaaa cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca
     1681 ggcactaccg cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc
     1741 agaaaatgca acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg
     1801 caccagcggc tggaatacag tcagtgattg ttttggccac caggcgttac cgcgcagacg
     1861 aataccgtcc cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc
     1921 tttaaagagg aaataggtcg gattgcgccc atcgacgcta aattttaagg ttgatggcgg
     1981 taacacactg ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa
     2041 ggagggatgc tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt
     2101 ttctaccgtc atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt
     2161 aatcgtgctg tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt
     2221 aaaactccct tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt
     2281 cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat
     2341 ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg
     2401 gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg
     2461 ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata
     2521 taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt gactgttatc
     2581 catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg cttgcccctg
     2641 ggtcagcaca ttcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc
     2701 gactttgaca cccagtcggc cactcaaagg aaaacctgca tacagccagc tccagcgacc
     2761 atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc
     2821 gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcatc
     2881 caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacgggaag
     2941 tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg gctgattttc
     3001 aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa
     3061 tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg agagagagag
     3121 ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt
     3181 aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt gctccgtctg
     3241 cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg
     3301 gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata
     3361 acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat
     3421 acgtgtcccg agcggtagcc agatgtccgc cagcgtggga acccacagcc cgagcgtcat
     3481 cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt
     3541 acccagcatg ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat
     3601 tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga
     3661 atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat atttttagta
     3721 gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca
     3781 aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa
     3841 ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta
     3901 tgtattttcg taaacgatga cggcagccac ccggtgctgg aagagctgaa aaagcacggc
     3961 gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa
     4021 gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac
     4081 gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt
     4141 gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc
     4201 gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg
     4261 cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg tgtggagtat
     4321 gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg cccgctgaca
     4381 ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg
     4441 atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga agcgctgaaa
     4501 aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga tctgttcttt
     4561 gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc tgcctgccac
     4621 aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct
     4681 cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg
     4741 gtttaatctt gcagctcccc tgcaatccag gggagctgat tcagataatc cccaatgacc
     4801 t
//
LOCUS       DAJVPP010000187         4764 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVPP010000187 DAJVPP010000000
VERSION     DAJVPP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.190"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(684..1100)
                     /locus_tag="OQM34_003921"
     CDS             complement(684..1100)
                     /locus_tag="OQM34_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454148.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1127..1267
                     /locus_tag="OQM34_003922"
                     /pseudo
     CDS             1127..1267
                     /locus_tag="OQM34_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1267..2292
                     /locus_tag="OQM34_003923"
     CDS             1267..2292
                     /locus_tag="OQM34_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1454149.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2345..2434)
                     /locus_tag="OQM34_003924"
     tRNA            complement(2345..2434)
                     /locus_tag="OQM34_003924"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2398..2400),aa:Ser,seq:cga)
     gene            2528..3325
                     /gene="mtfA"
                     /locus_tag="OQM34_003925"
     CDS             2528..3325
                     /gene="mtfA"
                     /locus_tag="OQM34_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1454150.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3426..3501
                     /locus_tag="OQM34_003926"
     tRNA            3426..3501
                     /locus_tag="OQM34_003926"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3459..3461,aa:Asn,seq:gtt)
     gene            3663..4764
                     /locus_tag="OQM34_003927"
                     /pseudo
     CDS             3663..4764
                     /locus_tag="OQM34_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         1307 a         1123 c         1071 g         1263 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
       61 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      121 tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
      241 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
      301 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
      361 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
      421 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
      481 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
      541 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
      601 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
      661 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
      721 ttaatctggt tgccggatgc tggtggattt tcgcgtttta attgttcata aaagtgcaca
      781 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
      841 tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc
      901 cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc
      961 ggctctgctt ccagcgatgc ccgtgcaatt ttgaataact cgccctctac tcgcgccatc
     1021 cctgaattgg ggtggcattt cgtaatcgct atttttaatt tggcttcttc gattaattgt
     1081 tcttttgtta attcagtcat tttcattacc gccctggtaa tgctgccacc agcgaaaatg
     1141 gttaatggaa aacgggcttc cgttctatac caatcgtagt ggcaaaccga ttgtcagccg
     1201 ggatctattt acctgcaata aaactttacc accacgcgag gtagagccga attttggtgc
     1261 gatctgatgg gaagacgaag gaaaaatcct gaacacgaaa aattacctcc aaatgtatac
     1321 ccaaataaat atagttatgt atggaaacca acatccagag aatctgtaac actaaccgcc
     1381 atcaaggatg gtttagctgc tttatggaaa aagtatgagg aaactgtaaa taatcgcgat
     1441 cgtgcaatga cattcggtcg cttgtgggaa aaattcctcg ccagcgccta ttacagtgac
     1501 ctcagtccaa gaacacaaaa agattatctg caacatcaaa aaaagttgct tgccgtattc
     1561 ggtaaggtgc cggcagattc cataaaacca gaacacatcc gtcgatacat ggacaagaga
     1621 ggggaacaga gtaaaacgca agccaaccat gaaaaaagca gtatgtcccg cgtttacagt
     1681 tgggggtatg agcgagggta cgtgaaggct aacccatgtg caggtgtaag taaattcaag
     1741 gccaaaaacc gcgaacgata tgtaaccgac aaagaatacc aggcagtatt aagcgttgca
     1801 cctcttcctg tttttatcgc aatggaaatt gcctatctgt gtgcagcgag ggtttccgat
     1861 gtgttatcgc tgaaatggga gcagattgga aacgacggga tctttatcca gcaagggaaa
     1921 acaggaaaaa aacagataaa agcatggagt ccacgattac aggcggcgat cgaaaaagca
     1981 aaacagttac caacatccgc ctatgtaatc agcaatcaat acggcaaccg atatatgtac
     2041 aaaggcttta acgaaatgtg ggtagaagca agaaatcgcg caggcaaaat ttcaggtatt
     2101 ttaaccgact tcacctttca tgatctgaag gcgaaaggaa tttcagacta tgaaggaagc
     2161 agtcgggata agcaactttt ctctggtcac aaaaccgagg ggcaagtgct aatctatgac
     2221 aggaaggtta aagtttcacc gacacttgat gtcccgttac ctgaaaatat tccaagaaaa
     2281 tattccaagt aattccaagt gtgatttttg tcactgactt aatgatgtat aagtgattga
     2341 attttggcgg agagaggggg atttgaaccc ccggtggagt tgcccccact ccggttttcg
     2401 agaccggtcc gttcagccgc tccggcatct ctccgttcag atggttgcca tgatgccagg
     2461 aaatttggca ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag
     2521 caaaacgatg attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc
     2581 ctggcaggaa gcactatcga tccccctttt aacgtgtctg acagaacagg aacaaagcaa
     2641 attggtcgct cttgccgaac gttttttaca gcaaaaacgg cttgttcctt tacagggctt
     2701 tgagctgaat tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga
     2761 gttaggactg gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt
     2821 ggtcgatgat gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc
     2881 aggtcagagc tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt
     2941 tgatgcttct ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa
     3001 cggcgatcgc gccagcggag ttccctttat ttcgttgcgt gaggttgctg gctgggaaca
     3061 cgatcttcat gctgcaatga acaacattca ggaagaaatc gaattagttg gtgagaatgc
     3121 ggcgagcatt gatgcttatg ctgccagtga tcctgctgaa tgttttgccg tactttctga
     3181 atatttcttt agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt
     3241 ctgtcaattt tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc
     3301 gttttcagcg acgaatgttc attaattaac aactttgaag attaattaac caattgaaat
     3361 ggcttatgaa atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc
     3421 acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     3481 cgagtccagt cagaggagcc aattttctgt tttcatgcat ccttgcgaat ctttatctga
     3541 tgttgattca acaggttagt gtgaaaactc ttctcggtgc attttgattt taccctacgc
     3601 atcgggaaaa attggtggtc aaatctggga tcaggttagt tcgataatgg agtgaccccc
     3661 atatatccct taccgacgca aaaatccgcc ccctcaagcc ttctgataaa ccctttaaag
     3721 tctccgattc tcacggtctg tatctgctgg tcaagccggg tggctcccgc cactggtatc
     3781 tcaaataccg tattagcggt aaagaatccc gcattgcgct gggtgcctat ccagccatct
     3841 ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg ctgaatatca
     3901 acccggtaca gcagcgggct gctgaacgtg gctcacgaac accggagaaa gtttttaaaa
     3961 acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc
     4021 tgcttgccag cctgaacaat cacatctttc cggtcatcgg gaacctacct gtatcagaac
     4081 ttaaaccccg tcatttcatt gacctgctga aagggatcga ggaaaaaggt ctgctggagg
     4141 ttgcgtcccg cacacggcag cacctgagta acataatgcg ccatgcggtc catcaggagt
     4201 taatcgatac gaaccctgca gcaaaccttg gcggcgtgac cacacctcct gtcagacggc
     4261 actatcctgc cctgccgctg gagcggctgc ctgaactgct tgaacgtatt ggggcatatc
     4321 atcagggccg tgaactgacc cggcatgccg ttctgctgat gctgcatgtg ttcattcgct
     4381 ccagtgaact gcgtttcgcc cgctggtcag agattgattt cacaaaccga gtctggacga
     4441 tacccgcgac gcgagaaccc attattggcg tgcgttattc cggccgcggg gcaaaaatgc
     4501 gaatgccgca tatcgtcccc ctctcagaac agtccatcgc cattctgaaa cagattaagg
     4561 atatcaccgg taataatgaa ctgatcttcc ccggcgacca taacccgtat aagccaatgt
     4621 gtgaaaacac ggtcaataag gcactgcggg tgatgggtta cgacacgaaa aaggatatct
     4681 gcggtcacgg cttccgggca atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa
     4741 aggacgcagt agaacgccag atga
//
LOCUS       DAJVPP010000188         4721 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVPP010000188 DAJVPP010000000
VERSION     DAJVPP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.191"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..583)
                     /gene="kptA"
                     /locus_tag="OQM34_003928"
     CDS             complement(<1..583)
                     /gene="kptA"
                     /locus_tag="OQM34_003928"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCS1454151.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            833..1516
                     /locus_tag="OQM34_003929"
     CDS             833..1516
                     /locus_tag="OQM34_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCS1454152.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1513..1974
                     /locus_tag="OQM34_003930"
     CDS             1513..1974
                     /locus_tag="OQM34_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCS1454153.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            1987..3159
                     /gene="iadA"
                     /locus_tag="OQM34_003931"
     CDS             1987..3159
                     /gene="iadA"
                     /locus_tag="OQM34_003931"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1454154.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3224..4135
                     /gene="hypT"
                     /locus_tag="OQM34_003932"
                     /gene_synonym="qseD"
     CDS             3224..4135
                     /gene="hypT"
                     /locus_tag="OQM34_003932"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1454155.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYARRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4128..4520)
                     /gene="iraD"
                     /locus_tag="OQM34_003933"
     CDS             complement(4128..4520)
                     /gene="iraD"
                     /locus_tag="OQM34_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCS1454156.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4517..4600)
                     /gene="idlP"
                     /locus_tag="OQM34_003934"
     CDS             complement(4517..4600)
                     /gene="idlP"
                     /locus_tag="OQM34_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCS1454157.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1094 a         1245 c         1199 g         1183 t
ORIGIN      
        1 attgtttttg taccccagtc ggcataaacg accactcaag aaactcaaca gcaaccgttg
       61 atgtcagcca gaccccgttt tccgcttgcc agaaagggat gcctcgtttc gccatttcct
      121 gtgctttgac ggttaagaca accggcgatc cgtgtcttgc tcccactttg cgtgccgttg
      181 cttcatcagc agagagatgc acgtaatgac gatcccctgc aatcagcccc tgttttttta
      241 tctcgtcaag gaaacggctt gccgtaccgt ggtagagaaa ctgtggcggt gttttttcgg
      301 caaaggaaat agcgacctgc gaagtcgaat gcccctgaac cgcgcggatg cacctaccat
      361 cactggaata actaaaacgc tttttatcgc tggttgcgac cacggtatcc agcaatgcac
      421 gggttaatcg cttgcccgct ttttgggcac aaagtatgag cttattgata tccgcccatc
      481 cttcacggtc cagtacgata ccaatcgcct ccggtttgtg tcggaggaca aaacttaaaa
      541 acttgctcgt atccgccagc tctttttcgt tgtactttgc catcgcgttt tcgtccctgg
      601 attttctgtt tttatttact aatgtcattg tcactgggtt tatgaatttt ctgcaactca
      661 atcctgcgca gaaccttttc cacacagcgt aatttttccc ctatgcaaaa aacgcatagc
      721 cagaaacata cttgcatttt ccccataccc acatggctga taaggttttt cagttccagg
      781 aattttcctg gctcaagcaa cacacaaaca caaaaaaaca atggggattg ttatgacaca
      841 acaaggggat gctgtagccg gggagctggc gacagaaaaa gtgggcatta aagggtatct
      901 ggcttttttt ctcaccataa tcttttttcc cggtgttttt tccggaacag acagctggtg
      961 gcgcgttttt gatttttccg ttctgaacgg atcgtttggg caactgcctg gtgcgaatgg
     1021 agccaccacc tcatttcgcg gggtgggtgg cgcgggggct aaagatggct ttctctttgc
     1081 gctggagctg gcaccgtcag tcattctctc tctggggatt atttcaatta ccgatggtct
     1141 tggtggatta cgcgccgcgc agcagttaat gacacctgtg ctaaaaccgt tgttgggcat
     1201 tccagggatc tgctcgctgg cactgatcgc taacctgcaa aacacggatg ccgccgccgg
     1261 tatgaccaaa gagctggcgc aggaaggtga aattaccgaa cgcgataaag tgattttcgc
     1321 cgcatatcaa accagcggta gcgccatcat caccaactac ttctcttccg gcgtcgcggt
     1381 gtttgccttt ctgggcacgt ccgtaatcgt acctttagcc gtcatcctgg tgtttaaatt
     1441 tgtcggagcc aacattttgc gcgtctggct taacttcgaa gaacgtcgca acccaacgca
     1501 aggagcgcaa gcatgacaac tcaggtccgc aaaaacgtca tggatatgtt tatcgatggc
     1561 gcgcgccgtg gctttactat cgccacgacc aacctgctgc caaacgtggt gatggcgttt
     1621 gtcatcattc aggcgctgaa aattaccggc ctgctcgact gggtggggca tatctgtgaa
     1681 cccgtaatgg cgctgtgggg attacccggt gaagcggcaa ccgtcctttt agccgcatta
     1741 atgagtatgg gcggcgcagt cggtgttgcc gccagtctgg caaccgctga cgcattaacc
     1801 ggccacgatg tcaccgtttt actgcccgca atgtatttaa tgggcaaccc ggtgcaaaat
     1861 gtcggtcgct gcctggggac agccgaagtg aatgccaaat attacccgca tatcattacg
     1921 gtctgcgtga ttaacgcatt gctgtcgatc tgggttatgc agcttattgt ttaacaagga
     1981 gttaccatga ttgattatac cgcagccggt tttaccctgc tgcagggagc gcatttgtat
     2041 gcgccggaag atcggggaat ttgcgatgtc ctcgtcgcta acggcaaaat tatcgccgtt
     2101 gccagcaata tcccttctga cattgtaccg aactgcacgg ttgtcgatct cagcgggcag
     2161 atcctctgcc caggttttat tgatcaacac gtccatttga ttggcggtgg cggcgaagca
     2221 ggtcccacga cgcgcacgcc ggaagtggcg ctaagtcgcc tgacggaagc gggcgtcacg
     2281 tcagtggttg gtctgctggg caccgactct atctctcgcc acccggaatc cctgctcgcc
     2341 aagacccgtg cgctcaatga agaaggcatc agcgcctgga tgctgaccgg cgcttatcat
     2401 gtcccttccc gcactattac gggttccgtg gaaaaagacg tggcgattat cgatcgtgtg
     2461 attggcgtga aatgcgccat ctctgatcac cgttctgccg caccggacgt ttatcacctg
     2521 gctaatatgg cggcagaatc ccgcgttggc ggtttgctcg gcggtaaacc tggcgtcacc
     2581 gtgttccaca tgggcgacag taaaaaggcg ttaaagccta tttatgacct gctggaaaac
     2641 tgcgatgtgc cgatcagcaa gctgctgccg acccacgtta accgcaacgt accgttgttt
     2701 gagcaggcgc tggagttcgc gcgcaaaggc ggcaccatcg atatcaccag cagcattgac
     2761 gaaccggtcg cccctgccga aggtattgcc cgcgccgttc aggcgggtat tccgctggca
     2821 cgcgtcaccc tcagctccga cggcaacggt agccagccgt tcttcgatga cgaagggaat
     2881 ttaacccata tcggtgttgc aggttttgaa acgttgctgg aaaccgtgca ggtgctggtc
     2941 aaagactatg atttcagtat cagcgatgcc ctgcgcccgc tcaccagtag cgtagccggt
     3001 ttccttaaca tgagcgggaa aggcgaaatt ctgccaggca atgacgcaga cttactggtc
     3061 atgacgccag aattgcgcat tgagcaggta tacgctcgcg gcaaactgat ggtcaaagac
     3121 ggcaaagcct gcgtgaaagg aacgtttgaa acggcttaaa cggttgtcag ccgccctaat
     3181 ggacgtagaa tgcccccaag ggcggctgac agagtaaaac gtaatggatg actgtggtgc
     3241 ggttttgcat aatattgaaa ccaaatggct ttatgatttt ctgactctgg aaaaatgccg
     3301 caatttttcc caggcggcag tcagtcgcaa cgtctcgcaa ccggcattca gccgccgcat
     3361 ccgtgcactg gaacaggcga ttggcgttga attgtttaac cgccaggtga cgccactgca
     3421 actctcagaa caagggaaaa tcttccattc gcagatccgc catctgttgc aacagttaga
     3481 aagcaacctt gcagagctgc gtggcggcgg cgattacgcg cgacgtaaaa tcaagatagc
     3541 cgctgcacac tctctttccc tcggtctgtt accgtccatt atcagccaga tgccgccgct
     3601 ctttacctgg gcaattgaag ccattgatgt cgatgaagcg gtcgataaac tgcgtgaagg
     3661 gcaaagtgac tgtatttttt cctttcacga cgaagatttg ctggaagcgc cgtttgacca
     3721 cattcgctta tttgaatctc aattgttccc tgtctgcgcc agtgacgaac acggagaagc
     3781 actttttaac ctcgcgcagc cacactttcc gttactgaat tacagccgca actcctacat
     3841 ggtgcgattg attaatcgca ccctgacgcg ccacagtgag ttaagtttca gcaccttttt
     3901 tgtctcttcg atgagcgagc ttttaaagca ggttgccctc gacggctgtg ggattgcctg
     3961 gctgccggag tatgccatcc aacaagaaat tcgcagcggg cagctcgttg tgctgaatcg
     4021 ggacgaacag gtgatcccga ttcaggccta cgcataccgg atgaataccc gcatgaatcc
     4081 agttgccgaa cgcttctggc gtgaactgcg cgagctggag attgtgctta gctgacattc
     4141 tcaagcgtcg cactgcgcca accgccatta tcccagacca gatcaaacgt tagcggttca
     4201 tcgtcgtggt acagcattat ctcaaggctg atcgccagag tgtggcagta cgcttctttc
     4261 aaattaacct gcaacgccgc aatccgtggc tcatgaaata acagtgcaga acgcagctga
     4321 cggcaatacc agctctggag atgtttgtcg ctcgggttat gcacttgcca ctcgggcatg
     4381 cctgaaacaa gtaatgacgg taatgccgaa tgaggagaat taaaaagagt gaggagatcg
     4441 ctgcataccg atttacgcag caatgacgtt ttattgcctg aaatttcagg taaaacagcc
     4501 tgaagtgatt ggcgcatcat tttgcgcact cctgacgttt agcaacatat gcggcttgcc
     4561 ctgaacaccg ggcaccactg tattgatgct cattttccat agccattccc ttttgttcat
     4621 tacatccatg tcagtctgag acaggcctga atcctgtcag caaagtataa aaccttactt
     4681 gcctatgaat atctactgac ttttcaggaa aaaagtcatg a
//
LOCUS       DAJVPP010000189         4691 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVPP010000189 DAJVPP010000000
VERSION     DAJVPP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.192"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1796
                     /gene="mutS"
                     /locus_tag="OQM34_003935"
     CDS             <1..1796
                     /gene="mutS"
                     /locus_tag="OQM34_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1454158.1"
                     /translation="SITMEREQDSIIMDAATRRNLEITQNLAGGAENTLASVLDCTVT
                     PMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDFTAELQPVLRQVGDLERILARLALR
                     TARPRDLARMRHAFQQLPELRAQLETVDSAPVQALREKMGEFAELRDLLERAIIDTPP
                     VLVRDGGVIASGYNEELDEWRALADGATDYLERLEVRERERTGLDTLKVGFNAVHGYY
                     IQISRGQSHLAPINYMRRQTLKNAERYIIPELKEYEDKVLTSKGKALALEKQLYEELF
                     DLLLPHLEALQQSASALAELDVLVNLAERAYTLNYTCPTFIDKPGIRITEGRHPVVEQ
                     VLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYMRQTALIALMAYIGSYVPAQKVEIG
                     PIDRIFTRVGAADDLASGRSTFMVEMTETANILHNATEYSLVLMDEIGRGTSTYDGLS
                     LAWACAENLANKIKALTLFATHYFELTQLPEKMEGVANVHLDALEHGDTIAFMHSVQD
                     GAASKSYGLAVAALAGVPKEVIKRARQKLRELESISPNAAATQVDGTQMSLLSVPEET
                     SPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            1902..2554
                     /gene="pphB"
                     /locus_tag="OQM34_003936"
                     /pseudo
     CDS             1902..2554
                     /gene="pphB"
                     /locus_tag="OQM34_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2605..3372)
                     /locus_tag="OQM34_003937"
     CDS             complement(2605..3372)
                     /locus_tag="OQM34_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1454159.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            3568..4476
                     /locus_tag="OQM34_003938"
     CDS             3568..4476
                     /locus_tag="OQM34_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1454160.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            4473..>4691
                     /locus_tag="OQM34_003939"
     CDS             4473..>4691
                     /locus_tag="OQM34_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCS1454161.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1162 a         1193 c         1215 g         1121 t
ORIGIN      
        1 gttctatcac catggaacgt gagcaggaca gcatcattat ggatgccgcg acacgtcgta
       61 acctggaaat tactcagaac ctggcgggcg gtgcggaaaa tacgctggct tctgtgctcg
      121 actgcaccgt cacgccgatg ggtagtcgta tgctgaaacg ctggctgcat atgccagtgc
      181 gcgatacccg cgtgttgctt gagcgccagc aaactattgg cgcattgcag gatttcaccg
      241 ccgagttgca gccggtactg cgtcaggtcg gcgacctgga acgtattctg gcacgtctgg
      301 ctttacgaac tgctcgccca cgcgatctgg cccgtatgcg ccacgctttc cagcaactgc
      361 cggagctgcg tgcgcagtta gaaactgtcg atagtgcacc ggtacaggcg ctacgtgaga
      421 agatgggcga gtttgccgag ctgcgcgatc tgctggagcg agcaatcatc gacacaccgc
      481 cggtgctggt acgcgacggt ggtgttatcg catcgggcta taacgaagag ctggatgagt
      541 ggcgcgcgct ggctgacggc gcgaccgatt atctggagcg tctggaagtc cgcgagcgtg
      601 aacgtaccgg cctggacacg ctaaaagttg gctttaatgc ggtgcacggc tactacattc
      661 aaatcagccg tgggcaaagc catctggcac ccatcaacta catgcgtcgc cagacgctga
      721 aaaacgccga gcgctacatc attccagagc taaaagagta cgaagataaa gttctcacct
      781 caaaaggcaa agcactggca ctggaaaaac agctttatga agagctgttc gacctgctgt
      841 tgccgcatct ggaagcgttg caacagagcg cgagcgcgct ggcggaactc gacgtgctgg
      901 ttaacctggc ggaacgggcc tataccctga actacacctg cccgaccttc attgataaac
      961 cgggcattcg cattaccgaa ggtcgccatc cggtagttga acaagtactg aatgagccat
     1021 ttatcgctaa cccgctgaat ctgtcgccgc agcgccgtat gttgattatt accggcccga
     1081 acatgggcgg taaaagtacc tatatgcgcc agaccgcact gattgcgctg atggcctaca
     1141 tcggcagcta tgtaccggca caaaaagtcg agattggacc tatcgatcgc atctttaccc
     1201 gcgtaggcgc ggcagatgac ctggcttccg ggcgctcaac ctttatggtg gagatgactg
     1261 aaaccgccaa tattttacat aacgccaccg aatacagtct ggtgttaatg gatgagatcg
     1321 ggcgtggaac gtccacctac gatggtctgt cgctggcgtg ggcgtgcgca gaaaatctgg
     1381 cgaataagat taaggcattg acgctgtttg ccacccacta tttcgagctg acccagctac
     1441 cggagaaaat ggaaggcgtc gccaacgtac atctcgatgc gctggagcac ggcgacacta
     1501 tcgcctttat gcacagcgtg caggacggcg cggcgagcaa aagctacggt ctggcggttg
     1561 ctgctctggc gggcgtaccg aaagaggtta ttaagcgcgc acggcaaaaa ctgcgtgagc
     1621 tggaaagcat ttcgccgaac gccgccgcga cgcaagtgga tggtacgcag atgtctttgc
     1681 tgtcagtgcc agaagaaact tcgcctgcgg tcgaagcact ggaaaatctc gacccggatt
     1741 cactgactcc gcgtcaggcg ctggagtgga tttatcgctt gaagagtctg gtgtaataac
     1801 aattcccgat agtcttttgc tatcgggaat attaacgaca actgacgaat caaataaaaa
     1861 taccctgtat aataggaaag cttattttac agggtaaaac catgccatct acacgctacc
     1921 aaaaaatcaa tgcccatcac tatcgccata tatgggtcgt tggtgatatt catggtgaat
     1981 atcagttatt acaatcccgc ttacatcaac tctctttttt ccccgaaacc gacttactta
     2041 tttctgtcgg cgataatatt gatcgtgggc cggagagtct tgacgtcctg cgcctgctaa
     2101 accagccctg gtttatctcc gttaaaggca accacgaagc aatggcgctg gatgcgttcg
     2161 cgaccggcga tggcaatatg tggcttgcca gcggtggtga ctggtttttc gatttaaatg
     2221 attgcaacaa gaggcaatag atctgttgct gaaattccat caccttccac atattattga
     2281 aatcactaac gacataataa aatatgtcat cgcacatgca gattatccgg gtgatgaata
     2341 tctctttggc aaagaaatag cggagagcga attactctgg cctgtttatc gtgtgcagaa
     2401 atcgcttaat ggcgagttac aacaaataaa cggcgctgat tattttatat ttggacatat
     2461 gatgtttgat aacattcaga cgttcgctaa ccagatttat attgataccg gattgccgaa
     2521 aagtgggcgg ctgtcatttt ataaaataag gtagtctcat gcttcttctg tgaagcatga
     2581 gtaaccagat gttattgcag gccattattc atttttcgct accagcaaag agagatcctg
     2641 cttcgccagc gcgcgactgg cactctccgg caaaccgtcg tctgtaataa tctgatcaaa
     2701 ctcgcttaat ggtaacgcca gccatgtcgc cacctgacca tatttcgtcg catcacagac
     2761 caaaactcgc tggcggctgg cactggcaat cgcccgtttc accgtgactt tatcttccgc
     2821 tggcgtagaa atcccccgca cactccatga cgatgcagaa ataaaagcct gatcaatcat
     2881 caggctgcac agcatggtcg cagcggcttc cccgacacag gaacggtttt cccgacacac
     2941 tgcaccgcca gtgtgaataa ttgtgcaatt actgttgtcg agcaagtagt tcgcaataac
     3001 gaaatcgttt gtgaccacag tcagtgactc catgtgaatc agatgctgtg ctatcgctaa
     3061 cgtggtcgtt cccgcatcca gatagataca acttcccggc agaacaagac ttgccgccag
     3121 cttgccaata gccgcttttt gcgtcattgc cagcgcagtt tttacctgat gagaaggttc
     3181 atgcgccacg cgtcccggag actggacgcc tccggacacc agcacaacgg ctccctgctg
     3241 ctccagtttt tgtaaatccc gacgaatggt catatgtgac acattaattc tgtccgttag
     3301 ttcagcaata ctgacaatgc ctttttcagc taccatctca aggatgattt ggcgacgctc
     3361 tacgggtatc aacttttgct ccttcctttg tcctgctgac attctacgct atttgcctgc
     3421 gaaacgtgcg cggcgcaact aacgcttagt tcacataaaa taacacaaaa tgttaattta
     3481 tgtgaatcat ctcaccatat cgttatctcc cagcgcttat gttcacaata tcaaacaaaa
     3541 tatcacttaa attaacaagg agagcagatg aaaacgggat ctgagtttca tgtcggtatc
     3601 gttggcttag ggtcaatggg aatgggagca gcactgtcat gtgtccgcgc aggtttttct
     3661 acctggggcg cagacctgaa cagcaatgcc tgcgctacgt tgaaagaggc aggtgcttgc
     3721 ggggtttctg ataacgccgc gacgtttgcc gaaaaactgg acgctctgct ggtgctggtg
     3781 gtcaatgcgg cccaggttaa acaggtgctg tttggtgaaa caggcgttgc acaacatctg
     3841 aaacccggta cggcagtaat ggtttcttcc actatcgcta gtgctgatgc gcaagaaatt
     3901 gctaccgctc tggctggatt cgatctggaa atgctggatg cgccagtttc tggtggtgca
     3961 gtaaaagccg ctaacggtga aatgactgtc atggcctccg gtagcgatat tgcctttgaa
     4021 cgactggcac ccgtgctgga agccgttgcc ggaaaagttt atcgcatagg tgcagaaccg
     4081 ggactaggtt cgaccgtaaa aattattcac cagttgttag cgggcgtaca tattgctgcc
     4141 ggagccgaag cgatggcact tgcagcccgt gcggggatcc cgctggatgt gatgtatgac
     4201 gtcgtgacca atgccgccgg aaattcctgg atgttcgaaa accggatgcg tcatgtggtg
     4261 gatggcgatt acaccccgca ttcagccgtc gatatttttg ttaaggatct tggtctggtt
     4321 gccgatacag ccaaagccct gcacttcccg ctgccattgg cctcaacagc attgaatatg
     4381 ttcaccagcg ccagtaacgc gggttacggg aaagaagacg atagcgcagt tatcaagatt
     4441 ttctctggca tcactctacc gggagcgaaa tcatgatcaa gattggcgtt atcgccgatg
     4501 attttaccgg cgcgacggat atcgccagtt ttctggtgga aaacggtcta ccaacggtac
     4561 aaattaacgg tgttccaaca ggtaaaatgc cggaagcaat cgacgcactg gtgatcagcc
     4621 tgaaaacgcg ctcctgtccg gtggttgaag ccacacagca atcgctggcg gctctgagct
     4681 ggttgcaaca g
//
LOCUS       DAJVPP010000190         4499 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVPP010000190 DAJVPP010000000
VERSION     DAJVPP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.193"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(146..751)
                     /gene="azoR"
                     /locus_tag="OQM34_003940"
     CDS             complement(146..751)
                     /gene="azoR"
                     /locus_tag="OQM34_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1454162.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            952..>4499
                     /gene="hrpA"
                     /locus_tag="OQM34_003941"
     CDS             952..>4499
                     /gene="hrpA"
                     /locus_tag="OQM34_003941"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1454163.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLS"
BASE COUNT         1117 a         1142 c         1234 g         1006 t
ORIGIN      
        1 atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag gaagagcaca
       61 aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac tgaatatcta
      121 cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg gctgcttttg
      181 cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg gcgaagacaa
      241 atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc gtcaccaggt
      301 ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct tttttacccg
      361 ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg cgtgcaacca
      421 ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt gccgcaataa
      481 cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc agagcttcct
      541 gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt tcgccatcca
      601 gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg gagtgctttt
      661 cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac cctgccagga
      721 tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt ttgaatggac
      781 gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagata gcgcaataaa
      841 tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt ggtactctat
      901 atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt tatgacagaa
      961 caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat gctgcgcgac
     1021 agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc tgatgcacaa
     1081 caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa agtcctgctg
     1141 cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag tcagaaaaaa
     1201 caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg ggaaaccggc
     1261 tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg tattaaaggg
     1321 ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa ccgtattgcg
     1381 gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt cagcgatcac
     1441 gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc ggagatccag
     1501 caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca cgaacgcagc
     1561 ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg tcctgaccta
     1621 aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca ctttaataat
     1681 gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta tcgcccgatt
     1741 gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga cgccgtagac
     1801 gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga gcgggaaatt
     1861 cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat cttgccgctt
     1921 tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag cggacggcgc
     1981 attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat taaatacgtc
     2041 atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt gcagcgtttg
     2101 ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg tggtcgtgtg
     2161 tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg cccggagttt
     2221 accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat gaccgcgctg
     2281 gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg caatatccag
     2341 gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca ggccagcgcc
     2401 tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc acgtctggcg
     2461 cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat tatcacgtcc
     2521 gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc atcggacgaa
     2581 aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa tctgtggaat
     2641 tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct gtgtcgtacc
     2701 gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca gttgcgccag
     2761 gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg tgaaattcac
     2821 atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga taaacaggaa
     2881 tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt attcaaaaag
     2941 ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg ggggcgcatt
     3001 gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat taaacgcacc
     3061 tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga aaaagtcact
     3121 gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat cgattcggcg
     3181 ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca gacgcgtcac
     3241 gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga acacaaatca
     3301 cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga ccagcgcatc
     3361 agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt cagccgcgaa
     3421 acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc ggaaaaaatc
     3481 agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg tttgagctat
     3541 cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct gccgttactt
     3601 aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg cgaattggtg
     3661 attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc tgcaccaaac
     3721 tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct cgacagcctt
     3781 gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg gcactgggat
     3841 caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa caagaagcta
     3901 aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt gcaggaaacg
     3961 ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg gagttttggt
     4021 cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc gtggccggcg
     4081 ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct ggagcaaaaa
     4141 caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc gccgatcaag
     4201 tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa cccgtatggc
     4261 aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact gatcgacgcc
     4321 aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa agtgcgcgcc
     4381 gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct cactgcggtg
     4441 ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact ggggctttc
//
LOCUS       DAJVPP010000191         4338 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVPP010000191 DAJVPP010000000
VERSION     DAJVPP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.194"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            37..111
                     /gene="tnaC"
                     /locus_tag="OQM34_003942"
     CDS             37..111
                     /gene="tnaC"
                     /locus_tag="OQM34_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCS1454164.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            332..1747
                     /gene="tnaA"
                     /locus_tag="OQM34_003943"
     CDS             332..1747
                     /gene="tnaA"
                     /locus_tag="OQM34_003943"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCS1454165.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1838..3085
                     /gene="tnaB"
                     /locus_tag="OQM34_003944"
                     /pseudo
     CDS             1838..3085
                     /gene="tnaB"
                     /locus_tag="OQM34_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
     gene            3217..>4338
                     /gene="mdtL"
                     /locus_tag="OQM34_003945"
     CDS             3217..>4338
                     /gene="mdtL"
                     /locus_tag="OQM34_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1454166.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMF"
BASE COUNT          985 a         1030 c         1069 g         1254 t
ORIGIN      
        1 taatgtagcg gcgtgtcttt cgaggataag tgcattatga atatcttaca tgtatgtgtg
       61 acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta atttaccctt
      121 ctgtagccat caccagagcc aaaccgatta gattcaatgt gatcgattag tttgctatat
      181 cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta gtaaatgatg
      241 gtgcttgtat atatacatgg tgaattaatg gatattgcag atgtaatatt cacagggatc
      301 actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc tccctgaacc
      361 gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc gtgaagaagc
      421 aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt ttatcgattt
      481 actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga tgatgcgcgg
      541 cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag tgaaaaatat
      601 ctttggttat caatatacta ttccgactca ccagggccgt ggcgcagagc aaatctatat
      661 tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca aaatggtggc
      721 attctctaac tatttctttg ataccacgca gggccatagc cagattaacg gctgtaccgt
      781 gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg cgttacgact ttaaaggcaa
      841 ctttgacctc gaaggattag aacgcggtat tgaagaagtt ggcccgaata acgtgccgta
      901 tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac tggcaaactt
      961 aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg actccgcacg
     1021 ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag actggaccat
     1081 cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt ccgccaagaa
     1141 agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct tctttgatgt
     1201 gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat atggcggcct
     1261 ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga atctcgactg
     1321 gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag agattggcgt
     1381 tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac tgctgccgca
     1441 tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata aagtcgccgg
     1501 tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa ccggtaaaca
     1561 actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat atactcaaac
     1621 acatatggac ttcattattg aagcctttaa acatgtaaaa gagaacgcgg cgaatattaa
     1681 aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa aactgaaaga
     1741 agtttaatta atactacaga gtggctataa ggatgttagc cactctctta ccctacatcc
     1801 tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag ctgaaaaaaa
     1861 gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg gaggtatgtt
     1921 tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta tccttatcat
     1981 tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt taaattatcc
     2041 tgtcggctcc agttttaaca ccatcaccaa agatttaata ggtaacacct agaacattat
     2101 cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata tctctgctaa
     2161 tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta atccacgtat
     2221 tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt cgttagccgc
     2281 cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga tcgtgtttgg
     2341 ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca ccactgcggg
     2401 aacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt catttggttt
     2461 ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata agttaatcaa
     2521 aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc tctattgcac
     2581 gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg gcaacattga
     2641 ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt tttgcctgct
     2701 ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg ggttgttcga
     2761 ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca aaaccgtgct
     2821 gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct ttatttacgg
     2881 gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg cagtgcttgc
     2941 aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg gcaatcttat
     3001 tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg gcaacgtctt
     3061 taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc tggcttcttg
     3121 cctctcagga aatcacttat gtccaaatgg caactcgcct tatcctcctt caccacgtat
     3181 gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt gatttgtagt
     3241 tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg tttaccgcgc
     3301 atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc cgtatatctg
     3361 gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg ccgatcgttc agggagaaag
     3421 ccggtcgcca tacccggcgc ggcgctattt attattgcct cggtgttctg ttcactggct
     3481 gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc aggctgttgt
     3541 tacgtggtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg ggctaaagtg
     3601 ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt gctcggacat
     3661 ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat gatgggcatc
     3721 gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc ccccgctgct
     3781 tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc gttttttcct cagccgtgtt
     3841 gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc gccggtattg
     3901 ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca ccattatggc gctgaccgct
     3961 ggcgtcagca tgaccttttc attctcgacg ccatttgcgc tgggaatttt taagccacgt
     4021 acgttgatga tcacctcgca ggtgttattc cttgcggcgg ggatcaccct tgccgtttca
     4081 ccttcccatg cggtttctct gtttggtatc acgctgattt gcgccggttt ctcggtaggt
     4141 tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg cgtagccagc
     4201 tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct ggccgcggtg
     4261 gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag catagtgagc
     4321 ctgttgctga ttatgttc
//
LOCUS       DAJVPP010000192         4328 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVPP010000192 DAJVPP010000000
VERSION     DAJVPP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.195"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..622)
                     /gene="ycjM"
                     /locus_tag="OQM34_003946"
     CDS             complement(<1..622)
                     /gene="ycjM"
                     /locus_tag="OQM34_003946"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCS1454167.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEV"
     gene            complement(834..1181)
                     /gene="pspE"
                     /locus_tag="OQM34_003947"
     CDS             complement(834..1181)
                     /gene="pspE"
                     /locus_tag="OQM34_003947"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1454168.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1224..1445)
                     /gene="pspD"
                     /locus_tag="OQM34_003948"
     CDS             complement(1224..1445)
                     /gene="pspD"
                     /locus_tag="OQM34_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1454169.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1454..1813)
                     /gene="pspC"
                     /locus_tag="OQM34_003949"
     CDS             complement(1454..1813)
                     /gene="pspC"
                     /locus_tag="OQM34_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1454170.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1813..2037)
                     /gene="pspB"
                     /locus_tag="OQM34_003950"
     CDS             complement(1813..2037)
                     /gene="pspB"
                     /locus_tag="OQM34_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1454171.1"
                     /translation="MNALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2091..2759)
                     /gene="pspA"
                     /locus_tag="OQM34_003951"
     CDS             complement(2091..2759)
                     /gene="pspA"
                     /locus_tag="OQM34_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1454172.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2926..3903
                     /gene="pspF"
                     /locus_tag="OQM34_003952"
     CDS             2926..3903
                     /gene="pspF"
                     /locus_tag="OQM34_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1454173.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4023..>4328)
                     /locus_tag="OQM34_003953"
     CDS             complement(4023..>4328)
                     /locus_tag="OQM34_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1454174.1"
                     /translation="ERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAAEFNDPQTG
                     EPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDALSD"
BASE COUNT         1073 a         1081 c         1030 g         1144 t
ORIGIN      
        1 acacttcagg gctacggtaa ttcaggtcaa tttgatcgtc actaaaggtg gtccataaat
       61 ggcgcgtgga attatcatcc atttgaaacg gcgttaatag cggtaacgca cgcggaaggg
      121 tgacggcact gagatcggtt ttcggatcaa ccgcaataaa gaaatcttca aaacccggaa
      181 gctgttgtaa atagttttta aaccattcac tttttgcaga catatggttg cagacaaaat
      241 caaacattaa atgactgcat tcaccgagtt gctgaatatc ctgccactcc cccgcttcac
      301 tggcgacctg atgataatca attaccgaaa atccatcatc agatgaccat gggtaaaaag
      361 gtaataaatg aacatgtgaa aaaatgcttt gcagccattg atggtagaac tgattaaata
      421 tgggtagtgg ttttaaatca ttactgtgaa attgatcggc ataggtaatt aacacgacat
      481 cactttcatc ccagtgtttt ttacgtcgct gtgtaattaa tcgtttcgca ctctcaagac
      541 gcgttacaag tttctgtaaa tgagttgcgg taaatgttcc accgtagatt tcgtccatgt
      601 aatccgtaat tttctgtttc atgttgtctt tccgtggtag cggtcccatg aggcgagaga
      661 ctactccgca gaaaataaac tgtcaattga gcacaagggt tttagtcacg caggaaatca
      721 ttttttacca gggaaaaagc gtatttgtga gctgacgcaa ataatctata gggatgtaaa
      781 taaggtgtga cagaaaaaaa acggcgcata agcgccgctc atggtgaatt cttttaacct
      841 ttgaccttcg gcattgcgat gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc
      901 atctcgctaa ggagctcttt tgcctgcccg gactggcgtc cggcattgca atacactttc
      961 acggtgtcgt ttttatccgg aacggcggtg gcaatgcgct ctttcacttc cttcagggga
     1021 atattgatgg ccccctgaac gtgctcttgc tgatactgct ctggaacacg aacatcgatc
     1081 cagtgttcag cggcgaaaac gggcagtgaa aacaccagtg ccagagcaag taagcctttt
     1141 tttaaacatg taagcgtcct tctatggatg aaaattgcca tccattatac gcctgagatt
     1201 taacaatttt tgcgaaactc ccctcacctt ttataacgct gtgccagttt attagcagcc
     1261 cgacttaaca gcggttccag cgccacagcc agcaacattt tcagcggtcg gcgagcaact
     1321 gattttatcg cccagcccgc cacacccgcc gggccatagc gcagtgcggt aagaagtacc
     1381 agcttgcctg ctaatttgaa accaagcttc accttttgcc cggcctgttg ccagcgagta
     1441 ttcataactt tcctcacagt tgacggaaac ggctacgtaa cgtgaaagta tcggaagtga
     1501 cataacgttc catctcgcgt aaacgcgttt cacttgccgc cagttcacgg tcgacttcat
     1561 ccagcaattc gctgctggaa ggtagctgct caccaaaggc catgttgtcc ggcattggat
     1621 caagcgcaaa tgacaaaatg atgtaagcaa ccagggtaaa cagcgccaga ccgaagaaaa
     1681 tcgacagcac caccaggata cgcaccagtt ttaccggtac atcaaaatag ttggcaatcc
     1741 cggcgcagac gccgcggacc atgccctgct gtggaatacg ccataacttt ttattgagat
     1801 taatgcccgc cattagcgat ccctccagtt cggatgttct gcatcaagaa tagattccag
     1861 cgcctgaata cgttcgcgca tccgttttgc ttcatcaacc agttgcgcta atcgctgctg
     1921 ctcactttgc gacaattcac tgcgaccaga acgattgctg taatgcagcc ataaccagat
     1981 cggtaaaaca aacagcacaa aaatggttaa cggaatagcc agaaatagcg cgttcataag
     2041 tactccttag acgatgagcg gcggcgcgtt cggacgccgc ctggatgtta ttattgattg
     2101 tcttgcttca ttttggcttt taattgtgcc agttgttcgc tgattgcatc atcggctttc
     2161 agttcggcaa actgatcgtc cagcgatttt tgtttaccga agctgtggct ttctgcttcc
     2221 gcttccatct ggtcaatacg acgttcgaaa gattcgaaac gagccattgc ttcatccagt
     2281 ttgccactgt ccagctgacg acgcacatcg cgcgacgagt ttgccgcctg atgacgtaac
     2341 atcaatgcct gctggcgagc gcgtgtttcg ctcaatttgt tttccagctc gccaatctct
     2401 ttcttcatgc gtgccagcgt atcgtccacc agcgtcactt catgttccag ggacttaatc
     2461 agatcggtca gtttctgttt ttcaattaac gctgcacgtg ccagatcctc tctctctttc
     2521 agcagcgcca gttcggcttt ttcctgccat tcaacttcac gcgccgacgc ttgttcaata
     2581 cggcgagtca gctgtttctt ttctgccaac gcacgcgccg aagtagaacg tacttcaacc
     2641 agtgtatctt ccatctcctg gatcatcaga cgcaccagtt tctgtggatc ttccgctttc
     2701 tctaacagag cgttgatgtt ggcattcacg atgtcggcaa agcgagaaaa aatacccata
     2761 atgttgtcct cttgatttct gcgttcagga ttgtcctgct gaactgttaa tacaatttgc
     2821 gtgccaattt tttatctttt tgatttataa agatctgatt gaagaatcaa cagcaacatg
     2881 ccaggatgaa ttagcgaatt acactaacaa gtggcgaatt tcatcatggc cgaatacaaa
     2941 gataatttac ttggtgaggc gaacagcttt ctcgaagtgc tggaacaggt ttcgcatctc
     3001 gcaccgctgg acaaaccggt gctcatcatc ggcgaacgcg gcaccggtaa agagttgatt
     3061 gccagccgcc tgcattatct ctcctcccgt tggcaagggc cgtttatttc ccttaactgc
     3121 gcggcgttaa atgaaaatct gctggattcc gaactgtttg gtcacgaagc gggggcgttt
     3181 accggtgcgc aaaaacgtca tcctgggaga tttgaacgtg ccgacggcgg tacgctattt
     3241 cttgatgaac tcgctacggc accgatgatg gtgcaggaaa aattattgcg tgtgattgag
     3301 tacggtgaac tggagcgcgt tggcggtagc cagccgttgc aggtgaatgt gcggttggta
     3361 tgcgcgacga atgccgatct cccggcgatg gtcaatgaag gtacttttcg cgctgacctg
     3421 ctcgaccggc tggctttcga tgtagtgcaa ctgccgcccc tgcgcgagcg cgaaagcgac
     3481 attatgctga tggcagaaca ctttgccatt cagatgtgtc gggaaatcaa gctgcctctg
     3541 ttcccggggt ttacggagcg cgccagagaa acattgctga attatcgttg gccgggaaat
     3601 attcgtgaat tgaaaaacgt ggtggaacgt tcagtgtatc gccacggcac cagcgattat
     3661 ccgcttgatg acatcattat tgatcccttt aaacggcgtc cacctgaaga agccatcgcc
     3721 gtttcagaaa atacctcgct tccgacgctg ccgctggatt tacgcgagtt tcagatgcag
     3781 caggaaaaag agttgctgca actcagtttg caacagggga aatataacca gaagcgcgcg
     3841 gctgaattac tggggttaac ttatcatcag ttccgcgcgt tgttgaaaaa gcaccagatt
     3901 tagcgaagtt gagcaataca acttgccgga tgcggcgtga atgctttatt cggcatacat
     3961 aatcaatacg tcgtttgcag gcctgataag cgcagcgcat cagacattat tgcgttgggc
     4021 tattaatcgc tcagcgcatc ctgcaaaatt ttcattgccg catcgaattg cgcatccggg
     4081 atggtcagcg gatacaggaa gcgaatcacg ttgccgtatg cgccacaggt cagcaggagc
     4141 agcccctgcg ccagcgcgcg ttgctggatt ttctgtgcaa tcgccgctga cggctcgccc
     4201 gtttgcggat cgttaaactc tgccgcaatc atcgacccca ggccgcgtac cgcagcaatg
     4261 gccggaacgc tttctttggc atcaatcaac gtgtttttga gacgctggcc cagttgattc
     4321 gcgcgttc
//
LOCUS       DAJVPP010000193         4209 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVPP010000193 DAJVPP010000000
VERSION     DAJVPP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.196"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM34_003954"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM34_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1454175.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OQM34_003955"
     tRNA            complement(324..399)
                     /locus_tag="OQM34_003955"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OQM34_003956"
     tRNA            complement(406..480)
                     /locus_tag="OQM34_003956"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OQM34_003957"
     tRNA            complement(597..681)
                     /locus_tag="OQM34_003957"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OQM34_003958"
     tRNA            complement(690..765)
                     /locus_tag="OQM34_003958"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OQM34_003959"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OQM34_003959"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1454176.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQM34_003960"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQM34_003960"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1454177.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQM34_003961"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQM34_003961"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1454178.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1047 c          937 g         1136 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgc gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJVPP010000194         4087 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVPP010000194 DAJVPP010000000
VERSION     DAJVPP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.197"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(109..966)
                     /gene="sitD"
                     /locus_tag="OQM34_003962"
     CDS             complement(109..966)
                     /gene="sitD"
                     /locus_tag="OQM34_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1454179.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(963..1820)
                     /gene="sitC"
                     /locus_tag="OQM34_003963"
     CDS             complement(963..1820)
                     /gene="sitC"
                     /locus_tag="OQM34_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1454180.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1817..2644)
                     /gene="sitB"
                     /locus_tag="OQM34_003964"
     CDS             complement(1817..2644)
                     /gene="sitB"
                     /locus_tag="OQM34_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1454181.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2644..3558)
                     /gene="sitA"
                     /locus_tag="OQM34_003965"
     CDS             complement(2644..3558)
                     /gene="sitA"
                     /locus_tag="OQM34_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1454182.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1113 a         1001 c          970 g         1003 t
ORIGIN      
        1 gattttacgt attttttgca tgatagaatt cgtacctcct cccaacgacc ttccatggct
       61 ttccggactt ctgtaggctg gcggacgatg ctgtcctcgg atgttgtgct aattgggtga
      121 taacagatcc tgtgtatggg cgtttccctt tgtgcgtgcg ttgatgctgg taacggtaaa
      181 cgtcatgatg aacacgaccg cgaataagac gacaatcgtc ggagctggtg cgctgtcgat
      241 aaaaaaggac agccagacac cagtcattga cactattacc gatatgccag tcgccagtag
      301 gagcgcaata tgaaagcgtt gtgtcaggag tactgcaatg gcgccaggcg caatgagcag
      361 agaaatagag agaatgatac ctactgcttt cagcgtcgcc acaatggtca gggaaaccat
      421 gcacagcagc ccataatgca gccatctcgt atgcaaaccg cttacctgcg cctgttgata
      481 atcaaagctg aacagcaaaa aatcccgcca ttttacgcta ataacaagtg taaccagccc
      541 ggcaataatc accgtctgga ttatatcgcc gatggttatc ccgagcatgt cgccgaaaag
      601 aatatggtca agatgtacgt ctggtttaat tgcaatatac aagatcagac ctgcggcaaa
      661 catgccagaa aaaacaatcc ccataactgt atcttgcttg attcggctgt tgtctttcag
      721 gtatcccgtc gccaccgcgc agaatactcc ggcgacgaat gcccctgtag ccagaggtaa
      781 ccccagaatc caggtaagca cgatgccagg aaaaaccgca tgactcatcg cgtcacccat
      841 cagtgcccaa cctttcagga ccagaaagac cgacagcagc gcacagggta tggaaactac
      901 cagtgaaatc agcagtgcgt gactcataaa ggtgaactgc agtggctcca gtaataatgc
      961 catcatgagc gatcctccag cgctttatgc gcacgccgac gatttgccaa aagtccatgg
     1021 gtgggcgcaa atacaaatgc taaaagaaac agcagtgtct gcgccaccac gatgattcca
     1081 cctgtcgcgc cgtccagaaa gtaactgacc cacgcgccga ggaaactggt tacactgcca
     1141 atagttacag caattatgag caggcgggga aaacgatcgg ttaacaacca tgctgttgca
     1201 ccgggagtca ccaccagaca gatcaccaga aaggcaccaa ccgtttgcag cgccgcaacg
     1261 gtcgaaaccg acagcagggt gaagaagagg atttttaatc tgcccggatg taatccaata
     1321 gcacgggcgt gattctcgtc gaaaaaggtc accataagat ctttccattt gaaaaaaagc
     1381 acgatgatcg agagaatgcc aatgatagtg agttgcagga tatccgccgg atcgattgcc
     1441 agaatattgc ccaggacgat ggtctgaatg ttcacggatg tcgggttcaa tgacaccata
     1501 aacagcccca ggccaaaaaa ggacgagaag atcaggccga tgatggcatc ttcttttaag
     1561 cgggtgcgtt ggttaagaaa cagcatgctg cccgctgcca gtccgccgga aaagaaggct
     1621 cccagagaaa acggaagccc cagcatatat gcccccgcaa cacccgggac aatagagtgc
     1681 gacagcgcat cgccaatcag cgaccagcct tttagcatca gatagcatga caggaaagcg
     1741 cagagaccgc cgaccattgc cgagacccac atcgcattga gcatatattc atagctgaag
     1801 ggttccagta gcacgttcat cttgcttccc tctgcaccgc tgacggtcga tgtgccacaa
     1861 aagggcgttc atcgtcagta ataatgcttt cttccgagcc gttaagtgtg acatggcgca
     1921 gtacgccgct aaaagccagc tccaggttct cggctgtaaa agtggtgtct gtcggcccgc
     1981 ttgccaggac ggtgcctttg accatgaccg tataatcgca aaatgtcgtg actgagccga
     2041 gattgtgcgt tgagacaagc atcgttttgc cttccgcgcg taactccctc aacaggctga
     2101 tgattttcgc ttcggttttc acatcaacgc cggtaaaagg ttcatccagc aggatcacat
     2161 caccctgctg tgcaattgct ctcgccagaa agacgcgttt cttttgtcca ccggaaagct
     2221 cgccgatttg acgatggcga aaatccacca tatcgacacg ttccagcgca tcggtgacaa
     2281 tctgacgatc cctctttttg gctatacgca acatacccat atgcccatag cggcccatca
     2341 tcaccacatc ttccaccagt acaggaaatg accagtcaac ctcttctgac tgagggacgt
     2401 aggcaaccag gtttttctgt agcgcctgtc gcgtgggaat acccagaaca gatatctttc
     2461 cgctggtcag acgcacaaat cccataatcg ctttaaacaa cgtcgatttt ccggaaccgt
     2521 ttaccccaac cagagcggca atagatccac ttggtactgt gaaggatgca tcgcgcagtg
     2581 ctgtgtgccc gttacgccag gtgacagtaa catcattaac gacaatgcct gcagattgca
     2641 tcattatttc tcccttttcc cggctttaat tccctgtacc agcgtactgg tagtaacctt
     2701 aagaaggtcg atatacgtag gtaccgggcc gttttctgtg ctcagggaat cgacatagag
     2761 cacaccgccg tagtgtgcgc cggtttcacg cgcaacctga cgcgctggtt tatcggaaat
     2821 cgtactctcg ctaaagactg ccgggatatg atttttctta actatatcaa caaccttacg
     2881 tacctgctgc ggtgttcctt gttgatcggc attaatcggc cacagataaa gctcttttag
     2941 ccccaaatcg cgtgcgagat aagaaaaagc cccttcactg gtgaccatcc atcgctgatt
     3001 ctcagggagt tccgtaatct gcttacgcag gggggcaagg gtttgggtaa tcttggcttt
     3061 ataagtatcg gcattacgtt ggtaggtttg tgcatttgcc ggatcgtatt ttatcaacgc
     3121 atcacgaata ttatcgacgt aaatcagagc attatctggc gacatccatg catggggatt
     3181 aggtttgccc tcatagggtc cttcggtgat ccctactggc gtcacacccg aagagacaat
     3241 tacttctgga accccattga gatgctggta aaagcgttgg aaccacaatt ccagattcat
     3301 accattggcg agaatcagtt gtgccccctg cgcacgttta atatcgccag gggtaggctg
     3361 atactcatga atttctgcac caggcttggt tatggatgag acttctgcag catctccagc
     3421 cacgtttttg gccatatccg cgatgatggt aaatgttgta atgaccttga atttttcagt
     3481 tgcacttgcc tgaaatgcga tcgagcaggt gagggcgagg caccccaaga gcatggttac
     3541 tttttttatt gagtgcataa tttagtacct atagtgagca aaattagaac aaacatagca
     3601 acggctatgt tttatagcat aacctataca aaatgaaaat aattatcatt aacagctttg
     3661 aggcctaaaa cagggattag gttgtttcct gctggcgaca tgatattgag gaaaaaagta
     3721 gagcagctat taggctttga aatgacttta tgaggaaaat tgttgatggc aaaaatgggg
     3781 caaaacgctg tataaggggc aaaaaagggg caaaaaagag tgggttatcg tagcttattg
     3841 ttgtcgctga taatgtttaa catattgaaa aataaataaa atacttatga gtcagatagt
     3901 tgtgattttt gcccttactt gttcaggttg tattgttctt tctttgtaat ttgttgattt
     3961 tcttgcatta tttcagttct ctggtactaa atggggcaaa ttgggggcaa actttgcaac
     4021 tacgataacc gcgcattcaa catggctatc tgttcgtcgt tcatgtcatc aatccacata
     4081 ccgtaaa
//
LOCUS       DAJVPP010000195         4059 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVPP010000195 DAJVPP010000000
VERSION     DAJVPP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.198"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(903..1094)
                     /locus_tag="OQM34_003966"
     CDS             complement(903..1094)
                     /locus_tag="OQM34_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1454183.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1158..1481
                     /locus_tag="OQM34_003967"
     CDS             1158..1481
                     /locus_tag="OQM34_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCS1454184.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1471..1782
                     /locus_tag="OQM34_003968"
     CDS             1471..1782
                     /locus_tag="OQM34_003968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454185.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1757..2275
                     /locus_tag="OQM34_003969"
     CDS             1757..2275
                     /locus_tag="OQM34_003969"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCS1454186.1"
                     /translation="MHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLV
                     NHHYGLHDPNAPENRRTLTLPDNLPETKQAIAEGVTRGIDALYHAGEIKGRQDVIQAL
                     TEAGIDVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGCGVREKAERESRIYRENA
                     ERRVQEARRICK"
     gene            2160..2690
                     /locus_tag="OQM34_003970"
     CDS             2160..2690
                     /locus_tag="OQM34_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCS1454187.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2697..2930
                     /locus_tag="OQM34_003971"
     CDS             2697..2930
                     /locus_tag="OQM34_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1454188.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3476..3943)
                     /locus_tag="OQM34_003972"
     CDS             complement(3476..3943)
                     /locus_tag="OQM34_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454189.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          992 a          923 c         1178 g          966 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgccgg gagcagcata
      901 tgtcagaggt tttcaccgtc atccccgaaa cgtgcgaggc agctgcggta aagctcatcg
      961 gcgtggtcgt gaaacgattc acagatatca gcctgttcat ccgcgtccag ttcgttgagc
     1021 ttctccagca gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     1081 tcctgtttag tcactgatgc ctccgtgtaa gggggatttc tgttcaggag gtaatgatac
     1141 cgatgaaacg cgagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     1201 tggaacgctg tgagggtaaa cagctggcgg tatggatgcg gcgggtctgc ctgggggagc
     1261 cggttgcccg ttccgggaaa ctgccgacac tggcaccgcc gttactgtgt cagctggctg
     1321 ccatcgggaa taacctgaat cagacagcga ggaaggtgaa cagcgggcag tggtcttccg
     1381 gtaaccgggt tcaggtggtg gccgcactga tggccatcgg ggacgagctg cgccggctgc
     1441 gtctggccgt cagggaacag gggacgcggg atgatagtta agtttcatgc ccggggaaaa
     1501 ggtggtggca gtggtccggt tgattacctg ctgggaaggg agcgtaaccg ggaaggggca
     1561 acggtgcttc agggtaatcc ggaagaagtc cgggaactca tcgatgccac gccatttgcg
     1621 aagaaataca cgtccggtgt tctgtcgttc gcggagaagg agctgccgcc gggaggacgt
     1681 gaaaaagtga tggcgagctt tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac
     1741 agcatcctgt gggtggatgc accaggacaa gggacggctt gagctgaatt ttgtcattcc
     1801 gaacatggag ctacagaccg gaaaacgcct ccagccgtac tacgaccgcg cagacaggcc
     1861 tagaattgat gcctggcaga cgctggttaa tcaccattac gggctgcatg acccgaacgc
     1921 cccggagaac cgcaggacgc tgacactccc tgataatctg ccggaaacga aacaggcgat
     1981 tgctgagggc gttacacgcg ggatagacgc gctttaccat gccggagaga taaaaggccg
     2041 tcaggatgtg attcaggctc tcacagaggc agggattgac gtggttaggg tgacgcgaag
     2101 cagtatcagc attgcagacc cgaacggcgg gaagaatatc agactgaaag gagcgtttta
     2161 tgagcaatct tttacagacg ggtgcggagt tcgagaaaaa gctgaaagag agagccgaat
     2221 ctacagagaa aatgctgaac gacgagttca ggaagctcga agaatctgta aatagggaac
     2281 tgacctcaaa cgaatcttta atcaggaacg ctatcaacga ccatacatca gccctgaaag
     2341 aacttctgga gaggtatcag aaaacgaccg tcgatacgat ggacgcacac tggaaaaccg
     2401 tactgaaaat gagcgtgaaa cgctggctgt ggctgattat cgtctcagta ctgatgtttg
     2461 ccacgaccgg gagcctgctg tggtaccagg gcatgaagat aaacgcgaac atgaatattc
     2521 tccgcgagca gaaagagagt ctggagaagc tgaacgcgaa gacatggggc gtgcgttatc
     2581 acgaggacag caacggacgt tttctggtgt tgcccgaggg gatgaaagcc gaaacgaact
     2641 ggacgatgga aaacgggaaa cagaatgccg taaggctggt acaggagtaa cagagcatga
     2701 cggagctgga aagacatttg ctgaacgcat tagaacaact gcaacaggac tatatgcagc
     2761 ggctgaacga atgggagagc gccttcgtgg aattgcagaa aatgttttcg cttacgcaac
     2821 gggacaacgc gatgctgaac gagcgggtca tgcagttgag tcagcaggtg cagcacttga
     2881 gcgagcagac agaacgcttg agccagttat acagcgagaa caggagataa gggacgaacg
     2941 gctgatacag gaatgcgaac atgcgttatc cctggagcgt gagcaccagc tggaaataca
     3001 ggaacggaca ctggatggcc cttcgctggg atggtaatca gagacaggct aaaatgttgt
     3061 cagcgcgttt ccggtgcatt ttttgggata atggttggtt cgggggcacc gggaacgcgc
     3121 ttcagtcaga ccttgctgaa aacataggaa cgcgttaaaa cgcatacggg cgcgattctg
     3181 gtgtgtcttt tgttgctgtg ttttgcctct attgccagat cgcgctcatt cccctgaact
     3241 tcttttttcc cgctcaggtc cccgtcttct tcgcttccga aagcatgaaa aaaatccgtt
     3301 tcagctgtct ctggctggca attcctgcgt gattcacatg gctgcatagc tatgcatgtg
     3361 gttaaaattt accgggtgcg atcgcggcag tttttcgggt ggtttgttgc catttttacc
     3421 tgtctgctgc cgtgatcgcg ccgaacgcgt ttcagcggtg cgtacaatta agatattacg
     3481 ttaaattgtg agggaatggt ccccatatac tgactttatc atagggatag ggttggtagt
     3541 gggtcgattg ggtttcggca ttgtttccgt taatgccaat tgcatttttg tatttgatgc
     3601 aacagaaggc gtgagattct cctgggcctg gaaaggataa gtgtttagaa ttgatggcaa
     3661 aatatcttcc atccgatttt tgaagtagtt gttgtttggc tgtctcccaa agggtgttgt
     3721 tacaaacttc ttgaatttgt agtttttgca ggactcttat tactgcgtta tcatgttcca
     3781 attctttagg ggagtgtatt attctctctt ttaataagct gtttaccaca gggtttagtt
     3841 gttgtctaaa gcacgcagat aatgccattg ctacacaatt ttgttcccga ggaagcatgt
     3901 ttggcggggt atgccgtgtt tgatgtgata tgtctattct catagaaggt ctctggctta
     3961 tagcatcaac aaatacggct ataaaacaaa attttcatag agtgattcaa gatcttcttg
     4021 agatcctttt ttgcggcgcg taatctgctg cctgcaaac
//
LOCUS       DAJVPP010000196         3961 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVPP010000196 DAJVPP010000000
VERSION     DAJVPP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.199"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..117)
                     /locus_tag="OQM34_003973"
     CDS             complement(<1..117)
                     /locus_tag="OQM34_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454190.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGG"
     gene            complement(362..892)
                     /gene="cybB"
                     /locus_tag="OQM34_003974"
     CDS             complement(362..892)
                     /gene="cybB"
                     /locus_tag="OQM34_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS1454191.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1081..2082
                     /gene="gap"
                     /locus_tag="OQM34_003975"
     CDS             1081..2082
                     /gene="gap"
                     /locus_tag="OQM34_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1454192.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(2124..3563)
                     /gene="aldA"
                     /locus_tag="OQM34_003976"
     CDS             complement(2124..3563)
                     /gene="aldA"
                     /locus_tag="OQM34_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCS1454193.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3760..>3961)
                     /locus_tag="OQM34_003977"
     CDS             complement(3760..>3961)
                     /locus_tag="OQM34_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCS1454194.1"
                     /translation="GLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVI
                     HAWQTLKHDAVLIEAMESRSLR"
BASE COUNT         1059 a          998 c          904 g         1000 t
ORIGIN      
        1 gccgcctgcc gtacagtgtg agatcccccc tttcgaacca gaacagggtt tgcgcccggc
       61 atcggcataa aaggaaacaa gcgttcccat aaacaaaata agtgccagtt ttttcatata
      121 acctccatgg tttatcgaat cttatgtgcg atttgaacgt tatacgctgg tttattttga
      181 taccgttgat gagatcaaag atcgggtgaa ttgattaaaa ataagccaga tggacgtatg
      241 ggcaaggatt atggttttat ttgtcataca aataaatata ataggcgctt ccgatgtaga
      301 cccgtattct tcgcctgtac cacgggtcgg ttttagtaca ggcgttttct ttaaatatcc
      361 ttcaggaacg tttacgcggc atcatgcgta gaagtgtgtt gtccttccag aaatagtggt
      421 gtgccagtgc agccgcagcg tgcaacccga tgacaaaata tcccagattc gccagcgtct
      481 catgccacga ctttaagcta tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg
      541 tcaaaccaaa cgcaaaccac gggttgcccc ggttatacat catcaccaaa ccaatcactg
      601 gcagcgcaat aaacaggaga taaatcacca aatgtcccaa atgcgccagt cccgtcatca
      661 tcggtttcgg cttaggtata atcggcgggg ttgggtattt cagtcttaac aaaagacgaa
      721 ccaccatcag cacgagaatt gagatgccac aggaaacatg aatcatgttg atgagcggcc
      781 gatcgctacg tgggaagaaa ccacgaaact ccattgcgca atacgctgcg ataaccagca
      841 aaaagaccag ccagtgaatg ctgatttgta accttgagta tttattttcc ataacatttc
      901 ctgctttaac ataattttcc gttaacataa cgggcttttc tcaaaatttc attaaatatt
      961 gttcacccgt tttcagtacg ttacgtttca ctgtaagaat ggagattgcc catcccacaa
     1021 tcctggtcta agcctggaaa ggatcaattt tcatccgaac gttcctgaca ggagaaaacc
     1081 atgagtaaag ttggtattaa cggttttggt cgtatcggtc gactggtgtt gcgtcgttta
     1141 cttgaagtca aaagcaacat agacgttgtc gctattaatg atcttacttc cccaaaaatt
     1201 ctcgcctatc tgctgaaaca tgattcaaac tatggtccgt tcccctggag cgttgatttt
     1261 acggaagatt cacttatcgt tgatgggaaa agtatcgcgg tttacgccga aaaagaggct
     1321 aaaaatattc cgtggaaagc gaaaggggca gaaatcattg tcgaatgtac tggcttttat
     1381 acctccgccg ataaatcgca ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc
     1441 gctcctgccg gtgaaatgaa aaccatcgtt tataacgtta atgacgacac tctggatggc
     1501 aacgacacca ttgtttccgt ggcgtcatgc accactaact gccttgcgcc gatggccaaa
     1561 gccttgcatg acagtttcgg gatagaagtc ggcacgatga cgaccattca tgcctatact
     1621 ggcacccagt cactggtgga tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg
     1681 gcagaaaata tcattcccca cactacgggg gcggcaaaag ccattggtct ggtgatcccg
     1741 gaactgagcg gcaaactgaa aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc
     1801 actgagctgg tgtccattct cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt
     1861 aaacaagcaa ccaccaataa cgagtcattt ggttataccg atgaagaaat agtctcttcc
     1921 gatatcattg gcagccattt cggttcagtg tttgatgcca cgcaaacgga aattaccgcc
     1981 gtgggcgatt tacaactggt gaaaacggtc gcctggtacg ataacgaata tggcttcgtc
     2041 acgcagctta ttcgcaccct cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa
     2101 aaaacctccg cctctttcac tcattaagac tgtaaataaa ccacctgggt ctgcagatat
     2161 tcatgcaggc catgtttacc atctgcgccg ccaataccgg atttacgcca tccggcgtgg
     2221 aaaccttgca tagcttcgaa gttttcacgg ttgatgtaag tttcaccaaa cttcagccct
     2281 ttaatggctt tcatcgcgac gttcagattt tgggtataga ttgatgaggt caggccgtaa
     2341 tcactgtcat tagccattga gatagcatct tccagcgtgt caaatgcgac aactggcagc
     2401 accgggccaa aggtttcctc atgcataatc gacatttcct ggcgaacatc cagcagcaat
     2461 gtcggcggat aataatatcc tttcccctct accgctttgc cacccaacgc cactctcgcc
     2521 ccttcttcta ctgcgcgcgc cactttttgc tcaacccttt ccagcgccgc ggcgttaatc
     2581 aacggcccca tcgcaatgtc gttgcgttca gcggggttac caaattgaac cgcctgcaac
     2641 gcttcaccca gcagattgac gaactgatca taaatgcctt tctgtacata aacacgttct
     2701 gcacagttac acacttgccc actattaatg acgcgtgaat caacgatggc tttgactgcc
     2761 agttcaagat cggcatcgtc cattacgata gccggtgctt taccacccaa ttccagacac
     2821 actttggtga tgtttttcgc cgcagtcgcc atgatcttct cacctgcaga gacgctgcct
     2881 gtcatactga ccattgcgac ctttgggtta cccgccagtt cttgcccaac ggtttcacca
     2941 cgccccagta caaggttaaa cacgccgcgc ggaaggccta tttcatcgac gattttggcg
     3001 aatgcaatcg cattgtttgg cgtaaattca ctgggtttaa tgacgatggt attaccggtc
     3061 aaaagagcgg gagccatttt gcgggcaatg aggaagaacg gaaagttcca cggcagaatg
     3121 ccggtagtca caccaagcgc acgtttaaac agaagaatat tttctcccgg acgatcgctt
     3181 tgaataatct cgccctcgta acgccgtgcc cactccgcca tgtaatcgat atagtcggca
     3241 gtaaaagcca cttcgacttc agctagctgc tggatcttgc ccccttcttc aacaatcagc
     3301 gcactgattt cactggcgcg ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg
     3361 cgttcaatag caggcaacgc ttcccattct ggttgtgcac gttctgctgc atcgattgcc
     3421 ttacgggcat cctcggcctg accatcgggt atgcgggaaa tgacagcctc tgtagcaggg
     3481 tttaccacat caatccatgc gtctccacgc caggtaacaa actgtccatc gatatacata
     3541 ggatgttgaa cgggtactga catgggcgac tcttgtgatt tatatgtttg ttttcggtga
     3601 atatattgtt aattaacatc aatcatcaga gagacggcat cgggaaagga caagtctgtg
     3661 gagggcttca taaaaacaag tgtaaaagga ataaaaggca gaaataaaag tcaggagtca
     3721 tgcccgtttg caaacaagtg gttcacaaac gggcaatttt taacgtaacg agcgactttc
     3781 catcgcctcg atgagcaccg catcatgttt cagcgtttgc catgcatgga tgacttctgc
     3841 cggaaaatca acgtgaacga taaaatctcg cccgcgggga ccgtagccat cgctatcatc
     3901 gtgtaaacgc ggcagctcgc cagtgagtag tgagagataa cgctcaaccg gccataatcc
     3961 t
//
LOCUS       DAJVPP010000197         3943 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVPP010000197 DAJVPP010000000
VERSION     DAJVPP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.200"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            12..920
                     /locus_tag="OQM34_003978"
     CDS             12..920
                     /locus_tag="OQM34_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS1454195.1"
                     /translation="MPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEA
                     TFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKM
                     TNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVR
                     IPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLT
                     QAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGD
                     YLTAEKIRKRAARVFVNDERFTRGGN"
     gene            920..1594
                     /locus_tag="OQM34_003979"
     CDS             920..1594
                     /locus_tag="OQM34_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1454196.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2122..2883
                     /locus_tag="OQM34_003980"
     CDS             2122..2883
                     /locus_tag="OQM34_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1454197.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            2927..>3943
                     /locus_tag="OQM34_003981"
     CDS             2927..>3943
                     /locus_tag="OQM34_003981"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012786.1,HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1454198.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAI"
BASE COUNT         1127 a          733 c          959 g         1124 t
ORIGIN      
        1 caaactatgc catgccaggg gagacaacgg ctgactttac gcattcatca tcgaatgacc
       61 agcgtatacg tattctgaaa ggtgccataa gtcttattca acagcgcccc ttaaccggtt
      121 gggggttagg cagctttgaa gcgaccttcc cgcaagcctt agaggatgtg ggcgtgacaa
      181 acatagagag tgatacggct acgcatcccc ataacgaact gctgttcgta acggctgaag
      241 gtggcatcgt cgccttgttt ggttttttgc tgctatcagg cgtttggctg tggccgtgta
      301 tatgccgcct gaaaatgaca aacaataacg cgcaccatca atgtgaaaag tggtttattg
      361 ccttaatggg gttgccttta gtcattcata tgatgaccga atatcctctc tatacgtctg
      421 ttccccattt gatgctgttg ctgctccttt tccgcatcgg aatgccggaa aacgtgatga
      481 atcaggtacg tatacctaaa acagtccggt tgggtattct gcctgtcatt gtgctcatta
      541 tgaccgccgc gctggtcatc ctggtagcgg gatttgatgc acagcgggaa ctgaccagag
      601 ctgaagctga tatgaacgaa ggatttatcc ctcgccttcc ggaacatagc tggaaatacc
      661 tgacgcaggc agaacgtctt gagcgcgatc agcatctgct tattgcgaat aaaccggggt
      721 tcgttcgcga tacggaaagt atgtctcttt tcactgtgtg gggaaataaa tggttggcaa
      781 tccacaatga tgcagacgta agcgctgcaa tgatattcat tgcgcacagg cgcggtgatt
      841 atctgacagc tgaaaaaata agaaaacgcg cagctcgggt atttgtaaat gacgaacgtt
      901 ttactcgcgg gggaaactaa tggttaacga tctgacgtat aagaaattag aaagcacaaa
      961 catcaactgt aatattctct cgccagaaaa taccacatgg acaaatgtca cggtgattgg
     1021 cctggatctt tttgccattt gcggcgtgtg cagtttgctc aagcgtgttg ggggagtgaa
     1081 acttcaggtt ttcacttcct tacccactga tatgaatgtt ctgactgaca gtagcatcgt
     1141 gatatgggta aaaatgcgtc acgatggttt gcctgaatta gccggacata ttgtgaaggt
     1201 ttgtcgtcga taccgtctga tgcggcaact cgttattagc gatgctattc ctgaaggaat
     1261 gacgaatgat atcgggcctc tttcccgagt atggcttact catgggagtg caaaagtgga
     1321 tattttatta tcttcattac ggaaggtgtt aaatgctcat tgtttgcctg gtcctttgtt
     1381 tataaaacgc ctgggaagag tgcaatggag ggttctttta ctccgcgcga aggggattag
     1441 cacacgtaaa attgcagaaa tctgtggtat tggcctcaag accgttagcg cccatgagtc
     1501 cgcaattcga gaacgattag gcaccaggaa taaaagtgag tacgtctggt tagtacgtaa
     1561 tgctcaactt atacaacagg cgatacccgc gtagggaaga gatgcgtcaa ggtatgaaag
     1621 atgtaacaat atgcgaacaa agcaaaaagt ataattgatg cgtcatagat aactctcttt
     1681 ttcgatgacg aaatcgctga gcatgcgtgg tgaggttagt aatagatggg tgctatgttt
     1741 tgccacgggt ttgtagctgt gaaacctgga aaaaaataag aattaaaaat atttaaagag
     1801 agttaataag gttctggttg taattgtctt ggtcaaaaaa aagatattgt caatgttgtt
     1861 ttttaaattt tcaggttttg gttaaatatt tgcttacaaa tataggtgat ttttttattg
     1921 taggcttttg aaggctatta taactgtgcg ctaaattaat gcgttgaata aaagaaataa
     1981 caaaattaag aaaagccata tttacgatat gggcaacata aatataatct aaaaaagagt
     2041 aaggcgttgt aggttggttc cgcgttcatt accttgttag tgaacgcgga tttatcagtt
     2101 gtcgaaatgg tttttataat aatgaaaaat tttaatgtac taaccaaagt cgtattttgc
     2161 ttgtcgtttt tatcaagcgc atttcccacc tatgctgcgg atgatgtcaa agtgactcaa
     2221 aacacttcag tttttaaact gaagcttagt gcaacgcgat taatctatga acaagggtcg
     2281 gatggcgcag cgataactgt gacaaatccg caggattggc cgatgcttgt gcagagtaaa
     2341 gtctttgaag aagataaagt aactgcagcg ccctttattg tcactccgcc actgttcagg
     2401 cttaatccag gccagcaaaa taaaatgtgg gttgtgcaga ctgcgccagc tttacaagac
     2461 agggaaaaat tacactggct atgcgccacc ggcattacgc ctaaaaagag tgatgtttgg
     2521 gccgagaaag ataaagaaaa aattgctgaa aatgtgaacc tgaacatcca ggttaacgta
     2581 catacctgca taaaacttat taccaggcca tctggtattg aaggcaagat ggttaatgcg
     2641 agtgagaagt tatcgtggaa aaagcagggt gataaaattg tcgcggtcaa tcagtcgccg
     2701 ttctacatca acctttcttc tttacaggtt ggcggtagca agattgagaa actagattat
     2761 attgcgccgt tttcgcaaca tgagtttgtt atgccaaaaa ctagcggcca ggatattaaa
     2821 tggtctgtga ttactgatga aggtggtgag agtcgtttgt ttatatctca tttgcaatca
     2881 taatgtatta catttttatt gaatattttt atgccatgca tattccatgt tttatcgact
     2941 gtcattaatt ttgataatgg ccttacttgc gggtcagtta agtgcccagg agtggtcatt
     3001 tgattcttca cagttggaag gaaacgttag tgcggacact gtggcaatgt ttaatcaggg
     3061 agagcaactc cccggaaatt atcgtgtgga aatttatctt aacggtgaaa aagttgatgt
     3121 tggggagttt ccctttcatc gtccggaatc ccctgaagaa aaagagcttg ttccatgttt
     3181 aaccgttgat gaccttatcc actatggcat taaaattgat aaaagcagca gtgatactga
     3241 caataaaaag aatcagtgtt ttaaatggaa ctctattgaa ggtttgaaag taaattatga
     3301 ttttgatagc cagcgcgtac agataaccgt tccacaactg tatctgcaag ataaaaaaag
     3361 tagtttagca ccagtgtcgt tatggaatga gggtgtagct gcattccgca tggtgtatca
     3421 gacaaatatt gatatttcca aacaaaatga taatcaatct accacgcgta attctcgtta
     3481 tggcaggttc actccagggt ttaatcttgg tgcctggagg tttcgtagtt cagtgacatg
     3541 gagcaaggaa cttggccagt cagaaagatg gcaaaggggt tacatgtggt ttgagcgagg
     3601 cataaatgcg attaaaagcc gattaacgct tggagaatct tatacgtcgt ctgaggtgtt
     3661 tgacagcata ccttttcgcg gcgggatgtt agcgaccgac gatgcgatga ctccgccgga
     3721 ggacagctac tacacacctg tggtgcatgg aatagcacaa agtgaggcgc aggttattat
     3781 taaacaaaat gggcaaatta tttttacccg ttctgtgccg ccagggcctt ttgcgctgga
     3841 taatttacca acgctggccg ttggcgggga gcttgatgtt acagtgagag agagtaatgg
     3901 cgaggagcag tatttttccg tgccatttca aacgccggcc atc
//
LOCUS       DAJVPP010000198         3892 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVPP010000198 DAJVPP010000000
VERSION     DAJVPP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.202"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(2..163)
                     /locus_tag="OQM34_003982"
     CDS             complement(2..163)
                     /locus_tag="OQM34_003982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454199.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(160..348)
                     /locus_tag="OQM34_003983"
     CDS             complement(160..348)
                     /locus_tag="OQM34_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1454200.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            608..943
                     /gene="iraM"
                     /locus_tag="OQM34_003984"
     CDS             608..943
                     /gene="iraM"
                     /locus_tag="OQM34_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1454201.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1224..1332)
                     /locus_tag="OQM34_003985"
                     /pseudo
     CDS             complement(1224..1332)
                     /locus_tag="OQM34_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1855..1931)
                     /locus_tag="OQM34_003986"
     tRNA            complement(1855..1931)
                     /locus_tag="OQM34_003986"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1895..1897),aa:Arg,seq:tct)
     gene            complement(2033..2108)
                     /locus_tag="OQM34_003987"
     tRNA            complement(2033..2108)
                     /locus_tag="OQM34_003987"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2073..2075),aa:Ile,seq:cat)
     gene            complement(2253..3074)
                     /locus_tag="OQM34_003988"
     CDS             complement(2253..3074)
                     /locus_tag="OQM34_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1454202.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3089..3445)
                     /locus_tag="OQM34_003989"
     CDS             complement(3089..3445)
                     /locus_tag="OQM34_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1454203.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3458..>3892)
                     /locus_tag="OQM34_003990"
     CDS             complement(3458..>3892)
                     /locus_tag="OQM34_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1454204.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPES
                     PESFMKRPKRRRWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTL
                     PLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1082 a         1040 c          764 g         1006 t
ORIGIN      
        1 tttatgatga tgccggtgca tttatctcca gcaccagact ttctatctca acgccatacg
       61 ttgcattttt ggtaatatcc gtcagcgtca gtgcatttag tcccactgcc agactgtctt
      121 ttatggcctg gaatgccggg ccagtacgat gacgtagtat cactccggct cagttgcacc
      181 actgaccacc acatcacctt ctgctgcaat cgcctgcatc agggtataag gggttatggc
      241 caccggacta ccaaacggct gccagccctc tttcagttta tgtgtcagct tttccgcaag
      301 atctgacggc ggcgccgccc tgacaacatc atagtgttta aatgccatgg ttctttccaa
      361 catctgaaaa ataattcttt aaaatacctg acatgtaata cagaaaaaac acaaaaccat
      421 accttaaata aaaacctgat tatcaagcag atatgcatgg ataaactaca agacgagata
      481 taaaccaccc tgcatttaaa taaacaataa acaacatcag aaaaataatt ctgctctatg
      541 gtttaattca aaaatatcat ttatactttt cagaacatca ccagcaaggc ataaacaagg
      601 aaactaaatg aagtggattg tgattgatac agttatccag ccatcatgcg gaatatcttt
      661 ttcagtcata tggagtaaaa taaaattaat aatctggtat caatcggatg ctttcttacc
      721 tcctgaaagt atatttacac tgactcacac aggcatcatg ctcaataaca aagtgctacc
      781 tgtaaccatt tacaacgtag taccattcaa taaaacattc tggaatttaa tcaaaaacag
      841 ccaggaatgc cctacaaata cagataacgt attgaatgaa tgctttaata accgttgcac
      901 tctgcaaata tgtccttatg gactaaaaca acaaagtcca taaggagttt actcacatct
      961 gacaaaatca atataaacag cccctccgga gaggggctgg agagtggcgc tatgtgccat
     1021 tccatggtgc cgggtgcctc ccggtgaatt cagtaccagc acctgaatcc gcgattatcc
     1081 catataccta ctcgctgatt gcccctccgc acagggggat tcaccatgcc agtttctttt
     1141 aacaaactcc ccgcaaacca gacaacagtc aaccgcctga attgtgaagt atttaaaaat
     1201 ttctcccgct aactgatacc cggctaacag tctggcgttt tctttttcag caacgggaaa
     1261 gcaacaacca ccacacccgc caccagcaca ccgtcagccg gcactgacat tatccggctg
     1321 ctgcaatgcc attaacaaaa acagtaagca atcacttttt accgtaacag gtgataatcc
     1381 agatatgtat ctaccccaga tgagtaatcc gaagttcatc cataccacag gtcctggcta
     1441 ttctgttgta ctcctgaaca agagcaaata attctgaatt agcaaccatg aactcatcgc
     1501 aaaccctctg tatagcatca ctattcagaa taataacgtc tcttcccgaa agacgatcag
     1561 gagtacagaa caaaactgtc aaacggctga aggcctttgc tcgtgctgca ttgactatat
     1621 caatacgctg cctaaggatg aaacaccccg acgcctcatc aatattcact ctacccacac
     1681 catatgaatg ataaatactt aatgctgaaa aaaccattag accgtataac aaatactcaa
     1741 tcaatactta acagaacttt tatttttgac aaatatataa tattttcaac aatatcctga
     1801 gccaggtata tttcagtata aggctctgcc ggaaggaatc tggaagaatg aatatggcgc
     1861 gctgtactgg attcgaacca gtgaccgatt gcttagaagg caattgctct gtccggctga
     1921 gctaacaacg ctgaataccg ataatggacc gccatcgggg acccgccccc cgcaccaaca
     1981 accctgttat cgtgtcgtct gctcttcctg ataagctaat ggcggtttgt gatggtggcc
     2041 cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct
     2101 aaagggccgg gagcagaata ataacggtcc gtaattaatt tcgcaataaa aaaacccgct
     2161 cagcggcggg ttgtagaaac tcttctaacg ccaggcataa aacgcccatc atcatgacga
     2221 atttaccaca gattccggaa aaatcaacct tgttacctag ttaccttttt taactgccgc
     2281 tcagcccatg cttcttcaat atcaaaccgg gtcaccagcg catcataaaa tttcttaact
     2341 gttttttccc atgacgcgcg tgttatctgg tttgtcacct cgcatatagc attaaatgcc
     2401 tccgttgatg gtagtctttc atagccacga ccaccacaac gctggcagtc tctgataaca
     2461 ggcataccac gttttaccga ctcttcacga tgaatggcga caccacgccc acggcaatcc
     2521 ttacaggcgg tggaaacctc accctttccg ccacactccg gacaggcaac ttttaccacc
     2581 tccctgactt ttttccattc ttcccagtaa gacggataca cacctttcgt acactttgcc
     2641 cataccggcg gcttaccatc cggatactgg atcttgtttg taaaaactat gctttcaata
     2701 aatttttccc catagcaaca agggcactgc tttttactcg ctgcgctgcg ggcataatcc
     2761 tcaaaagcgt acgaagccat aatgcgcatc actaccggtt ttatttctgc cggaagtttt
     2821 ctcaacgccg ccacacgatc gcaccgactg agtgcataat ctgccagtaa ttctgttgcc
     2881 cgcgccctgt cattcatact gatgcccatt ttccccagga acgcagaaaa acccatctca
     2941 gcccgattct gtgtcatgcc ctgcgcggcc atcacatcag tgatactcag cgcatctttt
     3001 gacgttgagg ccgatgcatc ggtcaggcca ggggattttg gggagtagta tttcggtaaa
     3061 tcttccagtt tcattttttg acctgctctt aatgcattat ggggtaaatc ttcaccccca
     3121 gacgtccacc agatactggc tgaccacgaa cgatattgat ttcatcaaac tgctcatcgt
     3181 ccattaacac tcccgcatgc gtcagcgcat ccagcggtgc tttcaggata ttgtccaggt
     3241 cgcgacgacg cttatccggt ggctctgcaa tcacctttat cgccagcctt ccggacaggc
     3301 ttaatttcag ccgctgctgg cgaacaataa gcgccacagc ccggcgataa cgctttccct
     3361 cctccgagat aaaatatgtg ctgccacggc gtcgccagta agtgttcacc gtcggcgggt
     3421 aaggtaaaac caaatctatg agcatcagtc acctctttta cccaagcacg ccagttgcaa
     3481 aggcgtgatc aagaaaacga aaaattaaat caacctgaga accatgcttt tcttcgaacg
     3541 ccagaggatc cgcatgaagc tcgttgtgat gctcccgaca cagcggtagc gtgaaaatat
     3601 cgtgagattt ggtccccatt ccgccctgac catgaccaat caggtgatgg ggatcgtcgg
     3661 ctggcttacc acaacacgca cacggctgtg tcttcaccca gcgtttgtat ttctcgttaa
     3721 cccagcggcg acgtttaggt cgtttcatga aagattccgg agactcagga tcaacggcaa
     3781 tgctgaccac cgtcttttcc tgttgtgggt tctgttgctg gtgggcgtga ggcagcggcg
     3841 caagattttt tgtgcgctgt ttcagtatgc tggtggcggt ctgctctccc gg
//
LOCUS       DAJVPP010000199         3803 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVPP010000199 DAJVPP010000000
VERSION     DAJVPP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.203"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..124
                     /locus_tag="OQM34_003991"
     CDS             <1..124
                     /locus_tag="OQM34_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1454205.1"
                     /translation="DTVGKNTAKIQDYIKHQLEEDKMGEQLSIPCPGSPFTDRK"
     gene            complement(242..1186)
                     /gene="hypT"
                     /locus_tag="OQM34_003992"
                     /gene_synonym="qseD"
     CDS             complement(242..1186)
                     /gene="hypT"
                     /locus_tag="OQM34_003992"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1454206.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1531..2250
                     /locus_tag="OQM34_003993"
     CDS             1531..2250
                     /locus_tag="OQM34_003993"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1454207.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2316..3614
                     /locus_tag="OQM34_003994"
     CDS             2316..3614
                     /locus_tag="OQM34_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCS1454208.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT          946 a          840 c          927 g         1090 t
ORIGIN      
        1 cgatacagtg ggtaagaaca cggcgaagat acaggattac ataaagcacc agcttgaaga
       61 ggataaaatg ggagagcagt tatcgattcc ctgtccgggc agcccgttta cggaccgtaa
      121 gtaacgaagt tggatgcaaa tgtcagatcg tgtgcgcctg ttagggcgcg gctggtaaga
      181 gagccttata ggcgcatttg aaaaacctcc ggctatgccg gaggatattt attgcacgtc
      241 gtcaattgcg gcgtgctggc tcttccattg atatctgctg gattaaatcg tctggcatat
      301 gttcctgtaa aatacgccag aggttctcgg cggttctatt cagtcgggtg ttcattcggt
      361 aaatataagc ctgaatgggg actgtcaatt tagcagcatc aaggcatatc agacgtttgg
      421 tctgtagttc atcgacgatc gaccagatcg gtaaccaggc aatgccgtat ccgttgagcg
      481 ccatattctt taataactcg ctcatcgaag agataaatat cgtgcgcccg gtaatacctc
      541 caacctctgc cagatgacga ttgaccaacc tgcccatata agatgtggcg gtatagttga
      601 gcaaaggaac ctggggctga gtgatatcga atacagggtg cccctgtgcg tcggcggcac
      661 ataccgggta caactcagat tcaaacagtt tcaggcagca aaaaggcgat tgcataagat
      721 cttcatcgcg aaacgaaata ataaagtcac tcttgccttc gcgtaacgta tttacggcct
      781 ggacaacatc aatggcttca acatgataga caaattctcc gccataggcg gtaagtgaat
      841 gcaccagttt gggtaatacg ctcagggata gtgagtgcgc ggctgcaatt ttaatgttgg
      901 gaacgccgag taaactctgg ccgtttaact cccccaaatt gcattcaagc tgctgtaata
      961 agctgcgagt ctgggaatga aagagcttgc cctcttcggt taattgtaaa ggggtggtcg
     1021 tgcgatcgaa taaaacaacg ccgatcgctg cctccagcgc cttaattcgt cggctgaatg
     1081 cgggttgcga aagattacgc tcttttgccg cctgagaaaa atggcgacag gcttccagtg
     1141 tcaggaaatc gtataaccat tttgtctcga tattcttcat taccatggca cctgtatttt
     1201 caatccaaag acataataaa acatcccggc aataaaaata atgttctcaa ttagcacaac
     1261 ctggccttta tcattcgttt tgtgcataac cccttcattt ccggtattga cgttgatatc
     1321 acatttcgcc aaaaaaacga aatttttccg tcagataacc agaatacatc atcactatgg
     1381 tgaataagcg gaatgttcca tagctgcatc atgcgtgtct tgcatcgcct tgtacaacct
     1441 tgcatttctc atcgcccatt tcgactgtta aaaaagagag cagcagttat ttaccgcgta
     1501 ttgtcattaa cgttttgaga ggcaggtatt gtgaaaggtt taattggtgt actcggtgga
     1561 atggggccgg cagctaccgt cgacctgttt aataaatttg taacttttac cgctgcacaa
     1621 tgtgatcagg aacatattcc tttaattatt tcttcgatcc cggatattcc tgaccgtacc
     1681 gatgcattaa tgcatcatgg gcgttcacca ttacccgtta tgcgtgatta tatgcataag
     1741 ctggaagatg ccggggcaga atgtattgtt attccctgta ataccgcgca ttactggttc
     1801 aaagaattaa aagatgcttg tcatattgat atattaagta tcgtagaaac tacgatcaat
     1861 gaagttcgcg cctgtggtaa aacccgaatt ggcttgttag ccaccaatgc cacgctatat
     1921 atgggtttat accaaaaagg aattgagtcg ctgggattta cctgtgtcag ccccgatgcc
     1981 gacggtcaga aaaaagtgat ggaaagtatt tattccttga aagctggcaa tatagcgcac
     2041 gcacaaaagt tgatgaatga gcaggctgaa atattatttt cccgcggtgc agaaatcctt
     2101 gttcttggtt gtacagaaat tccggttatt ctggcgcaag ccgttaaaga gcagccttcc
     2161 cgctatattg actcaacggc gtcactcgtt cgtgccggaa taaaatggta cgaaaatcgt
     2221 gtcggtaaac attatctttt aacccaataa tcatttaaat cgcagccaga aagttattcg
     2281 cttttaactg aattatattt ataacggaga acattatggt ttggctggaa attatcgtag
     2341 tacttggtgc aatatttttt ggtattcgcc aggggggaat cggtattggt ttatgtggcg
     2401 ggcttgggct tgccattctg actctgggat ttggtctgcc tatggggtcg ccgccagtcg
     2461 acgtgatcct gattattatg accgttgttg tggcggcctc agccttacag gccgcaggcg
     2521 gaatggatta tctggtacgc gtcgctggta attttatgcg gcgtaacccg aaatatatca
     2581 acataatcgc gccgattatc acctggtcta tgaccattat ggccggtact gggtttattg
     2641 ttttttccac gctgccggtg attgctgaag tggcgaaaga gtccggtatc cgtccttccc
     2701 gcacgcttgc cggttcggtt gtcgcttcgc aggttgcgat ttccggttcg ccgatcagcg
     2761 ccgcaatggc ggctatgctc actattatgg aggtaaacgg tgtcagtttt attcaagtga
     2821 tgtcggtatg tcttcccacc tcttttgctg ccgcgatggt cgccgctttt attgcttccc
     2881 gacagggatg tgagttgcag gatgacgagg tctaccttga gcgcctgcaa aaaggactgg
     2941 tgcaaaaata cgagaataat agcagcatca agccaggtgc gacgctctcc gtcggattgt
     3001 tcctgctggc gacggtcgct attgttattc ttgctgcctt cccccaactg cgtccgggtt
     3061 ttgatatcgg taaaccgatg gaaacacgcg atatcattat tatctgtatg ctctctgccg
     3121 cctgcctgat ggtggtgctg tgtaagacgg cgaccgatgc cattattctc acctccacat
     3181 tccgtgcggg gatgagttcg ctggcggtaa ttctcggcat tgtaacctta ggcaccactt
     3241 ttattgatgc gcatttaact gagattaaag atattgctgg tgatattttg caggcgtatc
     3301 cgatgttgtt ggcgctggtt ctttttggca cttgcgcgct gctctactcg caaggtgcga
     3361 ccacgccgtt aattatcccg cttgccgtcg cgcttgacgt gcccacctgg gcaattctgg
     3421 cgtcctatgt ggccgtcacc ggagtatttg tactgccaac gtatccaaca tcactggctg
     3481 ccatggaatt tgatactacc ggcacaactc gtgtaggcaa atatgtgctg aatcatccat
     3541 ttatgctgcc tgggttgggg ggaattattg ctggagtggc attaggcttt gtgattgcgc
     3601 caatgattat ttagcactat caaacatcag tatcgatggt ttatacacaa taaagacaga
     3661 tatggcggaa tatagtctgt tgtaagcgcc aacaaagaat aggggaattc cgccatgtct
     3721 gaacatcttc acaatatggt attaagtgtt gtcgtttaat gatttatttc ttggttatgt
     3781 tgtgtattag ctgcatctat cgc
//
LOCUS       DAJVPP010000200         3795 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVPP010000200 DAJVPP010000000
VERSION     DAJVPP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.204"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..311
                     /locus_tag="OQM34_003995"
     CDS             <1..311
                     /locus_tag="OQM34_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1454209.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OQM34_003996"
     CDS             468..1217
                     /locus_tag="OQM34_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1454210.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OQM34_003997"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OQM34_003997"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCS1454211.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OQM34_003998"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OQM34_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1454212.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3795
                     /gene="yieH"
                     /locus_tag="OQM34_003999"
     CDS             3361..>3795
                     /gene="yieH"
                     /locus_tag="OQM34_003999"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS1454213.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALM"
BASE COUNT         1042 a          977 c          939 g          837 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agtctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctacg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatg
//
LOCUS       DAJVPP010000201         3732 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVPP010000201 DAJVPP010000000
VERSION     DAJVPP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.205"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            178..1158
                     /gene="rluD"
                     /locus_tag="OQM34_004000"
     CDS             178..1158
                     /gene="rluD"
                     /locus_tag="OQM34_004000"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1454214.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1155..1886
                     /gene="pgeF"
                     /locus_tag="OQM34_004001"
     CDS             1155..1886
                     /gene="pgeF"
                     /locus_tag="OQM34_004001"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1454215.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     AENETFFSYRRDKTTGRMASFIWLI"
     gene            2016..>3732
                     /locus_tag="OQM34_004002"
     CDS             2016..>3732
                     /locus_tag="OQM34_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1454216.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIG"
BASE COUNT          949 a          909 c         1018 g          856 t
ORIGIN      
        1 ggcttagtgt ggcggctgcc accagatatt tcatgcgcgt catgacgttt tgactttcct
       61 caaaatgtaa tacgggagat tctctgttcc tgcacccggt taagaccagc tacaatagca
      121 cactatatta aacggcaaag ccgtaaaacc ccaacgataa acgaagaagc agtatatatg
      181 gcacaacgag tacagctcac tgcaacggtg tccgaaaacc aactcggtca acgcttagat
      241 caggctttgg ccgaaatgtt cccggattat tcacgttcgc gaataaaaga atggatcctc
      301 gaccagcgcg tgctggtaaa cggcaaagtt tgtgataagc cgaaagaaaa agtattgggt
      361 ggcgagcagg ttgccatcaa cgctgagatt gaagaagaag cgcgttttga accgcaggat
      421 atccctctgg atatcgtcta tgaagatgaa gatatcatcg tcattaataa accgcgcgac
      481 ctagtggtac atcctggcgc gggtaacccg gatggcacgg tactgaatgc gttgcttcat
      541 tactatccgc ccattgccga tgtaccacgt gcgggcatcg tccatcgtct ggataaagac
      601 accactggcc tgatggttgt ggcaaaaacc gttccggctc agacgcgttt agtcgaatct
      661 ttgcaacggc gtgaaattac tcgtgagtat gaagcggtgg cgattggtca tatgaccgct
      721 ggcggtacag tagatgaacc catcagccgc cacccgacca aacgtactca catggcggtg
      781 catccgatgg gcaaaccggc ggtgactcac tatcgcatca tggaacactt ccgtgtgcac
      841 acgcgtctgc ggttgcgtct ggaaactgga cgtacgcacc agatccgcgt gcatatggcc
      901 catatcactc atccgctggt gggcgatccg gtttatggtg gccgtccgcg tccgccaaaa
      961 ggtgcttcgg aagcatttat ctccacgctg cgtaagtttg accgccaggc gcttcatgca
     1021 accatgctgc gtctttatca cccgatctcc ggcatcgaaa tggaatggca tgcgcctatt
     1081 ccacaagata tggtggagct gattgaggtg atgcgcgccg atttcgaaga gcataaggat
     1141 gaagtggact ggttatgagt aagctgattg tcccgcagtg gccgctgcca aaaggtgttg
     1201 cggcctgtag ctccactcgt atcggcggcg tgagcttgcc cccgtatgac tcactcaacc
     1261 tcggtgccca ttgtggcgat aacccggatc acgttgagga gaatcgcaag cgactttttg
     1321 ctgcgggcaa tttgccttct aaaccggtct ggcttgagca ggtacacggc aaagatgtgc
     1381 ttaagcttac tggcgaacct tatgcctcaa aacgagcaga tgcctcttat agcaatacgc
     1441 ctggcacggt ttgcgcagtg atgactgccg actgcctccc tgtgctgttt tgcaatcgag
     1501 cgggaacgga agtcgccgcc gttcatgctg gctggcgtgg actgtgcgca ggcgtgctgg
     1561 aagagacggt ttcctgtttt gctgataaac ctgaaaacat tctcgcctgg ttagggccgg
     1621 caattggtcc acgcgcgttc gaagtggggg cggaggttcg cgaggcgttt atggcagtag
     1681 acgctaaagc aagtgcagct ttcattcagc atggtgataa gtatctggcg gatatttatc
     1741 agcttgcccg gcagcgtctg gcgaacgtgg gtgttgagca aattttcggc ggcgaccgtt
     1801 gtacatatgc ggaaaatgag actttcttct cttatcgtcg cgacaagacc accggtcgta
     1861 tggcaagttt catttggctg atataaccta aagaatcaag acgatccggt acgcgtgatt
     1921 ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc tcatttaatc
     1981 tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt cttactaata
     2041 aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac aaccaattca
     2101 tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg gttagtcctt
     2161 tattaacatc cgctggcata aatgctggcc agttgcgcac agatatcaat caggcattaa
     2221 atcgtttacc gcaggttgaa ggtactggtg gtgatgtcca gccatcacag gatctggtgc
     2281 gcgttcttaa tctttgcgac aagctggcgc aaaaacgtgg tgataacttt atctcgtcag
     2341 aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc ctgaaagcaa
     2401 caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga ggtgaaagcg
     2461 tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc atcgacctta
     2521 ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa gaaattcgcc
     2581 gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt ggtgaacccg
     2641 gcgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac ggcgaagtgc
     2701 cggaaggatt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg gtggctgggg
     2761 cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat cttgccaaac
     2821 aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggc gcgggtaaag
     2881 ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt ggtgaattgc
     2941 actgcgtagg tgccacgacg cttgacgaat atcgccagta catcgaaaaa gatgctgcgc
     3001 tggaacgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagat accattgcga
     3061 ttctgcgtgg cctgaaagaa cgttacgaat tgcaccacca tgtgcaaatt actgacccgg
     3121 caattgttgc agcagcgacg ttgtctcatc gctacattgc tgaccgtcag ctgccggata
     3181 aagccatcga cctgatcgat gaagcagcat ccagcattcg tatgcagatt gactcaaaac
     3241 cagaagaact cgaccgactc gatcgtcgta tcatccagct caaactggaa caacaggcgt
     3301 taatgaaaga gtctgatgaa gccagtaaaa aacgcctgga tatgctcaac gaagaactga
     3361 gcgacaaaga acgtcagtac tccgagttag aagaagagtg gaaagcagag aaggcatcgc
     3421 tttctggtac gcagaccatt aaagcggaac tggaacaggc gaaaatcgct attgaacagg
     3481 ctcgccgtgt gggggacctg gcgcggatgt ctgaactgca atacggcaaa atcccggaac
     3541 tggaaaagca actggaagcc gcaacgcagc tcgaaggcaa aactatgcgt ctgttgcgta
     3601 ataaagtgac cgatgccgaa attgctgaag tgctggcgcg ttggacgggg attccggttt
     3661 ctcgcatgat ggaaagcgag cgcgaaaaac tgctgcgtat ggagcaagaa ctgcaccatc
     3721 gcgtcattgg tc
//
LOCUS       DAJVPP010000202         3686 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVPP010000202 DAJVPP010000000
VERSION     DAJVPP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.206"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..221
                     /locus_tag="OQM34_004003"
     CDS             <1..221
                     /locus_tag="OQM34_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1454217.1"
                     /translation="QRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPV
                     LQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            267..476
                     /gene="hha"
                     /locus_tag="OQM34_004004"
     CDS             267..476
                     /gene="hha"
                     /locus_tag="OQM34_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1454218.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            515..1105
                     /locus_tag="OQM34_004005"
     CDS             515..1105
                     /locus_tag="OQM34_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1454219.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1345..1605
                     /gene="repA"
                     /locus_tag="OQM34_004006"
     CDS             1345..1605
                     /gene="repA"
                     /locus_tag="OQM34_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCS1454220.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1829..1903
                     /gene="tap"
                     /locus_tag="OQM34_004007"
     CDS             1829..1903
                     /gene="tap"
                     /locus_tag="OQM34_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCS1454221.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1896..2753
                     /gene="repA"
                     /locus_tag="OQM34_004008"
     CDS             1896..2753
                     /gene="repA"
                     /locus_tag="OQM34_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCS1454222.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3453..3644
                     /locus_tag="OQM34_004009"
     CDS             3453..3644
                     /locus_tag="OQM34_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454223.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          994 a          900 c          931 g          861 t
ORIGIN      
        1 ttcagcgaga tcgttatggt cgtattctcg ggcaggttta tgcaccagac ggaatgaata
       61 ttaatcagtt tatggtccgg gccggggctg cctgggtata tgagcagtac aacacagatc
      121 ccgtgctacc tgtccttcag aacgaagcca gacaacaaaa acgtgggctc tggtcagatg
      181 ctgatccggt tcccccctgg atatggaggc accgtaaata aagcgatggc aatcctatca
      241 ttcatctgtt ctcagcggga ataaaaatgg caaaaacaaa acaggaatgg ctgtatcagt
      301 tacggcgatg ttcatcagtc aatacgctgg aaaaaattat tcataaaaac agagacagtt
      361 tatcaaacag tgaacgtgaa tcatttaatt cagcagcaga ccaccggcta gcggaattaa
      421 tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta aggtaataat
      481 tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt ttattctcat
      541 tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa atcagcattc
      601 tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag agcgaatact
      661 gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg aagtcagttt
      721 tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc cgcaggtacg
      781 ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc agtggtggca
      841 gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc tgcgttcgtt
      901 cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg aacgcagacg
      961 ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc tcagaagcca
     1021 cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag gggctgatca
     1081 gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca accccggaaa
     1141 cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc atactatttc
     1201 acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca acacagatct
     1261 tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc
     1321 ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac ttcctcattg
     1381 agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa acaaatggcc
     1441 gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa aaatcctctg
     1501 aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca gttcgttgag
     1561 aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact ttactttgtg
     1621 gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac gccataaggt
     1681 ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa ccccgataat
     1741 cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg tatagccaac
     1801 aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact tctttctgtg
     1861 ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa caaacttatt
     1921 accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc cggacgcttc
     1981 cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt gatttcgcca
     2041 ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg gtactacgtc
     2101 gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca ctggccaacc
     2161 gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg gagtctgctg
     2221 ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca gagctgggac
     2281 tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg accgatatca
     2341 cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg gccgccgcgc
     2401 gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg gataccctgg
     2461 gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc cgcagttacc
     2521 agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat gcggacaggc
     2581 agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc tcggaagggc
     2641 gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg aaggagcgca
     2701 tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc tgaaagtgac
     2761 ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc cgccagtgca
     2821 caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttctgg tgcatccggt
     2881 cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt atccacatta
     2941 aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg ccagacgtca
     3001 accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta aactacttaa
     3061 ttacattcat ttaaaaaaaa aacctgttca ctgcctgtcc tgtggacaga cagatatgca
     3121 cctcccaccg caagcggcgg gccccaaccg aagccggttt agttacaaca tgcaaacata
     3181 aacccctaaa aaacccaagt ccaacgcaga actgaaacca caacgccccc ctcataactg
     3241 aaaagcgccg ccgcccccgc ccaaagggcg ggaacagagt cgcttttaat tatgaatgtt
     3301 gtaactacat catcatcgct gtcagtcttc tggctggacg tatcgagtac acgctcgtaa
     3361 gcggccctca cggcccgcta acgcggagat acgccccgac ttcgggtaaa accctcgtcg
     3421 ggaccactcc gaccgcgcac agaagctcta tcatggctga aaacgggtat ggcttggcag
     3481 ggctggggat gggtaaggtg aaatctgtca atcagtaccg gctgacgccg ggcttcggcg
     3541 gttttactcc ggtatcacat gtaaccacag cgtgccgcct tccatgccgc tggcgcggca
     3601 tcagaataat acaagcagca tttaatgcat ttgcaaaagt gtgagtaata ttaagtggtc
     3661 gtttcaggga tatcgttaag acttga
//
LOCUS       DAJVPP010000203         3617 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVPP010000203 DAJVPP010000000
VERSION     DAJVPP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.207"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..2043
                     /gene="ydeP"
                     /locus_tag="OQM34_004010"
     CDS             <1..2043
                     /gene="ydeP"
                     /locus_tag="OQM34_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1454224.1"
                     /translation="KQVNASFFAENTVQSLLTWGDHELEAAGRLTQPLKYDAVSDCYK
                     PLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAAFLYQLFAREYGSNNFPDCSNM
                     CHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHNPGTNHPRMLTSLRALVKRGAK
                     MIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYYNVRIGGDMALLKGMMRLLIER
                     DDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEWKDIERISGLSQTQIAELADAY
                     AAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNIGKPGAGICPLRGHSNVQGDRT
                     VGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQAICTGQARALICMGGNFALAMP
                     DREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILPVLGRSEIDMQKSGAQAVTVED
                     SMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQSVVAWEYLVEDYDRIRNDIEAV
                     LPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANFITSKGLLEDPSSAFNSKLVMA
                     TVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAKICRVKNGERVNLIALTPDGKR
                     SSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHDPLSGIPGYKSIPVELEPSN"
     gene            2291..2494
                     /gene="safA"
                     /locus_tag="OQM34_004011"
     CDS             2291..2494
                     /gene="safA"
                     /locus_tag="OQM34_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1454225.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            2562..3323
                     /gene="ydeO"
                     /locus_tag="OQM34_004012"
     CDS             2562..3323
                     /gene="ydeO"
                     /locus_tag="OQM34_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1454226.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1012 a          794 c          818 g          993 t
ORIGIN      
        1 aagcaggtaa atgcctcttt ctttgctgag aatacggttc aatcattact tacctgggga
       61 gatcacgagc ttgaggctgc ggggcgtctc actcagcctt tgaaatatga tgccgtcagc
      121 gactgttaca agccattaag ctggcaacaa gctttcgacg aaattggcgc acgccttcaa
      181 agctatagtg atcccaatca ggttgaattc tatacttcgg gccgcacttc caatgaagct
      241 gcctttcttt atcagctttt tgcccgtgaa tacgggagca ataactttcc cgactgctcc
      301 aacatgtgcc atgaaccgac aagcgtgggt ttggcagcga gtatcggtgt aggtaaaggg
      361 accgtgttgc tggaagactt tgagaagtgc gatttagtca tttgcattgg gcataaccct
      421 ggtacaaacc accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg gggagcgaaa
      481 atgatcgcca tcaatcctct acaggaacgt ggcctggagc gatttaccgc accgcaaaac
      541 ccgtttgaaa tgctgacgaa ctctgagact cagttggcca gtgcctacta caacgtgcgc
      601 attggtggcg atatggcgtt gctcaagggg atgatgcgcc tgttaattga gcgcgatgat
      661 gctgcaagtg ccgcaggtcg gccctcattg cttgatgacg aatttattca aacgcatacc
      721 gtcggctttg acgagctacg ccgtgacgtt ctcaattccg agtggaaaga tatcgaacgt
      781 atttctggac taagtcagac acaaatcgcc gaactggctg acgcatatgc cgctgccgaa
      841 cgcaccatta tctgttacgg aatggggatc actcagcacg aacatggtac ccagaacgtg
      901 cagcaactgg tcaatttgct gttgatgaaa ggtaacattg gcaagcctgg tgcgggtatc
      961 tgcccactac gtggtcactc taatgtacag ggcgaccgaa ccgtcggtat caccgagaaa
     1021 ccgtctgcag agtttctggc tcgcctgggt gagcgctatg gcttcccccc acctcatgca
     1081 cctggacatg ctgcaattgc cagcatgcaa gcaatatgta cggggcaggc tcgagcattg
     1141 atctgcatgg ggggcaactt tgcactggca atgccagatc gggaagcgag cgctgtaccg
     1201 ttaacgcaat tagatttggc ggtacacgta gccactaagc ttaaccgctc tcatctgctg
     1261 accgcacggc atagctatat tctgccggtt ctggggcgta gcgagattga tatgcaaaaa
     1321 agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga tgattcatgc ctcgcgtggc
     1381 gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg cagtggtcgc gggaatcgcg
     1441 caggcagcac taccccagag cgtggtagcc tgggagtatc tggtggaaga ttatgatcgc
     1501 attcgcaatg acattgaagc tgtgctgcca gagttcgccg actataacca gcgcatccgt
     1561 catcccggtg gttttcacct gataaatgca gctgctgaaa ggcgctggat gacgccgtca
     1621 ggtaaggcta atttcattac cagcaaaggg ctgttagaag atccctcttc agcgtttaac
     1681 agtaagctgg tcatggcgac agtacgcagc cacgatcagt acaacacgac gatttatggt
     1741 atggatgatc gctatcgagg ggtattcggt caacgagatg tggtctttat gagtgctaaa
     1801 caagctaaaa tttgccgtgt aaaaaacggc gaaagagtta atcttattgc gcttacgcca
     1861 gacggtaagc gcagctcacg ccgcatggat agattaaaag tggtcattta ccctatggct
     1921 gaccgctcac tggtgaccta ttttccagaa tcgaatcaca tgctaacact tgataaccac
     1981 gatccattaa gtggcattcc tggctataaa agtattccgg ttgaattaga accatcaaat
     2041 taatgcctct tctcatttct tctgctgtca tccgcacagc agaagaattc ctcattgact
     2101 attattgcgc aatttgctca catggattaa attaaactac atactataag atataaactt
     2161 ctgcctacag ctgtaagaaa ctccgctcag tactgaagca ccagtcctat ttcttctttt
     2221 ctccagcctg ttatattaag catactgatt aacgactttt aacgttatcc gctaaataaa
     2281 catatttgaa atgcatgcga ccacagtgaa aaacaaaatc acgcaaagag ataactataa
     2341 agaaatcatg tctgcaattg tggttgtctt attactgaca cttacgttga tagccatttt
     2401 ttcggcaatt gatcagctga gtatttcaga aatgggtcgc attgcaagag atcttacaca
     2461 tttcattatc aatagtttgc aagactggaa ataaacagca aattataaat gtgaaatgtt
     2521 taaaaggtat cgataaaact ttattgtttt aaggagataa aatgtcgctc gtttgttctg
     2581 ttatatttat tcatcatgct ttcaacgcta acattttaga taaagattac gccttctccg
     2641 acggcgagat cctgatggta gataacgctg ttcgtacaca ttttgaacct tatgagcggc
     2701 attttaaaga aatcggattt aatgaaaata ccattaaaaa atatctacaa tgcactaaca
     2761 tccagacagt gacggtgcct gttcctgcga agtttttacg tgcttcaaat gtaccgactg
     2821 gattgcttaa tgaaatgatt gcttatctca actcggaaga acgcaatcat cataattttt
     2881 cagaactttt gcttttttct tgcctgtcta tttttgccgc atgcaaaggt ttcattacac
     2941 tattaactaa cggtgtgcta tccgtttccg ggaaagtgag aaatattgtc aacatgaagc
     3001 tggcgcaccc atggaagctg aaagatattt gtgactgcct gtacatcagt gaaagcctgt
     3061 tgaagaaaaa actaaagcaa gagcaaacga cattctcaca gattctatta gatgccagaa
     3121 tgcagcacgc aaaaaatttg atacgcgtag aaggttcagt caataaaatt gccgaacaat
     3181 gtggttatgc cagtacatct tattttattt atgcgttccg caaacatttc ggcaacagtc
     3241 cgaagagagt ttctaaggag taccgttgtc aaaggcacac gggtatgaat acgggcaaca
     3301 cgatgagtgc tttagctatt tgattatttg ctaacgagta gtcaaccacg cacgctacgt
     3361 aagaattata tggggcagcc attccctgcc ccgcgttgtt tttaggcgat atatttattg
     3421 aaataaataa gtgacatcca tcacatattt atgcacttgc ataacctgtt gcatgattat
     3481 ttatgatctc aattcggcat tttgtcagta aaatgcaata atttattaaa tatcaataaa
     3541 ttagttgttt atcggcgaga aattacttaa tagaacagaa agtaatgtca acgctttatg
     3601 gactgttatt tcccttt
//
LOCUS       DAJVPP010000204         3576 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVPP010000204 DAJVPP010000000
VERSION     DAJVPP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.208"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            203..1483
                     /gene="kbaZ"
                     /locus_tag="OQM34_004013"
     CDS             203..1483
                     /gene="kbaZ"
                     /locus_tag="OQM34_004013"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCS1454227.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            1506..1979
                     /gene="agaV"
                     /locus_tag="OQM34_004014"
     CDS             1506..1979
                     /gene="agaV"
                     /locus_tag="OQM34_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCS1454228.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            1990..2769
                     /locus_tag="OQM34_004015"
     CDS             1990..2769
                     /locus_tag="OQM34_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCS1454229.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            2759..3568
                     /locus_tag="OQM34_004016"
     CDS             2759..3568
                     /locus_tag="OQM34_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCS1454230.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
BASE COUNT          823 a          949 c          949 g          855 t
ORIGIN      
        1 aacgcctttc tattaagcaa atgcaagccc accttgccca ttggcgcaag ctactctcgt
       61 ttcagtgact ttcattacgt ttcttttgtg aatcagatca gaaaactatt atctttcgtt
      121 ttatttttat ctcaccatga cgcagtatca attgaaacaa aacgaaagat caatatcgca
      181 gtaatctgaa ctggagagga aagtgaaaca tctgacagaa atggtgagac agcacaaagc
      241 gggcaaaaca aatgcaattt atgccgtttg ttccgcacat ccgctggtgc tggaggctgc
      301 aatccgctac gccagtgcaa accaaacgcc gttactgatt gaagcaacct ccaatcaggt
      361 agaccagttc ggcggttata ccggaatgac gcccgccgat tttcgcggct ttgtttgtca
      421 gctcgccgac tcgttgaatt tcccgcagga tgcgctgatt ctgggtggtg accatctggg
      481 gccaaaccgc tggcaaaacc tgccagccgc tcaggcaatg gccaatgccg atgatttgat
      541 taaaagctac gttgcggcag gattcaaaaa aatccacctt gattgcagca tgtcctgtca
      601 ggacgatcca attcccttaa ctgatgacat cgtggctgaa cgcgccgccc gtctggcgaa
      661 agtggcggaa gaaacctgtc gcgaacactt tggcgaagcc gatctggagt atgtcattgg
      721 taccgaagtg ccggtacctg gcggcgcgca tgaaacctta agcgagctgg cggtcaccac
      781 gccggatgcc gcccgcgcca cgctggaagc ccatcgtcac gcctttgaaa agcaaggttt
      841 gaatgccatc tggccacgca ttattgccct ggtggttcaa cccggcgtcg aattcgatca
      901 caccaacgtt attgattatc agcccgccaa agcgagcgcc ttaagccaga tggtcgaaaa
      961 ctacgaaacg ctgattttcg aagcgcactc taccgattat caaacgccgc aatctctgcg
     1021 ccagctggtg attgaccact ttgccattct gaaagttggc ccggcgctga ccttcgccct
     1081 gcgtgaagcc cttttttctc tggcggcgat tgaagaagaa ctggtaccag cgaaagcctg
     1141 ttccggtctg cgtcaggtgc tggaaaacgt gatgctcgac cgcccggaat actggcaaag
     1201 ccactaccac ggtgacggca acgcgcgtcg tctggcgcgt ggttacagct actcagatcg
     1261 cgtgcgctat tactggccgg acagccagat tgatgacgct ttcgctcatc tggttcgtaa
     1321 tctggcggat tcaccaattc cgctaccgct catcagccag tatctgccgc tgcagtacgt
     1381 gaaagttcgc tccggcgagc tgcaaccgac gccacgggaa ctcattataa accatattca
     1441 ggacatcctg gcgcagtacc acacagcctg tgaaggccaa taagcaaaac aaagaggaac
     1501 atgctatgcc aaatattgtt ttaagccgga ttgatgaacg cttgattcac ggtcaggtcg
     1561 gcgttcagtg ggtcggattt gcgggggcaa atctggtgct ggtagccaac gatgaggttg
     1621 ccgaagatcc ggtacaacaa aacctgatgg aaatggtact ggcagaaggg atcgccgtac
     1681 gtttctggac gctgcaaaaa gttatcgaca acattcatcg cgccgccgat cgacagaaaa
     1741 tcctgctggt ttgtaaaaca cccgccgatt tcctgacgct ggtgaaaggt ggcgttccgg
     1801 tgaatcgcat taacgttggc aatatgcact acgccaatgg caaacaacaa atcgccaaaa
     1861 cggtttctgt ggatgcgggc gatatcgcag catttaacga cctgaaagcc gctggggtgg
     1921 aatgcttcgt tcagggcgtc ccgacagagc ctgctgtgga cctctttaaa ttactttgag
     1981 ggattcatca tggaaatcag cctgttgcag gcatttgcgt tgggcattat cgcctttatc
     2041 gctggcctag atatgtttaa cggcttaacc catatgcacc gcccggtggt cctcggcccg
     2101 ttggtcgggc tggtacttgg cgatctgcat accggaattt taaccggcga tacgctggaa
     2161 ctagtgtgga tggggctggc cccgctggcg ggcgcacagc cgcctaacgt gattatcggt
     2221 actatcgtcg gcacggcgtt tgccattact actggcgtga aacccgatgt cgcggtcggt
     2281 gtcgccgtac ctttcgctgt cgcagtacag atggggatta ccttcctgtt ctcggtgatg
     2341 tctggcgtga tgtctcgctg cgaccggatg gcggagaacg ccgatactcg cggcattgaa
     2401 cgcgtgaact atctggcact gctggcactc ggcactttct attttctctg cgctttcctg
     2461 cctatctact tcggcgcgga acatgcaaaa accatcattg atgtcctgcc gcaacgatta
     2521 atcgacggcc tcggcgtcgc gggcggcatc atgccagcaa tcggttttgc cgtgctgctg
     2581 aaaatcatga tgaaaaacgt ctacataccc tacttcatcc tgggctttgt tgccgcaacc
     2641 tggctcaagt taccggtgct ggctattgct gccgccgcgc tggcgatggc gctgatcgat
     2701 ctgctgcgta aatctcctga accgactcaa cctgcggcac agaaagagga atttgaagat
     2761 ggcatctaat caaaccaccc tgccgaacgt cagcgaaaat gaagaaacac tgctgactgg
     2821 cgtcaatgaa aacgtgtatg aagatcggag cattgccgcg gagctgacga aaaaagatat
     2881 caaccgcgtc gcctggcgtt ccatgctgtt acaagcgtct tttaactacg aacgtatgca
     2941 ggcttctggc tggttgtacg gtctgctgcc tgctctgaaa aagatccaca ctaataaacg
     3001 cgacctggcg cgcgccatga aggggcatat gggtttcttc aatacccatc cgtttctggt
     3061 gacgtttgtt atcggcatta tccttgcgat ggagcgttct aagcaggacg ttaacagtat
     3121 tcagagcacc aaaattgccg tcggtgcgcc gcttggcggg attggcgatg cgatgttctg
     3181 gctaacgcta ctgccgattt gtggcgggat aggcgcaagc ctcgcgctac aaggctctat
     3241 tcttggcgca gtcgtcttta ttgtgctgtt caacgtggta cacttggggc tgcgttttgg
     3301 tctggcgcat tatgcttacc gcatgggcgt ggcggcgatt ccccttatta aagcgaatac
     3361 caaaaaagtc ggccatgctg cgtcaatcgt tggaatgacg gtaatcggcg cactggtggc
     3421 aacctatgtt cgtttaagca ccacgctgga aatcacggcg ggcgacgcag tagttaagtt
     3481 acaggctgat gttatcgaca aactgatgcc tgccttctta ccgctggtct acaccctgac
     3541 catgttctgg ctggtacgcc gcggctagag tccgct
//
LOCUS       DAJVPP010000205         3522 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVPP010000205 DAJVPP010000000
VERSION     DAJVPP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.209"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..110)
                     /gene="ydcY"
                     /locus_tag="OQM34_004017"
     CDS             complement(<1..110)
                     /gene="ydcY"
                     /locus_tag="OQM34_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 domain-containing protein"
                     /protein_id="HCS1454231.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAEL"
     gene            complement(196..369)
                     /gene="ortT"
                     /locus_tag="OQM34_004018"
     CDS             complement(196..369)
                     /gene="ortT"
                     /locus_tag="OQM34_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCS1454232.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            564..659
                     /gene="yncL"
                     /locus_tag="OQM34_004019"
     CDS             564..659
                     /gene="yncL"
                     /locus_tag="OQM34_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCS1454233.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(1061..1870)
                     /locus_tag="OQM34_004020"
     CDS             complement(1061..1870)
                     /locus_tag="OQM34_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCS1454234.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSELPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(2080..2763)
                     /locus_tag="OQM34_004021"
     CDS             complement(2080..2763)
                     /locus_tag="OQM34_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCS1454235.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNEEQVVNWANDSQYGLA
                     SSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLSGYGKDMSLYGLEDYT
                     VVRHVMVKH"
     gene            complement(2785..>3522)
                     /locus_tag="OQM34_004022"
     CDS             complement(2785..>3522)
                     /locus_tag="OQM34_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1454236.1"
                     /translation="FLHFPILIIAAYAFNTEDAAFSFPPQGLTLRWFSVAAQRSDILD
                     AVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAISLLLLLPIALPGIVTGLALL
                     TAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTSWSLVEASMDLGANGWQTFR
                     YVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTLPLWLLNQLGRPRDVPVTNV
                     VALLVMLVTTLPILGAWWLTREGDNGQ"
BASE COUNT         1016 a          864 c          675 g          967 t
ORIGIN      
        1 cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc aatgacgctc
       61 tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat actttctcct
      121 tagccacttg tgaaatttat cgattaatcc aaatgatata agtctgcgtg gtgccgtcaa
      181 gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac tcatcggcca
      241 tgtggccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt gagaaagaaa
      301 ccaggtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca tatgttgata
      361 gagagacatg tacgggcgtc cagccatatg aataaaacgc gcgcaaatat gcgccatttt
      421 ttgacgtgca tcaagttttt tcaccagttt gagaaaaaat catcaaacag caacttgccc
      481 ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta tacacatagg
      541 ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta ctgataaatg
      601 tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg tttatttgat
      661 gtagtgattt tgcacgcagc acgtactgtc agagaaatca actctctgac agtacgcaag
      721 atcttctcat actctccaag tttgcaacat tatccttaat atttacttct ttttgctggc
      781 atctacattg aaatgagtag aaaacatccc cctactgtcg tctatacaaa cagcttactg
      841 attcaaacgg aatcttgttt tcacattttt ctatcacgaa tactttatac gcgcgggagt
      901 ctataaatgc tttatacttt caaggaataa cgtaaatata aaaagatggc gtaaagtata
      961 tgcatggcag gatgtttgca acctccttga tgacgctgcc ccattgaaat tgcaaacccc
     1021 gccctggtta tcttgcccat ttatcatgca aaacagataa ttataggcct ctgaatacct
     1081 cagaaagatt gaatttttta tagtccctct cttgaatatt tccatttgat atcaatcgat
     1141 tcatctcttc aacaaaataa tttatatcat cacacttatt attatccgga agttctgagt
     1201 agttataatt ccaccatttt attctcaaca gttcatcaat aacatttgaa tcaaacctaa
     1261 atctgattat ttttgcagga actcctgcaa ctatggcata tgggggcaca tctttggtaa
     1321 caacggaatt ggcagcaata acagcaccat cacctattgc gatgccacct ttcaatacaa
     1381 catcatttcc tatccagaca tcatttccta taatcggatt ttcaggtttg gtaatgaatg
     1441 gtttcaatgt ccagctacgt tcaaattcac tcatagcaaa cttatcaaat tcaccgttat
     1501 aagtcatcat gtgagtagta aaacgattca aaggatgatt tccacccatt atttttacat
     1561 ttgatgcaat actacaaaat cgacctatac gtatattccc tgaaaaatgg caggtcggaa
     1621 atgaaaaagc gccgacactg gagaaatttt tcacaggcat tgttgaatat tcttcagcaa
     1681 taacattatc ttcaattgtt atcttgtcac cttttgagaa aagaaataaa ttcctttctt
     1741 tatacgactt cttcccctta tgatttaaaa agattttatt atccatacaa aactcaaagt
     1801 gttttttcgt ccatctgaag gtaagggttt tatttttatc ttccgtgaat ttgtacaatt
     1861 gaactttcat tgttaatact tctacttata aagtattagt ttaataacga tactctaaag
     1921 ccttatgcta atcaactgtg acaggatgaa aaatcgccat caaacattta gcctaatttt
     1981 tacgccagtt attaatgtaa tcattctaat acttaaccgc cattaaactc agaacctaaa
     2041 acgatagtgg ccgtcctata acagccacta acatctaact taatgtttaa ccatgacgtg
     2101 gcggacgacg gtgtaatcct ccagcccata aagtgacata tccttgccgt aaccagaaag
     2161 tttctgccca ccgtgcggca tttcacttac cagcatgaaa tgggtattga cccaggtaca
     2221 accatattgc agccgtgcgc tgacgcgatg cgccctgccc acatctttcg tccataccga
     2281 agatgcaagt ccgtactggc tgtcattcgc ccagttcacc acctgttctt cgttgtcgaa
     2341 gggcgtaaca ctcactactg gaccaaatac ctctttttgc acgatggcat cgtcctgtaa
     2401 tgcgccagcc agcagcgtcg gcgcacccgc gaaaaagcga aaaacatcga caatcgccgg
     2461 gatttcatca ttgaacgcac tatgcagcgg tttgccacaa ttacgggatt ccagttcggc
     2521 aaaaacctga ccattttctt cgataacatc cgccagtttc agcaggcatt ccgcacgcgc
     2581 tttcggtgtg gtttgccccc attcagcaaa tgctgcatct gccgcacgca cagcagcatc
     2641 gacctgctct gcggatacct cggcaatctc cagtaaaacg tcccccgttg ccggattata
     2701 gacaggctgt ttttcccctt cgccgctaac ccgttctccg ttaatcagta acttatgttg
     2761 catagcttat tcctgtatca gtggttattg accattgtcg ccttcgcggg ttagccacca
     2821 ggcccccagg atcggcaagg ttgttaccaa cataaccagc agtgccacca cgttggttac
     2881 cggtacatca cgcggtcgcc caagctgatt gagcaaccac aacggtaacg ttcgttcatg
     2941 acctgccgta aaggtcgtaa cgatgatttc atcgaacgac aaggcaaacg ccagcattcc
     3001 tcctgccagt aacgccgaac tgagattcgg caacactacg tagcggaagg tttgccagcc
     3061 attggcccca agatccattg acgcctcaac cagactccac gaggtgcggc gaaaacgggc
     3121 gatgacattg ttaaacacca ccactacgca aaaagtcgca tgaccgacca cgatggtgaa
     3181 aaatcccggc tccagattga tggttttaaa ggcggttaat agcgccagac cagtgacaat
     3241 gcccggcagc gcaatgggca gcagcaataa cagcgaaatg gcgtttttgc cgaaaaagtc
     3301 tcgtctccac agcgcggcag ctgccagcgt ccctaacacc agcgcaatta atgtcgccag
     3361 cgccgccact ttaagtgaca gcgtcacggc atcaagaata tcactacgct gtgctgccac
     3421 gctaaaccag cgcagcgtca ggccctgcgg tggaaaacta aacgccgcat cttcagtgtt
     3481 aaacgcatag gcggcgatta tcaggatggg aaaatgtagg aa
//
LOCUS       DAJVPP010000206         3179 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJVPP010000206 DAJVPP010000000
VERSION     DAJVPP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.213"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OQM34_004023"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OQM34_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1454237.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OQM34_004024"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OQM34_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          802 a          660 c          773 g          944 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggta gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJVPP010000207         3179 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVPP010000207 DAJVPP010000000
VERSION     DAJVPP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.214"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            42..1073
                     /gene="ynaI"
                     /locus_tag="OQM34_004025"
     CDS             42..1073
                     /gene="ynaI"
                     /locus_tag="OQM34_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS1454238.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1124..2737)
                     /gene="mppA"
                     /locus_tag="OQM34_004026"
     CDS             complement(1124..2737)
                     /gene="mppA"
                     /locus_tag="OQM34_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1454239.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3074..>3179)
                     /locus_tag="OQM34_004027"
     CDS             complement(3074..>3179)
                     /locus_tag="OQM34_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1454240.1"
                     /translation="TPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          844 a          726 c          704 g          905 t
ORIGIN      
        1 tccggcatga cgacaattat ctggaggagc ctgataaaac aatgatcgct gaactgttta
       61 caaataatgc gcttaatctg gtcattattt tcggtagctg cgcagcattg attctgatga
      121 gcttttggtt tcgccgtgga aatcgtaaaa gaaaaggatt tttattccat gcggtgcaat
      181 ttttaatcta caccataatt atcagtgctg ttggtagcat cagtaattat gttatagaaa
      241 actataaact caaatttatc accccaggcg ttatcgattt tatctgtacg tccctgattg
      301 cggttattct gacgattaag ctatttctgc tgattaatca gtttgaaaaa cagcagatta
      361 aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg cattatcaaa atcaccatta
      421 ttgtagtgct tgttctgctt tatggcgaac atttcggcat gagcctttct ggcttgctga
      481 cctttggtgg tattggtggt ctggctgtcg gtatggccgg taaagatatt ctgagtaatt
      541 tcttttccgg gatcatgctc tatttcgacc gtcctttcag tattggcgac tggatccgtt
      601 caccggacag aaatatcgaa ggtacagtag cggaaattgg ctggcgaatt accaaaatta
      661 cgacctttga taatcgtcca ttgtacgtac cgaactcgct gttttcgtcg atcagcgtag
      721 aaaacccagg acgaatgacc aaccgccgca ttaccacgac cattggttta cgttatgagg
      781 atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga gatgctgaaa aatcacccgg
      841 ccatcgacca gcgacaaacc ttactggttt atttcaacca gtttgctgac tcgtcattga
      901 atattatggt ttattgcttt accaaaacca cggtatgggc tgagtggctt gctgcacagc
      961 aagacgttta tttgaagatt atcgatattg tacagtcaca cggcgcggac tttgccttcc
     1021 ccagccagac gctgtatatg gataacatta caccgccgga acaggggcgc taaatcagac
     1081 atcactggcg ttatccacaa gggtaacgcc agttcccatc acatcaatgc ttcacaatat
     1141 acatagtccg actgtacgct acatcttcag ggttattaat gggataacct ttcagccacg
     1201 gtttgattaa tcgtccattg gtatattgat aaattggtgc aatcggtgct tgctccatga
     1261 ggattttttc tgccgcgttg taatcggcat tacgcgcttt aacggtattt tccgtcgatg
     1321 cctgggccag aactttgtcg tatgccggat tgttaaagcg tgaaatattt cctgaatgca
     1381 ttgacgttaa taatgtcagg aaagtggagg gttcattata atcccccacc catgaggcgc
     1441 ggataacatc aaaattgccg gtgttacggc tatcgatata ggttttccat tcctgatttt
     1501 gcaatttaac atctacgcca aggttctttt tccacatcga tgctacagca atcgcaattt
     1561 tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt cagcggtttt tgcggaccat
     1621 aaccagctgc gctcaacaaa gtttttgcct gcgcattcag ttcttcctga ctcatttgtt
     1681 caaacggcga aggttccggc gtaaatccgg cggtaacatc tggtataaaa tgccacgctg
     1741 gcttttcgcc cgtccccaac actttttcgg tcatcaggcg gcgatctatc gtcatactta
     1801 atgccaggcg aacgcgctga tctgccgtcg gccctttttg cgtgttaaag gcataataat
     1861 aggtcccgag ctgcggtggc gtataaacct gccccggaat atccttcaac agcttctgat
     1921 acatattttt cgggaaggat tcggtgatat caatatcccc cgcgaggtaa cgcttagtgg
     1981 ctgcggattc ctgattaatt ggcaggaagg tcactttttg cagcaccgtt ttggcgttat
     2041 cccaataatg ggtattcggt accacgacca gtttttcatt gactacgcgc tctttaagaa
     2101 cataagcgcc attgccgatc agatttccgg gtttcgtcca ctctttaccg ctttctacgt
     2161 tggctttttg caccgggaag aaggcaaagt tagcggttaa attcacaaac cacggcaacg
     2221 gtttatcaag ctgaattttc aaagtatggg catcaactgc ggtgacgcca agctgatcag
     2281 gcgtagcttt accatcaata atcgcctgtg cgttgttgat tcccgccagc gcggcaaacc
     2341 atgcaaatgg cgacaatgtt tttgggtcca ccagacgttg ccagctgtag acaaaatctt
     2401 gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc gcgcagggta aaagtccaga
     2461 tacggttgtc attacttttc cattgagtcg cgacgccggg gacaatctcc cctttttcgt
     2521 tctgattcac cagaccttca aacagatcgc gaatgacctg aatctctggc aggcccacgg
     2581 ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc tcctgcttct
     2641 ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg ctgctgacca
     2701 acagcgcaca acacgtaact gaaacagagt gcttcataag tttccctcta accgacatta
     2761 aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc catcaaaaaa
     2821 gtgacaaagg aagcatcaaa tgttttaagt atcaatatat caaatcagat ttagtgtaaa
     2881 taatagacgc caaaatgtgg caaagcacat agaacaccga acactacgtt cgggcttcgc
     2941 acgttcaaac cagttttcgg tatcataatc agcatctgtc gccattgtac gaagaataaa
     3001 aaataaccaa aacgttgaaa ggaactgaga atacgttaag agaagaatta aattaatgta
     3061 tcagcaagta gtgctaaaca gcacctttat acttcagcct atctataaat aatgagaatg
     3121 ccgagccagc atgtcgacgg tgtggatagt acaggtgata accgggtaaa tctggagtg
//
LOCUS       DAJVPP010000208         3031 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVPP010000208 DAJVPP010000000
VERSION     DAJVPP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.215"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQM34_004028"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQM34_004028"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1454241.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQM34_004029"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQM34_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1454242.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..>3031)
                     /gene="bcsE"
                     /locus_tag="OQM34_004030"
     CDS             complement(1963..>3031)
                     /gene="bcsE"
                     /locus_tag="OQM34_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1454243.1"
                     /translation="FGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLV
                     QSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSL
                     QQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPL
                     SRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLP
                     AHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALN
                     HIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDG
                     RHWRRIPEPMRLLDDAVERSS"
BASE COUNT          758 a          780 c          802 g          691 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg tagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa a
//
LOCUS       DAJVPP010000209         2900 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVPP010000209 DAJVPP010000000
VERSION     DAJVPP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.216"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..689)
                     /gene="ipaH9.8"
                     /locus_tag="OQM34_004031"
     CDS             complement(<1..689)
                     /gene="ipaH9.8"
                     /locus_tag="OQM34_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1454244.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSS"
     gene            complement(951..1046)
                     /locus_tag="OQM34_004032"
                     /pseudo
     CDS             complement(951..1046)
                     /locus_tag="OQM34_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1082..1387)
                     /gene="ccdB"
                     /locus_tag="OQM34_004033"
     CDS             complement(1082..1387)
                     /gene="ccdB"
                     /locus_tag="OQM34_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCS1454245.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1389..1607)
                     /gene="ccdA"
                     /locus_tag="OQM34_004034"
     CDS             complement(1389..1607)
                     /gene="ccdA"
                     /locus_tag="OQM34_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCS1454246.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2143..2900)
                     /locus_tag="OQM34_004035"
                     /pseudo
     CDS             complement(2143..2900)
                     /locus_tag="OQM34_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          730 a          692 c          691 g          787 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gcatgggatg ataatgggtt atcactaata
       61 tgtattgaac attcattcct cagattaaga atgctttccg ggatatgact tatctgattt
      121 ctatcaaaaa aatactctct gacaacggga ttatttccct cagaaaatgc tggtagctca
      181 gtgaggaaat tacgggtcgc tctgagattt ttaagagcct gtgggagcga tggtaaggag
      241 actatttcgt tatagctgat attcatagtc aataacgaat cgggtaaagc tggcaggttt
      301 tccagctcat tgtgttgtac ctgaagtgac tccagcgcag gaggtagcac gggcaattct
      361 gataatttat tgctggcgga atataatttt tttagcgtaa caggcagttc aggtaggtta
      421 gttaattgat tatatgatac attgagcagc gttatctgag ctggtaagtt gtcaggtagc
      481 gaggacagat ttaaacggtc cagtcgaagt tcatcggaat tattgataag acattcttta
      541 agtcgggaga cagcctcatc acgctcttca ccggggagcg cttgtttttc ccatttatcc
      601 catgctgaaa agtaatcagc atatgtaccg gaaatagtgt tataaaaaga attttggggc
      661 aatgaaaagt tattatttat cggtaacatg atgggcatcc agttaaagcc ggtggacaaa
      721 tggctttgtt aaaagaaata agtaggagtt tttatgaagt tcattccatg aaatgaactt
      781 cagcactttt ttattataat aaggataaac aaaattggct gtaaaaaaac gatactgttt
      841 cagttaaccg tgaatggcct gtttgcaccg tattgttctt gtttttcggt tagttaaaca
      901 tcgtacttca tatttgaacg gtaagcggct cgtcagaacc gtattgatat ttactgagag
      961 gtcagatcaa ctttccaagg caacagatcg cgtacccggt ttgccggcca gtcctggata
     1021 tgctcaatga cgtaacgcag ccattttgtc aacgccacga tttttgaccc tagcctgaca
     1081 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
     1141 gatcagccac ttcttccccg ataacgaaga tcggcacact ggccatatca gtggtcatca
     1201 tgcgccagct ttcatccccg acatgcacca ccgggtaaag ctcacgggag actttatctg
     1261 acagcaggcg ggcactggcc agggggatca ccatccgtcg tccgggcgtg tcaataatgt
     1321 cactctgtac atccacaaac agacgataac gactctctct tttataggcg taaaccttaa
     1381 actgcatttc accagtccct gttctcatcg gcaaaagagc cgttcatttc aataaaacgg
     1441 gcgatctcag ccatcccctg ctgattttca gcctgccagc gttccgcacg cagacggcgg
     1501 gcctcttttt gcatggccgt atttaccagg cctgagatat tcacgtttgc ggatttaagt
     1561 aactgatagc tgtcactgtc tatagtgaca gtaatacgct gcttcatgat aagccctcaa
     1621 aaacatactt atgatatgta tatgagtata tattaatggt acaaaccatc aatatccata
     1681 aaaagtacac cattaattcg ctataagttt ttaatacaat tttttctgct gcgatttttt
     1741 tatactataa ttagcatact tataacaccg taagtaaatt acatgaaagc aggcacagcg
     1801 tgatccgatt tttctgcgta taacgatttt ccggaagtgt aatacggttg cgccccgggc
     1861 cgggcaccct tttctgttgt tgtgtacggg cccacagaga atggtaccgg aggcgggctc
     1921 agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta ctgacacggg tattggtgac
     1981 tggtgatgaa gtactgcccc gggtatgatt tcaggctgat tcaggttctg attctctgtc
     2041 agtccgcgtg gttgctggcc tggctgattt cctgttgtca ggcgttcaac aaccgactgc
     2101 aggttatagc ccggttcacg aacacctgac ggcaggtatc actcacccgg tgaaggtaaa
     2161 tccctcaaga ggcagcaact ctgccagtcg ctcctccggc cattccggca gacgcgtcag
     2221 gacgtccgtc agccaggcat gtggctccag accattacgt tttgctgttt ccagaaggct
     2281 cattatctgt gccgcccggt ttccggccat cagtgaaccg gcgaacagcc acgctttccg
     2341 tcccataaca accgggcgaa tggcacgctc gcagatattg ttgtccaggg gcaccgctcc
     2401 gtcacccagg aagcagctca gttcatcgcg ccggttcaga atatagttaa tcgccttatt
     2461 taatggcccg tcgggcggac agcacggctc ctgctcttca agccaggaaa acaggtcatt
     2521 cactatcggc cgggagtacc gttatcgcca ctgccgtatt ttttccacag ggcgctcacg
     2581 aatgagtttc tcaatgcggt aaagacccgc gatttttctc agctccacgc ctgcacgggg
     2641 ctccctgctg gccttataca gatttgcgaa gccgcgccct gcgtgactcc agcaacaggc
     2701 tgatgtgatg ccggggtgat tatccgccag agatttgtac acgctgtatc cgtcactcac
     2761 cagcgtactg ccacaccagc actgcagcca ggtctcaggg tatctgaggg cccgtccggt
     2821 ctgcgagtca aaacagacga ccggcggacc gcttctttca ccgctgacgt aagcacacag
     2881 ccagccggag caggatttac
//
LOCUS       DAJVPP010000210         2888 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVPP010000210 DAJVPP010000000
VERSION     DAJVPP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.217"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            224..985
                     /locus_tag="OQM34_004036"
     CDS             224..985
                     /locus_tag="OQM34_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1454247.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(1092..1592)
                     /locus_tag="OQM34_004037"
     CDS             complement(1092..1592)
                     /locus_tag="OQM34_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454248.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(1743..>2888)
                     /locus_tag="OQM34_004038"
     CDS             complement(1743..>2888)
                     /locus_tag="OQM34_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1454249.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPRATGVNVNGWPGVSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT          860 a          647 c          524 g          857 t
ORIGIN      
        1 attttgtcat tttagcctat tcctggtgga actgacacaa gaaactaaaa tacaaatata
       61 accctgaaaa agtcatataa aacttatatc cacagaataa aaaatcaaac atgtcagttt
      121 tatcaatata aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa
      181 gcgcaccaca tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac
      241 tcaggagcta cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat
      301 gatacctgat gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag
      361 agtgacagag tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt
      421 tgaactcatc tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa
      481 aagagactta aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga
      541 cgtgttaatt atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt
      601 tgaagaaaat ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga
      661 agatctttgt tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa
      721 gattgcaaaa atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag
      781 atttttaaca gatatactct ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg
      841 tagatccgtt aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca
      901 taaagttcgt gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta
      961 taaagcgcta attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt
     1021 ctatgtcttt ttttataagt taaaaaaggc gggatatcaa accccgccat gaaattaact
     1081 aatcatctgc attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc
     1141 tcaacaacct gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt
     1201 ttcagcatca tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc
     1261 tcaagcgttg tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact
     1321 gcatccgctg aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg
     1381 tggctctgcc catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac
     1441 gtaccgctat ataccccttt cactggttga atggtattat taacaacgat aggcttggtc
     1501 aaagtaagat tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca
     1561 aaagctgtcc ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga
     1621 ttttcattta cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat
     1681 gcttccacca aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga
     1741 aactataaac atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt
     1801 tggtattttg cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct
     1861 tccgcactca agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt
     1921 tcagtgtctt tgctataact aatcacagcg ccgaagggga cgggtttacc ctctttctgt
     1981 tttaaggtta ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct
     2041 ccagctgtcg gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt
     2101 ttttcatcca gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca
     2161 cgaaaatcag tactcactcc cggccagcca ttaacgttaa cccccgttgc tctgggtgcc
     2221 tctataaggg ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata
     2281 agtatcccac cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg
     2341 ctactaaact gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc
     2401 ggttgtgact gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta
     2461 ttctgacctg ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt
     2521 gaagacgcat aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt
     2581 aatttccgat ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg
     2641 gagaatgtta tgcctctcca taccgtacta taactcaggc tggtagaccg cgtgtttcct
     2701 gtgccccggg aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg
     2761 ggctgggata aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt
     2821 tcatcctgtg cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg
     2881 gttataga
//
LOCUS       DAJVPP010000211         2877 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVPP010000211 DAJVPP010000000
VERSION     DAJVPP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.218"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(371..1066)
                     /locus_tag="OQM34_004039"
     CDS             complement(371..1066)
                     /locus_tag="OQM34_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCS1454250.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1059..2486)
                     /locus_tag="OQM34_004040"
     CDS             complement(1059..2486)
                     /locus_tag="OQM34_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCS1454251.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2497..>2877)
                     /locus_tag="OQM34_004041"
     CDS             complement(2497..>2877)
                     /locus_tag="OQM34_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS1454252.1"
                     /translation="FIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNV
                     RGLELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLN
                     ISGRLQREGQKVKVMHIAEVLMSR"
BASE COUNT          715 a          809 c          613 g          740 t
ORIGIN      
        1 aaataaaaaa taaataccct gctgccattt gcctggctct gggatttttt ctaagcattg
       61 taatttctgt aatgttttgc cttgtcaaaa aaatggtaga tgaataccaa caaaactctg
      121 ggcagtaata aacagcattc tgatagagag tgcctgtata aagctgaagt gacaatgacc
      181 gggaagggaa cccgttttca ggaatggatg cctgtctttt tcactgtaaa tatcctggca
      241 ttactaatgc ctggatattt acagaagagc agcataacca tgacggaacg gggttaagtc
      301 aggttgtcct taacgctatt ttcaacctcg tatgccttct tcaggtttat gttcagatct
      361 catatctctc tcagcaatcc tcaataatca gatacaccgc tttcaccgga ccatgaaccc
      421 cgacgacttt gataagctca atatccgccg ttgagctggg accactgatg atgttaatac
      481 atgacggcat tcgttcaccg gcctgcgctt tctgatgcaa tttttctgcg agttgcgcta
      541 cacgcggcag gattgtgctt ttacgcagga taaaaagaga agattccggg agcaggctca
      601 atgaacgccc gcgctcggcg gcggaaaaaa gaaccacgcc tcccgattcg gttaaaccat
      661 attcagcata cacgacaccc actttagcct gctctgcctg cgagatattc tcggcacctt
      721 tcgccggatc ccaaacaacg gcattgcatt cctgctgcaa acgttcacta atccccaatt
      781 cctccagcct cgtgtcaccg ctaatcacga ccgactgatc tcccagctct ttacacagac
      841 gtattgcagc ttctgccgcc ttcgcctcgc tggtcagctc acagcgcgtc aacataacat
      901 cgctggcaaa ctgaataaac gcgtcacagc gctgctgttg gttaagttgg gtaagccgct
      961 cgttagcata gttgttaagc ggcgcatctt ctgcttgcgg ttcaagtcgc agcgggcgac
     1021 ccagtgcctg agcaacgtta ttcaaaaatt cgctccgatt atccattctt tttctcctgc
     1081 gcctgatgtt tcttaaacca actacggaaa ctctctccgt cagcttcagg aagatcgcgt
     1141 gcttccatcc agtcgctaat cgcgccaaat ttgagtggtg ttttgccgcc attgataaac
     1201 cagcttgccg catgagcgcc ggccatcatc ccgactttcc acaatcctgg atgactattg
     1261 gcataagcga acatttttat cgcccgttgc tctgcttttg cggtgatccc tttttcagcc
     1321 atcacccgac gatgacgcaa aatcagtttt gacagcggaa tacgcaccgg acacacgctg
     1381 tcacaagcag tgcataaaga gcaggcgtag ggtaaatctt taaaatcttt atagccgcca
     1441 agtagcggag aaatcaccgc accaattggc cctggataaa tagagccata tccatgaccg
     1501 ccaatatggc gatatgccgg acaagtattc atacaagccc cgcagcgaat acagcgcagc
     1561 acatcccgaa attcagaggc cagcacctca gaacgcccgt tatcgacaat aaccagatga
     1621 aactcttcag gaccatcaac gtgccccgct tcgcgcggcc ctgtcagcca ggtgttgtat
     1681 cccgtcaaac gtgcaccaac ggcactgcgc gccagcatgg tgatcaatac atctacctcg
     1741 gcaaacgtgg gggcaatacg ctccattccc atcactgcaa tatgcgtttt aggcagcgtg
     1801 gtacacattc gcgcattacc ttcattggtc accaggcata ccgaaccggt ctctgccacc
     1861 gcgaaattac agccggtaat acctatttca gcactgagga aatcttcacg gattttttgc
     1921 cggatgaata aggtcatcgc ttcaggcgtt tccggcccct catagcccag acgttcgtgt
     1981 agcactcgac ggatctgatg gcgatcttta tgaattgccg ggaccacaac atgagatggc
     2041 ggatcttgat ccagctggag aatatattca cccagatcgg tttcaatcac ctgaatgcca
     2101 gcatcctgca acacatgatt gacaccaatc tcttcggtca ccatcgattt agatttcacc
     2161 accttccggg catttttgcg ttgggcaacc tgtaaaatgt agcgggtagc gtcttctttg
     2221 gtttttgcaa aatagacgtg accgccgttt tgcgtcactt tttctgagag ctggtacaga
     2281 taagcgtcga gattactcag aacatgatca cgtatctggg cggcccgatc gcgccactcc
     2341 tcccagtgcc ccaattcatc gaccattttt tgccgatttg ccccaatacg ctgctgcgcg
     2401 cttgccaccg ctttgcgcat gatcggatct tcaatttgct gacggatgcg tgtcttaaaa
     2461 tctgtattac tggttttgat cgacatcttt atatcctcag cggctcatca acacttcagc
     2521 aatatgcatc actttgactt tctgcccttc ccgttgtaat cgcccactga tgttcagcag
     2581 gcaactcacg tcagcaccaa ttaaatactc agggcggact tccatcaggt gcacaacctt
     2641 ttctttcacc atctcgccgg atatttcggc cattttgacc gagaacgtgc cgccaaatcc
     2701 gcagcaggta tcctgttcag caaaggtcaa cagctccagt ccacgcacat ttttcagcag
     2761 cgtaagtggc tcgtccttca ctcccagctt acgggccagg ctacaagatg ggtgatacac
     2821 agctctccct tgcaaactgg cacctacatc gactacccct aatttattaa caataaa
//
LOCUS       DAJVPP010000212         2792 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVPP010000212 DAJVPP010000000
VERSION     DAJVPP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.219"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..488
                     /locus_tag="OQM34_004042"
     CDS             <1..488
                     /locus_tag="OQM34_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1454253.1"
                     /translation="LKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVN
                     CPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRIDDAES
                     GNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNVTLDY
                     R"
     gene            568..1263
                     /locus_tag="OQM34_004043"
     CDS             568..1263
                     /locus_tag="OQM34_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1454254.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1292..>2792
                     /locus_tag="OQM34_004044"
     CDS             1292..>2792
                     /locus_tag="OQM34_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1454255.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEI"
BASE COUNT          807 a          658 c          624 g          703 t
ORIGIN      
        1 ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt gctcttggtc
       61 actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt ccattcacta
      121 ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac gttgaagcgt
      181 ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc cctaatctgg
      241 caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt attgacgatg
      301 cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg atttatcaga
      361 tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac gtatcaaccg
      421 ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta acgctggatt
      481 accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc cttcctcttt
      541 ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt gatattaacc
      601 ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt acagcgaacg
      661 cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa caaaggcgca
      721 gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag ccgtgcccca
      781 tttattataa ccccaccgct atttcgtctt gaggctggcg atgactcatc actgcgaatt
      841 attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat taatgttcgt
      901 gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac gctggtattt
      961 aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt aaacgatgcg
     1021 tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa cccaactgaa
     1081 tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag caaaattgaa
     1141 tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg aaagaacgtg
     1201 aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg tccactgcaa
     1261 taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc agggacataa
     1321 acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg tgctcactgg
     1381 cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc ccattatgct
     1441 ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa aaaaatacgc
     1501 acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg tttcacagaa
     1561 aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta cggtagaaca
     1621 actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa aagatgacga
     1681 tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat ttgatttcaa
     1741 tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg caagaggtta
     1801 cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact actcgtttac
     1861 aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc tgaatatgca
     1921 aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga cacgcaacga
     1981 tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca aggcgttgaa
     2041 gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca gctacgcctt
     2101 tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc gcggatttgc
     2161 cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc aaaatggtta
     2221 tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc tctacccctc
     2281 ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac aacgtcgctt
     2341 tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa aatatagcgc
     2401 gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg aatttgctga
     2461 agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc tgctcggttc
     2521 tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac tgggcgcgtt
     2581 gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt ttcatggcta
     2641 tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata tcgctgttag
     2701 ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata cccgcaattg
     2761 gggctataac agtcgccaaa aaagtgaaat tc
//
LOCUS       DAJVPP010000213         2675 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVPP010000213 DAJVPP010000000
VERSION     DAJVPP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.220"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..674)
                     /locus_tag="OQM34_004045"
                     /pseudo
     CDS             complement(1..674)
                     /locus_tag="OQM34_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(681..1470)
                     /locus_tag="OQM34_004046"
                     /pseudo
     CDS             complement(681..1470)
                     /locus_tag="OQM34_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1548..1970)
                     /locus_tag="OQM34_004047"
     CDS             complement(1548..1970)
                     /locus_tag="OQM34_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454256.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            complement(1954..2181)
                     /locus_tag="OQM34_004048"
     CDS             complement(1954..2181)
                     /locus_tag="OQM34_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1454257.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            2258..2665
                     /locus_tag="OQM34_004049"
     CDS             2258..2665
                     /locus_tag="OQM34_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1454258.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
BASE COUNT          632 a          749 c          589 g          705 t
ORIGIN      
        1 ttcatgacca tgcgatccat cacccgctcg ccgagcaatg ttttcatcac ctcatagttc
       61 aggtttgtca gcatccccac gctgcgcatc gacgctgtcc ggcgatcaac aatctggtgc
      121 agcaccacct gctcgttttt cgtctcgcgc tgaatgccaa tttcatcaag aaccagcaga
      181 tccacttcgc acagctcccg caaaaatttt tcgcctgact gcccgtcgtc atagctggcg
      241 tgcagggcgc tcataacatc agccacggta accacaatca ctgtctgacc gtctttcagc
      301 aggcgattcc cgatagctgc cgctaagtga ttctacccgg tatcaggttt tccgctgaac
      361 gcaaaatttg tacacccggt catcagttca tcggcgatgg atttcgcctg gcttaacgcg
      421 tatcgctggc cgtcgttctg cacctggtaa ttcgcaaacg agcatttacg gtgcaacggc
      481 tggatgcctg agcgattcag aattttttcc acccgcaact ggcgattcag gcggttgatc
      541 tcctcgctac gtttctggcc ttcagcaagt tgccactcgc gccactccgc taccgttctg
      601 aatggggcgg ttacatgttg cggggtcagt cggcggatgc gttccagaac gccgcctgtc
      661 gcaatatttt tcatggtccg ttaccccctg aagcctggcg ggatcgcact gtccggcaac
      721 gagacggtgt taacctgtcg gagcaacgtc tcaggccgaa cacctttcgg cgcgaacagg
      781 ccctggtatt cattggcgat gctgtgtcga atcacctgct caggtgtaaa accctgctga
      841 cggaattttt ccagttcccg tatcgccctg ctccgttcga atcggttttc gcaatgcctg
      901 tctgaaccgg acccactcat gccagagtgt ttccggcaac caatcgggca gctcaatagc
      961 ctccggctcg aattttttag acgctcgttt ttggcgaggg ggatttaggg ggagatcagt
     1021 atttagatct tcctcttcct cttcctcttc ctcttccact ggtaacgctt tttgatccgt
     1081 ttgtgtaacg ctggcagcgt taccttttcg tttcagttcg cgtatttttg ttactcgctc
     1141 gtttgtaacc gcccgttttt tagagctttt tccgttatga cgttcaaagt taggtagaga
     1201 aagcccaccg tcattttcga ccagccatcc aacctgaatt aacgcatcag caaaaccaga
     1261 cataaaagtg atgcgatcta ttgcactttt tgtaacgccg cgagcgttac aatctgcatt
     1321 accgtctatc atttgttgat ccgcccatgc ccagaagcga ataaccttcc ctaatgcggc
     1381 atctggatca atattcagaa tctcagcaag cctgaatatt tccggcttat ccggcgtaat
     1441 aacctcgagc tttatccagt ttgaagccat ttgttttcac cttgtaacgc tcgcagcgtt
     1501 acatttaact gataccgaac aaaacaatcc ggcacgatta atttcaatca atgcactacg
     1561 acagaatcgc cgggcgaccc accaccgctg aaatgtgctt tccggtaaac ggcctggaca
     1621 gcatcatcat gcgcatcaat tgccgtactc aacgcttcct gcgccgccag taatgcacgg
     1681 cgttccaggg tatcgaagat acagagtcgg tgacgcagct cacgcggaag aattgccaga
     1741 accgcaggga tcagtttctg aattttttcc ctttgcgctt tcgtttcacc tttcaaccaa
     1801 cggtgataga tattctgctg attgttccag tccttgcctg gtaccagaga caattcgccg
     1861 cccccctgcc gcagatattc ttcagtaatt gcgttagcga cccacgcctg ccctttttcg
     1921 gctgccaagg ctaacagcac tgattcgatg tgctcatgcc tgattttcat gaatcacctt
     1981 cccaggtcgt tttgccttac gatattcgtc ataaactttg gggtcgtact gaagttcccc
     2041 gccagatgcc tcttgcaggc gcatcgcgcg accttcagga accagttccc cccatgcagc
     2101 aatgcttgcc agcctaactc ctgcggcatt ggcaagcttt gttttgctgc caaaaaacgc
     2161 tatagcatca attttcaaca tatcgagctc cttagatttt cctaaggaaa ccaggccgta
     2221 gagaaaccta agtcaagaaa aattagaatt ccctaatatg aaaaacgaaa ccttcggtgc
     2281 tcgcctctta cataggcgta aaaaattaaa actgtctcag gccgcattag gtaagctggt
     2341 caaagtggct cacgtaacaa tttctcaatg ggaaagagat gaaacacagc cggcggggaa
     2401 aagattattc gcactgagcc aagcgcttca gtgctcaccg acttggcttc tttttgggga
     2461 tgaagataaa caaccaggcg aaccgatccc ggataatcag ccagccattc tgacagaaga
     2521 tcaaaaagag ttacttcaac tgttcgacgc actgcctgag tcagagcaaa aggccctgtt
     2581 gtcagagatg cgtgctcgag ttgaaaattt caacaaactt tttgaagaac tactcaaagc
     2641 tcgcaaaaga agcgcaaaca aataatcccc ttttt
//
LOCUS       DAJVPP010000214         2570 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVPP010000214 DAJVPP010000000
VERSION     DAJVPP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.221"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..885
                     /locus_tag="OQM34_004050"
     CDS             <1..885
                     /locus_tag="OQM34_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCS1454259.1"
                     /translation="PIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGA
                     VVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGG
                     LLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVV
                     ALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANT
                     LAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKG
                     LWLRRKAKAAQQELVHEH"
     gene            875..1945
                     /locus_tag="OQM34_004051"
     CDS             875..1945
                     /locus_tag="OQM34_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1454260.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPSVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            1945..>2570
                     /locus_tag="OQM34_004052"
     CDS             1945..>2570
                     /locus_tag="OQM34_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCS1454261.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT          558 a          673 c          717 g          622 t
ORIGIN      
        1 cccatcatgg gcgcttatat tgcctcgtcg attgccgata aaccggcttt cgctccggcg
       61 tttctggttt gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga
      121 ttcctcggcg cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgcaaa
      181 gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa tgctgatccc gttcgttact
      241 ctgctggttt tcggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg
      301 ggcgggctgc tccacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt
      361 ctggtcggtg cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc
      421 ttctgcttct cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc
      481 gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca ttgcgcccaa actgtttacc
      541 cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg gtgccacagt cgcaaccgaa
      601 ccggctatac cttacgctct ggcagctccg ttgccgatga ttaccgccaa tacgctggcg
      661 ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca aacgtctggc acctggtttg
      721 ggtatctttg acccgctgat cggcctgatg tcgccggtag gttcgtttta tctggtgctg
      781 gcgattggcc tcgcgctgaa catttcattc attatcgtcc tgaaaggatt gtggttacgc
      841 cgcaaagcga aagccgcgca acaggagctt gtccatgaac attgagttac tgcaacagtt
      901 gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt cgcgacatcc tgataaacac
      961 gctggaacct tgcgttaatg aaatcacctt tgatggtctg ggcagctttg ttgcccgtaa
     1021 gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg gatgaagtcg gctttatggt
     1081 cacccacatc gacgagagcg gttttctgcg tttcaccacc attggtggct ggtggaatca
     1141 gtcgatgctc aaccatcggg taaccatacg cacacacaag ggagtgaaaa tccctagtgt
     1201 gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa aggcaacagc cgctgtcatt
     1261 tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat gaggtggaaa aacgcggtgt
     1321 ggcgatgggc gattttatta gcccggaagc caattttgcc tgctggggcg aagataaagt
     1381 ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg atggctgaac tattgcagac
     1441 ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc gtggaagaag aagttgggct
     1501 acgcggggca caaacctcgg cggaacacat taaaccggat gtagtgatcg tgctggatac
     1561 cgctgtcgca ggcgatgttc cgggcattga taacattaaa tacccgctga aactgggcca
     1621 ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc aaccagaaac tggtagcagc
     1681 gttaaaaaac tgtgccgcac ataacgattt accgctgcaa ttttccacca tgaaaaccgg
     1741 agcgacggat ggcgggcgct acaacgtgat gggcggcggg cgtccggttg ttgcgctgtg
     1801 cctgccaacc cgctatctgc acgctaacag cggcatgatt tcaaaagccg attacgatgc
     1861 tctgctcacg ttgatacggg gttttctgac gaccttaacc gcggagaaag tcaacgcgtt
     1921 tagccagttc cgtcaggtgg attaatgcta aacgaacgcc agttaaagat tgtcgatctg
     1981 ctggagcaac agccgcgcac gcctggtgag ctggcgcaac agactggcgt ttcaggcagg
     2041 accatcctgc gtgatattga ctatctcaac tttaccctta acggcaaagc ccgcattttc
     2101 gccagtggca gtgcgggcta tcagctggaa atcttcgagc gccgcagctt ttttcagttg
     2161 ctgcaaaagc acgataacga cgatcggctg ctggcgctgt tattactgaa tactttcact
     2221 ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa cgtgggtagc agagcgtctg
     2281 ccccagttaa aacagcgtta tgaacgcact tgttgcctgg ccagccgccc tggtttgggc
     2341 catttcattg atgagacaga agagaaactc gttatcttgc tggcgaactt gctgcgcaaa
     2401 gatccgtttt taattccgct ggcgggcata acacgagaca accttcagca tttatccacg
     2461 gcctgcgaca accaacaccg ctggccgctc atacagggtg attatctctc cagcctgatt
     2521 ctggcgattt acgccctgcg taatcaactg accgatgagt ggccgcaata
//
LOCUS       DAJVPP010000215         2534 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVPP010000215 DAJVPP010000000
VERSION     DAJVPP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.222"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..557
                     /locus_tag="OQM34_004053"
     CDS             <1..557
                     /locus_tag="OQM34_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1454262.1"
                     /translation="DLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELL
                     MCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSER
                     KVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGAREL
                     NLPSVYGDAAEWNIAELRREMSHS"
     gene            515..1192
                     /gene="bglJ"
                     /locus_tag="OQM34_004054"
     CDS             515..1192
                     /gene="bglJ"
                     /locus_tag="OQM34_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCS1454263.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1230..2018)
                     /gene="fhuF"
                     /locus_tag="OQM34_004055"
     CDS             complement(1230..2018)
                     /gene="fhuF"
                     /locus_tag="OQM34_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1454264.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2159..2395
                     /locus_tag="OQM34_004056"
     CDS             2159..2395
                     /locus_tag="OQM34_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1454265.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2434..2520)
                     /locus_tag="OQM34_004057"
     tRNA            complement(2434..2520)
                     /locus_tag="OQM34_004057"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2484..2486),aa:Leu,seq:cag)
BASE COUNT          650 a          566 c          649 g          669 t
ORIGIN      
        1 ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat tattctttaa
       61 tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg tattcccagg
      121 ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa cccattgaga
      181 atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc gccatgctga
      241 cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt ctcactcttt
      301 cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag atagcttcat
      361 tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg cgtcgactgg
      421 caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt gcaagagaac
      481 ttaacttgcc ttctgtttat ggagatgccg cagaatggaa catagccgaa ttaagaagag
      541 aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga gtttattcag
      601 aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt catttcagga
      661 tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca tgagaagtga
      721 gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc gtacccggcg
      781 tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt cgccatcacc
      841 gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg aactcttttt
      901 gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt acattaacca
      961 aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc gtggctactc
     1021 aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag cgcataaatt
     1081 taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg ccgcagatat
     1141 tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt aatccgcatg
     1201 atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc acattgctgc
     1261 acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc gtcgcgcagt
     1321 accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc aaaaaagagg
     1381 gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc agtgagatac
     1441 cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc tccggtcgct
     1501 tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat tcgctgctgc
     1561 ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca ggcgacgcgt
     1621 ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc tttttcctgc
     1681 gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg tgcccatagt
     1741 gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat atgatcggaa
     1801 tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag cgtcatggcg
     1861 ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg atgtttggcg
     1921 atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg cgttcgccag
     1981 atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt aatctaaatg
     2041 ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc tctggcttgc
     2101 aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag atgaaaccat
     2161 gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc tgattgcagg
     2221 gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga tgggattagt
     2281 attgacgccg gtgatgctgt atcacaaaca attgcggcat tacattttgc taccatcgtg
     2341 cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg gatgacaaaa
     2401 cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga cttgaacccc
     2461 cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg ccaccttcgc
     2521 acagtcatct tact
//
LOCUS       DAJVPP010000216         2497 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJVPP010000216 DAJVPP010000000
VERSION     DAJVPP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.223"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..454
                     /locus_tag="OQM34_004058"
     CDS             <1..454
                     /locus_tag="OQM34_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1454266.1"
                     /translation="LNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTPIGIQVMSLS
                     YVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQADAIRTKAQ
                     AEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            498..1247
                     /locus_tag="OQM34_004059"
     CDS             498..1247
                     /locus_tag="OQM34_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS1454267.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            1244..1813
                     /locus_tag="OQM34_004060"
     CDS             1244..1813
                     /locus_tag="OQM34_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454268.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            1937..2179
                     /locus_tag="OQM34_004061"
     CDS             1937..2179
                     /locus_tag="OQM34_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1454269.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLVEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            2183..2329
                     /locus_tag="OQM34_004062"
     CDS             2183..2329
                     /locus_tag="OQM34_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454270.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEDWCHCRG
                     LSVV"
     gene            2338..>2497
                     /gene="xisR"
                     /locus_tag="OQM34_004063"
     CDS             2338..>2497
                     /gene="xisR"
                     /locus_tag="OQM34_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCS1454271.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          682 a          600 c          663 g          552 t
ORIGIN      
        1 actcaaccga ctggccagca aaatgaccac cgacaaattt atcgacggcg gcaagtctga
       61 gctgctggat gcagctctta aagacattca ggaagaaatg acgcccatcg gtattcaggt
      121 aatgagcctc tcatatgtgg gtaagccgga gtacccgcct actgttatcg acagcattaa
      181 tgccaaagtc acggcgaacc agaaaaccct gcaatgcgag caggaagtaa aacagcgcga
      241 agcggaagcc aacatgttgc gcgcggaagc tgccggacag gcagatgcga ttcgcacaaa
      301 agcccaggcc gaagccgatg ctattcgttt acgcggtgaa gctctgcgcc agaacccaaa
      361 cgttatggag ctggaagcca tcaacaagtg gaacggtaca ctgccgcaat acatgaccag
      421 cggtgccaac acaccattta ttcaggttaa gtaatacata tgcccggtat tacacgccgg
      481 gctgtctgga gataaaaatg aatactgtaa ccatcaacaa caaacagttt ccggtaatcg
      541 aatatcgcgg tcagcgtgtt gtgacgctgg cgatgattga tgaagtccac cagcgaccgg
      601 aaggaaccgc gcgtgcagca ttcaaccgaa accgtgagca tttcatcagc ggtgtggatt
      661 atgccgaatt aggtgcggac gtaatacgta cggacctccc ggaaggaaca ttctctaaat
      721 ttgcaccatc ggggattgta ctttttgaat caggttacct gatgttgacg aagccattca
      781 atgacgccct tgcctggcag gttcagcgcg aactggttaa cagctacttc cgcactcgca
      841 caccgctaac ggaaatcgag atgatcgctg cgatagccgc cgacgctgtt cgtcaacaga
      901 agcgcctgaa tcatgtggaa gaacagctcg aaacagtcac agaagcggtg gaaaccatca
      961 aacgcggaaa tatgcgcgcc ggatatgtcg gttaccgcca ggtggtagcc aaaagtggaa
     1021 tgagtgacac caagtgtcgg aatctggtga acgcatatca aattcctaca gacacccacg
     1081 aattcatgac tccggacggt ctgttgtctc gcagggctat cgtcgagctt gagccattta
     1141 tggcggcgtt ccaccaaatg atgtcagagg ctgacccacg cggcacacgc tggtatcacc
     1201 cgaagatggg gcttttccag gtgatcggat gggaggataa agcatgatca tccagtcaaa
     1261 acttattcgc gccgctctgg tgtgcgctgc taaaaacgac gttcgttatt acctgaacgg
     1321 tcttcacatc acgccgaaac atattgaggc aaccaatggt tccgtggcac tgcgcatggc
     1381 tcacggcatc cggacgaaga aaaacatcat tgtccagttc gaagggggcg tcccggccaa
     1441 agccgaaacg acagagctga tttttagtaa agagccgatc gctgttcatc gcgaccagtt
     1501 tcagcgacga ctgtccatta ccggcattaa actggtggac ggttgttttc cggatttgga
     1561 tcgcatcatt ccgaaaaagt ttgaccgctg tactcacccg gtgctccagg cgggttacct
     1621 gagttatcca gagaagatgt ttggtcgtga gcgtaaattt attcccgtcc agttacgtcc
     1681 ctccggtgac gggcaagcgg tcagaattca gtttgattcc atcatcaact caatgtatgg
     1741 caatcctgaa tttgttgtga tgccttgtcg tgatcatggc gatttcaacg tggctcagga
     1801 gcatccggaa tgaaaatcga ataccaggac gccgcaggag gtgaatcaat gagctggcct
     1861 gatgcaatcg taactctggg gatggtattc gcagtagcgt ttgttgtgta ctcgatttgt
     1921 cgatggggat aaccacatgt tcgctttgat tcaacgcggg cagatataca cagacagagc
     1981 cggataccca gtagaaatta ctcgcagtac tgagcactca gtgttctttc ggaggatgga
     2041 cggacgtacc gggcgagtac gcatcggtga attcagtagc ctggtcgagc atattgacca
     2101 cctggaatat cacaagattc tggctgaaac agaacaagaa aagcacctga agaaattacg
     2161 cgcaatgcaa aggaagtaaa aaatgaataa agcgtttgag ctatgggtcc accagcgtta
     2221 cggcaaccac tacgacctga cgcgagatgt tgacggtttc tactgtcgtg aaattgtgaa
     2281 acgaatgttt gaggactggt gccactgccg tgggctgagt gttgtgtgag gtaatgcatg
     2341 ggcaatgtga ttcaactggc tcccaatgaa tgggtttgtg aaagcgttct aatcgcaatt
     2401 accgggctca aaccaggcac aattcttcgg acccggaaag aatgctggat ggttggaaga
     2461 gagtatattc acgtatcacc agacggtaat ccaaagc
//
LOCUS       DAJVPP010000217         2430 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVPP010000217 DAJVPP010000000
VERSION     DAJVPP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.224"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(64..822)
                     /locus_tag="OQM34_004064"
     CDS             complement(64..822)
                     /locus_tag="OQM34_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1454272.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OQM34_004065"
     CDS             complement(830..1933)
                     /locus_tag="OQM34_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1454273.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2430)
                     /locus_tag="OQM34_004066"
     CDS             complement(1943..>2430)
                     /locus_tag="OQM34_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1454274.1"
                     /translation="WLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LATAIGYPDMVSLFAGTVLNQTGQAIETIAMIMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
BASE COUNT          665 a          649 c          565 g          551 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatgatc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggtggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt
//
LOCUS       DAJVPP010000218         2414 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVPP010000218 DAJVPP010000000
VERSION     DAJVPP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.225"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1022
                     /gene="btsS"
                     /locus_tag="OQM34_004067"
     CDS             <1..1022
                     /gene="btsS"
                     /locus_tag="OQM34_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1454275.1"
                     /translation="LRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDH
                     HLPGKPISSTYTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMG
                     TIKLYEAKNRLFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPH
                     FLFNALNTIKAVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIE
                     KARFQSRLQVNIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQ
                     HLMLEIEDNAGLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLP
                     WRDEA"
     gene            1019..1738
                     /gene="yehT"
                     /locus_tag="OQM34_004068"
     CDS             1019..1738
                     /gene="yehT"
                     /locus_tag="OQM34_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS1454276.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            1785..2255
                     /locus_tag="OQM34_004069"
     CDS             1785..2255
                     /locus_tag="OQM34_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS1454277.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(2296..>2414)
                     /locus_tag="OQM34_004070"
     CDS             complement(2296..>2414)
                     /locus_tag="OQM34_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCS1454278.1"
                     /translation="KALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
BASE COUNT          630 a          557 c          632 g          595 t
ORIGIN      
        1 ttttgcgaca ggggtttaac gaagtgaaca gcatgaaagt ggctcaggta ctgtatcagg
       61 agctggatat tggtgcagtc gcgattaccg atcgagagaa attgctggcc tttaccggaa
      121 ttggtgacga ccaccattta cccggcaaac cgatttcttc gacttacacc ttaaaagcga
      181 ttgaaaccgg tgaagtggtc tacgctgatg gcaacgaagt accttatcgt tgctctttgc
      241 atccgcaatg caaactgggg tcgacgctgg taattccgtt gcgtggcgaa aatcagcgcg
      301 tgatgggcac catcaaattg tatgaagcca aaaaccggtt attcagttca ataaaccgca
      361 cgctgggcga ggggattgcg caactgcttt cggcgcagat tcttgccggg caatatgagc
      421 ggcaaaaagc gatgctcacc cagtcagaaa tcaaactgct tcacgcccag gtgaatcctc
      481 attttttgtt taatgcgctt aacaccatta aagcggtgat ccgccgcgac agcgaacagg
      541 ccagccagct ggtgcagtat ctttccactt ttttccgcaa aaacttaaag cggccttcgg
      601 agtttgttac tctcgccgac gaaattgaac atgtgaacgc ttatctgcaa attgaaaagg
      661 cgcgcttcca gtcgcggttg caggtcaaca ttgctattcc gcaagaatta tcccagcagc
      721 aattgcccgc gtttaccctg caaccgatag tggaaaacgc cattaaacat gggacatcac
      781 aactgctgga tacagggcga gtggcaatca gcgcccgacg tgaggggcaa catttgatgc
      841 tggagatcga agacaatgcc ggtttgtatc aaccggtaac caatgccagt gggctgggga
      901 tgaatctggt ggataagcgt ttacgtgaac ggtttggcga tgactatgga ataagcgtcg
      961 cctgtgagcc tgatagttac acccgaataa cgttacgact accatggagg gacgaggcat
     1021 gattaaagtc ttaattgtcg atgatgaacc gttagcacgg gagaacctgc gtatattttt
     1081 gcaggagcag agcgatattg aaatcgttgg agagtgttca aacgccgtgg aagggatcgg
     1141 cgcggtgcat aaactgcgcc cggatgtgct gtttctcgat atccagatgc cgcgcatcag
     1201 tggtctggaa atggtgggga tgcttgaccc ggaacatcgc ccgtatattg tttttctcac
     1261 tgcgtttgac gaatacgcta ttaaagcctt tgaagaacat gcctttgatt atctgctgaa
     1321 gccaattgat gaagcgcgac tggagaaaac gctggcgcga ttgcgtcagg agcgcagcaa
     1381 gcaggatgtt tcgctgttac cggaaaatca acaggcgctg aaatttatcc cttgtacggg
     1441 gcatagtcgg atttatttgc tgcaaatgaa agatgtggca tttgtcagca gtcggatgag
     1501 cggtgtctac gttaccagcc acgaagggaa agagggcttt accgaattga cattacgtac
     1561 cttggaaagt cgtacaccac tactgcgctg ccatcgtcag tatctggtta acctcgcgca
     1621 tttacaggag attcgtctgg aagataacgg ccaggccgag ttgattttgc gtaatggctt
     1681 aaccgtgccg gtcagccgcc gttatctgaa aagcttaaaa gaggcgattg gcctgtaaaa
     1741 gactgctaaa atggcttttt gcctgatcaa cacctgaagg cctcatgata agtaacgata
     1801 ttctgcgcag cgtgcgctac attttgaaag ccaataataa tgacctggtg cgtattctgg
     1861 cgctggataa tgtcgaagcc accgcggaac agatcgccgt ctggctacgt aaagaagacg
     1921 aagagggttt tcagcgttgt ccggacattg ttttgtcgtc attcctcaat ggcctgattt
     1981 atgaaaaacg cggcaaggat gagtctgctc cggcactgga gccggaacgt cgcattaata
     2041 acaacatcgt gctgaaaaaa ttacgcatcg cgttttcgct gaaaaccgat gacattctgg
     2101 ctatcctcac cgaacagcag ttccgcgttt cgatgccgga aattacagcg atgatgcgcg
     2161 caccggatca taaaaacttc cgcgaatgcg gcgatcaatt tttacgttat tttctgcgtg
     2221 gactggcagc gcgccagcat gtgaagaaaa gctaagacgg gtatggcggc catgcgaaac
     2281 atggccgccg acagattatt tcacttcttt aaaaccagcg gctttcatca ccagttccat
     2341 ttgcgccata gtgatacctt ttttggcatc ttcagcagaa acgttgattc ctgaaatacc
     2401 ctgcagggct ttaa
//
LOCUS       DAJVPP010000219         2263 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVPP010000219 DAJVPP010000000
VERSION     DAJVPP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.226"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..936)
                     /locus_tag="OQM34_004071"
                     /pseudo
     CDS             complement(1..936)
                     /locus_tag="OQM34_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(1250..>2263)
                     /gene="hemW"
                     /locus_tag="OQM34_004072"
     CDS             complement(1250..>2263)
                     /gene="hemW"
                     /locus_tag="OQM34_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1454279.1"
                     /translation="HLLCDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVR
                     ARLPLAADAEITMEANPGTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQ
                     EAKRAAKLASGLGLRSFNLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPN
                     TLFGSRPPVLPDDDALWDIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGD
                     YIGIGCGAHGKVTFPDGRILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFR
                     LLEAAPRVEFIAYTGLCEDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELF
                     LAE"
BASE COUNT          604 a          536 c          488 g          635 t
ORIGIN      
        1 aacaaagcag cttgttttgg atcttttttg aagtcgtcaa acagaggttg cacggcttta
       61 cggaatggcg ctttatccac ttttacaatt tcacccccca tagctttcgc ctgagcacgt
      121 gtgtcagcgt caattttttc ccacaatttt tgttgataag cttcggattt cttcgccgct
      181 gtttcgagga tttgttgctg ttctggggtt aatttattcc atgttttggt ggagataacc
      241 aaaaagtcag gaattgaggc gtgttcatct tcagaaaaaa ctttggcaat ttcaatatgt
      301 cgagtctgca tccaggaagg cacgttattt tctgcgccat ctactacacc ttgctgcatc
      361 gcagtataaa cttcgccaaa tgaaattgga gtgggtgaac cacccatcag ttcaatcatt
      421 ttgatggtgg tcggacttgg ctgaacacga attttcagtc ctttaagatc ttctggcttt
      481 gtaatcggtt ttttcgcata gaaactgcga ttacccgcta cataagctga taaagcaaaa
      541 aagcctttgt cttttgttga gtccataatt tctttaccaa cttcaccaaa aacaacttaa
      601 taagaacggc aaattataga tagcataaat attatcaaaa gactccagat cactggcgga
      661 acctttggtc atatccagtg cgccattttg caacaattcc atagtttctc gcgcattacc
      721 catctggctg ctgggataaa tacggatcac catattacct tttgacagct gttttacctc
      781 tttcgccagc tcttcaaaac tctgatgtac aacatgactt ctttccaggt tgtgtgctaa
      841 ctttaacgcc actttttctg cggcgtttac gccagtgcta accagagcca gcaaggatgc
      901 gcagactaat gagcgggtaa atgacctcat tttcataaaa aaactccctg ttgaaggatg
      961 gaaattggtt gatatctaat tgtgcttatc agtgatttgt gcgacaaatt ggttaatctg
     1021 tctgacaact tgtctatgtt tatcacaact tgaaataaag tcagaaatta aatattaaat
     1081 ttgtgcgcca ggttgtacat atttataatc aatgaatgat tagtaaatat tcactaaata
     1141 tatataataa atgtcgtcga tatttattca gtaagaaata tatttctgcc gaggttaatt
     1201 aacgggggga taaaagaagt gattaatgcg cccgcaggcg cattaatgat tactcagcga
     1261 ggaataattc aagaagtgag tttaagaata atttaccatg ttccgttatc tgccagtaat
     1321 ccgcacattc ggtgagataa ccctgggcaa tcgcctcgtc taactgtggg cgaatcacat
     1381 cttcgcaaag cccggtatac gcaataaact ccacgcgcgg cgcggcctcc agcagacgga
     1441 agcgattcat aaagaactca aacggcttat ctgcggcttc gacatcacgc tggctttcca
     1501 gataccttcc ttgcataaaa ccacgaggat gacgcgtttt agtggtacgc agaatgcgcc
     1561 catccgggaa ggtcactttg ccgtgcgcgc cgcagccaat accgatgtag tcaccaaagc
     1621 gccagtagtt gagattgtgc tggcactgat aaccgggttt ggcgtaagcg gaagtttcat
     1681 attgctgata acccgctgcg gttaataact gatgcccctg ttcgaaaata tcccacagcg
     1741 cgtcatcgtc cggcagcacc ggcggtcgcg agccaaacag cgtattaggt tcgatagtca
     1801 gttgatacca ggaaagatgc ggcggattca gttcaatggc ctggcgtaga tcgccaagcg
     1861 cctcttccag tgattgatcc ggtagtccat gcatcaaatc aaggttaaag ctacgtaacc
     1921 ctaaaccgct cgccagcttt gccgctcgtt tcgcttcttg cgggccatga atacgcccaa
     1981 gtcgtttcag cttttcttcg ctaaaactct gcacaccaat agagatgcgg tttacaccag
     2041 cacgctgata atcgacaaag cgatccgctt ctaccgtgcc tgggttcgct tccatagtaa
     2101 tttctgcatc cgctgccagc ggcaaacgcg cacgcacgcc gtctagcagc gtttgcatcg
     2161 ccgggccgga aagcaggctc ggcgtaccac cgccaataaa gattgtcttt acttcacggc
     2221 cctgagcgta agccacatca ttgtccagat cgcaaagcag atg
//
LOCUS       DAJVPP010000220         2142 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVPP010000220 DAJVPP010000000
VERSION     DAJVPP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.227"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>2142
                     /locus_tag="OQM34_004073"
     rRNA            <1..>2142
                     /locus_tag="OQM34_004073"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          587 a          468 c          657 g          430 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgtttgatgt tc
//
LOCUS       DAJVPP010000221         2141 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVPP010000221 DAJVPP010000000
VERSION     DAJVPP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.228"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..757)
                     /gene="ipaH7.8"
                     /locus_tag="OQM34_004074"
     CDS             complement(<1..757)
                     /gene="ipaH7.8"
                     /locus_tag="OQM34_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCS1454280.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPD"
     gene            1086..1265
                     /locus_tag="OQM34_004075"
                     /pseudo
     CDS             1086..1265
                     /locus_tag="OQM34_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1282..1972)
                     /locus_tag="OQM34_004076"
                     /pseudo
     CDS             complement(1282..1972)
                     /locus_tag="OQM34_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          633 a          393 c          484 g          631 t
ORIGIN      
        1 agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac aatggattcc
       61 ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga agagtggtta
      121 tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca gaaaattgag
      181 gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc ggtaagtcgg
      241 gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg ggaagagccg
      301 gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt ggcaaaacag
      361 gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca ggaagttctg
      421 gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca ggcagctttg
      481 gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt ggtaattcgg
      541 gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc tgaaagcact
      601 gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc ttttcccatt
      661 cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata gagggggagc
      721 aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa ccttatattt
      781 gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat ttccgatatt
      841 aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta tttatggagg
      901 ttttctatgg aattcttttt ttattgtgac tgcagtcaat aataacactg aattcaacat
      961 tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca tattttagtc
     1021 aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa aacgatacac
     1081 atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag aaatgtgtgc
     1141 cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg tcaaaccatg
     1201 ctgaaatggt aaaggcgttc aactatatcc tgaattactg gaatgcactg aatgagtttt
     1261 gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac cgatgacttt
     1321 ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc gcttgatgcg
     1381 ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc ggtcaggtgt
     1441 ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac catgccgata
     1501 cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg tggcccgcaa
     1561 gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca gtgttgttgc
     1621 gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc aacatcagcg
     1681 tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc cgtatgacgg
     1741 tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg ttatgtgcca
     1801 tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa gtgagctgga
     1861 aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta cggccatgtc
     1921 gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc atggaaaacc
     1981 tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct tttgccgcaa
     2041 taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa aaaaattatc
     2101 ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DAJVPP010000222         2105 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVPP010000222 DAJVPP010000000
VERSION     DAJVPP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.229"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..383)
                     /locus_tag="OQM34_004077"
                     /pseudo
     CDS             complement(1..383)
                     /locus_tag="OQM34_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(380..1132)
                     /gene="bcsQ"
                     /locus_tag="OQM34_004078"
     CDS             complement(380..1132)
                     /gene="bcsQ"
                     /locus_tag="OQM34_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1454281.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(1144..1332)
                     /locus_tag="OQM34_004079"
     CDS             complement(1144..1332)
                     /locus_tag="OQM34_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1454282.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            1605..>2105
                     /locus_tag="OQM34_004080"
     CDS             1605..>2105
                     /locus_tag="OQM34_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1454283.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRS"
BASE COUNT          549 a          524 c          497 g          535 t
ORIGIN      
        1 gctaacgcgc tcaggcagct cgttcatcca ttgatggtaa cgtccacgaa tattttgcag
       61 acctgaaaat gcccgctgcg cggtttcggc gtttctttgc gcgatgcacc gatcaataac
      121 cagcatgttt gaatgagata acggaccggg tccagcggac gcggacgcga ggcgttgata
      181 tgcgtataca ggtttttatg ttctgcgcga atacgctgcc agcgcgggtg ctccagcgga
      241 ataaaaatcc aggccaggat catccagaaa cagccgagcg tcgcgctgaa agccgacgca
      301 ccgtgacgac gataatcgcg ataacgcccg ataagccgcg cgttgaccgg cgggataagc
      361 aaccaccggg tcaggatact catgatgcac tcccgactgg cgttttcagc ccggagtagt
      421 tcaacaggca ccagttcgcc agcgtcagta tctcttcagc cgccagcgca tcactgcgat
      481 attcacctac tggttgctta gccgccaggc attcagccat cgcttcatca cgatgaatga
      541 gcatcggcag taatcggcgc tggctttgca accaaagctg gtaaatatcg tcctgaacct
      601 gactgccaat acggaagtca ttaatcaaaa tatgtgcgcc atccggcagc gcttgctgat
      661 gcagtcggat atggcagttg gcatccacat tgacgattgc cagcgagtgc tcgcacaaac
      721 tcagcagctg gtgggttatc tgcgaggcat cacgcggtaa gtcgattaaa atccactggt
      781 aacgcccgct ggcttttagt tgctgtaagc cggagcaaat atcgctcagc cgggtttgcc
      841 agtgctgtgg attttcttgt tcttcaatgg ataactgacc aaaaggcagc aaatcgagct
      901 gcgaggtgta gcgcaaccca gcgtcacgcc agtcctggcc atccagcatc gctctggccc
      961 agccctgacg gtgggtaaaa tcaacattaa atgacaggcg caacaagttg tccgggcagg
     1021 catcgaccac caggacattt tctcccaaca tttgtaatga ccaggctaat gcggcggtga
     1081 tggttgttgt ccccacgcct ccccgcaccc cctgcaatcc cagtacggcc attcaccaat
     1141 cccctacttt tgttgcgcaa actctgccag caacggccag cgttttaatg ccgcggccaa
     1201 ctgttcgcgt tgggaaatat cggcataatc aatatcaggc agtgaaaatg cctgctttaa
     1261 cgccacaata tcattctgga agatatagcc tatcgcggga tcaggcagag tatctggttc
     1321 attgttattc attttgcttg atccctatga aataaccgta ccattcatga gacaattcta
     1381 accgtgagca tttcgccggt ttattgttta cggcgagacg ggaatttgct cactttttga
     1441 ctttccgtta cgcaacagaa aaagttaaaa acgaaaagac taaaatcaca cgtcttgtga
     1501 ccttctaaat tgattcacat gctaaatctg ataagtttta atttcaatgg taggtttatt
     1561 tcttagcttt cgctagtaaa ctgataatca gacaaaatgg tgacatgagg gacattgtgg
     1621 accctgtatt ctctatcggt atctcatcat tatgggatga gctgcgacat atgccagcag
     1681 gcggcgtctg gtggtttaac gtcgatcgcc atgaagatgc tatcagtctg gcgaatcaaa
     1741 caattgcatc ccaggctgaa accgcacacg tcgcggtcat tagcatggac agcgatccgg
     1801 cgaaaatctt tcaattagat gattctcaag ggccggaaaa aataaaatta ttttcaatgc
     1861 taaatcatga aaaaggtcta tactatttgg cccgtgattt gcagtgttct attgatcccc
     1921 ataattacct ttttattctt gtttgcgcaa ataacgcatg gcaaaatatt cctgccgagc
     1981 ggcttcgctc atggttggat aaaatgaata aatggagcag gttaaaccat tgttcgcttt
     2041 tggtaattaa tcccggaaat aataacgata aacaattttc attgttgctt gaggaatacc
     2101 gttca
//
LOCUS       DAJVPP010000223         1994 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVPP010000223 DAJVPP010000000
VERSION     DAJVPP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.230"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..538
                     /locus_tag="OQM34_004081"
     CDS             <1..538
                     /locus_tag="OQM34_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCS1454284.1"
                     /translation="QAADNNMEFANYGPFELLQARQLIESNIAEFAATQVTKQDIMKL
                     MAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALAAIVEKMWTQRSHNPYWKKLHEH
                     IDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHLENTKIMLFNETSDDFEFNADRY
                     LFAENPVVHLDTATSGSK"
     gene            883..1545
                     /locus_tag="OQM34_004082"
     CDS             883..1545
                     /locus_tag="OQM34_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1454285.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1549..1932
                     /gene="mzrA"
                     /locus_tag="OQM34_004083"
     CDS             1549..1932
                     /gene="mzrA"
                     /locus_tag="OQM34_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1454286.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
BASE COUNT          491 a          503 c          478 g          522 t
ORIGIN      
        1 gcaggcggct gacaataata tggaattcgc caattacggt ccgtttgagt tgcttcaggc
       61 tcgccagctc atcgaaagta atattgccga gttcgcggca actcaggtaa cgaaacaaga
      121 catcatgaaa ctgatggcca ttcaggaaca ggcgcgcggc gaacaatgct tccgtgattc
      181 cgagtgggat ttacagttcc acattcaggt agccctggcg acgcagaact ccgccctggc
      241 ggccatcgtt gaaaaaatgt ggacccagcg tagccataac ccgtactgga aaaaactaca
      301 cgaacacatt gattcccgta ccgttgataa ctggtgtgat gaccacgatc aaattctcaa
      361 ggcgttaatt cgcaaagatc ctcatgctgc taagctggca atgtggcagc atctggaaaa
      421 caccaaaatc atgttattta acgaaaccag cgacgatttc gagttcaatg ccgaccgcta
      481 tctgttcgcc gaaaacccgg tagtgcatct cgatacggcc accagcggca gcaaatgaaa
      541 ttcctgtccg acaggcgctg caatgcgcct gtttgtctcg tcggtaagcg aaaagtataa
      601 agtgtcagcc tgtgtaaatc ctctcgccac cctcccctgc aatcagcaaa atcagactcc
      661 acggacatgt aattttgata acgaacaacg ttgacctttg ttacaattag attcaatttg
      721 aatttatgct tttgaatgct ttcttatctc acgttttaac agggaatagt tcaggctgtg
      781 ttgatgtatc aaacccgcag aacataccaa aacagcaata acattgcggt agtgcatctt
      841 ttaaaaccag cgtggcgtta accgattcac caggaataat gaatggaact tttgacccaa
      901 ttgctgcaag ccctgtgggc gcaggatttt gaaaccctgg ccaatccatc gatgattggc
      961 atgttgtatt ttgtcttgtt tgtaattttg ttccttgaaa atggcttgct tccggcggcc
     1021 tttttaccgg gcgacagttt actggtattg gtcggcgtgt tgattgcgaa aggcgcgatg
     1081 ggttacccgc aaacgattct gctgctgacc gttgccgcca gcctcggttg ctgggtcagc
     1141 tatattcagg ggcgatggct gggcaatacc cgcaccgtac aaaactggct atctcattta
     1201 cccgcgcatt atcatcaacg cgcacaccat ctttttcata aacacggttt atcggcgctg
     1261 ttaattggtc gctttattgc atttgtcaga acactgctgc cgacgattgc cgggttatca
     1321 gggctgaata acgcgcgctt tcagttcttc aactggatga gcggtctgct gtgggtattg
     1381 atcctgacaa ctctgggtta catgctcggc aaaacgccgg tatttttaaa gtacgaggac
     1441 cagctgatgt catgcctgat gctgctcccg gtggtgctgc tggtgtttgg cctggcaggt
     1501 tctctggtcg tgttatggaa aaagaaatat ggaaatcggg ggtaagggat gcaaatacct
     1561 cgcatgtcgc ttcgccagct agcctggtcc ggcactgttt tacttctggt cggcacgctg
     1621 ttgctggcct ggtccgcggt tcgccagcaa gagtctacgc tggcgattcg tgccgttcat
     1681 caaggcacaa cgatgccaga cggtttttcg atctggcatc accttgacgc tcatggcatt
     1741 cctttcaaaa gtatcacccc caaaaacgac actctgttaa ttacatttga ctccagcgac
     1801 cagagcgccg ccgcaaaagc ggtcctcgac agaacattgc cccatggcta catcattgcg
     1861 cagcaggaca ataacagtca ggctatgcag tggctgaccc ggttacggga taactctcat
     1921 cgcttcggat aacttcctgt tatccgaaac atatcactca ctttggtgat ttcaccgtaa
     1981 ctgtctatga ttaa
//
LOCUS       DAJVPP010000224         1968 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVPP010000224 DAJVPP010000000
VERSION     DAJVPP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.231"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..155
                     /locus_tag="OQM34_004084"
     CDS             <1..155
                     /locus_tag="OQM34_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCS1454287.1"
                     /translation="YWFLQWLDQVRPSQWAAIGMLGSLLLGLLTYLTNLYFKIREDKR
                     KAARGE"
     gene            159..788
                     /locus_tag="OQM34_004085"
     CDS             159..788
                     /locus_tag="OQM34_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454288.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            854..1387
                     /locus_tag="OQM34_004086"
     CDS             854..1387
                     /locus_tag="OQM34_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1454289.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            1384..1851
                     /locus_tag="OQM34_004087"
     CDS             1384..1851
                     /locus_tag="OQM34_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1454290.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            1839..>1968
                     /locus_tag="OQM34_004088"
     CDS             1839..>1968
                     /locus_tag="OQM34_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1454291.1"
                     /translation="MPSMIEIILLIILHIWLCRQGGDHFWSESRLNISLLMLKVEHL"
BASE COUNT          528 a          432 c          529 g          479 t
ORIGIN      
        1 gctactggtt tttgcagtgg ttggatcagg tcaggccatc acagtgggct gcgattggta
       61 tgctgggaag tctgcttctg gggcttctga cttatctgac gaatctgtat ttcaaaataa
      121 gagaagacaa gcgtaaggct gcacggggag agtaaataat gaagcatgaa gaaatgaatc
      181 agcgattcaa tcacctggaa aatgaaatca ctgagcttaa taaaaaattg tcggcgttgg
      241 tgagttctga agatgaaaac aaacgccgcg atgagcatta tgcggcgatt tacgattact
      301 gccacaaagt cgctcacgag acttttatga agtttttgca ggaaaagttt ttaccggccg
      361 cattgtcaga aaaagaggcg gcttacctgc ggcctgagta cgtcattacg gttaacagtg
      421 ccggagagga agagcataaa agtgatttta ttgcgtctgc accggataaa gaccaggaac
      481 cccatcggcc tttcagggtt tcctgtgaag agggtgagtt cgtcgtttat gagaacggaa
      541 aacctgtccg ggcatcgcat catcactgcc tgaaaattat taatctcgct atcagatgcc
      601 tgaaagacga aaacaccaga gttatgaaac gcattggccg ttgcatgggg tatttgcagg
      661 tggcggcaga gattgaagca cttgccagtg gtgcagacat ggatgctgcg gtgcgggagg
      721 ctcttcttcg tgatttcaat actccccctt taagaaagag cctgatgact ggatccagcc
      781 ggggctgact tatcttaaaa ggcgtatata agttggctcg ttatttgttg ccgataaatc
      841 ctgataaata tccatgagcg caaaaatcaa atacggcctg tcggctgctg ttctggcgct
      901 gattgccgca ggcgcgtctg ctccccagat acttgaccag tttctgaacg aaaaagaggg
      961 taatcacaca acggcatacc gtgatggttc tggcatatgg accatctgtc gtggtgccac
     1021 aatggtggat ggtaagcctg tcataccggg aatgaagctg tcgaaggaaa aatgtgacca
     1081 ggttaacgct attgaacgtg ataaggcgct ggcatgggtg gagcgcaata ttaaagtacc
     1141 tctgaccgaa ccccagaaag cgggtatagc gtcattttgc ccctataaca ttggccccgg
     1201 taagtgtttc ccgtcgacgt tttataagcg gctgaatgcc ggtgatcgta agggcgcatg
     1261 cgaggcgatt cgctggtgga taaaagatgt tgggcgcgat tgccgcatac gttcaaataa
     1321 ctgctatgga caggttattc gtcgtgacca ggaaagcgca ttagcctgtt gggggataga
     1381 tcaatgagca gagtcaccgc gattatctcc gctctggtta tctgcatcat cgtctgcctg
     1441 tcatgggctg ttaatcatta ccgtgataac gccattacct acaaagagca gcgcgacaaa
     1501 aacgccggag aactgaagct ggcgaacgca accattactg acatgcagca gcgccagcgt
     1561 gatgctgatg cactcgatgc taaatacacg aaggagttag ctgatgcgaa agctgaaaat
     1621 gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg aaaatgccct
     1681 gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc caccgtcgaa
     1741 ctctctccag ttgctggacg aaacgttctc ggtatccggg acggaatcat cagagaccag
     1801 acagcactga gaacgcttca ggaatatatc aggacgcaat gccttcgatg atagagataa
     1861 ttttactcat catccttcac atctggctct gtagacaggg tggtgatcac ttctggagtg
     1921 aatccagatt aaacatctca ttgctgatgc ttaaagttga gcatttgg
//
LOCUS       DAJVPP010000225         1924 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVPP010000225 DAJVPP010000000
VERSION     DAJVPP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.232"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            98..1129
                     /gene="mdtN"
                     /locus_tag="OQM34_004089"
     CDS             98..1129
                     /gene="mdtN"
                     /locus_tag="OQM34_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1454292.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1129..>1924
                     /gene="mdtO"
                     /locus_tag="OQM34_004090"
     CDS             1129..>1924
                     /gene="mdtO"
                     /locus_tag="OQM34_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1454293.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQA"
BASE COUNT          400 a          533 c          519 g          472 t
ORIGIN      
        1 caatctggca tttgtcggaa ttatttatac cgcccttttt gctctctacg ccatgctgtt
       61 ctggctggca tttttctaat ctatttgaga cgcgctcatg gaaagtacgc cgaaaaaagc
      121 acctcgcagt aaattccctg ctctgttagt ggttgcgttg gcgctggttg cccttgtttt
      181 cgttatctgg cgcgtagaca gtgcgccatc aactaatgac gcttacgcgt cagcagatac
      241 cattgatgtg gtgccggaag tcagcggtcg cattgtagaa ctggcggtca ccgacaacca
      301 ggcggtgaaa cagggcgatt tgctgttccg catcgacccg cgcccgtacg aagccaacct
      361 ggcgaaagcc gaagcctccc tcgcggcgct ggataagcaa attatgctca cccagcgtag
      421 cgttgacgcc caacagtttg gtgccgactc ggttaatgcc acggtagaaa aagcccgtgc
      481 cgccgcgaaa caggccacag atacattacg ccgcaccgag ccattactga aagagggttt
      541 tgtctcagca gaagacgttg accgtgcaag aacggcgcag cgcgccgcag aagcggatct
      601 taatgccgta ttgttacagg cgcagtcagc cgccagcgcc gtcagcggcg tcgatgcgct
      661 ggtggcgcaa cgtgcggcgg tcgaagcgga tattgccctg accaaactac atctggaaat
      721 gacgaccgtt cgcgcgccat ttgatggccg gatcatttcc ctcaaaacct ccgtcgggca
      781 atttgcttct gccatgcgcc ctatttttac cctcatcgac actcgtcact ggtacgtgat
      841 cgccaacttc cgcgaaaccg atctgaaaaa tattcgctca ggtacacccg caacgattcg
      901 cctgatgagt gacagcggca aaaccttcga gggtaaagtg gattcgattg gctacggcgt
      961 gctaccggat gacggcggcc tggtgctggg cggcctgcca aaagtgtctc gttctattaa
     1021 ctgggtccgc gttgcccagc gttttccggt caaaatcatg gtcgataaac ctgacccgga
     1081 aatgttccgc atcggcgctt cggcagtcgc taatcttgag ccgcaataat gagcgcgctc
     1141 aactccctgc cattaccggt ggtcaggctg ctggcgttct ttcatgaaga gttaagcgag
     1201 cggcgacccg gccgcgtgcc gcagatcgtg caactctggg taggctgcct gctggtgatt
     1261 ctgatctcga tgacttttga gatccctttt gtggcgttat cgctggcagt gctgttttac
     1321 ggtattcagt cgaacgcgtt ttacaccaaa tttgtggcga tcttgtttgt ggttgccacg
     1381 gtgctggaga tcggcagcct gtttttgatc tacaaatggt catacggcga accgttgatc
     1441 cgattgatca tcgccgggcc gatcctgatg ggctgcatgt ttttgatgcg cacccatcgg
     1501 ctgggactgg tctttttcgc tgtcgccatt tacgggcaaa ccttccccgc catgctcgac
     1561 tatccggaag tagtcgtgcg cttaacgctg tggtgtatcg ttgttggcct ctatccaacc
     1621 ttgctgatga cgttaatcgg cgtgctgtgg tttcccaatc gtgccattac gcaaatgcat
     1681 caagcgctta atgatcggct tgatgatgcc attagccacc tgacggacag cctcgcaccg
     1741 ctacccgaaa cgcggattga aagagaggcg ctggcgctgc aaaaactgaa tgtcttttgt
     1801 ctcgcggacg atgccaactg gcgaactcaa agcgcatggt ggcaaagctg cgtggcaacg
     1861 gtaacctaca tttactcgac gctgaatcgc tacgatccca cctcttttgc tgattctcag
     1921 gcaa
//
LOCUS       DAJVPP010000226         1896 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVPP010000226 DAJVPP010000000
VERSION     DAJVPP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.233"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(210..374)
                     /locus_tag="OQM34_004091"
     CDS             complement(210..374)
                     /locus_tag="OQM34_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454294.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YARNAEIPGL"
     gene            1276..1560
                     /locus_tag="OQM34_004092"
     CDS             1276..1560
                     /locus_tag="OQM34_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1454295.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1548..>1896
                     /locus_tag="OQM34_004093"
     CDS             1548..>1896
                     /locus_tag="OQM34_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1454296.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCY"
BASE COUNT          481 a          399 c          556 g          460 t
ORIGIN      
        1 ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga gccggttact
       61 ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt ctgcaatacc
      121 ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata gctgatggag
      181 ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc gtttctggcg
      241 taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt atgagagatg
      301 aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt aagagcggtg
      361 gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt gcggagatga
      421 acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc gaaaatgtgc
      481 tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc ggcatccggc
      541 atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc tgtcgtctcg
      601 atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg atgcgagaca
      661 caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg agcgaagggg
      721 gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc catgagtcag
      781 ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg ggtcaaagct
      841 aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt taccaatcga
      901 agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt gcccgcaaac
      961 acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga attcgtcacg
     1021 actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg gttcaacgac
     1081 aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg ggggcggtgc
     1141 ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta aatgccgctg
     1201 acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg tcaatatgct
     1261 attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca gctcaatctc
     1321 agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct tcataagtcg
     1381 cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat tctcgaccgg
     1441 cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct tgatgctccg
     1501 gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg ggatgtgtaa
     1561 ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc agtgaagctg
     1621 tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga gccacccgga
     1681 cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca ctggccactg
     1741 gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca gaccccattc
     1801 ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa gggcttggcg
     1861 cggatttact tcatgatgca gttcgtcgtt gttaca
//
LOCUS       DAJVPP010000227         1881 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVPP010000227 DAJVPP010000000
VERSION     DAJVPP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.234"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            362..505
                     /locus_tag="OQM34_004094"
                     /pseudo
     CDS             362..505
                     /locus_tag="OQM34_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            653..919
                     /gene="ospE1"
                     /locus_tag="OQM34_004095"
     CDS             653..919
                     /gene="ospE1"
                     /locus_tag="OQM34_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCS1454297.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1309..>1881
                     /locus_tag="OQM34_004096"
     CDS             1309..>1881
                     /locus_tag="OQM34_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1454298.1"
                     /translation="MIKSTNIQAIGSRIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPD"
BASE COUNT          635 a          398 c          313 g          535 t
ORIGIN      
        1 ttgaaataaa gctcaatagc gcgaattttt tgttcttcag taaacatgtt tcatcttctg
       61 agagtccagg aaatcgtccg catccccatt accgtcttat atcactggcg ctgacatgtg
      121 ctcattgatg aagtgaatct gaatgacatg ttttgccata tcaacgccaa ccggtgtata
      181 tttcatttgt ggagtctcca gtctgaggac gctttatgca tcccgtattg ggcagtatga
      241 tgccggaaag ctgcgaggct ccacacctgc cagtttcacc cttttaacca tcatactact
      301 ccgtgtttaa ttgtgtggga tgcgttcatt acattttaac agtgtcaccc cgtcggccac
      361 gaaagataaa caggtgacca gaaaagggat cttcatccag cacatgctat atcttgaacg
      421 taacaacctg accctccgca cccgcatcaa gcggctggca cataaaacaa tctgcttctc
      481 gcgttccgtg gaaatccacg aaaagtcatc ggctccttca ttgaaaaaca catattctac
      541 tgattggaag cgccacccga aaaagtatcg tttttttaca gtaaacttca tttagccgac
      601 tataatgtaa aaaaaataaa ttttttgcca aaatataaca taaggaagta atatgcttac
      661 acaaactata ttcccttgtc ttccacaaaa acaagaaaat atcatccttg aagtatcaaa
      721 tcctgtatta ttatcttcta ctgttactac agatggatat actgttttca ataaaaaagc
      781 agctatatac gaattacaaa tacctgctgc cagccggacg aaaacgttga aattcactgc
      841 tacagaaatg caatggttga caaagataaa tgaagccgga attgatgaaa aacaaagcca
      901 acggtattct gatttctgaa gttaatttca gactcgttta agctccattt tcaaaaaaat
      961 atatcagggt ttgttactaa taacttctga aaacagaaaa aaaatagagc agtaattcta
     1021 tttcagagag gagtaactcc atctatcctc gatacgtact gaacgttatc gtgatatatt
     1081 catcatgctt tcttcaccat tattcgagta tagggagaga cgcaatgacc ttaaatacct
     1141 ttttatggct gaattaccat tctcaggaat aacagcagac tccttcccat agacggctta
     1201 gggggaaagc aatacccgga gaaagggtac aaagcaaact gttttaaaat taataagcta
     1261 tattctattt aactagaatt cttatcgttg ataagaattc tagttataat gattaaatca
     1321 accaatatac aggcaatcgg ttctagaatt atgcatcaaa taaacaatgt atactcgtta
     1381 actccattat ctttacctat ggaactgact ccatcttgta atgaatttta tttaaaaacc
     1441 tggagcgaat gggaaaagaa cggtacccca ggcgagcaac gcaatatcgc cttcaatagg
     1501 ctgaaaatat gtttacaaaa tcaagaggca gaattaaatt tatctgagct agatttaaaa
     1561 acattaccag atttaccgcc tcagataaca acactggaaa taagaaaaaa cctattaaca
     1621 catctccctg atttaccacc aatgcttaag gtaatacatg ctcaatttaa tcaactggaa
     1681 agcttacctg ccttacccga gacgttagaa gagcttaatg tgggtgataa caagataaaa
     1741 gaattaccat atcttcctga aactctaact catttacgga tccataataa ccgattgcat
     1801 attctgccac tattgccacc ggagctaaaa ttactgatag tttctggaaa cagattagac
     1861 agcatccccc cctttccaga t
//
LOCUS       DAJVPP010000228         1863 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVPP010000228 DAJVPP010000000
VERSION     DAJVPP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.235"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..657
                     /gene="agaC"
                     /locus_tag="OQM34_004097"
     CDS             <1..657
                     /gene="agaC"
                     /locus_tag="OQM34_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCS1454299.1"
                     /translation="GLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDA
                     KTAIGLGLPFSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASA
                     YAVIAFLCTYLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPY
                     LIAGFLFVCYIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI
                     "
     gene            647..1438
                     /gene="agaD"
                     /locus_tag="OQM34_004098"
     CDS             647..1438
                     /gene="agaD"
                     /locus_tag="OQM34_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCS1454300.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1439..>1863
                     /locus_tag="OQM34_004099"
     CDS             1439..>1863
                     /locus_tag="OQM34_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1454301.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKG"
BASE COUNT          422 a          458 c          493 g          490 t
ORIGIN      
        1 ggcttaatta ccggtggtct gacagagttg gctttcgccg gattaacccc tgcaggtggt
       61 gttcagccgc ccaacccgat tatggcgggt ctgatgacca ccgtcattgc atggtctacg
      121 ggcgttgatg ccaaaacagc aattggtctt ggcctgccgt ttagtttgtt aatgcagtac
      181 gtcattctgt tcttctattc cgctttctca ttatttatga ccaaagccga taaatgcgcg
      241 aaagaggcgg atacggcagc gttttcccgg cttaactgga caacgatgct catcgtcgct
      301 tcagcgtatg cggtgattgc tttcctctgt acttacctgg cacaaggggc gatgcaggcg
      361 ctggtgaaag cgatgcccgc ctggttgacc cacggctttg aagtggctgg cggtattctg
      421 cctgccgttg gttttggctt gctgctgcgc gtaatgttca aagcgcaata tatcccttac
      481 ctgatcgccg gtttcctgtt tgtttgctac atccgggtca gcaacctgtt gccggttgcc
      541 gtactgggcg caggctttgc ggtgtatgag tttttcaatg cgaaatcccg gcagcaagcg
      601 caaccgcagc ccgttgccag taaaaatgaa gaagaggact acagcaatgg gatctgaaat
      661 cagtaaaaaa gatatcaccc gtctgggctt tcgttcgtcg ctgctgcaag cgagctttaa
      721 ctacgaaagg atgcaggcgg gcggttttac ctgggcgatg ttgccgatcc tgaaaaagat
      781 ttataaggac gacaaaccgg gcttaagcgc ggcgatgaaa gataacctcg aatttattaa
      841 tacccacccg aatctggtcg gattcctgat ggggttatta atttcgatgg aagaaaaagg
      901 agaaaaccgc gacaccatta aaggcctcaa agtggcactg tttggcccaa tcgccgggat
      961 tggcgatgcg attttctggt ttactttgtt gccgattatg gcgggaattt gctcatcatt
     1021 tgccagccag ggaaacctgc tggggccgat tctatttttc gccgtttacc tgcttatctt
     1081 tttcctgcgc gtcggctgga cccacgtcgg ttattcagtc ggcgtgaagg cgatcgataa
     1141 agtgcgagag aactcgcaga tgattgctcg ctcggcaacc atcctcggga tcacggtaat
     1201 cggcgggctg atcgcttcgt atgtgcatat taacgtggtg acatcgtttg ccatcgacag
     1261 tacccacagc gtcgcgctgc aacaggattt cttcgataaa gtcttcccga acattttacc
     1321 gatggcctac accctgctga tgtattactt cctgcgggtg aaaaaagcgc atccggtgct
     1381 gttaatcggc gtgacttttg tgctctctat tgtttgttcc gcattcggca ttttgtaaat
     1441 ggaacgaggc actgcttctg gcggtgcctc tttacttaag gaatttcatc ctgtgcaaac
     1501 ccttcagcaa gttgaaaact atacggcgtt aagtgaacga gccagcgaat atttattggc
     1561 tgtgatccgt agcaaaccgg atgccgtgat ttgcctggcg accggagcca cgccattact
     1621 gacgtatcat tatctggtag aaaaaatcca ccagcagcag gttgatatca gccagctcac
     1681 cttcgtgaag ctcgacgaat gggtggatct gccattaacg atgcctggca cctgcgaaac
     1741 tttcctacaa cagcatatcg tgcagccgct ggggctacgt gaagaccagc tcatcagctt
     1801 tcgctccgaa gagataaatg agacagagtg cgaacgggta acgaacctga ttgcgcgcaa
     1861 agg
//
LOCUS       DAJVPP010000229         1856 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVPP010000229 DAJVPP010000000
VERSION     DAJVPP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.236"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..1645
                     /gene="kup"
                     /locus_tag="OQM34_004100"
     CDS             <1..1645
                     /gene="kup"
                     /locus_tag="OQM34_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS1454302.1"
                     /translation="DNAGEGGILTLMSLAGRNTSARTTSMLVIMGLIGGSFFYGEVVI
                     TPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKHGTAMVGKLFAPIMLTWF
                     LILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIALGAVVLSITGGEVLYADM
                     GHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWALIPLLIIA
                     ALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFVNWMLYVAVV
                     IVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALILIAFLCVDI
                     PLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEAMIASLEK
                     SPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPYVHNVRRVQ
                     IEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESLILGKRPW
                     YLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
BASE COUNT          388 a          445 c          502 g          521 t
ORIGIN      
        1 agataacgcc ggtgaggggg ggatcctgac gttgatgtcg cttgccgggc gtaatacgtc
       61 ggcgcgaacc acatcaatgc tggtgattat ggggctaatc ggcggcagct ttttctatgg
      121 tgaagtcgtc ataacacccg ctatttcggt gatgtcagcc attgaaggtc tggaaatcgt
      181 cgccccgcag ctggatacct ggatagttcc cctctcaatt atcgttctca ctttattatt
      241 tatgattcaa aagcatggca cggctatggt cggtaagctg tttgcgccga tcatgctgac
      301 ctggtttttg attctggcag gactggggtt acgtagcatt attgctaacc cggaagtgct
      361 gcatgcgctg aatccgatgt gggcggtgca tttcttcctt gaatacaaaa cggtttcttt
      421 tattgcatta ggggcagtgg tgctgtcgat tacggggggc gaggtgctgt atgctgatat
      481 ggggcacttt ggtaagttcc ctattcgcct ggcgtggttc accgtcgtat taccttcctt
      541 gacccttaat tacttcggtc agggagcgtt gttgttaaag aacccggaag cgattaagaa
      601 cccgttcttc ctgttggcac cggactgggc gctgatccca ctgctgatca tcgccgcact
      661 ggcgacggta attgcctcgc aggcggttat ctctggcgtc ttctcattga cgcgtcaggc
      721 ggtacgtctg ggatatttgt cgccgatgcg cattattcac acctccgaaa tggagtcagg
      781 gcaaatctat attccgtttg tgaactggat gctctatgtc gcggtcgtga ttgtgattgt
      841 cagctttgag cactccagca acctggcggc ggcgtacggg attgcggtga ccggaaccat
      901 ggtactgacg tctattctct cgactaccgt ggcacgtcag aactggcact ggaataagta
      961 ttttgttgcg ctgatcctga ttgctttcct ttgtgtcgat attccattgt tcaccgctaa
     1021 cctcgataaa ctgctctccg gcggctggtt gccattgagc ctcggtactg tgatgtttat
     1081 cgtgatgacc acctggaaga gcgagcgttt ccgcttgctg cggcggatgc atgaacatgg
     1141 taactctctg gaagcgatga ttgcttcgct ggagaaatca ccgcctgttc gcgtgcccgg
     1201 gaccgcggtg tatatgtcgc gtgcaatcaa cgtcattccc tttgcgctga tgcataacct
     1261 taaacataac aaggtattgc atgagcgggt gattctgtta actctgcgca ccgaagacgc
     1321 gccatatgtc cataacgtcc gtcgggtaca gattgaacaa ctgtcgccca ctttctggcg
     1381 cgtggtggca agttatggtt ggcgagaaac gccaaacgtg gaagaagttt tccaccgctg
     1441 cggtctggaa ggattaagtt gccggatgat ggaaacctcc ttctttatgt cgcatgagtc
     1501 gttgatcctc ggcaaacgcc cgtggtattt gcgtctgcgc ggcaagctgt acttgctgct
     1561 gcaacgtaat gcgctgcgtg caccagatca atttgaaatc ccgccaaaca gggttatcga
     1621 actgggtact caggtcgaaa tctaacgcca gacgcctcct ttcttcataa gggggcgttt
     1681 ttgttttcat ggttaatcac catgtaaaac gtttcgaggt tgatcacatt tccgtaacgt
     1741 cacgatggtt ttcccaactc agtcaggatt aagctgcgga tcagcgaaac gtttcgctga
     1801 tggagaaaaa aatgaaaaaa ggcaccgttc ttaattctga tatttcatcg gtgatc
//
LOCUS       DAJVPP010000230         1827 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVPP010000230 DAJVPP010000000
VERSION     DAJVPP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.237"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>1827
                     /gene="sat"
                     /locus_tag="OQM34_004101"
     CDS             <1..>1827
                     /gene="sat"
                     /locus_tag="OQM34_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCS1454303.1"
                     /translation="DKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIASVNGDQL
                     TWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEKITKDKDI
                     VFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESIVNWNALYS
                     SDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKVILNKDNSL
                     GNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAINNEDSYI
                     YHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGHATEHAIFR
                     STASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWKTGVFKFDT
                     LHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDFKQTISTN
                     LSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQNSMFTTK
                     GIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTGNIYADDA
                     ATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPK"
BASE COUNT          678 a          319 c          357 g          473 t
ORIGIN      
        1 atgataaaac atttcgtaat cttgaaatat tcggagacag cggctctgga gcatacttat
       61 atgataacaa actagaaaaa tgggtattag tcggaacaac ccatggtatt gccagcgtta
      121 atggtgacca actgacatgg ataacaaaat acaatgataa actggttagt gagttaaaag
      181 atacctatag tcataaaata aatctgaatg gcaataatgt aaccattaaa aacacagata
      241 taacattaca ccaaaacaat gcagatacca ctggtactca agaaaaaata actaaagaca
      301 aagatattgt gttcacaaat gggggaaatg ttctgtttaa ggataatttg gattttggta
      361 gcggtggtat tatctttgac gaaggccatg aatataacat aaacggtcag ggatttacat
      421 ttaaaggagc aggaattgat atcggaaaag aaagcattgt aaactggaat gcattgtatt
      481 ccagtgatga tgttttacac aaaataggcc ccggtactct gaatgttcaa aaaaaacagg
      541 gggcaaatat aaagataggt gaaggaaatg ttattcttaa tgaagaagga acatttaaca
      601 atatatacct tgcaagcgga aatggtaagg taatactaaa taaagataat tcccttggca
      661 atgatcaata tgcggggata ttttttacta aacgtggtgg cacgctagat ttaaatggac
      721 acaatcagac ttttactaga attgccgcca ctgacgatgg aacaacaata actaactcag
      781 atacaacgaa agaagccgtt ctggcaatca ataacgaaga ctcctacata tatcatggga
      841 acataaatgg caatataaaa ctaacgcaca atattaattc tcaggataag aaaactaatg
      901 caaaattaat tctggatggt agtgtcaaca caaaaaatga tgttgaagtc agtaatgcca
      961 gtcttaccat gcaaggccat gcaacagagc atgcaatatt cagaagcaca gcgagtcatt
     1021 gctccctggt atttctttgt ggaacggact gggtcaccgt tttgaaagaa acagagagtt
     1081 catataataa aaagttcaat tctgattaca aaagtaataa tcagcagacc tcatttgatc
     1141 agcctgactg gaaaaccggg gtgtttaaat ttgatacatt acacctgaac aatgctgact
     1201 tttcaatatc acgcaatgcc aatgttgaag gaaatatatc agcaaataaa tcagctatca
     1261 caatcggcga taaaaatgct tacattgata atcttgcagg gaaaaatatt actaataatg
     1321 gttttgactt caaacaaact atcagtacta atctatccat aggagaaact aaatttacag
     1381 gtggcatcac tgcacataac agccaaatag ccataggtga tcaagctgta gttacactta
     1441 atggtgcaac ctttctgaat aatactccta taagtataga taaaggagca aaagttatag
     1501 cacaaaattc catgttcaca acaaaaggta ttgatatctc cggtgaactg actatgatgg
     1561 gaatccctga acagaatagt aaaactgtaa cgccgggtct ccactacgct gctgatggat
     1621 tcaggctgag tggtggaaat gcaaatttca ttgccagaaa tatggcatct gtcaccggaa
     1681 atatttatgc tgatgatgca gcaaccatta ctctgggaca gcctgaaact gaaacaccga
     1741 ctatatcgtc tgcttatcag gcatgggcag agactctttt gtatggcttt gataccgcct
     1801 atcgaggcgc aataacagcc cccaaag
//
LOCUS       DAJVPP010000231         1816 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVPP010000231 DAJVPP010000000
VERSION     DAJVPP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.238"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(198..599)
                     /locus_tag="OQM34_004102"
                     /pseudo
     CDS             complement(198..599)
                     /locus_tag="OQM34_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1028..1816
                     /gene="virF"
                     /locus_tag="OQM34_004103"
     CDS             1028..1816
                     /gene="virF"
                     /locus_tag="OQM34_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCS1454304.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          565 a          329 c          337 g          585 t
ORIGIN      
        1 tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt tattttttac
       61 attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg taagcgatga
      121 gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac ccacgatatt
      181 cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc ccttggtgag
      241 agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc cactgcaagg
      301 ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt catcgttttc
      361 atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt gcagggctcc
      421 agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga accgttatcg
      481 gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat gacatcctgc
      541 accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc acgatcgcaa
      601 tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt aacgcttacc
      661 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
      721 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      781 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      841 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      901 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      961 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
     1021 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
     1081 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
     1141 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
     1201 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
     1261 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
     1321 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
     1381 caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag cttgcctttt
     1441 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
     1501 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
     1561 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
     1621 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1681 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1741 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1801 tcataaaaaa ttttaa
//
LOCUS       DAJVPP010000232         1795 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVPP010000232 DAJVPP010000000
VERSION     DAJVPP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.239"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(78..1682)
                     /locus_tag="OQM34_004104"
     CDS             complement(78..1682)
                     /locus_tag="OQM34_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1454305.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          445 a          452 c          410 g          488 t
ORIGIN      
        1 acaagtagag aaaactatga gtcaaaaaca tgaccacaca ggtctgtagt aagtgtcatc
       61 ctagactatg gtctcctcta ggtctcctta taaacatcag ccatcacatt gaccagccta
      121 tcaacctcga cctctttagc ataacggtcg tacgtaatag accctgaact atgccccatg
      181 atcgcctgtg catacgccaa ctcgacccca tgttgtttta actgggtcgc aactgtatgt
      241 ctaaacgagt gaaacgctaa gccgctacgg tctacgagga ctttaggcaa caacgtccga
      301 ttaaaccatt tactcacctg ctcaccgtaa ccgttcttca tgagacgcag accatcgaac
      361 accatagcgt catcaccgtc agcgcctcta cgggtctcta caagcgccat aaagtcagcc
      421 aatataaaac cataggcccc atcgaccaga ggcacacagc ggtcactatg agcgttcttg
      481 atgctcttac cgggtagact actatcgtct tcattgatat gaatataaac ggttcccgcc
      541 tcagtggtcc tgacgtcttt gacctgtagt tgggctattt cattgagtct cgccccagtg
      601 atagcagcca aggcggtaac ataataatga tacggcttac cagaggtctt ctgagagtat
      661 gccttagctg cgaccagtag ttgccccacc tgctccgtag agaaggcgtt acgggcccct
      721 gaggctttac gctgagggac tttaagctcc agaccctcag tcatgttctt tttaatcaag
      781 tcattgcgca cagcccacct aaagacagca gccatcttaa taagatactt gttgttgatg
      841 gtaacgacat ccagacaatc tgtcttatct tcacggctca acaggtcagc aagtgggaca
      901 tccttgaagc gctgcttacg gttcctaggt aactgctgga gcacatcacg gaccctaagc
      961 atgtcagccc tgttagcgtc tgcattgaga cccagatggt cgaatatttc gattaacgca
     1021 gcgtgtgacg ccttgttttc ccttaaggtt gcaggcttcc agttctgata gttctccgct
     1081 tcgtattgct caaagaggct cgctatggta atgctaggcg taacttctgg ttttacttct
     1141 ggagcattaa cagataggga cacagagtct gcttggttat cttgcctatc aatatcatta
     1201 aaaagatcga cgtaatctat cagaggctga ctgtctcccg cttcgatacg cctcatacag
     1261 gcttttagca ccttcagggc ctcattgatg taacgatgct ggtcaatgct tagcggctca
     1321 ctagccagag catcggtaag gctctcacca agttctccgt attggtcccg gtagatatcc
     1381 ctcatgtcag gctcaaagag atctgaacgg cccatagaca gttcacactc agcgatgtcc
     1441 ttaaggtgtt ccctcatttc ctcgtaggta gcgttgggtc tgtctgcgtg tattgccttg
     1501 agtgctgcct ttatgtgcct tgagtaagcc atagcggtgc gtctatcctt ggtccgtaat
     1561 gatattgagg tcattctatc attgtttgat tgtctgaccc taagacggaa gtagtagacg
     1621 ccgttacgct ggtagagata gtagactgct ggaagtttgt gactactgga tagctgaatc
     1681 atatgtctgt atgccctgag agggttacac agtgggttac agtaagtaac tctgcaagga
     1741 ggcttaaagc cgtatccctt aggattcagt aggttggagt actttaagaa aggtc
//
LOCUS       DAJVPP010000233         1758 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVPP010000233 DAJVPP010000000
VERSION     DAJVPP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.240"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            96..842
                     /locus_tag="OQM34_004105"
     CDS             96..842
                     /locus_tag="OQM34_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1454306.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            857..1279
                     /locus_tag="OQM34_004106"
     CDS             857..1279
                     /locus_tag="OQM34_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1454307.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1337..1693
                     /locus_tag="OQM34_004107"
     CDS             1337..1693
                     /locus_tag="OQM34_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454308.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          475 a          381 c          508 g          394 t
ORIGIN      
        1 cgaaaggtgt tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca
       61 gtgcgatccc gctaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg
      121 ttctggagcg tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg
      181 cggagtggcg cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc
      241 tgaatcgtca gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc
      301 ggaagtgttc gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc
      361 aggcgaaatc catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta
      421 agcccggcac cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg
      481 ggcgtagcgt gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg
      541 acaacggcaa atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc
      601 tggatgaaat tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg
      661 ttgatcgccg gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag
      721 cgatgaatac acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct
      781 gggttaattt taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt
      841 agtttcagga ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac
      901 gcgaattgtg gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtccgatga
      961 aacaggtatc agtcgtgcgt ctgtcggtcg cttatgcata gaactggtcg caagtggtga
     1021 tgtatataat tctggctacg gcttattccc gtctgaacag gctcgcaagg actggcaaag
     1081 cgcccgcaaa aaactctcga gagtaaaggt gaggaaaccg gttgttgttg atccggacct
     1141 tatctggtca ttacctgacg gagaaatacg ccgctacgac aggcgcctga atataatctg
     1201 tcgcgagtgc cggaagagcg aagctatgca gcgtgtactg gctttctatc agggtaattt
     1261 tcaggaggcg atactgtgaa tgaaattagc tatcaggctt caattaccgc tggcattcgc
     1321 atcaaaggag aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt
     1381 tccgataaaa gaggatgacc ctcgtaatca ggttaatttt ctttataagc catcggaaag
     1441 accatattgc catcacgcct ctgtacgggt tgacgaaaaa gagcgtcagg tccgctgtaa
     1501 aatctgcggt gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac
     1561 cagactggca gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat
     1621 agaaaagctg atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc
     1681 cagaactgaa taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc
     1741 tgcaccctca aatcatca
//
LOCUS       DAJVPP010000234         1734 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVPP010000234 DAJVPP010000000
VERSION     DAJVPP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.241"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(25..234)
                     /locus_tag="OQM34_004108"
                     /pseudo
     CDS             complement(25..234)
                     /locus_tag="OQM34_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(284..463)
                     /locus_tag="OQM34_004109"
                     /pseudo
     CDS             complement(284..463)
                     /locus_tag="OQM34_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            987..1271
                     /locus_tag="OQM34_004110"
     CDS             987..1271
                     /locus_tag="OQM34_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCS1454309.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHPLPVPR"
     gene            1271..1546
                     /locus_tag="OQM34_004111"
     CDS             1271..1546
                     /locus_tag="OQM34_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1454310.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          475 a          438 c          409 g          412 t
ORIGIN      
        1 atcgcagctc tgacgactca ctccttattt acccgggaaa tacttctcca gcaatgcata
       61 caggatggca gcggtttcag catccattat gccgtcataa tttttctgcc ggaaatgaag
      121 ctgaaaagcg cggacaagcg ccttatatcc gtcatccgtc tccacaccag aaatactgta
      181 tccataggtc ttcagccgtg acacgatatc aaccttatcc ggaatgccct gcgattcaaa
      241 aaataaatat ttagtcagta attttatgtt tttcctgaaa aaattactct ttttcgaact
      301 ccagtgagcg ttcacgactc atcagtgtga aaatgatggc aaaaacagca gacagtatca
      361 gtacgataaa tgtggccccc catcccagat aatcgacaac aagtcccatt cccagtgtcg
      421 caacaattga gccgaagata taggaaaatg tgcctgacag ccctaacaga ataagtttac
      481 gcgtaaatcg cccggaatcc agaagagcat taagtatcac ataaacaggt tccataccag
      541 tcctgaccgc atcaggacgc cagtaaataa aatctcgtag tccaccggca tcagagacaa
      601 gagtaatcgt cgttaccggt atttcgactg gtttgtactg gtgtgagcgg acaaaactac
      661 tgtacgtctc tcatacacgc tcgtaagcgg ccctcacggc ccgctaacgc ggagatacgc
      721 cccgactgcg ggtaaaccct tgtcgggacc actccgaccg cgcacagagg cattttcatc
      781 gctgacaggc cgcatggcct cgcagagccg gagggggact ggtgatattc ggacgaccgt
      841 accggcttgc gccgggcttc ggcggtttta cacctgtgct acatgtaacc accgtatgcc
      901 gccttatatg cgctgactcg cattatttgc cttgttccgt ctgaaagtaa tcactatgat
      961 taaatatgat taacaactaa tcgggtatgc aaatgaaaaa caataccgca caagcaacaa
     1021 aagtaattac cgcgcatgtg ccattaccta tggctgataa agtcgaccag atggccgcca
     1081 gactggaacg ctcccggggc tgggttatca aacaggcgct ttctgcatgg cttgcccaag
     1141 aggaggagcg taatcgcctg acgctggaag ccctggacga tgtgacatcc ggacaggtta
     1201 tcgaccatca ggctgtacag tcctgggcgg acagcctcag tactgaccat ccgttaccgg
     1261 tgccacgctg atggaactga agtggaccag taaggcactt tctgatttgg cgcggttata
     1321 tgattttctg gtgctggcca gtaaacctgc tgccgccaga acggtgcagt ccctgacaca
     1381 ggcgccggtc attctgttaa ctcatccacg tatgggagaa cagttgtttc agtttgaacc
     1441 cagggaggtc agacggattt ttactggcga gtacgaaatc cgttacgaac ttactggcca
     1501 gactatttat gtattgcgcc tgtggcacac acgagagaac aggtagattg ggtgtggagg
     1561 caactttaac atagcctgca ggtattaatt ctggagcatt gttatcaggc cagatatttt
     1621 gcattccatt aaataatctt gtaggagata aaataaatta cgatgaaata acgaaaatta
     1681 ccgctcgtga ctggcggcaa tatcgcgctc cgggctggca aataacccat caga
//
LOCUS       DAJVPP010000235         1657 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVPP010000235 DAJVPP010000000
VERSION     DAJVPP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.242"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(158..1393)
                     /locus_tag="OQM34_004112"
     CDS             complement(158..1393)
                     /locus_tag="OQM34_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1454311.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1540..1616)
                     /locus_tag="OQM34_004113"
     tRNA            complement(1540..1616)
                     /locus_tag="OQM34_004113"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1580..1582),aa:Pro,seq:tgg)
BASE COUNT          420 a          451 c          394 g          392 t
ORIGIN      
        1 ctgccaccgc acgtaaaggc tttgccggat acgatcaccc aaaccagtat ccacagaacg
       61 ctgcaattta ttgaatttgc acgatcatgt aggccggata aggcgtttac gccgcatccg
      121 gcaatcaacc gcaggcggct tccaaatggc cgctttttta cttattcacc accatcaaat
      181 gtgcctgcat aatgtcactg gccgggcgac cgtgcgccag caaatcagcc attgctttaa
      241 gatatggcgg tagatggcgg aaataacgct gatacccggc acacaaataa ttcagccccg
      301 gtttgccgct ggcatcgagc atgaagcggt gtttcgggca gcctccccaa catgctttta
      361 acacgttaca actgcgacac tgcgccggta attgcttaaa tttatcttca ccaaacacct
      421 gctgttgcgg ggaatcgatc atttctgcaa ttgtttgctg atgcatattc cccagccgat
      481 attgcggata aacatagtga tcgcaggcgt aaacgtcgcc gttgtgctca acaatcaccg
      541 agcgcccaca ggttggctga tgatggcaaa ccgcacccgg cgcaccgaca aaattggcaa
      601 acgcccattc gatattcatc acgaaaatct tgccgacgtc gcgtttgatc cagtggtcaa
      661 aaatcgccac cagaaattca ccaaattcat cggggcgcac agaccattcc gtcagttccc
      721 cctgaatatc accaggcgca tgtagtttca gtccatcgcg gacagctgtt tcatcagcca
      781 ggcgctcgac caccggaata aactggatga attcgactcc cgcatcgcac aaaaaatcat
      841 aaacctgtaa cggttgctgc gcgctggtgc ggttgacgca gaccagcacg ttatagtcga
      901 catgatgttt ttgcaggagc gtcagggcac gcatcaccag tttatgcgtg ggtctgccgc
      961 ctttggtcac gcgatattga ttgtggatct cagccggacc atccagcgat aacccaacaa
     1021 gaaaattatt ttctgccaga aatgcacacc attcgtcatc gagcagcacg ccgttagtct
     1081 ggaaactgtt acttatcttc ctgccagcac catatttcgc ctgtagcgcc acggcacggc
     1141 ggtaaaactc cagcccgagt agcgttggtt cgccgccctg ccaggtaaaa gccacttcgt
     1201 tttgcagttc gctggcggcg atatagtgac ggatatacgc ttccagcgtg tcatcgtcca
     1261 tatgcgtgac tggcttttcg cggtaaaggg attgtttttc gagataaaaa cagtagtcac
     1321 agttcagatt acaatcggaa ccgctcggtt tcgctatcac atgaaaagca cgcgttggaa
     1381 cctgttgcag catggtcgct ccttgcctga agtcatcctt caatacgtta agggcgaaat
     1441 cataccgtgg ggcaatgagt tattacgggc gtttattgcg aagggagggt gacgaaatgc
     1501 acagaaaaca aaaaaccccg ccgaagcggg gttcaaaatt ggtcggcgag agaggattcg
     1561 aacctccgac ccactggtcc caaaccagtt gcgctaccaa gctgcgctac tcgccgaaat
     1621 actgcttttt gaatttttag ttcaattctt taaagtc
//
LOCUS       DAJVPP010000236         1629 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVPP010000236 DAJVPP010000000
VERSION     DAJVPP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.243"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..278
                     /locus_tag="OQM34_004114"
                     /pseudo
     CDS             1..278
                     /locus_tag="OQM34_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            447..>1629
                     /locus_tag="OQM34_004115"
     CDS             447..>1629
                     /locus_tag="OQM34_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1454312.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGVSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          435 a          309 c          357 g          528 t
ORIGIN      
        1 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
       61 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      121 aggactggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      181 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      241 caagaataaa tagtagtaac tactatagtt gcaaataatg ttaaaaacat ttctatgatc
      301 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      361 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      421 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      481 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      541 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      601 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      661 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
      721 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
      781 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
      841 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
      901 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
      961 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1021 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1081 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1141 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1201 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1261 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1321 aggatatggg aaaactggca tcaatggggg gggtttctgt ttttgaaatc ggtggtgtgg
     1381 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1441 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1501 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1561 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1621 gagccgcat
//
LOCUS       DAJVPP010000237         1612 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVPP010000237 DAJVPP010000000
VERSION     DAJVPP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.244"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(321..686)
                     /locus_tag="OQM34_004116"
     CDS             complement(321..686)
                     /locus_tag="OQM34_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS1454313.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            complement(686..1351)
                     /locus_tag="OQM34_004117"
     CDS             complement(686..1351)
                     /locus_tag="OQM34_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454314.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            complement(1348..>1612)
                     /locus_tag="OQM34_004118"
     CDS             complement(1348..>1612)
                     /locus_tag="OQM34_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1454315.1"
                     /translation="VKPVIRFDGNMLHQERDDISNMVPECHPCNLHKHCSSLEDYRRI
                     ISDGRREFLASGKGKALVRMGLVEMKSDPVVFWFEKYQEGATA"
BASE COUNT          409 a          447 c          337 g          419 t
ORIGIN      
        1 agtggcatgc ccgaggcaga cagcctcaag cacccgcagc aatcttaccg ataccgataa
       61 gaaaacgcta ttttttattg ccagcacctt ctatccaggc taatttatct gcgtcagaac
      121 ggcgcaatgc gctcgcctga gatacgttta cttgccatgg taacaacagg taacggcaat
      181 tgctgtttct gttttgtttc cttcaaaaac cccggaccat caatccgggg tttttgtttg
      241 ttaaccccaa cggcaaatcg aatacaccac cagcgccacc gccatcgcaa ttcctaccgt
      301 ggtgaatgcc tcaggccagg tcattgattc acctcctgcg gtggttccgg tagcggcatc
      361 cagtgagttg agctctctac ttcaacgccg tctttgtcca caaaagccat cttattcccg
      421 ccacgagtgg ggcaaaaaac cttatcccag gaaccgggga atacattccc gagatcatcg
      481 agaagaatca catcgcaata ttcagcagga actgcatcac tacagcttat ccagccagcc
      541 agagttaccg gagagttgcc agccttacgc atggccattt ccacgatttc aaccatatct
      601 cctggtggaa ttttacaaag ttgcccaata catttctgct gcctggcata ttcgagaatg
      661 tgctccagtt tggttcgatt aatcattatt tatctccctt aagcatggca gcgcggcagg
      721 cgttccagcc atcaacataa tcaaacgtat tactatcgtt tggcccgatt tcatctggca
      781 ctatcggctc tggttggata gtgacgttgg caaaggcagc acgcaaaccg gttttaattt
      841 cttcgatttc atcagagcca agcgatgaat ctgacagtgc gtgatggaat gcgtaagcca
      901 tgtcgtcgtt tactgcaacc ggcactgccg gcgctgcgta aagcggtgtt atatctgccc
      961 gaaaatcaca tgctttatgc agtcgtaccc aacgctcaac ttccgctttg taaaaatata
     1021 tagcagtgaa cgtattagat tcctggtcaa cttgagtaaa cgtcaccttc cacgctacag
     1081 ctacggcttt ccatccatca ggttgtcgca ttcgcggggc agcataaagc tcatgcattc
     1141 catcaggcag cgagtgaccc acatactcac cgaacccgtc aatacacatg cccccatctt
     1201 caacaatgca ttccgctaca ggctctgctt ccagtgatgc cagagcgata agcgccagtt
     1261 cacgaatttc accgccgtct atatcgtcaa tatcatcgcg gccagaaatg ttagccagcc
     1321 attgcagtcg ctctttggta atagtagtca tgccgtagcc ccttcttgat atttttcaaa
     1381 ccagaacaca accgggtcag atttcatttc aaccaatccc atacgaacca gcgctttgcc
     1441 tttcccggac gcaaggaatt cacgacgacc atcactgata attcgtcgat aatcttccag
     1501 gctactgcaa tgcttgtgca gattgcatgg gtggcattct ggtaccatgt tggatatatc
     1561 gtcacgttcc tggtgaagca tatttccatc aaaacgaatg accggtttta ca
//
LOCUS       DAJVPP010000238         1591 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVPP010000238 DAJVPP010000000
VERSION     DAJVPP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.245"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            230..499
                     /locus_tag="OQM34_004119"
                     /pseudo
     CDS             230..499
                     /locus_tag="OQM34_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            496..722
                     /locus_tag="OQM34_004120"
                     /pseudo
     CDS             496..722
                     /locus_tag="OQM34_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            751..1341
                     /gene="ospG"
                     /locus_tag="OQM34_004121"
     CDS             751..1341
                     /gene="ospG"
                     /locus_tag="OQM34_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCS1454316.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          469 a          328 c          375 g          419 t
ORIGIN      
        1 ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag tgataggaca
       61 ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta ctcctctgcc
      121 atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct ggtgacgata
      181 tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa tggcacaggt
      241 taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg ctgcaaaaag
      301 catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg ttgaacggca
      361 gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg aatgcggtga
      421 tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg aaatgagcag
      481 gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca ggacaggaag
      541 cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg gacgaacgga
      601 ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac ggtcgtgtgg
      661 aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa aatctcttgt
      721 gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat tattcaaaca
      781 ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc cattctcggt
      841 aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga ttcatctgct
      901 ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga aatggcttgg
      961 caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt tatacagtat
     1021 ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag tgacattgat
     1081 acagctgata tccctgataa tatagagagc ctttatctac agttgatatg taaattgaat
     1141 gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga taaagaaagt
     1201 gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc tgcaaccaaa
     1261 aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt ttattcgtta
     1321 ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg ctcttctatg
     1381 agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta tgcaccgcca
     1441 gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa ccacccgaaa
     1501 aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca gcaaggcgaa
     1561 tcacgcacgg atttccagcc agagacacct g
//
LOCUS       DAJVPP010000239         1549 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVPP010000239 DAJVPP010000000
VERSION     DAJVPP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.247"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            351..476
                     /locus_tag="OQM34_004122"
     CDS             351..476
                     /locus_tag="OQM34_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCS1454317.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="OQM34_004123"
     CDS             complement(921..1397)
                     /locus_tag="OQM34_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1454318.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          357 a          405 c          385 g          402 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggt gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcctc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atcctggatg
     1501 cgacaacgtt gtgtcaccct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAJVPP010000240         1512 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVPP010000240 DAJVPP010000000
VERSION     DAJVPP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.248"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            87..281
                     /locus_tag="OQM34_004124"
     CDS             87..281
                     /locus_tag="OQM34_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454319.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1080..1416)
                     /locus_tag="OQM34_004125"
                     /pseudo
     CDS             complement(1080..1416)
                     /locus_tag="OQM34_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          452 a          334 c          319 g          407 t
ORIGIN      
        1 attccaatgc ggataaaaca cgtccaccac actgattaca tttttcatta gcacagtggt
       61 gtgatgcagc atacgaggaa aaccggatga atgagaacaa aataaaaagg ctggagcaac
      121 tgttacaggc acgccaacgg gagtttgcca ctaaatgggg acgtgccgcg ctgccacatg
      181 aacgcctaac ctattttcca cttgaagacc tcattaaaaa taatggcaaa aaaatcaggt
      241 ctgataagaa acacttctta gaatgcaatg aagacaaata acaacaatct gcatattgcc
      301 accaaccaca gatgtggcat cgttatcagc gcacttgatt ttctttatat tcactgacga
      361 gcttgccggg gcacctttgt ttgccggtca ttctggtatc atgcagatat ttacggcgta
      421 caagcccact agcatgacat tgtaataagg cctgagccta acgctttaat gtgtgtcata
      481 cactcagtgg cgtaatcgtt gacaatatgg tgcatgtaat cgtaagcgtc cagcgaaatc
      541 catctggtca taatctgaag catccgacaa agtggtgtcc accaaataag tgatctgcgc
      601 ccaatatcct gagccggaac tttgacaaag gccaacgaaa aatgggagcg gatgttacag
      661 agttctcggt acaggggaaa agtggtacct gtcgccagtt ctccgatctg tttaaccggg
      721 aaattatctc ctacagcttg tcagaaaggt cggtgatgga gcatcgttaa tacgatgcta
      781 tacgatgcat tcacagtgcc cggccagagg atgccccgtc gctgcatatg gatcagggtt
      841 ggcaatatcg aatcgcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat
      901 gccgcacaaa ggaaacggtc tgaataacgc agtgatgaag aacttcttca gcacactgct
      961 aaaacgcata gtgatcgagc gctgattctg gcgaacaact gaactaatac atcagaatat
     1021 gcattatgtt aaataaatat gaaaagatgg tttaaatacc ccgttacttt tgacttacac
     1081 tatacggtat cgcatcgttt aatattcgca ccggccagat ttttatttct attagttgtc
     1141 acaatactga atgcgtacga ccacagtatt ctggctcctg tgtggttatg ctttaattct
     1201 gcgttccggg cagataagca gttgcttgca ggaatccttc ttgtgttaat gtcagttccc
     1261 ctttttacca gtgctgattt ccacattccg tccaacagag cttatagcct ttccctggat
     1321 tatagcattg tccggctgaa gttctttttg aataataata gaagcactgc tggcagatcc
     1381 agtccgtttt tcataaccca ctgtactgat aaccataatc taatcagtag aaattgagtc
     1441 gaaaataagc actactccat acaggataat tagaggtcag tttgattatt cacaattcat
     1501 catcagcatt tt
//
LOCUS       DAJVPP010000241         1469 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVPP010000241 DAJVPP010000000
VERSION     DAJVPP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.249"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..921)
                     /locus_tag="OQM34_004126"
     CDS             complement(<1..921)
                     /locus_tag="OQM34_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCS1454320.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
     gene            997..1428
                     /locus_tag="OQM34_004127"
     CDS             997..1428
                     /locus_tag="OQM34_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS1454321.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT          326 a          360 c          405 g          378 t
ORIGIN      
        1 tcctccgggg aagcgcatgg cgtccgtcat cgacgtcagc agatggcaga cttttccttt
       61 gcgcgtcacg gtcagcaggc gggctgtcac ttcatttccc agtcccggcc acttttttcg
      121 tgcctgcggg ctggttttca gcttcaccag atgatcgcct ttacccagtt ttctgagctc
      181 ttcatattgc gctccctttc tgagaggtat catccagtgg cggtgttctc ccgccaggct
      241 ccagccattt aacagtccca gtgagtaata acctttatcc attaacgtca gagtgttatc
      301 gccggtttgt tctataagtt gctcagcaag ctcattttcg ctgttcttca tcgtgccgaa
      361 ggctgcagcc gtcagcagat ggctggtcag ttccatctgg cagaccattt tgacctgcgg
      421 gtagagcgcc gggttcccgg catgtgtctg gcgggggaag gctgcatcgt tctctggtgt
      481 atccggtgtg cgccagaaca caccatcgat ggccagcagg gtcaggccgc accagtgcgg
      541 atgcggcgtg gcgttatgcc agagctgcgc tgttttcgtg aacacgcggc ggacagcctc
      601 acttcccagg cgctggcggg cctgaataac ggcactgggg gcaacgaagg ggcgattgcc
      661 cggcagcatg atgtccaggc gattcacaat ctggtgaaga ggttctttac gctcaagcgc
      721 catgccaaca atacaccaga ccatcatttc gaggggaaga cggcgcttgc gtagcgttac
      781 agtacctgat tcggcaaggc aacgagagat gagttcgggg tcgaggtaat cccccagaga
      841 agtcagtggg ttacgcagag aatcgtaacg ggataccaga tcaagagcct gtccaatgtg
      901 cataaaaaaa tccggaaaca agtgagcgtt tccggattct tacacagcca ctggatcggt
      961 caactgatcc ttaactgatc ggcattacag cagaaggtgg cgttaaatct cgcttcaggt
     1021 gaagggaatt tctttatccg tcctggcggc gtgttttatg tcgcgggaga taaagtcggc
     1081 atcgttcgtc tggatgcttt taaagccagt aaagatattc agtttgcggt gcagtcagga
     1141 ccaatgttga tggaaaacgg tgtaattaat ccgcgtattc atcccaacgt cgcctcacgt
     1201 aaaattcgta acggtgttgg gattaataaa catgggaatg ccgtgttttt gttgagccag
     1261 caggcgacaa atttttatga ttttgcctgt tatgccaaag cgaaactgaa cgttgagcag
     1321 ctgctttatc ttggtggcac gatttcacat atgtatatga aaggcggggc aatcccgtgg
     1381 caacgctatc cttttgtcac catgatttcc gtcgagcgga aaggttagaa tgatagcttt
     1441 gtgtcagata ctaaatatgc tgctgatgc
//
LOCUS       DAJVPP010000242         1443 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVPP010000242 DAJVPP010000000
VERSION     DAJVPP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.250"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            110..673
                     /locus_tag="OQM34_004128"
     CDS             110..673
                     /locus_tag="OQM34_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1454322.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            755..>1443
                     /gene="yfcU"
                     /locus_tag="OQM34_004129"
     CDS             755..>1443
                     /gene="yfcU"
                     /locus_tag="OQM34_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCS1454323.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEF"
BASE COUNT          424 a          308 c          344 g          367 t
ORIGIN      
        1 catcatacac taaatcagta agttggcagc attaccaaca ttggctattg actgaatcaa
       61 tgtattgcaa tgtttatatt tttaattatt tatagtttaa ggacaaataa tgagtaagtt
      121 tgttaaaaca gctattgctg cggcaatggt gatgggcgtg ttcacctcta cggcaacaat
      181 cgctgcgggt aacaacggta ctgcacgttt ttacggcacc attgaagact cagtgtgttc
      241 tatcgttcca gacgatcaca aactggaagt tgatatgggt gatatcggtg ccgaaaaact
      301 gaaaaataac ggcaccacca cgccgaaaag tttccagatt cgtctgcaag attgcgtatt
      361 tgatactcag gaaacaatga ccactacctt tactggtacc gtttcttctg caaatagcgg
      421 caattattac accattttca ataccgatac cggtgcggca tttaacaatg ttagcctggc
      481 gatcggtgac tctctgggta cctcttacaa aagcggcatg ggtattgacc agaaaatagt
      541 gaaagacact tctaccaaca aaggcaaagc gaagcagaca ctgaacttta acgcctggct
      601 ggtcggcgca gctgatgcgc cagatctggg taattttgaa gcaaatacca ccttccagat
      661 tacttacctg taaggcatac ccccatagcg acactgctat gggggattta aatgtatttt
      721 gaccgaacaa gaataaaaac ggatagaaac gtgtatgcct gaccattctc tttttcgatt
      781 acggattctt ccgtggtgca ttgcgctggc aatgtcaggg agttatagca gtgtctgggc
      841 tgaagacgac attcagtttg attcccgttt tctggaatta aaaggcgaca caaaaattga
      901 tctgaagcgt ttttccagcc agggatatgt tgagcccgga aaatacaatt tacaggttca
      961 actaaataaa cagccattgg cggaagagta cgatatttac tggtatgctg gtgaagatga
     1021 cgcgagcaaa agctatgctt gtctgacacc ggaactggta gcgcagtttg gtttaaaaga
     1081 agacgtggcg aaaaatctgc aatggagcca cgatgctaaa tgcctgaaat ccggtcaact
     1141 ggaaggcatg gaaattaagg ctgatttaag ccagtccgca ttagtcattt cactgccaca
     1201 ggcttacctc gaatatactt atcccgactg ggatccgcct tcacgttggg atgacggcat
     1261 ctccgggatc gtcgcggact acagcatcaa cgcacaaacc cggcacgaag aaaatggcgg
     1321 tgatgatagt aacgagatca gcggcaacgg gacggttggg gttaacctag ggccgtggcg
     1381 tatgcgtgct gactggcaga ctaactatca acatactcgc agtaatgatg acgatgaatt
     1441 cag
//
LOCUS       DAJVPP010000243         1385 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVPP010000243 DAJVPP010000000
VERSION     DAJVPP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.251"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..514)
                     /locus_tag="OQM34_004130"
     CDS             complement(<1..514)
                     /locus_tag="OQM34_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCS1454324.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVF"
     gene            complement(774..>1385)
                     /gene="sodA"
                     /locus_tag="OQM34_004131"
     CDS             complement(774..>1385)
                     /gene="sodA"
                     /locus_tag="OQM34_004131"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1454325.1"
                     /translation="TLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALESLP
                     EFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAAIER
                     DFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGASGFP
                     ILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
BASE COUNT          335 a          368 c          336 g          346 t
ORIGIN      
        1 cgaaaacatg cggttcaaac gaggcaatcc cggcagtgcc cagaataatc atcgtcatga
       61 gcggcccgga ctccaacgac atcaacacaa atgccccagc ttcttctttc gtaccgtact
      121 gctgcatgat ggaagcgtaa agtccgccgt tggtcatatc catcgccgcc accagcgcca
      181 gcgttgaaag tccggcaaag aatccaactt caacaccatg ttccggaata atgcgcgagg
      241 caattgccgc aaccacccac gcgacggcaa ttttagttac caccagcgta ccggatttac
      301 gcagtaccgt tcccgtcgcg cttaatttta ttgacgcccc catgcaaaaa aaccacaccg
      361 ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa tgatccaaaa tatttccccg
      421 cccccggcga gaaggtgtgg cacagtgcgc caaggaatag cgggacgagc atcatccccc
      481 ccgggatttt ctcaatcgag cgttttatct gcatttccat cacctgtcgg catattttta
      541 agggctgatg cgtaattcct caatggagca tcagtgttga tggaaacgaa gatagacatt
      601 catttaatca ataaaagtga tcaaaatcac atcaattgaa acgctgtttt atttttataa
      661 gaccattgtt tttattttga aattgatcac aaaaaaacca ccgccgttgg cgatggttca
      721 ttttttaagc tgatatgcgg cctatacgcc tcattgcagc aggcggcaaa tgattatttt
      781 ttcgccgcaa aacgtgccgc tgcttcgtcc cagttcacca cgttccagaa ctctttgatg
      841 tagtccggac ggcggttctg gaatttcagg tagtaagcat gttcccacac atccaggccc
      901 agaatcggga agccggaagc gccagaaata gcttcaccca tcagcggaga atcctggtta
      961 gcagtagaaa ccaccgccag tttatcgcct ttcagcacca gccatgccca gccggaacca
     1021 aagcgggaag ctgccgcttt ttcaaattct gctttgaagt tatcaacgga gccgaagtca
     1081 cgttcgatag ccgctttcag gtcaccctgc agggtggtgc cttttttcag acctttccag
     1141 aacaggctgt ggttagcgtg accgccagcg ttgttgcgca gtacggtttt cttgtctgct
     1201 ggcagctggt ccagtttggt gatcagctct tcaaccggca ggttggcaaa ttctggcagg
     1261 ctttccagcg ccgcgttggc gttgtttacg taggtctgat ggtgtttggt gtggtggatt
     1321 tccatggtct gcttatcgaa gtgcggttcc agggcatcgt aagcatacgg cagggatggc
     1381 agggt
//
LOCUS       DAJVPP010000244         1297 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVPP010000244 DAJVPP010000000
VERSION     DAJVPP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.253"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..152
                     /locus_tag="OQM34_004132"
     CDS             <1..152
                     /locus_tag="OQM34_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCS1454326.1"
                     /translation="HLFESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAE
                     TIAHH"
     gene            182..1279
                     /locus_tag="OQM34_004133"
     CDS             182..1279
                     /locus_tag="OQM34_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1454327.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSGWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          404 a          293 c          314 g          286 t
ORIGIN      
        1 agcacctgtt tgaatcgctc tgcatcgacc atttgcaacg ccacggtgcc aataaacagg
       61 caatcagcca cgcatttgat gatgatgttg agtttcagga gcgcatggca gaacacatcc
      121 ggtacatggc tgaaaccatt gcccaccact aggttgatat tgatttggag gactaaaatc
      181 tatggcgctt tttcaacgag caataaacac acaggcctat ctaaaagcag gaattatggg
      241 gtttgctggc gacggcaaaa cctacacagc aagcgaactg gctattggtc tggtaatgct
      301 tatgcggcaa cggggaattg aagctggtaa ccgcccggta atgtttctgg acacagaaac
      361 aggctctgac tgggttaaac cccgctttga tgcggaaaat atcgaattat ttacggcaaa
      421 aacccgctcg tttgttgatc tgcttgaagc cattaatgaa gcggaaagca gcggttccgt
      481 aatgatcatc gactccatca gtcacttctg gaccggatta tgcgacgagt atgccagacg
      541 ccgaaaccgt aaacgaggac tggaattttc aggctgggcg tggctcaaac aggagtggcg
      601 acgctttacc gatcgatttg ttaacagtca ggcgcacatt attatgtgcg ggcgtgcagg
      661 atatgagtat gactttttcg aaggtgatga tggcaaacgc cagttggcaa aaaccggtat
      721 aaaaatgaaa gcagaaacag aaaccggtta tgaaccctcc atcctgatcc agatggagaa
      781 gcaaatgaac atagaaaccg gtcaggtgtg gcgcaccgcc agaatactaa aagatcgctc
      841 cacccgtatt gatggacagg tattttcaaa cccaacattt aaaaactttc ttcctcatat
      901 cgagtttctt aacctgggag gtgaacaccc cggcatcgat acctcacggg ataacagtga
      961 attatttgcc aacgacggta caccgacatg gctaaaagaa aagcgtgcaa aagaaatcgc
     1021 acttgatgaa attatcgagt tgctcaacaa gcaccatggg ggcaccagta acgacgccaa
     1081 acgcgcaaaa gctgatttgc tggaaaaaac attcacctcc cgctcatggg aaagaatcaa
     1141 aggtatggac tggccgacaa tcaaagctgg tcgtaatgca ctatggattg aactggaagg
     1201 cgttgaatat gcattcccgg atccacaaac acaaaacgaa acgcaacaag caggatacga
     1261 tgagaatatt ccggtgtaaa accaagtgca ttttgaa
//
LOCUS       DAJVPP010000245         1289 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVPP010000245 DAJVPP010000000
VERSION     DAJVPP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.254"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..1212)
                     /locus_tag="OQM34_004134"
     CDS             complement(<1..1212)
                     /locus_tag="OQM34_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCS1454328.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAIST"
BASE COUNT          299 a          364 c          328 g          298 t
ORIGIN      
        1 cgtactgatt gcgttggtgg tgtttaacgc caccgttgag cgatcgacat cagacatttg
       61 ccagagcggg ttaaactcaa acgtgaaatc gtccggcagc ggcttgccaa gttccgaacg
      121 atgcatgatg tccagtatcc gccgcaccgg aagacgtaaa cgcctctcct gcaacgaact
      181 gatgcgatcg taatagttgg caaggtctgc atcaccggta gaaaatcctt tcggggactg
      241 tccgaacaac cgcaccagtg ggataccaac agcgccacta atctgttctg caaactgcga
      301 aaggatgtca tccagaccac tgaagctgta ctgatgggtt tcaaacttat cccgcgagtc
      361 catgagcgtc atgccttcat tactctggaa ctgtcgaatc aggtcgatat tcttcagcaa
      421 cgcttcatac gcaggaccac caagtgcgat aagctcgcgt agcttctcca cgctgtaggt
      481 acgcaaatgc gccttgtaga ccagctgcgc cgcgccgaca gtagcgctgt cgaacgcggt
      541 aagacgatcc cagatacgct ctacaaccga cattccccat tcgttctcgg tcatcttctg
      601 ctgaaatggc aacgtgacgc catcaaagcg aatcaggcga ctgtgatgaa tgcgccaggc
      661 aggaattccc gttgctgtgg tcaccacatc gtaaaactca ggtttaccca ggtccggccc
      721 catatcttta atgcggcggg tcagtgccgg gtcgaccatc cagcggtcga gcgggagaat
      781 ccccttaaac ttgcccttac cgatggtttc gggtcgcagc ggggtcattg gtgcctgccc
      841 ctcaatcatg atgaaaccca ccgcgccgcc gtagaggcgc gaccatttca gcacgtcatt
      901 cagcgcatcc cagatttgca actcatccag ttgtgattcg agaatgccac gatcttttgc
      961 atcaatttcc gaagtgatgc gaatgccttt gcgggtcata tcatccggga tagcatcgac
     1021 tgcttcgccg atgatccagg atgaacgata ggaccattcc accagcatgc ggttacgact
     1081 ggtgaaatta gcccggtagg tggatgctga gtgctggtta ggtgtctgca tccctactcg
     1141 ggcaataaaa ttctcataac catcagctgt ggcctgcaca gttcgccgca gggcttgttt
     1201 gtttcgtgcc atcaggcctg tctccccagc tgttcccaga tatccagcga tgtatcgatt
     1261 ggcgcgaagg ccataatgaa tgcatcagc
//
LOCUS       DAJVPP010000246         1241 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVPP010000246 DAJVPP010000000
VERSION     DAJVPP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.255"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..823
                     /gene="ccp"
                     /locus_tag="OQM34_004135"
     CDS             <1..823
                     /gene="ccp"
                     /locus_tag="OQM34_004135"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1454329.1"
                     /translation="LYHDPRLSADSTISCAHCHTLNAGGVDGRKTSIGVGGAVGPINA
                     PTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSWDEIIAKLEKDPQLKAQFL
                     EVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENALTAQQKKGYQLFKDNKCA
                     TCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKEERDKLRQKVPGLRNVALT
                     APYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFLHSLNGVYTPYMQDKQ"
     gene            1034..>1241
                     /locus_tag="OQM34_004136"
     CDS             1034..>1241
                     /locus_tag="OQM34_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1454330.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDD"
BASE COUNT          337 a          242 c          312 g          350 t
ORIGIN      
        1 gctgtatcac gatccccgtt tatcggctga tagcaccatt tcatgcgctc attgccatac
       61 gttgaatgcg gggggcgtcg atggcagaaa aacatcgatt ggtgttggtg gcgcagttgg
      121 gccgattaat gcgccgacgg tatttaactc agtatttaac gttgagcagt tctgggatgg
      181 tcgtgcggca acattgcagg atcaggcagg tggaccgccg ttgaacccga ttgaaatggc
      241 gtcgaaatcc tgggacgaaa ttattgctaa gctggaaaaa gatccgcagc ttaaagcgca
      301 gttcctcgaa gtttatccgc aaggtttcag tggcgaaaat attactgatg ccattgctga
      361 atttgagaaa acattaatta cgccggattc cccatttgat aaatggttgc gtggagatga
      421 aaatgcgctg acggcgcaac agaaaaaagg ctatcaatta tttaaagata ataaatgtgc
      481 aacttgtcat ggtggtatta ttctcggcgg acgttccttt gaaccgttgg ggctgaaaaa
      541 agactttaac tttggggaaa ttacggcggc ggatattggt cgtatgaatg tgactaaaga
      601 agagcgtgat aaattgcgtc agaaagtacc cggtttacgt aacgttgctt taacggcacc
      661 gtacttccat cgcggtgacg tgccgacgct ggacggggcg gtgaaactga tgctgcgcta
      721 tcaggtaggc aaagagctgc cgcaggagga tgtggatgat atcgtagctt tcctgcacag
      781 tctgaacggg gtgtacacgc cgtatatgca ggataaacaa taattaattt gatcgcccga
      841 acaggaatgt ttgggcgatt tttattacga taataaagtc tgtttttaat attatcatgt
      901 taaatgttta tattataaaa agtcgttttt ctgcttagga ttttgttatt taaattaagc
      961 ctgtaatgcc ttgcttccat tgcggataaa tcctactttt ttattgcctt caaataaatt
     1021 taaggagttc gaaatggacc agaagctgtt aacggatttc cgctcagaac tactcgattc
     1081 acgttttggc gcaaaggcca tttctactat cgcggagtca aaacgatttc cgctgcacga
     1141 aatgcgcgat gatgtcgcat tccagattat caatgatgaa ttatatcttg atggcaacgc
     1201 tcgtcagaac ctagccactt tctgccagac ctgggacgac g
//
LOCUS       DAJVPP010000247         1235 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVPP010000247 DAJVPP010000000
VERSION     DAJVPP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.257"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..392
                     /locus_tag="OQM34_004137"
     CDS             <1..392
                     /locus_tag="OQM34_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCS1454331.1"
                     /translation="DQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSE
                     RAPRNVGKTAREKAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARK
                     AKKPSAKTQKIAAATKAKRAAKKKVAE"
     gene            572..>1235
                     /gene="rlmB"
                     /locus_tag="OQM34_004138"
     CDS             572..>1235
                     /gene="rlmB"
                     /locus_tag="OQM34_004138"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1454332.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGS"
BASE COUNT          342 a          305 c          364 g          224 t
ORIGIN      
        1 ttgaccaggt agggcaacgc ctgatggggg aatccagcgg ccagacttat cgcctgggcg
       61 atcgcgtgga agttcgcgtc gaagcggtta atatggacga gcgcaaaatc gactttagtc
      121 tgatctccag tgaacgcgca ccgcgcaacg tcggtaaaac ggcgcgcgag aaagcgaaaa
      181 aaggcgatgc aggcaaaaaa ggcggcaagc gtcgtcaggt cggtaaaaag gtaaactttg
      241 agccagacag cgccttccgc ggtgagaaaa aaacgaagcc gaaagcggcg aagaaagacg
      301 cgagaaaagc gaaaaagcca tcggcgaaaa cgcagaaaat agccgcagcg accaaagcga
      361 agcgtgcggc gaagaaaaaa gtggcagagt gatcaatacc ctctttaaaa gaagagggtt
      421 agattgctga caaaatgcgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc
      481 tacataatca cgcaaattca atatattgca gagatcatgt aggccggata aacgtagtgc
      541 atcaggcaaa acgtaaacaa cgagtacatt aatgagcgaa atgatttacg gcatccacgc
      601 agtgcaggcc ctgctggagc gcgcccctga acgttttcag gaagtcttta ttttaaaagg
      661 ccgtgaagat aaacgtctgt taccgctgat tcacgccctt gagtctcagg gcgtggttat
      721 ccagttggca aaccgccaat atctcgacga gaaaagcgac ggtgccgtgc atcagggcat
      781 tatcgcccgc gtgaagccag gacgtcagta tcaggaaaac gatctgccgg atctgatcgc
      841 ttcgctcgat caaccgttcc tgctgatcct cgacggcgta accgatccgc acaacctcgg
      901 cgcgtgcctg cgtagtgcgg acgccgcagg cgttcatgcg gtgattgtgc cgaaagatcg
      961 ctccgcacag ctcaacgcta cggcgaaaaa agtagcctgc ggcgcggcag aaagcgttcc
     1021 gctgattcgg gtgactaacc ttgcgcgcac catgcgtatg ttgcaggaag agaatatctg
     1081 gatcgtcggt acggcaggcg aggcggatca tacactctat cagagcaaaa tgaccggacg
     1141 cctggcgctg gtgatgggcg cggaaggtga aggtatgcgt cgcctgaccc gtgaacattg
     1201 cgatgagttg atcagcatcc caatggcagg aagtg
//
LOCUS       DAJVPP010000248         1222 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVPP010000248 DAJVPP010000000
VERSION     DAJVPP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.258"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            28..>1222
                     /locus_tag="OQM34_004139"
     CDS             join(28..352,352..>1222)
                     /locus_tag="OQM34_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCS1454333.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRAKKLDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRLMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRAC"
BASE COUNT          300 a          296 c          370 g          256 t
ORIGIN      
        1 gtttttagac tagtcattgg agaacagatg attgatgtct tagggccgga gaaacgcaga
       61 cggcgtacca cacaggaaaa gatcgcaatt gttcagcaga gctttgaacc ggggatgacg
      121 gtctccctcg ttgcccggca acatggtgta gcagccagcc agttatttct ctggcgtaag
      181 caataccagg aaggaagtct tactgctgtg gccgccggag aacaggttgt tcctgcctct
      241 gaacttgctg ccgccatgaa gcagattaaa gaactccagc gcctgctcgg caagaaaacg
      301 atggaaaatg aactcctcaa agaagccgtt gaatatggac gggcaaaaaa gtggatagcg
      361 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
      421 cgcgtgcgca gttgcacgtc attctcagac gaaccgatga ctggatggat ggccgccgca
      481 gtcgtcacac tgatgatacg gatgtgcttc tccgtataca ccatgttatc ggagagctgc
      541 ccacgtatgg ttatcgtcgg gtatgggcgc tgcttcgcag acaggcagaa cttgatggta
      601 tgcctgcgat caatgccaaa cgtgtttacc ggctcatgcg ccagaatgcg ctgttgcttg
      661 agcgaaaacc tgctgtaccg ccatcgaaac gggcacatac aggcagagtg gccgtgaaag
      721 aaagcaatca gcgatggtgc tctgacgggt tcgagttctg ctgtgataac ggagagagac
      781 tgcgtgtcac gttcgcgctg gactgctgtg atcgtgaggc actgcactgg gcggtgacta
      841 ccggcggctt caacagtgaa acagtacagg acgtcatgct gggagcggtg gaacgccgct
      901 tcggcaacga tcttccgtcg tctccagtgg agtggctgac ggataatggt tcatgctacc
      961 gggctaatga aacacgccag ttcgcccgga tgttgggact tgaaccgaag aacacggcgg
     1021 tgcggagtcc ggagagtaac ggaatagcag agagcttcgt gaaaacgata aagcgtgact
     1081 acatcagtat catgcccaaa ccagacgggt taacggcagc aaagaacctt gcagaggcgt
     1141 tcgagcatta taacgaatgg catccgcata gtgcgctggg ttatcgctcg ccacgggaat
     1201 atctgcggca gcgggcttgt aa
//
LOCUS       DAJVPP010000249         1202 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVPP010000249 DAJVPP010000000
VERSION     DAJVPP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.259"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..657)
                     /locus_tag="OQM34_004140"
     CDS             complement(<1..657)
                     /locus_tag="OQM34_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1454334.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     E"
BASE COUNT          315 a          185 c          301 g          401 t
ORIGIN      
        1 ttccggcagg tatggaatat cggttaacat attgtcgatt gcccatatgg agtgaatgtt
       61 gtgaggtaga ggggggagtt cactaagaag attccctatt gcgctaatct cttgtaaaga
      121 aaatggtaaa ggtggtaaaa ctgccagccc attacatgat acatctaatg tttccagtag
      181 ttctggtaaa acaggaagag aacataattg attatttgag acatgaagct ccttcaaaga
      241 tatggggagt gtgggtagtg tgattagttg attgtgggac gcattcaata atttaagtcc
      301 ttgaggcaaa gcaggcagtt caataagtct gttatagctg acattaagtt gtgtaaggga
      361 cgcaggcaat ggggagatta agcttaaatt atttttactt atattaattg atttaattcc
      421 cggggggatt tcaggtaatg ttgtcaggcc taattcagac aagtccaggt tcgtctcttg
      481 gttttgtaga catgatacta gtcgctgaaa agcgatgtct cgttgttctt cttgtatgcg
      541 gttatttttc cattcagtcc aatgggttag ataattttca tatgcgcggc tagtgtcgat
      601 tgaataagtg gagaacgaat ttgaaattaa tctgtgattg ttatttgtcg ggagcataaa
      661 tatcagggcg tattctttat gttgagaggt gttggattct tttttatctg ctttgcaatg
      721 ttattggttc ccttcagcag tagacaggaa cttctggagg aggcggatat atagtttgtg
      781 taagcatatt aactccatat gttatatatt gaagaacttc tgctttacac tatcctaaac
      841 tggatcggtt taatgtaaaa aaacgatact tttttggggg gggcaatttt tccttaaggt
      901 atgaaaaaag gagctgaagc tatataatag ctattaatgc cactggtatc agtgaatcat
      961 gtatataaaa aactgtgtta aggaggctgt ccattagcgg ttttttattc ttctggaacc
     1021 gcggcatctt tgcgtatctt tttgtcttct caccgtctgg tcggtgtttt gctgagactg
     1081 ctaatttcct gtctttgcta gtgctgtact ggtactgccc aatcatgatt ggcgggggcg
     1141 ggcggagttg agaccgcaga cacgtcgtat gcaagaacgt gctgcggctg gctggtgaac
     1201 tt
//
LOCUS       DAJVPP010000250         1182 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVPP010000250 DAJVPP010000000
VERSION     DAJVPP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.260"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..265)
                     /locus_tag="OQM34_004141"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="OQM34_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OQM34_004142"
     CDS             complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OQM34_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCS1454335.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          347 a          292 c          180 g          363 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactttt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tattttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactatc ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gtgtctaaaa
     1141 agtgatccac ttaccaccaa cggcctaata tatacagaac gg
//
LOCUS       DAJVPP010000251         1169 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVPP010000251 DAJVPP010000000
VERSION     DAJVPP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.261"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            281..484
                     /locus_tag="OQM34_004143"
                     /pseudo
     CDS             281..484
                     /locus_tag="OQM34_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            503..>1169
                     /locus_tag="OQM34_004144"
     CDS             503..>1169
                     /locus_tag="OQM34_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat family protein"
                     /protein_id="HCS1454336.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPD"
BASE COUNT          282 a          258 c          360 g          269 t
ORIGIN      
        1 ccatttttat atggaggcgg tagcgtgtct gttactagtg gttttatttg catagtgaat
       61 atattgggat tgttaaatac ataactatgt atccgctctg gtcgttgtaa atattgctaa
      121 ttgcttaata ttattagtga ttatggctgg aaagataaag tgtgctgtaa agcggtgatg
      181 ggtattacat tcatgattaa aaaaacagaa cctgtcaata aataattcct ttattaaaac
      241 ggactattta cattatgacg gaatattatc tcaacgaggc ggtttggaaa tacagagcca
      301 gtttctccgg ggtaacgagc aggtgtacca gtgtcaggtg gcatttgtat tgccgggtga
      361 gcgggtcatg atggccttca cctacgccag aaccacaccg ctgaccccgg cagatatgac
      421 gcgctgggct gaaattaaaa agaatctgcg cttcaggatg cggcaggaag tcaggacgaa
      481 ttaatcatac agggaacggg acatggaaga ggcattctgg gcggcaagac agggtgatgc
      541 cctgctgcat acgtcgttta tggcagatgt gctgggggcg gtgctggagg tggccgcaaa
      601 tgtcgttatt gatgccctca ttgtcggcac aacctcgtta ctggcgacgt cagtggggat
      661 cacgatgggg tgcacggccg tcgtgctgac cggttttgtg gcgggtgtgg cgatggtgta
      721 caccggcgtg acagaaaagg tcagcgaagc ctgttcagcc ctcgcaaata tgcttttccc
      781 gcctcagatt gaaggttaca tcctcaccgg ctctggtgac acatggatca acagcaaacc
      841 tgccgcgcgg gcagcggcga ctgcggcatc gcggcaggac atagaggcgc aggaagcgca
      901 ggcgaaggca gaacaggaag aagcgcagcg tcaggaggat gccagaacgt tccgggatgt
      961 ggccggggaa tatctggaaa tggcgggggc catagccctg gcggttaacc cggtgacagg
     1021 gcctgtgctg atgtcccggg ctctgcacga ccagctcagt acggaagaag ggcgttctga
     1081 aatggtggat ggcgtgacac attttgtgtc ggaactatgg cagcccactg tggccagtgc
     1141 ggccccggga tccaccccga cccctgacg
//
LOCUS       DAJVPP010000252         1165 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVPP010000252 DAJVPP010000000
VERSION     DAJVPP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.262"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(123..890)
                     /gene="ssuB"
                     /locus_tag="OQM34_004145"
     CDS             complement(123..890)
                     /gene="ssuB"
                     /locus_tag="OQM34_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1454337.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(887..>1165)
                     /locus_tag="OQM34_004146"
     CDS             complement(887..>1165)
                     /locus_tag="OQM34_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1454338.1"
                     /translation="VILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNA
                     REFLQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT          281 a          367 c          273 g          244 t
ORIGIN      
        1 tggataaggc gttcacgccg catccggcag ccgtgcccaa ctgcctgatg cgatgcttga
       61 cgtgtcttat caggcctaca agtccgttga aaatgctggt tttgtgcgcc acatccggca
      121 tattaaccat gacgccgaat cagttgttca gaatgcccac gccgcattac ccgttgcaat
      181 acttccgctt ccagctccgc taatcgcacc gaccctaatc gacgcggacg ggggatatcc
      241 actgtcaaat ccagactaat ttttccctct tcaattaaca acacccggtc agccattgct
      301 accgcttcgc tcacatcatg cgtcaccaac agtacggtaa agccgtgctg ctgccaaagt
      361 gacacaatca aatcctgcat ctcgagtcgc gttaaggcgt ccagtgcccc gagcggttca
      421 tcaagcaaca ataaacccgg tcgatgaatc aacgctcttg ccagcgccac acgctgtttc
      481 tgcccgccag aaagtgcggc aggccattcc ccggcgcgat tctccagtcc tgccgcagcc
      541 aacgcccgac gcgcggcatc gcgccactga cctttaaggc ctaacccaac gttatcaatc
      601 accgatttcc acggcagcag acgcgcatcc tgaaacatca ttcgcgtatc atcctgaatt
      661 tcagccagcg gtgtggtgcc cgctaacaca tcgcctgcgg ttggcgtttc cagaccggcc
      721 agcaggtgca gcagggtact tttaccacca ccgctgcggc ccaccaccgc tacaaactga
      781 cctgccggaa tatgtaaatc cagttggttc aggacgatat tttccgcgta atgtttgctt
      841 actgcattga gcaacaatgg tgtgccctgg ttcagacgag cagtattcat atcgtggcct
      901 ccttcaaatg ataagccggg ttccagcgca accagaggcg ctctaacagc tgcgcgctga
      961 cgtcagcaag tttgccgagc agggcgtaaa gaataatagc gactaccacc acgtctgttt
     1021 gcaaaaactc tcgcgcattc atcgccagat aaccaatgcc ggaattggca gaaatggttt
     1081 cggcaacaat cagcgtcagc cacatcaggc ccaacgcaaa acgcacgccg accataattg
     1141 agggcagggc atcgggcagg atcac
//
LOCUS       DAJVPP010000253         1112 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVPP010000253 DAJVPP010000000
VERSION     DAJVPP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.263"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..593)
                     /locus_tag="OQM34_004147"
     CDS             complement(<1..593)
                     /locus_tag="OQM34_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1454339.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRS"
     gene            complement(595..873)
                     /locus_tag="OQM34_004148"
     CDS             complement(595..873)
                     /locus_tag="OQM34_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454340.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            814..999
                     /locus_tag="OQM34_004149"
     CDS             814..999
                     /locus_tag="OQM34_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454341.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(1041..>1112)
                     /gene="hokD"
                     /locus_tag="OQM34_004150"
     CDS             complement(1041..>1112)
                     /gene="hokD"
                     /locus_tag="OQM34_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1454342.1"
                     /translation="LCEVRIRTGQTEVAVFTAYEPEE"
BASE COUNT          250 a          301 c          315 g          246 t
ORIGIN      
        1 attgagcgga ttttttccgc acgcaacccc agcgaacgac gtaataccgc ttccggtagc
       61 gcgtccgcca cctgattgcg gaccgcccac caggataatt cagctagaga taattcacgc
      121 tcctgcgtac cgcttattgc gtgaccgatg acgtcaatca tccatgctga caggttttga
      181 tgagcaagtt gctcgagtga ttcggatgtc tggtcacgca gctggttgtc gcagtgccag
      241 cacaatacca ttgcgccggt accataacgg tgaatgacgg tttcgctgtg atgataatcg
      301 ccgtgtggcc actggcagga tttaatatgg cgcaacagcc agtcagacaa tgcgccagca
      361 ccaccagcag cacgaatcac ccgtgcgtta ctgaaaaacg gcagcaatgt tttgtcttcc
      421 actagcggct ggcgaacggc aggaacgacc ccggacggca gattacgcat gcttttcggt
      481 tccggctcca ccagtacccg ggtattgtgg aataccggca tggattcacg accgggctta
      541 aggaccacca gcccgagttc cggaaccaga acaggtcgaa gtaatacccg cacattacct
      601 ccagatccgt tgctggaatg tgcaggacgg acgcggtggc cgttcggaat aagggagcct
      661 gacgaagatt atccagtgac gataatcgag actgagggct ttcttaatct cgtatccgtg
      721 tctgcggtag cgctgaatca gccattcagc ctgttcttcg gtgcaggggt cgtgctgata
      781 cccgtcagat ttgaatgcat gagaacgccg cccgtgcctg ctggcagggg cggcagagtt
      841 atccgaattg taaaatttgg tatcgtgcgc catctgtttt ctctgctggc gcagcaggtg
      901 ccagttgttc aggctgacgg atggattgta aaccagaacg accagaaaaa acaaaacccg
      961 ccgaagcggg ttaagtgcgg gtgcgttgag gatgcctgac acatcagcgg tggcgaggga
     1021 tttctccccc gccgggtctc ttactcctca ggttcgtaag ctgtgaagac agcgacctcc
     1081 gtctggccgg ttcggattcg tacctcgcag ag
//
LOCUS       DAJVPP010000254         1098 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVPP010000254 DAJVPP010000000
VERSION     DAJVPP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.264"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>1098
                     /locus_tag="OQM34_004151"
     CDS             <1..>1098
                     /locus_tag="OQM34_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCS1454343.1"
                     /translation="IKDDDHHFDARREKKFSRPDRKKIARYVTRTESHLEYLQSRGIS
                     PEVVKRYEVVSGKVWNGERELDALVLPYKRDGELLQVKRISTERPDGKKVIMAEGDCE
                     PCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPFGGGKGAKQQWIEFEYHNL
                     DRFEEIFISMDVDDVGREAAREIVSRLGEHRCRLVTLPYKDINECLMNGVTEDEIWQY
                     IGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWESLADKFQFREAELTLVNGV
                     HGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVTGIFPQGVRDVYRVTF
                     EDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDT"
BASE COUNT          282 a          239 c          319 g          258 t
ORIGIN      
        1 tatcaaggat gacgatcacc atttcgatgc cagacgtgag aagaaattct ccagacctga
       61 tcgtaagaaa atcgcccgct acgttaccag aacagaatcc catcttgagt acctgcaatc
      121 gcgtggcata tcgccagaag tcgtaaagcg ctacgaggtt gtcagcggca aggtgtggaa
      181 tggagaacga gaactggatg ctctggtgct tccgtacaaa cgcgatggtg agttgttgca
      241 ggtcaagcga atcagcactg agcgcccgga cgggaagaaa gtcattatgg cagaaggtga
      301 ttgcgaacct tgcctgttcg gatggcaggc tctggacgct ggcgtgaggg cggttgtgct
      361 ttgcgaaggc gaaattgatt gtatgagcta tgcgcaatac ggcatctcgg cgttatccgt
      421 gccgtttggt ggcgggaaag gcgctaagca acagtggatt gagtttgagt atcacaacct
      481 cgacaggttt gaggaaatat tcatctcgat ggacgttgat gatgttggtc gtgaagccgc
      541 aagggaaatc gtaagccgac tcggtgaaca tcgttgccgt cttgttactc tgccgtacaa
      601 agacatcaac gaatgcctga tgaacggtgt taccgaggat gaaatctggc agtacatcgg
      661 cacggcatcc tacttcgatc ctgaagaact ctacagtgcg cgagagtttt accaggacac
      721 tatcaacgct ttctacggca agcagcagta tctgtttaat ccaccgtggg aatctctggc
      781 agataaattc cagttccgtg aggcagagtt gacgctggtc aatggtgtgc acggtcacgg
      841 aaaggcatgc ccactgaatg agcctattct tttagctgat gggacatgga ctactcacgg
      901 gaatgtaaaa attggcgatc aggtggcgtc agtagacggc aatccgtcaa ctgtcactgg
      961 gatattcccg cagggtgtta gagatgttta ccgagtcaca tttgaagatg gtcgttatgt
     1021 tgattgcgca ggcgatcacc tatgggaggt cactagtcgt ggattcacga aaggcgagaa
     1081 acgccgcgtg atagatac
//
LOCUS       DAJVPP010000255         1071 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVPP010000255 DAJVPP010000000
VERSION     DAJVPP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.266"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            179..>1071
                     /locus_tag="OQM34_004152"
     CDS             179..>1071
                     /locus_tag="OQM34_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1454344.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYN"
BASE COUNT          276 a          245 c          279 g          271 t
ORIGIN      
        1 ttttcgcacc attgcggggc gctgttttta ttatttcctg tctacactca gcttattgcg
       61 acgcgaaatc gtgcgcaaaa gctggcacta cttttgctta taagaggatg gccacagaca
      121 ggtaaaagac gtttcccaaa cgtcctataa cgataatttt cgcctcacag gaagcattat
      181 gaaaaagatg atgatagcca cactggctgc cgccagcgtg ctgcttgccg ttgcaaatca
      241 ggcgcatgct ggcgcgacgc ttgatgccgt tcagaaaaaa ggttttgtgc aatgcgggat
      301 cagtgatgga ttacctaggt tctcttatgc cgatgcagac ggtaagtttt caggtattga
      361 tgttgatgtt tgtcgtggtg ttgccgctgc tgtatttggt gacgacacga aagtgaaata
      421 taccccactc acagcaaaag aacgcttcac cgctttacag tcaggggaag tggatttgct
      481 ctcccgtaat acgacctgga cttcatctcg cgatgccggg atgggaatgg catttaccgg
      541 cgtcacttat tacgacggca ttggcttcct gacgcacgat aaagcggggc taaaaagcgc
      601 gaaagaactg gatggcgcta ccgtctgtat tcaggcgggt actgataccg aactcaacgt
      661 cgccgactat ttcaaggcaa acaatatgaa gtacacacca gtgactttcg atcgctctga
      721 cgaatcagcg aaggcactgg aatctggtcg ctgcgatacg ctagcttcgg atcagtcgca
      781 actgtatgcc ttgcgcatca aattaagcaa cccggctgaa tggattgtct taccggaggt
      841 tatctctaaa gaaccgcttg ggccggtagt tcgtcgtggc gatgatgaat ggttctcgat
      901 tgtacgctgg acgcttttcg ccatgctaaa tgctgaagag atgggcatca attcccagaa
      961 cgtcgatgaa aaagcggcta atccagcaac gcctgatatg gcacatctgt tgggtaaaga
     1021 aggcgattac ggcaaggatc tgaagttgga taataaatgg gcctataaca t
//
LOCUS       DAJVPP010000256         1064 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVPP010000256 DAJVPP010000000
VERSION     DAJVPP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.267"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            24..>1064
                     /locus_tag="OQM34_004153"
     CDS             join(24..273,273..>1064)
                     /locus_tag="OQM34_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HCS1454345.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAH"
BASE COUNT          291 a          262 c          301 g          210 t
ORIGIN      
        1 ctcagaacaa cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg
       61 aatccgctca actggttgtt gaccagaaat acacggtggc agatgccgcc aaagctatgg
      121 atgttggcct ttccacaatg acaagatggg tcaaacaact gcgtgatgag cgtcagggca
      181 aaacaccaaa agcctccccc attaccccgg aacaaattga aatccgtgag ctcaggaaaa
      241 agctacaacg cattgaaatg gagaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt
      301 cagactccct gaacagttct cgataatcgg gaaactcaga gcgcattatc ctgtggtcac
      361 actctgccat gtgttcgggg ttcatcgcag cagctacaga tactggaaaa accgtcctga
      421 aaaaccagac ggcagacggg ctgtattacg cagtcaggta cttgagctac atggcatcag
      481 ccacggttcg gccggagcaa gaagcatcgc cacaatggca acccggagag gctaccagat
      541 gggacgctgg cttgctggca ggctcatgaa agagctgggg ctggtcagct gtcagcagcc
      601 gactcaccgg tataaacgtg gtggtcatga acatgttgct atccctaact accttgaaag
      661 gcagttcgcc gtgaccgagc caaatcaggt gtggtgcggt gatgtgacct atatctggac
      721 gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg ttcgcaagaa aaccagtggg
      781 ctgggccatg tcgttctcgc cggacagcag gctcaccatg aaagcgctgg aaatggcatg
      841 ggaaacccgt ggtaagcccg gcggggtgat gttccacagc gatcagggca gtcattatac
      901 gagcaggcag ttccggcagt tattgtggcg ataccagatc agacagagta tgagccggcg
      961 cggaaactgc tgggataaca gcccaatgga acgcttcttc aggagtctga agaacgaatg
     1021 gatgccggtg gtgggttacg taagcttcag cgaggcagct cacg
//
LOCUS       DAJVPP010000257         1057 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVPP010000257 DAJVPP010000000
VERSION     DAJVPP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.268"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..444
                     /locus_tag="OQM34_004154"
     CDS             <1..444
                     /locus_tag="OQM34_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1454346.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            445..810
                     /locus_tag="OQM34_004155"
     CDS             445..810
                     /locus_tag="OQM34_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1454347.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            807..>1057
                     /locus_tag="OQM34_004156"
     CDS             807..>1057
                     /locus_tag="OQM34_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1454348.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPV"
BASE COUNT          293 a          246 c          293 g          225 t
ORIGIN      
        1 ccgggagagc agaccgccac cagcatactg aagcagcgca caaaaaatct tgcgccgctg
       61 cctcacgccc accagcaaca gaatccacca caggaagaga cggtggtcag cattgccgtt
      121 gatccggaat ctccggctca gtatctccag cgccagaaac cacaacggga agagatgcct
      181 gtatacacgc gctgggtaaa aacgcagaaa tgcatgacgt gtggcaatca ggcagatgat
      241 ccgcaccaca tcattggtca tggactggga gggatgggaa caaaggctga cgatttgttt
      301 gttattccgc tgtgtcgtaa atgccataac gaactacacg ccggggtaaa agattttgaa
      361 gaaaaacacg gtagtcagtt gttgttgctg attcgttttt taatgcacgc gagaaattcg
      421 ggtgttctga agtggaaagc ataaatgacc gaacgcatag aatttgtttt gccttacccg
      481 ccaacggtga acacctactg gcgacgtcgt ggcagcacat attttgtatc aaaagccggt
      541 gagcgttatc gccgtgatgt ggcgctaatt gttcgccagc agcgactgaa attaaacctg
      601 tccggaaggc tggcgataaa gattattgca gagccaccgg ataagcgccg tcgtgacctg
      661 gacaatatcc tgaaggcacc gctggatgcg ctgacgcatg ctggactact tatagacgac
      721 gagcagtttg atgaaatcaa tattgtgcgc ggtcagctcg ttcctggtgg gcggctgggg
      781 ataaaaatca cagaactggg gtgcgcatga ataaccagta tttacagttt gtgcgtgagc
      841 agctcattat cgccaccgct gatttgagtg gggcaacaaa aggtcagctt gaagcctggc
      901 aagagaatgc catgttcgat acagggcgtt acaggcgtaa aaaaatccgg taccgcgatg
      961 aagtgactgg aaaaatgata acgcgggata atccaccaat cccgggaaag caatcgctgg
     1021 cgaaggggac gtcaattcct ctggtcagtc cggttga
//
LOCUS       DAJVPP010000258         1041 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVPP010000258 DAJVPP010000000
VERSION     DAJVPP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.269"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            112..459
                     /locus_tag="OQM34_004157"
                     /pseudo
     CDS             112..459
                     /locus_tag="OQM34_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            814..>1041
                     /locus_tag="OQM34_004158"
     CDS             814..>1041
                     /locus_tag="OQM34_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1454349.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESA"
BASE COUNT          296 a          248 c          240 g          257 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca ctcccccgct
      121 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
      181 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
      241 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
      301 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
      361 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
      421 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
      481 ggtcacatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
      541 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
      601 ctttagaata atcaaagcgt tggattgcca tgtcatatga aaatcggcaa agttcatatg
      661 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
      721 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
      781 tactgtataa aaacacagtg tagagggatt tttatgcgtt ttgaaatctg catagccaaa
      841 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
      901 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
      961 ctttctgtta cacgcaccgc agataaggat tctgcaaaaa catttgttca ggagactctg
     1021 aaagatacct gggagtctgc t
//
LOCUS       DAJVPP010000259         1017 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVPP010000259 DAJVPP010000000
VERSION     DAJVPP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.270"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..638)
                     /locus_tag="OQM34_004159"
     CDS             complement(<1..638)
                     /locus_tag="OQM34_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCS1454350.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHD"
BASE COUNT          336 a          225 c          187 g          269 t
ORIGIN      
        1 atgtcatgca acagatgttc gttaaaaccg aacttattac gcaccgcttc gaataacttc
       61 ggcgtaaaat cgagatactt ttctgaagac cacaactgct cttctggcca caggcctttg
      121 gttgccggtt catacgccag tcctttgccc ttcgccaggc cataagtggt ttgcataccc
      181 ggtacaccgc actgcacacg aatcgcctta aagcccatct gctggtgctt tgcatagtct
      241 tcaaggactt catcaatcgt acgcccggtg gtgtggcaat aaaccatcac gccttcacga
      301 gaagcaccac caagcagttg atacaatggc atatttgccg ctttagcttt gatatcccac
      361 aacgccatat caatggcaga aatggctgac atcgtgacgg ggccacgtcg ccagtaagca
      421 cctttataaa agaattgcca gatatcttcg atacggctgg catcgcggcc aattagttgt
      481 ggacaaagat gatctttcag ataagaagcg accgacaatt cgcgaccatt taacgtggca
      541 tctcctaatc cacaaagtcc gctttcagtg gttattttaa gcgtaacgaa attacgccct
      601 gggcaagtaa caaaaacatc tgcagcaata attttcatta ttgcaatctc ctgtgttgta
      661 aatagacgtt tcaaacctaa ttttacgcct actaccatac aagagagaaa aagcgattat
      721 tcaaccgtga tcacattaaa aacaaaaatt tccaccgagg agaaaaaata ccctcaaaaa
      781 ccacctgaat aattaaccat tattgttaaa acaacacgtt aaataatcac ataacaaact
      841 ccattaatca ttgaaaatga gcacagaaat agaaattaat tatattcaat taattaaaat
      901 gtgtattgct tgatgtattt aaaaatatta ttttcatggt gagagtggag gtaaagaaga
      961 cacaataata aaaaggaatg tgacaaataa tggttctggt tgggcgttaa atttaag
//
LOCUS       DAJVPP010000260         1008 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVPP010000260 DAJVPP010000000
VERSION     DAJVPP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.271"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..1008
                     /locus_tag="OQM34_004160"
                     /pseudo
     CDS             1..1008
                     /locus_tag="OQM34_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          236 a          250 c          327 g          195 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatgcgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt
      961 ggcgaaagtg tgctatgcac aaatggtgaa acagttcctg agtcgtga
//
LOCUS       DAJVPP010000261         1006 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVPP010000261 DAJVPP010000000
VERSION     DAJVPP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.272"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(260..925)
                     /locus_tag="OQM34_004161"
     CDS             complement(260..925)
                     /locus_tag="OQM34_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1454351.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          253 a          251 c          227 g          275 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcatg
       61 taagaggcta acctacatgt gcaaagcatg aaattggcct cagattaatg ttaagcgtct
      121 tgccggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 aagcagatag cctcaagcac ccgcagtcat tctacttacc tcgccaatat gaaatcaatc
      241 agaaaggtgc cccataaaat cactccttct cttctttacc gtagtggagt tgaccaattt
      301 tgataagagg gcgtccctga gatttgcggt gtagattggt atcgcgcaga gaatacacac
      361 agccacaata ttcctgctga tagaattttt cgcgcttgct gatttcaatc atacgggacg
      421 agccgccctg cttgcgccag ttataatccc agtacaccat gcccggataa tgcgcgacgg
      481 ctcgccgccc acagtcgtta acctgctgca tatttttcca gcgtgaaatg cccagtgaac
      541 tgctgatcac actgaaacca ttttcagcgg cgtacagcgc tgtccgctca aaacgcatgt
      601 caaaacacat ggtgcaacgg atccctcgtt cgggctccca ttccattcct ttggctcgtt
      661 caaaccagtt gtctgtgtcg taatcagcat caataaacgg cacgccgtgt tgttcagcaa
      721 agcgaatatt ctcatcctta cgaattaaat actctttctg aggatgaatg ttcgggttgt
      781 agaaaaagat ggtgtagtcg attcccgagg cctgaagcgc ctccatcacc tcaccggaac
      841 atggagcaca gcaagagtgc agtagtagtt tgtttgcccc gtttgggagc tccaatttgg
      901 gccgtttgaa atcagcaact gtcataaata tgtttattga ggtcataaaa atatcaaaga
      961 gtgtagcatt agaacggggc tatcggaaac agatgtgtga ctgctc
//
LOCUS       DAJVPP010000262         1005 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJVPP010000262 DAJVPP010000000
VERSION     DAJVPP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.273"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..546)
                     /locus_tag="OQM34_004162"
     CDS             complement(<1..546)
                     /locus_tag="OQM34_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCS1454352.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINY"
     gene            complement(497..985)
                     /locus_tag="OQM34_004163"
     CDS             complement(497..985)
                     /locus_tag="OQM34_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454353.1"
                     /translation="MRTDPPTNPFQPGNQQALKHGGYARRLLLKDEVIEDAKALTLED
                     ELFRLRANNLVAAENIGRWLVSLEDANGDQERKMLIENISAAEKAMMRNTVRIESIVG
                     TLATVGKIFADTAYRKAATDKVSLEADRLRRDAGIDDGNGERDLNDFYSDIQTDAESG
                     FT"
BASE COUNT          253 a          259 c          230 g          263 t
ORIGIN      
        1 atagttaatt ttgcggatga gagtgccttc gggcggatca acaacgaagt tgcgccagac
       61 gaaatcagta acaagtccgg ggttgaatat gaaccagcat tccgaaccct ctttacggat
      121 cgttggctcc agaattttcc actggtattc cgtcagtgcg tgggcttctt ccagccacaa
      181 catatcgatc ccctccagtg acttaatttc ttcgatgttg cgccataatc cataaaaaac
      241 aaattccgag ccagtaaccc ggttaatgat tttgttgttc agaatacgga aacggtgccg
      301 cagaccaaac ctgtctatct gaattttgag cagggtatac accgactctt cgattttatt
      361 ctggatctga cgggcacaac aaaaacgcag ggtgtattta ttcgacagaa atatggcaat
      421 gccagcggca tcccatgatt ttgacgatga ccgaccacca taaagcactt tgttacgtgc
      481 ccgcatagtc cagaaactac gtaaagccgg attcagcgtc ggtttggatg tcagagtaga
      541 agtcattgag gtcacgctct ccattgccat catcaatacc tgcatcacgg cgaagacgat
      601 cagcctccag agacacctta tcagtggcgg ccttgcggta ggctgtatca gcaaatattt
      661 ttcctaccgt cgcaagcgtg ccaacgatgg actcaatacg aactgtattg cgcatcattg
      721 ctttctcggc ggcgctgata ttttcaatca gcatcttcct ttcctggtcc ccattagcat
      781 cttccagcga caccaaccac cgaccaatat tctctgcagc gacaaggttg ttagcacgaa
      841 ggcgaaataa ttcgtcctcg agcgtcaacg ctttagcgtc ctctatcacc tcatctttga
      901 gcagaaggcg acgggcataa ccaccgtgtt ttaatgcctg ctggttaccg ggttggaatg
      961 ggttagtcgg gggatcggta cgcattccgc gtatcggttt cgtat
//
LOCUS       DAJVPP010000263          994 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVPP010000263 DAJVPP010000000
VERSION     DAJVPP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.274"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(92..604)
                     /locus_tag="OQM34_004164"
     CDS             complement(92..604)
                     /locus_tag="OQM34_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCS1454354.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            799..>994
                     /locus_tag="OQM34_004165"
     CDS             799..>994
                     /locus_tag="OQM34_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454355.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSR"
BASE COUNT          312 a          202 c          241 g          239 t
ORIGIN      
        1 tagcggtaat tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa
       61 gctggattcg caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa
      121 aactaccaac ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt
      181 tcactggatg aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga
      241 taaagcgcga agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt
      301 ctattgtatt ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta
      361 taccaagatg gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag
      421 cttcccatgg tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg
      481 ctggtggcaa tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa
      541 gcccccatag gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca
      601 tcaatgtctt tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta
      661 ggcttaaaca ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc
      721 aggagtgagt gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa
      781 atcacaggaa gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg
      841 gccggagtcc ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc
      901 aggaagaggg ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat
      961 ggtacgcaaa aaaggaaagt gcgtaccagc agaa
//
LOCUS       DAJVPP010000264          991 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVPP010000264 DAJVPP010000000
VERSION     DAJVPP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.275"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..269
                     /locus_tag="OQM34_004166"
     CDS             <1..269
                     /locus_tag="OQM34_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1454356.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..616
                     /gene="tnpB"
                     /locus_tag="OQM34_004167"
     CDS             266..616
                     /gene="tnpB"
                     /locus_tag="OQM34_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1454357.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            613..>991
                     /locus_tag="OQM34_004168"
     CDS             613..>991
                     /locus_tag="OQM34_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1454358.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPA"
BASE COUNT          270 a          258 c          273 g          190 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcacca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gcaacatccc aaacggacag aacggcctgg catccggata taacccgtga
      601 taaaacaagg gaatgaacaa tgaactcccc gatgatattg agctgcttaa agccatgttg
      661 cgtaagcaac agagtcggct tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa
      721 attgaacggc tgaaagcgca actcgacagg ttgcgtcgta tgttgttcgg ccagagttca
      781 gagaaaaagc gtcataagct tgaaaatcag atccgacagg cagaaaaacg actgtcggaa
      841 ctggaaaacc ggctgaacac agccagaaat cttctggaag atgcatcgtc agtcacagat
      901 tcacctgaca ccagtccccc gtcagaaaac ccgatcgcca gtaagcctga atccccggga
      961 cgaaaatctt cacgaaaacc gctgccggca g
//
LOCUS       DAJVPP010000265          987 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVPP010000265 DAJVPP010000000
VERSION     DAJVPP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.276"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(24..827)
                     /gene="dkgB"
                     /locus_tag="OQM34_004169"
     CDS             complement(24..827)
                     /gene="dkgB"
                     /locus_tag="OQM34_004169"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCS1454359.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          223 c          223 g          282 t
ORIGIN      
        1 cccggaggag ctgtcagaga ggcttaatcc cattcaggag ccagaccttc cgggctaacc
       61 aggcggtcgt tgcaatccag tgcggcgatc gcttttttat cttcggcatc aagctgtaaa
      121 ttttgtgcct taagattact ttccaggttt ttacgtttag tagaagaagg aattactgag
      181 taaccttccc ccatagccca cgccagaatc acttgtgccg gaatcgcatt gtgtttagct
      241 gcaatacgag caataacctc atctttcagt gccttaccat aagccagcgt catataggaa
      301 gtgatatgaa taccgtgctg tttagcccag gcaaccactt tacggttttg cagataagga
      361 gagagttcaa tctggttggt agcgatgttt tcagcgccaa cagcagcaat cgctttttcc
      421 atcaatggga tcgtgaagtt ggaaataccg atctcacgcg tcagcccttg ttttttggct
      481 tccagcagcg cctgcataaa ctcttcaaca gagacttcat cgtttggtga cggccagtgg
      541 attagcgtca gatcaacata atcggtacgc aatttttgca ggctctcttt cagactcggg
      601 atcaatttgt ctttgctgag attttcaatc cagattttag tggtgatgta gagttcatga
      661 cgtggcacgc cactttctgc aatcgcctga cctactgcgg cttcgttatc atagatttgt
      721 gcggtatcaa ttgcgcgata accaagttca agcgccgttt tcacagatga aataacaacg
      781 tcgtctttca gacggaaagt acctaaacca aatgcaggga tagccataat atgcctcttt
      841 taatgctatt ttcgttaact gaggtgatta tgggcttcag actgttgcgg aaaaagagcg
      901 taaaacgcag aggatttttg cgattctagc aataatagat atccagacgc aaaaaaacat
      961 cgagcgttaa ctcgaggctt cttaata
//
LOCUS       DAJVPP010000266          987 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVPP010000266 DAJVPP010000000
VERSION     DAJVPP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.277"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..172
                     /locus_tag="OQM34_004170"
     CDS             <1..172
                     /locus_tag="OQM34_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454360.1"
                     /translation="AGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVL
                     DVSDISVKLQEA"
     gene            174..779
                     /locus_tag="OQM34_004171"
     CDS             174..779
                     /locus_tag="OQM34_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCS1454361.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            766..>987
                     /locus_tag="OQM34_004172"
     CDS             766..>987
                     /locus_tag="OQM34_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1454362.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAI"
BASE COUNT          231 a          233 c          269 g          254 t
ORIGIN      
        1 tgccgggcgc tactatgccg gcatttacaa aaccgatccg gaacatattg atattctggg
       61 ccttactgtg agccgtgatg gttcgtcatg gacaacggct gtcactttcg ggatagatga
      121 gattccggtt ctggatgtgt cggatatcag tgtgaaactc caggaggcat aacgtgcaga
      181 atgtggctgc cactgtgctt gcgcagtatg ctgccagccc ccgactcaat gccctcatta
      241 acagctttaa cgcagcgctt tcccccgaca gttttatcag tgatttttat ggtcttatct
      301 ggaacatcga taccgcagaa aagtatggtc ttgatgtctg gggaaagatt gtgggtgtca
      361 gtcgctggct gacggtaaag gacgatttta attacctggg cttcagcgag tccaggatgg
      421 acaccccggt aatggatgat ccctgtccgt ttaatcaggc accgttttac aacggaaaat
      481 cggatacccg gactgttgac ctgtctgatg ctgtataccg gcggctgata ctgatgaaag
      541 ccatgtcgaa cattactgac tgttccgttc cggatattaa cctgatgctg agatttatgt
      601 tcggaaaaaa acgccgggct tatgttctga ataatggcgg gctgaggatg agttacgtct
      661 ttgagtccgc gctctcgttg gcagaactgg cgattatcca gtcgtcgggt gcactgccat
      721 ccccgccggg tgtttatgtt tcagtagttt taaaggagtc ccgtaatgaa ggccagtgat
      781 aaaccccgcc agctggcggt cccctttgcg agtaccggag ataaaaaccg tatcccggac
      841 aaggcgacac agcagaccag agagagcggt aatgctgcgt atgattcagg ttttcctccg
      901 gtgaccatga cagcggtctc agcgggaggt ataccgccac acggcaagga ttttaacggt
      961 ctgatgtacg atattaccgc agcaata
//
LOCUS       DAJVPP010000267          981 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVPP010000267 DAJVPP010000000
VERSION     DAJVPP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.278"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..78
                     /gene="ydcY"
                     /locus_tag="OQM34_004173"
     CDS             <1..78
                     /gene="ydcY"
                     /locus_tag="OQM34_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 domain-containing protein"
                     /protein_id="HCS1454363.1"
                     /translation="AHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(79..528)
                     /locus_tag="OQM34_004174"
     CDS             complement(79..528)
                     /locus_tag="OQM34_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1454364.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(525..>981)
                     /gene="mddA"
                     /locus_tag="OQM34_004175"
     CDS             complement(525..>981)
                     /gene="mddA"
                     /locus_tag="OQM34_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCS1454365.1"
                     /translation="VLYTAAIWNDQTVDADNRIAWFEARTIAGYPVLVSEEDGVVTGY
                     ASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHVMVAGIESQ
                     NQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQLQLDERTEPDAIG"
BASE COUNT          276 a          290 c          226 g          189 t
ORIGIN      
        1 gctcatcacg gtcgcaagca taaagaagat atggaagcgc gccacgaaca gttaaccaaa
       61 ggcggcacca tcctctgatt aaaatgagcg tctggccacc aaccaggcgc caaccacaag
      121 taaaattgcg ccacatattg ggccgaacaa cgcccgtagt ggtacgccat gactgcggaa
      181 gatatccagc accagaccgc caatcagctg gctggcgacc agtaccgcaa tagttgtcgc
      241 cgctcccaca ttctgatatc cgctgatact ggcgaagaca aaaaacgaac ccagcaagcc
      301 aggtataagt gtccaccagc gcacgctgga caccagttcg ccaaaccccg ccatgccttg
      361 tttaaaccat aaaatagaaa caaagagcac aatccccacc agtgagttca gcaacatggc
      421 gatgagaatg gtagaggagg tctgggtgat gcgcgccatc agcgtgttct ggactaccag
      481 accaattccg gcggcaatca aaaaagcaag ggtgagcgac tgattcatcc aatcgcgtcc
      541 ggttcagtgc gctcgtcgag ttgcaactgc ataaatgtca gatccagcca atgaccaaat
      601 ttagtgccta cctgcggcat ttgtgcggtg acgacaaatc ccagcgactg gtgcagatgc
      661 agcgaagcct gattttgcga ttcgatcccg gcgaccatga catgcttccc gcaatcccgc
      721 gcttcatcaa tcaatcgact taacaattta cgccccagac ctttgccctg atgatcggga
      781 tggacataaa ccgaatgttc cacggtatgg cgaaaaccat cgaaactacg ccagtcgcca
      841 aacgaggcat atcccgtaac tacgccatct tcttcactta ccagcaccgg ataacctgct
      901 atggtccgcg cttcaaacca ggcaatgcgg ttatctgcat ccaccgtttg gtcattccag
      961 atagccgccg tatacaacac g
//
LOCUS       DAJVPP010000268          965 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVPP010000268 DAJVPP010000000
VERSION     DAJVPP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.279"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..483)
                     /locus_tag="OQM34_004176"
     CDS             complement(1..483)
                     /locus_tag="OQM34_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1454366.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="OQM34_004177"
     CDS             complement(474..776)
                     /locus_tag="OQM34_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1454367.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          293 a          235 c          195 g          242 t
ORIGIN      
        1 tcagacttta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctgggta caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaatacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgc
//
LOCUS       DAJVPP010000269          960 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVPP010000269 DAJVPP010000000
VERSION     DAJVPP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.280"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(31..90)
                     /locus_tag="OQM34_004178"
     CDS             complement(31..90)
                     /locus_tag="OQM34_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1454368.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(329..>960)
                     /locus_tag="OQM34_004179"
     CDS             complement(329..>960)
                     /locus_tag="OQM34_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454369.1"
                     /translation="SQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQD
                     AETKAVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQ
                     TSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLA
                     EQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          196 a          260 c          235 g          269 t
ORIGIN      
        1 gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta cgagtaacaa
       61 cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg ccccttcctc
      121 gggaggggct ttcccgtttc agccccttgc tgactccccg attgttgatg agcgccggac
      181 ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg caaaacgttg
      241 caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc cccggctttt
      301 tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg ggagttgttg
      361 agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt tcattgagca
      421 aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat gcttgttccg
      481 ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg ccactattca
      541 gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca acggattttt
      601 ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg gtttgttcat
      661 cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc gccagaccct
      721 ttttacgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct gcggcttctt
      781 tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc tgcgcggcag
      841 cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg gtttctacgt
      901 tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct gcctgggact
//
LOCUS       DAJVPP010000270          949 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVPP010000270 DAJVPP010000000
VERSION     DAJVPP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.281"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..628
                     /locus_tag="OQM34_004180"
     CDS             <1..628
                     /locus_tag="OQM34_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HCS1454370.1"
                     /translation="STVVATHLNHLISQHAAELFGRQEAQQLLDRVAQEMPKLTEDLV
                     PGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAPIQSDPHELTAVVRVALGRAI
                     TQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGLADRLLAQTQEALSRQEMLGA
                     PPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRHIRMTATIGGK"
     gene            628..>949
                     /gene="flhe"
                     /locus_tag="OQM34_004181"
     CDS             628..>949
                     /gene="flhe"
                     /locus_tag="OQM34_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCS1454371.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWE"
BASE COUNT          200 a          248 c          283 g          218 t
ORIGIN      
        1 cagcacggtg gtagcaacgc atcttaacca cctcattagc cagcatgccg cagagctgtt
       61 tggtcgtcag gaggcgcaac agctgttgga tcgcgtcgcc caggagatgc caaagctgac
      121 ggaagatctc gttcctggcg tcgtcacgct caccacactg cataaagtgc tgcaaaatct
      181 cctcgatgaa aaagtaccga ttcgcgatat gcgcaccatt ctcgaaacgc tggcggaaca
      241 tgcgcccatc caaagcgatc cacatgaatt aaccgccgtc gtgcgcgtgg cgttgggacg
      301 ggcgattacc cagcagtggt ttcctggcaa agatgaagtc catgttattg gcctcgatac
      361 accgctggaa cgtttgttac tacaggcgct gcagggcggg ggaggactgg agccagggct
      421 ggcggatcgt ttactggcgc aaactcagga agctctatcc cgtcaggaga tgctgggtgc
      481 gccgccagta ttgttggtga accacgcgct gcgaccatta ttgtctcgct tcctgcgccg
      541 cagcttgccg cagttagtgg tcctgtcgaa tctggaactg tctgataacc gacatatccg
      601 catgacggcg acaattggcg gtaaatgatg agaaccttat tagcgatatt attgtttccg
      661 ctgctggtgc aagccgccgg ggaggggatg tggcaggcaa gtagtgtcgg aattacgcta
      721 aatcatcgcg gtgagtcgat gtcgtctgca cctctttctg cgcgacaacc tgcttcagga
      781 ttgatgacgc tggtggcgtg gcgttatcag cttatcggcc cgacacctgc aggactgcgg
      841 gttcgcttgt gttcgcaatc tcgttgtgtc gaattagagg ggcagagcgg taccaccgtg
      901 gccttttccg gtatagcggc agcagaaccg ttgcgattta tctgggaag
//
LOCUS       DAJVPP010000271          943 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVPP010000271 DAJVPP010000000
VERSION     DAJVPP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.282"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..919)
                     /locus_tag="OQM34_004182"
                     /pseudo
     CDS             complement(1..919)
                     /locus_tag="OQM34_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247056.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          239 a          246 c          216 g          242 t
ORIGIN      
        1 ataatctctc cccaacgact gcgttagcgt tccctccacg cgactacgtc ggttacctgt
       61 ttcccaaaag tcttcagggc tgttgcgccg cgatgcccac tcattgagtg gtatagtagc
      121 cctcggtgga gtaacgatag ccaaccaggc gcaagctagt gtctgtgtcg tcaaatagtt
      181 ttgaatagag aaagcgatat gactgacccg tttctgtatc atcatggcta aaattcgcac
      241 gagcatgtgt cacatcgaaa gaaattgcgc ccaaagcgcc aagatcttta ccgacaccca
      301 gagctgcaga accataattt tcagcagcct gcatacctcc atataaagtg atcccgtggg
      361 aaaaaccatg aagtatttgc gcctgaagga cgtccggtgt aaatccgtct tcatcgcgca
      421 attcacccac gctgacatca acatctgtca gaccttcacg tttaagaatc gccaatgaag
      481 cataaggttg ggtgaatcgt tgttcgctac cgtcttcttc tttgatggtt acgtccaaat
      541 cgccacctgt acttgcggtg ttgagatcgg tgatctcaaa agcaccttgt ggaacggctg
      601 actgataaat cacgtagcca ttctgccgaa tgatgacggt ggcgttggac tttgcaatcc
      661 cacgaaccac aggagcaaag ccattctggc tggcgggtag catatcgtta tcagtataca
      721 aacgcgcgcc gcgaatttgc gtactgtcga aaatatcgct cgccgtccac gtatcaccaa
      781 tcataatctg gcttcttaac gcggcaatat cgcgctgtat ccaggtacta atggagttgt
      841 attccgcacg gccatcgctg gtgtttaaag tactgtaatt acgtagccgc cacggtccaa
      901 tattcagtcc attgcgcaga ttgagataac tggtatctgt ctc
//
LOCUS       DAJVPP010000272          924 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVPP010000272 DAJVPP010000000
VERSION     DAJVPP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.283"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..491)
                     /locus_tag="OQM34_004183"
     CDS             complement(<1..491)
                     /locus_tag="OQM34_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCS1454372.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAA"
     gene            complement(503..924)
                     /locus_tag="OQM34_004184"
                     /pseudo
     CDS             complement(503..924)
                     /locus_tag="OQM34_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          200 a          265 c          230 g          229 t
ORIGIN      
        1 gcagcgttgt tgacaggcac gccgtccagg gtaacaaggc ctttcaggtt taatgcggaa
       61 tcgccaacat atacctgttc atcgttatcc atctgccatt tcagttgcat accgtcatac
      121 ttctgcgtat caatcgggcg gccgacctgc tgagcagcct gcaattctat gaccgtccag
      181 ccaagttcca tcccccacag gttcagcggg ttaccggatt tgccgatatc cacgttcacg
      241 ccagcaatag cggttgagtc tttgcctacc cagttttttc cattcggatt tgcgccagta
      301 cccgcagcgg cgaagctggt attcgtccag ctggaaatgt catctgcgat ggaggcgtct
      361 tcacgcagtt gaatatcgcg gctccaggtg taccccacca gtggcaggtt cagcgtctgg
      421 tcgagtctct ccagctcccc gatgagaaag gcaccagagc tgtcaacggt tgcctgatca
      481 aaagtaatca ttcgtctgtt ccttaaatct tccaggaaat ttctgcattg ccgtcagcat
      541 caccggcacc tgtgaattca gcgttggtca gcaccacgtt tttgccactg actgacgtgg
      601 acatgaatcc acccagaggc actttgatgg attcatcagt ggagatcgcc ttttttgata
      661 gtgctggcat caaaatcaga accgagatta acggtcacgt agccacgctt catggcgtcg
      721 cccgggaagt tcttgcctgt tcccacctgg cgaaccatgt ccggctgcga cgtggtcgga
      781 taagggcgca cgtagatccc cttcaccttg tctgcggtat caccatctgc cagcggcacg
      841 aaaaaaccgt catcatcgta tttaccagcc aggccatagg cagcgaaggc gttatcggat
      901 ttaaggacca ccggttcgac ggtt
//
LOCUS       DAJVPP010000273          885 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVPP010000273 DAJVPP010000000
VERSION     DAJVPP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.284"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..490)
                     /gene="pdeC"
                     /locus_tag="OQM34_004185"
                     /pseudo
     CDS             complement(1..490)
                     /gene="pdeC"
                     /locus_tag="OQM34_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
BASE COUNT          278 a          191 c          183 g          233 t
ORIGIN      
        1 gacatcactt cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata
       61 taggtcattt tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta
      121 ggttcacgct tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat
      181 gagcataaaa aatggttatc acgaattcat taatataaag atagccacga ataatattca
      241 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
      301 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
      361 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
      421 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
      481 gatgactcat tgctatcaat ttcctgcctg tttatacccg gccattgcaa ggtgaaaccc
      541 tggctaccga cgtctttaga taaatcttac ggtataaata gcacttttgc ctgatttgaa
      601 attgatacct ttttaatcgt cacctggatg aaaataattc attgatattc aaaattaatt
      661 ttctacagga atacagaggc ggcgggaaga tatgccctta atgccactga aaaaccacat
      721 aatttgtaca gcaataaaag tcacggccta atatggctaa caaacaatca attgtgatat
      781 agttcacaaa attaatgaaa caaacagagt gtttcatttt tgtgttctgc aagccccgcg
      841 atagccagag tgtaccacgc ctcccgtgaa caacgccgcg ctgtc
//
LOCUS       DAJVPP010000274          861 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVPP010000274 DAJVPP010000000
VERSION     DAJVPP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.285"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..101)
                     /gene="rrf"
                     /locus_tag="OQM34_004186"
     rRNA            complement(<1..101)
                     /gene="rrf"
                     /locus_tag="OQM34_004186"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(197..>861)
                     /locus_tag="OQM34_004187"
     rRNA            complement(197..>861)
                     /locus_tag="OQM34_004187"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          181 a          275 c          196 g          209 t
ORIGIN      
        1 actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc
       61 ggcatggggt caggtgggac caccgcgcta cggccgccag gctaattctg ttttatcaga
      121 ccgcttctgc gttctgattt aatctgtatc aggctgaaaa tcttctctca atccgccaaa
      181 acatcttcgg cgttgtaagg ttaagcctca cggttcatta gtaccggtta gctcaacgca
      241 tcgctgcgct tacacacccg gcctatcaac gtcgtcgtct tcaacgttcc ttcaggaccc
      301 ttaaagggtc agggagaact catctcgggg caagtttcgt gcttagatgc tttcagcact
      361 tatctcttcc gcatttagct accgggcagt gccattggca tgacaacccg aacaccagtg
      421 atgcgtccac tccggtcctc tcgtactagg agcagccccc ctcagttctc cagcgcccac
      481 ggcagatagg gaccgaactg tctcacgacg ttctaaaccc agctcgcgta ccactttaaa
      541 tggcgaacag ccataccctt gggacctact tcagccccag gatgtgatga gccgacatcg
      601 aggtgccaaa caccgccgtc gatatgaact cttgggcggt atcagcctgt tatccccgga
      661 gtacctttta tccgttgagc gatggccctt ccattcagaa ccaccggatc actatgacct
      721 gctttcgcac ctgctcgcgc cgtcacgctc gcagtcaagc tggcttatgc cattgcacta
      781 acctcctgat gtccgaccag gattagccaa ccttcgtgct cctccgttac tctttaggag
      841 gagaccgccc cagtcaaact a
//
LOCUS       DAJVPP010000275          850 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVPP010000275 DAJVPP010000000
VERSION     DAJVPP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.286"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..680)
                     /locus_tag="OQM34_004188"
                     /pseudo
     CDS             complement(1..680)
                     /locus_tag="OQM34_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(728..>850)
                     /locus_tag="OQM34_004189"
     CDS             complement(728..>850)
                     /locus_tag="OQM34_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1454373.1"
                     /translation="AIDAEGKAEMKFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          233 a          188 c          177 g          252 t
ORIGIN      
        1 cgccaggtca tttggcctcc cgcagaatta gcaggtaaag gtaacgtgag cgtttcaaaa
       61 ggacggatca tattgagggg cacatcatgg ccatcaacga tgacatccat cacactgata
      121 tgaattgggt tgggattctc tgctatcaga tttcgccctt cccggcgcca tttcactttc
      181 ggcgcagcat cttcgggaat aaggcggata tttgctggtc gccagaacag cttgatacgc
      241 gtttttactg ctatttccag cgagttcgtc gctttgttgc tcgctgatgg cggaatattt
      301 ttaatattca gccagaagac tgactcgcga tcatcaggca atgacacacc tttggtgtga
      361 ataatgcgta atattttttc ttgcgaaggt tctaatcgtg atactggcgg aataacggta
      421 aacggcgctt tgcttttatc ctctttatta aatggatcta cccaagcctg gatgagataa
      481 ggtatttttt cttctttgct aaatacagaa atagatttat cagcattatt acttaagtaa
      541 accacccgcg tactaccaat gacaacgcct gcataagtta atgctggttg gcctaaaaaa
      601 acgctgagta taatcgcggt aaataactta cattaaactg ttgttttaaa gagcctgatt
      661 aaacaacttt ttctggacat aacaactccc tggccaaagc caactgaata aaaaggccct
      721 tgataaatta tttcttatat tcgacagtaa attgtgaatc agcgttcgcc gtaccagccg
      781 ttacggtcgc attggttgcc acatagcgtg caataaactt catttcagcc ttgccttcag
      841 catcgatagc
//
LOCUS       DAJVPP010000276          847 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVPP010000276 DAJVPP010000000
VERSION     DAJVPP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.287"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..414
                     /locus_tag="OQM34_004190"
     CDS             <1..414
                     /locus_tag="OQM34_004190"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="FAD-binding subunit; with XdhA and XdhC
                     participates in purine salvage; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS1454374.1"
                     /translation="GKVSLEHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGC
                     AAHCRLDNGNFSELRLAFGVAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAP
                     RSSWRASKEFRLHLIQTMTKKVISEAVAAAGGKLQ"
     gene            411..>847
                     /gene="xdhC"
                     /locus_tag="OQM34_004191"
     CDS             411..>847
                     /gene="xdhC"
                     /locus_tag="OQM34_004191"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS1454375.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGY"
BASE COUNT          215 a          225 c          230 g          177 t
ORIGIN      
        1 ggcaaagtgt ctcttgagca tgacgaaatc ctcgtcgcct ttcattttcc gccacagccg
       61 aaagaacacg cgggcagcgc gcattttaaa tatgccatgc gcgacgcaat ggatatttca
      121 acgattggct gcgccgcaca ttgccgactg gataacggca atttcagcga attacgcctg
      181 gcatttggtg ttgccgcgcc aacgccgatt cgctgccaac atgccgaaca gactgcacaa
      241 aatgcgccat taaacctgca aacgctggaa gctatcagcg aatctgtcct gcaagatgtc
      301 gccccgcgtt cttcatggcg ggccagtaaa gagtttcgtc tgcatctcat ccagacgatg
      361 accaaaaaag tgattagcga agccgtcgcc gcggcggggg gaaaattgca atgaatcaca
      421 gcgaaacaat taccaccgaa tgcaccatta acgggatgcc ttttcagctt cacgccgcac
      481 caggcacgcc gctctcggaa ttactccgcg aacaaggact gctaagtgtc aaacaagggt
      541 gctgcgtggg tgaatgtggt gcctgtacgg tgttggtcga cggcacagca atagacagtt
      601 gcttatacct tgccgcctgg gctgaaggaa aagagatccg cacgctggaa ggtgaagcga
      661 aaggcggaaa actttctcat gttcagcagg cttatgcgaa atccggcaca gtgcagtgcg
      721 ggttttgtac gcctggcctg attatggcta ccacggcaat gctggcgaaa ccacgcgaga
      781 agccattaac cattacggaa attcgtcgcg gactggcggg aaatctttgt cgctgcacgg
      841 ggtatca
//
LOCUS       DAJVPP010000277          835 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVPP010000277 DAJVPP010000000
VERSION     DAJVPP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.288"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..441
                     /locus_tag="OQM34_004192"
     CDS             <1..441
                     /locus_tag="OQM34_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1454376.1"
                     /translation="GSNSFTFVMFSCVAFFGTAFVNSLNWALVPDTVDYGEWKTGIRA
                     EGSVYTGYTFFRKISAALAGFLPGIMLTQIGYVPNIAQSDATLQGLRQLIFIWPCALA
                     IIAALTMGFFYTLNEKRFALIIEEINQRKNKEMATEKKTASVTL"
     gene            509..>835
                     /gene="ompL"
                     /locus_tag="OQM34_004193"
     CDS             509..>835
                     /gene="ompL"
                     /locus_tag="OQM34_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCS1454377.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT          227 a          173 c          186 g          249 t
ORIGIN      
        1 ggaagtaact ctttcacttt cgtcatgttc tcttgtgtcg ccttttttgg cacggccttt
       61 gtaaacagcc tgaactgggc actggtacca gataccgtag attacggtga atggaaaacc
      121 ggtattcgtg ccgaaggttc tgtttatacc ggttatacct tctttcgtaa aatttctgcc
      181 gcacttgctg gcttcttgcc aggcattatg ctgacgcaaa ttggttatgt tcccaacatc
      241 gcgcaaagcg atgcgacatt acagggtttg cgtcagctca tttttatctg gccttgtgca
      301 ctggcaatta ttgctgcatt gacgatggga ttcttttaca cactcaatga aaaacggttt
      361 gcattgatta ttgaggaaat caaccaacgc aaaaataaag agatggcgac agaaaaaaaa
      421 acggcttccg taacgttata aatgtcaata gccgctattt ccatcctggt gggtggcggc
      481 ctccctacgt ttaaaaaatg gacttattat gaaaaagatt aatgcaataa ttttattatc
      541 ttctctgacc tctgcctcgg tatttgctgg cgcatacgtc gaaaacaggg aggcatataa
      601 tcttgcctcc gatcagggcg aggttatgct acgtgtgggt tataacttcg atatgggcgc
      661 gggtattatg ttaaccaata cctacacctt tcagcgagaa gatgaactaa aacatggcta
      721 taatgaaatt gaaggttggt atcctttatt caaaccaacc gataaattaa ccatccagcc
      781 cggtggctta attaatgata agagtatcag ttcgggtggt gcagtgtatc tggac
//
LOCUS       DAJVPP010000278          811 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVPP010000278 DAJVPP010000000
VERSION     DAJVPP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.289"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>811
                     /locus_tag="OQM34_004194"
     CDS             <1..>811
                     /locus_tag="OQM34_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1454378.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          259 a          187 c          122 g          243 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DAJVPP010000279          804 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVPP010000279 DAJVPP010000000
VERSION     DAJVPP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.290"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            158..>804
                     /locus_tag="OQM34_004195"
     CDS             158..>804
                     /locus_tag="OQM34_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1454379.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAE"
BASE COUNT          245 a          135 c          167 g          257 t
ORIGIN      
        1 gtgtgccacc tcaaggttct tagtgagccg tttttaaaag atatgataat atattattgt
       61 ttataaattg ttgatgtttg atttagtagt atataaagtt caccaccttt attaggcttt
      121 ccttttatct ctacttactg ggtgccagaa actttaaatt ttaaacggag ggggcatttt
      181 gtttaaagca aatcatgttc cggtattaat gtatcatcat gtcagccatt gcccaggact
      241 ggttacactt tctcccgaga cattccggaa acaaatgaaa tggttggctg aaaacaactg
      301 gaaaacactg tcatcagatg aactggagtt tttttatcgg ggcggaaaac ttccccgtaa
      361 aagcgttatg ttgacatttg atgatggata ccttgacaac tggtttcagg tatatccttt
      421 attaaacgag tttaatctca aggcacatat ttttttaatt accagtttta ttggaaatgg
      481 tccggttcgt cactctcctg gaaaggaata ttctcatcgc gattgcgaac atcaaatagc
      541 aaccgggaat gctgataatg ttatgcttcg ctggtctgaa gtgaacgaaa tgttgcaatc
      601 tggtctggtc gaatttcatg ttcatacaca tactcataca cgttgggata aaaagttcac
      661 ttcacgagaa gaacagtgta aacatcttcg gcaggactta ttgagcggga gagaatattt
      721 aaaaaaaatg acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt attataacaa
      781 ggattatatt cagatcgctg aaga
//
LOCUS       DAJVPP010000280          793 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVPP010000280 DAJVPP010000000
VERSION     DAJVPP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.291"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..517
                     /locus_tag="OQM34_004196"
     CDS             <1..517
                     /locus_tag="OQM34_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1454380.1"
                     /translation="SHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDAGYV
                     TPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPYFKL
                     AYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQD
                     WSANVGVKYTW"
     gene            605..>793
                     /locus_tag="OQM34_004197"
     CDS             605..>793
                     /locus_tag="OQM34_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1454381.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIG"
BASE COUNT          214 a          173 c          187 g          219 t
ORIGIN      
        1 ctctcacttc aacaacgacc tgtctgcaac catgagcaac ggtacttacg ttgacggtag
       61 caccaactcc gacgcttggg gcttcggttt gaaaaccggt tacgacttca aactgggtga
      121 tgctggttac gtgactcctt acggcagcat ttctggtctg ttccagtctg gtgatgacta
      181 ccagctgagc aacgacatga aagttgacgg tcagccttac gacagcatgc gttatgaact
      241 gggtgtagat gcaggttata ccttcaccta cagcgaagat caggctctga ctccgtactt
      301 caaactggct tacgtctacg acgactctaa caacgataac gatgtgaacg gtgattccat
      361 cgataacggt actgaagggt ctgcggtacg tgttggtctg ggtactcagt tcagcttcac
      421 caagaacttc agcgcctata ccgatgctaa ctacctcggt ggtggtgacg tagatcaaga
      481 ctggtccgcg aacgtgggtg ttaaatatac ttggtaatat tcttcactcc gaagaaatac
      541 tggtaattta atctaaataa tgcccgtcaa ggatttgacg ggcattactg caaaggacgc
      601 gcaaatgtta tctgtagtta aacctcttca ggaatttggt aagctcgata aatgtttgtc
      661 cagatacggt acgcgcttcg agtttaataa tgaaaagcaa gttatatttt ccagtgatgt
      721 caataacgaa gatactttcg ttattttaga aggagttatc tctctgcgta gagaagaaaa
      781 cgtacttatc ggt
//
LOCUS       DAJVPP010000281          789 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVPP010000281 DAJVPP010000000
VERSION     DAJVPP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.292"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..595
                     /locus_tag="OQM34_004198"
     CDS             <1..595
                     /locus_tag="OQM34_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454382.1"
                     /translation="ASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLA
                     SNSPANRILLIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFA
                     YTLILHRTVYLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENN
                     DLPLDAICNAIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(599..>789)
                     /locus_tag="OQM34_004199"
     CDS             complement(599..>789)
                     /locus_tag="OQM34_004199"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="catalyzes the formation of
                     (E)-3-(methoxycarbonyl)pent-2-enedioate and
                     S-adenosyl-L-homocysteine from S-adenosyl-L-methionine and
                     trans-aconitate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCS1454383.1"
                     /translation="QAIIDWVTATGLRPWLQDLTESEQQLFLTRYHQMLEEQYPLQEN
                     GQILLAFPRLFIVARRTE"
BASE COUNT          186 a          198 c          186 g          219 t
ORIGIN      
        1 ggcaagtgta agggagtttg ccaggctgca aatcgccttc gcccactgtt tacgcatgac
       61 attacgcaaa cagccacagg cggaagtgct ggctcattat ctcaagactg gagatcttca
      121 gcgtgtactg gcttcgaact ctccagctaa ccgtatcttg ttaataatgg gagaatggtt
      181 ggcggttcag cgccgcaatg gacagctttc agatatcctg tttattagcc tcaacgatcg
      241 gcttaatgat atttcagcgg ttctggcagg ctgtgagcgc attgcctaca cgccaattcc
      301 ctttgcctac accctgattt tgcatcgtac cgtttatctg ttttgtatca tgctgccgtt
      361 cgcgctggtc gtggacctgc attacatgac gccttttatc tctgtgctga tttcctacac
      421 ttttatttcg ctggattgtc tggcggaaga actggaagat ccgttcggta ctgaaaacaa
      481 tgatttaccg ctggatgcca tctgcaacgc tattgaaatt gacctgttac agatgaacga
      541 tgaagcagaa attccagcaa aaattcttcc cgatcgtcat taccagctga cgtgataatt
      601 actccgtacg gcgggcaaca ataaacagac gcggaaatgc cagcagtatc tgtccgttct
      661 cttgcagtgg atactgctct tccagcatct gatggtagcg cgtaagaaaa agctgctgtt
      721 cgctctcggt cagatcctgt aaccacggac gtaatccagt ggcggtcacc caatcgataa
      781 tcgcctggt
//
LOCUS       DAJVPP010000282          781 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVPP010000282 DAJVPP010000000
VERSION     DAJVPP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.293"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>781)
                     /locus_tag="OQM34_004200"
     CDS             complement(<1..>781)
                     /locus_tag="OQM34_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1454384.1"
                     /translation="LFTAIGKRNIAELKTRDLLVPIKAVESSGRLEVAARLQQRTTAI
                     MRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAV
                     ELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRGSKMRTPHLVPLS
                     RQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYDTKVEVCGHGFRT
                     MACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGE"
BASE COUNT          187 a          229 c          177 g          188 t
ORIGIN      
        1 gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta cgctcctggt
       61 gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc aacgaactac
      121 aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac cccattacgc
      181 gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga tcgcccacaa
      241 aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt agggcctgcc
      301 gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa tgcttgactc
      361 cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa tctatttctg
      421 accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg agttcaaccg
      481 ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat tcaggtatac
      541 ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca cctgcaatct
      601 cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata atcgcggtag
      661 tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact gctttaatgg
      721 gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata gcagtaaaga
      781 g
//
LOCUS       DAJVPP010000283          770 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVPP010000283 DAJVPP010000000
VERSION     DAJVPP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.294"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..211
                     /locus_tag="OQM34_004201"
     CDS             <1..211
                     /locus_tag="OQM34_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1454385.1"
                     /translation="TAPDATKASGVSVTLMRNGKILATGENVSLGTVNKSKVPLGLSA
                     TYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            545..>770
                     /locus_tag="OQM34_004202"
     CDS             545..>770
                     /locus_tag="OQM34_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454386.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAW"
BASE COUNT          242 a          143 c          167 g          218 t
ORIGIN      
        1 cacagcacct gatgcaacaa aagcatcggg agtgagtgtc actctaatgc gtaatggaaa
       61 aatactggct acgggagaaa atgtctccct ggggacagta aataaaagca aagtacctct
      121 gggtttaagc gcaacctatg gacaaacagg aaacaaggtt tcagccggaa ccgtccagtc
      181 agtcattggt gtcactttta tctatgaata attgtgattt tgaccttagt ctatgcgctc
      241 cattcacgaa aattgcgcgg ataattattt tgtgaaggct attatcctac acctgtaaga
      301 aaatccgcgc acaattgaag ccagagtctt attttttata ttgaaaaata tgttaattta
      361 aacactatga gagatgatgc ttaacttatc gtatttaatc tattgtttaa cgataataga
      421 ttaaaaatag aaagagagta gtcccttact cttatatatc catgttggcg ataatccctt
      481 tgtatgtact gtgcatcatc gctattacaa atcctaataa ttcatttcca cacaggataa
      541 gtagatgaag aaaaaaattg aatcctacca gggtgctgca ggtggttggg gtgctgttaa
      601 atccgtagcg aatgcagtac gtaagcagat ggatatacgc catgatgtta ttgccatgtt
      661 tgacatgaat aagccagagg gctttgactg tccgggttgt gcatggccag atcctaagca
      721 cagtgcgtca ttcgacattt gtgaaaacgg cgcaaaagca atcgcctggg
//
LOCUS       DAJVPP010000284          770 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVPP010000284 DAJVPP010000000
VERSION     DAJVPP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.295"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>770
                     /locus_tag="OQM34_004203"
     CDS             <1..>770
                     /locus_tag="OQM34_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1454387.1"
                     /translation="DDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTS
                     EQIYMLSLSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYN
                     SHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGET
                     IALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNT
                     VVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPF"
BASE COUNT          207 a          162 c          202 g          199 t
ORIGIN      
        1 ccgatgatgt cgaacgatta atgcaatttg gctacagcag tagctggaag cgtctctcgt
       61 ggaacgtctc ctggagttat tccaatactg ccagacaggg gacggggaac aaccatgcca
      121 gtgataacac cagtgagcag atctacatgc tctctttatc tgttccttta tcgggctggt
      181 ggggtaatag ttacgccacc tattctgttt cgcaaaacga taattccggt agctcacatc
      241 aactcggact cagcggtacg gcgctggaaa gaaataacct ttcatggaat ttaatgcagt
      301 cctataacag tcatgatgat gaggttggcg gtaatatgtc cctgacctat gatggctctt
      361 atggcacggt gaacggcagc tataactaca gccaaaattc ccagaggctg aattatggta
      421 tcagaggggg aattctggca cacagcgaag gggtaacgtt aagtcaggag ttaggtgaaa
      481 ctattgctct tgttaaagca cctggggccg ccgggttaga aatagataat atgcgcggtg
      541 ctgcgacgga ctggcgcggc tatacggtca agacacagct aaacccttat gatgaaaatc
      601 gggtagcaat cagcgataac tatttctcga agtcgaatat agaacttgat aataccgtcg
      661 ttacgatggt tcccacgcgt ggtgcagtgg ttaaagcgga gtttgtgact catgtgggtt
      721 atcgcgttct cttcagagtg ttaaatgcaa atggtaaacc ggtacctttt
//
LOCUS       DAJVPP010000285          759 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVPP010000285 DAJVPP010000000
VERSION     DAJVPP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.296"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..751
                     /gene="ssuA"
                     /locus_tag="OQM34_004204"
     CDS             <1..751
                     /gene="ssuA"
                     /locus_tag="OQM34_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCS1454388.1"
                     /translation="EALNVGSIDLGSTGDIPPIFAQAAGADLVYVGVEPPKPKAEVIL
                     VAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQPTYLTPADARAAF
                     QQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPYAEKNGAFIQGVL
                     ATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKPVNAEVAALQQQT
                     ADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
BASE COUNT          188 a          212 c          191 g          168 t
ORIGIN      
        1 ggaagcgtta aacgttggca gtattgatct cggcagtacc ggggatattc cgccaatctt
       61 tgcccaggct gccggggctg atttggtata tgtgggcgtc gagccaccga agcccaaagc
      121 cgaagtgatt ctggtggcag aaaacagccc gatcaaaacc gtagccgatc ttaaaggtca
      181 caaagttgcc tttcagaaag gttccagttc acacaacctt ttactgcgtg cactgcgtca
      241 ggccggactt aaattcaccg atatccagcc cacttacctg acgcctgccg atgcccgcgc
      301 cgcgttccag caaggtaacg ttgacgcctg ggctatctgg gatccctact actccgctgc
      361 gttattacag ggcggcgtgc gggtgctgaa agacggcacc gatctcaatc aaactggatc
      421 gttttatctg gcagctcgtc cctatgcaga aaaaaacggc gcttttattc agggcgtact
      481 ggcaaccttt agtgaggccg atgcgttaac ccgcagccag cgcgaacaaa gcatcgcttt
      541 actggcaaaa acgatgggct taccggcacc ggtgattgcc tcttacttag atcatcgccc
      601 tcctaccacc atcaaaccgg ttaacgccga ggttgccgcc ttacagcagc aaacggcaga
      661 tctgttttat gaaaaccgtc tggtgccgaa aaaagtcgat attcgccagc gcatctggca
      721 gcccactcaa ctggaaggaa aacaattatg agtctgaat
//
LOCUS       DAJVPP010000286          756 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVPP010000286 DAJVPP010000000
VERSION     DAJVPP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.297"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>756)
                     /gene="treF"
                     /locus_tag="OQM34_004205"
     CDS             complement(<1..>756)
                     /gene="treF"
                     /locus_tag="OQM34_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCS1454389.1"
                     /translation="LDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRD
                     TPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFI
                     PIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYD
                     WRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKP
                     NGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTV"
BASE COUNT          159 a          220 c          208 g          169 t
ORIGIN      
        1 accgttttca gccagctgcg cgcgatttca tcacccagaa ggtcatcgcc atacatttta
       61 aatccctgaa ttgccatcca ttgtaacggt gcccagccgt tgggtttatc ccactgttca
      121 ccggtttcgt actcgcttgc caaaatcccg ccaggtgtca gtaaccggct gcgtacggcg
      181 tttgccagac gatcggcctg ttcatggttc gccatgccga catagagcgg cacaatggcg
      241 gcagcggaaa acagcgccag ttgttcgcgt cgccagtcgt aatcgcggta gatgccgttt
      301 tcatcatccc agaggtaacg gtttaccgca tcgcgacggg cactggcctt ctggcggaac
      361 agcgcttctg tctctttctc gcctttcagc gccgagatgt tggcgatggc gctctccagt
      421 ttaaacagga aggcattcag atcgatgggg atgaactggg tggtacgaat gctcgccaga
      481 cgaccagtat cacgcagcca acgggaagag taatcccaac cggaggccgc tcccgcgcgt
      541 aaatcgcggt acacctcgtt gggcgggcga ccagaatgtt tcgcggtttc aacgtcctca
      601 agccaggatt cgtcacgcgg cgtgtcgcga tcgtcccagt aacggttgag cagcgatccg
      661 tccggcatcc gcacaacatg gcgataggcc tgattaggga tcaacgattc tgcaccgtcc
      721 atccagaagg catattccat tttaaggtgg tcgaga
//
LOCUS       DAJVPP010000287          745 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVPP010000287 DAJVPP010000000
VERSION     DAJVPP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.298"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..289)
                     /locus_tag="OQM34_004206"
     CDS             complement(<1..289)
                     /locus_tag="OQM34_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1454390.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPL"
     gene            complement(286..720)
                     /locus_tag="OQM34_004207"
     CDS             complement(286..720)
                     /locus_tag="OQM34_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1454391.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDVLGRCHEECPVEEGI"
BASE COUNT          167 a          228 c          177 g          173 t
ORIGIN      
        1 taagcggcgc ggttacggtg gtcggtaacc aactgccgac accttcgcgt gccttgtgca
       61 ttgccagctt gtcgagtaca tccggcgcgt catccattac atcaacgccc gcaccaccgt
      121 gtacatgggt gtcgatgtaa gcggggcaga gcagttccgc atcgcgttca gtcacgccca
      181 ctggaatcgg ttcgattgct gcgatgacac cgtcagcaac acgcaactga tggtcatcga
      241 accatccctc ttcagtcagc agccttctgg cgcgcagaac gtgtgtcata ttccttcctc
      301 gaccggacat tcttcatggc agcggcctaa cacgtccacc agactggtca gccctcggtg
      361 tccacactcc agcgcctgta cacggaattc ttcaccgctt aacccttcgc gctccagcac
      421 catctccagt agcagttgca aattggtgcc ggtaatcact tcacagcccg gtttttgcat
      481 cgccagcgtt gaagctacgc gaaacggcgt gccgcccagc aaatccgtca gaaaaacaat
      541 gccgtcttca caatccagtt gggcgatggc ctcttcaagc tgcgatgtaa gcagcgcggc
      601 gcttgaggtt tcgggaaaat cgatggcaat aaactgcgat tgatcgccga ggatttgctt
      661 cattgctttt tccatgccac tggcaaaccc gccatgccct gtcaaaataa tacttaacat
      721 ctaatacctc attgtattta aagga
//
LOCUS       DAJVPP010000288          725 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVPP010000288 DAJVPP010000000
VERSION     DAJVPP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.299"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..725)
                     /locus_tag="OQM34_004208"
                     /pseudo
     CDS             complement(1..725)
                     /locus_tag="OQM34_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
BASE COUNT          172 a          230 c          172 g          151 t
ORIGIN      
        1 caggtaacgt cccggcagcg gttccagggt taatcgccag ccagcgcccc ggataagcat
       61 caccactgcc aatctccacg cctttcatca aaatgcgata gcgggcaggt tgcagatcca
      121 tattgtcgcg aatgtgcacc actggcggca gaaatcccat ctcctgggca aatttcttgc
      181 ggatactgcg tatacggccc aacaactcac catcctgctg gaaatcgacc atcgggatca
      241 gtcgataacc cacttccatt cccagagaat cttccagttg tacatcgttc cacgtcgctt
      301 cgacaacggt attattctct gccattttta ccggtttggg ttcggcaggc gctttttgtt
      361 cgcgtccgcg tatccaccag gccagcccca gcaatccggc agtgaacagc aaaaatacca
      421 ggttcggcat tccaggcacc aggccgagta aaccgagcac ggcggcgctt aacaacataa
      481 cgcttgggtt actgaaaagc tgattcacca tctgctcgcc aacatcctga tcggtgctga
      541 cacgcgtaac gatgcccccc gcggcggtag aaatcaccag cgccggaatt tgtgccacca
      601 gaccgtcgcc aatggtcaat agcgtataac tttccgccgc gtgtcccatg ctcatgccag
      661 ttgcagcacg ccgaccagca acccgccgac aacgttaatg accatgatga ggatcccggc
      721 aatgg
//
LOCUS       DAJVPP010000289          723 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVPP010000289 DAJVPP010000000
VERSION     DAJVPP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.300"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            9..>723
                     /locus_tag="OQM34_004209"
     CDS             9..>723
                     /locus_tag="OQM34_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCS1454392.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGA"
BASE COUNT          189 a          175 c          183 g          176 t
ORIGIN      
        1 tattatttat ggctataagc gacacaaaac tgcgtactat ttatggtaaa ccatattcgg
       61 gcccacaaga agtggctgat gccgatggcc tcagcgtacg aatttcaccg aagggggtca
      121 tccagttcca gtaccgctat cgttggcatg gcaagcctaa tcgacttggg cttggtcgat
      181 acccatccct gtctttgaag gatgccagac agatcactgc tgacttgcga aagctctatt
      241 tctcaggaac ggatccacgc acctattttg aagagaaggt ggagaactcc atgacggtcg
      301 cccagtgtct cgactactgg ttcgacaact acgtctctac aactctcaga gaaaagaccc
      361 aggcacttta tcgatcaacg gttatgaagc gcatgcatga cgccttccct aatcgtccgg
      421 catcttctat cacggttaag caatgggttg acctgcttac cgaagaagaa aaagataatc
      481 cacgccgagc aaggcaggtg ctaagtcaac taagatcagc aattagttgg tgcatgcggc
      541 gtcagttgat agatagttgc gcaattatga gcatccagcc aagggacttc ggctcccgcg
      601 ctgaggtagg ggatcgggta ctgtcgtatc acgaactggc taagatttgg cttgctattg
      661 aaagaagccg tgcgtctacg tcaaataagc tccttcatca gatgcttatg ctgtgggggg
      721 cga
//
LOCUS       DAJVPP010000290          722 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVPP010000290 DAJVPP010000000
VERSION     DAJVPP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.301"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>722
                     /locus_tag="OQM34_004210"
     CDS             <1..>722
                     /locus_tag="OQM34_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1454393.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGG"
BASE COUNT          170 a          172 c          214 g          166 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg tggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaagggcgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 gg
//
LOCUS       DAJVPP010000291          714 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVPP010000291 DAJVPP010000000
VERSION     DAJVPP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.302"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..335
                     /locus_tag="OQM34_004211"
                     /pseudo
     CDS             1..335
                     /locus_tag="OQM34_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            328..714
                     /locus_tag="OQM34_004212"
                     /pseudo
     CDS             328..714
                     /locus_tag="OQM34_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
BASE COUNT          136 a          185 c          210 g          183 t
ORIGIN      
        1 tattcggtag cgttgcagta tgacgaaaac aaaatgagcg caccgaaagt ggtcgctaaa
       61 ggtgcagggc tggtcgcgct gcgcattcgt gaaattggcg ctgaaaataa cgtcccgacg
      121 cttgaagcgc cgccgctggc gcgagcgctg tatcgacatg cggagattgg tcaacaaatc
      181 ccgggtcaac tgtacgccgc ggtggcggaa gtgctggcct gggtctggca actgaaacgc
      241 tggcgtctgg ctggtggaca gcgccctgta cacctactca tcttccggtg ccggcagccc
      301 tggattttat taacgagaaa ccgacccatg agtaatctgg ccgcgatgct gcgcctgccc
      361 gcaaacctga aatcgacata atggcagatc cttgccggac cgattttgat cctgttgatc
      421 ttgtcgatga tggtgctgcc actgcccgca ttcatactcg acctgttgtt taccttcaat
      481 attgccttgt cgatcatggt gttgctggtg gcgatgttta cccagcgcac gcttgagttt
      541 gctgcgtttc cgaccattct gttgtttact acgttgttgc gtctggcact taacgtggct
      601 tcaacccgta tcattttgat ggaagggcat accggcgcgg cggcggctgg gaaggtggtc
      661 gaagcgttcg gtcacttcct cgttggtggc aatttcgcta tcggtatcgt ggtg
//
LOCUS       DAJVPP010000292          706 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVPP010000292 DAJVPP010000000
VERSION     DAJVPP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.303"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>706
                     /locus_tag="OQM34_004213"
     CDS             <1..>706
                     /locus_tag="OQM34_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS1454394.1"
                     /translation="AEFTQVWRRLLLGETVNFNGKHIHVRGAKLLFPPIQQPYPPLYF
                     GGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAYGRKIRFGIRLHVIVRE
                     TNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAALHNGKRDNLEISPNLWA
                     GVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPHLEEAYRVGELLFPHLD
                     VAIPEIPQPQPLNPQGE"
BASE COUNT          156 a          192 c          210 g          148 t
ORIGIN      
        1 gcggaattta cccaggtctg gcggcgctta ttgctggggg aaaccgtcaa tttcaacggt
       61 aaacatattc atgtgcgcgg agcaaaactg ctcttcccgc cgattcaaca gccttatcct
      121 ccgctttact ttggcggatc gtcagatgtc gcccaggagc tggcggcaga acaagttgat
      181 ctctacctca cctggggcga accgccggaa cttgttaaag agaaaatcga acaagtgcgg
      241 gcgaaagctg ccgcgtatgg gcgcaaaatt cgtttcggta ttcgtctgca tgtgattgtt
      301 cgtgaaacta acgacgaagc gtggcaggcc gccgagcggt taatctcgca tcttgatgat
      361 gagactatcg ccaaagcaca agccgcattc gcccggacgg attccgtagg acaacagcga
      421 atggcggcgc tacataacgg caagcgcgac aatctggaga tcagccccaa tttatgggcg
      481 ggcgttggct tagtgcgcgg cggtgccgga acggcgctgg tgggcgatgg tcctacggtc
      541 gccgcgcgaa tcaacgaata cgccgcgctt ggcatcgaca gctttgtgct ttcgggctac
      601 ccgcatctgg aagaagcgta tcgggttggc gagttgctgt tcccacatct ggatgtcgcc
      661 attccggaaa ttccccagcc gcagccgctg aatccgcaag gcgaag
//
LOCUS       DAJVPP010000293          684 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVPP010000293 DAJVPP010000000
VERSION     DAJVPP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.304"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..662
                     /locus_tag="OQM34_004214"
     CDS             <1..662
                     /locus_tag="OQM34_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1454395.1"
                     /translation="EKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT          160 a          152 c          196 g          176 t
ORIGIN      
        1 tggagaaaca ctcggtgtct cgtctggttg gtgcgcctcc gggatatgtc ggttatgaag
       61 aaggtggcta cctgaccgaa gctgtgcgtc gtcgtccgta ttccgtcatc ctgctggatg
      121 aagtggaaaa agcgcatccg gatgtcttca atattctgtt gcaggtactg gatgatgggc
      181 gtctgactga cgggcagggg agaacggtcg acttccgtaa tacggttgtc attatgacct
      241 ctaacctcgg ttccgatctg attcaggaac gcttcggtga actggattat gcgcacatga
      301 aagagctggt gctcggtgtg gttagccata acttccgtcc ggaattcatt aaccgtattg
      361 atgaagtggt ggtcttccat ccgctgggtg aacagcacat tgcctcgatt gcgcagattc
      421 agttgaaacg tctgtacaaa cgtctggaag aacgtggtta tgaaatccac atttctgacg
      481 aggcgctgaa actgctgagc gagaacggtt acgatccagt ctatggtgca cgtcctttga
      541 aacgtgctat ccagcagcag atcgaaaacc cgctggcaca gcaaatactg tctggtgaat
      601 tggttccggg taaagtgatt cgcctggaag ttaatgaaga ccggattgtc gctgtccagt
      661 aatgataaaa cgagcccttc gggg
//
LOCUS       DAJVPP010000294          683 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVPP010000294 DAJVPP010000000
VERSION     DAJVPP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.305"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..649
                     /gene="bamD"
                     /locus_tag="OQM34_004215"
     CDS             <1..649
                     /gene="bamD"
                     /locus_tag="OQM34_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1454396.1"
                     /translation="PPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLD
                     LIYAYYKNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDR
                     SDRDPQHARAAFSDFSKLVRGYPNSQYTTDATKRLVFLKDRLAKYEYSVAEYYTERGA
                     WVAVVNRIEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAANSSNT"
BASE COUNT          188 a          178 c          170 g          147 t
ORIGIN      
        1 tccgccaaat gaaatttacg cgactgcaca acaaaagctg caggacggta actggagaca
       61 ggcaataacg caactggaag cgttagataa tcgctatccg tttggtccgt attcgcagca
      121 ggtgcagctg gatctcatct acgcctacta taaaaacgcc gatttgccgt tagcgcaggc
      181 tgccatcgat cgttttattc gccttaaccc gacccatccg aatatcgatt atgtcatgta
      241 catgcgtggc ctgaccaata tggcgctgga tgacagtgcg ctgcaagggt tctttggcgt
      301 tgaccgtagc gatcgcgatc ctcaacatgc acgagctgcg tttagtgact tttccaaact
      361 ggtgcgcggc tatccgaaca gtcagtacac taccgatgcc accaaacgtc tggtattcct
      421 gaaagatcgt ctggcgaaat atgaatactc cgtggccgag tactatacag aacgtggcgc
      481 atgggttgcc gtcgttaacc gcatagaagg catgttgcgc gactacccgg atacccaggc
      541 tacgcgtgat gcgctgccgc tgatggaaaa tgcataccgt cagatgcaga tgaatgcgca
      601 agctgaaaaa gtagcgaaaa tcatcgccgc aaacagcagc aatacataac agaaacctga
      661 aacacaaaac ggcagccctt gag
//
LOCUS       DAJVPP010000295          681 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVPP010000295 DAJVPP010000000
VERSION     DAJVPP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.306"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>681)
                     /gene="ospC2"
                     /locus_tag="OQM34_004216"
     CDS             complement(<1..>681)
                     /gene="ospC2"
                     /locus_tag="OQM34_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1454397.1"
                     /translation="SEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEA
                     PSGARSSFRSLEHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANL
                     SNNTLNIKSFDKIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHH
                     TVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVH
                     GNQGKTDV"
BASE COUNT          221 a          110 c          125 g          225 t
ORIGIN      
        1 gcacatcagt ttttccctga ttaccatgca catacctgct aacttcatcc acaacctctt
       61 taaagttatc taagaaaaat gattggtgct catggtaaaa aacaggtgga atagcattat
      121 caaagtggtc ggttaatgtc atatatccat atggagagtc atgatcaatg atatacgcat
      181 tagcaccaaa atccacagta tgatgtgttt tatttagtgg gagtgtttct tgatgattgg
      241 aaatttcaac cccaaaaaaa acaaaatcgt ggttagctat ttcttcaata tcctcagaga
      301 atgtgttttt atatgtttgt atattctcag acttaatttt atcaaaggac ttaatattaa
      361 gagtattatt tgacaggtta gcgttacttt gatgtctgaa gttaaaatca gaattaataa
      421 taatagagag caagtgacgt tcatccgaag aaagaggatg agttttttgt attatttcag
      481 aaagatagtg tctggacacc ttatcgaggt gctcaagtga ccgaaatgat gaccgtgctc
      541 cagatggcgc ttcaatagaa agatttaatg atttgaaact ggtcccttga gagaacattt
      601 tactataact ttgcgctgct atagacttcc ttaaaaagct tttcacagta tcagcacagt
      661 gatctaaatg tttttcactt a
//
LOCUS       DAJVPP010000296          670 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVPP010000296 DAJVPP010000000
VERSION     DAJVPP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.307"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            57..554
                     /locus_tag="OQM34_004217"
                     /pseudo
     CDS             57..554
                     /locus_tag="OQM34_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          167 a          167 c          202 g          134 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcgtca atacggtgtt cggtgaacgt ttacttggag catcatgctt
      601 catgaagaaa aactggcacg tcatcaacgc aaacaggcga tgtatacccg aatggcagcc
      661 ttcccggcgg
//
LOCUS       DAJVPP010000297          658 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVPP010000297 DAJVPP010000000
VERSION     DAJVPP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.308"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(584..>658)
                     /locus_tag="OQM34_004218"
     CDS             complement(584..>658)
                     /locus_tag="OQM34_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase"
                     /protein_id="HCS1454398.1"
                     /translation="LDESGNGTFNWQMSPCNLKMAKAI"
BASE COUNT          239 a          108 c          108 g          203 t
ORIGIN      
        1 gacccaaaaa gaacaggtcg tagaaaattg tattcattgg tgtggggccg catagtgatg
       61 tttccacacc cctgatccaa tttaatttta ctaaagagta aatgtagatt taattaatat
      121 attgacagaa gaaaggttat cttaaggaca tccttaaaaa atacggaata agtatttaga
      181 aaaaatagaa agtatatcaa taatgaatat caacagctta aaaaacaaac aatgaacata
      241 taatgcgatt ggcatttatc cacttttgac aatataatat ataaacacta tagccagaat
      301 ttaatcaagt ttcaattaca tcctgcctga ttattcaact ctattttctc tattatctgt
      361 attaataaat gacaatgagt gtcaaacaga tattattcat ttcaacatgt aactttgggc
      421 aaaatcaaga tacgcgcaga agaataattt ccgaatgaat gtagaaagtg ttttatttat
      481 agataaggag agtcttcgat agtagtgagg ttgacagata tccgctgcct aatgagcaat
      541 tatgcccaat gaatctacct cattgggcat aatttggaac acatcagata gcttttgcca
      601 tcttcagatt acacgggctc atctgccagt tgaatgtacc gtttccgctt tcatcaag
//
LOCUS       DAJVPP010000298          648 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVPP010000298 DAJVPP010000000
VERSION     DAJVPP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.309"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..130)
                     /locus_tag="OQM34_004219"
     CDS             complement(<1..130)
                     /locus_tag="OQM34_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HCS1454399.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNEN"
     gene            254..648
                     /locus_tag="OQM34_004220"
                     /pseudo
     CDS             254..648
                     /locus_tag="OQM34_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          222 a          136 c          115 g          175 t
ORIGIN      
        1 tattttcatt ataaccgcga taaggcgatt cattataagc tgcaccagca ccaatggata
       61 attcagaagc agttgcgctt gccataaaag gcaacgcaaa taacgccaca atattgcgat
      121 taattaacat gtccggtatt ccattttaaa ataagataaa aaagagtcgg cataataatg
      181 cttacaattt ctccggtgca agattcatat ttatatagta atatataaaa ttatatataa
      241 ttgggctgtt gcgatgacaa aattacaact taagtaccgg gagttaaaaa ttatctcggt
      301 aatcgctgcc aatgaaaata tcagccatgc cggactgtac ctggcatcgc acaggccaac
      361 gtcagcaaat atcttgctga ttttgaatca aaagtgggtt taaaagtctt tgaccgaacc
      421 acccggcagc tgatgctcac accttttggc accgcgctgt taccgtacat caacgacatg
      481 ctggacagaa acgagcaact taataatttt attgcagatt ataagcatga gaaacgtggt
      541 cgggtcacca tctacgcccc aaccggcata attacctatt tatccaaaca tgtaatcgat
      601 aaaattaaag atatcggtga tatcacctta tcgctaaaaa cctgcaat
//
LOCUS       DAJVPP010000299          643 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVPP010000299 DAJVPP010000000
VERSION     DAJVPP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.310"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            166..>643
                     /locus_tag="OQM34_004221"
     CDS             166..>643
                     /locus_tag="OQM34_004221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCS1454400.1"
                     /translation="MKLKPHYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVSSCH
                     FSRGNYSADESFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLDLHM
                     EQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGIEQFTGIYFRTPASTQSHKIVS"
BASE COUNT          185 a          113 c          155 g          190 t
ORIGIN      
        1 gatctgtgtt aaggctgtct tatgattcag acactatcgg ccgttatctg agaaatatac
       61 gagtaactgg taataacacc tgcaatggta ttgacacaaa cattacagca gaagactctg
      121 tcatcagaca ggtttatggc tgggtatttg ataatgtaat ggtgaatgaa gttgaaaccg
      181 cattatttaa tgcaagggct ctggcactca aaatttatag catgtcaggc tggaacctgt
      241 agagtcggtc ttcacttttt aggccagtgt gtaagtgtta gtgtcagctc ctgccatttc
      301 agcagaggaa attattctgc tgatgaaagc tttggcatca ggattcagcc tcaaacgtat
      361 gcgtggtcgt cagaggcagt taggtcagaa gcaataattt tagacagtga gaccatgtgc
      421 attggtttta aaaatgccgt ctatgttcat gattgccttg atttgcatat ggaacaactg
      481 gatttagatt attgcggctc aacaggcgtg gttatagaga atgtaaacgg aggattttct
      541 ttctcaaact catggatagc agcagatgcc gatggcattg aacaatttac ggggatatat
      601 tttagaacgc cggcctcaac gcagtcacat aaaattgtca gtg
//
LOCUS       DAJVPP010000300          642 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVPP010000300 DAJVPP010000000
VERSION     DAJVPP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.311"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            196..447
                     /locus_tag="OQM34_004222"
     CDS             196..447
                     /locus_tag="OQM34_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454401.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
BASE COUNT          177 a          141 c          156 g          168 t
ORIGIN      
        1 cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcactaaacc cgcttcggcg
       61 ggtttttgtt tttattttca acgcatttga agttctggat ggtgccggaa tagaatcaaa
      121 aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga gtgctactta
      181 tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg tgaagatggc
      241 ctctatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat tgtgtgtttg
      301 caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga cggatttatc
      361 ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatcgg aataaaagct
      421 ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg gctgaactcc
      481 cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa atattacaat
      541 catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc atttaagtca
      601 gactggtacc agcatccctc atgcactgaa gaacaggccg aa
//
LOCUS       DAJVPP010000301          640 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVPP010000301 DAJVPP010000000
VERSION     DAJVPP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.312"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..537
                     /gene="ospC2"
                     /locus_tag="OQM34_004223"
     CDS             <1..537
                     /gene="ospC2"
                     /locus_tag="OQM34_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1454402.1"
                     /translation="NKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLED
                     AIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSKFNFTKQDVAEMEKMNN
                     NMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKANSGDTMLDNAMKSKDSK
                     TIDFLLKNGAVSGKRFGR"
BASE COUNT          235 a           87 c          124 g          194 t
ORIGIN      
        1 aataaaaaca ttgatcctgt tagtctggat agaattataa actttgtgtt tcagcttgag
       61 tatcatatac caagaatgct aagcacagat aacttcaaaa agataagact cagagatata
      121 tctttggagg atgccattaa agcatccaat tatgaagaaa ttaacaataa ggtgactgat
      181 aaaaaaatgg ctcatcaagc tcttgcttat tctctaggca atgcaaaatc tgatatggca
      241 ctttatttac tatctaaatt taattttaca aaacaagatg tcgcagagat ggagaaaatg
      301 aacaacaata tgtattgtga gctgtatgat gttgagtatt tacttagtga agatagtgcc
      361 aactataaag tgctagaata ttttattagt aatggattgg ttgatgtaaa caaaagattt
      421 caaaaagcaa atagtgggga cactatgctt gacaatgcaa tgaaaagcaa agattcaaaa
      481 acaattgatt ttttattaaa aaatggagcg gtatcaggca aacgatttgg gaggtgacct
      541 aaaatatatt taggttgtgc cctgaatctg acgttgtcat ttttgttata ttgatcgata
      601 taggggatgg tgcagaccgt tcccctgcaa caacacattg
//
LOCUS       DAJVPP010000302          623 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVPP010000302 DAJVPP010000000
VERSION     DAJVPP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.313"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..63
                     /locus_tag="OQM34_004224"
     CDS             <1..63
                     /locus_tag="OQM34_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 (AI-2) modifying protein LsrG"
                     /protein_id="HCS1454403.1"
                     /translation="KLESLMTGPRKKRLFNGLMP"
     gene            102..>623
                     /locus_tag="OQM34_004225"
     CDS             102..>623
                     /locus_tag="OQM34_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCS1454404.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMHEVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGC"
BASE COUNT          137 a          162 c          175 g          149 t
ORIGIN      
        1 aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg gttgatgccg
       61 tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aatgtctgac tggaacccct
      121 ctttatatct gcacttttcc gctgaacgat cgcgtccggc ggtggagctg cttgccagag
      181 tgccgctgga gaatgtcgaa tatgttgccg atcttggctg tggcccaggt aacagcaccg
      241 ccctgctgca tcaacgttgg cctgcggcca ggataacagg catcgactcg tctccggcga
      301 tgattgctga agcgcgcagt gctttgccag actgccagtt cgtggaagca gacatccgca
      361 actggcaacc ggaacaggca ctcgatctga tttttgctaa tgcctcactg caatggctgc
      421 ccgaccacta cgaattgttt cctcacttgg tttctttact taatccgcag ggcgtgctgg
      481 cagtacagat gccagataac tggctggagc cgacccatgt gctcatgcac gaagttgctt
      541 gggaacaaaa ctacccggat cgcgggcgtg agccgttggc tggcgttcat gcttactacg
      601 atattttgag cgaagccgga tgt
//
LOCUS       DAJVPP010000303          617 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVPP010000303 DAJVPP010000000
VERSION     DAJVPP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.314"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>617)
                     /locus_tag="OQM34_004226"
     rRNA            complement(<1..>617)
                     /locus_tag="OQM34_004226"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          131 a          188 c          145 g          153 t
ORIGIN      
        1 gatccaaccg caggttcccc tacggttacc ttgttacgac ttcaccccag tcatgaatca
       61 caaagtggta agcgccctcc cgaaggttaa gctacctact tcttttgcaa cccactccca
      121 tggtgtgacg ggcggtgtgt acaaggcccg ggaacgtatt caccgtggca ttctgatcca
      181 cgattactag cgattccgac ttcatggagt cgagttgcag actccaatcc ggactacgac
      241 gcactttatg aggtccgctt gctctcgcga ggtcgcttct ctttgtatgc gccattgtag
      301 cacgtgtgta gccctggtcg taagggccat gatgacttga cgtcatcccc accttcctcc
      361 agtttatcac tggcagtctc ctttgagttc ccggccggac cgctggcaac aaaggataag
      421 ggttgcgctc gttgcgggac ttaacccaac atttcacaac acgagctgac gacagccatg
      481 cagcacctgt ctcacggttc ccgaaggcac attctcatct ctgaaaactt ccgtggatgt
      541 caagaccagg taaggttctt cgcgttgcat cgaattaaac cacatgctcc accgcttgtg
      601 cgggcccccg tcaattc
//
LOCUS       DAJVPP010000304          604 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVPP010000304 DAJVPP010000000
VERSION     DAJVPP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.315"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            200..>604
                     /locus_tag="OQM34_004227"
     CDS             200..>604
                     /locus_tag="OQM34_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1454405.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRW"
BASE COUNT          158 a          161 c          154 g          131 t
ORIGIN      
        1 gcgttacgac aaaacggcga gaaattacct ggcgatggtg aaactgggct gcatccgact
       61 cttttatcaa cgcttacgta attaagggac acagcctaaa tagctgcgcg gaatagtaga
      121 tccctttgag ggaacttagc ccggattgtg cgatctgatc aatcgccaaa tcaaaacaaa
      181 tcaccaaccg gactgagcaa tgccgatcat agcaccaatt tcccgtgacg aacgacgcct
      241 gatgcagaaa gccatccata aaacacacga taaaaattat gcccgcagac tgactgccat
      301 gctgatgctg caccggggcg accgtgtcag cgacgttgcc agaacgctct gctgcgcccg
      361 ttcctctgtt ggacgctgga ttaactggtt cacgcagtcg ggtgttgagg gactgaaatc
      421 attacctgcc gggcgtgccc gtcgctggcc gtttgagcat atctgcacac tgttacgtga
      481 gctggtaaaa cattctcccg gcgactttgg ctaccagcgt tcacgctgga gtacagaact
      541 gctggcaata aaaatcaatg agataaccgg ttgccagtta aatgccggaa ccgttcgccg
      601 ctgg
//
LOCUS       DAJVPP010000305          602 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVPP010000305 DAJVPP010000000
VERSION     DAJVPP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.316"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>602)
                     /gene="ipaH4.5"
                     /locus_tag="OQM34_004228"
     CDS             complement(<1..>602)
                     /gene="ipaH4.5"
                     /locus_tag="OQM34_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCS1454406.1"
                     /translation="PLPLHIRELNISNNELISLPENSPLLTELHVNGNNLNILPTLPS
                     QLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPELPSTLTILRIEGNRLTVLPE
                     LPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRLSRLPQELLALDVSNNLLTSL
                     PENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYHGP"
BASE COUNT          178 a           87 c          153 g          184 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggtta ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gt
//
LOCUS       DAJVPP010000306          592 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVPP010000306 DAJVPP010000000
VERSION     DAJVPP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.317"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..435
                     /locus_tag="OQM34_004229"
                     /pseudo
     CDS             1..435
                     /locus_tag="OQM34_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT          161 a          110 c          144 g          177 t
ORIGIN      
        1 aggttattgg gggaacgcag gctgcgttga atgaatttac cacgctgctg atggccgctg
       61 gatacgaacc ggttaaactc ggtgtgagcg gtgcctggca cacgcgtttg atggaagacg
      121 gcgttcaggc aatgcgcgat tatcttgccg gactggatat cgcttcgcca gagcatcagg
      181 ttctgatgaa cgtaacagca aaaagcgagg tcgctccgtc aattatcaaa gaaaatctct
      241 cattacactt aactcatacg gtgaaatgga ctgaatctct cgatacatat tctgaatatg
      301 ccaaccccgg tagctttcct tgaaataagt aataagccct atttggggaa tatgttaaat
      361 gattttgctg gcgtggatca gcaacgagtt atgcattgtc gtaaagcatt tagtgatgca
      421 aaggtattca aatgattcct gattatttaa cttttattcg ctttcaggat aaacgaaatc
      481 tgatatacat ttatgctatt ggacttattc tgataggcat tttattggaa aaatgcaggg
      541 tttacttttc catcagagga tattggtgta gttagtggga ttctggccct gg
//
LOCUS       DAJVPP010000307          590 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVPP010000307 DAJVPP010000000
VERSION     DAJVPP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.318"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>590
                     /locus_tag="OQM34_004230"
     CDS             <1..>590
                     /locus_tag="OQM34_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCS1454407.1"
                     /translation="GVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQDWFLKYE
                     LKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAGGSSIELA
                     EAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIAELNGEGE
                     VAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGV"
BASE COUNT          143 a          136 c          193 g          118 t
ORIGIN      
        1 gggagtcagc gccgagctgg gaccagatgc cacgggtgtt ggctggatct atgaatatgc
       61 actggtggat cgcagcggta agcacgatct tgccgattta cgctcattgc aggactggtt
      121 tctcaaatat gagctgaaaa ccattcctga cgttgcggaa gtggcgtcgg taggcggtgt
      181 ggtgaaagag tatcaggtgg ttatcgatcc tcagcgcctg gcgcagtatg gcatcagtct
      241 cgccgaagta aaaagcgcgc tggatgcttc aaaccaggaa gcgggcggtt cgtcaatcga
      301 actggcggaa gcagaatata tggtgcgcgc cagcggctat ctacaaacgc tcgacgactt
      361 taatcacatc gttttaaaag ccagtgaaaa tggcgtgccc gtttatctgc gcgatgttgc
      421 gaaggtccag attggcccga agatgcgccg gggcattgcc gaactaaacg gcgaaggcga
      481 agtggcgggc ggggtggtga tcctgcgatc cggcaaaaac gcccgagaag tgatcgccgc
      541 cgtgaaggac aaactggaaa cgctgaaaag tagtctgccg gaaggcgtgg
//
LOCUS       DAJVPP010000308          588 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVPP010000308 DAJVPP010000000
VERSION     DAJVPP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.319"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(219..>588)
                     /locus_tag="OQM34_004231"
     CDS             complement(219..>588)
                     /locus_tag="OQM34_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454408.1"
                     /translation="YQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVNAGD
                     SLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPP
                     CSGQAFPADNNGYAPPPCRK"
BASE COUNT          153 a          138 c          151 g          146 t
ORIGIN      
        1 ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat tggaaaccag tgagttatcg
       61 tattgatgac ctaaggtgct catggatgga ctcctattac aaagtcgata taaaaaaacc
      121 cttccgcgtc gttaggcccg acgaggaagg gttggatttg cgaaacacaa gtttgtgcta
      181 attatcgcct taaatgggcg aggcatcaag cgaggttgct atttccgaca cggtggcggc
      241 gcatagccat tgttatctgc cggaaaggct tgtccagagc acggcggcag cgcgttttgc
      301 gccgggcctt gtaattcaaa ggcggcaaag ccgattaccc cggtattacg cacatcgcct
      361 tgcgccgaat tggctgcgta ggaatcagca acgttggcga actgaaaagc cgcttcagta
      421 tgtttatctt tgcggaatcc tttaatcact aacgaatctc cggcattcac aatatagccg
      481 ttgttattca gcgaaccttg cttgccgttc agcacatcca ggccatcagc cgtagcaacg
      541 atctcgtagt tggtgttacg actgtaatta cggacatata attgataa
//
LOCUS       DAJVPP010000309          581 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVPP010000309 DAJVPP010000000
VERSION     DAJVPP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.320"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..454)
                     /locus_tag="OQM34_004232"
     CDS             complement(<1..454)
                     /locus_tag="OQM34_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCS1454409.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWD"
BASE COUNT          166 a          146 c          118 g          151 t
ORIGIN      
        1 catcccataa cgaagatgtt tggctatttt tactttgcgg caaaatcaca cttagctcat
       61 taaataaggt ttttatgact tcttttccct gccccgcggc aattaccgga caagaatgcg
      121 gttgcagtaa ctgattttcc tcagccccct gaaactgaaa gccgtacagc gcacaggtac
      181 acgtcgttgc cggatcgtta acgtcagagg ccaccgcatg cagcctgccg cgttctatca
      241 ccacaatgtc atccgcatgt gcgacatata acgaagaatc gatggtaaac cttgcaaccc
      301 ctttcttgac gtaaatcagc tcggtttcat tgtcatgaac gtgatgtcca gactcccatt
      361 ttggatcatc gctaaatgca aaacgtgaaa gccgtggcgt tttaccggcg acaaacagcg
      421 tttcactggc attatcaaaa cagcgttgat acatgacaac ctccctattc catgagcaag
      481 caaaaacaat atatgccgga taaaatacgg cgtgtattgc ggttatacaa ccgcgtttag
      541 cgagagctaa acaagattat ttacactgaa tgcaagattg t
//
LOCUS       DAJVPP010000310          566 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVPP010000310 DAJVPP010000000
VERSION     DAJVPP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.321"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..566
                     /locus_tag="OQM34_004233"
                     /pseudo
     CDS             1..566
                     /locus_tag="OQM34_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          140 a          160 c          160 g          106 t
ORIGIN      
        1 atcacagctc tacaactggc gcagtaaaca gcaaaatcag cagacgtctt ctgaacgtga
       61 actggagatg tctaccgaga ttgcacgtct caaacgccag ctggcagaac gggatgaaga
      121 gctggctatc ctccaaaagg ccgcgacata cttcgcgaag cgcctgaaat gaagtatgtc
      181 tttattgaaa aacatcaggc tgagttcagc atcaaagcaa tgtgccgcgt gctccgggtg
      241 gcccgcagcg gctggtatac gtggtgtcag cggcggacaa ggataagcac gcgtcagcag
      301 ttccgccaac actgcgacag cgttgtcctc gcggctttta cccggtcaaa acagcgttac
      361 ggtgccccac gcctgacgga tgaactgcgt gctcagggtt acccctttaa cgtaaaaacc
      421 gtggcggcaa gcctgcgccg tcagggactg agggcaaagg cctcccggaa gttcagcccg
      481 gtcagctacc gcgcacacgg cctgcctgtg tcagaaaatc tgttggagca ggatttttac
      541 gccagtggcc cgaaccagaa gtggcc
//
LOCUS       DAJVPP010000311          562 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVPP010000311 DAJVPP010000000
VERSION     DAJVPP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.322"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(184..>562)
                     /locus_tag="OQM34_004234"
     CDS             complement(184..>562)
                     /locus_tag="OQM34_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HCS1454410.1"
                     /translation="VKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGVLYYDE
                     NFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYSELPEE
                     ITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT          137 a          163 c          148 g          114 t
ORIGIN      
        1 ggcgaaggcg aaatgcggat ctgggtgaat gaggaaaagc atcgccatcc ggaggttgga
       61 taaggcgctc gcgccgcatc caacgtaggg agcacattgc ctgatgcgac gcttaacacg
      121 tcttatcagg catacgagtc ggtgcgagag cgtaggccag ccgcaccgac atccaccggt
      181 gaattaaaaa cgccagctca cgccggtgac aaaggtaaaa ctctcattac gatcaatcat
      241 cgggctgtcg gtaatctctt ccggcagctc gctgtatccg gcgctcgcca tcaatacgac
      301 gtgctctcct atcgggtatt ttgccgtcag gctgacatag ggcacccatg catcctgcgc
      361 ggaataactt gccagaccgc tacgacggga ctcactctct gaaatgccat agtaatagtc
      421 actgaaattc tcgtcataat agagaacgcc cagcgcaggt gtcagcgaca ggcgacctat
      481 ctgcattttg tggaataccg atagctcccc cacccagccg ttgctgttat ccagaacgtc
      541 cgcagccgca gaggctttca cg
//
LOCUS       DAJVPP010000312          557 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVPP010000312 DAJVPP010000000
VERSION     DAJVPP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.323"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>557)
                     /locus_tag="OQM34_004235"
     CDS             complement(<1..>557)
                     /locus_tag="OQM34_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCS1454411.1"
                     /translation="VSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALL
                     PLISGVVLVALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILK
                     TILVGSVTGFFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVF
                     FQIFSHLKKLRMSRQDIRDEFKQSE"
BASE COUNT          150 a          160 c          132 g          115 t
ORIGIN      
        1 cttcgctttg tttgaactca tcacgaatat cctgccgcga cattcgcagc tttttcaggt
       61 ggctgaagat ttggaaaaag acgtcaaatc ccaccattgg aatgacacca agcaccacca
      121 gcagtgcgca tagccctacc aaatccatcg cattacccat ggcggtaatc ggtgactcgg
      181 ccattaagcg catcatctgc ggccagtgat gccagagaaa aaaccccgtc acgctgccaa
      241 ccaggatggt tttcaaaatt gccttaagca actccgcgct agtctgtgcc gagaatatcc
      301 gtttaatgcc cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa
      361 ataccagccc ccccagcata accggagaaa tgagcgccac cagcaccacg ccgctaatca
      421 gcggtagcag cgccagcatg gcttctctga tcagcagaat aatttgcccg aggatcagat
      481 tcgggtcttt gataatactg tgatcaaaat gcagcccagc ggagagcatg cccgacaatc
      541 gacgggccag cgacaca
//
LOCUS       DAJVPP010000313          557 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVPP010000313 DAJVPP010000000
VERSION     DAJVPP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.324"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..113)
                     /locus_tag="OQM34_004236"
     CDS             complement(<1..113)
                     /locus_tag="OQM34_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454412.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWD"
BASE COUNT          151 a          116 c          106 g          184 t
ORIGIN      
        1 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
       61 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      121 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      181 acaatcctag aactttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      241 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
      301 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
      361 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
      421 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
      481 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
      541 ttatgaatgg tgcagtc
//
LOCUS       DAJVPP010000314          551 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVPP010000314 DAJVPP010000000
VERSION     DAJVPP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.325"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            31..551
                     /locus_tag="OQM34_004237"
                     /pseudo
     CDS             31..551
                     /locus_tag="OQM34_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          116 a          143 c          156 g          136 t
ORIGIN      
        1 agtgtatgat ggtgatttta aggtgcttgc gtggcttcca tttccatcag atgtccttcc
       61 tgctccgcta ctgaaggcgt ggtgcgtaac ggcaaaagca ctgccggaca tcagcgctat
      121 ctctgctctc attgccgtaa aacatggcaa ctacagttca cttacaccgc ctctcagccc
      181 ggtacgcacc agaaaatcat tgatatggcc atgaatggcg tcggatgtcg cgccagtgca
      241 cgcattatgg gcgttggcct caacacggtt ttacgtcact taaaaaactc aggccgcagt
      301 cggtaacctc gcgcatacaa ccgggcagtg atgtgattgt ctgcgctgaa atggacgaac
      361 attggggcta cgtcggtgct aaatcacgtc agcgctggct gttttacgcg tatgacagga
      421 tacggaggac ggttgtggcg cacgtcttcg gtgaacgcac tctggccaca ctggagcgtc
      481 ttctgagcct gctgtcggcc tttgaggtcg tggtatggat gacggatggc tggccgctgt
      541 atgaatcacg c
//
LOCUS       DAJVPP010000315          543 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVPP010000315 DAJVPP010000000
VERSION     DAJVPP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.326"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..288)
                     /locus_tag="OQM34_004238"
     CDS             complement(<1..288)
                     /locus_tag="OQM34_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS1454413.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWK"
     gene            complement(292..>543)
                     /locus_tag="OQM34_004239"
     CDS             complement(292..>543)
                     /locus_tag="OQM34_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454414.1"
                     /translation="LGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVR
                     WDSPPTLDGMTGHAGELPNCRCYKEIVFVRVPFAMKRAA"
BASE COUNT          117 a          153 c          136 g          137 t
ORIGIN      
        1 tttccagttc tcgggattta ccagccgcac attcccgttt tcatcttcag gatgcaggat
       61 cgtgatgctc atcccttcga atgaggcaag cgtggccgga tggaatacct gctcaggaga
      121 acgcgtgacg actatttcaa cgaacttatc gggtttcagt tttggcaggt catcagcacc
      181 atagagctgc ttacctgttc gtcctatcgg cacgtctttg cacagcaacg agccgtcagc
      241 caggcggtat cgggtttccc cgagcctggt ctcaaaaaag tatttcatgg gttatgctgc
      301 ccttttcatt gcgaatggaa cgcgaacaaa cacgatctct ttatagcagc ggcagtttgg
      361 cagctcgccc gcgtggccgg tcatgccgtc caaagttgga ggtgaatccc agcggacgaa
      421 tttacctttc atgtgatcgt gggaatctcg gacatcgcca tcatcggctg tacgccaaat
      481 ataaccatcc gagccaatag ctatcgctcg ggccatatcc agcgcgcccg ttgctcttcc
      541 cag
//
LOCUS       DAJVPP010000316          540 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVPP010000316 DAJVPP010000000
VERSION     DAJVPP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.327"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>540
                     /locus_tag="OQM34_004240"
     CDS             <1..>540
                     /locus_tag="OQM34_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HCS1454415.1"
                     /translation="YLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTL
                     TMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGAAS
                     WAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWSRE
                     QVKESREEPIWLAPRQMPQL"
BASE COUNT          112 a          139 c          163 g          126 t
ORIGIN      
        1 ttaccttgaa gcgctgatca attatgaaga tcgctggttc tggaagcatc cgggggttaa
       61 tccattctcg gtggcgcgcg cagcatggca agatctcact tcgggacggg ttatttccgg
      121 tggcagcacg ctcactatgc aggttgctcg tctgcttgat cctcacccca aaacatttgg
      181 tggcaaaatt ctccagctct ggcgcgcgtt gcaactggag tggcatttgt ctaagcgtga
      241 aattctgatc ttgtatctta accgcgctcc gtttggcggt acgttgcagg ggatcggtgc
      301 ggcaagttgg gcttatctcg gaaaatcgcc tgcgaattta agctattccg aggcggcaat
      361 gctggcggtg ttaccgcaag tgcccagccg actgcgcccg gatcgttggc cggagcgtgc
      421 cgaagccgcg cgtaataaag tgctcgaacg gatggctgcg caaggtgtgt ggtcccgtga
      481 gcaggtaaaa gagtcaaggg aagaacccat ctggctggca ccccgacaaa tgccgcaact
//
LOCUS       DAJVPP010000317          538 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVPP010000317 DAJVPP010000000
VERSION     DAJVPP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.328"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..359
                     /locus_tag="OQM34_004241"
     CDS             <1..359
                     /locus_tag="OQM34_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454416.1"
                     /translation="VVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYK
                     YRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYR
                     PLQKLINGTWYNVASI"
     gene            362..>538
                     /locus_tag="OQM34_004242"
     CDS             362..>538
                     /locus_tag="OQM34_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1454417.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRV"
BASE COUNT          164 a          108 c          136 g          130 t
ORIGIN      
        1 ctgttgttgc agagcttggt gatgattcag gctggcattt tttcagccag agaaacccgg
       61 ataacagcat agtgttcact gttaacggac aggtaattcc gttaaattac ggaaacttcg
      121 atgcccgcta taaatatcga acagaggggg tacaggatgt acggtatggc catgaaatgt
      181 attacagccc cggcagtaac accgtttcgt ggagattttg cgcaccttcg ggacacgggc
      241 tgtcagggat ggcgatatcg gataccggcc gtaactcagc ggataacgtt gacggtgtgt
      301 attaccgacc actgcaaaaa ctgattaatg gcacctggta taacgtagcg agtatttaac
      361 aatgttgcat ttaaaaaata ttactgcggg taatccgaaa acagcagaac aatatcagat
      421 gacaaaacga tattcggtca cctggctttt ttcagaagac ggaaaaaact ggtatgaaga
      481 gctgaagaat ttcgccagcg acacaataaa aatagcttac accggagatg gtcgcgtg
//
LOCUS       DAJVPP010000318          535 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVPP010000318 DAJVPP010000000
VERSION     DAJVPP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.329"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            119..>535
                     /locus_tag="OQM34_004243"
     CDS             119..>535
                     /locus_tag="OQM34_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1454418.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADV"
BASE COUNT          106 a          149 c          160 g          120 t
ORIGIN      
        1 tcgcccgctg cacggagcgg gaatttgcga ccattcttgc cggtatcagc gccggcggta
       61 tgtactgccc ggagtgtggc acggttcact ggccggacgg agtcgccccg cccttctgat
      121 gctcccccgt tttgccgata tttttcagca gggaaaccgc tggcttaact ggctggagaa
      181 acaaccggaa ggggcagtcc gtccggtggt aattgaatca gtgacaaaaa tcatggcctg
      241 cgggaccaca ctgatgggct acacgcagtg gtgttgttca tcaccggact gttgccatac
      301 aaaaaaggtc tgcttccggt gtaaaagccg ctcctgcccg cactgcgggg tgaaggctgg
      361 cgcacagtgg atacagtatc tgctgagcct ggtcccctac tgcccgtggc agcatattgt
      421 gttcacactt ccctgccagt actggtccct ggtgttccac aaccggtggt tactggcaga
      481 gatgagccgc attgcagcgg atgtgatact ggaaatctgc catcaggcag atgtg
//
LOCUS       DAJVPP010000319          534 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVPP010000319 DAJVPP010000000
VERSION     DAJVPP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.330"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..460
                     /locus_tag="OQM34_004244"
     CDS             <1..460
                     /locus_tag="OQM34_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454419.1"
                     /translation="RVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQVLPLF
                     LLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIVHIKGM
                     ASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT          120 a          109 c          126 g          179 t
ORIGIN      
        1 tcgggtaata catcacggac tttattttgc ggttttagga ccgttaattg gtgttctgtt
       61 tcttgtcctc tacatattct tcgcaaaaga accgctggtt cttttggtaa taatacaggt
      121 acttccattg tttctcttat tgtcgattac tacgggagct attcctgcat tgttaaccgg
      181 cgtgatggtc gcctgtctgc cggaaaagat cgggtcacag aaaaattatc gttgtctggc
      241 tggtggcata ggtggcgtcg ttatcaccga gatctattgt gcagttattg tacatattaa
      301 gggcatggct tcctcggagt tgtttgaaaa cattctttct ggtgacagtc tcgttgtccg
      361 catcattcct gcattgctgg caggtgtggt gatgagcaga atcattaccc gtctgcccgg
      421 attggatatt tcatgtcctg aaacagactc tttaagttaa gcgggatact ttatctttgg
      481 gctactcaaa agcagacagg atgttactat gactcaaaat atcaggccgt tacc
//
LOCUS       DAJVPP010000320          529 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVPP010000320 DAJVPP010000000
VERSION     DAJVPP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.331"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(288..440)
                     /gene="hokE"
                     /locus_tag="OQM34_004245"
     CDS             complement(288..440)
                     /gene="hokE"
                     /locus_tag="OQM34_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1454420.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
BASE COUNT          142 a          150 c          110 g          127 t
ORIGIN      
        1 ccccaggatt gatagaaata tctcttgggg cttcatcttt ccaaacctca gagttaacct
       61 gaaaccggaa agcttcaggc acccgccgtt atcttaccct tccttagcaa aatagctgtt
      121 tatttataca gtgagtcctg tttcggactt ctgtcggcaa aactcatcat tgctcttaaa
      181 acgaaatcgc ccgcagccat cactgaccgc gggcgaaagt gtaaagcagg tgccttacca
      241 tcctgacctg acaaccggat atgcggggat tactccccgc acaacggcta cttcttcggt
      301 tcgtaagcga gaacagccct gaactcaata ttacgttcct tcaccgtaaa ctcacacagc
      361 gagtctccgg ccagaagcgt aaatcccagt accgtcaaac acagcactat gactgccaca
      421 agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac taccatccaa
      481 attgtctggt ttggagtggc agccccaggg tgtactgacc ccaaaaagt
//
LOCUS       DAJVPP010000321          524 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVPP010000321 DAJVPP010000000
VERSION     DAJVPP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.332"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>524)
                     /locus_tag="OQM34_004246"
     CDS             complement(<1..>524)
                     /locus_tag="OQM34_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISEc37 family transposase"
                     /protein_id="HCS1454421.1"
                     /translation="VMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKREWNRFLDTHY
                     RRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREE
                     YLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAMQIT
                     WRGMYQRLLKVDPL"
BASE COUNT          114 a          166 c          107 g          137 t
ORIGIN      
        1 ttcagcgggt caaccttcag taacctctga tacatccctc tccaggtgat ttgcatcgcc
       61 gtttttctca ctgtctccgt tatgatgtac accacttctt ccagtaaccg ccgtttcgcc
      121 ggactcaaaa aaccgtagta cctcaccata cggaacccct tatccgccac atgccaggag
      181 aacctttcca tgaactcatc tccactcatc aacaggtatt cttcccgttt tgttcggtga
      241 ctgttgtaac gcagaccgat ttcatcctga ccggcataat gctccagacg actcatcggc
      301 accggtggct ttttcaggta agagccaaag tacaccgcca catgggtggc attatccatt
      361 acccgggata cgttgacatt ccagccacgg cggtaatgcg tgtccaggaa gcgattccat
      421 tcccgtttac tgcttccttc tgctgccagc gcatccggca tcaccaggtc agggtatttc
      481 cgtgacagca accgtgttat ccggtagcgc cacatgctca tcac
//
LOCUS       DAJVPP010000322          513 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVPP010000322 DAJVPP010000000
VERSION     DAJVPP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.333"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>513
                     /locus_tag="OQM34_004247"
     CDS             <1..>513
                     /locus_tag="OQM34_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCS1454422.1"
                     /translation="IILKQGIVWCTSLPGNRVLLSRIPVVPDSNLLLAPAIDTVNRLP
                     ILLYQSQFADTRILVTISDQHIRGALNVPLKGVRYVLRVADDIIGPTGDVMTLNGHYP
                     YTEKVHSTKYHFTIIFNPPPLFSFYRLIDKGFGILIFILLIACAAAFLLDRYFNKSAT
                     PEEILRRAIN"
BASE COUNT          131 a          119 c          110 g          153 t
ORIGIN      
        1 tcatcattct caaacaggga atagtctggt gtacatccct gcctgggaat cgggtcctgc
       61 tgtctcgtat tcctgttgtc ccggacagta atttactgtt ggctccggca atcgacaccg
      121 ttaatagatt acctatcctg ctctatcaga gccaatttgc agatacgcgc attttggtta
      181 cgataagtga tcaacatatt cgcggggcgc ttaatgtacc cttgaaaggg gtaaggtatg
      241 tattacgcgt ggcggatgac attattggac caacgggtga tgtgatgacg cttaatggac
      301 attatcccta taccgagaag gttcactcca caaaatatca tttcactatt atctttaacc
      361 cgccgccact ctttagcttc tacagactta tcgataaagg ctttgggatt ttaatattta
      421 ttctgttaat cgcctgcgcc gctgccttcc tgcttgatag atatttcaat aaaagcgcaa
      481 cgcctgaaga gatcctgcga cgggcaataa ata
//
LOCUS       DAJVPP010000323          505 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVPP010000323 DAJVPP010000000
VERSION     DAJVPP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.334"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(220..>505)
                     /locus_tag="OQM34_004248"
     CDS             complement(220..>505)
                     /locus_tag="OQM34_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1454423.1"
                     /translation="APALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTV
                     GSLLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          175 a          107 c          115 g          108 t
ORIGIN      
        1 tcccagccat agacaaatcc ttcgccaaaa ttaaaggggc atctgtaaaa cgagggatta
       61 tgcgcggcaa ataagaagtg cggaaatagc gaaatgcaaa attataacct gctgcacatt
      121 ttatttttaa acgaataaaa tttggaaaaa agcaagtcag atagaagaaa caaaaaagca
      181 gagtgtgaaa cagtaagttc acactttgct tgaattcatt tacacgtgag caacgggttt
      241 cggcgtatga ccggcgttgc gaagcagcat cagcaaatag ataaacgata cgctggcgat
      301 catgataaac aataaattat cggagaaatt ctgcattagc atagcgctaa atgacgggcc
      361 aagcagactt cccacggtat agctcaacag taaggcttgg ttcatcgcca ccagttgatg
      421 atgttcaact ttctcacaag cccaggccat cgccaccgga tatagcgtaa agccagcggc
      481 accgaggatg aataacgctg gggcc
//
LOCUS       DAJVPP010000324          503 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVPP010000324 DAJVPP010000000
VERSION     DAJVPP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.335"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..275)
                     /locus_tag="OQM34_004249"
     CDS             complement(<1..275)
                     /locus_tag="OQM34_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1454424.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQG"
BASE COUNT          121 a          106 c          111 g          165 t
ORIGIN      
        1 atgccctgtt gcatcccttt gccaatttca tgtttgttgg cttttgcttc aatcacggct
       61 aagggaatgc ctggcttgtg gtacagcacg atgtcagcag attttaccgt tctgcgtgcc
      121 gcaactttac cacgtacgat gactttaccg tcccggagtt tgacctcctg tctgatttgc
      181 gttgtgttgt cccagcctgc attgagaatg gctggcataa cgcatttggt aatgatgtct
      241 gcttccgtca ggttacttag gttcagttcc gccatggggg gttctcgctt ttatcaggac
      301 gattctgatt gtacataaaa tcaatagcct ggctatgatt tagggcggtg tttcaacgta
      361 aagtacgtag cagagatcac attgatatct ctgttatatc ttgcttgaat taatctgggc
      421 atatcagaga cagataaaca gaagttacag gaaccatatc aactctaaca aaatatataa
      481 aaatcttata gttgcgtttc ttt
//
LOCUS       DAJVPP010000325          499 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVPP010000325 DAJVPP010000000
VERSION     DAJVPP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.336"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            43..>499
                     /locus_tag="OQM34_004250"
     CDS             43..>499
                     /locus_tag="OQM34_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454425.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRE"
BASE COUNT          163 a           91 c          113 g          132 t
ORIGIN      
        1 aaaattaatt atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag
       61 ggtgttaata ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt
      121 aaagaacctc gcaataaaga atcgttgttc tttatgtctg tcatggaact gcgcgacctg
      181 ctgattgcgc tggaaagtcg tctgcatcaa aagcataaac tggatgctgc tgcacgcctt
      241 cagtatgagc aagctcgcga taaagtcatt aagaaaatgg cagaaaacat cccagaaatt
      301 ctggttgatg agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc
      361 gataatcaag gcgaaaatct taccttcgta ttaacgctac atgacggtag caaatgtgag
      421 ttagtcgtca atgagttgca gattgaaatg ctggcgcgag caatcattca tgcaattaat
      481 aatgcggaaa tgcgtgaac
//
LOCUS       DAJVPP010000326          499 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVPP010000326 DAJVPP010000000
VERSION     DAJVPP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.337"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>499
                     /locus_tag="OQM34_004251"
     CDS             <1..>499
                     /locus_tag="OQM34_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1454426.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVG
                     RKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPW
                     NFSSVK"
BASE COUNT          145 a          105 c          152 g           97 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctga acgagttctg
      241 tcgtgacggc tgggtggaaa tagacaacaa catcggtgaa aacgcgttac gatcggtggc
      301 ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat
      361 catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg actggttgcg
      421 cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg
      481 gaacttctcg tctgtaaaa
//
LOCUS       DAJVPP010000327          495 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVPP010000327 DAJVPP010000000
VERSION     DAJVPP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.338"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>495)
                     /locus_tag="OQM34_004252"
     rRNA            complement(<1..>495)
                     /locus_tag="OQM34_004252"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           92 a          165 c          109 g          129 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcag
//
LOCUS       DAJVPP010000328          484 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVPP010000328 DAJVPP010000000
VERSION     DAJVPP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.339"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>484
                     /locus_tag="OQM34_004253"
     CDS             <1..>484
                     /locus_tag="OQM34_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1454427.1"
                     /translation="STLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYMSDN
                     ASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKYK
                     DLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVI
                     "
BASE COUNT          132 a           93 c          134 g          125 t
ORIGIN      
        1 agagcactct ggcattagtg gtgatgggca ttgtggcatc tgcatctgta caggctgcag
       61 aaatatataa taaagacggt aataaactgg atgtctatgg caaagttaaa gccatgcatt
      121 atatgagtga taacgccagt aaagatggcg accagagtta tatccgtttt ggttttaaag
      181 gtgaaactca aattaacgat caactgactg gttatggtcg gtgggaagcg gagtttgccg
      241 gaaataaagc ggagagtgat actgcacagc aaaaaacgcg tctcgctttt gccgggttga
      301 aatataaaga tttgggttct ttcgattatg gtcgtaacct gggcgcgttg tatgacgtgg
      361 aagcctggac cgatatgttc ccggaatttg gtggcgattc ctcggcgcag accgacaact
      421 ttatgaccaa acgcgccagc ggtctggcga cgtatcggaa caccgacttc ttcggcgtta
      481 tcga
//
LOCUS       DAJVPP010000329          484 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVPP010000329 DAJVPP010000000
VERSION     DAJVPP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.340"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            200..>484
                     /locus_tag="OQM34_004254"
     CDS             200..>484
                     /locus_tag="OQM34_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1454428.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPA"
BASE COUNT          157 a           86 c           74 g          167 t
ORIGIN      
        1 tattaaatta atattgtgaa tatttataaa ttcctaaaaa ataagtatcg tcattgtgaa
       61 agctactaaa tttcagtaag ttaattccca gcctttaaat ttaatgttaa ctaaaaatgc
      121 ttttttatat tgacttacaa taaatctaca tctctaatac accaactttt ttgaaacgat
      181 attgtaagat caaaattata tggtggcgaa aattaatctc ttattacggc ttacgccgct
      241 tttattcgtg ttcgctcctt ttttggcaca gtcaaatatg actgtttatc ctatgcctgt
      301 ttccattaat agtcagggtg aaggcaacgt tcgcgtaata tctaaaagta atgaagttca
      361 gtacatcaag gcgacagtat tccgtatcga taatccatcg acgcctcagg aaaatgaagt
      421 tgagattaaa tccggtgatg caaatcatct ggttgttatg ccacctaaat tcgctttacc
      481 agcc
//
LOCUS       DAJVPP010000330          475 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVPP010000330 DAJVPP010000000
VERSION     DAJVPP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.341"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>475)
                     /locus_tag="OQM34_004255"
     CDS             complement(<1..>475)
                     /locus_tag="OQM34_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1454429.1"
                     /translation="GPGNMLMDAWIWRQAGKPYDKDAEWARAGKVILPLLQNMLSDPY
                     FSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAVTISEQVLLSGGCER
                     LMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAFAWLAWRTLAGLP"
BASE COUNT          113 a          139 c          122 g          101 t
ORIGIN      
        1 tggtaatccc gccagcgtcc gccaggcaag ccaggcgaaa gccaatgctt ccatgtcatc
       61 gccactaatg ccaacggcat cggtggtggt gacttctgtg cctagcagta ataccgccag
      121 acgcgccatg agtagcggat tacgactacc tccaccacat accatcaatc gttcgcagcc
      181 accgctcaac aaaacttgtt cagaaatggt cacggcggtg agttctgcca gtgtggcctg
      241 aacatcacgg ggatcaacac ccggaaaatg gcgtaaatgg cgctccagcc agccgtagtt
      301 aaagtattcg cgtccggtgc ttttcggtgc aggttgcgag aaatacgggt cgctgagcat
      361 attttgcagc agtgggagaa taactttacc cgcccgtgcc cactcggcat ctttatcgta
      421 aggtttacct gcctgacgcc agatccaggc atccatcagc atgttaccag gaccg
//
LOCUS       DAJVPP010000331          474 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVPP010000331 DAJVPP010000000
VERSION     DAJVPP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.342"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(6..>474)
                     /locus_tag="OQM34_004256"
     CDS             complement(6..>474)
                     /locus_tag="OQM34_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCS1454430.1"
                     /translation="VIAAFSVAVMLALVVCVVWQVFSRYVLNQPSTLTDELARFLMIW
                     VGLLGAAYTVGTQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVIVTGGLKLID
                     KTLATSQVSAAMQISMGYVYIILPLSGVVMMFYALCFINQSIQQLKQPAQEAS"
BASE COUNT          153 a          105 c           98 g          118 t
ORIGIN      
        1 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
       61 cacaaggcat aaaacatcat taccactcca cttaatggca gaatgatata tacgtatccc
      121 atcgatattt gcatagcggc agatacctgt gatgttgcca gtgttttatc aattaatttc
      181 aggccaccag tcacaatgac cgaaccggca aaacctaaaa tgagaacgtt aatgactata
      241 ctaagaagta attgtttgcg cttattcagg gcgagagcaa ataaatcaat agagagatgt
      301 cgctgcgtgc cgacggtata tgcagcacct aataatccta cccagatcat caaaaagcgt
      361 gccagctcat cggttaatgt acttggctgg ttaagtacgt agcggctaaa tacttgccag
      421 actacgcata caaccagagc aagcattacg gcgacagaaa acgcggcaat gacg
//
LOCUS       DAJVPP010000332          471 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVPP010000332 DAJVPP010000000
VERSION     DAJVPP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.343"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            17..>471
                     /locus_tag="OQM34_004257"
     CDS             17..>471
                     /locus_tag="OQM34_004257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCS1454431.1"
                     /translation="MPKMKRRPASANLMTNRRSRQAEIRYRTSLRRIARAVGDIVNGR
                     YDGSNDSITEIIAALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRN
                     LVESAPVGQVMQSIIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRA"
BASE COUNT          130 a          124 c          140 g           77 t
ORIGIN      
        1 aacgaggaca tcgagaatgc cgaagatgaa gcgccgcccg gcatcggcga acctgatgac
       61 gaaccgcagg agccgtcagg cggaaatccg gtatcgaacc agcctacgca ggatagcgag
      121 ggcggtcgga gacatcgtaa atggtcgcta cgatggttca aatgacagta tcacggaaat
      181 tattgcggcg ctggagcgtt acagtgaaat catcaccccc tgggcgacaa aggtcgcgga
      241 aaactttact gcggacctaa cccggcagaa cgagaaagtt tggcggcaac acagcaagaa
      301 catcagtcgc gagctccgca atcttgtgga aagcgctcct gtgggccagg tgatgcaatc
      361 catcatcgcc gaacaggtca agtacatcaa atctctgcct ctcgaggccg cagacagggt
      421 gtacgacatc cagaacaaag cgatagaggc tgttgtcacc ggtggtcggg c
//
LOCUS       DAJVPP010000333          466 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVPP010000333 DAJVPP010000000
VERSION     DAJVPP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.344"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>466
                     /locus_tag="OQM34_004258"
     CDS             <1..>466
                     /locus_tag="OQM34_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1454432.1"
                     /translation="EFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTDAKGEKRPMY
                     QITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPGDGRTLVV
                     HFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLLS"
BASE COUNT          130 a          120 c          107 g          109 t
ORIGIN      
        1 cggagttctt ccggaaaatg cacaaaaacg ttattcagaa gatcgaaact ctcgaatgct
       61 cgccggagtt taaccggctt aattttaagc ccgtcaccta taccgacgcc aaaggcgaaa
      121 aacgcccaat gtaccaaatc accaaaaacg gcttcgtttt cctggtgatg ggcttcactg
      181 gcaaaaaagc cgccgcattc aaggaagcct acattgctga gttcgaccgc atggaggccg
      241 aactgcgcca gaataacacc ccacctgctg acaagatgat tccgggtgat ggtcgcactc
      301 tggttgttca cttcgacaaa ttcggcaatg tcgaattcac cgaaaccgtt cctgatggcg
      361 cactggtctg taccctggag actttccggt tttatctgga aaaacaggga tggactcttg
      421 ttaaccgggg cgcaattaaa aatatgactg tggaacaatt actttc
//
LOCUS       DAJVPP010000334          465 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVPP010000334 DAJVPP010000000
VERSION     DAJVPP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.345"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..408
                     /locus_tag="OQM34_004259"
     CDS             <1..408
                     /locus_tag="OQM34_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCS1454433.1"
                     /translation="KVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTS
                     VASGPRALSRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREV
                     KNAMRRWRYEPGKPGSGIVVNILFKINGTTEIQ"
BASE COUNT          132 a          118 c          128 g           87 t
ORIGIN      
        1 aaggtacagg agcagccaaa acgcgatgtc aaacccgtag agtcgcgtcc ggcatcaccg
       61 tttgaaaata cggcacccgc gcgccccaca tcaagtacgg caacggctgc aaccagcaag
      121 ccggttacca gtgtggcttc aggaccacgc gcattaagcc gtaatcagcc gcagtatccg
      181 gcacgagcac aggcattgcg cattgaaggg caggttaaag ttaaatttga cgtcacgccg
      241 gatggtcgcg tggataacgt acaaatcctc tcagccaagc ctgcgaacat gtttgagcgt
      301 gaggtgaaaa atgcgatgcg cagatggcgt tatgagccgg gtaagccagg cagtgggatt
      361 gtggtgaata tcctgtttaa aattaacggc accaccgaaa ttcagtaagc agaaagtcaa
      421 aagcctccga ccggaggctt ttgactatta ctcaacaggt aaggc
//
LOCUS       DAJVPP010000335          459 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVPP010000335 DAJVPP010000000
VERSION     DAJVPP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.346"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(3..>459)
                     /gene="rspA"
                     /locus_tag="OQM34_004260"
     CDS             complement(3..>459)
                     /gene="rspA"
                     /locus_tag="OQM34_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCS1454434.1"
                     /translation="QHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRATLTHAGGITGM
                     RRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPH
                     NWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGTLWNR"
BASE COUNT          110 a          116 c          123 g          110 t
ORIGIN      
        1 ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata agcaggttca
       61 tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg tttgtctccc
      121 ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag catttgttcg
      181 gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa gtgcagcgcc
      241 gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg tacctgatac
      301 agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg ggtcagcgtg
      361 gcgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca gatgctgttg
      421 aagacttcac ccactgcgat gggtgtgacg gtatgttgg
//
LOCUS       DAJVPP010000336          454 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVPP010000336 DAJVPP010000000
VERSION     DAJVPP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.347"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            86..445
                     /locus_tag="OQM34_004261"
     CDS             86..445
                     /locus_tag="OQM34_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454435.1"
                     /translation="MPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIEL
                     FRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQ
                     EGLVAPSQEPQDVKKAV"
BASE COUNT          162 a           90 c           87 g          115 t
ORIGIN      
        1 caagacatac ccgtctttag atcgattagc acaaatagcc tacgcaacag ggcgtccaat
       61 agagtggttt atccaaagtc agagcatgcc aatggttgag cataaaaata atcaagagaa
      121 taaaaattct caacctgacc gcagcacatt gaaaactaaa ctgatcgcaa ttgtcgaatt
      181 gttagaggac aaggaactgc attctgcgat tgagttgttc agaaagaaag gatttgaagc
      241 tcttatgcct gagatttttt cttccgatga ttccatccag gctgatttgc aaggtatttc
      301 gcagcaaacc ttacaaacag ccaaaatgct tgaatcatta ccaacagata ctcgtaaaaa
      361 gattttgtca aagtatggta tttatgaaca ggaaggtctt gtagctcctt cacaagaacc
      421 acaagatgta aaaaaagccg tataacaacc atga
//
LOCUS       DAJVPP010000337          453 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVPP010000337 DAJVPP010000000
VERSION     DAJVPP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.348"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..150)
                     /locus_tag="OQM34_004262"
     CDS             complement(<1..150)
                     /locus_tag="OQM34_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS1454436.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLR"
     gene            305..>453
                     /locus_tag="OQM34_004263"
     CDS             305..>453
                     /locus_tag="OQM34_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Stimulates the peptidyltransferase activity of the
                     70S ribosome and enhances dipeptide synthesis with
                     N-formylmethionyl-tRNA and puromycin in vitro, suggesting
                     its involvement in the formation of the first peptide bond
                     of a protein; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1454437.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGL"
BASE COUNT          113 a          110 c          118 g          112 t
ORIGIN      
        1 tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa tgcagggcgt
       61 attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag gggcagtgca
      121 acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta accaccggat
      181 tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata ccacttgccc
      241 gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact acaggaattt
      301 ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca acggcaaact
      361 gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg ctacgctgta
      421 caaaatgcgt ttttctgatg tccgtaccgg gct
//
LOCUS       DAJVPP010000338          448 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVPP010000338 DAJVPP010000000
VERSION     DAJVPP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.349"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>448
                     /locus_tag="OQM34_004264"
     CDS             <1..>448
                     /locus_tag="OQM34_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS1454438.1"
                     /translation="EQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKA
                     EQVDITGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVE
                     SAPASVTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDD"
BASE COUNT          127 a          110 c          134 g           77 t
ORIGIN      
        1 gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa agacgaaagc gccattcagc
       61 ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga cgcggagtac gagcagaccg
      121 agccaggtaa agccgagcag gtcgatatta ccggaaacca tgtggctctt gtccctaaag
      181 gcagagccgg aaatcgttgt gcaattggag acagagacac aatggcaaat caaaagaaaa
      241 actggtggaa ccgcatgcgt gcggccatca agacaggaga tgccgacacc atgaacgaac
      301 tggtggagtc ggctcccgca tcggttacag gagatgaggg ggatttgccg cagggcgtta
      361 atctcaacat caacctgtcc ccgcagcaac cactaccgga caaagcacca gagatgggcg
      421 gagatccaac cggcgacagt gatgatga
//
LOCUS       DAJVPP010000339          446 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVPP010000339 DAJVPP010000000
VERSION     DAJVPP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.350"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>446
                     /gene="phnD"
                     /locus_tag="OQM34_004265"
     CDS             <1..>446
                     /gene="phnD"
                     /locus_tag="OQM34_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1454439.1"
                     /translation="AFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRANGQVFAQT
                     VAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVPGYYVFAKN
                     NISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPE"
BASE COUNT          107 a          140 c          118 g           81 t
ORIGIN      
        1 cgccttcttt gccccggact acgcgggcat tatccagggg atgcgcttca ataaagtgga
       61 tattgcctgg tacggcaacc tgtcggcgat ggaagcggtg gatcgcgcca atggccaggt
      121 cttcgcccag acggtcgcgg cggatggatc gccgggttac tggagcgtgt tgatcgtcaa
      181 caaagacagc ccgatcaaca acctgaacga tctgctggcg aagcggaaag atctcacctt
      241 cggcaatggc gatcctaact ccacctctgg cttcctcgtc cccggttact acgtcttcgc
      301 taaaaacaat atctccgcca gcgacttcaa gcgcaccgtc aacgccgggc atgaaaccaa
      361 cgcgctggcc gtcgccaaca agcaagtgga tgttgccacc aacaacaccg aaaacctcga
      421 caagctgaaa acctccgcgc cggaga
//
LOCUS       DAJVPP010000340          444 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVPP010000340 DAJVPP010000000
VERSION     DAJVPP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.351"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>444
                     /locus_tag="OQM34_004266"
     CDS             <1..>444
                     /locus_tag="OQM34_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HCS1454440.1"
                     /translation="ILPLREGNGPTLFCFHPASGFAWQFSVLSRYLDPQWSIIGIQSP
                     RPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQVAFLGLLDTWPPETQNW
                     QEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGNYADA"
BASE COUNT           94 a          120 c          132 g           98 t
ORIGIN      
        1 attctgccgt tgcgtgaagg taatggcccg acgctgtttt gtttccatcc ggcgtccggt
       61 tttgcctggc agtttagcgt gctctcgcgt tatctcgatc cacaatggtc gattattggc
      121 attcagtcgc cgcgccctca tggacccatg cagacagcga caaacctgga tgaagtctgc
      181 gaagcgcatc tggcaacgct acttgagcaa caaccgcacg gcattgcggc gcggctgcgt
      241 gcccgtggcg aacaggtggc atttcttggt ttgctggata cctggccgcc agagacgcaa
      301 aactggcagg aaaaagaggc taatggtctg gacccggaag tgctggcgga gattaaccgc
      361 gaacgcgagg ccttcctggc ggcacagcag ggaagtactt caacggagtt gtttaccacc
      421 attgaaggca actacgctga tgct
//
LOCUS       DAJVPP010000341          443 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJVPP010000341 DAJVPP010000000
VERSION     DAJVPP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.352"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..443)
                     /locus_tag="OQM34_004267"
                     /pseudo
     CDS             complement(1..443)
                     /locus_tag="OQM34_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          154 a          108 c          100 g           81 t
ORIGIN      
        1 aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa aaaggccaga
       61 tcaaacgcgc tcacagcttg gcgtggtccg ggcaaattgc gcaacacggc aaagtaccag
      121 aagaagattt gcaatattac ggaagatctc aatataaatt gtggaaagct ttcgtagcag
      181 ccagttatcc gaaagtctcg ccagaccgat aaagaagccc aaaacagaag cgaacacaat
      241 acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc gtccgtaggt
      301 gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc cgcgatccag
      361 aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt tttgaaataa
      421 ccaaccaaca atgccgacaa cag
//
LOCUS       DAJVPP010000342          440 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJVPP010000342 DAJVPP010000000
VERSION     DAJVPP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.353"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>440)
                     /locus_tag="OQM34_004268"
     CDS             complement(<1..>440)
                     /locus_tag="OQM34_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454441.1"
                     /translation="LKKKIRVILFIMAICMAMVLLFLYINKDNTKVIYSLKINQTTPG
                     ILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIRLEWSGDKRDLPTAEEEI
                     SRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIYLTR"
BASE COUNT          120 a           89 c           77 g          154 t
ORIGIN      
        1 ttcctggtca ggtatatcat ttttgtataa agctcactgt taattgctga aggcgtttgt
       61 atctcatcga gaacttgcct gccattatgg tattctgacg cataccattt gataatagac
      121 gcctgcacgc gagatatttc ctcttcagca gttggtaaat ctctcttatc tcctgaccat
      181 tccagcctta tttctctgtt cttgatatct gggctggtct gaaaaaatgt agtaattcgc
      241 tgaataacat cttcagtcat tgtggtctga caggcaaaat tattattgct atcacagcta
      301 acaagtatac ctggcgttgt ctggtttatt ttaaggctgt aaatcacttt tgtattgtct
      361 ttattgatat acagaaacaa cagcaccatt gccatacaga tagccataat aaaaaggata
      421 acacgaatct ttttcttcag
//
LOCUS       DAJVPP010000343          437 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVPP010000343 DAJVPP010000000
VERSION     DAJVPP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.354"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>437
                     /gene="cusB"
                     /locus_tag="OQM34_004269"
     CDS             <1..>437
                     /gene="cusB"
                     /locus_tag="OQM34_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCS1454442.1"
                     /translation="SRIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTLTIR
                     KWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALIDTGN
                     EQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSG"
BASE COUNT           91 a          132 c          130 g           84 t
ORIGIN      
        1 aatcgcgaat tcagggtatg gacccggtgt gggtcactgc tgcgatcccg gagtccatcg
       61 cctggctggt gaaagatgcc tcgcagttta ccctcaccgt tccggcgcga ccggataaaa
      121 cactcaccat ccgcaaatgg acgctgctac ctggcgtgga tgccgcgacc cgcacgctgc
      181 agctgcgtct ggaagtcgac aacgccgacg aggcgctaaa accgggcatg aacgcctggt
      241 tgcaactcaa caccgccagc gaaccgatgc tgctcattcc gtcacaggcg ctgattgata
      301 ccggcaacga acagcgggtg attaccgttg atgccgacgg gcgctttgta ccgaaacgcg
      361 ttgctgtttt ccaggcatcg caaggcgtca ccgcattacg ctctggtctg gcggaaggtg
      421 aaaaggtggt ttccggc
//
LOCUS       DAJVPP010000344          435 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVPP010000344 DAJVPP010000000
VERSION     DAJVPP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.355"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
BASE COUNT          125 a           86 c           74 g          150 t
ORIGIN      
        1 gctctgactc gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc
       61 ggtaaaaaaa ttgcctttcg accattacga tacattattg attttggggt ttcagggaag
      121 taatccattt cgactataac tgacaggtca tcacgacgta tgactgcgta tttgctacta
      181 aatagtttct ttattttttc cacgatgccc ccaggtttat aagtacaaac ggttatatcc
      241 acatagagac aaaaatatta atctgataaa tatttatttc acgccgtata tttgattgtt
      301 taatgtgcaa gtacaatgac ttttattttt tgttgtgtat ataatcaaat atatggttat
      361 ttttcaccct gcgtattcag cacgcaacaa aaaacccgcc aaagcgggtt tagtgcgggt
      421 gcgttgagga tgcct
//
LOCUS       DAJVPP010000345          435 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJVPP010000345 DAJVPP010000000
VERSION     DAJVPP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.356"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>435
                     /locus_tag="OQM34_004270"
     CDS             <1..>435
                     /locus_tag="OQM34_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1454443.1"
                     /translation="VDDQKIAHIVKEKLSQPLARHGELACGYVRTDSGCGFLNTDTLE
                     IIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSI
                     QGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRY"
BASE COUNT          136 a           77 c           98 g          124 t
ORIGIN      
        1 tgtagacgat cagaaaatcg cgcatatagt gaaagaaaaa ttatcgcagc cgctagctcg
       61 ccacggtgaa ttggcttgcg gttatgtacg tactgactca gggtgtggtt ttctgaatac
      121 cgatacgctg gaaataatct ataatgatga agaaagttcg gcaacgttgt ttattaatcc
      181 gcaatggaat tcagctttcg atgcgaagtc attatattta aatccagaca aaaatactgt
      241 taacgctttt atacatcagc aagacatcaa tgttctggca caggatgatt accaatcgtt
      301 gtctattcag ggaaacggtg cgctgggaat aacagaaaat agctatattg gtgcacactg
      361 gaatttcaac ggttatgatg cagatgatgt cagtgacagt aatactgacg tcagcgatct
      421 ctattatcgt tatga
//
LOCUS       DAJVPP010000346          434 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVPP010000346 DAJVPP010000000
VERSION     DAJVPP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.357"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..414)
                     /locus_tag="OQM34_004271"
     CDS             complement(<1..414)
                     /locus_tag="OQM34_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCS1454444.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDL"
BASE COUNT          103 a          135 c          115 g           81 t
ORIGIN      
        1 taaatcgacg gtaacaccgg aaaatttcac cagcggatcg ggcgcggcgg tggcggagtg
       61 gcggcgtaaa gcgacgcgca gacgggcctg caattcgcca ataccaaacg gcttactcag
      121 ataatcatcc gctccggcat ccagcgcggc gatcttgtcg ctctcttcgc tgcgtgcgga
      181 aagcacaatc accggcaccg ggctccactg gcgcaggtcg cggataaact caatcccatc
      241 accatcgggc aggccgagat cgagaataat caaatctggc ttacgggttg ccgcttccag
      301 caagccgcgt tgcagagttt cggcctcaaa gacgcgcatc ccgtcgccct ccagcgccgt
      361 gcgcagaaag cgacgaatag cctgttcatc ttcaacaatc agaacgtttg tcacatatcc
      421 tcatgaaagt cttc
//
LOCUS       DAJVPP010000347          432 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVPP010000347 DAJVPP010000000
VERSION     DAJVPP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.358"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>432
                     /locus_tag="OQM34_004272"
     CDS             <1..>432
                     /locus_tag="OQM34_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1454445.1"
                     /translation="ADELWQKLESYPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVL
                     ITATDCNKRWLQEVIHFNVLAIVPRDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDI
                     KDLKSLSARQREILTMLAAGESNKEIGRALNISTGTVKAHL"
BASE COUNT          114 a           84 c          132 g          102 t
ORIGIN      
        1 ggcagacgag ttatggcaaa agctggaaag ttaccctgaa gccttagtta tgctcgatgg
       61 cgatcaggat ggtgagtttt gctactggtt gctgcaaaaa accgtggtgc agtttcctga
      121 ggttaaggtg ttaatcacgg cgacggattg caataaacgg tggttacagg aagttatcca
      181 ttttaatgtg ctggccattg tgcctcgcga ttcaaccgtc gagacatttg cgttggcggt
      241 aaatagtgcg gcgatgggga tgatgtttct gcctggagac tggcggacga cgccggaaaa
      301 ggacattaaa gatctcaaat cgttaagcgc ccgccagcgg gagattttaa ccatgttagc
      361 ggcgggcgaa tcaaataagg agatcggcag agcgctgaat atcagtaccg gaacggtaaa
      421 agcgcatctg ga
//
LOCUS       DAJVPP010000348          432 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVPP010000348 DAJVPP010000000
VERSION     DAJVPP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.359"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            46..285
                     /gene="relB"
                     /locus_tag="OQM34_004273"
     CDS             46..285
                     /gene="relB"
                     /locus_tag="OQM34_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCS1454446.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            285..>432
                     /gene="relE"
                     /locus_tag="OQM34_004274"
     CDS             285..>432
                     /gene="relE"
                     /locus_tag="OQM34_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCS1454447.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPD"
BASE COUNT          120 a           84 c          117 g          111 t
ORIGIN      
        1 gtgcgatact tgtaatgaca tttgtaatta caagaggtgt aagacatggg tagcattaac
       61 ctgcgtattg acgatgaact taaagcgcgt tcttacgccg cgcttgaaaa aatgggtgta
      121 actccttctg aagcgcttcg tctcatgctc gagtatatcg ctgacaatga acgcttgccg
      181 ttcaaacaga cactcctgag tgatgaagat gctgaacttg tggagatagt gaaagaacgg
      241 cttcgtaatc ctaagccagt acgtgtgacg ctggatgaac tctgatggcg tattttctgg
      301 attttgacga gcgggcacta aaggaatggc gaaagctggg ctcgacggta cgtgaacagt
      361 tgaaaaagaa gctggttgaa gtacttgagt caccccggat tgaagcaaac aagctccgtg
      421 gtatgcctga tt
//
LOCUS       DAJVPP010000349          431 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVPP010000349 DAJVPP010000000
VERSION     DAJVPP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.360"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..430
                     /locus_tag="OQM34_004275"
     CDS             <1..430
                     /locus_tag="OQM34_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454448.1"
                     /translation="LKALLAKLERNATGDNDNKPDDNPTGDGEDDEEETTITGDSAWR
                     AEVIVPGIDLSRKMKPTAFKREVLASADKTLVRQIVGDADIRKLPKQSVDMAFNAVSE
                     VAKGRNTRATTGDAQRLNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          123 a          110 c          119 g           79 t
ORIGIN      
        1 actgaaagcc ctgctggcta agctggaaag aaatgccacg ggcgataacg ataataagcc
       61 tgacgataat ccgaccggtg acggcgagga cgatgaagag gaaaccacga ttactggtga
      121 ctcagcctgg cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta agatgaaacc
      181 gaccgcgttc aaacgcgagg ttctggcttc tgctgacaaa acgctggttc gccagatagt
      241 cggtgatgcg gatatccgca aattaccgaa acaatcggtc gacatggcgt ttaatgccgt
      301 gtctgaggtt gccaaagggc gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa
      361 catgggcatg accagtatcg cttccctgaa caaacaaaac gctgaattct gggcaaaccg
      421 taaagggtaa a
//
LOCUS       DAJVPP010000350          430 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVPP010000350 DAJVPP010000000
VERSION     DAJVPP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.361"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>430)
                     /locus_tag="OQM34_004276"
     CDS             complement(<1..>430)
                     /locus_tag="OQM34_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1454449.1"
                     /translation="YRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNLNDILLPQFQQ
                     AFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYALGAALFWPAA
                     EIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSH"
BASE COUNT          141 a           91 c           92 g          106 t
ORIGIN      
        1 aagtgactac tgctttccgg ccctaatacc gtaacaaaag ggtttgcggc agtttccaga
       61 caacctaatc cggctgcaat aataaatagg ccaactaaaa ataaggtgta gttcattatt
      121 tctgcagcgg gccagaataa tgcagcgccc aaggcatata aaaataatcc ggtaataatc
      181 cctgctttat aactgagttt tttcatcaat atcccagcag ggattgggat aatgaaataa
      241 ccaaagtaaa aggccgattg gatcaggcca gcctggaaat ttgtcagcgt aaaagcctgc
      301 tggaattgag gtaataaaat gtcgttaagg ttattggcta ccgcccaaag aaaaaacagt
      361 gagcacagca gcgcgaatgg aataatgtaa cttctgcttt gccctgcatc tttatctacc
      421 gcacggtaac
//
LOCUS       DAJVPP010000351          429 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVPP010000351 DAJVPP010000000
VERSION     DAJVPP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.362"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..312
                     /locus_tag="OQM34_004277"
     CDS             <1..312
                     /locus_tag="OQM34_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCS1454450.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT           76 a          111 c          109 g          133 t
ORIGIN      
        1 gtggctttta tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc
       61 gcatggcaac gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca
      121 ctggcctggg gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc
      181 ggtttgccca tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc
      241 tttgaactgc gtcatttata tcaactcatc acgcgtagca attctctaac gtcgccaccg
      301 caaggagctt aatcatggct gtgctgattt ttctgggctg tctgttgggt ggtattgcta
      361 tcggtttgcc tattgcctgg gcactgttgt tgtgcggggc ggcgttaatg ttctggctgg
      421 acatgtttg
//
LOCUS       DAJVPP010000352          427 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVPP010000352 DAJVPP010000000
VERSION     DAJVPP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.363"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(21..>427)
                     /locus_tag="OQM34_004278"
     CDS             complement(21..>427)
                     /locus_tag="OQM34_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="YdeF; uncharacterized member of the major
                     facilitator superfamily (MFS) of transporters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCS1454451.1"
                     /translation="YSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLLLWGMSA
                     AVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVLTSLPPS
                     SLFVILALVIIAAWVLMLKGIRARPWGQPALC"
BASE COUNT          123 a          116 c          104 g           84 t
ORIGIN      
        1 tttattgtgt tcgacttaaa tcaacaaagc gcgggctgcc cccacggtct tgcacgaatc
       61 ccttttaaca tcagcaccca cgcagcaatg atcaccaacg ctaagatgac aaacagcgag
      121 gaaggcggca ggctggttag cactacgcca ctcactaatg ggttaatcgc ggcaccaagc
      181 cagcctaaag actgggcgga aaaatagctg gctttcattc ctggcggcgc aatatggtca
      241 atcaacatat actcgcccgg cgcataaatg atttcaccga cagtaaatac cgcagctgac
      301 ataccccaca atagcaggtt gttgccggaa aaaataaaac cgaccagtcc gatgacgaaa
      361 cagagggtgc ctgctgtcat cagcgcgcgg atgttagctg ggttaagtcg gcggcccacg
      421 gaatatt
//
LOCUS       DAJVPP010000353          426 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJVPP010000353 DAJVPP010000000
VERSION     DAJVPP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.364"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..426
                     /locus_tag="OQM34_004279"
                     /pseudo
     CDS             1..426
                     /locus_tag="OQM34_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          112 a          111 c          118 g           85 t
ORIGIN      
        1 attgaaatgg aaaatgaaat attaaaaagg ctaccgcgct cttgatgtca gactccctga
       61 acagttttcg ataatcggga aactcagggc gcgttatcct gtggccactc tctgccatgt
      121 gttcggggtc catcgcagca gctacaaata ctggaaaaac cgtcctgaaa aaccagacgg
      181 cagacgggct gtattacgca gccaggtact tgaactgcat ggcatcagcc acggctctgc
      241 cggagcaaga agcatcgcca caatggcaac ccagagaggc taccagatgg ggcgctggct
      301 tgctggcaga ctcatgaaag agctggggct ggtcagttgc cagcagccga ctcaccggta
      361 taagcgtggc ggtcatgagc acgttgctat cccgaatcat cttgagcgac agttcgccgt
      421 aacgga
//
LOCUS       DAJVPP010000354          426 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJVPP010000354 DAJVPP010000000
VERSION     DAJVPP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.365"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>426)
                     /locus_tag="OQM34_004280"
     CDS             complement(<1..>426)
                     /locus_tag="OQM34_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1454452.1"
                     /translation="KIKELPFLPENLTHLRVHNNRLHILPLLPPELKLLVVSGNRLDS
                     IPPFPDKLEELALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNAFVNVAG
                     NPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSATISAPEH"
BASE COUNT          123 a           72 c           98 g          133 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattc gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagctcttc aagcttatct ggaaaggggg gaatgctgtc
      301 taatctgttt ccagaaacta ccagtaattt tagttccggt ggcaatagtg gcagaatatg
      361 caatcggtta ttatgaaccc gtaaatgagt tagattttca ggaagaaatg gtaattcttt
      421 tatctt
//
LOCUS       DAJVPP010000355          414 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVPP010000355 DAJVPP010000000
VERSION     DAJVPP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.366"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..364)
                     /locus_tag="OQM34_004281"
     CDS             complement(<1..364)
                     /locus_tag="OQM34_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1454453.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
BASE COUNT          144 a           99 c           78 g           93 t
ORIGIN      
        1 ccagaccgat acgcattaaa ataaagtggc taaaagaatt aaccattcct gttaatgcct
       61 gcattaatga ccagaatatt aagcctgcgc ctaaaactat tcgtgggcca aagcggtcta
      121 gcaaaatacc ggaaggtagt tgtgaaaaac cataagacag agaaaatgca gaaagcaaaa
      181 caccaaactc tgtcgccgat aatcctaact cgccacgaat cgcttcgcct gccacgctca
      241 atgacgaacg gtcgagaaaa ttaacgatcc ccgccataaa taataaaacc agggtcactg
      301 tctgaatacg acgaatccgt tgcggtttat tacccgcaac actggttgac acatcaacgt
      361 ccatttttag ctccttgagc ggaattaatc gatttgatga ctattcagaa aata
//
LOCUS       DAJVPP010000356          408 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVPP010000356 DAJVPP010000000
VERSION     DAJVPP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.367"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..111
                     /locus_tag="OQM34_004282"
     CDS             <1..111
                     /locus_tag="OQM34_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1454454.1"
                     /translation="LKDSPSQPLQQSLKDLERAYKNFFRKRAAFPRFKKR"
     gene            193..>408
                     /locus_tag="OQM34_004283"
     CDS             193..>408
                     /locus_tag="OQM34_004283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1454455.1"
                     /translation="MRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPAHPSAS
                     MVGLDAGVAKLATLSDGTVFEPVNSFQK"
BASE COUNT          104 a          106 c          103 g           95 t
ORIGIN      
        1 cttaaagatt ctccctcaca gccattgcaa cagtcactga aagaccttga gcgggcttac
       61 aaaaacttct tccggaagcg ggctgctttt ccccgattca aaaagcggtg acagaatgat
      121 gcattccgct acccgcaggg cgttaagctc gatcaggaaa acagccgtat ttttctgccg
      181 aaactgggct ggatgcgcta ccggaatagc cgtcaggtca cgggtgttgt gaaaaatgtc
      241 actgtcagcc agtcctgcgg tacgtggtac atcagtattc agacagaaag tgaagtatcc
      301 actccggctc acccttcagc atcaatggtc gggctggatg ctggcgtggc taaactcgcc
      361 acgctgtcag atggcacagt ctttgagcct gtaaacagtt ttcagaaa
//
LOCUS       DAJVPP010000357          404 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJVPP010000357 DAJVPP010000000
VERSION     DAJVPP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.368"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>404)
                     /locus_tag="OQM34_004284"
     CDS             complement(<1..>404)
                     /locus_tag="OQM34_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCS1454456.1"
                     /translation="LREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGLLGILLGVAVI
                     IVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEMVPAFKPMAPAATSLVA
                     TAVIVTLILVPILTSIWSRKVKARAAKIEILG"
BASE COUNT          113 a          113 c           92 g           86 t
ORIGIN      
        1 acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga tagaggtaag
       61 aattggcacc agaatcaaag tcacaatgac cgccgtcgct accagcgaag ttgctgccgg
      121 agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc
      181 ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa tcaatttatc
      241 ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca ggatccccag
      301 caaaccagtc tgggcaatca cagtcaaatc aatggtattg cccagcgcga aggcaaagaa
      361 tggaatcagc gtttgcaccg ctttgctgaa aaattctcgt aatt
//
LOCUS       DAJVPP010000358          403 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVPP010000358 DAJVPP010000000
VERSION     DAJVPP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.369"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>403)
                     /locus_tag="OQM34_004285"
     CDS             complement(<1..>403)
                     /locus_tag="OQM34_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCS1454457.1"
                     /translation="LPHKFLCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLG
                     GPVVGGLVGLTGGLHRYSMGGMTALSCMISTIVEGLLGGLVHSILIRRGRTGKVFNPI
                     TAGAVTFVAEMVQMLIILAIARPYEDAVRLVS"
BASE COUNT          108 a          116 c          108 g           71 t
ORIGIN      
        1 tactcaccag acgcaccgca tcttcataag gtcgggcgat cgcaaggatg atcagcattt
       61 gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag actttaccag
      121 tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaatcct tcaacgatgg
      181 ttgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt aagccgccgg
      241 tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt accgcgccta
      301 tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg cccatgatgc
      361 agaagatgga aaagacgatg tagcagagaa atttatgcgg cag
//
LOCUS       DAJVPP010000359          397 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVPP010000359 DAJVPP010000000
VERSION     DAJVPP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.370"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(19..397)
                     /locus_tag="OQM34_004286"
                     /pseudo
     CDS             complement(19..397)
                     /locus_tag="OQM34_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT          143 a           67 c           90 g           97 t
ORIGIN      
        1 gcatttttta tggtggagtt accagcggcc ggatgcgccg ccacttgagc ctccagcgga
       61 accgctgttg tctgaatgat aacttctacc agatgctctt ttctgaagta cacataaaca
      121 ggtaaagacc ataaatatgg tgaaagtaaa aaagaaggaa aatacagcaa tggaaaatgt
      181 tttatcggag aacaggaata aatctaaaag atggattgct gaaatacaga ctccactttt
      241 acatgcgcga caaaaattac tgccttgatg aaaaaactag aaagggaaga acatcactgc
      301 aaatacccaa aaaatagcaa agtaataatg atcagtggaa gacgttgatt cactttctga
      361 aggattagcc ggatattggt gttgtgaagt gagttgg
//
LOCUS       DAJVPP010000360          395 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVPP010000360 DAJVPP010000000
VERSION     DAJVPP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.371"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..73
                     /locus_tag="OQM34_004287"
     CDS             <1..73
                     /locus_tag="OQM34_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1454458.1"
                     /translation="SARFNSAMDIPAQGLCRIPEKRS"
     gene            83..>395
                     /locus_tag="OQM34_004288"
     CDS             83..>395
                     /locus_tag="OQM34_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1454459.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NF"
BASE COUNT           90 a           93 c          119 g           93 t
ORIGIN      
        1 gtcggctcgt tttaattctg ccatggacat tcccgctcag gggctgtgcc gtattccgga
       61 aaaacgttct taaggaggga agatgcgcca ttgtgggtgg ttgctgggat tgttatcgct
      121 gttttctttg gcaacacatg ccagtgactg gcaggaaatt aaaaatgagg ccaaagggca
      181 aaccgtctgg tttaacgcct ggggcggcga taccgcaatt aaccgctatc tcgactgggt
      241 tagcggcgag atgaaaaccc attacgctat aaacctgaag attgttcgtc tcgcggatgc
      301 cgcagacgcg gtgaagcgca ttcagaccga agctgctgcc ggtcgtaaaa cgggcggctc
      361 ggtggatctg ctctgggtga acggcgaaaa cttcc
//
LOCUS       DAJVPP010000361          394 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVPP010000361 DAJVPP010000000
VERSION     DAJVPP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.372"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..392
                     /locus_tag="OQM34_004289"
     CDS             <1..392
                     /locus_tag="OQM34_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1454460.1"
                     /translation="PRERKLVSGFVDYLCESVGREHTPALSDAAEQYLNTVATCRTRD
                     TALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDETEFQQVYKSVLNV
                     LWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT           98 a           90 c          106 g          100 t
ORIGIN      
        1 cgcctcgcga acgaaaactg gtatcaggct tcgttgatta cctgtgcgaa tcagtaggtc
       61 gggaacacac tccagccctg agtgatgccg cagagcaata ccttaataca gttgcgacat
      121 gcagaacccg ggatacggca ttgctaaagt cgtttgacgc tttccgcgag tgggtaacca
      181 ttcaggccgg attttacacc gagcatattt atcctgatgg tagtcgtggg cgcagggcaa
      241 aatctatcgc atttgcgaac atggacgaaa ccgagtttca gcaggtttat aaatctgtac
      301 tgaatgtgct gtggaactgg atcctgttcc gtaaattttc ctctccggag gaagtcgaaa
      361 atgtggccgc gcagttactg gagtttgcgt aatg
//
LOCUS       DAJVPP010000362          394 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVPP010000362 DAJVPP010000000
VERSION     DAJVPP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.373"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
BASE COUNT          106 a           79 c           93 g          116 t
ORIGIN      
        1 tttattttct gaatcacacc aaaaacaacg aaacaataat taaaaaagga ttctgttgtt
       61 gttaccggga accgacttat ttcgctcaga aagtcgttca accagctccg cagtgataag
      121 attcgctgat gatacagtgt atcctcactg cgtgctggag gtgccagtat aatatgttga
      181 tcaatacagt atttattgtg ttcagtcgtt tcctgatgcg tttggttctc tgcggattgt
      241 cgctggcgaa ataaatggct tgcggtaaat gccgttacag agcagcgcaa cgcgtcaatt
      301 ccttcggagt gcccggcttt tggcgagagt tccggtttta tgcgtccagg tagcggaaat
      361 ttcacccgaa tgagaaatgc attaataaaa tgga
//
LOCUS       DAJVPP010000363          392 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJVPP010000363 DAJVPP010000000
VERSION     DAJVPP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.374"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..392)
                     /locus_tag="OQM34_004290"
                     /pseudo
     CDS             complement(1..392)
                     /locus_tag="OQM34_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          111 a           80 c           79 g          122 t
ORIGIN      
        1 ggagttgatt gtgtgttatt tacgaagtta ttcatcggtt cttggaagtt agcaatttta
       61 ttagttgtac cattaaacca gtcagcgata gaactattgg cttagatgaa tcacctttgt
      121 attcagggtt gaagtttgtt tctgtgccaa tacctgactg atgtaagttc ggattcttag
      181 ctgcttcagg gtttaaatta tcaagagcaa aatttaccaa ctcagcataa aggacaactc
      241 taccgacttt atttagcgca ccacctgaag aaccagcctt gccagcttta gctttattaa
      301 cagcgtcacc gaatgaagta acagcgtttg caaggtttaa caagcctcct gctacatttg
      361 cagccttttg agcagtaaca gccacagcaa gc
//
LOCUS       DAJVPP010000364          392 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVPP010000364 DAJVPP010000000
VERSION     DAJVPP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.375"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            278..>392
                     /locus_tag="OQM34_004291"
     CDS             278..>392
                     /locus_tag="OQM34_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1454461.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLI"
BASE COUNT          110 a          109 c           64 g          109 t
ORIGIN      
        1 tagctgttta tttatacagc aaatcctgtt tcggacttct cactccccat ttgatcaact
       61 gaagctgaac ccgttttttt ctgcatgcct caatcgtcaa aattcttcgc caccagccag
      121 ctctgccata acttgccaca ttgaaaaagt agccaaagtg cttcaggcac catcagttca
      181 ccatcaccaa aaaaaagaaa cattccagaa cttccgcatc tcgcccagcc gctccgactt
      241 ctatactgaa tagaaaacgt caacttaaga gaaacctatg ccattacccg attttcatgt
      301 ttctgaacct tttaccctcg gtattgaact ggaaatgcag gtggttaatc cgccgggcta
      361 tgacttaagc caggactctt caatgctgat tg
//
LOCUS       DAJVPP010000365          391 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVPP010000365 DAJVPP010000000
VERSION     DAJVPP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.376"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            126..>391
                     /gene="mcbR"
                     /locus_tag="OQM34_004292"
     CDS             126..>391
                     /gene="mcbR"
                     /locus_tag="OQM34_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCS1454462.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIR"
BASE COUNT          117 a           78 c           96 g          100 t
ORIGIN      
        1 acgtcattgt tctgctgcta ataacaacaa aggtgggtgg tttatgatta ttcactttaa
       61 tacactaggt gaactccttc tgccatgcag gcagggttgg acagaaaaca cgcgaaggaa
      121 ttaaaatgcc tggaacggga aaaatgaaac atgtcagttt gactctgcag gttgagaacg
      181 acctgaaaca tcagcttagt attggcgcat taaaacctgg cgcacgcctg attactaaaa
      241 atctggcgga gcaattaggt atgagtatta cacctgtgcg tgaagcatta ttacgtctgg
      301 tttcggtgaa tgcgctttct gtcgcacctg cacaagcatt tacagtaccg gaagtgaggc
      361 aaagtcagtt agacgaaatc aatcggatcc g
//
LOCUS       DAJVPP010000366          390 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVPP010000366 DAJVPP010000000
VERSION     DAJVPP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.377"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..361
                     /locus_tag="OQM34_004293"
     CDS             <1..361
                     /locus_tag="OQM34_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCS1454463.1"
                     /translation="RRYAEIADHLGLSAPGDRTAAKIEKLLAWLETLKAELGIPKSIR
                     EAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQILLDTYYGRDYVEGETAA
                     KKEAAPAKAEKKAKKSA"
BASE COUNT          109 a           95 c          106 g           80 t
ORIGIN      
        1 ccgtcgttat gctgaaattg ccgaccacct gggtctgagc gcaccgggcg accgtactgc
       61 tgctaagatc gagaaactgc tggcatggct ggaaacgctg aaagctgaac tgggtattcc
      121 gaaatctatc cgtgaagctg gcgttcagga agcagacttc ctggcgaacg tggataaact
      181 gtctgaagat gcattcgatg accagtgcac cggcgctaac ccgcgttacc cgctgatctc
      241 cgagctgaaa cagattctgc tggataccta ctacggtcgt gattatgtag aaggtgaaac
      301 tgcagcgaaa aaagaagccg ctccggctaa agctgagaaa aaagcgaaaa aatccgctta
      361 atcagtagcg ctgtctggca atataaacgg
//
LOCUS       DAJVPP010000367          390 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVPP010000367 DAJVPP010000000
VERSION     DAJVPP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.378"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>390)
                     /locus_tag="OQM34_004294"
     CDS             complement(<1..>390)
                     /locus_tag="OQM34_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454464.1"
                     /translation="SASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMM
                     TKTRAWALDAETAPVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAI
                     NQAELLANDLTAVGFYRLAHVLAQFRN"
BASE COUNT          103 a          106 c          102 g           79 t
ORIGIN      
        1 tattacgaaa ttgtgccaac acatgtgcca gccgataaaa acccaccgcg gtgaggtcat
       61 tcgccagcaa ctctgcctga ttaatagcgc tctgttcctg atagcgccag ccgttatgga
      121 gcagttgaat aagtaacgcc tggcagcgca tcagcaactg atgagcagta gacggcacag
      181 gcagaacgct ggcagaaggt agtgatacca ccacaggcgc agtttctgcg tccagcgccc
      241 aggcacgagt ctttgtcatc attacctgtg gttccagtgt caattgccca tcaacaaaac
      301 tgacaaagcc agaaaccaga cacacggggt cgtctgtttg ttgcaaaagc gccgccatgc
      361 gttcaatggc ataaggcgcg ctggctgagg
//
LOCUS       DAJVPP010000368          389 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJVPP010000368 DAJVPP010000000
VERSION     DAJVPP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.379"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            29..>389
                     /gene="ccp"
                     /locus_tag="OQM34_004295"
     CDS             29..>389
                     /gene="ccp"
                     /locus_tag="OQM34_004295"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1454465.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVM"
BASE COUNT          106 a           82 c           97 g          104 t
ORIGIN      
        1 aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta ccgcgatcgg
       61 cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt accacgataa
      121 taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata ataaggtttt
      181 aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag aattacccgc
      241 ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc ttggatataa
      301 atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg tttcgcaaag
      361 cgatctgaat aagattgaat gggtgatgc
//
LOCUS       DAJVPP010000369          383 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVPP010000369 DAJVPP010000000
VERSION     DAJVPP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.380"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..375
                     /locus_tag="OQM34_004296"
     CDS             <1..375
                     /locus_tag="OQM34_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1454466.1"
                     /translation="SLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKIDVRLTLNAHGI
                     KRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGLGLSIVQRIAK
                     LHDMNVEFGNAEQGGFEAKVSW"
BASE COUNT           95 a           98 c          108 g           82 t
ORIGIN      
        1 tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct acaatcgtcg
       61 gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat
      121 gcccacggca tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg
      181 ctggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
      241 cgcccacccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
      301 gccaagctgc atgacatgaa tgttgagttt gggaatgcgg aacaaggtgg atttgaggcg
      361 aaggtaagct ggtaacttct ctg
//
LOCUS       DAJVPP010000370          382 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJVPP010000370 DAJVPP010000000
VERSION     DAJVPP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.381"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(166..>382)
                     /locus_tag="OQM34_004297"
     CDS             complement(166..>382)
                     /locus_tag="OQM34_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1454467.1"
                     /translation="AKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDE
                     IYRRQREMKEELSGLDDLASIRAFDVE"
BASE COUNT           74 a          130 c           85 g           93 t
ORIGIN      
        1 cacagaccgg actgactccg gaaaaacgga ggcccgcccc ccgggcctcc cctgattcat
       61 ccgtttccct gttcagcctg acagcacgcc ccggctgccg gatgacagac tccgcttcgg
      121 taagcaaagc ggtcttctgt gattccgcca gttgcggctt attcattact caacgtcaaa
      181 cgcccgaatt gaagccaaat catccagacc gctcagctcc tctttcattt cacgctgacg
      241 gcgataaatc tcatcgttgc gatcgacctg cgcctgcacc attgctgccg ccagttcttc
      301 cagttccggc atcgacagtt tcacctgctg attatcggca tcgctccacg ccatatgtgt
      361 ttgtgctgtg acagattttg cc
//
LOCUS       DAJVPP010000371          381 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJVPP010000371 DAJVPP010000000
VERSION     DAJVPP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.382"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            25..>381
                     /gene="treF"
                     /locus_tag="OQM34_004298"
     CDS             25..>381
                     /gene="treF"
                     /locus_tag="OQM34_004298"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072205.1"
                     /note="cytoplasmic; catalyzes the hydrolysis of trehalose
                     to glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCS1454468.1"
                     /translation="MIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADR
                     YLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLP
                     EVYSSEYVSDPQNSLKE"
BASE COUNT           99 a          101 c           92 g           89 t
ORIGIN      
        1 caaaacccta atccagacga actgatgatc gaggtcgatc tctgctatga actggacccg
       61 tatgaattaa aactggatga gatgatcgag gcagaaccgg aacccgagat gattgaaggg
      121 ctgcccgcct ctgatgcgct gacgcctgcc gatcgctatc tcgaactgtt cgagcatgtt
      181 cagtcggcga aaattttccc cgacagtaaa acctttcccg actgcgcacc caaaatggac
      241 ccgctggata ttttaatccg ctaccgtaaa gtgcgccgtc atcgtgattt tgacttgcgc
      301 aagtttgttg aaaatcactt ctggctgccg gaggtctact ccagcgagta tgtatcggac
      361 ccgcaaaatt ccctgaaaga g
//
LOCUS       DAJVPP010000372          378 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJVPP010000372 DAJVPP010000000
VERSION     DAJVPP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.383"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..308
                     /locus_tag="OQM34_004299"
     CDS             <1..308
                     /locus_tag="OQM34_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454469.1"
                     /translation="LRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLN
                     MQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
BASE COUNT          101 a           72 c          103 g          102 t
ORIGIN      
        1 atcttcgcga tgaagttatg cgcctggacg cagggatcac gcaggaagta ttgaagctat
       61 atattgcgtt taaagcggaa acgaattttg ttgatgttgt gccgcaaaaa agccgactgc
      121 gattgtcgct taatatgcag tttcatgaac tggtcgatcc gaaaggtatt gccaaagatg
      181 tgacaaatgt tgggcgctgg ggtaatggcg atgtagaaat tggtttcagc gacctcgcac
      241 aacttcctta cattatggga ttaattcgtc aggcatttga aaaacagatg gagagcgcgt
      301 tggtataacg cggcgaataa tacccggtag cactggcgct accgggtcaa tgtatcaggc
      361 gactttctgt ttacctgt
//
LOCUS       DAJVPP010000373          378 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVPP010000373 DAJVPP010000000
VERSION     DAJVPP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.384"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>378)
                     /locus_tag="OQM34_004300"
     CDS             complement(<1..>378)
                     /locus_tag="OQM34_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1454470.1"
                     /translation="AITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAAITVS
                     QRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLANM
                     MFGSISGSAVAAAAAVGGTLNPI"
BASE COUNT          130 a           96 c           91 g           61 t
ORIGIN      
        1 gaatgggatt cagtgtgcca ccaacggcag cagctgccgc aacggctgaa ccagaaatgg
       61 aaccaaacat catattcgcc aggacgttca catgaccaag cgaaccagga acgcggccaa
      121 ccattacctg ggcgaggtta atcaaacgta tcgcaatacc gccgctgttc atcagtgttc
      181 cggcaaagat gaaaaagggg attgccaaca gggcaaagtt atccagtcca ttagccagtc
      241 gttgcgaaac agtgattgcc gcgatatccc agggaaacat caaaagcatt gaagctacag
      301 tggcaatacc gattgagaac gaaatgggca caccaataaa gaccagcaca aaaaaacagc
      361 caaacagtgt aatcgcga
//
LOCUS       DAJVPP010000374          374 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVPP010000374 DAJVPP010000000
VERSION     DAJVPP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.385"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..167)
                     /locus_tag="OQM34_004301"
     CDS             complement(<1..167)
                     /locus_tag="OQM34_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1454471.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMK"
BASE COUNT           91 a           89 c           89 g          105 t
ORIGIN      
        1 ttcttcatga aactgcggtg ttcgagtttg cgatatttct tacaggggaa cgcaagcccc
       61 tccgcctccg ctttggattt ccgatcagca ttaatgtaat ccaccatctc aagactgctc
      121 atggtcgggt attcgccaga aggaagaacg gttaatgatt tgttcatgtc ggtatctcct
      181 ttagaaagtt gagcctgttc gcacagaaaa gccgtccccg agaggtcctc acctatacgg
      241 cagttctcag gctcagcttt ctgaaagact cgggattgtt acgcgctgcg atgcgcggtt
      301 tactgcggac atataaaagc cccgcaagtg cgaggctcat taaatggact ttgtgatttg
      361 caaaaaaatt tatt
//
LOCUS       DAJVPP010000375          372 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVPP010000375 DAJVPP010000000
VERSION     DAJVPP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.386"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(33..>372)
                     /locus_tag="OQM34_004302"
     CDS             complement(33..>372)
                     /locus_tag="OQM34_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1454472.1"
                     /translation="EPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDER
                     DALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTITDKSSYNAI
                     EWPASPEGSS"
BASE COUNT           87 a           95 c           89 g          101 t
ORIGIN      
        1 tacagacatt gtttttctcg cgataaatta aatcaggaag aaccttccgg agaggcgggc
       61 cattcaatgg cgttgtatga ggatttatca gtgatggtgc tgaaatccat cgcctgcagc
      121 gatttcgcgt aaatacgaca ggctttcagc ttttctttat cttcgtcgct gattaacccc
      181 agcagcaggt cttcttccca ttcccctgtc cgggcactga cctgagccag aagggcatca
      241 cgctcatctt ccgctttgag tctgtagtca aagacaaatt catcattgcg gtaaaaccag
      301 taacccggcg cggtaatccg tcggttagcg gtaatatccg gaacttcgat aacgctggca
      361 ttgcgtggct cg
//
LOCUS       DAJVPP010000376          372 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVPP010000376 DAJVPP010000000
VERSION     DAJVPP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.387"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..311)
                     /locus_tag="OQM34_004303"
     CDS             complement(<1..311)
                     /locus_tag="OQM34_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1454473.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJVPP010000377          369 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVPP010000377 DAJVPP010000000
VERSION     DAJVPP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.388"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..122
                     /locus_tag="OQM34_004304"
     CDS             <1..122
                     /locus_tag="OQM34_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS1454474.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..>369
                     /locus_tag="OQM34_004305"
     CDS             138..>369
                     /locus_tag="OQM34_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1454475.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDY"
BASE COUNT          114 a           72 c           87 g           96 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacg
//
LOCUS       DAJVPP010000378          365 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVPP010000378 DAJVPP010000000
VERSION     DAJVPP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.389"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>365
                     /locus_tag="OQM34_004306"
     CDS             <1..>365
                     /locus_tag="OQM34_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1454476.1"
                     /translation="LLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAIC
                     EQIGDPATSKGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDIS
                     SGRFRLSEPADRETMAAEL"
BASE COUNT           83 a          100 c          115 g           67 t
ORIGIN      
        1 aactgctgga tatttcactg accaaacgcg gtgcttcggc gggagagccg atcccgatgg
       61 cggggattcc ctaccatgcg gtggaaaact atctcgccaa actggtgaat cagggagagt
      121 ccgttgccat ctgcgaacaa attggcgatc cggcgaccag caaaggtccg gttgagcgca
      181 aagttgtgcg tatcgttacg ccaggcacca tcagcgatga agccctgtta caggagcgtc
      241 aggacaacct gctggcggct atctggcagg acagcaaagg tttcggctac gcgacgctgg
      301 atatcagctc cgggcgtttt cgcctgagcg aaccggccga ccgcgaaacg atggcggcag
      361 agctg
//
LOCUS       DAJVPP010000379          359 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVPP010000379 DAJVPP010000000
VERSION     DAJVPP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.390"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>359
                     /locus_tag="OQM34_004307"
     CDS             <1..>359
                     /locus_tag="OQM34_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1454477.1"
                     /translation="AAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHG
                     KPWYAAHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAE
                     EMQILREIFNSGWQHNW"
BASE COUNT           96 a           84 c           99 g           80 t
ORIGIN      
        1 cgcagcggaa gcgtggttga agtcacaagg tgcaagtaag atcagcgggc ctttcagcct
       61 gaatatcaat caggaaagcg gattactgat tgaaggtttt gacacaccac cctgtgcgat
      121 gatgccacac ggcaaaccgt ggtatgccgc gcatattgaa caactgggtt atcacaaagg
      181 cattgattta ctggcgtggt ggatgcagcg aaccgatctc actttctctc cggcgctaaa
      241 aaaactgatg gatcaggtgc gcaaaaaggt gaccattcgc tgcatcaatc gtcagcggtt
      301 tgccgaagag atgcagatcc tgcgtgagat tttcaactct ggctggcagc acaactggg
//
LOCUS       DAJVPP010000380          358 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJVPP010000380 DAJVPP010000000
VERSION     DAJVPP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.391"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..>358
                     /locus_tag="OQM34_004308"
     CDS             1..>358
                     /locus_tag="OQM34_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="YhcL; uncharacterized member of the DcuC family of
                     anaerobic dicarboxylate transporters; probable role in
                     transport of carboxylates across the inner membrane from
                     the periplasm; its encoding gene is not essential for
                     growth and is probably cryptic; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter"
                     /protein_id="HCS1454478.1"
                     /translation="MIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGV
                     LDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSLLSR
                     PLKLIRSPYIILSATYV"
BASE COUNT           76 a           84 c           98 g          100 t
ORIGIN      
        1 ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa aaactacaaa
       61 cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt ctggttaggg
      121 ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga catttataat
      181 gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat tatggcggtg
      241 ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt gagcttgcta
      301 agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac ttacgtca
//
LOCUS       DAJVPP010000381          357 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVPP010000381 DAJVPP010000000
VERSION     DAJVPP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.392"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(25..297)
                     /locus_tag="OQM34_004309"
     CDS             complement(25..297)
                     /locus_tag="OQM34_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454479.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           81 a           84 c           95 g           97 t
ORIGIN      
        1 cgtttcagcg ggcggggctg aaaatcacca ccacttcggg tcatccgcct tcatctccgc
       61 ttctgtttcg tataaatcaa aacgacggca ggtatggcag aacgtgacgt attcgtacgg
      121 tgaatccggg tacattttgt ggaaggtttc ccagaaacag accgtgcagg agggatgacc
      181 ggtgataaag tctgcaaata cggacttata agggtgatta ttggctctgg cgacggcttt
      241 cagaacctct ttcaccattc tggtgtggac tttctggtgc tccaggttgt gtgacatggg
      301 aactcattct ggatggttac tctgaaagct catattctgc cacaccccga tttgcag
//
LOCUS       DAJVPP010000382          356 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVPP010000382 DAJVPP010000000
VERSION     DAJVPP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.393"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>356)
                     /locus_tag="OQM34_004310"
     CDS             complement(<1..>356)
                     /locus_tag="OQM34_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1454480.1"
                     /translation="ESGAVDCDVFFDDRDQAVPYTATADDVAPTGQQIWQELQSGKWG
                     EIAPFTVTPEMLEAAREARRQEIEAWRTEQEAKPFTFEWNGRTWNADASSVARLSPVV
                     MLAKSVAAQTHMVWSDA"
BASE COUNT           64 a          112 c           89 g           91 t
ORIGIN      
        1 gcatcactcc acaccatatg tgtttgtgcc gcgacagatt ttgccagcat gaccaccggg
       61 gacaggcggg ccactgacga ggcgtcagcg ttccacgtgc ggccgttcca ttcaaacgtg
      121 aacggcttcg cctcctgttc tgtgcgccat gcttcaattt cctgacgtct ggcctctctg
      181 gccgcttcca gcatttctgg tgtcacagtg aatggggcta tctcacccca tttgccactt
      241 tgcagttcct gccagatttg ctgacccgtc ggtgcaacat catcagcggt ggctgtgtag
      301 ggaactgcct ggtccctgtc gtcaaaaaaa acgtcacagt ctactgcgcc actttc
//
LOCUS       DAJVPP010000383          349 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVPP010000383 DAJVPP010000000
VERSION     DAJVPP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.394"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..347
                     /locus_tag="OQM34_004311"
     CDS             <1..347
                     /locus_tag="OQM34_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1454481.1"
                     /translation="GGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQS
                     WLKENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVS
                     PFPGGKHGLAKV"
BASE COUNT          100 a           88 c           92 g           69 t
ORIGIN      
        1 tgggcggcaa cagcaaaagt tcggcgctgt tcatcagcct gctgaagcgg cttaaagcga
       61 cataccgtcg tgcgaaaacc atcacgctga tcgtggacaa ctacattatc cacaaaagcc
      121 gggaaacaca gagctggctg aaggagaacc cgaagttcag gggcatttat cagccggttt
      181 actcgccatg ggtgaatcat gttgaacggc tatggcaggc acttcacgac acaataacgc
      241 gtaatcatca gtgccgctca atgtggcaac tgttgaaaaa agttcgccat tttatggaaa
      301 ccgtcagccc attccccgga ggcaaacatg ggctggcaaa agtgtagcg
//
LOCUS       DAJVPP010000384          348 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJVPP010000384 DAJVPP010000000
VERSION     DAJVPP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.395"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
BASE COUNT          109 a           67 c           51 g          121 t
ORIGIN      
        1 gtttccatcg tattattcat ttcatcactg ttttagcatc acgtttcaat agtatccttt
       61 catagggcta cccaggatgc ggtcaccttc attgtcgctc taagcgacta gatcatcaga
      121 aatctccagt tagattgcat acttccttac acactttcgt gaaacttcag ggtaaggtga
      181 gaaagccgtc atcccttttt ctaattccag catctaaata aattccgaat tttgttatta
      241 gtatcgctac cgcaataaaa acagatagta aagagagtaa tatatacttt attgatatat
      301 taagaataat taattgttaa catatctaat ccaataagat gttatgtt
//
LOCUS       DAJVPP010000385          347 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJVPP010000385 DAJVPP010000000
VERSION     DAJVPP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.396"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            69..>347
                     /locus_tag="OQM34_004312"
     CDS             69..>347
                     /locus_tag="OQM34_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1454482.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLNTPGSRTVAELESEVMQLRKALNEARLERDILKKATAYFAQ"
BASE COUNT          111 a           76 c           84 g           76 t
ORIGIN      
        1 gagttgaggt agcctgagtt taacggacac tccttcctga aatagaatgg catcagaagg
       61 agccaataat gagcagaaaa acccaacgtt actctacgga gtttaaagcc gaagccgtca
      121 aaacggttcc tgaaaatcag ctttcgatca gtgaaggcgc ttcccgatta tctgttcctg
      181 aaggcacttt aggacaatgg gttaccgccg caagaaaagg gctcaataca ccaggctccc
      241 gcacagtggc tgagctggaa tctgaagtta tgcaactgcg taaggcttta aatgaagcac
      301 gacttgagcg agatatatta aaaaaagcaa cagcgtattt tgcacag
//
LOCUS       DAJVPP010000386          346 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVPP010000386 DAJVPP010000000
VERSION     DAJVPP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.397"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>346
                     /locus_tag="OQM34_004313"
     CDS             <1..>346
                     /locus_tag="OQM34_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCS1454483.1"
                     /translation="WIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSP
                     FSFVLPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQT
                     AVPVQASGGATTT"
BASE COUNT           79 a           96 c           96 g           75 t
ORIGIN      
        1 tctggatccg tcagaaagtc gcactgccgc ctgatgctgt gctgaccgtg acactttctg
       61 acgcgtcgtt agccgatgca ccgtcaaaag tgctggcgca gaaagcggtg cgtactgaag
      121 gtaaacagtc accattcagc tttgttctgc catttaaccc ggcagatgtt cagccgaacg
      181 cgcgtattct gttgagtgcg gcgattaccg tgaatgacaa actggtattt atcaccgata
      241 ccgttcagcc ggtgatcaac cagggcggaa ctaaagccga cctgacattg gtgccggtac
      301 agcaaaccgc cgtgccggtt caggccagcg gtggcgcaac gactac
//
LOCUS       DAJVPP010000387          346 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVPP010000387 DAJVPP010000000
VERSION     DAJVPP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.398"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>346
                     /locus_tag="OQM34_004314"
     CDS             <1..>346
                     /locus_tag="OQM34_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCS1454484.1"
                     /translation="AWAASGYSVVPRDLLIPPEQFALLSSIIVSSAGNQSLLTNLQTN
                     TISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPLQSVPVQYRGLY
                     QIVTYYGKLGAVE"
BASE COUNT           70 a           93 c           95 g           88 t
ORIGIN      
        1 tgcgtgggcc gcttctggtt actctgtggt tccgcgtgat ttgctgatcc cgcctgagca
       61 gtttgctctg ttgtccagca tcatcgtttc atctgcaggt aaccagtccc tgttgacgaa
      121 ccttcagacc aacaccatca gctatcacca gaacggtgtt ccgctgaata tccgcgcggt
      181 taaatggctg aaaggccgtg gtgtggggaa taaggatcgc atggttgcgt acaccaacga
      241 taaaaaatac gtccgctacc cgctggttcc gcttcagagc gtgccggtgc agtatcgcgg
      301 tctgtatcag atcgtcactt actacggcaa gctgggtgca gtcgag
//
LOCUS       DAJVPP010000388          342 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJVPP010000388 DAJVPP010000000
VERSION     DAJVPP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.399"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(1..111)
                     /locus_tag="OQM34_004315"
                     /pseudo
     CDS             complement(1..111)
                     /locus_tag="OQM34_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            106..342
                     /locus_tag="OQM34_004316"
                     /pseudo
     CDS             106..342
                     /locus_tag="OQM34_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           81 a          103 c           98 g           60 t
ORIGIN      
        1 acttccgtaa agaagggcat caagctcctg ctccgacata cctgcaggca aaggccatga
       61 aagccctgat ttccggaagc gagcgaacag tccacaaact gttgaacgtg gtgtcagcgg
      121 cggacaggga taagccctcg tcagcagttc cgtcagcact gcgacagcgt tctttacccg
      181 gtcaaaacag cgttgcggtg caccacgcct gacggatgaa ctgcgtgctc agggttacca
      241 ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag gggctgaggg caaaggcctc
      301 ccggaagttc agcccggtca gttaccgcgc acatggcctg cc
//
LOCUS       DAJVPP010000389          342 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJVPP010000389 DAJVPP010000000
VERSION     DAJVPP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.400"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..292
                     /gene="curA"
                     /locus_tag="OQM34_004317"
     CDS             <1..292
                     /gene="curA"
                     /locus_tag="OQM34_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1454485.1"
                     /translation="VSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDYGHRIH
                     EFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNFGKVVIRVAGDD"
BASE COUNT           99 a           72 c           93 g           78 t
ORIGIN      
        1 agtgagcagc tataacgcta cagagctacc acccggtccg gatcgtttac ccctgttgat
       61 ggcaacggtg ctgaaaaaac gtattcgctt gcaagggttt attatcgctc aggattatgg
      121 tcaccgcatc catgagtttc agaaggagat ggggcaatgg gtgaaagagg ataaaatcca
      181 ctaccgcgaa gatattactg atggtttaga gaatgcgcca cagacgttta tcggcctgct
      241 gaagggtaaa aacttcggca aagtggtgat ccgcgtcgcg ggtgatgatt aaagtttcaa
      301 cggcggcgta agccgccata aaaatccagg caaaaagctt at
//
LOCUS       DAJVPP010000390          342 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJVPP010000390 DAJVPP010000000
VERSION     DAJVPP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.401"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            37..>342
                     /locus_tag="OQM34_004318"
     CDS             37..>342
                     /locus_tag="OQM34_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1454486.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     "
BASE COUNT           87 a           92 c           81 g           82 t
ORIGIN      
        1 tggttatctg gttaacccat atacaaggga gacagaatga ccgaatccag cgattacgaa
       61 tccgtccagg tctttatcgg cgttgatgtc ggtaaagata cgcatcacgc tgttgccatt
      121 aatcgttcag gtaaacgcct gttcgataaa gcattaccca acgacgaaaa caaactcagg
      181 tcgctaatat ctgacctgaa acaacatggt cagatactgc tggttgttga tcagccagct
      241 accatcggtg cgttacctgt cgccgttgcc cgctcagaag gagtccttgt cggatacctc
      301 cctggactgg ccatgcgccg catagccgac ttacacgccg gt
//
LOCUS       DAJVPP010000391          341 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJVPP010000391 DAJVPP010000000
VERSION     DAJVPP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.402"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>341
                     /locus_tag="OQM34_004319"
     CDS             <1..>341
                     /locus_tag="OQM34_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1454487.1"
                     /translation="PKPIERGYASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSR
                     NTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVR
                     DDRNAGSSLPAA"
BASE COUNT           89 a           80 c          101 g           71 t
ORIGIN      
        1 cctaaaccga tcgaacgcgg ttatgccagt gcagggttac ttgcacggat cctggtcagc
       61 aaatatatgg aacatatccc tttatatcgc cagtcagaaa tatacgcgcg acagggcgtg
      121 gagctgagcc gtaataccat ggtgcgctgg gtatcagaaa tggcagacaa actccgtcct
      181 ctgtatatag cgctgaatga ctatgttctg gaggcaggaa aggtgcacgc agatgacact
      241 ccggtgaaag tactggcccc ggggaacgga aagacgaaaa cgggtcgtct gtgggtatac
      301 gtcagggatg atcgtaatgc gggttcatcc ctgccggcag c
//
LOCUS       DAJVPP010000392          340 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJVPP010000392 DAJVPP010000000
VERSION     DAJVPP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.403"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            106..>340
                     /locus_tag="OQM34_004320"
     CDS             106..>340
                     /locus_tag="OQM34_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1454488.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIP"
BASE COUNT           93 a           71 c          101 g           75 t
ORIGIN      
        1 ggggttgctt atcgacgacg agcagtttga tgaaaccaat attgtgcgcg gactgcctgt
       61 tcctggtggt cggttgggga taaaaatcac agagctggag tgtgcatgaa taaccagtat
      121 ttacagtttg ttcgtgagca gctcattatc gccaccgctg atttgagtgg ggcgacaaaa
      181 ggtcagcttg aagcctggca ggagaatgcc atgttcgata cagggcgtta caggcgtaaa
      241 aaaatccggt accgcgatga ggtgaccgga aaaatgataa cgcgggataa tccaccaatc
      301 ccgggaaaac aatcactggc gaagggggcg tcaattcctc
//
LOCUS       DAJVPP010000393          339 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJVPP010000393 DAJVPP010000000
VERSION     DAJVPP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.404"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
BASE COUNT           90 a           68 c           75 g          106 t
ORIGIN      
        1 aagaaaggtt aattattggt gttagctata taaaaagtaa caacagcaat gcatatgaat
       61 aattaaaggt tatgcaacgt gcaaagatta aatttctcag tttaatgaca ggttgctgta
      121 cttgcgtttg ttgctcttgc cggatgcggc gtaaacgcct tatccggcct acagttcatt
      181 gcaaatccat tcaatttcaa tgcacgatgt aggcctgata agcgtagcgc atcaggcaat
      241 tttaatgttt aacttccctg tttaatcagt tactgctggc gtagctggag atccagcggc
      301 gttttgctgg gttcgccgcc aatttctcgc gccagtttc
//
LOCUS       DAJVPP010000394          339 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJVPP010000394 DAJVPP010000000
VERSION     DAJVPP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.405"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(141..>339)
                     /locus_tag="OQM34_004321"
     CDS             complement(141..>339)
                     /locus_tag="OQM34_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454489.1"
                     /translation="STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLEL
                     ARANGWFVVRRDKLVERLMQL"
BASE COUNT           98 a           87 c           65 g           89 t
ORIGIN      
        1 ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca agtgtccatg
       61 tctgctattc gcctcgcgat acagctttgc gaagcttacc ggaattgaag cagtttatgt
      121 gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt atctctgcga
      181 acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga aataacaata
      241 tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata acagagaact
      301 cgcgtgtcgt aacttttctg gcatgaaact ggcgttgac
//
LOCUS       DAJVPP010000395          336 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJVPP010000395 DAJVPP010000000
VERSION     DAJVPP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.406"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(12..88)
                     /locus_tag="OQM34_004322"
     tRNA            complement(12..88)
                     /locus_tag="OQM34_004322"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Lys,seq:ttt)
     gene            complement(90..165)
                     /locus_tag="OQM34_004323"
     tRNA            complement(90..165)
                     /locus_tag="OQM34_004323"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Ile,seq:cat)
BASE COUNT           84 a           92 c           81 g           79 t
ORIGIN      
        1 caagtcggca ctggaccgcc accggggact cgaacctcgc acactcaact taaagggttg
       61 acgctctttc ctgatgagct agtggcggtt ggtggccctt gctggatttg aaccagcgac
      121 ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccgggcg caggataata
      181 acgttacgaa atcaatgttg caagcattca aaaatcaccc ttatctcctc caccagcgca
      241 ttcaccatgt ctatccgaga taagtggcac aaaaaacccg cttgtgggcg ggttttgttt
      301 gcttttgcca tcacgtacaa aatcggcaaa gtatca
//
LOCUS       DAJVPP010000396          335 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJVPP010000396 DAJVPP010000000
VERSION     DAJVPP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.407"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..>335)
                     /locus_tag="OQM34_004324"
     CDS             complement(<1..>335)
                     /locus_tag="OQM34_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1454490.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAF"
BASE COUNT           94 a           94 c           97 g           50 t
ORIGIN      
        1 gaacgctccc agacgtttca cgaacgccag gtacagtgcg atgagaataa tcggcaccag
       61 ggtgaatgcc gccgccatcg gcatattgcc aatcgccccc tgctgggaat aaaccatatt
      121 gccgataaaa tatcctggag gaccaaccag ctgcgggacg ataaaatcgc ccagtgtgag
      181 tgagaaggta aagatagagc cagcggcaat acccgggatt gccagcggca gcaccacata
      241 gcgaaaggtt tgtcgtggac gtgcgccgag atcagccgac gcctgcaaca atgacggcgg
      301 caaacgctca agcgccgcct gaacgggcag gatca
//
LOCUS       DAJVPP010000397          335 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJVPP010000397 DAJVPP010000000
VERSION     DAJVPP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.408"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>335
                     /locus_tag="OQM34_004325"
     CDS             <1..>335
                     /locus_tag="OQM34_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCS1454491.1"
                     /translation="ERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQ
                     RYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQKQGKAIALKTGAAAAKSEY
                     LVCIDGDAL"
BASE COUNT           96 a           80 c           83 g           76 t
ORIGIN      
        1 gtgaacgcca ctggccgtgg ggagaaaacg caccagctcc ccagttgaaa gataatccgt
       61 ctatctccat tatcattccc tgttttaatg aggagaaaaa cgttgaggaa accatacacg
      121 ccgctttagc acagcgttat gagaacattg aagttattgc cgtaaatgac ggttcaacag
      181 ataaaacccg tgccatcctg gatcgcatgg ctgcacaaat tccccatttg cgggtcattc
      241 atctggcgca aaaacagggg aaagccattg cgcttaaaac cggagctgcc gcggcgaaaa
      301 gtgaatatct ggtgtgcatt gatggcgatg cgtta
//
LOCUS       DAJVPP010000398          328 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJVPP010000398 DAJVPP010000000
VERSION     DAJVPP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.409"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            68..>328
                     /locus_tag="OQM34_004326"
     CDS             68..>328
                     /locus_tag="OQM34_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1454492.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEF"
BASE COUNT           74 a           69 c          102 g           83 t
ORIGIN      
        1 gaatgaattg tcagttttga tacgctagcg tagccttata gatctaaggt gtatcaggag
       61 ataacggatg aaaaaggtcg caattgtcgg gttagggtgg ttaggtatgc cgctggcgat
      121 gtcactttca gcgcgaggct ggcaagtcac cgggagtaaa accacacaag atggtgtcga
      181 agcggcccga atgagtggca ttgatagcta tctgcttcgc atggagcctg agttagtttg
      241 cgattctgac gatctggatg cccttatgga tgccgatgcg ctggtcatta cgcttccggc
      301 acgtcgtagc ggccccggcg atgagttc
//
LOCUS       DAJVPP010000399          328 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJVPP010000399 DAJVPP010000000
VERSION     DAJVPP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.410"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(134..>328)
                     /locus_tag="OQM34_004327"
     CDS             complement(134..>328)
                     /locus_tag="OQM34_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1454493.1"
                     /translation="QGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANFV
                     MAADRGSMIEGGIKGMKLVG"
BASE COUNT           81 a           99 c           66 g           82 t
ORIGIN      
        1 gtgcagaatc accaatccta atattactac taaatagctg cgcctaataa cgcgacaccc
       61 gccgtttact gtctggtggc ttaacctgca agggctggct gaaagtggga cactatgccg
      121 cgcagctatt tagtcaccca actagcttca ttccttttat cccaccttca atcatgcttc
      181 cacgatccgc tgccatcaca aaatttgccc accattgcat catcgggcga cgctgttcca
      241 ggtaatcgct gcggttataa gcacgacgaa cctcattctt atccacatgg gccaatgctg
      301 cctcaatggc atcaggcgga aagccttg
//
LOCUS       DAJVPP010000400          327 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJVPP010000400 DAJVPP010000000
VERSION     DAJVPP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.411"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            1..327
                     /locus_tag="OQM34_004328"
                     /pseudo
     CDS             1..327
                     /locus_tag="OQM34_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           82 a           79 c          101 g           65 t
ORIGIN      
        1 acgtattacc actgtcagca acagcgacat catccggata aacgcagtgc ccgtgcgcag
       61 cgcgatgact ggctgaagaa agagatacag cgcgtatacg atgaaaatca caaggtatac
      121 ggtgtaaagt ctggcgtcag ttgttacggg aaggtatcag agtggccaga tgcactgtgg
      181 cacgtctcat ggcggttatg ggacttgccg gtgttctccg gggtaaaaag gtccgtacga
      241 ccatcagccg gaaagccgtt gccgcaggcg accgcgtaaa ccgtcagttc gtggcagaac
      301 gacctgacca gctgtgggtg gctgatt
//
LOCUS       DAJVPP010000401          323 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJVPP010000401 DAJVPP010000000
VERSION     DAJVPP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.412"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
BASE COUNT          108 a           78 c           65 g           72 t
ORIGIN      
        1 attccagtcc atgcaaaatc tgatttccag ctcacacata ccgagcgctg tacaaaaaac
       61 cgccagccaa ggcagttaag agtgtgttcc cggggtttgc ttagaatatt ttgtaagttg
      121 tccgaagaga tatttacaac accagaatga tgaatcatca gtcccctgcc agaggttcac
      181 tacgtaacct gaccgacaat gcaccattct ggtgctgaaa aaagagcact ggaactgcaa
      241 caaaacaaca tcacagaaca ggagatggta aggagtcaga cattcccaca caatattgtg
      301 tcagtgcaaa taacaacctt cgt
//
LOCUS       DAJVPP010000402          323 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJVPP010000402 DAJVPP010000000
VERSION     DAJVPP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.413"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
BASE COUNT          125 a           45 c           66 g           87 t
ORIGIN      
        1 ttctgggaac tatattctca aaatatagta gaaacgaggt atgtttctgg tggaaaggac
       61 agtgggatta aaaagtaaga attgataaaa aaaacgccag caacacatag tgccggcgga
      121 gggaataccc catggagaaa atgtgatgcc tagagcatca tgaatatata attaaaaata
      181 gttagcgttg tcaatacttt cacaaaaata attttcgtag tatagaaaga tatttttatg
      241 catgacctac ctgaatttgc tccgggtaga ggttataaat aaaaattgaa tcacgacaaa
      301 cacaatattc atagtatggc gat
//
LOCUS       DAJVPP010000403          321 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJVPP010000403 DAJVPP010000000
VERSION     DAJVPP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.414"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..258
                     /locus_tag="OQM34_004329"
     CDS             <1..258
                     /locus_tag="OQM34_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1454494.1"
                     /translation="SGVHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVAS
                     ASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
BASE COUNT           81 a           78 c           74 g           88 t
ORIGIN      
        1 agcggtgtgc ataatcactt tggcgtcagc tggtttaacc ctgaagatct tgccactaag
       61 cgtaaagtga tgaaccgtgg actacggaaa attttgctcg cccgccacgc gttgttcgat
      121 gaagtggcct ctgccagcct cgcaccgatc tctgcatttg acgttctgat tagcgatcgt
      181 ccgttaccgg cagattatgt tacgcactgc cagaatggtt ctgtaaagat cattacacct
      241 gattcagaag acgaatgact tactgaaaaa acaccacaat cttgttaaac atcgtcgggt
      301 tggactgatt acgttgcact t
//
LOCUS       DAJVPP010000404          320 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJVPP010000404 DAJVPP010000000
VERSION     DAJVPP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.415"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>320
                     /locus_tag="OQM34_004330"
     CDS             <1..>320
                     /locus_tag="OQM34_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1454495.1"
                     /translation="IIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSDTADQ
                     IVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGT
                     PLTA"
BASE COUNT           81 a           73 c           85 g           81 t
ORIGIN      
        1 atattatagg cggtgcattc actctggcat ctctaatgct ggccgggcat gcactggcag
       61 aagatggtgt tgttaacttc gtcggtgaaa ttgtcgacac tacttgtgaa gttacctccg
      121 atacagccga tcaaattgtc ccactgggta aagtcagtaa aaatgcattt tcaggtgtag
      181 gtagtctggc gtcgccacag aagttcagta ttaaacttga aaattgcccg gcaacgtaca
      241 ctcaagcagc cgttcgtttt gatggtacag aagcgcctgg cggcgacggc gacctgaaag
      301 tgggtacgcc gcttacagca
//
LOCUS       DAJVPP010000405          319 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJVPP010000405 DAJVPP010000000
VERSION     DAJVPP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.416"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(31..89)
                     /locus_tag="OQM34_004331"
                     /pseudo
     CDS             complement(31..89)
                     /locus_tag="OQM34_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           70 a           95 c           72 g           82 t
ORIGIN      
        1 gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta cgagtaacaa
       61 ccaaccagta tgatgacgaa cttcatcata accctttcct tctataaggc cccttcctcg
      121 ggaggggctt tcccgtttca gccccttgct gactccccga ttgttgatga gcgccggacc
      181 ccgcgcaacg ctttccctga agcagttttg ccttccccga gcggtcacgc aaaacgctgc
      241 aacgtcctga gcatttgcac cttttagcgg aacgcagata taaaaaatcc ccggcttttt
      301 gggccaggga tatgtgtta
//
LOCUS       DAJVPP010000406          318 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJVPP010000406 DAJVPP010000000
VERSION     DAJVPP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.417"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>318
                     /locus_tag="OQM34_004332"
     CDS             <1..>318
                     /locus_tag="OQM34_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1454496.1"
                     /translation="EYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKA
                     ELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSKKR
                     RHF"
BASE COUNT          103 a           56 c           84 g           75 t
ORIGIN      
        1 gggagtatca gatcaccata acccactggg cgtgggaaga gtttaaggct cattctggca
       61 ctaggaaaat tgcagagaaa acacaggaac gcctgaaaaa attaatctgg ctggcggcgc
      121 aggcggtaaa agcagaactt tttggtgggg aaggttatga atatcaggag ctggcattac
      181 tggcgggagt aacaaccaaa aactggtcca aaacatttac tggtcactgg gttgtaatga
      241 aacacatttt tcaccgactg gatagtgagg ctttattgtt tgtaatgaga acacgttcaa
      301 aaaaaagacg gcattttc
//
LOCUS       DAJVPP010000407          313 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJVPP010000407 DAJVPP010000000
VERSION     DAJVPP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.418"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>313
                     /locus_tag="OQM34_004333"
     CDS             <1..>313
                     /locus_tag="OQM34_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="photosystem II reaction center protein Ycf12"
                     /protein_id="HCS1454497.1"
                     /translation="IDFSWFKKKQNHKIELFLTQPLVAGFLSLIMLSAMSWGLYQLLP
                     SLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYKSLTRYVLLSVCISTVV
                     NL"
BASE COUNT           76 a           64 c           70 g          103 t
ORIGIN      
        1 atcgattttt cgtggtttaa gaaaaagcag aaccacaaaa tagaattatt tcttacacaa
       61 cctctggtgg caggatttct gtcgttaatc atgttgagtg caatgagttg ggggctatac
      121 cagcttctac cctcgttata tgcgctgttc ctgatttcgt tacttgggcc gttggtcatc
      181 tttctgctgt ttcggatgat ccgcaccagt tatgtcaagc aggtcgctat ttcagtagcg
      241 aaaaaagtaa aatataaaag tctgactcgc tatgtgctgc tttcggtgtg catctcaacg
      301 gttgttaacc tgc
//
LOCUS       DAJVPP010000408          313 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJVPP010000408 DAJVPP010000000
VERSION     DAJVPP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.419"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
BASE COUNT          107 a           39 c           61 g          106 t
ORIGIN      
        1 tattgatatg aattttctat gtgttaaaaa gagtataaat gcctgacttt gctgcgccag
       61 gggccgagtc agaatacgag gtgatttatt aatagaacaa gttatatgta tatctaaaat
      121 taaattgttg aacataactc cttttgctat tggcgttatg ttcaacccag tcaaatgtta
      181 tttaatataa tgcgtgactg ttgaataagt taatttaaaa attttgagca aatttttcat
      241 ctggttaggt attaattaaa caaataaagt tactgtgcag ccataaaaat gaagccggga
      301 tagcggtgga gac
//
LOCUS       DAJVPP010000409          310 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJVPP010000409 DAJVPP010000000
VERSION     DAJVPP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.420"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(50..>310)
                     /gene="osmC"
                     /locus_tag="OQM34_004334"
     CDS             complement(50..>310)
                     /gene="osmC"
                     /locus_tag="OQM34_004334"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1454498.1"
                     /translation="ACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALK
                     SEVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT           76 a           88 c           68 g           78 t
ORIGIN      
        1 taacaacgca tcaggcattc acgcgcctga cgcgtcatcc ggcaatgctt tacgatttca
       61 actggtaatc cagcgtaatt tccgctttca gtacctgaga gaccgggcat cctgcttttg
      121 ctttctggat gatgccgtca aaggtagagg catcaatacc cggcaccgca acttcactct
      181 tcagtgcgat tttcgtaatc gcaaaaccgg catccacttt atccagcgac acatcggcgg
      241 tggtatcaat tgatgttggc gtgaatcccg cttcccccag cattaatgaa agcgccattg
      301 agaaacatgc
//
LOCUS       DAJVPP010000410          310 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJVPP010000410 DAJVPP010000000
VERSION     DAJVPP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.421"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>310
                     /locus_tag="OQM34_004335"
     CDS             <1..>310
                     /locus_tag="OQM34_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1454499.1"
                     /translation="ITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSK
                     INQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNR
                     I"
BASE COUNT           96 a           58 c           58 g           98 t
ORIGIN      
        1 tattaccgcg aacgaaaata aggttggaac gacatttaac accactgtaa ctaatggatc
       61 ctcatatctt ggacgctgtc attgtgatac tggtaacgta ggagaattcc cctatatcta
      121 ttatacgtca aaaataaacc aggcattaac ttatgctggg gttcactcta atattaatta
      181 ttatgatctt aatccgaacc ttgatgttgg gattgctata gatattcttg gggttgggta
      241 tgtgaatgca ccttttgaat atcatgctaa caacccgtct ggtaatacaa agtacaattg
      301 caaccgcatt
//
LOCUS       DAJVPP010000411          309 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJVPP010000411 DAJVPP010000000
VERSION     DAJVPP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.422"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..157
                     /locus_tag="OQM34_004336"
     CDS             <1..157
                     /locus_tag="OQM34_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1454500.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
BASE COUNT           85 a           73 c           78 g           73 t
ORIGIN      
        1 aattgaacaa aaaatgttac agcacacgcc gatcagacgt ctgggccaac cgcaagatat
       61 tgctaacgcg gcgctgttcc tttgctcgcc tgcagccagc tgggtaagcg gacaaattct
      121 caccgtctcc ggtggtgggg tacaggagct caattaatac actaacgaac cggtaaacgg
      181 tcgtatgtgt tgtttaccgg gatagcgtgt gcaattcatc aacgtctctg ctaaacaact
      241 ggcaggcaaa tcacggctat tggttaacca attgctgggt gaaaagtata cgaatagagt
      301 gtaccttcg
//
LOCUS       DAJVPP010000412          308 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJVPP010000412 DAJVPP010000000
VERSION     DAJVPP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.423"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>308
                     /locus_tag="OQM34_004337"
     CDS             <1..>308
                     /locus_tag="OQM34_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCS1454501.1"
                     /translation="RTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPLGQALNK
                     MHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIAGNIEEL
                     "
BASE COUNT          100 a           57 c           78 g           73 t
ORIGIN      
        1 aggacggggt tgcgagagat caaaaagttg agtggcgtaa cggaagcgct gaattataac
       61 gatagccgag tgccggttga ggttaacgca ttaccgagag aactaaaacc tcttgggcag
      121 gcgttgaata aaatgcatca tgctttagtc aaagattttg agcgccttag tcaatttgct
      181 gacgatctcg ctcatgaact tagaacgcca attaatgcat tactgggtca gaatcaggtt
      241 acgctcagtc aaatcagaag tatcgctgaa tatcaaaaaa caattgccgg aaacattgaa
      301 gagctgga
//
LOCUS       DAJVPP010000413          308 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJVPP010000413 DAJVPP010000000
VERSION     DAJVPP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.424"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>308
                     /locus_tag="OQM34_004338"
     CDS             <1..>308
                     /locus_tag="OQM34_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCS1454502.1"
                     /translation="RYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGK
                     KLPERETLEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELY
                     "
BASE COUNT           74 a           66 c           90 g           78 t
ORIGIN      
        1 gcgttatttc tcgctcgcga ctcgaatcgc cgctgaaatg ggggcgcaaa ttatcaaaac
       61 ctattatgtc gaaaaaggtt ttgaacggat tgttgccgga tgtccggtac ccattgttat
      121 tgctggcggt aaaaaattac cggagcgcga gacgctggaa atgtgctggc aggctatcga
      181 tcagggcgct tctggtgtgg atatggggcg taatattttc cagtctgacc atccggtggc
      241 gatgatgaaa gccgtacagg cggtggttca ccataacgaa acggctgatc gggcatatga
      301 actctatc
//
LOCUS       DAJVPP010000414          306 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJVPP010000414 DAJVPP010000000
VERSION     DAJVPP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.425"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..>306
                     /locus_tag="OQM34_004339"
     CDS             <1..>306
                     /locus_tag="OQM34_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCS1454503.1"
                     /translation="EITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHPVIT
                     GHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMIIPE"
BASE COUNT           89 a           60 c           77 g           80 t
ORIGIN      
        1 cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc tgtttatcga
       61 cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat ttagaaaatc
      121 atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt aaaaatctca
      181 ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg ttaccaagcg
      241 gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat atgattattc
      301 ccgaaa
//
LOCUS       DAJVPP010000415          300 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJVPP010000415 DAJVPP010000000
VERSION     DAJVPP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.426"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..218
                     /gene="folK"
                     /locus_tag="OQM34_004340"
     CDS             <1..218
                     /gene="folK"
                     /locus_tag="OQM34_004340"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1454504.1"
                     /translation="RWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEI
                     APELAFPDGETLREVLHTRAFDKLSKW"
BASE COUNT           88 a           55 c           63 g           94 t
ORIGIN      
        1 aacgctgggg accacgcacg ctggatctcg atatcatgct gtttggtaat gaagtgataa
       61 ataccgaacg cctgaccgtt ccgcactacg atatgaagaa tcgtggattt atgctgtggc
      121 cgctgtttga gattgcaccg gagctggcgt ttcctgatgg ggagacgttg cgtgaagtct
      181 tacatacaag agcatttgac aaattaagca aatggtaatt tatttatatt acgaatataa
      241 atattttctg aaaatcatac atcctctctt tccttaataa aaattttagt ttaaacattt
//
LOCUS       DAJVPP010000416          299 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJVPP010000416 DAJVPP010000000
VERSION     DAJVPP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.denovo.427"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..84)
                     /locus_tag="OQM34_004341"
     CDS             complement(<1..84)
                     /locus_tag="OQM34_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCS1454505.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTA"
     gene            complement(219..>299)
                     /locus_tag="OQM34_004342"
     CDS             complement(219..>299)
                     /locus_tag="OQM34_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1454506.1"
                     /translation="SVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT           97 a           64 c           43 g           95 t
ORIGIN      
        1 tgcagtaaca ccacgttcat ccttcgcgaa acgcgcaaag cactgtttag ttttaccata
       61 gtacttagta ataaaagtat tcatgttttc ctcgtatatt attatttaaa ttcaaaccac
      121 cagcttcatc tgcgtgtcac cggtatccca tatttttggg atgtggctat aattatccat
      181 tcccgctatt tagtcttcaa aaaaaattac atttaatatc atttatcttg attaagaaat
      241 agtaagacta aaaaaacaag cgtcgtcagc cagcacactg ctaacacggt aagaacgct
//
LOCUS       DAJVPP010000417         5250 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.180, whole genome shotgun sequence.
ACCESSION   DAJVPP010000417 DAJVPP010000000
VERSION     DAJVPP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.180"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            70..285
                     /locus_tag="OQM34_004343"
     CDS             70..285
                     /locus_tag="OQM34_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1454507.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="OQM34_004344"
     CDS             278..664
                     /locus_tag="OQM34_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCS1454508.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="OQM34_004345"
     CDS             672..1358
                     /locus_tag="OQM34_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1454509.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="OQM34_004346"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="OQM34_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1454510.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="OQM34_004347"
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="OQM34_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1454511.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="OQM34_004348"
     CDS             complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="OQM34_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCS1454512.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4085..4456
                     /locus_tag="OQM34_004349"
     CDS             4085..4456
                     /locus_tag="OQM34_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1454513.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4466..>5250)
                     /locus_tag="OQM34_004350"
     CDS             complement(4466..>5250)
                     /locus_tag="OQM34_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCS1454514.1"
                     /translation="LADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWLSRVRGK
                     VQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSRSKGRKNQRS
                     TRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEETFRDLKSPA
                     YGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNVLS
                     TVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1459 a          966 c         1127 g         1698 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     3841 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     3901 gtgattgata ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt
     3961 taaaataaca taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta
     4021 agaacattta ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa
     4081 taacatccaa ttaagatatg atgataagca gtttcataca acggttatca aagatgttct
     4141 attatggatt gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc
     4201 gggttatacc aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc
     4261 tagctatatt cgtgctcgtc gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa
     4321 aactatcctt gagatcgcat taaaatatca atttgattcc caacaatctt ttacacgtcg
     4381 atttaagtac atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt
     4441 ggaggcaatg cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata
     4501 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag
     4561 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg
     4621 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac
     4681 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg
     4741 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt
     4801 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag
     4861 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg
     4921 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat
     4981 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct
     5041 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta
     5101 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa
     5161 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac
     5221 tcggtagaat gctcgcaagg tcggctagaa
//
LOCUS       DAJVPP010000418         4849 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.186, whole genome shotgun sequence.
ACCESSION   DAJVPP010000418 DAJVPP010000000
VERSION     DAJVPP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.186"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            607..1422
                     /gene="sul2"
                     /locus_tag="OQM34_004351"
     CDS             607..1422
                     /gene="sul2"
                     /locus_tag="OQM34_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1454515.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     PTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1483..2286
                     /locus_tag="OQM34_004352"
                     /note="aph(3'')-Ib"
     CDS             1483..2286
                     /locus_tag="OQM34_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1454516.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2286..3122
                     /locus_tag="OQM34_004353"
     CDS             2286..3122
                     /locus_tag="OQM34_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1454517.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3229..3633)
                     /locus_tag="OQM34_004354"
                     /pseudo
     CDS             complement(3229..3633)
                     /locus_tag="OQM34_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3677..4066)
                     /locus_tag="OQM34_004355"
     CDS             complement(3677..4066)
                     /locus_tag="OQM34_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454518.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4284..4724
                     /locus_tag="OQM34_004356"
     CDS             4284..4724
                     /locus_tag="OQM34_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1454519.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1144 a         1318 c         1267 g         1120 t
ORIGIN      
        1 cccgttcagt ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc caacccggaa
       61 agacacgaca aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga
      121 ttaagaggtc ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat
      181 ggcggtctcg tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa
      241 caaaccgcct ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa
      301 aggatctcaa gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag
      361 tcactgttga ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca
      421 gcaccaaaga agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc
      481 caaaatgcta cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg
      541 gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa
      601 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
      661 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
      721 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
      781 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
      841 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
      901 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
      961 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     1021 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     1081 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     1141 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     1201 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     1261 cccacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     1321 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     1381 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     1441 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     1501 ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt
     1561 gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt
     1621 gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc
     1681 gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg
     1741 ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg
     1801 cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga gcgcaggctg
     1861 tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc
     1921 ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag
     1981 ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc
     2041 atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg
     2101 cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag
     2161 aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg
     2221 atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg
     2281 ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca
     2341 ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa
     2401 tcgtcaaggg attgaaacct atagaagaca ttgctgatga actgcgcggg gccgactatc
     2461 tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt
     2521 tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc
     2581 aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc
     2641 tgccttctgc ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg
     2701 atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa
     2761 tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca
     2821 tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct
     2881 ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta
     2941 gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc
     3001 tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag
     3061 aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact
     3121 agatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc
     3181 tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgaccccaa acaggaaacg
     3241 cagctgaaac gggaagctca acacccactg acgcatgggt tgttcaggca gtacttcatc
     3301 aaccagcaag gcggcacttt cggccatccg ccgcgcccca cagctcgggc agaaaccgcg
     3361 acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag
     3421 aaagccatgc tccagccgcc cgcattggag aaattcttca aattcccgtt gcacatagcc
     3481 cggcaattcc tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac
     3541 gatctgatag agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc
     3601 ccggctggcc gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt
     3661 acatacagtc tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg
     3721 ctagacattg ccagccagtg cccgtcactc ccactcttac attgttcgca gtatctaggt
     3781 acatgttatt gcgtgcacct tcatgatgta taatgttatt aatgacagca gaaagatcga
     3841 cagaattaat attcttcgta cctgggacag ttatcgggtg tcggccagtg atgagaccaa
     3901 aggatgcagt ataagatgaa ccactactat tatcatggcc accattacca ccaccgttaa
     3961 aactacctcc cgaacttcca gagccgctat gaccatcatt gtaggggtca tttggtccca
     4021 tgtgaccaaa accaccgaca gtaccgctat ttaactctgt actcataaaa ttatcctctt
     4081 tgacttttaa aaacagcaaa aactgtattt ataacaatgt gtttatatac agtattcgaa
     4141 atactacgcc cgtcaaaacc acaccgtcaa caggtttttt gtgtattttt cacgcctcac
     4201 gggcagcctc agaccttttg tgttaaaaca gtaatatgtt ctgctgttac aatacggtac
     4261 acacaggaga cactgtcact tttatggcat ggcacacagt tcaagagaca ctgaaattaa
     4321 caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa aggccttgtc agctatcgca
     4381 caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact gattcgggcc tatggcgagc
     4441 tgcgcacttc tgagacacca aaaaggcaca aggagggaca ctccaaatct gtcgttagcc
     4501 aggcggaaaa tgctcagcta gacgtcattc agcagaaact ggacgctctg cagcagactg
     4561 ttaccctaat gcttgaagac aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg
     4621 agagggaaca actacaggcc gaaattagcc gtctggctga cgcgctggca caggaaaaaa
     4681 agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga gtaacagtgc aggtgactct
     4741 gatttaccat aacatctgtt atcagcaccg cctgagaggc cctcagggcg tttctgacgg
     4801 gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgc
//
LOCUS       DAJVPP010000419         4849 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.187, whole genome shotgun sequence.
ACCESSION   DAJVPP010000419 DAJVPP010000000
VERSION     DAJVPP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.187"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            607..1422
                     /gene="sul2"
                     /locus_tag="OQM34_004357"
     CDS             607..1422
                     /gene="sul2"
                     /locus_tag="OQM34_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1454520.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1483..2286
                     /locus_tag="OQM34_004358"
                     /note="aph(3'')-Ib"
     CDS             1483..2286
                     /locus_tag="OQM34_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1454521.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2286..3122
                     /locus_tag="OQM34_004359"
     CDS             2286..3122
                     /locus_tag="OQM34_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1454522.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3229..3633)
                     /locus_tag="OQM34_004360"
                     /pseudo
     CDS             complement(3229..3633)
                     /locus_tag="OQM34_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3677..4066)
                     /locus_tag="OQM34_004361"
     CDS             complement(3677..4066)
                     /locus_tag="OQM34_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1454523.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4284..4724
                     /locus_tag="OQM34_004362"
     CDS             4284..4724
                     /locus_tag="OQM34_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1454524.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1144 a         1317 c         1267 g         1121 t
ORIGIN      
        1 cccgttcagt ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc caacccggaa
       61 agacacgaca aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga
      121 ttaagaggtc ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat
      181 ggcggtctcg tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa
      241 caaaccgcct ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa
      301 aggatctcaa gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag
      361 tcactgttga ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca
      421 gcaccaaaga agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc
      481 caaaatgcta cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg
      541 gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa
      601 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
      661 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
      721 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
      781 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
      841 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
      901 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
      961 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     1021 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     1081 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     1141 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     1201 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     1261 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     1321 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     1381 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     1441 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     1501 ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt
     1561 gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt
     1621 gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc
     1681 gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg
     1741 ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg
     1801 cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga gcgcaggctg
     1861 tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc
     1921 ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag
     1981 ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc
     2041 atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg
     2101 cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag
     2161 aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg
     2221 atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg
     2281 ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca
     2341 ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa
     2401 tcgtcaaggg attgaaacct atagaagaca ttgctgatga actgcgcggg gccgactatc
     2461 tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt
     2521 tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc
     2581 aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc
     2641 tgccttctgc ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg
     2701 atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa
     2761 tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca
     2821 tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct
     2881 ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta
     2941 gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc
     3001 tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag
     3061 aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact
     3121 agatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc
     3181 tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgaccccaa acaggaaacg
     3241 cagctgaaac gggaagctca acacccactg acgcatgggt tgttcaggca gtacttcatc
     3301 aaccagcaag gcggcacttt cggccatccg ccgcgcccca cagctcgggc agaaaccgcg
     3361 acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag
     3421 aaagccatgc tccagccgcc cgcattggag aaattcttca aattcccgtt gcacatagcc
     3481 cggcaattcc tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac
     3541 gatctgatag agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc
     3601 ccggctggcc gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt
     3661 acatacagtc tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg
     3721 ctagacattg ccagccagtg cccgtcactc ccactcttac attgttcgca gtatctaggt
     3781 acatgttatt gcgtgcacct tcatgatgta taatgttatt aatgacagca gaaagatcga
     3841 cagaattaat attcttcgta cctgggacag ttatcgggtg tcggccagtg atgagaccaa
     3901 aggatgcagt ataagatgaa ccactactat tatcatggcc accattacca ccaccgttaa
     3961 aactacctcc cgaacttcca gagccgctat gaccatcatt gtaggggtca tttggtccca
     4021 tgtgaccaaa accaccgaca gtaccgctat ttaactctgt actcataaaa ttatcctctt
     4081 tgacttttaa aaacagcaaa aactgtattt ataacaatgt gtttatatac agtattcgaa
     4141 atactacgcc cgtcaaaacc acaccgtcaa caggtttttt gtgtattttt cacgcctcac
     4201 gggcagcctc agaccttttg tgttaaaaca gtaatatgtt ctgctgttac aatacggtac
     4261 acacaggaga cactgtcact tttatggcat ggcacacagt tcaagagaca ctgaaattaa
     4321 caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa aggccttgtc agctatcgca
     4381 caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact gattcgggcc tatggcgagc
     4441 tgcgcacttc tgagacacca aaaaggcaca aggagggaca ctccaaatct gtcgttagcc
     4501 aggcggaaaa tgctcagcta gacgtcattc agcagaaact ggacgctctg cagcagactg
     4561 ttaccctaat gcttgaagac aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg
     4621 agagggaaca actacaggcc gaaattagcc gtctggctga cgcgctggca caggaaaaaa
     4681 agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga gtaacagtgc aggtgactct
     4741 gatttaccat aacatctgtt atcagcaccg cctgagaggc cctcagggcg tttctgacgg
     4801 gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgc
//
LOCUS       DAJVPP010000420         3909 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.201, whole genome shotgun sequence.
ACCESSION   DAJVPP010000420 DAJVPP010000000
VERSION     DAJVPP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.201"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..322
                     /gene="frdB"
                     /locus_tag="OQM34_004363"
     CDS             <1..322
                     /gene="frdB"
                     /locus_tag="OQM34_004363"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1454525.1"
                     /translation="QMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNE
                     DSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIAT
                     LKPR"
     gene            333..728
                     /gene="frdC"
                     /locus_tag="OQM34_004364"
     CDS             333..728
                     /gene="frdC"
                     /locus_tag="OQM34_004364"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1454526.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            739..1098
                     /gene="frdD"
                     /locus_tag="OQM34_004365"
     CDS             739..1098
                     /gene="frdD"
                     /locus_tag="OQM34_004365"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1454527.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1161..2294
                     /locus_tag="OQM34_004366"
     CDS             1161..2294
                     /locus_tag="OQM34_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1454528.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            2383..2916
                     /gene="blc"
                     /locus_tag="OQM34_004367"
     CDS             2383..2916
                     /gene="blc"
                     /locus_tag="OQM34_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1454529.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2913..3230)
                     /gene="sugE"
                     /locus_tag="OQM34_004368"
     CDS             complement(2913..3230)
                     /gene="sugE"
                     /locus_tag="OQM34_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1454530.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3406..3552)
                     /gene="ecnB"
                     /locus_tag="OQM34_004369"
     CDS             complement(3406..3552)
                     /gene="ecnB"
                     /locus_tag="OQM34_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1454531.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3663..3788)
                     /gene="ecnA"
                     /locus_tag="OQM34_004370"
     CDS             complement(3663..3788)
                     /gene="ecnA"
                     /locus_tag="OQM34_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1454532.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(3840..>3909)
                     /locus_tag="OQM34_004371"
     CDS             complement(3840..>3909)
                     /locus_tag="OQM34_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1454533.1"
                     /translation="FVQIGEVIKVDTRSGEYVSRVK"
BASE COUNT          973 a          998 c         1002 g          936 t
ORIGIN      
        1 gcagatggca aagtatcacc agttctccgg ttgcatcaac tgtggtctgt gctacgccgc
       61 gtgcccgcag tttggcctga acccagagtt catcggtccg gctgccatta cgctggcgca
      121 tcgttataac gaagatagcc gcgaccacgg taagaaggag cgtatggcgc agttgaacag
      181 ccagaacggt gtatggagct gtactttcgt gggctattgc tccgaagtct gcccgaaaca
      241 cgtcgatccg gctgcggcca ttcagcaggg caaagtagaa agttcgaaag actttcttat
      301 cgcgaccctg aaaccacgct aaggagtgca acatgacgac taaacgtaaa ccgtatgtac
      361 ggccaatgac gtccacctgg tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg
      421 aaggcacggc ggttccggct gtgtggttca gcattgaact gattttcggg ctgtttgccc
      481 tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt tttacaaaac ccggttatcg
      541 tgatcattaa cctgatcact ctgacggcag ctctgctgca caccaaaacc tggtttgaac
      601 tggcaccgaa agcggccaat atcattgtaa aagacgaaaa aatgggacca gagccaatta
      661 tcaaaagtct ctgggcggta actgtggttg ccaccatcgt aatcctgttt gttgccctgt
      721 actggtaagg agcctgagat gattaatcca aatccaaagc gttctgacga accggtattc
      781 tggggcctct tcggggccgg tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg
      841 ctggtgggta ttctgctgcc actggggctg tttccgggtg atgcgctgag ctacgagcgc
      901 gttctggcgt tcgcgcagag cttcattggt cgcgtattcc tgttcctgat gatcgttctg
      961 ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc acgatctgaa aatccacgtg
     1021 cctgcgggca aatgggtttt ctacggtctg gctgctatcc tgacagttgt cacgctgatt
     1081 ggtgtcgtta caatctaacg catcgccaat gtaaatccgg cccgcctatg gcgggccgtt
     1141 ttgtatggaa accagacctt atgttcaaaa cgacgctctg cgccttatta attaccgcct
     1201 cttactccac atttgctgcc cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc
     1261 cgcttataga gcaacaaaag atccccggta tggcggtggc ggtaatttat cagggtaaac
     1321 cttattactt tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa
     1381 cgttgtttga gttaggttcg gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta
     1441 ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa atactggcct gaacttaccg
     1501 ctaaacagtg gaatgggatc acactattac atcttgcgac ctacaccgct ggcggcctgc
     1561 cattgcaggt gccggatgaa gtgaaatcct caagcgactt gctgcgcttc tatcaaaact
     1621 ggcagcctgc atgggctcca ggaacacaac gtctgtatgc caactccagt atcggtttgt
     1681 tcggcgcact ggctgtgaag ccgtctggtt tgagttttga gcaggcgatg caaactcgtg
     1741 tcttccagcc actcaaactc aaccatacgt ggattaatgt acctccccca gaagaaaaga
     1801 attacgcctg gggatatcgc gaaggtaagg cagtgcatgt ttcgccaggg gcgttagatg
     1861 ctgaagctta tggtgtgaag tcgaccattg aagatatggc ccgctgggtg caaagcaatt
     1921 taccccccct tgatatcaat gagaaaacgc ttcaacaagg gatacaactg gcacaatctc
     1981 gctactggca aaccggcgat atgtatcagg gcctgggctg ggaaatgctg gactggccgg
     2041 tcaatcctga cagcatcatt aacggcagtg acaataaaat tgcactggca gcacgcccgg
     2101 taaaagcgat taagccccca actcctgcag tacgcgcatc atgggtacat aaaacggggg
     2161 cgaccggcgg atttggtagc tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga
     2221 tgctggctaa caaaaactat cccaatccag cgagagtcgc cgccgcctgt cagattctca
     2281 acgctctaca gtaaaatttc atcgggtccg aattttcgga ccttttctgc gcttttcctt
     2341 gctgtcatct acacttagaa aaaaaccagt aaggaaacat ttatgcgcct gctccctctc
     2401 gttgccgcag cgacagctgc atttctggtc gttgcctgta gttttcctac gccgccgcgt
     2461 ggcgtgaccg tagtgaataa tttcgacgcc aaacgttatc tcggtacctg gtatgagatt
     2521 gcccgttttg atcaccgttt tgaacgtgga ctggaaaaag tcaccgcaac atacagcctg
     2581 cgtgatgacg gcggcctgaa tgtcattaat aaaggctata accctgacag aggaatgtgg
     2641 cagcagagtg aagggaaagc gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg
     2701 tcattctttg gtcctttcta tggcggttat aacgttattg cactcgatcg ggaataccgc
     2761 catgcgctgg tttgcggccc ggaccgcgac tacctgtgga tactctcccg cacgccaacc
     2821 atttctgacg aagtgaaaca ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc
     2881 agtaagttta tttgggtaca gcagcccggt agttagtgag tgctgagttt cagaccaata
     2941 atccccaata cgattaacgc cagactcgcc agacgcatcg ggttagcgga ctcaccgagc
     3001 agcacaatgc cggtgatggc cgcgccgact gcgccaatac ccgtccacac ggcataagcc
     3061 gtccctaccg gtaacgattt catcgcccag gcaagtagcg ccatactgac tatcatcgcc
     3121 gtcacggtaa taacactcgg cgtcaaacgg ctaaagccgt gggtatattt caggccaacg
     3181 gcccatacca cttccagcag accagcaata actaagataa tccaggacat atcaggctcc
     3241 ggaaatttgg ggccgtcccc tgtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat
     3301 ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt tatgacgatc
     3361 gcagaaaaaa caaacggcac gacactgctg tcgtaccgtt attgcttatt gctgcgcttt
     3421 cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt
     3481 ggtgttgcag gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt
     3541 cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt
     3601 aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga agagaagaaa
     3661 aattagctgg cagcgcgaga gatggagtta ccgagatgtt tgatgtcttc gccgaaaccg
     3721 caagcggtgt tacagcccgt gagcagcgtg ctggcaagca aaacaagaac gataaggcgt
     3781 ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat
     3841 tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatt
     3901 tgtacgaac
//
LOCUS       DAJVPP010000421         3493 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.210, whole genome shotgun sequence.
ACCESSION   DAJVPP010000421 DAJVPP010000000
VERSION     DAJVPP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.210"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            complement(<1..397)
                     /locus_tag="OQM34_004372"
     CDS             complement(<1..397)
                     /locus_tag="OQM34_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCS1454534.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENV"
     gene            659..1117
                     /gene="gpt"
                     /locus_tag="OQM34_004373"
     CDS             659..1117
                     /gene="gpt"
                     /locus_tag="OQM34_004373"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1454535.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1209..2453
                     /gene="frsA"
                     /locus_tag="OQM34_004374"
     CDS             1209..2453
                     /gene="frsA"
                     /locus_tag="OQM34_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1454536.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2511..2912
                     /gene="crl"
                     /locus_tag="OQM34_004375"
     CDS             2511..2912
                     /gene="crl"
                     /locus_tag="OQM34_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1454537.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(2951..>3493)
                     /locus_tag="OQM34_004376"
     CDS             complement(2951..>3493)
                     /locus_tag="OQM34_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1454538.1"
                     /translation="YQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTN
                     EQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQ
                     YQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDS
                     DNKLNINNDDTVAVGMTYQF"
BASE COUNT          853 a          845 c          890 g          905 t
ORIGIN      
        1 ccacgttttc gtcagccaga accgccagtg cagaggccat accaatgccg ttatccgcac
       61 ccagcgtggt gccgcgcgct ttaacccatt cgccatcaat ataaggctgg ataggatctt
      121 tcgtgaagtc atgcacggtg tcgttatttt tctgcggcac catatcgagg tgggcctgta
      181 agacgaccgg tttacgattt tccatacctg cggtagcagg tttacgaatc aggatattac
      241 ctacctgatc gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca atgtattcag
      301 cgagttgctc ttcatgatag gacgggtgag gaatagaaca gattttggca aaaatatccc
      361 acagcggctg tggagataat tgagacagtt cagacacgtt aagtctcctt gtcgatcacc
      421 cgcaaaacag tattgcaggt cacagggtta gcagaaaatg ttgtcaacac aagacaggct
      481 tgcgagatat gtattgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
      541 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
      601 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
      661 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
      721 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
      781 accgggggcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
      841 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
      901 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgctg ttgcgattcg
      961 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     1021 ggttgatgac tatgttgttg atatcccgca agatacctgg atcgaacagc cgtgggatat
     1081 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcattgcc
     1141 gggcgttgtt ctttttaact tcaggcaggt tacaatagtt tccagtaagt attctggagg
     1201 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     1261 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     1321 ctcttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     1381 ggcgcggtat tgacccacgt gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     1441 ctgaacgtac cgacgatgat ttatacgata ccgtgattgg ctaccgtggt ggcaactgga
     1501 tttatgagtg ggccacccag gcgatggtgt ggcaacaaaa agcctgtacg gaagaagatc
     1561 cgcaactcag tggtcgacac tggctgcatg cggctacgtt gtacaacatt gccgcctatc
     1621 ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttatcaaac cgcgcctatg
     1681 aagaggccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg
     1741 gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat
     1801 taatgtgtgg tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt
     1861 ttgcgccgcg cggcattgcg atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa
     1921 aatggaagct cacccaggac tccagcctgt tgcatcagca cgtcttaaag gcgctgccta
     1981 acgtaccgtg ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg
     2041 ttgccgtgcg tctggcatac cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc
     2101 cggtagttca taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc
     2161 ttgacgttct ggcgagtcgt ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg
     2221 agctgaatcg ctattcatta aaagtgcaag gattgctggg gcgtcgctgc ccaacgccaa
     2281 tgttatcagg ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca
     2341 cctcatcatc tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt
     2401 ttgacaaagg tcttcaggaa atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg
     2461 ctaaattttg ctaatttggt aaaacagttg catcacaaca ggagatagca atgacgttac
     2521 cgagtggaca cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc
     2581 gtgaaggtaa gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg
     2641 tgaaaccggc accggaagtg cgtgaattct ggggctggtg gatggagctg gaagcgcagg
     2701 aatcccgttt tacctacagc taccagtttg gtctgttcga taaagcaggc gactggaaga
     2761 gtgttccggt aaaagatact gaagtggttg aacgactgga gcacaccctg cgtgagtttc
     2821 acgagaagct gcgtgaactg ctgacgacgc tgaatctgaa gctggaaccg gcgaatgatt
     2881 ttcgtgatga accggtgaag ttaacggcgt gagtgaaatg tgccggatgc atcacatccg
     2941 gcaatattca ttaaaactga tacgtcatcc caaccgcgac agtatcatca ttattaatat
     3001 tcagcttatt atcgctatcc agttggttga ttttataatc aacaaacgct gacatatttt
     3061 tgttgaaata atacgtagca ccgacgtcga tataattgac cagatcttca tcaccgatgc
     3121 cctcaatatc tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa
     3181 actggtattg agcgaccgct tcaaagttct gtgtcttatt ggcaaagccg ccagttattg
     3241 gcgtcatttt gcgtgtttca gaatagaaag ttgccagata aatattattg gcatcgtatt
     3301 tcagacctgt tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct
     3361 cgttggtgcg atctgagttg gtataggccc cactaatggc gaaatcgctg ccgccaaagt
     3421 catatgtcaa tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcgt
     3481 ttttcccttg ata
//
LOCUS       DAJVPP010000422         3271 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.211, whole genome shotgun sequence.
ACCESSION   DAJVPP010000422 DAJVPP010000000
VERSION     DAJVPP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.211"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            335..574
                     /locus_tag="OQM34_004377"
     CDS             335..574
                     /locus_tag="OQM34_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS1454539.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(762..1739)
                     /gene="intI2"
                     /locus_tag="OQM34_004378"
                     /pseudo
     CDS             complement(762..1739)
                     /gene="intI2"
                     /locus_tag="OQM34_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2071..2544
                     /locus_tag="OQM34_004379"
     CDS             2071..2544
                     /locus_tag="OQM34_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1454540.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2639..3163
                     /gene="sat2"
                     /locus_tag="OQM34_004380"
     CDS             2639..3163
                     /gene="sat2"
                     /locus_tag="OQM34_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1454541.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT          942 a          710 c          714 g          905 t
ORIGIN      
        1 agctaacctg ccgctcagct tagtacgatc ctatatccaa ctgatcataa tcctttcatc
       61 agtatcccaa attaaatccc cctcctagac tgcctcacca acactttaca gtgatcccgt
      121 atttccggac aggtgattga tttttttcgc ttgtacaggt gttaagccac agttatcttg
      181 atgtggacat tatgcattgt aatagcccat taacatgcgc taagagcata aattaatata
      241 cagtaacaag cattacgagg tgctaatgtt tggaaagcag cctcttccaa ctacaataat
      301 aagttaacta tcctccaccc acaaataatt acaaatgacc cctttccgct ataactccga
      361 tctcaccagc ggctcgctac aaacccgaga gtgtcgcatt attactggct tgttactcca
      421 agagcttgat gaagctgcct gggataaagc catgtataag gaaaatgtgc ttcagaaacg
      481 cacgcaatct acggtaagac gtatttcttc agcgcttaga aaacgcctag aacacctaag
      541 ctctgacttt tgggcttttg cgtttttatg ttaggcattg tctggcaact ggaggaagtg
      601 taatgaccat gaatggtcac tgtgattgac agtatcttgc ttaaacttat ctgaacaatg
      661 gtagaactgg ctcatattta tcccctctat gattgtttat ctttttcacc ataaaaagta
      721 tcaggggcta ctcagggcta cataaagata tttatctact tttactgatt gataagtagc
      781 atcagtccat ccgcaggact ggtggtgccg gcaaaatgct gacccaacac atgcgtatag
      841 atttgcgtgg tcttaacatc gttatgccct aagagttctt gcacagtgcg aatatcacgc
      901 cccgcttgta atagatgcgt agcaaacgag tgacgaaatg tatgacaagt gacacgcttg
      961 ctaacgatgc ctgctttttg tacggctgcc ttcaatgcct ttcgcgcaac ggagtcatgc
     1021 agatgatggc ggcataattt gccgttatac gggtggttgc agagcgtgct ggagggaaag
     1081 acaaacatcc acgccgcttg tcgataagca gaagggtatt tgtgatctaa agcaaaaggc
     1141 agcgatggcc ctacgccttg taagttgtcg tcttgctgaa taagccgcgc ttgctcaatg
     1201 agttatttta ttgctgggat taggcgcgtg ggcagtaggc tgtttctgct tttcccaccc
     1261 ttaccgtcat gcacagtgat gcagccatta tcaaaatcaa aatctttaac ccgcaaacgc
     1321 aagcattcat taatgcgcaa acctgcacca tacagcagcg taaaaataac ttggttgcga
     1381 gtatccataa cctgcaaaat gcgttgcact tcatttgcag agataacaga gggtagccgt
     1441 ctaggcttgc ttgcagggat ataatcaata tcgcccaacg gctgttgtaa aaacctgttg
     1501 tacaaaaaag ctagggcatt taaagcgatt ttctgcgtgt ttatggctac atgtctgctg
     1561 tttgctaagc tggataaaaa cagcctgacc tcttcactgc ccatggtctg aggatgacgt
     1621 tttttgtgaa acagaataaa acgcttaatc cagtgcaggt aagttttttc agttttcagc
     1681 gcataacctt tttgtcgcat atccgtgcgt atagaattta aaaatggact gttagacata
     1741 aaacgctcct tgtcttgcaa ctgtctgcct atacagccta ttctagctgg gatttaaaaa
     1801 agtgcctgtt ttttacgcct agagatgctt gtttaccggt agagttttaa tttaatgcta
     1861 aataaattaa aatgttatga gttctttggg tgagataatg tgcatcgtgc aagcaggata
     1921 gacggcatgc acgatttgta ataacagagt gtcttgtatt tttaaagaaa gtctatttaa
     1981 tacaagtgat tatattaatt aacggtaagc atcagcgggt gacaaaacga gcatgcttac
     2041 taataaaatg ttaacctctg aggaagaatt gtgaaactat cactaatggt agctatatcg
     2101 aagaatggag ttatcgggaa tggccctgat attccatgga gtgccaaagg tgaacagctc
     2161 ctgtttaaag ctattaccta taaccaatgg ctgttggttg gacgcaagac ttttgaatca
     2221 atgggagcat tacccaaccg aaagtatgcg gtcgtaacac gttcaagttt tacatctgac
     2281 aatgagaacg tattgatctt tccatcaatt aaagatgctt taaccaacct aaagaaaata
     2341 acggatcatg tcattgtttc aggtggtggg gagatataca aaagcctgat cgatcaagta
     2401 gatacactac atatatctac aatagacatc gagccggaag gtgatgttta ctttcctgaa
     2461 atccccagca attttaggcc agtttttacc caagacttcg cctctaacat aaattatagt
     2521 taccaaatct ggcaaaaggg ttaacaagtg gcagcaacgg attcgcaaac ctgtcacgcc
     2581 ttttgtacca aaagccgcgc caggtttgcg atccgctgtg ccaggcgtta ggcgtcatat
     2641 gaagatttcg gtgatccctg agcaggtggc ggaaacattg gatgctgaga accatttcat
     2701 tgttcgtgaa gtgttcgatg tgcacctatc cgaccaaggc tttgaactat ctaccagaag
     2761 tgtgagcccc taccggaagg attacatctc ggatgatgac tctgatgaag actctgcttg
     2821 ctatggcgca ttcatcgacc aagagcttgt cgggaagatt gaactcaact caacatggaa
     2881 cgatctagcc tctatcgaac acattgttgt gtcgcacacg caccgaggca aaggagtcgc
     2941 gcacagtctc atcgaatttg cgaaaaagtg ggcactaagc agacagctcc ttggcatacg
     3001 attagagaca caaacgaaca atgtacctgc ctgcaatttg tacgcaaaat gtggctttac
     3061 tctcggcggc attgacctgt tcacgtataa aactagacct caagtctcga acgaaacagc
     3121 gatgtactgg tactggttct cgggagcaca ggatgacgcc taacaattca ttcaagccga
     3181 caccgcttcg cggcgcggct taattcagga gttaaacatc atgagggaag cggtgatcgc
     3241 cgaagtatcg actcaactat cagaggtagt t
//
LOCUS       DAJVPP010000423         3271 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.212, whole genome shotgun sequence.
ACCESSION   DAJVPP010000423 DAJVPP010000000
VERSION     DAJVPP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.212"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            335..574
                     /locus_tag="OQM34_004381"
     CDS             335..574
                     /locus_tag="OQM34_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS1454542.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(762..1739)
                     /gene="intI2"
                     /locus_tag="OQM34_004382"
                     /pseudo
     CDS             complement(762..1739)
                     /gene="intI2"
                     /locus_tag="OQM34_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2071..2544
                     /locus_tag="OQM34_004383"
     CDS             2071..2544
                     /locus_tag="OQM34_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1454543.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2639..3163
                     /gene="sat2"
                     /locus_tag="OQM34_004384"
     CDS             2639..3163
                     /gene="sat2"
                     /locus_tag="OQM34_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1454544.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT          942 a          709 c          714 g          906 t
ORIGIN      
        1 agctaacctg ccgctcagct tagtacgatc ctatatccaa ctgatcataa tcctttcatc
       61 agtatcccaa attaaatccc cctcctagac tgcctcacca acactttaca gtgatcccgt
      121 atttccggac aggtgattga tttttttcgc ttgtacaggt gttaagccac agttatcttg
      181 atgtggacat tatgcattgt aatagcccat taacatgcgc taagagcata aattaatata
      241 cagtaacaag cattacgagg tgctaatgtt tggaaagcag cctcttccaa ctacaataat
      301 aagttaacta tcctccaccc acaaataatt acaaatgacc cctttccgct ataactccga
      361 tctcaccagc ggctcgctac aaacccgaga gtgtcgcatt attactggct tgttactcca
      421 agagcttgat gaagctgcct gggataaagc catgtataag gaaaatgtgc ttcagaaacg
      481 cacgcaatct acggtaagac gtatttcttc agcgcttaga aaacgcctag aacacctaag
      541 ctctgacttt tgggcttttg cgtttttatg ttaggcattg tctggcaact ggaggaagtg
      601 taatgaccat gaatggtcac tgtgattgac agtatcttgc ttaaacttat ctgaacaatg
      661 gtagaactgg ctcatattta tcccctctat gattgtttat ctttttcacc ataaaaagta
      721 tcaggggcta ctcagggcta cataaagata tttatctact tttactgatt gataagtagc
      781 atcagtccat ccgcaggact ggtggtgccg gcaaaatgct gacccaacac atgcgtatag
      841 atttgcgtgg tcttaacatc gttatgccct aagagttctt gcacagtgcg aatatcacgc
      901 cccgcttgta atagatgcgt agcaaacgag tgacgaaatg tatgacaagt gacacgcttg
      961 ctaacgatgc ctgctttttg tacggctgcc ttcaatgcct ttcgcgcaac ggagtcatgc
     1021 agatgatggc ggcataattt gccgttatac gggtggttgc agagcgtgct ggagggaaag
     1081 acaaacatcc acgccgcttg tcgataagca gaagggtatt tgtgatctaa agcaaaaggc
     1141 agcgatggcc ctacgccttg taagttgtcg tcttgctgaa taagccgcgc ttgctcaatg
     1201 agttatttta ttgctgggat taggcgcgtg ggcagtaggc tgtttctgct tttcccaccc
     1261 ttaccgtcat gcacagtgat gcagccatta tcaaaatcaa aatctttaac ccgcaaacgc
     1321 aagcattcat taatgcgcaa acctgcacca tacagcagcg taaaaataac ttggttgcga
     1381 gtatccataa cctgcaaaat gcgttgcact tcatttgcag agataacaga gggtagccgt
     1441 ctaggcttgc ttgcagggat ataatcaata tcgcccaacg gctgttgtaa aaacctgttg
     1501 tacaaaaaag ctagggcatt taaagcgatt ttctgcgtgt ttatggctac atgtctgctg
     1561 tttgctaagc tggataaaaa cagcctgacc tcttcactgc ccatggtctg aggatgacgt
     1621 tttttgtgaa acagaataaa acgcttaatc cagtgcaggt aagttttttc agttttcagc
     1681 gcataacctt tttgtcgcat atccgtgcgt atagaattta aaaatggact gttagacata
     1741 aaacgctcct tgtcttgcaa ctgtctgcct atacagccta ttctagctgg gatttaaaaa
     1801 agtgcctgtt ttttacgcct agagatgctt gtttaccggt agagttttaa tttaatgcta
     1861 aataaattaa aatgttatga gttctttggg tgagataatg tgcatcgtgc aagcaggata
     1921 gacggcatgc acgatttgta ataacagagt gtcttgtatt tttaaagaaa gtctatttaa
     1981 tacaagtgat tatattaatt aacggtaagc atcagcgggt gacaaaacga gcatgcttac
     2041 taataaaatg ttaacctctg aggaagaatt gtgaaactat cactaatggt agctatatcg
     2101 aagaatggag ttatcgggaa tggccctgat attccatgga gtgccaaagg tgaacagctc
     2161 ctgtttaaag ctattaccta taaccaatgg ctgttggttg gacgcaagac ttttgaatca
     2221 atgggagcat tacccaaccg aaagtatgcg gtcgtaacac gttcaagttt tacatctgac
     2281 aatgagaacg tattgatctt tccatcaatt aaagatgctt taaccaacct aaagaaaata
     2341 acggatcatg tcattgtttc aggtggtggg gagatataca aaagcctgat cgatcaagta
     2401 gatacactac atatatctac aatagacatc gagccggaag gtgatgttta ctttcctgaa
     2461 atccccagca attttaggcc agtttttacc caagacttcg cttctaacat aaattatagt
     2521 taccaaatct ggcaaaaggg ttaacaagtg gcagcaacgg attcgcaaac ctgtcacgcc
     2581 ttttgtacca aaagccgcgc caggtttgcg atccgctgtg ccaggcgtta ggcgtcatat
     2641 gaagatttcg gtgatccctg agcaggtggc ggaaacattg gatgctgaga accatttcat
     2701 tgttcgtgaa gtgttcgatg tgcacctatc cgaccaaggc tttgaactat ctaccagaag
     2761 tgtgagcccc taccggaagg attacatctc ggatgatgac tctgatgaag actctgcttg
     2821 ctatggcgca ttcatcgacc aagagcttgt cgggaagatt gaactcaact caacatggaa
     2881 cgatctagcc tctatcgaac acattgttgt gtcgcacacg caccgaggca aaggagtcgc
     2941 gcacagtctc atcgaatttg cgaaaaagtg ggcactaagc agacagctcc ttggcatacg
     3001 attagagaca caaacgaaca atgtacctgc ctgcaatttg tacgcaaaat gtggctttac
     3061 tctcggcggc attgacctgt tcacgtataa aactagacct caagtctcga acgaaacagc
     3121 gatgtactgg tactggttct cgggagcaca ggatgacgcc taacaattca ttcaagccga
     3181 caccgcttcg cggcgcggct taattcagga gttaaacatc atgagggaag cggtgatcgc
     3241 cgaagtatcg actcaactat cagaggtagt t
//
LOCUS       DAJVPP010000424         1567 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.246, whole genome shotgun sequence.
ACCESSION   DAJVPP010000424 DAJVPP010000000
VERSION     DAJVPP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.246"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            137..661
                     /gene="sat2"
                     /locus_tag="OQM34_004385"
     CDS             137..661
                     /gene="sat2"
                     /locus_tag="OQM34_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1454545.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            719..1507
                     /gene="aadA1"
                     /locus_tag="OQM34_004386"
     CDS             719..1507
                     /gene="aadA1"
                     /locus_tag="OQM34_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1454546.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          405 a          388 c          413 g          361 t
ORIGIN      
        1 tctaacataa attatagtta ccaaatctgg caaaagggtt aacaagtggc agcaacggat
       61 tcgcaaacct gtcacgcctt ttgtaccaaa agccgcgcca ggtttgcgat ccgctgtgcc
      121 aggcgttagg cgtcatatga agatttcggt gatccctgag caggtggcgg aaacattgga
      181 tgctgagaac catttcattg ttcgtgaagt gttcgatgtg cacctatccg accaaggctt
      241 tgaactatct accagaagtg tgagccccta ccggaaggat tacatctcgg atgatgactc
      301 tgatgaagac tctgcttgct atggcgcatt catcgaccaa gagcttgtcg ggaagattga
      361 actcaactca acatggaacg atctagcctc tatcgaacac attgttgtgt cgcacacgca
      421 ccgaggcaaa ggagtcgcgc acagtctcat cgaatttgcg aaaaagtggg cactaagcag
      481 acagctcctt ggcatacgat tagagacaca aacgaacaat gtacctgcct gcaatttgta
      541 cgcaaaatgt ggctttactc tcggcggcat tgacctgttc acgtataaaa ctagacctca
      601 agtctcgaac gaaacagcga tgtactggta ctggttctcg ggagcacagg atgacgccta
      661 acaattcatt caagccgaca ccgcttcgcg gcgcggctta attcaggagt taaacatcat
      721 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
      781 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
      841 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
      901 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
      961 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     1021 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     1081 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     1141 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     1201 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     1261 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     1321 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     1381 gcccgtcata cttgaagcta ggcaggctta tcttggacaa gaagatcgct tggcctcgcg
     1441 cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg
     1501 caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     1561 cgttaga
//
LOCUS       DAJVPP010000425         1358 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.252, whole genome shotgun sequence.
ACCESSION   DAJVPP010000425 DAJVPP010000000
VERSION     DAJVPP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.252"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            264..923
                     /locus_tag="OQM34_004387"
     CDS             264..923
                     /locus_tag="OQM34_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCS1454547.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..>1358)
                     /locus_tag="OQM34_004388"
     CDS             complement(1124..>1358)
                     /locus_tag="OQM34_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1454548.1"
                     /translation="KELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPG
                     ANRFYQALGGRINDHIVNYYLHGESLSKLASGI"
BASE COUNT          361 a          302 c          317 g          378 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctcttta
//
LOCUS       DAJVPP010000426         1238 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.256, whole genome shotgun sequence.
ACCESSION   DAJVPP010000426 DAJVPP010000000
VERSION     DAJVPP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.256"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            <1..277
                     /locus_tag="OQM34_004389"
     CDS             <1..277
                     /locus_tag="OQM34_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCS1454549.1"
                     /translation="NLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNG
                     WFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            390..1178
                     /gene="aadA1"
                     /locus_tag="OQM34_004390"
     CDS             390..1178
                     /gene="aadA1"
                     /locus_tag="OQM34_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1454550.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          348 a          297 c          309 g          284 t
ORIGIN      
        1 caatctccca gttaaaaact cagccataga aaacaccata gagaacatgt atctacaaga
       61 tctggataat agtacaaaac tgtatgggaa aactggtgca ggattcacag caaatagaac
      121 cttacaaaac ggatggtttg aagggtttat tataagcaaa tcaggacata aatatgtttt
      181 tgtgtccgca cttacaggaa acttggggtc gaatttaaca tcaagcataa aagccaagaa
      241 aaatgcgatc accattctaa acacactaaa tttataaaaa atctaatggc aaaatcgccc
      301 aacccttcaa tcaagtcggg acggccaaaa gcaagctttt ggctcccctc gctggcgctc
      361 ggcgcccctt atttcaaacg ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga
      421 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
      481 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
      541 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
      601 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
      661 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
      721 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
      781 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
      841 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
      901 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
      961 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1021 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt
     1081 atcttggaca agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact
     1141 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc
     1201 gacgccgctt cgcggcgcgg cttaactcaa gcgttaga
//
LOCUS       DAJVPP010000427         1086 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702958
            SAMEA8900963-rid15852693.guided.265, whole genome shotgun sequence.
ACCESSION   DAJVPP010000427 DAJVPP010000000
VERSION     DAJVPP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900963
            Sequence Read Archive: ERR6005158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478072.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478072.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:35:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,391
            CDSs (total)                      :: 4,321
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 176 of 370
            Pseudo Genes (incomplete)         :: 81 of 370
            Pseudo Genes (internal stop)      :: 149 of 370
            Pseudo Genes (multiple problems)  :: 34 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900963-rid15852693.guided.265"
                     /serovar="S. flexneri 4a"
                     /isolate="702958"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-01-26"
     gene            93..923
                     /locus_tag="OQM34_004391"
     CDS             93..923
                     /locus_tag="OQM34_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCS1454551.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          401 a          216 c          192 g          277 t
ORIGIN      
        1 gggcagtcgc cctaaaacaa agttgggcga acccggagcc tcattaattg ttagccgtta
       61 aaattaagcc ctttaccaaa ccaatactta ttatgaaaaa cacaatacat atcaacttcg
      121 ctattttttt aataattgca aatattatct acagcagcgc cagtgcatca acagatatct
      181 ctactgttgc atctccatta tttgaaggaa ctgaaggttg ttttttactt tacgatgcat
      241 ccacaaacgc tgaaattgct caattcaata aagcaaagtg tgcaacgcaa atggcaccag
      301 attcaacttt caagatcgca ttatcactta tggcatttga tgcggaaata atagatcaga
      361 aaaccatatt caaatgggat aaaaccccca aaggaatgga gatctggaac agcaatcata
      421 caccaaagac gtggatgcaa ttttctgttg tttgggtttc gcaagaaata acccaaaaaa
      481 ttggattaaa taaaatcaag aattatctca aagattttga ttatggaaat caagacttct
      541 ctggagataa agaaagaaac aacggattaa cagaagcatg gctcgaaagt agcttaaaaa
      601 tttcaccaga agaacaaatt caattcctgc gtaaaattat taatcacaat ctcccagtta
      661 aaaactcagc catagaaaac accatagaga acatgtatct acaagatctg gataatagta
      721 caaaactgta tgggaaaact ggtgcaggat tcacagcaaa tagaacctta caaaacggat
      781 ggtttgaagg gtttattata agcaaatcag gacataaata tgtttttgtg tccgcactta
      841 caggaaactt ggggtcgaat ttaacatcaa gcataaaagc caagaaaaat gcgatcacca
      901 ttctaaacac actaaattta taaaaaatct aatggcaaaa tcgcccaacc cttcaatcaa
      961 gtcgggacgg ccaaaagcaa gcttttggct cccctcgctg gcgctcggcg ccccttattt
     1021 caaacgttaa acatcatgag ggaagtggtg atcgccgaag tatcgactca actatcagag
     1081 gtagtt
//
